LOCUS       DAJVYM010000001        87171 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVYM010000001 DAJVYM010000000
VERSION     DAJVYM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 87171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.001"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OQS60_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OQS60_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS2385882.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OQS60_000002"
     tRNA            1230..1305
                     /locus_tag="OQS60_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OQS60_000003"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OQS60_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OQS60_000004"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OQS60_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS2385883.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OQS60_000005"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OQS60_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS2385884.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OQS60_000006"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OQS60_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS2385885.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="OQS60_000007"
     CDS             complement(4869..5306)
                     /locus_tag="OQS60_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS2385886.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="OQS60_000008"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="OQS60_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OQS60_000009"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OQS60_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCS2385887.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="OQS60_000010"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="OQS60_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCS2385888.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="OQS60_000011"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="OQS60_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCS2385889.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="OQS60_000012"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="OQS60_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCS2385890.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="OQS60_000013"
     CDS             10774..11442
                     /locus_tag="OQS60_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCS2385891.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="OQS60_000014"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="OQS60_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCS2385892.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="OQS60_000015"
     CDS             11985..13817
                     /locus_tag="OQS60_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCS2385893.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="OQS60_000016"
     CDS             13807..14157
                     /locus_tag="OQS60_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCS2385894.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="OQS60_000017"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="OQS60_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCS2385895.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="OQS60_000018"
     CDS             14476..14898
                     /locus_tag="OQS60_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCS2385896.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="OQS60_000019"
     CDS             14901..15533
                     /locus_tag="OQS60_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCS2385897.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="OQS60_000020"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="OQS60_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCS2385898.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="OQS60_000021"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="OQS60_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCS2385899.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="OQS60_000022"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="OQS60_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCS2385900.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="OQS60_000023"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="OQS60_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="OQS60_000024"
     CDS             18712..19824
                     /locus_tag="OQS60_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCS2385901.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="OQS60_000025"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="OQS60_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="OQS60_000026"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="OQS60_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCS2385902.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="OQS60_000027"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="OQS60_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCS2385903.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /locus_tag="OQS60_000028"
     CDS             22078..22521
                     /locus_tag="OQS60_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCS2385904.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="OQS60_000029"
     CDS             complement(22622..22951)
                     /locus_tag="OQS60_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS2385905.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="OQS60_000030"
     CDS             complement(23123..24181)
                     /locus_tag="OQS60_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS2385906.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="OQS60_000031"
     CDS             complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="OQS60_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCS2385907.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OQS60_000032"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OQS60_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCS2385908.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="OQS60_000033"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="OQS60_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS2385909.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /locus_tag="OQS60_000034"
     CDS             complement(27816..28043)
                     /locus_tag="OQS60_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS2385910.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OQS60_000035"
     CDS             complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OQS60_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS2385911.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OQS60_000036"
     CDS             complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OQS60_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS2385912.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OQS60_000037"
     CDS             complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OQS60_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS2385913.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30919..31848)
                     /locus_tag="OQS60_000038"
     CDS             complement(30919..31848)
                     /locus_tag="OQS60_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2385914.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31894..32718)
                     /locus_tag="OQS60_000039"
     CDS             complement(31894..32718)
                     /locus_tag="OQS60_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2385915.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OQS60_000040"
     CDS             complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OQS60_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS2385916.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OQS60_000041"
     CDS             complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OQS60_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS2385917.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33586..33636
                     /gene="hisL"
                     /locus_tag="OQS60_000042"
     CDS             33586..33636
                     /gene="hisL"
                     /locus_tag="OQS60_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS2385918.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33612..33735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33782..34681
                     /gene="hisG"
                     /locus_tag="OQS60_000043"
     CDS             33782..34681
                     /gene="hisG"
                     /locus_tag="OQS60_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS2385919.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34687..35991
                     /gene="hisD"
                     /locus_tag="OQS60_000044"
     CDS             34687..35991
                     /gene="hisD"
                     /locus_tag="OQS60_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS2385920.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35988..37058
                     /gene="hisC"
                     /locus_tag="OQS60_000045"
     CDS             35988..37058
                     /gene="hisC"
                     /locus_tag="OQS60_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS2385921.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37058..38125
                     /gene="hisB"
                     /locus_tag="OQS60_000046"
     CDS             37058..38125
                     /gene="hisB"
                     /locus_tag="OQS60_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS2385922.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38125..38715
                     /gene="hisH"
                     /locus_tag="OQS60_000047"
     CDS             38125..38715
                     /gene="hisH"
                     /locus_tag="OQS60_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS2385923.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38715..39452
                     /gene="hisA"
                     /locus_tag="OQS60_000048"
     CDS             38715..39452
                     /gene="hisA"
                     /locus_tag="OQS60_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS2385924.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39434..40210
                     /gene="hisF"
                     /locus_tag="OQS60_000049"
     CDS             39434..40210
                     /gene="hisF"
                     /locus_tag="OQS60_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS2385925.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40204..40815
                     /locus_tag="OQS60_000050"
     CDS             40204..40815
                     /locus_tag="OQS60_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS2385926.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OQS60_000051"
     CDS             complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OQS60_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS2385927.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OQS60_000052"
     CDS             complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OQS60_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS2385928.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OQS60_000053"
     CDS             complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OQS60_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS2385929.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OQS60_000054"
                     /gene_synonym="manB"
     CDS             complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OQS60_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS2385930.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OQS60_000055"
     CDS             complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OQS60_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS2385931.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OQS60_000056"
     CDS             complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OQS60_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS2385932.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OQS60_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OQS60_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS2385933.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OQS60_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OQS60_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS2385934.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTLPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OQS60_000059"
     CDS             complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OQS60_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS2385935.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OQS60_000060"
     CDS             complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OQS60_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS2385936.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OQS60_000061"
     CDS             complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OQS60_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS2385937.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OQS60_000062"
     CDS             complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OQS60_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS2385938.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OQS60_000063"
     CDS             complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OQS60_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS2385939.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OQS60_000064"
     CDS             complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OQS60_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS2385940.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OQS60_000065"
     CDS             complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OQS60_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS2385941.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OQS60_000066"
     CDS             complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OQS60_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS2385942.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OQS60_000067"
     CDS             complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OQS60_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS2385943.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OQS60_000068"
     CDS             complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OQS60_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS2385944.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60964..61113
                     /locus_tag="OQS60_000069"
     CDS             60964..61113
                     /locus_tag="OQS60_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2385945.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61314..62897
                     /locus_tag="OQS60_000070"
     CDS             61314..62897
                     /locus_tag="OQS60_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS2385946.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63159..65024)
                     /gene="asmA"
                     /locus_tag="OQS60_000071"
     CDS             complement(63159..65024)
                     /gene="asmA"
                     /locus_tag="OQS60_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS2385947.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OQS60_000072"
     CDS             complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OQS60_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS2385948.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OQS60_000073"
     CDS             complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OQS60_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS2385949.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66678..69995
                     /locus_tag="OQS60_000074"
     CDS             66678..69995
                     /locus_tag="OQS60_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS2385950.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OQS60_000075"
     CDS             complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OQS60_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS2385951.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71087..72439
                     /gene="yegD"
                     /locus_tag="OQS60_000076"
     CDS             71087..72439
                     /gene="yegD"
                     /locus_tag="OQS60_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2385952.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OQS60_000077"
     CDS             complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OQS60_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS2385953.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OQS60_000078"
     CDS             complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OQS60_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS2385954.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75147..75806)
                     /locus_tag="OQS60_000079"
     CDS             complement(75147..75806)
                     /locus_tag="OQS60_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS2385955.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OQS60_000080"
     CDS             complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OQS60_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS2385956.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76359..76415)
                     /locus_tag="OQS60_000081"
     CDS             complement(76359..76415)
                     /locus_tag="OQS60_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2385957.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76686..76742)
                     /locus_tag="OQS60_000082"
     CDS             complement(76686..76742)
                     /locus_tag="OQS60_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2385958.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77021..78262
                     /gene="mdtA"
                     /locus_tag="OQS60_000083"
     CDS             77021..78262
                     /gene="mdtA"
                     /locus_tag="OQS60_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS2385959.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78262..81384
                     /gene="mdtB"
                     /locus_tag="OQS60_000084"
     CDS             78262..81384
                     /gene="mdtB"
                     /locus_tag="OQS60_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS2385960.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81385..84462
                     /gene="mdtC"
                     /locus_tag="OQS60_000085"
     CDS             81385..84462
                     /gene="mdtC"
                     /locus_tag="OQS60_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS2385961.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84463..85878
                     /locus_tag="OQS60_000086"
     CDS             84463..85878
                     /locus_tag="OQS60_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2385962.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85875..>87171
                     /gene="baeS"
                     /locus_tag="OQS60_000087"
     CDS             85875..>87171
                     /gene="baeS"
                     /locus_tag="OQS60_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS2385963.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLN"
BASE COUNT        21348 a        23074 c        21908 g        20841 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcga tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatt gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    29221 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    29281 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    29341 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    29401 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    29461 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    29521 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    29581 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    29641 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    29701 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    29761 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    29821 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    29881 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    29941 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    30001 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    30061 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    30121 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    30181 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    30241 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    30301 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    30361 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    30421 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    30481 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    30541 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    30601 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    30661 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    30721 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    30781 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    30841 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    30901 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    30961 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    31021 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    31081 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    31141 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    31201 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    31261 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    31321 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    31381 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    31441 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    31501 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    31561 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    31621 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    31681 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    31741 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    31801 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    31861 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    31921 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    31981 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    32041 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    32101 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    32161 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    32221 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    32281 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    32341 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    32401 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    32461 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    32521 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    32581 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    32641 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    32701 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    32761 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    32821 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    32881 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    32941 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    33001 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    33061 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    33121 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    33181 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    33241 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    33301 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    33361 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    33421 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    33481 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    33541 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    33601 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    33661 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    33721 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    33781 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    33841 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    33901 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    33961 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    34021 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    34081 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    34141 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    34201 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    34261 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    34321 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    34381 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    34441 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    34501 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    34561 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    34621 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    34681 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    34741 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    34801 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    34861 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    34921 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    34981 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    35041 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    35101 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    35161 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    35221 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    35281 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    35341 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    35401 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    35461 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    35521 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    35581 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    35641 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    35701 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    35761 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    35821 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    35881 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    35941 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    36001 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    36061 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    36121 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    36181 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    36241 acgaaggtat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct
    36301 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    36361 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    36421 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    36481 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    36541 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    36601 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    36661 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    36721 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    36781 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    36841 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    36901 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    36961 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    37021 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    37081 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    37141 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    37201 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    37261 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    37321 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    37381 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    37441 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    37501 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    37561 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    37621 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    37681 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    37741 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    37801 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    37861 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    37921 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    37981 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    38041 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    38101 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    38161 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    38221 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    38281 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    38341 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    38401 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    38461 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    38521 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    38581 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    38641 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    38701 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    38761 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    38821 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    38881 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    38941 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    39001 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    39061 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    39121 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    39181 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    39241 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    39301 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    39361 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    39421 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    39481 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    39541 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    39601 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    39661 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    39721 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    39781 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    39841 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    39901 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    39961 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    40021 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    40081 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    40141 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    40201 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    40261 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    40321 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    40381 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    40441 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    40501 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    40561 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    40621 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    40681 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    40741 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    40801 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    40861 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    40921 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    40981 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    41041 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    41101 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    41161 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    41221 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    41281 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    41341 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    41401 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    41461 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    41521 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    41581 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    41641 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    41701 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    41761 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcactag
    41821 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    41881 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    41941 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    42001 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    42061 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    42121 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    42181 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    42241 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    42301 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    42361 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    42421 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    42481 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    42541 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    42601 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    42661 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    42721 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    42781 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    42841 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    42901 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    42961 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    43021 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    43081 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    43141 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    43201 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    43261 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    43321 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    43381 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    43441 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    43501 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    43561 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    43621 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    43681 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    43741 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    43801 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    43861 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    43921 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    43981 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    44041 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    44101 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    44161 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    44221 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    44281 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    44341 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    44401 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    44461 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    44521 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    44581 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    44641 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    44701 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    44761 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    44821 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    44881 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    44941 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    45001 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    45061 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    45121 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    45181 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    45241 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    45301 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    45361 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    45421 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    45481 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    45541 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    45601 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    45661 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    45721 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    45781 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    45841 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    45901 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    45961 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    46021 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    46081 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    46141 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    46201 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    46261 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    46321 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    46381 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    46441 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    46501 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    46561 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    46621 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    46681 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    46741 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    46801 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    46861 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    46921 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    46981 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    47041 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    47101 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    47161 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    47221 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    47281 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    47341 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    47401 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    47461 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    47521 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    47581 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    47641 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    47701 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    47761 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    47821 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    47881 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    47941 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    48001 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    48061 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    48121 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    48181 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    48241 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    48301 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    48361 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    48421 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    48481 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    48541 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    48601 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    48661 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    48721 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    48781 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    48841 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    48901 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    48961 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    49021 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    49081 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    49141 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    49201 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    49261 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    49321 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    49381 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    49441 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    49501 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    49561 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    49621 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    49681 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    49741 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    49801 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    49861 ccgtgccgac gttaatgtgc gacagcatcg gcagggtgtt ctccagccag acttcgtgcg
    49921 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    49981 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    50041 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    50101 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    50161 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    50221 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    50281 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    50341 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    50401 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    50461 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    50521 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    50581 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    50641 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    50701 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    50761 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    50821 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    50881 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    50941 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    51001 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    51061 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    51121 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    51181 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    51241 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    51301 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    51361 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    51421 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    51481 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    51541 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    51601 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    51661 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    51721 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    51781 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    51841 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    51901 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    51961 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    52021 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    52081 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    52141 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    52201 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    52261 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    52321 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    52381 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    52441 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    52501 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    52561 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    52621 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    52681 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    52741 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    52801 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    52861 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    52921 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    52981 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    53041 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    53101 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    53161 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    53221 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    53281 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    53341 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    53401 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    53461 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    53521 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    53581 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    53641 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    53701 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    53761 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    53821 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    53881 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    53941 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    54001 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    54061 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    54121 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    54181 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    54241 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    54301 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    54361 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    54421 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    54481 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    54541 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    54601 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    54661 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    54721 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    54781 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    54841 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    54901 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    54961 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    55021 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    55081 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    55141 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    55201 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    55261 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    55321 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    55381 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    55441 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    55501 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    55561 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    55621 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    55681 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    55741 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    55801 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    55861 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    55921 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    55981 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    56041 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    56101 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    56161 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    56221 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    56281 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    56341 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    56401 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    56461 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    56521 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    56581 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    56641 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    56701 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    56761 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    56821 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    56881 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    56941 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    57001 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    57061 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    57121 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    57181 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    57241 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    57301 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    57361 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    57421 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    57481 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    57541 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    57601 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    57661 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    57721 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    57781 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    57841 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    57901 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    57961 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    58021 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    58081 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    58141 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    58201 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    58261 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    58321 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    58381 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    58441 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    58501 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    58561 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    58621 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    58681 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    58741 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    58801 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    58861 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    58921 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    58981 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    59041 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    59101 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    59161 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    59221 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    59281 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    59341 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    59401 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    59461 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    59521 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    59581 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    59641 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    59701 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    59761 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    59821 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    59881 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    59941 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    60001 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    60061 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    60121 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    60181 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    60241 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    60301 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    60361 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    60421 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    60481 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    60541 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    60601 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    60661 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    60721 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    60781 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    60841 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    60901 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    60961 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    61021 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    61081 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    61141 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    61201 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    61261 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    61321 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    61381 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    61441 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    61501 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    61561 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    61621 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    61681 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    61741 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    61801 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    61861 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    61921 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    61981 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    62041 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    62101 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    62161 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    62221 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    62281 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    62341 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    62401 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    62461 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    62521 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    62581 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    62641 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    62701 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    62761 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    62821 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    62881 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    62941 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    63001 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    63061 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    63121 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac caacatcttc
    63181 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    63241 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    63301 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    63361 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    63421 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    63481 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    63541 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    63601 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    63661 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    63721 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    63781 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    63841 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    63901 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    63961 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    64021 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    64081 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    64141 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    64201 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    64261 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    64321 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    64381 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    64441 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    64501 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    64561 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    64621 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    64681 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    64741 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    64801 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    64861 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    64921 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    64981 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    65041 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    65101 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    65161 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    65221 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    65281 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    65341 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    65401 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    65461 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    65521 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    65581 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    65641 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    65701 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    65761 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    65821 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    65881 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    65941 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    66001 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    66061 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    66121 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    66181 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    66241 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    66301 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    66361 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    66421 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    66481 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    66541 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    66601 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    66661 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    66721 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    66781 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    66841 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    66901 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    66961 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    67021 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    67081 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    67141 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    67201 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    67261 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    67321 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    67381 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    67441 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    67501 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    67561 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    67621 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    67681 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    67741 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    67801 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    67861 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    67921 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    67981 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    68041 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    68101 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    68161 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    68221 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    68281 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    68341 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    68401 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    68461 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    68521 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    68581 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    68641 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    68701 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    68761 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    68821 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    68881 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    68941 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    69001 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    69061 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    69121 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    69181 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    69241 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    69301 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    69361 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    69421 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    69481 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    69541 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    69601 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    69661 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    69721 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    69781 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    69841 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    69901 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    69961 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    70021 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    70081 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    70141 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    70201 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    70261 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    70321 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    70381 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    70441 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    70501 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    70561 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    70621 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    70681 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    70741 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    70801 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    70861 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    70921 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    70981 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    71041 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    71101 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    71161 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    71221 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    71281 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    71341 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    71401 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    71461 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    71521 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    71581 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    71641 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    71701 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    71761 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    71821 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    71881 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    71941 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    72001 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    72061 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    72121 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    72181 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    72241 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    72301 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    72361 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    72421 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    72481 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    72541 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    72601 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    72661 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    72721 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    72781 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    72841 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    72901 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    72961 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    73021 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    73081 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    73141 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    73201 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    73261 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    73321 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    73381 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    73441 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    73501 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    73561 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    73621 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    73681 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    73741 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    73801 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    73861 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    73921 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    73981 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    74041 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    74101 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    74161 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    74221 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    74281 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    74341 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    74401 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    74461 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    74521 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    74581 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    74641 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    74701 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    74761 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    74821 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    74881 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    74941 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    75001 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    75061 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    75121 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    75181 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    75241 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    75301 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    75361 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    75421 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    75481 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    75541 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    75601 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    75661 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    75721 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    75781 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    75841 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    75901 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    75961 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    76021 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    76081 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    76141 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    76201 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    76261 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    76321 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    76381 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    76441 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    76501 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    76561 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    76621 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    76681 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    76741 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    76801 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    76861 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    76921 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    76981 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    77041 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    77101 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    77161 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    77221 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    77281 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    77341 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    77401 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    77461 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    77521 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    77581 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    77641 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    77701 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    77761 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    77821 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    77881 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    77941 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    78001 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    78061 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    78121 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    78181 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    78241 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    78301 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    78361 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    78421 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    78481 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    78541 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    78601 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    78661 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    78721 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    78781 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    78841 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    78901 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    78961 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    79021 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    79081 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    79141 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    79201 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    79261 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    79321 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    79381 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    79441 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    79501 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    79561 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    79621 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    79681 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    79741 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    79801 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    79861 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    79921 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    79981 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    80041 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    80101 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    80161 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    80221 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    80281 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    80341 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    80401 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    80461 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    80521 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    80581 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    80641 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    80701 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    80761 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    80821 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    80881 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    80941 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    81001 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    81061 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    81121 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    81181 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    81241 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    81301 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    81361 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    81421 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    81481 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    81541 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    81601 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    81661 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    81721 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    81781 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    81841 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    81901 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    81961 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    82021 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    82081 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    82141 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    82201 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    82261 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    82321 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    82381 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    82441 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    82501 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    82561 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    82621 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    82681 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    82741 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    82801 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    82861 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    82921 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    82981 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    83041 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    83101 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    83161 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    83221 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    83281 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    83341 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    83401 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    83461 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    83521 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    83581 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    83641 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    83701 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    83761 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    83821 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    83881 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    83941 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    84001 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    84061 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    84121 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    84181 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    84241 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    84301 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    84361 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    84421 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    84481 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    84541 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    84601 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    84661 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    84721 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    84781 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    84841 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    84901 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    84961 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    85021 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    85081 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    85141 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    85201 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    85261 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    85321 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    85381 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    85441 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    85501 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    85561 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    85621 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    85681 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    85741 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    85801 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    85861 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    85921 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    85981 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    86041 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    86101 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    86161 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    86221 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    86281 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    86341 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    86401 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    86461 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    86521 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    86581 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    86641 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    86701 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    86761 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    86821 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    86881 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    86941 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    87001 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    87061 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    87121 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac a
//
LOCUS       DAJVYM010000002        79578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVYM010000002 DAJVYM010000000
VERSION     DAJVYM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.002"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="OQS60_000088"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="OQS60_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="OQS60_000089"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="OQS60_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCS2385964.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="OQS60_000090"
     CDS             2354..2758
                     /locus_tag="OQS60_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCS2385965.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="OQS60_000091"
     CDS             2811..2921
                     /locus_tag="OQS60_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2385966.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="OQS60_000092"
     CDS             3157..3201
                     /locus_tag="OQS60_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2385967.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="OQS60_000093"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="OQS60_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS2385968.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OQS60_000094"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OQS60_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS2385969.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="OQS60_000095"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="OQS60_000095"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS2385970.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="OQS60_000096"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="OQS60_000096"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS2385971.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="OQS60_000097"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="OQS60_000097"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS2385972.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="OQS60_000098"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="OQS60_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS2385973.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="OQS60_000099"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="OQS60_000099"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS2385974.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="OQS60_000100"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="OQS60_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS2385975.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="OQS60_000101"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="OQS60_000101"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS2385976.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="OQS60_000102"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="OQS60_000102"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS2385977.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OQS60_000103"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OQS60_000103"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS2385978.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="OQS60_000104"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="OQS60_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS2385979.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="OQS60_000105"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="OQS60_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS2385980.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="OQS60_000106"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="OQS60_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS2385981.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="OQS60_000107"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="OQS60_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS2385982.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OQS60_000108"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OQS60_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS2385983.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OQS60_000109"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OQS60_000109"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS2385984.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OQS60_000110"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OQS60_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS2385985.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OQS60_000111"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OQS60_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS2385986.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OQS60_000112"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OQS60_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS2385987.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="OQS60_000113"
     CDS             23801..24874
                     /locus_tag="OQS60_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS2385988.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25001..28447
                     /gene="mfd"
                     /locus_tag="OQS60_000114"
     CDS             25001..28447
                     /gene="mfd"
                     /locus_tag="OQS60_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS2385989.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28591..29547
                     /gene="ldtC"
                     /locus_tag="OQS60_000115"
                     /pseudo
     CDS             28591..29547
                     /gene="ldtC"
                     /locus_tag="OQS60_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29630..29887)
                     /gene="bhsA"
                     /locus_tag="OQS60_000116"
     CDS             complement(29630..29887)
                     /gene="bhsA"
                     /locus_tag="OQS60_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS2385990.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30128..30760
                     /gene="comR"
                     /locus_tag="OQS60_000117"
     CDS             30128..30760
                     /gene="comR"
                     /locus_tag="OQS60_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS2385991.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30822..31361)
                     /locus_tag="OQS60_000118"
     CDS             complement(30822..31361)
                     /locus_tag="OQS60_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS2385992.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31588..32892)
                     /gene="ndh"
                     /locus_tag="OQS60_000119"
     CDS             complement(31588..32892)
                     /gene="ndh"
                     /locus_tag="OQS60_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS2385993.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33300..33842)
                     /locus_tag="OQS60_000120"
     CDS             complement(33300..33842)
                     /locus_tag="OQS60_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2385994.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33865..34890)
                     /gene="nagZ"
                     /locus_tag="OQS60_000121"
     CDS             complement(33865..34890)
                     /gene="nagZ"
                     /locus_tag="OQS60_000121"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS2385995.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34901..35725)
                     /gene="thiK"
                     /locus_tag="OQS60_000122"
     CDS             complement(34901..35725)
                     /gene="thiK"
                     /locus_tag="OQS60_000122"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS2385996.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35706..36347)
                     /gene="lpoB"
                     /locus_tag="OQS60_000123"
     CDS             complement(35706..36347)
                     /gene="lpoB"
                     /locus_tag="OQS60_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS2385997.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36361..36738)
                     /locus_tag="OQS60_000124"
     CDS             complement(36361..36738)
                     /locus_tag="OQS60_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS2385998.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36741..37100)
                     /gene="hinT"
                     /locus_tag="OQS60_000125"
     CDS             complement(36741..37100)
                     /gene="hinT"
                     /locus_tag="OQS60_000125"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS2385999.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37434..39623
                     /gene="fhuE"
                     /locus_tag="OQS60_000126"
                     /pseudo
     CDS             37434..39623
                     /gene="fhuE"
                     /locus_tag="OQS60_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39683..41116)
                     /gene="ptsG"
                     /locus_tag="OQS60_000127"
     CDS             complement(39683..41116)
                     /gene="ptsG"
                     /locus_tag="OQS60_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS2386000.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41411..42208)
                     /locus_tag="OQS60_000128"
     CDS             complement(41411..42208)
                     /locus_tag="OQS60_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS2386001.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42219..43223)
                     /gene="holB"
                     /locus_tag="OQS60_000129"
     CDS             complement(42219..43223)
                     /gene="holB"
                     /locus_tag="OQS60_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS2386002.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43220..43861)
                     /locus_tag="OQS60_000130"
     CDS             complement(43220..43861)
                     /locus_tag="OQS60_000130"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS2386003.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43851..44873)
                     /gene="yceG"
                     /locus_tag="OQS60_000131"
     CDS             complement(43851..44873)
                     /gene="yceG"
                     /locus_tag="OQS60_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS2386004.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44876..45685)
                     /gene="pabC"
                     /locus_tag="OQS60_000132"
     CDS             complement(44876..45685)
                     /gene="pabC"
                     /locus_tag="OQS60_000132"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS2386005.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45805..47046)
                     /gene="fabF"
                     /locus_tag="OQS60_000133"
     CDS             complement(45805..47046)
                     /gene="fabF"
                     /locus_tag="OQS60_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS2386006.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47134..47370)
                     /gene="acpP"
                     /locus_tag="OQS60_000134"
     CDS             complement(47134..47370)
                     /gene="acpP"
                     /locus_tag="OQS60_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS2386007.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47581..48315)
                     /gene="fabG"
                     /locus_tag="OQS60_000135"
     CDS             complement(47581..48315)
                     /gene="fabG"
                     /locus_tag="OQS60_000135"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS2386008.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48328..49257)
                     /gene="fabD"
                     /locus_tag="OQS60_000136"
     CDS             complement(48328..49257)
                     /gene="fabD"
                     /locus_tag="OQS60_000136"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS2386009.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49273..50226)
                     /gene="fabH"
                     /locus_tag="OQS60_000137"
     CDS             complement(49273..50226)
                     /gene="fabH"
                     /locus_tag="OQS60_000137"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS2386010.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50294..51364)
                     /gene="plsX"
                     /locus_tag="OQS60_000138"
     CDS             complement(50294..51364)
                     /gene="plsX"
                     /locus_tag="OQS60_000138"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS2386011.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51445..51618)
                     /gene="rpmF"
                     /locus_tag="OQS60_000139"
     CDS             complement(51445..51618)
                     /gene="rpmF"
                     /locus_tag="OQS60_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS2386012.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51670..52191)
                     /gene="yceD"
                     /locus_tag="OQS60_000140"
     CDS             complement(51670..52191)
                     /gene="yceD"
                     /locus_tag="OQS60_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS2386013.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52390..52974
                     /gene="yceF"
                     /locus_tag="OQS60_000141"
     CDS             52390..52974
                     /gene="yceF"
                     /locus_tag="OQS60_000141"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS2386014.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53086..54045)
                     /gene="rluC"
                     /locus_tag="OQS60_000142"
     CDS             complement(53086..54045)
                     /gene="rluC"
                     /locus_tag="OQS60_000142"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS2386015.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54618..57803
                     /gene="rne"
                     /locus_tag="OQS60_000143"
     CDS             54618..57803
                     /gene="rne"
                     /locus_tag="OQS60_000143"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS2386016.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57998..58951)
                     /gene="flgL"
                     /locus_tag="OQS60_000144"
     CDS             complement(57998..58951)
                     /gene="flgL"
                     /locus_tag="OQS60_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS2386017.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58963..60606)
                     /gene="flgK"
                     /locus_tag="OQS60_000145"
     CDS             complement(58963..60606)
                     /gene="flgK"
                     /locus_tag="OQS60_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS2386018.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60672..61613)
                     /gene="flgJ"
                     /locus_tag="OQS60_000146"
     CDS             complement(60672..61613)
                     /gene="flgJ"
                     /locus_tag="OQS60_000146"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS2386019.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61613..62710)
                     /gene="flgI"
                     /locus_tag="OQS60_000147"
     CDS             complement(61613..62710)
                     /gene="flgI"
                     /locus_tag="OQS60_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS2386020.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62722..63420)
                     /gene="flgH"
                     /locus_tag="OQS60_000148"
     CDS             complement(62722..63420)
                     /gene="flgH"
                     /locus_tag="OQS60_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS2386021.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63473..64245)
                     /gene="flgG"
                     /locus_tag="OQS60_000149"
                     /pseudo
     CDS             complement(63473..64245)
                     /gene="flgG"
                     /locus_tag="OQS60_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64417..65172)
                     /gene="flgF"
                     /locus_tag="OQS60_000150"
     CDS             complement(64417..65172)
                     /gene="flgF"
                     /locus_tag="OQS60_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS2386022.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65192..66397)
                     /gene="flgE"
                     /locus_tag="OQS60_000151"
     CDS             complement(65192..66397)
                     /gene="flgE"
                     /locus_tag="OQS60_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS2386023.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66422..67117)
                     /gene="flgD"
                     /locus_tag="OQS60_000152"
     CDS             complement(66422..67117)
                     /gene="flgD"
                     /locus_tag="OQS60_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS2386024.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67129..67533)
                     /gene="flgC"
                     /locus_tag="OQS60_000153"
     CDS             complement(67129..67533)
                     /gene="flgC"
                     /locus_tag="OQS60_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS2386025.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67537..67953)
                     /gene="flgB"
                     /locus_tag="OQS60_000154"
     CDS             complement(67537..67953)
                     /gene="flgB"
                     /locus_tag="OQS60_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS2386026.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68108..68767
                     /gene="flgA"
                     /locus_tag="OQS60_000155"
     CDS             68108..68767
                     /gene="flgA"
                     /locus_tag="OQS60_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS2386027.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68843..69136
                     /gene="flgM"
                     /locus_tag="OQS60_000156"
     CDS             68843..69136
                     /gene="flgM"
                     /locus_tag="OQS60_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS2386028.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69141..69557
                     /gene="flgN"
                     /locus_tag="OQS60_000157"
     CDS             69141..69557
                     /gene="flgN"
                     /locus_tag="OQS60_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS2386029.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69597..71132)
                     /gene="murJ"
                     /locus_tag="OQS60_000158"
     CDS             complement(69597..71132)
                     /gene="murJ"
                     /locus_tag="OQS60_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS2386030.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71242..72165)
                     /locus_tag="OQS60_000159"
     CDS             complement(71242..72165)
                     /locus_tag="OQS60_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2386031.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72167..72814)
                     /locus_tag="OQS60_000160"
     CDS             complement(72167..72814)
                     /locus_tag="OQS60_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS2386032.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72825..73409)
                     /gene="rimJ"
                     /locus_tag="OQS60_000161"
     CDS             complement(72825..73409)
                     /gene="rimJ"
                     /locus_tag="OQS60_000161"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS2386033.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73645..74853
                     /gene="mdtH"
                     /locus_tag="OQS60_000162"
     CDS             73645..74853
                     /gene="mdtH"
                     /locus_tag="OQS60_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS2386034.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74917..75564
                     /gene="grxB"
                     /locus_tag="OQS60_000163"
     CDS             74917..75564
                     /gene="grxB"
                     /locus_tag="OQS60_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS2386035.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75698..76258
                     /locus_tag="OQS60_000164"
     CDS             75698..76258
                     /locus_tag="OQS60_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2386036.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76364..77410
                     /gene="pyrC"
                     /locus_tag="OQS60_000165"
     CDS             76364..77410
                     /gene="pyrC"
                     /locus_tag="OQS60_000165"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS2386037.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77484..77729
                     /gene="dinI"
                     /locus_tag="OQS60_000166"
     CDS             77484..77729
                     /gene="dinI"
                     /locus_tag="OQS60_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS2386038.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78019..78273
                     /gene="bssS"
                     /locus_tag="OQS60_000167"
     CDS             78019..78273
                     /gene="bssS"
                     /locus_tag="OQS60_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS2386039.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78388..79506
                     /gene="solA"
                     /locus_tag="OQS60_000168"
     CDS             78388..79506
                     /gene="solA"
                     /locus_tag="OQS60_000168"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS2386040.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19597 a        20911 c        19710 g        19360 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccaac gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    25021 tacactgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgcagcctg
    25081 tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac tcattgcacc
    25141 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg atcagatggt
    25201 gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc atcaggacat
    25261 tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg gcgtactgat
    25321 tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc acggtcatgc
    25381 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    25441 cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca cgcgcggcgc
    25501 gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt tctttgatga
    25561 tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg aggaagtaga
    25621 agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    25681 ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttaccagca
    25741 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt tcttcagcga
    25801 accactgccg ccgctgttca gttatttccc tgccaatacc ttgctcgtga atactggcga
    25861 tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg agaatcgcgg
    25921 cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg tggacgagct
    25981 cttctcagag ctgaaaaact ggccccgggt gcagctaaaa actgaacatt taccgacaaa
    26041 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    26101 aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc cggtggtgtt
    26161 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac gaattaaaat
    26221 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atctgatgat
    26281 tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga tctgcgaaag
    26341 cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca ccatcaaccc
    26401 cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg tccatctgga
    26461 gcacggtgtc gggcgctatg ccggaatgac cacgctcgaa gcgggcggca ttactggcga
    26521 gtatttgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    26581 tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    26641 cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcggcgga
    26701 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    26761 tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcgca
    26821 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    26881 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    26941 taaccacaag caggtggcgg tgttggtgcc taccaccctt ctcgcgcagc agcattacga
    27001 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    27061 cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa tcgatattct
    27121 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    27181 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    27241 gaacgtggat attctgacgc ttactgcaac gccgatccca cgcacgctga atatggcaat
    27301 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    27361 aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc gtgaaatttt
    27421 gcgcgggggg caggtttatt atctctacaa tgatgtggaa aacatccaga aagctgccga
    27481 acggctggca gaactggtgc cagaagcgcg gatcgccatc ggtcacgggc agatgcgcga
    27541 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    27601 tacaaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta tcattgaacg
    27661 cgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg gacgttcgca
    27721 tcatcaggca tatgcatggt tgctgacacc gcatccaaaa gcgatgacta ccgatgcaca
    27781 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    27841 gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa gcggctcaat
    27901 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg atgcactgaa
    27961 agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag tcgagctgcg
    28021 gatgccgtcg ctattgccag atgatttcat tcctgacgtg aatacgcgtt tgtcgttcta
    28081 taaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    28141 cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg cccgtctgcg
    28201 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggggtgat
    28261 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    28321 gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg atttgagtga
    28381 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    28441 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    28501 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    28561 caataacctt atatttatta ttgatttttg gtgatgatca aaacgcgttt ttctcgctgg
    28621 ctaacgtttt ttacgttcgc cgctgccgtg gcgctaccgg caaaagccaa cacctagccg
    28681 ctgccgccag cgagcagtcg tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac
    28741 ggtggttctc tggaagccat cgccaaaaaa tataacgtcg gctttctcgc tctgttacag
    28801 gctaaccccg gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg
    28861 caaaccctac ttccagacgc gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt
    28921 ctctattact acccgccggg taaaaattcg gtaaccgtgt atccaatagg tattggtcag
    28981 ttaggtggtg acacgctgac accgacaatg gtgaccaccg tttcagacaa acgtgcaaac
    29041 ccaacctgga cgccaacggc aaacatccgc gcccgttata aagcacaggg aattgagttg
    29101 cctacggtag tgccggctgg accggataac ccaatgggcc atcatgcgat tcgtctggcg
    29161 gcctatggcg gcgtttattt gcttcacggt acgaacgccg atttcggcat tggcatgcgg
    29221 gtaagttctg gctgtattcg tctgcgggat gacgatatca aaacactctt tagccaggtc
    29281 accccaggca ccaaagtgaa tatcatcaac actccgataa aagtctctgc cgaaccaaac
    29341 ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga agattgatga cgatccgcag
    29401 ctgctgccaa ttacgctgaa tagcgcaatg caatcattta aagatgcagc acaaactgac
    29461 gctgaagtga tgcaacatgt gatggatgtc cgttccggga tgccggtgga tgtccgccgt
    29521 catcaagtga gcccacaaac tctgtaaatc ctggataaaa aaaatccccg cgacacggca
    29581 tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag ggttaatgct tatttataaa
    29641 ttactgctgt tccatggagg gtattcggac cggttacaga agtaatacgg aaagattttg
    29701 cgcccatctc atccgctttt tgcgccagct gctcttccag cgatcccaga tttgtccccg
    29761 cgttagcact gatagtaccg actttttgtt ggccttctgg cgttgactga acttcgacag
    29821 ccgcaaagtt ggcaaatgac atggagctta aaatcgccgc agcgatgagg gtttttacgt
    29881 ttttcataat agtggcctta tgcagatgaa tgacgtcgca agaaattact taacgatcga
    29941 taactaaatg atagatgtga tctggatcac atacaagata ttttttataa caatcattaa
    30001 ttaatttaaa aatctttaaa tttcagttaa atagaaagca attattttta tgggatctgg
    30061 actggtgaac ggaggtgatt atttttataa taacctttca ttcacagtca ccaggttcaa
    30121 cggcatcatg gcaactgact caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt
    30181 gttcgacagg gatgccgcgc ttgataaggc catgaaattg ttctggcaac acggttatga
    30241 agcgacttct cttgcggacc tcgtcgaagc gaccggagcc aaagcgccca cgctatacgc
    30301 ggaatttacc aacaaagagg ggttatttcg tgccgttctc gaccgctata tcgatcgttt
    30361 tgccgctaag catgaagcac agctgttttg tgaagagaaa agcgtggagt ctgcgctggc
    30421 tgactatttt gctgccatcg ccaactgctt taccagcaaa gacaccccgg caggctgctt
    30481 catgatcaac aactgcacca ccctctcccc agattcagga gatatcgcca atacgttgaa
    30541 atcacgccat gcgatgcaag agcgcacttt gcagcagttt ttatgtcaac gacaagcgcg
    30601 cggggaaatc ccgacccact gtgacgtgac acatctggca gaattcctta attgtattat
    30661 tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg gaaaaactga tgcagattgc
    30721 cggaacgact ttgcgtttat ggcccgaact ggtgaaataa gtcgcaaaaa aagcccctca
    30781 gcctgagcga atgaggggcc aaattgcaga gtacagcctt gttatacttt gttattcaaa
    30841 atcagttgcc cgttactatc tagcgggatc tgcgtacccg gatcgcggtc catgcggatt
    30901 ttgccctgct gatcgccaat cttataggtc acatcataac cgagcatttt ttctgacttg
    30961 tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag tatcgctttc ctggagagag
    31021 ccctggatct ggttaccggc atatccacca cccagcgccc ccacaacagt ggcgacatct
    31081 ttaccacgac caccaccaaa ctgatgccct atcacgccgc cagcaacagc gccgagcacc
    31141 gacccggtaa tgcgattttc atcctgcacc ggtcgacgat gggtcactgt gacgttgcga
    31201 cactcctgac gcggtgtttt aaccgtttcc ttgattgggg ttgcagaaac aacctgagcg
    31261 tattgcgggc cccgttcaaa cacgttcaga ctggccactg ccgctacgcc cagcgcagct
    31321 gcgacaccaa tcccgatacc cgccaacatt gatttattca cgggaacacc tccttctttg
    31381 cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta tcagacagaa gatgaaaagc
    31441 gcgggaatgg ctggaaatac ttaaggattc ggagaagtct gaaggaatgc ctgatgcgac
    31501 gcttaacgcg tcttatcagg cctaccgctc gtaccatttg tccagggcgg atgaggcgtt
    31561 tatgccacat ccgccagtgt acgtcgatta atgcaacttc aaacgcggac ggataacgcg
    31621 gttaatactc cccaccagca tcattaatcc ggttttaaag taaccatgca gcgcaatctg
    31681 atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt cgtccttcaa tcatcattga
    31741 gccgcgcgtc aggttaccca tcaggctacc gacagtggag aagttcgaca gcgataccag
    31801 cgaaccatga tctttatact gataattttt cagcggttta ccgttcatct gcgccagaat
    31861 atttttcatt gcgcaggtcg ccatctggtg tgcagcctga gcacgcggcg gaacaaagcc
    31921 cccttccgga cgcgggcatg acgcgcagtc gccaatagcg taaatgtctg gatcgcgggt
    31981 ggtttgcagc gtcggttcca ccaccagctg gttgatacgg ttggtttcaa gaccaccgat
    32041 atctttcagg aagtctggcg ctttgatccc ggctgcccac accatcagat cagcctcaat
    32101 atattcacca tctttagtgt gcaggccgcc ttcatcagca ctggtgacca tggtttgcgt
    32161 cagcacgcga acgccaagtt tcgttagctc gttgtgggcc gcagcagaga tacgtggcgg
    32221 taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt acgttcaggg cttcgttggt
    32281 caggcctttg taaccgtagc tgtgcagttg cttgaccgca ttgtacaatt cagcggagag
    32341 ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc actttaccat tcgcgcccag
    32401 gttggcggag tatttcagga acagattcag catctcctgg tggaagcgac gcgcctggtg
    32461 cgggttatcg aggaaaatgc agttctcttt gacacctggc gtattgaaat cgttagaggt
    32521 gctacccagc gccattacca gggtgtcata ggcgatttta cgttccggaa ccagcagttc
    32581 acctttctca tcgcgcagtt ctgcaatagt gattgttttc gcttcacgat caatatcaat
    32641 gacggaaccc agctggaact ggaaaccatg attgcgcgca tgggccagat agctcaacgc
    32701 atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc agcagcggtt tccacagatg
    32761 gctgtggtta cgatcgacca gcgtaatttt ggcttttttc ttgcgtccca gcttatgccc
    32821 cagctgtgtt gccatttcca gcccaccagc accgccgccg acaatcacaa tttttttcaa
    32881 tggcgtagtc aacgtgaccc ccttaaattt attaaccaat tgttaattaa tggttataaa
    32941 catagctctt aattaacaac aggttacagc aatgaaaatg ttcttcgcta ttgagaataa
    33001 catgaatggt gcattggtca taccaaaatt gatgttaatc aagttttgtt gctgaaaaga
    33061 taggcgactt taccgggtga gatcaaaaaa cagagtttat tgtttattat ttgagcaagt
    33121 ccggaagggt gatatagggt gacgcgtata tcgagggcgt ttggtcgtcc tgataagatg
    33181 cgcagcatcg catcaggcat tgatgctcga ctgtcggatg cggcgtaaac gccttatccg
    33241 acttacagaa ttcggagcgc atcaggtacc ggcaaggtta cctgatgcga cgagggcatt
    33301 tacccgaggg ttttgaacgc cttaatgcgc tgcaaatgcg gggagatatt cttgaatttg
    33361 tgcgtctgct cttcgtccca gacaatctcg taataatgat gcaactcttc agatgtccgc
    33421 tggctgttaa gcgcttcatc attacgcgac aaaatcacca ggcaacgatc gcgattcttc
    33481 tcgcggaagt tggtcacaca cttagtggca atatcggcat actcttccgg gcgatcaatc
    33541 ttgccttcca tgttctcata agggaacaaa ttagggttga agatcacctg gcggatatcg
    33601 caaagaaaac caatccgttc cgcccagtat ccgcccagac caacgccgca aatcagcgga
    33661 cgctcgtcga cgttcagttg caacattttg tccacttctt tgagcagatg ctgcatatca
    33721 tgtttcggat gccgcgtact gtagcttatc aagcgtacat ccgggtcaat aaactgcaat
    33781 tgtaagactt tctcgtggtt acccggactg ttagagtcaa aaccgtgtaa atagataatc
    33841 atcgcttcct cacataagcc agggttagtg acctgctttc tcttcctgcc agcgttcatg
    33901 taactgattc agacgggtgc tgatcgcctt ccagcgagcc gagtccatca gttcctgtcg
    33961 cgaaaatgaa cctttatgat acaaacgtgt aacacgttct gccttgatcg gtgacagatt
    34021 atctaacacg ctgacggccc ctttacgatt attgcagacc aggatcatat cgcaacctgc
    34081 gtccagtgat gcctgcccgc gttcggcata actgcccata atcgcggcac cttccatcga
    34141 taaatcgtca gagaaaatta caccgtcaaa acacagttcc tgacgcaaaa cggttttcag
    34201 ccagtaggaa gaaccgctcg ccggacgcgg atcaacatca ctgtagatca catgcgcagg
    34261 cataatggcg tcgagtttat tttcgcggat taaggaactg aagaccgaca tatctttagc
    34321 gcgaatctcc gcttgtggac gtggatcgca cggtgtttct ttgtgtgagt ctgccgttac
    34381 tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt ccggcttcat gcataccatc
    34441 aataaaccgg ctggcaattg ccagggcttt ttgtggatcg gcatgataag aacgctcgcc
    34501 aatcgccgcg ctgatatgcc cgacatccag cactggcgca aagctgatat caatatccat
    34561 agcgatcatt tcgctggcca tcagccaacc cgcctcttgc gccagtttgc cgccctcttc
    34621 cattcccaac agcgcagcaa aggattgtgc tgccggtaag cgggtaaaac cttcgcgaaa
    34681 acgctgcacg cgtccacctt cctgatctac cgccaccacc agatgattgc gcgatgctgc
    34741 gcggatctgg cgcaccagtt cacgtaactg ggcaggatca tgatagttac gcgtaaagag
    34801 aatcagccct cccaccagcg gatgcgccag tatttcacgc tcttccgcgt ccagttcgta
    34861 accttcgaca tccaacatta ctggacccac actgctctcc ttattgtttt attaatagct
    34921 gccgccaggt gtcatcggcc agcctgataa attgttgatc gccgatttgt cgccagcggt
    34981 actcaaacca ccctgctttg agcatcagca gccagggaaa ccatcgcctg acctgacgcc
    35041 ataattgcgc cggataaatc ttcgcgcgag tggcatagtc attgaccaat tgccggtgct
    35101 gttcagtatt ttccacccac accgccgcca gttccagcgc gatatcacca tctccggcat
    35161 actcccagtc gatgagtttt aaccctgacg cgctatgcac taaatttccg gcgtggacat
    35221 ccatatgcaa tggacttaag cgtaaaggcc gtggttcccg cgctttgcgc agacgtttta
    35281 acattcgcag ccaacccaca gtccgccgcg ccggatcgct ttgctgccag tacagttcca
    35341 gtaagggcaa cagcgttatt cgccagccaa aacgtggttg ttgatgaaga taatacagca
    35401 agcctgccag ttcgttggta tccggcaaat acgtttttac cgcgccgggc agatagtcga
    35461 ctaccatcca gtcacggaga tataaatgcg gcttcggtgc aatgcttgcg ggtagttgtg
    35521 ataaagcccg atactggcgc aagaacgcgg actgcggcgc atcaggatcg tgcggctgac
    35581 gcacaacaaa acgctggccc tgatgttcaa tgagaaaact accgccacta agcccactat
    35641 taaacgtcgt gacgggatga aactgcggga aaaagcgcga cagcaattcg tcgcgcgtga
    35701 tgggattatt gctgcgaaac ggcaccttta cctgaccaga taatttcgcc cgtctgcacc
    35761 agcatcagct gcatttgtag ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc
    35821 acgtaatgag cgccgacatt gcgggcaatg cctatggctt tactacgggt acctaaactg
    35881 tcctgcggcg acaaacctaa ctgttgctta gccatcgaca gctgctgggc ggaaaccagg
    35941 gtaaatttcc cgttattagc cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc
    36001 agcgaaccgt tagtacggtt gttaacgcta tcaaccagca ggacgctacc cgcagtcacc
    36061 ccgtcagccc caagcatctt actgaccatc ggctgcattg cgccattcca gtcataatgg
    36121 cgaatatgcg gcgcaggcgg tgccgtttga tcttcgtgct caattgggcc tggctgctgc
    36181 gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt gtggctcggc tggttgttcc
    36241 ggcgctggtt tcacttcttc taccggtgca ggttcacgtt gccccacaca cccggcgaga
    36301 aacatcgcca gcgcggtaat caaggcgtag cgactcattt ttgtcatcaa gattcacccc
    36361 ttacaaatat agataaagtc tgactttgtg cgcccccaga aaattggcgc tgccgtacag
    36421 cgttaccgcc gaatgtgcgg gaatgctaac gctgcgtggc ctttccagag gatgcatctc
    36481 cagccctctg gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc
    36541 gttataaagc gttgaggatg ctgaaggttg aatatcagac gtcgaaagga cgggcttttc
    36601 tgcactgatg cccgcagcca ataaagatga ctccatcacc agcgattgct catcattcac
    36661 cggaatttcc ggatgtgaac gacagcccac cagcagtaac aacgccagag acaccagccc
    36721 aaagcatcct tttctcatcg ttacagacct ttatgcgcca gcattggtcc cagcggacgg
    36781 ccacccaaca agtgcatatg gatgtggtaa acctcttgtc cgccatggcg gttggtattc
    36841 atgatcagac gatagccatc ttcggcaata ccttcttgct cagcaatttt tgccgctacg
    36901 gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaca cgtcgttcac agtcggaatg
    36961 aggatattcg gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc
    37021 gttaccagat catcctggta gacgatatcg gaggggatct cacgacgaat aattttgctg
    37081 aatatagttt cttctgccac gacattttcc tttttcaaat agccatgcgt tgcgccatgc
    37141 tacgccgcgc cacactgcga gtatagagta tggtcgagtt aaacctgctc tttcaacctt
    37201 aactcacgat ttcttaagca aaaaatcgtt ttgctgccgt atatatcgcc attattccca
    37261 tttctgcctg cgaatgcgct gaaaagacat tctctgtttg gtttacacga caaatatgaa
    37321 tgcgcatatt tctcatttgc atttacaaac aaaattattc gcacactaaa tataaccgct
    37381 catcaccctc ctccggcatg agcctgacga caacataaac caagagattt caaatgcttt
    37441 caacacaatt taacagggat aatcaatatc aagccatcac caaaccgtca ctacttgccg
    37501 gttgcatagc actggcacta ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa
    37561 cggtgattgt tgagggttca gccacagctc cagatgatgg cgaaaatgat tacagcgtaa
    37621 cgtctacctc tgcgggtacc aaaatgcaga tgactcaact tgatattcct cagtcggtca
    37681 ctattgttag ccagcagcgg atggaagatc agcagttaca aacgctgggc gaagtgatgg
    37741 aaaacacgct ggggatcagc aaaagtcagg cggattccga tcgtgctctt tattattccc
    37801 gtggattcca gatcgataac tatatggttg atggtatccc cacctatttt gaatcgcgct
    37861 ggaatctggg cgacgcactt tctgatatgg cactctttga acgcgtagaa gtagtgcgtg
    37921 gcgcgacagg actcatgacc gggacgggta atccatctgc ggcaattaat atggttcgaa
    37981 aacacgcgac cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt agctggaaca
    38041 aagaacggta tgtggcggat ttacaaagcc cactcaccga agacggtaaa atccgcgcgc
    38101 gaattgtcgg cggctaccag aataacgact catggctgga ccgctacaac agtgaaaaga
    38161 ccttcttctc gggcattgtc gatgctgatt taggcgatct tacgacgctt tcagccggtt
    38221 acgaatatca gcgcattgat gttaatagcc caacctgggg cggtttaccg cgctggaata
    38281 ctgatggcag cagcaacagt tacgatcgtg cacgcagtac cgcacctgac tgggcgtaca
    38341 acgataaaga gatcaacaag gtctttatga ccctgaagca gcggtttgct gatacctagc
    38401 aagcgacact gaatgccacc cactctgaag tcgaatttga cagcaaaatg atgtatgtcg
    38461 atgcctatgt aaacaaagcg gatggtatgc tggttgggcc atacagtaat tatggacctg
    38521 gctttgatta tgtcggcggc accggttgga acagtggcaa acgtaaagtt gatgcgctgg
    38581 atttgttcgc tgacggtagt tatgaattgt ttggtcgtca gcacaatcta atgtttggtg
    38641 gcagttacag caaacaaaac aatcgttact tcagttcatg ggccaacatc ttcccggatg
    38701 aaattggcag tttctacaac tttaatggca atttcccaca aaccgactgg tcaccacaga
    38761 gcctggcaca ggacgatacc acacatatga aatcgttata tgctgctact cgagtcaccc
    38821 ttgccgatcc gctgcatctg atcctcggcg cacgttatac caactggcgg gttgatacgc
    38881 tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg tttgacatca
    38941 atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa aatgatcgtg
    39001 acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg ggtctgaaat
    39061 cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt gagcaggata
    39121 atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc gcctataaag
    39181 cggtggatgg gacagtcagt aaaggggtgg aatttgaact caacggcgca attaccgaca
    39241 actggcagct gacatttggc gcaacgcgct atattgcaga ggataacgaa ggaaacgccg
    39301 ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg ttgcctgtca
    39361 tgccagagtt gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg
    39421 tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg gatctcttca
    39481 cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac ctgttcgaca
    39541 aaacctacga taccaacatg gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca
    39601 ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc tggcggcctt
    39661 agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc tcggttttca
    39721 ggttatcgga tttagtaccg aaaatcgcct gaacaccaga accagcaacc actacgcccg
    39781 ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca acgctgacgc
    39841 gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca aatgcagcaa
    39901 ccagagccgg tgccatttcg ctggtacctg ttgcttttgc atcttcagtc gcgtcttcac
    39961 gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa
    40021 tcgcataacc gataccgacg atcgggaaca gccacagttt gctgctgtta ccagacagaa
    40081 cgataaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc agaagaatac
    40141 agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc ggcgcaacga
    40201 acatgaagga gaactcgatc ggctcggtga taccggtcag gaacgaggtc agcgccgcgg
    40261 agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc cagatagcaa
    40321 ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt ttacccgcag
    40381 tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg
    40441 tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga ccaaacggca
    40501 ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc tggtaagcag
    40561 cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag gacagcacaa
    40621 cgccagtaaa gatggcagcc aggccagaaa tgatcggcac aaagcgttta ccggcaaaga
    40681 agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac gctgcgatcg
    40741 caccggagat aatccctccg agtacgccag tatccgccag gtgtttagag gcgatttctt
    40801 cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc atgatgccat
    40861 aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg agggcgacac
    40921 cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct gccataacat
    40981 gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc agaatacctg
    41041 cgataggcag tacggatacc ggcagcatca gcgatttacc gaccttttgc aggttagcaa
    41101 atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt tacgttctca
    41161 cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc tttatttatt
    41221 acacagagta aaataattca gtgccgattt gtttgacggc tatcacattt caacttcaga
    41281 tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa acgtggttat
    41341 tcaccgccca attttcggcg gtgaacttta ctcgttttag ccattaatta cgagctttaa
    41401 aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac agacgggcga
    41461 agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac acagccatgt
    41521 attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc ggtaccggcg
    41581 caaggtaagg tgagtcagtt tccaccagta accgatccag ggggacataa cgcgcagcat
    41641 ctcgaagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg taaaatccga
    41701 gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt acgccaccgc
    41761 aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcgcgggcg tcacgggtat
    41821 ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg aaggactctt
    41881 gctgacgaac tttagtttcc ggtgtgtaat aataatccag cccggtttca cccagcgcta
    41941 caacaccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga tcattctggt
    42001 tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc agatcccgca
    42061 tatgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg
    42121 cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc agaccatcga
    42181 gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg aaccggtagc
    42241 acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat gagaagctcg
    42301 cggttgatgc ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc ccccagtata
    42361 gcctgcaggc gcgagggaga aagatggttt gccagttcga cgacaaggcc cggcacatca
    42421 acattggtca cctgcgcagc accatgatgg cgttttagcg catccatcag caacgttgcc
    42481 agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgccagcag cgaataccag
    42541 tcgcccgatg gcacgctata tgccaatgcc tgacacaatg tttcacgagc ctgccagtta
    42601 tctccctgaa acaacgccag tgccgcgcca ggcgaaccgg cgcttaagcg caatgcggca
    42661 agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc
    42721 ggcggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag taaacgttca
    42781 ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg gctcttcaag cgttttcagc
    42841 aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac aactttcgca
    42901 ccacctaggc gtgcgtgctc attcagcttt tcggtgacct cacgtactgc atcaatgccc
    42961 agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt gccagcctgc
    43021 atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt
    43081 aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa cgcctgaatg
    43141 agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc gaaatcaggt
    43201 cgtaaccatg gataccatct catgcgtcca actccttcac ccagtgggtc acggtagtgc
    43261 ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga atacttttat
    43321 cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt
    43381 gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa
    43441 catcgagata gagcgttaag tcggggcgaa aatccccgag aacagcatca cgcagtgttg
    43501 ccagcatatg ttggtcaata ccacgtcctc cgccctgata cgcctgagtg gagagatcgt
    43561 ggcgatcgcc aatcacccag gtgccgttag ccagagctgg tttgatgacc gtttctacca
    43621 gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg acttcatcgc
    43681 ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc gtaccgccag
    43741 gttcccgagt gaaaaccatg tcgcggatac ccagttgctc aagcgtctca accaccacat
    43801 tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac ttactgcgca
    43861 tttttttcct taagcacttt cagataatcc tgcacagact tgttatggct ggcaagattg
    43921 gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg cgtttttgcc
    43981 ggatgcgcag cagccttcag cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa
    44041 ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg
    44101 ccattataac gctctcccat cccgtaaatc acggttgggt cggtctgcag gcgcatacca
    44161 atgcgtaaac ggttgataaa taccgaggca acctgatcgc gttcactggc aacggcggtt
    44221 tctttttcga taattgatgc catcgtcacc agctgatttt tatctttata aggcagaccg
    44281 tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg cgctcgcttg
    44341 agtaacgcga catcggtggt attggcggta tacatccagg tgtctggcca gaaccaacct
    44401 tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta cttatcgtca
    44461 ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc gctcagacgc
    44521 atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccag caatttcagc
    44581 atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga
    44641 tccggttcga tacgcagcaa ccattgaaaa acccgtgggc gattgatgat cttatcggca
    44701 taaagctgtt caccaagcgc cagacgtccg gtccctggct tcagggtaaa tatcgtctct
    44761 tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc cacaccagcg
    44821 gcgataccca gtaccaccag caataacaag ataatcaata acactttttt catgactaat
    44881 tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa aaggagacat
    44941 cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc atctcatctg
    45001 cctgcaacgc ctcttccaga gaggcttgca cttcgacaag ctgataagag gattgtgcca
    45061 gcaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc agacgcggcg
    45121 tataaactac attgcccttc cgccagaaca aattagccgc acagcattcc gtaacccacc
    45181 cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga gagcggatca
    45241 atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg cgccccagcc
    45301 gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa tgtgcaggat
    45361 aaaccgtaac ggagagaatc cgcgttgccg gtccactgtt caatgtgctg taccctcgcc
    45421 cgccactacc gcgactgatc acgactttca gcacaccatt ttgctgttct gctgccagcg
    45481 ttttcatctc ttgttcaagc tgaggccaga agtcacagaa aatcatcaac cgctgacaag
    45541 catcctgtag tcgctggata tgcgccgata acaaactgac tttaccgtcg ataactctgg
    45601 cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc agcgattcct
    45661 gcttatgacc gttaattaag aacataccgg ctccttatca gagaattgtt agtgtggcag
    45721 catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc aagcggacct
    45781 tttatatggg tgggaaatga caacttagat ctttttaaag atcaaagaac cattagtgcc
    45841 accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac gtgcttcatg
    45901 cggtacgaaa tccagatcgc aaccttcatc cgggttatcc aggttgatgg ttggcggaac
    45961 agcctgatcg cgcagcgcca ggatggagta gatagattct actgcacccg ccgcacctaa
    46021 caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag cttcaccgaa
    46081 gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc
    46141 gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat ttgccatcgc
    46201 cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat cgctgctcat
    46261 accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc
    46321 ttcaagtacc agcataccgg caccatcgcc cagtacgaag ccatcacgct ctttatccca
    46381 cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa
    46441 accaccaaca cccagcggcg tactggcttt ctctgcgcca cctgcaacca tcacgtcagc
    46501 atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg aagtacaggc
    46561 ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat gacctgccac
    46621 catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac caccgttcat
    46681 cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc caattgcggc
    46741 accaatgcgg gttgcgttct cttccgttat atcaaggcca gaatcctgca tggcctgaac
    46801 gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt tgcgcgagat
    46861 aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat aggcgctagt
    46921 atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt tccaggtaga
    46981 ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta cacgacgctt
    47041 agacacgttt gtcctccagg gagggaaaaa atgattctag tgggacaaaa agataaaact
    47101 caggcggtcg aacgaccgcc tggagatgtt cacttacgcc tggtggccgt tgatgtaatc
    47161 aatggcagcc tgaacggtgg tgattttctc agcttcttcg tccggaatct cagtatcaaa
    47221 ctcttcttcc agagccatta ccagctcaac ggtgtcaaga gaatccgcgc ccaggtcttc
    47281 aacgaaagaa gcattgttgg taacttcttc ctgcttaacg cccagctgtt cgccgataat
    47341 tttcttaacg cgttcttcga tagtgctcat actcttaaat ttcctatcaa aactcgcttt
    47401 cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc
    47461 ttaccacgat tttacgttat tttgaggcct taccccccaa taacgcaaat cattttgcac
    47521 taattattac tcataaccac gcagactgcg cgcaacatga gcattttgtg caaatcgcgg
    47581 tcagaccatg tacatcccgc cgttcacatg caaagtttca cccgtgatgt aagctgcttc
    47641 gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc
    47701 aggaacctgc gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt
    47761 ttcaataaag cccggagcaa caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc
    47821 cagtgattta ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc
    47881 atttcccatg gtaccaacca cagaaccgat agtgataata cgaccatgac gctttttcat
    47941 catagcgcgc attaccgctt ttgacagacg gaaaacagat gaaaggttgg tttcgataat
    48001 atcgttccac tcttcatctt tcattcgcat taacaggtta tcacgagtga taccggcatt
    48061 attgaccagg atatccactt caccaaattc tgcgcgaatt ttttccagaa cagattcgat
    48121 agatgccggg tcggtcacat tcaacatcag acctttgccg ttggcaccta aataatcgct
    48181 gatcgcctga gcgccatttt cactggtcgc agtgccaata actttcgcgc cacgggctgc
    48241 gagcgtttca gcaattgcgc ggccaattcc gcggcttgca ccggttacca gtgcgatttt
    48301 tccttcaaaa ttcatgattt tcctctttta aagctcaagc gccgctgcca tcgctgaagg
    48361 ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac
    48421 cttgcccggg ccgacttcat agagatgttc tacgccttgc gctgccatgt actcaacaga
    48481 cttcgtccac tgaaccgggt tatacaactg acgtaccagt gcgtcacgga tggcatcacc
    48541 attggtttcg catttcacat caacgttatt cacaacagaa actgttggtg cgttaaaggt
    48601 gattttcgct aattctactg ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga
    48661 cggtacgctc actggtaacg gcagagcacg ttttgcgccc gcagctttac aggcagcgcc
    48721 agcacgctca accgcttctt tatgaccggc aataaccacc tgtcccggag agttaaagtt
    48781 taccggagaa acgacctgac ctgctgcagc ttcttcacac gctttcgcaa tagacgcatc
    48841 atccagaccg atgattgccg ccatagcgcc cgtaccttcc ggtacggctt cttgcatgag
    48901 cttgccgcgc atctcaacca gacgcaccgc atcagcgaaa tcaatcacac cagcgcaaac
    48961 cagcgcggaa tattccccca ggctgtgacc ggccatcatt gccggtgctt taccgccctg
    49021 ctgctgccat acgcggtaca gcgcaacaga ggcagtcaac agcgctggct gggtttgcca
    49081 ggttttattc agttcttcag ctggcccctg ctgggtcagc gcccacaggt cgtagcccag
    49141 cgccgcagaa gcttcagcaa acgtttcttc gacaattgga tagctcgccg ccatatcagc
    49201 cagcattcca acggtttgag aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt
    49261 ttaatcctta tcctagaaac gaaccagcgc ggagccccag gtgaatccac cgccaaaggc
    49321 ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg cgtacagctt catccagcgc
    49381 gcacgggaca gaggccgcag aggtattacc gtggcgatcc agcgtcacca cgacattgtc
    49441 catagacata ccgagttttt tcgccgttgc actgataata cgcaggttag cctgatgcgg
    49501 aaccagccag tccagttgag aacggtcaag attatttgcc gccagcgtct catcaacgat
    49561 gtgcgccagt tccgttaccg caaccttgaa gacttcgttg cccgccatcg tcagatgaat
    49621 tgaattctct ggattcacac ggtcagcatt aggcagcgtc agcaactcac catagctacc
    49681 gtcggcatgc agatgggtgg agatgattcc cggctcttca gaggcagcca gcaccgcagc
    49741 gcccgcgcca tcgccaaaaa taataatagt cccacgatcg gttggatcgc aggtgcgcgc
    49801 cagtacatcg gaaccgacga ccagagcata cttcaccgcc ccagatttca cgtattgatc
    49861 ggctacgctt aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca
    49921 acctttaatg cccagcatgc tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc
    49981 agaagtcgtt gccacaacga tcaggccaat ctggtctttc tcaatgcccg ccatctcaat
    50041 tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg
    50101 gcgttcgcgg ataccggtac gagtgacaat ccactcgtca gaggtgtcca ccattttttc
    50161 caaatcggcg tttgtccgca cttgttcggg cagatagctg ccagtaccaa taatcttcgt
    50221 atacatgtac gctcagtcac ttttcggtta tataccgtca cttgcaaact gcgagttcgc
    50281 tggcagcgtc ctgctaccgc agagttccgc ttttgccacc gtccagcagc tcaaaaccag
    50341 ctgggtatac agattccagg cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct
    50401 gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca tgacttttta
    50461 tcaccgtgcc gcgcaatcct aacagacagg cgccgttata ctggtcgggg ttgaggtgac
    50521 tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa taacagtagc caccacgacc
    50581 gttttttccc ttcaccctga gatttcagca gagaaaggaa catcctgaca acaccttcca
    50641 tcgtctttaa tgtgacattt cctgtaaagc cgtcacaaac cagcacatct gtcttgccag
    50701 ttaacaactc attggcttca agatagccga tataattgat agaagggatt gttttaagca
    50761 ccgctgaggc atcccgaata ctgtcgagac cctttacttc ttcttcacca atattgagca
    50821 acgccacgcg aggattggga atttccacca cctcttcagc cagaactgag cccataatgg
    50881 caaattgcac caacattgta ctgtcacaat cgacgttggc ccctaagtca aggaccaccg
    50941 ttttgccctt ttgctgatgt ggtaataccg tcaccagcgc cggacgctca atcccctcca
    51001 ggggcttgag taataatttt gccagcccca tcagtgcccc ggtattaccg gcactgacac
    51061 aggcttgcgc tcgaccttct ttcaccagct ccagggccac gcgcattgaa ctcccacgac
    51121 tggcgcggat agcttgcgaa ggccgggcat cactggcgat aactgactgc gcaggaataa
    51181 tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg
    51241 gattgccgac taaaagaaga gtgagttgcg aattagaatt cagtgcctgc aatgctgcag
    51301 gcactgtcac ggaagggcca aaatcccctc ccatgacatc taacgccagg gttagacgtg
    51361 tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt gcctggggaa aatcctcact
    51421 aagcttcatc acgcagacgc gtgattactt agcgatgacc ttgcggccgc ggtagtaacc
    51481 gtcggcagtg atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta cagacaggct
    51541 ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca cgtttggaac gggttggttt
    51601 attctgttgt acggccatgg accttactcc tcaattacgg tttatccccg atccaacggg
    51661 gagcaccaat tacttacgct ttaagctggc taatacggca aatgggtttg gcttttgcac
    51721 ttcttcaggc agttcaccaa agaccatgtc cgcttcggac acttcacagt gttcagaatc
    51781 atgcaccgga actaccggca aggcgaggat gatttcatct tcaaccattg caagcagatc
    51841 gatttcaccg aattcgttaa cctcaatcgg ttcatacgct tccggcagtg cttcagcctg
    51901 ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag acctgatgag taaacggctt
    51961 cccgcaacgc tgacactcga gcgttaccgt caccttcgca tcgccgttta acactgcgag
    52021 acgttggtta tcgatagcga acgacatgga gcattccaca tcactgtcca cactgactac
    52081 ggattcggcg acgcgctcaa cctgatcagg ggtatagata ccctggtaat caaggcgttt
    52141 ttgagccgta cgaaccggat cgagagtcag gggtaatttt accttttgca tagggcgcgc
    52201 atattaactt tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc
    52261 aataattcgc acacattgcg ggttactgct ttattttcgt tcagtgctga taacgaacat
    52321 caacctgcaa aacgaagcgc atagtttaaa atggctatca tcaatacgct atatctgatg
    52381 gaaaaaaata tgcctaaact tattttagcc tccacctcgc catggcgtcg cgctcttctg
    52441 gaaaaactgc aaatctcttt tgaatgtgca gcaccagagg tcgacgaaac cccacgcagc
    52501 gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca atctctggcg
    52561 tcacgttatc cggatcattt aattattggt tcagaccagg tatgtgttct tgatggcgaa
    52621 atcaccggta aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa agccagcggc
    52681 aatatcgtca ccttctatac tggactggcg ttgtttaatt cggcgaatgg gcatctacaa
    52741 acagaagtgg agccttttga cgtccatttc cgtcacctga gcgaggcgga gattgataat
    52801 tacgtgcgta aggagcatcc cctgcactgc gcgggtagct ttaagagtga aggatttggc
    52861 attacgctgt ttgagcgttt agagggacgt gaccctaaca cgctggttgg tttaccgcta
    52921 atcgcgctgt gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg atagttgttt
    52981 gcgctggatg cggttacgtc gcatccggca atcggtgcag gcggcctgat gcggcgtaaa
    53041 cgccttatca ggccaaccgc ccattatcag gcttatatgc ttgtattagc gcgcgttacg
    53101 cagcttttgc aaacaacgct tcaaaccttc atccatcggc gcttcgatac gcatcacctc
    53161 accggtcccc ggatgggtaa acttcaacgc tgcggcgtgc aggaacagac gatttaatcc
    53221 cgtgcctgct tcagtgagct gtctgtcaaa ttcacggtca ccgtagcgat cgtcaaaggc
    53281 aatcggatga cccgcatact gtgtatgcac acggatctga tgagtacgtc cggttaccgg
    53341 gctacaacgc accagggtgg caaatgcata gcgttcttcc actttaaagc gtgtttccga
    53401 cggtttgcct tcctgactga cacgcacgat acgttcgccg ctttgcagaa tatttttcaa
    53461 taacggcgct tgaacgctct tcacatgcga ctgccactga ccgcgcacca gcgccaggta
    53521 atctttttgc attccttttt cacgtaattg ctcatgcaga gaacgcaacg ccgagcgttt
    53581 tttcgctatc agcaacacac ctgaagtgtc ccggtcgaga cgatgaacca gttcaaggaa
    53641 ccgcgcttcc gggcgcaacg cccgcaaacc ttcgataacg ccgaagctta aaccactgcc
    53701 gccatgtacc gccgtaccgg aaggtttatt cagcaccagg atatgatcat cttcatataa
    53761 gatgacgtcc gccagcgccg ccaccttttg cagatgtggc gaaaccgcct cttcttcccg
    53821 ctcagcaacg cgaaccggtg ggatacgcac ctcatcaccc gcttcgagtt tatattcagg
    53881 cttaatacgt tttttgttca cccgcacttc gcctttacgc aaaatacggt aaatcatact
    53941 ttttggtacg cctttcaatt gggtacgcaa aaagttatcg atacgttgcc ccgcttcgtc
    54001 agcggtgata gcaacaattt ttacggatgg agtctctgtt ttcatggtgg gcgattctaa
    54061 ttagccaaca ggattcgcgc cactcatttt tctatgctta tatttagttt tgcaccttat
    54121 tacttcacta cgtgatcact ttattgatgg ttattaaacc aatcaccagc aagaagtgaa
    54181 aaaactgtga gtaagcgggt gataaatggt aaaagtcatc ttgctataac aaggcttgca
    54241 gtggaataat gaggccgttt ccgtgtccat ccttgttaaa acaagaaatt ttacggaata
    54301 acccattttg cccgaccgat catccacgca gcaatggcgt aagacgtatt gatctttcag
    54361 gcagttagcg ggctgcgggt tgcagtcctt accggtagat ggaaatattt ctggagagta
    54421 atacccagtc tgtttctttg ataattgcgc tgtttttccg catgaaaaac gggcaaccga
    54481 cactctgcgc ctctttgagc tgacgataac cgtgaggttg gcgacgcgac tagacacgag
    54541 gccatcggtt cacacccgga aaggcgttac tttgcccgca gcttagtcgt caatgtaaga
    54601 ataatgagta agttacgatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc
    54661 gcgttgccct tgtagatggg cagcgtcttt atgacctgga tatcgaaagt ccagggcacg
    54721 agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag
    54781 ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc
    54841 gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc
    54901 gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg
    54961 cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgcca aataacccgc
    55021 gcgcaggtgg catttctcgc cgtatcgaag gcgatgaccg taccgaatta aaagaagcac
    55081 tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtaggca
    55141 aatctgctga ggcgctgcaa tgggatttaa gcttccgcct gaaacactgg gaagccatca
    55201 aaaaagccgc tgaaagccgc ccggctccgt tcctgattca tcaggagagc aacgtaatcg
    55261 ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga
    55321 aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca
    55381 aaatcaaact gtataccggc gagatcccgc tgttcagcca ctaccagatt gagtcgcaga
    55441 tcgagtccgc cttccagcgt gaagtccgtc tgccgtccgg tggttcgatt gttatcgaca
    55501 gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata
    55561 tcgaagaaac cgcgtttaac accaacctcg aagctgccga tgagattgct cgtcagctgc
    55621 gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg cctgtacgcc
    55681 accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc
    55741 aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgccagcgc ctgagcccat
    55801 cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg
    55861 acaacgaatc gctgtcgctc tctattctgc gtttgatcga agaagaagcg ctgaaagaga
    55921 acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa
    55981 aacgttctgc ggtgaatgcg attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc
    56041 caaacgatca gatggaaacc ccgcactacc acgtgttgcg cgtgcgcaaa ggggaagaaa
    56101 cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg
    56161 aagaagagtt cgctgaacgt aagcgtcctg aacaacctgc actggcaacc tttgccatgc
    56221 cggatgtgcc gccagcgcca accccagctg aacctgccgc gactgtcgta gccccagcac
    56281 cgaaagctgc aacggcaaca ccagcagctc ctgcacaacc tgggctgtta agccgcttct
    56341 tcggcgcact gaaagcgctg ttcagcggtg gtgaagaagc caaaccgacc gagcaaccaa
    56401 caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaaacct cgtcagagca
    56461 accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa ggcagcgata
    56521 atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg
    56581 agagccgtca gcaggctgag gtaacggaaa aagcacgtac caccgacgag cagcaagcgc
    56641 cgcgtcgtga acgtagccgc cgccgtaacg atgataaacg tcaggcgcaa caagaagcga
    56701 aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc
    56761 cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg
    56821 tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgccgctgcc gaaccaattg
    56881 ttcaggaagc tccagctcca cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc
    56941 aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc
    57001 cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtgag tggtcagcgt cgtcgtcgct
    57061 atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc
    57121 cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac
    57181 aagttgaaga gcagcgcgaa caggaagaag tacaagtgca gccgatggtg actggggtcc
    57241 ctgtcgccgc cgctgtcgaa cctgttgtta gcgcaccggt tgttgaagaa gtggctgaag
    57301 ttgtagaagc ccccgttcag attgccgaac cgcaaccgga agtggttgaa acgacgcatc
    57361 ctgaagtgat tgccgccgca gtaactgaac agccgcaggt gattaccgag tctgatgttg
    57421 ccgtagccca ggaagttgca gaacacgcag aaccggtggt tgaaccgcag gaagagacgg
    57481 cagatattga agaagttgcc gaaactgctg aggttgtggt tgctgaacct gaagttgttg
    57541 ctcaacctgc cgcgccagtc gtcgctgaag tcgcagcaga agttgaaacg gtagctgcgg
    57601 ttgaacctga ggtcaccgtt gagcataacc acgctacggt gccgatgacg cgcgctccgg
    57661 caccggaata tgttccggat gcaccgcgtc acagtgactg gcagcgccct acttttgcct
    57721 tcgaaggtaa aggtgccgca ggtggtcata cggcaacaca tcatgcctct gccgctcctg
    57781 cgcgtccgca acctgttgag taataattag ctcaagtaat caagccctgg taactgccag
    57841 ggctttttta tttcatcttt gaatcaacat attaaaattc atcgaatggc atccttgcta
    57901 accaacaatg caaaataggc aaaaaaatgc ccgccccggc gatcccagag caggcagaca
    57961 aaacataccc agtttcatga tatgttttaa agcgaaatta tttgttgagc tggaagagcg
    58021 acaatccctg catatcggta aatgctttat acgatgcctg caatgccgtt tgctgcatga
    58081 tgtaagatga aatagttgca ttccagtcca catcaaccag atcgctcatc tgctgcgttt
    58141 gccctaaagc gcgatcgcta cctaatgaat ccagcgactc cagttcgttc agctgcgtgc
    58201 ctaattccgc gcgaacggtc agcacattgt tcagcgagtt tttcagtccg cggttggttt
    58261 tatctaacgc cgcagcggcg gtttctttat ccgcttcgct atccgcgacc ggcgttttca
    58321 gggctgcgat ggcgctatcc agcatggcaa aaagattggt ttcagaagcg ctaccgtctg
    58381 gttccgctac cgcgttactg gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca
    58441 ccatcgaacg cgaagcatcg acctgttgct taatactttc tgttccgccg acataatcac
    58501 cgttagcctc actaaacggc gcagtctctg ttttataacc ggcaaaaatg tagcgcccat
    58561 taccgtcagt ggtgttcgcc agattcagca actggtcacg aagcccctga atatccgtag
    58621 ccagcgaggc ccggtcatcg tcactcaagg tgccattgct ggcgtagaca attttttcct
    58681 gagcattctg gatagcagtg gtgacctggc taagtacact ctcttccagt gacacttttt
    58741 gagtggcgaa agtacgcgcc agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa
    58801 caaccgcttg tgatgcagca atgggatcat ctgaagggtt aacgactcgc ttacccgtcg
    58861 acatctgttc gccgtacttc atccattctg cctgagaatt ggtgatacca cgcatgtttt
    58921 gctggtacat catctgggta ctgaaacgca ttttatctcc ccttagcgaa tgttaatcag
    58981 cgcatcaaaa atcgcgtttg ccgtctgcag aacctgcgca ttcgccaggt aatactgctg
    59041 aaaacgttgc agatttccgt actcctcatc gagattgaca ccggaaatcg actgctgctg
    59101 attggaaagc tgcgtcacca cattaccttg cgtggtgcta ctggttttca acgtcgcggt
    59161 tttattaccg atatcactca ctaacgaggc ataagcgtcg ttaaaggatt tcgcaccgcc
    59221 caccgtttta ctattgcttt gcagatccag cagggcctga ccgttgcggt tatcgctatc
    59281 acccgcatct ttttcgctcg ccatcgctat tttcgcttcg tcggtgatta atacatccat
    59341 gttgacgatg gcgtcactta ctggtttcag cgtgaagctg tcgttaacgg caggcgttcc
    59401 tgtaaacgtc aactccagac catcaaatgc cactttaccg ttggcatccg gcgtcaccgt
    59461 aaaagtggta ttgctggcaa ggcgggtgac ctgccactga ttattatcga acgagatttt
    59521 gtaatctgtc gccagtacca cggaggcatc agttaccgtg gcaccgatcg caacgtcacc
    59581 tttgtttttc gtgttttgca gaaccgcggg cttaccgata gtaaagaaat cttcaccagc
    59641 atcgccgtta gcatcaaatc cggctttgtg ttgggtgttg aaagcctcgg caaatgccag
    59701 cgccagttgt ccaagcgtat tacgcgtctg gtccagctcc tgagaacgga atgtcagaat
    59761 gccgcccagc gacccggtat tcagtaattt ctccgggatc tcaatattgc ctgccgtccc
    59821 atcaacataa gcgacagtcg tacgagaagg gtcagcgctg gaaggaactg ccgccagttg
    59881 ccgcgccgta cttccctgaa ccagtgagta accattggcc accgtgatgt tataagtgcc
    59941 gccatcctga acgctgactt ctacaccaac aatctggttt aattcgctca ccagttgatc
    60001 gcgttgatcc agcagattgt taggtgacgc ccctgccccc acgcctgtca ggcgcgagat
    60061 ttgatcgttc aggctggcaa tttgtttagc gtaattgttg atctgatcaa cgctggcacc
    60121 tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat tgatcggtgg ttttaaactg
    60181 attcaccaac ccttctgatt tcccaatcag cgcctggcgc gctgccgggt cttccgcgtt
    60241 actcaccagc gtttgcaggc tggtgaagaa atcctgcatc tgtgttgcca gcgaagaggt
    60301 actggtggag agcatattgt cgattttcga catctgctca tagcgggcgg tcaggccgct
    60361 actttgcgtc tgcgcagcac gtaactggtt ggtaataaac gcatcatact cacgctgcac
    60421 accagaaacg tagacgccat tgccaaccca gccgccagcg cccaacgtgc tattggcctg
    60481 cgccataata gtggtttggc gggtatatcc ggcaacgtta tagctggaga tattattact
    60541 tgccgtattt aacgccgcct gggccgcgtt cagtccgctc atggcgttat tgatcaagct
    60601 ggacatggag gttccttata agccttcaat tacagagtat tatcggcagc gacccgccgg
    60661 acttgagtta ttcagaacag attatcaatg ttcatgctgt aggttttgct caccttgtcg
    60721 cttatcgatt tcatctgctg gatcatgttg gtgagtttac gggcatagtg aggatcggta
    60781 gcataccccg cgtcctgtag ggcctgcgcc ccctgctccg cactcgcggc tgtcgtcacg
    60841 gcggcgtagc gcgggttacg tgttaacagc ccaacgtaat ccgacaatgc ttccagatac
    60901 gagctgtaga cccgaaattt cgcttttact ttcttcgctt cgccattttc atattcagtc
    60961 gtggtgattt cagtaactgg acctttccag ttgccagagg ctttgacgcc aaacaggtta
    61021 tagctcggct cgccgttttc gcggcggatt tgccgttgcc cccaaccaga ctccagcgcc
    61081 gcctgagcga ggatcaaatg atgtggcaca ccgctttgct ggcttgccag ttgggcgggc
    61141 agcgagagtt gcgcgaggaa tgctttactg tcacccggca gcgaatcatc gtagttacgt
    61201 ggcacggcct tttgcaccag ctgcgaaagc gcctgatttt gataacgcac cacagtttcg
    61261 agcgggaatt tcatcggtgc tgctggcgtg gactcctctg gcaatggttg ttctggcgtc
    61321 atctgtttaa ccatcatctc tgcaagcccc agacctttgc ccgccgtcat ctgttgggca
    61381 atctgctggt catacatact ggtatacagg cgagtgtgct cgctgctgaa caggccatct
    61441 tttggtaaag cgtcgcgcat gcttttcaac atcatctgca cgaacatccc ttccacctga
    61501 cgggccaccg gacggatatt tgccgccgga tcttcgcccg ctttcgcctt tagttcgttg
    61561 agtgattgcg catcccaggc cgcacttgcc agtagtttgc tgtcgctgat catcagatga
    61621 tttccagttt tgcccgcaga catcccgcac tttgcattga ttgcagtatg gacatcagat
    61681 ccatcggcgt agcgcccagc gcattgagcg cgcgcaccac gttattgagg ctggcgctgg
    61741 aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc gatctgcgtt tgtggagtaa
    61801 ccacagtctg tccaccacca aacggtgtat ctggctggct gacattggcc tgacgattga
    61861 ctgttactga gagatttccc tgcgctaccg cgcagctgtc gagggtcact tcgcgattca
    61921 tcaccaccga accggtgcgc gagttaatca ctactttagc gtcctgcggg gtgacattaa
    61981 cctgcatatt ctggatatct gcaaggaaac ggacctggga actgttgcca cttggtacgc
    62041 gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc atatccacgc acgcggttga
    62101 tggtgtcagc gatttgctgc gccatgctga aatcttcgtc gttaagttgc aaattaaggg
    62161 tattcccgac gccaaactgg ctgggcaatt cacgttcaat aaccgcacca ttggtgatcc
    62221 gtccaccgtt cagttggttc acctgaacac tgctaccgcc agcggaggct cctgcgccgc
    62281 caaccagaat attgccctgc gccagcgcat acacctgact gtcaacgccc ttaagcggcg
    62341 tcatcaacaa cgtacctcca cgcaagcttt tggcatttcc catggaagaa accaccacat
    62401 cgatggtttg cccctgacgt ccaaacggag gaagtgactc tgtcaccatt accgcagcga
    62461 cgttttttag ctgcatattg gtgcccgtcg gaacggtaat tcccagctgt gagagcatgt
    62521 tattaagcgt ttgtgtggta aacggcgtct gggttgtctg gtcaccggtg ccatccagcc
    62581 ccaccaccag accatagcca atcagtgagt tttgccttac cccctgaaca ctggtgagat
    62641 cgcgaatacg ctcagcctga gccgccgtcg taaccagtag aagaattaat gcagagagaa
    62701 atttaatcac cacagcctca cttacattgg cgacaggtta aggaagaaac gctgcaacca
    62761 gcccatattt tgcgcttcgt taatgtagcc attgcctacg tattcaatgc gcgcatccgc
    62821 cacctgagta gacggtacgg tattgctgcc gctgatagtg cgtggattaa ccacgccaga
    62881 gaagcgaata aattcggtac cctgattaat ggcaatctgt ttttcaccca ccacatgcag
    62941 gttgccgttg accagtacct ggtcaaccgt caccgtcaac gtgccgctaa aggtattgct
    63001 ggcattggcc ccgccctttc cgttgaacgt gttaccaccg gaggcttcga catcggcacg
    63061 agcgttacca aacagcccct gcaaatagcg cggcacagta tcaaagccaa aattagtttt
    63121 accgtcacgg ctggcattcg cagaggagct tttgctggcg ctgacgttct cctgcaacac
    63181 gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct tcaaacagcg gttgatagcc
    63241 atagttaatc ggctgagcag actggaaaat agaaccgttg gcgacgggcg tcggaccggg
    63301 aaccggttgt gcactggtcg ccccctgcac cagcggcgtg gagggtatcc aggcgcagcc
    63361 ggttagtgaa agcaccaaca agctggaaat ggcataagta tgcgcagcgt ttttttgcat
    63421 ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc accggttaag ccttagagtt
    63481 gcgtcagttt ttgcagcatc tgatcggtgg tggacaccgc tttactgttg atttcgtaag
    63541 cgcgttgcac ctgaatcata ttgaccagtt cttccgccac gttgacgtta gacgtttcaa
    63601 catacccttg atacagcagt cccgcgccgt tcaggcccgg cgtgctttcg ttcggtacac
    63661 cagaggattg cgtttcggtg tagaggtttt cgccaatgct ctccagcccg gtgtcattca
    63721 taaaggtggt gagattgagc tgcccaacct gaaccggagc tgcctggcct tgttgggtta
    63781 cgctgaccac gccatcacga ccgatggtga tacttaacgc attcgccgga atggtgatcg
    63841 ctggctgcac ctgaaaacca ccagctgccc gttctgatcc acctggaaag agccgtcacg
    63901 ggtataggct gatgaaccat ctggcaacat cacctggaaa aagccctgcc ctttaatcgc
    63961 gacatctttg ctgttgttgg tctgcgacag gtttccctgg ctgtgtaagc gttcagtggc
    64021 gaccgggcgt acccccgtgc cgatttgtaa tccggagggt aaggtggttt gttcggaaga
    64081 ctgtgccccc ggctggcgaa tggtttgata aagcagatct tcaaacaccg cgcgctgacg
    64141 cttaaaaccg ttagtactga cgtttgccag gttgttggca atgacgtcca tattggtttg
    64201 ctgggcgtca aggcccgttt tggcgatcca taatgaactg atcataaaat gtcctgtagt
    64261 ggattagtga caaagtgcat atgtcttctt gccggatgcg gcgtaaacgc cttatccggc
    64321 ctacaagtgc tcgcaaaatc gataaattgc gattgccccc atagacccga taaacttgcg
    64381 catcgggcaa cttatacttg tcatgtatcc tttcaattaa ctcatcgaca gcagttggtt
    64441 ggcacggcct gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg
    64501 cgcgctggca atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac
    64561 ccccgacatc acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt
    64621 ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt
    64681 caccagtttc agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc
    64741 cgagattgtg ccatcggcag caatagtgat ttccgctcct tccggcacag caattggccc
    64801 cgcctcgcct atcacctgat gcccctgaat tgtcagttgc ccggtgggat caacctgaat
    64861 gctgccatta cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccagccatc
    64921 ctgctgcaac gcgacgtcca gcgggcgcga ggtgtaatcc attttgccgg gcgtcatatc
    64981 tgcgcccggc gttgacgccg tgaccaacgt gcgcgtgggc agagaaagcc cttccactgg
    65041 caccgcgcgt aaagcattca actgcgcgcg aaaaccgggc gttgaggcat tggccagatt
    65101 actggcggtt accgcctgtt gattcagtgt ctggctggct gctcccatcg cggtataaat
    65161 tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaaccagc gtgttgagga
    65221 tctggtcctg agttttgatg gtctgggcgt tagactgata gttacgctgg gcaacgatca
    65281 tattgaccag ttctttactg agatcgacat tggacgcttc caacgcaccg ttggtcaggg
    65341 tgccaaagtt gcccgtcccg gctgtcccca acagcgccac gccagaagat tgcgtcgcag
    65401 accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc gccagtacaa
    65461 tctgccccag cagttgtgtt tgttcgttgg aatagttgcc gacaaccgta ccgtcatcat
    65521 tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt gccacaatat
    65581 tgttagcgcc ggtattttgc tgcatggagt tgaggaagct caggctaaac gtggcgggat
    65641 ctgcgccgtt aattgcgcca gtggtaatat tctctgttgg atttgaggtc agaacgccat
    65701 tggcattaaa caccagcttc attgcaggct cggctgtacc tgttggatca ctgctatcct
    65761 gggtgtagac ctgccagtta ttatccccgg tcttcacaaa gtagacgctc atgtcatgag
    65821 cattaccctg actgtcgaaa acagtcaccg aacctttttt gttatagcta tccgcattgc
    65881 tggcgctgaa tggcgtaaca gtaggaagcg gatcactgga attcaggttg atctgcatcg
    65941 acgccgtggt ggtagttttc gctgccatca gggtattcgg gatcgaaata ttggtcggat
    66001 tcgccccttg ctgaatagtc ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac
    66061 cttgcatatt caccaggtta cggttttcat ccagcttaaa ttgtccgtta cggctgtaga
    66121 acaccgaacc gttgctgtct accagacgga aaaaaccgtt ctggctgata gcaacgtcca
    66181 gacctcgccc ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt
    66241 ttacccccag tcccactttc gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt
    66301 taaagccgta ggtggcggag ttggcgatat tgttgccaat aacatcgagg ttggtggcgg
    66361 cagcgtttaa tccgctaacc gcttgagaaa aggccatgac tgactcctga aatgtgaagg
    66421 cttaaattat ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg
    66481 tattaccgct gttgccgcgg agcacaccct gcaccagagc aaactgcagc ggctgggcaa
    66541 ccagttgtgt accaccgtta ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag
    66601 tgctgccatc agtcaacgta ccgtcccagg tgaaactgtg gactccggcg gtcagttcac
    66661 caatatcaat ggtgcgcaca accgcgccat ttttatcggt gatggtggcc gtaactttgt
    66721 ctgccgcctg ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac
    66781 tgccggttcc cgcaagaaca gtggtgccgg ggatcatcac gccgtgaccg atcaggttac
    66841 tggcctgtaa cgactggctg ttatcaatct gtccggaaat agatccgagc gtggtattga
    66901 gtttttcaat cccgctgacc gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt
    66961 ccattggatt ggtcgggtcc tggtttttca gctgcgccac caacaaagtc agaaaactgc
    67021 tttgtaagtc tgcggcgttg ctgcccgtga gcgaactact gctggtggta ctgacgccgg
    67081 tatttgtcgg atcggtggtg gttaccgcaa tggacatagc tttctccttt attgaccgag
    67141 cgtaagggtt ttcagcatca tgcttttcac cgtgttgagc acttcaacat tggcctgata
    67201 gctgcgtgac gccgacatgg tgttaaccat ctccccgacg acatcaacgt tcggcatttt
    67261 tacgtagccc tttgcatccg ccagcggatt acccggttca taaaccagtt tgtccggggc
    67321 ctgactttct ataacatcgg caacctttac gccgcctgtc gcagcacctg gtgcagcgtt
    67381 aacctggaat accacctgtt ttgcccgata tggctgtcca tcgggaccgg tcacgctatc
    67441 agcattcgcc agattactgg ccgccacgtt caagcgctgg gactgggcag ttaacgccga
    67501 cccggcgata tcaaaaatat tcagcagtgc catccgttaa tttccgctct gtaaaacgtt
    67561 catcatgcct ttgatttgcc cgctcaacgc gctaaggctc atctggtatt gcaggctgtt
    67621 atcggcaaac tgggtgcgtt cgcgatccat atcgacggta ttaccgtcaa gcgaaggctg
    67681 gtccggaata cggtattgca gttctgcggt aggaggcgtc agcgcctgcg ccggaatgtg
    67741 ttgcgttgag gtcatcgtca gtgcaaccac actggttgca tcccgtccac gttgcatgac
    67801 ttttttaagt tcactggcaa aatcgatatc gcgcgcctga taaccagggg tatcggcatt
    67861 ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc agattgagcg cctcttgttg
    67921 aaaacgtaag gcggcgtcga gcttatcgag catatctcct ccgcaggtat caaaattctg
    67981 ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc
    68041 tatttatgcc gttgatgacc attgcggaca ggtacaattc acgttgtaga aatggctggg
    68101 ggcgaaaatg ctggcaataa aacgtagcgt ggcgatcatc tcgatactat tcactccgtt
    68161 aagtgcggcg agtaatctca catcgcaatt gcaaaccttt tttagcgccc agctcgcggg
    68221 ggtaagtgat gaggttcgtg tttctattcg tacagcgccc aatctactac cgccgtgcga
    68281 gcagccattg ctttcgatga gcaataattc ccgcctgtgg ggcaatgtga atgtgttggc
    68341 acgctgcggt aacgacaaac gatatttaca ggttaatgta caggccacag gaaattatgt
    68401 ggttgccgcg atgcccattg tgcggggagg aaagctggaa gctggcaatg tcaaactaaa
    68461 acgcggacgg caggataccc tgccaccgcg tacggtgctg gatatcaatc aacttgttga
    68521 tgccgttagc ctgcgcgatc tatcacccga tcaacctatc cagttaaccc agtttcgcca
    68581 ggcatggcgg gtaaaagcgg gacaacgcgt caacgtgatc gccagcggtg acgggtttag
    68641 cgccaacgca gaaggtcagg cgctgaacaa tgcagccgtc acacagaatg cgcgggtgcg
    68701 catggtatcg ggactggtgg tcagcggcgt tgttgatgca gatgggaata ttcttataaa
    68761 ccagtaagct gttaaagatt acccgtccct tgccgataaa taagcaacac atgataaaag
    68821 cgccctcaat gaggaataaa ccatgagtat tgatcgcact tcgcctctga agcccgtaag
    68881 caccgttcaa ccacgcgaaa ccactgacgc gtcggtaacg aacacccggg cggcaaaaac
    68941 aacagcctcc accagcacca gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc
    69001 cggcagcagt gatatcaatc ttgaacgtgt cgaagcgtta aaactggcga ttcgtaacgg
    69061 tgaactaaaa atggacaccg gcaaaattgc cgatgcgctg ctcaacgaag cgcagcaaga
    69121 cttgcagagt aactgatcgt atgacacgtc ttgcagagat cctcgaccag atgtccgctg
    69181 tgcttaacga tctcaaaacg gtaatggatc aagaacagca acatctctct atggggcaga
    69241 tcaacggcag ccagttgcaa tggattacag aacaaaaaag ctcactgctg gcgacgctgg
    69301 attacctcga acagttacgc aggaaagaac ccaatacagc aaatagcgtt gatattagtc
    69361 aacgctggca ggaaattact gtgaaaacgc agcaactacg ccaaatgaat caacataacg
    69421 gctggttact ggaaggacag attgagcgca atcaacaggc gctggaaatg ttgaaaccgc
    69481 atcaggaacc gacgctatat ggggcgaacg gtcagacctc aacaacccat cgcggcggta
    69541 aaaagatttc gatctgaata ttatctccgg cctgcactgc aggccggaat gcattgttac
    69601 accgtccggc gggcaaattc tttaactttg aagcccagta ccgccagtgc agcgaagtac
    69661 gcggcaatcc ccgccagcac gaccgccatt aaacgcagta agcgccaggg catggtaccc
    69721 aatgaccact ccggcatgat atgtaacata cctaaaagca cgccagacat caccagtacc
    69781 gccaccacca gacgcaacag aaacgccatc cagccgggtt gcggagtaaa gattttctgc
    69841 ttacgcaact gccagtaaag cagcgaagca ttcagacacg ccgccagacc aatagaaagt
    69901 gacagcccag catgtttcaa cggaccaata aacgccaggt tcatcaattg cgttaaaatc
    69961 agcgtaacga tggcaatttt cactggcgtt ttaatgtcct ggcgggaata aaagccagga
    70021 gccaacactt tcactacaat caggccaatc aaacccaccg agtaggcaat taacgcccgc
    70081 tgggtcatca gcgcatcaaa cgcggtaaat ttaccgtact ggaacagcga aacggttaac
    70141 ggacccgaaa gaatgcccaa cgcaaccgca ctcggcagcg ccaacaggaa acaaagacgc
    70201 aacccccagt ccatcaaacg gttgtattca tcatgattgc cactggcaaa acttttcgac
    70261 agcgacggca gcaaaatggt gccaagcgcc acgcccagca caccggaggg aaactccatt
    70321 aagcggtcgg cgtaatacat ccaagacacc gaaccggaag caagaaacga ggcaaaaatg
    70381 gtgttgatga ttaaggagat ctggctcaca gagacgccaa ggatcgccgg tcccatctgt
    70441 ttcaccacgc gcattgctcc ggcatcgtgg aagttaatgc gcggcaggac cagcatgccg
    70501 atcttcttca ggtgcggtag ctgatacacc agctgcagga tgccgccgac cgtaacagcc
    70561 cacgccagcg ccagcaccgg tgggttaaag tacggtgcgg caaacagcgc gaaaccaatc
    70621 atgctgatgt taagcagtgt tggcgcaaac gccggaatcg agaagcggtt ccacgtattc
    70681 agaatcgctc ccaccagcga cgccagggag atcagcaaga tatagggaaa ggtaatcttt
    70741 agtagctggc tggtcagggc aaatttgtca gctgtgtcag cgaagcctgg cgcggtcacc
    70801 atgatcaccc acggtgcggc gagcatgcca gcgaccgtca caaccgccag cgcaagtgtc
    70861 agcaggccag aaacataaga gacaaagacc cgcgtggcgt cttcaccctg cttactttta
    70921 tattccgcca gaatcggtac aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt
    70981 aacaagttag gaagtttaaa agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag
    71041 attctggcga caattgcgtc tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag
    71101 ctgacggcgg ccagcgattt taataaattc atcggtgttc taatccagac ccacgggcaa
    71161 aacgcctgca aggcaggcgc tgaaagaggc gaatagtcta cctggattat ggtgaattgc
    71221 taccgccaga tgttacaggg tttattcact catcgcatcg cgccagatct tgtcaacgat
    71281 acgttgcgcc agcactgcct gttcgccggc ggtttgcaga actgtctgat tttgcacaca
    71341 ttcaatgaag tgccgcgcac agccgacaaa tccacgttgc tcaagagtgc tctgccaacc
    71401 aggaatcggt ttatgcacta cgccctgccc gcgctcctca cgccattcgc gcatatccgt
    71461 aatgtcgatg agcgcatcgt cagtcacggc ctgcacggtt tcacgctgac ttccggcacg
    71521 gcgatgcata caggtggtga tctgcaaagg atcggccgaa aaatggtgct cggcaaacag
    71581 catttcgcca gcgtcgttag tcagtagcgt accgccatcc agagaggctt tgccgcccga
    71641 caaccacagc gcggtatcca ccacatgcag ataatcatcc agcaacgtga aataaagatc
    71701 gtgtggcccg acactattgc tacgatgttt atccattctt agcgaagctg cggtagcgag
    71761 ttgcgttttt aactcaccgt agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt
    71821 cagttttttc cgcgccgcca gttccaccag ccgttcagca tcgcgcagat tttctgccag
    71881 cggtttatcg acacagacat gtacacccgc attgagtaac gtactgacca cgtcaaagtg
    71941 gctggcggtg ctggaatgca caaaaaccgc atcgcaactg gcggcaaggc tggataacga
    72001 atcggcataa ggaatgcgcc agctttcaca aattggcagg gctttcgcgc gcgtaggcga
    72061 ccaggctcct tgtaacatcc agtcagacgc tgccgccagc accggtaacc acgctttttg
    72121 cgcaatgccg cctaatccca caacgccgat acgtaatttt ttcactttaa tctcccagat
    72181 gggccagcaa cgaatcaaga cgctgtttca gttctgccac ttcgatttcc agggcttcga
    72241 cgcgggcctg taaatcacca tcaaccgcgt tcgacatatc cgttaccgcc ggctgatcct
    72301 ctacctcacc actgaaaaga tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca
    72361 gacgcaccac aaaaggacca tcttcgcgat ttgccagttg ttccagcgtc gactccactt
    72421 ccgccatatc gctgaattca tacattcgcg cggcgcggct gcgcagttca cccggcgtct
    72481 gggcaccacg caataacaac gtggtgatta acgccacttc cgctgcgctc agtttcagat
    72541 cgccaaattc tgaattacaa aaacgttgtt catatttggt gacccgatta ccaaagccac
    72601 tcaccgtgcg cagataatgg cgtttgacca gattatccag ttgctcctgc acttcagatt
    72661 cactcagatt catgaccggt tcacggttcg ttttctgatt acaggccgtg actacaccat
    72721 tgactgagag cgggtattgc tccggcgtgg tcacctgctt ttccagcaag caaccaatca
    72781 cgcgagcttc aagggcagta agttgatatt tcatgagatt ctccttagcg gccgggcgtc
    72841 cagtctgggg tagttaatgc cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga
    72901 tagtctttcg catagccttc tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt
    72961 ttattgtgcg gcatataatt agccataatg cgatgaatat gttgggtgcg ctgcatataa
    73021 cgaatggctg cggtcagggc ttcaaacatg agtcctttgc cctgccattt ttgcccaatc
    73081 gaataaccga gatagcaggc atgaaaagag ccacgaacaa cattggaaaa attggcaacg
    73141 ccaataatct ctttttcgtc cgggtcgaat aagccaaagt agaaagctga accttgttta
    73201 tgaaattcgt taatcatccc cagcctggcc tgccagcctg atggataaca gtggctttcg
    73261 tcgcgcactg gctcccaggg cttgaggaaa tggcgattct ctgcgtaata atccgcaaga
    73321 cgccaggcat cacgatcatg caccagacgc acgaccagtc ggtctgtggt taagcgcact
    73381 tttggcacgt tactgcgata gccaaacatt ctatacctac tccttcccgt aacgtcttta
    73441 atacgctgta agtattacta tacctgcgca accagaccct gtgaaaagtc caaaatgcca
    73501 ttttctgcat tattcacata tttgataaaa actctctatc aaagcgatgt tttgataaaa
    73561 aaatattgtc cccgaagtga tgggaaaagt cttagcggca atgcagaata gaaagtgctc
    73621 atcttttatt tcccgggagg ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa
    73681 tatttcctgc tcatcgataa tatgctggtc gtgctggggt tctttgttgt cttcccgctg
    73741 atctctatcc gcttcgttga tcaaatgggc tgggccgccg tcatggtcgg tattgctctc
    73801 ggtctacgcc agtttattca gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc
    73861 tttggtgcca aaccgatgat tgttaccggt atgctgatgc gcgccgccgg attcgccaca
    73921 atgggtatcg cccacgaacc gtggctattg tggttttcat gcctgctctc gggactcggc
    73981 ggcacgttgt ttgatccgcc gcgttcggcg ctggtggtga aactaatccg tccacaacaa
    74041 cgttgtcgtt ttttctcgct gttgatgatg caggacagtg ccggtgcggt cattggcgca
    74101 ttgttgggga gctggctgtt gcaatacgac tttcgcctgg tctgcgccac aggggccgtt
    74161 ttgtttgtgc tgtgtgcggc gttcaatgcg tggttgttac cggcatggaa actctccacc
    74221 gtacgcacgc ccgttcgcga aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc
    74281 tatgtcctga cgctggcggg ttactacatg ctggctgtac aagtgatgct gatgctgcca
    74341 attatggtca acgacgtggc tggcgcgccc tctgccgtta aatggatgta tgccattgaa
    74401 gcgtgtctgt cgttaacgtt gctctaccct atcgcccgct ggagtgaaaa gcattttcgt
    74461 ctggaacacc ggttgatggc tgggctgttg ataatgtcat taagcatgat gccggtgggc
    74521 atggtcagcg gtctgcaaca acttttcacc ctgatttgtc tgttttatat cgggtcgatc
    74581 attgccgagc ctgcgcgtga aaccttaagt gctttgctgg cggacgcaag agctcgcggc
    74641 agctatatgg ggtttagccg tctgggtctg gcgattggcg gcgctattgg ttatatcggt
    74701 ggtggctggc tgtttgacct gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg
    74761 ctgggaatta ttggcatctt cactttcctt gcgctgggtt ggcagtttag tcagaaacgc
    74821 gcaacgcgtc gtttgctgga acgcgacgcc tgatttgata ctcccccctg actcctccat
    74881 actgaaagtg aactgacagc caaatggagg agtcacgtga agctatacat ttacgatcac
    74941 tgcccttact gcctcaaagc ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta
    75001 catgttctgc tcaacgacga cgcagaaaca cccacccgga tggtcggtca aaaacaggtt
    75061 cccattctgc aaaaagatga cagccgctat atgccagaaa gcatggatat cgttcactat
    75121 gtcgataaac tcgacggcaa accgttactg accggcaaac gttcccctgc cattgaagag
    75181 tggctgcgca aggtcaatgg ctacgccaac aaactgctgt tgccgcgttt tgccaaatcg
    75241 gcatttgatg agttttctac tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc
    75301 agcgcgggta attttgccga cctgctggcc cactctgacg gtctgattaa gaatatcagc
    75361 gatgatttgc gtgcgctgga caaactgatc gtcaaaccga acgccgtgaa tggcgaactt
    75421 tcggaagatg atattcagct atttccgcta ctgcgtaatc tgacgctggt agccggaatc
    75481 aactggccaa gccgcgttgc tgattaccgc gataatatgg cgaaacagac acaaatcaat
    75541 ttgttatcat caatggcgat ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg
    75601 ccgccgtttt cagttttttc tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg
    75661 tcaggatatt gacgcagatt tcatgtcgag gactaccatg aacaagtttt tatttgccgc
    75721 tgcattgatc gtcagtggcc tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac
    75781 cgaacaagaa attaaccagt cgcttgcgaa acataataat ttctcaaaag atatcggttt
    75841 acccggcgtg gctgacgccc atattgttct gacaaacttg atcagccaaa ttggtcgcga
    75901 agagccgaat aaggttaccc taaccggaga cgccaatctg gacatgaact ccctgttcgg
    75961 tagtcagaaa gcgaccatga aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa
    76021 aggtgcgatc ttcctgaaag agatggaagt ggtcgatgcg acggtacaac cggaaaaaat
    76081 gcaaacggtg atgcaaacgt tgcttcccta tttgaaccag gcattacgca attactttaa
    76141 ccagcaacct gcttacgtcc tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa
    76201 actggcgaaa ggcattgaag tgaagccagg cgaaattgtc attccgttta cggattaatg
    76261 acgagggcgc attcgcgccc tttatttttc gtgcaaagga aaacgtttcc gcttatcctt
    76321 tgtgtccggc aaaaacattc cttcagccgg agcatagaga ttaatgactg caccatccca
    76381 ggtattaaag atccgccgcc cagacgactg gcaccttcac ctccgcgatg gcgacatgtt
    76441 aaaaactgtc gtgccgtata ccagcgaaat ttatggacgg gctatcgtaa tgcccaatct
    76501 ggctccgccc gtgaccacag ttgaggctgc cgtggcgtat cgccaacgta ttcttgacgc
    76561 cgtacctgcc gggcacgatt tcaccccatt gatgacctgt tatttaacag attcgctgga
    76621 tcctaatgag ctggagcgcg gatttaatga aggcgtgttc accgctgcaa aactttaccc
    76681 ggcaaacgca accactaact ccagccacgg cgtgacgtca attgacgcaa tcatgccggt
    76741 acttgagcgc atggaaaaaa tcggtatgcc gctactggtg catggtgaag tgacacatgc
    76801 agatatcgac atttttgatc gtgaagcgcg ctttatagaa agcgtgatgg aacctctgcg
    76861 ccagcgcctg actgcgctga aagtcgtttt tgagcacatc accaccaaag atgctgccga
    76921 ctatgtccgt gacggaaatg aacggctggc tgccaccatc actccgcagc atctgatgtt
    76981 taaccgcaac catatgctgg ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct
    77041 caaacgcaat attcaccaac aggcattgcg tgaactggtc gccagcggtt ttaatcgagt
    77101 attcctcggt acggattctg cgccacatgc acgtcatcgc aaagagagca gttgcggctg
    77161 cgcgggctgc ttcaacgccc caaccgcgct gggcagttac gctaccgtct ttgaagagat
    77221 gaatgctctg cagcactttg aagcattctg ttctgtaaac ggcccgcagt tctatgggtt
    77281 gccggtcaac gacacattca tcgaactggt acgtgaagag caacaggttg ctgaaagcat
    77341 cgcactgact gatgacaccc tggtgccatt cctcgccggg gaaacggtac gctggtccgt
    77401 taaacaataa aaatcgtagc ttcctgttgt cattaggtta tttaacctgt ataaataacc
    77461 agtatattca acagggggct attatgcgaa ttgaagtcac catagcgaaa acttctccat
    77521 tgccagctgg ggctattgac gccctggctg gcgaactttc ccgccgtatt cagtatgcgt
    77581 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
    77641 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
    77701 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
    77761 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
    77821 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
    77881 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
    77941 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
    78001 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
    78061 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
    78121 accccaaata agtccgagca ggaagggact gaagttggtc aaacgctctg gttaaccact
    78181 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
    78241 gatttccagg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
    78301 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
    78361 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
    78421 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
    78481 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
    78541 catgcttatg gtgaaggcga aaagtatgtc ccgctggtcc tccgcgcgca aacgctgtgg
    78601 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
    78661 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
    78721 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
    78781 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
    78841 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
    78901 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
    78961 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
    79021 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
    79081 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
    79141 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
    79201 gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg gttcggaagc cttcccgttc
    79261 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
    79321 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
    79381 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
    79441 gattttgcgc aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
    79501 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
    79561 ggctgttgca ctatctca
//
LOCUS       DAJVYM010000003        74293 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVYM010000003 DAJVYM010000000
VERSION     DAJVYM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.003"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..941
                     /locus_tag="OQS60_000169"
     CDS             <1..941
                     /locus_tag="OQS60_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS2386041.1"
                     /translation="MLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            977..1663
                     /gene="mlrA"
                     /locus_tag="OQS60_000170"
     CDS             977..1663
                     /gene="mlrA"
                     /locus_tag="OQS60_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS2386042.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1723..1830
                     /locus_tag="OQS60_000171"
     CDS             1723..1830
                     /locus_tag="OQS60_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS2386043.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="OQS60_000172"
     CDS             complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="OQS60_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS2386044.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="OQS60_000173"
     CDS             complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="OQS60_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS2386045.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="OQS60_000174"
     CDS             complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="OQS60_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS2386046.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="OQS60_000175"
     CDS             complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="OQS60_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS2386047.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5799..8096)
                     /gene="bglX"
                     /locus_tag="OQS60_000176"
     CDS             complement(5799..8096)
                     /gene="bglX"
                     /locus_tag="OQS60_000176"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS2386048.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8292..10007
                     /gene="dld"
                     /locus_tag="OQS60_000177"
     CDS             8292..10007
                     /gene="dld"
                     /locus_tag="OQS60_000177"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS2386049.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10046..10978)
                     /gene="pbpG"
                     /locus_tag="OQS60_000178"
     CDS             complement(10046..10978)
                     /gene="pbpG"
                     /locus_tag="OQS60_000178"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS2386050.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11152..11739)
                     /locus_tag="OQS60_000179"
     CDS             complement(11152..11739)
                     /locus_tag="OQS60_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS2386051.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11909..12487
                     /locus_tag="OQS60_000180"
     CDS             11909..12487
                     /locus_tag="OQS60_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2386052.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12617..13378)
                     /locus_tag="OQS60_000181"
     CDS             complement(12617..13378)
                     /locus_tag="OQS60_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2386053.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13431..14867)
                     /gene="mdtQ"
                     /locus_tag="OQS60_000182"
     CDS             complement(13431..14867)
                     /gene="mdtQ"
                     /locus_tag="OQS60_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS2386054.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15091..15174
                     /gene="yohP"
                     /locus_tag="OQS60_000183"
     CDS             15091..15174
                     /gene="yohP"
                     /locus_tag="OQS60_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS2386055.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15547..16494)
                     /gene="dusC"
                     /locus_tag="OQS60_000184"
     CDS             complement(15547..16494)
                     /gene="dusC"
                     /locus_tag="OQS60_000184"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS2386056.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16733..17131
                     /locus_tag="OQS60_000185"
     CDS             16733..17131
                     /locus_tag="OQS60_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS2386057.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17128..17823
                     /locus_tag="OQS60_000186"
     CDS             17128..17823
                     /locus_tag="OQS60_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS2386058.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17953..18837
                     /gene="cdd"
                     /locus_tag="OQS60_000187"
     CDS             17953..18837
                     /gene="cdd"
                     /locus_tag="OQS60_000187"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS2386059.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18987..19706
                     /gene="sanA"
                     /locus_tag="OQS60_000188"
     CDS             18987..19706
                     /gene="sanA"
                     /locus_tag="OQS60_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS2386060.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19709..19948
                     /locus_tag="OQS60_000189"
     CDS             19709..19948
                     /locus_tag="OQS60_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS2386061.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20142..21380
                     /gene="preT"
                     /locus_tag="OQS60_000190"
                     /pseudo
     CDS             20142..21380
                     /gene="preT"
                     /locus_tag="OQS60_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21374..22609
                     /gene="preA"
                     /locus_tag="OQS60_000191"
     CDS             21374..22609
                     /gene="preA"
                     /locus_tag="OQS60_000191"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS2386062.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22736..23746)
                     /gene="mglC"
                     /locus_tag="OQS60_000192"
     CDS             complement(22736..23746)
                     /gene="mglC"
                     /locus_tag="OQS60_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS2386063.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23762..25282)
                     /gene="mglA"
                     /locus_tag="OQS60_000193"
                     /pseudo
     CDS             complement(23762..25282)
                     /gene="mglA"
                     /locus_tag="OQS60_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
     gene            complement(25343..26341)
                     /gene="mglB"
                     /locus_tag="OQS60_000194"
     CDS             complement(25343..26341)
                     /gene="mglB"
                     /locus_tag="OQS60_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS2386064.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26621..27661)
                     /gene="galS"
                     /locus_tag="OQS60_000195"
                     /pseudo
     CDS             complement(26621..27661)
                     /gene="galS"
                     /locus_tag="OQS60_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27803..28960)
                     /locus_tag="OQS60_000196"
     CDS             complement(27803..28960)
                     /locus_tag="OQS60_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS2386065.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28977..29645)
                     /gene="folE"
                     /locus_tag="OQS60_000197"
     CDS             complement(28977..29645)
                     /gene="folE"
                     /locus_tag="OQS60_000197"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS2386066.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29903..30739
                     /gene="yeiG"
                     /locus_tag="OQS60_000198"
     CDS             29903..30739
                     /gene="yeiG"
                     /locus_tag="OQS60_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS2386067.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30771..32762)
                     /gene="cirA"
                     /locus_tag="OQS60_000199"
     CDS             complement(30771..32762)
                     /gene="cirA"
                     /locus_tag="OQS60_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS2386068.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33054..34523)
                     /gene="lysP"
                     /locus_tag="OQS60_000200"
     CDS             complement(33054..34523)
                     /gene="lysP"
                     /locus_tag="OQS60_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS2386069.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34728..35609)
                     /locus_tag="OQS60_000201"
     CDS             complement(34728..35609)
                     /locus_tag="OQS60_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2386070.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35708..36757
                     /locus_tag="OQS60_000202"
     CDS             35708..36757
                     /locus_tag="OQS60_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS2386071.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36831..37688
                     /gene="nfo"
                     /locus_tag="OQS60_000203"
     CDS             36831..37688
                     /gene="nfo"
                     /locus_tag="OQS60_000203"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS2386072.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37692..38780
                     /locus_tag="OQS60_000204"
     CDS             37692..38780
                     /locus_tag="OQS60_000204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS2386073.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38836..40086)
                     /locus_tag="OQS60_000205"
     CDS             complement(38836..40086)
                     /locus_tag="OQS60_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS2386074.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40186..41127)
                     /gene="rihB"
                     /locus_tag="OQS60_000206"
     CDS             complement(40186..41127)
                     /gene="rihB"
                     /locus_tag="OQS60_000206"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS2386075.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41257..41955
                     /gene="yeiL"
                     /locus_tag="OQS60_000207"
     CDS             41257..41955
                     /gene="yeiL"
                     /locus_tag="OQS60_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCS2386076.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42022..43272)
                     /locus_tag="OQS60_000208"
     CDS             complement(42022..43272)
                     /locus_tag="OQS60_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS2386077.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43366..44304)
                     /gene="psuG"
                     /locus_tag="OQS60_000209"
     CDS             complement(43366..44304)
                     /gene="psuG"
                     /locus_tag="OQS60_000209"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS2386078.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44292..45233)
                     /locus_tag="OQS60_000210"
     CDS             complement(44292..45233)
                     /locus_tag="OQS60_000210"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS2386079.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45691..47382)
                     /gene="fruA"
                     /locus_tag="OQS60_000211"
     CDS             complement(45691..47382)
                     /gene="fruA"
                     /locus_tag="OQS60_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS2386080.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47399..48337)
                     /gene="fruK"
                     /locus_tag="OQS60_000212"
     CDS             complement(47399..48337)
                     /gene="fruK"
                     /locus_tag="OQS60_000212"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS2386081.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48337..49467)
                     /gene="fruB"
                     /locus_tag="OQS60_000213"
     CDS             complement(48337..49467)
                     /gene="fruB"
                     /locus_tag="OQS60_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS2386082.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49835..51016
                     /gene="setB"
                     /locus_tag="OQS60_000214"
     CDS             49835..51016
                     /gene="setB"
                     /locus_tag="OQS60_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS2386083.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51013..51267)
                     /locus_tag="OQS60_000215"
     CDS             complement(51013..51267)
                     /locus_tag="OQS60_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS2386084.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51422..51994
                     /gene="yeiP"
                     /locus_tag="OQS60_000216"
     CDS             51422..51994
                     /gene="yeiP"
                     /locus_tag="OQS60_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS2386085.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52217..53683
                     /locus_tag="OQS60_000217"
     CDS             52217..53683
                     /locus_tag="OQS60_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS2386086.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53801..54787
                     /gene="yeiR"
                     /locus_tag="OQS60_000218"
     CDS             53801..54787
                     /gene="yeiR"
                     /locus_tag="OQS60_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS2386087.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54826..55539
                     /gene="lpxT"
                     /locus_tag="OQS60_000219"
     CDS             54826..55539
                     /gene="lpxT"
                     /locus_tag="OQS60_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS2386088.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55951..56517
                     /gene="mepS"
                     /locus_tag="OQS60_000220"
     CDS             55951..56517
                     /gene="mepS"
                     /locus_tag="OQS60_000220"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS2386089.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56698..58254
                     /locus_tag="OQS60_000221"
     CDS             56698..58254
                     /locus_tag="OQS60_000221"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS2386090.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58336..60150
                     /locus_tag="OQS60_000222"
     CDS             58336..60150
                     /locus_tag="OQS60_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2386091.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60151..61245
                     /locus_tag="OQS60_000223"
     CDS             60151..61245
                     /locus_tag="OQS60_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS2386092.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61245..62270
                     /locus_tag="OQS60_000224"
     CDS             61245..62270
                     /locus_tag="OQS60_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2386093.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62272..63861
                     /gene="yejF"
                     /locus_tag="OQS60_000225"
     CDS             62272..63861
                     /gene="yejF"
                     /locus_tag="OQS60_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS2386094.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63865..64209)
                     /locus_tag="OQS60_000226"
     CDS             complement(63865..64209)
                     /locus_tag="OQS60_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386095.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64542..65662)
                     /locus_tag="OQS60_000227"
                     /pseudo
     CDS             complement(64542..65662)
                     /locus_tag="OQS60_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65690..66385)
                     /gene="rsuA"
                     /locus_tag="OQS60_000228"
     CDS             complement(65690..66385)
                     /gene="rsuA"
                     /locus_tag="OQS60_000228"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS2386096.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66535..68295
                     /locus_tag="OQS60_000229"
     CDS             66535..68295
                     /locus_tag="OQS60_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS2386097.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68420..68704
                     /gene="rplY"
                     /locus_tag="OQS60_000230"
     CDS             68420..68704
                     /gene="rplY"
                     /locus_tag="OQS60_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS2386098.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68843..69850)
                     /gene="yejK"
                     /locus_tag="OQS60_000231"
     CDS             complement(68843..69850)
                     /gene="yejK"
                     /locus_tag="OQS60_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS2386099.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70032..70259
                     /locus_tag="OQS60_000232"
     CDS             70032..70259
                     /locus_tag="OQS60_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS2386100.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70279..72039
                     /gene="pbgA"
                     /locus_tag="OQS60_000233"
     CDS             70279..72039
                     /gene="pbgA"
                     /locus_tag="OQS60_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS2386101.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72114..72190
                     /locus_tag="OQS60_000234"
     tRNA            72114..72190
                     /locus_tag="OQS60_000234"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72148..72150,aa:Pro,seq:ggg)
     gene            complement(72293..74293)
                     /gene="yejO"
                     /locus_tag="OQS60_000235"
                     /pseudo
     CDS             complement(72293..74293)
                     /gene="yejO"
                     /locus_tag="OQS60_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18238 a        19576 c        18244 g        18235 t
ORIGIN      
        1 caatgttagg tgaaaaaaga ctggtttcat ctgtaacaaa agaggatttg cttttcgtaa
       61 ggagagatct gttgaccggt tatcaaaagc tttctaacgg aaaaatttct tccataaaag
      121 gacgctcagt ggtcacagta aactactata tgacaaccat agctggaatg tttcaatttg
      181 caacagataa tggttataca tcaggaaatc catttaacgg tctgacacca ttaaaaaagt
      241 ccaagataga accagatccc ctcacccgtg acgaatttat tcgttttatt gaggcttgcc
      301 gtcatcaaca aacaaaaaac ctgtggatta tcgctgtata cacgggtatt cgtcacgggg
      361 agctggtatc actggcctgg gaagatatag accttaaagc gaggactata accatccgcc
      421 ggaattatac aaaactaggc gaattcactc caccaaaaac tgatgctggt accggaagaa
      481 cgattcatct agttcaacca gctattgatg ctcttaaaag ccaagcggaa atgaccatgc
      541 ttggaaaaca gcattctgta gaggtaaagc agagggaata tgggagaagt actgtgcata
      601 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
      661 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
      721 acagaaaatc gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gctggagcta
      781 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
      841 acggagcatg gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat
      901 ctgaaagtgt cccatgtatg ccccataaga aagtggggta aaataaaaac ttgtaaaatc
      961 agttagttta cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acgggttgct
     1021 gaaaccgcaa cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccggat
     1081 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
     1141 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
     1201 ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga
     1261 ttaccccgcc cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg
     1321 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
     1381 cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
     1441 gaatattcag gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg
     1501 gcgcattgat gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg
     1561 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag
     1621 ttggcaagaa caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg
     1681 cgctttactg tacaatcctt tcgttaacat aaggagtgca atatgcgcat agctaaaatt
     1741 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
     1801 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
     1861 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
     1921 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
     1981 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
     2041 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg
     2101 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
     2161 gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
     2221 gaacggcaat cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt
     2281 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc
     2341 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
     2401 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
     2461 gcggctggct gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca
     2521 gcggatcgcg caacatcttc atacgcttac gcctcctcca gcagatcctg aaaatgcagc
     2581 gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca
     2641 aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt
     2701 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttcg
     2761 ctgcgcccaa aaaactggcg gacaaaatcg tttgccggac gagtcagcat cgtcagcgga
     2821 ttgccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
     2881 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
     2941 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
     3001 atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga
     3061 ccaccggaaa gctgatgcgg ataacgctca cgtaaatttg actccagccc cagtagcgcc
     3121 attaattcgt cgatacgatc gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg
     3181 gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag
     3241 cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
     3301 atcactccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
     3361 gagccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
     3421 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc caaattcaat cattacgttt
     3481 caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat
     3541 taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac
     3601 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg
     3661 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg
     3721 caggaatcgc tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg
     3781 gatctggttc aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
     3841 cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcgtcccca gccacggaaa
     3901 ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac
     3961 ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca
     4021 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
     4081 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
     4141 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
     4201 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cactacaggc
     4261 cagcaatgcc agcgccgcgg ccagccagaa accactgccg aggctggtac gcgccagcgc
     4321 actgccattt tgcgccagtt gggtcgccgc ctttcctgct ccccacacca gcaatacgaa
     4381 gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc
     4441 tgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca
     4501 gagatgacgc ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc
     4561 cgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt
     4621 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
     4681 atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
     4741 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tctttcagca ccgactcacg
     4801 caccactggt gcaggcgcgt agataggttg cacgcctttc ggatcgctta aggtttgcag
     4861 ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt
     4921 ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg ccagccagtg acagtaactg
     4981 atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
     5041 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tctttcagat aacgactcag
     5101 gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatggtcca
     5161 ggtgttattc gctggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt
     5221 tttgactttc tcgtaacctt gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa
     5281 gaagaaagcg ccattaccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
     5341 cccgcgcacc acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct
     5401 ttccagcacc tgcaagatga tgttgccgag tagcgcgcct tcggtatcga tttttgaacc
     5461 gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga
     5521 acctgcccag acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca
     5581 gcgaattaag agaaaagcat agttgataat gacagagtta gcctgagcgg aaggattcag
     5641 ttgcagaatc agataaatac ttaaagggca tattttgccc ggcgtttctt gccggatgcg
     5701 gcgtaaacgc cttatccggc ctacaaaggt cgaaaattca gcaagttaag atatgaacat
     5761 caggcaaagg gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct
     5821 ttcttaacgc gtgcggaatc agtgccgata aagacattga acttgccagg ttcggcgtca
     5881 tatttcatct gttgattcca gaacttcagc gcctcgatat caatcgggaa gctgacagtc
     5941 tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgctt cactggacgg
     6001 ctcatggaag ccgtcacatc ctgcaagtac atctgtacta ccgtggcccc ttcgcgttta
     6061 ccggtgttcg tcacctgcac gctggcggtc actttgccgt cacgcttcat ggtcggcgca
     6121 gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa
     6181 agcgccccgt tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta
     6241 tacggacgac cggtattcag atggctgtag tacaccggga tctgcccgac agaacgcggg
     6301 aaggacattg gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca
     6361 ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct
     6421 ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct
     6481 ttcagcgccg taatcaagtc acgttggctt tgcggaatag tgatatcggt acggctggag
     6541 gcctcgtgcg ccatcccctg tgcttcaccg accacagcca ccaccacatc agattgtttc
     6601 gccgtctgca ccgcttcatc aatcatctct tgcggcgagc gcggatcgac tttgaccgct
     6661 tcttcatact gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg
     6721 gcatacagca ctttaccgtt ttcaccgacg gcatttttaa tcccggtcag tacggtcacg
     6781 gattgatcgg caacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc
     6841 agtggcccaa ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc gagacggttt
     6901 ttcagcaaca ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt
     6961 tcggcattgg tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta
     7021 aacaatccca tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctcttcc
     7081 atcgtcactt tgccggattt gatcaaccca ggcagatact tcgagtaata ctcgtcgctc
     7141 atactcatgt tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc
     7201 gtgccatgtt taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag
     7261 ccccactggt cgcgcagaac atctttcagc agccaggaat cggaggtggc tggcgtgccg
     7321 ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac
     7381 ggcggcatat aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta
     7441 ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga
     7501 tctgccgggc ttttaccctg catcgcttcc accatggttt tacccattgt tgaggtgaga
     7561 tacgtatctt cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg
     7621 accatcggtg cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgt
     7681 ccgaccgttt tcactgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac
     7741 accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt
     7801 aattccatca cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc
     7861 gccccaacct gaccgtcttt gatcatctcg cggatcgcct ctttcgggtt atccggaccg
     7921 acgctgatta agcgcagctg accaattttc tcatcaactg tcattttctt aagcagttcg
     7981 gtgacgaacg catcccgcgc ttcgggcgtt aatggatggt tgccgaataa atcatccgcc
     8041 agtgctggct gcagggccag actcaccgcg attcctactg aacatagcca tttcatatgg
     8101 atttttcttc ctctgttgcc cgacgaggca gcgaaaatgg tgcaaaaacc gtagtttgcc
     8161 ataagcatga tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt
     8221 cactcgctga attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca
     8281 aggagtggaa aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg
     8341 cccgtctggt cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca
     8401 agggcttccg ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg
     8461 aattgtggcg ggtgctgaaa gcctgtgtca ccgccgacaa aattattctg atgcaggctg
     8521 ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa cgactatgat cgcgatatcg
     8581 tgatcatcag caccctgcgt ctcgacaagc tgcacgttct tggcaagggc gaacaggtgc
     8641 tggcctatcc gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg
     8701 aaccgcactc agtgattgga tcatcgtgta taggcgcatc ggtcatcggc ggtatttgta
     8761 acaactccgg cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg
     8821 cgcgtataaa tgaagacggc aaactgacgc tggtgaacca tctggggatt gatctgggcg
     8881 aaacgccgga gcagatcctt agcaagctgg atgatgatcg catcaaagat gacgatgtgc
     8941 gtcacgatgg tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag
     9001 ccgacacgcc cgcacgttat aacgccgatc ctgatcggtt atttgaatct tctggttgcg
     9061 ccgggaagct ggcggtcttt gctgtacgtc tagatacctt cgaagcggaa aaaaatcagc
     9121 aggtgtttta tatcggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc
     9181 tggctaactt cgaaaatctg ccggttgccg gggaatatat acaccgggat atctacgata
     9241 ttgcggaaaa atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga
     9301 tgccgttctt ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct
     9361 tccgtccgca ttttactgac cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt
     9421 taccgccgcg catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg
     9481 cgggcgatgg cgtgggcgaa gccaaatcgt ggctggtgga ttatttcaaa caggccgaag
     9541 gcgatttctt tgtctgtacg ccggaggaag gcagcaaagc gtttttacac cgtttcgccg
     9601 ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg
     9661 cgttggatat cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga
     9721 tcgacagcca gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc
     9781 aggattacat agtgaaaaaa ggcgtggatg tgcatgcgtt gaaagaacag atgctggaac
     9841 tgctacagca gcgtggcgcg caataccctg ccgagcataa cgtcggtcat ttgtataaag
     9901 caccggagac gttgcagaag ttttatcgcg agaacgatcc gaccaacagt atgaatccgg
     9961 ggatcggtaa aaccagtaag cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg
    10021 catagtatcg ccatccgtca ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
    10081 tttgggcggc tttttgtttt ttatagctca acgctgctgc tggaacaggc atcactttac
    10141 cagtttcaat ccaggtacgc aggcggctgg catcggcaaa atgggtatat ttgccaaacg
    10201 catccatcac taccagcgct accggtttat tattgataac cgtacgcatc accagacaat
    10261 ggcccgccgc attggtaaaa ccggttttgg ttaactgaat attccagtta tcgcgataca
    10321 ccagatggtt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    10381 cttcccgggt agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt
    10441 tggttaagtc acgggcagtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc
    10501 gcgtgttgtt cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt
    10561 aaccaccggg ataatggtgt gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    10621 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    10681 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    10741 catccagcac aaccatcgcg gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    10801 ccgggtggtt cgagtagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    10861 aggcaatttc aggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    10921 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    10981 tgagcattca gatagtggtt cacgcgcaca ggttgcgcac cgccggagta aggatttact
    11041 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    11101 aaatgctgca ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa
    11161 acgataaccg tagccccata gtataacggt tagggcgagc agcacttcca gtaccagcac
    11221 gccaatcgcc agcgtcgaac tagagaagct aaggccttcc tctttgttga tattcaggaa
    11281 gctcggaata ccaaggtaaa gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag
    11341 cgcacacaac cagaccagtg gataaagcgc caccagaccg cttaaaaaca gcggagttgc
    11401 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcggat aattacgcgc
    11461 catccaccag atgacccgcc ccattaccgc aaccccagcc agcataacgc cataaaacag
    11521 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    11581 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    11641 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    11701 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    11761 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gctcatgtaa
    11821 tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga tttccagggt gtatgcttac
    11881 aggcaaccaa agggagacag actggcctat ggatctcaat acactcatct cacaatatgg
    11941 ttatgccgcg ctggtgatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    12001 cgttgcggca catcaggggc tattaaagtt cccgctggtg gtactttctg tggcgcttgg
    12061 cggcatgatt ggcgaccagg tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt
    12121 acgccgtttc tcgaaacatc aggataaaat tgagcgggcg cagaaactta tccaacgcca
    12181 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    12241 gctgattggt gccagccagc tgccgccgaa aatctttctg ccgctgaata ttctcggcgc
    12301 atttgcctgg gcgttgattt ttaccactat tggttacgct ggtggtcagg tgattgcgcc
    12361 gtggttgcac aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    12421 tctggtggtg ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggatcatca
    12481 ggcgtaaaac cattgccctg ggtaaggcgt tcgcgccgca tccgacatcc ggtgcaccat
    12541 gcctgatgcg acgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaataac
    12601 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    12661 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc tgagcacagc cacgctacca
    12721 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg
    12781 gcttcacgtc gctgtcatcc atgccattca ttggcgtggc gatcgcccca ggcgcgactg
    12841 cgttcaccaa aatcttatgc ctgaccagct ccagcgccat cgttttggtt aacccgccga
    12901 gcgcatgttt agcggctgtg taagcgctgg catccggcag cggcgtatgt tcatgtaccg
    12961 acgtaatgtt gatgatacga ccgccctgcc cttgtttcac catctggcgg gccgcaattt
    13021 gtgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    13081 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    13141 gccccagccg ttgaatgagt ttctccagcg cctgcgcacc ttctggcagt ttaccgagct
    13201 ccagctgcac gatctccgca cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg
    13261 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata
    13321 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgcaacc tgtgccatca
    13381 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg
    13441 taacccccgc ccaacgcacc agtgagttga atagaagcat ccagccactg cccttgcaat
    13501 aacaggccat tggcccgctc acgcagcgcg gggattctgg cttcgctgac gcgggaacca
    13561 acaatgatgc ccgcgttaaa gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    13621 tcaatttgcg cctgatgctg gtttttctcc gcaagtgtct gaacctgact ggctgcccgc
    13681 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    13741 tcggcttttg cgatattgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    13801 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    13861 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    13921 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    13981 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    14041 ggtttaagct tcaatgactt tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata
    14101 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    14161 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    14221 ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    14281 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    14341 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    14401 cagatatcga gatgccagcc cgccgttaag ccaaaagtac cgttggtgta ccacgggccg
    14461 gtcgtacctg cggccggatc gttcagagca aacggcccca ttaagccttc tgccgacatt
    14521 ttttgccgct ccatatccgc cgaaaagtcg atctgcggac catcctgagt ggcaactgcc
    14581 ttcgcctggg cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    14641 tgtagtgcat tgttaattaa tgaagtgagc tgattatcgt gatactccag ccaccattgg
    14701 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    14761 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    14821 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    14881 ggtaagaaac ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    14941 caatacatca gtagcaataa aacgacatac ccagaaaaat atagactaag taaatgatat
    15001 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    15061 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    15121 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa
    15181 ttaatgtcat caggtccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    15241 tgtcatgtgc attgcttcat ataatcactg gcgcaaggag cgcgcagggg gcggccaatc
    15301 gccgccgccc cctgcacccc cgggctctgg cgaacaaaat cgccgctgcg cggtgccctc
    15361 ggcttatccc ttacggctac cgggtcgggc gcgaggtaac atccctgtaa aacgcgccct
    15421 cagcccacct ccatgtgggc tgccccggcc ttcagggaac gcctcggcaa ttttgacgcc
    15481 accaaacaac cgtgcggcct attgataaag agctaacaca ttgtcaaaaa acatcactat
    15541 ggttttttag agtttctcga tatcaattgc ctgaatagcc cttgcaatat caggggaatt
    15601 attcaacacc cgaacatgct gaaataattc cgttgcttca tcgtattctt tacgcaaata
    15661 actcaaccac tgtttaatcc gcgcaacgtg atataacccg gtatcgccct gcttttccag
    15721 acgggtatat ttttgcagca aagcaaccac ctccggccac ggcattcgcg gttcgttata
    15781 ttttactacc cggctcaggt tgggaatatt gagcgccccg cgaccaatca tcactgcgtc
    15841 gcagccgctg atcgcaatgc attgttgcgc gctctgccag tcccagattt cgccgttggc
    15901 aatcaccgga atattcagcc gctggcgaat ctcgccaatc gcctgccagt caatatgctc
    15961 cgcgcggtaa ccctgctctt ttgtccgtcc atgcaccacc agctccgtag caccagcctg
    16021 ttgaaccgca tcggcgattt caaatttctt ctcaccgctg tcccagccca gacgcacttt
    16081 cacgctgacg ggcaaatgcg ccggtacagc ttcacgcatc gcttttgcac cctggtagat
    16141 gagttcagga tctttgagta acgtcgcccc gccgccgctg ccgttaaccg ttttcgacgg
    16201 gcagccgcaa ttgagatcca cgccccagga acctaattcc accgcacggg cggcgttctc
    16261 tgccagccat tgtgggaact gacctaacaa ctgaacgcgc accagcgtac cggatggtgt
    16321 ccggctggcg ttttgtagct cagggcaaat gcgatgaaag acttttaccg gcagcagttg
    16381 atccaccacg cggacaaact cggtgatgca cagatcgtag tcgttaactt cggtcagcaa
    16441 ttctcgcacc agagagtcaa gcacaccctc catcggagcc agtaacacac gcatatcttc
    16501 acccggaaaa aaatgaggcg ctatgttagc gcctatggtc aacggattaa aggtctgcaa
    16561 agagggattg ctgcaaactg taatccctct ctatgcttga tgtatgcctg agttcgttgc
    16621 gcattgcgta atgccgggga gcgaaaaaat aagcgctgat ttcttaatgt gatcggtagc
    16681 acgttttacg aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa
    16741 gacactgaac attatctggc aatatttacg cgctttcgtc ctgatttatg cctgcctgta
    16801 tgcaggcatt ttcattgctt ccctgctacc ggtaaccatt ccgggcagca tcatcgggat
    16861 gctgatcctg tttgtcctgc tggccttgca aattcttccg gcaaaatggg tcaatccggg
    16921 gtgctacgta ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat
    16981 gcaatatttt gatttgctcc gcgcacagtt tggcccggta gtggtttcct gtgcagtcag
    17041 tacgctggtg gtttttctgg tggtgagctg gagttcacaa ctggtacacg gtgaacgtaa
    17101 agtcgtaggt cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg
    17161 acgttgattg tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt
    17221 aacccgttgc tggtagcaat ggtggtgatc attccttttt taatgctgac cggcatctct
    17281 tacgacagct actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc
    17341 gcgctggcct atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc
    17401 atcaccatct gttttatcgg cagcgtggtt gcgatggtga cggggacttc cgtggcattg
    17461 ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct
    17521 attgcaatgg cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtg
    17581 attttcgtcg gcatcctcgg cgcggtattt ggccatacat tgcttaatgc gatgcgtatt
    17641 cgtaccaaag ctgcgcgcgg tctggcaatg gggacagcct cgcacgccct cggtacggcg
    17701 cgctgcgccg agctggatta tcaggaaggt gcatttagtt cgctggcgct ggtgttatgc
    17761 gggataatta cttcgctgat cgcaccgttc cttttcccga ttattctggc agtaatgggc
    17821 taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga
    17881 gattcagatc acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta
    17941 tgaggcaacg ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg
    18001 caatctgcac tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag
    18061 caagtctcat cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta
    18121 cttccactgg cggcggcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt
    18181 gcgcgcggtg tgagcggaac ctggtatttc ggtgccaata tggaatttat tggtgcaaca
    18241 atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa
    18301 aaagcgcttg cagccatcac cgttaactat acgccttgtg gtcactgccg tcagtttatg
    18361 aatgaactga acagcggtct ggatctgcgt attcatctgc cgggccgcga ggcacacgcg
    18421 ctgcgtgact atctgccgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg
    18481 atggacgaac aggatcacgg ctatgcgctg acgggtgatg cgctttctca ggcagcgatt
    18541 gcggcggcaa accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa
    18601 tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact
    18661 ctgccaccgt tgcagggggc gttaattctg ttgaatctca agggttatga ttacccggat
    18721 atccagcgcg cggttctggc agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc
    18781 tccgcaacgc tgaaagctct cggctgtcac agtatcgacc gcgtgcttct cgcttaagcc
    18841 tggtgccgga tgcggcgtga acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa
    18901 ttcccggcaa acagtttgcc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga
    18961 aattttcctt agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc
    19021 tgatcggctt gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg
    19081 cgccttatat ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggc gtggtgctcg
    19141 gcacagcaaa atattatcgt actggcgtaa ttaatcagta ttatcgctac cgcattcaag
    19201 gagcgattaa tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg
    19261 cattgcaaag ttataatgag ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg
    19321 acccatcaga tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta
    19381 cacgcaaagt gttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc
    19441 gggcattatt tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac
    19501 cgaaagatat gctgtcagta cgtattcgcg aatttgccgc ccgtttcggt gcgctggctg
    19561 acctttatat ttttagacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta
    19621 tgcaccaggt gccggaagat gcgcaggggt atcccgccgt cacacccgaa cagttacttg
    19681 aattacaaaa gaaacaagga aagtagttat ggatgtacag cagttttttg tcgttgccgt
    19741 ttttttcctt atcccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc
    19801 gattgataaa cgggttaaaa atgcaccgga accggtgtat gtctggcgag caaaaaatcc
    19861 cggactcttt ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat
    19921 gattgtttat cttattttct atcgttaata ttttctcttt tctgttaatt attgagaata
    19981 attattactt cactcaataa gctgcggata taacattctg aaccgcagct atttgtgaat
    20041 cctttcacag tttaaatttc cccacacgct tagccttaat atcagcacgc aatatttatt
    20101 tagcgttcgc cttaattact catagcatta aggaagatca catgccgcaa caaaattatc
    20161 tggatgaact cactccggct tttacgcctt tactggcgat taaagaagcc tctcgctgtt
    20221 tattatgtca tgacgctccc tgtagtcagg cttgcccggc acagaccgat ccggggaaat
    20281 ttattcgctc aatctacttt cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata
    20341 atgccctcgg tgccgtttgt gccagagtgt gcccgacgga gaaattatgc caaagcggtt
    20401 gtacccgtgc cggtgttgat gctcccattg atatcggccg cttacagcgt tttgttactg
    20461 attttgaaca acaaaccggg atggaaattt atcagcccgg tactaaaacg ctcggcaaag
    20521 tcgcgattat tggcgcaggt cctgccggat tgcaggccag tgtgacactg acaaaccagg
    20581 gttatgacgt cacgatctat gagaaagaag cacaacccgg tggctgattg cgtaacggca
    20641 ttccgcaatt ccggttaccg caatcagtac tggatgcaga gatcgcccgt attgaaaaaa
    20701 tgggcgtgac cattaagtgc aacaacgaag tcggtaacac actcaccctt gagcagctga
    20761 aagcagaaaa ccgcgcggta ctggtcaccg tggggttatc aagcggttcc gggctaccgc
    20821 tgtttgagca tagtgacgtt gagattgccg tcgacttctt gcaacgtgca cgacaggcgc
    20881 aaggcgatat cagcattcca caaagcgcat taattatcgg cggcggtgat gtcgcgatgg
    20941 acgtagccag cacgctgaaa gttctcggct gtcaggcggt aacttgcgta gcgcgtgaag
    21001 agttagatga gtttccggca agcgaaaaag agtttaccag cgcccgggag ctgggcgttt
    21061 cgatcatcga tggattcacg ccagtagccg tggaaggcaa taaagtcacg tttaagcatg
    21121 tacggttatc gggcgaactg acgatggcgg cagataaaat tattctcgcc gtcggtcagc
    21181 atgccagact agatgccttt gcggagttag agccgcagcg taacaccatc aaaacacaaa
    21241 attaccagac ccgcgacccg caagtctttg ctgctggcga tattgttgag ggagacaaaa
    21301 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
    21361 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
    21421 caacccgttc tgcctctctt cttcgccggt aggcaactgc tatgagatgt gtgccaaagc
    21481 ctacgacaca ggttggggcg gtgtggtgtt taaaacgatc ggctttttta tcgccaacga
    21541 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
    21601 tatggagcag attgccgagc atccgctgga ggagaatctg gccgccctgc gtcggctgaa
    21661 agaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
    21721 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
    21781 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
    21841 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
    21901 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
    21961 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
    22021 tatgccgatc gttaacggaa aatcaagtat ttccggatat tccggtaaag cggtaaaacc
    22081 gatcgccctg cgcttcattc agcaaatgcg cacccatcca gaactgcgcg atttcccaat
    22141 cagcggtatc ggcggcattg aaacctggga ggatgcggcg gagtttttat tgctcggcgc
    22201 agcaacgtta caggtcacca ccggcattat gcagtacggc tatcgcatcg tagaagatat
    22261 ggcgagcggg ttatcgcatt atctcgccga tcagggattt gattcgttgc aggagatggt
    22321 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
    22381 tccccgtatc aatcttgaca aatgtgttgg ctgtggacgc tgttatattt cctgttacga
    22441 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
    22501 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
    22561 ggaagtgaag tttaagaaag gcgagaaaga acacccggta acgttgtaga acccgcgtca
    22621 catccggcat ttggttcctg ttgccggatg cggcgtgaac gccttatccg gcctacaaaa
    22681 ccacacagat catgatgtta tgtgcgttta tgaccgaatg cggaccacat tcacatcatt
    22741 tcttacgcgc gtatttcagt gaatccagcg ctacggcgaa gataataatc gcccctttga
    22801 tgatgtactg ccagtatggg tttacgccga tatacgtcag gccatagttg atgacggtaa
    22861 aaataatcac cccggtcacc acgccaatca ccgtccccac accgccgctg aacgatacac
    22921 cgcctaccac gcacgccgcg atagcatcca gctcatacat aaagccgagg ttgttggtgg
    22981 cagagccgat acgtccggct tctaacatcc cgccaaaggc atagaacacg ccagacaacg
    23041 cgtagatcat cagcaggttc aggccgacgt tgacaccaga tacttttgcc gcttccgggt
    23101 taccgccaat ggcaaaaatg ttcttaccga agcgggtttt gttccacaac acccagacga
    23161 acgccaccgc aatcaacgcg tagaaggtga tgtaagagag acggaaactc cccagcgcga
    23221 caaagccctg agcaaaggta gagaagccac tgtcaaaacc agaaattggc gacgccccga
    23281 caaagtcgta atagagcgag ttgatgccat agacgatgat catcgtgccg agcgtggtaa
    23341 tgaacggcgt cacgttgaga taagcgataa tcagaccgtt gatcaaaccg atcaccgcac
    23401 caatggcaca gacaatcaga ataaccagcg caatcggcat cgtcgccatt tccgggaaca
    23461 ctttgttggc gttatccatg gactgcaata atgtcgcagc caccactgcc gccagcccta
    23521 cctgacgacc agcagaaaga tcggtcccct gggtgacaat taaccctgcc acaccgagcg
    23581 cgataataat acgcaccgat gactgggtga gaatattact taagttcaac agacttaaaa
    23641 atgttgggtc ctggaaaata ataatcgcca gcaaaactaa aagaacgacg taaataccgc
    23701 cctctttcag gtaagtaaga aaacttttct tatttaacgc actcatggga agcccctaat
    23761 cttaaaggtg caaagacgca agacgcagaa tttcgttttg cgttgttgtt tttgtatcga
    23821 caattccgga aacgagacca ttgctcatga ccagaatacg gtctgttatc cctaacaact
    23881 caggcatttc agaggagata ataataatcc ccttgccttt cttcgccagt tcggcaatta
    23941 actgataaat ttcaaacttc gccccgacat caataccgcg cgtcggttca tcgagcatta
    24001 atatttctgg ttgcgttagt agccagcgac caataatcac cttttgctga ttaccaccgg
    24061 agagcgaacc aatttgcgtc cgatgacccg gcgtttttac ccgcatcgaa tcaatcaccc
    24121 actgggtatc gcttttcatc cgcgagttat ccagtaaacc aactttattt ttgtagttgc
    24181 gaatattgga aattaacgag ttaaaaccaa tatccagata ggcataaatt cccgttgagc
    24241 ggcgctcctc agttaccagt gcaaatccgt ggtttatggc ttcgttggca ttatggttat
    24301 tgatctgttt gccgtgcaac gtaatggtgc cagccgattt ctcgcgaata ccaaataacg
    24361 tctcaacaat atcggtacgt ttcgccccca ccagcccggc aataccgagg atctcccctt
    24421 tatgcagatc aaacgagaca tcgcgaatcg acggctggcg cagtgacgtt aggttacgca
    24481 cctcgaggat gacttcgccc ggcttgtttt ctttgtcagg aaaacgctga ttaagagaac
    24541 gcccaaccat catggcgatg atcttgtcca tcgtcagtcc tgccagcggt tcggtggcga
    24601 tccactgacc gtcgcgcaat acggtaactt catcacataa ctggaagatt tcttccattt
    24661 tatgcgagat ataaacaata ccgcagccgc gctcttttaa tttacgaata atagtgaaca
    24721 gatgattgac ctctttttcg gttaacgaag aagtcggttc atccataatc acaattttcg
    24781 cgttatagga aaacgctttg gcgatttcga tcatctgcat ttgcgaaacg gataatgtgc
    24841 cgacgcgcgc acgcggatcg atatcaatat ccagttcatc aaaaattgct ttggtttcgc
    24901 ggtacatttt gtcctgatcg acaaacatgc ctttggtggg atatcgcccc agccacatgt
    24961 tatccatcac cgaacgttgt aataccaggt ttaactcctg gtgtaccatc gaaataccat
    25021 tttccagtgc ctctttggcg gaatggaaat cgatctcttt accctggaat taaatggtgc
    25081 cggagtcttt ttgataaata ccaaacaggc attttaataa tgtcgatttt cctgcaccgt
    25141 tttcccccat taatgcatgg atagaatgtg gccggacttt taaattaacg ttatcaagtg
    25201 ccttaacacc aggaaaggac ttgttgatac cgctcatttc caacaagtat tccccggaag
    25261 acggagtcgt tgagctgacc atataatttt accttgttgg ccatacaata agggcgcagt
    25321 aaacgactgc gcccaatcag tcttatttct tgctgaattc agccaggttg tctttatcta
    25381 cgccaacata aggtacgcgg accactttgt tgtcgatttt ccagttggtg ccatcagccg
    25441 cacctttgcc atcggccagg tttttcgcca gatcaaaggt cgctttcgcc tggttgttag
    25501 catcgttcag tactgtgccc gccagtgcac cggatttcac cagcgccagc gcttctggca
    25561 gcgcatcgac gccaaacacc ggaatgctgg acttgttgtg tgctttcagt gcttctaccg
    25621 cgcccattgc catcgcatcg ttgttggcga taaccacttc gattttgttg gcgttcgggc
    25681 cagacagcca ggcgtccatc ttatctttcg cctgagcggt gtcccacatt gcggtatcta
    25741 actgtaactg ttcagttttg atgcctttat cgttcaattc tttaatcacg taagtggtac
    25801 gtgcttctgc atccggatgg cccggttcac ctttcagcag tacgaactga atctgaccgt
    25861 ctttgttcag atcccaaccc tgattcgccg cccagtgttt agcaatcaaa tcgccctgaa
    25921 taatgccgga ctctttggag tcagtgccaa cgtagtaggc tttgtcgtag ctatccagcg
    25981 ccttacgaga cggttctttg ttgaagaaaa ccaccggcac gttttgccca cgcgctttct
    26041 caatcaccgt acccgcagct gccgggtcaa ccaggttgat tgccagtgcc ttcacccctt
    26101 tcgccagcaa tacgtcgatc tgatcgttct gcttggactg gtcattctga gaatcattca
    26161 tcagcagctg aacatctggc gcggctttcg catcttgctc aatagccttg cgcactacag
    26221 acataaagtt atcgtcgtac ttatagattg ttacaccaat gcgagtatca gcagcgtgtg
    26281 cagcggcacc gaataacatg ctggccatca cagcagacag ggttaacacc ttcttattca
    26341 tggtatctcc ggtttttctt atgcagggta gtgcttgaga taaatgctcg gcggggcagt
    26401 agagttaatg aagtgttact gaacgccgaa gctcactttt ttaaattcgt tcttccatgc
    26461 ccggtaacgc tccagaaaac ggctttaatt gttgttatga cgctattacc tcggtgaaag
    26521 tgattattca ctgttacata cgggttacaa cgttaaaacg gtgcaatcat agctatcaca
    26581 ttgttaagat actgtgaaat cactcacaga ttgaaagcgg ttacatcgcc tgatttgttg
    26641 agttagtgat cgccgccgca ttctggcgcg ttgcgacaga atggcgacgc actaacgtcg
    26701 gcataaaaca gtggctggca cgaggatcaa tattgcctgc tgccccctgc aaggccagtt
    26761 cggtggctaa tttagccatt gaagcaatgg gataacgcac ggtcgttaat tgcgggtcgg
    26821 tgtaacgggc gatgggaata tcatcgaaac cgatgattga gagatgtaac ggaatcgcaa
    26881 tgccattatc ttttaatgct gtcagtgcgc cagcggccat attgtcgtta taagcaaata
    26941 tagcggtaag ttgtagattg cgccccagca gttcaaccat cgccgcctca ccgcccggca
    27001 tgtccggcgt accagtgcca atccagcttt ccggcggaat aatatcctgc tctttcaacg
    27061 cactcatcca gcctgcttta cgcatggcgt catcttcaat gccgtgactg gaagaaagat
    27121 aaccaatacg ttgatgaccg ttattcagca acatgcgcgt cgccattcgg gcaccgctga
    27181 gattatccag gcaaacgcaa cgatgggcgt accccggcac aacgcggttg attaacacca
    27241 taccgggaat gttatccata aattgcgcca gttcatcgtc actcaatgct tttgagtgaa
    27301 caatcaacgc attacaacgc tggcgaatta acacctcaat ggcgtgacgc tctttttccg
    27361 cttcatgata gctattgccg attagcacgt atttctgatg ctgctgagcg actagatcca
    27421 ccgcttttac cagcgcgccg aaaaacgcat cagaaacgtc catcaccacc acgccaatgg
    27481 tgtcgctaac ctgagttgcc agcgcctggg cattggcgtt tggccgataa tccagctcac
    27541 tcacggcttt cattactgct tcacgcgtgt cggcactgac cagcgtgctg ttattgagca
    27601 cccgggaaac cgttgccaca gagacgccag cctaacgcgc tacatcacga atggtgatca
    27661 tattcactac ctgttatgag attgcagtaa atgactgctt gctggcggct attttgtcag
    27721 cactgaatac aggacttcgt gaatcgagtc acagcaatgg aaacggttac agccgttttg
    27781 ttaacgaatg tgatccagat cgttatcttg atgttttaga tattgccgtt ccggcagcac
    27841 gcaaagttaa ctgacgccag agccattcca ccggcccctg gcggaagtaa cgcagccaga
    27901 taacagagaa gaggatattc gccagccata ccggaataac aaacgccagc agctccaggc
    27961 ggtcaaaatg cataaacaaa ccgaggtggt aaaaaagcat ggtacaaatc agcgtttgca
    28021 atagatagtt ggtcagcgcc atccgtccga cgcaggcgat cgcaagcacc agcttaaagc
    28081 ggctcaattg cggccagaag ccataaaaca gcgacgcata gccaatcgcc tgaaacggcg
    28141 cactcagttc ccgcggcatt tgaagtaaga aggcacacca gcgatacgcc cagtccagct
    28201 gccattgcag ggcgatggca ggaaggttaa tggtcacccc aatcgccacc agcacaaaac
    28261 cagtacgacg atagtgacgt aagctgaact gccctttcag ccagccgctg cgcatcagcg
    28321 ccgcaccaat gagcatcatc cctgccagtt gccagccata ctgtgcgccc agtgccagta
    28381 aactgttgcc aacaccatcg gcacgattac tgatcgcttc aacgccgccg tgaagcttcc
    28441 agtatttttc atacaaaata gccgatgcat ccggcgtcca ggtgcggctg gtctggctgt
    28501 cggaaatcag ccccaataac agcaaaacgc caagccccac cagataaagc atgacgcctg
    28561 tattaaacag gctttttacc gatggcgcat cgcgcaccag ccgccagcag attaagccca
    28621 ccagcccgta agccagcaga atatcgccgt cccagaacaa taaaccgtga ataaaaccca
    28681 gcaagactaa cagcgttaac cgcgactgga tccagcgtct gccacggggc agcaacattt
    28741 gcaggcccgc accaaacagc agcgcaaaaa gcgtgaggaa tttcacctgg ccgatgagat
    28801 cgagaaatgc ccaggtccat gcatcctgcg gcgtaatagc gccgtaccag gcgggattga
    28861 gataagccgc ctttggtagc ccaaaggcgc tgatgtttaa tagcaggatc cccagaatgg
    28921 cgacgccgcg aacaaaatcg agcgttacgt tgcgctccat ggttcctgcc ttttaatcag
    28981 ttgtgatgac gcacagcgcg cagaaactcg tggcgcgtat tctgactgga tttgaacaat
    29041 ccaccaagag aggtcgttgt tgtggcactg gttgcatcgc ggatgccacg cgctttcacg
    29101 cagtaatgca ccgcatcaat ggagacagcc acgttattgg tgcccagcag cgtttgtagc
    29161 gcaataagaa tttgctgcgt cagacgttcc tgcacctgcg gacgctgggc aaagaactgc
    29221 acgatacggt taatttttga cagaccgatc accgaatctt tcgggatata agccaccgtc
    29281 gctttgccat cgatggtaac aaaatggtgt tcacaggtac tggtcagagt gatatcgcgc
    29341 acggtgacca tttcatcgac cttcattttg ttttcaatga gggtgatttt cgggaagttg
    29401 gcgtaatcca gaccggagaa aatttcatcg acatacattt tagcgatgcg atgcggcgtt
    29461 tccatcaaac tgtcatcagc caggtcgaga ttcagcagct gcatgatttc ggtcatatga
    29521 ccagcaataa ggcttttgcg cgtttcgtta tccatttcat gcacgggcgg gcgcagcggt
    29581 gtttccagtc ctcgcgcaac taacgcttca tgaaccaggg ccgcttcttt actgagtgat
    29641 ggcatttatg atttctcctg caggtgtgac gcctccgccc tgcgtggggg caaagttatt
    29701 aagctgattt acagcctgat tattgtgcgt gaggcggcgc acataatcca gtattcacag
    29761 cgataattat tgtaattgcc gctgactttc atcagcagat gttaaaacat cgttatgcaa
    29821 atacggaagt gaaagttact cacagcacat tgaataaacg gtatgatgaa gaaattgcaa
    29881 acaacacaac aaggagccac gcatggaaat gctcgaagag caccgctgtt ttgaaggctg
    29941 gcagcaacgc tggcgacacg actccagtac cttaaactgc ccgatgacgt tcagtatctt
    30001 tctcccaccc cctcgtgatc acaccccgcc accagtgctg tactggcttt ccggattaac
    30061 ctgcaatgac gagaacttca ccaccaaggc gggtgcccag cgcgtggcgg cagaactggg
    30121 gattgtactg gtgatgccag acaccagccc gcgcggcgaa aaggttgcca acgacgatgg
    30181 ctacgattta ggccagggcg caggctttta tcttaatgcc acgcaaccgc cgtgggcgac
    30241 gcattaccgg atgtatgatt atctgcgcga tgaattaccg gcgctggttc agtcgcaatt
    30301 taatgtcagc gaccgctgcg ccattagcgg tcactcaatg ggtggtcacg gtgcgctgat
    30361 tatggcgctg aaaaatccgg gtaaatacac cagcgtttcg gcctttgcgc caattgtgaa
    30421 tccgtgcagc gtcccgtggg gaatcaaagc gtttagcacc tatttaggtg aggacaaaaa
    30481 tgcatggctg gaatgggata gttgcgcgct gatgtatgcc agtaacgcgc aggatgcgat
    30541 cccgacgctt atcgatcagg gcgataatga tcagtttctt gccgaccagt tgcaacctgc
    30601 ggtattggca gaagccgcgc gccagaaagc gtggccgatg acgctgcgta ttcagccggg
    30661 atatgatcac agttactact tcatcgcctc ttttatagag gatcacctgc gcttccatgc
    30721 gcagtattta ctgaagtgaa agtccgcccg gttcgccggg catcttctca tcagaagcga
    30781 taatccactg ccataaagta acgacgtccg tcttcgttat agctgtagtc gtcacgactg
    30841 agatccttgt cgccaaggtt cagcacgcct gcgcgcagtt tgacgtcttt agtcacctgc
    30901 caggccgcgc cggtattcca gatggtataa ccgcccggtg ttttagccgt cgcgctgtcg
    30961 gcgcgtttct gcccggtata gtgaccagaa acatagaatg accagtcttc cagcgccagc
    31021 ggtttccagt ccagcgtacc gttagcagta tggaacggca gatcggatag cggtttgttt
    31081 tcgccgttgc tgacatcacg accatcgttg taggtgtagt tgatcgacag tttccattca
    31141 tcgttgaacg gaattttcag ttcggtttcc acgccctgaa tacgagcttt gttaacgttg
    31201 tagtagctaa ataccggtat gcgccgtccg ttagcgcccg tctcaaaacc aacaaagttt
    31261 tggtagcccg gtgcagcgtt gacgtcagac gtacggctga tgctgatacg atctttcaca
    31321 tcgttacgga aaacggtaac gctggattca accccttcca gccagccttc ttcacccatg
    31381 tagtaaagcc ccagctccca actttcgctg gtttctggtt tcagatccgg gctacccaca
    31441 atcttacatg cgccacggca ggaattgctc gtccagtcag ggctaagttg caacagagaa
    31501 ggtgctttaa atgccgtcgc ccagccccct ttcaccgtta cggtgtcggt ggcgttataa
    31561 accaggtagg cacgcggact ccagtgttca ccgtaggttt cgtgatcgtc catacgcacg
    31621 ccggtcgtca gcgccagcgg ctcgaagatc cgccattcat cttccacaaa cagcgcgtac
    31681 tggctggcag acgttttgga gctggttccc ccggtcaggt tcaccgcatc gctaagtttg
    31741 tcgtgacgcc attcaccgcc aaccgtgaga aactgattaa tcgccgtcag cggcaacgtg
    31801 tatttgccgt cgaccgtatt gctttcggaa gttatcgggc tgctgttgcc agggtttttg
    31861 ttctcgactt tctcaccgta gtatttcagt tcgctggtgc cgtaatccca acgcccatta
    31921 tggctgacgg agtagttctg gcgttccagg cggtttttgt ccagcgagtc ggaatcacga
    31981 tcctgacggt cgaaaccgta tccggcagta aaatcgtgat tttgattcgg tgtccaggca
    32041 aattcgacat tgccgtcgcg gctggagaat ccttcaatac gcggcgtttc tccggtatcg
    32101 gtggtcgttg agttttgcgg gtcatccttt tcacgttttg ccaggctgcc gtaagctttc
    32161 attcccagca caccatcaat taatggtcca ctggtaaaga actgaccgtt ataggtatca
    32221 ccgcgatcgc gatgttcctg aatggtggta tcgacggtaa cggtacccga ccatttctga
    32281 ccgatttttt tggtgatgat attcactaca ccgccgagcg catcggaacc gtacagcgac
    32341 gacatcgggc cacggaccac ttcaatacgt tcgatggaat cgaccgggat ccagttcaga
    32401 tcgaaatcat tgtggcggaa gacggcattg cgggagttca cgcgtttacc gtcgacgaga
    32461 atcagggtat agctgctgtc cagaccacga atactaacgc ccttacggtt atccccttcg
    32521 ttcgtcagtt gtacgccagg cacttctttg aggacatcct tcagattctg taccggtttt
    32581 cgctgcagat cttcctgggt aatgacgctg atactggcgg gtgcatcttt aaggttttgt
    32641 tcaacggaag atgcagtgac aaccatcgtt tcgccatcgt catcgaccgc taacacaggc
    32701 catacacaag aaatagcgga caaacacagc ccgacccgta cgaaagggtt caacctaaac
    32761 attccatatc tccatgaggt aactacgaaa ataaaatggg tatcgctcac atcttcttta
    32821 cgtccccttg cgtacggcag catcgcggtg gacttatttg tatgcaggtg atcatcccag
    32881 aaagccggac cagcctctga tttgtcagcc ttttttgatt gtagcgtaac gataatgcaa
    32941 acgataacaa ttatcaatcc aaatgttaaa ttttatatcc gcatgatttg tcagggaaat
    33001 ggaattgaaa aagccctctc ggttgagagg gcttagcaag gaagggagga aacttatttc
    33061 ttatcgttct gcgggaactt catttcgctg tagcgtacga agtgagttcc tttaatcagc
    33121 ttgtagccga accaaataat caggaacagc gggataccaa tatacgttgc cgctacgccg
    33181 ccccagtcaa tggtatcttt caggaacgct tcgtagttct ggcccaaagt gataatcaga
    33241 cacagaatga atgcgaagat cggccccagt gggaagaaac ctgaacggta cggcagatcg
    33301 ttaatgtcgt gtccctgcaa tacgtaaccg cgacggaagc gatagtggct aatggcaatc
    33361 cccagccagg cgataaaacc cgtcatcccg gaggtgttca gcagccacag gtataccgtc
    33421 tggttgccaa acatggaggt caggaagcac aaaccggcaa tcaccgtcgt cgcatacagc
    33481 gcattacgcg gcacgccacc acgcgacaat ttagcgaaaa tgcgcggcgc tttaccgtca
    33541 cacgccaggg tgtacagcat acgagtagac gcatacatac cggagttacc tgctgacagc
    33601 accgccgtca gaataactgc gttcatcacc gccgccgcag agagcagacc cgcgtgctgg
    33661 aacaccaagg tgaacggact gacgctgatg tctttaacat cgttacgcag caggctcgga
    33721 tcggtatacg gaataatcag gctgataatc aggatcgcga acacatagaa caacaggatt
    33781 cgccagaata cctgacgtac cgcgcgtgga atgtttttcg ccggatcttc ggactcgcct
    33841 gcagcaatac cgatcagctc ggttccctgg aaagagaagc cgacaatcat agctacgccg
    33901 atcatcgccg caaaaccacc agcaaacggc gcttcgccga ttgtccagtt gctccagccc
    33961 gcaggctgcg cgcctttgaa gataccgata atcatcagca cgccaacgat gataaagaca
    34021 ataactgtcg tgactttgat cagtgaaaac cagtattccg cttcaccaaa gccacgaact
    34081 gagatgtagt tcagcaggaa gataacgccg aggaacaacg cactccagat ccagcccggt
    34141 gtatccggga accaccagct catgaccagc tgtgctgcaa ccaagtcaac ggcaatagtc
    34201 accgcccagt tgtaccagta gttccagccc agcgcgaagc caaagccttc ttcaacatag
    34261 ttctgaccgt aagtggcaaa cgaaccggaa accggcatat atgcagccaa ttcaccgaga
    34321 ctggtcatca ggaagtaaac catcaggcca atcagcatat acgagagcaa tgccccgccc
    34381 ggacctgcct gagaaatcgt tgcgccagag gcaacaaaaa gacctgtacc gatggaaccg
    34441 ccaatggcaa tcatcgtcag gtgacgcgcc tttaattcac ggcgtaagcc cggcgcttct
    34501 gtagttttag tttcggaacc catacaaaaa tgctatccat cttaaaaatg aggcgcgatt
    34561 gtagcagacg attggcaatc cttccggcag aaatacacgg ttataagaca ccttcatgat
    34621 cgcccaggga ttataagtaa agcagccaat ctcttttctg gcgaatgcat gctgagggcg
    34681 ctgcgtcacg aaatcgacac agcaccagca tgttcttgta cagcacctta acgcggtaca
    34741 ttcgcgggat cgcaatagtc cagaaagcgc cgtagcgcgt tggaaaggtg tttttgccga
    34801 tgatgtatac gccacaacgt acgcatcagg cgcgggagag ggaccgcaac ttcacttaat
    34861 gtgcctgcct gcaattgatc ttcaatcaca cgtcgcgaca ggcaactaat tcccaaccca
    34921 tgacgcaccg catgtttgat tgcctcggag ttacctaatt ccatcgccat ctcaaacttc
    34981 ggtaaatgtg acagcaacag ataatcgaca atctcccgcg tgccggaacc gcgttcacgc
    35041 aggatccacg gcgcagcggc cagctgttct aaggtgaccg gaccacgagc caacggcgaa
    35101 gtcggcgcgg cgaaaaccac cagctcgtct tccagccacg gttcagaaat gatttcagtg
    35161 ctgtggcacg gtccttcaat aaagccaata tcaacgcgga aatccagcac cgcttgcatc
    35221 acgtcctggc tgttcccaac gctaagttca atcggcaact gcggataatc atggcgataa
    35281 cgggcgataa ctgcaggcag aatgtagtta ccgatggtac tactggcata gatacgaatc
    35341 gcgccgttgt cttcgcgaaa cagttgttcg atttcaaccg cctgttcaag caatgccaac
    35401 gcacgcggat agagcagccg cccgtgttca ttaacaacca gtcttttccc cacgcgatca
    35461 aacagttgca cgccaagctg cccttccagg tcggtcaagg ctgcgctcac tgctgattgc
    35521 gacaacgcca gcatcaccga cgcctgggtg gttgatccac ttttcaatac ttctgcaaaa
    35581 acttccaact gccggagggt gatgtgcata gtcgcttacc acttataaag attaattata
    35641 aatatataat caattttatt tttaaaccag ttagtcgtaa ccttatgccc ggttaaagga
    35701 gagggttatg acgaatatca ccttacagaa acaacatcgt acactgtggc attttattcc
    35761 ggggttagcc ctgagtgcag ttatcaccgg ggtcgccctg tggggtggtt ccattcccgc
    35821 ggttgcgggt gccgggttta gtgccctcac cctcgcaatc ttgttgggga tggttttagg
    35881 caacaccatc tatccgcaca tctggaaaag ctgtgacggt ggtgtgctgt ttgccaagca
    35941 atatttatta cgtctgggta tcattcttta tggcttccgt ctgacgttct cgcaaattgc
    36001 cgatgtcggt atcagtggga tcatcattga cgtgttgacg ctgtccagta ccttcctgct
    36061 ggcttgcttc ctggggcaga aagtgtttgg tctggataag cacaccagct ggttgatcgg
    36121 tgccggtagc agtatctgtg gtgctgccgc ggtactggcg actgagtcgg tagtgaaagc
    36181 ggaagccagt aaagtaaccg tggctgttgc aaccgttgtt atcttcggga ccgtcgcgat
    36241 tttcctctac ccggcgatat atccgctgat gtctcaatgg tttagtccgg aaactttcgg
    36301 tatctacatt ggttctactg tgcacgaagt ggcgcaggtg gtggcggcag gtcatgccat
    36361 cagcccggat gcggaaaacg cagcagttat ttccaaaatg ctgcgcgtga tgatgctggc
    36421 tccgttcctc atcctgctgg cggcgcgtgt taaacagctg tctggggcga acagcggcga
    36481 gaaaagcaaa atcactattc cgtggtttgc catcttgttc atcgtcgttg ccatctttaa
    36541 ctcgttccac ctgttaccgc agagcgtggt gaacatgctg gtaacgctgg ataccttcct
    36601 gctggcaatg gcgatggcgg cgctgggtct gaccactcac gtcagcgcgc tgaaaaaagc
    36661 tggggcgaaa cctctgctga tggcactggt actgtttgcc tggctgattg ttggtggtgg
    36721 tgcgattaac tatgtgattc aaagcgtcat cgcataaacc actacatctt gctcctgtta
    36781 acccgctatc attaccgttt tcctccagcg ggtttaacag gagtcctcgc atgaaataca
    36841 ttggagcgca cgttagtgct gctggcggtc tggcaaatgc cgcaattcgc gccgtcgaaa
    36901 tcgacgcaac cgcgtttgcc ttgttcacca aaaaccaacg tcagtggcgt gccgcaccgc
    36961 tcacgacgca aaccatcgat gaattcaaag ccgcctgtga aaaatatcac tacacatcgg
    37021 cgcaaattct tccccacgac agttatctga ttaacctcgg acatccggtc actgaagctc
    37081 tggaaaaatc gcgcgatgcc tttatagatg aaatgcagcg ttgcgaacaa ctggggcttt
    37141 ctttgctcaa cttccaccct ggcagccatc tgatgcagat ttcagaagag gattgccttg
    37201 cgcgtattgc cgaatccatc aacattgcgc tggataaaac tcaaggtgta acagcggtga
    37261 ttgaaaacac cgccggtcag ggcagtaact tagggtttaa attcgaacat ctcgcggcga
    37321 ttatcgacgg cgtggaagat aaattccgcg tcggcgtctg cattgatacc tgccatgctt
    37381 tcgctgccgg gtatgatttg cgtactccag ccgaatgcga aaaaacgttc gctgattttg
    37441 cccgtattgt cggctttaag tatctgcgcg ggatgcacct taacgatgcg aaaagtacct
    37501 ttggcagccg cgttgaccgc catcatagcc tcggtgaagg caatatcggt catgatgcgt
    37561 tccgctggat catgcaagac gaccgtttcg acggcattcc gctgatcctt gaaaccatca
    37621 acccggatat ctgggcagaa gagatcgcct ggctgaaagc gcaacaaact gaaaaagcgg
    37681 tggcctgatc gatgaacgac agggaaaagc agattcttaa aattttacgg cgtaacccgc
    37741 tgattcaaca gaatgaaatc gccgatattc tgcaaatcag ccgctcacgt gttgccgctc
    37801 atattatgga tttgatgcgc aagggtttga ttaaaggcaa aggatatatc cttaccgagc
    37861 aggattactg tgtggttgtg ggtgcaatca atatggatat tcgcggaatg gcggatatcc
    37921 gttacccgca agcagcctct catccaggtt ctgttcactg ttccgcaggc ggcgtcggac
    37981 gtaatatcgc gcataacctg gctctgcttg gtcgggatgt gcatttaata tcagctattg
    38041 gcaacgattt ttacggcgag acattactgg aagaaacgcg ccgcgcgggc gtgaacgttt
    38101 caaactgcat tcgtctgcat ggacacagca ctgcaactta tctcgctatc gctaataagc
    38161 aggaagaaac tatcctggca atcaatgaca cacatattct gcaacagcta acgcctcaac
    38221 tgctcaatac ttcacgggac ttgattcgcc atgctggtgt ggtgctggct gattgtaatc
    38281 tcacacctga agccctggag tgggtcttta ccattgctga tgaaattccg atgtttgtcg
    38341 ataccgtttc cgaatttaaa gcgaacaaag taaaaaactg gtacagccgg atccacactc
    38401 tgaaaccaac acaaaatgaa ctggaaattt tgtgggggca accgattaaa gacgacaacg
    38461 atcgcatcag agctgtaaat tcgctgcatc aacaaggtgt taaacgcatt tttgtttatc
    38521 tgaaagatga atctgtattt tgcagcgata aggatggaga acaatttttg ctgaccgcgc
    38581 cagcgcatac gacagtagac agttttggtg ctgacgatgg ttttatggcg ggcctggtat
    38641 atagctttct tgaaggatac agtttccgcg acagcgcccg ttttgcggta gcctgcgcgg
    38701 caatttcgcg cgccagcggc agcttaaaca accctaccct gtctgccgat aacgcgcttt
    38761 cattagtgcc aatggtgtaa caatgttgcc ggattcgacg cttgaccgca tcttatccgg
    38821 ctcataaatt gcgcactacg ctaaaccaat aaagaatcct gcaatagtag cactcatcag
    38881 gttagaaagt gtcgccgccg ccagcgcgcg taaaccaagt tgggcgattt ccggcgcacg
    38941 gtgtggcgca accgcagaaa acgcccccac caccaccccg atagaaccaa agttagcgaa
    39001 accgcacaac gcgaaagaaa taatcgccac ggttttagca tccagagtgc cacccgtttg
    39061 cagatatggc gagaaattga gataagcgac aaattcattg atcgccagct tctgcccaat
    39121 caaactcccg gcaagatttg catcactcca gtcaaccccc atcacccacg ccaatggggc
    39181 caacaggtaa cctaaaatcg actccagcga agcatgttca aaaccaaacc agccgccaac
    39241 accgccgata ataccgttaa tcaacgcaat tattgcaaca aatgccataa caactgtcgc
    39301 tacaccggcg gcgattttca gcccggtcat tgcccctgtc gcagcggctt caataatgct
    39361 ttttggcggt gtttcggtga aagagaggtt attaaaagaa acctgcgaag attccgtagc
    39421 cgggcttaac aggcgggcaa acaagatccc gccagggatc gccattaacg atgccgccag
    39481 caaatattcc acaggtacgc ccagtgcggc gtaaccaatc attgtcgaac cagcaatcga
    39541 ggccatgcca ctacaaatcg ctgtaaataa ttcattgcga ttcagacgat cgataaaggg
    39601 tttgacgatc gccggaattt cgttttgccc gaggaaaatg gtggtgaccg cgacgaatga
    39661 ctcgatcttg ctgatattta aagctttctg gaagataccg ccgagaatgc gaattaagat
    39721 ccccatcaca ccgatgtagt agagaatact caccagtgca gtgacgaaga taattgccgg
    39781 taataccctg aaaccaaaga taaatcctgc gccatcaaat aaggtatcca ttttcggtcc
    39841 gaccagagaa ccgaagataa atgcgctacc tgcgtcgctg tacgccatca ctttgtggac
    39901 gccaaaagcg actttttctg cgacccaacg ccctggcggt aaccaaagca taatcccgcc
    39961 aatcacgacc tgtaacacta acgccgcgcc aacggtacgc aggctgatct tcttcttgtt
    40021 tactgacagt aaaaacgcaa ttgccagcag taccaccatt cccagaacac ttctcatgac
    40081 atccatagtg attatctctt catgtcattt tgggcgacgg ctttctgccc atcagaaata
    40141 gccgggcctg catcttgcga tactgtcccg gcttgttatc aacacttaat gggtttttat
    40201 gtagccgcgt acgcactctt cgactaatcc ccagaaccag tcggtatcaa tagtgatgcc
    40261 gactttagta ttggcgggct tgccaagaac gcccagctcg tcgcagacgg tacgcccata
    40321 gcaagggcca ctgttgacgt ccacttcgac gtacatctcc tgggttttaa tgccatcagg
    40381 gttaatcaga taaccgatgc aggtggcgtc gtgcaccggg ccgccagcca ggccgtagtt
    40441 ttcgaactgc gttttgagag tgaagttcat gatgtcgctg aacatctctc cggcagggcc
    40501 gcctgccctt tccattcgtg caattacgtc cggggtgcaa acggtctggt tggtaagatc
    40561 gaggcccatc atcactaatg gaacgccgga ggtgaacacc acgcgtgcgg cttccgggtc
    40621 ggcaaagata ttgaactcgg cagatggggt gaagttgcct gtaccgtaag cgccgcccat
    40681 tagcacaatt tcacggattt tgggcaggat tgcgggttgc atacgcattg ccaccgcgat
    40741 atttgaaagc ggaccaaccg gcaccagagt gatatcgcca tcgcttgcca taagggtatc
    40801 aatgatgtat ttcaccgcgt gggtactttc tgcctggcgg gtcagcggct cgaataccgg
    40861 gccatccagt ccggtttcac cgtgaatatt atcggcaacg atttgttgac gcataatggg
    40921 ttgcggcatc cccgcataaa ccggaacatt aatctccagt ttctggcaaa cattcaggcc
    40981 attaattaat gttttatcaa gcgtctgatt acccgcaaca atggtgatac ctaataaatc
    41041 aatcgccgga tgtttcgccg ccatcattat agcaatagca tcatcatgac ccggatcaca
    41101 atccagaata atttttctct tttccattgt ttatttcctc tgtttccagt tgcgttattt
    41161 tttctacagc aacgaaattt cgatcgggcc gatgattgaa tcttaacaac agcgtacgaa
    41221 tgctaaatat gagaaatctc atagcggata aacatcgtga aagaaatcca caataatgat
    41281 cttaagcagc aattgatgag tgaatccgcg tttaaggatt gctttttaac ggatgtttca
    41341 gccgatacgc ggctgtttca ttttttagcg cgtgactaca ttgtgcagga agggcaacag
    41401 ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc tttacgccac gctagctaat
    41461 ggtcgcgtgt cgctgatcga tttctttgcc gccccctgtt ttattggcga gattgagtta
    41521 atcgacaaag accatgaacc gcgtgcggtg caggctattg aagagtgttg gtgccttgcg
    41581 ctccctatga aacattaccg cccgctgtta ttaaacgaca cgctattttt acgaaaactc
    41641 tgcgtcacct taagtcataa aaattatcgt aatattgttt ctttaactca gaatcaatca
    41701 tttccgttag tgaatcgcct ggcagcattt atattactct cgcaggaagg tgatctttat
    41761 cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt cttatcgaca tcttttatat
    41821 gttctcgcgc aattcattca cgacggttta ttaattaaaa gcaagaaagg gtatctaatt
    41881 aaaaacagaa agcagttgtc aggactggcg ctggagatgg acccggagaa taaattctcc
    41941 gggatgatgc agtaaaaatt attggcaata gcacgattgc cggatgcaac gcttaataag
    42001 gcgtacatcc ggcaatgaaa ctcaagctaa accaataaag aacccggcaa tggtcgcgct
    42061 catcaggttg gaaagcgtcg ccgccgccag cgcccgtaaa ccaagctggg cgatttccgg
    42121 cgcgcgtttt ggcgaaatag ccgaaaacgc tccaacgaca acaccgatag aaccaaagtt
    42181 tgcaaaccca cagagggcga acgagataat ggcaatggtc ttgacatcca gcgtgccgct
    42241 cgtttgcaga tagggcgaga aattgagata agcaacaaac tcattaatcg ccagtttctg
    42301 cccaatcaaa cttccggcaa gatttgcatc actccagtcc acccccataa tccatgccaa
    42361 cggggccagc acatagccga aaatgctttc cagagagaca ttggcaaaac caaaccagcc
    42421 gccaactcca ccaataatgc cgttgatcag cgcaatgatc gctacaaatg ccattaccac
    42481 cgtcgccaca ccggcggcga ttttcagccc ggtcattgca ccgttcgccg cagcttcaat
    42541 aatgcttttt ggcggtgttt cgctaaacga cagattttca aacgtaactt gcgaaggttc
    42601 cgtcgccgga ctaagaatac gtgcaaacag gataccgcca ggaatagcca tcagcgaagc
    42661 ggctaacaaa tagtcaatcg gcacccccat tccggcatag ccaatcatca tcgaacccgc
    42721 aatagacgcc atgccgctac aaatagccgt aaatagctcg ttgcgattca tgcgatcaat
    42781 aaaaggttta acgatcgccg ggatctcatt ttgcccgagg aaaatagtgg tgactgcgac
    42841 aaaagattcg attttgctga tgttgagggc tttctgaaaa atactgccaa gaatacgaat
    42901 cagcagcccc attacgccaa tgtagtacag caagctgata agcgcggtaa cgaaaataat
    42961 cgccgggagt acgcgaaagg cgaagataaa gcccgcaccg tcaaacagaa catccatttt
    43021 gggaccgacc agcgaaccaa aaataaaggc acttcctgcg tcactgtagg acatcacttt
    43081 atggacgcct aatgccgcct gctctactgc ccattttccc gggggaaaat agagcatgat
    43141 gccaccaatg gcaatttgca gcagcagcgc agcgccaacc gtgcgcaagc tgatgctctt
    43201 tttatttacc gacaacacaa aggcaattgc cagcaatacc accatccccg caacacttct
    43261 cattatatcc ataatgattt tcccttcatg ccagtaaacc cggcgtcagc gccaggtttt
    43321 ggtatgtttg atgagtacgg gcgacggctt tctgcccgcc agaaattaac ccacgagacg
    43381 ctgatattct ttggcaattt cgctcgccag aatggcgtta ttaaagacca gttgaatatt
    43441 ggatttcagg ctatcaccgc cggttagctc cgcaacacgc gccagcagga acggtgtact
    43501 ctctttccca ataacaccct gttcttcggc ttcagcaacg gcttgatcaa ttgctgcatt
    43561 gatggtttct tctggcatcg caaactgttc cgggatcggg ttcgctacca ccaggccgcc
    43621 gttcaaaccg gtttgccact tcactgccat cgcgcgagca atttcgcggg cactgtcaag
    43681 acgaatgctg acttcaaatg ggctggttcg gcagaaaaac gcaggcagcg cttttgtctg
    43741 ataaccaatt aacggtacac cgaaggtttc taaatattct gttgttaatc ctaaatcgag
    43801 aatagatttt gcccccgcac aaacaacagt gacattggta tttgccagtt cctgtaaatc
    43861 ggcagaaata tcgaaggtat gttccgctcc gcgatgcaca ccaccaatac ctccggttgc
    43921 aaaaacttta atcccagcca gtgctgcaat aatcatggtt gatgcaacgg tagtcgcgcc
    43981 atttttccct gcagcaacga caaaaggtaa atcacgacga ctaactttcg tcacgttatg
    44041 tccttcacgg cccagcaatt cgatttcttc tttacttaag cccactttca tcacaccgcc
    44101 aataatggcg atagtggctg gtactgcgcc ctgtttacga attgtttctt ctacttcaat
    44161 tgcggtttgg gcattttgtg ggaatggcat tccgtgagaa attatggtcg attccagcgc
    44221 aacaaccggt tttttatttt ttaaagcatc ctgtacttca gcggatattt gtaataattc
    44281 aggggacaga gttaattcag acattctgtg ttctccacta acgatctaac attggcaatt
    44341 gataattcag ggttgttggt atattcacag gcaagtgcca ttgacgaaca tccctgcgca
    44401 aatcgaacag aatcgataaa cggcataccg tctacccaac aggaggccag ccccgccatc
    44461 atggcgtcgc ctgccccagt aacattgatg acattcgttt taataggggc cgaccaccca
    44521 ctttcaccgt tgatatcact gtagtaaacg ccatcccctc ccatgctcag caccaagcgg
    44581 ttcaggccgt gttgatgaaa ccaggcggcg acctttgcga catcttcacg tccggaaagt
    44641 gcaattccgc tcaacgtttc ggcttcaaga cggttaggtt ttaacgtatg gattttgctc
    44701 agatgatcgc ggattttgac gcatttccat gccgataccg gatcaataaa tacaggcgtt
    44761 tccccgctat tttccagaac ccagaccagc acctcttcac taaggttgca atccgcgaca
    44821 ataacctttg caccgcgaat aaactcctga tgctgggcaa gaaattctgc agaaatacag
    44881 tcactaatac tcatatcatt tatcgcgaca agcatttctc ctgtattatc gagcagtgat
    44941 aaatagctgg atgtattctc acccggaaca attaagcatt tatcgacata aacaccggat
    45001 tgattagttt gcgccagtaa tgattgccca taaaaatcac cacctactgc acttaaaagc
    45061 caggcatttt tccccagtaa cgccagatta tgagcgatat tccttcccac gccacctggg
    45121 gtaaatttaa tttttccggg attcgaatcc gcataattca atggatgatg tgaataccca
    45181 gccacatcaa tattcgccga accaataatt actatgtagt ccttttcgcg catagcacgc
    45241 cccctggcaa taaattgcca acgaagaaat tgggtaaaat agccattaca gtcaataaga
    45301 ctattagagc acgtgttcag atttaaacac atgttcataa taagcatatt taacgtaatg
    45361 taaatcgatc agaaaaaacg aaatcggcca gatatgaagg cggtcacaaa gtttgtctat
    45421 ttatgcagca acaaaagaga aagttgtgat gtgtaacaca caatatggca ggcaagaaaa
    45481 agggcagaga tgtttctgcc ctccagattg ttgacaacgc gcgctttgtt catcctggat
    45541 gtggcgtaaa cgccttatcc gacctacaaa agcgcaaaaa attcaatata ttgcagggtt
    45601 catgtaggtc tgataagcat agcgcatcag gcaattttgc gtttgtcatc atcggggcag
    45661 agatgtctca tccccgataa tgaagataaa ttatgcgttt ttttctacta tttgtgtttc
    45721 cgggcgtttc aggaaggcat aggccaaacc cgccaccagc gtaccggcaa taattgctac
    45781 caggtaaccc aataccggcg taatagcgcc agggatcagc agaacaaaca gaccaccgtg
    45841 tggtgccatc agtttcgcac caatcgccat tgagattgcg ccagtcagcg ccccaccaac
    45901 gatacagcac ggtagcacac gcatcggatc acgagcagca aacggaattg caccttccga
    45961 aataaagcac agaccaagta ccagagcggc tttgccacct tcctgctgcg ctttgtcgaa
    46021 tttgcgacgc gccaccattg ttgccagacc cattgccagc ggtggcacca tacctgccgc
    46081 cataatcgcc gccatcgggc cataggtttg agtactcagc agacccacac cgaatgcgta
    46141 cgctgctttg tttaccggac cgcccatgtc agtacacatc atgccaccga ggatcgcccc
    46201 cagcagaacc gcattcgcag tccccatggt ctgcagccag tgagtcaacc catcgagaat
    46261 gccagcaacc ggtttaccga ttaggtagat catcgccaga ccgaccacca gactggaaat
    46321 tagcggaatg atcaggatcg gtttcagcgc ctccatactc tgtggcagtt tcaattgcgt
    46381 actgattaac ttcgcaatgt aaccagccag gaagccagca attataccgc caataaagcc
    46441 agaaccggtg ctgaccgcca gcataccgcc aatcagaccc ggtgtgaggc ccggacgatc
    46501 ggcaatggag aaggcaatat aacctgccag taccggcacc atcagcgcaa aggctgaacc
    46561 accaccaatc tgcatcagcg ccgctgccaa cgtacccggc tctttaaacg cttcgatacc
    46621 aaaagcaaaa gaaagcgcga tacacagacc acctgcaaca accatcggca gcatgtaaga
    46681 gacgcccgtc agcaagtgac ggtaagcgcc tgcactctct ttcttacctt cagtggtcgc
    46741 tgtttgagct ttgcccgctg gttcatacgg cgttgcttca gcaaccgctt tatccagttc
    46801 ctgcgcagtt ttcttcagcg ccagaccggt agaggtgcga tacatcggtt taccagcaaa
    46861 tttcgccaga tccacttcga tatctgccgc cacaatcacc agatccgctg ctgcgacttc
    46921 ttcaggggtg attgcattgc ccgcgccaac agaaccacgg gtttcaactt tcacccacca
    46981 gccacgtttt ttcgcttcgg tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc
    47041 agtcgggcaa gcagtcaccg caactacgcg tttcggaccg ctggcagcaa ccggtgctgt
    47101 cgcagcgacc ggcgcagtgt aaggtttcgc atggcctttg gcttcactca ggaacagctc
    47161 agggtgcgca actgcccggg aaatatcacc cagccagaca tttttaccgt tcagcgcgct
    47221 gtcattcggg atggaatcac cgagaacaat cgccatttct gcgtcgttcg gattgtcgat
    47281 gatttccagt tttgcttttc gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg
    47341 tgcctgaccg agattagcgt caataatcag cagcgttttc attatgcctc tcctgctgtc
    47401 agttaaaagg ttgtaagtcg acgcgcgcca tcattgcggc caactgcgga cgatcggtaa
    47461 tacccacatt gctttgactt accgccaggg ctgcaacagc tgtcgccaga cgcagtgtgt
    47521 gttcactgga ttcacgcatc agcaagccat aaatcaggcc accaaccata gaatcccctg
    47581 cgccaacggt gcttacgaca tcgactgacg gtggtttggc gatccattcg ccggaggcat
    47641 taacccaaag cgcgccttcg gcacccagtg aaataacaac atgcgcaata ccttgttcac
    47701 gtagcgcatg tgcagcttca atcacatctt tcatttcagg cagtttacgg cctgcccaga
    47761 tttccaactc gcggcggtta ggtttcacca gccacggtgc cgctttcaaa cctgctacta
    47821 acgcttcacg gctactatca aagataatgc aaggacactg actacgcagg cgagtcatcc
    47881 agtcggtgaa cgcttccggg ctgacgcctg acggtaagct tccgctgaca cagaccatat
    47941 cgaactgacc gagccagctc agagaatcag tcacaaagcg ttcccagtcg gcgagggtga
    48001 cttcaaaacc cgagaagttg aagtcggtca cttcgccgtc tttttccgtc agcttaacgt
    48061 taattcgggt gcgcccctgt acaacctgga aacggttggc aatgcccagc tcgctgaaca
    48121 gttgctgaaa accatcctga ttgtctttac ccaggaagcc gccaacggtg acatcaattc
    48181 ccaggtcttt taatactttg gccacgttga tgcctttacc cgccgcatgc agaccggtgg
    48241 ttttcaccag gttcacttcg ccgcgttcaa tttccgggca gaaaccaaca aggtcataag
    48301 ccggattaag ggtgatcgta gcaacacgtc tgctcattat gcgccctccc caagaccagc
    48361 agcgatagcg tcgccaatgg ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa
    48421 gcgtaggcga tgacctttct taacgccaag tgccacaact ttcatcagac tacgtccgtt
    48481 tgccggtttg ccggtgccat caaggtttgt cacggtaata tcactgttaa attgtttaat
    48541 ggtattgacc agcatggtac ctggacgagc atgcaggccg tgttcattgc gcaccacaaa
    48601 ctccgcgctt aacacgtcgt cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt
    48661 tgccgcatcc gctttcagca agcggtcagc tttattgtcg agcaataaat cagcgagacg
    48721 cttaagaacc gcgatgggct gatcgtcatt catcgccaca ctcaccagca tggctgccgt
    48781 ttcgccgtcc acatcaaaag catttgccgc acggcttacc gcaatcgcgc tacgcagatt
    48841 gccttcggcg ctatcgctca gccagatacc ctgtccgaga ttcagcggtt gttcattgat
    48901 ggctttggtg acgaaagtgg cgtcaactgc ccccacctct ttcagacgcg cagcgttcag
    48961 cgcctgaaga gtcagcagat cgctggcgac gatatccagc gtcagcattt cgttgtcgag
    49021 cttcagctgc tcactctgct tttcgcccat cagtaatgcg cgaagttctt ctgctgttgt
    49081 tgctgacttc agttgttcag caacggaatc atcgctcagt acgtgggtca gctggcgtag
    49141 caggcccaga tgttcatccg agctggcagc aataccgatt gccacgtacg ctacctgacc
    49201 gtcaccccag gtgacgcctt ccgggaactg aaatacctga acgccggttt tcagcacctg
    49261 atcgcgggtg tcggtagtgc cgtgtggaat agcaatacca ttgccgagga acgttgaggt
    49321 ttgctgttcg cgcgccagca tgccattgac gtagccttct gctacattac cggcctgcac
    49381 cagcgccgca gcgacctggc gaatcgcctc ttctttgtct ccggcctttt cgcccggatg
    49441 gatgtcctgt acggataact ggaacatagt tctcctctct tgctgaattg aaacgattca
    49501 gcctctatga gaaaaaaagc gccaacctgg cttagggtta aagacaagat cgcgctgaaa
    49561 cgtttcaaga aagcataata cttctgtttc agcacgcaag gaaagccgta aagttatgag
    49621 caaaagtttg atgtgctgca caattttttg tcatttttcc taattgctga tgggaaaatc
    49681 cgttatcagc aatttcattt cagcagcgtc agcagccaat cagcaacagt gaaatggcat
    49741 tttgattatt cgtggcgctt ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa
    49801 actccgcgtc ttcctcttcc agtgatcgac ctgcatgcat aactcccccg cagtctccag
    49861 cgcgaaatcg tttgacctga cctcgacggc gtttttaatc gttgcctttc tcaccggtat
    49921 tgcgggtgct ctgcaaaccc cgacactcag tattttcctt accgatgaag tacatgcccg
    49981 tccggcgatg gtgggattct tctttaccgg cagcgctgtc attgggattc tggtaagtca
    50041 gtttctcgct gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg
    50101 cctgttaggc gtgctggcct gcaccctttt tgcctggaat cgcaactact ttgttttgct
    50161 attcgttggc gtctttctta gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct
    50221 tgcccgtgaa catgccgaca aaaccggacg tgaggcggtg atgttcagct cttttttacg
    50281 cgctcaggtt tcactggcat gggtcattgg cccaccgctg gcttatgcgt tagcgatggg
    50341 tttcagcttt acggtaatgt atctgagcgc agcggtagcg tttattgttt gcggcgtgat
    50401 ggtgtggctg tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg gcacggttga
    50461 agcgccgcgc cgtaaccgtc gcgatacgct actgctgttt gtcatttgta cattgatgtg
    50521 gggctcgaac agcctgtaca tcatcaacat gccgctattt attatcaacg aactgcatct
    50581 tcccgagaaa ctggccgatg tgatgatggg gaccgccgcc gggctggaaa tcccgacgat
    50641 gttgattgcc ggatatttcg ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc
    50701 cgtgggtggc gtctgttttt acgcaggaat gctgatggcg cattcacctg tcattctgtt
    50761 gggcttgcag ctgctaaatg ctatttttat tggcattctg ggcggcatcg ggatgctcta
    50821 ttttcaggat ctgatgcccg gtcaggcggg ttcagccacc acgctctata ccaacacttc
    50881 gcgcgtgggc tggatcatcg caggatcagt ggcgggcatc gtcgccgaaa tctggaatta
    50941 tcacgctgtg ttctggtttg cgatggtgat gattatcgcc actctgtttt gcttactgcg
    51001 gattaaagat gtttaaggcg cggtcagcat ttccagatcg ataagccagg tcatggcctg
    51061 ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga ctttcggtcg
    51121 cagtggcgag gtgaaaattt tacagcgttg ctgttcatca agctgaatgc agggcgtatt
    51181 ggcgggcttg ccatctggca taccgggaat agggctggaa attgaagggg cagtgcaaca
    51241 cgccccgcaa cccggacggc attccatacg ttgttctctt aaggttaacc accggattga
    51301 tgagcgcgca cagtaccacc ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa
    51361 aggcccgtcg cgagtacttt gtcgcgatat ttttgacatt ttcgactaca ggaatttttc
    51421 gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg gcaaactgct
    51481 gctggttaag gatattgata ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa
    51541 aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca aaggtgatga
    51601 tatcgttgac accgtgacgc tgacccgccg ttacgttgac ttctcctatg tcgatggcaa
    51661 cgaatatgtc tttatggata aagaagacta taccccgtat accttcacca aagatcagat
    51721 tgaagaagag ttgctgttta tgccagaggg cggcatgccg gacatgcagg tgctgacctg
    51781 ggacggtcaa ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac
    51841 cgcaccaggc atcaaagggg catctgccag cgcccgtaac aaaccggcga cattgagcac
    51901 tggtctggtg attcaggtac cagaatactt aagcccgggc gaaaaaattc gtatccatat
    51961 cgaagaacgc cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag aaaagggata
    52021 gctcaggctg tccctttttt aatttattat accaatcttc tattttgcgc ttcctgaaca
    52081 ccttacgccc tcaattttca ctcgttgatt gatcgccctc acacttcatc gcattaacaa
    52141 tccagaccaa tttcaattgc tgtcatataa ctttacactg tcattgttaa tttatcgtta
    52201 ctaagacgtg actcctatga agacaattgc ctccgttacg ctcccgcatc atgtacacgc
    52261 tccacgctac gatcgccagc agttgcaatc acgtatcgtt cattttggct ttggcgcctt
    52321 tcaccgcgct catcaggcgt tactgaccga tcgtgtgctg aatgcccagg gcggcgactg
    52381 ggggatctgt gaaatcagct tgttcagcgg tgatcaactg atgagccagc tccgcgcaca
    52441 gaaccattta tataccgtgc tggagaaagg tgcggacggc aatcaggtga taattgtcgg
    52501 tgccgttcac gaatgcctta atgcaaaact ggattcctta gcggcaatta ttgagaaatt
    52561 ttgcgagcca caggtggcaa ttgtttccct gacgattacc gaaaaaggct attgtattga
    52621 cccggccacc ggtgcactcg acaccagtaa tccgcggatt attcacgatc tacaaacccc
    52681 tgaagaacct cactccgcac cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg
    52741 cggccttaca ccgtttaccg tgctctcctg cgacaatatt cccgacaatg gccatgtggt
    52801 gaaaaacgcg gtgctgggaa tggcagaaaa atgttcgcca gaactcgccg ggtggataaa
    52861 aaaacacgtc agttttccgg gaaccatggt cgaccgcatt gttccggctg caaccgacga
    52921 atcactggca gaaatcagcc agcatctggg ggtaaatgat ccctgcgcga ttagctgcga
    52981 accgtttatc cagtgggtgg ttgaagataa cttcatcgct gggcgtcctg cctgggaagt
    53041 cgcaggtgta caaatggtga atgatgtcct gccatgggaa gagatgaaac tgcgtatgct
    53101 taatggcagc cactcttttc tcgcttatct gggttacctc tcaggattcg tccatatcag
    53161 tgattgcatg caggatcgcg tatttcgcca tgctgccaga acattaatgc tggatgagca
    53221 agcgccgaca ctgcaaatta aagatgtcga tttaacacaa tatgcggata agttaattgc
    53281 acgttttgct aatccgtcgc tgaaacataa gacctggcaa atcgcgatgg atggcagcca
    53341 gaaattaccg caacgtatgc tggcaggtat tcgcatacat ctggggcgtg aaacggactg
    53401 gtcgttgctg gcattaggcg ttgctggctg gatgcgttac gtcagcggcg ttgatgatgc
    53461 cggaaatgcc attgatgttc gcgatccgct tagcgataaa attcgcgaac ttgttgcggg
    53521 cagcagcagt gaacaacgcg taaccgccct gctttccctg cgtgaagttt tcggtgatga
    53581 tctgccagat aacccgcatt ttgtgcaggc catcgaacaa gcctggcaac aaatcgtaca
    53641 attcggcgca catcaggcgc tattaaacac cctcaaaatt taacgatttc tgcggttaaa
    53701 gcggatgaag ctcaccttcg tccgctctcc ccttctcttt tctgcctttt ttagccagga
    53761 ttaacgctca gttaacttac cagagtattg cggagccgtt gtgaccagga ccaacctcat
    53821 caccggtttt ctcggcagcg ggaaaaccac gtcgattctt catctgttag cccataaaga
    53881 tcccaacgaa aaatgggcgg tactggttaa tgaatttggg gaagtcggaa ttgatggtgc
    53941 tttgctcgcc gatagcggcg cattgctgaa agagatcccc ggcggctgca tgtgctgcgt
    54001 taatggttta cccatgcagg tagggttgaa taccttactg cgtcagggaa aaccagaccg
    54061 cttgttgata gagccgaccg ggctgggcca tccgaaacag atcctcgatc tgttaaccgc
    54121 accagtctat gaactgtgga tagatctgcg cgccaccttg tgcattctcg atccacgcct
    54181 gctgctggac gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg ctgccgcaga
    54241 catcattgtc gccaataaat ccgaccgtgc gacgcccgaa agtgagcaag cgctacagcg
    54301 ttggtggcag caaaatggtg gcgatcgaca attaattcac agtgagcatg ggaaagttga
    54361 cggtcatctt ctggatttgc cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca
    54421 ttctcatcag catgtcgtga aaaaagggtt agcagcgtta agcctgccag agcatcaacg
    54481 ctggcgtcgc agtctgaaca gcgggcaagg atatcaggcc tgcggctgga tattcgacgc
    54541 tgatacggta ttcgacacca ttggcattct ggaatgggcg cgacttgcac cggtggaacg
    54601 cgtcaaaggc gtgctgcgta ttcccgaagg gctggtacga atcaaccgtc agggcgatga
    54661 cctgcacatt gaaacgcaaa acgttgcgcc accggacagc cgtattgagc tgatttccag
    54721 cagcgaagct gactggaatg ctttgcagag cgcgctgttg aagcttcgtt tagcgactac
    54781 cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag aaaatatgat taaaaatttg
    54841 ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt ttctttcctg gtatattccc
    54901 gttaatcatg gattctggtt gccgattgat gcggatattt tttatttctt taatcagaaa
    54961 ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga ccaacaatcg cgctttcgac
    55021 ggttgttctc tgctggcgat gggaatgttg atgctgagtt tctggctgaa agaaaacgcc
    55081 cctggcagac gacgtatcgt gattatgggt ctggtcatgc tattaactgc agtggtatta
    55141 aaccagctgg gtcaggcatt aattcctgta aaacgggcca gcccaacatt gacttttacc
    55201 gatattaacc gcgtcagcga actgctctct gttcccacga aagatgcctc acgagatagc
    55261 tttcccggcg atcacggcat gatgctgctt attttttcgg cattcatgtg gcgttatttc
    55321 ggcaaagttg caggccttat cgcccttatt atttttgtgg tttttgcatt tcccagagta
    55381 atgattggcg cacactggtt tactgacatc attgtcggtt cgatgaccgt gatattgatc
    55441 ggtttgccct gggtgttgct gacgccatta agtgatcgat taatcacctt ttttgacaaa
    55501 tcactaccgg gaaaaaacaa acatttccaa aacaaataac tcacagtaat taacatcatc
    55561 aggcttattt ttatagtgag gataatcctg atgatgcgca ccgtgctttc atccatcgcg
    55621 cgcaaaaatc attctctaag taaatgaatg gattgcatgc gtttcactca atcgtacttt
    55681 aattgaccaa tcccgcttat taactttctg tatcactttt tcttataaaa aatcatgtaa
    55741 aaccgctcgc caagaccgca ccaatcgggt aatctagaac tcgttttgcc tcggcggtag
    55801 attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt aaggaattgt
    55861 ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat ttttttataa cgatatttgt
    55921 cgttaaggac ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat tttgagatat
    55981 atcttgcgcg ggatccccgc gattgcagta gcggttctgc tttctgcatg tagtgcaaat
    56041 aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg
    56101 caagcttctc aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt
    56161 atggatcagt atgctgactg gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa
    56221 ggtatcgatt gttctggttt tgtacaacgt acattccgtg agcaatttgg cttagaactt
    56281 ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt
    56341 acgggtgatt tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg tatttatatc
    56401 ggcaacaatc agtttgtcca tgcttccacc agcagtggtg ttattatttc cagcatgaat
    56461 gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg cagctaataa
    56521 accgtttgga tgcaatccct tggctatcct gacgagttaa ctgaaagcac tgcttaggca
    56581 gtgctttttt gttttcattc atcagagaaa atgatgtttc cgcgtcttga tccaggctat
    56641 agtccggtca ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg
    56701 ttcatacgcg ctcccaattc tggacgtaag ctcctgctta cctgcattgt agcaggcgtg
    56761 atgattgcga tactggttag ctgccttcag tttttagtgg cctggcataa gcacgaagtc
    56821 aaatacgaca cactgattat cgacgtacaa aagtatctcg atacctattt tgccgacctg
    56881 aaatccacta ctgaccggct tcagccgctg accttagata cctgccagca ggctaacccc
    56941 gaactgaccg ctcgcgcggc gtttagcatg aatgtccgta cgtttgtgct ggtgaaagat
    57001 aaaaaaacat tctgttcatc tgcgactggt gagatggaca ttccactcaa tgaattgatt
    57061 ccggcgctca acattaataa aaacgtcgat atggcgatct tacccggcac gccgatggtg
    57121 ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa tagcggtgtg
    57181 tttgccgctc tgaatctcaa cctgacgcct tctctctttt atagttcacg gcaggaagat
    57241 tacgatggcg tcgccctcat tattggcaat actgcgctat ctaccttttc ttcacgtttg
    57301 atgaacgtta atgaattaac cgacatgccg gtccgtgaaa ctaaaattgc gggcattcct
    57361 ctgaccgttc ggctttatgc ggatgactgg acatggaacg atgtgtggta cgcattttta
    57421 ctgggcggca tgagtggaac tgtcgttggc ctgctctgct attacctgat gagcgtacgt
    57481 atgcgccccg gcagagaaat catgaccgcc atcaagcgcg aacaatttta cgtggcgtat
    57541 caaccggtgg tggatacaca agctttgcga gtaacgggcc tggaagtact gctacgctgg
    57601 cggcatcctg tcgcgggaga aattcccccg gatgccttca ttaactttgc cgaatcgcaa
    57661 aagatgattg tgccgctgac tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa
    57721 ttagaaaaag tgctgccagt aggcgtcaaa tttggtatta acattgcgcc ggaccatctg
    57781 cacagcgaaa gctttaaagc agatatccag aaactgctca cttccctgcc cgcacaccat
    57841 ttccagattg tgctggaaat taccgagcgc gatatgctga aagagcaaga agccacacaa
    57901 ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta ttgatgactt cggcaccggg
    57961 cacagcgcgc ttatctatct tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga
    58021 tttatcaacg ccatcggtac ggaaacgatc acttcacccg tacttgacgc ggtgctgacg
    58081 ctggcgaaac gcctcaatat gctgacggtt gctgaagggg tcgaaacgcc ggaacaggcg
    58141 cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct actggattag tcgcccttta
    58201 ccgctggacg attttgtccg ctggctgaag aaaccgtata cgccgcagtg gtaaggtgtg
    58261 cttacgtccc ttattattca tagtgaaagc atgccggatt gcggctaatg atgagtaaaa
    58321 ggaaatccat tgcagatgat tgtgcgcata ctgctgctgt ttatcgctct gttcaccttt
    58381 ggtgtgcagg cgcaggctat caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg
    58441 tacgcgttta atttcaacca ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag
    58501 ataacgttgt cagccctcgg caccttcgat aatttcaacc gctatgcact gcgcggcaac
    58561 ccgggcgcac gcaccgagca gctgtacgac acgctattta cgacttccga tgacgaacca
    58621 ggcagttatt acccgctgat tgctgaaagc gcacgctatg ctgacgatta ttcctgggtg
    58681 gaggtcgcta ttaatccgcg cgcccgtttt catgatggtt cgcccattac tgcccgcgat
    58741 gtagagttta cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg tctggtctac
    58801 aaaggcacca ccgtcaaagc cattgcaccg ttaaccgtgc gcattgagtt agctaaaccc
    58861 agcaaagaag atatgctgag tctgttttcg ctgccgatat ttccagaaaa gtactggaag
    58921 gatcacaaac ttagcgaccc gctcgccacg cctccgcttg ccagtggtcc gtaccgcgtt
    58981 acgtcctgga aaatggggca aaatattgtc tattcccgcg tgaaagatta ctgggcagca
    59041 aacttaccgg taaaccgtgg acgctggaat ttcgacacca ttcgctacga ttattacctc
    59101 gatgataatg tcgcctttga agcgtttaaa gcaggtgcct ttgatttgcg tatggaaaac
    59161 gacgctaaaa actgggccac gcgttatacc ggtaaaaatt tcgataaaaa atacatcatc
    59221 aaagatgagc aaaagaacga atcagcccag gatacgcgct ggctggcgtt taatatccaa
    59281 cgtccggtat tcagcgatcg ccgggtccgg gaagcaatca ctctcgcctt tgactttgaa
    59341 tggatgaaca aggcgttgtt ttacaacgcc tggagtcgca cgaacagtta ttttcagaat
    59401 accgaatacg cggccagaaa ttaccccgac gccgcggagc tggtgcttct ggcaccaatg
    59461 aaaaaagatc taccgccaga agtcttcaca caaatctacc agccgccggt atctaaaggc
    59521 gatggctacg atcgtgacaa cctgttaaaa gccgacaaac ttctcaacga aacgggctgg
    59581 gtgctgaagg gtcagcaacg cgttaatgcc acaacgggtc agccgctcag ctttgaatta
    59641 ttgcttcccg caagcagcaa tagtcagtgg gtattgccgt tccagcacag cctgcaacgg
    59701 ctgggtatca atatggacat tcgcaaggtg gataactcgc aaatcactaa ccgcatgcgc
    59761 agtcgcgact atgacatgat gccgcgccta tggcgggcga tgccgtggcc cagttccgat
    59821 ttacagattt cctggtcatc ggaatatatc aattccactt ataatgcccc cggcgtgcaa
    59881 agcccggtta tcgactcgct gatcaaccaa attattgccg cgcagggaaa taaagaaaaa
    59941 ttactgccgt tggggcgagc actggatcgc gtattaacgt ggaattatta catgctgcca
    60001 atgtggtaca tggcggaaga ccgtctcgcc tggtgggata aattctccca acccgctgta
    60061 cgccctgttt acagcctggg tatcgatacc tggtggtatg acgttaataa agcggccaaa
    60121 ctgccgtcag ccagacaaca gggagagtag atgggcgctt acctgattcg ccgtctgttg
    60181 ctggtgatcc caacgttatg ggctattatc actatcaact ttttcatcgt gcaaattgcg
    60241 cctggcggtc cggtcgatca ggccatcgcc gccattgagt ttggtaatgc cggagtatta
    60301 cccggcgcag gcggtgaagg tgttcgtgcc agccatgcgc aaacgggtgt cggcaatatc
    60361 agcgacagta attaccgtgg cggacgcgga ttagacccag aagtgatcgc tgagatcact
    60421 catcgctacg gtttcgataa gccgatccac gaacgttact tcaaaatgct ctgggactac
    60481 atccgctttg attttggtga tagcctgttt cgcagcgcct cggtgctgac gctgattaaa
    60541 gacagtctgc cggtttccat caccctcgga ttgtggagca cgctgattat ctatctggtg
    60601 tcgattccgt taggcattcg caaagctgtt tataatggga gtcgctttga cgtctggagt
    60661 agcgcattta tcatcatcgg ctacgccatt ccggcctttt tgtttgccat cctgctgatt
    60721 gtcttcttcg cgggcggcag ctatttcgac ctgttccctc tacgcggcct ggtttccgct
    60781 aactttgatt cgctgccgtg gtatcagaaa atcaccgatt atctgtggca tatcacgcta
    60841 ccggtgctgg cgacagtgat tggtggcttt gcggcgctga ccatgctgac caaaaactca
    60901 ttccttgatg aagtgcgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg ggtaagtgag
    60961 aaaaacattc tctggaaaca tgtgttccgc aacgccatgc tgctggtgat tgccggtttt
    61021 ccggcgacgt ttatcagcat gttttttacc ggctcgctgc tgattgaggt gatgttttca
    61081 ctcaatggtc ttggcttact gggctacgaa gcgaccgtct cgcgcgatta tcctgtaatg
    61141 tttggtacct tgtatatttt caccctgatt ggcctgctgc tgaatattgt cagtgatatc
    61201 agctatacgc tggttgatcc gcgtatagat tttgagggac gttaatgtcg cgactcagcc
    61261 ccgtcaatca ggcccgttgg gcgcgttttc gtcataaccg tcgcggctac tggtcgttat
    61321 ggattttcct cgttttgttt ggtttgagtt tgtgttctga acttatcgct aacgataaac
    61381 cgttgctggt gcgttatgac ggcagttggt atttcccgtt gttgaaaaac tacagcgaaa
    61441 gcgattttgg cggcccgctg gcaagtcagg ctgattatca ggacccgtgg ctgaaacaac
    61501 ggctggaaaa taacggctgg gtactgtggg caccgattcg ctttggtgct accagtatca
    61561 actttgctac cgataagccc ttcccttctc caccctcccg gcaaaactgg ctgggaacgg
    61621 atgccaacgg cggcgatgtg ctggcacgca ttctctatgg cacgcggatc tcggttctgt
    61681 ttggcctgat gctgactctc tgttccagcg tgatgggcgt gctggcgggg gcgctacaag
    61741 gctattacgg cggtaaagtc gatctctggg gacaacgctt tattgaagta tggtcgggga
    61801 tgccgacgct gtttttgatt attttacttt ccagcgtcgt acagcctaac ttctggtggc
    61861 tgctggcaat tactgtcttg tttggctgga tgagtctggt cggcgtggtg cgggcggagt
    61921 ttttacgtac tcgtaatttc gactacattc gtgcggcaca ggcgcttggc gtcagcgatc
    61981 gcagtatcat cctgcgtcat atgttgccta atgccatggt cgcgaccctc acctttttac
    62041 cgtttatttt atgtagttcg ataaccaccc tgacctcgct cgatttcctc ggcttcggtc
    62101 taccgctcgg ttcaccgtca ctcggtgaac tgctgttaca agggaaaaat aaccttcagg
    62161 ccccgtggct tgggatcacc gccttcttgt cggtggcgat attattgtct ttgctgatct
    62221 ttattggtga agccgtccgc gacgcatttg atcctaataa ggcggtgtag catgacgcaa
    62281 actctgttag cgattgaaaa tttgtcggtg ggttttcgcc atcagcaaac cgtacgtaca
    62341 gtagtcaatg atgtttcact acagattgag gctggcgaaa cgctggcgct ggtgggtgag
    62401 tcaggttcag gcaaaagcgt taccgcgctg tcaattttac gcctgctccc ttccccgccg
    62461 gttgaatatc tctccggcga tattcgtttt catggcgaat cgctgcttca tgccagcgat
    62521 caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcagga accgatggtg
    62581 tcgttaaatc cattgcatac cctggaaaaa cagctttatg aagtgctttc actccaccgc
    62641 gggatgcgtc gggaagcggc tcgtggcgaa attcttaact gccttgatcg cgtcggtatc
    62701 cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg cgaacgccag
    62761 cgggtgatga ttgcgatggc gctgttaacg cgaccggaat tattaattgc cgatgaaccg
    62821 accaccgcac tggacgtctc tgtccaggcg cagattttac agctgttgcg cgaactgcaa
    62881 ggcgagctga atatgggcat gctgtttatt actcataacc tcagcattgt cagaaaactg
    62941 gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agcaaaataa cgccgctacg
    63001 ctatttgcct cacccactca tccttacaca caaaagctac tcaacagtga accgtcaggc
    63061 gatccggtac cgttgccaga acctgcctca acgttgctgg atgttgaaca gcttcaggtt
    63121 gccttcccca ttcgcaaagg gattttgaag cgcattgtgg atcataatgt ggtggtgaaa
    63181 aacatcagtt ttacgctacg ggcgggtgaa acactgggtt tagtgggcga gtctggttcc
    63241 gggaaaagca cgacgggact ggcgctgctg cgactgatta cctctcaggg cagcatcgtc
    63301 tttgacggtc agccactgca agatttaaat cgccgccagc tgttacctac tcgtcatcgt
    63361 attcaggtgg tatttcagga tccaaactcc tcgctcaacc cacgactcaa cgttttgcag
    63421 attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca acgcgaacaa
    63481 caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca ccgttatccg
    63541 gcggagttct ctggtggtca gcgacaacgt attgcgattg ccagggcatt aattcttaag
    63601 ccctcactga tcatacttga tgaaccgaca tcatcactcg acaaaacggt acaggcgcaa
    63661 atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt gtttatcagc
    63721 cacgatttgc acgttgtccg cgcgttatgt catcaggtta tcgtactgcg acaaggggaa
    63781 gtagtggaac aaggaccgtg cgcgcgcgtg tttgccgcac cgcagcagga gtatacgcgt
    63841 cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg gttcggcaat
    63901 tgccacacca aaatttttca atcggcatgt tgcagcaaac tcgtcctggc gatttacaaa
    63961 caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt cgcttaacgc
    64021 ctggcttagt ttatacatca ccgaccatgc attatcacca tccgggctaa gcaatttaag
    64081 cgccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa ccttcgaacc
    64141 tgcgaaacca gcagaccagc gaaagttctg cggtagacga tggacgattg agagttgtaa
    64201 tgaagtcact tattttcccc ggaagcacat ttacttcaca atttatttac atcaatttta
    64261 acacatcatc aacaatccgt ctttatacag atcgtaaaga ttgatgcctc gcctgcatgg
    64321 cctctatggc tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg gggctatatg
    64381 tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact ttttacataa
    64441 attgatttta cataaaataa acatatatcg ggggaatgat agatttgtgg ttgacatgca
    64501 tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag atcaccgttt tttcggtcga
    64561 ctggcgtaca gacagaagag aatggagctg gttgcgcaga atgcaattga ccaaatcatc
    64621 ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc caacaattgc ccctatgcca
    64681 aaacggaagg ttcctgccag cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga
    64741 atgaccgcca tcgcattgga tgacaccata gacacgcagc ccacaaacgc cgcaacgcca
    64801 accaccagcg accagaatcc cagccccagc agcgcactga tgaccatcca cgctgccata
    64861 ataaattgta tccacaaccc cgagcggaac atatttaacg cgccaatgcg gcggacgaag
    64921 cggctgttaa agatggtcat cacgaacaga aaaacaatgt ttagcgcaaa gtaataacca
    64981 aagttttccg gtgcaacgtg gttgatttca atgtagacaa acggtccggc gctcaggaac
    65041 gagaacattc cggcaaagct gaaaccgctg gccagcatat aactcaggac acgcttatga
    65101 cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg gcgaggatcc
    65161 agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc ggtgccatca
    65221 gcggtgcaat ggtcgtcacc agcatgacaa acgacatcat ccgcgagaac tcttctttcg
    65281 ggtaaatatc acgcatcaaa gcgttgataa ccacgctggc cgcagccgca gccagcccgt
    65341 ggaagaaacg catcacaatc agctgatcga tggtttgtgc caacgcacac gccaccgcgg
    65401 cggcggcaaa caccagcgta ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca
    65461 tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactc agggtcatct
    65521 gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg ggcagataca
    65581 tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg
    65641 acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt tagacgacgc
    65701 tggcaatttc ttcttcagtt aacggacgat attcaccggg ggctaaatca gcatccagcg
    65761 taatgccgcc aatacgttca cgatgcagct caaccacatg gttacccacg gcggcgaaca
    65821 tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa
    65881 tcacttccag caccgccggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag
    65941 caaattgttc tgccgtatcg tcagctacgg gtgataccag cgtcaccaga taggtcttct
    66001 cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg accatcatct gtcattagta
    66061 ccagaccggt ggtatcgata tccagccgcc ctgcggcgtg cagtttccac gctaccggtt
    66121 catctagaaa atagagcacc gttgggtgat cagggtcgtc cgtggagcaa acataaccct
    66181 gaggcttatt gagcatgaag taacgtggac cgtgttgctg cgccagcggg ttgccatcgt
    66241 aagcgacatc atgttcagga agcagtttga acgctgcatt acggacgatt tcgccatcga
    66301 cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct
    66361 gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaaac ggaggtcggg
    66421 taatgacccg aatatctgaa ctgttgtctg cccagtatag cggtctaata acatccctca
    66481 agggaaaaag atccgtggca tactattagc agaataatct acctacgcga gaccatgatt
    66541 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    66601 cataaaaccc ctgccgttat cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg
    66661 gaactggcac ggctggctcg tggtcgcgta ctggtgctgg cacacgttaa agaactggtg
    66721 gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgccggg
    66781 ctgaagcgta aagagagcca cggtaaagtg gtatttggca gtgtgcagtc ggtcgcccgt
    66841 aatattgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt
    66901 ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgacaaa agtgaatccc
    66961 cacttacgcc tgctggggct gactgccacg ccttttcgat tgggcaaagg ttggatctac
    67021 cagttccatt atcacggcat ggtacgcagc gatgagaaag cccttttccg tgactgcatt
    67081 tatgagctgc ccctgcgtta tatgattaaa cacagctatc tgacgccgcc agaacgactg
    67141 gatatgccag tagtgcaata tgatttcagc cgcttgcagg cacagagcaa cgggctgttc
    67201 agcgaagccg atctcaaccg tgagctgaaa aaacagcaac gtattacccc gcacatcatc
    67261 agccagatta tggagttcgc cgaaaagcgt aaaggagtga tgattttcgc cgcgacggtc
    67321 gaacacgcaa aagagattgt gggattactg cctgccgaag atgcagcact gattactggc
    67381 gacacccccg gcgctgagcg cgatgtgtta attgaaaatt ttaaagccca gcgttttcgc
    67441 tatctggtca acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    67501 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    67561 cgtctcgctc cgggcaagac tgattgttta attcttgatt atgcgggtaa tcctcacgat
    67621 ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt
    67681 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    67741 ttgattgaac actttggtcg ccgctgtcag ggatggtttg aagatgacga cggtcatcgc
    67801 gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc agaaaatgat
    67861 attgccgctc gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta
    67921 aaagcggcgc tacgactgaa agatgcgctg gtattacgct gtagcggcat gtctttgcag
    67981 catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggtgcg
    68041 gatgtgagtg agcgtttccg tctgcaaaca cctgcccagc gtaccgcctt cgagcagctt
    68101 tttatccgcc cgcatacgcg cacaccgggc atcccgctgc gctggatcac cgccgccgat
    68161 atcctcgccc agcaagcctt attgcgaccc ccggattttg tcgtcgcccg catgaaaggc
    68221 cagtactggc aggtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac
    68281 gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta tagaatctca
    68341 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    68401 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    68461 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    68521 agaagcgccg ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc
    68581 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    68641 tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc
    68701 ttaattgctg aataagttgt aaaaaacccc gctcccgcgg ggttttttgt atctgcagat
    68761 tatgcctgat tacggtattg ctattttttg caggccagat aaggcgttca cgccgcatct
    68821 ggcattcaga acacaaaacc gattaattgc cgccagacgt ccggcgctgc aattggtcgc
    68881 gcaaattcgg cggcgtgcct ttaatggtca gggtatcggt cgccggctcc cagaagatgc
    68941 gttcacctaa cagcatcgca tcaaagttaa tagtcaagcc accgccgcta ccggcaaact
    69001 tcgtcaactg acgcagtgtg ctccggtctg ccgggaagct ctcttccagt tcgtagcctt
    69061 tttcagccgc aaactccgtg aagctcactt cgcttacgcc tgccagctct ttcgacagcg
    69121 attccagttc gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct
    69181 ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg
    69241 cttgcaacag cccacggttt tgtgctttcg tgttcaatcc ttcgctggcc ccgaggaaat
    69301 ccataaagaa atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg
    69361 tggactctgg attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat
    69421 tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacacgc atactgctca
    69481 ggttgctcag aactgccacc agcagatact ccaccgccag ataacggtag tggcaaaaca
    69541 gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgaccagttg
    69601 ccgcgcggct aaatgccaga aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg
    69661 ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat
    69721 gcagttctgc caccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca
    69781 ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat
    69841 ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc
    69901 gacgcaataa aagatggcgg tcattttaca gaaggcagcg taacgagaat gatttaacgc
    69961 ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact
    70021 gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg
    70081 cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt
    70141 taggcaacat ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa
    70201 ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag
    70261 ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag
    70321 atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc
    70381 agccgttacc tgtttatcgc cgactggccg acaacgcttg ctggtcgcat ttattcctac
    70441 gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc
    70501 ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca
    70561 acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat
    70621 cttaatccca tcgtctggca gttggttatc aacccagacg aaaatgagat ggcgcgcgac
    70681 tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg
    70741 tggagctggc aaaagctgcg cagcctgacg cgtcgtcgac gcttcgcgcg cccgctggcc
    70801 gcattcttat ttatcgcctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac
    70861 ttctatcgcc cgatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg
    70921 cgacgttttc ttgagaagca tggtctgctt gatgcgcagg agtatcaacg ccgtcttatt
    70981 gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc
    71041 gatatgggca ccgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc
    71101 ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc
    71161 catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta tggtatctcg
    71221 ccgagctata tggacggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt
    71281 aatcagcaag gctatcagct ggggttattc tcatcagatg gctttaccag cccgctgtat
    71341 cgccaggcat tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag
    71401 accgccacgc agtggatcaa ctggctgggc cactacgcac aagaagataa ccgctggttc
    71461 tcgtgggttt ctttcaatgg cactaacatt gacgacagca atcagcaggc atttgcacgg
    71521 aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg
    71581 cgtgattctg gcaaactgga caatacggtt gtgattatca ctgccggtcg gggtattccg
    71641 ctgagcgaag acgaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg
    71701 attcactggc caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg
    71761 atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa
    71821 ggtcaggatt tgttcaaccc tcaacgccgt cattactggg ttactgcagc ggataacgat
    71881 acgctggcaa ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc
    71941 acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg
    72001 caagtgctga cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg
    72061 aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt agcagccacg agtcggcacg
    72121 tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc
    72181 gtgccgacca aaaatcccaa gaaaaaacca acctttacgg ttggtttttt tatatctgca
    72241 attaatttga taaacagacc gtgacacatc acagcctgtt tattttctgt tatcagaacg
    72301 tccagaccac tcccgcctga gtattccacg gcgattctac ccctgcgcca ttgccatagc
    72361 tgactgacaa atgaccgctt aacgtcgggg taaacgatga ccttatccca gcctgataaa
    72421 cgccacgggt tccagccaca tcattattaa atttgccatc gtcattaact ttcacatggt
    72481 tagaatcggc gtattcctga cgcacggcgg ctttcagcca gggttccaac gtcgtaccgt
    72541 tttgcaggtc catgtgatag cttaccgccg ttcccgcttc agcgcgtaat atccgggtat
    72601 ttcccacatc cgcgcgcatg ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc
    72661 cggtaaaggc gagatagggt ctaacactcc acaatccgtc aacccaacgg aacccgctct
    72721 caacatgagc acccgcgccg ttactattgt aatcgccaaa cgctgttgcc ccattactca
    72781 tcttgccatg gatggtgttg gcaaaacggt caactttcac caccccatca acataggcac
    72841 cgttctgatg ctcccaaccg gcataagccc ccagggtgta gctatcaata ttgcctttgc
    72901 cgccgcgatc aaaaccaata tcagaatgag agtaatcaaa gaacaagccg cgaattgtgc
    72961 tgctttcttc acgggagaaa cggctatcga taccgagcgt caggcccgtc aatgtttgct
    73021 caaagccagc tcccgcatca gtggtcacgt tgttgcgggt gttaattgcc gaactccaca
    73081 tcgccgtatc gtaattaacg ccttttacgc taccaagacg ctcacgcacg gtgtccagtt
    73141 ctgcatcaaa taccagcggt tgtgctgctg ccatgttcag cacatcagtg gttgaagggg
    73201 taatttgcgc gcgattctct gcgaggctcc agctatggtt gccattatcc agcaaggtat
    73261 attcatacgt accgatatca acaacgcctc cggcattgcc caacgtgaat gcagcatcac
    73321 cgccgcccgt cgttaccagt gtaaggctat ctcctgctgc cgggctggcc cccgtgtccg
    73381 tcacgaatat tttgaaatca cctgttgcct gaccgatgac gttgagctga tcgctctgat
    73441 gattagccat atcggtacgc atataaaatt gccgtttccc gacagggtat tggtggtcaa
    73501 cgtaatgaaa ttttccgcac ttgccgtcac agatgaacga gtcactggct cagccatcat
    73561 aatcacattt gcgccggaca tcgcaaggtt ggctaagttg aggcgagtgt tttggttcgt
    73621 cgtagtgata tcggcaatga tggtccatgc gctattttcg agcgaaacgt ccgcttggct
    73681 ggtatccacg gcaccatgcg ttctaaaaga agcgccctca gaaaccacta ccttacccac
    73741 caacatagcg ggtgcataat ttattttcgg cttcatgacc tcaagaatgc catcattccc
    73801 tctgactgac acgttaacca ttgtgccagc ccagacatta gcgcggccat tgttgataac
    73861 catattttca gagatggctt tagaggaata cgtgatactt ccattctgat ctgatcgacc
    73921 gagatcatat acagcatttg cctgcacttt cgtaccagta tcctttccca gcgattgcat
    73981 agtggcatgc tcatcaagga tagtgtcaat ggcctgagtg tcctccataa caatgagccc
    74041 ggagccatca tccagttcat aattttttga cacaccatcc tttatactaa attggccttt
    74101 actgtttgta ccactcactt ccagcgcatt cgttgagaca attaatttcc cgcctgcttt
    74161 tttatcaacg ccagttgcag tcccgccatc cataacctcc agtaatcctc ccttttcgat
    74221 agcggcaaag acggcaatcc catctttttc aacaattaag cgcccgcctt taatcgtggg
    74281 atcattcgcc gcg
//
LOCUS       DAJVYM010000004        60958 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVYM010000004 DAJVYM010000000
VERSION     DAJVYM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.004"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..468
                     /locus_tag="OQS60_000236"
     CDS             <1..468
                     /locus_tag="OQS60_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2386102.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="OQS60_000237"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="OQS60_000237"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS2386103.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="OQS60_000238"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="OQS60_000238"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS2386104.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OQS60_000239"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OQS60_000239"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS2386105.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="OQS60_000240"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="OQS60_000240"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS2386106.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="OQS60_000241"
     CDS             complement(4084..5313)
                     /locus_tag="OQS60_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS2386107.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="OQS60_000242"
     CDS             complement(5377..5817)
                     /locus_tag="OQS60_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS2386108.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="OQS60_000243"
     CDS             complement(5922..6692)
                     /locus_tag="OQS60_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2386109.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="OQS60_000244"
     CDS             complement(6689..7615)
                     /locus_tag="OQS60_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2386110.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="OQS60_000245"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="OQS60_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS2386111.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OQS60_000246"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OQS60_000246"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS2386112.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="OQS60_000247"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="OQS60_000247"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS2386113.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OQS60_000248"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OQS60_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS2386114.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="OQS60_000249"
     CDS             13322..13669
                     /locus_tag="OQS60_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS2386115.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="OQS60_000250"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="OQS60_000250"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS2386116.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="OQS60_000251"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="OQS60_000251"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS2386117.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="OQS60_000252"
     CDS             complement(15489..15851)
                     /locus_tag="OQS60_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS2386118.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OQS60_000253"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OQS60_000253"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS2386119.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="OQS60_000254"
     CDS             18978..20735
                     /locus_tag="OQS60_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS2386120.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OQS60_000255"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OQS60_000255"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS2386121.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OQS60_000256"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OQS60_000256"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS2386122.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OQS60_000257"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OQS60_000257"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS2386123.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OQS60_000258"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OQS60_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS2386124.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="OQS60_000259"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="OQS60_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS2386125.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OQS60_000260"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OQS60_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS2386126.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OQS60_000261"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OQS60_000261"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS2386127.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="OQS60_000262"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="OQS60_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS2386128.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="OQS60_000263"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="OQS60_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS2386129.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="OQS60_000264"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="OQS60_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="OQS60_000265"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="OQS60_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OQS60_000266"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OQS60_000266"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS2386130.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="OQS60_000267"
     CDS             complement(36855..36899)
                     /locus_tag="OQS60_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386131.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="OQS60_000268"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="OQS60_000268"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS2386132.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="OQS60_000269"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="OQS60_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS2386133.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="OQS60_000270"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="OQS60_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS2386134.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OQS60_000271"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OQS60_000271"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS2386135.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OQS60_000272"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OQS60_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS2386136.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OQS60_000273"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OQS60_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS2386137.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OQS60_000274"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OQS60_000274"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS2386138.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OQS60_000275"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OQS60_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS2386139.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OQS60_000276"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OQS60_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS2386140.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OQS60_000277"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OQS60_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS2386141.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OQS60_000278"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OQS60_000278"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS2386142.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OQS60_000279"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OQS60_000279"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS2386143.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OQS60_000280"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OQS60_000280"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS2386144.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OQS60_000281"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OQS60_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS2386145.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OQS60_000282"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OQS60_000282"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS2386146.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OQS60_000283"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OQS60_000283"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS2386147.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OQS60_000284"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OQS60_000284"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS2386148.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OQS60_000285"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OQS60_000285"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS2386149.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OQS60_000286"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OQS60_000286"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS2386150.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OQS60_000287"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OQS60_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS2386151.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OQS60_000288"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OQS60_000288"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS2386152.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OQS60_000289"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OQS60_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS2386153.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16855 c        14997 g        14510 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggtttcggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcccccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acagacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacg
//
LOCUS       DAJVYM010000005        59841 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVYM010000005 DAJVYM010000000
VERSION     DAJVYM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.005"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..262)
                     /locus_tag="OQS60_000290"
     CDS             complement(<1..262)
                     /locus_tag="OQS60_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2386154.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OQS60_000291"
     CDS             369..665
                     /locus_tag="OQS60_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2386155.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OQS60_000292"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OQS60_000292"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS2386156.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OQS60_000293"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OQS60_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS2386157.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OQS60_000294"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OQS60_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS2386158.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OQS60_000295"
     CDS             complement(3472..3645)
                     /locus_tag="OQS60_000295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386159.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OQS60_000296"
     CDS             3673..3756
                     /locus_tag="OQS60_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS2386160.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OQS60_000297"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OQS60_000297"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS2386161.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OQS60_000298"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OQS60_000298"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS2386162.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OQS60_000299"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OQS60_000299"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS2386163.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OQS60_000300"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OQS60_000300"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS2386164.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OQS60_000301"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OQS60_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS2386165.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OQS60_000302"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OQS60_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS2386166.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OQS60_000303"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OQS60_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS2386167.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OQS60_000304"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OQS60_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS2386168.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OQS60_000305"
     CDS             14052..15254
                     /locus_tag="OQS60_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS2386169.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OQS60_000306"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OQS60_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OQS60_000307"
     CDS             16953..18560
                     /locus_tag="OQS60_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS2386170.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OQS60_000308"
     CDS             18822..20444
                     /locus_tag="OQS60_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS2386171.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OQS60_000309"
     CDS             20810..21877
                     /locus_tag="OQS60_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS2386172.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OQS60_000310"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OQS60_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS2386173.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OQS60_000311"
     CDS             24609..25733
                     /locus_tag="OQS60_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2386174.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OQS60_000312"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OQS60_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OQS60_000313"
     CDS             26076..26435
                     /locus_tag="OQS60_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS2386175.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OQS60_000314"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OQS60_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS2386176.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OQS60_000315"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OQS60_000315"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS2386177.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OQS60_000316"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OQS60_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS2386178.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OQS60_000317"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OQS60_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS2386179.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OQS60_000318"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OQS60_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS2386180.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OQS60_000319"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OQS60_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS2386181.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OQS60_000320"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OQS60_000320"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS2386182.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OQS60_000321"
     CDS             complement(32630..33679)
                     /locus_tag="OQS60_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS2386183.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OQS60_000322"
     CDS             33811..35028
                     /locus_tag="OQS60_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2386184.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OQS60_000323"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OQS60_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS2386185.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OQS60_000324"
     CDS             complement(35662..36327)
                     /locus_tag="OQS60_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS2386186.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OQS60_000325"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OQS60_000325"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS2386187.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OQS60_000326"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OQS60_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS2386188.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OQS60_000327"
     CDS             complement(39167..39793)
                     /locus_tag="OQS60_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS2386189.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OQS60_000328"
     CDS             39934..40293
                     /locus_tag="OQS60_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS2386190.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OQS60_000329"
     CDS             complement(40296..40565)
                     /locus_tag="OQS60_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS2386191.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OQS60_000330"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OQS60_000330"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS2386192.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OQS60_000331"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OQS60_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS2386193.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OQS60_000332"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OQS60_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS2386194.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OQS60_000333"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OQS60_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS2386195.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OQS60_000334"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OQS60_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS2386196.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OQS60_000335"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OQS60_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS2386197.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OQS60_000336"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OQS60_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS2386198.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OQS60_000337"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OQS60_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS2386199.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OQS60_000338"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OQS60_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS2386200.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OQS60_000339"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OQS60_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS2386201.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OQS60_000340"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OQS60_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS2386202.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OQS60_000341"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OQS60_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS2386203.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OQS60_000342"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OQS60_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OQS60_000343"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OQS60_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS2386204.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OQS60_000344"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OQS60_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS2386205.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="OQS60_000345"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="OQS60_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCS2386206.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="OQS60_000346"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="OQS60_000346"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS2386207.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="OQS60_000347"
     CDS             complement(57808..58248)
                     /locus_tag="OQS60_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS2386208.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59841
                     /gene="ggt"
                     /locus_tag="OQS60_000348"
     CDS             58368..>59841
                     /gene="ggt"
                     /locus_tag="OQS60_000348"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS2386209.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14594 a        15113 c        16139 g        13995 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtgg
    59581 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    59641 ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg aacatttacg
    59701 ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcatcgatgt
    59761 caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt cctggcggga
    59821 gtcggattat taccaccgtg c
//
LOCUS       DAJVYM010000006        58073 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVYM010000006 DAJVYM010000000
VERSION     DAJVYM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.006"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..160
                     /locus_tag="OQS60_000349"
     CDS             <1..160
                     /locus_tag="OQS60_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS2386210.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="OQS60_000350"
     CDS             complement(198..1259)
                     /locus_tag="OQS60_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS2386211.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="OQS60_000351"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="OQS60_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS2386212.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="OQS60_000352"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="OQS60_000352"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS2386213.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OQS60_000353"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OQS60_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS2386214.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OQS60_000354"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OQS60_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS2386215.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OQS60_000355"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OQS60_000355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS2386216.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OQS60_000356"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OQS60_000356"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS2386217.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OQS60_000357"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OQS60_000357"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS2386218.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OQS60_000358"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OQS60_000358"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS2386219.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OQS60_000359"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OQS60_000359"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS2386220.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OQS60_000360"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OQS60_000360"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS2386221.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="OQS60_000361"
     CDS             complement(14497..14649)
                     /locus_tag="OQS60_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386222.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="OQS60_000362"
     CDS             14667..14858
                     /locus_tag="OQS60_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS2386223.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="OQS60_000363"
     CDS             15169..15687
                     /locus_tag="OQS60_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS2386224.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="OQS60_000364"
     CDS             15703..16242
                     /locus_tag="OQS60_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS2386225.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OQS60_000365"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OQS60_000365"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS2386226.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OQS60_000366"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OQS60_000366"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS2386227.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="OQS60_000367"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="OQS60_000367"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS2386228.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="OQS60_000368"
     CDS             complement(20978..21193)
                     /locus_tag="OQS60_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS2386229.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="OQS60_000369"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="OQS60_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS2386230.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OQS60_000370"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OQS60_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS2386231.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OQS60_000371"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OQS60_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS2386232.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OQS60_000372"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OQS60_000372"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS2386233.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OQS60_000373"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OQS60_000373"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS2386234.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OQS60_000374"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OQS60_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS2386235.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OQS60_000375"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OQS60_000375"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS2386236.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OQS60_000376"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OQS60_000376"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS2386237.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OQS60_000377"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OQS60_000377"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS2386238.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OQS60_000378"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OQS60_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS2386239.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="OQS60_000379"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="OQS60_000379"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS2386240.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OQS60_000380"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OQS60_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS2386241.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OQS60_000381"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OQS60_000381"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS2386242.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OQS60_000382"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OQS60_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS2386243.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OQS60_000383"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OQS60_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS2386244.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OQS60_000384"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OQS60_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS2386245.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OQS60_000385"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OQS60_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS2386246.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OQS60_000386"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OQS60_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS2386247.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OQS60_000387"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OQS60_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS2386248.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OQS60_000388"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OQS60_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS2386249.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OQS60_000389"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OQS60_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS2386250.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OQS60_000390"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OQS60_000390"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS2386251.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="OQS60_000391"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="OQS60_000391"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS2386252.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="OQS60_000392"
     CDS             49418..50272
                     /locus_tag="OQS60_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2386253.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="OQS60_000393"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="OQS60_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS2386254.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="OQS60_000394"
     CDS             complement(51735..52874)
                     /locus_tag="OQS60_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS2386255.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OQS60_000395"
                     /pseudo
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OQS60_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54060..55421
                     /locus_tag="OQS60_000396"
     CDS             54060..55421
                     /locus_tag="OQS60_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS2386256.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="OQS60_000397"
     CDS             55418..55936
                     /locus_tag="OQS60_000397"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS2386257.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="OQS60_000398"
     CDS             55936..56256
                     /locus_tag="OQS60_000398"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS2386258.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="OQS60_000399"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="OQS60_000399"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS2386259.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..>58073
                     /locus_tag="OQS60_000400"
     CDS             57075..>58073
                     /locus_tag="OQS60_000400"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS2386260.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGD"
BASE COUNT        14186 a        15721 c        14478 g        13688 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacgatttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacacgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttacgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccaaca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gat
//
LOCUS       DAJVYM010000007        57433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVYM010000007 DAJVYM010000000
VERSION     DAJVYM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.007"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="OQS60_000401"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="OQS60_000401"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS2386261.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3105)
                     /locus_tag="OQS60_000402"
     CDS             complement(1498..3105)
                     /locus_tag="OQS60_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS2386262.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3583..3669)
                     /locus_tag="OQS60_000403"
     tRNA            complement(3583..3669)
                     /locus_tag="OQS60_000403"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3633..3635),aa:Leu,seq:gag)
     gene            complement(3684..4016)
                     /gene="secG"
                     /locus_tag="OQS60_000404"
     CDS             complement(3684..4016)
                     /gene="secG"
                     /locus_tag="OQS60_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS2386263.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="OQS60_000405"
     CDS             complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="OQS60_000405"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS2386264.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5574..6422)
                     /gene="folP"
                     /locus_tag="OQS60_000406"
     CDS             complement(5574..6422)
                     /gene="folP"
                     /locus_tag="OQS60_000406"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS2386265.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="OQS60_000407"
     CDS             complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="OQS60_000407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS2386266.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="OQS60_000408"
     CDS             complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="OQS60_000408"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS2386267.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9301..9594
                     /gene="yhbY"
                     /locus_tag="OQS60_000409"
     CDS             9301..9594
                     /gene="yhbY"
                     /locus_tag="OQS60_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS2386268.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9750..10226)
                     /gene="greA"
                     /locus_tag="OQS60_000410"
     CDS             complement(9750..10226)
                     /gene="greA"
                     /locus_tag="OQS60_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS2386269.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10474..11907
                     /gene="dacB"
                     /locus_tag="OQS60_000411"
     CDS             10474..11907
                     /gene="dacB"
                     /locus_tag="OQS60_000411"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS2386270.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="OQS60_000412"
     CDS             complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="OQS60_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS2386271.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13281..14246)
                     /locus_tag="OQS60_000413"
     CDS             complement(13281..14246)
                     /locus_tag="OQS60_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS2386272.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="OQS60_000414"
     CDS             complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="OQS60_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS2386273.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="OQS60_000415"
     CDS             complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="OQS60_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS2386274.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15221..16192
                     /gene="ispB"
                     /locus_tag="OQS60_000416"
     CDS             15221..16192
                     /gene="ispB"
                     /locus_tag="OQS60_000416"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS2386275.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16421..16699
                     /gene="sfsB"
                     /locus_tag="OQS60_000417"
     CDS             16421..16699
                     /gene="sfsB"
                     /locus_tag="OQS60_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS2386276.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16747..18006)
                     /gene="murA"
                     /locus_tag="OQS60_000418"
     CDS             complement(16747..18006)
                     /gene="murA"
                     /locus_tag="OQS60_000418"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS2386277.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="OQS60_000419"
     CDS             complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="OQS60_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS2386278.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="OQS60_000420"
     CDS             complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="OQS60_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS2386279.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="OQS60_000421"
     CDS             complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="OQS60_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS2386280.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="OQS60_000422"
     CDS             complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="OQS60_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS2386281.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="OQS60_000423"
     CDS             complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="OQS60_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS2386282.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="OQS60_000424"
     CDS             complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="OQS60_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS2386283.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21787..22764
                     /locus_tag="OQS60_000425"
     CDS             21787..22764
                     /locus_tag="OQS60_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS2386284.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22778..23764
                     /gene="kdsD"
                     /locus_tag="OQS60_000426"
     CDS             22778..23764
                     /gene="kdsD"
                     /locus_tag="OQS60_000426"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS2386285.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23785..24351
                     /gene="kdsC"
                     /locus_tag="OQS60_000427"
     CDS             23785..24351
                     /gene="kdsC"
                     /locus_tag="OQS60_000427"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS2386286.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24348..24923
                     /gene="lptC"
                     /locus_tag="OQS60_000428"
     CDS             24348..24923
                     /gene="lptC"
                     /locus_tag="OQS60_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS2386287.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24892..25449
                     /gene="lptA"
                     /locus_tag="OQS60_000429"
     CDS             24892..25449
                     /gene="lptA"
                     /locus_tag="OQS60_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS2386288.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25456..26181
                     /gene="lptB"
                     /locus_tag="OQS60_000430"
     CDS             25456..26181
                     /gene="lptB"
                     /locus_tag="OQS60_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS2386289.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26229..27662
                     /gene="rpoN"
                     /locus_tag="OQS60_000431"
     CDS             26229..27662
                     /gene="rpoN"
                     /locus_tag="OQS60_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS2386290.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27685..27972
                     /gene="hpf"
                     /locus_tag="OQS60_000432"
     CDS             27685..27972
                     /gene="hpf"
                     /locus_tag="OQS60_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS2386291.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28090..28581
                     /gene="ptsN"
                     /locus_tag="OQS60_000433"
     CDS             28090..28581
                     /gene="ptsN"
                     /locus_tag="OQS60_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS2386292.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28627..29481
                     /gene="rapZ"
                     /locus_tag="OQS60_000434"
     CDS             28627..29481
                     /gene="rapZ"
                     /locus_tag="OQS60_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS2386293.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29478..29750
                     /gene="npr"
                     /locus_tag="OQS60_000435"
     CDS             29478..29750
                     /gene="npr"
                     /locus_tag="OQS60_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS2386294.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29964..30596
                     /gene="yrbL"
                     /locus_tag="OQS60_000436"
     CDS             29964..30596
                     /gene="yrbL"
                     /locus_tag="OQS60_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS2386295.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="OQS60_000437"
     CDS             complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="OQS60_000437"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS2386296.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="OQS60_000438"
     CDS             complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="OQS60_000438"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS2386297.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="OQS60_000439"
     CDS             complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="OQS60_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS2386298.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34633..35562)
                     /locus_tag="OQS60_000440"
     CDS             complement(34633..35562)
                     /locus_tag="OQS60_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS2386299.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36237..40697
                     /gene="gltB"
                     /locus_tag="OQS60_000441"
     CDS             36237..40697
                     /gene="gltB"
                     /locus_tag="OQS60_000441"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS2386300.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40710..42128
                     /gene="gltD"
                     /locus_tag="OQS60_000442"
     CDS             40710..42128
                     /gene="gltD"
                     /locus_tag="OQS60_000442"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS2386301.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42312..42791
                     /locus_tag="OQS60_000443"
     CDS             42312..42791
                     /locus_tag="OQS60_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS2386302.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42808..43113
                     /locus_tag="OQS60_000444"
     CDS             42808..43113
                     /locus_tag="OQS60_000444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2386303.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43189..43653)
                     /locus_tag="OQS60_000445"
     CDS             complement(43189..43653)
                     /locus_tag="OQS60_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS2386304.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="OQS60_000446"
     CDS             complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="OQS60_000446"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS2386305.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44522..45211)
                     /locus_tag="OQS60_000447"
     CDS             complement(44522..45211)
                     /locus_tag="OQS60_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS2386306.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="OQS60_000448"
     CDS             complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="OQS60_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS2386307.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="OQS60_000449"
     CDS             complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="OQS60_000449"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS2386308.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="OQS60_000450"
     CDS             complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="OQS60_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS2386309.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49045..50367
                     /gene="dcuC"
                     /locus_tag="OQS60_000451"
     CDS             49045..50367
                     /gene="dcuC"
                     /locus_tag="OQS60_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS2386310.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50410..50907)
                     /gene="sspB"
                     /locus_tag="OQS60_000452"
     CDS             complement(50410..50907)
                     /gene="sspB"
                     /locus_tag="OQS60_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS2386311.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50913..51551)
                     /gene="sspA"
                     /locus_tag="OQS60_000453"
     CDS             complement(50913..51551)
                     /gene="sspA"
                     /locus_tag="OQS60_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS2386312.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51946..52338)
                     /gene="rpsI"
                     /locus_tag="OQS60_000454"
     CDS             complement(51946..52338)
                     /gene="rpsI"
                     /locus_tag="OQS60_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS2386313.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52354..52782)
                     /gene="rplM"
                     /locus_tag="OQS60_000455"
     CDS             complement(52354..52782)
                     /gene="rplM"
                     /locus_tag="OQS60_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS2386314.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53001..54128)
                     /gene="zapE"
                     /locus_tag="OQS60_000456"
     CDS             complement(53001..54128)
                     /gene="zapE"
                     /locus_tag="OQS60_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS2386315.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54322..54720
                     /locus_tag="OQS60_000457"
     CDS             54322..54720
                     /locus_tag="OQS60_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS2386316.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54874..56241
                     /gene="degQ"
                     /locus_tag="OQS60_000458"
     CDS             54874..56241
                     /gene="degQ"
                     /locus_tag="OQS60_000458"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS2386317.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56331..57398
                     /gene="degS"
                     /locus_tag="OQS60_000459"
     CDS             56331..57398
                     /gene="degS"
                     /locus_tag="OQS60_000459"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS2386318.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14267 a        15303 c        14253 g        13610 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtaggatca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaatcttct agcaaatttg ttgaatactg
     3121 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
     3181 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
     3241 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
     3301 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
     3361 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
     3421 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
     3481 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
     3541 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
     3601 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
     3661 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
     3721 gctggctgag tttgttcggt tttcgccggt gcactcagat tttcccattc gctaccttta
     3781 ttggttttgt tgctattgat gttacccagc accagactga tgataaagaa taacgttgcc
     3841 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
     3901 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
     3961 agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc gtacataatc
     4021 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
     4081 aatctttccc actgaagtgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
     4141 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
     4201 aaaaggcgac aatcgccgcc tttttagcca gttatttacc gttttaaacg gcttttactg
     4261 catcggcgat acggtgtgca aattcagtta cctgcgcttc gtcttcacct tctaccatca
     4321 cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg ctacccagcg
     4381 ccgcttcaac ttcggcggtc acagctttaa ctgactcatg ctcaagcgga tcgccgctac
     4441 ctgcggtgta acgcacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
     4501 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
     4561 cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct gcaccgatac
     4621 gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc gcgcgcgcaa
     4681 atggaattcc taactgtttc aacgccagct caagccccat gttactcatc aatgtaccca
     4741 cagcaccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
     4801 gatcaccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccgtcgccgt
     4861 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
     4921 cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg atagcgataa
     4981 cgttcgcccc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcgc
     5041 aatccaccac aatcttcagt tcgctgaggc taagttcgtt cgggaacgtg gctttgcaaa
     5101 actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt tctgccgaat
     5161 caacacagct gatctctttt tccatttccg cttcgatggc ctcttctacc gcatccggta
     5221 gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg ttatgcgatg
     5281 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
     5341 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
     5401 ctgactccag catatagcca gaaatacgcg tgtctttacc aataatgatc ttacgggagc
     5461 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
     5521 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
     5581 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
     5641 tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat gaccgcacag
     5701 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
     5761 tttcgtgaca tacctaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
     5821 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
     5881 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
     5941 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
     6001 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
     6061 gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc acggatgact
     6121 tctggtttgg atgtatctac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
     6181 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
     6241 gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt cgcatgtttc
     6301 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
     6361 aggatcccca ttacgtgagg atggctaagg tccagtgaag tgccctgggc aaagagtttc
     6421 atggtgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
     6481 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
     6541 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
     6601 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
     6661 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
     6721 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
     6781 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
     6841 gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc gcgtacagca
     6901 gtggacccaa tttctcagag aagccccact gagtcaccat gttacgcgcc aggttggtcg
     6961 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag atgatctctt
     7021 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
     7081 tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
     7141 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
     7201 cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag cgacgttccg
     7261 cacccatcat gattttatct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
     7321 tgccacgagc agcgaacagt gccgcttcgt ttaccaggtt cgccaggtca gcaccggaga
     7381 aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat ggtacgcgac
     7441 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagcca accacaacct
     7501 gacggtcgaa acggccagga cgcagcaggg ccgggtcaag aacgtccgga cggttagtcg
     7561 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
     7621 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
     7681 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
     7741 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
     7801 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc gttttaccgg
     7861 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
     7921 ggctcggctc gcgcagatat tcaaccagtt cagcaacttc ttcttttgct tcgtcgcagc
     7981 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
     8041 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
     8101 acacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
     8161 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
     8221 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
     8281 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
     8341 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
     8401 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
     8461 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
     8521 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
     8581 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
     8641 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
     8701 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
     8761 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
     8821 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
     8881 cgccctgaag aaagtctaca ccaacgatag gatccatagg taaaagatcg caagcgatga
     8941 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
     9001 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
     9061 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
     9121 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
     9181 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
     9241 atatatggga gatggcggta gaatgaccca ttttcaatcc caacgtaagc aaaaaatacg
     9301 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
     9361 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
     9421 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc
     9481 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
     9541 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
     9601 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
     9661 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
     9721 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
     9781 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
     9841 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
     9901 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
     9961 ccaaaaataa cgcgcccatt gttaggcatt ttggtgacat caatcacctg cgcgtttgac
    10021 agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc acgagctgcg
    10081 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    10141 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    10201 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    10261 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    10321 aaaaaaatac cgacccaggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    10381 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    10441 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    10501 ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta cattactcaa
    10561 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    10621 attgattacc acagtcagca gatggcgctg cctgccagta cccagaaagt gattactgcg
    10681 ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct tgaaaccaaa
    10741 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    10801 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    10861 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    10921 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc cgccatagtt
    10981 gaccgcaact gtttctccgt ctcgctctac agtgcccaaa aacccggtga tatggccttt
    11041 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccgt
    11101 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    11161 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    11221 gatggagcca gttatgccgg tgcaattctg aaagatgagt taaaacaggc gggtatcacc
    11281 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    11341 aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc ggataacatg
    11401 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtacc tggtacatgg
    11461 cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    11521 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    11581 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    11641 ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    11701 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    11761 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc tggctatgca
    11821 gtagaacctg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    11881 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggctt
    11941 cagattattg acaaagtgcg ctttgtttat gccggatgcg gcgtaaacgc cttatccggc
    12001 ctacaaaatc gtgcaaattc aacatattgc aattctcttg taggcctgat aagcgtagcg
    12061 catcaggctg atttggcgtt tatcatcagt gattaacgct tgtaaatgaa ctcaacgcct
    12121 tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct
    12181 tcaagctgct ggcgatgata atcgtcccac atgaattcga ctttctctgg ctgtttcgct
    12241 tcttcagcct gcacgaccgg gttttcaagg ataaaggtca tcacatccca gcagagatct
    12301 ttcacgccca gtccgctcgc cgcagagatc agataatatt tatcttccca gcccagcgct
    12361 tcagcaatcg ctttcgcttt ctcttcagct tctgccttat ccagcagatc gatcttattg
    12421 aacactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata
    12481 ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc
    12541 aacaggacgc ggcaacgttc caggtgcttc aggaagcgga tacccagacc tgcgccttcc
    12601 gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt
    12661 cgtaccacac ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta
    12721 gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg
    12781 acgtcagcca gcagcatcag ctccagcagc agctcacgct tatcacccgg cgtaccgttg
    12841 gttttctgcc gtggtgtacg gttaacggac gatttgaaac gggtattgcc cagaccgtgc
    12901 cagccgccct tagcaaccag cagacgctga ccgtgtttag tcatatcgcc catggtttca
    12961 ccagtaccct ggtcgattac acgcgtacct accggcactt taatcgtcac gtctttaccg
    13021 cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc gctctgcacg gaaagatttt
    13081 tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg cttccatcca tacgtcacca
    13141 ccatcaccac cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag
    13201 ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac
    13261 ttcattttat tctccataaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc
    13321 ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg
    13381 acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag
    13441 gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg
    13501 ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaat
    13561 gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact
    13621 ccaggctttg ccagagggaa gagcgcaatt gtacataaag tgtacagtaa aaacaggatc
    13681 tgcggtgagg ccagccgacg caataaaacc ttttgcgcca cgccataact cacccaaacc
    13741 gtcgccgcac cgaccccaaa gataactccc caggtgtaat cggtgagctt tgtaaatatc
    13801 tcgaccagac tggtgttaaa aaacatcacc aggccgctca ggagcatcaa tgcccctaca
    13861 acctgagtgc tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact
    13921 ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg
    13981 aacaggatga agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt
    14041 ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg
    14101 ccaatactcg ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc
    14161 acctgcttca ttgcgattgg caacgccccc cagcaaattg ctgtggtgag cgccaaaaga
    14221 atgccaatgc ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa
    14281 cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg
    14341 gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta
    14401 cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg
    14461 tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg
    14521 atgatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct
    14581 tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc
    14641 tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatgac
    14701 cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac
    14761 gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt
    14821 tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg
    14881 cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac
    14941 cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc
    15001 agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga
    15061 caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg
    15121 tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt
    15181 tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa
    15241 tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc
    15301 cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat
    15361 tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt
    15421 caccattgct gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt
    15481 ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc
    15541 cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg
    15601 ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac gtcatcgcag aaggtgaagt
    15661 tctgcaactg atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat
    15721 ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg
    15781 ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt
    15841 ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaatgt
    15901 cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg
    15961 cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct
    16021 tctggaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg
    16081 tgccgaggaa gaagcagaca aagccatcgc agcattacag gtgctcccgg acaccccttg
    16141 gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc
    16201 cttcatcccg cgcagtgcgc gggatgattc cagtaaattc cataaaaaca ctaattcagc
    16261 gctataacac ctgaaaaaaa agggcgatga atctttatat attctgaata ttcacactct
    16321 ttacaggaac tttttagagc aataggccat caggagtata gtgatgcccg acagaagaag
    16381 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    16441 ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacgtcaa tggcggcgga
    16501 gtctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    16561 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    16621 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    16681 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    16741 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    16801 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    16861 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    16921 gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt cggcatgcgc
    16981 gcccatacgg ctaagctccg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    17041 gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    17101 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    17161 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcgtcacgca gtttcgccag
    17221 cacggcgtcg agagtatctg gctgcgcgtt acggcagata attttgccgc gagaaatcgc
    17281 cgccgccacc aggaaagtac cggtttcgat acgatccggt agcacgcgat agacaccgcc
    17341 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    17401 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcattttc
    17461 aataatcgtg gtaccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    17521 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    17581 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    17641 aaccggacgc gcaccgatcg tacaaccgcc aggcagtgaa acttgcccct gaccaaagcg
    17701 cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca gatcgtaagg
    17761 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    17821 cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt ttgggacgtt
    17881 ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca gagcagcatt
    17941 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    18001 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    18061 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    18121 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    18181 ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct gaaagtggct
    18241 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    18301 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    18361 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    18421 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    18481 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    18541 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    18601 tgccagtcca cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    18661 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    18721 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    18781 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    18841 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    18901 gcaatcatgt cgtaagcctg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    18961 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    19021 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    19081 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    19141 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    19201 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    19261 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    19321 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    19381 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    19441 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    19501 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    19561 ttagtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    19621 aaaccgacgt ttaatgccag atattgttcc cccagcaggc cggaagtacg aatgctcagc
    19681 gaactggtat ctggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    19741 taggttttcg ggtccagcgt aatatccgcc acccgaccca caacaacgcc accaatactg
    19801 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    19861 ggttcagtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    19921 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    19981 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    20041 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg tgtcgtagcc
    20101 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    20161 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    20221 aatgcctttc caactgacgc cgaccagaga tccgccccag atccccacgg cgacgaaaat
    20281 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    20341 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    20401 gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg caaccaccgg
    20461 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcactata
    20521 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    20581 cacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    20641 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    20701 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    20761 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    20821 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgcct
    20881 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    20941 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    21001 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    21061 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    21121 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    21181 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcgggt agttgggtat
    21241 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    21301 actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga gacatcgccg
    21361 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    21421 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    21481 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    21541 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    21601 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    21661 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    21721 gaatccgtta cggctttctg aaaatcttca gcggaccgac gagtatacct gaagaaagga
    21781 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    21841 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    21901 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    21961 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    22021 atcaatattt tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    22081 gatgttctgc gtcgtgaatt acccttaatg ttgttggtca gcgtagtggc cggttccgta
    22141 ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct ggctgtgcta
    22201 tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac tgacagcctg
    22261 accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc gtttttatgg
    22321 ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga taacgccacg
    22381 gtgctggcga attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc
    22441 ggaaccagcc tgccggaact ggcaaccgca atagcggggg ttcgcaaagg tgaaaacgac
    22501 attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta
    22561 cccgcgctga taacgccagg agagattgag ccattggcgt acagtcgtga ctacagcgtg
    22621 atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc
    22681 cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat gttgtactgg
    22741 ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc
    22801 gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc
    22861 ggagcttgat caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg
    22921 taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc
    22981 cgcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg aagccgcgca
    23041 tggtgattta ggcatggtca ccccacagga tgtggtgatt gctatctcta actcaggtga
    23101 atccagcgaa atcacggcct taattccagt gcttaagcgt cttcacgtac cgttaatctg
    23161 catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa
    23221 agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca ccgccacgct
    23281 ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga
    23341 ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga
    23401 tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc
    23461 gttgctggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat
    23521 gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt
    23581 tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgtgtgc gccctggcat
    23641 tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc
    23701 cgatggcgac catttactcg gagtgttaca tatgcatgat ttactgcgtg caggcgtagt
    23761 gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac
    23821 ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca ttcgtctgct gatcctcgat
    23881 gtcgatggcg tactgtcaga tggcctgatt tatatgggca ataatggcga agagctgaaa
    23941 gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc
    24001 gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc
    24061 actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct gctggaaaaa
    24121 ctggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga ctggccggta
    24181 atggaaaaag tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt gatcccgcgc
    24241 gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt ttgcgactta
    24301 ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca
    24361 gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggc attgatatgg
    24421 ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg
    24481 agcatacgga cacgctcgtc tataacccag aaggggcact aagctatcga ttgattgctc
    24541 aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca
    24601 cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca
    24661 atgaccggat gctctattta tatggacacg ttgaagtcaa cgcacttgtg ccaaactctc
    24721 aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct
    24781 ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg
    24841 gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc
    24901 aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt
    24961 ccggcatttg ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa
    25021 tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt cacccagggc
    25081 accatcaaaa ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa
    25141 gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga caacggtaaa
    25201 cccgttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg
    25261 acgggtaatg cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac
    25321 ctggtaaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt aaccaccgtt
    25381 ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc acagaagaag
    25441 ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc
    25501 cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg
    25561 gggccaaacg gtgccggtaa gaccaccact ttctacatgg ttgtaggcat tgtgccgcgc
    25621 gatgcgggca acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc
    25681 gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg cctcagcgtt
    25741 tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa
    25801 gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg
    25861 cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat
    25921 ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgac
    25981 attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat cactgaccac
    26041 aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg
    26101 atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt
    26161 ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga
    26221 ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac
    26281 agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caggagctac
    26341 agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca
    26401 cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa caaaaagaga
    26461 tgccggaaga gctgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat
    26521 ccggcaccag cggtgactac attgacgatg agctgccggt ctatcagggc gaaacgacgc
    26581 agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc gacactgacc
    26641 gcgctattgc tacctctatc gtcgatgccg ttgatgacat cggttatctg actgtcccgc
    26701 tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgag gttgaagccg
    26761 tccttaagcg gatccaacgg tttgatccgg tcggtgtagc ggcaaaagat ctgcgtgact
    26821 gcctgctaat ccaactctcc caattcgata aaaccacgcc gtggctggaa gaggccagac
    26881 tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact ttaatgcgcg
    26941 tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc
    27001 cgcgccccgg gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg
    27061 tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt ccgcgtctgc
    27121 aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caacgatggt gacagccagt
    27181 ttatccgcag caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg
    27241 atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag
    27301 gtgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg
    27361 aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg
    27421 aactgaagta tttcttttcc agtcacatca ataccgaggg cggcggcgaa gcttcctcca
    27481 cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga
    27541 gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg
    27601 ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt
    27661 gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc
    27721 accgaggcac tgcgcgaatt tgttacagcc aaatttgcca aacttgagca atattttgac
    27781 cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag tcacccacac ctcagatgca
    27841 acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct
    27901 gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa agataaactg
    27961 aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac
    28021 gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg caggttctta
    28081 ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa
    28141 tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat catcagcgag
    28201 ctggcggcga aacaacttag cctgccgcct caggtggttt ttgaagctat cctgacgcgg
    28261 gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaactggaa
    28321 gaagatactc tgcgcgccgt tggcgttttc gttcagctcg aaacgcctat agctttcgat
    28381 gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgtcggc agaccaaact
    28441 aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc
    28501 cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa
    28561 ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa
    28621 cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg
    28681 cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc
    28741 gatctggctc gaaccctggc cgatcgcgag atttctgccg ccgtcagcat tgatgttcgt
    28801 aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc
    28861 tcaccgcaac tcctgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac
    28921 acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa
    28981 gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa
    29041 atgtccgttc acgagctggc tgaaatgctg cgtacccgtc tgctgggtaa acgtgaacgc
    29101 gaactgacca tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat
    29161 tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact gcgtccaatg
    29221 acaggtcttg ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt
    29281 atctaccaga cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt
    29341 agctacttga cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca
    29401 gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcgtacg
    29461 ctggaaaaac gtaaaccatg accgtcaagc aaactgttga aatcacaaac aagctgggca
    29521 tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc
    29581 tgttacgtaa tgacgaaggc accgaggccg aagccaacag cgttattgcg ctgctgatgt
    29641 tggattctgc caaaggacgg cagattgaag ttgaagcgac cggcccacag gaagaggaag
    29701 cactggccgc cgttatcgcc ctctttaact ctggttttga tgaagattaa tcttcatcac
    29761 tttgacatac aactcccttc aaactccccc cgacaataag aaaatcacgt actgaaatcg
    29821 ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag aaccttaaga
    29881 aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact ggagatcgtc
    29941 agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc ctgggcaccg
    30001 gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc aagattgtct
    30061 accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac tacgcgcatc
    30121 ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc gttgagacgg
    30181 attgcggaac cggttatgtc tacgatgtga ttgcagattt tgacggcaaa ctctccatta
    30241 cgctgaccga atttgctgaa caatgccgtt acgaagaaga cattgcacaa ctgcgccagc
    30301 tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc
    30361 cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc gtctgcgaca
    30421 acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt tgtctgcgta
    30481 agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt gccctgcaga
    30541 aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca
    30601 gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt aaaatccacg
    30661 cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt ggattaggta
    30721 atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat
    30781 aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg atattcaggt
    30841 aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt aatccagctt
    30901 ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagatttttg gccgtctgct
    30961 gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc gccagggctt
    31021 tctcaatgga agcgacatca aagccccagt gctcagaaaa tttctgatct tctgcggcaa
    31081 tcaccgccag tcccatccac ggcgagattt gatccatact gacccagtca gaatgtgcca
    31141 cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg ctcgaccatt accgctgaga
    31201 agggaacagg cgcaacacta aacaacgcga tgcccccgcc ccagaaaacg gcgaggacaa
    31261 ccattaacag caaaagaaaa cggcgaacga aactaaacac cgttaagcgg cttttactca
    31321 ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg cttctgcaat
    31381 gttctgcgcc agcatatatg ctggggtggt gacaatctta ttgtcttcat caaccacgat
    31441 atcatcgaca ggacagggca catgctccgc gcccatctct tccagcactt ctgcggtatc
    31501 gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca gcatcgccgg
    31561 ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt
    31621 taattcacgg tcaacggtgc attcgctacc aagagtggca aaattgctta aattcttcgc
    31681 cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg cctgggccag
    31741 aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacgttgc gcgtttccgt
    31801 catcgcttcg ccagttaaat ggttgataac atcaacctgc tgcttatccg gtgcaaagca
    31861 gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt catgaatttc
    31921 agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca ttgtgatcat
    31981 cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg catcacacat
    32041 ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt gcggcctgaa
    32101 aaacagtgct gtgcccttgt aactcatcat aataatttac ggcgcagcca agatttccct
    32161 ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta gtggcttttg
    32221 ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc tcttcaatcc
    32281 attcaccgac gttatcttcc caggcaggaa ggtcaggaga ctgaatttgc tgacccagct
    32341 gttgcagatg gcgtaacccc actgaccccg ccgcaccttt aattttatgt ccttcctcaa
    32401 caatgccttt tttatcctgc gccgtcagat tcgactccag cacgctgaca tagcccggca
    32461 tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca agttcgagat
    32521 actgttccag catgggaata tcgagcaatg cttctgattt actgttctct tctgtcgtca
    32581 ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg gttagcgccg
    32641 gaacagaaag cggcttactc agcacatcat ccattccagc attgaggtac tcttgtttgt
    32701 ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctctcgc ggataacgct
    32761 tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata tccagtaaca
    32821 ccaggtcgta ttcgcccggt ttaaacatct ctagcgccgc cttgccggtc atggcgacat
    32881 caacgctgtt acctaatttt tccagcacag aacgcgcaac aatcacgttc agttcaatgt
    32941 cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca aacgcatcat
    33001 cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct
    33061 gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga gaaacggcca
    33121 gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc tggtaataca
    33181 tggcgaaaat tttatccagc tcatcctgcg gaatgccgat accagagtct tccacttcaa
    33241 aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct tgctgggtga
    33301 atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc ccgtcggtaa
    33361 tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcgcaatcct ttttgttgcg
    33421 cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca accggttgat
    33481 tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg ttaaagatat
    33541 tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc tcggcggtga
    33601 gttcggtatc cagcagaatg cggctcagac cgacgatacc gttcagcggt gtacgcaatt
    33661 cgtgactgat ggtggagata aacgtcgttt tgtcgcggct ggcccgttca agcgcatcct
    33721 gataccgctt acgctcggta atgtctcgac caaagcccat caaaccgtga cgtttaccca
    33781 cgcggtcgta gtacggcact ttacggattt caaagcaggc tttgcgcccg tccgggtaat
    33841 ccagccactg ttcataggtc agtgacacat tatgacggaa cactttttca tcggtttcaa
    33901 tgacttttgc ggcggcttcc ggtgagtaaa catcaacagg tttcaggtga accagttgtt
    33961 tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac tctttatctt
    34021 cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag aatgaggatt
    34081 gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc agttggccga
    34141 aggtttcctg cagttctgct tccgcttttt cacgaacggc gatttcctga tttagctggg
    34201 caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt
    34261 tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg accaccgata
    34321 gaaagtagac cgcccacggc gtaatcaaca aaccaaagaa gatagaacga ataacatcaa
    34381 tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca atggcaagaa
    34441 cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc atcatcaggt
    34501 caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt cacgacaacc
    34561 tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa atgcggaatt
    34621 cccctcaacg acctactccg ttggaggtag ccacggacgt cccagcgccg atccctgtac
    34681 gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat tttcgcacca
    34741 cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt agatcacttc
    34801 cggtggcgta tggcgaatca tttctccggc agtgagcgtg taatcctcca gttcaatacc
    34861 gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa tatgcaacgg
    34921 atgtagcttt ataccgtcaa caccggtttc cactacccgc tccagcgttt gcagacattc
    34981 agccagccct tcgcctggca gaccgacaat taaatgcgag cacaccttca gcccgcgctg
    35041 gcgtgccagc tgcgtcgtgc gctgataaca ggcgaaatca tgaccgcgat taatgcgatg
    35101 tagcgttttg tcgtgcacgg tttgcagccc cagctccagc cacacttcgt agccctggtc
    35161 tttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc gcgtgccgac
    35221 acacaaaccg acaatattgg cctggctcac cgcctgttga tacatcgaac gcagcacctg
    35281 aacttcggca aaggtgctgg tatacgcctg gaaataggcg aggtaacgtt tcgcgcgatt
    35341 aattaaattc gcctgatgcg ccagctgttc ggcaatggaa cgatgctgct gcgcttcgtc
    35401 ggcaaacgag gcaacattac agaaggtgca gccgccgcgc ccgatggtgc cgtcgcgatt
    35461 cgggcaactg aaaccgccgt gcagcgtcag cttatgcacc ttttgcccat aacgacgggt
    35521 gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat agaccgcgcc
    35581 ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgacct ggatcaatcg
    35641 tcccggcccg cttttatcaa ctgcataatc aatcaaaatt accgaaattt catgcataat
    35701 cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat ggattttagg
    35761 caaaaacagt agcatgaaac gtcattacca attaatgcag tataaaatgc tggttttctc
    35821 gtcaattcaa ggcaggataa gggttaacac gcctttatga cagtcaggaa ttgactgttt
    35881 ctctaacgac ttccctttta gccttaaaga taaaatctat tttaatttca gccatttaat
    35941 aaagaatttt gcgctaaagc acatttctgt accaataagc ttgccatttg acctgtatca
    36001 gctttcccga taagttggaa atccgctgga agctttctgg atgagcagcc tgctcatcat
    36061 atttatgcag taattgagat cccctcttca ccgtattaac cgatgcgaaa aggacaacaa
    36121 gggggcgaat gcgaggcgcg cgtatgacac gcaacccccg tcgccacgct ctttctgtac
    36181 ccgtgcgcag cggttcggta gtggggttcc cgcagagcct gggggaggtt cacgatatgt
    36241 tgtacgataa atcccttgag agggataact gtggtttcgg cctgatcgcc cacatagaag
    36301 gcgaacctag ccacaaggta gtgcgtactg caatacacgc actggcccgc atgcagcacc
    36361 gtggcgcgat tctcgccgat ggtaaaaccg gtgacggttg cggcttgctg ttacaaaaac
    36421 cggatcgctt ttttcgcatc gttgcgcagg agcgcggctg gcgtttagcg aaaaactacg
    36481 ccgtcgggat gctattcctg aataaagatc ctgaactcgc cgctgccgca cgccgcatcg
    36541 ttgaagaaga actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat gtccccacta
    36601 acgaaggcgt actgggtgaa atcgccctct cctctctgcc ccgcattgag caaatttttg
    36661 tgaacgcccc ggcaggctgg cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc
    36721 gccgcattga aaagcgtctc gaagccgaca aagacttcta cgtctgtagc ctatcgaatc
    36781 tggtgaacat ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt tatctggatc
    36841 ttgcggacct gcgtctggaa tcggccattt gcctgttcca ccagcgcttc tccactaaca
    36901 ccgtaccgcg ctggccgctg gcgcaaccgt tccgctatct ggcgcataac ggtgaaatca
    36961 acaccatcac tggtaaccgc caatgggcgc gtgcgcgtac ctataaattc cagacgccgc
    37021 ttatccctga tctgcacgac gccgcaccgt tcgtcaacga aaccggctct gactccagtt
    37081 cgatggataa catgctggaa ctcctgctgg caggcgggat ggatatcatc cgcgccatgc
    37141 gtctgttagt gccacccgcc tggcagaaca acccggatat ggacccggaa ctgcgtgcct
    37201 tctttgactt taactccatg catatggagc cgtgggatgg cccggcgggc atcgtgatgt
    37261 ccgacggtcg ttttgccgcc tgtaacctcg accgtaacgg tctgcgtccg gcgcgctacg
    37321 tcatcaccaa agataagctc atcacctgcg cctctgaagt cggtatctgg gattaccagc
    37381 ctgacgaagt ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtt atcgacaccc
    37441 gcagtgggca tattctgcac tcggcagaaa ccgatgacga tctgaaaagc cgccatccat
    37501 ataaagagtg gatggagaaa aacgtccgcc gactggtacc gtttgaagat ctgcccgatg
    37561 aagaagtggg tagccgcgaa ctggacgacg acacgcttgc cagctaccag aaacagttta
    37621 actacagcgc ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac ggtcaggaag
    37681 cggtcggttc gatgggcgat gataccccat tcgccgtgct ctccagtcag ccgcgcatta
    37741 tttacgacta cttccgccag cagtttgccc aggtgactaa cccgccaatc gacccgctgc
    37801 gtgaagcgca tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac gtcttttgcg
    37861 aagcagaggg ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc tactccgatt
    37921 tcaaacagct cacgacgatg aaagaggagc actaccgcgc agatacgctg gatatcacct
    37981 ttgacgtcac taaaaccacg ctcgaagcga cagtcaaaga gctgtgcgac aaagccgaaa
    38041 aaatggtacg tagcggcacc gtgctgctgg tgctctccga ccggaatatc gctaaagatc
    38101 gcctgccggt tccagccccg atggcggttg gcgcgatcca gacccgtctg gtcgatcaaa
    38161 gcctgcgttg cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat ccgcaccact
    38221 tcgccgtgtt gctgggcttc ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc
    38281 tgggccgcct ggtagacacc catgcgattg ccaaagatta tcgtaccgtg atgctcaact
    38341 accgtaacgg catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc atctccacca
    38401 tcgcctctta ccgctgctcg aaactgtttg aagcggtcgg tctacacgat gatgtagtgg
    38461 gcctgtgctt ccagggggcg gtcagccgca ttggtggggc aagctttgaa gacttccagc
    38521 aggatctgct gaatctgtcg aaacgtgcct ggctggcgcg taagcccatc agccagggcg
    38581 gcctgctgaa atacgtccac ggcggcgaat accacgccta caacccggac gtggtgcgca
    38641 cgctgcaaca agcggtacaa agcggcgagt acagcgacta tcaggaatac gcgaagctgg
    38701 ttaatgagcg tccggcaacc acgctgcgcg atctgctggc aattacgccg ggtgaaaacg
    38761 cggtcaacat tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttc gataccgctg
    38821 cgatgtctat cggcgcgtta agcccggaag cccacgaggc gctggcggaa gcgatgaaca
    38881 gcatcggcgg taactcaaac tccggtgaag gcggtgaaga tccggcgcgc tacggcacca
    38941 acaaagtgtc gcgcatcaag caggtggctt ccggtcgctt tggggttact ccggcgtatc
    39001 tggtcaatgc cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg ggcgaaggcg
    39061 gtcagttgcc gggtgataaa gtcactcctt acatcgccaa actgcgctat tcggtgcccg
    39121 gagtgacgct gatctccccg ccgccgcacc acgatatcta ctctatcgag gacttagcgc
    39181 agctcatttt cgacctcaag caggttaacc cgaaagcgat gatctccgtg aagctggttt
    39241 ccgaaccggg cgtaggcacc atcgcgactg gcgtggcaaa agcttatgcc gatttaatca
    39301 ccatcgcagg ctatgacggt ggcaccggcg caagcccgct gtcgtcagtg aaatacgcag
    39361 gttgcccgtg ggagctgggg cttgttgaaa cccagcaggc gctggttgcc aatggtctgc
    39421 gtcataagat ccgtttgcag gtcgatggcg gcctgaaaac cggtgttgat atcatcaaag
    39481 cggcgattct cggcgcagaa agcttcggct tcggcactgg cccaatggtc gcgctcggct
    39541 gtaaatatct gcgtatttgc cacctgaaca actgcgcaac gggtgtggca actcaggatg
    39601 acaaactgcg taagaatcac tatcacggcc tgccgttcaa ggtgacgaat tactttgagt
    39661 ttatcgcccg tgaaacccgc gagctgatgg cacagctggg ggtaacgcgg ctggtggatc
    39721 tgattggtcg caccgacctg ctgaaagagc tggacggttt caccgccaaa cagcagaaac
    39781 tggcgctgtc gaagctgctg gagaccgccg aaccgcatcc aggtaaggca ctctactgca
    39841 ccgaaaacaa cccgccgttt gataacggcc tgctgaacgc gcagttgctg caacaggcga
    39901 aaccgtttgt cgatgaacgc cagagcaaaa ccttctggtt cgatatccgc aataccgatc
    39961 gctccgtggg cgcgtcgctt tcaggctata tcgcccagac gcacggcgat caggggctgg
    40021 cagccgatcc tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc ggtgtatgga
    40081 acgcgggcgg cgtggaactg tacctgaccg gggatgccaa cgactatgtc ggtaaaggca
    40141 tggcgggcgg cttaatcgcc attcgtcctc cggttggttc cgccttccgc agccatgaag
    40201 caagtattat cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag
    40261 gccgcgcggg tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg gtagaaggca
    40321 ttggcgacaa cggctgtgaa tatatgacgg gcggtattgt ctgtattctg ggtaaaaccg
    40381 gcgttaactt cggtgcgggc atgaccggcg gcttcgctta cgttctcgat gaaagcggtg
    40441 atttccgcaa acgcgttaac ccggaactgg tcgaggtctt aagcgttgac gatctggcga
    40501 tccatgaaga gcatctgcgc ggtcttatca ccgagcatgt tcagcatact ggctctcagc
    40561 gcggtgaaga gattctggcg aactggtcaa ccttcgccac taaatttgcg ctggttaaac
    40621 cgaagtccag tgatgtaaaa gcactgctgg gtcatcgtag tcgtagcgca gcagagttgc
    40681 gcgtgcaggc gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc
    40741 tgcagcgcgt tgatccgcca aagaaaccgc tgaagatccg caaaattgag tttgttgaaa
    40801 tttacgagcc gttttccgaa ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg
    40861 gcaacccata ctgcgagtgg aaatgtccgg tacacaacta catcccgaac tggctgaagc
    40921 tcgccaacga ggggcgtatt tttgaagcgg cggaactgtc gcaccagacc aacaccctgc
    40981 cggaagtttg cggacgagtc tgcccgcaag accgtctgtg cgaaggttcc tgcactctga
    41041 acgatgagtt tggcgcggtg accatcggca acattgagcg ttatatcaat gataaagcgt
    41101 tcgaaatggg ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa aaagtggcga
    41161 ttatcggcgc aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt aacggcgtaa
    41221 aagccgttgt cttcgaccgt catccagaaa ttggcgggct gctgaccttc ggtattccgg
    41281 ccttcaagct ggaaaaagag gtaatgacgc gtcgccgtga aatcttcacc ggcatgggta
    41341 ttgaattcaa actcaatacc gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg
    41401 attacgatgc cgtcttcctt ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa
    41461 acgaagacgc cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac accaaacagt
    41521 taatgggctt tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg
    41581 tggtccttgg cggtggcgac actgcgatgg actgcgtgcg tacgtccgtg cgccagggag
    41641 cgaagcacgt tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg
    41701 aagtgaaaaa cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag ccactgggga
    41761 ttgaagtgaa cggtaacggc aaagtcagcg gcgtaaaaat ggtacgtacc gaaatgggcg
    41821 aaccggatgc caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa catatcgttc
    41881 cggcagatgc agtgatcatg gcgtttggtt tccgtccaca caacatggaa tggctggcaa
    41941 aacacagcgt cgagctggat tcacaaggcc gcatcatcgc cccggaaggc agcgacaacg
    42001 ccttccagac cagcaacccg aaaatctttg ctggcggcga tatcgtccgt ggttccgatc
    42061 tggtggtaac cgctattgcc gaaggtcgta aggcggcaga cggtattatg aactggctgg
    42121 aagtttaagc tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct
    42181 ttctttctgg aaagcgcctc gcataatctg aagaaaacca atgtaatacg ctcatctgga
    42241 ttgttaccgg tagatgcccg gcgtatagct gattccatta ttcaacgcag cgccatataa
    42301 agggacagat aatggaatcg ctctcagaga gaacctcaac aggctaccag caaatccacg
    42361 acggtattat tcacctggtc gatagcgccc ggacggaaac ggtacgtagc gttaacgcgc
    42421 tgatgaccgc gacatactgg gaaattggcc gacgaattgt cgaatttgaa caaggtggcg
    42481 aggcaagggc tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat ttaagtctaa
    42541 ggtataagcg tggtttctct gcaaaaaact tacgccaaat gaggcttttt tacctctttt
    42601 ttcaacatgt tgaaattcgc cagacagtgt ctggcgaatt aacaccattg ggaattccgc
    42661 agacaccatc tgcagaattc ccatcggcaa agatttggca gacgctgtct gccaaattga
    42721 gaggctgttt gatatttcca cagcttttgt attgcatctc ctattttcgg tttgtatatt
    42781 catgcaaata aacgaaggaa agttgccgtg atttacattg aacagcttga attaatccat
    42841 aaaagtggtg atgtgttgta tccagtaaaa ataaccagaa aatcatcagg aaaaactgcc
    42901 ttccaccttg ttccattcgg cttaaataag actcacgacc ttctagaggt ggaagatgct
    42961 tcagaagcaa ttcggctggt gattgatgaa cgtcattcca ttcgctgcag tacacttaca
    43021 gcaactatta cgaataaaaa gggcaagcga attaagcgga caggtatata cagtatcaag
    43081 ggtgttaaca taaaagaata caacgtcagg taatggcaca gagcggtccg tcagttcagg
    43141 tttggctctg tgccaaagat gggtcaggtc acacctgatc taataaattt aagccattaa
    43201 atcagcctta accttcacca ccaccttttt aatctcaccc ggctcgccaa caacgcatcc
    43261 cggcttatgc ggctctccta gcataaacac ggcgaacatt cccggtttca ggatgatggc
    43321 ttgctcgttt tcaatggtgc tgcaaagctg gtaatcatcc tcatggtgga actcttcaca
    43381 ctgacgcgca gtgcctgcca tgccaaacag aatccgttct tcaccgttta ataacagctg
    43441 gatatcaatg tattgctcgt gcagctccgc ttttttctca acgggcgatt gagtgttaaa
    43501 cgtcatgaca ttcataaaaa tattgtcgcc ctgtaattcg taacgacccg gcgctttttc
    43561 ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc
    43621 agacggtaat gaccgtactt cacccatcat cataattttt ctccctgggc caacagcgct
    43681 gccccaagta aacctgcatc atggcggtag tgcgccgcca gtaaatcaac atgaaatacc
    43741 gctggctcct gcgccagata cgtttccacc aacgccagat acccttctgc cagaccaacg
    43801 ctgccaccga ccaccacgca ctggcaatca gttgtggctt taatatcagc gatcagcctt
    43861 gccagcacat gtgcggagcg gtgaatcagt tgctgcgcct gctcgtcgcc ctgcccggcg
    43921 cgtgtgaaaa tagttctcgc atccgcgccc atgagttcac cttgcgctgc cgctgcaata
    43981 ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactgggccg
    44041 tgtggatcgg caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtaagcagt
    44101 ttaccgccgc tcactacacc gccgccgacg ccagtggaaa cggtgataaa caccatctcg
    44161 gttacatcgc cttccagcgc ctgatactct gcccatgctg cggcctgcgc gtcgttaatg
    44221 gcaatggtcg gcaaattggt aagttgttcc agcgttttga ctaacggaaa gtgtagcaat
    44281 ccaccgagat tatgcggatt cagcgccagc aaactgccgt cgcgaataat tccggttgaa
    44341 gcgatggcaa cccgctgcgc atgagcttgc aacggagaga ctaatgcgga taaggcatca
    44401 cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt cacgacgatc gcggatctgc
    44461 ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg
    44521 gtcatagcac cgcctttttc atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg
    44581 taatggcaga accgaccgtc accgcccacg cgccgtggcg catcgcatct gccgcctgag
    44641 caggcgtgtt gtaacgccct tcggcaatca cccgacatcc ggcttcgctc aacgttttca
    44701 ccagcgccag atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc
    44761 caataatttc ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg
    44821 tcatcgccag taaaccgtga tgatgtatac gtgccagcag cgtttcaaca ggcaccggac
    44881 gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga
    44941 catcttcaat ataggccgtg atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc
    45001 caataatcgg cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcgatgc
    45061 gaatggcaac cgcgcccgcc tgctctgccg ctaatgccat ggctgcgacg atttcgggtt
    45121 tatcgagcgg gctgtcagga atcggctggc aggagacaat caggccaccg ttagcagcga
    45181 ttttttgatc cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac
    45241 atagaccggg caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa
    45301 tggcacggca ccgctaaatg gtttgccgtc gatagcgtca tgagtacgca atgcttccgg
    45361 gcgcagccaa cgctgaacgc gggaaggcat atccagacca atcagcagga tcaccacaaa
    45421 tgtcaggctg aacgagagcg atgccagcgc agtacccaga tccagacgtt gagcgatcaa
    45481 cgcgccgatg attggggcca gtgcgccacc caatgcgcca acgttgtagg taaagcccag
    45541 gcccgctgca cgctggtcgg tatcgaaata accgccaatc agtttcggta agatcccggc
    45601 gatcccttgt ccaagcattt gctggaagaa cagtaacaga ccgagcaccc agacgtttgc
    45661 gccgccaatc gcaaataccg gaataatcag cagctgcgag gccagcaggc tacaaacgta
    45721 cgctttgcgg gttcccagcc agtcaccgag gaagcctcct acgcagcatc ccacggccgc
    45781 gccaaagcca ctaaagaaca gcacattggc gacagtatgc gggttataag ccagatcggt
    45841 tttcagatac gttggcagca gcgcctgaat cggccatgag tagaggaaag caaacaacac
    45901 gaccaccatc agcattacgc ccgttggcca gcgtttgcct gtactctgca ccataaagct
    45961 gataaagatt gcggcgcata acagcccaag aacagcgacg atcgcggcat tttgcaggtt
    46021 accggcgaag cagaaccaca gcgcagtagc cgccgccagt gtcattacga tattggcaat
    46081 gcgatgttca ccacggtaga gaatatccac cattgtgcgt actggtgctt tacctgcgtg
    46141 tttctctttc cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat
    46201 tggcaaaatg ccgataaaga acagcgcacg ccaaccccag accggaacca ccaggctata
    46261 gacctgagcg gcaacgacgg cccccacaga gaagcctgaa atcaaaaaac cactggcttt
    46321 gttacgcaga tgttttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc
    46381 cgccatcccc atgccgatga ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa
    46441 gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg
    46501 cccgtagcgg tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc
    46561 tgcggagatc agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt
    46621 gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc
    46681 ggaaaatgcg cgccattgtg cacggttgag atggcgatac cacgggatat tctgggttgt
    46741 agtactcatt gtgagtctcc cgcggtgggc gatgcccaca cgctttggta tgaaaattgt
    46801 agggtacaga tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaaacaactc
    46861 acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga tatttttcat
    46921 ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata taatgcagga
    46981 cagttttcag gccgcggaat acgccagttt tgatcagtaa atcaatgact ttgttgcact
    47041 cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgccttc acgatcccct
    47101 gatatcgcca gcccatgatg ttgtaagtac tgccgatacc accatcagcg cctgccagca
    47161 agccggaagc gaagatttcg tcgtaaccgt tatagagcac cagatcagga tgttcacgac
    47221 ggatctgttc catctgatag agatcgccag aagtctgttt cagcgcaccg acgccaggca
    47281 acgtaaccag ggtgttgatc tgatccaggg tcagttttac cccactcagg gctggaatgt
    47341 tgtacacaac catcggcaaa ccatccgctg aatcaataat tgcccgatag tgatcgcagt
    47401 gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca tcgaagccat
    47461 aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa ccgacgtggg
    47521 cgatgagttt aatcttacct ttcgcctctt cggcgacgat ttccagtacc tgttcacgct
    47581 cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa ccgtcgatgc
    47641 cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt gcttgttgtt
    47701 ggtcaaaagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc ataaatacct
    47761 ctgaagtgat acttgtctga taaacgatat acctttatac ctgttatacc agatcaatta
    47821 agcaacaccc catacagaaa gcttataatg cgatctgctt cactaaagtg gcattatttc
    47881 tttttgttgg tggtctgacc gaaagcgtgc caggtagcag agacgctgtt gagatgcgat
    47941 tgcaacgcac gatcggcttc gtcaggatca tgacggcgga tcgcatcaac gatcgcaata
    48001 tgctgttgat agctaacgtt gttatgctcg tgcagagcct gatcagccac ggttggacgt
    48061 gcggcgatca gccagtcgag cagggcaacg tggatcgcca tgaagattgg gttaccaggg
    48121 atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc
    48181 agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc atcggtggca
    48241 tgttcagccg catagcgcac cagactggat tcaaagaaca gacgtaattg ttcgaaatgg
    48301 gcaatcccac cgggatgaga aaggaaatct ttcgccatgc cggaaaactc accgatgata
    48361 gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg caccagacct
    48421 ttgcgtttta acgctgccag cgcttcgcgc acagaaggac gcccgacgtt aaagaacgcc
    48481 atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg gcggatcatc
    48541 tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg gcggctacgc
    48601 aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc gttcataagg
    48661 cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac aggattgaaa
    48721 gtaggggaaa atggcaggtt tttctctttg tgcctcatca ttaccataat taacggaata
    48781 attaactatt gcgaaaaatt aatgtaacgc agataaacac atcccgattg aattattcat
    48841 aagactattc acaagcatta tgaatattat gaatgtgttc ttacaaaata atcataagcg
    48901 catatttttt aatgaaaaat cacatcacct acaattaaaa acacgacatc cgcaccataa
    48961 atagacttgc aaaaaatata acatcattgt tttcaatctg ccgtttatgg gattgaccgt
    49021 tttcttttga cacggagttc aacaatgttc ggcataatta tatctgtcat cgtattaatt
    49081 acgatgggct atttgatcct gaaaaactac aaacctcagg tggtgctggc tgccgcaggt
    49141 gtcttcctga tgatgtgcgg agtctggtta gggttcggtg gtgtactcga tcccgccaaa
    49201 agcagcggct acttgatcgt cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc
    49261 attgccggat tggggctgtc gattatggcg gtgggcggtt atgcccgcta catggagcgc
    49321 ataggagcca gtcgcgcgat ggtgagcttg ctaagccgcc cgttaaaact cattcgctcg
    49381 ccgtatatta ttctgtcggc aacttacgtc atcggccaaa tcatggcgca gtttattacc
    49441 agcgcctccg gtctgggtat gttgctgatg gtcaccttat ttccgacgct ggtgagtctg
    49501 ggagtaagtc gcctctctgc ggtggcggtt atcgcaacca cgatgtccat tgagtggggg
    49561 attctggaaa cgaactccat ttttgcagcc caggtcgcgg gaatgaaaat tgccacatac
    49621 ttcttccact accagcttcc ggtcgcctct tgcgtcatta tctcggtggc gatctcccac
    49681 tttttcgtgc aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga acaggcagag
    49741 caaaaaactc tcgataatgt cccgccgctc tattacgcca ttttacctgt gatgccgtta
    49801 atcctgatgc tcggctcgct gttcctcgcc cacgtcgggc tgatgaagtc agaactgcat
    49861 ctggtggtgg tgatgttact aagtttgacg gtgacgatgt ttgttgagtt cttccgcaag
    49921 cataacttgc gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat gggtacgcag
    49981 tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg
    50041 attggcactg ttgatgcggt tatcaggggt gcggagcatt ctggtctggg cggtattggc
    50101 gtgatgatta ttatggcgct agtcattgcc atttgtgcca ttgtgatggg ctctggcaat
    50161 gcgccgttta tgtcatttgc cagtcttatt ccgaatatcg cagccggact acatgtacca
    50221 gcggttgtaa tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg
    50281 attactgcgg tggtgaagcg gacagcgatc cccatggcag tcggtttcgt ggtgaatatg
    50341 attgccacaa tcacgctatt ttattaagtc attaaaaaga caaaacaggc cgcctgggcc
    50401 tgttttgtat tacttcacaa cacgtaatgc cggtcgacca ccgcgtggtg gctgcggagg
    50461 ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
    50521 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat cttcatcgta
    50581 ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac
    50641 ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac
    50701 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
    50761 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag
    50821 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
    50881 acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt
    50941 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
    51001 ttcagctctt tcgcacccgg gccgctgagc tcgataccca gttgcggcag acgccacagc
    51061 agcggagcaa gatagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga
    51121 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
    51181 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
    51241 tgcatgtaca gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
    51301 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
    51361 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
    51421 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
    51481 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
    51541 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
    51601 ggccagtcag aagttgtgtt acccaataag gaacgactct ctttgttcga aaatcaaaca
    51661 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
    51721 gaccattgtg gtgagtggat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
    51781 agttgactta gacccttatt ggcgatgtgg tttttgtttt gcctgtcttt cagacggcag
    51841 caaaaagcaa cttcccattt tttacgctga ttcagaattt agccataaaa aaacccgccg
    51901 aagcgggttt tttcgtaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
    51961 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
    52021 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
    52081 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
    52141 ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac
    52201 catgcgggca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
    52261 accgttgccc ggtttgatga aaacgcgagc tgcggaattt ttgcggcgac cagtgccgta
    52321 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
    52381 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
    52441 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
    52501 agcaatcatc tcttcaaagc tcacttgttt gataccaccg atgtggccgg tgtggtgata
    52561 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
    52621 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgtaggcg
    52681 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
    52741 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
    52801 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
    52861 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
    52921 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
    52981 tttgtgattt gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt
    53041 tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg atcaccctga
    53101 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
    53161 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
    53221 acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg
    53281 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
    53341 gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt cccccccgcc
    53401 agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg cgaaagccac
    53461 agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca
    53521 tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caagccattt
    53581 cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
    53641 aaggctttca tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg
    53701 tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc cagcggatcg
    53761 gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc
    53821 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
    53881 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
    53941 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
    54001 gtgctattga tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg
    54061 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
    54121 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
    54181 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
    54241 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
    54301 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
    54361 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
    54421 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
    54481 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
    54541 tatcagcaca tggcgaaaag ttccagcagc ctgctgccgg aactgtctgc tgaagcaaac
    54601 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
    54661 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
    54721 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
    54781 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
    54841 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaacccca gctgttgagt
    54901 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt
    54961 ccaggccagg ttgccgatca ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg
    55021 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
    55081 gaagaattca aaaagttttt tggtgatgat ttaccggagc aacctgcaca acccttcgaa
    55141 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
    55201 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
    55261 aaactgattg gtagcgatga ccagagcgat atcgccctgt tacaaattca aaacccgagc
    55321 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgta
    55381 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
    55441 ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
    55501 aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac
    55561 actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg gatttgccat ccccagtaat
    55621 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
    55681 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
    55741 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa
    55801 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
    55861 tctcgtatcg cgaccaccga gccgggcaca aaagtgaaac ttggcctgct gcgtaacggc
    55921 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
    55981 atgatcgcgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
    56041 aaaggtatta agatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
    56101 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
    56161 aaagtgctgg cggcaaaatc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
    56221 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg
    56281 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
    56341 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
    56401 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
    56461 ctgccagcta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
    56521 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
    56581 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
    56641 tcatcgtcgc cttacaggat ggtcgtgtat ttgaagcatt gctggtggga tctgactctc
    56701 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccaatca
    56761 atgcacgtcg cgtaccgcat attggcgacg tggtactggc gatcggtaac ccgtacaacc
    56821 tcgggcagac cattacccag gggattatta gtgccacggg tcgaattggt ctgaacccga
    56881 ccgggcggca aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg
    56941 cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta
    57001 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
    57061 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggc attggcggac
    57121 gtgagatcgc accgctgcac gcacagggcg gtggtataga tcaactgcaa gggatcgtgg
    57181 ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga
    57241 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcgg
    57301 aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
    57361 tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa
    57421 ccggagtctg tgc
//
LOCUS       DAJVYM010000008        57265 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVYM010000008 DAJVYM010000000
VERSION     DAJVYM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.008"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1529)
                     /gene="hyfB"
                     /locus_tag="OQS60_000460"
     CDS             complement(<1..1529)
                     /gene="hyfB"
                     /locus_tag="OQS60_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCS2386319.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TP"
     gene            complement(1529..2146)
                     /gene="hyfA"
                     /locus_tag="OQS60_000461"
     CDS             complement(1529..2146)
                     /gene="hyfA"
                     /locus_tag="OQS60_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS2386320.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(2399..2869)
                     /gene="bcp"
                     /locus_tag="OQS60_000462"
     CDS             complement(2399..2869)
                     /gene="bcp"
                     /locus_tag="OQS60_000462"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS2386321.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(2869..3441)
                     /locus_tag="OQS60_000463"
     CDS             complement(2869..3441)
                     /locus_tag="OQS60_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS2386322.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            3587..4465
                     /gene="dapA"
                     /locus_tag="OQS60_000464"
     CDS             3587..4465
                     /gene="dapA"
                     /locus_tag="OQS60_000464"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS2386323.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            4482..5516
                     /gene="bamC"
                     /locus_tag="OQS60_000465"
     CDS             4482..5516
                     /gene="bamC"
                     /locus_tag="OQS60_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS2386324.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            5729..6442
                     /gene="purC"
                     /locus_tag="OQS60_000466"
     CDS             5729..6442
                     /gene="purC"
                     /locus_tag="OQS60_000466"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS2386325.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            6610..7458
                     /locus_tag="OQS60_000467"
     CDS             6610..7458
                     /locus_tag="OQS60_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS2386326.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            7473..9488
                     /gene="tmcA"
                     /locus_tag="OQS60_000468"
     CDS             7473..9488
                     /gene="tmcA"
                     /locus_tag="OQS60_000468"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS2386327.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            9562..10260
                     /gene="ypfH"
                     /locus_tag="OQS60_000469"
     CDS             9562..10260
                     /gene="ypfH"
                     /locus_tag="OQS60_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS2386328.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(10370..10570)
                     /locus_tag="OQS60_000470"
     CDS             complement(10370..10570)
                     /locus_tag="OQS60_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS2386329.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(10598..11725)
                     /gene="dapE"
                     /locus_tag="OQS60_000471"
     CDS             complement(10598..11725)
                     /gene="dapE"
                     /locus_tag="OQS60_000471"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS2386330.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(11729..12085)
                     /locus_tag="OQS60_000472"
     CDS             complement(11729..12085)
                     /locus_tag="OQS60_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS2386331.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            12467..12526
                     /gene="ypfM"
                     /locus_tag="OQS60_000473"
     CDS             12467..12526
                     /gene="ypfM"
                     /locus_tag="OQS60_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS2386332.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(12625..15738)
                     /gene="acrD"
                     /locus_tag="OQS60_000474"
     CDS             complement(12625..15738)
                     /gene="acrD"
                     /locus_tag="OQS60_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS2386333.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15902..17602)
                     /gene="narQ"
                     /locus_tag="OQS60_000475"
     CDS             complement(15902..17602)
                     /gene="narQ"
                     /locus_tag="OQS60_000475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS2386334.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17808..19787
                     /gene="aegA"
                     /locus_tag="OQS60_000476"
     CDS             17808..19787
                     /gene="aegA"
                     /locus_tag="OQS60_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS2386335.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            19855..20430
                     /gene="nudK"
                     /locus_tag="OQS60_000477"
     CDS             19855..20430
                     /gene="nudK"
                     /locus_tag="OQS60_000477"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS2386336.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            20556..21599
                     /locus_tag="OQS60_000478"
     CDS             20556..21599
                     /locus_tag="OQS60_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS2386337.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(21695..23698)
                     /gene="tkt"
                     /locus_tag="OQS60_000479"
     CDS             complement(21695..23698)
                     /gene="tkt"
                     /locus_tag="OQS60_000479"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS2386338.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(23718..24668)
                     /gene="tal"
                     /locus_tag="OQS60_000480"
     CDS             complement(23718..24668)
                     /gene="tal"
                     /locus_tag="OQS60_000480"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS2386339.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            24957..27236
                     /gene="maeB"
                     /locus_tag="OQS60_000481"
     CDS             24957..27236
                     /gene="maeB"
                     /locus_tag="OQS60_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS2386340.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            27529..27864
                     /gene="eutS"
                     /locus_tag="OQS60_000482"
     CDS             27529..27864
                     /gene="eutS"
                     /locus_tag="OQS60_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS2386341.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            27877..28356
                     /gene="eutP"
                     /locus_tag="OQS60_000483"
     CDS             27877..28356
                     /gene="eutP"
                     /locus_tag="OQS60_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS2386342.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            28331..29032
                     /gene="eutQ"
                     /locus_tag="OQS60_000484"
     CDS             28331..29032
                     /gene="eutQ"
                     /locus_tag="OQS60_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCS2386343.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            29029..29832
                     /gene="eutT"
                     /locus_tag="OQS60_000485"
     CDS             29029..29832
                     /gene="eutT"
                     /locus_tag="OQS60_000485"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCS2386344.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            29829..30845
                     /gene="pta"
                     /locus_tag="OQS60_000486"
     CDS             29829..30845
                     /gene="pta"
                     /locus_tag="OQS60_000486"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS2386345.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            30884..31177
                     /gene="eutM"
                     /locus_tag="OQS60_000487"
     CDS             30884..31177
                     /gene="eutM"
                     /locus_tag="OQS60_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCS2386346.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            31284..31571
                     /gene="eutN"
                     /locus_tag="OQS60_000488"
     CDS             31284..31571
                     /gene="eutN"
                     /locus_tag="OQS60_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCS2386347.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            31583..32986
                     /locus_tag="OQS60_000489"
     CDS             31583..32986
                     /locus_tag="OQS60_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCS2386348.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            32997..33833
                     /gene="eutJ"
                     /locus_tag="OQS60_000490"
     CDS             32997..33833
                     /gene="eutJ"
                     /locus_tag="OQS60_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCS2386349.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            33823..35010
                     /gene="eutG"
                     /locus_tag="OQS60_000491"
     CDS             33823..35010
                     /gene="eutG"
                     /locus_tag="OQS60_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCS2386350.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            35227..36453
                     /gene="eutH"
                     /locus_tag="OQS60_000492"
     CDS             35227..36453
                     /gene="eutH"
                     /locus_tag="OQS60_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCS2386351.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            36450..37853
                     /gene="eutA"
                     /locus_tag="OQS60_000493"
     CDS             36450..37853
                     /gene="eutA"
                     /locus_tag="OQS60_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS2386352.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            37865..39226
                     /gene="eutB"
                     /locus_tag="OQS60_000494"
                     /pseudo
     CDS             37865..39226
                     /gene="eutB"
                     /locus_tag="OQS60_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            39247..40134
                     /gene="eutC"
                     /locus_tag="OQS60_000495"
     CDS             39247..40134
                     /gene="eutC"
                     /locus_tag="OQS60_000495"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCS2386353.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            40144..40803
                     /gene="eutL"
                     /locus_tag="OQS60_000496"
     CDS             40144..40803
                     /gene="eutL"
                     /locus_tag="OQS60_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS2386354.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            40816..41316
                     /gene="eutK"
                     /locus_tag="OQS60_000497"
     CDS             40816..41316
                     /gene="eutK"
                     /locus_tag="OQS60_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS2386355.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            41362..42414
                     /gene="eutR"
                     /locus_tag="OQS60_000498"
     CDS             41362..42414
                     /gene="eutR"
                     /locus_tag="OQS60_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS2386356.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(42420..43319)
                     /gene="hemF"
                     /locus_tag="OQS60_000499"
     CDS             complement(42420..43319)
                     /gene="hemF"
                     /locus_tag="OQS60_000499"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS2386357.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(43323..44192)
                     /gene="amiA"
                     /locus_tag="OQS60_000500"
     CDS             complement(43323..44192)
                     /gene="amiA"
                     /locus_tag="OQS60_000500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS2386358.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            44406..44831
                     /locus_tag="OQS60_000501"
     CDS             44406..44831
                     /locus_tag="OQS60_000501"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS2386359.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            44818..45267
                     /locus_tag="OQS60_000502"
     CDS             44818..45267
                     /locus_tag="OQS60_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS2386360.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            45328..45903
                     /locus_tag="OQS60_000503"
     CDS             45328..45903
                     /locus_tag="OQS60_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS2386361.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            45999..46898
                     /gene="yfeX"
                     /locus_tag="OQS60_000504"
     CDS             45999..46898
                     /gene="yfeX"
                     /locus_tag="OQS60_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS2386362.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(46956..48260)
                     /gene="pbp4b"
                     /locus_tag="OQS60_000505"
     CDS             complement(46956..48260)
                     /gene="pbp4b"
                     /locus_tag="OQS60_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCS2386363.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(48265..48438)
                     /locus_tag="OQS60_000506"
     CDS             complement(48265..48438)
                     /locus_tag="OQS60_000506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCS2386364.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(48451..49689)
                     /gene="murP"
                     /locus_tag="OQS60_000507"
                     /pseudo
     CDS             complement(48451..49689)
                     /gene="murP"
                     /locus_tag="OQS60_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(49693..50589)
                     /gene="murQ"
                     /locus_tag="OQS60_000508"
     CDS             complement(49693..50589)
                     /gene="murQ"
                     /locus_tag="OQS60_000508"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS2386365.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            50753..51610
                     /gene="murR"
                     /locus_tag="OQS60_000509"
     CDS             50753..51610
                     /gene="murR"
                     /locus_tag="OQS60_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS2386366.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            51739..52530
                     /gene="ucpA"
                     /locus_tag="OQS60_000510"
     CDS             51739..52530
                     /gene="ucpA"
                     /locus_tag="OQS60_000510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS2386367.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            52701..53717
                     /gene="cysP"
                     /locus_tag="OQS60_000511"
     CDS             52701..53717
                     /gene="cysP"
                     /locus_tag="OQS60_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS2386368.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            53717..54550
                     /gene="cysT"
                     /locus_tag="OQS60_000512"
     CDS             53717..54550
                     /gene="cysT"
                     /locus_tag="OQS60_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS2386369.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            54550..55425
                     /gene="cysW"
                     /locus_tag="OQS60_000513"
     CDS             54550..55425
                     /gene="cysW"
                     /locus_tag="OQS60_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS2386370.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            55415..56512
                     /gene="cysA"
                     /locus_tag="OQS60_000514"
     CDS             55415..56512
                     /gene="cysA"
                     /locus_tag="OQS60_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS2386371.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            56646..>57265
                     /gene="cysM"
                     /locus_tag="OQS60_000515"
     CDS             56646..>57265
                     /gene="cysM"
                     /locus_tag="OQS60_000515"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS2386372.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIP"
BASE COUNT        13371 a        14827 c        15957 g        13110 t
ORIGIN      
        1 ggggtattgg taaacgcatg ggcaatgtgc attatcttcg gtgccagcca actggcacta
       61 agcgcaatta atacgcagag tgccgcgagc agtagcatgg cgacgatcat cgtatttggc
      121 acttcctgtg cctcttcagc gtgtgtgctg cgcggcgcac cacagaaagt aataccgtac
      181 attttgacga agcacattac tgccagccca ccagtgactg ccagcattac catagcaata
      241 ggacccgcaa gttgtagcgc tacggcttcc acacgactta gtgagaatag cgactgccag
      301 gtgtaccatt cgctgataaa accattcagc ggaggaatgg ctgatatcgc caggcaacca
      361 atcaggcatg ctgcggctgt ccacggcatc cgtttcgcca gtgcccccat tttttccatg
      421 tcgtgggtat gcaaacgcga aataatcgca cccgcgccga gaaatagcag ccctttgaaa
      481 agcgcatggt tgagcagatg aaacagtgcg ccgagcagtc caaccacggt gagcagcggg
      541 tcgtgcagtg acagaccgac catcgccaca ccgaccgcca gcaaaataat gccgacgttt
      601 tcgacggtac tccaggccag cagccgtttg atatcctgtt ccgccagtgc atacagcacg
      661 cccaggagcg cggagattgc gccgatcgcc atcaccagaa tgccccacca cagcggcaaa
      721 cccgtttgcg ccagcagatc catcgccact ttcaggatgc cgaaaatacc gattttgacc
      781 attacgccag acatcaacgc tgaagcgtgc gatggtgcgg cagggtgagc gcgcggcaac
      841 cagctgtgca acggcatcat cccggctttc gcgccaaaac cgaaaaaggc cagcaggaac
      901 accgccgacg ccagccccgg agaaagtgaa agcgtgcgga aactggcaaa atcgaggctg
      961 ccgctttcgc gccccatcag caagaaggcg atcattatca gcaccgatcc ggcgtgggcg
     1021 ataaaaaagt agagcatgcc agcgttgatc gacgttttat cctgcctggc aatgaccaga
     1081 aaccaggaag acagcgacat catttcaaac agcacgatga accaaaaagc gttgtccatc
     1141 accagcaggg caaccatcga tgcgatgaaa atattcataa agaagcccat cgccgccgcg
     1201 cctttgccct cgtattcgcg catataagtc aatgaataga gagaacaaac caccaccagc
     1261 aaggagataa ccagcaccat aaatgccgag aggctatcca ttcgcagggt taaatcggca
     1321 aattcaaaag gcgtggcaaa acgggcgact aaagttacgc ctgcatgtaa ttgcgtaatt
     1381 ccgctgatga tgccaatcac gccgcccacc agcgatgtga tgccggaaag cttaatagcc
     1441 agtctgtcca gaccgagtaa aaacagcgaa gccagactgg caaacagata gaggatcagc
     1501 gaccaggtta ataattgcag ggcatccatt agcgttgctc ctgagtgagg gaaaggggat
     1561 cctccccgcc attggcaaac cagcttgcgg caaggcgctg tttttctttc atctggcgtt
     1621 gcaggctatc gtcggtgatc agccgtaacg cctgattcgg gcaagcgcga acgcaggccg
     1681 gaccttctgg caagaaatca cacaggtcgc atttcaccgc gacggtctgg acacccggtt
     1741 cccagcgcag caacgcatgt tggggcggca cgttttcttc gccgtctttg agtgagccct
     1801 cagcctgaaa aacatattgc gcaggggcat tcaccggacg gcttcctgaa gcgctgattg
     1861 cgccaaatgg gcagaccacg gcgcaaagct tgcagccaat acagaggctt tcgttgagtt
     1921 ggatcgcatc atccctctga gagatggcat tgaccgggca gacctgcagg caaggggctt
     1981 cctcacagtg atgacacacg acaggggcag tgagtgttga cgtcttcgcc agggccaggc
     2041 gcgggtgttg ctgtaaacct tgcgttttat gcacgtccga acaggcagcg agacaggtat
     2101 tacatcctgt acaccacagt ggttcggcca ccacaaagcg gttcatgtcg gctccttgaa
     2161 aatgatatga aacattgcat ttcatattgc ataaaggatg ctattctgat taactcaata
     2221 tattcaataa gttatgtatt aactggattc tgaacggtac aaaaatgatg gtcattaacg
     2281 ttcagcaaaa aatggattcg acacccgtgt cgcaggggaa agcgagaatg tgatcttgat
     2341 cacgagaagt gaggtatgct ggccgcaagc gcagccagca cggaatggag caaagtaatc
     2401 aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg attgctggtt ttgaaatcgt
     2461 caaagacatg ttcgattttg ccatcagcgt caatcaggaa gctgatgcga tgaatgccat
     2521 cgtaggtttt gcccatgaag gacttttcac cccagacgcc gaattgttcg cacacctggt
     2581 ggtcctcatc agacaggagc gtaaagttaa gcagctcttt ttccgcaaaa cgggagagtt
     2641 tttcgggttt atcggtgctg atacccagca catcaacgcc cgcttttttc aactcatcca
     2701 tgttatcgcg taagccgcag gcctgtacgg tacagccggg ggtcatggct ttcgggtaga
     2761 aataaaccag aacacgctgt ccctggaagt cggtcaaatt aacttgttct ccgtcttgat
     2821 ccggcaagct aaatttcggt gcgatatcac cggctttcag tggattcatt acttaactcc
     2881 atcctgttca tcatgttggg aataattgac gacgttaata ctgccttgtg cattgagttc
     2941 tgtacatagg gctttgaacg cttgctcaat atttgctgcg tccgcagatg cggggctgtg
     3001 ggcggttatc tgaatatgca actgcgcagc ccgttcattt tcagcaggtt gcgtgcgcga
     3061 caccagctcc gcaatgttca tatgatgcgc gtcgaaaagt gctgtgaagc gttcaattaa
     3121 atgcggggag tctgccacat cgacctgaac ccagacagat gctggcattg gcggacgcgg
     3181 acgcgccgtc gtgcgcttca tcacgattaa aagatccagc tcggcacctt tcaacggtaa
     3241 cgttgattca atcagagtaa tggcattcca tgaaccggaa agcagcatga taaacgtgaa
     3301 ctcttctccc agcatcgcca ggcgactgtc ttcaatatta cagccgcaac tactgacatg
     3361 acgggtgatg gtgttcacaa ttccagggcg atcggcaccc aatgcagtga tcaccagata
     3421 atgttgcgat gacagtgtca aactggttat tcctttaagg ggtgagttgt tcttaaggaa
     3481 agcataaaaa aaacatgcat acaacaatca gaacggttct gtctgcttgc ttttaatgcc
     3541 ataccaaacg taccattgag acacttgttt gcacagagga tggcccatgt tcacgggaag
     3601 tattgtcgcg attgttactc cgatggatga aaaaggtaat gtctgtcggg ctagcttgaa
     3661 aaaactgatt gattatcatg tcgccagcgg tacttcggcg atcgtttctg ttggcaccac
     3721 tggcgagtcc gctaccttaa atcatgacga acatgctgat gtggtgatga tgacgctgga
     3781 gctggctgat gggcgcattc cggtaattgc cgggaccggc gctaacgcta ctgcggaagc
     3841 cattagcctg acgcagcgct tcaatgacag tggtatcgtc ggctgcctga cggtaacccc
     3901 ttactacaat cgtccgtcgc aagaaggttt gtatcagcat ttcaaagcca tcgctgagca
     3961 tactgacctg ccgcaaattc tgtataatgt gccgtcccgt actggctgcg atctgctccc
     4021 ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc ggaatcaaag aggcaacagg
     4081 gaacttaacg cgtgtaaacc agatcaaaga gctggtttca gatgattttg ttctgctgag
     4141 cggcgatgat gcgagcgcgc tggacttcat gcaattaggc ggtcatgggg ttatttccgt
     4201 tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc aaactggcag cagaagggca
     4261 ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca ttacacaaca aactatttgt
     4321 cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa ctgggtcttg tggcgaccga
     4381 tacgctgcgc ctgccaatga caccaatcac cgacagtggt cgtgagacgg tcagagcggc
     4441 gcttaagcat gccggtttgc tgtaaagttt agggagattt gatggcttac tctgttcaaa
     4501 agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt attactcgct gcctgtagtt
     4561 ctgactcacg ctataagcgt caggtcagtg gtgatgaagc ctacctggaa gcggcaccgc
     4621 ttgcggagct tcatgccccg gctggaatga ttttgccggt gacctccggt gattatgcaa
     4681 tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct ggacattcgt ccaccagccc
     4741 agccgctggc actggtttct ggcgcgcgta cccagttcac gggcgatacc gcttcattgc
     4801 tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt ggttagcgtg ctgcaggcga
     4861 aaaactacac catcacccaa cgtgatgatg ctggtcagac actgaccacc gattgggtac
     4921 aatggaaccg tctggacgaa gacgagcagt atcgtggtcg ttatcaaatc tctgttaagc
     4981 cgcagggtta tcagcaggcg gttacggtta aactgttgaa cctggaacag gcgggcaaac
     5041 cggttgcaga cgcggcttcc atgcagcgtt acagcacgga gatgatgaac gttatttccg
     5101 ccggtctgga taaatctgcc actgacgccg cgaacgctgc gcaaaatcgt gcctccacca
     5161 ctatggacgt acaaagtgca gctgatgaca ccggtttacc aatgctggtc gtacgcgggc
     5221 cgttcaatgt ggtttggcaa cgtctgccag cggcgctgga aaaagtgggc atgaaagtga
     5281 ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta taagccgctg tctgacagcg
     5341 actggcagga actgggcgcg agcgatccag gcctggcatc cggtgactat aaactgcagg
     5401 ttggcgattt agataaccgc agcagcctac agttcatcga tccgaaaggt catactctga
     5461 ctcagagtca gaacgacgcg ctggtagctg tcttccaggc tgcgtttagc aagtaaaaat
     5521 acagggctgg aatcatccgg cccttttttc tgatatgata cgcaaacgtg tgcgtcggca
     5581 ggaaaacgcg attttagcgg taattcgcac gaaatttgtt tgtcggacgt agttcggata
     5641 aggcgttcac gccgcatccg acaaaacatc cggcacacca gacagcaaaa gattttaaaa
     5701 cgttaattca cacccaggag tgataaagat gcaaaagcaa gctgagttgt atcgtggtaa
     5761 agcgaaaacc gtatacagca cggaaaaccc ggacctgttg gtgctcgaat tccgcaatga
     5821 tacgtcagca ggggatggcg cgcgcattga gcagtttgat cgcaaaggta tgttgaacaa
     5881 caagttcaac tacttcatta tgagcaaact ggctgaagcg ggtatcccga ctcaaatgga
     5941 gcgtctgctc tccgataccg aatgtctggt gaaaaagctg gatatggtgc cggttgagtg
     6001 tgtcgtgcgt aaccgtgctg cgggctctct ggtgaaacgt cttggaatcg aagaaggtat
     6061 tgagctgaac ccgccgctgt tcgatctgtt cctgaaaaac gatgccatgc acgatccgat
     6121 ggtcaacgaa tcttactgcg aaacctttgg ctgggtgagc aaagagaacc tggcgcgtat
     6181 gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa ctgttcgatg atgctggtct
     6241 gattctggtc gacttcaagc tggagtttgg tctgtacaaa ggtgaagtgg tactgggcga
     6301 tgagttctcc ccggacggca gccgcctgtg ggacaaagaa acactggaga aaatggacaa
     6361 agaccgtttc cgccagagcc tcggtggcct gatcgaagcc tatgaagccg tcgcccgccg
     6421 cctgggtgta cagctggact gatttttctg ttcatcatct tgccgtgcag ctggcacggc
     6481 aagacaaccg ctctcggaat atatgcattg aacgctcatt cctccgccat ttcatcccgc
     6541 tttcctgtgg taatcttcct ctgaaccacc tacgatcatt tctataatga atatattgtt
     6601 gaggtatcta tgcgttggca agggcgacgt gaaagtgaca atgtcgaaga caggcgtaac
     6661 agctccggcg gtccgtcaat gggcggtccc ggttttcgcc tgccaagcgg taaaggcggg
     6721 ctgattttac tgatagtcgt gctggttgca ggctactatg gtgttgattt aaccgggttg
     6781 atgaccggac agccggtttc ccaacagcaa tcaacacggt caattagccc aaatgaagac
     6841 gaagccgcaa aattcacctc ggtgattctg gcaaccacgg aagacacctg gggacagcaa
     6901 ttcgagaaga tgggtaaaac ctatcagcaa ccgaagctgg tcatgtatcg tggaatgacg
     6961 cgtaccggct gcggggcggg ccagtccatt atgggaccgt tctattgccc ggcggatggc
     7021 acggtttata tcgatctctc cttctatgat gacatgaaag acaaactcgg cgcggatggc
     7081 gattttgccc aggggtacgt tatcgcccat gaagtcggtc atcatgtgca gaaattgtta
     7141 ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga agtaaaccgc
     7201 ttatctgtgc gtatggaact ccaggccgac tgttttgccg gtgtctgggg gcataacatg
     7261 cagcagcaag gcgatctgga agaggcgctg aacgcggcgc aggccatcgg cgatgaccgt
     7321 ttacaacagc aaagtcaggg gcgagtagta ccagacagtt tcactcatgg tacttctcag
     7381 caacgctaca gctggtttaa acgtggtttc gacagcggcg atccggcaca atgcaatact
     7441 tttggtaaaa gcatttaact tccggggcaa ggatggctga actgactgcg cttcacacat
     7501 taacagcgca aatgaaacgt gaagggatcc gccgcttgct ggtgttgagc ggggaagagg
     7561 gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt acctggcgac tggctgtgga
     7621 tttcgccgca gccagatgct gaaaaccact gttctccctc ggcgctacaa actttacttg
     7681 ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg ctttgatgcc gctgcctttg
     7741 cggcacttag cggaacgttg aaagcgggaa gctggctggt tttgttactc cctgtatggg
     7801 aagagtggga aaaccaacct gatgccgact cgctgcgctg gagtgattgc cctgacccta
     7861 ttgcgacgcc gcattttgtc cagcatctca aacgcgtact tacggcggat aacgacgcta
     7921 ttctctggcg gcaaaaccag ccgttctcgt tggcgcattt taccccccgt actgactggc
     7981 accccgcgac cggcgcacca caaccagaac aacagcaact cttacagcag ctactgacca
     8041 tgccgccggg cgtggcagcg gtaacggctg cgcgtgggcg cggtaagtcc gcgctggcag
     8101 ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac cgcgcccgca aaagcggcaa
     8161 cggatgtact ggcacaattt gcgggcgaga agtttcgctt tattgcgccg gatgccttgt
     8221 tagccagcga tgagcaagcc gactggctgg tggtcgatga agccgcagcc atacctgcgc
     8281 cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt gttaaccact acggtgcagg
     8341 gctacgaagg taccggacgt ggttttttgc tgaaattttg cgctcgcttt ccgcatttac
     8401 accgttttga actgcaacag ccgatccgct gggcacaggg atgcccgctg gaaaaaatgg
     8461 ttagtgaggc actggttttt aacgatgaaa acttcaccca tacaccacaa ggcaatatcg
     8521 tcatttccgc atttgaacaa acgttatggc gaagcgatcc agaaacgccg ttaaaggttt
     8581 atcagttatt gtctggtgcg cactaccgga cctcgccgct ggatttacgc cggatgatgg
     8641 atgcaccagg gcaacatttt ttacaggcgg ctggcgaaaa cgagattgcc ggggcgttgt
     8701 ggctggtgga tgagggcgga ttatctcaag aactcagtca ggcggtatgg gcaggttttc
     8761 gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc gcacggcagc aatccactgg
     8821 cggcgacatt gcgcggacgg cgggtcagcc ggattgcagt ccatccggcg cgtcagcgcg
     8881 aaggcgttgg gcaacagctc attgccagcg ctttgcaata taggcctggc ctcgactatc
     8941 tttcggtgag ttttggttac accggggagt tatggcgttt ctggcaacgc tgcggttttg
     9001 tgctggtgcg aatgggtaat catcgtgaag ccagcagcgg ttgctatacg gcgatggcac
     9061 tgttaccgat gagtgatgcg ggtaaacagc tggctgaacg tgagcattac cgtttacgtc
     9121 gcgatgcgca agctctcgcg cagtggaatg gcgaaacgct tcctgttgat ccactaaaca
     9181 atgccgtcct ttctgacgac gactggcttg aactggccgg ttttgctttc gctcatcgtc
     9241 cgctattaac gtcgttaggt tgcttattgc gtctgctaca aaccagtgaa ctggcattac
     9301 cggcgctgcg tgggcgttta cagaaaaaca ccagtgacgc gcagttatgt accacactta
     9361 aactttcagg ccgcaagatg ttactggttc gccagcggga agaggccgcg caggcgctgt
     9421 tcgcacttaa tgaggttcgc actgagcgtc tgcgcgatcg cataacgcaa tggcaatttt
     9481 ttcactgact ccttcaattt tctcccatgg tcattgtgag tcagcggcgt aaaattaatg
     9541 acatcagtta aggagttcgc catgaaacat gaccattttg ttgttcaaag cccggataaa
     9601 cctgcgcaac agttgttact gcttttccac ggtgtcgggg ataacccggt ggcaatgggg
     9661 gaaataggca gctggtttgc gccgctattt cctgatgcgc tggtggtgag tgtcggaggc
     9721 gcggagccga gcggtaatcc ggcggggcgt cagtggtttt cggtgcaggg tattacggaa
     9781 gataatcgcc aggcgcgtgt ggatgccatc atgccgacgt ttattgagat ggtgcgctac
     9841 tggcagaaac agagcggagt aggggctaac gccacagcgc taatcggttt ctctcaaggc
     9901 gcgattatgg cgctggagag cattaaagcc gaaccgggcc ttgcttcacg cgtcattgct
     9961 tttaacggac gctatgcgag cctgccggaa actgcgtcta cagccacgac aattcacctg
    10021 attcatggcg gtgaagatcc ggttatcgat ctggcgcatg cggttgcggc gcaggaagcg
    10081 ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg aggatttagg ccatgcgatc
    10141 gacaatcgca gtatgcaatt tgccctggat cacttgcgtt ataccattcc gaaacattat
    10201 ttcgacgaag cgctaagtgg cggtaagcct ggcgatgatg atgtgattga aatgatgtaa
    10261 aagacagatt acctggcctg ataagacgcg tgagcgtcgc atcaggcatc gttcaccatt
    10321 gccggatgcg gcgttaacgc cttatccggc ctacggttga gcactcagct tacttcttcg
    10381 gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg
    10441 ggggaagctc cggcttgttg gcgagaaatt tcttgtggtc gacgcgcttg agatctttaa
    10501 tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa tatttagcca
    10561 gccagtccat gcttatttcc tcttgcagaa ccactcatca ggcgacgagc tgttccatga
    10621 tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat tcattaattt
    10681 tatgaatagt ggcattgacc ggcccgagtt ccaccacctg cgcccccatg cgagcaataa
    10741 agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca ttatagtgct
    10801 caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg
    10861 aaagccacca atccaccgta tagcgcagtt gatgtttttc aagcagggca agcacctgcg
    10921 ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc acaaacagtt
    10981 cacccggaat aacgttgtta ctgcccgttc ccgcctgaat attggcaatc tgcatactgg
    11041 tcgccgggaa gaattcattg ccctgatccc actcaatagc cactaattca ttaaggaaag
    11101 gtgctgcgcg atgtactgga ttgtcagcca gatgtgggta ggccacatgc ccctgcacgc
    11161 catgaatggt aaggttgcag gttaacgatc cgcgacgacc atttttcacc acatcaccaa
    11221 ccacttcgat actcgacggt tcgccgacca gacagtaatc gagacgctca ttacgcgcca
    11281 ttaacgcttc gacgactttt accgtaccgt tgtgggcact ggcttcttca tcagaggtga
    11341 tcagaaatgc cagtcgtccc gtatggttgg gatgttgtgc gacaaaacgt tctgctgcta
    11401 ccaccatcgc cgccagcgag cctttcatat ctgccgcacc gcgcccgaat aacatgccat
    11461 cacgaatggt gggttcaaac ggcggattga tccaacgatc ggcgtcgcca ggcggcacca
    11521 cgtcggtatg cccggcaaag gccaatgttt caccttgccc acgccatgcc caaaaattct
    11581 gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat cgcctgcaaa cgatcaatca
    11641 acaaagcctg gcatcctgcg tcatcaggac tcagggaagg gcggcgaata agctgttgtg
    11701 tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaattgc tgataactgg
    11761 aatcactgaa acccagcagc ataggcttac cgggcgcgca gagcaatgga cgtttgataa
    11821 ttgcaggcat ttcagtcatt aatgccgccg ctgaggccgc atcggtgatt ttattgcggg
    11881 tggtttcgtc cagtttacgc caggttgtac cacgggtgtt gagtaacgcc tcccaaccta
    11941 attcgttgat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg taatcatgaa
    12001 aacgatagtc gatgttattg gcttccagcc aacggcgagc ctttttaatg gtgtcacaat
    12061 ttttgatacc gtaaagtgta accataatga atcctttttc gatttatgag aataaatact
    12121 catttaaggg tgaaaacatt ttgcctgcaa atgattatca ggaaatataa cattatcgaa
    12181 gtattcaaat taacaactat taaataatat taaacatttt taacgtaaac attaagaata
    12241 ttcatcttgt tatctgtctg tattttaacc tgaattaaat cagtattaga tgccgtatga
    12301 atatctcgaa atgttgctgt gtatcacata caaatcatca ccgcaggacc atagtattta
    12361 cataagattt gtccccccgg aggtagtcat cgcagcaatc gagtaaatta gcttcaccac
    12421 ggtatattgt ttccgtagaa tacccataat tataagagag gttgttatga ttgaacgtga
    12481 actggggaac tggaaagact ttatcgaagt tatgcttcgt aagtaattcc tgagacaaag
    12541 tataaacagc aagaacccgc tgagcaggtt cttaatcgga aagttgaaaa aggcgacaca
    12601 ttggcatgtc gcctttttta ttgcttattc cgggcgcggc tttagcggga agcggcggcg
    12661 caccagcaca aagaacagcg gcacgaagta aatagccaga atagtggctg aaatcatccc
    12721 gcccattacg ccagtaccca ccgcatgctg accaccggaa ccggcacccg tgctggttgc
    12781 cattggcaat acgccgaaga taaatgccag cgaggtcatc agaatcgggc gtaaacgctg
    12841 acggcaggcg tggagcgtcg cttcaaacag gtcgtggcct ttttggttca tctcgttagc
    12901 aaactcgacg atcaggatgg cgtttttcgc cgataaacca atgaccgtta acaggcccac
    12961 ctggaagtaa acgtcgtttt ccagcccgcg catccaggtt gccagcagcg cgccgattac
    13021 ccccagcggc acgaccagca ttaccgagaa cggcaccgac cagctttcat acaacgcagc
    13081 cagacacagg aataccacca gcaaggaaat ggcgtacagc gccggagcct gcgcgccgga
    13141 aagtcgctcc tgatacgaca tcgccgtcca ctccagacca aagccgtttg gcagctgctt
    13201 cactaacgat tccataatat ccatcgccgt accggtactg acccccggtg cggcttcccc
    13261 aacaatctca accgcagaat aaccgttata gcgttccaga cgcggcgagc ctgtttccca
    13321 gcgtgaggtc gcgaaagcag agaagggcac catgccgcca tctttatttc ggacatacca
    13381 gagattgatg tcatctggca gcatgcgata cggcgcagct gcctgcacat agactttctt
    13441 cacgcgaccg cgatccataa agtcattcac atagctcgaa ccccaggcgg tttgcagtgt
    13501 gtcgttaata tcgtcgatag caacgcccag cgcctgagct ttacgctggt cgatatcaat
    13561 ctgcaactgc ggactgtcgt cgaggccgtt atggcgcaca cgggttagct ccgggttttc
    13621 cgccgccagc gccagcaact ggttacgtgc tgccatcagc gcatcgtgac ctgctccagc
    13681 gtggtcctgc aactccatat caaaacccgc agaactacca agaccgctaa ttgctggcgg
    13741 gctgctggcg ataacgcgag cttctttaat cttgttaaac gcctttgttg cacgctcgat
    13801 aatggcaaac gaggtgccgg tcttactgtc gcgttcgctc cagtctttca ggcggataaa
    13861 cattcgcgcc acgttttgcc cgttaccccc agggccagaa ccaacggtgg caaacaccga
    13921 catgatgttg tctttttcat gggtgaagta atatttctcg atttgctcaa cgactttcag
    13981 ggtctgttgt tgggttgaac cgctgggcaa ttgtaccgag gtagtaaaca tgccacggtc
    14041 ttccagcggt aagaacgacg tcggcaaacg caggaacagg aacaccatgc cgccaagcag
    14101 caggacataa atcacaatcc agcgcaggct gcggtggaga attttcgcca cccctttttc
    14161 gtagcgttcg gcgttgcggt taaacatctg gttaaaccag gcaaaaaagc ctttttgccc
    14221 gtgatgctca ccttttttca gtggcttaag tagtgtggca cacagagccg gagtgaggat
    14281 catcgctacc agtactgaca gcaccatcgc cgcaacaatg gtaatagaga actggcgata
    14341 gatggcaccg gtggtgccgc cgaagaaggc cattggtaca aataccgccg aaagcaccat
    14401 cgcaatcccg accagtgccc cctggatctg ccccatcgat ttacgtgtgg cttcgcgagg
    14461 agtgagtcct tcctcactca taatacgttc gacgttttcc accaccacga ttgcgtcatc
    14521 caccagcaga ccgatcgcca gtaccatcgc gaacatggtt aaggtgttga cgctgtaacc
    14581 gaaggcgtaa agtacggaga aggttcccat caacaccacc ggcacggcga tagtgggtat
    14641 cagcgtggcg cggaagtttt gcaggaacag atacataacg aggaaaacca gggcgatagc
    14701 ttccagcagc gttttcacca cgtcttcaat ggaggcttta acaaacgagg tggtttcata
    14761 cgccaccttg tattccagtc cgtgcgggaa gtactgcgcc agctcgtcga gacgattgag
    14821 caccagctcc gctgtcgcca tttcgtttgc gccggaggcc agttttaccc ccagcccgga
    14881 ggctggctta ccattgaagc ggctaagata atcgtatttc tccgccccca tttcgacggt
    14941 ggcgacatcg cccagcctta cctctgagcc gtcttgattc acccgcaagg tgatatcgcg
    15001 gaactgttct ggtgtttgca gcagtgactg ggcgttaatg gtggcgttga gcgcctgctt
    15061 atcgacggaa ggtgtaccac caagttgccc aaccgcaatc tgcgcgttct gtgactcaat
    15121 ggcatcagtg acatctttag ccgtcatctg gaaactgttg agtttcgccg ggtccagcca
    15181 gatacgcatg gaatattgcg aaccataggc atcgatatcc ccgacgccat tcacgcggct
    15241 taacgggtcc tgaatattac tggcaacata atcggcaata tcctgtttat ccatcgaacc
    15301 atcggtagag acgaaagcaa tggtcagaat attggtatcg ccggttttac gcaccgtcac
    15361 gccctgattt tgcaccgcct gcggtaactt tcgcatggct gattgcagct ggttttgtac
    15421 ttgctgcacg gcttcgtccg gatcggtgcc tgctttaaaa cttaaagtga cagatgcctg
    15481 accgatgcca ctgctctgag atgacatata catgagatta tcgaggccgg tcatattttg
    15541 ctcgataacc tgggtcacgg tgttttccag cgtctgggcc gatgcgccgg gatagttagc
    15601 ggtcactcgc acattcggtg gcgcgagatc ggggtattgt tcaacgggca atgaaaaaat
    15661 cgccagggtc cctgtcagac acaacaggat tgccagcacc caggcaaaaa tggggcgatc
    15721 aataaagaaa ttcgccatta aaagaggacc tcgtgtttct gcgtatcgtt gtaggcttga
    15781 cttgcttcac tgtagcggca aggtacgagc caatcgtgga gaaaaaaagg agttaatgta
    15841 aattatcatg cccttttata ctgaacctta agtgcaagta ttctttggtc agtaggaggc
    15901 attacatcaa ctgactttcc tcaccctccg cagagcgaaa gctaatactc actaacgtgc
    15961 cgccgccgga aggttgtgaa aaagtcagcg tcccacccag ccgttccgcg cgttcgcgca
    16021 tgatattcag accataatga ccttcgggtt ctttcggttc accgatacca atcccgttat
    16081 cacggatata aaccgtgtga ttgccgtccg gcgcggtgac gcaactgacg gcgatttcgc
    16141 tggcgttggc gtgcttcatc gcattcagca ccgcttcgcg aataatttgc aacaaatgca
    16201 cctgcatttg cgcatccagc gccagggttg gcagacggca gtcgagggtc agtttggcgc
    16261 tggtttgatt ttgtaacgta tccagcattt ccctcaacgc ggaggggaga tccgcctgct
    16321 gcagcgtcag gcggaaagta gtcaacagct cgcgtaactg ccgataagca tcattcaacg
    16381 cctgggaaaa gtcggccatg atactttgtg cggtggcgtt atcttccggt atcgaacgct
    16441 tcagtaacgt caactggata cgtaagtaag aaagtacctg agccagcgag tcgtgcaatt
    16501 cgcgggcgat ggttgcacgt tcttccatca acaataactg ctgaaaatgc ttttgcgcct
    16561 gattaaagta caaaccgcgt cccagcatcg acgaaacgct gttaagcagc ggttctgatg
    16621 atgaaacgtg actattttgc cagtgcagtt cgccgtaaac cgtctcttgc attgtcaccg
    16681 gtaaaatctg catcggcaat tccgggtttg gttgcccttc gctaatccgc cagttttcac
    16741 cgacatttaa ctccagatat tcagccgctt cattgtcgcg aacaatctgc aaaatatggc
    16801 ggaaacaatg cacatcaatc tggcttgtgt tcagcgcctg cgaacattga tacaacacct
    16861 ccagacgacg cttggcctcg tggagatcgc gggtcttttc ttctactgac gcctccagcg
    16921 aaaggtacaa tttatgcagc tcgctcgaca tctggttaaa ggtttttgca agcagaccaa
    16981 gctcattcgg cagactggta tccagcggcg gcgagtcgaa ctgcccgtgt tcaatacgtt
    17041 gactggcggt aaccagctga ttcagcgggg caaccacctg atggcgtata cggcgcaaag
    17101 taaaaaagac cagcgtgaaa ataccgatac cgccagccag ggaaatcgcc accaccagca
    17161 gcattttgcg ttcagcgtag tgctgtaaag ccagcacgaa caggtctatc tgattaacat
    17221 aattattaat attggcctga taccacggca aatcgccctt gctgagccga ttattcatct
    17281 ccagccagtt ggcattcaga tgcgcatagc gagtttttac tgcttctggc acataccaga
    17341 cgttgagatt ggttaatacc gatgaatgca gtgcctgctg aaacaactgg cgatgtgcat
    17401 tgagttgtgg actgccactt tgcaagtcgt agcccaggcg gtaactctgc atacgcagcg
    17461 atccggcaat attgatagcc tcagcgtcgc gcaaactgct cgccagagtt agcagagcga
    17521 tacccgtgga aagaatcgac agcagcacaa tgtaaaaaaa ggcccgggcc agactggccg
    17581 agacgggtcg tttaacaatc acacgcgtct tctccacaaa aaatcattaa tgttccagtt
    17641 cgctaaaacg caatttagtc agtgatggga ttatttgatc tgccacagtt tatagcgaaa
    17701 tagttactta ttctgggcac atgaacattg ccgattcccc tgtaagtcgg gtaataacaa
    17761 cactcatata aagaataagg tttttacaac caaaaaagaa ggtcgttatg aatcgtttta
    17821 ttatggccaa cagtcagcaa tgtctgggtt gtcatgcttg tgaaatcgcc tgtgtcatgg
    17881 ctcacaatga tgagcaacat gtcctgagcc aacaccattt tcatccccga attacggtta
    17941 tcaaacatca gcagcaacgt agtgcagtga cctgtcacca ttgtgaagat gcgccctgcg
    18001 cccgtagctg ccctaatggc gcaatcagcc acgttgatga cagcattcag gtcaatcagc
    18061 aaaagtgtat tggctgtaaa tcctgcgtgg tggcctgtcc gtttggtacg atgcaaatcg
    18121 tcctgacacc cgtcgcggca ggaaaagtaa aagccacggc gcataaatgc gacctttgtg
    18181 cagggcgcga aaacggtcct gcctgtgtcg agaattgccc ggcggacgcg ctgcaactgg
    18241 tcactgacgt cgcactctcc ggcatggcga aatcccgccg cttgcgcacc gcacgtcagg
    18301 agcatcaacc gtggcatgcc agtaccgctg cacaagaaat gccggtaatg agtaaagtcg
    18361 aacaaatgca ggcaacgccc gcgcgtggcg agccggataa actggcgatt gaagcgcgca
    18421 aaaccggttt tgatgaaatt tatctgccat tccgtgccga tcaggcacaa cgggaagcct
    18481 cgcgctgcct taaatgcggc gagcacagcg tttgtgaatg gacctgcccg ctgcataacc
    18541 atattccgca gtggattgaa ctggtgaaag ccggaaacat cgacgccgcc gtcgagcttt
    18601 ctcacctgac caacaccctg ccggaaatta ccggacgcgt ttgtccgcag gaccgtttgt
    18661 gtgaaggtgc ctgtactatt cgcgatgagc acggcgcggt aactatcggc aacattgaac
    18721 gctacatttc agatcaggcg ttggcgaaag gttggcgtcc tgacttaagc catgtcacca
    18781 aagtggacaa gcgggtggcg attatcggtg cgggtccggc agggctggcc tgtgcggatg
    18841 ttctgacccg caatggcgtg gcggtgacgg tgtacgatcg ccatccagaa attggtggct
    18901 tgctcacttt cggcattcct tctttcaaac tggataaatc cctgctggca cgccgtcggg
    18961 aaatcttcag cgcgatgggg attcacttcg aactcaattg tgaagtgggt aaagatgtct
    19021 ctttggattc gcttttggaa caatacgacg cggtcttcgt tggcgtaggc acttaccgtt
    19081 ccatgaaagc gggtttaccc aatgaagatg cgccgggcgt ttatgacgcg ctgccgttcc
    19141 tcatcgccaa cactaaacag gtgatgggac tcgaagagct accggaagag ccgtttatca
    19201 ataccgccgg actcaacgtc gtggtactgg gcggcggcga caccgcgatg gactgtgtgc
    19261 gtaccgcgct gcgccacggc gcgagtaacg tcacctgcgc ttatcgtcgt gatgaagcta
    19321 acatgccagg ctcgaagaaa gaggtgaaga acgcccgcga agagggggcc aacttcgaat
    19381 ttaacgtcca gccggtggcg cttgagctga atgagcaagg tcacgtttgc ggaattcgtt
    19441 tcctgcgcac gcgtcttggc gagccggatg cccaggggcg ccggcgactt gtaccgattg
    19501 agggcagtga atttgtcatg ccagccgatg cggtgattat ggtgtttggc ttcaatccac
    19561 acgggatgcc gtggctggag tcgcacggtg taacggtaga caaatggggc cgcatcatcg
    19621 cggatgtgga aagccagtac cgttaccaga ccaccaatcc gaaaatcttc gccggtggcg
    19681 acgccgtgcg tggtgcggat ctggtggtta ccgcaatggc agaaggacgt catgcggcac
    19741 aggggattat tgactggctg ggggtaaaat cagtcaaatc tcactgatag cctgcgcaga
    19801 caaacccgac ttcacagcgt aatataattg ttcatttcgc gctgtggagt cggtatgacg
    19861 caacaaatca ccctcattaa agacaaaatt ctctccgata actatttcac cctgcacaac
    19921 attacttacg atctcacgcg caaagatggc gaagttatcc gccataaacg tgaagtttac
    19981 gatcgcggta atggtgcgac gatcctcctg tacaacgcga agaaaaagac cgtggttctg
    20041 attcgtcagt tccgtgtcgc tacctgggtt aatggcaatg aaagcgggca gctgattgaa
    20101 acctgcgccg ggctgctgga taacgacgaa ccggaagtgt gcattcgtaa agaagcgatt
    20161 gaagagacgg gctatgaagt gggcgaagtg cgcaaattat ttgaactgta tatgtcgcct
    20221 ggcggtgtga ctgagctaat ccactttttt atcgccgaat acagtgacaa tcagcgcgct
    20281 aacgccggtg gcggtgttga agatgaagat attgaagtgc ttgagctgcc gttcagccag
    20341 gcgctggaga tgatcaaaac cggcgagata cgtgacggta agacggtgtt attgcttaac
    20401 tatttgcaaa cgtcacattt aatggactga aaaataacaa taatatttcg ttgtttatta
    20461 ttggctcaat ccgacaaatc tgattgagcc gcgctacctg cgcaacgaag atacgacttg
    20521 tgctgtttgt ttgaacttct ggggtcgtac cgtccatgcg ctatcgcatt ttccttctct
    20581 tttttttcgc tttgttgccg acgtctttgg tgtgggcggc actagcgcaa cgggcgtttt
    20641 ccgactggca ggtcacctgt aataaccaaa acttctgcgt ggcgcgcaat acgggcgatc
    20701 ataatggact ggtgatgacc ctgagccgca gcgccggggc gcataccgat gccgttttac
    20761 gtattgagcg cggcgggttg aagtcgccgg atgcgtctga aggagagata gcgccacggc
    20821 tgctgttaga tggtgagccg ttggcgttaa gtggtgacaa gtggcggatt tcaccatggt
    20881 tattagtaac cgatgatacg gcaaccatca ccgcgttttt gcagatgatt caggaaggga
    20941 aggcgatcac cttacgcgac ggcaatcaga ccatttctct gagtggctta aaagcggcgt
    21001 tgttgtttat tgatgctcag caaaagcgcg ttggcagtga aaccgcgtgg atcaagaaag
    21061 gggacgaacc gccgctcagc gtaccgcccg cgcctgcgct gaaagaggtc gcggtggtta
    21121 acccaacgcc gacgccactc tcactcgaag aacgcaacga tttgctggat tatggaaact
    21181 ggcggatgaa tggtctgcgc tgctcgcttg atccgttacg tcgtgaggtg aatgtcactg
    21241 cgctgactga tgataaagcg ctgatgatga tcagctgtga ggcaggggcc tataacacca
    21301 ttgatttggc atggattgtg tcgcgtaaaa agccgctagc ctcgcgcccg gttcggttgc
    21361 gtttgccgtt caacaacggg caggagacga atgaactgga actgatgaac gcaacatttg
    21421 atgagaaatc gcgtgaactg gtgaccttag cgaaagggcg cggattaagc gattgtggca
    21481 ttcaggcgcg ctggcgcttt gacggtcagc gttttcgcct ggtgcgttac gccgcagaac
    21541 ccacctgcga taactggcat gggccagacg cctggcccac gttgtggatc acccgttaaa
    21601 caaaatggcg gggacatgta ggccggataa gacgctgtag gccggataag acgcgtcagc
    21661 gtcgcatccg gcaatcagca tccggcgatc actatcaggc acctttcact cccagcacct
    21721 tatgcgcttt tgccacaata ttctcggcgg taaagccaaa gaacgggaac agcttatccg
    21781 ccggagcaga ttccccataa cccgtcatcc cgacaattgc ccctttcaga ccaacatact
    21841 tgtaccagta atcggcaata cctgcttcca ccgccacgcg agccgcaacg ttagaaggca
    21901 acaccgactc ccgatattcc tcatcctggg cgtcgaaaat atcggtcgag ggcagggaaa
    21961 ccacgcgaac attgcgacct tctcctgcta atttctccgc agcttgcagg gtgatttcca
    22021 tctctgaacc ggtggcaatc agaataatat ctggcttacc gccgctgtct ttcagcacat
    22081 aaccgccacg agtaatctct ttaacctgat ccggcgtacg ttccacctgg gccagattct
    22141 gccttgagag gatcagtgcc gtcggtccgt tgtggcgctc aaccgccagc ttccagccca
    22201 ccgccgcttc cacctgatcg cacggtcgcc aggtgctgaa atttggcgtt aagcgcaggc
    22261 tggccagttg ctcaacagcc tggtgcgtcg gaccatcttc gcccaggcca attgagtcgt
    22321 gggtataaac cataatctgc cgcgctttca tcagtgccgc catccgcgcg gcgttacggg
    22381 cgtattcaac aaacatcagg aacgtcgcgg tatacggcac aaagccgccg tggtgcgcga
    22441 tgccgttggc gatagcggtc atgccaaatt cacgcacccc gtagtgaatg tagttgcccg
    22501 ccggatcttc cttcagcgaa acagaacctt tccagatggt caggttgctg ggagccagat
    22561 ccgccgaacc gccgagcagc tccggtagca aggggccata agcgttaagc gtattttgcg
    22621 aagccttacg ggtggcgatt ttcgccggat tcgcctgcaa ctcattgata tacttctgag
    22681 tcgttttctc ccagtccttc ggtaaaccac cgctcatccg acgggtaaac tcttctgcca
    22741 gttgcggatg agccttttta taggcggcaa acttctcatt ccacctctgc tgcgcttttt
    22801 cgcctttttc gcgggcatcc caggcgtgat aaatatcttt agggatctca aatggcggat
    22861 ggtgccagcc cagtttttgc cgtgccagcg ccacttcttc ttcccccagc ggtgcgccgt
    22921 gcgcctcttc cttacctgct ttattcggcg atccaaagcc aatcaccgta cgacagataa
    22981 tcagcgacgg cttatctttc acgctttgcg cttcaaggat cgcttccttc accgcctgcg
    23041 gatcgtgacc gtcgatttca tggatcacat gccagtgata ggcttcaaaa cgttttgccg
    23101 tatcgtcggt aaaccagcct tctgtttcac cgtcgatgga aataccgttg tgatcgtaaa
    23161 aaccaatcag cttgcccagt cccagcgtgc ctgccagcga acagacttcg tgggaaatac
    23221 cttccatcag gcagccgtcg cccataaaca cataggtgaa gtgatcgacg atctcatggt
    23281 ctggctggtt aaactgcgcc gccagtgtac gctctgctat cgccagcccg acggcgttcg
    23341 ccaaaccttg tccaagtgga ccggtggtgg tttcaacgcc tggcgtataa ccaatctccg
    23401 ggtggcctgg ggttttcgaa tgcagttgac gaaagttctt cagctcttcc agcggcaggt
    23461 cgtaaccggt cagatgcagc aaactgtaga gcagcatcga cgcgtgaccg ttggaaagaa
    23521 taaagcggtc gcgatcatac caggttgggt cggtagggtt atgtttaaga aaatcgttcc
    23581 acagcacttc ggcaatatca gccatgccca tcggcgcgcc gggatgacca gaattggctt
    23641 tttgtaccgc atccatactg agtgcgcgaa tcgcattggc aaggtctttt cgggacatat
    23701 aacactccgt ggctggttta tagtttggcg gcaagaagat cttccagttt gcgttgatca
    23761 acggcgaaca gacgaatgcc ttcagacagt ttttctaccg ccatcgcata ctgattgtgc
    23821 tcccaacgga actccgcttc gctcatggga gctgggcgtg ggaacgtctg agaaggtggg
    23881 attaatttac gtaccactgg cgaaactttt tcctgcagct ccttcagcaa attcggtgcg
    23941 atagtcagtc gatcgcagcc ggttaaggcg aggatttgtt cggtgcgacg gaagctcgcg
    24001 cccatcacaa tggtttcata gtggtgttgc ttatagtagt cgtagatatt gcgcaccgat
    24061 ttaacgcccg gatcttcttc caccacatac gggtccatcg gcttgcgtgc ctgataccag
    24121 tcataaatac gcccgacaaa cggcgaaatc agaaaaacgc ctgcttccgc acaggcccgt
    24181 gcctgtgcaa aagaaaacag cagcgtcagg ttgcagttaa taccttcttt ttccagctct
    24241 tctgccgcgc gaattccttc ccaggtcgaa gccagcttga tcagaatgcg tgatttctca
    24301 acgccttgtt gctgatacaa gtccaccaga tggcgcgcct tctcaatact cttttcttta
    24361 tcaaaagaga ggcgtgcatc aacttctgtt gacacgcgac cgggtacgat tttgaggatt
    24421 tcagcaccga aattgaccgc cagtttgtca cacgctgcga ccacctgttg ttcctgggtc
    24481 ttgccatttt ttttacccca ggcgatagca tcgtctatta aatgctcata ttgtgataat
    24541 ccggcagcct tgagtaacag cgaaggattg gtggtggcat cctggggatg ataatggcga
    24601 atggactcaa tatcgccgct gtctgccacg acagtggtga actgtttgat gccgtctaac
    24661 tcgttcatag gaaatactcc ttgaaaagta aagtgttaga tgagtgcgtt aattcacact
    24721 tctgagaaat ttcgctaaac gcatcaaaaa agcatagcag acaggcatgg tattgctgga
    24781 ttaagcaggt aacatcagtg ttataggatt attaccaaaa cattatatta atggtgagag
    24841 tttggacttg ctcaaagtct gtagactccg gcaaggtaat aatgtgcgcc acgttgtggg
    24901 cagggggtgt tgcccacaca ctttatttgt gaacgttacg tgaaaggaac aaccaaatgg
    24961 atgaccagtt aaaacaaagt gcacttgatt tccatgaatt tccagttcca gggaaaatcc
    25021 aggtttctcc aaccaagcct ctggcaacgc agcgcgatct ggcgctggcc tactcacccg
    25081 gcgttgccgc accttgtctt gaaatcgaaa aagacccgtt aaaagcctac aaatataccg
    25141 cccgcggtaa cctggtggcg gtgatctcta acggtacggc ggtgctgggg ttaggcaaca
    25201 ttggcgcgct ggctggcaaa ccggtgatgg aaggcaaggg cgttctgttt aagaaattcg
    25261 ccgggattga tgtatttgac attgaagttg atgaactcga cccggacaaa tttattgaag
    25321 ttgtcgctgc gctcgaaccg accttcggcg gaatcaacct cgaagacatt aaagcgccag
    25381 aatgtttcta tattgaacag aaactgcgcg agcggatgaa tattccggta ttccacgacg
    25441 atcagcacgg cacggcaatc atcagcactg ccgccatcct caacggcttg cgcgtggtgg
    25501 agaaaaacat ctccgacgtg cggatggtgg tttccggcgc gggtgccgca gcaatcgcct
    25561 gtatgaatct gctggtagcg ctgggtctgc aaaaacataa catcgtggtt tgcgattcaa
    25621 aaggcgttat ctatcaaggc cgtgagccaa acatggcgga aaccaaagcc gcatatgcgg
    25681 tggtggatga cggcaaacgt accctcgatg atgtgattga aggtgcggat attttcctgg
    25741 gctgttccgg cccgaaagtg ctgacccagg aaatggtgaa gaaaatggct cgggcaccaa
    25801 tgattctggc gctggcgaat ccggaaccgg aaattctgcc gccgctggcg aaagaagtgc
    25861 gtccggatgc catcatctgc accggtcgtt ctgactatcc aaaccaggtc aacaacgtcc
    25921 tttgcttccc gttcatcttc cgtggcgcgc tggacgttgg cgcaaccgcc atcaacgaag
    25981 agatgaaact ggcggcggta cgcgcgattg cagaactggc ccatgcggaa cagagcgaag
    26041 tggtggcttc agcgtatggc gatcaggatc tgagctttgg tccggaatac atcattccaa
    26101 aaccgtttga tccgcgcttg atcgttaaga tcgctcctgc ggtcgcgaaa gcggcgatgg
    26161 agtcgggcgt ggcgacgcgt ccgattgctg atttcgacgt ctacatcgac aagctgactg
    26221 agttcgttta caaaaccaac ctgtttatga agccgatttt ctcccaggct cgcaaagcgc
    26281 cgaagcgcgt agttctgccg gaaggggaag aggcgcgcgt tctgcatgcc actcaggaac
    26341 tggtaacgct gggactggcg aaaccgatcc ttatcggtcg tccgaacgtg atcgaaatgc
    26401 gcattcagaa actgggcttg cagatcaaag cgggcgttga ttttgagatc gtcaataacg
    26461 aatccgatcc gcgctttaaa gagtactgga ccgaatactt ccagatcatg aagcgtcgcg
    26521 gcgtcactca ggaacaggcg cagcgggcgc tgatcagtaa cccgacagtg atcggcgcga
    26581 tcatggttca gcgtggcgaa gccgatgcaa tgatttgcgg tacggtgggt gattatcatg
    26641 aacattttag cgtggtgaaa aatgtctttg gttatcgcga tggcgttcac accgcaggtg
    26701 caatgaacgc gctgctgctg ccgagtggta acacctttat tgccgatacc tacgtcaatg
    26761 acgaaccaga cgcagaagag ctggcggaga tcaccttgat ggcggcagaa actgtccgtc
    26821 gtttcggtat tgaaccgcgc gttgctctgt tgtcgcactc caactttggt tcttctgact
    26881 gcccgtcgtc gagcaaaatg cgtcaggcgc tggaactggt cagggaacgt gcgccagaac
    26941 tgatgattga tggtgaaatg cacggcgatg cggcgctggt ggaagcgatt cgcaacgacc
    27001 gtatgccgga cagccctttg aaaggttctg ccaatattct ggtgatgccg aacatggaag
    27061 ctgcccgcat tagttacaac ttactgcgcg tttccagctc ggaaggtgtg actgtcggtc
    27121 cggtgctgat gggcgtggcg aaaccggttc acgtgttaac gccgatcgca tcggtgcgtc
    27181 gtatcgtcaa catggtggcg ctggccgtgg ttgaagcgca aacccaaccg ctgtaattct
    27241 tttttaactc tcacgcttac cctgaatatt cagggtaagc ggtttggctg caatatatta
    27301 gtaaagctta ttactgagtt tgcgaataat aaaaaaagca gtctatataa tatctcgata
    27361 ttattttatt tatattcatg cgttgcatat gaaagtttat gcaccacagc gaatatctct
    27421 ccattcttag tgatctacct caccttttaa acgcgcttgc cgaattttgt tatttactct
    27481 gacgaaaaat tgtcacgata cacgaaagtt tttcacaggc ggcgactcat ggataaagaa
    27541 cgcatcattc aggaatttgt gcccggcaaa caggtcacac tggcgcatct catcgcgcat
    27601 cccggtgagg aactggcgaa gaaaatcggc gttccggatg cgggcgtaat tggcattatg
    27661 acgctaactc ccggcgaaac ggcgatgatc gtcggtgatt tagcgctgaa agccgctgat
    27721 gtgcatatcg gttttctcga ccgcttcagc ggtgcattgg tgatttatgg ctcggtgggc
    27781 gcagtagaag aagcgttatc acaaaccgtc agcggtttag gtcgcttatt aaattatacg
    27841 ctttgtgaaa tgaccaaaag ttaatacgag gcattcatga aacgaattgc gtttgtgggt
    27901 agcgtcgggg caggaaaaac aacgttattt aatgcgttgc aggggaatta taccctcgcc
    27961 agaaaaacac aggccgtgga atttaatgat aagggcgata ttgatacgcc gggtgaatat
    28021 tttagccatc cccgctggta tcacgcctta attaccacgc tacaggatgt agacatgctg
    28081 atctatgtcc atggcgcaaa tgacccggaa agtcggttgc ctgccgggtt gctggatatt
    28141 ggcgtcagca aacggcaaat cgccgtcatc agtaaaacgg acatgccaga tgctgacgtc
    28201 gccgcaacgc gaaagttatt gcttgagacg ggtttcgaag agccgatttt cgagctaaac
    28261 agccacgacc cgcaaagcgt gcagcaactg gtggattatc tggcatctct caccaaacag
    28321 gaggaagcag gtgaaaaaac tcatcacagc gaatgatatt cgtgaagcac acgcgcgcgg
    28381 cgaacaggca atgtccgtgg ttctgcgcgc cagcattatt accccggaag cccgtaaagt
    28441 ggcggatcta ctgggtttta ccatcaccga atgtgatgaa tcgatcccgg taacggcgtc
    28501 cgtgcccgcc agcgtgccag ccgacaaaac cgaaagccag cgcatccgcg aaaccatcat
    28561 tgcccagctg ccggaaggcc agtttaccga aagcctggtc gcacagctga tggaaaaagt
    28621 gatgaaggaa aaacagtcgc tggagcaggg cgcaatgcag ccgagcttca aatcggtgac
    28681 cggcaaaggc ggcatcaaag tgattgatgg cagcagcgtc aagtttggtc gttttgatgg
    28741 cgcacaaccg cattgcgtgg gcttgactga tcttgtgacc ggagacgacg gcagcagcat
    28801 ggccgccggt tttatgcagt gggagaacgc attcttcccg tggaccctga actacgacga
    28861 aatcgacatg gtgctggaag gggagctcca tgttcgccac gaaggcgaaa ccatgatcgc
    28921 caaagcaggc gacgtgatgt ttatcccgaa aggctccagc atcgaatttg gtacgacatc
    28981 cagcgtgaaa ttcctgtatg tcgcctggcc ggctaactgg caatccctat gaaagatttc
    29041 atcaccgaag catggctaag agcgaaccat acgctcagcg aaggcgcaga gatccatctc
    29101 cctgcggaca gtcgcctgac gccctctgcc cgggagttgc tggaaagccg ccatctgcgc
    29161 atcaagttta ttgacgagca gggccgcctg tttgttgacg atgaacagca gcagccgcag
    29221 cccgttcatg ggctgaccag tagcgatgaa catccgcagg cgtgctgcga actgtgccgc
    29281 cagccggtgg caaaaaagcc ggatacgctg actcacctgt cggcggaaaa aatggtcgcc
    29341 aaaagcgatc cgcgtctggg ttttcgcgcg gttctcgaca gcaccattgc gctggcagtg
    29401 tggctgcaaa ttgaactggc ggaaccgtgg cagccgtggc tggcggatat ccgttcgcgt
    29461 cttggcaaca ttatgcgcgc cgatgcgctg ggggaaccgc tgggctgcca ggcgattgtc
    29521 gggctttctg acgaagatct gcatcgactt tctcaccagc cgctgcgcta tctcgaccac
    29581 gatcatctgg tgccggaagc cagccacggg cgcgatgcgg cgttgctcaa tctgctacgt
    29641 accaaagtgc gggaaaccga aaccgtagcg gcgcaggtgt ttatcacccg cagctttgaa
    29701 gtattacgcc cggacatctt gcaggcgctg aaccgccttt cgagcacggt ctacgtaatg
    29761 atgatcctga gcgtaacgaa gccgccgcta acggtgaagc agatccaaca acgactgggg
    29821 gaaacgcaat gattattgaa cgttgtcgtg aactggcgtt gcgagcgccc gccagagtgg
    29881 tttttccgga tgcgttagat caacgtgtgc tgaaagctgc gcaatattta catcaacaag
    29941 gtctggcaac gcccattctg gtcgctaatc cgtttgaact tcgtcagttt gcgctcagtc
    30001 acggcgtggc gatggacggg ctacaggtga ttgatccgca tggcaacctc gcaatgcggg
    30061 aagaatttgc tcatcgctgg ctggcccgcg cgggcgaaaa aacgccgccg gatgcgctgg
    30121 aaaaacttac cgatccgctg atgttcgccg cagcaatggt cagcgccggt aaagcggatg
    30181 tctgtatcgc gggcaacctc tcttccacgg cgaatgtgct gcgtgccgga ttacgtatta
    30241 tcggcttgca gccaggctgt aaaacgctct catccatttt cctgatgctg ccacagtaca
    30301 gcggtccggc gttgggcttt gccgattgca gcgtggtgcc acagccgacg gcggcgcaac
    30361 tggcggatat cgcgcttgcc agtgccgaaa cctggcgcgc cctcaccgga gaagagccgc
    30421 gcgtggcgat gctgtcgttt tccagtaacg gtagcgcccg tcacccctgc gttgccaacg
    30481 tccagcaggc gacagaaatc gtccgtgagc gcgcaccaaa gctggtggtt gatggcgagt
    30541 tgcagtttga cgccgccttc gtgccggaag tggcggcgca aaaagcgcct gccagcccgc
    30601 tacagggcaa agccaatgtg atggtttttc cgtcgctgga agccggaaat attggctaca
    30661 aaatcgcaca acgactcggc ggatatcgtg ccgtcgggcc attgatacaa ggacttgccg
    30721 cgccgatgca cgatctctct cgtggttgta gcgtgcagga aattatcgag ctggcgctgg
    30781 tggcagctgt gccgcgtcag acagaagtga accgcgaaag cagtttacaa acactggttg
    30841 aatgaacggt cccgttctgg accctgataa agaggaaagc acaatggaag cattaggaat
    30901 gatcgaaacc cggggcctgg ttgcactgat tgaggcttct gacgcgatgg ttaaagctgc
    30961 gcgcgtcaag ctggttggtg ttaagcagat tggcggcggc ctgtgtactg ccatggttcg
    31021 tggtgatgtt gcagcctgta aagccgcgac cgatgcaggt gccgcagcgg cacagcgtat
    31081 cggcgagctg gtatctgtac acgttatccc gcgtccgcac ggcgacctgg aagaagtctt
    31141 cccgatcggc ctgaaaggcg atagcagcaa cctgtaatca ccctgtgtga cgtcgattcg
    31201 ggcgtcacgc tctctccttt ttcattttac cttctgcgga gggtaggggg agcttttacc
    31261 tgaaatacgc cacggaggcg ggtatgaaac tggcagtcgt cactggacaa attgtttgta
    31321 ccgtacgcca tcacggactg gcgcatgaca aattgctgat ggtggaaatg attgatccac
    31381 aaggtaatcc tgacgggcaa tgcgccgtcg ccatcgacaa tattggcgcg ggaaccgggg
    31441 agtgggtgtt actggtgagt ggcagttccg cccgccaggc gcataaaagc gaaacgtcac
    31501 cggtcgatct gtgcgtgatt ggcattgtcg atgaggtggt gtctggcggt caggtaattt
    31561 tccacaaata aggcagaaca tcatgaatca acaggatatt gaacaggtgg tgaaagcggt
    31621 actgctgaaa atgcaaagca gtgacacgcc gcccgccgcc gttcatgaga tgggcgtttt
    31681 cgcgtccctg gatgacgccg ttgcggcagc caaagtcgcc cagcaagggt taaaaagcgt
    31741 ggcaatgcgc cagttagcca ttgctgccat tcgtgaggca ggcgaaaaac acgccagaga
    31801 tttagcggaa cttgccgtca gtgaaaccgg catgggccgc gttgaagata aatttgccaa
    31861 aaacgtcgct caggcgcgcg gcacgccagg cgttgagtgt ctctctccgc aggtgctgac
    31921 cggcgacaac ggcctgactt taattgaaaa cgcgccctgg ggcgtggtgg cttcggtaac
    31981 tccgtccact aacccggcgg caaccgtaat taataacgcc atcagcctga ttgccgcggg
    32041 caacagcgtc atttttgccc cgcatccggc ggcgaaaaaa gtctcccagc gggcgatcac
    32101 gctgctcaac caggcgattg ttgccgcagg cgggccggaa aacttactgg ttactgtggc
    32161 aaatccggat atcgaaaccg cgcagcgttt gttcaagttt ccgggcatcg gccttctggt
    32221 ggtgaccggc ggcgaagcgg tagtagaagc ggcgcgtaaa cacaccaata aacgtctgat
    32281 tgccgcaggc gctggcaacc cgccggtagt ggtggatgaa accgccgacc tcgcccgtgc
    32341 cgctcagtcc atcgtcaaag gcgcttcttt cgataacaac atcatttgtg ccgacgaaaa
    32401 ggtactgatt gttgttgata gcgtagccga tgaactgatg cgtctgatgg aaggccagca
    32461 cgcggtgaaa ctgaccgcag aacaggcgca gcagctgcaa ccggtgttgc tgaaaaatat
    32521 cgacgagcgc ggaaaaggca ccgtcagccg tgactgggtt ggtcgcgatg cggcgaaaat
    32581 cgcggcggca atcggcctga acgtgccgca agaaacgcgt ttgctgtttg tggaaaccac
    32641 tgcagaacat ccgtttgccg tgaccgaact gatgatgccg gtgctgcccg tcgtgcgcgt
    32701 cgccaacgtg gcggatgcca ttgcgctggc ggtgaaactg gaaggcggtt gccaccacac
    32761 ggcggcaatg cactcgcgca acatcgaaaa catgaaccag atggcgaatg ctattgatac
    32821 cagcattttc gttaagaacg gaccgcgcat tgccgggctg gggctgggcg gggaaggctg
    32881 gaccaccatg accatcacca cgccaaccgg tgaaggggta actagcgtgc gtacgtttgt
    32941 ccgtctgcgt cgctgtgtat tagtcgatgc gtttcgcatt gtttaaggag taacagatgg
    33001 cgcacgacga acaatggctc accccacgtc tgcaaacggc ggcaacgctg tgtaaccaga
    33061 cgcccgccgc gacggaatct ccgctgtggc tgggggtgga tctgggcacc tgcgatgtgg
    33121 tgtcgatggt tgtcgaccgc gacggtcagt cggtggcggt atgcctcgac tgggccgatg
    33181 tggtccgcga cggcatcgtc tgggatttct tcggcgctgt caccattgtt cgtcgccatc
    33241 tcgacacgct cgagcagcaa ttcggtcgcc gttttagcca tgcggcgact tcatttcctc
    33301 caggtacgga cccgcgtatt tccatcaacg tgctggagtc tgctggactg gaagtgagcc
    33361 atgtgctgga tgagccaacc gcagttgcgg atctgctgca actggacaac gccggtgtag
    33421 tggatatcgg cggcggcact accggcattg ccatcgtgaa aaagggcaag gtgacgtact
    33481 cggcggatga agcaaccggc ggacatcaca tctctctgac tctcgccgga aatcgccgta
    33541 tttcgctgga agaggcggag cagtacaagc gcggtcacgg tgaagagatt tggcctacgg
    33601 tgaaaccggt gtacgaaaaa atggtggaca ttgtcgcccg gcatattgaa gggcagggca
    33661 ttactgattt atggctggcg ggcggctctt gtatgcaacc gggcgtggcg gaactgtttc
    33721 gcaaacaatt cccggcgtta caggtgcatt taccgcagca cagtttgttt atgaccccgc
    33781 tggcgatcgc cagtagtggg cgagagaaag cggaggggct atatgcaaaa tgaattgcag
    33841 accgcgctct ttcaggcgtt cgataccctg aatctgcaac gggtaaaaac atttagcgtt
    33901 ccaccggtga cgctttgcgg tccgggctcg gtgagcagtt gcggacagca agcgcaaacg
    33961 cgtgggctga aacatctgtt cgtgatggca gacagctttt tgcatcaggc agggatgacc
    34021 gccgggctga cgcgcagcct ggctgttaaa ggcatcgcca tgacgctctg gccatgtccg
    34081 gtgggcgaac cgtgcatcac cgacgtgtgt gcagccgtgg cgcagttgcg tgagtcaggc
    34141 tgtgatgggg tgatcgcatt tggcggcggc tcggtgctgg atgcggcgaa agccgtggcg
    34201 ttgctggtga cgaacccgga tagcacgctg gcagagatgt cagaaaccag cgttctgcaa
    34261 ccgcgcttgc cgctgattgc cattccaacg accgccggaa ccggctctga aaccaccaat
    34321 gtaacggtga ttatcgacgc ggtgagcggg cgcaagcagg tgttagccca tgcctcgctg
    34381 atgccggatg tggcgattct cgacgccgcg ttgaccgaag gtgtgccgtc gcatgtcacg
    34441 gcgatgaccg gcattgatgc gttaacccat gccattgaag catacagcgc cctgaacgct
    34501 acaccgttta ccgacagtct ggcgattggt gccattgcga tgattggcaa atcgctgccg
    34561 aaagcggtgg gctacggtca cgaccttgcc gcgcgcgaga gcatgttgct ggcttcatgt
    34621 atggcgggaa tggcgttttc cagtgcgggt cttgggttgt gccacgcgat ggcgcatcag
    34681 ccgggcgcgg cgctgcatat tccacacggt ctggcgaacg ccatgttgct gccaacggtg
    34741 atggaattta accggatggt ttgtcgtgaa cgctttagtc agattggtcg ggcactgcga
    34801 actaaaaaat ccgacgatcg tgacgctatt aacgcggtaa gtgagctgat tgcggaagtt
    34861 gggattggta aacgactggg cgatgttggt gcgacatctg cgcattacgg cgcatgggcg
    34921 caggccgcgc tggaagatat ttgtctgcgc agtaacccgc gtaccgccag cctggaacag
    34981 attgtcggac tgtacgcggc ggcgcaataa atgccggatg cgacgcttgc cgcgtcttat
    35041 ccagcctacg ggattgcaca tgtagggcgg ataaggcgtt cacgccgcat ccgccagtgg
    35101 cgcggtgcaa ttgccggatg cgacgcttgc cgcgtctgat ccggccttcg ggattgcaca
    35161 tgtagggcgg ataaggcgtt tacgccgcat ccgccaataa ataacaacta acagggagta
    35221 aaggcgatgg gaattaacga aatcatcatg tacatcatga tgttctttat gctgatagct
    35281 gccgtagaca ggatcctgtc gcagttcggc ggttctgctc gtttcctcgg taagttcggt
    35341 aaaagtatcg aaggatcagg cggtcagttc gaagaaggct ttatggcaat gggcgctctg
    35401 ggtctggcga tggtcggtat gaccgcgctg gcaccggtac tggctcacgt actcgggccg
    35461 gtaattatcc cggtttacga aatgctcggc gcaaacccgt caatgttcgc cggaacgctg
    35521 ctggcgtgcg atatgggcgg cttcttcctc gccaaagagc tggcgggcgg cgacgtagca
    35581 gcgtggctat actctgggtt aattcttggg tcgatgatgg ggccaacgat tgtgttttcc
    35641 attccggtgg cgctcggcat tatcgaacct tctgaccgtc gttatctggc gctcggcgtg
    35701 ctggcgggca ttgtgaccat tccgattggc tgtattgctg gtggtctggt tgctatgtac
    35761 tccggtgtgc agatcaacgg tcagccggtg gaattcactt tcgccctgat cctgatgaac
    35821 atgatcccgg tgatcattgt tgcgattctg gtggcgctgg ggctgaaatt catcccggaa
    35881 aaaatgatca acggcttcca gatcttcgcc aaattcctcg ttgcattgat caccctcggt
    35941 cttgctgccg cggtagttaa gtttctgctt ggctgggaac tgatccccgg tctggaccct
    36001 atctttatgg cccctggcga taaacccggt gaagtgatgc gcgccattga agttatcggt
    36061 tctatctcct gcgttctgtt aggggcgtat ccaatggtgc tgctgctgac ccgctggttt
    36121 gaaaaaccgc tgatgagcgt cggtaaagtg ctgaatatga acaacatcgc ggcagccggc
    36181 atggtggcaa cgcttgccaa caacatcccg atgttcggca tgatgaagca gatggatacc
    36241 cgcggcaaag tcatcaactg cgccttcgcc gtttccgctg ctttcgccct gggcgaccac
    36301 ttaggcttcg ccgctgccaa catgaacgcc atgatcttcc cgatgattgt cggcaagttg
    36361 atcggcggcg taacggcgat tggcgtggcg atgatgctgg tgccaaaaga agacgcgacc
    36421 acggctaaaa ccgaagcgga ggcacaatcg tgaacactcg ccagctattg agcgtcggta
    36481 tcgatatcgg caccaccacc acccaggtga ttttctccca cctggagctg gttaaccgtg
    36541 cggcggtgtc gcaggtgccg cgctacgaat tcattaaacg cgaaattagc tggcaaagcc
    36601 cggtgttctt tacccctgtc gataaacagg gtggtttaaa agaagcggaa ctgaaaacct
    36661 taatcctcga gcaatatcag gctgcgggca ttgcgccgga aagcgttgat tctggtgcca
    36721 tcatcatcac cggtgaaagc gcgaaaaccc gcaatgctcg tccggcggtg atggtgctct
    36781 cccagtcgct gggcgatttt gtcgttgcca gcgccgggcc gcacctcgaa tccgtcattg
    36841 ccggtcacgg tgccggggca caaacccttt ctgaacaacg gctgtgtcgg gtactgaata
    36901 ttgacatcgg cggtggcact gcgaactacg ccctgttcga tgccggaaaa atcagcggca
    36961 ctgcctgcct caacgtcggt ggtcgcctgc tggaaaccga cagccagggg cgcgtggttt
    37021 acgctcataa accggggcag atgattgtgg atgagtgctt cggcgcaggc accgacgccc
    37081 gttcgctgac cggcgcgcag ctggtgcagg ttacccggcg gatggcagaa ctgattgtcg
    37141 aagtgattga cggaacgctc tcgccgctcg cgcaggcatt gatgcaaacc ggtttgctgc
    37201 ccgcaggtgt tacgcccgaa atcattacgc tttctggcgg cgtgggcgaa tgttatcgcc
    37261 accagcccgc cgacccgttc tgttttgccg atattggccc gctgctggca acggcgctgc
    37321 atgaccatcc gcgcctgcgt gagatgaacg tacagtttcc ggcgcaaacc gtgcgcgcca
    37381 cggtgattgg cgcgggcgcg catacccttt cgctctctgg cagcacaatc tggcttgagg
    37441 gcgtacaact gccgctgcgc aatttgccgg tggcgatccc gattgatgaa acggatctgg
    37501 tgagtgcctg gcaacaggcg ctgcttcagc tggatcttga tcccaaaact gacgcgtacg
    37561 tgctggcgct tcccgcctcg ctgcctgtgc gttacgccgc ggtactgacg gtcatcaacg
    37621 cgctggtcga tttcgtcgcg cgttttccaa atccgcatcc cctgctggtg gtggccgggc
    37681 aggactttgg taaagctctg ggcatgttgt tgcgcccaca gctacaacaa ctcccgttgg
    37741 cagtcattga cgaagtgatt gtccgcgcgg gggactatat cgacattggt acgcctcttt
    37801 ttggcggatc ggttgtgccg gtgacggtga aatcactcgc atttccttcc tgagggaacg
    37861 acttatgaaa ctaaagacca cattgttcgg caatgtatat cagtttaagg atgtaaaaga
    37921 ggtgctggct aaagccaacg aactgcgttc gggggatgtg ctggcgggcg ttgcagcggc
    37981 aagctcacag gagcgcgtgg cggcaaagca ggtgttgtcg gaaatgaccg tagcggacat
    38041 ccgcaataat ccggtgattg cctatgaaga tgactgcgtg acgcggctga ttcaggacga
    38101 tgttaacgaa acggcctaca accagattaa aaacaggagc atcagcgaac tgcgtgagta
    38161 tgtgctgagc gatgaaacca gcgtggacga cattgccttt acccgcaaag ggctgacctc
    38221 ggaagtggtc gcggcggtag cgaagatttg ctccaacgcg gacctgatct acggcgcgaa
    38281 gaaaatgccg gtaatcaaaa aggccaatac caccatcggt attccgggca ccttcagcgc
    38341 ccgtttgcag ccaaatgaca cccgtgacga cgtgcaaagt atcgctgcac aaatctacga
    38401 agggctttcc ttcggggtgg gcgatgcggt gatcggcgtt aacccggtaa ctgacgacgt
    38461 ggaaaactta agccgcgtgt tggacaccat ttacggcgtg atcgacaaat tcaacatccc
    38521 aactcagggc tgcgtactgg cgcacgtcac cacccagatc gaagcgattc gtcgcggcgc
    38581 gcctggcgga ctgattttcc agagtatctg tggcagcgaa aaagggctga aagagtttgg
    38641 cgtggaactg gcgatgctcg acgaagcgcg cgcagtgggt gctgagttca accgtatcgc
    38701 cggggaaaac tgcctctact tcgaaaccgg gcaaggttcg gcgctgtctg ctggcgctaa
    38761 cttcggcgca gactaggtga cgatggaagc acgtaactat gggctggcgc gtcattacga
    38821 tccgtttatc gtcaacaccg tggtcggctt tattgggccg gagtatctct acaacgaccg
    38881 ccagattatc cgtgctggct tagaagatca ctttatgggc aagctgagcg gcatctctat
    38941 gggctgtgac tgctgttaca ccaaccacgc tgatgctgac cagaacctca acgaaaacct
    39001 gatgatcctg ctcgccaccg caggctgcaa ctacatcatg gggatgccgc tgggcgatga
    39061 catcatgctc aactatcaga ccaccgcatt ccacgacact gccactgtgc gtcagttact
    39121 caacctgcgc ccgtcaccgg agtttgaacg ctggctggaa agcatgggca ttatggcaaa
    39181 cggtcgcctg accaaacggg cgggcgatcc gtcactgttc ttctgatgac gcggagataa
    39241 cacatcatgg atcaaaaaca gattgaagaa attgtacgca gcgtgatggc gtcaatggga
    39301 caagcggccc ccgcgccgtc agaagcaaag tgcgccacca ctaactgtgc ggcaccggtg
    39361 acctcggaaa gctgcgcgct ggatttaggt tctgcggaag caaaagcgtg gattggtgtt
    39421 gaaaacccgc atcgcgcaga cgtattaaca gaactgcgcc gcagcaccgt ggcccgcgtt
    39481 tgtaccggtc gtaccggtcc gcgtccacgt acgcaggcgc tgctgcgttt cctggccgat
    39541 cactctcgtt cgaaagatac cgtactgaaa gaagtgccgg aagagtgggt gaaagcgcag
    39601 ggcttgctgg aagtacgctc cgaaattagc gacaaaaatc tctacctgac tcgcccggat
    39661 atgggccgcc gcctgtgtgc agaagctgtt gaagcgctga aagcacagtg tgttgctaat
    39721 ccggacgtac aggtcgttat ttctgatggc ctgtcaactg atgccatcac cgtgaactac
    39781 gaagagatcc tgccgccgct gatggcgggc ctgaaacagg ccgggctgaa agtcggcacg
    39841 ccattctttg ttcgttatgg tcgcgtgaag attgaagatc agatcggtga gatcctcggc
    39901 gcgaaagtgg tgatcctgct ggtgggggaa cgtccggggt tgggtcagtc agaaagcctc
    39961 tcctgttacg ccgtttactc gccgcgtatg gcgaccaccg ttgaggccga tcgcacctgc
    40021 atctctaaca ttcaccaggg cggcacgccg ccggttgaag ccgcagccgt cattgtggat
    40081 ttggcgaaac gtatgctgga gcagaaagca tccggcatca acatgacccg ataaggaggc
    40141 atcatgccag ctttagattt gattcgaccg tcggtaaccg ctatgcgggt gattgcctcc
    40201 gttaacgctg aatttgcgcg tgaactaaaa ttgccgccgc atattcgtag cctcgggctg
    40261 atttctgctg attccgatga cgtcacctac attgcggctg acgaagcgac caagcaggcg
    40321 atggtcgaag tggtatatgg ccgctcgctg tatgcgggag cagcacacgg accgtcaccg
    40381 actgccggtg aagtgctgat tatgcttggt gggccaaacc cggcggaagt gcgtgctggt
    40441 ctggatgcga tggttgcgca tattgaaaat ggcgctgctt tccagtgggc taatgacgca
    40501 gaaaacaccg cattcctggc acatgtagtt tcgcgtaccg gttcttatct ttcatcaacc
    40561 gccgggatca cgcttggcga tccgatggcg tatctggtgg caccgccgct ggaagcgacc
    40621 tacggcattg atgcagcgtt gaaatctgcc gacgtacagc tggtgaccta tgtcccgcca
    40681 ccgtctgaaa ccaactactc ggcagcattt ttaaccggta gccaggccgc gtgtaaagca
    40741 gcctgtaacg cctttaccga tgcagtgctg gaaatcgcgc gtaatccaat ccagcgtgcg
    40801 taacggaggt tgccgatgat caatgcactg ggattgctgg aagtggacgg catggtcgcc
    40861 gcgatagatg cagcggatgc catgctcaaa gcagctaacg ttcgtctgct cagtcacgaa
    40921 gtgcttgacc ctggtcgctt aacgctggtg gtggaaggcg atctggcggc gtgtcgtgcg
    40981 gcgctggacg caggttgtgc tgccgcgatg cgtaccgggc gtgtcatcag ccgcaaggag
    41041 atcggtcggc cagacgatga cacccagtgg ctggttactg gctttaaccg ccagccgaag
    41101 caacccgtaa aggaacccga cgcgccagtt atcgtcgcgg aatctgctga cgagttgttg
    41161 gcgctgttaa catcagtacg tcagggaatg acggcaggag aagtggctgc ccactttggc
    41221 tggccgctgg aaaaagccag aaatgcgctc gaacagctct tttctgccgg gacgttacgt
    41281 aaacgcagta gtcgttatcg tctcaagccc cattaacctg tcggaggtgc cgggtgtcct
    41341 acaacacccg gcattaacat catgaaaaag acccgtacag ccaatttgca ccatctttat
    41401 catgaaccct tacccgaaaa cctgaagctc acgccgaagg tcgaagtgga taatgttcat
    41461 caacgacaga caacggatgt ctatgaacat gctttgacga ttaccgcctg gcagcagatt
    41521 tacgatcagc tgcatccggg caagtttcat ggtgaattta cggaaattct gctcgatgat
    41581 attcaggttt ttcgtgaata caccggtctg gcgctgcgtc agtcgtgcct ggtctggccg
    41641 aactcgttct ggtttggcat tccggcgacg cgcggtgagc agggatttat cggttcgcaa
    41701 tgtctgggaa gcgcagaaat agcgacgcgc cccggtggaa ccgagtttga attaagtacg
    41761 ccggacgatt atacgatcct gggcgtagtg ctgtctgaag atgtcatcac ccgtcaggct
    41821 aactttttgc acaatccgga tcgggtatta catatgctgc gtagccagtc ggcgctggaa
    41881 gtgaaagagc agcataaagc cgcgctgtgg ggctttgtcc aacaggcgct ggcgacgttt
    41941 tgcgagaacc cggaaaatct ccatcagcct gcagtgcgaa aagtgctggg ggataatttg
    42001 ctaatggcga tgggggcgat gctggaagac gcgcaaccaa tggtgacggc ggaaagcatc
    42061 agtcatcaga gttaccgtag attactttcc cgcgcccgtg aatatgtgct ggaaaatatg
    42121 tctgagctgg tgacggtgct ggacttgtgt aatcaactgc atgtcagtcg ccgcacgcta
    42181 caaaacgcgt ttcacgctat tttaggcatt ggcccaaacg cgtggctgaa acgcattcgc
    42241 ctgaacgccg tacgccgcga actgataagc ccgtggtcgc aaagcacaac ggtaaaagac
    42301 gccgccatgc agtggggatt ctggcatctg gggcaatttg ccacggatta ccagcagctg
    42361 tttgccgaga agccgtcgtt gacgttgcat cagcggatgc gggaatgggg gtgagggagt
    42421 tacacccaat ccctgacctt aataaactca cttaacgccg cttctgggct gccatctttt
    42481 ggctgataat catactccca gcgtaccagt ggcggcattg acatcaggat cgactcggtg
    42541 cgcccgccag tttgcaggcc aaacagagtg ccgcgatccc agaccagatt gaactcgaca
    42601 taacgaccgc gacggtagag ctgaaaattg cgctcgcgct cgccgtaggc catcgcttta
    42661 cgtcgctcta caattggtaa ataagcgtcg gtgtagcctt tgcctaccgc ctgcataaag
    42721 gcaaaacagt ggtcgaaatc tggcgtgttc agatcatcaa agaacagccc gccaataccg
    42781 cgctgttcgt tgcgatgttt gaggtagaag taatcgtcgc accacttttt gtaacgggga
    42841 taaacgtctt caccaaatgg caggcacagg tcacgggcgg tgcggtgcca gtgaatggcg
    42901 tcttcttcaa aaccatagaa aggggttaaa tcgaagccgc cgccaaacca ccagacgggc
    42961 tctgcacccg gtttttcggc aataaaaaaa cgcacattcg cgtggctggt gggaacatac
    43021 gggttatgcg gatgcactac cagtgaaacg cccatcgcct cgaaactgcg cccggcaagt
    43081 tccgggcgat gagcggtggc ggaagcaggc atcgcctcgc cgtggacgtg agaaaagttg
    43141 acgcctgcct gttcgaaaac accaccatta cgcaacaccc gactacgccc gccgccgcca
    43201 gcttcgcgct gccaactatc ttcgacaaat tctgcgccat cgacggcggt cagctgctga
    43261 caaatcgtat cctgaaggtt gagcagaaac tgtttaacct ggtgtgcgtc gggtttcata
    43321 acttatcgct ttttcgaatg tgctttctgg ttgtcgaacc agtggaaata actgatcacg
    43381 ccttcagcaa tcgctgtggc gattttctga cgaaacgccg ccgtgcctaa cagccgctct
    43441 tcttccgggt tggtgataaa cgaggtttcc accagcaccg aaggaaccga cggcgatttc
    43501 aacaccacaa acgcggcttg ttcggtgttg cggctgtgca gtttatgcac cggcttaatc
    43561 tttttcagaa tatgcgagcc aagcgtcagg ctgtttttaa tagtatcggt ttgcaccaga
    43621 tcaaacagca cttgctgcaa taggtgatcc ttgtcagtcg cctttttacc ggcaacttca
    43681 tcggcgcggt tttcgcgttc agacaggtat ttcgccattg cgctacttgc cccacggtta
    43741 gagagggcaa ataccgaagc accggcagct ttcgggttgg taaagccatc ggcatgaatt
    43801 gacataaaca gatctgcgcc atgtttatgg gcgatttcaa cgcgatcgta aagtgggata
    43861 aacgtatcgc cagaacgcgt taaacgcgca tcaatcccat gattacgcaa aatggaacgg
    43921 acgtttttag caatcgccag caccacatgt ttttctttcg aaccgttgcg tccgatcgct
    43981 ccggtatcaa ttccgccgtg gcctggatcg agaacaacga cacgtttgcc gccagatttt
    44041 ttggctttcg gcttgctgtg tccgttgctg gtttttaaag gttcgtcttt ggcgatggct
    44101 tgcgacattc ctgacaacgt cagggcagcc aaaccggctt tcagcacctg gcggcgcgaa
    44161 gtgagtgttt ttagtggttt aaaagtgctc atacggcctg agttgtaata ataaagttcc
    44221 agatgttata tcgtatcgcg taatccgtta cgacggtttg atgttgagaa ttgttttact
    44281 tttcatttca atagttgaca actccccatt atgccacttt ttcaaccgat tttcgtcaga
    44341 tattggctaa ttcgaccgtt cacgccataa tcagcctttt aacccgcaaa aaggtaacga
    44401 ataccatgga gatacgcgta tttcgccagg aagatttcga agaggtcatc accctttggg
    44461 agcgttgcga cttgctgcgt ccgtggaacg atccggaaat ggacatcgag cgtaagatga
    44521 accatgacgt cagtttgttt ttggtcgctg aggtaaacgg tgaagtggtc ggaacggtga
    44581 tgggcggtta tgacgggcat cgcgggtctg cttattatct tggcgtgcat ccagagtttc
    44641 gtgggcgtgg gattgccaat gcgttgctta atcggctgga gaaaaagctg attgctcgtg
    44701 gctgcccgaa aattcagatc aatgtgccgg aagataacga catggtgctc ggaatgtatg
    44761 aacgcctggg atatgaacac gccgacgtgc tgagtctggg taagcgtttg attgaagatg
    44821 aagagtactg agttccatcc tgtccattat gatgcgcacg gacgcctgcg tttacccttg
    44881 ctcttctggc ttgtgctatt gcttcaggcg cgaacctggg tgctgtttgt cattgccggt
    44941 gcgtcgcgcg agcagggtac tgcgctgcta aatctgtttt atcccgatca cgataatttc
    45001 tggctggggc tgattccagg cattccggcg gtgctggcgt ttttgttgag cggtcggcgg
    45061 gctacatttc ctcgcacttg gcatgtgctc tattttctgt tgctgttggc gcaggtggtt
    45121 ttactttgct ggcaaccgtg gctgtggctg aacggtgaat ccgttagcgg tatcggtctg
    45181 gcgctggtag tggcggatat tgtggcgtta atctggctgc tgaccaatcg acgtttacgc
    45241 gcttgtttta atgaagaaaa agaataacgg cactttttgg tgaatttgca ctccaagcaa
    45301 cgttattgaa taaccaaagg aagtgacatg aaatcgctgc gtttaatgtt atgcgctatg
    45361 ccgttgatgc tgaccggctg ttccacgatg tcgtcagtta actggtcggc cgctaacccg
    45421 tggaactggt ttggatcgtc caccaaagtg agcgagcagg gcgtgggtga attaacggcg
    45481 tccacaccac tgcaagaaca agccattgct gatgcgcttg atggcgatta tcgcctgcgc
    45541 agcggaatga aaaccgcgaa cggcaacgtg gtgcgctttt ttgaagtgat gaaaggcgac
    45601 aacgtggcga tggtgattaa cggcgatcaa ggcacgatca gtcgcattga cgtgctggat
    45661 agcgatattc ctgctgacac cggtgttaaa atcggtacac cgtttagcga cctttacagc
    45721 aaagcatttg gcaattgcca aaaagctgat ggtgatgata atcgtgccgt cgaatgtaaa
    45781 gccgaaggca gtcaacatat tagctaccag ttcagcgggg aatggcgtgg tcctgaaggg
    45841 ttaatgcctt cggacgatac cctgaaaaac tggaaagtca gtaaaattat ctggcggcgt
    45901 taatttgcgt ctgaacaaac cgccgccgcg aaaaaacacg taaaatagcg ctcaacaatg
    45961 ccacggattg cgtggcattc ttattttcag gaggaacaat gtctcaggtt cagagtggca
    46021 ttttgccaga acattgccgc gcggcgattt ggatcgaagc caacgtgaaa ggggaagttg
    46081 acgccctgcg tgcggccagt aaaacatttg ccgacaaact ggcaactttt gaagcgaaat
    46141 tcccggacgc gcatcttggt gcggtggttg cctttggtaa caacacctgg cgcgctctga
    46201 gcggcggcgt tggggcagaa gagctgaaag attttccggg ctacggtaaa ggccttgcgc
    46261 cgaccaccca gttcgatgtg ttgatccaca ttctttctct gcgtcacgac gtaaacttct
    46321 ctgtcgccca ggcggcgatg gaagcctttg gtgactgcat tgaagtgaaa gaagagatcc
    46381 acggcttccg ttgggtggaa gagcgtgacc tgagcggctt tgttgacggt acagaaaacc
    46441 cggcgggtga agagacgcgt cgcgaagtgg cggttatcaa agacggcgtg gatgccggcg
    46501 gcagctatgt gtttgtgcag cgttgggagc acaacctgaa gcagctcaac cggatgagcg
    46561 ttcacgatca ggagatgatg atcgggcgca ccaaagaggc caacgaagag attgacggcg
    46621 acgaacgtcc ggaaacctct cacctcaccc gcgttgatct gaaagaagat ggcaaagggc
    46681 tgaagattgt tcgccagagc ctgccgtacg gcaccgccag tggcactcac ggtctgtact
    46741 tctgcgccta ctgcgcgcgt ctgcataaca ttgagcagca actgctgagc atgtttggcg
    46801 ataccgatgg taagcgtgat gcgatgttgc gtttcaccaa accggtaacc ggcggctatt
    46861 acttcgcgcc gtcgctggac aagttaatgg cgttgtaagc gtttctgatt cgatgattaa
    46921 aggccagcca gtaacaaaac tgactggcct ttttattact tttgcttcaa cgccgcatac
    46981 acctgatcaa ctacccaacc ataagttgca atcggtaact gaccgctttc gaacatattg
    47041 ggattttttt gtggatcggc aactggcgaa tggggcttgt tgcttaacat cacaatcgcc
    47101 atatgattga ccggatcgat aacggttact gtcccggtcc agccagtgtg accgtaggtt
    47161 tgcgggcttg ccagcgtgcc aaacgtcggc gtcatggtgg cattaccatt cacgcgccag
    47221 ccgaggccaa aagtggcatc ttccttagag ctggtggtga acatcttcac cgtttccgca
    47281 ctgaacagct gcacatcgcc atagcccccg ccgttcagca tcgtttgcat taacaccgca
    47341 atatcgccgg tattggaaaa caaacctgcg tgcccggaaa cgccgcccat cgaataaaag
    47401 gctttttcat cgtgcacctg accccagaga gtggaggtgc ggatattcgg gaaatggatc
    47461 acgccatcgc gggtattgcc gttcaattcc gtggcggcaa tttgttgcgg tttaaagcct
    47521 ttcagcagcg ggttaaatac cgtatgtgtc aggccgagcg ggcgataaat cgactcttca
    47581 acatagcggt caagcggctg accggtaacc gactcgacga taaatccaag cagcatataa
    47641 tcgacatcgc tgtagatatg tttgctgccg ggctgatatt ccagcggcgt gcgcttgatc
    47701 atctccagcg tctggccttt atcctgggaa tataacgcgc ccgcgaccgc tttattcggg
    47761 tattgcggat ctgccgggaa accgccgctg tgatgcagca ggtcagaaat ccgcagggtg
    47821 tttttgcctt taatggtgtc atttggacta tcggcaaacc ccggaatata tttcgcaatc
    47881 cgatcgtcag gatgcagttt gccttcgctc atcagctttt gcagggcgaa gttcgtggcg
    47941 tacattttgg tgtttgaggc caggtcatac agcgtcccgg tggtggcttt gacaggctgt
    48001 tccatcaaca cgctgccatc gtactttttc gccgctcccc aggctttgcg atacacaatc
    48061 tgattatctt taatgatcag caggtttacg ccgggataac cgacatcaat ttgctggcta
    48121 atccagcgat ccatctggtt aagccgttcg acgttaaacc ctgctttctc tggcgagctt
    48181 tctgtcagaa cagggtattt tgcggcgctg acgctacaac taacagccag cagagaaaga
    48241 tagagcattg tccgtttcat gatatcagtc cagattgacg ttacggcagc caaagagcgt
    48301 ggtgaaaata aacccgcaaa cccatgccac cagaatcccg ccagcataaa ccgccatggc
    48361 cggaaggatg ccttgtgcgg aagtcatcag cggcagcgct accagaccag acggcccaaa
    48421 ggcgctgttt aagcccatcg gcagacccca tcaggctatc aggccgataa acaaaccgcc
    48481 cgccgcgccg cctaaacagg cggtaacaaa cggcttcatg cggggcaggg tgacaccgta
    48541 aatcagcggt tcaccaacgc ccagcaggcc gggaataatc gccccgcgta cctgactgcg
    48601 tagcgcactg tgcggttgcg cccgccagta gagtgccagc gccgcgccca cctggcccgc
    48661 gcctgccatt gaaaggatgg gaaataagct gttgaatccc tagctgtcca ttaacgcgag
    48721 gtaaacagga ataaagccct gatgcacgcc aaataccacg gcgatcagga acagcccggc
    48781 taaaaccgca caaccgaacg gattactgtt caggtgcata aacagccatg acataccttc
    48841 gaataaccag ccacccagcg gcataatgat caggtacgca agcgtagcgg tgatcagcag
    48901 ggtgattaac gaggtcagca gcatgtcgag atcgtccgac ataaagcggc gcaccatgcc
    48961 ttcaatgcgg gcgcaggccc acgcggcaat cagcacgccg ataatattgc cgcgcggatc
    49021 gatgggcaga ccaaagaaat cgtgaaaacc ggcgtagtca ccggtggttg cagcggggtt
    49081 ataaccgagc aaaaagagtg cggcaataat tgcgccattg acgcccgtgc cgccaaatgc
    49141 ctgggcggcg ttatagccca ccagaatcac caggaaagtg aacaaacctt tgctgaacac
    49201 cttcataaaa ttcagcgcgt cgggtagcgt gccctgagca tctgccggaa cgtgcatcac
    49261 cgtggcaatt aacgtcgcta tccccagcag cagaccggcg gcaataaaac cggggatcag
    49321 cggcgtgaag atggtggcga atttggcgag aaattgttgc acgccggagg tttgtttggc
    49381 ttttaactga cgtttgttct gggcggcgat ttctgcggca tcctgtaccg gtgcctcgcc
    49441 cagcagctcg ctcatcgctt ttgcggcacg atgcgcttta ccaggcccaa aaacgacctg
    49501 cacctggtcg ctggtcaaaa tgacgccctt cacgccttcc agcgttttga tattgggatc
    49561 aaccagtgaa ctgtcatgta cacccagacg caggcgcgtc atacagttac cacaactggc
    49621 gatatttccc agtccgccga cacgggtaag aatggtgttc agaagttcac tgctgatctc
    49681 tttggccatg ggttattcct tgtctaaaac ctgacgaata aagccgccgt gttgatccag
    49741 gcgtttttta gcttctgcgg catcgagatt tttcagcacc atcacaatgg ccgttttaca
    49801 gttgcgctcg caggcaatta acgccgcttc cgcttgttcg gcattacatc cggtggcgtt
    49861 tttaacgata ttgacctggc gcacatgcag tttttcgttg gtggcgacca catcgaccat
    49921 caggttgccg aacactttgc cggatttaat catcagcccg gtggaaagca tattgagcac
    49981 cagtttctgc gctgtgcctg ctttcattcg cgaagaacca gtgaccactt cggcacctac
    50041 aatcggtgta atggcaaact cggcggtggt ggaaacggca ctccccggat tacaggaaat
    50101 tcccactgtg cggcaaccga gctggcgggc gtattccagt ccggcaatca catacggcgt
    50161 acgaccgctg gcggcaatac cgaccaccac atcctgtgcc gttaaattta tatttttcag
    50221 atcgttaatg ccgccttctc ggctatcttc cgtgccttcc accgcgtgct gaatggcata
    50281 ttcgccgcca gcaatcaaac caaccaccag acccggtttc acgccgtagg tgggcggaca
    50341 ttcgctggca tccagaatcc ccagacgacc ggatgttccc gcaccgagat aaatcagacg
    50401 accgccgccg cttacctggg cgtggataac atcgatcgcc gcggcgatat ccggcaggac
    50461 gcgctcaacg gcaagcggta cggttttatc ttcatcgttg ataatccggc acatttccag
    50521 cgtcgatacg cggtcaattt cagccgaggc ggtgttcgag ccttcagtaa tcatcttttc
    50581 aagttgcatc ggtggtgacc ttactattta cgatcaaata attgcagatg tatggaatca
    50641 tatatcccga attgacagac ttaaaggaat cattgagtct acacttgcag agtgtgaaga
    50701 gcgtcataag aacttaagcg gaatttttgc tgccagcccg ggagagtgga agatgttgta
    50761 cctgacgaaa atacgcaatg cggaaagtga atttaccgag aacgagcaaa aaattgctga
    50821 ctttctgcgg gccaacgtga gtgagctgaa atccgtctct tcgcgcaaga tggcgaaaca
    50881 gctcggcatc agtcagtcga gcattgtgaa atttgcccag aagctcggcg cgcaaggatt
    50941 tactgaattg cgtatggcgt taattggcga atacagtgcc agccgggaga aaacgaatgc
    51001 cacggcgctg cacctgcaca gttcgattac cagtgatgat tcgctggaag tgattgcccg
    51061 caaactgaat cgcgaaaaag agctggcgct ggagcaaacc tgtgcgttgt tcgactacgc
    51121 tcggctgcaa aaaatcattg aggtcatcag taaagcaccg tttatccaga taacgggcct
    51181 gggcgggtcg gcgctggtgg ggcgcgatct gtcgttcaaa ctgatgaaaa ttggctatcg
    51241 cgtggcctat gaagccgaca cacacgtaca agccaccgtt tcccaggcgt tgaaaaaagg
    51301 cgatgtacaa atcgctatct cttacagcgg cagtaaaaaa gagattgtct tgtgcgcaga
    51361 agcggcacga aagcaggggg caacggtgat tgccattact tctctggctg actctccgct
    51421 gcggcggctg gcgcatttca cgctcgatac cgtttccggt gaaaccgaat ggcgtagctc
    51481 gtcgatgtct acccgcacgg cgcaaaattc tgttaccgat ttactgtttg ttggtcttgt
    51541 gcaactcaat gatgtggagt cattgaaaat gattcagcgc agcagtgagc taacacaacg
    51601 cctgaaatga tgcataaagc agcgactgga ttgagatttt cctgaattag tgagctgatc
    51661 cgcagcaata ttttgtttat cctgtatttt cagagggaat ggagtgtaac gctctgtatt
    51721 aacaaggaga gcattaaaat gggtaaactc acgggcaaga cagcactgat tacgggcgca
    51781 ttgcagggaa ttggcgaagg aattgccaga acttttgcgc gtcatggcgc aaacctaatc
    51841 ttgctggata tctcccctga gatcgaaaag ctggcggacg aactgtgtgg tcgtggtcat
    51901 cgctgtacgg cggttgtcgc cgatgtgcgt gacccggcgt cggtagccgc agctatcaaa
    51961 cgcgcgaagg aaaaagaagg gcgcattgat atcctggtga ataacgcagg cgtttgtcgt
    52021 ctgggcagtt tcctcgatat gagcgatgac gatcgcgatt tccatattga catcaatatt
    52081 aaaggcgtat ggaacgtcac gaaggcggtg ctgccggaga tgattgcccg caaagatggt
    52141 cgcattgtga tgatgtcttc agtcactggt gatatggtgg ccgatcctgg cgaaacggcg
    52201 tacgccttaa cgaaagcggc gattgttggc ctgacaaaat cgctggcggt ggagtacgcg
    52261 cagtctggta ttcgcgttaa cgccatttgc ccgggatacg tgcgcacacc aatggcggaa
    52321 agcattgccc gccagtcgaa cccggaagat ccagagtcgg tgctgactga aatggcgaaa
    52381 gcaatcccgc tgcgtcgcct cgccgatccg ctggaagtcg gcgaactggc ggccttcctc
    52441 gcatcggatg aatccagcta tttaaccggt acacagaatg tgattgatgg cggcagcaca
    52501 ctgccggaga cggttagcgt cggtatctga ttcacctctg tttcctccct gcatttgtgg
    52561 ggaggatttc gttttaaacc cggttcttat tcccttttca acttccaaat caccaaacgg
    52621 tatataaaac cgttactcct ttcacgtccg ttataaatat gatggctatt agaaagtcat
    52681 taaatttata agggtgcgca atggccgtta acttactgaa aaagaactca ctcgcgctgg
    52741 tcgcttctct gctgctggcg ggccatgtac aggcaacgga actgctgaac agttcttatg
    52801 acgtctcccg cgagctgttt gccgccctga acccgccgtt tgagcaacaa tggaccaaag
    52861 aaaacggcgg cgacaaactg acaataaaac aatctcatgc cgggtcatca aaacaggcgc
    52921 tggcaatttt gcagggctta aaagcagacg ttgtcactta taaccaggtg actgacgtac
    52981 aaatcctgca tgacaaaggc aagctgatcc cggcggactg gcaaacgcgc ctgccaaata
    53041 atagctcgcc attctactcc accatgggct tcctggtgcg taagggcaac ccgaagaata
    53101 tccacgactg gaacgacctg gtgcgctccg atgtgaagct gattttcccg aacccgaaaa
    53161 cgtcgggtaa cgcgcgttac acctatctgg cggcatgggg cgcagcggat aaagctgacg
    53221 gtggcgataa agccaaaacc gaacagttta tgacccagtt cctgaaaaac gttgaagtgt
    53281 tcgatactgg cggtcgtggc gcgaccacca cttttgccga gcgcggcctg ggcgatgtgc
    53341 tgatcagctt cgagtcggaa gtgaacaaca tccgtaaaca gtatgaagcg cagggttttg
    53401 aagtggtgat tccgaaaacc aacattctgg cggaattccc ggtggcgtgg gttgataaaa
    53461 acgtgcaggc caacggtacg gaaaaagccg ccaaagccta ccttaactgg ctctacagcc
    53521 cgcaggcgca aaccatcatc accgactatt actaccgcgt gaataacccg gaagtcatgg
    53581 ataaactgaa agacaaattc ccgcagaccg agctgttccg cgtggaagac aaatttggct
    53641 cctggccgga agtgatgaaa acccacttca ccagcggcgg cgagttagac aagctgttag
    53701 cggcggggcg taattaatgt ttgcagtttc atcaagacgc gtgctgccgg gctttacctt
    53761 aagcctcggc accagtctgc tgtttgtgtg cctgattttg ctgctgccgc tctccgcgct
    53821 ggtgatgcag ctggctcaga tgagctgggc gcagtactgg gaggtgatca ccaacccgca
    53881 ggtagtagcg gcctacaaag taacgctgct gtcagcgttt gtggcatcga tttttaacgg
    53941 cgtttttggc ctgctgatgg cgtggatctt aacccgctat cgctttccgg gccgcacgct
    54001 gcttgatgcg ctgatggatt taccctttgc gctgccaacg gcagtcgccg gtttaacgct
    54061 agcctcgctc ttttctgtaa acggttttta cggtgaatgg ctggcgaagt ttgatatcaa
    54121 agtcacctat acctggctgg ggattgcggt ggcgatggcc tttaccagca ttccgtttgt
    54181 ggtacgtacc gtgcagccgg tgctggaaga gttaggcccg gaatatgaag aagcggcaga
    54241 aacgcttggc gcaacgcgct ggcagagttt ctgcaaagtg gtgctgccgg aactttctcc
    54301 ggcgctggtg gcgggcgtgg cgctgtcgtt tacccgtagt cttggtgaat ttggcgcggt
    54361 gatttttatc gccggaaata tcgcgtggaa gacggaagtg acgtcgctga tgatttttgt
    54421 gcgcttacag gagtttgatt acccggcagc gagcgcgatt gcttcggtga tcctcgcggc
    54481 atctctgctg ctgctgttct caattaacac tctgcaaagt cgctttggtc ggcgtgtggt
    54541 aggtcattaa tggcggaagt tacccaattg aagcgttatg acgcgcgccc gattaactgg
    54601 ggcaaatggt ttctgattgg catcgggatg ctggtatcgg cgttcatcct gctggtgccg
    54661 atgatttata tcttcgtaca ggcgttcagc aaagggctga tgccggtttt acagaatctg
    54721 gccgatccgg acatgctgca cgccatctgg ctgacggtga tgatcgcgct gattgccgta
    54781 ccggtaaacc tggtgttcgg cattctgctg gcctggctgg tgacgcgctt taacttccct
    54841 ggacgccagt tactgctgac gctactggac attccgtttg ccgtatcgcc ggtggttgcc
    54901 ggtctggtgt atctgctgtt ctacggctct aacggcccgc tgggcggctg gctggatgaa
    54961 cataatctgc aaattatgtt ctcctggccc ggaatggtgc tggtcaccat ctttgtgacg
    55021 tgtccgtttg tggtgcgcga gctggtgccg gtgatgttaa gccagggcag ccaggaagac
    55081 gaagcggcga ttttgcttgg cgcgtctggc tggcagatgt ttcgccgcgt cacattgccg
    55141 aacattcgct gggcgctgct ttatggcgtg gtgttgacca acgcccgcgc aattggcgag
    55201 tttggcgcgg tgtcggtggt ttccggctcg attcgcggcg aaaccctgtc gctgccgtta
    55261 cagattgaat tgctggagca ggactacaac accgtcggct cctttaccgc tgcggcactg
    55321 ttaacgctga tggcgattat caccctgttt ttaaaaagta tgttgcagtg gcgcctggag
    55381 aatcaggaaa aacgcgcaca gcaggaggaa catcatgagc attgagattg ccaatattaa
    55441 gaagtcgttt ggtcgcaccc aggtgctgaa cgatatctca ctggatattc cttcaggtca
    55501 gatggtcgcg ttgctggggc cgtccggttc cgggaaaacc acgctgctgc gcattatcgc
    55561 cgggctggag catcaaacca gcgggcatat tcgcttccac ggcaccgacg tcagccgcct
    55621 gcacgcgcgc gatcgcaagg tcggtttcgt gttccagcat tacgcgctgt tccgccatat
    55681 gacggtattc gacaatatcg cttttggcct gacggtgctg ccgcgtcgcg atcgcccgaa
    55741 tgccgcagcc atcaaagcga aagtgacaaa attgctggag atggtgcagc ttgctcatct
    55801 ggcggatcgt tatccggcac agctttccgg cggccagaaa cagcgcgtgg cgctggcgcg
    55861 cgcgctggct gtagaaccgc aaattctgct gcttgatgaa ccgtttggcg cgctggatgc
    55921 gcaggtgcgt aaagagctgc gtcgctggct gcgtcaactg catgaagagc tgaaattcac
    55981 cagcgtgttc gtgacccacg accaggaaga agcgacggaa gtagctgatc gtgtagtggt
    56041 gatgagccag ggcaatattg aacaggctga tgcgccggag caggtatggc gcgaaccagc
    56101 gacccgtttt gtgctcgaat ttatgggcga agtgaaccgc ctgcagggaa ccattcgcgg
    56161 cgggcagttc catgttggcg cacatcgctg gccgctgggc tacacacctg cttatcaggg
    56221 gccggtggat ctcttcctgc gcccgtggga agtggatatc agccgccgta ccagtctcga
    56281 ttcgccgctg ccggtacagg tactggaagc cagcccgaaa ggtcactaca cccaattagt
    56341 ggtgcagccg ctggggtggt acaacgaacc gctgacggtc gtgatgcatg gcgacgatgc
    56401 cccgcagcgt ggcgagcgtt tattcgttgg tctgcaacat gcgcggctgt ataacggcga
    56461 cgagcgtatc gaaacccgcg atgaggaact tgctctcgca caaagcgcct gataggttga
    56521 gtgaatgtta aacgcccgga ggcgcttccc gcgatccggg ctttttaatg gcaaggtttg
    56581 taacctgtag acctgataag acgcgcaagc gtcgcatcag gcaacaccac gtatggatag
    56641 agatcgtgag tacattagaa caaacaatag gcaatacgcc tctggtgaag ttgcagcgaa
    56701 tggggccgga taacggcagt gaagtgtggt taaaactgga aggcaataac ccggcaggtt
    56761 cggtgaaaga tcgtgcggca ctttcgatga tcgtcgaggc ggaaaagcgc ggggaaatta
    56821 aaccgggtga tgtcttaatc gaagccacca gtggtaacac cggcattgcg ctggcaatga
    56881 ttgccgcgct gaaaggctat cgcatgaaat tgctgatgcc cgacaacatg agccaggaac
    56941 gccgtgcggc gatgcgtgct tatggtgcgg aactgattct tgtcaccaaa gagcagggca
    57001 tggaaggtgc gcgcgatctg gcgctggaga tggcgaatcg tggcgaagga aagctgctcg
    57061 atcagttcaa taatcccgat aacccttacg cccattacac cactaccggg ccggaaatct
    57121 ggcagcaaac cggcgggcgc attacccatt ttgtctccag catgggaaca accggcacta
    57181 tcaccggcgt ttcacgcttt atgcgcgaac aatccaaacc agtaacgatt gttggcctgc
    57241 agccggaaga aggcagcagt attcc
//
LOCUS       DAJVYM010000009        55341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVYM010000009 DAJVYM010000000
VERSION     DAJVYM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.009"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OQS60_000516"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OQS60_000516"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS2386373.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OQS60_000517"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OQS60_000517"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS2386374.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OQS60_000518"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OQS60_000518"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS2386375.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OQS60_000519"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OQS60_000519"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS2386376.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OQS60_000520"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OQS60_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS2386377.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OQS60_000521"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OQS60_000521"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS2386378.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OQS60_000522"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OQS60_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS2386379.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OQS60_000523"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OQS60_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS2386380.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OQS60_000524"
     CDS             6537..6773
                     /locus_tag="OQS60_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS2386381.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OQS60_000525"
     CDS             6776..7633
                     /locus_tag="OQS60_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS2386382.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OQS60_000526"
     CDS             7654..9645
                     /locus_tag="OQS60_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS2386383.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OQS60_000527"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OQS60_000527"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS2386384.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OQS60_000528"
     CDS             complement(10248..11144)
                     /locus_tag="OQS60_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS2386385.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OQS60_000529"
     CDS             complement(11193..11432)
                     /locus_tag="OQS60_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS2386386.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OQS60_000530"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OQS60_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS2386387.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OQS60_000531"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OQS60_000531"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS2386388.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OQS60_000532"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OQS60_000532"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS2386389.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OQS60_000533"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OQS60_000533"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS2386390.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OQS60_000534"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OQS60_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OQS60_000535"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OQS60_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS2386391.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OQS60_000536"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OQS60_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS2386392.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OQS60_000537"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OQS60_000537"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS2386393.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OQS60_000538"
     CDS             complement(21570..22739)
                     /locus_tag="OQS60_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2386394.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OQS60_000539"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OQS60_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS2386395.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OQS60_000540"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OQS60_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS2386396.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OQS60_000541"
     CDS             complement(24315..25247)
                     /locus_tag="OQS60_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2386397.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OQS60_000542"
     CDS             25360..26571
                     /locus_tag="OQS60_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS2386398.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OQS60_000543"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OQS60_000543"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS2386399.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OQS60_000544"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OQS60_000544"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS2386400.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OQS60_000545"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OQS60_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS2386401.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OQS60_000546"
     CDS             complement(30320..31576)
                     /locus_tag="OQS60_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS2386402.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OQS60_000547"
     tRNA            31884..31960
                     /locus_tag="OQS60_000547"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OQS60_000548"
     tRNA            31965..32041
                     /locus_tag="OQS60_000548"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OQS60_000549"
     CDS             32149..32454
                     /locus_tag="OQS60_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS2386403.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OQS60_000550"
     CDS             32580..34184
                     /locus_tag="OQS60_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS2386404.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OQS60_000551"
     CDS             complement(34196..35008)
                     /locus_tag="OQS60_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386405.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OQS60_000552"
     CDS             complement(35012..35797)
                     /locus_tag="OQS60_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS2386406.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OQS60_000553"
     CDS             complement(35794..36462)
                     /locus_tag="OQS60_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS2386407.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OQS60_000554"
     CDS             complement(36526..37164)
                     /locus_tag="OQS60_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS2386408.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="OQS60_000555"
     CDS             complement(37177..39285)
                     /locus_tag="OQS60_000555"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS2386409.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="OQS60_000556"
     CDS             complement(39306..39932)
                     /locus_tag="OQS60_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS2386410.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OQS60_000557"
     CDS             complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OQS60_000557"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS2386411.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41154..42566
                     /gene="pykF"
                     /locus_tag="OQS60_000558"
     CDS             41154..42566
                     /gene="pykF"
                     /locus_tag="OQS60_000558"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS2386412.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42876..43112
                     /gene="lpp"
                     /locus_tag="OQS60_000559"
     CDS             42876..43112
                     /gene="lpp"
                     /locus_tag="OQS60_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS2386413.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OQS60_000560"
     CDS             complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OQS60_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS2386414.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OQS60_000561"
     CDS             complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OQS60_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS2386415.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OQS60_000562"
     CDS             complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OQS60_000562"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS2386416.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OQS60_000563"
     CDS             complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OQS60_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS2386417.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OQS60_000564"
     CDS             complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OQS60_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS2386418.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OQS60_000565"
     CDS             complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OQS60_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS2386419.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OQS60_000566"
     CDS             complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OQS60_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS2386420.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50388..50576)
                     /locus_tag="OQS60_000567"
     CDS             complement(50388..50576)
                     /locus_tag="OQS60_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS2386421.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OQS60_000568"
     CDS             complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OQS60_000568"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS2386422.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51083..54139)
                     /locus_tag="OQS60_000569"
     CDS             complement(51083..54139)
                     /locus_tag="OQS60_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS2386423.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54528..>55341
                     /gene="ydiK"
                     /locus_tag="OQS60_000570"
     CDS             54528..>55341
                     /gene="ydiK"
                     /locus_tag="OQS60_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS2386424.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYW"
BASE COUNT        13347 a        14557 c        13533 g        13904 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgca gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaaa gcaagaaatt ataagtgaac tgatatttat tatcaattga
    40201 aataaattta acttaagcga caattatttg tgacgttcat tgcagaaaat aaaaataata
    40261 ttatcgttat agattaaaat acataccgca attcgtataa tttcagcagt tgttatttag
    40321 aatgattaac tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc
    40381 tgaatggtca ttttaccagt gcgttaatga cggtttccat cgcctgcttt tccagctcac
    40441 tgcgctgaat gcgtttattg gctaatgcag tacgtaatac gcctgcgata acaatatgct
    40501 ttggttcctg ccccagatcg cgcatttcca gcacaacttt accgacaact ctgcacattt
    40561 ctcggtacag ttcatcctct tttgtccgat ttcccattct ttatacctat ttatcatgcc
    40621 aactatcagc atatatcaat ctaaccaatt aaacaaaaat caaacaaaat cagacaaata
    40681 acgcgataaa ttattttaac tgtagcaatt gagcgatgat atatttatac accggatgaa
    40741 ctttcactta tcctcacact gacaacttcg gcaccagacg ttgcgcaaac agcgaagttt
    40801 ttgcgtaacc ttttccctgg aacgttaaat ctttgataac aatttattgt ctaacaagtt
    40861 gtatattttt tgaaacgctg tttttgtttt ccttttggat taatttcagc gtataatgcg
    40921 cgccaattga ctcttgaatg gtttcagcac tttggactgt agaactcaac gactcaaaaa
    40981 caggcactca cgttgggctg agacacaagc acacattcct ctgcacgctt tttcgatgtc
    41041 acctatcctt agagcaaggc accaccactt tcgtaatacc ggattcgctt tccggcagtg
    41101 cgcccagaaa gcaagtttct cccatccttc tcaacttaaa gactaagact gtcatgaaaa
    41161 agaccaaaat tgtttgcacc atcggaccga aaaccgaatc tgaagagatg ttagctaaaa
    41221 tgctggacgc tggcatgaac gttatgcgtc tgaacttctc tcatggtgac tatgcagaac
    41281 acggtcagcg cattcagaat ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta
    41341 tcctgcttga taccaaaggt ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg
    41401 tttctctgaa agcgggtcag acctttactt tcaccactga taaatctgtt atcggcaaca
    41461 gcgaaatggt tgcggtaacg tatgaaggtt tcactactga cctgtctgtt ggcaacaccg
    41521 tactggttga cgatggtctg atcggtatgg aagttactgc cattgaaggt aacaaagtta
    41581 tctgtaaagt gctgaacaac ggcgacctgg gcgaaaacaa aggtgtgaac ctgcctggcg
    41641 tttccattgc cctgccagca ctggctgaaa aagacaaaca ggacctgatc tttggttgcg
    41701 aacaaggcgt agactttgtt gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa
    41761 tccgtgagca cctgaaagcg cacggcggcg aaaacatcca catcatctcc aaaatcgaaa
    41821 accaggaagg cctcaacaac ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg
    41881 cgcgtggcga cctgggtgta gaaattccgg tagaagaagt tatcttcgcc cagaagatga
    41941 tgatcgaaaa atgtatccgt gcacgtaaag tcgttatcac tgcgacccag atgctggatt
    42001 ccatgatcaa aaacccacgc ccgactcgcg cagaagccgg tgacgttgca aacgccatcc
    42061 tcgacggtac tgacgcagtg atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg
    42121 aagcggtttc tatcatggcg accatctgcg aacgtaccga ccgcgtgatg aacagccgtc
    42181 tcgagttcaa caatgacaac cgtaaactgc gcattaccga agcggtatgc cgtggtgccg
    42241 ttgaaactgc tgaaaaactg gatgctccgc tgatcgtggt tgctacccag ggcggtaaat
    42301 ctgctcgcgc agtacgtaaa tacttcccgg atgccaccat actggcactg accaccaacg
    42361 aaaaaacggc tcatcagttg gtactgagca aaggcgttgt gccgcagctg gttaaagaga
    42421 tcacttctac tgatgatttc taccgtctgg gtaaagaact ggctctgcag agcggtctgg
    42481 cacacaaagg tgacgtcgta gttatggttt ctggtgcact ggtaccgagc ggcactacta
    42541 acaccgcatc tgttcacgtc ctgtaatatt gcttttgtga attaatttgt atatcgaagc
    42601 gccctgatgg gcgctttttt atttaatcga taaccagaag caataaaaaa tcaaatcgga
    42661 tttcactata taatctcact ttatctaaga tgaatccgat ggaagcatcc tgttttctct
    42721 cagttttttt atctaaaacc cagcgttcga tgcttctttg agcgaacgat caaaaataag
    42781 tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac ttgtaacgct
    42841 acatggagat taactcaatc tagagggtat taataatgaa agctactaaa ctggtactgg
    42901 gcgcggtaat cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc
    42961 agctgtcttc tgacgttcag actctgaacg ctaaagttga ccagctgagc aacgacgtga
    43021 acgcaatgcg ttccgacgtt caggctgcta aagatgacgc agctcgtgct aaccagcgtc
    43081 tggacaacat ggctactaaa taccgcaagt aatagtacct gtgaagtgaa aaatggcgca
    43141 cattgtgcgc catttttttg tctgctattt accactactg cgtcgcgcgt aacatattcc
    43201 cttgctctgg ttcaccattc tgcgctgact ctactgaagg cgcattgctg gctgcgggag
    43261 ttgctccact gctcaccgaa accggatacc ctgcccgacg atacaacgct ttatcgacta
    43321 acttctgatc tacagcctta ttgtctttaa attgcgtaaa gcctgctggc agtgtgtatg
    43381 gcattgtctg aacgttttgc tgttcttctg ctgatagtgg tcgatgtact tcaacataac
    43441 gcatcccgtt aggttccacg gaatatttca ccggttcgtt gatcactttc accggcgttc
    43501 ccgtccgcac gctggagaac aaggctttaa tatccggtgc attcatgcga atacaccctg
    43561 aactgacgcg caaaccaacg ctgtccggcg cactggtgcc atgaatgagg tattcgccat
    43621 taccatgcgc gaggcgcagt gcgtaacgtc ctagcgggtt atttggtccg gcaggaacga
    43681 ctggcggtaa tttaatgcca cgctccagcg aacgctgacg aatgcctgcc gtaggcgtcc
    43741 aggttgggtt agggattttc tgcccaacac gcgtttccat caccggcgtt tccagcccct
    43801 gcaatccaat acctattgga taaacctgca caatattttc tcccggcgga taataataaa
    43861 ggcgcagctc tgcaaggtta acgataatcc cctgacgcgg tgcatcaggt aataacagtt
    43921 gtgaaggaat agttatcgtc gtaccaggtt ttggcaccgg gacgatagtg ttattggctt
    43981 caaggatcaa cattgccgca gtatcaaaac gtcgggcaat agcctgaagg tttttatccc
    44041 cttcttgcac cgtatacgtt tgattttgcc caaccagtcg gcttccggtt ggtggtagcg
    44101 gataatcaac cgcccaggca gcctggatgg cgctaaaagc gccgataagc gtgagtgtaa
    44161 gcaaagacgc gcgtttcatt gtaaacctcc tgtatttgcc ggagactcac gctgaaacgt
    44221 cggatggcgc ttatgttcac ctgaaaccaa aacactcctg tgcaggtcag tgtaaacatt
    44281 gaccatccgg caatgtgagc caaccggatg aaagctgtcc ctttagttta gctaagtgca
    44341 gcggctttgg cgcgaattgc gcgaatcatc gcttccagac cttgtgaacg agatggggtg
    44401 agatgttggg tgagtgccat tttttcaaac cacggacgca catcgaaact gacaatatcc
    44461 tgcggcgtca tctgatcgta gagaataaag actaccgcaa taagcccttt cacaatcgcc
    44521 gcatcgctgt cgccctgcaa ttcaataatt ccgtgcgcat tctggcgcat gacaatccac
    44581 acctgactct ggcagccctg aatgctattt tgtgggcttc ggtcttcgtc gcgtaattca
    44641 gtcagacgct ggcccagctc aataatgtag agatatttct cttcccagtt ggcgcagcgt
    44701 aaaaaattac gcagcaattt ttctttatcc ggcaataaag ccatagtgcc tccctgttat
    44761 cccagcaaac ggtgaatacg ttgcaggccg gtcaccagac gatccacttc ttcatgggtg
    44821 ttatacatgg ccagcgacgc ccgacacatc gcagggacgt tgtaataggc cattaatggc
    44881 atagcgcagt gatgtccggt acgcacagca atgccgtaat tatcgagaaa actgccaaca
    44941 tcataggcgt ggtgtttacc gagattaaaa gcaataacgc caagcctgtt ttgtgggcca
    45001 tagagagtga gatccggtac agattccagc tgtgatagcg cataatgcat cagattctgt
    45061 tcatactcgg ctatgttatt aagccccagc gccgaaacat actccagcgc cgcgccaaga
    45121 ccaataatgc ccccggtatt gggtgtaccg gcttcaaacc gccatggtgc tttggtccag
    45181 gtagtgcctt cactcaggct gacggtggcg atcatagaac cgcccccttc ccacggcggc
    45241 atctcctgca acaaggcttc tttcacataa agaatgccaa ttccggtggg gccatacagt
    45301 ttatgcccgg agaacacgta aaagtcgcaa tccagcgcct gaacatccac cggatgatgc
    45361 atcaccgcct gagcgccatc caccagcact tttgcgccat gctggtgcgc aagcgtgatt
    45421 atttccgcca gcggattttc tgttcccagc acgttggaga catgagtaat tgccagcagg
    45481 cgagttttct catcaaacag cgtaggcagc gtctccagtt gcagcgtacc gtcggggttg
    45541 agagggatca cacgcagctc tgcgccaacg cgtgcgcaaa gcatctgcca gggaacaatg
    45601 ttagcgtggt gctccatctg actgatgatg atgttatcgc ccgcccgcac gttgctgttg
    45661 ccccaactat tggcgaccag attgatccct tccgtcgtgc cgcggacgaa caccagctct
    45721 tccgccgaac gggcattaat aaacagcgat gcccgcttgc gcacgttctc cattttctcg
    45781 gtcgcctggg cgcttaaggt atgaataccg cgatgcaccg ccgcgtagcc gtgacgataa
    45841 aactcagcct cggcgtcaat cacctggctt ggtttctgcg cactggcggc gctatcgaga
    45901 taagccagcg gcaaaccgtt cacctcacga gaaagcaccg gaaagtcggc ccgcactttg
    45961 tcgacggaaa aagtcatctt gcacctcctg gcagccgttg accgattcgg gccagcacct
    46021 gctgtttaag cccctcatca cgcagtgctt ccgtcagttc ggcagcaaag gcgtaaatga
    46081 tcatctgctg ggcatcctgc tgattgatcc cgcgcgagcg cagatagaac atctgttcat
    46141 catcaatacg ccccaccgtc gcgccgtggc tgcatttcac atcatctgca tagatttcca
    46201 gctgcggttt tgtatccact tccgccattt tgcccatcag cagattattg ttggtcatct
    46261 gaccatccgt tttgatggcg tgctgcgcga cgttgatcaa accgttaaat accgcgcggc
    46321 ctttgtcgct gacgatagtt ttgtgcaact gtcggctgtt acaaaaacct ttattgtgtt
    46381 ccagccaggt acgggtatca cacacctcgt ttttcaccgg catcgccagg ctattgatcc
    46441 gcagcgtgct gttttcgcca ttgagttgcg tactggtgtt gtgtcgtaac actgcgccac
    46501 ccagcaggaa actatggcta aatgcggtgg catcctcagc cagcaacaaa tcgttatgag
    46561 caaagtggtg actgagcggg ttttcaaacg ccagcttgat atgctgcaag tgggcgttcg
    46621 ctgcgacatt gatagtgaac cgtgccccgg taaaatgacg agcatcatta aggctgacaa
    46681 aatgttcgat caccgttgct tcggcacctt ccgccagatc cagatgatgc cggtaatggg
    46741 cagtgttcac ctcttcacct gccacgccct gggtgatatg cattaacagc aatggctttg
    46801 ccggccgttg accgcgcttc acggcgatat gcgtcacgct ttgtgccagg ctttccgtca
    46861 aatgcagaaa cacttccgcc tgaatagcgt cgggtaaccc ctgacggtcg tcgttaatgc
    46921 tcacttcata tccgctgcct tcaattgcat cgctcagcgc gggcacgtaa cgtccatcga
    46981 caaacaccag ccgcacggcg tctaacgtta acgctaaggc atcacgctgc tgtggggata
    47041 tatctcccgc aatgctgaca aactgactat tggtcagccc ttccagcggc gtatatttcc
    47101 agttttcatg tttacgtgtc ggcagtccgg tacgcagcaa ttgttgtaaa tgctgctgtg
    47161 cttgcggtga gcgttttgtc ccttcagctt caaacaagtg atgccactgt tgcagcgcat
    47221 tactgctgtt cggtaagcca gccataaccc tgctcctcca gttgtttgac caacgtgaaa
    47281 tcgccggatt tcacaattcg tccctgatat agcacatgga cgtaatcagg cttgatgtag
    47341 tcgagaatgc gttggtagtg cgtaacaatg atgaatgagc gcttgccgtc acgcagcgag
    47401 ttcacgccat cggcaaccac ttttaatgcg tcaatatcca gcccggagtc cgattcatca
    47461 agaatgcata actccggttc cagcaccgcc atttgcaaaa tatcgttgcg ctttttctcg
    47521 ccgccggaaa aaccaacgtt taccgaacgg gttaataaat cttccggcat cttcaggaga
    47581 gcgattttct cttccatcaa atcctgaaaa tcaaagcggt cgagcgtttc ctggccgcga
    47641 tagctgcgca ccgcattaag tgccgtttgc aggaaaaact ggttactgac gccgggaatc
    47701 tccaccggat actggaaggc cataaagatg ccttcgcctg cacgatcttc cggcgacagc
    47761 gcaagcaaat ctttgccttt gaactcaacc gtgccgcccg tcacttcata atcttctcgc
    47821 ccggcaagcg ttgccgataa ggtacttttg cccgaaccgt ttggccccat aatggcgtga
    47881 acttcgccgg gacgaacgtc gaggcttaat ccacgcagga tagctttatc ttccacgctg
    47941 acgtgtaaat ctttaatact taacatgttt attccttatc cgacgctgtg ttcaagactg
    48001 atggcgagga gtttttgtgc ttcaacggca aattccagcg gcagttctga gaacacgtct
    48061 ttgcagaaac cgttaacaat catcgagatg gcgtcttctt cgctaatccc gcgttgcagg
    48121 cagtaaaaca gttgatcttc accaatacgt gatgtcgttg cctcgtgctc cagttgtgcg
    48181 ctgttattgc gacattcgac atacgggaag gtatgcgccc cacaattagc gccaatcagc
    48241 attgagtcgc actgagtgaa attgcgcgca ttggttgccg tcggcatgat tttcactaag
    48301 ccgcgataac tgttctgact atgtccggca gagatccctt tcgagataat ggtcgatttg
    48361 gtgtttttac cgatgtggat catcttggtg ccggtatccg cttgctgatg accgctggtc
    48421 agcgccactg agtaaaactc accaatggag ttatcgccgc gcaaaatgca gctgggatat
    48481 ttccacgtaa tcgctgaccc ggtttctgat tgcgtccatg acattttgct gttttcgcct
    48541 tcgcacaaag cacgcttggt gacgaaattg agaataccgc cggtgttgtt atcgccaggg
    48601 aaccagtttt gtaccgtgga atatttcacc tcggcgtttt tatggatgat gacttccacc
    48661 accgccgcgt gtaactgata gctgtcacgc accggagcgg aacagccttc aatgtagctg
    48721 acgtagctgt cttcgtcggc caccagaatg gtgcgctcaa actgcccggt tttttccgcg
    48781 ttaatgcgaa aataggtgga aagttccatc gggcagcgca cgcctttagg cacataaata
    48841 aacgtaccgt cagaggctac cgccgcatta agcgcggcaa agaagttgtc atttcccggc
    48901 accacagtgc cgagatattt acgcaccagt tccggatgat cgtggatcgc ctcaccaaag
    48961 gaacagaaaa taattccctg ctccgccagt ttttcgcgat aagtagtggc aaccgaaaca
    49021 gagtcgaaaa tggcatccac cgccacctct ttgccttccc gcacgggaac gcccaactgc
    49081 tcaaacgccg cctccacctc tttacttaaa aaggcgttcg cgccagtttg ctgcaccgcg
    49141 ccaggttcag acgcgcaagt gtcgtcacaa ttaccgcacg atggtgctga gtagtaactg
    49201 taatcctgat aattcagctt gtcgtagtgc gctttcaacc agtgcggttc ttccatctcc
    49261 agccatgcgc gataggcgtt tagacgaaac tccagcatcc actccggctc attacgcttc
    49321 gccgaaattg cgcgcaccac ctcttcgttt atcccctttg ccagctcatc ggtggctaac
    49381 tgggtgaaga atccttcttt ataattcagc gggccgccgg tccaggtttt gacatcgtca
    49441 gttgcttcag tattacgaga catagtaccg cctatacccc aaagctttcg ccacagccac
    49501 attcattctg ggctttaggg ttgtgaaatt tgaatatctg attaagtcct tcacgaacga
    49561 aatcgacttc cgtgccatca ataaacggca tcgcttgcag cgggacaaac agcttcgcgc
    49621 cgtcgtgttc aaacagcaga tcgtctttgt ccggctcgct aacactgtcg agcacatagc
    49681 caaagcccgc gcagcccgtt tgcttcacgc ctaagcgcac gccgaccata cccggctgct
    49741 ttgccaccag ctcacggatg tgtatcgccg ctgcgggtgt cagcgttaag ccttgccagg
    49801 cgaaatcttg tgggttaaag gttcctgaat gcatgtccat cgatttacct cacttcatcg
    49861 ctttcagcgt ataacagcat gctagtgata atgattatca gttcaaccca gcaaacgcag
    49921 gggctttacc gtaaaacatg ctttttgcct gcttttaata agcatagacc ctgatgtgtg
    49981 ggttaacagg cacgctaaga attaggtatc tcattgttag ataatggtta ttatctaagg
    50041 agcattaagg ctgtgatgaa aaagaaaaag ttgtattgaa aatgactatt taagagatag
    50101 gtaaaaaagt gcagcgttca gaaataagaa aacccttaag tctgtgcgac acaggcttaa
    50161 gggtttctac cccatccggc gcttatctcc ggcactctca gtggcttagc tcttgaaggg
    50221 gcgataagaa taatctcata aagctaaccc gccgttttaa cacaaactgc gattagtatt
    50281 atttttgaac aatatcaggc ggtagataag cagtattaag aaggtcatcg aacctggacg
    50341 gaggttaatc caggtcgatt tggcgaactt gcggcattaa gtcaggatca atgcacgccc
    50401 agacgccagg caaagtagat ttcttctttt aattcagtag aagagagagt aagcaggtca
    50461 gcaaattcaa gttctagttg tttcagacgt ttgagatatt gggcaggtga aagattgctc
    50521 tggtcacggc gtaaaaattc aatggccagc tgggtgggat caagttgagt agacatagca
    50581 tcctcgcttt tagacaagac gtgcacagta taccaccgtt tactgtgcag ataatgacca
    50641 aaagcaatat gcgtcacact tttctggtga caacgtcaca aaatggcggt cgtcaatcgt
    50701 gacgaacagc acaaacgccc tttctcatcg aagatttcaa tctgccagac ctggtgacgc
    50761 gaaccgagat gcaacggttt acatatgccg cgcacccgcc cttctcgtgc cgagcggacg
    50821 tgattagcat tgatttccag accaaccact ttttgctcac cttcggtaca taaataaccg
    50881 gcaacggaac cgatactttc ggccagtacc acggatgctc ctccatgcag caacccgaaa
    50941 ggctgctttg tccgcgagtc tactggcatt gtcgcttcaa gggtgtcatc accaatatgt
    51001 tcaaagcgaa tatccaggaa ccccaccatg tttccttcac ccatagcatt cagtgcttcc
    51061 agggtgattt tccgtttcca tatcatttaa taatctccag taaagcctgc acaggatggc
    51121 gtacccccgt gccttcaacc cgttttacct ggctacggca ggaatatccg gtcgccagac
    51181 agcggttacg cggcagtcgc tgcatagcct gatgccagga taactcatag atcccgagcg
    51241 aattttcatg gtttttcgct tcatgtccgt aagtccctgc catgccgcag caacccacgc
    51301 tgacattttc cagtttcgcg ccaaaacggg caaatatcgc ggcccattgt gctggcgcgc
    51361 ccggcaaagc ggtaacctcg gtacagtgac caaagaaata ccatgattca ccgctgactg
    51421 tagccactgg atgtgactca agtgcgcttg ccagccattc attcgcaagt aagacgttaa
    51481 actcgccacg ttcctcgccc agcgccagtt tatattcatc gcgataacaa agtaccagcg
    51541 ccggatcgac gcccaccatt ggcatcccaa gcttagccat acggttgagg aaatccgccg
    51601 tctttttcgc cgtcttcgca aaacgattaa gaaagccttt gatatgctgg gctttgccat
    51661 ttggcgaaaa tggcagtaac acaggctgga aacctaattt ttcaaccaga cggacaaaat
    51721 ccgccaccac ttgcgcatcg taatagctgg taaaggggtc ctgcaccacc aacaccgtgc
    51781 gcgctttctg ctctgcattg agagcttcaa gctgttccag cgtcatgttt gccgagcgat
    51841 gccccaccat ttgttgttgt agcgagggga ccgacagcag tggcaaatca accatgccga
    51901 tatgtttttc cgagagtttg cgcaccagcg gctggttaat gaagaagtta aaggttttcg
    51961 gcgcgcgtgc catcagcggt gcgtagctct cgaccgtagc gacgaggtgg tcgcgcagcg
    52021 ggcgtaaata acgggtgtga tagagctgca ggaaacgaga gcgaaactcc ggcacatcaa
    52081 ttttgatggg gcactgggtc gaacacgctt tacaggccag acagcccgac atcgcctctt
    52141 tgacttcgtg tgagaagtca tattcgcctt tattcgcatg ccagctattg cgcgtgcggg
    52201 caattaacgt ccgtaaactg acgccggatt caggcagttc ttgttccagt ttgagtggat
    52261 caacgccgcg atccgccaac aaacgcagcc attcacgcac cagcgttgcg cgccctttcg
    52321 gtgaatgaat ccggttctgg gtgatcttca tcgacggaca catcggacta cgggcatcaa
    52381 agttgaagca taaaccgttg ccgttacact ccatcgcacc gcgccactgc tggcgtaccg
    52441 caatggggat ctgccgatcg aatgtaccgc gcttcaccgc gtccactttc atcatcggcg
    52501 catcgagacc ttctggcggg caaatcttcc ctgggttgag tcggttatgc gggtcaaatg
    52561 ccgctttcac tttgcgcagt tctgcaaaaa gttcctcacc gaaaaacgcc gggctgtatt
    52621 cagcgcgaaa acctttgccg tgctcgcccc acaacaaacc accgtatttc gcagtcagcg
    52681 ccaccacgtc atcagagatt tgcttcatca aaatctcttg ttgcggatcg cacatatcca
    52741 gtgccggacg gacgtgcaaa acacctgcgt cgacgtgacc gaacataccg tagcttaagc
    52801 cgtggctgtc gagcagcgcg cgaaattcag caatataatc ggccagatgt tccggcggta
    52861 cgcaggtatc ctcagcaaac ggaattggct tagcggcacc tttggcattg ccaagcagac
    52921 caacggcttt tttgcgcatt gcatagatac gttcaacccc cgccagctcg cggcacacct
    52981 gccagccaat cacacctgcc tgttggctgg caatcagctc atcaagccgc acacagagtg
    53041 cattaactcg ctcgtcaatc agcgcctcat catcaccagc aaattccaca atgttcagcc
    53101 cgagcatctc tttgtcaggc acatcggtaa tcaactcgct gacggaatgc cagacaatat
    53161 cttcccgcgc cagattcagc acttttgagt ccaccgtctc taccgaaagc gcccgcgcct
    53221 caaccataaa cggcgcgtta cgcagcgcgg agtcaaaaga gtcatatttg acgttcacca
    53281 gacggcgcac tttaggcaag ggcgtaatat ccagccgcgc ttcggtaata aaggccagcg
    53341 tcccttctga acccgtcaga atgcgcgtca ggtcgaactc ggtcatctca tcgttaaaga
    53401 catgacgcag atcgtaaccg gtaagaaagc ggttaagttt ggggaagttg tcgatgatta
    53461 actggcgttg cagacggcaa cgttgataaa ccgtgttata aattcgcccg attgtggtat
    53521 tggttttacc cagcgtttcc gccagttcga cgggtaaagg ttgcgtatcg agaatatcgc
    53581 cccccaacaa caccgcgcgt acgccaagta cgtgatctga cgttttgcca tagaccagcg
    53641 atccctgacc ggatgcatcg gtattgatca tcccgccgag cgttgcccgg ttgctggtcg
    53701 aaagttctgg tgcaaaaaag tagccgaacg gtttcaggta ctgattgagt tgatctttta
    53761 tcaccccggc ctcaacgcgc acccagccct cttcagggtt aatttcgatg atgcggttca
    53821 tatggcggga catatcaaca ataatcccct ggttgagcgc ctgaccgtta gtgccggtgc
    53881 cgccgccgcg gggggtaaag atcagcgatg aatagcgttc ctgcgcggca agacgggcga
    53941 tcagcgccac atctgcggtt gaacgcggaa ataccaccgc atcggggaga agttggtaaa
    54001 tactgttgtc ggtcgacatt gtcagacgat cggcataact tgtcgccgta tcgccggtaa
    54061 aaccttgttg ctccagctct tgcaaaaaat taagcaccag ttgaacgacg ccgggtgcct
    54121 gggaaatctg tggaatcatt atattgaccc tttcctgcgg tctgtgatgt aggtcgatac
    54181 actattcttt caggcggctg caatagcgca ctgaaaggtg atgtttattt actctatgga
    54241 tttcgagttg caggaaggcg gcaagcgagt gaactccagg agcttacaat agtaagtgac
    54301 tggggtgaac gaacgtagcc gcagcgcatg caacttgaaa tacgacgagt aaatcgtttg
    54361 cgtgttgcct gagttgttgt accacatttt tttctaacac gcccatcaga attaagggca
    54421 gaatcggcct gttaaaaacc gctgaaattg ctcatcatta tgcaggtgag tttcgcgtgt
    54481 tcacgtcgcg tcgacgattt gacgcacaaa aaaggtgaaa agtagttatg gtaaatgttc
    54541 gtcagcccag ggatgtcgca caaattctgc tttcggtgct gtttttagcc atcatgattg
    54601 tggcatgtct gtggattgtt caacccttta ttctcggctt tgcatgggcc ggtacggtgg
    54661 ttatcgccac ctggccggta ttgttacgtt tgcaaaagat catgtttggc cgccgctctc
    54721 tcgccgttct ggtgatgacg ctgttattag tgatggtgtt tatcatccct attgctttgc
    54781 tggttaacag tatcgtcgac ggcagcggcc cgctaattaa agccatttcc agcggcgaca
    54841 tgacgttacc cgatctggcg tggcttaata ccattccggt gattggcgcg aagctgtatg
    54901 caggctggca caacttgctg gatatggggg gtacggcgat catggcgaaa gtccgccctt
    54961 atattggcac caccaccacc tggttcgttg ggcaggcggc gcatatcggg cgctttatgg
    55021 tgcattgtgc gctgatgctt ctcttcagtg ccctgctgta ctggcgcggt gaacaggtgg
    55081 cacaaggcat tcgccatttt gcaacccgtc tggcaggcgt tcgcggagat gccgccgtgc
    55141 tgctggcggc acaggctatc cgcgcggtgg cgctgggtgt ggtggtgacg gcgttagtac
    55201 aggcagtgct tggcagtatc ggcctcgccg tatccggcgt accttatgca acgttgctaa
    55261 cggtgttaat gatcctctcc tgccttgtcc agcttggccc gttgccggta ctgattccgg
    55321 cgattatctg gctctactgg a
//
LOCUS       DAJVYM010000010        49831 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVYM010000010 DAJVYM010000000
VERSION     DAJVYM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.010"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..455
                     /locus_tag="OQS60_000571"
     CDS             <1..455
                     /locus_tag="OQS60_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS2386425.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OQS60_000572"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OQS60_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS2386426.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OQS60_000573"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OQS60_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS2386427.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OQS60_000574"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OQS60_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS2386428.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OQS60_000575"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OQS60_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS2386429.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OQS60_000576"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OQS60_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS2386430.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OQS60_000577"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OQS60_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS2386431.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OQS60_000578"
     CDS             complement(6424..6918)
                     /locus_tag="OQS60_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS2386432.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OQS60_000579"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OQS60_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS2386433.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OQS60_000580"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OQS60_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS2386434.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OQS60_000581"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OQS60_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS2386435.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OQS60_000582"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OQS60_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS2386436.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OQS60_000583"
     CDS             complement(12082..12369)
                     /locus_tag="OQS60_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS2386437.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OQS60_000584"
     CDS             complement(12488..13375)
                     /locus_tag="OQS60_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS2386438.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OQS60_000585"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OQS60_000585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS2386439.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OQS60_000586"
     CDS             complement(14612..15106)
                     /locus_tag="OQS60_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS2386440.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OQS60_000587"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OQS60_000587"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS2386441.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OQS60_000588"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OQS60_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS2386442.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OQS60_000589"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OQS60_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS2386443.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OQS60_000590"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OQS60_000590"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS2386444.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OQS60_000591"
     CDS             19086..19745
                     /locus_tag="OQS60_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2386445.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OQS60_000592"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OQS60_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS2386446.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OQS60_000593"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OQS60_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS2386447.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OQS60_000594"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OQS60_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS2386448.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="OQS60_000595"
     CDS             23173..24099
                     /locus_tag="OQS60_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS2386449.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="OQS60_000596"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="OQS60_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS2386450.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="OQS60_000597"
     CDS             complement(24450..26669)
                     /locus_tag="OQS60_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS2386451.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OQS60_000598"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OQS60_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS2386452.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OQS60_000599"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OQS60_000599"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS2386453.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OQS60_000600"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OQS60_000600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS2386454.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="OQS60_000601"
     CDS             complement(32041..32523)
                     /locus_tag="OQS60_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2386455.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OQS60_000602"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OQS60_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS2386456.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="OQS60_000603"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="OQS60_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS2386457.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="OQS60_000604"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="OQS60_000604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS2386458.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="OQS60_000605"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="OQS60_000605"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS2386459.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="OQS60_000606"
     CDS             35847..36161
                     /locus_tag="OQS60_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS2386460.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OQS60_000607"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OQS60_000607"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS2386461.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OQS60_000608"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OQS60_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS2386462.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OQS60_000609"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OQS60_000609"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS2386463.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="OQS60_000610"
     CDS             complement(39563..39985)
                     /locus_tag="OQS60_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS2386464.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OQS60_000611"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OQS60_000611"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS2386465.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="OQS60_000612"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="OQS60_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS2386466.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="OQS60_000613"
     CDS             42449..43120
                     /locus_tag="OQS60_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS2386467.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="OQS60_000614"
     CDS             43126..44286
                     /locus_tag="OQS60_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS2386468.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OQS60_000615"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OQS60_000615"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS2386469.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="OQS60_000616"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="OQS60_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS2386470.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="OQS60_000617"
     CDS             complement(46086..46256)
                     /locus_tag="OQS60_000617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386471.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OQS60_000618"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OQS60_000618"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS2386472.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="OQS60_000619"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="OQS60_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS2386473.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="OQS60_000620"
     CDS             48117..48668
                     /locus_tag="OQS60_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS2386474.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..49831
                     /locus_tag="OQS60_000621"
                     /pseudo
     CDS             48728..49831
                     /locus_tag="OQS60_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT        12496 a        13205 c        12358 g        11772 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgacta
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgttatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggccagt
    49741 ctatatatca atgacactaa ccattacatg aattatcaga atgaaaaaga gagttatatt
    49801 gtcacgttta accagtatct tgaatcatta a
//
LOCUS       DAJVYM010000011        45926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVYM010000011 DAJVYM010000000
VERSION     DAJVYM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.011"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..715)
                     /locus_tag="OQS60_000622"
     CDS             complement(<1..715)
                     /locus_tag="OQS60_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS2386475.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLESADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="OQS60_000623"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="OQS60_000623"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS2386476.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="OQS60_000624"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="OQS60_000624"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS2386477.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="OQS60_000625"
     CDS             complement(3649..4704)
                     /locus_tag="OQS60_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2386478.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="OQS60_000626"
     CDS             complement(4714..5502)
                     /locus_tag="OQS60_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS2386479.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="OQS60_000627"
     CDS             complement(5520..6572)
                     /locus_tag="OQS60_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS2386480.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7206)
                     /locus_tag="OQS60_000628"
                     /pseudo
     CDS             complement(6603..7206)
                     /locus_tag="OQS60_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7193..8074)
                     /locus_tag="OQS60_000629"
     CDS             complement(7193..8074)
                     /locus_tag="OQS60_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS2386481.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8095..9405)
                     /locus_tag="OQS60_000630"
     CDS             complement(8095..9405)
                     /locus_tag="OQS60_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2386482.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9440..10950)
                     /locus_tag="OQS60_000631"
                     /pseudo
     CDS             complement(9440..10950)
                     /locus_tag="OQS60_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="OQS60_000632"
     CDS             complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="OQS60_000632"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS2386483.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="OQS60_000633"
     CDS             complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="OQS60_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS2386484.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="OQS60_000634"
     CDS             complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="OQS60_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS2386485.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="OQS60_000635"
     CDS             complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="OQS60_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS2386486.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="OQS60_000636"
     CDS             complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="OQS60_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS2386487.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13253..14230
                     /gene="pspF"
                     /locus_tag="OQS60_000637"
     CDS             13253..14230
                     /gene="pspF"
                     /locus_tag="OQS60_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS2386488.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="OQS60_000638"
                     /pseudo
     CDS             complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="OQS60_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="OQS60_000639"
     CDS             complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="OQS60_000639"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS2386489.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="OQS60_000640"
                     /pseudo
     CDS             complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="OQS60_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="OQS60_000641"
     CDS             complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="OQS60_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS2386490.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="OQS60_000642"
     CDS             complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="OQS60_000642"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS2386491.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20133..21494
                     /gene="puuA"
                     /locus_tag="OQS60_000643"
     CDS             20133..21494
                     /gene="puuA"
                     /locus_tag="OQS60_000643"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS2386492.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21643..21807
                     /locus_tag="OQS60_000644"
     CDS             21643..21807
                     /locus_tag="OQS60_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386493.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21797..23182
                     /gene="puuP"
                     /locus_tag="OQS60_000645"
     CDS             21797..23182
                     /gene="puuP"
                     /locus_tag="OQS60_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS2386494.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23316..23561
                     /locus_tag="OQS60_000646"
     CDS             23316..23561
                     /locus_tag="OQS60_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS2386495.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23874..25517
                     /gene="sapA"
                     /locus_tag="OQS60_000647"
     CDS             23874..25517
                     /gene="sapA"
                     /locus_tag="OQS60_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS2386496.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25514..26479
                     /gene="sapB"
                     /locus_tag="OQS60_000648"
     CDS             25514..26479
                     /gene="sapB"
                     /locus_tag="OQS60_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS2386497.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26466..27356
                     /gene="sapC"
                     /locus_tag="OQS60_000649"
     CDS             26466..27356
                     /gene="sapC"
                     /locus_tag="OQS60_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS2386498.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27356..28348
                     /gene="sapD"
                     /locus_tag="OQS60_000650"
     CDS             27356..28348
                     /gene="sapD"
                     /locus_tag="OQS60_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS2386499.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28350..29156
                     /gene="sapF"
                     /locus_tag="OQS60_000651"
     CDS             28350..29156
                     /gene="sapF"
                     /locus_tag="OQS60_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS2386500.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29224..29577
                     /locus_tag="OQS60_000652"
     CDS             29224..29577
                     /locus_tag="OQS60_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS2386501.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29945..30733
                     /gene="fabI"
                     /locus_tag="OQS60_000653"
     CDS             29945..30733
                     /gene="fabI"
                     /locus_tag="OQS60_000653"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS2386502.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30878..32004
                     /locus_tag="OQS60_000654"
                     /pseudo
     CDS             30878..32004
                     /locus_tag="OQS60_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32072..34006
                     /gene="rnb"
                     /locus_tag="OQS60_000655"
     CDS             32072..34006
                     /gene="rnb"
                     /locus_tag="OQS60_000655"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS2386503.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34084..34187)
                     /locus_tag="OQS60_000656"
                     /pseudo
     CDS             complement(34084..34187)
                     /locus_tag="OQS60_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34241..36231
                     /gene="pdeR"
                     /locus_tag="OQS60_000657"
                     /pseudo
     CDS             34241..36231
                     /gene="pdeR"
                     /locus_tag="OQS60_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36378..36551
                     /locus_tag="OQS60_000658"
     CDS             36378..36551
                     /locus_tag="OQS60_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386504.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36641..37390
                     /locus_tag="OQS60_000659"
     CDS             36641..37390
                     /locus_tag="OQS60_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2386505.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37659..37877
                     /gene="osmB"
                     /locus_tag="OQS60_000660"
     CDS             37659..37877
                     /gene="osmB"
                     /locus_tag="OQS60_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS2386506.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="OQS60_000661"
     CDS             complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="OQS60_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS2386507.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="OQS60_000662"
     CDS             complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="OQS60_000662"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS2386508.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="OQS60_000663"
     CDS             complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="OQS60_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS2386509.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="OQS60_000664"
     CDS             complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="OQS60_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS2386510.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="OQS60_000665"
     CDS             complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="OQS60_000665"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS2386511.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41826..42416
                     /gene="ribA"
                     /locus_tag="OQS60_000666"
     CDS             41826..42416
                     /gene="ribA"
                     /locus_tag="OQS60_000666"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS2386512.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="OQS60_000667"
     CDS             complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="OQS60_000667"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS2386513.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45319..45414)
                     /locus_tag="OQS60_000668"
     CDS             complement(45319..45414)
                     /locus_tag="OQS60_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386514.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45528..45695)
                     /locus_tag="OQS60_000669"
     CDS             complement(45528..45695)
                     /locus_tag="OQS60_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386515.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45698..45862)
                     /locus_tag="OQS60_000670"
     CDS             complement(45698..45862)
                     /locus_tag="OQS60_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS2386516.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10847 a        12143 c        11561 g        11375 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcactttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctccccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7081 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7141 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7201 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7261 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7321 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7381 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7441 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7501 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7561 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7621 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7681 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7741 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7801 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7861 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7921 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7981 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8041 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8101 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8161 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8221 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8281 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8341 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8401 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8461 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8521 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8581 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8641 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8701 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8761 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8821 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     8881 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8941 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9001 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9061 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9121 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9181 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9241 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9301 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9361 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9421 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     9481 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9541 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     9601 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9661 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9721 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9781 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9841 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9901 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9961 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10021 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10081 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10141 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10201 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10261 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10321 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10381 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10441 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10501 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10561 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10621 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10681 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10741 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10801 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10861 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10921 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10981 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11041 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11101 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11161 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11221 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11281 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11341 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11401 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11461 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11521 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11581 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11641 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11701 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11761 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11821 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11881 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    11941 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12001 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12061 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12121 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12181 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12241 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12301 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12361 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12421 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12481 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12541 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12601 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12661 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12721 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12781 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12841 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12901 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12961 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13021 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13081 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13141 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13201 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13261 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13321 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13381 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13441 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13501 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13561 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13621 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13681 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13741 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13801 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13861 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13921 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13981 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14041 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14101 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14161 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14221 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14281 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14341 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14401 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14461 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14521 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14581 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14641 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14701 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14761 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14821 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14881 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14941 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15001 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15061 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15121 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15181 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15241 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15301 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15361 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15421 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15481 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15541 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15601 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15661 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15721 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15781 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15841 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15901 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15961 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16021 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16081 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16141 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16201 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16261 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16321 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16381 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16441 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16501 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16561 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16621 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16681 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16741 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16801 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16861 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16921 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16981 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17041 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17101 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17161 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17221 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17281 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17341 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17401 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17461 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17521 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17581 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17641 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17701 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17761 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17821 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17881 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17941 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18001 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18061 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18121 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18181 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18241 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    18301 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18361 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18421 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18481 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18541 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18601 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18661 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18721 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18781 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18841 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18901 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18961 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19021 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19081 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19141 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19201 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19261 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19321 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19381 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19441 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19501 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19561 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19621 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19681 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19741 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19801 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19861 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19921 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19981 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20041 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20101 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20161 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20221 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20281 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20341 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20401 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20461 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20521 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20581 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20641 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20701 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20761 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20821 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20881 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20941 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21001 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21061 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21121 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21181 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21241 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21301 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21361 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21421 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21481 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21541 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21601 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21661 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21721 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21781 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21841 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21901 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21961 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22021 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22081 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22141 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22201 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22261 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22321 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22381 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22441 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22501 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22561 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22621 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22681 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22741 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22801 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22861 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22921 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22981 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23041 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23101 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23161 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23221 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    23281 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23341 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23401 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23461 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23521 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23581 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23641 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23701 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23761 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23821 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23881 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23941 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24001 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24061 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24121 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24181 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24241 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24301 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24361 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24421 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24481 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24541 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24601 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24661 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24721 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24781 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24841 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24901 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24961 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25021 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25081 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25141 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    25201 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25261 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25321 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25381 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25441 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25501 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25561 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25621 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25681 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25741 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25801 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25861 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25921 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25981 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26041 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26101 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26161 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26221 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26281 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26341 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26401 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26461 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26521 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26581 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26641 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26701 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26761 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26821 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26881 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26941 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27001 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27061 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27121 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27181 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27241 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27301 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27361 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27421 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27481 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27541 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27601 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27661 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27721 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27781 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27841 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27901 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27961 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28021 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28081 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28141 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28201 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28261 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28321 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28381 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28441 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28501 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28561 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28621 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28681 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28741 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28801 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28861 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28921 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28981 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29041 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29101 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29161 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29221 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29281 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29341 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29401 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29461 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29521 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29581 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29641 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29701 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29761 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29821 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29881 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29941 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30001 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30061 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30121 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30181 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30241 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30301 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30361 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30421 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30481 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30541 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30601 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30661 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30721 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30781 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30841 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30901 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30961 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31021 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31081 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31141 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31201 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31261 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31321 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31381 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31441 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31501 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31561 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31621 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31681 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31741 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31801 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31861 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31921 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31981 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32041 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32101 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32161 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    32221 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32281 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32341 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32401 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32461 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32521 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32581 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32641 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32701 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32761 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32821 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32881 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32941 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33001 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33061 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33121 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33181 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33241 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33301 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33361 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33421 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33481 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33541 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33601 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33661 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33721 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33781 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33841 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33901 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33961 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34021 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34081 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34141 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34201 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34261 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34321 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34381 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34441 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34501 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34561 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34621 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34681 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34741 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34801 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34861 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34921 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34981 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    35041 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35101 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35161 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35221 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35281 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35341 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35401 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35461 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35521 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35581 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35641 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35701 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35761 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35821 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35881 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35941 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36001 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36061 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36121 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36181 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36241 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36301 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36361 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36421 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36481 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36541 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36601 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36661 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36721 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36781 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36841 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    36901 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36961 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37021 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37081 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37141 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37201 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37261 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37321 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37381 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37441 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37501 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37561 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37621 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37681 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37741 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37801 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37861 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37921 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37981 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38041 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38101 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38161 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38221 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38281 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38341 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38401 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38461 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38521 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38581 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38641 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38701 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38761 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38821 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38881 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38941 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39001 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39061 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39121 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39181 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39241 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39301 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39361 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39421 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39481 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39541 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39601 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39661 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39721 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39781 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39841 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39901 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39961 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40021 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40081 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40141 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40201 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40261 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40321 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40381 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40441 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40501 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40561 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40621 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40681 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40741 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40801 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40861 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40921 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40981 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41041 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41101 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41161 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41221 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41281 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41341 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41401 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    41461 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41521 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41581 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41641 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41701 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41761 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41821 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41881 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41941 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42001 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42061 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42121 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42181 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42241 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42301 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42361 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42421 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42481 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42541 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42601 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42661 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42721 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42781 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42841 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42901 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42961 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43021 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43081 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43141 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43201 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43261 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43321 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43381 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43441 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43501 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43561 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43621 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43681 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43741 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43801 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43861 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43921 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43981 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44041 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44101 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44161 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44221 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44281 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44341 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44401 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44461 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44521 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44581 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44641 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44701 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44761 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44821 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44881 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44941 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45001 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45061 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45121 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45181 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45241 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45301 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45361 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45421 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45481 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45541 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45601 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45661 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45721 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45781 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45841 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45901 caaatcctaa agaaacttta gtcaaa
//
LOCUS       DAJVYM010000012        44841 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVYM010000012 DAJVYM010000000
VERSION     DAJVYM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.012"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..340
                     /locus_tag="OQS60_000671"
     CDS             <1..340
                     /locus_tag="OQS60_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS2386517.1"
                     /translation="RYSAAIRSGDLLFVSGQVGSREDGTPEPDFQQQVRLAFDNLHAT
                     LAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAPPYPNWTAVGVTWLAGFDFE
                     IKVIARIPEQ"
     gene            621..755
                     /gene="pyrL"
                     /locus_tag="OQS60_000672"
     CDS             621..755
                     /gene="pyrL"
                     /locus_tag="OQS60_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS2386518.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            759..1694
                     /gene="pyrB"
                     /locus_tag="OQS60_000673"
     CDS             759..1694
                     /gene="pyrB"
                     /locus_tag="OQS60_000673"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS2386519.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            1707..2168
                     /gene="pyrI"
                     /locus_tag="OQS60_000674"
     CDS             1707..2168
                     /gene="pyrI"
                     /locus_tag="OQS60_000674"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS2386520.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            2241..2627
                     /gene="ridA"
                     /locus_tag="OQS60_000675"
     CDS             2241..2627
                     /gene="ridA"
                     /locus_tag="OQS60_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS2386521.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(2833..5529)
                     /gene="mgtA"
                     /locus_tag="OQS60_000676"
     CDS             complement(2833..5529)
                     /gene="mgtA"
                     /locus_tag="OQS60_000676"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS2386522.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(5670..5723)
                     /gene="mgtL"
                     /locus_tag="OQS60_000677"
     CDS             complement(5670..5723)
                     /gene="mgtL"
                     /locus_tag="OQS60_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS2386523.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            5908..6855
                     /gene="treR"
                     /locus_tag="OQS60_000678"
     CDS             5908..6855
                     /gene="treR"
                     /locus_tag="OQS60_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS2386524.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            6974..8395
                     /gene="treB"
                     /locus_tag="OQS60_000679"
     CDS             6974..8395
                     /gene="treB"
                     /locus_tag="OQS60_000679"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS2386525.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            8445..10100
                     /gene="treC"
                     /locus_tag="OQS60_000680"
     CDS             8445..10100
                     /gene="treC"
                     /locus_tag="OQS60_000680"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS2386526.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            10494..12632
                     /gene="nrdD"
                     /locus_tag="OQS60_000681"
     CDS             10494..12632
                     /gene="nrdD"
                     /locus_tag="OQS60_000681"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS2386527.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            12790..13254
                     /gene="nrdG"
                     /locus_tag="OQS60_000682"
     CDS             12790..13254
                     /gene="nrdG"
                     /locus_tag="OQS60_000682"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS2386528.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(13299..13685)
                     /gene="cybC"
                     /locus_tag="OQS60_000683"
     CDS             complement(13299..13685)
                     /gene="cybC"
                     /locus_tag="OQS60_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS2386529.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(13869..15221)
                     /gene="pmbA"
                     /locus_tag="OQS60_000684"
     CDS             complement(13869..15221)
                     /gene="pmbA"
                     /locus_tag="OQS60_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS2386530.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            15315..15866
                     /locus_tag="OQS60_000685"
     CDS             15315..15866
                     /locus_tag="OQS60_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS2386531.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(15949..17322)
                     /gene="mpl"
                     /locus_tag="OQS60_000686"
     CDS             complement(15949..17322)
                     /gene="mpl"
                     /locus_tag="OQS60_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS2386532.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            17498..18496
                     /gene="fbp"
                     /locus_tag="OQS60_000687"
     CDS             17498..18496
                     /gene="fbp"
                     /locus_tag="OQS60_000687"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS2386533.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(18529..19524)
                     /gene="yjfF"
                     /locus_tag="OQS60_000688"
     CDS             complement(18529..19524)
                     /gene="yjfF"
                     /locus_tag="OQS60_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS2386534.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(19511..20533)
                     /locus_tag="OQS60_000689"
     CDS             complement(19511..20533)
                     /locus_tag="OQS60_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2386535.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(20547..22049)
                     /locus_tag="OQS60_000690"
     CDS             complement(20547..22049)
                     /locus_tag="OQS60_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS2386536.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(22189..23145)
                     /gene="ytfQ"
                     /locus_tag="OQS60_000691"
     CDS             complement(22189..23145)
                     /gene="ytfQ"
                     /locus_tag="OQS60_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS2386537.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            23455..23985
                     /gene="ppa"
                     /locus_tag="OQS60_000692"
     CDS             23455..23985
                     /gene="ppa"
                     /locus_tag="OQS60_000692"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS2386538.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(24364..24705)
                     /locus_tag="OQS60_000693"
     CDS             complement(24364..24705)
                     /locus_tag="OQS60_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS2386539.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(24708..28487)
                     /gene="tamB"
                     /locus_tag="OQS60_000694"
     CDS             complement(24708..28487)
                     /gene="tamB"
                     /locus_tag="OQS60_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS2386540.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(28484..30217)
                     /gene="tamA"
                     /locus_tag="OQS60_000695"
     CDS             complement(28484..30217)
                     /gene="tamA"
                     /locus_tag="OQS60_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS2386541.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            30423..31061
                     /gene="msrA"
                     /locus_tag="OQS60_000696"
     CDS             30423..31061
                     /gene="msrA"
                     /locus_tag="OQS60_000696"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS2386542.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            31384..32727
                     /locus_tag="OQS60_000697"
     CDS             31384..32727
                     /locus_tag="OQS60_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS2386543.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(32807..33013)
                     /locus_tag="OQS60_000698"
     CDS             complement(32807..33013)
                     /locus_tag="OQS60_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS2386544.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            33338..33892
                     /locus_tag="OQS60_000699"
     CDS             33338..33892
                     /locus_tag="OQS60_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS2386545.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(33982..34893)
                     /locus_tag="OQS60_000700"
     CDS             complement(33982..34893)
                     /locus_tag="OQS60_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386546.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(35105..35845)
                     /gene="cysQ"
                     /locus_tag="OQS60_000701"
     CDS             complement(35105..35845)
                     /gene="cysQ"
                     /locus_tag="OQS60_000701"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS2386547.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            36035..37978
                     /gene="cpdB"
                     /locus_tag="OQS60_000702"
     CDS             36035..37978
                     /gene="cpdB"
                     /locus_tag="OQS60_000702"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS2386548.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(38096..38476)
                     /locus_tag="OQS60_000703"
     CDS             complement(38096..38476)
                     /locus_tag="OQS60_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS2386549.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            38565..39425
                     /gene="qorB"
                     /locus_tag="OQS60_000704"
     CDS             38565..39425
                     /gene="qorB"
                     /locus_tag="OQS60_000704"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS2386550.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            39533..40498
                     /locus_tag="OQS60_000705"
     CDS             39533..40498
                     /locus_tag="OQS60_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS2386551.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            40606..41268
                     /gene="ytfE"
                     /locus_tag="OQS60_000706"
     CDS             40606..41268
                     /gene="ytfE"
                     /locus_tag="OQS60_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS2386552.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(41313..42725)
                     /gene="cycA"
                     /locus_tag="OQS60_000707"
                     /gene_synonym="dagA"
     CDS             complement(41313..42725)
                     /gene="cycA"
                     /locus_tag="OQS60_000707"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS2386553.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(43034..43654)
                     /gene="fklB"
                     /locus_tag="OQS60_000708"
     CDS             complement(43034..43654)
                     /gene="fklB"
                     /locus_tag="OQS60_000708"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS2386554.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            43873..44511
                     /gene="ytfB"
                     /locus_tag="OQS60_000709"
     CDS             43873..44511
                     /gene="ytfB"
                     /locus_tag="OQS60_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCS2386555.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(44495..>44841)
                     /locus_tag="OQS60_000710"
     CDS             complement(44495..>44841)
                     /locus_tag="OQS60_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS2386556.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11005 a        11950 c        11182 g        10704 t
ORIGIN      
        1 tcgttattct gcggctattc gttccggcga tttgctgttt gtttccgggc aagtaggaag
       61 tcgagaggac ggaacaccag aacccgattt tcagcaacaa gtcagactgg catttgataa
      121 tttgcatgcg accctggctg ctgcgggatg cacttttgac gatatcattg atgttacgag
      181 cttccatacc gatccagaaa accaatttga agacatcatg acggtgaaaa atgaaatatt
      241 tagcgcccca ccttatccaa actggacggc ggtaggtgtt acatggctgg caggctttga
      301 ttttgaaatt aaagtgatag cgcgcatccc tgagcagtaa gcaatagtgt tagccgttcg
      361 ttttcacact ccgccctata agtcggatga atggaataaa atgcatatct gattgcgtga
      421 aagtgaaaaa ggaaaaagca ggaaatgtct gcaattattg ataccgaacg accgttcccc
      481 tgcagaatca caacaaataa aaatgcatgt attttgactt ataattcaaa taaaccgttt
      541 gcgctgacaa aatattgcat caaatgcttg caccgcttct gacgatgagt ataatgccgg
      601 acaatttgcc gggaggatgt atggttcagt gtgttcgaca ttttgtctta ccgcgtctga
      661 aaaaagacgc tggcctgccg tttttcttcc cgttgatcac ccattcccag cccctcaatc
      721 gaggggcttt tttttgccca ggcgtcagga gataaaagat ggctaatccg ctatatcaga
      781 aacatatcat ttccataaac gaccttagtc gcgatgacct taatctggtg ctggcgacag
      841 cggcgaaact gaaagcaaac ccgcaaccag agctgttgaa gcacaaagtc attgccagct
      901 gtttcttcga agcctctacc cgtacccgcc tctctttcga aacttccatg caccgcctgg
      961 gtgccagcgt ggtgggcttc tccgacagcg ccaatacatc actgggtaaa aagggcgaaa
     1021 cgctggccga taccatttcg gttatcagca cttacgtcga tgcgatagtg atgcgtcatc
     1081 cgcaggaagg tgcggcgcgc ctggccaccg agttttccgg caatgtaccg gtactgaatg
     1141 ccggtgatgg ctccaaccaa catccgacgc aaaccttgct ggacttattc actattcagg
     1201 aaacccaggg gcgtctggac aatctccacg tcgcaatggt tggtgacctg aaatatggcc
     1261 gcaccgttca ctccctgact caggcgttag cgaagttcga cggcaaccgt ttttacttca
     1321 tcgcgccgga cgcgctggca atgccgcaat acattctgga tatgcttgat gaaaaaggga
     1381 tcgcctggag tctgcacagc tctattgaag aagtgatggc ggaagtagac atcctgtaca
     1441 tgacccgcgt gcaaaaagag cgtctggacc cgtccgagta cgccaacgtg aaagcgcagt
     1501 ttgttcttcg cgccagcgat ctccacaacg ccaaagccaa tatgaaagtg ctgcatccgc
     1561 tgccgcgtgt tgatgagatt gcgacggatg ttgataaaac gccacacgcc tggtacttcc
     1621 agcaggcagg caacgggatt ttcgctcgcc aggcgttact ggcactggtt ctgaatcgcg
     1681 atctggtact gtaaggggaa atagagatga cacacgataa taaattgcag gttgaagcta
     1741 ttaaacgcgg cacggtaatt gaccatatcc ccgcccagat cggttttaag ctgttgagtc
     1801 tgttcaagct gaccgaaacg gatcagcgca tcaccattgg tctgaacctg ccttctggcg
     1861 agatgggccg caaagatctg atcaaaatcg aaaatacctt tttgagtgaa gatcaggtag
     1921 atcaactggc attgtatgcg ccgcaagcca cggttaaccg tatcgacaac tatgaagtgg
     1981 tgggtaaatc acgccccagt ctgccggagc gcatcgacaa tgtgctggtc tgcccgaaca
     2041 gcaactgtat cagccatgcc gaaccggttt cttccagctt tgccgtgcga aaacgcgcca
     2101 atgacatcgc gctcaaatgc aaatactgtg aaaaagagtt ttcccataat gtggtgctgg
     2161 ccaattaatt gcggttggta ataaaagtct ggctccctat aatgagccag actttttacc
     2221 gctgtaataa aggagaaatc atgagcaaaa ctatcgcgac ggaaaatgca ccggcagcta
     2281 tcggtcctta cgtacagggc gttgatctgg gcaatatgat catcacctcc ggtcagatcc
     2341 cggtaaatcc gaaaacgggc gaagtaccgg cagacgtcgc tgcacaggca cgtcagtcgc
     2401 tggataacgt aaaagcgatc gtcgaagccg ctggcctgaa agtgggcgac atcgttaaaa
     2461 ccaccgtgtt tgtgaaagat ctgaacgact tcgcaaccgt taatgctacc tacgaagcct
     2521 tcttcaccga acacaatgcc accttcccgg cacgttcttg cgttgaagtt gcccgtctgc
     2581 cgaaagacgt gaaaattgag atcgaagcga tcgctgttcg tcgctaatct tgatggaaat
     2641 ccgggctatc atgcccggat taagtctgat gacaaacaca aaatcgcctg atgcgctacg
     2701 cttatcaggc ctacgtgatt cctgcaattt attgaatttg ttggccggat aaggcattta
     2761 cgccgcatcc ggcatgaaca aaactcactt tgtctgcaat ctgaatccag gccaccatgc
     2821 ccagttgatt ctttattgcc agccgtaacg acggctataa aatcctttca ccagttgggt
     2881 taacgtcata taccctgcca gaatcgcaac cagccacggg aaatagctta acggcagcgc
     2941 ctgcagttgc agataactgg ccagcggtga aaacggcaat gcgatcccga caatcatcac
     3001 gatcacggtc atgatcatta acggccacga tgcacagctc tgaataaacg gcacacggcg
     3061 ggtgcggatc atatgcacaa tcagcgtttg cgacagcaag cccaccacaa accatcccga
     3121 ctggaacagc gtttgcgttt ccggcgtgtt ggcatggaat acccaccaca tcaggcaaaa
     3181 cgtcagaata tcgaagatcg agctgatcgg tccgaagaag atcataaagc gccccagatc
     3241 cgccggattc caacgctgcg gcttttgaat ttgctcgtcg tcgacgttat caaacgggat
     3301 cgccacctgt gacacatcat acagcaggtt ctgaataagc aagtgtaacg gcaacatcgg
     3361 caggaagggc aagaaagcac tcgccaccag cacgctgaac acattaccga agttggagct
     3421 cgccgtcatt ttgatgtatt tcagcatgtt ggcgaaagtg cgacgtcctt caataacccc
     3481 ctcttccagc accatcaggc ttttttccag caggatgata tcagccgctt cacgggcaat
     3541 atctaccgcg ccgtccacag aaatgccgat atccgcagcg cgtaaggcgg gcgcatcatt
     3601 aataccatcg cccataaagc caaccacatg cccttcgcgc ttcagtaagg tcacaatgcg
     3661 ttctttatgc atcggcgtca gacgagcaaa cagcgtggtg cgctgtgcga gatttgccag
     3721 ttcgtcgtca gatagcgttt caatatcact accaatgacc acctctcccg cgtccagccc
     3781 cacttcatgg cacactttcg ctgcaactaa ctcactgtcg ccagtaagga tttttacggt
     3841 aattccactc gcttttaatg ccttcagtgc cggagcagtt gtctcttttg gcggatcaag
     3901 gaaagcaata tatccttcga ggatcaggtc ggattcatcc gcccgctggt aatctccttc
     3961 acgcgctggc aggtatttcg tcgccaccgc aaccacgcgc agcccctgac gattcagcgt
     4021 atcagtaacc cgcttaatct tacgcagcat gatgtcatcg agcggcacaa tctcgccatt
     4081 gtgacgcacc tgcgaacaca cattgaggat ttcctgcaat gcacctttgc aaaccagctg
     4141 atggtgctcg gtattttctg ccactaccac cgacatccgg cgacgctcga aatcaaacgg
     4201 aatctcatca attttttgcc aacggctggc cagagagcgc gctgactctt catccgtacc
     4261 ttcgagcacc gctgtatcga gtaggttttt aagtccggtc tgataatgac tgttcaacca
     4321 cgcgctatgc agcacgcgtt cgctggtttt accggagata tcggtatgat tctccagcac
     4381 aattttgtcc tgtgtcaggg tgccagtttt atcagtgcac agaatatcca ttgcgccaaa
     4441 gttctgaata gcatccagat gtttgacgat cactttctgt ttcgacagct ttactgcgcc
     4501 gcgcgccagc gtcgaggtaa caatcatcgg caacatttcc ggcgttaagc ctaccgcgac
     4561 cgaaagcgca aacagtgccg cttcccacca gtcgcctttg gtgtaaccgt tgataagcaa
     4621 caccaccgga gccatcacca gcataaagcg aatcagcagc atactgacgc ggctgatccc
     4681 ctgctgaaag gcattcggct cgctttcctg ctcactaaca cgccccgcca gttgaccaaa
     4741 ccaggtgttg gctcctgtag caatcaccat tgcttgtgcc gtgccgctca ccacggtggt
     4801 gcccataaaa cacagggtgt cacactccag cggattgctg tgctccggct ggcgagtggt
     4861 agcggctttt tccacgggca gagactcacc ggttaacgac gcctgagcga cgaacagatc
     4921 ccgcgcctgc aagatacgta aatctgccgg gatcatatcc cccgccgcca gttttataat
     4981 atcgccgggc accagctggt cgatcgggat ctccagccag ccattttcgc ctttgtcgtt
     5041 aattacgcgc agcaccgtcg cggtattgct gaccatcgct ttcagggcat ctgccgcttt
     5101 agtggaacgt gcttcctgaa taaagttcag caacgtagaa atagcgacca ttagcgcgat
     5161 aacgcccgcg gcaaataaat cttcagtggc gtaagaaata gcgccgagaa tggtgagtaa
     5221 aatattaaat gggttgcgat agcagaccca taaatgtacc caccacggcg acggttgttg
     5281 tgcgggtaat ttattttcac catgttgttc gcgggcagat tccacttccg cctgatttaa
     5341 cccttccgga tgggtgtcga acgttttcca caattcttct tcgggcatca ccgccatttt
     5401 caggcaatgc gcacttaagg acggcgggac cgccgtattc actgtctgct gcgcgccagg
     5461 caatggatcg cgatgaacca gacgggaagg taaatggcga atgagccggg taaaaatttc
     5521 tttaaacata aggagtccct ccgcactgtc tgaaacagta cagaaatgtc ttcaggcgtg
     5581 gcgaagcctt acctgaaggt aataaagctg agcagggacg ttaaaacgtc acggccttac
     5641 gcatccggta gcgccgtgaa aagacgggct taccggaaaa gttttaatct ccgtcgaggg
     5701 agaggcgtgg gatcaggttc catataacct ccggtaagtg aaaaaagcgc cgcaaataat
     5761 aaggagaatc caataatatt cgtagcattt atggcgatat aatacttgct ggcaactaaa
     5821 ccaacgataa accagacttt acccattgct gaatgcacgg gtaaagttag gctcaaataa
     5881 ttaaacaaca cgttacagga caacaggatg caaaatcggc tgactatcaa agacatcgca
     5941 cgcttaagcg gcgtggggaa atctacagtt tcccgggtgc tgaataacga aagcggcgtg
     6001 agccagcgca cccgcgagcg tgttgaagca gtgatgaatc agcatggatt ttccccttcc
     6061 cgctctgcgc gcgctatgcg tgggcaaagc gataaagtgg tcgccatcat tgttacccgt
     6121 ctggattcgt tgtcagaaaa tctcgccgtt caaaccatgc tgccagcgtt ctatgaacaa
     6181 ggttacgacc caatcatgat ggaaagccag ttttccccgc aattagttgc cgaacatttg
     6241 ggggtgctga aacggcgtaa tatcgacggc gtagtgctgt tcggttttac tggcataaca
     6301 gaagaaatgt tagcccactg gcagtcatcg ctggttctgc tggcgcgtga cgcaaaaggc
     6361 tttgcttcgg tctgttatga cgacgaaggg gcaatcaaga tcctgatgca acggctgtat
     6421 gaccaggggc atcgtaatat cagttatctc ggcgtgccgc atagtgacgt gacaaccggt
     6481 aagcgacgtc acgaagccta cctggcgttc tgcaaagcgc ataaactgca tcccgttgcc
     6541 gctctgccag ggcttgctat gaagcaaggc tatgagaacg tggcaaaagt gattacgcct
     6601 gaaactactg cgttactgtg cgcaaccgac acgctggcac ttggcgcaag taaatacctg
     6661 caagagcaac gcatcgacac cttgcaactg gcgagcgtcg gtaatacacc gttaatgaaa
     6721 ttcctccatc cggagatcgt caccgtagat cccggttacg ccgaagctgg acgccaggcg
     6781 gcctgccaat tgatcgcaca ggtaaccggg cgcagcgaac cgcaacaaat catcatcccc
     6841 gccaccctgt cctgatcgtt tcctgaacga taaattgtga tcttcgctgc atttcgggaa
     6901 cgttcccgtt tttaaatttt tccgcgcaat atattctgca gccaaccaaa aatgtcatct
     6961 gccatggggc tttatgatga gcaaaataaa ccaaacggat atcgatcggt tgattgaact
     7021 ggtcggcggg cgcggcaata ttgcgacggt gagccactgt attactcgcc tgcgctttgt
     7081 cctcaaccaa ccggccaatg ccagaccgaa agaaattgag caactcccca tggtgaaagg
     7141 ctgtttcacc aatgccgggc aatttcaggt ggtgattggc accaacgtgg gtgattacta
     7201 tcaagcacta atagcgtcaa ccggacaggc gcaggttgat aaagagcagg taaaaaaagc
     7261 cgcccggcag aatatgaaat ggcatgagca gttgatctct catttcgcgg agatcttctt
     7321 cccgttgctg cccgcgttga ttagcggcgg tttgatcctc ggttttcgca atgtgatcgg
     7381 cgatttgccc atgagcaacg gtcagacgct ggcgcaaatg tacccttccc tgcaaacgat
     7441 ctacgatttt ctgtggttga tcggtgaagc gattttcttc tacctgccgg tcgggatttg
     7501 ctggtcagcg gtgaaaaaaa tgggcggcac gccgatcctt ggtatcgtgc ttggcgtgac
     7561 actggtttcc ccccagctga tgaacgctta tctgctcggg cagcagctgc cggaagtgtg
     7621 ggactttggc atgttcagca tcgccaaagt gggctatcag gcgcaggtga tcccggcact
     7681 gttagccggg ctggcgctgg gcgttattga aactcgcctt aaacgcatcg taccggatta
     7741 cctctatctg gtggtggtgc ccgtctgttc gctgatcctc gcggtgttcc tcgcccatgc
     7801 gctgattggt ccgtttggtc gcatgattgg cgatggcgtt gcctttgcgg tacgtcacct
     7861 gatgaccggc agctttgctc cgattggtgc ggcattgttt ggcttcctgt acgcgccgct
     7921 ggtgatcacc ggcgtacacc agaccaccct tgctattgat ttgcagatga ttcaaagcat
     7981 gggtggcacg ccagtgtggc cgctgattgc gctgtcgaat atcgctcagg gctccgccgt
     8041 gataggcatt atcatttcca gccgcaagca caatgaacgc gagatctccg tgcctgccgc
     8101 tatctccgcc tggcttgggg tcactgagcc tgcaatgtac ggcatcaacc tgaaatatcg
     8161 cttcccgatg ctgtgcgcga tgattggttc tggtctggca ggattgctat gcggcctgaa
     8221 cggcgttatg gcgaatggta tcggcgtagg gggcctgccg ggaattctct cgattcaacc
     8281 gagctactgg caggtatttg cgctggcaat ggctatcgcc atcatcatcc cgattgtact
     8341 cacctcgttt atctatcagc ggaaataccg cttgggcacg ctggacattg tttaattttc
     8401 ttcggggcgc aattgcgccc cctcgcattt gcaggaataa cgtaatgact aatcttcccc
     8461 actggtggca aaacggcgtt atctaccaga tttatccaaa gagttttcag gacaccacgg
     8521 gtagcggcac cggcgattta cgtggcgtta tccaacgcct ggactatctg cataaactgg
     8581 gcgttgatgc catctggcta accccctttt atgtctctcc ccaggtcgat aacggttacg
     8641 atgtagcgaa ctatacggcg attgatccca cctacggcac gctggacgat tttgacgaac
     8701 tggtgacgca ggcaaaatcg cgcgggattc gtatcattct cgatatggtg tttaaccata
     8761 cctctaccca acatgcctgg tttcgcgagg cgctgaacaa agaaagccct taccgccagt
     8821 tttatatctg gcgcgatggc gaaccagaaa cgccaccgaa caactggcgt tcaaaatttg
     8881 gcggtagtgc gtggcgctgg catgcggaaa gcgaacagta ctatttgcat ctctttgcac
     8941 cagaacaggc ggatctcaac tgggagaatc cagcggtacg cgcagagctg aaaaaagtct
     9001 gtgaattctg ggccgatcgt ggggtcgacg ggttgcgcct ggatgtggtg aatctgatct
     9061 ccaaagaccc gcgtttccct gaagacctgg atggcgacgg gcgtcgcttc tacaccgacg
     9121 ggccacgagc acacgagttt ttgcacgaga tgaaccgcga tgtgtttacg ccacgcgggt
     9181 taatgaccgt aggtgaaatg tcctccacca gccttgagca ttgccagcga tacgcggcac
     9241 tgacaggcag tgaattgtcg atgaccttta attttcatca cctgaaggtc gattatcccg
     9301 gtggtgaaaa atggacgctg gctaaacctg actttgtggc gttgaaaaca ttgttccgcc
     9361 actggcaaca aggaatgcac aacgtggcat ggaatgcctt gttctggtgt aaccacgatc
     9421 agccgcgcat tgtttctcgc tttggtgatg aaggtgaata ccgcgtgcct gcggcaaaaa
     9481 tgctggcgat ggtgcagcat ggcatgcagg gaacgccgta tatctaccag ggcgaagaga
     9541 ttggcatgac caacccgcat ttcacgcgca ttactgacta tcgcgacgtg gagagcctca
     9601 atatgtttgc cgagctgcgc aacgatggtc gtgatgccga cgagttattg gcaatcctcg
     9661 ccagtaaatc ccgtgacaac agccgcacgc ccatgcaatg gagcaacggc gataatgccg
     9721 ggtttacggc tggcgaaccg tggattggcc tgggcgataa ctatcaacaa atcaacgtag
     9781 aagccgcgct ggccgatgac tcctcggtgt tttacaccta ccaaaagtta atcgcactgc
     9841 gtaagcaaga agccatcctg acatggggca attaccagga tctgctgcca aacagccctg
     9901 tattgtggtg ctatcgccgt gaatggaagg ggcaaacctt gctggtcatt gccaacctta
     9961 gccgtgagat ccaaccctgg cagccagggc aaatgcgcgg caactggcag cttgtgatgc
    10021 ataactacga agaagcctca ccacaaccct gtgccatgaa tttacggcct tttgaggctg
    10081 tctggtggtt acagaagtaa atcttcccta agccccggta atgccggtca ttccggggtt
    10141 ttgtctgtca gcatgttgtt tttgttgatt tttcaaccag caaattcatt aaaaaattta
    10201 cataacgctg tagcgcccgt catccgtacg ctctgctttt tactttgagc tacatcaaaa
    10261 aaagttaaaa catccttgat gcaaagcact atatatagac tttaaaatgc gtcccaaccc
    10321 aatatgttgt attaatcgac tataattgct actacagctc cccacgaaaa aggtgcggcg
    10381 ttgtggataa gcggatggcg attgcggaaa gcaccggaaa acgaaacgaa aaaaccggaa
    10441 aacgcctttc ccaatttctg tggataacct gttcttaaaa atatggagcg atcatgacac
    10501 cgcatgtgat gaaacgagac ggctgcaaag tgccgtttaa atcagagcgc atcaaagaag
    10561 cgattctgcg tgcagctaaa gcagcggaag tcgatgatgc cgattattgc gccactgttg
    10621 ccgcggttgt cagcgagcag atgcagggcc gcaaccaggt ggatatcaac gagatccaga
    10681 ccgcagttga aaaccagctg atgtcgggtc catacaaaca actggctcgt gcttacatcg
    10741 agtaccgtca cgatcgcgac attgaacgtg aaaaacgcgg tcgcctgaac caggagatcc
    10801 gtggtctggt cgagcagacc aacgcctcgt tactcaacga aaacgccaac aaagacagca
    10861 aggtgattcc aacccagcgc gacctgctgg ccgggatcgt ggctaaacac tatgcacgcc
    10921 agcacctgct gccgcgtgac gtggtgcagg cacatgagcg tggcgatatt cactatcacg
    10981 atctcgatta ctcgccgttc ttcccgatgt tcaactgcat gttgatcgac ctgaaaggca
    11041 tgctgaccca gggctttaaa atggggaacg ccgagattga accgccgaag tcgatctcta
    11101 cggcaaccgc ggtaactgcg cagattattg ctcaggtcgc cagccatatt tatggcggca
    11161 ccaccattaa ccgtatcgat gaagtgctgg cgccgtttgt taccgccagc tacaacaaac
    11221 atcgcaagac cgcagaagag tggaacatcc cggacgccga aggctacgct aactctcgta
    11281 ccatcaaaga gtgctacgat gccttccagt cgctggagta cgaagtaaac accctgcaca
    11341 ccgccaacgg tcagacgccg tttgtaacct ttggttttgg tctgggcacc agcagggaat
    11401 cgcgcctgat tcaggaatcg atcctgcgta accgtatcgc aggcctcggt aaaaaccgta
    11461 aaactgcggt gttcccgaaa ctggtgtttg cgattcgcga tggcctgaac cataaaaaag
    11521 gcgatccgaa ctacgacatc aaacagctgg cgctggagtg cgcaagcaag cgcatgtatc
    11581 cggatatcct gaactacgat caggtagtga aagtcaccgg ttcgtttaaa accccgatgg
    11641 gctgccgcag cttcctcggc gtgtgggaaa atgaaaacgg cgagcagatc cacgatggtc
    11701 gtaacaacct cggcgtgatc agcctgaacc tgccgcgtat tgctctggaa gcaaaaggcg
    11761 atgaagccac cttctggaag ctgctggatg aacgtctggt gctggcacgt aaggcactga
    11821 tgacccgtat cgctcgtctc gaaggcgtga aagcgcgcgt cgccccgatc ctctatatgg
    11881 aaggtgcttg tggcgtacgt ctgaatgctg acgatgatgt ttctgaaatc ttcaaaaacg
    11941 gtcgtgcgtc tatttcgctg ggttacatcg gcatccacga aaccattaac gcgctgttcg
    12001 gcggcgagca tgtttacgac aacgagcagc ttcgcgcgaa aggtatcgcc attgttgaac
    12061 gtctgcgtca ggcagtggat cagtggaaag aagaaacggg ttatggtttc agtctctaca
    12121 gcacgccgag tgaaaacctg tgcgaccgct tctgccgtct cgatactgct gagtttggcg
    12181 tggtgccggg cgtgaccgat aaaggttact acaccaacag tttccacctt gatgtggaga
    12241 agaaggtgaa cccgtacgac aagatcgact ttgaagcgcc ttacccgccg ctggcgaacg
    12301 gtggtttcat ttgctacggc gagtatccaa acattcagca caacctgaaa gcgctggaag
    12361 atgtctggga ttacagctat cagcatgtac cgtattacgg caccaataca ccgattgatg
    12421 agtgctacga gtgtggcttt accggtgagt tcgagtgcac cagcaaaggc ttcacttgcc
    12481 cgaaatgtgg taaccatgac gcctcccgtg tgtcggtaac tcgccgcgtg tgcggatatt
    12541 taggtagccc ggatgcgcgt ccgtttaacg ccggtaagca ggaagaagtt aagcgccgcg
    12601 ttaaacattt ggggaatggg cagataggtt aatcttcact ctgcgttaag caaatgccgg
    12661 atgcggcgta aacgccttat ccggcctaca tttggcaacg gctgtaggcc tgataagacg
    12721 cgacagcgtc gcatcaggca ttgattaccg gatgcagcct acacgcggac tgataagcca
    12781 ggcgaaataa tgaattatca tcagtactat cctgtcgaca tcgtcaacgg ccccggcact
    12841 cgctgcaccc tgtttgtctc cgggtgtgtt catgaatgcc ccggttgcta taacaaaagc
    12901 acatggcggg taaattctgg tcagccattt accaaagcaa tggaagacaa gatcattaaa
    12961 gatctgaatg acactcgtat caaacgccag gggatttccc tctccggcgg cgatccgctg
    13021 catccgcaaa acgtgccgga tattctgaaa ctggtaaaac gcatccgcgc cgagtgtccg
    13081 ggtaaagaca tctgggtgtg gacaggctat aaactcgacg aactcaacgc tgcgcaaatg
    13141 caggttgttg atttgattaa cgtgctggtc gacggcaaat ttgtgcagga tttaaaagac
    13201 ccttccctga tctggcgcgg cagcagcaat caggtggtgc atcatttgcg atgataagtt
    13261 ccctgttacc tgagtgcagg caacagggaa atgaggaatt aacgatactt ctggtgatag
    13321 gcgttgcggg tcgttttcag ttgctctgca gcagcctgcg cttcttttac tttaccttca
    13381 tttgccagct tcagcgcgtc gtcaatctga ccgaccagaa tgtcgaaacc gtggcggaaa
    13441 tctttcattt ccgggctgtc cggtgattta tcttcgagct tcggcggcgt tgctttttgc
    13501 gcatccagcg ctgcggcgcg catcttcgtt aacgcgtctt tgacttgcgt cgcgttatcg
    13561 gctttttcga ccacttttaa attgtcgttg agggtttcca tattgtcttc aagatcagcg
    13621 gcaaacgacg ccgaactgaa taccaacgag gagactgcaa gaatagctaa caggctttta
    13681 cgcattgctc acttcctttt ttatttacac ttcatccttc acactgcctc atcgacagct
    13741 acgctcattc gaccgggtca cgtactacga gtacgctccc cggctctcat tcactttccg
    13801 tctcgatgca gtgcgaatga ttttgtgtaa ttttttagtt tatagcgcgg caggtcgcgc
    13861 cagttttttt actgtccggc gattttcatc tccggcaaca gcacagaacc acactgtata
    13921 ttactgcgtg tttcaatatc gttaccgacg gtgacaatat tgcgccacat atcttttaaa
    13981 ttacctgcga tggtgatttc gctcaccgga tactgaattt cgccattctc tacccagaag
    14041 cccgcagcac cacgggaata atcaccggta atggcactca cgccctggcc catcagctcc
    14101 gtcaccacca gcccggtgcc catctctttg agcatttgtt cgaagcttag cccttgtccg
    14161 gcaatccgcc agttgtgaat accgcccgca tgtccggtgc ttttcagccc aagttttcgc
    14221 gccgaatagc tggtcagcag ccactgagtc aggatgccat ctttaataat atcgcgacgc
    14281 tcggtgcgca caccttcgct gtcgaatggc gtcgacgcca gccctttcag cagatgcgga
    14341 tgctcttcaa tggtcagcca gtccggcaga atttgtttac ccagcgaatc cagcaggaag
    14401 gtagatttac gataaaccga tccacccgct atcgccccca ccagatgccc aaaaagccca
    14461 gttgccactt cattggcaaa aatgactggc gctttcatgg tggagagttt acgcggtaac
    14521 aggcgcgata aagtacggcg agcacagtcg gccccaaccc actctggcgt ttgcagatcg
    14581 ctcatcgcgc gcccaatggt gtaggcgtaa tcacgctcca tatcgccatt ttcttcggca
    14641 attacacagc tggagagcga atgacgcgtt gagcagtaac cctgcaacat gccgtggctg
    14701 ttgccaaaaa ctttgacacc gtagtggctg ttaaagctgc caccttcggt attggtgata
    14761 cgtttatccg cctgtaatgc tgcctgttct gcgcgggccg ccagttcaat ggcttcatcc
    14821 ggggaaactt ccgcagggtg gaacaagtcg agatctggtg cgtcaaaggc cagcagctct
    14881 ttgtctgcca ggccggcaca gggatctggc gaggtataac gggcaatatc cagcgccgcc
    14941 tgtacagtac gggcaatggc ctgcgggctt aaatcggtgg atgatgcgct acctttgcgg
    15001 ttctggtgat aaacagtgat ccccagcgcg ccatcgctat tgaattcaac attctccact
    15061 tcaccataac gcgtgcttac gctaatgccg gtggtcttgc tgacggcaac ttccgcaccg
    15121 tccgatttgc ctgaggccaa ctccagcgca gtggaaactg cttcttccag aatcttgcgc
    15181 tgcgcttcaa cttgagagat tactttcatt gcaagtgcca taatgtagag agtttctctg
    15241 aagtctaaca gagaaccgtt tttcagtgcg catcttaact ggtaacatta gcctcttttt
    15301 ttaaggagcc tgagatgact aagcagcccg aagactggct cgacgacgtt cccggtgatg
    15361 acatcgaaga cgaagacgat gaaattatct gggtcagtaa aagtgaaatt aaacgtgatg
    15421 ccgaggagct aaaacgcctt ggcgcggaaa ttgttgatct ggggaaaaac gcgctggata
    15481 agatcccgtt agatgcggat ctgcgcgcgg ctattgagct cgcccagcgt attaagatgg
    15541 aaggtcgccg ccgccagttg cagctcatcg gtaagatgct gcgccagcgc gacgtagagc
    15601 ctattcgtca ggcgctggat aagctgaaaa accgtcacaa ccagcaggtg gtgctgttcc
    15661 ataaactgga aaacctgcgc gatcgtctga tcgatcaagg tgatgacgcc atcgctgaag
    15721 tattgaatct gtggcccgat gccgatcgtc agcaactgcg tacactgatc cgcaacgcga
    15781 agaaagagaa agaagggaat aagccgccga aatccgcacg ccagattttc cagtatctgc
    15841 gcgaactggc ggagaatgaa gggtaatttt cgttgttgat gtgagtatgg tttgccggat
    15901 gcggcgtaaa cgccttatcc ggcaatgcta tatcaggctg aagccgaatt actgcgcagc
    15961 ttccgccttc ttcgccagac catccagcag tttctgatgg atcccaccaa aaccgccgtt
    16021 gctcatcacc agaatatggt cgccaggctg agcggttttc accaccatat ctgccagcgt
    16081 atccacatcg ccactccagt gtgcaggctg aacgcaggct tctgccactt ctgccacctg
    16141 ccacggaata tgcgccggtt gcagcaggaa gacttcatcg gcacgaccta atgaaggtgc
    16201 cagatcgtct ttgcagatcc ccattttcat ggtattcgag cgcggttcca gcacagcaat
    16261 aatgcgcgcc gtaccaccaa ctttgccacg cagcgccgcc agcgttgcca gaatcgccgt
    16321 cgggtgatgg gcaaaatcgt catataccgt gacgccattc gcttcaccac gcaactccag
    16381 acggcgacga gcattaataa acgaacccag cgcgttagcg gcatctgccg acgctacacc
    16441 aacatggcga gccgctgcaa tcgccatcag gccattgtgc atattatgtt cgcctaccag
    16501 cgaccatttc acttcgccca ctttttcgcc atccagcaaa acttcccatt cggaagcatc
    16561 ggtggtcagc tttttcgcct gccagtgacc ctgctcaccc accagctcct gctcgctcca
    16621 gcagcccatc gccatggtct gtttcaggtt gatgtcattt tccggccaga taatacggcc
    16681 ctgccccgga acgatacgca ccagatggtg gaactgtttc tggatcgctt tcaggtcgtc
    16741 aaagatatcg gcgtgatcga actcaaggtt gttgaggatc agcgtacgcg ggcagtaatg
    16801 gacaaattta gagcgtttgt cgaagaaggc gcagtcgtac tcatccgctt cgataacaaa
    16861 gaagtcgctt tcgcccagac gcgccgaaac ctcaaagttc cccggcacac cgccgatcac
    16921 aaatcccggt ttgtaaccac actgttccag aatccaggtc gccattcccg cggtggtggt
    16981 tttgccgtgt gtaccggcaa cggccagcac ccagcggtcg cgcagcacaa aatcgtgcag
    17041 ccactgtgga cctgacatat aagggatgtt tttttccagt accgcttcca cacacggatt
    17101 tccacgggtc atggcgttgc caataatcac cagatccggc tgcggctcga gctggctggc
    17161 atcgtaaccc tgaatcagct caatgccttg cttctcaagt aaggtgctca tcggcggata
    17221 cacattggcg tccgaacccg ttacttcatg gcctaactgg cgcgccagca tcgccagacc
    17281 gcccataaat gtgccacaaa ttcctaaaat atgaatgcgc atacgtcact atcctttttt
    17341 aatctgccgc tcattttacg catatgtcgg gcaagtgaga aacgcatttc aggataatcc
    17401 gcaatttgct ggcgcgattc acctgagcgc aacattgaga ttatttgtta agattgttgc
    17461 ggtcgcttta ctccataaac attgcaggga aagttttatg aaaacgttag gtgaatttat
    17521 tgtcgaaaag cagcacgagt tttctcatgc taccggtgag ctcactgctt tgctgtcggc
    17581 aataaaactg ggcgccaaga ttatccatcg cgatatcaac aaagcaggac tggttgatat
    17641 cctgggtgcc agcggtgctg agaacgcgca gggcgaggtt cagcaaaaac tcgacttgtt
    17701 cgctaatgaa aaactgaaag ccgcactgaa agcacgcgat atcgttgcgg ggattgcctc
    17761 tgaagaagaa gatgagattg tcgtctttga aggctgtgaa tacgcaaaat acgtggtgct
    17821 gatggacccc ctggatggct cgtccaacat cgatgttaac gtctctgtcg gtaccatttt
    17881 ctccatctac cgccgcgtta cgcctgttgg cacgccggta acggaagaag atttcctcca
    17941 gcctggtaac aaacaggttg cggcaggtta cgtggtatac ggctcctcta ccatgctggt
    18001 ttacaccacc ggatgcggtg ttcacgcctt tacttacgat ccttcgctgg gcgttttctg
    18061 cctgtgccag gaacggatgc gcttcccgga gaaaggcaaa acctactcca tcaacgaagg
    18121 aaactacatt aagtttccga acggggtgaa gaagtacatt aaattctgcc aggaagaaga
    18181 taaatccacc aatcgcccgt atacctcgcg ttatatcggt tcactggttg cggatttcca
    18241 ccgtaacctg ctgaaaggcg gtatttatct ctacccaagc accgccagcc acccggacgg
    18301 caaactgcgt ttgctttatg agtgcaaccc gatggcattc ctggcggaac aagcgggcgg
    18361 taaagcgagc gatggcaaag agcgtattct ggatatcatc ccggaaaccc tgcaccagcg
    18421 ccgttcgttc tttgtcggca acgaccatat ggttgaagat gtcgaacgct ttatccgtga
    18481 gttcccggac gcgtaatctc ctgcgcctct ccacacggag aggcgttttt attgctgcgc
    18541 aatgttgact cttgtcactg gcgaactctg acgattctcc cacagcaccg ttaatccacg
    18601 ctgtaatgct ataaaaataa acaacaaaat accgatggcg attttcgtcc accaggaact
    18661 cagcgtgcca tcaaagttta tgtaagtctg aatcagtccc tgaatcgcca cgccaaaaag
    18721 cgtccctaat accgttccaa cgccaccgct caaaagcgta ccgccaatta ccactgaggc
    18781 gatagcatcc agctccacac ctacgcccgc cagcgcatat ccggcctggg tataaatcga
    18841 gaagacaatc cccgccagcg ttgccagtcc ggtggagagc atataaatgc gaatagtggt
    18901 gctgcgagtg gaaatcccca tcagattcgc cgacgttgcg ttgccgccaa tggcgtatac
    18961 ctgattacca aaacgggtac gatgcgcgag gaatatgccg ataaccacca ccgccaacat
    19021 cagcagtccc atcgcactta agcgaccgcc gccagggatt ttccacgcaa ggcttgagag
    19081 cgtgtcataa atgggatggt ttatcggaat cgactcttcc gaaacgagat agctgacgcc
    19141 gcgcaaaaag aacatccccg caagcgtaat gatgaacgcc gggatcttca gggcgtcgat
    19201 cagaagcccc ataaatgcgc cgaaggcaca gcccatcacc agcaccagcg gaaacgccag
    19261 cagcggcgag aggccgaaat cgccaatcac ttttgccaga aacacgccgg taaaggcgat
    19321 caccgaaccg acggagagat cgatcccacc ggagaggatc acaaaggtca tgccaacggc
    19381 aatgatccca agaaaggcgt tatcggtcag gatattgcag atcactcttg tggaagcaaa
    19441 accaggaaac tgcgtcaggc agtagagata gcccagcaca aagacgccga tggtgatcat
    19501 aagcggcaag ttacgtttta tcacggctac gcactccttt aatcagactg ataaagcgtt
    19561 gcgactgaac aatcagcaca caaagcacca ccaccgcttt caccacctgg ttcatctctg
    19621 gcggaaagcc cgaaagcaaa attccggtat tcattccctg aataatcagc gccccaactc
    19681 ccgaaagcag caggttaaag cgaccgccca tcagcgatcc accgccaatc accaccgcga
    19741 gaatggcatc cagctccagc cataatccgg cgttgttggc atcggcaccg cgaatatccg
    19801 ccgccacgat aatgcccgca atcgccgcac atagcccgct caacacgtag gtgagcatga
    19861 cgatgattcg tgtgttgact ccggcatttt ttgccgcccg aatgttgata ccaacggctt
    19921 cgataaacat ccccagcgcc gttttgcggg tcaacagcca gaacaggaga agcgtcagca
    19981 ccgcgataat gaccggcgtt ggcaggaaca acagcgatcc gctgccaaac catgagagat
    20041 ccggcgagtt aaacgtgacg atctgcccgg cggtgatcag ttgcgccacg ccgcgcccgg
    20101 cgaccatcag gatcagggtg gcgacaaacg gctgaatttt gaggatcgct accagtatgc
    20161 cgttccacaa tcccgctagg atgccggtgc ccagggcgct taacaaaaca atcggcaggc
    20221 tgaatcccgc gaccgtcatc gcagccgttg tggctccggc gatagccatc accgccccca
    20281 cagagagatc gataccaccg gtggcgatca ccagcgtcat accaatcgcc agtaacgcaa
    20341 cgggagctgc acggttaaga atgtcgatag ggctaccgaa caaacgccca tcctgaagca
    20401 ccacctgcca gaaatgcggg gctaccaggc tatcgaccag cagcaccagc aacagtgccg
    20461 ccagctgcgg catccccgtc ggccagtgaa aacgcctttt tggcggcgta gtgtcaggga
    20521 gagattgagg catcacactg ttctccttac gccgcaatgg cgttcatgat cgccggaacg
    20581 gaaagctgtg ccagcgggat ctccgccacc tgtttgcgat cgcgcatgat gatcacccgg
    20641 tcggcatagc ccaccagctc ttccagttcg gaggagatca ccagcagcgc cagaccgtca
    20701 gcacacagcg tttcaatcag gcggatgatc tcggcgtggg caccaacatc aatgccacgg
    20761 gttggctcat caaggatcag aaattgcggt cgggtcagta gccaacgtga aagcagcact
    20821 ttttgctgat tgccaccgga gagaaattca atcggttgtt cagttgaagg tgtgcgaatg
    20881 ccaagctggc ggataaagcg ttcggcaatc tcttgctgct ctttgcggga aatgggtcgt
    20941 agccagccgc gctgggcctg gagagcgagg atgatatttt cccgcaccga ggcggcggcg
    21001 atgatgccat cggttttcct gtcttccggg cagaatccga tgcccagtac tgaagcctga
    21061 tgtggcgatc gcagggtttg cggtttgcct ttgatcaacg ccgtgccgct gtcagcaggt
    21121 ttgataccga agatcacttc ggcggtttcg gtacgtccgg atcccagcaa gccagccaga
    21181 ccgacgatct cgccggggcg cacttcgaga tcaaacggtg ctatcgttcc ttttttgccg
    21241 taatttttga acgcggcaac gggtttgtcg ctcaacaatg ttcgcccggc acgctgtagc
    21301 acgtgggtat ccagctcgcg ccccagcatc atttttacca gttcgatctg cggtagctcg
    21361 cgcgtttccc gacagcctac gaaactgccg ttgcgtaaga cagtgatccg atcgctgacc
    21421 tgatagacct gatcgagaaa gtgagtgaca aagatcaggc tgacgccgcg atcgcgcaac
    21481 tgacgcatca ggccaaataa tagctccact tcctgggtgt cgaggctggc ggtgggttca
    21541 tcgaggatca gcactttggc ggagagatcg atagcccggc aaatggcgac gatttgctgc
    21601 atcgcgactg aaaagcggtt aagcggttcg cgcacgtcga ggaagaaacc gtaagatgcc
    21661 atcagttcgg tggcgcgctt ttccatctct ttacggcgta gaaggccgaa acgtttgggt
    21721 tcgcgaccta tgaatagatt atcagcaacc gacatattgg gtagcaggtt gacttcctga
    21781 tagacggtgc caataccgag ttgttgtgca tgggcggtat ttttcggtga gatagcctgg
    21841 ccttccagcc agatggtgcc gcgatcggcg tggtatacac cagttaatgc tttgattagc
    21901 gttgattttc ccgccccgtt ttcaccaagc agcgccatga tttcgccacg gcgcaggctg
    21961 aaatcaacgt tgtctaacgc tttgacgccg gggaaaaatt tacttaatcc ttcggtgcgg
    22021 aggatctcct ggtgttggtc ggtcgtcatg gtttcccccg gtgtaaagcg tgctcccagt
    22081 aggcatgata agacgcggta agcgtcgcat caggcttcag tgcaccatcg ccggatgcgg
    22141 cgcgagcgcc ttatccggcc tacgaatcgg catcaggcat agcaaccctc aataccccat
    22201 atttttcttc ttctctaact cttcttttgc ggtatcaggc aggtaaaggg tggatttggt
    22261 taacgtcagc ttttcaggca tggtgccgtc ttttttgtat ttctccagcg catcgaaagc
    22321 gggtcccgcc atgttcggcg tcagttcaac actggcgttc gcttcgccat ccatcatcgc
    22381 tttgtagatg tccggtacac cgtcaatgga acccgtgagg atatctttgc ccggtttcag
    22441 gcccgcttct ttaattgcct gaattgcacc aatcaccatg tcgtcgttat gggcgtaaac
    22501 catgcagatg tttttgccgt tgttttccgc tttgataaag ctctccatga cttctttgcc
    22561 tttactgcgg gtgaagtcac ctgactgcga gcggataatt ttgatatttg gcgcattctt
    22621 aatggcttcg gcaaagcctt tcttacggtc aatggcgacg ctggccccaa cggtgccctg
    22681 cagctccacc acgttgcatg gtttgccatt cacttctttt accagccagt caccgatcaa
    22741 cttaccttcg aggatgttgt cggcagtaac ggtggtcata tagagagatt tgtctttcac
    22801 atcgatggaa cggtcgagca agaagaccgg gatttcggca tctttcgcct cttttaatac
    22861 cggttcccaa cccgtcgcaa ccactggagc gataaagatc gcatccaccc cttgtgcaac
    22921 gaaggaacgt accgctttaa tctggttttc ctgcttttgc tgaccatcgg caattttcaa
    22981 cgtaattccg cgtttttcgg cctcactttt cgccacattg gtttctgcgg cgcgccagcc
    23041 ggattccgat ccgacctgcg aaaatcctcc ggttaatgga gctgccaacg ccatagacga
    23101 catggctgcc gagactgcag agactacaag taagcgtttc cacatagggt tgtcctcgtc
    23161 ggggtaatta ttattggtga aaagatgttc gcgaaaaaac tatagacaat tcgttatgta
    23221 acggattgcg ttacatcaca cttcaaaaaa gtaaataaaa cgttaatcac aagtttgtaa
    23281 tcgctttcat ctcactatga aaaatgcggc tacggttatg gattttcctg ctctgtatac
    23341 cgtcttaaaa ctggcgaaaa aggaaaatga agacgaaaac aagcaaagac attcggcgcg
    23401 agttggctat aatactcggc acttgtttgc cacatatttt taaaggaaac agacatgagc
    23461 ttactcaacg tccctgcggg taaagatctg ccggaagaca tctacgttgt tattgagatc
    23521 ccggctaacg cagatccgat caaatacgaa atcgacaaag agagcggcgc actgttcgtt
    23581 gaccgcttca tgtccaccgc gatgttctat ccgtgcaact acggttacat caaccacacc
    23641 ctgtctctgg acggtgaccc ggttgacgta ctggtcccga ctccgtaccc gctgcagcct
    23701 ggttctgtga tccgttgccg tccggttggc gttctgaaaa tgaccgacga agccggtgaa
    23761 gatgcgaaac tggttgcggt tccgcacagc aagctgagca aagaatacga tcacattaaa
    23821 gacgtaaacg atctgcctga actgctgaaa gcgcaaatcg ctcacttctt cgagcactac
    23881 aaagacctcg aaaaaggcaa gtgggtgaaa gttgaaggtt gggaaaacgc agaagccgct
    23941 aaagctgaaa tcgttgcttc cttcgagcgc gcaaagaata aataagttct tctagcgcaa
    24001 taaccctgaa cgccgggctt cggttagtaa gggtttttta tgcccgcgat aaataaactc
    24061 tctattccac catcattatt ctcagcggtt gcaaggcttg aacggtaaga acaagcaaac
    24121 ccgaccacca ttttgctgtt catagccact tgctggaagt tagccgacct cactcatact
    24181 caccgttcaa agcgtgcccc cgttttaccc ctgcacattt gtgaacgcca gcagagtttt
    24241 ttatgcgatt gcagatatgg aaagcggctc ggagagaaaa gcaaaagatg gaaaagttac
    24301 aaaaaaaaat cgcgagatac atcgcataaa acaacgccac ccgaaggtgg cgtgctttta
    24361 ttattactta tccctgtcta accagtcgcc gctttcaatt agctttaatc catcgacggg
    24421 tcgttgataa acgtacatcc atgcactccc gtacggcgtc tgaatcaact ggcgcgcgta
    24481 ttcaccgccc ctggtgcgca aggcatcaag ttcggccagc gtggcgttgt caatacgata
    24541 aacttcaccg tgtaccgttc cgttccccgg aactgcgcct ggatagtggc ccaggctata
    24601 caactggtag ttatcgatac tgaaatcgcc cagtaactgg gcattggtca tccagtgact
    24661 gttgccttgt ttgtggcgta aactgccgta gacaaatatt cgcattgcta aaactcgaac
    24721 tgatagagca aatccagtgc ctggtctaca ccagacacgg cttccagata tagcttaggc
    24781 atcaggcgat aacgtaacgt gagtgttgct atagagtcaa atatacccac gccgtatttc
    24841 acttgcagac ctggcaatac atagccgctg accactacct gggaggagtc gcctactccc
    24901 tgggtgtcga gcgctaaatt gcttacgcca aacgtctcgc cgattttacc cacaatctgg
    24961 ccactttgcg caacccccag accaatcagc atcgaggtca ttgccgcact gtcgctctga
    25021 tcgctctcca gcccttgtcc acgtagcaaa taagacaatg cagcttgttg cgacatcgcc
    25081 gggtcagaga agatctccgc tttcggttcg tccgccagac cagtgacgcg aactccggca
    25141 attacgtcgt cttctgtagc atccgggtta cgaatagctt caatattaag atacggttga
    25201 tccggcggac cagagaacag taactcaccc ttacgcacaa tcagatcctg accataggca
    25261 tggaagcgcc cttcagggat gttgatctgc ccgttcaggc ccagcccttg tttgtcctgt
    25321 acgacgttaa gatcgcccgt cagccgcgct ttcaggccaa aggcgtcaat gcgcacattg
    25381 ttgccaacgt ggacaatcag gttactgtta atcggaatcg acgccgtttt cggctcttcc
    25441 ggttgcaggt tatcgttaag catcaccaca tcgctggaga cgcctactgc gctttccggc
    25501 agatcgtgca ccacgatgcg cgcccacggg acatccacgc gaccatcgag ggtaaacagg
    25561 tttggtgtag cctcgaatac aacatctggc gatacatcca ttcgtaccat cggcggcacg
    25621 gtgatccgca ctttactgcc tttcgccgtt actcgtgccc gccagttttc aatttggctc
    25681 cagtcggcat caccgttcag gtagatttca ccctgctggg tccgtactgt accggcaagc
    25741 gtcgagcgca taccgttaaa gttgaccgca agctggctcg gctgcatatc aaacggcata
    25801 aagttgccgt cgatatccac acccgtaacc tgaagctgac caaacaactg cgggctttgc
    25861 acatcaccac ccagacgcaa gttggcactc accatccccg ctgctttttc cccacgggta
    25921 aagatggggt ttatcatcgc aaggttgaag ttacggatat tgacgttgcc accaagatta
    25981 cggcggcctt gcggatcggt cacctgcacc tgtccatcaa actggccgtt attggtcagg
    26041 cggatggtcc agcccaattc ggcacggttg ttacgcaatt ccgccgtcag attcagtgtc
    26101 tgaaacgcca ccggcagcgc cgcatcgttg acggtttgcg ttacctgcac gttacgcccc
    26161 gaaagggtga tactgccctg cggcagcccc tctttcgtgg tgtcccaggc aacatccgct
    26221 ttacccgtga agataccgct ggcctgagtg gtttctggca taaacggttt cagcatggcg
    26281 aggtcgaagc ggttgagatt caccaccgca cgcccttcgg cccccgcatc gatagtttgc
    26341 ggcacgcaca gttccgcatt cgggttaagc cagcagtgtg gcccgatgct gattttttgc
    26401 tccttattgc ggtaatccag cgcaatatcg cgggtcagcg accacgggcc gaccggcgtc
    26461 tggaaacggg tattgctaag agttcccttc cagcgttctt ctttgcgatc aaaacttcct
    26521 gccagattaa gctgcccgga gacaggctcg ccctgaatcc gcaactgtag ctcgtgctgc
    26581 ttttcgctgc ctttggcatt cagggtgacg aggttgatat ttacatccgg ctgcgaaatt
    26641 tgctcaacgc gtacgtcgag tttcccggcg atctgatcgg tggatttaat gtcgccttcc
    26701 acgcgaacct gcgccacgga aagttcctgc cagcgcaggc cgcgcgcggt gatatctgcc
    26761 agtagttgtg gcgcttccac cgtgccgcgt actttcacca gccctttcgc tgtaccgcca
    26821 agccccggca gcgcgttatc cagccccggc gcgttgatgg tggcatcaag attgagatct
    26881 tttaccccca gctcgccttt cacttcggca ctgtttggcc cgagttccag atgaagccca
    26941 gggatcatcc actgcatata actgttgcct ttcagcgtgc cgtcaacgtt cactttgttc
    27001 tgtttgacgt taccggtcag cttcagttct ggcacctcca tctgccaggt gccgccgtac
    27061 aggctaccgc gggttttaat caagccattg agtttcgacg gccactccgg gaactctttg
    27121 gcggtgttaa tgccattaag cgttagctca ccgcgccaac taatggcctg ctgccagtcg
    27181 agcaacgcct tgagttcagt tttcccttcc agcgccgcga cggtgagttt gtcgagattg
    27241 acctgctgtt cattaccttt ggcatcaagg gtaatggtgg ctggcgggat ctcctgtccc
    27301 ttcactgccg tacgcataga gagcgtgtaa tcggtcattt tgccggtaag tttcagtttc
    27361 agatcatccg cctgatactg cttctcacca gtgaacggcc agtaaagctg tttgctgttc
    27421 acttccacgt tgagtggcaa tccggcttcc gccagtcgcg tctgggcgcg taaatccata
    27481 tccaccggac cggaaaggtt aacgcctatc tccagctgtt cgcgcagcgc gccgcccact
    27541 ttcagcttca ccttttcacc tttcaacggc tccacgttca gtgtgctgtt gaggatgata
    27601 tccaccggcc agttgtctga cagctgcgcc gtgccgctgg cgttgacgat cccctggttg
    27661 gaatcgatat ccagggcgtc cagtttagta ttgccgtcaa tgctgctcac tttcagcagc
    27721 atggtgcgca cggtgatgtc cgtgtcgccc gtcacgcgca gctgctcgcc cttaaactct
    27781 tcaatgttca ggttaagcgg caaatgcacg tcggtcattt ccggcaatac cgggcgagaa
    27841 aagagatctt tcagcgtttc gccgagcggc ttttcatccg gctgcggatt ttcaatcttc
    27901 ggttcgacga cctcttcctg cgccacttcc gccactttcg gcagagcaat cagcaggcct
    27961 ttcagcgacg tcggtttcag ggtcagggtt ttctcctgcc agttcaggcc ggaggtgaag
    28021 tccatcaccg acaccgtggt gtcatcaatc ttgatgttga cgttatccag cgccacccgt
    28081 gtcagggtga tgggatacgg cgtggagaga tccagcggac cgctatcttc ctcttcttca
    28141 acctgttcag aaggaggcat ttttttactg tcgatgttga cctgaatgtc tttcagcgcc
    28201 aggtcattaa tacaaacgct actgttccac aggcactcaa gaccgaccgc cagatgcaga
    28261 ttacccgctt ttaccgccac gcctggctgc tcataacgaa cgtcagacaa ggtgagatcg
    28321 cgccagccgc cggtgacgtt gccaatatcc agtcctggca cccagcgatc cgccgcttta
    28381 aatacaagat gcaggccgct ggtggtgccc accagaaacg ccaccgatcc cagcaacagt
    28441 aagataacga taaccacgcc gaggctgatt tttttccata aactcataat tctggcccca
    28501 gaccgatgta aaactgtaac ccgtgttcgt ctttatccgc gaccggtacg gcaaaatcga
    28561 gtttgattgg cccgaccggc gattcccagc gtacgccgcc cccggtaccg gttttaaagt
    28621 cgctgcggcg aatatcgctt accgcttcgc cactatcgac aaacaccgcg ccccaccatt
    28681 ttccggtcac gttgtactgg tattccagcg atccggttat cagcttcgag gcccctttca
    28741 ggtcaccgtt ggcgtatttc ggggcgatag atttatattt gtagccgcga atactacgat
    28801 cgcctccggc gaagaaacgc agatccggcg gtactttgtc gaaatcaccg gtttcaatcc
    28861 agcccagcgt gccgcgtgtc acaaaacgat ggcgatcgta cagtgtgcgg atccagacgt
    28921 tctgcgcctg gaaaacggag aaatcaacat ctgaacccca ggccgtgttg gagtagtcga
    28981 tagagtagcg ttgcgagtcg ccccaggttg gcatcaggcc accacgagaa cgcgtgcggc
    29041 taatcatcac cccaggataa aacagcatcg tggtgttggt aatttcaccc tgggtaaagt
    29101 ggtcgaggct ccagcgcagg ttaatggcac gctgccagtc gctggagaga tcccagtagc
    29161 gagaagccac cagcgtagtg gagtcagatt cggtatcgtt caggtcagtg cgcttaaaac
    29221 cgccctgcac caaataatat tgttccagtg gattcttcag cagcggcatt ttatagctga
    29281 agtcgaggat ctgttccggc gcggaaatac tggtactggt ggtcagactg tgaccataag
    29341 agttcatcca cggctttttc cacgtcgctt tcacgcgcgg tcccacgtcc gtagagtaac
    29401 cgaccccggt ttcgatggtg ttttctgttc gcggcgaaac cacgcccgtc aatggtaata
    29461 ctttcgtttc gcgcgcttta tcaaattgcg gtgccactac caccgaatta aaccagccgg
    29521 tagcagaaag tcggcggttc agttccgcca gatctttcga ttcatactca tcgccctctt
    29581 taaacggcac cagattttgc aggtattcat cgcggatttg tgatccttca aaggtcacat
    29641 gcccaaagcg gtaacgttcg ccactgttat aatcaatatc ccagaaggct ttatgcaggc
    29701 cgagcgcaat gcccagctgc gctttggtaa attcgctatc gaaataacct ttacgcaacg
    29761 caatactggt taaggacttt ttgaaatttt cataatcgcc ctggttcagt accgtaccaa
    29821 tagccgggcg agtatcgagc aatttcaaat agtctttatc ggtccgcgcg ccgccgcgca
    29881 ataccacatc ggtgccgcca attaacaccg gtacacctgg cgtgactttg gcgatcaata
    29941 cctgccgccc tttctttggc ggtggacgga gatcaaattc aatggtcggc tggtaataac
    30001 ccagcgcttt cagaccttca cggatggcat catcgacgcg tgcgcgaaag cgacggtctg
    30061 gcgtcacttc atcactttca atcgtagaaa gctgcgcacg aacgttcttt tccagctgtc
    30121 ccgataaccc ctcgacctgt agacggacgt tcgcggcgac ggcagatccg cttaagcaga
    30181 gtaagcttac acagcataac tgtcggatat agcgcacatt ttctcctgaa tatccttttt
    30241 tcctacccct ggaacgccgt taaacggctt aacaaaaatc cagtaatatg gattaaaaag
    30301 cagactaaac cccaaatatt tcttatgttt tactttagac ctattcacgg tggttattgt
    30361 gtgcaaatac gcctcttgtt acaaccttaa ccccaatgac cgattttcgg gagagcgaca
    30421 ccatgagttt atttgataaa aagcatctgg tttcccccgc cgatgccctg cctggacgta
    30481 acaccccgat gcccgtagcc acgctgcatg cggtcaacgg tcactcaatg accaatgtac
    30541 ctgacggaat ggagattgcc atttttgcga tgggttgttt ctggggtgtg gagcgtctgt
    30601 tctggcagtt acccggcgtt tacagcaccg ccgcaggcta taccggcggc tatacgccaa
    30661 atccgactta tcgggaagtg tgctccggtg atacgggtca tgccgaagcg gtacgcattg
    30721 tttacgatcc ttccgtcatc agctatgagc agttgctaca ggtattttgg gagaatcacg
    30781 atcccgccca gggaatgcgt cagggcaatg accacggcac gcagtatcgt tcagcgattt
    30841 atccgctgac ccctgaacag gatgccgcag ctcgcgccag tctggaacgt tttcaggcgg
    30901 cgatgcttgc cgccgatgac gatcgtcaca tcaccacgga aatcgctaac gccacaccgt
    30961 tttattatgc cgaagatgac caccagcaat atctgcataa aaacccatat ggttactgtg
    31021 gaattggcgg aattggcgtc tgtctgccgc cggaagcata gcgttacggg tacaaatgta
    31081 gtttgttgat aaagtgctct ttgtctatgc ctgatgcggc ttaaacgact taccaggcct
    31141 acaaaatcgt ccaaattcaa tatattgcaa ggactgcgta ggcctgatcg gcatagcgca
    31201 tcaggcagta ttgcatttat cagcagtctg aattttaacc ctctggcgac tttacagtac
    31261 cttacgctat actagccact gaaaatgtcg gttcactttc ttcgaatcgg ctttcaatgt
    31321 gtatttcaca caaattaatc aactttccct tccgaggatc tggcctgaaa ggtcggataa
    31381 gatatgttaa acagtatttt agtcatactc tgcttgatcg ctgtaagtgc gttcttctcg
    31441 atgtccgaga tctcgcttgc cgcctcacgc aaaatcaaac ttaaactgct ggctgatgaa
    31501 ggcaatataa atgcccaacg cgttctgaat atgcaggaaa atcccggcat gttctttacc
    31561 gtggtccaaa tcggtctgaa cgcagtggcg attctcggcg gtatcgtcgg tgatgcggca
    31621 ttttctccag cttttcacag cctgttctcc cgctatatgt cggcagagct ctctgagcaa
    31681 ctgagcttta ttctctcttt ctcgttagtg actggcatgt ttatcctgtt tgcggattta
    31741 accccgaaac gcatcggtat gattgcgcca gaagcggtgg ctttgcgtat catcaacccg
    31801 atgcgcttct gcctgtacgt ttgcacccca ctggtgtggt tcttcaacgg cctggcgaac
    31861 atgatcttcc gtattttcaa actgccaatg gtacgtaaag atgacatcac ttctgatgac
    31921 atctacgcgg tagtggaagc cggtgcgctg gcgggcgtgt tacgtaaaca ggaacacgag
    31981 ctgattgaaa acgtctttga gctggaatcc cgtaccgttc cgtcttcaat gacaccgcgt
    32041 gaaaacgtga tttggtttga tctccacgaa gatgagcaaa gcctgaagaa taaggtggcg
    32101 gaacatccgc actctaagtt cctcgtctgt aatgaagata ttgaccacat catcggttat
    32161 gtcgattcta aagacctgct gaaccgcgtg ctggctaacc aaagcctggc actgaacagc
    32221 ggcgtacaaa ttcgcaacac gctgattgtg ccggatacgt taaccctttc agaggcgttg
    32281 gaaagtttta aaaccgcagg tgaagacttc gcggtgatca tgaacgagta cgcgctggtg
    32341 gtggggatca tcaccctcaa cgacgtgatg accacgctga tgggcgatct ggtcggtcag
    32401 gggctggaag agcagattgt cgcccgtgat gagaactcat ggctgattga cggcggcact
    32461 ccgattgacg acgtcatgcg cgtgctggat attgacgaat tcccgcagtc gggcaactac
    32521 gaaaccatcg gcggctttat gatgtttatg ctgcgtaaga tcccgaaacg caccgattcg
    32581 gtgaaattcg ccggctacaa atttgaagtg gtggatatcg ataactaccg catcgaccag
    32641 ctgctggtga cccggatcga cagcaaggcc accgcccttt cgccaaaact gcctgacgct
    32701 aaagataaag aagaaagcgt cgcgtaaccc cccagaaaca tcaacggctc ctgaatcagg
    32761 agccgtctta ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    32821 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    32881 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    32941 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    33001 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    33061 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    33121 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    33181 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    33241 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    33301 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    33361 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    33421 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    33481 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    33541 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    33601 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    33661 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    33721 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    33781 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    33841 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    33901 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    33961 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    34021 aatggtcgac ttatatttct tgcaaatctt ttctttggta atattactat ctgcggccag
    34081 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    34141 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    34201 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    34261 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    34321 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    34381 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    34441 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    34501 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    34561 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccattatc
    34621 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    34681 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    34741 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    34801 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    34861 aataaaatta ttgtgtctga tcctgggtaa catatgacaa ccttatttta tttggttatt
    34921 ttatgtcgcc attaaatatc tcattagaga agtaaactat caaaaaacgc tcattattaa
    34981 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    35041 ctttttaggt tgggagaaat caggatggca gaatcaggaa atacactgtt tctgccatct
    35101 gaatttagta aatagacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    35161 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    35221 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    35281 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    35341 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    35401 ggcgcgcatc gcggacctga atctgcttgc gcacaccgca ctcttctttc caggccttgc
    35461 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    35521 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    35581 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    35641 cgggaggatc ttcttcagaa aggaccggaa tatccggtgt cagcgtacgt aaaccgtcca
    35701 tgataacggt gtgtgcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    35761 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    35821 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    35881 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    35941 aaagcgtaaa attgtggcat tcttcactgt tctataagta agacgtttat tctccctttt
    36001 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    36061 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    36121 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    36181 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    36241 cgtactggtc gataacggcg atttgattca ggggagtccg ctggcagatt acatgtcggc
    36301 gaaaggatta aaaacaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    36361 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    36421 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    36481 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    36541 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    36601 agctaattta tccgggaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    36661 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcacatt ccgggctgtc
    36721 tgccgatccg tataaagtga tggcggaaaa ctcagtttat tacctcagtg aaattccggg
    36781 cgttaacgcc attatgtttg gtcatgctca cgccgttttc ccgggtaaag attttgctga
    36841 tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg
    36901 catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg
    36961 gcaggtgacg caggcgaaag cggaagctcg gccgatttac gacatcgcca ataaaaaatc
    37021 cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg
    37081 ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct
    37141 ggtgcaggac gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca
    37201 ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt
    37261 taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt
    37321 gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc
    37381 cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattga
    37441 tcctaacagc acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt
    37501 tgatgttatt gatggtgtga attatcagat tgatgttacc cagcccgccc gttatgacgg
    37561 cgagtgccag atgattaata ccaatgcgga aaggattaag aacctgacct ttaatggcaa
    37621 gccgattgat ccgaacgcca tgttcctcgt tgccaccaat aactatcgcg cttacggcgg
    37681 caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc
    37741 ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc acccggcggc
    37801 agataacaac tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga
    37861 aacttccccg tcagataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa
    37921 taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa
    37981 cacttctttt tcggcctata aatcatcaat cgcatccggc atttattggc ggatgcgatg
    38041 ctggcgcatc ttatccgccc tacaagtcat acaccgtagg ccgcgcatcc ggcagttatg
    38101 ccgcacgttc atcccgcacc gccaacacct cgggcaaatt caactcaatc cagtccgcca
    38161 gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt gaggctgtat tcaacatgcg
    38221 gtggcaccac cggatacgcg atacggttaa gaaaaccatc ctgttctaac gcctgtaacg
    38281 actgcgcaag catcttttcg ctcaccccgc cgattttgcg ccgcaggtcg ctaaagcgat
    38341 gagtaccttc gcgtagcgcc accagaatca acacccccca acggctggtg acgtgtttca
    38401 acacctcgcg cgacgggcac tgttccgcaa agagattacc ctctttcagt tgttgcgaca
    38461 ggctaacctg actcatttca tacttacctt tttgtacgta cttactaaaa gtaagtttag
    38521 gtgttagcgt atttaaacac aagacaaaac gatggagact tcccatgatc gctattactg
    38581 gtgccactgg ccaacttggt cactatatta ttgaatcctt gatgaaaacg gttcctgcca
    38641 gccaaatagt ggctatcgtt cgtaatccgg caaaagccca ggctctagca gcacaaggca
    38701 ttaccgtgcg tcaggctgac tacggcgatg aagccgcact gacatctgca cttcagggag
    38761 tggaaaaact actgctgatc tcttccagcg aagtgggtca acgtgccccg cagcatcgta
    38821 atgttattaa tgccgcaaag gcggctggcg tgaaatttat cgcttatacc agcctgctac
    38881 atgcagatac ctccccgctc ggcctcgccg atgagcacat tgagacggag aaaatgctgg
    38941 ctgattctgg catcgtttac accctgctgc gcaacggctg gtacagcgaa aactacctcg
    39001 ccagcgcccc ggcagcactg gaacacggcg tatttatcgg tgcggcgggc gatggcaaaa
    39061 tcgcctcagc aacgcgggca gattatgcgg cagctgcggc acgcgtgatt agcgaagccg
    39121 gtcacgaagg caaggtttac gaactggcgg gcgatagtgc ctggacgttg acacagttag
    39181 cggcagagct gaccaaacag agcggcaaac aggttaccta tcaaaatctg agcgaagccg
    39241 atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg actggcggat atgctggcgg
    39301 attctgacgt tggcgcatcg aaaggtggtc tgtttgataa cagcaaaacg cttagcaaat
    39361 tgattggccg cccaacgaca acgttagccg aaagcgtaag ccatcttttt aatgttaata
    39421 actagttaat taaagtggca tcctcccgca tcctctctga taatgacggg atgccgggag
    39481 caatcatgtc tgcttcctga actttcttct gacagaccaa tggatgccag taatgattag
    39541 cggcgtgctg tacgccctgt tagcagggtt gatgtggggg cttatttttg tcgggccgtt
    39601 gatcgtaccg gaatacccgg cgatgttgca gtcgatgggg cgttatctgg cgttagggtt
    39661 aattgcgctg cccattgcct ggctgggacg cgtgcgtctg cgtcagttgg cgcgtcgcga
    39721 ctggcttacc gccttgatgc tcaccatgat gggcaacctt atctattact tctgccttgc
    39781 cagtgccatt caacgtactg gcgcgcctgt ttccacgatg attatcggca ccctgccggt
    39841 ggtgattccc gtttttgcca atctgcttta tagccagcgc gacggcaaac tcgcatgggg
    39901 aaaactcgcc ccggcactgg tttgtattgg catcggcctg gcgtgtgtga atattgctga
    39961 gttaaaccac ggactccccg attttgactg ggcacgttat acctctggca tcgtgctggc
    40021 gttagtttcc gtggtctgct gggcgtggta tgccctgcgc aacgcccgct ggctgcggga
    40081 aaatcccgac aaacatccga tgatgtgggc aacggcgcag gcgctggtca cactgccggt
    40141 ttctctcatc ggctatctcg tcgcctgtta ctggctgaat atacaaacgc cggacttctc
    40201 cttacctttt ggcccccgtc cgctggtgtt tattagtctg atggttgcga tagccgtgct
    40261 ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc cagcgattac cgacagtgat
    40321 tctcgggccg ctgattgttt tcgaaacgct ggcaggtttg ctgtacacct ttttactccg
    40381 ccagcaaatg ccgccgctaa tgacgctgag cggtatcgcg ctgttagtga ttggcgtggt
    40441 cattgcggtc agagcaaaac cagaaaaacc tttaactgaa tctgtctcag aaagttgaca
    40501 cgctggcagt gagttaaata agcctctgct acgtaagggt tatagctttt gccttaaaga
    40561 tgcatttaaa atacatctta tattattaag aatgaggtat cagctatggc ttatcgcgac
    40621 caacctttag gtgaactggc gctctctatt cctcgcgctt cagctctgtt tcgtaaatat
    40681 gatatggatt actgctgtgg cggtaagcaa acgctggcgc gcgcggcggc acgtaaagaa
    40741 ctggatgttg aggtcattga agctgaactg gcaaagcttg ctgaacaacc gattgagaaa
    40801 gactggcgta gcgccccgct ggcagaaatc attgaccata tcatcgtgcg ctaccacgat
    40861 cgtcaccgcg agcaactgcc ggagctgatc ctgcaagcga ctaaagtcga gcgcgttcac
    40921 gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc tgactatgct gcatgaagag
    40981 ctatccagcc acatgatgaa agaagagcag atcctcttcc cgatgatcaa acaaggcatg
    41041 ggcagccagg caatgggacc aatcagcgtg atggaaagcg agcacgatga agcgggcgaa
    41101 ctgctggaag tgattaaaca caccaccaat aacgtcacac cgccgccgga agcctgcacc
    41161 acctggaaag cgatgtataa cggtattaat gaactgattg atgacctgat ggatcacatc
    41221 agcctggaaa acaatgtact gttcccacgc gcgctggcgg gtgagtaatc taaaagctgg
    41281 atggcattgc gccatccagc atgataatgc ggttatttcc gcagttcagc agcccgcttc
    41341 ttaccaataa acaaccagcc cagccccagt gcgataaacc acagcggggt gaccaacagc
    41401 gcctggcgag tgtcatcttc cagtgtcagc aacacgacca cgaacacaaa gaacgccata
    41461 catacccagc acatcagctt gccgagcggc atcttgtaga tcgacttctc atgcagatga
    41521 ggacgctgtt tgcggtaaac caggtacgag caaaggataa ttgtccagac gaacataaac
    41581 agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa tcacgctagg attcacatac
    41641 aacatcacca cgccgccgag cagacagata cacgagaagg tcaacccttt cgcgggtact
    41701 gcgcgcttag aaagtttagc gaacgctttc ggtgccacac cttcctgcgc cagaccaaac
    41761 agcatacggc tggtagagaa gacgccgctg ttagcggaag acgccgcaga ggtcagcacc
    41821 acaaagttga tcacgctggc agccgcaggc aaacctacca acacaaacag ttcaacaaac
    41881 gggctcttct ccgggactac cgaactccac ggcgtcacgg acataatcac aatcagcgcg
    41941 aagacgtaga acataatgat acggatcgga atggagttaa tcgcgcgtgg cagtgatttc
    42001 tccggatctt tggtttccgc agcggttgta cctaccagct caatccctac gaaagcgaaa
    42061 accgctatct ggaatccggc aaagaagcca cttaaacctt tcgggaacca gccgccatca
    42121 ttccacaaat gcgcgaatga cgcttccaca ccagtcggtg actgaaagtg catcgccacc
    42181 ataaccaggc cgacgacaat cagcgacaca atggcgacga ttttgatcat cgcaaaccag
    42241 aactccatct caccgaacat tttcacggtg gcgagattga gcgtcagcag cagcactatc
    42301 accgccagcg aggcgaccca gtcggagaga tcggggaacc agaactgagc ataagcagtg
    42361 atcgccacca cgtctgccat accggttaca acccagcaga accagtaagt ccagccggtg
    42421 aaatatcctg cccacggccc gagtaaatcg gaagcgaagt cactaaaaga tttgtattcc
    42481 agattcgaaa gcagcaattc ccccattgcc cgcatcacga aaaagagcat aaaaccgatg
    42541 atcatataaa cgaaaatgat cgacggcccg gcaaggctaa tcgttttgcc ggaccccata
    42601 aacaaccccg taccaatggc accgccaatg gcaataagct gaatatgtcg gtttgtgaga
    42661 ttgcgccgta gcgactgttc agccggagcc tgatcatcgg caacgacttt tacctgatct
    42721 accatgtttt tttcttcctg tacctgtctg tgttgttcag gctctacggc ctttagtcag
    42781 tctatgatac gacgaatctg tatcgggact catcgatatt aggtaacaat acgcgggatg
    42841 aatactaaga tttagatcta atgttaattt tatgtttaaa gtgagtgtca tatcacggtg
    42901 ataacgcgaa acagctcaca aaaatacacg cttacggggt ctttgcaaca caaaatagcg
    42961 ccgtttggca aactgaaggg tttattgctg aatgcctgct cccctctggt tcgaggggaa
    43021 cagaatgcgc tgcttagagg atttccagca gttcgacttc aaacaccagg gtgctgaacg
    43081 gagggatgga tgcgcctgcg ccgcgctcgc catatgccag ttcctgcggg atagtcagtt
    43141 cccatttaga acctaccggc atcagagtca gtgcttcaat ccagccaggg atcacgccat
    43201 taaccgggaa ttcagcgggt tcaccacgag caacggagct gtcaaacacg gtgccgtcga
    43261 tcagtttacc ggtgtaatga acacgaacgc ggtcggtacg tgccggaatt gcgccttcac
    43321 cctggttgat cacgcggaat tgcaggccag attcggtgct attcacacct tcttttttgg
    43381 cgttttcttc caggtatttc acaccttcag cagccatcgc ctggaaacgc tgacgacgaa
    43441 cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg caccacatca accggcacag
    43501 ccggatgttt gccttccagc gcatcggcaa tacctgcaac cagtgcttct ggcagcagcc
    43561 cttccaggcc agattcactc agttgttgcc ctacctgcaa accaatgccg tagcttgctt
    43621 gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt tcctttctta gggtataaaa
    43681 gtagcgggca gcatatcagc cacacttctt tgggtaaaac tttgtcgcag gaatgatgac
    43741 aaatcccggg gaaaaagaaa caatagcaat atcccgtatg aatataataa aggcgggttt
    43801 accacggaca tcatagcgtt agcttattca taggctatga ttgaggaaca agacgcggag
    43861 caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac
    43921 gcgccggaca attttcgctt catggacccg ctgccgccga tgcatcgtcg ggggatcatt
    43981 attgccgcca ttgtattggt ggtcggattt ctgcttccat ctgatgatac gcccaacgcg
    44041 ccggtggtga cgcgtgaagc gcagttagat attcagtcgc aatcacaacc gccgacagaa
    44101 gaacagctgc gggcgcaatt agtcacacca caaaatgatc ctgatcaggt ggctccggtc
    44161 gctccagaac ctatacagga aggccagcca gaagaacagc cgcaaactac ccaaacgcaa
    44221 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    44281 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    44341 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    44401 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    44461 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    44521 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    44581 ggggtgaaac catactgatg tgataaacca ggccaagcgt atgctggttg atcaaataat
    44641 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatatct ggcttaaaaa
    44701 tattgtgttt cttcccttca tccaataagg attccattaa ctgcaagtgc gcatggttaa
    44761 tttctaagag acgcagtgat tttgcataat gcacaccttt actttgattt tcactatgca
    44821 caatttttaa aaaccaaggg t
//
LOCUS       DAJVYM010000013        43714 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVYM010000013 DAJVYM010000000
VERSION     DAJVYM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.013"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..560)
                     /locus_tag="OQS60_000711"
     CDS             complement(<1..560)
                     /locus_tag="OQS60_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS2386557.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(763..999)
                     /gene="feoC"
                     /locus_tag="OQS60_000712"
     CDS             complement(763..999)
                     /gene="feoC"
                     /locus_tag="OQS60_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS2386558.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(999..3320)
                     /gene="feoB"
                     /locus_tag="OQS60_000713"
     CDS             complement(999..3320)
                     /gene="feoB"
                     /locus_tag="OQS60_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS2386559.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="OQS60_000714"
     CDS             complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="OQS60_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS2386560.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4002..6323)
                     /locus_tag="OQS60_000715"
     CDS             complement(4002..6323)
                     /locus_tag="OQS60_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS2386561.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6420..6896)
                     /gene="greB"
                     /locus_tag="OQS60_000716"
     CDS             complement(6420..6896)
                     /gene="greB"
                     /locus_tag="OQS60_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS2386562.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7124..7843
                     /gene="ompR"
                     /locus_tag="OQS60_000717"
     CDS             7124..7843
                     /gene="ompR"
                     /locus_tag="OQS60_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS2386563.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7840..9192
                     /gene="envZ"
                     /locus_tag="OQS60_000718"
     CDS             7840..9192
                     /gene="envZ"
                     /locus_tag="OQS60_000718"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS2386564.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="OQS60_000719"
     CDS             complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="OQS60_000719"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS2386565.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11269..11583
                     /locus_tag="OQS60_000720"
     CDS             11269..11583
                     /locus_tag="OQS60_000720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS2386566.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11596..12969
                     /locus_tag="OQS60_000721"
     CDS             11596..12969
                     /locus_tag="OQS60_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS2386567.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="OQS60_000722"
     CDS             complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="OQS60_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS2386568.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="OQS60_000723"
     CDS             complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="OQS60_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS2386569.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="OQS60_000724"
     CDS             complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="OQS60_000724"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS2386570.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="OQS60_000725"
     CDS             complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="OQS60_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS2386571.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17607..18167
                     /gene="nudE"
                     /locus_tag="OQS60_000726"
     CDS             17607..18167
                     /gene="nudE"
                     /locus_tag="OQS60_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS2386572.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="OQS60_000727"
     CDS             complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="OQS60_000727"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS2386573.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21004..21783
                     /gene="hofM"
                     /locus_tag="OQS60_000728"
     CDS             21004..21783
                     /gene="hofM"
                     /locus_tag="OQS60_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS2386574.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21783..22322
                     /gene="hofN"
                     /locus_tag="OQS60_000729"
                     /pseudo
     CDS             21783..22322
                     /gene="hofN"
                     /locus_tag="OQS60_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22306..22746
                     /gene="hofO"
                     /locus_tag="OQS60_000730"
     CDS             22306..22746
                     /gene="hofO"
                     /locus_tag="OQS60_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS2386575.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22736..23140
                     /gene="hofP"
                     /locus_tag="OQS60_000731"
     CDS             22736..23140
                     /gene="hofP"
                     /locus_tag="OQS60_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS2386576.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23052..24290
                     /gene="hofQ"
                     /locus_tag="OQS60_000732"
     CDS             23052..24290
                     /gene="hofQ"
                     /locus_tag="OQS60_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS2386577.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24691..25212
                     /gene="aroK"
                     /locus_tag="OQS60_000733"
     CDS             24691..25212
                     /gene="aroK"
                     /locus_tag="OQS60_000733"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS2386578.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25269..26357
                     /gene="aroB"
                     /locus_tag="OQS60_000734"
     CDS             25269..26357
                     /gene="aroB"
                     /locus_tag="OQS60_000734"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS2386579.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26449..27735
                     /gene="damX"
                     /locus_tag="OQS60_000735"
     CDS             26449..27735
                     /gene="damX"
                     /locus_tag="OQS60_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS2386580.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27842..28678
                     /gene="dam"
                     /locus_tag="OQS60_000736"
     CDS             27842..28678
                     /gene="dam"
                     /locus_tag="OQS60_000736"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS2386581.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28696..29373
                     /gene="rpe"
                     /locus_tag="OQS60_000737"
     CDS             28696..29373
                     /gene="rpe"
                     /locus_tag="OQS60_000737"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS2386582.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29366..30124
                     /gene="gph"
                     /locus_tag="OQS60_000738"
     CDS             29366..30124
                     /gene="gph"
                     /locus_tag="OQS60_000738"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS2386583.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30117..31121
                     /gene="trpS"
                     /locus_tag="OQS60_000739"
     CDS             30117..31121
                     /gene="trpS"
                     /locus_tag="OQS60_000739"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS2386584.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31281..32186
                     /locus_tag="OQS60_000740"
     CDS             31281..32186
                     /locus_tag="OQS60_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386585.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32203..32565
                     /locus_tag="OQS60_000741"
     CDS             32203..32565
                     /locus_tag="OQS60_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS2386586.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32649..33812
                     /locus_tag="OQS60_000742"
                     /pseudo
     CDS             32649..33812
                     /locus_tag="OQS60_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33812..35038
                     /locus_tag="OQS60_000743"
                     /pseudo
     CDS             33812..35038
                     /locus_tag="OQS60_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35035..35913
                     /locus_tag="OQS60_000744"
     CDS             35035..35913
                     /locus_tag="OQS60_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS2386587.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35924..36277
                     /locus_tag="OQS60_000745"
     CDS             35924..36277
                     /locus_tag="OQS60_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS2386588.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36289..37593
                     /locus_tag="OQS60_000746"
     CDS             36289..37593
                     /locus_tag="OQS60_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS2386589.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37605..38690
                     /locus_tag="OQS60_000747"
     CDS             37605..38690
                     /locus_tag="OQS60_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS2386590.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38842..39573)
                     /gene="phoB"
                     /locus_tag="OQS60_000748"
     CDS             complement(38842..39573)
                     /gene="phoB"
                     /locus_tag="OQS60_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS2386591.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="OQS60_000749"
     CDS             complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="OQS60_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS2386592.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="OQS60_000750"
                     /pseudo
     CDS             complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="OQS60_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="OQS60_000751"
     CDS             complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="OQS60_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS2386593.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42379..>43714)
                     /gene="frlA"
                     /locus_tag="OQS60_000752"
     CDS             complement(42379..>43714)
                     /gene="frlA"
                     /locus_tag="OQS60_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS2386594.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11128 c        11988 g        10441 t
ORIGIN      
        1 tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg gtgctgcatg
       61 atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc ataaagctgc
      121 cttgccagtt tgggatcggc gaaacaatcc agccaacaca gcgaataggg ataggggctt
      181 tcaataccgt ggtaaaacaa caatagcacc accatcggca acgttttgta tccagcatca
      241 aggtgatttt gcattgcggc aatagcgtaa cgcatcatgc gaaatgcgat cagtttgttt
      301 gaggtgcttt gatgttcaat cagacaatag atgtatcctg gtccttgttc cgttttcacc
      361 gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct gctcgactcc
      421 agcttgaggg ttttcatatc acaaagcgcg tgaatgggtg ccggtaaatg aaacgcaaga
      481 aaatcacgag ccgtgtccgg ttggcgtaaa aagagtttga acagcgcatc gtgtggggtg
      541 gaactctgct ttttgctcat ggcgttcctt cacctgttgt ttgaatgccg cgactctacg
      601 cgccactcca cacctgtgca ttagcgagtt ttcatccctg cgagatgctt tcaaaggaac
      661 ttacgccgtg ctgcaagagt tgcattttta tggaaagccg cacgcaaaaa agcccggatg
      721 cagacgcata ccgggctttc caaaacaggc gatggagtaa ggttaacgca gcgcccacca
      781 ctcgcgcaga caggcttttc cttccgggca gcttttacaa ctgccagaga ggcagccgtc
      841 aggttcttcc tgaatccgca cggctttgcc catactttcc agttgttgca gcatggcgtt
      901 aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca tacggccccg
      961 taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc accggtggtg
     1021 ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat atccacccgg
     1081 ctacgcgcgc ggcgcagcag accgataact acgatgttaa acaggattac cgccagaatg
     1141 cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa caatgttgcc
     1201 agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc acggcttgat
     1261 tcacgggcga tggctcccat caccgagata catggtacat acagcaggac gaaaatcagg
     1321 tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat cgccccggta
     1381 cccatttcac cgtcgccttt gctagcttca atggggttca tcagtacgct aaggctgaag
     1441 gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc accgaggttg
     1501 aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag ggtgttgagc
     1561 gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt tgcctgccag
     1621 ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct gacggacgcc
     1681 agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct gttgaaagcg
     1741 ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa gcccttcaga
     1801 cgctgccagg tctggataat caggctttta acgtgtggta catggtagac cggcagctcc
     1861 atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag gccagtcagc
     1921 accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc gttctgcccg
     1981 aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga cataaacggt
     2041 gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc gcccattacc
     2101 gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc cggcaagccc
     2161 agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc ggagtcctca
     2221 aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag cacggtgtta
     2281 ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa gtggagcgtg
     2341 tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtc aaacagcggc
     2401 tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac aaagaggaaa
     2461 atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt gaaacggctg
     2521 ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagctatgca ctggtaacgc
     2581 gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc aagggcggca
     2641 tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc ttccagcatt
     2701 tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac ttttgccagt
     2761 gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt ctcgttagct
     2821 ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt tgaaaccagc
     2881 gggatcactg ggcagccaag acgcgccgac agagcatcaa tttcaatacg aatattttgc
     2941 ttctcggcaa tgtcgagcat gttcagtgcc acgatgcagg gaatgccgag ttccagcagt
     3001 tgtagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt aatcagcagg
     3061 tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga ggtctgcgat
     3121 gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg atgatcggtg
     3181 gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc tacacgctga
     3241 cgtgcgctag tgagctggtt aaataaggtt gtcttgccag aatttggatt accaattaag
     3301 ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg cttccacttc
     3361 taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt cgatatgaat
     3421 ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta acatgccaag
     3481 agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag tgattttcca
     3541 cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact ggataactac
     3601 ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacaaag gagagattaa
     3661 ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta aaatgtgtgc
     3721 gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt tatatcgcag
     3781 cgaaaatata attcgaatag tgaattaccc ggactggcta atgtttaatt aaggtttcag
     3841 ttgttaatga agtgataaat attgccggaa tatattatga gtttggttgt ggaataaaaa
     3901 ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc agatgcggcg
     3961 tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt tttgcccatt
     4021 gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc acgaccgcgt
     4081 ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg acgggcgttg
     4141 gtttcgccag gctgctcgtc cagacgcatg gtcagggcga tacgtttacg ctgaagatcc
     4201 acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg cggatcttcc
     4261 acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac gccaatatcg
     4321 acaaacgcgc caaagttggt gacgttggtg acagcgcctt cgaggatcat acccggttgc
     4381 aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc cggacgcgga
     4441 tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac accgaatttt
     4501 tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc catcagatct
     4561 ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc ttccgggtga
     4621 acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc cgcgcactgc
     4681 tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt cttgaactgg
     4741 ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag gcccgccacg
     4801 cgagttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt tacgcagtct
     4861 tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg ctgatactga
     4921 cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc ctgcaaacgg
     4981 cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc ctgcgctgcc
     5041 agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt ctgtgcggtc
     5101 actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg ggaagctgta
     5161 ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc aacggtcatc
     5221 gctacttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac cagtttgcca
     5281 gtggcatcga ccactgccac ttttaccccg gtacgcagac ccggatcgag gcccatcgtt
     5341 gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc aaagacgttg
     5401 attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc cagatgcatc
     5461 agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc cgccggggca
     5521 ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct ctctttgggc
     5581 ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc gttacgccca
     5641 cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg atgatcgaaa
     5701 tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga aaccaaatgc
     5761 gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc ggcaaaccgt
     5821 tccatcagga tatagcgcgc gccgtccagc gcggctttgg tatctgccac gcctttatcg
     5881 gcatcaatat attgcgcagc ggcgacttct ggtgtgtgtg acggatcgct ccacagcagg
     5941 tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg gcgtttaggt
     6001 ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt gatggccttc
     6061 gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc ctgacgtctc
     6121 tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg cgtgtcatcc
     6181 agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt cccttcgtca
     6241 agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc cgcaataatg
     6301 cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa aatcagggga
     6361 tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa gactcttcat
     6421 tacggtttca cgtattcgat agcattaaca taccagctcg cttccccggc aggggtattc
     6481 accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg ggaatcgata
     6541 gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa acggtgagtc
     6601 acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc ttcctgctga
     6661 ggggagtaat tgacgatttt gagattttcc atgcatttag tgagatagcg cacgcgacgg
     6721 tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct gcggtcgccc
     6781 agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg ccagagataa
     6841 ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt tttcatctcg
     6901 ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc atatcaacag
     6961 aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat atgctttgta
     7021 acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc ttttttaaga
     7081 atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag agaactacaa
     7141 gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt atctcaccga
     7201 acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc tgctgactcg
     7261 tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg gcttgtcgat
     7321 ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga cggcgaaagg
     7381 ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca ttccaaaacc
     7441 gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc aggcgaacga
     7501 actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt tcaaacttaa
     7561 cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca gcggtgagtt
     7621 tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg ataagctgat
     7681 gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg tgcagatttc
     7741 gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc agaccgtttg
     7801 gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt gcgcttctcg
     7861 ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgccagcctg
     7921 gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat
     7981 aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc
     8041 acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct
     8101 ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca ctatgaattc
     8161 ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac
     8221 aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtacgcgtg
     8281 ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac gctggcgatt
     8341 atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg accgttggtc
     8401 gatctcgaac atgctgcttt gcaggtcggt aaagggatta ttccgccgcc gctgcgtgag
     8461 tatggcgcgt ccgaggtgcg ttccgttacc cgcgccttta accatatggc ggctggtgtt
     8521 aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg
     8581 ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca
     8641 gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac
     8701 ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt gctcggtgag
     8761 gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc
     8821 attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac
     8881 gccgctcgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc
     8941 tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc
     9001 cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca
     9061 attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg
     9121 ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca
     9181 aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct ataagatact
     9241 ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta ccagcgcggc
     9301 acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg ccagggtttc
     9361 ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga gaatcttcgt
     9421 gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag tgaaggtttc
     9481 tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat ctttaatcga
     9541 gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg cgcccgccgc
     9601 ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca ggaacgccgc
     9661 gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag taccggccag
     9721 tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca ggcgagaaac
     9781 cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg cgtggcccgc
     9841 tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa cgcgggtgtt
     9901 ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg tgacgttttc
     9961 cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt tcgacagctt
    10021 gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt cccagccgtg
    10081 ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt taccggtgcc
    10141 ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg agcagtgcat
    10201 agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc ctttcttcat
    10261 ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt taaacgccac
    10321 gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact tcgcgccgtt
    10381 cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc gaataaacat
    10441 gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg aaagacgagt
    10501 atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg aaagctgcct
    10561 ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt tgtcgttctt
    10621 acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac ggacgatata
    10681 cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg cacccagatt
    10741 agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct gatacagcag
    10801 gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag cctcgagttc
    10861 ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat atgtattgcc
    10921 tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat ttacgtatct
    10981 ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg ttgctattat
    11041 cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct tggtttatat
    11101 ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa ttcaaccggt
    11161 aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag caaaatacta
    11221 ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat ggataacgtt
    11281 gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca ggggctggtt
    11341 tgctacctgc ttatcgcctg gcttgccgga aaaaatcaca gttggattgt ttatggcgtg
    11401 cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt taaacagaag
    11461 cgcctttggg gatggctggc gctggtgttt attgccacga cagggatgag cggttggctg
    11521 aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgatttg ggattttggc
    11581 tgatatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag ccttcgtatc
    11641 cgcaatggca gtagccgcta tagctacttt taccagtcag tatggcataa cgtacttata
    11701 ttactggtga tttttctctc caatggcctg acgtggctgg tgcttttact gtggggtgag
    11761 ttgtttaaac ttgttggcat cacgtttttc aatacgctct tttttgcaac cgactggttt
    11821 atgtatctta catttggtct ggttaccgca ctggtggtga tcctcgcgcg tacacagtca
    11881 cgtttaatcg actctattca aaagttgttc acgctaatcg ccacggggtt gctgccgtta
    11941 gtatcattgc taaccctgat gtttatcatc accctgccgt ttacggacct gagcgcgatt
    12001 tctcgccaca tctccgccgc cgggttgctg ttaacgctgg cctttttgca attgatctta
    12061 atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc gttgcgttgc
    12121 ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc ctgggcgtta
    12181 tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgc gctggcggtg
    12241 ctggtgttac tggtgtggtc gctggggtat tttgtcagta tcgtctggcg taatgggcaa
    12301 aatccccttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt gttggtgata
    12361 ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa cagtcatatg
    12421 gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat gctcgaacag
    12481 agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccgg gtttatgaaa
    12541 gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca tcttcaggaa
    12601 caggtatcgg aaaaagtatt agccgaaaat gtgttaattg ctcctggttc tgttaaaccg
    12661 gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac ctgtattgaa
    12721 aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc ggagcggatc
    12781 ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga cagaaaagaa
    12841 tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa attactcaca
    12901 gctgcgaagg atggaaaact ggatctgacg gtagatggtg aaacgctgga aataaactta
    12961 agtaagtgac aactctttta acggatacga cgctgtatct gaccaacaat aatttgcgat
    13021 ttatttcaca ggcctggtaa gcgcagcaca tcaggccatt acgtcgtaaa atccggcaga
    13081 gccctctctg ccggacatac tcattaatga acttgcggat ctgccggaga cgcgttgttg
    13141 cggatttcag caatatccat cgcattgaac agatagtggt taccgcagta atcacaatgc
    13201 atgtcaattt cgccatcttc cgccaggatg ctatcgactt cttcatcagg cagcgttttc
    13261 agcgcgtcgg cgcaacgttc acgcgagcag gtgcatttga actccacatc ctgcggatcg
    13321 taaaccgtca cctcttcttc gtgatacaaa cgccacaaca cttcgtttgc cggtaaggtc
    13381 agcagttctt cggttttgat ggtttcggtt agcgtcgcca ggtggtcaaa gtcgtcctgc
    13441 tgggcatttt gcgcaggcat tacctgcaac aacataccgc ctgcagccgg tttgccgtct
    13501 acgtcgccgg tgcgaataaa caggcgcgtc ggcagctgtt cagaacgcat aaagtaatct
    13561 tccaggcagg ccgccagggt atcaccttcc agaccaacca cgccctgata gcgttcgcct
    13621 tcgctcgggg taatggtgat caccacgtaa ccattgccga ccagcgtttt caggtcggca
    13681 ttttctggaa tttcgccctg cacgcgcgcc acaccgcgca tttgctggtt attgttaccg
    13741 ttaataaccg ccagattcat cggaccgtcg ccttgcagct gtacggtgat atcaccatca
    13801 aacttcagcg tagcggttaa caggctggtc gcaaccagca gttctgccag cacgttttta
    13861 acgggctgcg gataatcgtg attctcaagg atctgttgca gggtttccga aacggttacc
    13921 agttcgccgc gcacggcaaa gttttcaaac agatagcgat gtaattggtc atgttgcggc
    13981 ataatcatct ctcttgcagg tgacagttat tcactgtcgc cgtgtttaaa tcgtaacagg
    14041 tcgcggcgct cttttttgtc cgggcgtcgg tccgggtgcg gcatggttaa ggcattaagt
    14101 ttacgtgcca gcgccatttt ttcgcgtttc tctacacttt ccgcagtctc ttcatacagc
    14161 aaggctgcct cgctggcggg gcgacgctgt tcagtaatcg cctttacaat caccgtgcgt
    14221 tcgtcatttc cctggcgcag agtgagcgtg gcattcagct cgacgatttt gctcggcttg
    14281 ctgcgctgcc cgttgtaatg caccttaccg ccttcaatca tttcacgggc cagcgcgcgg
    14341 gttttataaa aacgggcagc ccatagccat ttatccagtc gaacctcaac agcaggtttt
    14401 tctttcatgg cgtctccttc acattagcga ggggatcagg cggcggtagt cattcagtga
    14461 cggatggcgt tgatactgtt tctcggcaat cccggaatca ggattagtca cgccgaggca
    14521 gtaacgaata ccaaattgcg cggcagcatc gagaatcgct tcgctgtcat caataaacag
    14581 cgttctttca gctttcagac ccgtagcttc ggccaccgca tgccataacc gctgatcctc
    14641 tttcggataa ccaaatgtgt gggtggaaag taataaatca aggtgtgcgt ccagaccggt
    14701 atgctcaagt tttaccgcca ggttgtgcgg atgcgcattg gtgagcaaaa ttcgctgctt
    14761 accgctggct ttcagtgcct caagaaacgg aatggtatct tcacgcagta cggcacgcgg
    14821 tcccatctcg gtggtcatcg cacagatatc cagacccagt tgctcactcc agtaatcaag
    14881 acagtaccag tttagcgtat gctgtacgtc gtgatattgc tggcgcatat attccatcgc
    14941 ttcctgtggc gtaaccccgt ttttcgcgcc ccatgtttca ggcaccagct tttgccagaa
    15001 atagttatcg aaggcgaggt cgagcaacgt gccgtccata tccagcagaa cggtatctac
    15061 gtcctgccag gcaatgttga tatgcatgag ggaaatctcc agagtgaagc aatttgcgcg
    15121 acagggtagc ataacctgcc gcgcaaacgt gttattcgat aaggctttct gaaggggtga
    15181 tcagttgcgg gttcaggcag ctttcataat aggcctgaat cttcatcatg cgagtgcgat
    15241 gacgctggta acgacgccag gcctgtacgc cattgtaaat ggcgctaccc agcatcgtca
    15301 gtagcagcaa tgtggtgctg agatagcgcc acaggccgga acgatccggg attggatgaa
    15361 ggccaatatg ctgcgtacca ttggcgtcag tgaagatttt tgtgacgata ccctcggcgt
    15421 taaacggcgt atgcatcagc atttgtgcca gtttctggaa agcgttccac tgttcttgcg
    15481 gcgggtagtc gtaaagtgat gccgaaggcc agggctgatc aacaaaatcg ctgccttcgt
    15541 cgctgacaat caggaatccg ccgggggccg gactgtttag tgattgtgcc gctcgcgccg
    15601 tttcatgcgt gataaacggc gcggtggagg ttgccaccag gttatccagc gattccgcac
    15661 tcaccgggcg taataacaca ttcacgccat cgagcttccc ggcgttggcg cgttttacca
    15721 gcgcgtccca gtctttactg ttgccgagat tgaccagcgc atttttcagt cgcacacagt
    15781 catctttggc agaacataaa tccgctgtct tcagtacaat gtcgccaaaa tcatcaagca
    15841 ataccatgcc ggatttttga attgctgaac gtaatgaggc actgacgcga gatccatctt
    15901 ccggtttagg gtgcagctgg cgattaactg cttcagtcaa tgccgtcgct ttgttgacca
    15961 gttcagattc tggtaatggc aatgagcggg cgtcgttcca gatgatctgc gagcagtcaa
    16021 acggtaaaaa aggtgaattg gttttcgcgc tccaggttcc ggaagtgcga atattacaca
    16081 ttcccgtacc gctgatacgc aatgtatcgc ctacccgcac gccagcgtca gccagttgtt
    16141 ttacgctggt ggcttcaatg gtctgtgcgc ctttcatcca tgagagagtg aatttcagcg
    16201 gcatatccag cgggatccag aataacagca taaacagcac cagcagcgag cccgcagcga
    16261 taatcgtact gcgcagccag tgctgtaacg gaaagttttt tacttcatca tgcagcgaaa
    16321 gatatcgccc ctgacgcact acgtggcggt cgagatagat atcaatatcg gtttgttgac
    16381 cgagatcctg agcaatgtat ggctgccagt gtgcgggata gaccaggtcg ataataccga
    16441 gtgaaatatt gttgatctgt tcctgatcgt tttcgccaaa caatccccaa cgacggggtg
    16501 taccgcgcag acaatgaatt tcccgcaggg aggattttgc cgggggcgcg aatagccccc
    16561 acagacctgc gcccagcagc agtaatgcgc cgcctgccag ccacggaaca aatacatccg
    16621 gggtaatcag gcagaaaaag aacatcagga aggaggcgac gatcagcaac gcttcacgca
    16681 gcccgcgcgg acgactcagc gcatattctt catgcgtttc tttgcgaata ttgagtagct
    16741 cgatttgctc actctcttcc ccgcgaatcg acgcctgcgt ggacggaaca ggttgcaagg
    16801 catagccgcg cgtttcctgc atgtactcct gcagcgtatg accgttgagt gaaataacca
    16861 gcggcagcga atcggtataa atcagttcaa cggtattctc atcgttgatg tattgttccc
    16921 agaaaggggg caggtggact tctaccgaat cgaggtagta acgccattta ttcggatcgt
    16981 cggtagagat gccgtaacgc gtgatagcgt gtgtcagcat catgacgttg ttgctttcgg
    17041 cattcagcgc cagagagatc ggtgccgcgc tagctcccgt tgggccaggc acctgtaata
    17101 cctgcgtcag gctctcaaga taattttcaa cggcgctacg ttcttcaggt gtgagtttac
    17161 gcgtttgcgc atccgcgaag gcgttggtcc agggcagctg acgccgtctg gatcgcactt
    17221 ttatcagcca tcccgcaagt agtgagcagg ccagcaaagc agctaaaaaa atcacaatgg
    17281 tgctcatgct ttccccatct tacttagtct gtcaggcgtg gtcagtcgca ccagacacaa
    17341 tacatgattc tgcggttggc tatcggcaag cgtggagttt aacttgagca tcattaagca
    17401 catcacagta accgctgatc atagcaaacc ctctgaaggc gggatatcag caaattcctg
    17461 gagttatttc aacctattga cttttaattt gatttgagga cgattattta ttaagttaca
    17521 taaatgtaaa taacatgcaa ttcacattgc cgaaagcgtg tccgatatcg cacaataacc
    17581 gctgatttca cagcatagat cccacgatga gcaaatcatt acaaaaaccc accattctga
    17641 atgttgaaac tgtagcccat tcccgactgt ttaccgtcga gagcgtggat ctggagttca
    17701 gcaatggcgt gcggcgtgtt tatgaacgaa tgcgtccaac caaccgggaa gcagtgatga
    17761 ttgtgccgat tgtggacgat cacctgatcc tgatccgcga atacgcagtg ggaactgaat
    17821 cctacgaatt aggtttttcg aaaggattaa ttgatccggg tgaaagcgtc tacgaagccg
    17881 ctaaccgcga gctaaaagaa gaggttggat ttggtgcgaa cgatctgact tttttgaaga
    17941 agctcagcat ggcaccgtct tacttttcca gcaaaatgaa tatcgtggtc gcgcaagatc
    18001 tctacccgga atcgctggaa ggcgatgagc cagagccgct accacaggtg cgctggccgc
    18061 tggcgcatat gatggatttg ctggaagacc ctgacttcaa cgaagcgcgt aatgtcagcg
    18121 cgctgttcct cgtgcgcgaa tggttgaaag ggcaggggcg agtgtaaatg cgtgaagttt
    18181 gcctgatgca caacgattat caggcttatg gggttcctgc aatatattga tttttcacga
    18241 ttttgttagc cggataacgc gttcacccgc atccggcata aacaaatgca ctttgtcagc
    18301 caactgaaaa ggcgccgaag cgccttttta atcagaacaa ttcctgtgcc tcaccattat
    18361 cgataatggt tgttcccacc tcgtgcaccg cctgttgtgt cggctgcgta ccttcgatga
    18421 aatactcttc gcggctgttg ccaccattag ctaactgccc ggtgctgcga tcgatattca
    18481 ccgtcacaat acccggaggc ggcgtcagcg gctgctccgg cacaccttca agaacggctt
    18541 tcatataagc gtcccatgca ggctgggcac tcttggcacc gccttcgtaa cctgagatct
    18601 gatctttaat cgctccggaa gccgttgtat gaccgagatt acgacggtga tcatcaaagc
    18661 caatccagac cgaggtcaca acgcccggac cgtaacccga gaaccacgca tctttcgaac
    18721 tgttagtggt accggttttc ccgccgatat cgcgacgctg caaatcacga cctgcacgcc
    18781 agccggtacc ctgccagcct ggttcaccaa agatattggt gttcaaagca ctcttaatca
    18841 ggaatgccag cggagtgttg atgacgtgcg gtgcgtactc ctgcgcgcca gtcttcgcca
    18901 ctaacgcctg atttgcctgc tccagctgcg gcattggtac agaaacattc tgctgctcgc
    18961 gggagatagc gacatcttca acatcgttat tttccagcac gttcgatttc tgcgtatcac
    19021 cgtaaatcac cggaatatcg cattccgggc aggctacttt cggtttcgct tcgaaaatca
    19081 cgccgccctg atcgttttca attttgctga taaaccacgg gtccaccagg aagccgccgt
    19141 tcgccatgac cgcgtagccg cgcgccacct gcattggggt gaaggacgct gaacccagcg
    19201 ccagcgattc ggtgtggaca atgttttgcg ccgggaagcc gaagcgttgc agatattctg
    19261 cagcgtagtc gacgcccatc gcccgcattg cacgtaccat caccacgttt ttcgactgac
    19321 ccagcccctg acgtaagcga attggaccag catactgcgg tggtgagttc ttcggctgcc
    19381 agtcagaacc gacacctgca tcccagcgag aaattggcac atcgttcaac atacttgcca
    19441 gcgtcagacc tttatccatc gccgcggtgt agaggaacgg tttgatgttg gaacccacct
    19501 gacgcagtgc ctgggtggcg cggttaaact tgctctgatt gaaatcaaag ccaccgacca
    19561 gcgccataac ggcaccattt tgcggattga tcgacaccag cgccgagttc acttccggca
    19621 cttgtgccag ccaccatgca tcgccaacct gacgaaccca gatttgctga cccgtttgca
    19681 gaacatcggt cactttacgc ggcgtcggtc cctgctgagt atccgaacgg taaggacgcg
    19741 cccagcgaac gccgtccata ctcaatgcga cagtcgaccc gtccgccagc attgccgtcg
    19801 cttcctgagg attggcgctg gtgactgcgg caggcagcag cggaccatag gtcggcagcg
    19861 ccttcagcgt atcggtaatc ttgttgttat cccacgccga ctcgcccact ttccacagca
    19921 catttgccgg gccgcgatag ccgtggcgca tgtcgtagtc cagcacgtta ttacgtaccg
    19981 cctgctgtgc ggcctgctgc actttgcggg tgatggtggt gtaaatgcga taaccgtctt
    20041 cataggcact ttcgccataa cggttataca tctcctggcg caccatttcg ctcaggtacg
    20101 gcgcagagaa agcaatctcc ggcgcgtgat agttagcgtt aatcgcctca gtgcgtgtct
    20161 gatcgaactg ttgttgggtg atataccctt catccagcat ccgcgacagt acgacgttac
    20221 gccgcgcgac ggcacgatcc attgagtaga gcggattaaa ggtggaaggc gctttcggca
    20281 gcccggctat cacagccatt tcgttcagcg tcagttggtc gaccgtttta ccgaaataga
    20341 cttgtgccgc agcaccgaca ccataggcgc ggtaaccgag gtaaatcttg ttcagataaa
    20401 gctcgaggat ctcgtctttc gtcagcaact gttcaatgcg aatcgcgagg aagacttcct
    20461 taatcttacg catcagcgtg cgttctggac tgaggaagaa gtttctcgcc agctgctggg
    20521 taatggtact cgccccttgt gacgcgtggc cggagaacag cgccacactt gccgcacgga
    20581 agatccccac cgggtcaacg ccgtgatgct cgtagaagcg gctgtcttcc gtcgcgataa
    20641 aggctttcac catctccggc gggatttgat ccaacgtaac cggaatacga cgtttctcac
    20701 cgtattgagc aatcagctcg ccatcggcgc tgtaaatctg catcggaatt tgcaggcgaa
    20761 catcttttaa tgtcgccaca tccggcagtt gtggctcgat gtagcggtat aggccataaa
    20821 tcgagcctgc tcccagcaga atgcaacaga ctgcaaggat caaaaaatac tttacgaact
    20881 tcactggaaa tttcccattt agtttcattt gggcagttta taaacaaacg tgcggtagta
    20941 taaaggcaag ccagacgcat tgatataccc gtcagagtga cgggtgataa ggagatcatc
    21001 acaatggcat ttaagatctg gcaaattggt ttgcatttac aacagcaaga agcagtagcg
    21061 gttgcgatcg tgcggggcgc aaaagaatgc tttttgcaac gctggtggcg gttgccgctg
    21121 gagaacgaca ttatcaaaga tgggcggatt gttgatgcgc agcggctggc taaaacgttg
    21181 ttaccgtgga gtcgcgaact gccgcagcgt catcacatta tgttggcgtt tcccgccagt
    21241 cgcacattac agcggtcatt tccgcgcccg tcgatgtccc tcggtgagcg ggagcaaacg
    21301 gcctggctgt cagggacgat ggcccgcgag ctggatatgg atccggactc cctgcgtttc
    21361 gattacagcg aagactcact cagtcccgcc tataacgtga ctgccgcgca aagcaaagag
    21421 ctggcaacgc tactcactct ggcagaaagg ttgcgtgttc atgtgagcgc gatcaccccg
    21481 gatgccagtg cattacagcg attcctgcct tttttacctt ctcatcagca atgtctggcc
    21541 tggcgtgata acgaacagtg gctgtgggcg acacgctgtc gctgggggcg caaactggcg
    21601 gtggggatga ctagcgcgaa ggagctggcg gcagcgttat ccgttgatcc cgaaagcgtc
    21661 gcgatatgtg gcgaaggcgg atttgatccc tgggaggccg tttctgttcg tcagccgccg
    21721 ctaccgccgc caggtggaga ctttgccatc gcgctggggc tggcgcttgg gaaggcgtac
    21781 tgatgaaccc gccaattaat tttttgccct ggcgacagca acgccggacc gcttttcttc
    21841 gtttctggtt gctgatgttc gttgcgcctc tgctgctggc cgtcgggata acgctaatac
    21901 tgcgtctgac agacaacgcc gaagcacgcg taaacaccgt tttgcttcag gcggaacaac
    21961 aactcgcccg cagcttacag ataacgaaac cacgtttgct ggagcgccaa caaattcgtg
    22021 agcagcgttt gcaacggcag cgccagcgac aatttacccg cgactggcaa tctgcgctgg
    22081 aagcactggc ggcgctttta cccgagcacg cctagctgac aacgataagc tggcagcagg
    22141 gaacgctgga gatcaagggg ctgacaacaa gcattaccgc gttaaacgca ctagaaacgt
    22201 cactccgcca ggatgcgtct tttcatctca atcagcgggg agccacgcag caggatgcgc
    22261 agggacgctg gcaatttgag tatcagttaa caaggaaggt tagcgatgaa catgttcttt
    22321 gactggtggt tcgccacatc accccgcctc cgccagtttt gctgggcatt ctggttgctg
    22381 atgttagtta cgctcatttt tctgtcatcg acacaccatg aagagcgcga cgcattaatt
    22441 cgactacggg caagtcatca ccagcagtgg gccacactgt atcgcctggt tgacaccact
    22501 cccttcagcg aagaaaaaac gctgcccttt tcgccactgg attttcagtt gcccggcgcg
    22561 caactggttt cctggcatcc atccgcgcag ggaggcgagt tggcgttgaa aacgctgtgg
    22621 gaagcagtgc cgtcggcatt tacacggctg gcagagcgca acgtcagcgt gagccgtttt
    22681 tcattaagcg tggaaggtga tgatcttttg ttcacgctac aactggagac gccgcatgag
    22741 ggttaaacgc tggttgttgg caggtattgt attgtgcctt ttaaccggta tgcgtgaccc
    22801 ttttaaaccg ccggaagatc tatgccggat tagcgaactt agccagtggc gctatcaggg
    22861 gatggtaggg cgaggcgagc gcatcatcgg tgtaataaaa gacgggcaaa agaaatggcg
    22921 acgggtgcag caaaacgatg tgctggaaaa cggctggaca attttacagc tgacgccaga
    22981 cgtactaacg ctgggtaccg ggacaaactg cgaaccgcca caatggttgt ggcaacggca
    23041 aggagataca aatgaagcaa tggatagccg cactactgtt gatgctgata cccggcgtac
    23101 aggcggcaaa gccgcaaaaa gtgacgctga tggtggatga cgttccggta gctcaggtgt
    23161 tgcaggcgct ggctgaacag gagaagttga acctggtcgt gtcgccagac gtcagcggta
    23221 cggtgtcgtt acatctaaca gatgttccct ggaagcaggc actacaaact gtagtgaaaa
    23281 gcgccggact gataacgcgg caggaaggca acattctctc agtgcattcc attgcctggc
    23341 agaataacaa tatcgcccgc caggaggcgg agcaggcgcg ggcgcaggca aatctgccgc
    23401 tggaaaatcg cagtataacc ctgcaatacg ccgacgcggg agaactggcg aaagcggggg
    23461 agaagctact gagtgccaaa gggagtatga ccgtcgataa acgcaccaat cgccttttgc
    23521 tacgagataa caaaacggcg ttaagcgcgc ttgaacagtg ggtagcgcaa atggatctgc
    23581 cggtcgggca ggttgagctg tcggcgcata ttgtcaccat taatgaaaaa agtttgcgtg
    23641 agttaggcgt gaaatggacg ctggccgatg cgcaacacgc tggtggcgtt gggcaagtca
    23701 ccacgcttgg tagcgacctc tccgtagcga cggcgacaac gcatgtcggt tttaacattg
    23761 ggcgcatcaa cggacgcttg ctggatcttg agctttccgc gctcgaacaa aaacagcagc
    23821 tggatattat cgccagtccg cgtctgctgg cctcacatct tcagcctgcc agcattaaac
    23881 aggggagcga aattccatat caggtttcca gcggggaaag tggcgcgacg tcggtggaat
    23941 ttaaagaggc cgtcctgggg atggaagtta cgcccacagt gttacaaaaa ggtcgcatcc
    24001 ggctgaaatt acacatcagc cagaacgttc cggggcaggt gctacagcag gccgatggcg
    24061 aagtgctggc gattgataag caggagatcg aaacgcaggt cgaggtcaaa agcggagaaa
    24121 cgttggcgct gggcggcatt tttacccgta aaaataaatc gggtcaggat agcgtaccgt
    24181 tgcttggcga cattccctgg ttcgggcaat tatttcgtca tgacggaaaa gaagatgaac
    24241 gacgcgagtt agtggtgttt atcacgccac gactggtttc cagtgagtaa acagccgtaa
    24301 aagcggtaat gtttttacgc tgaacgtgtt tcatctattt gacgcgcgca ggtatttagc
    24361 atacaaggag taccgatttg agagttggtg ctcttcgctg cctgcgttcc atgatgatga
    24421 tttatcattc aggcggcatt ttgctgtctt ttttacgcta atcttacccg gtgatttatc
    24481 gccagagcgg tggtagcaag gcagcgcgct tgcagcgacc agatatgcag agggatgggt
    24541 gatttattca gttgccaaac ccgctggagt attgagataa ttttcagtct gactctcgca
    24601 atatcttatg aggtttcagt tcatgtcctg cggcgctctc tgagcgaagc gggtttatca
    24661 ttaacgaata gtcttagtag taccgaaaaa atggcagaga aacgcaatat ctttctggtt
    24721 gggcctatgg gtgccggaaa aagcactatt gggcgccagt tagctcaaca actcaatatg
    24781 gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt gggctgggtt
    24841 ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat caatgagttg
    24901 accgagaaac agggtattgt gctggctact ggcggtggct ctgtgaaatc ccgtgaaacg
    24961 cgtaaccgtc tttccgctcg tggcgttgtc gtttatcttg aaacgaccat cgaaaagcaa
    25021 cttgcacgca cgcagcgtga taaaaaacgc ccgttgctgc acgttgaaac accgccacgt
    25081 gaagttctgg aagcgttggc caatgaacgc aatccgctgt atgaagagat tgccgacgtg
    25141 accattcgta ctgatgatca aagcgctaaa gtggttgcaa accagattat tcacatgctg
    25201 gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat taaggtggat
    25261 gtcgcgttat ggagaggatt gttgttactc tcggggaacg tagttaccca attaccatcg
    25321 catctggttt gtttaatgaa ccagcttcat tcttaccgct gaaatcgggc gagcaggtca
    25381 tgttggtcac caacgaaacc ctggctcctc tgtatctcga taaggtccgc ggcgtacttg
    25441 aacaggcggg tgttaatgtc gatagcgtta tcctccctga cggcgagcag tataaaagcc
    25501 tggctgtact cgataccgtc tttacggcgt tgttacaaaa gccgcatggt cgcgatacta
    25561 cgctggtggc gcttggcggc ggcgtagtgg gcgatctgac cggcttcgcg gcggcgagtt
    25621 atcagcgcgg tgttcgtttc attcaagtcc cgacgacgtt actgtcgcag gtcgattcct
    25681 ccgttggcgg caaaactgcg gtcaaccatc ccctcggtaa aaacatgatt ggcgcgttct
    25741 accagcctgc ttcagtggtg gtggatctcg actgtctgaa aacgcttccc ccgcgtgagt
    25801 tagcgtcggg gctggcagaa gtcatcaaat acggcattat tcttgacggt gcgtttttta
    25861 actggctgga agagaatctg gatgcgttgt tgcgtctgga cggtccggca atggcgtact
    25921 gtattcgccg ttgttgtgaa ctgaaggcag aagttgtcgc cgccgacgag cgcgaaaccg
    25981 ggttacgtgc tttactgaat ctgggacaca cctttggtca tgccattgaa gctgaaatgg
    26041 ggtatggcaa ttggttacat ggtgaagcgg tcgctgcggg tatggtgatg gcggcgcgga
    26101 cgtcggaacg tctcgggcag tttagttctg ccgaaacgca gcgtattata accctgctca
    26161 agcgggctgg gttaccggtc aatgggccgc gcgaaatgtc cgcgcaggcg tatttaccgc
    26221 atatgctgcg tgacaagaaa gtccttgcgg gagagatacg cttaattctt ccgttggcaa
    26281 ttggtaagag tgaagttcgc agcggcgttt cgcacgagct tgttcttaac gccattgccg
    26341 attgtcaatc agcgtaacaa caagaaaggt caggccgctt atcaagcggt ctattagctt
    26401 caggttaatt gcaacgtggt aagcattaac cttttagtgg ggtgttaaat ggatgaattc
    26461 aaaccagaag acgagctgaa acccgatccc agcgatcgtc gtactggtcg ttctcgtcaa
    26521 tcttctgaac gttctgagcg tactgaacgt ggcgaaccgc agatcaattt tgatgatatt
    26581 gaacttgatg acactgacga tcgccgtccg actcgtgcgc aaaaagagcg caatgaggaa
    26641 ccggaaatcg aagaagaaat tgacgaatcc gaagatgaaa ccgtggatga agagcgcgta
    26701 gagcgtcgtc cgcgtaagcg caaaaaagca gccagtaaac ccgcttctcg tcagtatatg
    26761 atgatgggcg tcggcattct ggttctactg ctgttgatca tcggtatcgg ttctgcgcta
    26821 aaagccccct cgacctcttc cagcgatcaa accgcgtctg gcgagaagag tattgatctt
    26881 gcaggcaatg cgaccgatca ggcgaatggg gtgcagccag cgccgggaac cacgtctgcg
    26941 gaaaatactc agcaggatgt ttctctgcca ccgatctctt ctacgccgac tcaagggcaa
    27001 accccggcgg caacggatgg tcaacaacgt gttgaagtgc agggtgacct gaacaatgcg
    27061 ctgacccagc cacaaaatca gcaacagttg aacaatgtgg cggtcaattc cacattgccg
    27121 accgaacccg cgactgtcgc gcctgttcgc aatggcaatg catcgcgtga cacggcgaaa
    27181 acgcaaaccg ctgaacgtcc gtccactacg cgcccagctc gtcagcaggc ggtgattgaa
    27241 ccgaaaaaac cgcaagcaac cgtgaaaacg gagccgaagc cggtagcaca gacgccgaag
    27301 cgtactgaac cagctgcccc tgtggcgagc acgaaggcac cgactgcgac ttctacgcca
    27361 gcaccaaaag agacggcgac tacggctcca gtacagacgg catccccggc gcaaaccacg
    27421 gcaacaccag ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa atcggcaccg
    27481 tccagccatt acactctgca gctgagcagt tcctctaact acgacaacct gaacggttgg
    27541 gcgaagaaag agaatctgaa aaactacgtt gtctatgaaa cggcgcgtaa tggtcagccg
    27601 tggtatgtcc tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa agcggtatct
    27661 acattgccag cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg tcaggtacag
    27721 gccgatctga agtaatcaag gttatctccc gcaatggttt atcgttgcgg gagctgcctg
    27781 aagcgctgga tgctgtcgga gctttctcca cagccggaga aggtgtaatt agttagtcag
    27841 catgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga
    27901 tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc
    27961 ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat
    28021 cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct
    28081 gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa
    28141 aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa
    28201 cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc gctacaaaaa
    28261 aacctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt
    28321 ctattgtgag tcttacgccg atagcatggc gcgcgcagat gatgcatccg tcgtctattg
    28381 cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag
    28441 ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca
    28501 tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc
    28561 aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa
    28621 ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt
    28681 ctcaaggaga agcggatgaa acagtatttg attgccccct caattctgtc ggctgatttt
    28741 gcccgcctgg gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt cgtgcatttt
    28801 gacgtcatgg ataaccacta tgttcccaat ctgacgattg ggccaatggt gctgaaatcc
    28861 ttgcgtaact atggcattac cgcccctatc gacgtacatc tgatggtgaa acccgtcgat
    28921 cgcattgtgc ctgatttcgc tgccgctggt gccagcatca ttacctttca tccagaagcc
    28981 tccgagcatg ttgaccgcac gctgcaactg attaaagaaa atggctgtaa agcgggtctg
    29041 gtatttaacc cggcgacacc tctgagctat ctggattacg tgatggataa gctggatgtg
    29101 atcctgctga tgtccgtcaa tcctggtttc ggcggtcagt ctttcattcc tcaaacactg
    29161 gataaactgc gcgaagtacg tcgccgtatc gacgagtctg gctttgacat tcgtctggaa
    29221 gtggacggtg gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg cgcggatatg
    29281 ttcgtcgccg gttcggcaat cttcgaccag ccagactaca aaaaagtcat tgatgaaatg
    29341 cgcagtgaac tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg gcgtcgcttt
    29401 tgatcttgat ggtacgctgg tcgacagtgc tcccggtctt gctgctgcgg tagatatggc
    29461 gctgtatgcg ctggagttgc ccatcgcagg tgaagaacgc gttattacct ggattggtaa
    29521 cggcgcagat gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac gtgcgactca
    29581 gcgtaaaaca atgggtaaac cgcccgttga tgacgacatt ccggcagaag aacaggtacg
    29641 tattctgcgt aaactgttcg atcgctacta tggcgaggtt gccgaagagg ggacgttttt
    29701 gttcccgcac gttgccgata cgttgggcgc gttgcaggct aaaggcctgc cgctaggcct
    29761 ggtcaccaac aaaccgacgc cgttcgtcgc accgctgctc gaagccttag atattgccaa
    29821 atactttagc gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc atccggaccc
    29881 gctgttactg gtggctgagc ggatgggaat tgccccacaa cagatgctgt ttgtcggcga
    29941 ctcacgcaat gatattcagg cggcaaaagc ggcaggttgc ccatcagttg gcttaaccta
    30001 cggatataac tacggcgagg ctatcgatct cagccagcct gatgtaattt atcagtctat
    30061 aaatgacctt ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat cgaaaaatga
    30121 ctaagcccat cgtttttagt ggcgcacagc cctcaggtga attgaccatt ggtaactaca
    30181 tgggtgcgct gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt tactgtatcg
    30241 ttgaccaaca cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa gcgacgctgg
    30301 atacgctggc cttgtatctg gcttgtggta tcgatcctga gaaaagcacc atttttgttc
    30361 agtcccacgt accagaacat gcgcagttag gctgggcact gaactgttat acctacttcg
    30421 gcgaactgag tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc gagaacatca
    30481 acgctggtct gtttgactat ccggtgctga tggctgcgga catcctgctg tatcaaacta
    30541 atctggtacc ggtgggtgaa gaccagaaac agcacctgga actgagtcgc gatatcgccc
    30601 agcgtttcaa cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt attccgaaat
    30661 ctggcgcgcg cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag tctgacgata
    30721 accgcaataa cgttatcggc ctgctggaag atccgaaatc ggtagtgaag aaaatcaaac
    30781 gcgcggtcac tgactccgac gagccgccgg tagttcgcta cgatgtgcag aacaaagcgg
    30841 gcgtttccaa cctgctggat atcctttctg cggtaacggg ccagagcatc ccagaactgg
    30901 aaaaacagtt cgaaggcaag atgtatggtc atctgaaagg tgaagtggct gatgccgttt
    30961 ccggtatgct gactgaattg caggaacgct atcaccgttt ccgcaacgat gaagccttcc
    31021 tgcaacaggt gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc cgtacgctga
    31081 aagcggtgta cgaagcgatt ggttttgtgg cgaagccgta atttaacatt aatgccaaaa
    31141 accggggaaa cccggttttt ttatcctcat ttgcaataat ccgaaaaaat gtgaagcgcc
    31201 tcgccgtttc cacatctcgt cgttgccact tatcttcatt ttaatgaaga tataaatatt
    31261 cattttattg aaaatatatt atgcgtcgaa cgtttatcaa aaaagaaggc gtcgtcatca
    31321 cgacgctggc ccgttatttg ttgggtgaaa aatgcggtaa tcgactgaaa accatagatg
    31381 agctggcaac tgaatgccgt tcatcggttg gcctgacgca ggccgcgttg aaaacgttgg
    31441 aatcaagcgg agcgatacgg attgaacgcc gtgggcgcaa tggcagttat ctggtcgaga
    31501 tggataacaa agcattgctg agtcatgtgg atatcaacaa cgtggtatgt gcgatgcctt
    31561 tgccctatac ccgcttgtac gaaggtttag cgagcgggct gaaagctcag tttgatggca
    31621 ttccttttta ctatgcgcat atgcgcggcg cggatattcg cgtggagtgt ttacttaacg
    31681 gcgtgtatga catggcggtg gtttcccgac tggcggcgga aagttatctc acgcaaaaag
    31741 ggttatgcct cgcgctggag ttggggccgc acacctacgt tggcgagcac cagttgattt
    31801 gccgtaaagg cgagtccgca aacgtgaagc gcgtggggct ggataaccgt tcggcggatc
    31861 agaaaatcat gaccgatgtc ttttttggcg atagtgatgt ggaacgtgtc gatctctctt
    31921 atcacgagag tttacaacgc attgttaaag gcgatgtcga tgcggttatc tggaacgtgg
    31981 tggcggaaaa cgaactgacc atgctgggat tagaggcgac gccgctcaca gacgatccgc
    32041 gatttttaca ggccaccgaa gccgtggtcc tgacgcgagt cgatgattac ccaatgcaac
    32101 aactgctgcg tgccgttgta gataaacacg ccctgctcgc ccatcaacaa cgggtagtga
    32161 gtggggaaca ggaaccgagt tattaaacga aaggcatctg atatggaaac cagactcaac
    32221 ctgctttgcg aggcaggcgt tattgataag gacatctgca aaggcatgat gcaggtcgtc
    32281 aacgtactgg aaacagagtg ccatctgccg gtgcgcagtg agcaaggaac gatggcgatg
    32341 acacatatgg cgagtgcact gatgcgcagt cgccgtggtg aagaaataga gccgctggat
    32401 aacgaattgc tggcagagct ggcgcaatcc agccactggc aagccgttgt gcaattgcat
    32461 caggtgttgt tgaaggaatt cgcactggaa gttaacccgt gtgaagaagg ctatttgctg
    32521 gccaaccttt atggattatg gatggctgct aacgaagagg tttgaagctc ccgacaaatc
    32581 cacgtcgcac aacctaaata ttcaaaaaaa tgaatattta attcaaggaa cagaaacgag
    32641 gcaacaagat gtttgtagaa gcattgaaac gccagaaccc ggcgctgatt tccgccgcac
    32701 taagtttgtg gcagcagggc aagatcgccc cggacagctg ggtgatcgac gtggatcagg
    32761 tactggaaaa cggtaagcga ctgattgaga cggcgcggct ttacggcatt gaactgtatc
    32821 tgatgaccaa gcagtttggc cgtaacccat ggctggcgga aaaattgctg gcattaggtt
    32881 acagcggcat tgtggcggtg gattacaaag aggcgcgagt catgcgccgc gctggtttgt
    32941 cggtggcaca tcaggggcat ctggtacaaa tcccttgtca tcaggttgct gacgccgttg
    33001 aacagggcac cgacgtcatc accgtgttta ctctcgacaa agcgcgggaa gtttctgcgg
    33061 cagcggtgaa ggccggacgt gtgcagtttg tgctgctcaa agtttatagc gacgatgatt
    33121 ttctttatcc ggggcaggag agcggttttg tacagcattc gctgcacgag gtggttgctg
    33181 aaattaagaa actgccgggg ctgcatttag ccggacttac ccatttccct tgcctgcttt
    33241 gggatgaggc tgccggaaaa gttttgccga caccgaatct tcacacgctg atacaggcac
    33301 gggatcaact ggcgaaatct ggtattgcac ttgagcaact gaacgcgcct tcagcgacca
    33361 gctgcacttc gctgccatta ctggcgtaat acggtgtgac tcatgccgaa cccggtcatg
    33421 cgctgacggg cactattccg gcaaaccagc agggcgatca gtctgaacgt atcgcgatgc
    33481 tctggttgag tgaaatctcc catcatttcc gtggcgacag ctactgctac ggcggcggtt
    33541 actaccgtcg tggtcatgcg caacatgcgt tggtgtttac gccagaaaat caaaagatta
    33601 ctgaaaccaa tctcaaaact gtggatgaca gcagtatcga ctacaccctg tcgctggcag
    33661 gcgagtttcc ggtaagcagt gcagtggtgc tctgtttccg cacgcagatt tttgtcaccc
    33721 gtagcgatgt ggtgctggtg tccggtattc accgtggcga accgaaaatc gttggtcgtt
    33781 atgacagtct tggaaactct ctgggggcgt aatggcgcga tttgtggtgt tagtgattga
    33841 tagctttggc gtaggggcaa tgaaagatgt cacgctggtg cgtccgcaag atgcgggagc
    33901 gaatacatgt ggtcacatcc tgagccagtt gccgcatttg cagctaccaa cgctggagaa
    33961 gctggggcta atcaacgcat tgggttatgc gccaggcgat atgcagccgt cagattccgc
    34021 aacctggggc gtggcagagc tgcaacatga aggtggcgat acctttatgg ggcatcagga
    34081 aattttaggc acgcgcccgt taccgccgct gcggatgcct tttcgcgatg tgattgaccg
    34141 tgttgagcag gcattagtat ccgccggttg gcaggtggag cgccgtggcg atgaactgta
    34201 atttctgtgg gtcaatcagg cggttgcgat tggcgataat ctcgaggcgg atttaggcca
    34261 agtctataac attaccgcca atctctctgt gatctctttt gacgacgcaa tcaaaattgg
    34321 tcgtatcgtg cgtgagcagg tacaggtcgg tcgggtcatt acatttggtg gcctgttaac
    34381 cgacagtcaa cgcattctcg atgccgcaga aagcaaagaa gggcgcttta ttggtatcaa
    34441 tgcgccgcgt tctggcgctt atgacaacgg cttccaggtc gtgcatatgg gctacggtgt
    34501 cgataaaaaa gtgcaggtgc cgcaaaaact gtatgaggcg ggcgtgccaa ccgtgctggt
    34561 gggtaaggtg gcagatatcg tcagcaatcc ttatggcgtg agctggcaaa acctggtgga
    34621 tagccagcgg attatggata tcaccctcga cgaatttaac acccatccga cggcgtttat
    34681 ttgcaccaat atccaggaaa ccgacctcgc tggtcatgca gaagacgtcg cacgttatgc
    34741 cgaacgtttg caggtcgttg accgtaacct tgcccggctt gttgatgcga tgcagccaga
    34801 tgattgcctg gtcgtgatgg cggatcacgg caacgatccg accattggtc acagccacca
    34861 tacccgcgaa gtggtgccgc tgctggttta tcagcaaggc ctggctgcta cgcagctcgg
    34921 tgtgcgcacc acgctttctg atgtgggagc taccgtgtgt gaatttttcc gtgcgccacc
    34981 gccacaaaat ggtcgctctt ttctttcctc cctccggttt gcaggagaca ccctatgagt
    35041 tttgatccga cgggttacac cctggcccat gagcatctgc atattgatct ctccggcttt
    35101 aaaaacaacg tggactgccg ccttgatcag tatgcgttca tttgccagga gatgaacgac
    35161 ctgatgaccc ggggtgtacg taatgtgatt gagatgacca accgttacat ggggcgcaat
    35221 gcgcaattta tgctcggtgt aatgcgcgaa accgggatca acgtggtggc ctgtactggt
    35281 tattaccagg atgcgttttt cccggaacat gtggtgaccc gcagcgtgca ggaactggcg
    35341 caggagatgg tcgatgaaat tgaacagggt atcgatggca cggagctgaa agccgggatc
    35401 atcgcggaga tcggctccag cgaaggaaag attacgccgc tggaagagaa ggtatttatt
    35461 gccgctgcgc tggcgcataa ccagaccgga cgcccgatct ccacgcatac gtcgttcagc
    35521 accatggggc tggaacaact ggctctgttg caagcccacg gagttgatct ttcgcgcgtc
    35581 accgttggtc actgcgatct gaaagacaac ctcgacaaca ttttgaagat gatcgatctc
    35641 ggcgcgtacg tgcagttcga caccattggt aagaacagtt actacccgga cgaaaagcgc
    35701 attgcgatgc tccacgcgct acgtgaccgt gggttgctga accgcgtcat gctgtcgatg
    35761 gatattacgc gccgctccca tttaaaagcc aatggtggtt atggctatga ctatttattg
    35821 accaccttta ttccgcaatt gcgccagtca ggattcagtc aggccgatgt ggatgtgatg
    35881 ttacgtgaaa ctccctctca atttttccaa taaggacaga ctcatgaaaa agattggcgt
    35941 tgcaggctta cagcgtgagc agattaaaaa aaccattgaa gcgacggcta ctggcagttt
    36001 tgaagttttc attcacaacg acatggaagc ggcaatgaaa gtgaaatccg ggcaactgga
    36061 ttactacatc ggtgcgtgta ataccggtgc gggcgcggca ttgtcgattg ccatcgcggt
    36121 gattggctat aacaaaagtt gcaccattgc caaaccaggc attaaagcga aagacgagca
    36181 tatcgccaaa atgatcgctg aaggaaaagt ggcgtttggc ctttccgttg agcacgtcga
    36241 acacgcgatt ccgatgctga ttaaccatct gaaataaaag gcacgactat ggatctgtat
    36301 attcagatta tcgtggtggc gtgcctgacg ggtatgacat cgcttctggc gcatcgctcg
    36361 gcggctgttt ttcatgacgg tatccgcccg atcctgccgc aactgattga aggctatatg
    36421 aaccgtcgcg aggcggggag tatcgctttt ggtctgagca ttggttttgt ggcctcggtg
    36481 gggatctctt ttaccctgaa aaccgggctg ctcaacgcat ggttactctt tcttcctacc
    36541 gatatcctcg gcgtactggc gataaacagc ctgatggcgt ttggtcttgg cgctatctgg
    36601 ggcgtgttga tccttacttg cctgttgcca gtaaaccagc tgctgaccgc gctgccggtg
    36661 gatgtattag gtagcctcgg ggaattaagc tcgccggtgg tttctgcttt tgcactgttc
    36721 ccgctggtgg cgattttcta ccagtttggc tggaagcaaa gtctggtcgc cgccgtggtt
    36781 gttctgatga cccgtgtggt agtcatgcgc tatttcccac atcttaaccc tgaatccatc
    36841 gaaatcttta ttggcatggt gatgctgctg gggatcgcga taactcacga cctgcgtcat
    36901 cgtgatgaaa atgatattga tgccagcggg ctttcggtgt ttgaagaacg cacgtcgcgg
    36961 attatcaaaa acttacccta tatcgccatc gtgggagcat tgattgccgc cgttgccagc
    37021 atgaagattt tcgccggcag tgaagtgtcg atcttcactc tggagaaagc gtactccgca
    37081 ggcgtaacgc cggaacaatc gcaaacgctg atcaaccaag cggcactggc agaatttatg
    37141 cgcgggctgg gttttgtacc gttgattgcc accaccgcct tagcaacggg tgtgtatgca
    37201 gttgcgggct ttacctttgt ttatgcggtg ggctatctct cgccgaatcc gatagttgca
    37261 gcggtattag gcgcagtggt tatttcggcg gaagtcttgc tgcttcgttc gatcggcaaa
    37321 tggctgggac gctacccgtc ggtgcgtaat gcgtcggata acatccgtaa cgccatgaat
    37381 atgctgatgg aagtggcgct gctggtcggt tcgattttcg cagcaattaa gatggcgggt
    37441 tataccggat tctctatcgc ggttgccatt tacttcctca acgaatccct gggccgtccg
    37501 gtacagaaaa tggcggcacc ggtcgtggca gtaatgatca ccggtattct gctgaatgtt
    37561 ctttactggc ttggcctgtt cgttccggct taaggaaggc acctatgaag acgtttcctc
    37621 tgcaaagtct gacgattatt gaggcgcagc aaaagcagtt tgcgctggtg gatagcattt
    37681 gtcgccattt ccccggcagc gagtttctta ctggcggtga tttaggctta acgccaggac
    37741 tgaatcaacc gcgcgttacc cagcgtgtgg aacaggtgct ggctgatgca tttcacgcac
    37801 aggctgcggc gctggtgcag ggtgcgggta ctggcgcgat tcgtgcggcg ttggcggctt
    37861 tgctcaaacc ggggcagcgt cttctggtac atgatgcgcc tgtttacccg acgacacagg
    37921 tcattattga gcagatgggg ctgacgctta ttactgctga tttcaatgac ctgtcggcac
    37981 tgaagcaggt cgtcgacgag caactaccgg atgcggcgct ggtgcagcat acgcgccagc
    38041 agccgcagga cggctacatt ctggcagatg tgctggcaac attgcgctcg gctggcgttc
    38101 ctgcattaac cgatgacaac tatgcggtga tgaaagtggc gcgaattggc tgcgaatgcg
    38161 gggcaaatgt ctcgactttt tcctgcttca agctgtttgg gccggagggt gttggcgcgg
    38221 tggtaggcga tgccgatgtt attagtcgga ttcgctccac gctctattcc ggcggcagcc
    38281 agattcaggg cgctcaggcg ctggaagtct tgtgtgggct ggtgtttgcg ccagtgatgc
    38341 acgcggtgca ggcgggggta tctgaacggt tgctggcttt gcttaacggt ggtgcggtgc
    38401 cggaagtgaa aagcgcggtg attgctaatg cgcagtcgaa ggtgttgatt gtcgagtttc
    38461 atcagccgat tgccgccaga gtgctggaag aggcgcaaaa gcgcggtgcc ttgccttacc
    38521 cggtgggtgc agagtcgaaa tatgaaatcc cgccgctctt ttatcgcctt tccggaacgt
    38581 ttcgccaggc gaatccacaa tcagaacatt gtgcgattcg cattaacccg aatcgcaacg
    38641 gtgaagagac ggtgctgcgg attttgcgtg agagtattgc cagtatttaa tgctggatag
    38701 caccgtcaag ctaaattccg tactgaacgg tcccctcgcc cctttgggga gagggttagg
    38761 gtgaggggaa cgtacgggat gtaaaattca acgcgccagc atcgccggat gcgatgctgg
    38821 cgcatcttat ccggcctacg gttaatgtct tttattatca atcgttaagg tgatgcgatt
    38881 ggcgcggcag aagagttcgg agacgtgcac cggtttgtca ttctgatcaa aggcgatttt
    38941 gctgatgcga aacaacggtt cgcccagttc gcatttcagc cattttgcct gcgggcgggt
    39001 ggcggcaaag atatcgatgg tctttttgtc gctgaccact cgcgtgtcaa aacgctcctg
    39061 aaataactga taggtggagc ttccttcgac gtaaatctca tcaaagtcag gataacgcga
    39121 cagcgggatc catgaactat caataaacag cggctcttta tcgagataca tcacccggca
    39181 gagatggaac acttcgctgt ttccggggat gttcagcttt tcgcaaaacg gcgcggcgct
    39241 gacccgttcc tgttcgatca ctttctcttt cgtcgccttg ccttgtgaga cgccaaaatc
    39301 ggtaaaacca ctgacagtaa gcagggcgtt ttcaactttc tggctttgta caaaggtgcc
    39361 tttgccctgc cagcggatca gtacgccgtc tgccactaag tcgctgatag ctttgcgaat
    39421 agtaatgcgg ctgacgttat attgcgtaca aagctcgttt tcggtaggga tctgttgccc
    39481 ggcctggtaa accccctgcg cgatatcatc cagcagccgc tggcggacgg tagcgtagag
    39541 gagttgatga gagtagcggt ccgtagctga cataatcgac cctgaaataa agaatgaatt
    39601 acacggatta tagggtcgtt ttccggcaag taacagcacg ttggcgcgga gatgctcacg
    39661 ccaacgttat acctaccagg caccgtgata ctgaatggtt ttcgccgcgc acgccgttcc
    39721 ctgcgccatc gcctgcggta atgtcatccc cgcagaccag ccgcaaagga atccggcaat
    39781 gaacgaatct ccggcaccca tggtgtcgat aaccgtcacc ggttcaggag cctgacgcca
    39841 gaactgcgcg ccatcccagg caatgctgcc gttttcaccc agcgtgacaa tcactgttcc
    39901 tgcgccacgg gcaacaatcg ctttcatctt cagacgcagc gcttcgtctt cttgcggtgc
    39961 ggaggcaaag gcaaaatcga gatgcggcac cagtgtctgc cagagcgggc tgtcccactt
    40021 gtcggagaag tcgaaagcgg taagtttgcc cgcagcgtgc agctgtggga atgcgtcttc
    40081 cgcatgtccc cagattgccg cgtgcacaat gtcatactgc gccagccagg cgtaatcctc
    40141 ttcactcagg gcaaagtcgg ccatcacgcc ttcggtgtag tcgccaaaaa cgcgatcatt
    40201 gtcgtgcagt tccacctgag tttgtgcggt aacgccgtgt ttcgtatgga catggctgat
    40261 atcgacgccc atgcgggcga gatcctgctt cagctttgtg ccgtagtcat cgtcacccac
    40321 caaggtaatg catcccggct gtatgccgta gcgagtgcag tacaccgcca cattgaccgc
    40381 attaccgcca gaaaacgctt tattcagttg cgggtagata tcgacgcagt tatcgccgat
    40441 tgtcgccagg gttttcatct ctcatcctcc ggcagcagcg cgcgaaagcg ttccagcgcc
    40501 tggcgggcat agagtctggg ctcgttcata tacatcgtca ccagctccac cgtacagtaa
    40561 ccctcatagc cccgctcaat aatatcgcgc atcagttccc gcagcggcat tttgccttct
    40621 ccaggaatgt aatgcgtgtc gctggccccg tcgctgtcga caatatgcag atgacgataa
    40681 tttatcgccc agtttgtcga aataactcat caccggttcc gcctggacat acggcgcgca
    40741 aatgtcgacc atgctgaaca ggcgcggcga aggcaccagc gccagcgcat gaagcacatc
    40801 attagcatta cacacgacgt tcgattcata cggcgttaac ggttcgagga ttagatccat
    40861 gccgatgttt tccgcgtact cacacagttc gcttaggttc tctgccagcc gtccccagat
    40921 aacattaggt ggcgtgagat agcctgcgtg ggccgcggaa atcagcgtat aacccgcgtt
    40981 catctctttt gccatatcca tcgccagctt gatcatgtcg aggctttcgc gacgcatatg
    41041 ttcatcgccc agcatcatgt tatacggata gccgttggtt tctggcgtat agccgataat
    41101 cggcatctga tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg cttttaagtc
    41161 cggcgcgaac gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac ccagctcgct
    41221 tgcatcacga aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa acatacctgt
    41281 tttcatcggg taactccttg cgtaaatccg gcccgttgcc gggccgggag agggattaat
    41341 attccaccag accaccgtaa tagcggcgtt catccgggtt gtgatctttg taaatagaca
    41401 ggtagtagca gagccactcc attggcacga acatcaggaa cggtgccagc cacgggtgca
    41461 gcccttgcga aatttcggcg taatcgatga cgatcacgtt gtcagtacgc tgtttaacaa
    41521 agttaatggc gcgttcggtg gtgtggcgac tttcatcatt gccgagcagg aacaggaacg
    41581 gaacgcccgg ttcgacaatc tccagcgggc catggcggaa ctctccgctc tcaatcacgc
    41641 agccgtgcgt ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa cccagcggac
    41701 gcagcggacc cgcagcaacg gtataaatca tcggccactg gctggccagt tcaccaagct
    41761 ggcggccttt ttcttcccag gtgcgtacca gatgaccgag cgcattcggc aacggcttga
    41821 gatcgttttt gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc atctccagca
    41881 ccacgctgta gcagagcagc aggtgaattt cccagataca gtcggcctga taatcaatgc
    41941 taaattccgc cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc gcagtgagtg
    42001 cgccgcaggc ccggcccagc tccagcgctt tgattacctc ttccgtttta ccgtagtcag
    42061 aaacgccaat caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg cagaactccc
    42121 agccggaaat ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt ttcgccgttt
    42181 gcgccgcgtt gagtggcgag ccgcaggcaa cgaaataaat acgatcaatg tcgcgcttca
    42241 ccatctcttc cacgatggcg tgcaccagcg gaacgtcatg gctgagaact ttttccactt
    42301 cctgaaccat attttcggtg accagaaagt ccacggtgct tttatcaata tccaacattt
    42361 tattttctcc agataacatt acgacaaagc gttgagctgg cggctacgct tcgcccagaa
    42421 ggcgtaagca ggcagaccag tagcaataac gatgacggcg cagataaggc cgggaatcgg
    42481 tgcccagaca aaggttgagg cgaccagaat gaggcttgac gcaatggcga gggtggtcat
    42541 cagcccgaaa gccggagtac gccacagcgg tttgtaatcg tcgcgtttac gacaccagat
    42601 gatggagccg aaggtgaggg tatttttgaa acacatcacc agggtgaaat aacccagcag
    42661 gctggtgaga tcggaaacga agatgaagaa gatccccagc gccccttgca ggatgatgga
    42721 gacatccggc gtgttgtatt tcggatgcac atggccgaag catttaaaga acaggttgtc
    42781 tttcgccatc gcgtattcca gccgcggctg gtacatcacg cagctggaaa gcgaaccaag
    42841 aatgacgatc atcgccgtga tggcaacaaa gatcccagcg gtgctgccga gtgcggggat
    42901 ccaggtcagg gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga agggcatcag
    42961 gccggaaatc accagcgcca gcagggtgta gagcaccaga accagcagac aggaaccaat
    43021 cagcgctcgt ggcatggttt ttccggggtt tttaatttcg ccggtcatat aacagataga
    43081 ggccatgccg gtatacgacc aactggtggc agagatcccc gccagcagcg ccataaagct
    43141 gcccgttgcg ccaatcgcag tggtggtagg ggcggcaaaa ttctccgctt taaaccagaa
    43201 gatcccaagg ccaatgacga tagtgaacgg gataattttg gcgatggtaa ttagcgtctg
    43261 aaacgctgcg ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga taattaatcc
    43321 ggcggcgata aatttaccga gcaacggatc gataggcgtt aaaaagccaa gattgctgac
    43381 aatcgccagc gccataatcg acaatgacgg cgcatcgttg gcccagaagc tggcccagcc
    43441 ggagaggaaa gccagcggtc ggcttccggc atttttcaga taaacataat ctgcgccatt
    43501 ttccggataa gcggtggata gttccgcata gacgcacatt tgcgggatca caattaaccc
    43561 gccaatgaca aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg ccacttcacc
    43621 cacagataca aaaataccgg agccgacggt tgtcccgacg gcgattgcaa gaacggccca
    43681 aaatccgagc ttgcgttgga gttcctggct tccc
//
LOCUS       DAJVYM010000014        43478 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVYM010000014 DAJVYM010000000
VERSION     DAJVYM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.014"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..77)
                     /locus_tag="OQS60_000753"
     tRNA            complement(1..77)
                     /locus_tag="OQS60_000753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(81..173)
                     /locus_tag="OQS60_000754"
     tRNA            complement(81..173)
                     /locus_tag="OQS60_000754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Ser,seq:gct)
     gene            complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQS60_000755"
     CDS             complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQS60_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS2386595.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQS60_000756"
     CDS             complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQS60_000756"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS2386596.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQS60_000757"
     CDS             complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQS60_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS2386597.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQS60_000758"
     CDS             complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQS60_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS2386598.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQS60_000759"
     CDS             complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQS60_000759"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS2386599.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQS60_000760"
     CDS             complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQS60_000760"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS2386600.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7519..8082
                     /gene="srlA"
                     /locus_tag="OQS60_000761"
                     /gene_synonym="gutA"
     CDS             7519..8082
                     /gene="srlA"
                     /locus_tag="OQS60_000761"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS2386601.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8079..9038
                     /gene="srlE"
                     /locus_tag="OQS60_000762"
                     /gene_synonym="gutE"
     CDS             8079..9038
                     /gene="srlE"
                     /locus_tag="OQS60_000762"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS2386602.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9049..9420
                     /gene="srlB"
                     /locus_tag="OQS60_000763"
                     /gene_synonym="gutB"
     CDS             9049..9420
                     /gene="srlB"
                     /locus_tag="OQS60_000763"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS2386603.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9424..10192
                     /gene="srlD"
                     /locus_tag="OQS60_000764"
                     /pseudo
     CDS             9424..10192
                     /gene="srlD"
                     /locus_tag="OQS60_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10298..10657
                     /gene="gutM"
                     /locus_tag="OQS60_000765"
     CDS             10298..10657
                     /gene="gutM"
                     /locus_tag="OQS60_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS2386604.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10724..11497
                     /gene="srlR"
                     /locus_tag="OQS60_000766"
                     /gene_synonym="gutR"
     CDS             10724..11497
                     /gene="srlR"
                     /locus_tag="OQS60_000766"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS2386605.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11490..12455
                     /gene="gutQ"
                     /locus_tag="OQS60_000767"
     CDS             11490..12455
                     /gene="gutQ"
                     /locus_tag="OQS60_000767"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS2386606.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQS60_000768"
     CDS             complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQS60_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS2386607.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14153..15592
                     /gene="norV"
                     /locus_tag="OQS60_000769"
     CDS             14153..15592
                     /gene="norV"
                     /locus_tag="OQS60_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS2386608.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15589..16722
                     /gene="norW"
                     /locus_tag="OQS60_000770"
     CDS             15589..16722
                     /gene="norW"
                     /locus_tag="OQS60_000770"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS2386609.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQS60_000771"
     CDS             complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQS60_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS2386610.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQS60_000772"
     CDS             complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQS60_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS2386611.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQS60_000773"
     CDS             complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQS60_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS2386612.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21200..22657
                     /gene="ascF"
                     /locus_tag="OQS60_000774"
     CDS             21200..22657
                     /gene="ascF"
                     /locus_tag="OQS60_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS2386613.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22666..24090
                     /gene="ascB"
                     /locus_tag="OQS60_000775"
     CDS             22666..24090
                     /gene="ascB"
                     /locus_tag="OQS60_000775"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS2386614.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24204..24623)
                     /locus_tag="OQS60_000776"
     CDS             complement(24204..24623)
                     /locus_tag="OQS60_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2386615.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24620..24931)
                     /locus_tag="OQS60_000777"
     CDS             complement(24620..24931)
                     /locus_tag="OQS60_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCS2386616.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25093..25866)
                     /locus_tag="OQS60_000778"
     CDS             complement(25093..25866)
                     /locus_tag="OQS60_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386617.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQS60_000779"
     CDS             complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQS60_000779"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS2386618.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQS60_000780"
     CDS             complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQS60_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS2386619.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQS60_000781"
     CDS             complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQS60_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS2386620.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQS60_000782"
     CDS             complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQS60_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS2386621.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQS60_000783"
     CDS             complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQS60_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS2386622.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQS60_000784"
     CDS             complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQS60_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS2386623.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQS60_000785"
     CDS             complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQS60_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS2386624.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQS60_000786"
     CDS             complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQS60_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS2386625.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQS60_000787"
     CDS             complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQS60_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS2386626.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33953..34303
                     /gene="hypA"
                     /locus_tag="OQS60_000788"
     CDS             33953..34303
                     /gene="hypA"
                     /locus_tag="OQS60_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS2386627.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34307..35179
                     /gene="hypB"
                     /locus_tag="OQS60_000789"
     CDS             34307..35179
                     /gene="hypB"
                     /locus_tag="OQS60_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS2386628.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35170..35442
                     /gene="hypC"
                     /locus_tag="OQS60_000790"
     CDS             35170..35442
                     /gene="hypC"
                     /locus_tag="OQS60_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS2386629.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35442..36563
                     /gene="hypD"
                     /locus_tag="OQS60_000791"
     CDS             35442..36563
                     /gene="hypD"
                     /locus_tag="OQS60_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS2386630.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36560..37570
                     /gene="hypE"
                     /locus_tag="OQS60_000792"
     CDS             36560..37570
                     /gene="hypE"
                     /locus_tag="OQS60_000792"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS2386631.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37644..39721
                     /gene="flhA"
                     /locus_tag="OQS60_000793"
                     /pseudo
     CDS             37644..39721
                     /gene="flhA"
                     /locus_tag="OQS60_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39758..40111)
                     /locus_tag="OQS60_000794"
     CDS             complement(39758..40111)
                     /locus_tag="OQS60_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS2386632.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40188..40322)
                     /locus_tag="OQS60_000795"
     CDS             complement(40188..40322)
                     /locus_tag="OQS60_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386633.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40398..42959
                     /gene="mutS"
                     /locus_tag="OQS60_000796"
     CDS             40398..42959
                     /gene="mutS"
                     /locus_tag="OQS60_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS2386634.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43065..43478
                     /locus_tag="OQS60_000797"
                     /pseudo
     CDS             43065..43478
                     /locus_tag="OQS60_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10293 a        11938 c        11327 g         9920 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga tgcagctttt
      121 gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc accacacgcc
      181 tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt
      241 actttctgag acttataagt caaacaattt ttcccacact tttatcgttt gcacacttca
      301 cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa aacggaggtc
      361 tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg
      421 aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa agatgagacg
      481 cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg gtagatctct
      541 tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg gttgcccttt
      601 acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc aactcgacga
      661 gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc
      721 ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta catgtaagca
      781 gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact tcaacctgac
      841 tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg cattgcctga
      901 cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa cgctgcaggt
      961 aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc acccttgccg
     1021 cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc tgtgacgtcc
     1081 ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag cacgataatt
     1141 gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt cggctcaaca
     1201 ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt actggaaaga
     1261 tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt ttctaactga
     1321 cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc gcctttcagc
     1381 agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat agcgccttct
     1441 ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga gatgatgcgg
     1501 aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc ggtggagaaa
     1561 tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag cgccatcgca
     1621 cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa attgcgacga
     1681 atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat cgcttcgttg
     1741 tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg cgatacatga
     1801 gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg attcagacga
     1861 atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac tttcagagaa
     1921 cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt atcttccacc
     1981 gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc gccggattcc
     2041 gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt gatggcatca
     2101 accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag atggtcatag
     2161 cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc accaaagccg
     2221 ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc agcttcgtca
     2281 actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa gccataggtg
     2341 tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt tgccagctct
     2401 tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc ttcagtcttc
     2461 agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga gcccataacg
     2521 tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag catattgccg
     2581 tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc attggacggc
     2641 attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc
     2701 gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat caacgtgcgg
     2761 aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat acgctccaga
     2821 cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc tgcctggcgg
     2881 ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga
     2941 cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg
     3001 ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg cgcacctttg
     3061 ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc ccagatttcg
     3121 taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg cagggcaaac
     3181 catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt gaaatagtcg
     3241 ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt gtaaccgaca
     3301 ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt ggtagcgcgg
     3361 gaataattac gcttgtcgag tccaaggaac acatccttga actggttcat cccggcgttg
     3421 gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac tacctgatgt
     3481 cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct cttgctcata
     3541 attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg
     3601 ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt aaaataccgt
     3661 atgcgttcag tcggcaaaat ttagccaaat atcctggata tcttccatca gatagccacg
     3721 atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca aaggttcacc
     3781 atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac attcacgcat
     3841 cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac gaatacgtgc
     3901 aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc ggctgtcatc
     3961 gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg ccgtagcatc
     4021 aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc gcagttcttg
     4081 ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc gagcatatgc
     4141 cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa agcaaaaggg
     4201 ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga gtgtgcagcc
     4261 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc tgttcccctc
     4321 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4381 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4441 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4501 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     4561 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     4621 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     4681 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     4741 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     4801 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     4861 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     4921 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     4981 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5041 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5101 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5161 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5221 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5281 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5341 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5401 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5461 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     5521 tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     5581 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
     5641 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     5701 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     5761 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     5821 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     5881 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     5941 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6001 ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc aaaaatttat
     6061 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6121 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6181 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6241 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6301 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6361 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6421 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6481 tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     6541 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     6601 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     6661 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     6721 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     6781 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     6841 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     6901 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     6961 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7021 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt cgatgaactg
     7081 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgaggctc
     7141 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7201 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7261 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
     7321 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
     7381 gctatccttt tattcaaact ttcaaattaa aatatttatc tttcattttg cgatcaaaat
     7441 aacactttca aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
     7501 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
     7561 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
     7621 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
     7681 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
     7741 ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat gccggaaaag
     7801 tacaaaccca gctactacgc ggcagcctct tatagctgcc actcaatgaa tggcctcttt
     7861 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
     7921 ctgaacctgc cacttggccc actggcggtg agctatctgc tggttggtct ggtcaccaac
     7981 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
     8041 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
     8101 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
     8161 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     8221 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     8281 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     8341 ttaatatcca ctcgacgggc aagtccagtc cgctggcgca gtacattgtg gaagatattt
     8401 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     8461 cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc gatggtttac
     8521 tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg
     8581 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc tcggcgctca
     8641 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
     8701 cgagccatcc actaggtctg gtcatgctgg cgctcatctg ctccttcccg ctgctttcac
     8761 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     8821 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     8881 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     8941 ttcgcgtcgg tgtcccttct gtactggtga gccacttttt aaccggcgca ccgactgtac
     9001 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     9061 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     9121 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     9181 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
     9241 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     9301 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     9361 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     9421 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     9481 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     9541 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta tagttttggt
     9601 gctgacgcca ccagcgagca aagcgtcctg gcgctctctc gtggggtaga tgaaatcttt
     9661 gggcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac
     9721 ttccagctcg gcgattttga ccgtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
     9781 ttctcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
     9841 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
     9901 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
     9961 gctcggtaac ctgctgaaat cgcctatgtt ccagtcactg ttaccccaat acgcgaccaa
    10021 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10081 cggctgcgat tatcaggatg tgctgaatat gctgctgctc tatgccagtc ctaaggcgtc
    10141 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttgt aatcaacagc
    10201 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    10261 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    10321 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    10381 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    10441 ctttaaacca cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgtcgacac
    10501 cttgtttatg aaaggactga ccgtcttcgc acgaccgcaa aaaattcccg caattaccgg
    10561 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    10621 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    10681 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    10741 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    10801 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    10861 aggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    10921 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    10981 tagttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    11041 ggttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagtc tgcatatcgt
    11101 caatgcgcta tctgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11161 taaaaaatcg gcctcatttc acggacagct ggcagagaat gccttcgaac atttcagttt
    11221 cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacctttaa
    11281 cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    11341 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    11401 taagctgatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    11461 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    11521 agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    11581 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    11641 gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacatcgg
    11701 ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg
    11761 atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc
    11821 gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    11881 gcctggcggc aaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    11941 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    12001 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccca gccggggcac
    12061 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12121 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12181 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12241 tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga
    12301 caggcggcac cacgttacag gcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    12361 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaatta
    12421 acctgcagga tttctatcag gccgggatta tttaatcctt cattcccaga cgtttcgcca
    12481 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttatgat
    12541 gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    12601 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa
    12661 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    12721 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    12781 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagccccagc cgcaaacgac
    12841 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    12901 gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca
    12961 tcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13021 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13081 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag agtgccgtta tccgccattt
    13141 caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    13201 actcactttc cgccacactt tccggcagtg cagcacagtt gagatagacc agcggattca
    13261 ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    13321 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    13381 gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg
    13441 catcgcctgg catcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    13501 ctcccgccgc cagcgcagca atcagccgta gctcttcgtc gctgaaaaca tcgaactgat
    13561 cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca
    13621 gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat
    13681 cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca
    13741 gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga
    13801 tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca
    13861 gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    13921 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    13981 caatttgact atagatattg tcatatcgac catttgattg atagtcattt tgactactca
    14041 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgtaat
    14101 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    14161 tgtggtgaaa aataacattc attgggttgg tcaacgtgac tgggaagtgc gtgattttca
    14221 cggtacggaa tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga
    14281 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    14341 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    14401 ccacgctggg gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac
    14461 tgccaacgct atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt
    14521 ggtgaaaact ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    14581 gccaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    14641 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    14701 tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag
    14761 ccgcctggta acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    14821 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    14881 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    14941 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    15001 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    15061 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    15121 cgccggtctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    15181 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    15241 gggtttcgaa atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga
    15301 gttatgccgt gaacatggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    15361 gagcacggtg aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg
    15421 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    15481 gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga
    15541 atgctctctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    15601 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    15661 gacgccagta ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg
    15721 gacctcagcc atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg
    15781 ggtgaatttg ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc
    15841 gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    15901 ctggcaactg gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    15961 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    16021 ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    16081 aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa
    16141 gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    16201 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt
    16261 agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca
    16321 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    16381 aatgacgata tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc
    16441 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    16501 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    16561 gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg
    16621 gtggctcgcg gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg
    16681 atgaaagagg catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa
    16741 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccag
    16801 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    16861 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    16921 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    16981 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    17041 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    17101 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg
    17161 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg
    17221 aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    17281 cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg
    17341 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    17401 ctgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    17461 gccacggctg cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    17521 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct
    17581 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact
    17641 gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca
    17701 gattcatttc acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
    17761 attgcggggt aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    17821 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    17881 ccaggcagaa ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa
    17941 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    18001 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    18061 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    18121 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    18181 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    18241 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    18301 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    18361 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    18421 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    18481 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    18541 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    18601 cacggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    18661 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    18721 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    18781 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cggatggtga
    18841 actcagtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    18901 gcggcgggca gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga
    18961 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    19021 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    19081 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    19141 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    19201 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    19261 catcagcgcc gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac
    19321 acaaatcagc gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    19381 gcacaggtca catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct
    19441 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    19501 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    19561 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    19621 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    19681 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacagca
    19741 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    19801 cagttaatgc gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga
    19861 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    19921 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    19981 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    20041 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    20101 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    20161 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    20221 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    20281 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    20341 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    20401 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    20461 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    20521 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    20581 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    20641 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    20701 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt
    20761 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    20821 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    20881 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    20941 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    21001 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    21061 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    21121 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    21181 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    21241 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt
    21301 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    21361 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    21421 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    21481 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    21541 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    21601 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    21661 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    21721 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    21781 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    21841 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    21901 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    21961 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    22021 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    22081 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    22141 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    22201 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    22261 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    22321 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    22381 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    22441 agcgccaggg ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    22501 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    22561 cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt
    22621 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    22681 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    22741 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    22801 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    22861 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    22921 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    22981 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    23041 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    23101 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt
    23161 gaagcatttg atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg
    23221 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    23281 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    23341 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    23401 agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt
    23461 atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa
    23521 ggggtaacca tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    23581 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc
    23641 agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    23701 tggggtattg atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag
    23761 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    23821 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    23881 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc
    23941 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    24001 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    24061 attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct
    24121 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    24181 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    24241 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    24301 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    24361 agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca
    24421 aactcttggt tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga
    24481 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    24541 tctgagtcac tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    24601 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat
    24661 actcggcgtg attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg
    24721 caatcaccct taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa
    24781 aattgtcgag gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct
    24841 taataacccg atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact
    24901 cttcaaatgg cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt
    24961 aaacagccac atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc
    25021 aagttcacaa tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca
    25081 ctctgcaaaa agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg
    25141 ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag
    25201 tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca
    25261 gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc
    25321 ggaatcaaac tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc
    25381 atctacgcca gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc
    25441 ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact
    25501 tggtaaccag ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg
    25561 ccctttgccc gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg
    25621 atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc
    25681 agcaacgccc acttttctaa aacgccaggt ttgctcggca tcataaggca tggtcaggag
    25741 aggtttcagc gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa
    25801 gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat
    25861 attcatttcg ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa
    25921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    25981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    26041 ttcgccaatg tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt
    26101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    26161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    26221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    26281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    26341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    26401 gaatatcatg cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg
    26461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    26521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    26581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    26641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    26701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    26761 tcatcggata cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg
    26821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    26881 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    26941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc
    27001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    27061 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg
    27121 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac caccgctgtt
    27181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    27241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    27301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    27361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga
    27421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    27481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    27541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    27601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    27661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg
    27721 cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt
    27781 tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    27841 aagatgcagc gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca
    27901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc
    27961 gggttctgct ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    28021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    28081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    28141 actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    28201 gggtccaagc taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag
    28261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    28321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    28381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta
    28441 tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga
    28501 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg
    28561 acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca
    28621 acgttactga aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt
    28681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    28741 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    28801 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag
    28861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg
    28921 agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    28981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg
    29041 gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    29101 taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag
    29161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    29221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    29281 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    29341 ttacgcagcg gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc
    29401 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    29461 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    29521 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    29581 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    29641 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    29701 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    29761 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    29821 ctaaaaatgc aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac
    29881 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    29941 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    30001 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    30061 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    30121 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    30181 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    30241 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    30301 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    30361 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    30421 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    30481 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    30541 aggcatccgg gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca
    30601 acacacccgg cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    30661 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    30721 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    30781 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    30841 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    30901 agcggtaatc accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga
    30961 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    31021 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    31081 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    31141 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    31201 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    31261 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    31321 ggcaacaaac gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc
    31381 ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    31441 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    31501 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    31561 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    31621 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    31681 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    31741 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    31801 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    31861 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    31921 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    31981 ttgcatacgc atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca
    32041 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    32101 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    32161 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    32221 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    32281 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    32341 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    32401 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    32461 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    32521 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    32581 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    32641 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    32701 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    32761 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    32821 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    32881 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    32941 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    33001 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    33061 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    33121 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    33181 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    33241 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    33301 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    33361 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    33421 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    33481 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    33541 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    33601 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    33661 gtagatgtgc cactggtcct gtaggcgaca atgccgctgt gcgatgtaat cggctttctc
    33721 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    33781 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    33841 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    33901 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    33961 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    34021 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    34081 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    34141 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    34201 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    34261 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    34321 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    34381 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    34441 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    34501 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    34561 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    34621 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    34681 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    34741 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    34801 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    34861 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    34921 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    34981 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    35041 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    35101 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    35161 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    35221 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    35281 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    35341 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    35401 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    35461 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    35521 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    35581 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    35641 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    35701 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    35761 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    35821 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    35881 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    35941 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    36001 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    36061 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    36121 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    36181 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    36241 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    36301 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    36361 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    36421 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    36481 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    36541 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    36601 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    36661 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    36721 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    36781 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    36841 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    36901 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    36961 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    37021 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    37081 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    37141 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    37201 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    37261 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    37321 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    37381 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    37441 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    37501 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    37561 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    37621 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    37681 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    37741 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    37801 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata
    37861 tgaagacgaa actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac
    37921 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct
    37981 ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
    38041 tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct
    38101 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa
    38161 caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat
    38221 taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
    38281 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
    38341 caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag
    38401 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
    38461 gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta
    38521 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
    38581 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct
    38641 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
    38701 ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa
    38761 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
    38821 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
    38881 gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat
    38941 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
    39001 tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga
    39061 taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
    39121 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
    39181 ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga
    39241 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
    39301 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
    39361 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
    39421 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
    39481 cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
    39541 tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct
    39601 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
    39661 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
    39721 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac
    39781 gatatttttc cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta
    39841 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    39901 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc
    39961 cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg
    40021 gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    40081 gtttttcacg agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta
    40141 tattacaact taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac
    40201 gcaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc
    40261 ggaaccagca tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc
    40321 atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg
    40381 gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc
    40441 agcagtatct caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg
    40501 atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac
    40561 tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg
    40621 cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac
    40681 aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta
    40741 cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg
    40801 ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt
    40861 ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc
    40921 cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg
    40981 gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc
    41041 tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt
    41101 gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata
    41161 ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc
    41221 gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc
    41281 tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag
    41341 tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca
    41401 ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc
    41461 tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac
    41521 tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg
    41581 agaagatggg cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac
    41641 cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg
    41701 agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc
    41761 gtgaacgtac cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca
    41821 ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc
    41881 tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca
    41941 cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc
    42001 tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc
    42061 tggtgaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata
    42121 aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc
    42181 catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc
    42241 cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct
    42301 acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta
    42361 cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga
    42421 ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga
    42481 tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc
    42541 tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc
    42601 taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag cacggcgaca
    42661 ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac ggtctggcgg
    42721 ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa aaactgcgtg
    42781 agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg cagatgtctt
    42841 tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg
    42901 attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat
    42961 aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa
    43021 aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct
    43081 atcaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg
    43141 aatatcagtt attacaatcc cgttaacatc aactctcttt ttaccctgaa accgacttac
    43201 ttatttccac aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc
    43261 taaaccaacc ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat
    43321 tcgaaactgg cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa
    43381 atgattcaga gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata
    43441 ttattgaaat cattaatgac aacataaaat atgccatc
//
LOCUS       DAJVYM010000015        40901 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVYM010000015 DAJVYM010000000
VERSION     DAJVYM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.015"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            217..1881
                     /gene="asnB"
                     /locus_tag="OQS60_000798"
     CDS             217..1881
                     /gene="asnB"
                     /locus_tag="OQS60_000798"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS2386635.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2261..2337
                     /locus_tag="OQS60_000799"
     tRNA            2261..2337
                     /locus_tag="OQS60_000799"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2295..2297,aa:Met,seq:cat)
     gene            2346..2430
                     /locus_tag="OQS60_000800"
     tRNA            2346..2430
                     /locus_tag="OQS60_000800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2380..2382,aa:Leu,seq:tag)
     gene            2454..2528
                     /locus_tag="OQS60_000801"
     tRNA            2454..2528
                     /locus_tag="OQS60_000801"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2486..2488,aa:Gln,seq:ttg)
     gene            2563..2637
                     /locus_tag="OQS60_000802"
     tRNA            2563..2637
                     /locus_tag="OQS60_000802"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2595..2597,aa:Gln,seq:ttg)
     gene            2653..2729
                     /locus_tag="OQS60_000803"
     tRNA            2653..2729
                     /locus_tag="OQS60_000803"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2687..2689,aa:Met,seq:cat)
     gene            2778..2852
                     /locus_tag="OQS60_000804"
     tRNA            2778..2852
                     /locus_tag="OQS60_000804"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2810..2812,aa:Gln,seq:ctg)
     gene            complement(3006..4181)
                     /gene="ubiF"
                     /locus_tag="OQS60_000805"
     CDS             complement(3006..4181)
                     /gene="ubiF"
                     /locus_tag="OQS60_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS2386636.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4327..5751
                     /gene="miaB"
                     /locus_tag="OQS60_000806"
     CDS             4327..5751
                     /gene="miaB"
                     /locus_tag="OQS60_000806"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS2386637.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5904..6944
                     /locus_tag="OQS60_000807"
     CDS             5904..6944
                     /locus_tag="OQS60_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS2386638.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            6941..7408
                     /gene="ybeY"
                     /locus_tag="OQS60_000808"
     CDS             6941..7408
                     /gene="ybeY"
                     /locus_tag="OQS60_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS2386639.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7498..8376
                     /gene="corC"
                     /locus_tag="OQS60_000809"
     CDS             7498..8376
                     /gene="corC"
                     /locus_tag="OQS60_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS2386640.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8401..9939
                     /gene="lnt"
                     /locus_tag="OQS60_000810"
     CDS             8401..9939
                     /gene="lnt"
                     /locus_tag="OQS60_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS2386641.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10017..11894)
                     /locus_tag="OQS60_000811"
     CDS             complement(10017..11894)
                     /locus_tag="OQS60_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS2386642.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12349..13257
                     /gene="gltI"
                     /locus_tag="OQS60_000812"
     CDS             12349..13257
                     /gene="gltI"
                     /locus_tag="OQS60_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS2386643.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13427..14167
                     /gene="gltJ"
                     /locus_tag="OQS60_000813"
     CDS             13427..14167
                     /gene="gltJ"
                     /locus_tag="OQS60_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS2386644.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14167..14841
                     /gene="gltK"
                     /locus_tag="OQS60_000814"
     CDS             14167..14841
                     /gene="gltK"
                     /locus_tag="OQS60_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS2386645.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14841..15566
                     /gene="gltL"
                     /locus_tag="OQS60_000815"
     CDS             14841..15566
                     /gene="gltL"
                     /locus_tag="OQS60_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS2386646.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15684..16619
                     /gene="rihA"
                     /locus_tag="OQS60_000816"
     CDS             15684..16619
                     /gene="rihA"
                     /locus_tag="OQS60_000816"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS2386647.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16711..18381
                     /gene="hscC"
                     /locus_tag="OQS60_000817"
     CDS             16711..18381
                     /gene="hscC"
                     /locus_tag="OQS60_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS2386648.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18441..19892)
                     /gene="djlC"
                     /locus_tag="OQS60_000818"
     CDS             complement(18441..19892)
                     /gene="djlC"
                     /locus_tag="OQS60_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS2386649.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19889..20596)
                     /locus_tag="OQS60_000819"
     CDS             complement(19889..20596)
                     /locus_tag="OQS60_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS2386650.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20698..21252
                     /locus_tag="OQS60_000820"
     CDS             20698..21252
                     /locus_tag="OQS60_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS2386651.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21262..22689)
                     /locus_tag="OQS60_000821"
     CDS             complement(21262..22689)
                     /locus_tag="OQS60_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS2386652.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22686..23393)
                     /locus_tag="OQS60_000822"
     CDS             complement(22686..23393)
                     /locus_tag="OQS60_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS2386653.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23557..24534
                     /locus_tag="OQS60_000823"
     CDS             23557..24534
                     /locus_tag="OQS60_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS2386654.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24604..25086)
                     /locus_tag="OQS60_000824"
     CDS             complement(24604..25086)
                     /locus_tag="OQS60_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS2386655.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25321..27903
                     /gene="leuS"
                     /locus_tag="OQS60_000825"
     CDS             25321..27903
                     /gene="leuS"
                     /locus_tag="OQS60_000825"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS2386656.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            27918..28499
                     /gene="lptE"
                     /locus_tag="OQS60_000826"
     CDS             27918..28499
                     /gene="lptE"
                     /locus_tag="OQS60_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS2386657.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28499..29530
                     /gene="holA"
                     /locus_tag="OQS60_000827"
     CDS             28499..29530
                     /gene="holA"
                     /locus_tag="OQS60_000827"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS2386658.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29532..30173
                     /gene="nadD"
                     /locus_tag="OQS60_000828"
     CDS             29532..30173
                     /gene="nadD"
                     /locus_tag="OQS60_000828"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS2386659.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30197..30808
                     /locus_tag="OQS60_000829"
                     /pseudo
     CDS             30197..30808
                     /locus_tag="OQS60_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31068..31385
                     /gene="rsfS"
                     /locus_tag="OQS60_000830"
                     /gene_synonym="rsfA"
     CDS             31068..31385
                     /gene="rsfS"
                     /locus_tag="OQS60_000830"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS2386660.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31389..31856
                     /gene="rlmH"
                     /locus_tag="OQS60_000831"
     CDS             31389..31856
                     /gene="rlmH"
                     /locus_tag="OQS60_000831"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS2386661.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31887..33788
                     /gene="mrdA"
                     /locus_tag="OQS60_000832"
     CDS             31887..33788
                     /gene="mrdA"
                     /locus_tag="OQS60_000832"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS2386662.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33791..34903
                     /gene="mrdB"
                     /locus_tag="OQS60_000833"
                     /gene_synonym="rodA"
     CDS             33791..34903
                     /gene="mrdB"
                     /locus_tag="OQS60_000833"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS2386663.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            34914..36002
                     /gene="rlpA"
                     /locus_tag="OQS60_000834"
     CDS             34914..36002
                     /gene="rlpA"
                     /locus_tag="OQS60_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS2386664.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36141..37352
                     /gene="dacA"
                     /locus_tag="OQS60_000835"
     CDS             36141..37352
                     /gene="dacA"
                     /locus_tag="OQS60_000835"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS2386665.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37462..37725
                     /locus_tag="OQS60_000836"
     CDS             37462..37725
                     /locus_tag="OQS60_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS2386666.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37826..38467
                     /gene="lipB"
                     /locus_tag="OQS60_000837"
     CDS             37826..38467
                     /gene="lipB"
                     /locus_tag="OQS60_000837"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS2386667.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38727..39680
                     /locus_tag="OQS60_000838"
     CDS             38727..39680
                     /locus_tag="OQS60_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS2386668.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39889..40854
                     /gene="lipA"
                     /locus_tag="OQS60_000839"
     CDS             39889..40854
                     /gene="lipA"
                     /locus_tag="OQS60_000839"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS2386669.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10273 a        10418 c        10265 g         9945 t
ORIGIN      
        1 cccccgtaaa atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat
       61 acatcctcac accagaccct ttttcaccaa tcgacaatca ccattacgtt ttttattttt
      121 tcaccgccaa atcgcttgcg cacagtgctg ctttgcggca tttttttaaa caagcaaaca
      181 caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc
      241 gatatcaaaa cagacgcagt tgagcagcgt aagaaagcac tcgagctgtc acgcctgatg
      301 cgtcatcgtg gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgctcac
      361 gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa
      421 acccacgtac tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat
      481 ggcgatcgtt atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag
      541 gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac
      601 agcgaaaaag atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg
      661 gggtatgacg aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt
      721 tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagccagga cggcgaaatc
      781 cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac
      841 aaaaacgagc tgcgtcaggc tctggaagat tccgttaaaa gccatctgat gtctgatgtg
      901 ccttacggtg tgctgctttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag
      961 aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccccagtta
     1021 cactcctttg ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca
     1081 aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc
     1141 atccgcgacg tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca
     1201 ccgatgtatt taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt
     1261 gaaggttctg atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa
     1321 gaactgcatg aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc
     1381 gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa
     1441 ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg
     1501 gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg
     1561 cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg
     1621 gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac
     1681 acgccaacct caaaagaagc gtatctgtac cgggagatct ttgaagaact gttcccgctt
     1741 ccgagcgccg ctgagtgcgt gccgggcggc ccgtccgtcg cgtgttcttc cgctaaagcg
     1801 attgagtggg atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc ggttggcgtt
     1861 caccagtcgg cgtataagta agaaatgcga aacagccccg gtgaaatccg gggctgtttc
     1921 agttattgag gtgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca
     1981 tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt cccaccacca
     2041 ttgttgaatt ccgaatttca tcctgtatga caaaagaatg gctgtagggt ggggaaaaaa
     2101 cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag
     2161 tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa
     2221 tgcgccccgc aacgccgata aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt
     2281 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct
     2341 tttttgcggg agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt
     2401 gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac ggatggggta
     2461 tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac
     2521 cccagccatc ttcttcgagt aagcggttca ccgaccggtt attggggtat cgccaagcgg
     2581 taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatcg
     2641 aagaaacaat ctggctacgt agctcagttg gttagagcac atcactcata atgatggggt
     2701 cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac gttcggcaaa
     2761 ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
     2821 attccgaggt tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt
     2881 tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg
     2941 tcggatgcga cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg
     3001 taaggctaca accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct
     3061 gccgccatta acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg
     3121 gcataaaaca gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg
     3181 taacgcttga ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga
     3241 acatcaatca gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga
     3301 tggatggtat gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca
     3361 tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac
     3421 gggaaatgct tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga
     3481 atacgcgccg gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac
     3541 gcacgcggtc cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac
     3601 tgtacgctaa tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc
     3661 atctgccgca cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt
     3721 tcaccgccct tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag
     3781 cctggcacac gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc
     3841 aggacggtgt tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac
     3901 accacatgcg ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata
     3961 gcctgtactg catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc
     4021 gccgagatcc gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg
     4081 atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt
     4141 cctccgccga caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc
     4201 aatatgcttt aagtttacag gatttttacc cccaagggct gataacgctc atactggtca
     4261 caacggcagc aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc
     4321 aagtcgatga ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat
     4381 tcatcgaaga tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg
     4441 gaagaagcgg atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa
     4501 gtcttccatc agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc
     4561 ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat
     4621 gtcgatatta tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg
     4681 cgcggcgacc gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt
     4741 ctaccggaac cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat
     4801 aaatattgca cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct
     4861 gacgatattc tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg
     4921 ctcggtcaga acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt
     4981 gccgatctgc tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc
     5041 agccatccga tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg
     5101 gtgagcttcc tgcatctgcc agtacaaagc ggttccgatc gcattctgaa cctgatgggc
     5161 cgtactcaca cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg
     5221 gatattcaga tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc
     5281 gagaaaacga tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc
     5341 tctgcacgtc cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag
     5401 aagcaacgtc tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc
     5461 cggatgctcg gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg
     5521 gagctttccg ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg
     5581 atcggtaaat tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa
     5641 gtggtgcgta ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt
     5701 gcccgtaccc gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc
     5761 ccgccgttcc ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg
     5821 cgcctttttc ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc
     5881 ggcatacaga taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag
     5941 cagacaacgc gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg
     6001 aacgccgtct cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc
     6061 cgatttgcgt caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga
     6121 tgcgcggtca gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc
     6181 gagtactgga gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa
     6241 ccaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc
     6301 tcgaccatga cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag
     6361 tggctgcggc agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc
     6421 cggcggtcga agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag
     6481 acccgtatct gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg
     6541 agaaactgat tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta
     6601 cgctgaacga cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga
     6661 agatgttcct gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac
     6721 agatcgacct gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg
     6781 atgtcgaaga gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg
     6841 tggcgcgtat cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg
     6901 cgctggcggc agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga
     6961 tttacaactg gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg
     7021 gctgaatgcg gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga
     7081 taccgccgaa agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt
     7141 gctctccttc ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt
     7201 tatctgccgt caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca
     7261 ctgggcgcat atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga
     7321 tgacgaagca gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga
     7381 ggatccgtac attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg
     7441 ccgttggtga ttaacgacga cgttttgatt aactaacatg agaacccata cgacgccatg
     7501 agcgacgaca attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg
     7561 ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc
     7621 cgtgattccg ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg
     7681 atggacatcg cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc
     7741 ctgaaacgca accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca
     7801 cgtttcccgg tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac
     7861 ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag
     7921 gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag
     7981 cgttaccaca tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt
     8041 gaagacatcc tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat
     8101 atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa
     8161 gacttcaacg aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt
     8221 ctggtgatgc aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt
     8281 taccagttca aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc
     8341 ccggatgact caccccagcc gaagctggat gaataaaacc gaaactggat agataactac
     8401 atggcttttg cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc
     8461 ggtgcctgcg gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg
     8521 ttgatggggc ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc
     8581 ttttgctggg gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg
     8641 acctttggcg gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat
     8701 ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc
     8761 tggctgcgcg tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt
     8821 tgggtactga caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta
     8881 aaagggctgg caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt
     8941 ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg
     9001 ctgtttgccc tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa
     9061 accattcagg tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat
     9121 cagcttctta atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca
     9181 tcgttgatta tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc
     9241 ctcaaagcac tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc
     9301 gacgcgcgtc tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt
     9361 aaaggtgcac catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg
     9421 tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg
     9481 ccgatgtcgt cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt
     9541 gagcttactg cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc
     9601 cgcccggata ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt
     9661 ggtccatggc agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg
     9721 ttgcgcagca ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg
     9781 atgatcccgc agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc
     9841 acaccgtatg cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt
     9901 gccgctgtgt tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat
     9961 gagcaaaacg tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac
    10021 ggttcaggga tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca
    10081 agcgagcgcg tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta
    10141 ccatccgctg aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc
    10201 ccggcattaa tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa
    10261 tattgaccgt ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc
    10321 ataatgctgg cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa
    10381 ataacctcgc cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct
    10441 gcgggaaagt cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct
    10501 gctcccggaa ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt
    10561 ttaccgctcg tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa
    10621 gtcaccagat caatcgctgg cacactatta ctggtgcgat tcatcaccca ggcttttgtg
    10681 ccatcacgcg agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc
    10741 tcccctgtcg ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc
    10801 aacttttcat ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa
    10861 tgtttgactg acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt
    10921 ccgaggtcga aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca
    10981 tcccgactga tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc
    11041 tgttcacgac ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac
    11101 tgctgctttt tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg
    11161 ctttgcctga cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg
    11221 gtcagaagaa gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca
    11281 gataaacgcg cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa
    11341 gcgttatcta ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac
    11401 agaggaaata ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt
    11461 gctatcgctg caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc
    11521 tgctcacttt cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat
    11581 aaccaggtaa tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc
    11641 aggaatgcaa acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc
    11701 aataagcgcc agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata
    11761 agtgcggatt gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca
    11821 atacagagtg caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc
    11881 gaagatgcag acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt
    11941 gtgcagttat catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga
    12001 ctttttcagc ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta
    12061 agttgcttta tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac
    12121 cacaaatcag cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg
    12181 gcgcatctcg caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat
    12241 gttacctaac aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc
    12301 acaacgggta tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt
    12361 aaacctgcca cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac
    12421 gccgccccgg cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc
    12481 ggtcaccgtg aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt
    12541 tactcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg
    12601 gacttgcagg taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac
    12661 ggcactttcg attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg
    12721 gctttctctg atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat
    12781 atcaaagatt ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct
    12841 gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc
    12901 aaagatcaca gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg
    12961 gatgatgccc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc
    13021 gtcggcaagc cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag
    13081 ttcaaaaagc tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa
    13141 tggtttgata aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa
    13201 ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta
    13261 caagaaccag gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata
    13321 cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg
    13381 agggtagcag ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg
    13441 gaactggggt atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat
    13501 ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct
    13561 cgtcggttca tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg
    13621 tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta
    13681 tctggtgatc ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga
    13741 tcccaatatt cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg
    13801 tgtttgcgaa caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc
    13861 gctggcgatg ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta
    13921 tcgcgttatc gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat
    13981 cgcctcgact attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc
    14041 agcccacgcc tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt
    14101 tatcatgctg gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg
    14161 caaataatgt acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac
    14221 gggctggtga tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc
    14281 accatgctgg cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc
    14341 tacgttaacg tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc
    14401 gtgccaggtt ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt
    14461 tcggcgatgg tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct
    14521 ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac
    14581 tggcagtcga tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg
    14641 ctcactcagg gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc
    14701 gatttcttcc gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc
    14761 ctgtttgccg gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac
    14821 ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact
    14881 ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg
    14941 gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc
    15001 agcaaggtga aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa
    15061 agctgcgttc ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga
    15121 ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc
    15181 gtgaaaaagc attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc
    15241 cggcgcagct ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg
    15301 atcctattgc gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg
    15361 aagtgctgga cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc
    15421 acgaaatggg ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa
    15481 ttgtcgaaga ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag
    15541 acttcctcgc gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc
    15601 tcacgcttta tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa
    15661 gaagcacaca acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc
    15721 atgacgacgc tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa
    15781 ttacgtcttc cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc
    15841 tgaccttgct taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc
    15901 gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac
    15961 cggaaccgac gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc
    16021 gcgaaagtgc ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc
    16081 tgctcaatag ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg
    16141 caatggggct tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag
    16201 cggcagaaat tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc
    16261 acaaagcaca aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt
    16321 caaccattgt tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg
    16381 gctttgtcgg tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt
    16441 tgtttaccac tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta
    16501 tgacggttgt tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg
    16561 atgttgatcg tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac
    16621 acacgtcatt gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt
    16681 tatctctttt tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc
    16741 gatctcggca ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt
    16801 ccaaataagt tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat
    16861 attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg
    16921 ttatttaaac gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac
    16981 gcgccagaac tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg
    17041 caacgtccga ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc
    17101 aagcataccc gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa
    17161 cccacagcag ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt
    17221 tttgatctcg gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt
    17281 gaagttcacg cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg
    17341 gtcgatgacg ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg
    17401 gcagccttgt acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt
    17461 cgctggcagt accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat
    17521 ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca
    17581 cgtctgaagc cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg
    17641 ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg
    17701 agcaccattg tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat
    17761 attgaagagg tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc
    17821 cagggcgttt ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg
    17881 cgtgtagaaa cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat
    17941 cagggagaaa accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg
    18001 aagaaaaccg gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg
    18061 cttgaagttg acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc
    18121 ccggtaacat tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa
    18181 atttatccgc gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg
    18241 gcgcgggcgc tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg
    18301 gcgttgcagt caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat
    18361 ttggctattg agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca
    18421 tcggcgcaaa aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct
    18481 atcatcagca ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc
    18541 tcatcatttt tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc
    18601 tcgagtttca ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagcatct
    18661 ccaccttcca gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc
    18721 acaacctgtt ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta
    18781 ccgactctcg atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac
    18841 gattgaccta acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc
    18901 cgggcgctct caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc
    18961 gcttcgataa ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc
    19021 aacaacaaat tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca
    19081 agcaggcttt ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct
    19141 tcgctttctg gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct
    19201 gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg
    19261 aaccacggtc cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa
    19321 cgttccagcg cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga
    19381 aggctaagga aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct
    19441 tcctcatcca ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa
    19501 gaagcacaat ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt
    19561 aattgatcga catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg
    19621 gagggctgaa aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca
    19681 tgttcagcgg tggcatcttt ttcttcatca tcggcttcct cgacaggatt taccgccagt
    19741 cgcagcgcct cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga
    19801 tgacacaacg gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt
    19861 tcaatttcga ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg
    19921 ggcattcagg ctggacagtt aatggttgcc agggaagcgt ggcatagaaa cggtcgcttc
    19981 ccgctgccag ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg
    20041 cctcgcgtag cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg
    20101 aataggcaga aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc
    20161 gcgattgcag ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg
    20221 acagaaaccc cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag
    20281 cggtttgggc aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg
    20341 ctttcctctc ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg
    20401 catgaccatc atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc
    20461 cccaggatga tgagagatcg aattctttta tcccagtata catttttggc gagtcatgcg
    20521 cgggtttgca actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt
    20581 gttcttcctt attcattttt taataatccc ttaagtgcat taactgacta cttttttatt
    20641 attgatgagt ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg
    20701 aataaaaaat caatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc
    20761 caacccaaga aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta
    20821 agatcaccag gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt
    20881 cactcaacat cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca
    20941 ctggattata cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc
    21001 gcacaaagca aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt
    21061 aaagcaattc tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact
    21121 ttgggtttga tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa
    21181 tggttaaaac tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt
    21241 cccgcacatt aacgtcgact gtcacccgaa gagatggagt atttttccta tcaaaccggc
    21301 aaagataaaa atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcgtc
    21361 aacgggcatt ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc
    21421 cgcctgaatt aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc
    21481 aaggcggctc acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg
    21541 tttggcaact aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg
    21601 cggcttacgt cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt
    21661 ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata
    21721 aagtagctgt ttaccctgat cgttaaccag ttcacgagct tcaatcatca tcaccactaa
    21781 cgggtaataa tcgacccgat gctgacggca ccattgcaat attaaagcat ccgcctgggt
    21841 ggagggatac tcgcgccagt agtcacaaag ttctgccagc aagctttcgc cttctccaca
    21901 atagacgcgt tgcgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg
    21961 ccacgaccgg gcgtaatcca gaagttcggg gatatccagg cgacctgcgc tataccagcg
    22021 gagtaaattt ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa
    22081 atacaatgtc agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc
    22141 gatggtgtca ttttgcacgg caacgggcag atgcagaatg gtctggaagt taaaaacatt
    22201 ccccttcgca atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt
    22261 ttcctcctgc aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg
    22321 ggcagtgttc atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga
    22381 gcaataattt aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc
    22441 agaggcaagt aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc
    22501 tggttgccaa acagatttag caggcgactg cgcaatccgg agcgcctcct catacgcctg
    22561 acgaagttgt ttaaaaccct gcggatcggt ttctggatga aaggacagta acagcgccag
    22621 ataagcgcgg cggataatat cgacgtcagt cgtttcctct atttcgagga tcttccagca
    22681 atttttcata ggtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt
    22741 ggttgccagg acaaggcagg gtaatacgca tcatcactgg caaataattg ataaaacata
    22801 tcattgccgg aatcgtcgta ttctttacga gctaagaatt cgcgtaataa aggcagatga
    22861 tcatcttcgg gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc
    22921 cagttactat aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct
    22981 tcttcactca accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa
    23041 tcccatgctt taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca
    23101 taagcctgac cttgctcatt tagttcagca agatagtcac gccattcaca tggcgaataa
    23161 cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat gaccgttatc gatcattcgg
    23221 tgaattgtgc gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa
    23281 aagcatgtac ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt
    23341 atttccatcg gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt
    23401 atccttaaac attcatttaa tgaatgtaaa ctacatttcg agactcagtt atttatactt
    23461 ttctgacgca acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat
    23521 ataatatgtt ttatctacaa ccataaggaa atgataatgg tttttacgtc aagttgctgc
    23581 gataatttat caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa
    23641 tatattgttg ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag
    23701 gccttttact ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta
    23761 gggcaaaagt ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg
    23821 ctgaaaaaag ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggacactg
    23881 gatcgtggag aagcccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag
    23941 agcggaatgt cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc
    24001 gcaaaagact atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt
    24061 gacgcgcaaa acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacaag
    24121 acactcgccg cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt
    24181 caaattgcat gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg
    24241 tactggtatc tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat
    24301 atgtataaac acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg
    24361 aaagccgctg aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac
    24421 ggagaaggaa gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca
    24481 tccggaacca gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa
    24541 aaacaagggc cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga
    24601 ctattacggc tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt
    24661 cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc
    24721 cagcccgacc acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc
    24781 gcgccattca agctgcgttt tatcggtgat atctgccagc tcctgccata agctttcttt
    24841 aatcacccgc ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc
    24901 gaactcttcc aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa
    24961 ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc
    25021 gccattgcgc aggcgttcgc tcagtgacgc gaccagttca cggtaatatt gagcaacctt
    25081 gttcatcgtt tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct
    25141 tgctttgcta atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt
    25201 tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat
    25261 ctgaaaaacc acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc
    25321 atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag
    25381 aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg
    25441 cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac
    25501 gtgatcgccc gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac
    25561 gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg
    25621 acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac
    25681 tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc
    25741 accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg
    25801 aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat
    25861 accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac
    25921 gagctgctca acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag
    25981 cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac
    26041 aacatgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg
    26101 gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc
    26161 tttattgacg aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa
    26221 aaaggcgtcg atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt
    26281 tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt
    26341 cacgaccagc gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc
    26401 ctggctgctg acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg
    26461 ctgttcaact ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc
    26521 gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg
    26581 ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt
    26641 accgtaatgc cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg
    26701 gacggcatta ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt
    26761 atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat
    26821 gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac
    26881 tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac
    26941 ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg
    27001 aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac
    27061 ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt
    27121 atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg
    27181 tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac
    27241 accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa
    27301 tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac
    27361 acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg
    27421 ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag
    27481 accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca
    27541 accgatggcg agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg
    27601 cttaacccgt tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc
    27661 gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg
    27721 ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct ggtggacgca
    27781 acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat
    27841 ggcgttaccg tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc
    27901 taagcgcggg aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa
    27961 tcaccgccgg gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg
    28021 tcatgatcct ggactcaggc gatccgaacg ggccattaag ccgtgcagtg cgtaaccagt
    28081 tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc
    28141 tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa
    28201 cagcagagta tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata
    28261 tctacccgat tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag
    28321 cgaaagataa cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc
    28381 tgattcgtaa gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt
    28441 cgaccacaac ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat
    28501 gattcggttg tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta
    28561 tcttttactt ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt
    28621 agctgcggca caaggattcg aagaacacca cactttttcc attgatccca acactgactg
    28681 gaatgcgatc ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt
    28741 gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac
    28801 cggacttctg catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca
    28861 agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac
    28921 accggagcag gctcagcttc cccgctgggt tactgcgcgc gcaaaacagc tcaacttaga
    28981 actggatgac gcggcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct
    29041 ggctcaggca ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg
    29101 cgttgaacag gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt
    29161 gttgatggga aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag
    29221 cgaaccggtt attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa
    29281 acgccagtct gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa
    29341 ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc
    29401 cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg
    29461 ggcagagctg aaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat
    29521 cgacggttga tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact
    29581 atggtcatct aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa
    29641 tcatccctaa taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta
    29701 aacacatgct tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc
    29761 taaagcgcaa tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag
    29821 gaccggacgt gccgctggcg tttattattg gtcaggattc actgctgacc tttccgacct
    29881 ggtacgaata cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt
    29941 acccacttga aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata
    30001 acccggaaga tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt
    30061 ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt
    30121 tattgccgga accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt
    30181 cgttacggag ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat
    30241 cgatggtctt tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc
    30301 gcaaaatctg catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact
    30361 ggaacgggca cagcataccg cacgactggt tctcagtgac cgccagctcc ccgtgcaaat
    30421 catccctgaa ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct
    30481 catgcaagaa gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc
    30541 cacgaacggt gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct
    30601 tagtgaattt cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct
    30661 gttaatcgcc cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacta
    30721 ggggtgttgg agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag
    30781 ccgtgccatc ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg
    30841 accattgaca gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac
    30901 gactttgtag aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga
    30961 tagcccactt tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt
    31021 atactgacag accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag
    31081 cactccagga ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct
    31141 tagacgttca aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca
    31201 gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt
    31261 taccgctcgg cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg
    31321 tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga
    31381 gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa
    31441 ccggttttac cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa
    31501 ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg
    31561 agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc
    31621 cctgggatac gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg
    31681 tcagtctact gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc
    31741 agagctggtc gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag
    31801 agagtctgta ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag
    31861 ggagctttga gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg
    31921 cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga
    31981 ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga
    32041 ctcgctctaa tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct
    32101 acgaccgtaa tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc
    32161 cagagaaagt cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga
    32221 ccgatgacga tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta
    32281 ttccggtgaa aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc
    32341 gttttccggg tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt
    32401 tgacccacgt catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata
    32461 atgacggcaa actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc
    32521 gttactatga agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc
    32581 gtgggcgtgt tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc
    32641 tgacgctgga tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag
    32701 ctgtggttgt caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt
    32761 atgacccaaa cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg
    32821 ccccgaatac accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag
    32881 ttaaacccta tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc
    32941 tgtttgaccc aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa
    33001 aatggggcca cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct
    33061 tctatcaggt ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg
    33121 gttatggtca ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc
    33181 gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg
    33241 gtatcggtca gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc
    33301 tgattaatga cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca
    33361 aacaggtgcc atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact
    33421 gggagctggc gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata
    33481 aatactttgc cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg
    33541 gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca
    33601 aactgatgac cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg
    33661 agaacggtgg tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta
    33721 tgctgggtga taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg
    33781 accattaatc atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga
    33841 tcccacaatg ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag
    33901 cgccagcggt caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct
    33961 agtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta
    34021 tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa
    34081 aggtgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa
    34141 aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt
    34201 gaagaacact ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca
    34261 gcctgacctg ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc
    34321 tggccttagc tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat
    34381 tctgtggttc ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctagaccc
    34441 ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc
    34501 cggcggatta cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc
    34561 cgaacgccat accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat
    34621 tctgattctg ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag
    34681 agcgcaaacc acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta
    34741 tgtcttcgta aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc
    34801 actggtcagt tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat
    34861 gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta
    34921 agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg
    34981 gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa
    35041 ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc
    35101 gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg
    35161 cggcaatcta tgatgccgaa cccggcagta acctgacggc ctctggcgaa gctttcgatc
    35221 cgacacagct gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta
    35281 acctggctaa cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg
    35341 acagcgttat ttcactttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca
    35401 aagttcgtat cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg
    35461 cttgtaccac agtcgccaaa cagacttacg ccctgccagc acctcccgat ttaagcggtg
    35521 gcgcgggaac aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga
    35581 cgctaaaaag cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc
    35641 caacgacctt agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg
    35701 ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg
    35761 cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc
    35821 gtgcgcaaca gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc
    35881 aaaatggcgc ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta
    35941 ccttgcagca acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt
    36001 agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg
    36061 aaagtcagat gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca
    36121 cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg
    36181 cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata
    36241 tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact
    36301 ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc
    36361 tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag
    36421 aaactgattt agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag
    36481 gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg
    36541 gtattaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta
    36601 gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata
    36661 cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata
    36721 tggcgctgat cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag
    36781 aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata
    36841 acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg
    36901 ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt
    36961 ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa
    37021 ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt
    37081 ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca
    37141 tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa
    37201 agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc
    37261 cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca
    37321 ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc
    37381 gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa
    37441 ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact
    37501 ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa
    37561 gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc
    37621 aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg
    37681 tatgaagaac taggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta
    37741 cccggcccct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt
    37801 actcattctc cacggagatg cagttttgta tcaggataaa attcttgtcc gccagctcgg
    37861 tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga
    37921 tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc
    37981 aggaaaagcg gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg
    38041 tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa
    38101 acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac
    38161 cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg
    38221 ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct
    38281 ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc
    38341 cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc
    38401 accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac
    38461 cgcttaattg catacctcaa tggcccaatt tacactgggt cattactcat aatacctgct
    38521 aatgctttac atctccttcg cgtttgtata ctttctttat cacttgcaga aatgcggtgc
    38581 tacgccttgc tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga
    38641 gcaaaagcgt tccgttccaa ttagacataa gattattgta atttttttaa atattatttt
    38701 tccatgaata aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa
    38761 gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta
    38821 accttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag
    38881 tgcttaatct gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc
    38941 ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc
    39001 tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa
    39061 gctatgataa gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc
    39121 ttcctgtcat ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc
    39181 ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca
    39241 tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa
    39301 tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg
    39361 ctgcgcatgt actcctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc
    39421 aagagatgtt tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg
    39481 cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta
    39541 gtcgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt
    39601 tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg gtaatagatg
    39661 ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa
    39721 aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc
    39781 gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat
    39841 tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc
    39901 attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt
    39961 aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag
    40021 cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg
    40081 cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca
    40141 gcaacgttta tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc
    40201 cacggtcgcc cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc
    40261 gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc
    40321 ggtgcccagc actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa
    40381 attgaaactc tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact
    40441 gcaacgccac cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag
    40501 gtacggcctg gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg
    40561 catccggaaa tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa
    40621 attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa
    40681 tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc
    40741 gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt
    40801 gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt
    40861 aataattact tacatctgtc tgataaaaaa cccgcttttt g
//
LOCUS       DAJVYM010000016        40849 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVYM010000016 DAJVYM010000000
VERSION     DAJVYM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.016"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            67..747
                     /locus_tag="OQS60_000840"
                     /pseudo
     CDS             67..747
                     /locus_tag="OQS60_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
     gene            749..985
                     /locus_tag="OQS60_000841"
     CDS             749..985
                     /locus_tag="OQS60_000841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386670.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1158..1833)
                     /locus_tag="OQS60_000842"
                     /pseudo
     CDS             complement(1158..1833)
                     /locus_tag="OQS60_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1978..2586)
                     /locus_tag="OQS60_000843"
     CDS             complement(1978..2586)
                     /locus_tag="OQS60_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS2386671.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2770..3087)
                     /gene="metJ"
                     /locus_tag="OQS60_000844"
     CDS             complement(2770..3087)
                     /gene="metJ"
                     /locus_tag="OQS60_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS2386672.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3364..4524
                     /gene="metB"
                     /locus_tag="OQS60_000845"
     CDS             3364..4524
                     /gene="metB"
                     /locus_tag="OQS60_000845"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS2386673.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4527..6959
                     /gene="metL"
                     /locus_tag="OQS60_000846"
     CDS             4527..6959
                     /gene="metL"
                     /locus_tag="OQS60_000846"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS2386674.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7308..8198
                     /gene="metF"
                     /locus_tag="OQS60_000847"
     CDS             7308..8198
                     /gene="metF"
                     /locus_tag="OQS60_000847"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS2386675.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8527..10707
                     /gene="katG"
                     /locus_tag="OQS60_000848"
     CDS             8527..10707
                     /gene="katG"
                     /locus_tag="OQS60_000848"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS2386676.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10801..11706
                     /gene="yijE"
                     /locus_tag="OQS60_000849"
     CDS             10801..11706
                     /gene="yijE"
                     /locus_tag="OQS60_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS2386677.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11733..12350)
                     /locus_tag="OQS60_000850"
     CDS             complement(11733..12350)
                     /locus_tag="OQS60_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS2386678.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12624..13727)
                     /gene="gldA"
                     /locus_tag="OQS60_000851"
     CDS             complement(12624..13727)
                     /gene="gldA"
                     /locus_tag="OQS60_000851"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS2386679.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13738..14400)
                     /gene="fsa"
                     /locus_tag="OQS60_000852"
     CDS             complement(13738..14400)
                     /gene="fsa"
                     /locus_tag="OQS60_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS2386680.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14412..16913)
                     /gene="ptsP"
                     /locus_tag="OQS60_000853"
     CDS             complement(14412..16913)
                     /gene="ptsP"
                     /locus_tag="OQS60_000853"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS2386681.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17222..18301
                     /locus_tag="OQS60_000854"
     CDS             17222..18301
                     /locus_tag="OQS60_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS2386682.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18316..18636
                     /locus_tag="OQS60_000855"
                     /pseudo
     CDS             18316..18636
                     /locus_tag="OQS60_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18687..20983
                     /locus_tag="OQS60_000856"
                     /pseudo
     CDS             18687..20983
                     /locus_tag="OQS60_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20949..21827
                     /locus_tag="OQS60_000857"
     CDS             20949..21827
                     /locus_tag="OQS60_000857"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS2386683.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21829..22170
                     /locus_tag="OQS60_000858"
     CDS             21829..22170
                     /locus_tag="OQS60_000858"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS2386684.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22157..23008)
                     /locus_tag="OQS60_000859"
     CDS             complement(22157..23008)
                     /locus_tag="OQS60_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2386685.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23223..24956)
                     /locus_tag="OQS60_000860"
     CDS             complement(23223..24956)
                     /locus_tag="OQS60_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS2386686.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25139..27790)
                     /gene="ppc"
                     /locus_tag="OQS60_000861"
     CDS             complement(25139..27790)
                     /gene="ppc"
                     /locus_tag="OQS60_000861"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS2386687.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28142..29293)
                     /gene="argE"
                     /locus_tag="OQS60_000862"
     CDS             complement(28142..29293)
                     /gene="argE"
                     /locus_tag="OQS60_000862"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS2386688.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29447..30451
                     /gene="argC"
                     /locus_tag="OQS60_000863"
     CDS             29447..30451
                     /gene="argC"
                     /locus_tag="OQS60_000863"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS2386689.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30459..31235
                     /gene="argB"
                     /locus_tag="OQS60_000864"
     CDS             30459..31235
                     /gene="argB"
                     /locus_tag="OQS60_000864"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS2386690.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31296..32669
                     /gene="argH"
                     /locus_tag="OQS60_000865"
     CDS             31296..32669
                     /gene="argH"
                     /locus_tag="OQS60_000865"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS2386691.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32936..33853
                     /gene="oxyR"
                     /locus_tag="OQS60_000866"
     CDS             32936..33853
                     /gene="oxyR"
                     /locus_tag="OQS60_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS2386692.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33836..35236)
                     /gene="sthA"
                     /locus_tag="OQS60_000867"
     CDS             complement(33836..35236)
                     /gene="sthA"
                     /locus_tag="OQS60_000867"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS2386693.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35557..36207
                     /gene="fabR"
                     /locus_tag="OQS60_000868"
     CDS             35557..36207
                     /gene="fabR"
                     /locus_tag="OQS60_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS2386694.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36207..36566
                     /locus_tag="OQS60_000869"
     CDS             36207..36566
                     /locus_tag="OQS60_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS2386695.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36606..37706)
                     /gene="trmA"
                     /locus_tag="OQS60_000870"
     CDS             complement(36606..37706)
                     /gene="trmA"
                     /locus_tag="OQS60_000870"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS2386696.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37820..38010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38075..39919
                     /gene="btuB"
                     /locus_tag="OQS60_000871"
     CDS             38075..39919
                     /gene="btuB"
                     /locus_tag="OQS60_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS2386697.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39864..40721
                     /gene="murI"
                     /locus_tag="OQS60_000872"
     CDS             39864..40721
                     /gene="murI"
                     /locus_tag="OQS60_000872"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS2386698.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9434 a        10176 c        11220 g        10019 t
ORIGIN      
        1 ttttactata accaccacag ccagttaacg tcagccaccg ggcctgacgg gctggaaata
       61 cgccgggaat atgatgaatg gggcaacctg ctgaatgaag agaacccgca tcagctgcag
      121 cagcttattc gtttgccggg gcagcagtat gatgaggagt ccggcctgta ttataaccgc
      181 caccgctatt atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag
      241 gggggatgga acctgtatac atatccgctg agcccggtga atagcatgga tccattagga
      301 ttatatgaat ttaaatcaaa aaatattgat gatattggaa tatttgcatt ggcaatgtgt
      361 aatggagaat caattaacga gaataaagaa tatggtggac taatatgtaa gaagcaaggt
      421 gaatatttcc ccatgaatcc gataagttca aatgataatg atagtgtaga cttgcgaaat
      481 ataaaatgcc ctgaaggttc agagagagta ggcgattatc acactcacgg tttttactct
      541 gacgataaag gaaataaagt aacaaaagaa aatgatgttt atgatagtct aaatttttca
      601 agcaaagatt taacgaattc ttatatgaat ggaatgggaa aaaaagaata cagtagttac
      661 ttgggaacac caaataacac ctatctaaaa tataatccca aagctaaagg gaatggagtt
      721 acaattatca ggcaagggag taattaaaat gaaggcaata caatgttgtt atttccttgc
      781 tttagtctcc agtcatttaa tggctggagt tatgggtgat gataatatat ttttaagtaa
      841 ttcggattca gctgctttat attgtgtggc taaaatattt ccagatggag aaaatatttt
      901 aatatataaa gtaaatataa ttaatgctag ttcaaaaaaa gatgtttttt ttatgaaagc
      961 ccaacatcaa cccacaatga cataatgggg ggcgggggaa tgtcatatgc ctatgaatat
     1021 gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg
     1081 gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa
     1141 ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa
     1201 cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg
     1261 ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct
     1321 ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg
     1381 cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac
     1441 cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc
     1501 tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg
     1561 ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag
     1621 cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt
     1681 aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg
     1741 gccgcggaat cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct gaacggactg
     1801 gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata
     1861 tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt
     1921 cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta
     1981 ccacgaaaac agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc
     2041 ttgtggtgaa actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt
     2101 agcctggacc agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg
     2161 cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc
     2221 gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag
     2281 tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt
     2341 ttcaccatgg gcgatccact gcttgagcgg ggggtatttc accgggccga ccgcttcaaa
     2401 aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt
     2461 cgccagttgg atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt
     2521 ttgtggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt
     2581 tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt
     2641 tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat
     2701 tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc
     2761 ttttttgctt tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt
     2821 tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg
     2881 ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg
     2941 caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg
     3001 aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct
     3061 gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag
     3121 accagaccac acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc
     3181 gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct
     3241 atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc
     3301 attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat
     3361 cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag
     3421 tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat
     3481 gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt
     3541 gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg
     3601 attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac
     3661 tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg
     3721 ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa
     3781 ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc
     3841 tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg
     3901 gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat
     3961 ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc
     4021 actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat
     4081 ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg
     4141 caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg
     4201 ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg
     4261 ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg
     4321 ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc
     4381 atgacacatg caggcatggc accagaagcg cgtgctgccg ccgggatctc cgagacgctg
     4441 ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc
     4501 ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc
     4561 gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg
     4621 cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta
     4681 gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc
     4741 atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg
     4801 ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc
     4861 tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat
     4921 ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg
     4981 atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt
     5041 cttacccgtt gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg
     5101 ggtttatcag ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact
     5161 attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg
     5221 tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc
     5281 tgcgtctgga tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta
     5341 ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg
     5401 atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca
     5461 ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac
     5521 tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg
     5581 gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg
     5641 cgctgaaaat cctcgacgaa gcggggttac ctggcgaact gcgcctgcgt caggggctgg
     5701 cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct
     5761 ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc
     5821 tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg
     5881 tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc
     5941 gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat
     6001 ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg
     6061 ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt
     6121 tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc
     6181 agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca
     6241 acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa
     6301 aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca
     6361 ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg
     6421 gcacgctctc ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg
     6481 atcaggcgtg gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag
     6541 acgtgatgcg caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc
     6601 aggtacgcgt ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct
     6661 ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa
     6721 tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg
     6781 tggaagcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg
     6841 ccatcgaaag ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc
     6901 gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat
     6961 ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata
     7021 tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca
     7081 ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt
     7141 ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt
     7201 tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag
     7261 atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc
     7321 acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg
     7381 tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca
     7441 tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg
     7501 gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag
     7561 cggcaccgca tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg
     7621 actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa
     7681 gtggtaagcc agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt
     7741 tcgatatctc cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg
     7801 atttgcttaa tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct
     7861 tcttcgatgt cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg
     7921 tggaaattat tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg
     7981 atatgaccaa cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg
     8041 atgccgaaac ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa
     8101 gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg
     8161 cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat
     8221 cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga
     8281 aaataaggtt gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc
     8341 ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat
     8401 ttgacctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
     8461 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
     8521 acattgatga gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc
     8581 catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca
     8641 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
     8701 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
     8761 aaagccctgc tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt
     8821 ctgtttattc gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc
     8881 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
     8941 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc
     9001 tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc
     9061 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
     9121 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
     9181 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg
     9241 ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa
     9301 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca
     9361 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     9421 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg
     9481 acccagacgc cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg
     9541 gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt
     9601 atcccggatc cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     9661 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     9721 gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg
     9781 aaatctcgct acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg
     9841 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     9901 gattctggtc tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc
     9961 cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc
    10021 gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa
    10081 gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag
    10141 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
    10201 gcgcgtcagg atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt
    10261 ttccgtaact atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa
    10321 gcccagcaac tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta
    10381 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
    10441 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
    10501 tcgaaagagc tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc
    10561 cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc
    10621 agcagcgatg ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg
    10681 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag
    10741 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
    10801 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
    10861 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
    10921 tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
    10981 ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc
    11041 tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg
    11101 atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa
    11161 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
    11221 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
    11281 ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc
    11341 gtggatttat tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg
    11401 gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg
    11461 gcctacagtg cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa
    11521 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
    11581 ttttcctggt ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg
    11641 attgtgatgg cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg
    11701 atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc
    11761 accatgcgcc agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc
    11821 accagcggcg taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg
    11881 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg
    11941 gggcgtgttt tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg
    12001 atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc
    12061 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
    12121 acatcggaac ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata
    12181 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca
    12241 aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
    12301 tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc
    12361 cgtgaaaata agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat
    12421 gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag
    12481 tgccggatgc ggcgtgaacg ccttatctgt cctaaaaaaa aagaaaattc aatatattgc
    12541 agagattgcg taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg
    12601 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
    12661 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
    12721 atggtttcac attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct
    12781 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
    12841 acggtttcga tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat
    12901 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
    12961 actgcgtgcg ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc
    13021 gcttcaatca cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct
    13081 ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg
    13141 gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac
    13201 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg
    13261 attttggtgt cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac
    13321 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
    13381 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg
    13441 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
    13501 agacggtcga tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt
    13561 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
    13621 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
    13681 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
    13741 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
    13801 taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca
    13861 tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat
    13921 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
    13981 tccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
    14041 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
    14101 tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc
    14161 cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat
    14221 ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc
    14281 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
    14341 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
    14401 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
    14461 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
    14521 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat
    14581 tgcgcttcat catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat
    14641 tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag
    14701 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct
    14761 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt
    14821 tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc
    14881 gccagcaggt gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg
    14941 cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg
    15001 ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct
    15061 cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa
    15121 tcgagcgccc acaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta
    15181 tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
    15241 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
    15301 agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc
    15361 agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc
    15421 aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg
    15481 tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg
    15541 tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
    15601 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
    15661 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
    15721 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
    15781 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc
    15841 tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc
    15901 gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata
    15961 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
    16021 gtgccgccac ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg
    16081 ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg
    16141 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
    16201 gcacgttctt gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca
    16261 ctggcgacaa tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga
    16321 cgtaaagatg tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg
    16381 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
    16441 gtggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
    16501 aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg
    16561 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
    16621 acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
    16681 tggtgcgctt cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc
    16741 gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg
    16801 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
    16861 cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
    16921 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
    16981 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
    17041 acgccgtttt gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt
    17101 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
    17161 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
    17221 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
    17281 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
    17341 tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat
    17401 cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat
    17461 tgcagaacgt tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg
    17521 tgcgggcttc tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
    17581 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
    17641 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
    17701 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
    17761 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
    17821 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc
    17881 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
    17941 tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt
    18001 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
    18061 ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
    18121 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
    18181 ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa
    18241 aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta
    18301 attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgcc
    18361 catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg
    18421 aaagtagaaa cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg
    18481 agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
    18541 ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg
    18601 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
    18661 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
    18721 tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
    18781 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
    18841 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
    18901 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
    18961 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
    19021 gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
    19081 tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata
    19141 aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc
    19201 tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
    19261 cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa
    19321 cgatggcggc aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga
    19381 tctctcgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
    19441 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
    19501 tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt
    19561 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
    19621 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg
    19681 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
    19741 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
    19801 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
    19861 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
    19921 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
    19981 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
    20041 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
    20101 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
    20161 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
    20221 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
    20281 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
    20341 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
    20401 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
    20461 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt
    20521 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    20581 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    20641 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    20701 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc
    20761 gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat
    20821 tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta
    20881 tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga
    20941 gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc
    21001 gcgatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca
    21061 ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg
    21121 agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga
    21181 aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata
    21241 tcagccttga cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg
    21301 gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt
    21361 ttttacagcg actgcggctg tggggtgtgt catgcgccat tgaaactgcc ggagacgcgc
    21421 cagcaagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa
    21481 ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga
    21541 atctgcgttt gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg
    21601 gtttcacgct cagcagggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa
    21661 tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg
    21721 ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc
    21781 gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg
    21841 gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg
    21901 gaaaagttgt gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgctggga
    21961 acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg
    22021 gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc
    22081 tacgcattcc tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg
    22141 ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact
    22201 gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt
    22261 tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca
    22321 ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc
    22381 ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag
    22441 attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata
    22501 gtgcctggct gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa
    22561 gcaggctggc tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat
    22621 tgagcgtttg tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg
    22681 ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc
    22741 caaacagcac gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag
    22801 cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact
    22861 caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg
    22921 ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg
    22981 agagcagata gctgacgccg tgatacatgt tattccctta ggtatgtaga cgggaaaaca
    23041 gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta
    23101 aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagc ctaccgccgc
    23161 gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg cctggctgat
    23221 aattactgat tacctacctg gtcgccatac ggcagagtgt cgtaatcgat cagcgcgttt
    23281 ttcttgtacg ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa
    23341 cgggttggat cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt
    23401 tccgccaggc tgtatttacg atcgacatcc tgtgagaaat cacggggatg agtcgcttgc
    23461 catttttccg acgtccacag caggaacgga atggtgtaca tgtggcgcgt cgggttgtct
    23521 tcgttacgcc cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag
    23581 aagtaaacca ggaatccgtt cgggtctgcc gctttaaagt ctttaatcag gctggcaacc
    23641 acatgatcgt tatacaggtt agcgttgtca taatcgttat atgattccag ctcttctgcg
    23701 cttaatcctg gcggaacatg atcggtattg ccatcaaact tgccctgatt ttccgggtag
    23761 cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca
    23821 gggtcattca gcacatcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc
    23881 tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa taccgtcagc
    23941 atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc
    24001 tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg
    24061 gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt aactacgtta
    24121 ttaaacacgg tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca
    24181 cgcggataac cgtacagact catgcgtccg cgctgggtcg actcgccaat caccagcact
    24241 aaagtgcgcg gttcgttacc cgattcatct ttgaaattag ccagtggcgg caaggcatta
    24301 ttttcattca gtaactttgt tagcgagttt agttgctgac gatactgata atagccggtc
    24361 aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa cgttttctca
    24421 aacggcttgt ttttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc
    24481 agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc
    24541 cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa
    24601 tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg
    24661 ctctgcgaga actcctgccc gtagatgacg tagtagcata gcgccgccag agaggccgcc
    24721 catagcacca cgccgattac tgcggcaata attttaatcc gcttcggaaa gaggaatacc
    24781 gggatcaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt gccactataa
    24841 ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc
    24901 caacccaggg ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta
    24961 agaacctgtc ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa
    25021 ggtatattca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga
    25081 acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagaa gaggaagatt
    25141 agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac gcttgttcga
    25201 cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg
    25261 cctgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc
    25321 acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact ttgatgtctt
    25381 cttcttgcag gttgcgtaac tctttaccta acggccacag tgctttgtct accagacgtt
    25441 gatcatagta ttccgccaac cacaggtctg ctttggcgaa gaccatctcc agcatgccga
    25501 gacgcgtcga gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttgccat
    25561 cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc atcaggcggt
    25621 tttgcgtcca ggcaaaaatc cacggtatgg cgcgcagcga ctcgacgccg ccggttgggc
    25681 gacgtttcgc cggacgtgaa cccaacggca gtttgcccaa ttcttgttcc ggcgtagcgg
    25741 agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg
    25801 agatgactga cagttcatcc ataatgcgac gccagctctc tttcggctca ggtggtggca
    25861 gcaggttggc ttccagaatc gccccggtgt aaagcgacag gctgctgacg gtgatttctg
    25921 gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg ccacctttca
    25981 ggcttcctgg cggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg
    26041 aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag gttttgatta
    26101 atgcatcctg tgcctgatat tgcgcccagg aagctgccat cacgcccgca tcttttgctg
    26161 agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga
    26221 tattgagcag ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca
    26281 gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca
    26341 gtacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg
    26401 cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt
    26461 tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg
    26521 cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt
    26581 cggtatggcg ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt
    26641 tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct
    26701 gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcagcc
    26761 cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca
    26821 tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc
    26881 caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa accagaacct
    26941 gaatatcttt caggaacaga tcggtggctt tccagcgact gagtagcagg acgtggcggg
    27001 tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaagtaaaac
    27061 ggaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt tgttcgttca
    27121 gttcgcgcaa gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc
    27181 atttggcttc atctaccggg cttggacgca gcttacggat ttcatcagta tgccatgact
    27241 gggcgattaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca gcgatatctt
    27301 tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac
    27361 gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt
    27421 ttttgatggt gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga
    27481 tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg
    27541 tgttggccag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg
    27601 acaaattttg taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag
    27661 atttcgacaa cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct
    27721 tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt
    27781 gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg
    27841 tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa
    27901 aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta
    27961 cgccattaaa ttcacgacgc tttaaataag cgtaacttat ggaaatgtta aaaaatcgcc
    28021 atacataaca ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattatt
    28081 gcggcaccgg agtttggcaa cggtgccgga tgcggcgcga gcgccttatc cggcctacgt
    28141 tttaatgcca gcaaaaatgg tgaattacct ggataatcaa ctcacgggtg ggtttaataa
    28201 accgcgtctc cagatattca tcaggctgat gagcttgatt gatggaaccc ggaccgagca
    28261 ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg
    28321 tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat
    28381 agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca
    28441 ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg ctaggcagcg
    28501 gacgaatatc catatgcaac tcacagcaag cgcaaatacg gttagaagcg tcgccaccgt
    28561 gaatatgccc gaggttgagc gtagggtatg gcacggtaaa tgcttcgtag tgataacgtt
    28621 ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt agttcgatag
    28681 cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata cggatggcgt
    28741 tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg ccaatgatgg
    28801 cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt
    28861 cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg tcgacatcgc
    28921 gtagcgtatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa
    28981 gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg tcatcaaatg
    29041 gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg atacttgcca
    29101 gcatattgaa tttgttgcga gttcctggca caggctgcac ttccacattg aagcctaaat
    29161 ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt
    29221 ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta
    29281 atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt
    29341 tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt
    29401 tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct
    29461 gattgtgggt gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc
    29521 gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat
    29581 ctccgatttg catccgcagc taaaaggcat cgttgagctg ccgttgcagc cgatgtcgga
    29641 tatcagcgag tttagcccag gggtggacgt agtgtttctc gccaccgccc atgaagttag
    29701 ccacgattta gcgccgcagt ttcttgaagc gggctgcgtg gtgttcgacc tttccggtgc
    29761 gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc atcaataccc
    29821 ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga
    29881 agcgaatttg attgcggtgc caggctgtta tccgacggcg gcacagctgg cgctgaaacc
    29941 gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg
    30001 cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca
    30061 accgtatggc gtctttactc atcgccatca accagagatc gccacacacc tcggtgctga
    30121 cgttatcttc accccacatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg
    30181 ccgcctgaaa tcgggtgtga cccaggcgca agtcgcgcaa gtgttacagc aggcgtatgc
    30241 ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg
    30301 gctgccattt tgcgatatcg ggtttgccgt tcagggcgag catctgatta ttgtggcgac
    30361 cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca atattcgttt
    30421 cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca
    30481 aactgggcgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcacactgg
    30541 tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg
    30601 tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaacggc ctgcgggtga
    30661 cgcctgctga tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc
    30721 tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg
    30781 acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg gcgcagccag
    30841 gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg gtcagctcca
    30901 ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcaacggcgc
    30961 tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagcggc attctcgacg
    31021 gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg
    31081 gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgcg gcccgcacgc
    31141 tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg gcactgttta
    31201 acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt
    31261 tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta
    31321 cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc
    31381 tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc acggtaggcg
    31441 tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gagcgtgctg ctggaagatg
    31501 ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg
    31561 aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc gggcgtagtc
    31621 gtaacgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt agcgagttac
    31681 tgacggctaa ccgacagctg caatcggcgc tggtggaaac cgcacaaaac aatcaggacg
    31741 cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc gcgcactggt
    31801 gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat gcgcttaagc
    31861 gtctggatgt cagcccatta ggctgtggcg cgctggcggg aacggcctat gaaatcgacc
    31921 gtgaacagtt agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctggacagcg
    31981 tttctgaccg tgaccatgtg ttggaactgc tgtcggctgc cgctatcggc atggtgcatc
    32041 tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc
    32101 tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc
    32161 tggagctgat tcgcggtaaa tgcggtcggg tgcagggtgc gttaaccggc atgatgatga
    32221 cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt
    32281 tcgacgcgct cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca
    32341 ttcaggtgaa acgtccacgt tgccaggaag cggctcagca gggttacgcc aacgccaccg
    32401 aactggcgga ttacctggtg gcgaaaggcg tacctttccg cgaggcgcac catatcgtgg
    32461 gtgaagcggt ggtggaagcc attcgtcagg gcaaaccgct ggaagatctg ccgctcgacg
    32521 agttgcagaa attcagcccg gtgattggcg aagatgtcta tccgattttg tcgctgcaat
    32581 cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc gcagcaggtg gcccaagcga
    32641 ttgcttttgc gcaggcgcgg ttggggtaag aacatttata tgtacaaatt tgagcctggc
    32701 ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc aaaagttcac
    32761 gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt caagggttaa
    32821 aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat agggataatc
    32881 gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat ggataatgaa
    32941 tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc ggcgtgcggc
    33001 agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc tggaagatga
    33061 gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat
    33121 gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc
    33181 aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc ccacggttgg
    33241 accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa agctggaaat
    33301 gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagtg gcaaactcga
    33361 ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc cgttgtttga
    33421 tgagccaatg ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtacc
    33481 gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact gtttgcgcga
    33541 tcaggcaatg ggtttctgtt ttgaagccgg ggcggatgaa gatacacact tccgcgcgac
    33601 cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt tactgccagc
    33661 gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt gcattaagcc
    33721 ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc gcagccgcta
    33781 tgagcagctg gcagaggcca tccgagcaag aatggatggc tatttcgata aagtgttaaa
    33841 acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatg gtcgggtagt
    33901 taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt tccataatcg
    33961 cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaata cccagaatct
    34021 cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg acgatttgtg
    34081 cgcgtgccag atgtttaaac tgggcgcggc ccacttcata tggcactttc atcgcggtca
    34141 gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg ccggtaggga
    34201 tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc
    34261 cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc accgcgtaaa
    34321 cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt
    34381 ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg ttggcataga
    34441 gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca ccgtcgtcac
    34501 agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg ttccagaagt
    34561 gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt
    34621 tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca cagccgatca
    34681 ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctaaga attgagtcgc
    34741 tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga cgagagccgc
    34801 aggcaataac aaatttttca gcggttagtg tttcgacgct gccgtccggg caatccagcg
    34861 ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag tgattacgtt
    34921 cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca tggttaagga
    34981 tatcggcaaa agaagagcgg agcagtcgcg aatggtcgct gtatagtggg ttttgattga
    35041 attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg ccccagtggg
    35101 tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcaca ccttgcttaa
    35161 ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg gcatcgtaat
    35221 cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa cagaatggta
    35281 acattctatc gcgggtaagc caattgatcc ccgtcattta tctggctata tcctgagcgg
    35341 cctttgcttt gtctgtttct tacttttgcc ctgacgtttt attggatttt tatcgacgat
    35401 actctccgtt taagcggcag gtttccgctg tacgtaaaag aaccggccag taaatatgtt
    35461 ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat tctctggtat
    35521 agtgccagca gtacttttgg caaggattca gacatcgtga tgggcataag agcgcaacaa
    35581 aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag tgctgaacgc
    35641 agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct
    35701 ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga tgagagcggt
    35761 ttaatgctac gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg
    35821 atccgcacct cggtctccac atttatggag ttcatcggta ataatcctaa cgccttccgg
    35881 ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa
    35941 attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca tatgccgcgt
    36001 gcgtttactg aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag
    36061 gcgttggacg tcggcgtcga acaacgtcgg caattagaag agcgactggt actgcaactg
    36121 cgaatgattt cgaaaggggc ttattactgg tatcgccgtg aacaagagaa aaccgcaatt
    36181 attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga ggtacgctgc
    36241 tgctggcgtt agttgctggc ttatcgatta atggtacttt cgcagcgctg tttagctcca
    36301 ttgtgccatt ttctgtattc ccgattattt ccctggtgct gacggtttac tgcctgcatc
    36361 aacgttatct taatcgcact atgccggtag gcttgccggg tctggcagct gcctgtttta
    36421 ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat atcggctcta
    36481 acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg aagatgcgta
    36541 accgtaagca ggaagttgct gagtaatcgg tattatgccg gatgctggcg catccggcat
    36601 gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta ggggaactga
    36661 tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag attcttgcat
    36721 aacgtttccg ggttacagga gatgtacaaa atacacggat acgcctgcac cattttctcg
    36781 gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc
    36841 tttaagtcga tcccttgcag gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat
    36901 tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagctgc gatgttgtat
    36961 tgcgcagcag caaccgacgg cttagcgatt tcggtggcta atacccgatc aaaattgcgc
    37021 gccagcgcta atgaaaagtt accgttgccg cagtacagct ccagtaaatc gccttttgag
    37081 cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc gttcggctgg
    37141 gtaaagctgt tttctacctg acggtagatc atctctttcc ctgcgaccgg cagacgctcg
    37201 tcgatgtaat cttgatccag ctcgattttg gttttcgttg cccgaccaat cagatgcaca
    37261 ttcagattct gcgcgcgcag tgcatcgcgc agggcctccg cttcctgacg ccactcatca
    37321 tccagcttct tatggtatag cagggaaacc accgcctgat tactcagcgt ggtgaggtaa
    37381 tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc aatcatcgcc
    37441 gtcatcaact ggttgataag ttcactggcg gcggggaagc tatccacgcg gatacggctt
    37501 ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca gatgcggaac
    37561 tccgcacgca tccggtaatg actgaccggc gagcgaaaca cttccggaac caggtcagaa
    37621 aacggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc ttcatactgt
    37681 tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt
    37741 gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga gctacgtctg
    37801 gacagtaact tgttacaacc tgtagcatcc acttgtcggt cctgtgagtt aatagggaat
    37861 ccagtgcgaa tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc
    37921 ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc cctccaagcc
    37981 cgaagacctg ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc
    38041 tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt cgctgctgac
    38101 ggcgtgttct gtcacagcct tttccgcttg ggcacaggat accagcccgg atactctcgt
    38161 cgttactgct aaccgttttg aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt
    38221 gacccgtcag gatatcgacc gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct
    38281 tccgggcgtc gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta tttttattcg
    38341 cggtacaaat gccagtcatg tgttggtgtt aattgatggc gtacgcctga atctggcggg
    38401 ggtgagtggt tctgccgacc ttagccagtt ccctattgcg cttgtccagc gtgttgaata
    38461 tatccgtggg ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg tggtgaatat
    38521 catcacgacg cgcgatgaac ccggaacgga aatttcagca gggtggggaa gcaatagtta
    38581 tcagaactat gatgtctcta cgcagcaaca actgggggat aagacacggg gaacgctgtt
    38641 gggcgattat gcccatactc atggttatga tgttgttgcc tatggtaata caggaacgca
    38701 agcgcagaca gataacgatg gttttttaag taaaacgctt tatggcgcgc tggagcataa
    38761 ctttactgat gcctggagcg gctttgtgcg cggctatggc tatgataacc gtaccaatta
    38821 tgacgcgtat tattctcccg gttcaccgtt gctcgatacc cgtaaactct atagccaaag
    38881 ttgggacgcc gggctgcgct ataacggcga actgattaaa tcacaactca ttaccagcta
    38941 tagccatagc aaagattaca actacgatcc ccattatggt cgttatgatt cgtcggcgac
    39001 gctcgatgag atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg
    39061 tagtattggt gcgggtgtcg actggcagaa acagactacg acgccgggta caggttatgt
    39121 tgaggatgga tatgatcaac gtaataccgg catctatctg accgggctgc aacaagtcgg
    39181 cgattttacc tttgaaggcg cagcacgcag tgacgataac tcacagtttg gtcgtcatgg
    39241 aacctggcaa accagcgccg gttgggaatt catcgaaggt tatcgcttca ttgcttccta
    39301 cgggacatct tataaggcac caaatctggg gcaactgtat ggcttctacg gaaatccgaa
    39361 tctggacccg gagaaaagca aacagtggga aggcgcgttt gaaggcttaa ccgctggggt
    39421 gaactggcgt atttccggat atcgtaacga tgtcagtgac ttgatcgatt atgatgatca
    39481 caccctgaaa tattacaacg aagggaaagc gcggattaag ggcgtcgagg cgaccgccaa
    39541 ttttgatacc ggaccactga cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc
    39601 gattaccgac acgccgttgt tacgccgtgc taaacagcag gtgaaatacc agctcgactg
    39661 gcagttgtat gacttcgact ggggtattac ttatcagtat ttaggcactc gctatgataa
    39721 ggattactca tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct
    39781 tgcggttgcg tatccggtca cctctcacct gacagttcgt ggtaaaatag ccaacctgtt
    39841 cgacaaagat tatgagacag tctatggcta ccaaactgca ggacgggaat acaccttgtc
    39901 tggcagctac accttctgaa ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg
    39961 ggttgtcggt ctatgacgag atccggcatc tcttaccgga tctccattac atttatgctt
    40021 tcgataacgt cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg
    40081 tggcaattgt caccgcggtg caagaacgtt atccccttgc gctggctgtg gtcgcttgca
    40141 acactgccag taccgtttca cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg
    40201 gtgtcgtgcc ggcgattaaa cctgctgcac gtctgacggc aaatggcatt gtcggattac
    40261 tggcaacccg cggaacagtt aaacgttctt atactcatga gctgatcgcg cgtttcgcta
    40321 atgaatgcca gatagaaatg ctgggctcgg cagagatggt tgagttggct gaagcgaagc
    40381 tacatggcga agatgtttct ctggatgcac taaaacgtat cctacgcccg tggttaagaa
    40441 tgaaagagcc gccagatacc gttgtattgg gttgcaccca tttccctcta ctacaagaag
    40501 aactgttaca agtgctgcca gagggaaccc ggctggtgga ttctggcgca gcgattgctc
    40561 gccgaacggc ctggttgtta gaacatgaag ccccggatgc aaaatctgcc gatgcgaata
    40621 ttgccttttg tatggcaatg acgccagaag ctgaacaatt attgcccgtt ttacagcgtt
    40681 acggcttcga aacgctcgaa aaactggcag ttttaggctg atttggttga atgttgcgcg
    40741 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    40801 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttct
//
LOCUS       DAJVYM010000017        40665 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVYM010000017 DAJVYM010000000
VERSION     DAJVYM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.017"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            209..1306
                     /gene="mltA"
                     /locus_tag="OQS60_000873"
     CDS             209..1306
                     /gene="mltA"
                     /locus_tag="OQS60_000873"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS2386699.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1383..2189
                     /gene="tcdA"
                     /locus_tag="OQS60_000874"
     CDS             1383..2189
                     /gene="tcdA"
                     /locus_tag="OQS60_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS2386700.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OQS60_000875"
     CDS             complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OQS60_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS2386701.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OQS60_000876"
     CDS             complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OQS60_000876"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS2386702.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4080..4307
                     /locus_tag="OQS60_000877"
     CDS             4080..4307
                     /locus_tag="OQS60_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS2386703.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4658..5575
                     /gene="gcvA"
                     /locus_tag="OQS60_000878"
     CDS             4658..5575
                     /gene="gcvA"
                     /locus_tag="OQS60_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS2386704.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5594..5989
                     /locus_tag="OQS60_000879"
     CDS             5594..5989
                     /locus_tag="OQS60_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS2386705.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5982..7082
                     /gene="rlmM"
                     /locus_tag="OQS60_000880"
     CDS             5982..7082
                     /gene="rlmM"
                     /locus_tag="OQS60_000880"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS2386706.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OQS60_000881"
     CDS             complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OQS60_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS2386707.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OQS60_000882"
     CDS             complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OQS60_000882"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS2386708.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OQS60_000883"
     CDS             complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OQS60_000883"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS2386709.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OQS60_000884"
                     /pseudo
     CDS             complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OQS60_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OQS60_000885"
     CDS             complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OQS60_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS2386710.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13537..14184
                     /gene="fucA"
                     /locus_tag="OQS60_000886"
     CDS             13537..14184
                     /gene="fucA"
                     /locus_tag="OQS60_000886"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS2386711.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14212..15360
                     /gene="fucO"
                     /locus_tag="OQS60_000887"
     CDS             14212..15360
                     /gene="fucO"
                     /locus_tag="OQS60_000887"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS2386712.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OQS60_000888"
     CDS             complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OQS60_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS2386713.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OQS60_000889"
     CDS             complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OQS60_000889"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS2386714.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OQS60_000890"
     CDS             complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OQS60_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS2386715.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OQS60_000891"
                     /gene_synonym="ygdH"
     CDS             complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OQS60_000891"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS2386716.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OQS60_000892"
     CDS             complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OQS60_000892"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS2386717.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21994..22539
                     /gene="syd"
                     /locus_tag="OQS60_000893"
     CDS             21994..22539
                     /gene="syd"
                     /locus_tag="OQS60_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS2386718.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23161..23490
                     /locus_tag="OQS60_000894"
     CDS             23161..23490
                     /locus_tag="OQS60_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS2386719.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23490..24272
                     /gene="truC"
                     /locus_tag="OQS60_000895"
     CDS             23490..24272
                     /gene="truC"
                     /locus_tag="OQS60_000895"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS2386720.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24290..24739
                     /locus_tag="OQS60_000896"
     CDS             24290..24739
                     /locus_tag="OQS60_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS2386721.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25174..26526
                     /gene="gudP"
                     /locus_tag="OQS60_000897"
     CDS             25174..26526
                     /gene="gudP"
                     /locus_tag="OQS60_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS2386722.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26528..27868
                     /locus_tag="OQS60_000898"
     CDS             26528..27868
                     /locus_tag="OQS60_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS2386723.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27889..29229
                     /gene="gudD"
                     /locus_tag="OQS60_000899"
     CDS             27889..29229
                     /gene="gudD"
                     /locus_tag="OQS60_000899"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS2386724.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OQS60_000900"
     CDS             complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OQS60_000900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS2386725.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32274..33575
                     /gene="rlmD"
                     /locus_tag="OQS60_000901"
     CDS             32274..33575
                     /gene="rlmD"
                     /locus_tag="OQS60_000901"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS2386726.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33623..35857
                     /locus_tag="OQS60_000902"
     CDS             33623..35857
                     /locus_tag="OQS60_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS2386727.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35935..36183
                     /gene="mazE"
                     /locus_tag="OQS60_000903"
     CDS             35935..36183
                     /gene="mazE"
                     /locus_tag="OQS60_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS2386728.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36183..36518
                     /gene="mazF"
                     /locus_tag="OQS60_000904"
     CDS             36183..36518
                     /gene="mazF"
                     /locus_tag="OQS60_000904"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS2386729.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36589..37380
                     /gene="mazG"
                     /locus_tag="OQS60_000905"
     CDS             36589..37380
                     /gene="mazG"
                     /locus_tag="OQS60_000905"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS2386730.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37608..39245
                     /gene="pyrG"
                     /locus_tag="OQS60_000906"
     CDS             37608..39245
                     /gene="pyrG"
                     /locus_tag="OQS60_000906"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS2386731.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39333..40631
                     /gene="eno"
                     /locus_tag="OQS60_000907"
     CDS             39333..40631
                     /gene="eno"
                     /locus_tag="OQS60_000907"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS2386732.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10072 a         9840 c        10816 g         9937 t
ORIGIN      
        1 tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt taccccgatc
       61 acggatctgg catgattttg aacaaaaaca aatcactaaa gcctattttt tgtgcaaaat
      121 ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc ttcgttgcgc
      181 cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt accttcttat
      241 gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca
      301 atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag atgccgttgg
      361 cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta attcgtcacc
      421 gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc tgcgcgcagg
      481 cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg aaggtgccga
      541 caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg cgcgccatac
      601 ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg gtcgtctgcc
      661 gtctcgtgcg gagatctacg cgggggcatt gagtgataaa tatattctcg cttacagtaa
      721 ctccctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg actttggtga
      781 tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct atcgcagcat
      841 tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat
      901 tcgtcactgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg aacagaaccc
      961 gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc
     1021 gctggttggt cgcgcgtcag ttgcctctga tcgttccatt attccgccag gtactacctt
     1081 gctggcagaa gtgccgttgc tggataataa cggcaaattt aatggtcagt acgaactgcg
     1141 tctgatggtg gcgctggatg tcggtggtgc aatcaaaggc caacacttcg atatctatca
     1201 agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg gacgtgtctg
     1261 ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc ggctgatgtg ttattctgta
     1321 taccgttttt acggatacga cagggtgagg gataacctca ccctttttaa ttctgaggtg
     1381 ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt
     1441 atggtgaaaa agcgttgcaa ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg
     1501 gtgtcggttc ctgggcggcg gaagcgctgg cgcgcacggg gattggcgca atcacgctta
     1561 tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca aattcacgct ctgcgcgata
     1621 acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat tcgccagatt aacccggagt
     1681 gccgtgtaac ggtggtggat gatttcgtga cgccggataa cgtagcgcag tatatgagcg
     1741 tgggttattc gtacgtgatt gatgccattg atagtgtacg gcccaaagcg gcgctgattg
     1801 cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg tggcgcgggt gggcagattg
     1861 acccgacgca gattcaggtt accgatctgg cgaaaacgat tcaggatccg ctggcggcga
     1921 agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg
     1981 gcgtcgattg cgtgttttct actgaagcgc tggtgtaccc gcagtcagac ggtactgtgt
     2041 gtgcgatgaa agccacggca gaagggccga agcggatgga ttgtgcatct ggatttggcg
     2101 cggcaacgat ggtgaccgcc acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga
     2161 tgatggcgaa agcggcgcgt cagggttaaa caatcaggtc ggatgagacg cgaacgtctt
     2221 atccgacccg gttctatgct taaacctgct tcgtcgcagc gataatcgcc tcgcttaacg
     2281 catttaaccc ctggctgcgt gaggcgctaa gttgcgcacg caatcccagc tcatcaaaca
     2341 atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt cccctcaacg gcagtcaaca
     2401 gcaccgccag caggccgcgc acaatgcgcc cttcgctatc gccaaagaag tgcattttac
     2461 cgttttcagt cacggtatat cccagccaga cgcggttttc gcatccggca atctctttag
     2521 cctgcgcttt taactcgtct ggcaaagccg gaagctgttt ccccagcatg atcaactggc
     2581 gatatttatc ttcccattgc gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta
     2641 cggttgtgcc gaacggatgt ccggcgaatt gcgggttcgt cattaatcca ccaataattc
     2701 cagcgcgcgg tcaacggcat tcaccagcgc atccacatca ctctttgtat tatatggcgc
     2761 aaaagaggcg cgcagtgtgc cggttacgcc taattctgcc agtagcggct gagcgcaatg
     2821 ttgcccggcc cgcagggcaa taccgtactc cgccagcagc gtcaccatat cgctgtgatg
     2881 aacgccagca aaatcaaagg ccagcaggct ggaatcctgg cagcggaatg agcggaagcc
     2941 aggacgtttt gccagcgcat cttccgccag cgtcgccagg ctgcgactcc aactttctgc
     3001 ctggttgata tcgtaatctg ccagccattc cagcgccgcg cttaatccta tgacaccagc
     3061 cacatttggc gttcctgctt ccagtttcca cggcgcagat tgagtcgtga agccgtcaaa
     3121 actcacttcg tgaatcattt tgccgccacc cagccagggc gacatcgctt ccagcagttc
     3181 tgatttgcca tacagcacgc cgatacccgt cgggccatac agtttgtgac cagagaaagc
     3241 atagaaatcg atatctagtt gctgaacatc tgcggggaaa tgcaccgccc cctgagcacc
     3301 atcaaccatc accaccatcc cggctgaatg agcaaaggta atcgctcgcg ccagatccgg
     3361 gcaaccgcca gtaacgttcg acatctgacc caacgccaga atccgactac ggggagtaat
     3421 cagttctggc aacaaatcga catccggcag tcgctgcgca ttaagcggca atttcaccac
     3481 tttggctcca gtttgttggg cgaccatcag ccaggggacg aggttagcgt ggtgttctgc
     3541 cacgctgaca ataatctcat cgcccggttg cagacgcgga cgcgcatagc attgtgccac
     3601 catgttgatg gattcagtgg tgccgcgcgt ccagacgata gttttatcat ccggtgcatt
     3661 cagtaattgc gccactttct ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc
     3721 ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg taaaactgtc gagtggcttc
     3781 aaccacggct tcaggtttaa gcgcggtcgc ggcgctatcg agatagacgc ccgcatcctg
     3841 tagtgcggga aactgggcgc gaaactgcgc gggattaaaa acgttcatgg tactcctcgg
     3901 cttgatgcac cgatcgtgac gcaaaattca ctttgataca aggagagtgc taaccatcag
     3961 aattatctgg atatcctggc gaagccgcgt aagtaacata tgctgaatag tgttaggcgt
     4021 taattttagc ctgttaatca atactattaa gatttattaa tcgctttaag gagaagatga
     4081 tgaaaaagac tgccgcaatt atttctgcct gtatgctgac ttttgccctg agcgcctgtt
     4141 ccggttcgaa ctatgtgatg cacaccaatg acggacgtac catcgtctct gacggcaaac
     4201 cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc aacaaacagc
     4261 agatcaaccg tactgacgtg aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa
     4321 ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac agacagggta
     4381 aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc agaccaattg
     4441 caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac acgcattccg
     4501 ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc tggtagaagc
     4561 tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac agtgtgatct
     4621 aattgttaaa ttaatttaac atcaaagttt aaaagccatg tctaaacgat taccaccgct
     4681 aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc gcgcagcaga
     4741 agagcttttc gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg aggatttttt
     4801 ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag
     4861 ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc gtaaactcca
     4921 ggcccgtagc gccaaggggg cgttgacggt cagtttactc cccagtttcg ccattcattg
     4981 gttggttccg cgactttcca gctttaattc agcttatccg ggaattgacg ttcgaatcca
     5041 ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga tattttatgg
     5101 tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt tattgccggt
     5161 gtgttcgccg ctactgctga caggcgaaaa acccttgaag accccggaag atctggctaa
     5221 acatacgtta ttacatgatg cgtcacgccg tgactggcag acatataccc gacagttggg
     5281 gttaaatcat atcaacgttc agcaagggcc aatttttagt catagcgcca tggtgctgca
     5341 agcggctatt cacgggcagg gagtggcgct ggcaaataac gtgatggcgc aatctgaaat
     5401 cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta
     5461 tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc gccaatggat
     5521 cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac aataatttac
     5581 gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta gcggcttcat
     5641 ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga
     5701 gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg cgatcttagg
     5761 tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta gcgttttcct
     5821 cgcgttaggc acggtgttgt tcagcggcag cctttattgc ctggcgctgt cccatctgcg
     5881 tttgtgggca tttgtcacac cggttggcgg cgtgagcttc ctcgcgggct gggcgttaat
     5941 gttagttggt gctatccgtt taaagcgcaa gggcgtaagt catgaataag gttgtattgc
     6001 tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa gccggccagc
     6061 gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt
     6121 atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca ttaatttttg
     6181 cccgccagtg gtttgtggtg ggggaactct tgcagcattt gccgccagaa gatcgtatta
     6241 cccccattgt cgacatgttg cagggcgtag tagagaaggg cggtgaactg cgtgttgaag
     6301 ttgccgatac caacgaaagc aaagagttgc tgaaattctg ccgtaaattc accgtgccgc
     6361 tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta tgaaacgccg aagcgtccgg
     6421 ttgtgcatgt attctttatt gcaccaggct gctgctatac cggttactca tacagcaaca
     6481 ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagag gcgccgagtc
     6541 gttccacgct caaactggaa gaggcatttc atgtgtttat tcctgcggat gagtgggatg
     6601 aacgcctggc gaacgggatg tgggcggtgg atttaggcgc ttgccctggc ggctggacct
     6661 accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg atggcgcaaa
     6721 gtctgatgga taccgggcag gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga
     6781 cacgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggct aaagttgcgg
     6841 cattgatggc gcagtggctg gttaatggct ggtgccgtga aaccattttc aatctcaaac
     6901 tgccgatgaa aaaacgctac gaagaagtat cacacaatct ggcgtatatt caggcacagc
     6961 ttgatgaaca tggcataaat gctcagattc aggcgcggca gttgtatcac gatcgcgaag
     7021 aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat
     7081 aacaggcaag gccggggcgt cgtcttcggc ctgctaccct ttccgtcagg ctgttaccaa
     7141 agaagttgca acctggcgct catcagaaat aatgtgcgtt acctcatcca gatgcccgat
     7201 gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg attgcgcggc
     7261 acgttttaat agcatcgatt tgtaatcagc gttgatcgca ttggagtccc acagtgcgcc
     7321 gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg atttaagttg
     7381 ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact
     7441 gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc tattggtgaa
     7501 gacctgaatg ttgatgtcag gcaactggcg tgccagatac cagcaagttg aactggcgtc
     7561 taaggctatc accatccctt cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt
     7621 atgcgcataa tggcttttca gcctgatgtg aaagggatcg ccactgtctt gattttgacg
     7681 gtggatatat ttagcgcgtc catgattgcg cagaatttta ccctgcgtct gtaattcatt
     7741 gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag cttccgtggt
     7801 caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt
     7861 tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccgatat gagattacgg
     7921 tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg tgataacgat
     7981 cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg
     8041 acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag gatcgagagt
     8101 gtcaccttct accgctgcca tcatcaccag cggcggggca taactgtcca gctcaaataa
     8161 tgggataatc gcttggagca agtcgctcac caacaggcca tcagcgcgga tcacctgcgg
     8221 tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc catgtcccat
     8281 ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt tcagcatttc
     8341 acacttcctc tataaattca ggttcagttt gcgggtagaa ataacggtac tgataatgaa
     8401 tctgtgcacg ggcttcttcc gggctgttaa attcccctac gccataccaa ccgaacagcg
     8461 cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt taccggaata tcaagcatat
     8521 tggctttaat ctgattccac aatgtgttgc gacttcctcc accgactaac aataattcag
     8581 aggctttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat tgcgcagtta
     8641 acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtc actccttgcc
     8701 agccagcgtt ctgacacgac aataaatcac actgcatttt cacaccatcc gcgccaggtg
     8761 cgatcagacg agcttcttca atcaacattt gccagggtgt ttcagccgtc cagaacagtt
     8821 ttctcaccca ttccagcacg ccggatgcca gccattgcat acctgggtta tacaaccctg
     8881 cctggctatc cagttcgcag gtagaatcgg cgtactgact taacagcgaa gtatcaacct
     8941 gggcgctacg gaccattaaa atttcccatg taccggaaga gagcacgggt tcattttggt
     9001 cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc accggtatgc
     9061 caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc tgttcacccg
     9121 cttccaccag acgagggaag agtcggcgtg gaataccggt ggcttgtaaa atttgcggac
     9181 tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgtg atatcagtag
     9241 tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag gcgtgcgcgc
     9301 gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac gtattgaaac
     9361 taaaggctcc gacgccagaa atagcctgca accgctgtgc ggagatcaac tgttcaatat
     9421 tgtccataac cgctgctgtt cgcggacatt tccagctaat gatcggatag agcagattgc
     9481 cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata ccgcggatgt
     9541 ggcattcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg
     9601 cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg gcattaggcg
     9661 ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg acattggtcg
     9721 cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca atatagccgg
     9781 ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc cggagcgacc
     9841 gggcatcaca tcagggagta atgtattaac gcttgtacaa cggaccgtag ttctggcaag
     9901 cgcggtaatc ctggccttca atatccatgc cgtgcgcagc ccaggcagaa ggacgataca
     9961 ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag gcgagagtga
    10021 taaagtctgc gccaacgtgg ccgatggtca gaaccccatg gttagcgccc cagttcgcca
    10081 ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca aaccaggtgg
    10141 ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc ggcaattcca
    10201 cgctccagcc ttccgcgatt tgcagtaccg atcccaggcc tttgatgatg ttgacacgag
    10261 tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg ccgccacgga
    10321 agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca gcctcttgct
    10381 gagagatttc ccagtgtggc ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg
    10441 aaccgtccag cgcagcagaa cctgagttga tcaaatggat gatgccgtgt tctgccagtc
    10501 catcgagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag gtacgcacat
    10561 cggcaaatac ctgagcggtg ccggtgagct ggtgacccat tagcattgcc acgccgttaa
    10621 gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac gccattccag tcaaatgaac
    10681 tgttgaggat cgcttcggcg gtgtcaccat tgggatattg atcggtccag tgacgttgcc
    10741 cctggaagcc cgcagcgatg gcgttgtagc caagtgattc ttccacgcga ccaatatcgg
    10801 ccagtttgct gttgccttgc atcatgtcgc ggatacacat cgccatcagt aaactttcgc
    10861 gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt tcatcttcgc
    10921 catagcggaa gtttttatca gcccaggtca gtgccatttc cagttcggct tcgtcgtaaa
    10981 tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact ttcattccca
    11041 gccaggattc aaagaagttg tgatcaacaa tagaaccggc gatacccatc gaaacgccgc
    11101 ccagcgacag atagctttta cctttcatgc tggcgacggc caaaccggcg cgggcaaagc
    11161 gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat
    11221 gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga gccgctgcca
    11281 ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc gggcgggttg
    11341 gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta atggtgaggc
    11401 ctacattcta gctgctgaat ttttcttcgc aagcagcggc ttcagccata cccgcgatac
    11461 aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt ttctcggtca
    11521 gcagtgcggc cgtagctttc gccatattca ttgtttgttc ttcaagcgac tcacgaacac
    11581 ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg atttttttca
    11641 ttacagattc ctcactttat tcggaaaata tgttcagtta gttgccgttt gagagcggaa
    11701 acgggcaaag ataaagatga ccgcgaagca gagtgtgggg atcagttcag cagtggggat
    11761 gttgcccgcc gcgtcactga caaaacccat gaccggagtg acaataccgc cgccaataat
    11821 ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct taatgcccag
    11881 cgagaagatt gttgggtact gaatcgacat aaaggcgctg cataaagtca gggctattaa
    11941 gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga ttaatgcgta
    12001 ggcggccagg actttgtgtg gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc
    12061 aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg cagtcatacc
    12121 tggaatttct tctacagcgt agcgaatcaa atagctccag caggccgttt gtgcgccaac
    12181 gtagcagaat tgcgctaata ctgcccagcg ccagtggcga atacgcgcta ggcgagaaag
    12241 cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca atgccgggaa
    12301 tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga tcatataagg
    12361 tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg gagacatttt
    12421 atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagtcttt gcccaaagac
    12481 aaccgcgata attgcgccaa acgagttaaa tgtttgcgca agatttaagc ggaagtgacc
    12541 actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa
    12601 tccggctgca ataataaata ggccaactaa aaataaggtg tagttcatta tttctgcagc
    12661 gggccagaat aatgcagcgc ccagggcata taaaaataac ccagtaataa ttcctgcttt
    12721 ataactgagt tttttcatca atatcccagc aggaattggg ataatgaaat aaccaaagta
    12781 aaaggccgat tggatcaggc cagcctggaa atttgtcagc gtaaaagcct gctggaattg
    12841 aggtaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca gtgagcacag
    12901 cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta ccgcacggta
    12961 actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga agcttaagca
    13021 cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca tatcagtgaa
    13081 tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg ctctgtaata
    13141 tgacggcggt cacacttatt agctggaagg tgactttatg tgactaccat cactttaata
    13201 atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc agatgactgt
    13261 attaattgtt tatgccgaaa acgggcattc ctggcttggg tgaatagaaa aatgaccgtt
    13321 ttgataaata tttttaagag cggtcatttt taatggggtt ataaaaagag aacttgcagt
    13381 tatttactgt gattagttga gctaggtcac aaaatataag cttgtgaatt tctttcttgt
    13441 taatcaggaa gaagatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac
    13501 attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt
    13561 cagattattg acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg
    13621 aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa
    13681 aaactgacgg agtcgcatat tgtctttatt gatggcaacg gtaaacatga ggaaggaaag
    13741 ctcccctcaa gcgaatggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac
    13801 gcggttgttc acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc
    13861 cccgctattc actacatgat tgcggcggca ggtggtaatt ctattccttg cgcgccttat
    13921 gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag
    13981 gcaactttgt tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta
    14041 tggctggcgc atgaagttga agtgctggcg caactttacg tggcgaccct ggcgattacg
    14101 gacccggtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc
    14161 tatgggttac ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac
    14221 agaatgattc tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat
    14281 gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa
    14341 tgcggcgtgg tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt
    14401 tacgacggcg tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc
    14461 cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt
    14521 aaagcgattg gcattatcag caacaatccg gagtttgccg atgtgcgtag cctggaaggg
    14581 ctttccccga ccaataaacc cagtgtaccg attctggcaa ttcctaccac agcaggtact
    14641 gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg caagtttgtt
    14701 tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt
    14761 atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg
    14821 tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc
    14881 attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg
    14941 ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt
    15001 atggcgcatc cactgggcgc gttttacaac actccacacg gtgttgcgaa cgccatcctg
    15061 ttaccgcatg tcatgcacta taacgctgac tttaccggtg agaagtaccg cgatatcgcg
    15121 cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt
    15181 gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt
    15241 gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt ttgtaccggt
    15301 ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac cgcctggtaa
    15361 atgcgctgat gtgataatgc cggatacgac gctagccgcg tcttatccgg tatataagtg
    15421 cattaaattc aatgtgttga taatcgtttg catccggctc gccgttaccg taccaaccgc
    15481 aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac
    15541 agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc
    15601 agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt ggcgcttttt
    15661 ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa
    15721 tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa
    15781 cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta
    15841 tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg
    15901 gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag
    15961 gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct
    16021 ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc atcaaaaacg
    16081 gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg ctggcaggtt
    16141 tcgacacagg gcgatccctg aacggcatga atgcgacgaa taagattcag tgcatcgaca
    16201 ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg
    16261 atgccggaac aggctacggc tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct
    16321 ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg
    16381 cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac tttatcgagg
    16441 cagacgcgcg gctcgctggt acggcgtagc gccatacgtg cggcgttcac cgcttttact
    16501 gccgcaatgg cgttacgctc gatgcatggc acctggacct gtccggcgac cgggtcacac
    16561 gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg cgccgggctt
    16621 gcacctaaca gttctgccag accagccgcc gccattgagc acgccacacc aacttcaccc
    16681 tggcagccga cttcagcacc agaaattgac gcgttcatct tataaagaga accaatggca
    16741 ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat gaacttgtcg
    16801 tagtacgcca gcactgccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg
    16861 cgtccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca
    16921 accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca
    16981 gcacggcgtg gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca
    17041 ataccgccac gcatgacttc ccatacgttc gccaggtgct gttccagctc ttctttgctg
    17101 tgcagcgcca gctcattttt catcatcagg ccagagagtg acagcccggt ttcctgacaa
    17161 tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctattc
    17221 tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctgg
    17281 ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa
    17341 aggttgtctg catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg
    17401 tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg gatggaatcg
    17461 atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat atcagtgtgg
    17521 tggcctttac cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct
    17581 ttaagcagat tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca
    17641 acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata
    17701 ggaaatacat cgtgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag
    17761 ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg
    17821 ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc
    17881 atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg
    17941 ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg
    18001 ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg
    18061 ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc
    18121 gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac
    18181 agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtaca
    18241 cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc
    18301 ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa
    18361 gagatgatcg gagagtggtt gaaagagaac accataaccg gaattgccag ccacagggtc
    18421 atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg
    18481 ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat
    18541 accagaatac tcattgcttt aacgatcatc tgctcaccga agcgaacgat ggtcatcata
    18601 cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc cagctggtga
    18661 gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag gatcgggtag
    18721 atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct
    18781 acaacctcgg tgatgtcttc gcctgggttt ttaccagaca gtacgaagcg agtcaggccg
    18841 cggtgagcaa aaaatgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata
    18901 ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa
    18961 aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca
    19021 atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct
    19081 tttagccagt tcagctcggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga
    19141 tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta
    19201 tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat tatcccaacg
    19261 ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga
    19321 catctgactt cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt
    19381 tagttaatca tggcgataat ttctttttca tagcgataaa atgtggttta tgaaaatagg
    19441 gaaacgtttg cgttacaaca taaattgtat gacatattaa ctgcaatgat atttatcgcg
    19501 tgagggtggt aagaaacatt catataacaa atgaaataaa ttgatgggta tgtgaccaaa
    19561 aacgagaaaa cccggcaaca aacgtcaccg ggggagggag attacgtgca gatttcgtag
    19621 caagggatgt aggctgagcc tggcaacttc atacgatgct gggcaacaaa accctgtagc
    19681 aggtcgtcca tacgacgcat aatctcttta tcgccgttga ttttgtaagg accaaactct
    19741 tcaatggcgc gaataccgac ttcttttacg ttacccgcca caataccgga gaacgcacgg
    19801 cgcagatcgg cagccagcac ttcaacaggt tgatccgggt aaagcttcag attagccata
    19861 ttctcgtgag acggctcaaa cggcatttgc aaatctggcg caatgcgcat tgaccagtta
    19921 aagctgtagg catcgcctgt gtcgcggcga ttttctttca ccagcggcat cgattttttc
    19981 atctgacgag cgacttcagc ggcgtcatca atgatgatgc ggtaatggcg gcgcgcgttt
    20041 tcacccaacg tatgcacgac aaactcgtcc agtacgcgga agtagtcggc gctctctttc
    20101 gggccggtga ggaacaatgg taaaacctga tctttgttgg ccgggttcat taaaattccc
    20161 agcaaataca gcaactcttc tgccgtaccc acaccgccag ggaagataat gatgccgtga
    20221 gcgatacgga caaacgcttc cagacgtttt tcgatatctg gcatgatgat caattcgttg
    20281 accagcgggt taggcggttc agcggcgata atcgacggct ctgtcatacc aataaaacga
    20341 ctgtctttgt aacgctgctg tgcgtgtccg accgcagcac ctttcatcgg cgcttccatc
    20401 gctcccggac cacagccggt gcagatattc agctcacgca ggcccagctg attcccgacg
    20461 cgacgggcat acaaatactc gttttcgtta attgagtgtc cgccccagca gaccaccata
    20521 tttggcgctt caccgacatg cagtgcgcga gcgttacgca agatggaaaa gaccaggtta
    20581 gtgatgtgga cggagttgtc gagattcagg ttgggaaaac gaacggtgtt atggatttgc
    20641 ccgtaaacga agagaatgtc acgcagaacc gcgaacaagt tggcctgcaa cgcgcgaata
    20701 attcgcccat cgacaaaagc ctcttccggg ggattaatca gttccagctt tacgccgcgt
    20761 tcacggcgca ggacgttaat atcgaaattt tcaaaacgag acagcaattc tttgctgtta
    20821 tcggtcaaac taccggagtt cagtacggca agtgaacagt tgcgaaacag ttgatagagg
    20881 tcgctgctgg cggtgcgttt aagcatatcc acttccagct gcgacaacat atccatggag
    20941 ccaagcgggc taatatgtgt aatcaagaaa aactccttat gggacgaata tcgtcattcc
    21001 ctttgattac aatagccctg cctgatgtcc tttcacaacc ttctgcgtga atccagcacg
    21061 caaaattgag aaaaaattta ttgccgaacc agtcttgtgg tcgatgggac aaaatcgcta
    21121 ttactgcgcc acgggttaat gtccagaccg ccacgacggg tataacgtgc gtaaacgctc
    21181 aatttttctg gctggcagaa gcgtaacagg tcattaaaga tgcgttccac gcactgttcg
    21241 tggaactcgt tgtgatgacg gaatgagacc aggtaacgca gcagtttttc tctgtcaatt
    21301 tggcgtccac gatactgaat ttggatcgaa ccccaatctg gttgatgggt gatcaggcag
    21361 tttgatttca gcaggtggct gacaagcgtc tcttccacta ctttttcacc acaggtggca
    21421 ttctccagat agtcagtagt gaattcatag ttatcgatag tgatatcctg gtcatcaatg
    21481 caagtgccat taaaatgacc tatcggctgg ccttccagtt catcaagacg atataacgcc
    21541 acgctaatct taccctgagc gcaagtgctt aagtcgcgct ccagcgtctg gcgcacctca
    21601 tcccagttat taaaacgcgt ctggttaaaa ctgttgagat agagcttaaa actcttcgac
    21661 tcaatcagat ttacgctggt gtaatcaagt tcaacatgac cgacagcgac ctgcggcaaa
    21721 cctttcgcat tcagccagga aagttcatac agcgtccaga tatccgtacc gtgaaaaggc
    21781 aggttatccg ctttcagacc cagcgggtcg cgattcaggc tgcgtggaac gccttgcagt
    21841 aggctggcgt cataggtatc ccggtaatcg gttgattttc caagagtcag gcccgcaagt
    21901 gcctgatggt ttgcataaga agacatgttt caccgtcata aaccagataa actaaacgac
    21961 aagtgtatct ggtttaagtg aagagagaaa ttagtggacg atttgaccgc acaagccctg
    22021 aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag agcataaaag ctggccgtta
    22081 agtgaggaac tgtacggtgt tccttcaccg tgcattattt caactaccga agatgccgta
    22141 tactggcaac cccagccgtt taccggggaa caaaatgtaa acgcggttga acgcgctttt
    22201 gatattgtga tacaacccac aattcatacc ttttatacga ctcagtttgc cggggatatg
    22261 cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa cctggagtga agacgacttc
    22321 cgtcgggtgc aggaaaatct gattggtcat ctggtgactc agaaaaggct aaaactgccg
    22381 cccacgctat ttatcgcgac gctggaagaa gagttagagg tcatctcggt gtgtaatctc
    22441 tccggtgaag tgtgcaaaga gacgctggga acacgtaaac ggacgcattt agcctcaaat
    22501 cttgcggaat tccttaatca acttaagcct cttctgtaat ccaaataccc catctggttg
    22561 tgagagatct cttacagacc ctgtaggaga tcgccaggaa ataagcgaat acttaaaaag
    22621 ataagaatcg ttattttcat ttaaaatcaa aatgttgatt gttaattctt aactttcata
    22681 tgaaattttc cttaaggcat tgtctgtaag cgccttgtaa gacttcgcga aaaagacgat
    22741 tctatcttcg tcgacaggga gtcagacaac gaagtgaaca tcaggatgat gacacttctg
    22801 caggacacac caggatggtg tttcagggaa aggcttctgg atgaagcgaa gaggatgacg
    22861 caggacgcgt taaaggacac ctccaggatg gagaatgaga accggtcagg atgattcggt
    22921 gggtcaggaa ggccagggac acttcaggat gaagtatcac atcggggtgg tgtgagcagg
    22981 aagcaatagt tcaggatgaa cgattggccg caaggccaga ggaaaagttg tcaaggatga
    23041 gcagggagca acaaaagtag ctggaatgct gcgaaacgaa ccgggagcac tgtgaataca
    23101 gtgctccctt tttttattcc tgctatcctt cgcggcagtt tttcttattg aagttgcttt
    23161 atgaccactc atgaccgcgt tcgtctccag ttgcagacgc ttgaagcgtt actgcgtgaa
    23221 catcagcact ggcgaaacga tgaaccccag ccgcatcaat ttaatagtac ccaaccgttc
    23281 tttatggaca ccatggaacc actggagtgg ttgcagtggg tgctgatccc gcgaatgcac
    23341 gatttgttaa ataacaacca gccgttaccg ggcgcttttg ctgttgcacc ctattatgaa
    23401 atggcgctgg cgacggacca tccgcaacgc gcgcttattc tggctgagtt ggaaaaactg
    23461 gatgctcttt ttgcggatga tgcgagctaa tgctggaaat actctatcag gatgaatggc
    23521 tggttgcggt aaataaaccc tccggctggc tggttcaccg tagctggctg gatcgcgacg
    23581 agaaagtagt ggtcatgcaa accgtgcgtg accagatagg acagcatgtt tttactgctc
    23641 atcgtctgga ccgacccact tctggtgtgt tgttgatggg attatccagc gaggccggac
    23701 ggctgctggc acaacagttt gaacagcacc aaatccagaa acgttaccat gcgattgtgc
    23761 gcggctggtt gatggaagaa gcggtgctgg attatccact ggtggaagaa ctggacaaaa
    23821 tcgctgataa atttgcccgc gaagataaag gcccgcagcc agcagtgacg cattatcgcg
    23881 gtctggcgac cgtagaaatg ccggtagcga ccggacgtta cccgaccacg cgctacggcc
    23941 tggtggaact ggagccgaaa accggacgca aacatcagct tcgccgacat cttgcccatt
    24001 tgcgtcatcc gattattggc gatagcaaac atggcgattt acgccagaat cgcagcggtg
    24061 ctgagcattt tggcctccag cggttaatgc tacatgccag tcagttgtca ttgacgcatc
    24121 cttttactgg cgagccgctg actattcacg cgggcctgga cgacacctgg atgcaggcgt
    24181 tatcacaatt tggctggcgc ggtctgcttc ctgaaaatga aagggttgag ttcagtgcgc
    24241 catcgggtca ggatggagag ataagttcct aatccaggga gaaaacggta tggcggaaat
    24301 tggtattttt gtcggcacca tgtacggaaa ttcgctgtta gtggcggaag aagcagaagc
    24361 aattctgacc gcacagggcc acaaagcaac ggtatttgaa gatcctgaat taagcgactg
    24421 gctgccttat caggataagt atgttctggt ggttacgtcc acgaccgggc agggcgacct
    24481 tcctgatagc attgtgccgc tctttcaggg aatcaaagat agtctgggct tccagccgaa
    24541 tctgcgttat ggcgtgattg cgctcggcga cagcagttat gtaaatttct gcaatggcgg
    24601 caaacagttc gatgccttgt tgcaggaaca gagcgctcag cgggttggcg aaatgctgtt
    24661 gattgacgcc agtgagaacc cggaaccgga aacggaatca aatccgtggg tcgaacagtg
    24721 gggcacgctg ttgtcctgat atgttcagcg agcggtaaat gtcgttttag cggtgctgaa
    24781 tcgaatcttt tcaggcaaat gccagtaaaa actgcttcat agcgcggatt tttactggcg
    24841 tttgcctgga gtcaagcgat ccatttcata ctcttcttta tttcttcgtt ttaacccttc
    24901 ctttcttgtt cttgttttca tttccgtgaa gtggattcca ccgtccaggg ctaatgccaa
    24961 aatcgggcct cattgaacgc attaatgttg tgttgttgca cggtgagccg ctatggcgcg
    25021 ctttttatac tgctattgcc agatataaac acgcgccgta ttcggcgaac gacctataaa
    25081 aacggcaaaa aacaccctac gtcacctctg atttcctggc tatgtcgcag tccagagtga
    25141 gcgtggctaa cgcgaatttt caggagtgca acaatgagtt ctttaagtca ggctgcgagc
    25201 agtgtggaaa aacgcacgaa tgctcgttac tggatagtgg tgatgttgtt tatcgtcaca
    25261 tccttcaact acggcgaccg cgctacgctt tctattgccg gttcggaaat ggccaaagat
    25321 atcggccttg atcccgtggg aatgggctat gtgttctctg ccttctcatg ggcttatgtt
    25381 atcgggcaga tccctggtgg ttggttgctg gatcgttttg gttcaaaacg cgtctacttc
    25441 tggtcgatct ttatctggtc gatgtttacc ttgctgcaag gcttcgtcga tatctttagt
    25501 ggattcggca ttatcgttgc cctgtttact ctgcgcttcc tggtcgggct tgctgaagcg
    25561 ccatctttcc ccggcaacag tcacattgtt gcggcctggt ttccggcgca ggaaagggga
    25621 acggcggtgt cgatttttaa ctccgctcaa tacttcgcaa cggtgatctt cgcgccgatt
    25681 atgggctggc tgacgcatga agtgggctgg tcacacgtct tcttctttat gggcggtctg
    25741 gggattgtca tcagctttat ctggttgaaa gtcatccacg agccaaatca acatccgggg
    25801 gtaaatcaga aagagctgga gtacatcgcc gcgggtggcg cgctgatcaa tatggatcag
    25861 caaaacacca aagttaaagt gccgttcagc gtgaagtggg ggcagatcaa acaactgctc
    25921 gggtcacgga tgatgatcgg cgtttatatc ggtcagtact gtatcaacgc cctgacttac
    25981 tttttcatta cctggtttcc ggtttatctg gtgcaggcgc gtgggatgtc gattctgaaa
    26041 gcgggctttg tggcttccgt tccggcggtt tgcggtttta tcggcggtgt gctgggtggg
    26101 attatttccg actggctgat gcgtcgcacg ggatcgctga acattgcgcg taaaacaccg
    26161 atcgtaatgg gcatgttgct gtcgatggtg atggtgttct gcaactacgt caacgttgag
    26221 tggatgatca tcggctttat ggcgctggcc ttcttcggta agggcatcgg ggcgctgggt
    26281 tgggcagtaa tggcagatac cgcgccaaaa gagatcagcg gtctttccgg tggcctgttc
    26341 aacatgttcg gtaacatttc tggcatcgtc acgccaatcg caattggtta tatcgttggc
    26401 acgactggct cctttaatgg ggcgctgatt tatgttggtg ttcatgcctt aatcgcggta
    26461 ctgagctacc tggtgctggt gggcgatatc aagcgtatcg agctgaaacc tgtcgcgggg
    26521 caataagatg acgacacaat ccagtcctgt tattactgat atgaaagtca ttccggtcgc
    26581 cgggcacgat agcatgttgc ttaatattgg tggggcacat aacgcatatt tcacccgcaa
    26641 tattgtggta ctcaccgata acgccgggca taccggcatt ggtgaagcgc cgggcggaga
    26701 ggtgatttat caaacactgg tcgatgctat tccgatggtt ctgggccagg aagttgcgcg
    26761 cctgaataaa gtggttcagc aggtgcataa aggtaatcag gcagccgatt ttgatacctt
    26821 cggcaaaggt gcctggactt ttgaattgcg cgttaatgcc gtggcggcgc tggaagccgc
    26881 cttgcttgac ctgctaggta aggcgctgaa tgttccggtc tgcgaactgt tggggccagg
    26941 caagcaacgc gagactatta ccgtcctcgg ttatctgttt tatatcggtg atcggaccaa
    27001 aaccgatctt ccttatctgg aaaatacgcc gggcaaccat gagtggtatc agttgcgcca
    27061 tcagaaagcg atgaacagcg aagccgttgt gcgtctggcg gaagcctcac aggatcgcta
    27121 tggctttaaa gatttcaaac ttaagggcgg ggtgttacct ggcgagcaag aaatcgacac
    27181 tgttcgtgca ttgaagaaac gcttccctga tgcgcggatt accgttgatc ccaacggtgc
    27241 ctggctactt gatgaagcca tttctctatg caaagggctg aatgatgttc ttacctatgc
    27301 cgaagatcca tgcggcgcag aacagggttt ctccggtcgt gaagttatgg cggagtttcg
    27361 gcgggcgacc ggcttgcccg tcgcgactaa catgatcgcc accaactggc gcgaaatggg
    27421 tcatgcggtg atgctcaatg cggtagatat tccacttgcc gatccgcact tctggacgct
    27481 ttccggtgca gtccgtgtgg cgcagctttg cgacgactgg gggctgacct ggggctgcca
    27541 ttctaataac catttcgata tctctctggc gatgtttacc catgtgggcg cggcggcacc
    27601 gggtaatcct accgctatcg atacccactg gatttggcag gagggcgatt gtcgcctgac
    27661 caaaaatccg ctggagatta aaaacggaaa aattgccgtt cctgatgcgc ccggtctggg
    27721 cgtggaactg gactgggaac aggtacaaaa ggcacatgag gcctataaac gtctgcctgg
    27781 cggtgcgcgt aacgacgcag gtccgatgca gtacctgatc cccggctgga cctttgaccg
    27841 taaacgtccc gttttcggcc gtcattgatt ctgaaaaagg acataaatat gagttctcaa
    27901 tttacgacgt ctgttgttac tgaaatgcag gtcatcccgg tggcgggtca tgacagtatg
    27961 ctgatgaatc tgagtggggc acacgcaccg ttctttacgc gtaatattgt gattatcaaa
    28021 gataattccg gtcacactgg cgtaggggaa attcccggcg gcgagaaaat ccgtaaaacg
    28081 ctggaagatg cgattccgct ggtggtaggt aaaacgctgg gtgaatacaa aaacgttctg
    28141 acgctggtgc gtaatacttt tgccgatcgt gatgctggtg ggcgcggttt gcagacattt
    28201 gatttgcgta ccactattca tgtagttacc gggatagaag cggcaatgct ggatctgctg
    28261 gggcagcatc tgggggtaaa cgtggcatcg ctgctgggcg atggtcaaca gcgtagcgaa
    28321 gtcgaaatgc tcggttatct gttcttcgtc ggtaatcgca aagccacacc tctgccgtat
    28381 caaagccagc cggatgactc atgcgactgg tatcgcctgc gtcatgaaga agcgatgacg
    28441 ccggatgcgg tggtgcgcct agcggaagcg gcgtatgaaa aatatggctt caacgatttc
    28501 aaactgaaag gcggtgtact ggccggggaa gaagaagccg agtctattgt ggcactggcg
    28561 caacgcttcc cgcaggcgcg tattacgctc gatcctaacg gtgcctggtc gctgaacgaa
    28621 gcaattaaaa ttggtaaata cctgaaaggt tcgctggctt atgcagaaga tccgtatggt
    28681 gcggagcaag gtttctccgg gcgtgaagtg atggcagagt tccgtcgcgc gactggcctg
    28741 ccgactgcaa ccaatatgat cgccaccgac tggcggcaga tgggccatac gctctcgtta
    28801 caatccgttg atatcccgct ggcggatccg catttctgga ctatgcaagg ttcggtacgt
    28861 gtggcgcaaa tgtgccatga atttggcctg acctggggtt cacactcaaa caaccacttc
    28921 gatatttccc tggcgatgtt tacccatgtt gccgccgctg caccgggtaa aatcaccgcg
    28981 attgatacgc actggatttg gcaggaaggc aatcagcgct tgaccaaaga accgtttgag
    29041 atcaaaggcg gtctggttca ggtgccggaa aaaccggggc tgggtgtaga aatcgatatg
    29101 gatcaagtga tgaaagccca tgagctgtat cagaaacacg ggcttggcgc gcgtgacgat
    29161 gcgatgggaa tgcagtatct gattcctggc tggacgttcg ataacaagcg cccgtgcatg
    29221 gtgcgttaag acagaaaacc aaaaccggct ctatctggag ccggcttgag actgatgcta
    29281 aacgcaaaat agcctgttgt gctatgtatc aggtctacat cattttttaa ttcattgaat
    29341 ttaattggtt ttgtaggccg gataaggcgt taacgccgca tccggcataa acacaggcac
    29401 tttgtcacca atctgaaacc agcgtcataa aaagccgatt gctactcgac aagacgtccg
    29461 ttacccgaga attttgctgg cttcgcgcgc gacattatcc atctcgtcca acagttccag
    29521 cagctccggc tccaaatctt cttctttagt accgctacgt agctgttgtt cgatcagttg
    29581 gcagagattc ttcatacgcg gcacaccgct atagccgcaa ctgccatgca gtttatgaat
    29641 caaatcaacc aggccttccg ggttttctcc aaccagctgc tcctcaactt tgttgcgaac
    29701 ttcaggcagg aaatcgagta acatttgcag catatcgcgc gctagatcgg tttttcctgc
    29761 tgcctggcgt agtgccagtt gccagtcgag ggtcgcattc gggttcacca caatttcgtt
    29821 gacttcgggc gtcacgacgc gagaggaaat accgctgcca ggcttgtagc gcaacaacaa
    29881 attatgcaat cgctcttctt caatcggttt cgccagataa tcgctcatcc ctgcgccaag
    29941 cagcttctct ttttgcccgg ccattgcatg cgccgttacc gcgataaccg gcgtttgttg
    30001 ctgatggggg agctggtgga tgagctcgca ggcccgaatg ccatccatgt caggcatttg
    30061 aatatccatt aagatcaaat cgaacggcat ctgtttcgcc cgttcaaccg cctgatgccc
    30121 gctatcgcaa agttccacat gttgcaccat atcttccagc aatgcgccga taagtttcag
    30181 gttagcgggg ttgtcatcaa ctgccatgac tgtcattgcc agcttgcttt catcggttac
    30241 aggcaaaagc gtgttttgtt tgtgatgaca aaattccgtc agggcaggca acaggcgcgt
    30301 aggtgttaat ggtttcagca gacacgcgcc gataccatct tgcttgagtt tttctgcatt
    30361 gacttgtgca tggcaaggaa gtgccagcat caggaaatcg gtcatcgata ccgctttcgc
    30421 taatcgctca tgttgcattg ttagcggctc gcggaaggtc accgcgatgc ctaacaacat
    30481 catgtcgtaa tgcgcgggag gcagcgcgga gaacgttggg ctataaacca cttccagcgg
    30541 cgtttcactt aaaatatcca gcgtgcattg cgctgctgcg gagtttggtt cgacataggc
    30601 caggcgctta ccggcgaggc actgggtgga tggcccttcg ataataatgt tcgggttcag
    30661 atcgagatta atgtggaacc agaaagttga accgcgattc ggctggctat ggaacgaaat
    30721 atcgccgccc atttcattaa ccagtttttg tgtaatcacc agccccagac cggtgccacc
    30781 atgacggcgg gaaatactgg catcagcctg tcggaaggcc tggaataagc gcgattgatc
    30841 gcgttcagga ataccaatgc cggtatcccg aatctgcact tcaatctgca ctttggtatt
    30901 actcagcgca cgtttttcta ccagaatatc aatgttgcca ttctcggtga atttaattgc
    30961 attccccacc aggttagtga tgatttgctg taatcgcagt gggtcgccga tcacgttatc
    31021 aggcacgtcg cttttaatat tgagcgtcag ttccaaccct ttatcgtgag aagaatgtgc
    31081 cagcagagta acgacttcat ccagcgtgct gcgtagtggg aatggaatac tttccagaat
    31141 cagcttacct gcttccagtt tcgagaagtc gagaacatca ttaataattg ccagcaaatt
    31201 atttgccgaa cgttcaatcg tattcaggtg atcgcgctgc gttggtgtta attctgtttt
    31261 cagcgtcagg cgggtaaagc caataacacc attcagtggt gtacgcagct cgtgtgacat
    31321 atttgccaga aactcggatt taatacgcgc cgcttcctgg gcgcgctttt tcgccagatc
    31381 taactcaacg ttctgaattt ccatctgctc cagcgtttca cgcagatcgg acgtcgcctg
    31441 gtcgatattg tgctgcatct cttcgtgata agcagccagc gacattgcca tcgagttgat
    31501 accgtttttc agcatatcca gctcgccgag cataaatcct tccacccggc tgtcgagttg
    31561 cccgcgacgg atgcggtcga cggtattcac catgttgcga atcggaccgg ttacatcgcg
    31621 cattaagcgc cagccaaaaa taagcgcaat accgatacaa aacagcatca tcacgctgga
    31681 aataaagatc tctttatatt gctgcaagcg aaccgattta agatccagct ccagcgcaat
    31741 atatcccaac atattttgac tatttttggc atcgctactg ggcgattcgt cgggggagta
    31801 actctcagaa ataatcggcg tgcggaggat cataatatcg ccgtcacgag tgacagtgag
    31861 ctggcgagga aacggcacgt tgctgccgag ctgcattgat gagggatcaa gatgaaaatt
    31921 ggaggtgaca aagagtcggt tattttcatc ataaaccgaa atcgcgcgaa caatatcgga
    31981 atggcgacga tgcagtacgc ttattaactg accgatagat tcgcgatttt gcaggctcat
    32041 gccatattca gtagaaactg caagcggctc aataatgctg gctccggcat cttccagttg
    32101 acgctgcaag tcgttatagc gatgcacaac gaaaaagata ctcagcaata aaccaataag
    32161 gacggtcggt gccaggatca gaatcatcat gcgtgcgcgc aggctgtagt tggtcatgga
    32221 gttccgttat gggacaatta aggtcacact gttaaattga gaaagtctcg ccaatggcgc
    32281 aattctactc tgcaaaacga cgcacgacga cgcgtcagat cataaccgtt tcagtcaacg
    32341 acctcgactc ttttggtcag ggcgtggcgc gacataacgg caaaacgcta tttatccccg
    32401 gattattgcc gcaggaaaac gcggaagtta ctgttactga agataaaaaa cagtatgccc
    32461 gcgctaaagt cgtacgccgg ttaagcgata gcccgggacg cgaaacgcca cgctgtcctc
    32521 attttggcgt atgcggcggc tgtcagcaac aacacgccag cgtggattta cagcagcgaa
    32581 gcaaaagtgc ggcactcgcc cgattaatga aacacgaagt ctctgaagtg atcgccgatg
    32641 ttccctgggg ctatcgccgt cgcgcgcgtt taagtctgaa ctacttaccg aaaacacagc
    32701 aacttcagat ggggtttcgc aaagcgggct ccagtgacat tgtcgacgtt aaacaatgcc
    32761 ccattttagt gccccaactt gaagcattgc tgcccaaagt catggcatgc ttgggcagct
    32821 tacaagttat gcgccatctt ggtcatgttg aactggtaca ggcaaccagc ggcacgctga
    32881 tgattttgcg ccataccgca ccgctaagtt cggcagatcg cgaaaaactg gaacgctttt
    32941 cgcattctga aggcctggat ctgtatctcg cccccgatag tgagatactc gaaaccgtct
    33001 ctggtgagat gccctggtat gactcaaatg ggttgcgctt aacttttagc ccgcgcgatt
    33061 ttattcaggt caatgcgggt gtgaaccaaa aaatggtagc gcgtgcgttg gaatggctgg
    33121 atgtgcaacc tgaagatcgc gtactggatt tgttctgcgg tatgggcaac tttacactgc
    33181 cattggcgac acaagctgcc agtgtggtgg gtgtagaagg cgttccggcg ctggtggaaa
    33241 aaggccagca gaatgcgcgt cttaatggct tacagaatgt gacgttttat cacgaaaatc
    33301 ttgaagaaga tgtcacaaag cagccgtggg cgaaaaacgg cttcgataaa gtgttgctgg
    33361 acccggcgcg agcaggtgcc gcaggtgtta tgcagcaaat tataaaactg gaacctattc
    33421 gtatagttta tgtatcctgt aaccctgcaa cgctggctcg ggatagcgaa gcgttattaa
    33481 aagcaggata taccattgcg cgactggcga tgctggatat gttcccacac acgggacatc
    33541 tggaatcgat ggtacttttc tcgcgcgtta aatagttgcg atttgccgat ttcggcaggt
    33601 ctggtcccta aaggagagga cgatggttgc ggtaagaagt gcacatatca ataaggctgg
    33661 tgaatttgat ccggaaaaat ggatcgcaag tctgggtatt accagccaga agtcgtgtga
    33721 gtgcttagcc gaaacttggg cgtattgtct gcaacagacg caggggcatc cggatgccag
    33781 tctgttattg tggcgtggtg ttgagatggt ggagatcctc tcgacattaa gtatggacat
    33841 tgacacgctg cgggcggcgc tgctgttccc tctggctgat gccaacgtag tcagcgaaga
    33901 tgtgctgcgt gagagcgtcg gtaagtcggt tgttaacctt attcacggcg tgcgtgatat
    33961 ggcggcgatc cgccagctga aagcgacgca cactgattct gtttcctccg aacaggtcga
    34021 taacgttcgc cggatgttat tggcgatggt cgatgatttt cgctgcgtag tcatcaaact
    34081 ggcggagcgt attgctcatc tgcgcgaagt aaaagatgcg ccggaagatg aacgtgtact
    34141 ggcggcaaaa gagtgtacca acatctacgc accgctggct aaccgtctcg gaatcggaca
    34201 actgaaatgg gaactggaag attactgctt ccgttacctc catccaaccg aatacaaacg
    34261 aattgccaaa ctgctgcatg aacggcgtct cgaccgcgaa cactacatcg aagagttcgt
    34321 tggtcatctg cgcgctgaga tgaaagctga aggcgttaaa gcggaagtgt atggtcgtcc
    34381 gaaacacatc tacagcatct ggcgtaaaat gcagaaaaag aacctcgcct ttgatgagct
    34441 gtttgatgtg cgtgcggtac gtattgtcgc cgagcgttta caggattgct atgccgcact
    34501 ggggatagtg cacactcact atcgccacct gccggatgag tttgacgatt atgtcgctaa
    34561 cccgaaacca aacggttatc agtctattca taccgtggtt ctggggccag gtggcaaaac
    34621 cgttgagatc caaatccgca ccaaacagat gcatgaagat gcagagttgg gtgttgctgc
    34681 gcactggaaa tataaagagg gcgcggcagc tggcggcgca cgttcgggac atgaagaccg
    34741 gattgcctgg ctgcgtaaac tgattgcgtg gcaggaagag atggctgatt ccggcgaaat
    34801 gctcgacgaa gtacgcagcc aggtctttga cgaccgggtg tatgtcttta cgccgaaagg
    34861 tgatgtcgtt gatttgcctg cgggatcaac gccgctggac ttcgcttacc acatccacag
    34921 tgatgtcgga caccgctgca tcggggcaaa aattggcggg cgcattgtgc cgttcaccta
    34981 ccagctgcag atgggcgacc agattgaaat tatcacccag aaacagccga accccagccg
    35041 tgactggtta aacccaaacc tcggttacgt cacaaccagc cgtgggcgtt cgaaaattca
    35101 cgcttggttc cgtaaacagg accgtgacaa aaacattctg gctgggcggc aaatccttga
    35161 cgacgagctg gaacatctgg ggatcagcct gaaagaagca gaaaaacatc tgctgccgcg
    35221 ttacaacttt aatgatgtcg acgagttgct ggcggcgatt ggtggcgggg atatccgtct
    35281 caatcagatg gtgaacttcc tgcaatcgca atttaataag ccgagtgccg aagagcagga
    35341 cgccgccgcg ctgaagcaac ttcagcaaaa aagctacacg ccgcaaaacc gcagtaaaga
    35401 taacggtcgc gtggtagtcg aaggtgttgg caacctgatg caccacatcg cgcgctgctg
    35461 ccagccgatt cctggagatg agattgtcgg cttcattacc caggggcgcg gtatttcagt
    35521 acaccgcgcc gattgcgaac aactggcgga actgcgctct catgcgccag aacgcattgt
    35581 tgacgcggta tggggtgaga gctactccgc cggatattcg ctggtggtcc gcgtggtggc
    35641 taatgatcgt agtgggttgt tacgtgatat cacgaccatt ctcgccaacg agaaggtgaa
    35701 cgtgcttggc gttgccagcc gtagcgacac aaaacagcaa ctggcaacca tcgacatgac
    35761 cattgagatt tacaacctgc aggtgctggg ccgcgtgctg ggtaaactca accaggtgcc
    35821 ggatgttatc gacgcgcgtc ggttgcacgg gagttaggca gaaatttgct cgtatctaca
    35881 atgtagattg atatatactg tatctacata tgatagcggt ttgaggaaag ggttatgatc
    35941 cacagtagcg taaagcgttg gggaaattca ccggcggtgc ggatcccggc tacgttaatg
    36001 caggcgctca atctgaatat tgatgatgaa gtgaagattg acctggtgga tggcaaatta
    36061 attattgagc cagtgcgtaa agagcccgta tttacgcttg ctgaactggt caacgacatc
    36121 acgccggaaa acctccacga gaatatcgac tggggagagc cgaaagataa ggaagtctgg
    36181 taatggtaag ccgatacgta cccgatatgg gcgatctgat ttgggttgat tttgacccga
    36241 caaaaggtag cgagcaagct ggacatcgtc cagctgttgt cctgagtcct ttcatgtaca
    36301 acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac gcaatcaaaa ggatatccgt
    36361 tcgaagttgt tttatccggt caggaacgtg atggcgtagc gttagctgat caggtaaaaa
    36421 gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac agttgcccca gaggaattac
    36481 aactcattaa agccaaaatt aacgtactga ttgggtagtg ttactaactc acttctattc
    36541 tggtcacagg tttccacctg acagacccgc aattttcagg acaattcaat gaatcaaatc
    36601 gaccgtttgc tcactattat gcagcgcctg cgcgatccgg aaaacggctg cccgtgggat
    36661 aaagagcaga catttgccac cattgcgcct tacacccttg aagaaaccta cgaagtgctg
    36721 gacgccatcg cccgtgaaga ttttgacgat cttcgcggtg aactgggcga tctgctattc
    36781 caggtggtgt tttacgcgca aatggctcag gaagaagggc gctttgactt taatgatatt
    36841 tgcgctgcta ttagcgataa attagagcgt cgccatccgc atgtttttgc tgatagttct
    36901 gccgaaaaca gtagtgaagt gcttgcccgt tgggagcaaa tcaaaaccga agagcgcgcg
    36961 cagaaagcgc agcattcggc gctggacgat attcctcgta gtttaccggc tttaatgcgg
    37021 gcgcaaaaaa tccagaaacg ttgcgccaac gttggcttcg actggacgac gcttggcccg
    37081 gtggttgata aagtctacga agagatcgac gaggtgatgt acgaggcgcg gcaggctgtt
    37141 gtcgaccagg ctaaactgga ggaggaaatg ggggacctgc tgtttgccac ggttaatctg
    37201 gctcgccatt tagggacgaa agcagaaatc gcattgcaaa aagcgaacga aaaattcgag
    37261 cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg gactggaaat gacaggtgtt
    37321 gacctcgaaa caatggaaga agtctggcaa caggtaaaac ggcaggaaat tgatctctaa
    37381 gggaattacg cggtcaagcg ccatttgtgt cattttttaa atgacaagcg cttgatttgc
    37441 gtcaaaaaca tttaccccaa aggggctatt ttctcactcc tgatttcaat agtgcgctgg
    37501 cgaagaggag ggataatgaa agtttgtggc acaggtcatg ttcgggtata ctgctttccc
    37561 gtcctggtta ttccatcgtc ttttcaacct aacttctcag gttcagcatg acaacgaact
    37621 atatttttgt gaccggcggg gtcgtatcct ctctgggtaa aggcattgcc gcagcctccc
    37681 tcgcagccat tcttgaagcc cgtggcctca atgtgaccat catgaaactg gatccgtaca
    37741 tcaacgtcga tccaggtact atgagcccaa tccaacacgg ggaagtgttc gttactgaag
    37801 acggcgctga aaccgacctg gacctggggc actacgagcg tttcattcgt accaaaatga
    37861 gccgccgcaa caacttcacc acgggtcgta tctactctga cgttctgcgt aaagaacgcc
    37921 gcggtgacta cctcggcgca accgtgcagg ttattccgca catcactaac gcaatcaaag
    37981 agcgcgtgct ggaaggtggc gaaggtcatg acgtagtact ggtagaaatc ggcggtacag
    38041 taggtgatat cgaatccttg ccgttcctcg aagcgattcg ccagatggct gttgaaattg
    38101 gccgtgagca cactctgttt atgcacctga cgctggtgcc gtacatggca gcgtctggtg
    38161 aagtcaaaac caaaccgact cagcactctg taaaagagct gctctccatc ggtatccagc
    38221 ctgacatcct gatttgtcgt tcagatcgcg ctgttccggc gaacgaacgt gcgaagattg
    38281 cattgttctg taatgttccg gaaaaagcgg ttatttctct gaaagacgtc gattccatct
    38341 ataaaattcc gggcctgttg aaatctcagg ggctggacga ttatatttgt aaacgattca
    38401 gcttaaactg cccggaagca aatctgtccg aatgggaaca ggttatcttc gaagaagcga
    38461 atccggtaag tgaagtcacc atcggtatgg tcggcaagta cattgaactg ccggatgctt
    38521 ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa gaatcgtgtc agcgtcaaca
    38581 tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt tgaaatcctt aaaggtctgg
    38641 acgcaatcct cgtacctggc ggtttcggct atcgtggcgt agaaggcatg attacgaccg
    38701 cgcgttttgc gcgtgagaac aatattcctt atctgggcat ttgcctgggt atgcaggtgg
    38761 cgttaattga ttacgctcgc catgttgcca acatggagaa cgccaactct acggaatttg
    38821 tgccagactg taagtacccg gttgtggcgc tgattaccga gtggcgcgat gaaaacggca
    38881 acgttgaagt tcgtagcgag aagagcgatc tcggcggtac catgcgtctc ggcgcacagc
    38941 agtgccagtt ggttgacgat agcctggttc gccagctgta caatgcgccg acaattgttg
    39001 agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt gaaacagatt gaagatgcag
    39061 gtctgcgcgt tgcgggccgt tccggggatg atcagttggt cgagatcatc gaagttccga
    39121 atcacccgtg gttcgtggct tgccagttcc atccggagtt tacttctact ccacgtgatg
    39181 gtcacccgct gtttgcaggc tttgtgaaag ctgccagcga gttccagaaa cgtcaggcga
    39241 agtaagtaaa aaagttagaa cggcaacgcg taccttgggt acgcgttgtt tgtctggagt
    39301 ttcagtttaa ctagtgactt gaggaaaacc taatgtccaa aatcgtaaaa atcatcggtc
    39361 gtgaaatcat cgactcccgt ggtaacccga ctgttgaagc cgaagtacat ctggagggtg
    39421 gtttcgtcgg tatggcagct gctccgtcag gtgcttctac tggttcccgt gaagctctgg
    39481 aactgcgcga tggcgacaaa tcccgtttcc tgggtaaagg cgtaaccaaa gctgttgctg
    39541 cggtaaacgg cccgatcgct caggcactga ttggcaaaga cgctaaagat caggctggca
    39601 ttgacaagat catgatcgac ctggacggca ccgaaaacaa atccaaattc ggcgcgaacg
    39661 caatcctggc tgtatctctg gctaacgcca aagctgctgc tgccgataaa gggatgccgc
    39721 tgtacgagca catcgctgaa ctgaacggca ctccgggcaa atactccatg ccggttccga
    39781 tgatgaacat catcaacggt ggtgagcacg ctgacaacaa cgttgatatc caggaattca
    39841 tgattcagcc ggttggcgcg aaaactgtga aagaagccat ccgcatgggt tctgaagttt
    39901 tccatcacct ggcaaaagtt ctgaaagcga aaggcatgaa cactgcagtt ggtgacgaag
    39961 gtggctatgc gccgaacctg ggttccaacg ctgaagctct ggctgttatc gctgaagctg
    40021 ttaaagctgc tggttatgaa ctgggcaaag acatcactct ggcgatggac tgcgcagctt
    40081 ctgaattcta caaagacggt aaatacgttc tggctggcga aggcaacaaa gctttcacct
    40141 ctgaagaatt cactcacttc ctggaagaac tgaccaaaca gtatccgatc gtatctatcg
    40201 aagacggtct ggacgaatct gactgggacg gtttcgcata ccagaccaaa gttctgggcg
    40261 acaaaatcca gctggttggt gacgacctgt tcgtaaccaa caccaagatc ctgaaagaag
    40321 gtatcgaaaa aggtatcgct aactccatcc tgatcaaatt caaccagatc ggttctctga
    40381 ccgaaactct ggctgcaatc aagatggcga aagatgctgg ctacactgca gttatctctc
    40441 accgttctgg cgaaactgaa gacgctacca tcgctgacct ggctgttggt actgctgcag
    40501 gccagatcaa aactggttct atgagccgtt ctgaccgtgt tgctaaatac aaccagctga
    40561 ttcgtatcga agaagctctg ggcgaaaaag caccgtacaa cggtcgtaaa gagatcaaag
    40621 gccaagcata agactgactt tatctgattt aaaaatgcca gcctc
//
LOCUS       DAJVYM010000018        39771 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVYM010000018 DAJVYM010000000
VERSION     DAJVYM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.018"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(29..104)
                     /locus_tag="OQS60_000908"
     tRNA            complement(29..104)
                     /locus_tag="OQS60_000908"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Lys,seq:ttt)
     gene            complement(153..228)
                     /locus_tag="OQS60_000909"
     tRNA            complement(153..228)
                     /locus_tag="OQS60_000909"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Lys,seq:ttt)
     gene            complement(232..307)
                     /locus_tag="OQS60_000910"
     tRNA            complement(232..307)
                     /locus_tag="OQS60_000910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Val,seq:tac)
     gene            complement(457..532)
                     /locus_tag="OQS60_000911"
     tRNA            complement(457..532)
                     /locus_tag="OQS60_000911"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Lys,seq:ttt)
     gene            complement(697..1488)
                     /gene="cpoB"
                     /locus_tag="OQS60_000912"
     CDS             complement(697..1488)
                     /gene="cpoB"
                     /locus_tag="OQS60_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS2386733.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1498..2019)
                     /gene="pal"
                     /locus_tag="OQS60_000913"
     CDS             complement(1498..2019)
                     /gene="pal"
                     /locus_tag="OQS60_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS2386734.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2054..3346)
                     /gene="tolB"
                     /locus_tag="OQS60_000914"
     CDS             complement(2054..3346)
                     /gene="tolB"
                     /locus_tag="OQS60_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS2386735.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3479..4711)
                     /gene="tolA"
                     /locus_tag="OQS60_000915"
     CDS             complement(3479..4711)
                     /gene="tolA"
                     /locus_tag="OQS60_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS2386736.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4776..5204)
                     /gene="tolR"
                     /locus_tag="OQS60_000916"
     CDS             complement(4776..5204)
                     /gene="tolR"
                     /locus_tag="OQS60_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS2386737.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5208..5900)
                     /gene="tolQ"
                     /locus_tag="OQS60_000917"
     CDS             complement(5208..5900)
                     /gene="tolQ"
                     /locus_tag="OQS60_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS2386738.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5897..6301)
                     /gene="ybgC"
                     /locus_tag="OQS60_000918"
     CDS             complement(5897..6301)
                     /gene="ybgC"
                     /locus_tag="OQS60_000918"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS2386739.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6451..6744)
                     /gene="ybgE"
                     /locus_tag="OQS60_000919"
     CDS             complement(6451..6744)
                     /gene="ybgE"
                     /locus_tag="OQS60_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS2386740.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6744..6857)
                     /gene="cydX"
                     /locus_tag="OQS60_000920"
     CDS             complement(6744..6857)
                     /gene="cydX"
                     /locus_tag="OQS60_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS2386741.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6872..8011)
                     /gene="cydB"
                     /locus_tag="OQS60_000921"
     CDS             complement(6872..8011)
                     /gene="cydB"
                     /locus_tag="OQS60_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS2386742.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8027..9595)
                     /gene="cydA"
                     /locus_tag="OQS60_000922"
     CDS             complement(8027..9595)
                     /gene="cydA"
                     /locus_tag="OQS60_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS2386743.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10191..10418)
                     /locus_tag="OQS60_000923"
     CDS             complement(10191..10418)
                     /locus_tag="OQS60_000923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386744.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10443..12999)
                     /locus_tag="OQS60_000924"
                     /pseudo
     CDS             complement(10443..12999)
                     /locus_tag="OQS60_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(13017..14933)
                     /gene="mngA"
                     /locus_tag="OQS60_000925"
     CDS             complement(13017..14933)
                     /gene="mngA"
                     /locus_tag="OQS60_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS2386745.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15102..15824
                     /gene="mngR"
                     /locus_tag="OQS60_000926"
     CDS             15102..15824
                     /gene="mngR"
                     /locus_tag="OQS60_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS2386746.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15928..16797)
                     /gene="sucD"
                     /locus_tag="OQS60_000927"
     CDS             complement(15928..16797)
                     /gene="sucD"
                     /locus_tag="OQS60_000927"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS2386747.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16797..17963)
                     /gene="sucC"
                     /locus_tag="OQS60_000928"
     CDS             complement(16797..17963)
                     /gene="sucC"
                     /locus_tag="OQS60_000928"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS2386748.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18057..19274)
                     /gene="odhB"
                     /locus_tag="OQS60_000929"
     CDS             complement(18057..19274)
                     /gene="odhB"
                     /locus_tag="OQS60_000929"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS2386749.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19289..22090)
                     /gene="sucA"
                     /locus_tag="OQS60_000930"
     CDS             complement(19289..22090)
                     /gene="sucA"
                     /locus_tag="OQS60_000930"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS2386750.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGTSLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22391..23107)
                     /gene="sdhB"
                     /locus_tag="OQS60_000931"
     CDS             complement(22391..23107)
                     /gene="sdhB"
                     /locus_tag="OQS60_000931"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS2386751.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23123..24889)
                     /gene="sdhA"
                     /locus_tag="OQS60_000932"
     CDS             complement(23123..24889)
                     /gene="sdhA"
                     /locus_tag="OQS60_000932"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS2386752.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24889..25236)
                     /gene="sdhD"
                     /locus_tag="OQS60_000933"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24889..25236)
                     /gene="sdhD"
                     /locus_tag="OQS60_000933"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS2386753.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25230..25634)
                     /gene="sdhC"
                     /locus_tag="OQS60_000934"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25230..25634)
                     /gene="sdhC"
                     /locus_tag="OQS60_000934"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS2386754.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26015..26122
                     /locus_tag="OQS60_000935"
     CDS             26015..26122
                     /locus_tag="OQS60_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386755.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26328..27611
                     /gene="gltA"
                     /locus_tag="OQS60_000936"
     CDS             26328..27611
                     /gene="gltA"
                     /locus_tag="OQS60_000936"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS2386756.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28001..28567
                     /locus_tag="OQS60_000937"
     CDS             28001..28567
                     /locus_tag="OQS60_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2386757.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28618..31076
                     /locus_tag="OQS60_000938"
                     /pseudo
     CDS             28618..31076
                     /locus_tag="OQS60_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31091..31822
                     /locus_tag="OQS60_000939"
     CDS             31091..31822
                     /locus_tag="OQS60_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2386758.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31819..32880
                     /locus_tag="OQS60_000940"
     CDS             31819..32880
                     /locus_tag="OQS60_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2386759.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33032..34078
                     /locus_tag="OQS60_000941"
     CDS             33032..34078
                     /locus_tag="OQS60_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS2386760.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34075..34866)
                     /gene="nei"
                     /locus_tag="OQS60_000942"
     CDS             complement(34075..34866)
                     /gene="nei"
                     /locus_tag="OQS60_000942"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS2386761.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34902..35636)
                     /gene="pxpA"
                     /locus_tag="OQS60_000943"
     CDS             complement(34902..35636)
                     /gene="pxpA"
                     /locus_tag="OQS60_000943"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS2386762.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALETIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35626..36558)
                     /gene="pxpC"
                     /locus_tag="OQS60_000944"
     CDS             complement(35626..36558)
                     /gene="pxpC"
                     /locus_tag="OQS60_000944"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS2386763.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36552..37208)
                     /gene="pxpB"
                     /locus_tag="OQS60_000945"
     CDS             complement(36552..37208)
                     /gene="pxpB"
                     /locus_tag="OQS60_000945"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS2386764.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37231..37974)
                     /gene="ybgI"
                     /locus_tag="OQS60_000946"
     CDS             complement(37231..37974)
                     /gene="ybgI"
                     /locus_tag="OQS60_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS2386765.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38245..39726
                     /gene="dtpD"
                     /locus_tag="OQS60_000947"
     CDS             38245..39726
                     /gene="dtpD"
                     /locus_tag="OQS60_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS2386766.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9636 a        10689 c         9680 g         9766 t
ORIGIN      
        1 gggattatgc tgctttaatc attcaggatg gtgggtcgtg caggattcga acctgcgacc
       61 aattgattaa aagtcaactg ctctaccaac tgagctaacg acccatacgg gtgttacctg
      121 aaagatttct actcggggcc aatctttaag attggtgggt cgtgcaggat tcgaacctgc
      181 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccga gtggtgggtg
      241 atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta
      301 atcacccgat actgcgctgg atactacaaa ctacttccac cgaacccacc gtaattagtg
      361 gtgggtcgtg caggatgact cgcttcgctc gccctacggg ccgttgctga cgcaacgtta
      421 tcctacacgc ttaacatctg tcttcgatgc taaaactggt gggtcgtgca ggattcgaac
      481 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccactttttc
      541 gttgctttcg gtttgtttga tatcccgtgg caacggcggc atatattact gatttcagac
      601 ttgagcgcaa caaaaatttc gatgcagatc actcaactgc ttatgattcg cacgacacga
      661 ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg
      721 tgcctgttta gcgccatcag taccagggta tttactgata acctgctggt atacggcttt
      781 cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg actttaaaca tcgcatcagc
      841 agcctttggt gacttcggat agtttttcac taccgaagca aaatagtacg ccgcatcatc
      901 ttttttaccc ttgttgtagt ttaactgacc cagccaataa ttggcgtttg gcaggtaagt
      961 tgaatcaggg taatttttga tgaaattctg aaatgccacc attgcgtcat cctggcggga
     1021 tttatcctgc accagcgcaa tagctgcatt gtaatccgtg tttgcatcgc cgcttttcac
     1081 cggcgcgcca gcattcgcag taccagcatc agctgtcggc gtcgttgatg ccgccgcacc
     1141 gctttgatcg ctgctggttg attgcgccgc tgcaccaccg ctgctgaggc tgtcgatctg
     1201 caacaggatc tgcttttgcc gctccacgac ctgattcagt tgatactgat tttcctgaat
     1261 ctgaccacgc agggaatcaa tatcggattg attatcagaa agttgttgct ggagttgggt
     1321 taaaagctgg ctgtgagcat tagaaatacg ctcaagttga gtgacgcggt cttcgaccga
     1381 gcctgagccg acactactga ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc
     1441 aaccagtaac gacagactca atagttgatg tctgaagtta ctgctcatgc aattctctta
     1501 gtaaaccagt accgcacgac ggtttttgga gtatgccgct tcgtcatgac ccagtactgc
     1561 aggtttttct ttaccgtaag aaacgatgga gatctggtct gcagaaacgc ctttaccctg
     1621 caggtacatc ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt attccggagt
     1681 accacgttcg tccgcgtgac cttctacggt gactttgtaa gacgggttgc tacgcaggaa
     1741 gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg atatcgtact tgtccagatc
     1801 gaagtaaacg atgttgttct gctgcagctg ttgcatttgc agacgagcct gctcttcgga
     1861 agacatgttg ccgttgccgc cgttcgcatc cataccagtg ccggcaccca gcatgccttc
     1921 gctgccgtca ttgctggcgt tcttgttgga agaacatgcc gcaattgcca taacaggcag
     1981 agcaatcatc agccctttca gcactttgtt cagttgcatt tcaatgattc ctttactatt
     2041 caattaatta ttatcacaga tacggcgacc aggcagggaa tttgacctgt ccatcagttg
     2101 ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa attcagcacg gatcccatcc
     2161 cctgagaaga gctgtagatt accatagtgc cgttaggtgc cagacttggc gtttcatcca
     2221 ggaacgtgga cgacagaact tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct
     2281 gctgcccacc gttggagctg accattacca taaatttacc gtcgctgctg acatccgcat
     2341 cctggttctg cgaaccttcc caggtaatac gttgtggcgc accgccgttg atattcactt
     2401 tataaacctg tggacgaccg gcctggtcag aagtaaatgc caggttctgg ctgtccggga
     2461 accaggtcgg ttcggtattg ttactgcgac catcagtcac ctggcggatc tgaccagaag
     2521 ccaaatccat tacgtacagg ttcaggctac cggttttcga caaggcgaat gccagtttgc
     2581 tgccgtctgg cgagaatgca ggcgcaccgt tgtgacgcgg gaatgaagcc acctgacgta
     2641 cagcgccgtt tgccagcgtt tggataacta gcgcggaacg accgctttcg aaggtcacat
     2701 aagccagttt agaaccgtct ggtgaccacg ccggagacat cagcggctgt ggtgaacggt
     2761 gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg cagttcatac gggaactgac
     2821 cgccgttggt ctgaacaacg taggcaatac gggtacggaa cgcaccttta atgccggtca
     2881 gtttttcaaa cacttcatca ctggcggtat gaccagcata gcgcagccac tgcttgttca
     2941 ctttgtacga gttctgagca agtacagtac ccggtgcgcc gccagtgtca acaagttgat
     3001 aagcaacatt gtaagaacca tccggattcg gagtgacctg accgacaact acagcgtcaa
     3061 tacccagcgc ggaccatgca gctggttgta cttcctgcgc actacccggc tgctgtggca
     3121 gacgagcgcg atctaacgga ttaaatttac cgctgttacg caagtcggca gcaacgatgc
     3181 cgccaatatc ttcaggtgcc gcaccagggc ccgcccactg gaaaggaaca acaccaatag
     3241 gacgaccgga atctacaccg ctgtcgatca caatgcggac ttcagcatgc agaactgatg
     3301 cccacagtat gagaaaacca aatgctactc gtaatgcctg cttcatcata tctcccttat
     3361 ctggaccgat ggcccacgat aatttagcag aatgttaaca aactaaaata cacaaaacta
     3421 ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcg gaacagtcaa catcgcgatt
     3481 acggtttgaa gtccaatggc gcgtttttga acacttcata tactgcctgg cttggtggtt
     3541 tcgggatctt cgcaagttta gctgctgcca acgcagcctg acaaagtgcg ggatcgccac
     3601 cttcaggttt gatatccagt aacataccat cgggtgccag ttttatgcgc agcgtacagg
     3661 ttttgcctgc ataggacgat gcgtcataga acttactttc gatagcagat ttaatctgcc
     3721 cggcatagtt attgatatcg gcccctgatg cgccattgtt tttagtatta ccactcccgg
     3781 cagacgaagc attgttccct ttcgcccctc cccccgtttt cggtgcattc ttaccagagc
     3841 ttagctcacc gaaaatatca tctgcctctg cggcagcttt tgctgcagcg gctttttcag
     3901 ctgctgcttt tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc gctgcttttg
     3961 ccgctgccgc ttttttgtct gcggcggctt tttctgccgc tgctttctta tcagctgctg
     4021 ccttatcagc agccgctttt tctgctgctt ttttgtcggc tgcagctttc tctgctgccg
     4081 ctttctttct tgcttcagct gcagcagctt ctgccgcttc cgctttcttc ttcagtgccg
     4141 cagcggctgc ctcggctttt ttctgcgctt cggctgcggc tttggcggct tctgcttctg
     4201 cttttttctt cgcgtctgcc gccgctttct tcgctgcttc ttccgcagct ttagcatccg
     4261 cttcggcctt cgctttagca tctgccgccg ctttcgctgc cgcctcttcc gcttgcttct
     4321 gctttaactc ggcctgtttt gcggcttctt cagcctgctt tttctgttcc tgagcagcta
     4381 accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc cgcttgtttc tcacgcagtt
     4441 cttcagcagc ctgctgttcc tttatcttgc gctgctcatc agaacgcttc gcgcttgatt
     4501 cctggctttg catgcgtttg tactgctcaa ctaccgcacc tgaatcaacc atgacagcgt
     4561 cgatggacga accaccgccg cctccggctg aagcttctat attctcatcg aacgaactcc
     4621 agagcagcgc cgcaaataag atgacatgca gcactgctga aataattatc gcccgcttga
     4681 gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt ccaaaaactg ttcgcctgtt
     4741 acccgctctc tttcaagcaa gggaaacgca gatgtttaga taggctgcgt cattaaacca
     4801 accgatttca cacccgcact atgtaacaag ttcagtgctt taattatttc atcgtaaggc
     4861 acatcttttg cgccaccgat cagaaagacc gttttcgggt tggccttgaa acggctggac
     4921 acttccgcca ccacctgctc tggtggtaaa cgctccaggc gatctttctc aaccaccacg
     4981 gtgtactgac caataccaga cacttcaaca atcactggcg gattatcgtt actgctcacc
     5041 gcctgtgatt cagtagcgtc tggcagatcg acctccacgc tctgggtgat gatgggcgct
     5101 gtcgccataa agatcagcaa cagcaccagc agtacgtcca gcaacggtac aatgttgatt
     5161 tcggacttga gatcgcgacg acctcgtcca cgcgctctgg ccatggctta ccccttgttg
     5221 ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg taaactcttc cataaagttg
     5281 tcgtaattca gttccagttt gtttacgcgc tggttgaggc ggttataagc cattaccgcc
     5341 ggaattgcgg cgaacagacc aattgcagtc gcaatcaacg cttctgcgat accgggcgca
     5401 accatttgca gtgttgcttg ttttaccgcc ccgagggcga taaaggcgtg catgatcccc
     5461 cagaccgtac caaacagacc aatatacggg ctgatggagc caaccgtacc gaggaacgga
     5521 atgtgcgttt ccagattttc aagttcacgg ttcatggaga tacgcatagc acgcgacgcc
     5581 ccttccacta cggcttccgg cgcatggcta ttggcacgat gcaggcgcac aaactctttg
     5641 aacccgctgt aaaatatttg ttccgaacca gtcagattat cccgtttccc ctgactctct
     5701 tgatagaggc gagagagttc gattccagac cagaatttat cttcaaacgc ttcggcttcg
     5761 cgcgccgctg cgttaagaat acgggtccgc tggataataa tggcccagga tgcgattgaa
     5821 aaaccaatca aaatcaacat gataagttta accagaaggc tagccttcag gaacaaatca
     5881 aggatattca tgtcagtcac tgcttaaact ccgcgacaat agacttggga agcgcacaag
     5941 gcttcatttt gagtgggtca acgcaaacaa ccagaacctc tgcttcattc agcaaagtat
     6001 tctcggcgtt gacaatacgt tgcgtgaaaa ccaaagaggt gccacgcatt gatgttattt
     6061 cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc gtaatattcc accgtcattt
     6121 tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg ctgactgaag tgatgatgac
     6181 gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta actggcgtgg tacaccacac
     6241 caccggcatc ggtatcttca tagtagacgc gaaccggcca tcgaaacagc gttgtattca
     6301 ctttacatcc cggtaatgca acaaaagtta gagcttttaa acgctgctac tatacgcgcg
     6361 cgattgaggt ttgggaatga gggcaagtta agggagcgta aaaatttatg ggcctggagc
     6421 ggcccataat tttttaaaga aaattctgat ttaaaagaag aagaaaatca gcccgacaat
     6481 gagaacaata tcggcaagca atgggcaaaa aatcccttgc caaagaacct tctgcggacg
     6541 aaagcccacg ccgtgaatca caccagcaca gacggcccac atcagcaata aaccatgcca
     6601 gatttccagt tcactggtct tcgcggcaaa acgagatggg tcccaaaaca tacatcctgc
     6661 taacagaagc gccatcacga aggaaagcgc ccgcaggggg cgcttgtcca ttaccgcata
     6721 caaggtcgcg ataatcttac tcatcagatg tcttcttgac cggctttgcc tgattcgacg
     6781 tgttcaagcg ccagcgcggt gattacccca aacgaacagg caagaagcgt tcccagaatc
     6841 catgcgaaat accacatttt tagctcctta cttagtacag agagtgggtg ttacgttcaa
     6901 tatcttcttt ggtgatacga ccgaacattt tccagtaaca ccaagcggtg tagagcagaa
     6961 tgatcggtac cagaaccacc gcaacccagg tcatgacgtt aagcgtcagc tggctggaag
     7021 ttgcatccca cattgtcaga cttgcgttca tcatggtgct ggacggcatc acaaacggga
     7081 acattgcgat accggctgtc aggatgatgc aggccagcgt cagggaggag aacacaaacg
     7141 cccacgcggc tttatccata cgtgcagtca ggatggtcag cagcggaaga accacaccca
     7201 gtgccggaat agcccacaga attggcgtgt tgttgaagtt aaccagccat gcgccagctt
     7261 cacgaaccac ttctttattc agtgggttag aggctgcgta atggtccatt gtcgatttca
     7321 cgacataacc atcgataccg tacatcaccc atacgccagc cagtgcgaaa cagaccagtg
     7381 tcaccagcgc agccacctga gccgttgcac gggtacgcag gtgcagttcg cccacggtac
     7441 gcatttgcag ataggttgcg ccctgagtaa tgatcatccc tacgctcacc acgcctgcca
     7501 gcaggccgaa cgggttaagc aactggaaga agttaccggt gtagtacaga cgcagatatt
     7561 catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa cgctacacca attaccagcg
     7621 gcggaacgaa actaccaatg aagatgcccc agtcccacat gttacgccag cgggtttctt
     7681 caatcttgga gcggtagtca aaaccgaccg gacggaagaa caaagacgcc agcacgagga
     7741 tcatcgccac atagaagccg gagaacgcag cggcatagac catcggccag gcagcaaaga
     7801 gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc ccagtgtggt gcaatggagt
     7861 taatcataat tcgacgctcg gtgtcgttac gaccgaggaa acgggtgagc atgcccaccc
     7921 ccatgtcgaa accgtcagtg actgcaaaac caatcagcag aacgccaacc agcagccacc
     7981 agataaaacg caatacttca taatcgatca tttgacgact cctgtcttag cgtgccggct
     8041 gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt tttcaggctg cttgggccga
     8101 ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag gaacagggta tacaggccgc
     8161 aaatcagcac cattgagaag atgagatcgc ctgcggtcag tgacgagttc gccacagctg
     8221 tcggcagcac ttcaccgata gcccacggtt ggcggccata ttcagccacg aaccagcccg
     8281 cttctacagc aatccacggc agcggaatac cgtacagcgc ggcgcgcaga agccattttt
     8341 tctcgccaat gcggttgcgg atgacactcc agaaagagag cgcgatgatt gccagaagca
     8401 ggaagccaca cgccaccatg atacggaacg caaagtacag cggcgctaca cgcgggatgg
     8461 agtctttggt tgcctgttga atctgcgctt cagtcgcatc agccacgttt ggcgtatagc
     8521 gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct attgaactgg tcacgaaccg
     8581 cctggtcggt agaaccagaa cgcagttgtt cgagcagaga gtacgccttc atcccgttac
     8641 gaatgcgttc ttcatgctgc accatcagct ctttcaggcc gataaccggg gtatccacgg
     8701 aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat cgcaaatttg ttcgtctcct
     8761 cttcctgatc aggaatgccg aacagagtaa aggcagcagg cgcaggttgc gtttcccact
     8821 cggcttcaat agcagccagt ttggttttct gcacgtcgcc catttcgtag ccggattcat
     8881 cacccagaac aataacagac agaacagcag ccataccgaa gctggcagcg atagcaaagg
     8941 agcgtttagc gaaggcgaag tcacgacctt tcagcatata ccatgcgctg ataccgagga
     9001 tgaacatcgc gccagtcaca taaccagacg ctacagtgtg aacgaatttc acctgagcaa
     9061 ccgggttaag caccagctcg gagaagctca ccatctccat acgcatagtt tcaaagttga
     9121 aatcggacgc gattgggttt tgcatccagc cgttcgcaac cagaatccac agtgcggaca
     9181 ggtttgaacc gagcgccacc agccaggtga cacacatatg ctgaacttta cccagacgat
     9241 cccaaccgaa gaagaacaga cctacaaagg tggattcgag gaagaaggcc atcagacctt
     9301 cgattgccag cggcgcaccg aagatatccc ctacatagtg ggaatagtaa gaccagttag
     9361 tcccgaactg gaactccatg gtcagaccgg tagccacacc cagagcgaag ttgataccaa
     9421 acaacttgcc ccagaacttg gtcatatctt tataaatctg tttgccggag aggacgtaga
     9481 ccgtttccat aatggccagc aggaacgcca taccgagcgt cagtggcaca aaaaggaagt
     9541 ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc gactatatct aacatcatga
     9601 ctccttgctc atcgcatgaa gactccgaga gtgaaccccg ttagaaaggg tcacacgcat
     9661 gccccaatag aaatttgttt gaacactttc atcataccat gtgaatgcac acaaaaacgg
     9721 cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat tgatcccacg tatatattac
     9781 gctgcaaaat ccttacaata aacaggtttt tgttgaagca aatttgcatt tttcgacggt
     9841 gatcaattta tagctaaatt accgcctttc agccaatttg atcgagaaca atttatctct
     9901 ttttgatgcc aatttccaag acttatacat tgataaatat caattcctac aaattatgtt
     9961 aatcatgttt caataaggta actttagtga catttatgtt taaaatgtgt gagttataag
    10021 gctgaaatta gtttcggttg gcaatttaaa gataaaaaga gacgaagcaa aaacagcacg
    10081 gttgacctta attctttttt tggtcactgg ctaatacgac gcagagtttc aggattacta
    10141 ataaaggcat aaatccaccc cgccgatatt aacatttaat taacttgtaa ttatattata
    10201 attaactcac ccagggttat tattttgtta aaaataaagc cacatatacg tggcttgaga
    10261 gtgaagataa acgcaaaatt acctgatgcg ctacgcttgc cagtgtctac atcatctctg
    10321 caatatattg aatttgcctg cttttgtaga ccgaataaag cgttcatgcc gtatccggca
    10381 tgaacaaagc gccctttgtc aacaatctgg ccgcgcatat gtggcttcgg tttgattgct
    10441 attcaggcaa gccggtaact gaacgtccgt gactggccgg gtaaaaaagg ccccgataga
    10501 tttgaacctt gattttcctc ggtggtaatg tgttcatcca tcatcgtttc tgagcaagaa
    10561 atcacctcgc gactgaaagc aacagtcgca tcacaggttg ctgattcagc cggattaaac
    10621 agccgtaaaa tcacttcttg tcggtcttca gctttcttaa gtgcgcttat caggcatccc
    10681 actgggggca ttttcaacaa actataactt tccggcacgt tgaatccggc tttgttgagc
    10741 ttcatcgcat cccatgggat tttgttgtaa cactgtactg gagtcagcca tgctcgcgcc
    10801 tgctgagcta caccagcggc ggttggcgta ccggtataac tcaataaact taggcgacaa
    10861 gaaagcagac cacgtagttg tgagtccggg actggcattt taattcccga aggccgccca
    10921 ggccttaaaa gcagatcttc tttgcccagt aagcccacgc cacgcagcaa cgtaatggca
    10981 aaggttttct tctcttcacc gatgacttca aattcacgta acccttcgct aaagacagcc
    11041 atgccgttac gcccttcctg taaggcaaca tagttgagca tattccatac cggaaccggc
    11101 gcttctttcc agccttcttg ctgccagttg ttcattgcac tgtcgttcac ggggcgcgtt
    11161 agcgaaccaa actgcgtatc tgccagaaca ctgtcagtgt taaaaggtgt agggaccagg
    11221 acacgaaggc gatgatcgtc agcctggtta tcaaggttga tatccacatc aatacgcctg
    11281 ctgttatgac taagagtgac aaccaacacc ccccctactc tgccaggctt catgaataat
    11341 atcgcattgc ggtttcgcgt tcgctgcggt aattacccac tcttcttttg cgggtgaata
    11401 gtcatattca tcaccatcat cagagctttc ttcaatttgc aatacccggt catagcgcac
    11461 accgctgtct ttatctacca gttgcagaga accatcctca ttgagcgcaa tctgccagaa
    11521 agcattttcc agaatccctt cagcgtcact ttttgccgca atcacgttgc caggctgatt
    11581 cgcttcaata taaagcgtgc gatagcccat agaagggaca atctggttga tctgaatatc
    11641 aaactccata aagggatcat aattgccata atgaacaatt tgccgatcga ttaggccagg
    11701 atcgatctca cgggcatggc gaataaaata cggtacaggc tgaccgcgat cgtcttgtag
    11761 attaaactgg ctggcgcgca gccgcacagt ggtgttaata acttcttccc gcggccaggg
    11821 catcaggtta aacagcacga gtttgtcggc atcactctgt ggcatgttat cggcaatttt
    11881 gcgcatgtag aaacgcagca gattatccgc catgtcttca gccagttcga agcgggcgac
    11941 aatttcgcga tgaactttgt cactgcaaca acaaccgata ctgtcgtggg catgattttt
    12001 taagatctct ttccacattt tctccagcaa gccgtggtgg tattcaaaac ccaacgtcca
    12061 ggccagcgtt gccagcggtt ccagcagatt aacaatctta ttttcaatac gtgcgtgggc
    12121 aattttgata tccatgcgcg tggaaccgat ggtgcgatgt acgcgcatat atttgccatc
    12181 gataaattca cctttcaggg tggccagact ttcacgctgc gcttcgatct tctcaaatac
    12241 ctcttcaaag cggctcatca caaacttacg ttgagggtag atctcacgta gcttatccat
    12301 cacttcgaag atattttgct gcaatggcat ctggtcatga ccattcggca gcaaaatctc
    12361 tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt tacgtaatcc
    12421 gttttcgtcg gcaggtaagt acttaccgat ggcgtagccc agcggcagca cctgcgccgt
    12481 cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatcagtac cgtggcgctc
    12541 cgaacatccg cgccagaaca tagtgcgggt aatgccaaat ccattgtaaa tatgcggcag
    12601 ttgcccggac atgccaaagg aatccggtaa ataacctatt ttcatcggct cgccaaacgc
    12661 gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag aaacaatcgt
    12721 ggtatcggtc tgggtatacc aggggccgat aatcaactta ccggcttcta cctgtttctt
    12781 cacgcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg ccgtttgccc
    12841 gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga tctcttccat
    12901 attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc gatcccaatg
    12961 catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct ttttgtttat
    13021 ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg ccagatcagg
    13081 agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc aattgccgcc
    13141 ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc ggcaccgggt
    13201 gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga gcccagcaca
    13261 aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc ttcggtaata
    13321 cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc tttaaacagg
    13381 cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt gacggaggcg
    13441 aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa tgcataagcg
    13501 gctttgttca ctggccctcc aaggtcaaag gaacacataa aaccgagaat ggcacccagc
    13561 aacagcgcgt tacttcctga cagaccgttc agccaggcgg taagcgagtt attgatccat
    13621 gcgacaggtt ctcccaccac aaacagcatc agactgcccg ctcccaacgt accgagcacc
    13681 gggtagagat aaaaagtcag gaatccattg aatttactgc taagacgcaa gtgatttttc
    13741 acccagcgca tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag aaacccggag
    13801 ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg tttatctgcc
    13861 agagaatagg tggtataggc cgcgagcacc ggtaccatca aaattccgag cagcccgccg
    13921 cccagcttgc ggtacatcca cagccaggag ttttcttcat taaacagatc ttgtagcccg
    13981 aagatttgcg acagtaatac cgcgaccgcc agcaccgtgc cccccgcgac aattaacggt
    14041 acggcaaaag agattccgct caacagtgcc tgtttcagct ccgttttgac acttttcacc
    14101 ggttgcgtat cttgctgtac ggtacgcgtc tcatcgctac gcttgagggt aagcgcttgt
    14161 tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcactgaaag cgcgggaatg
    14221 ccattaaaac gctcactctc cttgatggcg acttcagccg caaaaataca ggcggttgca
    14281 ctattgagtt gatccgccgt taaacgccct tcaatgccgt tagcgccttg tttttcaacg
    14341 taaacattta cgccgagttt gcgtccggct ttttccagat attccgcagc catataggtg
    14401 tgagcaatac ccgccggaca ggccgttacg cagacgatag ttggcgcgtt ggaaaaagag
    14461 gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaactcatc aggcgtcgtc
    14521 gccgactgta tacgcgcccg aatctcatca tccgcaaggc gcgtggtcag cgctgtcagc
    14581 agttgcatat gcgtagtacc cgcttcattg gggggaatcg ccagcagcac cactaaatca
    14641 actgcttccg ggccatcaac gccttcccac tgaagcggct cgctgagtgt ggcgaccgca
    14701 aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc ttcacctaag
    14761 gcggtcgggc caaggctttc acggcgatac acttcttcca gaaattgctc agtactggaa
    14821 attttcccca gagcagcaag acgttgagtc aacgcgtgga tggcctcttc acggctggta
    14881 aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcagatt catcatgatc
    14941 ctcacttgat cgtttttata gtcgcgccag tgtgcccgat aaaacaatac catcatgtga
    15001 tcaatttcac ttgattgaaa ttatttgtat tgtatttgta ttatttacag gatgaattta
    15061 tcgggcataa tggctaaatt catattaatg agatactaaa aatgggacac aagcccttat
    15121 accggcagat tgccgatcgc attcgtgaac aaattgcgcg tggcgagctt aaacctggcg
    15181 atgcgttacc cactgaatca gcgctacaaa cagagtttgg cgtcagccgg gttacggtgc
    15241 gtcaggcttt gcgccagcta gtcgagcagc agatcctcga aagcattcag ggcagcggga
    15301 cttacgtcaa agaagagcgg gtcaattacg atatttttca gttaaccagt tttgatgaaa
    15361 aactgtcgga ccgtcacgtc gatacacaca gtgaaatcct gatatttgaa gtgattccgg
    15421 ctgacgattt tcttcagcaa cagctacaaa tcaccgcgca ggatcgtgtg tggcatgtga
    15481 agcgcgtgcg ctatcgcaag caaaagccaa tggcgctgga agaaacctgg atgccgcttg
    15541 cgttgttccc ggatctcacc tggcaggtca tggaaaattc gaaatatcac tttattgaag
    15601 aagtgaagaa gatggttatc gatcgtagcg aacaggaaat cattcctctg atgccaaccg
    15661 aagagatgag ccgtctgctt aatatcagcc agacaaagcc gattcttgaa aaagtatcgc
    15721 gtggatattt agttgatggt cgggtgtttg aatatagccg caacgctttt aataccgatg
    15781 actataaatt taccctgata gcgcagcgaa aatcatcgcg ataagcacaa aaaaggccat
    15841 catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta gagggaagcg
    15901 gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg cttcaccgat
    15961 atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga atttctcatc
    16021 cgcagtccct ttcccaccag caatgatcgc acccgcgtgg cccatacgtt tgcctttcgg
    16081 cgcagtcaca ccagcgatgt aacctacaac tggcttggta acgtgctctt tgatgtacgc
    16141 tgccgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg cttcggtctg
    16201 cggatctttt tcgaacattt cgaggatgtc gataaagtta gagcctggga tcgggtcacc
    16261 gccgatacca acacaggtcg actgaccgaa accgtaatcc gtggtctgtt taaccgcttc
    16321 ataggtcagt gtaccggaac gggaaacgat acccacttta cccggtttgt gaatgtgacc
    16381 cggctggata ccgattttgc attcacccgg agtgataacg cccgggcagt tcgggccgat
    16441 catacgaacg ccggcttcgt ccagtttcac tttcacagtc agcatatcca gcgtcgggat
    16501 gccttcagtg atggtgataa tcagtttgat gcctgcatca atagcttcca gaatggagtc
    16561 tttgcagaac ggtgctggta cgtagataac agaagcggta gcgccagtag cagcaacagc
    16621 ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta gtaccgcctt tacctggggt
    16681 tacgccgcca accattttag tgccgtatgc aatggcctgt tctgagtgga aagtcccctg
    16741 gctaccagta aagccctggc agataacctt ggtgttttta tcaattaaaa tggacattat
    16801 ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt tgctgcaata
    16861 atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt accttccaga
    16921 cgtaccacga ccggtacgtt aacacccact tctgctaccg cgccgatgat accgtcagcg
    16981 atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt cactttgtcg
    17041 tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc gccgccaacg
    17101 tcaaggaagt tagccggttc gccgccgtgc agtttaacga tgtccatcgt acccatcgcc
    17161 agacctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta gttcagttcc
    17221 cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat ttcgcgcaga
    17281 tcaggctggc ggaacagtgc gttgccgtca gcgcccagtt tgccgtcgag gcaaatcaga
    17341 tcgccctgtt tggtgatgac cagcgggttg atttcgatca acgccaggtc gcgctccagg
    17401 aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt accttccaga
    17461 cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag cggatcaagc
    17521 gcaactttgt ggatcaggtg cggagtttct tccgccactt tttcgatttc cacgccgcct
    17581 tcggtggagg ccataaagac cacacgacgg gaactacggt ctacaacggc accgagatac
    17641 agctctttag cgatatcggt cgctgcttca accagaatct ggttaaccgg ttggccattg
    17701 gcatctgttt gatacgttac cagacgcttg ccgagccagt tttctgcaaa agcacggatg
    17761 tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc agcgtgaacc
    17821 tgacatttca ctacccacgg gccggcaccg atttttgaag cggcttcttc tgcttcgcgc
    17881 ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa aagttgtttt
    17941 gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt tatcttttaa
    18001 accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt aaactactac
    18061 acgtccagca gcagacgcgt cggatcttcc agcaactctt tgatcgttac caggaagccc
    18121 acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta catcatcggc
    18181 aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg catacccaga
    18241 attgcgctct gcggcgggtt gatgatcggc gtagacatca gggaaccgaa cacaccaccg
    18301 ttggtgatgg tgaagttacc accggtcaga tcttcaaccg tcagcttgcc gtcacggcct
    18361 ttaactgcca gctctttgat tttcttctcg atgtctgcca tgccgagggt atcgacatca
    18421 cgcagtaccg gcgtcaccag gccgcgcggc gtggaaaccg ccatgctgac gtcgaaatag
    18481 ttgtggtaaa ccacgtcatc accgtcgata gaagcattca cttccgggta acgtttcagg
    18541 gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc gtggcgtttt
    18601 tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt gacttcgttg
    18661 aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc cacacgctta
    18721 cgcaggcgag tcatcgggac acgtttttca ctacgtgcag ccagagccgg ttgcgccgcc
    18781 ggagcagccg ctgccggagc agactctttc gccggggctt tcgccagatg tttttccaca
    18841 tcttcacgag tcagacgacc acccacaccg gtgcctttaa tggcgctggc gtcgagattg
    18901 tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg ctcttccaga
    18961 gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc gctggtttct
    19021 ttaccggcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt taccgttgta
    19081 ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac ttccagtacc
    19141 actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc gccgggtttt
    19201 ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg gaccagaata
    19261 tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca ttaaccagat
    19321 cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg gaggccgtgc
    19381 ggcctgcata acgcagagaa gtcccaaacg gaatcacttc acggaaatga tgctggctgc
    19441 agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc ttgacgtgag
    19501 caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt tgctcgatac
    19561 gcacaatggc gacatcgtgt tgattgttct tacgacgctg ttccagcagg tcgtaataaa
    19621 ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg tcgatttcac
    19681 cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggaaacc gccagcggat
    19741 gacgcagcag ggatttcggc gacatcacta ccagcggacg acgcatcccg cgcagcgcct
    19801 gacgacgcag catgtggtaa acctgtgccg gggtagacgg aacgcaaacc tgcatgtttt
    19861 gctcagcaca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc ggcccctgcc
    19921 cttcgtaacc gtgcggcagc aacatcacca gaccacacat ccggccccat ttctgttcgc
    19981 cagaagagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca ccgaactgcg
    20041 cttcccagat ggtcagagtg cgtggttctg cggtggcata accgtattca aacgccagca
    20101 ctgcttcttc agacagtacg gagtcccaga cgcggaacgc gccctgcccg ttatggatgt
    20161 gttgcaacgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg tggcggtgga
    20221 agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct tcgtcaacca
    20281 gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca ccggcagcca
    20341 tcgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact gcttccggca
    20401 ccgtgctgat gcgtttcgcc agctcctgca ggcgcttcat ctcaactttg ttcgggtact
    20461 cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg ttcatcggac
    20521 gccactctgc cactacgcaa tcgccagcat ccagcgcatc gcggtacagg ttaaccatct
    20581 cggtggcatc ttccagcgtc gccacttttt cctgctccag cttgtcagcg tagattttgc
    20641 gcggtgtcgg atgttttttg attttctgat acatcagcgg ctgggttgcg ctcggctcgt
    20701 cggcttcgtt gtggccgtga cggcggtagc acaccaggtc gatgaagaca tcacgtttaa
    20761 aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc ggatcatccg
    20821 cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat atcagtacag tacggtgtag
    20881 aacgagcatc cagtggatta gaggtggtga aaccaacctg gttgttgata acgatacgta
    20941 ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagagtttcc tgaaccacgc
    21001 cctgcccggt cactgcggcg tcaccgtgga tggtgattgg cagcactttg ttgctgctcg
    21061 gctcatcaag tctgtccaga cgggcacgaa cagaaccgat aactaccggg cttacaatct
    21121 caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg gtctggaagt
    21181 cagacgagaa gcccatgtgg tatttcacgt cacccgtgcc gaggtgttct ttatgtttac
    21241 cggcaaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc agcacgttca
    21301 gacgaccacg gtgcgccatc ccaagaacca cttcgcgggt gccgctgttg ccagcgtggc
    21361 ggatcatctc tttaagcatc gggattaacg cgtcaccgcc ttccagcgag aagcgttttg
    21421 cgccagggaa ttttgcgccg aggtaacgtt caaggccttc agcggcggtc agttcgctta
    21481 agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata cgctgttgga
    21541 tccagcgttt ttcttcggtg ctggtaatgt gcatatactc ggcaccaatc gggccacagt
    21601 aggtttgctt gagggcttcc agcagctcgc cgagtttcat gttttctttg ccgctggcaa
    21661 atgaaccgac gttgaaggtc tcctggaagt ctgcttcggt cagatcgtgg aaagacggat
    21721 ccagatcggc cactttatct tgctgccaca gtcccagcgg atcgagattc gcatgctggt
    21781 gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc acattggtgt
    21841 cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg cggaaatatt
    21901 cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac tgctggaacg
    21961 tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca tagagctgtt
    22021 ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct ttcaaagcgc
    22081 tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt ggatactacc
    22141 acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctatgt agtggctcgc
    22201 gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt tcctgcattt
    22261 ctttgttacg cctgatgcgc tgcgcttatc aggcctacgg gtaatgactt gtaggccgga
    22321 taaggcgttc acgccgcatc cggcactggt tgcctgatgc gacgcttgcg cgtcttatca
    22381 ggcctacggt ttacgcatta cgttgcaaca acatcgactt aatatggccg atggcgcgcg
    22441 tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga cagcggaata
    22501 cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca cggctatcga
    22561 tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg ggattccacc
    22621 agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac agcccgtcga
    22681 gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt tgtccattat
    22741 tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg tctaccacca
    22801 aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg cccggctggt
    22861 tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg ttcagaccgt
    22921 cggaaccgca cacaccttca cggcaggagc ggcggaacga caggctggga tctttctctt
    22981 tcagctggat aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct tccagggggt
    23041 aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt gaaaactcga
    23101 gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat gccgggcgca
    23161 gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactctggc agatacaggg
    23221 agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta tgtgcgccac
    23281 ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc atcaggttat
    23341 ccagttccaa gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca tccagacggg
    23401 catttttcag acgctcgcgg atcactttca actgctcaag ccctttcgcc atcgcatcac
    23461 cttcacggaa gaccgagaag ttatgctgca tacattcttg cagcgcttta cgaatcgcca
    23521 ccggatcttc accgttacga ttattgttcc agcggtgcag acgatccaga gatgcttcaa
    23581 catcagactc gctggcatcg cgcaatgcgc cctgttcggc gatagactct tgcagatgca
    23641 gacctgccgc acgaccaaag accaccaggt ccagcagcga gttgccgccc agacggttag
    23701 cgccgtgtac cgatacacaa gcgatttcac caacggcaaa cagccccgga acaaccacat
    23761 cttcgccttt ctcgttcacc gtcagcgcct gaccggtcac tttggtcgga ataccgccca
    23821 tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatcg acgtgagcaa
    23881 aggtacggga aagctccagg atacccggca gacgggattc gagaacttct ttacccaggt
    23941 gatcgagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga ccttcacgga
    24001 tttcaatcat gatggaacgc gccaccacat cacggcccgc caggtctttg gcgttcggcg
    24061 cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct tcaccacggc
    24121 aaccttcggt gaccagtacg cccgcaccgg caatgccggt cgggtggaat tgccacattt
    24181 ccatatcctg caccggtacg cctgcgcgga tagccatgcc gacaccgtcg ccagtgttaa
    24241 tgtgggcgtt agtggtggac tgataaatac gccctgcccc gccagtcgcc agcacggtag
    24301 cgcgggcttt gaaataaacc acttcaccgg tttcgatgca cagtgcggta caacccacca
    24361 ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa atggtggtgt
    24421 ggtttttcag gttctgctga taaagcgtgt gcaacagtgc gtgaccggta cggtcagccg
    24481 ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg ccaaacggac
    24541 gttgatagat acgaccatca tcaagacgcg agaacggcag gcccatgtgt tcgagttcca
    24601 gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca ccgatatagt
    24661 ccgacccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg gtattaccca
    24721 gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg aagactttag
    24781 agagcagcgc acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc atacctgcgc
    24841 caccggcacc aatcacaact gcatcaaatt ctctgactgg caatttcatc acacacccca
    24901 caccacaacg aatccataaa tcacgtaaac caccagtgca acgacaatca ccagttgcag
    24961 catcaggcgc aatgccagcg gtttaacgta gtcggtcaac acctgccaca tgccgatcca
    25021 ggcatgaatc aagatagaaa acagcgccag cagggtgaac actttggtga acgcagaggc
    25081 gaagaaaccg atccagactt catatgtcag ttcgccactg gtggcgaaaa aaccgaccat
    25141 ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga aatcatgtac
    25201 gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc gagaagtgaa
    25261 agcacgacag taataacaaa ggagattttg gcggagcgtt tacccgcttc gaatgtttct
    25321 tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg atacgccaga
    25381 gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat cgcggaagct
    25441 tgctcgaaac cttcagggga agagaggctg gtacccagaa gccacagcag gatgcccact
    25501 gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc cgtgatgggg
    25561 aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt tcttatcatg
    25621 aataacgccc acatgctgtt cttattattc cctggggact acgggcacag aggttaactt
    25681 tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt cagacagcgt
    25741 cctttctata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac gacacgttac
    25801 aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt ataggctgtt
    25861 cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg aacagtgatc
    25921 caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa tcattcaaca
    25981 aagttgttac aaacattgcc aggaaaagca tataatgcgt aaaagttatg aagtcggtat
    26041 ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat tcgggacagt
    26101 tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg ccatttattc
    26161 gtcatcaatg gatcctttac ctgcaagcgc ccagagctct gtacccaggt tttcccctct
    26221 ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg cgaaggcaaa
    26281 tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg gctgatacaa
    26341 aagcaaaact caccctcaac ggggacacag ctgttgaact ggatgtgctg aaaggcacgc
    26401 tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtgttc acctttgacc
    26461 caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat ggtgatgaag
    26521 gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct aactacctgg
    26581 aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat gacgaattta
    26641 aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg ttccatgctt
    26701 tccgtcgcga ctcgcatcca atggcagtca tgtgtggtat taccggcgcg ctggcggcgt
    26761 tctatcacga ctcgctggat gttaacaatc ctcgtcaccg tgaaattgcc gcgttccgcc
    26821 tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt ggtcagccat
    26881 ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt cctgaatatg atgttctcca
    26941 cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac cgtattctga
    27001 tcctgcacgc tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc gctggctctt
    27061 cgggtgcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg ggacctgcgc
    27121 acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aattagctcc gttaaacaca
    27181 ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg atgggcttcg
    27241 gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa acctgccatg
    27301 aagttctgaa agagctgggc accaaagatg acctgctgga agtggctatg gagctggaaa
    27361 acatcgcgct gaacgacccg tactttatcg agaagaaact gtacccgaac gtcgatttct
    27421 actctggtat catcctgaaa gcgatgggta ttccgtcttc catgttcacc gtcattttcg
    27481 caatggcacg taccgttggc tggatcgccc actggagcga aatgcacagt gacggtatga
    27541 agattgcccg tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt aaaagcgata
    27601 tcaagcgtta atggttgatt gctaagttgt aaatatttta acccgccgtt caaatggcgg
    27661 gttaattttt atatgcccaa acacaaaaaa ttgtaaaaat aaaatccact atcggacatt
    27721 catatattct aaaaaaaata tatcggtata aattataaac aaatcgatga tttttaatca
    27781 tctatcttcc tctcagttac atgaataaac caccaaatat agcgcacccc tataatttcc
    27841 aatactttat tcatcaaaca tttaattcat accatcaatt aattactttt acatttaata
    27901 aaattaataa tatttttaag aaaaactaaa aacattcgac aatcaaataa gatattcttg
    27961 caccgatata cttttttctt ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac
    28021 attggccgca ctggccgtat ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg
    28081 atccggtgaa attcacttta aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga
    28141 tgatattgat aaagaggttg aactcggtca ggtgaccacc agccacatta atcagtcaca
    28201 tcacagcgat gccgttgctg tcgacctgcg cttagtcaac tgtgatctgg aaaactccag
    28261 caacggttcc ggtggcaaga tttctaaagt tgcagtcacc tttgatagct cagcgaaaac
    28321 caccggcgca gatccgattc tcaacaacac cagcacgggt gaagccaccg gcgtcggcgt
    28381 acgtttaatg aataaagatc aaagcaatat cgttttaggc accgctactc cagatatcga
    28441 cctggctccg acctccagcg aacaaacgct gaatttcttt gcctggatgg aacaaattga
    28501 tcaggctaca cctgtaacgc caggcgcagt tacggcgaat gcaacctacg tgctggatta
    28561 taaataagta ttactttggc ggcgctatcg ccgcctttca taaaatattt atcaggtatg
    28621 gacaccgtga atatttatcg actctctttt gtatcctgcc tggtcgtggc gatgccttgc
    28681 gcattggcgg tcgaattcaa ccttaatgtt ctcgataaat cgatgcgcga ccgcattgat
    28741 atttcattat taaaggaaaa aggagtcatt gctcccggtg agtattttgt tagcgttgcg
    28801 gtaaataata accaaatcag taacgggcaa aagattaact ggcacaaaaa tgacgataaa
    28861 accattccgt gcatcaatga tttactggtc gataaatttg gcttaaaacc tgaagtccgt
    28921 cagtcgttac cattgataaa tcagtgcgtc gattttagct cccgacctga aatgctcttc
    28981 aatttcgatc aagccaatca gcaactgaat attagtattc cgcaagcctg gctggcgtgg
    29041 cactcagaaa actggacccc accctccaca tggaaagaag gtgtcgccgg tatcctgatg
    29101 gattacaact tgtttgccag cagctaccgc ccacaggacg gcagcagcag cactaacctg
    29161 aacgcctacg gtaccaccgg aattaacgcc ggggcatggc gcttacgtag tgattatcag
    29221 ttgaatcaga ctgatagcga tgataaccat gaacagtcag gcggaatatc gcgcacctat
    29281 ctttttcgtc cattaccgca attaggctct aaattaaccc tcggcgaaac ggattttagt
    29341 tccaatattt tcgacggttt ttcttatacc ggcgcggcac tggcaagtga cgagcgaatg
    29401 ttgccatggg aactacgcgg ctacgcccca caaattagcg gtattgcaca gaccaatgcc
    29461 acggtgacga tcagtcaatc aggccgcgtc atttaccaga aaaaagtccc accaggccca
    29521 tttatcattg acgaccttaa tcagtctgtt cagggcacac tggatgtcaa agtgacggaa
    29581 gaagatggtc gggtgaacaa tttccaggtt tcggcagcat cgacgccctt cctgactcgt
    29641 cagggacagg ttcgctataa actggccgcg ggtcagccac ggccctccat gtcacatcaa
    29701 actgaaaatg aaaccttttt tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg
    29761 ctgtacggcg gcctgctgct ttctggtgat gactaccatt ctgccgcaat gggtattggg
    29821 caaaatatgc tgtggcttgg tgcgctgtcg tttgatgtca cgtgggccag tagccatttt
    29881 gatactcagc aggacgagcg gggcttaagc taccgtttta attacagcaa acaagtggat
    29941 gccactaaca gcacgatttc cctcgccgct tatcgtttct ccgatcgtca ttttcacagc
    30001 tacgccaact atctggatca caaatacaac gacagcgatg cgcaggacga aaaacagacg
    30061 atcagcttat tgtgggccaa ccgattaccc cactaaacct caatctttac gccaacctgc
    30121 tacatcaaac ctggtggaat gcagacgcct ccacgaccgc caacatcacg gcaggtttta
    30181 atgttgatat tggtgactgg agagatatct ctatttcgac gtcattcaat acaacccatt
    30241 acgaagataa agatcacgac aaccagattt acctgtcgat ttcgctcccc ttcggtaacg
    30301 gtggtcgggt tggctatgac atgcaaaaca gtagccacag caccacacac cgcatgtcgt
    30361 ggaacgatac gctggatgaa cgtaatagct ggggcatgtc tgccggactg caatccgacc
    30421 gtcctgacaa tggagcccag gtgagcggta actatcagca cctgagttca gcgggtgagt
    30481 gggatatttc tggtacctat gccgccaatg attacagttc cgtcagcagc agctggagcg
    30541 gttctttcac cgcaacccaa tatggtgcag cgtttcatcg ccgcagctcc accaatgaac
    30601 cacgcctgat ggtcagcacc gatggcgtgg cagatattcc ggttcagggc aatctcgact
    30661 acaccaacca ttttggcatt gcggtggtgc cgttgatttc cagttaccag ccttccaccg
    30721 tggcggtgaa catgaatgac ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag
    30781 aaacgtggat tgaaggcgcg ataggttaca aatcactggc ttcccgttcc ggtaaagacg
    30841 ttaacgtcat cattcgcaac gccagcggtc agtttcctcc cctcggagcg gatatccgcc
    30901 aggatgacag cggcattagc gtggggatgg ttggcgagga aggacatgcc tggttaagcg
    30961 gagtcgctga aaatcaaaag tttaccgtgg tctggggtga tagccagcat tgctcgctcc
    31021 atcttcctga acatatggaa gacaccgcaa atcgcctgat tttaccttgt cattaattaa
    31081 ggaaatgaaa atgacattta tgaaaggact accccttttg ttgttggttg ccagtctgtg
    31141 cagccacgct gcactacaac ccgatcgcac tcgtattgtg tttaacgcta atgataaagc
    31201 taccagtctg cgggtggata atcgtagcga taaactcccc tacctcgctt attcctggct
    31261 cgaaaatgaa aaaggggaga aaagcgatga tcttctggtc gccctgccgc cgatacaacg
    31321 tctggaaccg aaagcaacaa cgcaggtacg gatagtaaag caagcctcga ccacaaaatt
    31381 accgggcgat cgcgaaacgc tatttttcta caatatgcgt gaaattcctc ccgccccgga
    31441 aaaaaacagc gaccatgccg ttctgcaagt cgccattcag agtcgtatca aggtgttctg
    31501 gcgaccggct gcattacgca agaaagcagg agaaaaggtc gaactgcagt tacaggtcag
    31561 ccagcagggc aaccaactga cactgaaaaa tcctaccgcg tattatctga ccattgccta
    31621 cctgggacga aatgaaaaag gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt
    31681 tagtacggtc aacacaaaca ccggaaatta tagcggcagc cagttttatc tcggttatat
    31741 ggatgattac ggcgcgttac gcatgacgac gctcaactgc agcggacaat gccatttaca
    31801 agccgtggag gcgaagaaat gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta
    31861 tttctgccgt tgaagtctgc gcttgccctg aactgctact ttggttcatc cggtggctcg
    31921 gtagaaaaat cagaagcgat tcaacctttt gctgtaccag gcaatgccaa actcggcgat
    31981 aagatttggg aatctgacga tattaaaatt cccgtctatt gtgacaacaa taccaacggc
    32041 aattttgaaa gtgagcatgt ttatgcctgg gtaaacccct acccgggggt gcaggatcgc
    32101 tattatcaac tcggcgtaac ttacaatggc gttgattatg acgccaacca gggaaaaagc
    32161 cgcatcgaca ctaaccagtg tattgacagt aagaacattg acatttatac ccctgagcag
    32221 attatcgcga tgggatggca gaacaaaatt tgctcgggtg atcccgctaa tattcacatg
    32281 tcgcgtacct ttctcgctcg catgcggtta tacgtcaaaa tacgagaaat gccgccgcat
    32341 gattatcaaa gtacgcttag cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt
    32401 aacgaagacc ctactgccca aaacctgaaa tatcatatta ctggtctgga aaacattcgc
    32461 gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa cacaggtgat tgattttggt
    32521 aaatttaatt tgctggatat tcgtcgccac acaatgtcga aaacattcag tattaagacg
    32581 acaaaatcac aaaatgacca atgcaccgac ggatttaaag tcagttcctc cttttatacc
    32641 gaagaaacgt tggttgaaga agataaagca ttgttgattg gtaatggtct gaagctacgt
    32701 ttgctggatg aaaacgcctc gccttatacc ttcaataaat atgctgaata cgccgatttc
    32761 accagcgaca tgttggtcta cgaaaaaacc tatacagctg aactttcgtc cattgcaggt
    32821 acccccatcg aggctgggcc cttcgataca gtggtgcttt ttaaaattaa ctataactga
    32881 tttgccatga tgccctttgc agccggaaag ggcatctctt tcagaaaatt gctgattttc
    32941 cccttttcct tgattgctgt tattttttcc tggcccggct ttctttatgc tgtatcgata
    33001 caccaaagag aaaataatga gggagcgaag gatgccagtt ttgcagtggg gaatgttatg
    33061 tgtgctatca cttctccttt ctattggttt cctcgcggta catctcccgg cggcgctatt
    33121 acttgggccg atgatcgccg ggatcatctt cagtatgcgc ggaattactc tgcaacttcc
    33181 ccgctccgct tttctcgccg cgcaggccat tcttggctgc atgattgcgc aaaacctcac
    33241 tggttcgatt ctcaccacgc tggccgccaa ctggccgatc gtgcttgcta ttttactggt
    33301 gacgctgctt tccagcgcca tcgtgggttg gttattggtg cgctatagct cactgcctgg
    33361 aaataccggt gcctggggtt cttctccagg cggcgcagcg gcaatggtcg ctatggcgca
    33421 agattacggc gcagatattc gcctggttgc atttatgcaa tatctgcgag tgctgttcgt
    33481 cgcgggggcc gctgttctgg tcacccgcat gatgctgggt gataacgctg aagcggttaa
    33541 ccagcaaatc gtctggttcc cgccagtgag cattaatctc ctgcttacga ttttgctcgc
    33601 agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct tccggcacga tgctcatccc
    33661 gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc accatcgaac taccggaatg
    33721 gctgctggcg atggcgtata tggcaattgg ctggcggatt ggtcttggtt tcgataaaca
    33781 aatcttactg cgggcactac gcccgctacc gcaaatcctg ctgtcgattt ttgctctgct
    33841 ggctatttgt gcgggtatgg cgtgggggct gacccggttt atgcacattg attttatgac
    33901 tgcctacctc gccaccagtc ccggcgggct tgataccgtg gcggtcatcg ccgcagggag
    33961 caatgccgat atggcgctta ttatggcgat gcaaaccctg cgcctgttca gtattttgct
    34021 gacggggcca gccattgcgc ggtttatttc aacctatgcg ccgaagcggt cggcctagtg
    34081 ctggcagcca gggcaccagt aaaacgggcg agatgacagc gtggttttct caatgatgct
    34141 gccacagcgt tcgcacggtt cgccatcacg atgaaaaacc ttaaagcgaa acagcgcccc
    34201 atgatgctta ttctcatcca ccagccccct cgtagcgtag gaaaatcgag gaatatccag
    34261 taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga tctttcgctt tatgatttcc
    34321 agtcaaccca acctgccaga ggatctccac ccgcaaataa ttgccaagcc cagccagaaa
    34381 cgcctgatcg agcagtaatc ctgcaaactg acggttacga aagcgcggtg acaataaccg
    34441 ttcctttacc acctccggcg tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa
    34501 aaacggatgc gtggtcagtt gttccggcct caacatctca atatccgagg cgctataaag
    34561 cagaatggtt ttgtcagccg tttgcagttt tacccgcaat actcgcgtgg tctgcggctc
    34621 ttcgccggta tcaaccacgc gccagacgcc gtaaagctga ttatggctgt agagcgttaa
    34681 gtcgttggaa aaatgagtta acaacgcctt accacgcgtt tccacatggg taacgtgttg
    34741 accgataagt tgtgattgat aagtttttaa ctgcgggaag gcaaaccaga catcagttag
    34801 tggtttgcct ttgatcgccg cctccaggtt atccgctgca cggcggatct ccgggccttc
    34861 aggcatggtg atatcctttt ttcattcttt attaatcggg gttatgctgc gacaacaatc
    34921 tccttttcgg caaatgtaga gcgtagtcgg cgggcgaacg ccagtgcgtg ctcgccgtcg
    34981 ccatgcaggc agactgtttg cgccgtgacc gttgcccatt cgccggtgat acttttgact
    35041 ctgccgtgtt gcaccatctc tagcgtttgc gccaacgcct gttcttcgtt ttcaatcaac
    35101 gcgcctcgct gacttcgcgg caccagcgag ccgtcagcct gataaccgcg atcggcaaac
    35161 acttcctcgc gcgttgtcag gccatattgc ttgcctgcac gaatcagctc gcttcctgcc
    35221 agcccaacga gaatcaatgc cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc
    35281 agttgtgctt ctttcgccgc ctggttgtac aacatgccgt gcggtttgac atgacgcatt
    35341 acgccgcctt gcgcacgggc aatcgtttcc agcgcgccaa tttgatacag cgtctgggcg
    35401 taaacggttt ctggcggcag ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa
    35461 ctcgggtgag cgccaatcgc gacaccattt tttattgctt cacgcacgca agcctgcatg
    35521 atttgcgcat cgcctgcatg aaatccacag gcaatattgg cagaggaaac cagcgttaat
    35581 agctctgcgt cgctggcgca gccttcgccc agatcggcgt tcaggtcaat tttcattgtg
    35641 cagccgccac actaattgtt cgaaataacg ttgctgatct tgccgcgctt tcagtgcctc
    35701 ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc ggaatttgcg ccagatggta
    35761 catatcagcc tcaatgatac aggcaatacg cgggtaacca tcggtggtct gtgcgtcgtt
    35821 catcaacaca atcggctgcc cgttatgtgg cacctgcacc acgcccggta acaaaccgtg
    35881 agataacagt tcgcgatcgg tggtgcgttt taaaatttgc ccctgtaagc gatagcccat
    35941 gcggttactt tgcgagctaa gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg
    36001 atcgaactca tgatattccg gccccggcaa ggcgcgaatg cggttgcccc acagcagctg
    36061 tttaacgccc tgcgcttcca taaaatcacg cttcgatttg ccaatcggga gtcggtcacc
    36121 atccttcagt aaacggcctt ccagcccgcc aatccccact ttgagatcgg tgctgcatga
    36181 ccccattacc ggcggaacat caataccacc cgcgaccgcc agataactgc gcatcccgtg
    36241 ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc ggcaatcgcc agccggtcca
    36301 gacggcatta tcatccagcc gcgcttcgca accggcaccc gtcagagcaa accacccatc
    36361 agtttcgaac tcaaccgtta actgaccgag cgtgatctcc agcgcggggg cattggcgtc
    36421 attacccacc agcaggttag caatgcgtaa cgcaggcata tccagtgcgc cgcagtggct
    36481 gattcccgac tggcgaaaac cgtgacgacc gccatcctgc acagtggtat acatgcccgc
    36541 acgaataatc ttcagcatac tccctccttc tgcggtacaa agcgcacgct gtctcccgga
    36601 cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc
    36661 aactgccagc cacccggcgt tgccagcgga taaacaccag tctgcggccc gccgatcccg
    36721 acagaacctg ccggaacgag caagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc
    36781 ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta aaaaccagac cacgtattcc
    36841 acggaggagt gcaattcaac aacctgtttt tcgctaaacc cgcaatgcgc cgcgaccacc
    36901 gccaaatccg gtccgcctgc accaccgtaa accaccggaa tttcaataaa gcgagactca
    36961 ggttccaggg cctcgctctc ctcccaccag cgttgcaaac gctctatggc atccagcgcc
    37021 agcgactcag gattacgcaa aatcaccgtg atattgttca tgccgggaat ggcttcaacc
    37081 acattcggca tatccaccag acgctgcgcc agtcgccaga tccgtttctg gctagccagc
    37141 gtcaccggcg gttccagttc cagcactacc gccgtttcac ctatcagata acaacgcgct
    37201 cgttgcactt tcgatccctc tgattattcg ttatgcagga ttaggaatat caataaaggt
    37261 cacatcaaga tcggtatttt cattcagcca ctcgctcaat gcgcgaatac caccacgttc
    37321 agtggcatgg tgacctgcag cataaaaatg caacccctgc tcgcgggctg aatgaatggt
    37381 ctgttcagaa acttcgccag taataaaagc atccacgcca aaacgcgcgg cgctatcgat
    37441 aaaactttgc ccgccgcccg tgcaccaggc gacgcgctgg accacctcag gtccggtatc
    37501 gccacaccat aacggcttac gtcccagacg cgcttcaatc caggaggcta gttccagtcc
    37561 cggcacaggc atggtcagct ctccccacgg caccaacggc tcaatttcgc ccatgaccgt
    37621 gatccccagt aacgccgcca gctgtgcgtt attgcccagc tcaggatgta cgtcaagcgg
    37681 caaatgccag ccatacaggt tgatatcatt cgccagcaac gtttttaaac ggttacgctt
    37741 cattccgcga atgaccggag actctccttt ccagaagtag ccatgatgca caatgaccgc
    37801 atccgcgccc agacgcactg cctcatcgag caaagcctgg ctggcggtga caccggtaac
    37861 aattttttgc accgtctctt tgccttctac ctgcaaacca ttcggcgcgt aatcactaat
    37921 cgccgcgctg ttcagttttt cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc
    37981 gccttccgta ttctgatagc tacaccgtta acatagcgtt acctcgcctg ttcgtcgata
    38041 gccaatcttt tgacatatta agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat
    38101 catcacgctt cagctaatgt atatttatgc aatcagacag catattgcat aaataatcag
    38161 atctgtatca aagttttaac tttaaggaaa cacaagcacg ccgcgcaact attacgtgac
    38221 gttgcagtca attgtggaat tattatgaat aaacacgcat cacaaccgcg cactatttac
    38281 tatgtcgttg cgctgcaaat ctgggaatat tttagctttt acggcatgcg tgccctgctg
    38341 attctctatc tcaccaatca actaaaatac aacgatactc acgcctacga gttatttagc
    38401 gcctactgtt cgctggtgta tgtcacgcca atcctcggtg gctttttggc ggataaagtt
    38461 ctcggcaatc gcatggcggt gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg
    38521 ctgggtgcca gtgagatcca tccgtcattc ctctatctgt ccctggcgat tatcgtttgt
    38581 ggctatggtc tgtttaaatc caatgtcagt tgcctgctcg gcgagctgta tgagccaacc
    38641 gatccgcgtc gtgatggcgg tttctcgctg atgtatgcag cgggtaacgt ggggtctatt
    38701 atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca gctgggcgat gggctttggc
    38761 ctggcggcgg tgggtatgat cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc
    38821 acacataccc gcggcgttaa taaaaaagta ctgcgtgcaa caaactttct cctgcctaac
    38881 tggggatggc tgctggttct gctggtggca acgcctgcgc tgattaccgt actgttctgg
    38941 aaagagtggt cggtatacgc cttaattgtc gcgactatta ttggcctggg tgtactggca
    39001 aaaatttatc gcaaagcaga aaaccagaaa cagcgtaagg agctggggct gattgtgacg
    39061 ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac agggcggcag ctcaattagc
    39121 ctttatatcg accgcttcgt taaccgcgat atgtttggtt ataccgttcc gactgctatg
    39181 ttccagtcga ttaatgcctt cgcagttatg ctatgcggtg tgttcctggc gtgggtggtt
    39241 aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg gaaaatttgc tcttggcctt
    39301 ggcctgatga gcgccggatt ctgcattctg accttaagcg cccgctggtc cgcaatgtat
    39361 ggtcactctt ctctgccact gatggtgtta ggcctcgcgg tgatgggctt tgcggaactg
    39421 tttatcgacc cggttgccat gtcgcaaatt acgcgcattg aaattccggg cgtgaccggc
    39481 gtattaaccg gcatctacat gctgctttct ggcgcgatcg cgaactatct ggcgggcgtg
    39541 attgccgatc agacatcgca ggcttcgttt gatgcttccg gggcgatcaa ctactccatc
    39601 aatgcatata ttgaagtatt tgatcaaatt acctggggcg cactggcgtg tgtaggactg
    39661 gtactgatga tttggctgta tcaggcgctg aaattcagaa accgcgcgct ggcgctggag
    39721 tcataatagg ccagcaccga tgcctgatgc gacgcttgac gcgtcttatc a
//
LOCUS       DAJVYM010000019        39477 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVYM010000019 DAJVYM010000000
VERSION     DAJVYM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.019"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQS60_000948"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQS60_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS2386767.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OQS60_000949"
     CDS             1946..2230
                     /locus_tag="OQS60_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS2386768.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OQS60_000950"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OQS60_000950"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS2386769.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OQS60_000951"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OQS60_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS2386770.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OQS60_000952"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OQS60_000952"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS2386771.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="OQS60_000953"
     CDS             7236..7799
                     /locus_tag="OQS60_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2386772.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="OQS60_000954"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="OQS60_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS2386773.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="OQS60_000955"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="OQS60_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="OQS60_000956"
     CDS             11269..11817
                     /locus_tag="OQS60_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2386774.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="OQS60_000957"
     CDS             11814..12284
                     /locus_tag="OQS60_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2386775.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="OQS60_000958"
     CDS             12281..12805
                     /locus_tag="OQS60_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2386776.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="OQS60_000959"
     CDS             12792..13664
                     /locus_tag="OQS60_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS2386777.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OQS60_000960"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OQS60_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS2386778.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="OQS60_000961"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="OQS60_000961"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS2386779.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="OQS60_000962"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="OQS60_000962"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS2386780.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="OQS60_000963"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="OQS60_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS2386781.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="OQS60_000964"
     CDS             17383..18192
                     /locus_tag="OQS60_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS2386782.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="OQS60_000965"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="OQS60_000965"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS2386783.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="OQS60_000966"
     CDS             18774..19052
                     /locus_tag="OQS60_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS2386784.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OQS60_000967"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OQS60_000967"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS2386785.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="OQS60_000968"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="OQS60_000968"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS2386786.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="OQS60_000969"
     CDS             22833..24011
                     /locus_tag="OQS60_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2386787.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OQS60_000970"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OQS60_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS2386788.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="OQS60_000971"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="OQS60_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS2386789.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="OQS60_000972"
     CDS             26304..27317
                     /locus_tag="OQS60_000972"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS2386790.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="OQS60_000973"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="OQS60_000973"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS2386791.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="OQS60_000974"
     CDS             28212..28871
                     /locus_tag="OQS60_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2386792.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="OQS60_000975"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="OQS60_000975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS2386793.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="OQS60_000976"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="OQS60_000976"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS2386794.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="OQS60_000977"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="OQS60_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS2386795.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="OQS60_000978"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="OQS60_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS2386796.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="OQS60_000979"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="OQS60_000979"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS2386797.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="OQS60_000980"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="OQS60_000980"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS2386798.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..35944
                     /gene="argT"
                     /locus_tag="OQS60_000981"
     CDS             35162..35944
                     /gene="argT"
                     /locus_tag="OQS60_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS2386799.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36165..36947
                     /gene="hisJ"
                     /locus_tag="OQS60_000982"
     CDS             36165..36947
                     /gene="hisJ"
                     /locus_tag="OQS60_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS2386800.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37037..37723
                     /gene="hisQ"
                     /locus_tag="OQS60_000983"
     CDS             37037..37723
                     /gene="hisQ"
                     /locus_tag="OQS60_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS2386801.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37720..38436
                     /locus_tag="OQS60_000984"
     CDS             37720..38436
                     /locus_tag="OQS60_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2386802.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38444..39217
                     /gene="hisP"
                     /locus_tag="OQS60_000985"
     CDS             38444..39217
                     /gene="hisP"
                     /locus_tag="OQS60_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS2386803.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9348 a         9672 c        10614 g         9843 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acgggactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatactg caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    35641 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc
    35701 gttacaagat gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt
    35761 cgcctttgct ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    35821 gctacgtaaa gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg
    35881 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    35941 ctgatacgtc gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt
    36001 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    36061 tctaacgggg cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa
    36121 acctgtaaat attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc
    36181 tatcgctctc tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc
    36241 aaaacatccg catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag
    36301 gcgaactggt tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc
    36361 aatgtacgtt tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga
    36421 ttgacgccat catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca
    36481 ccgacaaact gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc
    36541 cgacagtcga gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga
    36601 cgttcggtaa tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg
    36661 acaacattta ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg
    36721 ctgccagcga aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt
    36781 ctgttaaaga tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata
    36841 acgaactgcg cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg
    36901 agaaattagc gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga
    36961 aagaaagccc gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca
    37021 cgacaggaca ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt
    37081 cacgctggag ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc
    37141 tggcggtaag ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct
    37201 gattcgtggc gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc
    37261 gctaaacacg gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc
    37321 tggtattatc actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc
    37381 ttttatggca gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg
    37441 gcaagtgttt cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg
    37501 caacaactgg caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga
    37561 tgtggtcaaa gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat
    37621 cgtctgtggc gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct
    37681 tgagcgccgc tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa
    37741 tactggaaac cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg
    37801 tggctgctta ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt
    37861 cgtgtctcca gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc
    37921 ggtacgccgc tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt
    37981 gttaagggaa ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg
    38041 gcgctgacgc ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg
    38101 gttccgcatg gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat
    38161 cgttgcatta ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg
    38221 atcctgatgc tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa
    38281 atagcccgcg atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg
    38341 gtgctctatt taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc
    38401 tggttgcagc atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt
    38461 aaacgttatc gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact
    38521 gcaagcgaat gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac
    38581 ctttctgcgc tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg
    38641 ccagacgatc aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca
    38701 actgcgctta ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca
    38761 tatgacggtg ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca
    38821 ggaagcgcgc gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca
    38881 ggggaaatat ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc
    38941 gctggcgatg gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga
    39001 actggtaggc gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt
    39061 ggtagtgact cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca
    39121 tcaggggaaa atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc
    39181 tcgtctgcaa cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct
    39241 gccggagtat tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag
    39301 tccgctaaaa atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt
    39361 gttaatcgtg gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa
    39421 tatcgacaac ttccacgccg catgatgcgg tatttaaatc ttttttacgc catccag
//
LOCUS       DAJVYM010000020        39409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVYM010000020 DAJVYM010000000
VERSION     DAJVYM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.020"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..266
                     /locus_tag="OQS60_000986"
     CDS             <1..266
                     /locus_tag="OQS60_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS2386804.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OQS60_000987"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OQS60_000987"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS2386805.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQS60_000988"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQS60_000988"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS2386806.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OQS60_000989"
     CDS             complement(1570..3270)
                     /locus_tag="OQS60_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS2386807.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OQS60_000990"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OQS60_000990"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS2386808.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OQS60_000991"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OQS60_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS2386809.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OQS60_000992"
     CDS             4828..6600
                     /locus_tag="OQS60_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS2386810.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OQS60_000993"
     CDS             6593..8374
                     /locus_tag="OQS60_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS2386811.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OQS60_000994"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OQS60_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS2386812.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OQS60_000995"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OQS60_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS2386813.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQS60_000996"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQS60_000996"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS2386814.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OQS60_000997"
     CDS             11336..11908
                     /locus_tag="OQS60_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS2386815.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OQS60_000998"
     CDS             complement(11939..12250)
                     /locus_tag="OQS60_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS2386816.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OQS60_000999"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQS60_000999"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OQS60_001000"
     CDS             12629..12982
                     /locus_tag="OQS60_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS2386817.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQS60_001001"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQS60_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OQS60_001002"
     CDS             complement(14736..15206)
                     /locus_tag="OQS60_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS2386818.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQS60_001003"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQS60_001003"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS2386819.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQS60_001004"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQS60_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS2386820.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQS60_001005"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQS60_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS2386821.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OQS60_001006"
     CDS             complement(17208..20357)
                     /locus_tag="OQS60_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS2386822.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQS60_001007"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQS60_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS2386823.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OQS60_001008"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OQS60_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS2386824.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OQS60_001009"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OQS60_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS2386825.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQS60_001010"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQS60_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS2386826.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQS60_001011"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQS60_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS2386827.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OQS60_001012"
     CDS             26836..27213
                     /locus_tag="OQS60_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS2386828.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OQS60_001013"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OQS60_001013"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS2386829.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OQS60_001014"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OQS60_001014"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS2386830.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OQS60_001014"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OQS60_001015"
     CDS             30024..30353
                     /locus_tag="OQS60_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS2386831.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OQS60_001016"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OQS60_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS2386832.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OQS60_001017"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OQS60_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS2386833.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OQS60_001018"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OQS60_001018"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS2386834.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OQS60_001019"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OQS60_001019"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS2386835.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQS60_001020"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQS60_001020"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS2386836.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRIFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OQS60_001021"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OQS60_001021"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS2386837.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OQS60_001022"
     CDS             complement(37406..39082)
                     /locus_tag="OQS60_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS2386838.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9527 a        10050 c        10416 g         9416 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccagaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag atacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAJVYM010000021        39259 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVYM010000021 DAJVYM010000000
VERSION     DAJVYM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.021"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQS60_001023"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQS60_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS2386839.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQS60_001024"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQS60_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS2386840.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OQS60_001025"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OQS60_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS2386841.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OQS60_001026"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OQS60_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS2386842.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OQS60_001027"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OQS60_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS2386843.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OQS60_001028"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OQS60_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS2386844.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OQS60_001029"
     CDS             complement(6574..7338)
                     /locus_tag="OQS60_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2386845.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQS60_001030"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQS60_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS2386846.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OQS60_001031"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OQS60_001031"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS2386847.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OQS60_001032"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OQS60_001032"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS2386848.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OQS60_001033"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OQS60_001033"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS2386849.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OQS60_001034"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OQS60_001034"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS2386850.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OQS60_001035"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OQS60_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS2386851.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OQS60_001036"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OQS60_001036"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS2386852.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQS60_001037"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQS60_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS2386853.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OQS60_001038"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OQS60_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS2386854.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OQS60_001039"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OQS60_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS2386855.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OQS60_001040"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OQS60_001040"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS2386856.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OQS60_001041"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OQS60_001041"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS2386857.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OQS60_001042"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OQS60_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS2386858.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OQS60_001043"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OQS60_001043"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS2386859.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQS60_001044"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQS60_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS2386860.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQS60_001045"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQS60_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS2386861.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQS60_001046"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQS60_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS2386862.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OQS60_001047"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OQS60_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQS60_001048"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQS60_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS2386863.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OQS60_001049"
     CDS             complement(31717..33003)
                     /locus_tag="OQS60_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2386864.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OQS60_001050"
     CDS             complement(33054..33995)
                     /locus_tag="OQS60_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS2386865.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OQS60_001051"
     CDS             complement(34010..34780)
                     /locus_tag="OQS60_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS2386866.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="OQS60_001052"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="OQS60_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS2386867.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="OQS60_001053"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="OQS60_001053"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS2386868.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39259
                     /gene="caiB"
                     /locus_tag="OQS60_001054"
                     /pseudo
     CDS             38069..39259
                     /gene="caiB"
                     /locus_tag="OQS60_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9395 a        10792 c        10279 g         8793 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aacttgaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaatgca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc gattttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcagc
//
LOCUS       DAJVYM010000022        39111 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVYM010000022 DAJVYM010000000
VERSION     DAJVYM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.022"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OQS60_001055"
     CDS             complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OQS60_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS2386869.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            574..2772
                     /gene="priA"
                     /locus_tag="OQS60_001056"
     CDS             574..2772
                     /gene="priA"
                     /locus_tag="OQS60_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS2386870.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2928..3953
                     /gene="cytR"
                     /locus_tag="OQS60_001057"
     CDS             2928..3953
                     /gene="cytR"
                     /locus_tag="OQS60_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS2386871.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4045..5004
                     /gene="ftsN"
                     /locus_tag="OQS60_001058"
     CDS             4045..5004
                     /gene="ftsN"
                     /locus_tag="OQS60_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS2386872.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5097..5627
                     /gene="hslV"
                     /locus_tag="OQS60_001059"
     CDS             5097..5627
                     /gene="hslV"
                     /locus_tag="OQS60_001059"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS2386873.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5637..6968
                     /gene="hslU"
                     /locus_tag="OQS60_001060"
     CDS             5637..6968
                     /gene="hslU"
                     /locus_tag="OQS60_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS2386874.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7035..7961
                     /gene="menA"
                     /locus_tag="OQS60_001061"
     CDS             7035..7961
                     /gene="menA"
                     /locus_tag="OQS60_001061"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS2386875.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8054..8539
                     /gene="rraA"
                     /locus_tag="OQS60_001062"
     CDS             8054..8539
                     /gene="rraA"
                     /locus_tag="OQS60_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS2386876.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OQS60_001063"
     CDS             complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OQS60_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS2386877.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9295..10140
                     /gene="glpF"
                     /locus_tag="OQS60_001064"
     CDS             9295..10140
                     /gene="glpF"
                     /locus_tag="OQS60_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS2386878.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10163..11674
                     /gene="glpK"
                     /locus_tag="OQS60_001065"
     CDS             10163..11674
                     /gene="glpK"
                     /locus_tag="OQS60_001065"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS2386879.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="OQS60_001066"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="OQS60_001066"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS2386880.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="OQS60_001067"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="OQS60_001067"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS2386881.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OQS60_001068"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OQS60_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS2386882.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /locus_tag="OQS60_001069"
     CDS             complement(14117..14416)
                     /locus_tag="OQS60_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS2386883.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /locus_tag="OQS60_001070"
     CDS             complement(14628..15068)
                     /locus_tag="OQS60_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS2386884.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /locus_tag="OQS60_001071"
     CDS             15169..15768
                     /locus_tag="OQS60_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS2386885.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="OQS60_001072"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="OQS60_001072"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS2386886.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OQS60_001073"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OQS60_001073"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS2386887.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OQS60_001074"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OQS60_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS2386888.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OQS60_001075"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OQS60_001075"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS2386889.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OQS60_001076"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OQS60_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS2386890.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OQS60_001077"
     CDS             complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OQS60_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS2386891.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21712..22410
                     /gene="cpxR"
                     /locus_tag="OQS60_001078"
     CDS             21712..22410
                     /gene="cpxR"
                     /locus_tag="OQS60_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS2386892.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22407..23780
                     /gene="cpxA"
                     /locus_tag="OQS60_001079"
     CDS             22407..23780
                     /gene="cpxA"
                     /locus_tag="OQS60_001079"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS2386893.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OQS60_001080"
     CDS             complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OQS60_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS2386894.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OQS60_001081"
     CDS             complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OQS60_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS2386895.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OQS60_001082"
     CDS             complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OQS60_001082"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS2386896.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26857..27891
                     /gene="rhaT"
                     /locus_tag="OQS60_001083"
     CDS             26857..27891
                     /gene="rhaT"
                     /locus_tag="OQS60_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS2386897.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OQS60_001084"
                     /pseudo
     CDS             complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OQS60_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OQS60_001085"
     CDS             complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OQS60_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS2386898.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29934..31403
                     /gene="rhaB"
                     /locus_tag="OQS60_001086"
     CDS             29934..31403
                     /gene="rhaB"
                     /locus_tag="OQS60_001086"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS2386899.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31400..32659
                     /gene="rhaA"
                     /locus_tag="OQS60_001087"
     CDS             31400..32659
                     /gene="rhaA"
                     /locus_tag="OQS60_001087"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS2386900.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32901..33725
                     /gene="rhaD"
                     /locus_tag="OQS60_001088"
     CDS             32901..33725
                     /gene="rhaD"
                     /locus_tag="OQS60_001088"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS2386901.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33735..34049
                     /gene="rhaM"
                     /locus_tag="OQS60_001089"
     CDS             33735..34049
                     /gene="rhaM"
                     /locus_tag="OQS60_001089"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS2386902.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34350..34796
                     /locus_tag="OQS60_001090"
     CDS             34350..34796
                     /locus_tag="OQS60_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS2386903.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34807..36258
                     /locus_tag="OQS60_001091"
     CDS             34807..36258
                     /locus_tag="OQS60_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS2386904.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36248..37318
                     /locus_tag="OQS60_001092"
     CDS             36248..37318
                     /locus_tag="OQS60_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS2386905.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37318..39066
                     /locus_tag="OQS60_001093"
     CDS             37318..39066
                     /locus_tag="OQS60_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS2386906.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
BASE COUNT         9520 a        10143 c        10165 g         9283 t
ORIGIN      
        1 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
       61 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      121 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      181 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      241 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      301 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      361 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      421 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      481 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      541 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
      601 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatggcggt taaagccggg
      661 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtatcagtt
      721 agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
      781 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
      841 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      901 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggccaggc ggtggatctg
      961 aacagcctga aacgctcacc caagcaacaa caggcgctgg cggcgttacg gcaagggaaa
     1021 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca gacgctgcgc
     1081 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1141 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aggccaccgc cgttggcgca
     1201 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1261 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1321 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacggttt
     1381 aatgcgcccg tggaagttct gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg
     1441 ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1501 ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc acgacagttc ctacaagcag
     1561 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1621 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag
     1681 aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa
     1741 cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1801 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa ccgccgtggc
     1861 tttgcgcctg cgctactgtg ccacgactgc ggctggattg ccgaatgccc acgttgcgat
     1921 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     1981 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtacc cgtggggctg
     2041 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt
     2101 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtacat
     2161 cgcggcggtg cgaggatttt gatcggcaca caaatgctgg cgaaaggtca ccactttccg
     2221 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2281 tcggcagagc gtttcgctca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2341 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2401 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2461 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2521 ccgctgttcc tgcaacaact gcgtaatctg atcctctcca gcccgctggc agacgacaaa
     2581 ttgtgggttc tcggtccggt tccggcactg gcacctaaac gtggcggtcg ctggcgctgg
     2641 cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattaa cggtacgctg
     2701 gcactcatca atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgttgatccg
     2761 attgagggtt aaaccgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     2821 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2881 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     2941 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3001 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3061 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3121 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3181 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3241 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3301 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3361 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3421 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3481 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3541 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3601 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3661 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3721 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3781 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3841 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3901 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     3961 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4021 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4081 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4141 tctcccgcta tggtcgctat tgctgccgcc gtgcttgtga cctttatcgg tggtctgtac
     4201 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4261 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4321 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4381 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4441 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4501 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4561 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4621 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4681 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4741 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4801 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4861 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4921 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     4981 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5041 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5101 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5161 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5221 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5281 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcaaaag
     5341 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5401 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5461 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
     5521 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5581 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5641 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5701 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5761 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5821 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5881 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
     5941 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6001 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6061 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6121 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6181 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6241 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6301 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6361 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6421 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6481 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6541 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
     6601 tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6661 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6721 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6781 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6841 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6901 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     6961 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7021 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7081 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7141 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggt actaattacc gccgggctat
     7201 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7261 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7321 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggctggca ctggttgcag
     7381 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7441 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7501 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
     7561 atacattgat tccggcactg atccttccgg cgaccgcctg tggcctgctg gcaacggcag
     7621 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7681 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7741 ctctggtatg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7801 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7861 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7921 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     7981 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
     8041 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8101 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8161 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8221 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8281 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggttattt acggcgcggt
     8341 gcgtcaggta gatgacctag aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8401 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8461 aaccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga
     8521 tccgctggat attgaatgat aagaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8581 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
     8641 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8701 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8761 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8821 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8881 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
     8941 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
     9001 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacat
     9061 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
     9121 atcagatgga ataaattgcg cgataacgct cattttatga cgaagcacac acattttaag
     9181 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
     9241 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
     9301 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
     9361 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
     9421 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
     9481 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
     9541 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
     9601 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
     9661 cgcggcagtg ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat
     9721 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
     9781 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
     9841 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
     9901 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
     9961 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    10021 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    10081 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    10141 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    10201 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    10261 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    10321 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    10381 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    10441 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    10501 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    10561 tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    10621 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    10681 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    10741 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    10801 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    10861 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg
    10921 tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct
    10981 gatgaacacc ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    11041 cggcccgact ggcgaagtga actatgcgct ggaaggtgcg gtgtttatgg caggcgcatc
    11101 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    11161 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    11221 tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa
    11281 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    11341 ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc
    11401 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg
    11461 cccggaagtt cgcgaagtga ctgcattggg cgcggcctat cttgctggtc tggcggtcgg
    11521 tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc
    11581 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggttaaacg
    11641 cgcgatggcg tgggaagaac acgacgtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaatatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgatcgtcgg
    12181 accgggagcc aaaggcacca ttgatttgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acaattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcaaca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tctatccact atctggatcg
    12781 caaggacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgactggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gaggaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac agcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccacgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcta ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccacg ttccgcaatc gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    18961 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccactatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaaccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    21061 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    21121 gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    21181 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    21241 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    21301 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    21361 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    21421 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    21481 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    21541 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    21601 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    21661 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    21721 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    21781 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    21841 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    21901 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    21961 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    22021 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    22081 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    22141 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    22201 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    22261 gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac
    22321 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    22381 cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    22441 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    22501 cacgccagat gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc
    22561 atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    22621 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    22681 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    22741 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    22801 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    22861 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    22921 tgttggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    22981 agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    23041 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    23101 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    23161 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    23221 aggaactcga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    23281 tggtgatgtc acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc
    23341 agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt
    23401 tgacagttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    23461 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    23521 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    23581 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc
    23641 gtgaatctgg cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc
    23701 gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    23761 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    23821 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    23881 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    23941 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24001 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24061 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24121 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24181 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24241 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24301 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24361 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24421 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24481 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24541 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24601 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24661 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24721 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    24781 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    24841 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    24901 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    24961 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25021 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25081 tggaatcagc gtttgcactg ctttgctgaa aaattctcgt aattcagggt caagattccc
    25141 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25201 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25261 caacacaaat gccccagctt cttctttcgt accgtactgc tgcatgatgg aagcgtaaag
    25321 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25381 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25441 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    25501 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25561 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25621 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25681 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg taattcctca
    25741 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    25801 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    25861 ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg atatgcggcc
    25921 tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa cgtgccgctg
    25981 cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg cggttctgga
    26041 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    26101 cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc accgccagtt
    26161 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    26221 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    26281 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    26341 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    26401 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    26461 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    26521 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    26581 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    26641 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    26701 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    26761 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    26821 agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    26881 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    26941 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    27001 ctgccgtgga ccatcagcgc cctgttacta ccggatttct gggcgtatta cagctcgttt
    27061 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    27121 aactatggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    27181 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg
    27241 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta
    27301 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    27361 ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    27421 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact tggcgtcgat
    27481 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcattaac
    27541 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa agccgacttc
    27601 tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg cgggttgatg
    27661 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    27721 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcgggctg
    27781 gtgctgaaag agtggaacaa tacaggacgc cgtccggtaa cggtgttgag cctcggttgt
    27841 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    27901 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    27961 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    28021 tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    28081 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    28141 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccaaccgggt aatcagctta
    28201 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    28261 agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc
    28321 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    28381 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgtcag
    28441 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    28501 acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg
    28561 cgataaggac gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    28621 caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact
    28681 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    28741 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    28801 agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg gtgaccagtt
    28861 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agcgataggc
    28921 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    28981 gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt
    29041 acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    29101 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    29161 caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc gactggcggt
    29221 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    29281 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg
    29341 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt
    29401 ggtcagacac agattatcgg tatgttcata cagatgccga tcatgagcgc gtacgaaaca
    29461 gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg
    29521 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    29581 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    29641 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc
    29701 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    29761 gaattttcag gaaatgcggt gagcatcaca tcaccacaat gaagctaatt gtgaacatca
    29821 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca
    29881 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    29941 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    30001 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc
    30061 atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg
    30121 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    30181 gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    30241 gcgatagccg ctccaatggc ctaatggcgc aggcacagca acaactcggc aaacgcgata
    30301 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gctttatcaa ctgcgtgcgc
    30361 tgacggagca acaacctgaa cttattccac acattgctca cgctctgctg atgccggatt
    30421 acttcagcta tcgcctgacc ggcaagatga actgggagta caccaacgcc acgaccacgc
    30481 aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggggcta
    30541 acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cattgggcac tggatttgcc
    30601 cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc agcgcggtta
    30661 tcgcctcgcc gttaaacggt tcacgcgctg cttatctctc ttctggcacc tggtcattga
    30721 tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc aacataacca
    30781 atgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctgc
    30841 ttcagcgagt gcttcaggag cggcaaatca acgatctccc ggcgcttatc gccgcgacac
    30901 aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt attaatcctg
    30961 aggcgatgtg cagcgaaatt caggctgcgt gtcgggaaat ggcgcaaccg ctcccggaaa
    31021 gtgatgctga actggcgcgc tgcattttcg acagtctggc gctgctgtat gccgatgtgt
    31081 tgcatgagct ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg
    31141 gctgccagaa cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg
    31201 ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg
    31261 aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct
    31321 ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagcttcac tcgacacgac
    31381 agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca
    31441 gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc
    31501 cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc
    31561 gctgaccggt gggattcagg ctattggtaa ttatccgggc aaagcgcgta atgccagtga
    31621 gctacgagcc gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt
    31681 acatgccatc tatctggaat cagacacgcc agtctcgcgc gaccagatca aacctgagca
    31741 cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc
    31801 ctgtttctcg catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat
    31861 tcgccagttc tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga
    31921 gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac
    31981 cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcga
    32041 gaagctggat ccggcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc
    32101 agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac
    32161 tgcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc
    32221 cgccgccatg ctgtatgtac cgcagttgct gctgcacgtc agccgtccgg ttcgctggga
    32281 cagcgatcac gtggtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg
    32341 tcacgatctg ttcgaccggg tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg
    32401 cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct
    32461 ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc
    32521 gctactggaa gaacagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg
    32581 tcacgatacg ccagcaggta gcgaatggct ggagagcgtg cgggcttatg agaaagcgat
    32641 tttgagccag cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacag
    32701 gtcgaatgac catgcgtgct gccaccctca ccccggccct ctccctgaaa gggcgaggga
    32761 gaaaaacata gcctcgtgcg gcaacggagc tgaacaccgc tccggacagt cccctcgccc
    32821 ctacggggag agggttaggg tgaggggaac aagccgcacc atgtctaaac aaggcaacct
    32881 aatttacagg aacacagaac atgcaaaaca ttacccagtc ctggtttgtc cagggaatga
    32941 tcaaagccac caccgatgcc tggctgaaag gctgggacga gcgcaatggt ggtaacctga
    33001 cgctgcgcct ggatgacgcc gatatcgcgc cttatcacgg caactttcat gcacaaccgc
    33061 gctatattcc cctcagccaa cccatgcctt tactggcaaa tacgccgttt attgtcaccg
    33121 gctccggcaa attcttccgt aacgtccagc ttgatcctgc agctaactta ggcgtcgtaa
    33181 aagtcgacag cgacggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc
    33241 caacttccga acttccggct cacttccttt cccactgcga gcgcattaaa gccaccaacg
    33301 gtaaagatcg ggtgatcatg cactgccacg ccaccaacct gatcgccctc acctatgtac
    33361 ttgaaaacga caccgcggtc tttactcgcc aactgtggga aggcagcacc gagtgtctgg
    33421 tggtattccc ggatggcgtt ggcattctgc cgtggatggt gcccggcacg gacgaaatcg
    33481 gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc ttccacggcg
    33541 tcttcggcag cgggtcgacg ttggatgaaa cattcggttt aatcgacacc gcagaaaaat
    33601 cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc atcagccgtg
    33661 aagagttgat agcgctcggc aagcgcttcg gcgttacacc actagctagt gcgctggcgc
    33721 tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggttaacccc gacgcccacg
    33781 aagagtatca gcgtcggcat aaccccatct ggccggaact ggaagcagtg ttgaaatctc
    33841 acggtgcgca taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccacgg
    33901 tagagattga atctgaagaa cggtggaatg cggtagccag cactgatgtt tgccaacgtt
    33961 ggtggaaata tatgaccgat gttatgcccg ctaacccgga taacagcccg gtgagtagcg
    34021 agctgcaaga agtgttttac ctgccgtaat tgcccaatgt gccagagatg cctccggcac
    34081 aatattttaa atttcgattt gaaaaattaa acatattaca tctcccggta agttatattt
    34141 ccctgataca ttgtgagtaa atcacaaaaa taatgaataa cccattaatg attcatgtgg
    34201 tttatttaaa taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa
    34261 attgtcattg tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg
    34321 attaaaatat attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc
    34381 tgaatattca gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac
    34441 aaaacggttt tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa
    34501 tgcactctac tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta
    34561 aacaaccgtt tgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg
    34621 gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg
    34681 tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc
    34741 aacagggcga cgccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa
    34801 gtggcgatgg agtcatcctt atgtattgtc gcgatcacca actgccccgc cgggatcgct
    34861 cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata
    34921 aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc
    34981 gctgccgatt acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg
    35041 tttgccggga agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag
    35101 attttcagcg aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt
    35161 ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg
    35221 gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag
    35281 ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa
    35341 tccatcggtt accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg
    35401 tcgattgccg ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac
    35461 aaagcgctgc tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg
    35521 gcgattggct atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg
    35581 ctgctcggtt caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac
    35641 tacgttatcg ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc
    35701 attcctccat caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat
    35761 atgggcggcc ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac
    35821 atttatgact ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt
    35881 cttgcgacgt tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt
    35941 gccattgtgg tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct
    36001 ccgttgccga tgattaccgc caatacgctg gcgggtggca ttaccggcgt gctggtgatc
    36061 gctttcggaa tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg
    36121 atgtcgccgg tgggttcgtt ttatctggtg ctagcgattg gcctcgcgct gaacatttca
    36181 ttcattatcg tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag
    36241 cttgtccatg aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga
    36301 tgaacaggaa gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac
    36361 ctttgatggt ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt
    36421 tgtcaggcat atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct
    36481 gcgctttacc accattggcg gctggtggaa tcagtcaatg ctcaaccacc gggtaaccat
    36541 acgcacacac aagggattta aaatccctgg tgtgattggt tccgtcgcac ctcatgcgtt
    36601 aacggaaaag caaaagcaac agccgctgtc atttgatgag atgttcattg atattggcgc
    36661 gaacagtcgc gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga
    36721 agccaatttt gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat
    36781 cggctgcgca atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta
    36841 tggcgtcggc agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca
    36901 cattaaaccg gatgtggtga ttgtgctgga taccgctgtc gcaggtgatg ttccgggcat
    36961 tgataacatt aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa
    37021 gcgctacttc cccaaccaga aactggtggc ggcgttaaaa agctgtgccg cacataacga
    37081 tttaccgctg caattttcca ccatgaaaac cggtgcgacg gatggcgggc gctacaacgt
    37141 gatgggcggc gggcgtccgg ttgttgcgct gtgcctgcca acccgctatc tgcacgctaa
    37201 cagcggcatg atttcaaaag ccgattacga tgctctgctc acgttgatac gggattttct
    37261 gaccacctta accgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg
    37321 ctaaacgaac gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggt
    37381 gagctggcgc aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc
    37441 aactttaccc ttaacggcaa agcccgcatt ttcgccagtg gcagtgcggg ctatcagctg
    37501 gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg
    37561 ctgctggcgc tgttattact gaataccttt actccccgtg cgcaactcgc ctcggcgctt
    37621 aatttgccag aaacctgggt agcagagcgt ctgccccggt taaaacagcg ttatgaacgc
    37681 acttgttgcc tggccagtcg ccccggtttg ggccatttca ttgatgagac agaagagaaa
    37741 cgcgttatct tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc
    37801 gtaacacgag acaattttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg
    37861 ctcatgcagg gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa
    37921 ctgaccgatg agtggccgca atatcccggt aacgagataa aacaaatcgt tgaacagagc
    37981 ggcatgtttc ttggtgataa cgctgtaaga accctgacag gtttgataga gaaacagcat
    38041 cagcaagcgc aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccaggc
    38101 atcgcgtcat tgaatattat tgatacgcag ctggttgaga atattaccgg gcatttatta
    38161 cgttgccttg ccgcaccggt gtggattgcc gagcaccgcc aaagcagcat gaataacctg
    38221 aaagccgcct ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa
    38281 cagctcgata ttcccctttt cgatagcgat ctgatcggtt tgtattttgc ctgtgcgctg
    38341 gaacggcatc aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc
    38401 actattaatc agctcgccat tgagcgcgat gttttacatt gtcgggtgat tattgcccgt
    38461 agcttaagcg agcttattgc cattcgcgaa gagattgagc cgttattgat cattaacaac
    38521 agccattatt tactggatga cgcggtaaat aattacatca ccgtaaaaaa tatcattacg
    38581 gctgccgata tcgaacaaat aaaacacttt ctggcgacgg catttattcg ccaacagccg
    38641 gaacgctttt tctctgcccc cggaagtttt cattattcga atgtacgcgg tgaaagctgg
    38701 caacatatta cccggcaaat ttgtgcgcaa ttagtggcac aacaccatat taccgccgat
    38761 gaagcgcaac ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc
    38821 gccatcccac attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc
    38881 gcccaaccag ttgaggtgaa taacgaagtc attaaccatg tcttgatcgc ctgcgccgcc
    38941 gccgatgcgc gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat
    39001 ccggcagaag ttattgccgg gttaacagga tatgaggcat ttatggagtt acttcacaag
    39061 gggtgaactg tgtcggatgc gccgcatccg acactgcatt ttcatcaaaa a
//
LOCUS       DAJVYM010000023        38327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVYM010000023 DAJVYM010000000
VERSION     DAJVYM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.023"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            59..1423
                     /locus_tag="OQS60_001094"
     CDS             59..1423
                     /locus_tag="OQS60_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2386907.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OQS60_001095"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OQS60_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS2386908.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="OQS60_001096"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="OQS60_001096"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS2386909.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="OQS60_001097"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="OQS60_001097"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS2386910.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OQS60_001098"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OQS60_001098"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS2386911.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="OQS60_001099"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="OQS60_001099"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS2386912.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="OQS60_001100"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="OQS60_001100"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS2386913.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="OQS60_001101"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="OQS60_001101"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS2386914.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="OQS60_001102"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="OQS60_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS2386915.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OQS60_001103"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OQS60_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS2386916.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OQS60_001104"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OQS60_001104"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS2386917.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OQS60_001105"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OQS60_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS2386918.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OQS60_001106"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OQS60_001106"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS2386919.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OQS60_001107"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OQS60_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS2386920.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OQS60_001108"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OQS60_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS2386921.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="OQS60_001109"
     CDS             13749..14288
                     /locus_tag="OQS60_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS2386922.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="OQS60_001110"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="OQS60_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS2386923.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="OQS60_001111"
     CDS             complement(15648..15995)
                     /locus_tag="OQS60_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS2386924.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OQS60_001112"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OQS60_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS2386925.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OQS60_001113"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OQS60_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS2386926.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OQS60_001114"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OQS60_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS2386927.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OQS60_001115"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OQS60_001115"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS2386928.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OQS60_001116"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OQS60_001116"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS2386929.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="OQS60_001117"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="OQS60_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS2386930.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OQS60_001118"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OQS60_001118"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS2386931.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OQS60_001119"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OQS60_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS2386932.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OQS60_001120"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OQS60_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS2386933.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OQS60_001121"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OQS60_001121"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS2386934.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OQS60_001122"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OQS60_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS2386935.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="OQS60_001123"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="OQS60_001123"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS2386936.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="OQS60_001124"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="OQS60_001124"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS2386937.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="OQS60_001125"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="OQS60_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS2386938.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OQS60_001126"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OQS60_001126"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS2386939.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="OQS60_001127"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="OQS60_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS2386940.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="OQS60_001128"
     CDS             complement(37465..37920)
                     /locus_tag="OQS60_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386941.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="OQS60_001129"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="OQS60_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9468 a        10312 c         9725 g         8822 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcaca gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcg
//
LOCUS       DAJVYM010000024        37285 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVYM010000024 DAJVYM010000000
VERSION     DAJVYM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.024"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="OQS60_001130"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="OQS60_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OQS60_001131"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OQS60_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS2386942.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OQS60_001132"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OQS60_001132"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS2386943.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="OQS60_001133"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="OQS60_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="OQS60_001134"
     CDS             complement(4104..4388)
                     /locus_tag="OQS60_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386944.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OQS60_001135"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OQS60_001135"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS2386945.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="OQS60_001136"
     CDS             complement(5289..6497)
                     /locus_tag="OQS60_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2386946.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDIRSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="OQS60_001137"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="OQS60_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS2386947.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="OQS60_001138"
     CDS             complement(7292..8977)
                     /locus_tag="OQS60_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS2386948.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="OQS60_001139"
     CDS             9247..9624
                     /locus_tag="OQS60_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS2386949.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OQS60_001140"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OQS60_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS2386950.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="OQS60_001141"
     CDS             10071..10358
                     /locus_tag="OQS60_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS2386951.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..11064
                     /gene="nfsA"
                     /locus_tag="OQS60_001142"
     CDS             10342..11064
                     /gene="nfsA"
                     /locus_tag="OQS60_001142"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS2386952.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11125..12027
                     /gene="rimK"
                     /locus_tag="OQS60_001143"
     CDS             11125..12027
                     /gene="rimK"
                     /locus_tag="OQS60_001143"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS2386953.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12115..12591
                     /locus_tag="OQS60_001144"
     CDS             12115..12591
                     /locus_tag="OQS60_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS2386954.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12942..14054
                     /gene="potF"
                     /locus_tag="OQS60_001145"
     CDS             12942..14054
                     /gene="potF"
                     /locus_tag="OQS60_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS2386955.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14149..15282
                     /gene="potG"
                     /locus_tag="OQS60_001146"
     CDS             14149..15282
                     /gene="potG"
                     /locus_tag="OQS60_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS2386956.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15292..16245
                     /gene="potH"
                     /locus_tag="OQS60_001147"
     CDS             15292..16245
                     /gene="potH"
                     /locus_tag="OQS60_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS2386957.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16242..17087
                     /gene="potI"
                     /locus_tag="OQS60_001148"
     CDS             16242..17087
                     /gene="potI"
                     /locus_tag="OQS60_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS2386958.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            17147..17635
                     /locus_tag="OQS60_001149"
     CDS             17147..17635
                     /locus_tag="OQS60_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS2386959.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17676..18803
                     /gene="rlmC"
                     /locus_tag="OQS60_001150"
     CDS             17676..18803
                     /gene="rlmC"
                     /locus_tag="OQS60_001150"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS2386960.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OQS60_001151"
     CDS             complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OQS60_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS2386961.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OQS60_001152"
     CDS             complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OQS60_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS2386962.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OQS60_001153"
     CDS             complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OQS60_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS2386963.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OQS60_001154"
     CDS             complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OQS60_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2386964.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OQS60_001155"
     CDS             complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OQS60_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS2386965.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23087..23602)
                     /locus_tag="OQS60_001156"
     CDS             complement(23087..23602)
                     /locus_tag="OQS60_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2386966.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24251..26020
                     /locus_tag="OQS60_001157"
     CDS             24251..26020
                     /locus_tag="OQS60_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS2386967.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            26231..26554
                     /locus_tag="OQS60_001158"
     CDS             26231..26554
                     /locus_tag="OQS60_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS2386968.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26551..27381
                     /gene="amiD"
                     /locus_tag="OQS60_001159"
     CDS             26551..27381
                     /gene="amiD"
                     /locus_tag="OQS60_001159"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS2386969.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(27378..28391)
                     /locus_tag="OQS60_001160"
     CDS             complement(27378..28391)
                     /locus_tag="OQS60_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS2386970.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28490..29920)
                     /locus_tag="OQS60_001161"
     CDS             complement(28490..29920)
                     /locus_tag="OQS60_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS2386971.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OQS60_001162"
     CDS             complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OQS60_001162"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS2386972.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OQS60_001163"
     CDS             complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OQS60_001163"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS2386973.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OQS60_001164"
     CDS             complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OQS60_001164"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS2386974.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OQS60_001165"
                     /gene_synonym="priS"
     CDS             complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OQS60_001165"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS2386975.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OQS60_001166"
     CDS             complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OQS60_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS2386976.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36990..>37285)
                     /locus_tag="OQS60_001167"
     CDS             complement(36990..>37285)
                     /locus_tag="OQS60_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCS2386977.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         9287 a         9220 c         9635 g         9143 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agcgaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct gccgctgttt
    10801 gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt accggcctcc
    10861 attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc gcagtatgac
    10921 gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc
    10981 gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac
    11041 aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga
    11101 ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc
    11161 tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt
    11221 gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc
    11281 cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat
    11341 gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc
    11401 gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc
    11461 gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg
    11521 gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg
    11581 ctggcggaga cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg
    11641 catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt
    11701 gttggcgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc
    11761 aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc
    11821 gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct
    11881 aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa
    11941 accactggta tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca
    12001 gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg
    12061 cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca
    12121 tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt
    12181 ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt
    12241 gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa
    12301 gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg
    12361 ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc
    12421 cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag
    12481 agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg
    12541 actgacgatg aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt
    12601 tcgagcacgc agtcgctggt gcagtggctg cgcgctgcaa aattatctgc tgtttttaac
    12661 cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac atagagcaaa
    12721 tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc
    12781 taaagttatt cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct
    12841 tttcttacac cccgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa
    12901 cttttgttcg tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa
    12961 aatggctttc gggtctggct gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    13021 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg
    13081 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    13141 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    13201 gctttcttga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    13261 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    13321 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    13381 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    13441 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    13501 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    13561 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    13621 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    13681 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    13741 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca
    13801 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    13861 atatttccga ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg
    13921 cggaagtccg tgagaacccg ggtatttatc ctccggcgga cgttcgtgcg aagctattca
    13981 ctctgaaagt acaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    14041 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc agccggcaat
    14101 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    14161 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    14221 ctcaccaaat cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa
    14281 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    14341 ctggcaggtt ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca
    14401 caggtgccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    14461 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    14521 gaaattgcca gtcgggtcca tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    14581 cgcaaaccgc atcagctttc cggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    14641 gccaaacgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
    14701 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    14761 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgtatcgc cattatgaat
    14821 cgcgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc aactacccgc
    14881 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
    14941 gaagatggcc tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
    15001 gcttcagtgg tcgataacgt gccggtacac gtagcgctgc gcccggaaaa aatcatgctt
    15061 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    15121 tatctcggcg atctttcggt gtatcacgtt cgactgaaaa gtgggcagat gatcagcgcc
    15181 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    15241 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
    15301 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    15361 caaatgaagc atgggcgcaa actggtcatt gcgttgctat atatctggtt gatcttgctg
    15421 tttctgctgc catttctgat tgtctttaaa ataagcctgg gagagatggc gcgcgctatt
    15481 ccaccttata ccgaactgat ggagtgggct gacgggcaac tttccatcac tcttaatctc
    15541 ggtaattttc tgcaactgac cgacgatccg ctctattttg atgcttatct ccagtcgtta
    15601 caggtggcgg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    15661 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    15721 tggacctcgt ttctgatccg cgtttatgcc tggatgggaa tattaaaaaa caacggtgtg
    15781 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tctgcatacc
    15841 aatctggccg tttatatcgg cattgtttac gcttacgtgc cgtttatggt actgccgatt
    15901 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    15961 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    16021 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    16081 ggcccggaca gcatcatgat cgggcgcgtg ttatggcaag agtttttcaa taaccgcgac
    16141 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    16201 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    16261 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    16321 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tgggccggct
    16381 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    16441 taagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    16501 cggtggtgct ggtgcgtttt ggcaggtttc gcggatcaaa tggttttgcc tttatgatca
    16561 ccgcgccgct ggtgatgcca gatggcatca cgggcttgtc gctgttgtta ttattcgtcg
    16621 cgcttgctca tgccattggc tggcctgcgg accgcggtat gctcaccatc tggctggcgc
    16681 atgtcacttt ttgtacggct tatgtggcgg tcgttatttc ttcaagattg cgggaactgg
    16741 atcgctcgat agaagaagca gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    16801 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    16861 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    16921 cgatgctggt cttttccagc gtgcggatgg gagtgaatcc ggaaatcaac gcccttgcaa
    16981 cgttaattct cggtgcggtc ggaattgtcg gatttatcgc ctggtatctg atggctcgcg
    17041 cagaaaaaca gcggatacgc gatatccagc gtgcaagaca tggctgaaga cactaaaatt
    17101 tgccaacctg gctacataat gccgcgcatg ccgcggcatt gttttcatgg aagacgaaac
    17161 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    17221 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    17281 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    17341 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    17401 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    17461 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    17521 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    17581 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    17641 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    17701 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    17761 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    17821 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    17881 aaccactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    17941 tttatcctgc ctcttttgcg cccgtttttg cggcgctaaa accgtttatc gcccgtgcgg
    18001 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    18061 aaagccagag cgatggcggc atgatgctgc gctttgtact gcgttctgaa accaaactgg
    18121 cacaactgcg taaggcgctg ccgtggttac aggaacaact accgcaactg aaagttatta
    18181 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    18241 ctgaacaaca ggcgctggca gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    18301 gtttcttcca gactaatccg gcggtcgcca gccagctgta cgctaccgcg cgcgactggg
    18361 tacggcagct gccagttaaa catatgtggg atctgttctg cggtgtgggt ggctttggtt
    18421 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcatca gaggccattg
    18481 cctgtgcaaa gcagtcagcc gctgaactgg gcttaacgcg tttgcaattt caggcgctgg
    18541 actccactca gtttgccacc gctcaggggg atgtgccgga gctggtgctg gttaacccgc
    18601 cgcgccgcgg cattggtaaa ccgctgtgtg attatctctc aacgatggca ccgcgtttta
    18661 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    18721 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtga
    18781 tgacgctgct ggtgaagcaa taaaaaagcc gcaggtgcgg cttcagattg ctgatcaggt
    18841 gcgctttgtt catgccggat gcggcgtgta cgccttatcc ggcctacaaa atcatgctaa
    18901 atcaatacat tgcagagttg atgtgggcca gataagcgca gcgcatcagg caatcttact
    18961 tttgtcatca gtctcaatta ctgcgggaac cactggtcat tgattttctg atatgtaccg
    19021 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    19081 cgtacagcga tgcccagacc ggtgccgaag tattgcggat cggtcacttt ttcagtagca
    19141 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc
    19201 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    19261 gttttcactt cggggtgctg atcctgaata tatttctggt gcgtggtgcc gttttccatc
    19321 ccaatacgtt tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg
    19381 acggctgagt tttcatagta gggcgtggta aacgatacct gcttgctacg ctcgggcgtg
    19441 atatccatac cggagattac ggagtcatat tttctgaatt tcagtgacgg gatcaggctg
    19501 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    19561 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    19621 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    19681 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    19741 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaat
    19801 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    19861 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    19921 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    19981 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    20041 agacgcatca tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg
    20101 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    20161 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    20221 gaagagagcg agcgtttaaa ggcatacggc agcaggagcg ccagcgtatc ttttttgctc
    20281 attcccaggg cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa
    20341 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    20401 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    20461 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    20521 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    20581 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct
    20641 ttcatcagtt cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    20701 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    20761 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca
    20821 gcattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    20881 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    20941 agatagccga tttcgacagc cccagcgcct gaccagactc ccactgaccc accggcaccg
    21001 ctttcaacgc gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    21061 tgacaccaca aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct
    21121 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc
    21181 caaaatagat aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg
    21241 ctgaacctgc ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg
    21301 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    21361 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    21421 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct
    21481 cagtgttgcc ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca
    21541 ctttgtcgcc caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg
    21601 tgtcaccgaa gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    21661 tgtcatacgg aacggtagtg atttccgggt gcttatccat aatgaatttc tggtgtgtcg
    21721 tcccgttctg tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    21781 gctgacccac aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt
    21841 cacgctccgg agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc
    21901 ttgggatcag gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac
    21961 acagcgcttg tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    22021 caaacggagg ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    22081 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    22141 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    22201 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    22261 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    22321 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    22381 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    22441 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    22501 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    22561 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    22621 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    22681 gcctgcaatg ttgagcgtac cggagcgtgg catctcaagc agattgagta cacgcagcag
    22741 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    22801 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    22861 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    22921 tttgacagaa taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc
    22981 aataaacata gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct
    23041 cacgaaatgc cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga
    23101 tggactgacg gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgcacatcgt
    23161 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccaa ccctggtcac
    23221 cctgtttgag atctacgcgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    23281 cactggcaac gtgggcgcta tctggcaagg tggttcgact ggagaacgga tcgctgctta
    23341 acaattcacg atgactatta tcgcggctgg catcgctaag aagggttgcc agtttgtcac
    23401 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcataga
    23461 attgttgagc cacgctgtca ggccccccct ggacacagga accggtgcgg ggaccgttat
    23521 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    23581 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    23641 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    23701 aacacctgat tgggtttgag tccattgctg gctatccact caaggttgct cgtagctgac
    23761 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    23821 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    23881 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    23941 acgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    24001 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    24061 ttgactctcg ataatattat ttatgctaca attgtggcta aagaatttaa gctaatgaat
    24121 tttagcgaag aataaattaa gttgtttcag tatctcgtta attaatatga atataaaagg
    24181 cgttgtgctt ttataattat aaagcacgag gagttcggtg gattatcgtt tttcttgggt
    24241 gatttttaat atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact
    24301 aaatcaaagt gataatttcc tggattcact gagggaattt ttcgcgatat taaattccag
    24361 tcgtaagggt gagttgtcta catgggatac catctacttg catctgatat tagcaataaa
    24421 tgctgacagt gatctcataa aaaatgatgt gttgctcgcg gaaaatatgc catcagcgaa
    24481 ttaccaattt aatacttttt ttagtaatgc tttcgagact gatgttaaaa aatatctggg
    24541 taaatcagaa gataacgaag ttgaaataaa ggcgggtaat gaaagaatct ctattggaat
    24601 acgtaatgta tcgaatggga aacttgaaag acaacagttt cttttccctc tggactatga
    24661 gaataaatta caagatcaat tagacaagca ttttacgatt gaatcgcacc cattattata
    24721 caggcatacc attggtagta aaattgcgaa tgtgattttt gaaaaattat acagccgtat
    24781 tgactttaac aaagaacaat atatatcctt tataaagggc gcgtttattc atttttatga
    24841 ttatagcagg cgatatgcaa ttagtgagaa tatcgaaaaa gacgctgtaa caaacaatat
    24901 cgcattgatg aacacttttt atgacagtga taatacgtca ggtgaggttt taaataatga
    24961 ttttacagaa gaagagccat ttgaaactgc gttagatgta gagcatacaa tagtgcttgg
    25021 atttgctgat gataattttg aaacaaaacc tgttcattat caggatttat taacacgatt
    25081 tagtgccttt caagataccg tgtttaatct gtttcctgaa atgcgctctt cgcattacca
    25141 cgatatttgt agcgtatgtg tggatatgac aaagggaacg caatgcatga ttcacttgat
    25201 ggtgaatgaa gaagttttca tgtctctgcc tgttccagtt gccacgatgg tacgtgaaga
    25261 tgcaagtaat ttagtcaatc ttaaaacact actcaatgat ggttttttca ttaaatatag
    25321 tcattttaat gacgtggcat taataaagca aaacataagt aatctctact tatcacacac
    25381 tgttgtgaat gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt taggtgatgt
    25441 taaaataaca aatagcaata taataaataa tgtattcaaa aatgtttcgt ttagatctgt
    25501 taaaattaac aacgtaaata ctcattcttt aaaatttatt aattgccatt tttttaatgt
    25561 tgatatgata agagttaatt tcagcaaatg tctttttcat gactgtagta tgcatggtgt
    25621 caagataaaa ccatggttgc cagtaaaatg gacaaaagag ttaataaatg attatttgta
    25681 tggctgttta ctatctctgt actctatctg tgcaagggat atatataata tgagcgccgg
    25741 gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaagaataa
    25801 atattgcatt aaactattaa gcgctcagga acgggcgttt atttacgaat ttgcaagaat
    25861 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
    25921 gtctgaccaa aaagcaatcc agaggtatat acctcagtca caagatgggg aagactttag
    25981 gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt gcaacataat
    26041 aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt
    26101 aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
    26161 cttcccaaca tggcatatca gttcgataat gctttagaga tcgaaggctt aacaaacaag
    26221 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca tcgttgaata
    26281 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
    26341 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
    26401 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
    26461 cgtcggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
    26521 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
    26581 gctctgttat tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
    26641 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
    26701 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
    26761 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
    26821 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
    26881 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggccgga
    26941 gtgaaatatt ttgccccgtt tgaaccgaca cagattcagg cgcttattcc actggcgaaa
    27001 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
    27061 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
    27121 attggtgcct ggccggatgc gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac
    27181 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
    27241 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
    27301 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
    27361 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
    27421 ttctcgatac cttcatctag ggtgatgatc ggctgataac ctaactcttc ctgcgcacgc
    27481 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagcggcggc
    27541 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
    27601 taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
    27661 ctgcgcagtg tgcgatgctc gccgttggtg atattgtaca cacgcccgga aggtagctta
    27721 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg tattttcata gtaggtcata
    27781 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
    27841 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
    27901 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
    27961 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
    28021 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
    28081 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
    28141 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
    28201 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac aaactctgcg
    28261 cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg
    28321 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
    28381 agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt
    28441 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
    28501 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
    28561 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
    28621 catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt cccagccccg
    28681 acgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atcactttcc
    28741 agctatccac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
    28801 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
    28861 aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg ctggcggacg
    28921 ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc cagcgggcaa
    28981 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc tctttcaacg
    29041 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
    29101 tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
    29161 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
    29221 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
    29281 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca
    29341 acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
    29401 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
    29461 ctgaacctgc gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac
    29521 gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg
    29581 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
    29641 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
    29701 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
    29761 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
    29821 gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
    29881 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
    29941 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
    30001 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
    30061 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
    30121 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
    30181 tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatcccg
    30241 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
    30301 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
    30361 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
    30421 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct tgcgggtaaa ttcccatgct
    30481 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttctag actgagtaat
    30541 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
    30601 agcgtgccgt cggcagccgc gtcgatgggt tgcggttgaa tactgcccag caccgctgcg
    30661 ccaccggctt caaacagata attatgcgcg gcctgaccga caatatactc ttcgccgcgt
    30721 tcgcagtgac tgagcagagc gaccaggtta gcctgggtgc cggtcggcag aaaaatggcg
    30781 gcttctttac cggaaagctc tgcggcgtag tcctgcagag cattaacggt agggtcgtct
    30841 ccgtaaacgt cgtccccaac cggggcggcc atcattgctt cgagcatggc gcggctcggt
    30901 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
    30961 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
    31021 ctgatgattg cgcgcaacat atacaagcta aatcctttgg cctgttcgag tttgatctgc
    31081 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccagcaccgg accgtcgatg
    31141 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
    31201 atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc
    31261 aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg
    31321 acaattttca ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta
    31381 aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc
    31441 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
    31501 ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg
    31561 aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
    31621 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc
    31681 agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc
    31741 agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
    31801 gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga
    31861 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
    31921 aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta
    31981 ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta
    32041 tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt
    32101 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac
    32161 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca
    32221 accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc
    32281 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
    32341 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
    32401 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
    32461 gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg
    32521 gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta
    32581 agactgtcac taagaccgtt cagagagtcg cctgtgactc cccagatgcg tttcacccct
    32641 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
    32701 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag
    32761 aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat
    32821 tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
    32881 atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt
    32941 gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt
    33001 tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt
    33061 ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gagtgaagaa tttctcttta
    33121 aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc
    33181 gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga
    33241 gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga
    33301 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
    33361 cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg
    33421 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
    33481 tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
    33541 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct
    33601 ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt
    33661 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
    33721 tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc
    33781 atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
    33841 tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac
    33901 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
    33961 tgacgatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttcacac
    34021 cacagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac
    34081 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
    34141 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
    34201 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
    34261 gacgcagttt ttcacggctc accagatcaa tcagcgtatc agcagcgcca agcagcgtct
    34321 ggcgaccaaa acccacggtg ataaggtgcg gaatttcgct gtacgggaag cctgccatct
    34381 gttgcgcctg ggtgataacc gcagagaaat catcaccgtc cagatgacgc acgccaggcc
    34441 agccaacaat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
    34501 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct
    34561 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
    34621 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
    34681 gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg
    34741 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
    34801 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
    34861 tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat
    34921 cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc
    34981 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
    35041 taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc accagttgca
    35101 ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgtgctttca
    35161 gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
    35221 taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga
    35281 tgatgccgta ttcacacgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
    35341 gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc
    35401 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
    35461 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
    35521 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
    35581 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
    35641 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
    35701 gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg
    35761 gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa
    35821 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    35881 ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc
    35941 gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg
    36001 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag
    36061 gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    36121 tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag
    36181 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc
    36241 tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    36301 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    36361 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa
    36421 cgcagcttgt tggcgaagcg gaatgaggta acctacaatc aggggaacca gaatgattaa
    36481 cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    36541 cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg
    36601 agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc
    36661 gatgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga
    36721 tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag
    36781 ggataagtcg agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggggcaggg
    36841 ggcggcggcg attggccgcc ccctgcgcgc tccttgcgcc agtggcaata tgtggcttag
    36901 ctcatgaaag gagcgcaaca agatgatgaa tcaacatata acaacatctt aaaaaaaggc
    36961 ctgacattac gccaggcctt ctgcgttaat taatcacgct tttccagcag ggtccggtaa
    37021 atcagaccac cgataatgcc gccgacaatt ggcaccaccc agaagaacca cagttgttct
    37081 aatgcccagc cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg
    37141 ttagtcaccg gaatactaat taagtgaatc agggttaagg ccagaccaat agcgatcggc
    37201 gcaaaacctg ccggcgcgaa tttgtcggtt gcgccgtgga tcaccaacag gaaacctgca
    37261 ctcaatacca gttcaactac cagcg
//
LOCUS       DAJVYM010000025        36326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVYM010000025 DAJVYM010000000
VERSION     DAJVYM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.025"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OQS60_001168"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OQS60_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS2386978.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OQS60_001169"
     CDS             1867..1992
                     /locus_tag="OQS60_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386979.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OQS60_001170"
     CDS             complement(1989..2060)
                     /locus_tag="OQS60_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386980.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OQS60_001171"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OQS60_001171"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS2386981.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OQS60_001172"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OQS60_001172"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS2386982.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OQS60_001173"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OQS60_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS2386983.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OQS60_001174"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OQS60_001174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS2386984.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OQS60_001175"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OQS60_001175"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS2386985.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OQS60_001176"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OQS60_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS2386986.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OQS60_001177"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OQS60_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS2386987.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OQS60_001178"
     CDS             complement(9465..9872)
                     /locus_tag="OQS60_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS2386988.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OQS60_001179"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OQS60_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS2386989.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OQS60_001180"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OQS60_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS2386990.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OQS60_001181"
     CDS             complement(11538..12197)
                     /locus_tag="OQS60_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS2386991.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OQS60_001182"
     CDS             complement(12361..12672)
                     /locus_tag="OQS60_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS2386992.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OQS60_001183"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OQS60_001183"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS2386993.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OQS60_001184"
     CDS             complement(14207..14950)
                     /locus_tag="OQS60_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2386994.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OQS60_001185"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OQS60_001185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS2386995.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OQS60_001186"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OQS60_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS2386996.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OQS60_001187"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OQS60_001187"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS2386997.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OQS60_001188"
     CDS             20005..20088
                     /locus_tag="OQS60_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2386998.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OQS60_001189"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OQS60_001189"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS2386999.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OQS60_001190"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OQS60_001190"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS2387000.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OQS60_001191"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OQS60_001191"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS2387001.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OQS60_001192"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OQS60_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS2387002.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OQS60_001193"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OQS60_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS2387003.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OQS60_001194"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQS60_001194"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OQS60_001195"
     CDS             25234..25836
                     /locus_tag="OQS60_001195"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS2387004.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OQS60_001196"
     CDS             complement(25937..25996)
                     /locus_tag="OQS60_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2387005.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OQS60_001197"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OQS60_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS2387006.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OQS60_001198"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OQS60_001198"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS2387007.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OQS60_001199"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OQS60_001199"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS2387008.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OQS60_001200"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OQS60_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS2387009.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OQS60_001201"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OQS60_001201"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS2387010.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OQS60_001202"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OQS60_001202"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS2387011.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OQS60_001203"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OQS60_001203"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS2387012.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OQS60_001204"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OQS60_001204"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS2387013.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36326)
                     /locus_tag="OQS60_001205"
     CDS             complement(35716..>36326)
                     /locus_tag="OQS60_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2387014.1"
                     /translation="IGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDR
                     QLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRF
                     QNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPAT
                     LKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8701 a         9741 c         9170 g         8714 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatca
//
LOCUS       DAJVYM010000026        35777 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVYM010000026 DAJVYM010000000
VERSION     DAJVYM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.026"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..581)
                     /locus_tag="OQS60_001206"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OQS60_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQS60_001207"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQS60_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS2387015.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQS60_001208"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQS60_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS2387016.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQS60_001209"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQS60_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS2387017.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OQS60_001210"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OQS60_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS2387018.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OQS60_001211"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OQS60_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS2387019.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OQS60_001212"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OQS60_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS2387020.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OQS60_001213"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OQS60_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS2387021.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQS60_001214"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQS60_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS2387022.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OQS60_001215"
     CDS             complement(10994..11137)
                     /locus_tag="OQS60_001215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387023.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OQS60_001216"
     CDS             11138..11416
                     /locus_tag="OQS60_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387024.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OQS60_001217"
     CDS             complement(11463..13559)
                     /locus_tag="OQS60_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS2387025.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OQS60_001218"
     CDS             complement(13559..14296)
                     /locus_tag="OQS60_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS2387026.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OQS60_001219"
     CDS             complement(14293..14931)
                     /locus_tag="OQS60_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS2387027.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OQS60_001220"
     CDS             complement(15045..15287)
                     /locus_tag="OQS60_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387028.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQS60_001221"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQS60_001221"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS2387029.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OQS60_001222"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OQS60_001222"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS2387030.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OQS60_001223"
     CDS             19524..19796
                     /locus_tag="OQS60_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS2387031.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQS60_001224"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQS60_001224"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS2387032.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OQS60_001225"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OQS60_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OQS60_001226"
     CDS             22056..22862
                     /locus_tag="OQS60_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2387033.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OQS60_001227"
     CDS             22872..23279
                     /locus_tag="OQS60_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS2387034.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OQS60_001228"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OQS60_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OQS60_001229"
     CDS             23839..24636
                     /locus_tag="OQS60_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS2387035.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OQS60_001230"
     CDS             24647..25471
                     /locus_tag="OQS60_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS2387036.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OQS60_001231"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OQS60_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OQS60_001232"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OQS60_001232"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS2387037.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OQS60_001233"
     CDS             complement(27914..29545)
                     /locus_tag="OQS60_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS2387038.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OQS60_001234"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OQS60_001234"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS2387039.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OQS60_001235"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OQS60_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS2387040.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35777
                     /gene="espL4"
                     /locus_tag="OQS60_001236"
                     /pseudo
     CDS             34789..35777
                     /gene="espL4"
                     /locus_tag="OQS60_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9230 c         9100 g         8849 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaagga
//
LOCUS       DAJVYM010000027        34981 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVYM010000027 DAJVYM010000000
VERSION     DAJVYM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.027"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..815)
                     /locus_tag="OQS60_001237"
     CDS             complement(<1..815)
                     /locus_tag="OQS60_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2387041.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OQS60_001238"
                     /pseudo
     CDS             907..993
                     /locus_tag="OQS60_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OQS60_001239"
     CDS             complement(1397..1873)
                     /locus_tag="OQS60_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS2387042.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OQS60_001240"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OQS60_001240"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS2387043.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OQS60_001241"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OQS60_001241"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS2387044.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OQS60_001242"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OQS60_001242"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS2387045.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OQS60_001243"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OQS60_001243"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS2387046.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OQS60_001244"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OQS60_001244"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS2387047.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OQS60_001245"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OQS60_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS2387048.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OQS60_001246"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OQS60_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS2387049.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OQS60_001247"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OQS60_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS2387050.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OQS60_001248"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OQS60_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS2387051.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OQS60_001249"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OQS60_001249"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS2387052.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OQS60_001250"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OQS60_001250"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS2387053.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OQS60_001251"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OQS60_001251"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS2387054.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OQS60_001252"
     CDS             13848..14552
                     /locus_tag="OQS60_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS2387055.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OQS60_001253"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OQS60_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OQS60_001254"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OQS60_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OQS60_001255"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OQS60_001255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS2387056.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OQS60_001256"
     CDS             complement(17484..18245)
                     /locus_tag="OQS60_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS2387057.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OQS60_001257"
     CDS             18378..18788
                     /locus_tag="OQS60_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS2387058.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OQS60_001258"
     CDS             complement(18750..19856)
                     /locus_tag="OQS60_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2387059.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OQS60_001259"
     CDS             complement(19867..21000)
                     /locus_tag="OQS60_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2387060.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OQS60_001260"
     CDS             complement(20993..22729)
                     /locus_tag="OQS60_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2387061.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OQS60_001261"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OQS60_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS2387062.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OQS60_001262"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OQS60_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS2387063.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OQS60_001263"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OQS60_001263"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS2387064.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OQS60_001264"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OQS60_001264"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS2387065.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OQS60_001265"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OQS60_001265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS2387066.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OQS60_001266"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OQS60_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS2387067.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OQS60_001267"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OQS60_001267"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS2387068.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OQS60_001268"
     CDS             complement(31413..31673)
                     /locus_tag="OQS60_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2387069.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OQS60_001269"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OQS60_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS2387070.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OQS60_001270"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OQS60_001270"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS2387071.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OQS60_001271"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OQS60_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8275 a         8974 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctgga ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAJVYM010000028        33482 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVYM010000028 DAJVYM010000000
VERSION     DAJVYM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.028"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(64..1521)
                     /gene="nuoN"
                     /locus_tag="OQS60_001272"
     CDS             complement(64..1521)
                     /gene="nuoN"
                     /locus_tag="OQS60_001272"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS2387072.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(1528..3057)
                     /gene="nuoM"
                     /locus_tag="OQS60_001273"
     CDS             complement(1528..3057)
                     /gene="nuoM"
                     /locus_tag="OQS60_001273"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS2387073.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3221..5062)
                     /gene="nuoL"
                     /locus_tag="OQS60_001274"
     CDS             complement(3221..5062)
                     /gene="nuoL"
                     /locus_tag="OQS60_001274"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS2387074.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5059..5361)
                     /gene="nuoK"
                     /locus_tag="OQS60_001275"
     CDS             complement(5059..5361)
                     /gene="nuoK"
                     /locus_tag="OQS60_001275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS2387075.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5358..5912)
                     /gene="nuoJ"
                     /locus_tag="OQS60_001276"
     CDS             complement(5358..5912)
                     /gene="nuoJ"
                     /locus_tag="OQS60_001276"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS2387076.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(5924..6466)
                     /gene="nuoI"
                     /locus_tag="OQS60_001277"
     CDS             complement(5924..6466)
                     /gene="nuoI"
                     /locus_tag="OQS60_001277"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS2387077.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6481..7458)
                     /gene="nuoH"
                     /locus_tag="OQS60_001278"
     CDS             complement(6481..7458)
                     /gene="nuoH"
                     /locus_tag="OQS60_001278"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS2387078.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7455..10181)
                     /gene="nuoG"
                     /locus_tag="OQS60_001279"
     CDS             complement(7455..10181)
                     /gene="nuoG"
                     /locus_tag="OQS60_001279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS2387079.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10234..11571)
                     /gene="nuoF"
                     /locus_tag="OQS60_001280"
     CDS             complement(10234..11571)
                     /gene="nuoF"
                     /locus_tag="OQS60_001280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS2387080.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(11568..12068)
                     /gene="nuoE"
                     /locus_tag="OQS60_001281"
     CDS             complement(11568..12068)
                     /gene="nuoE"
                     /locus_tag="OQS60_001281"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS2387081.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12071..13873)
                     /gene="nuoC"
                     /locus_tag="OQS60_001282"
     CDS             complement(12071..13873)
                     /gene="nuoC"
                     /locus_tag="OQS60_001282"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS2387082.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(13967..14629)
                     /gene="nuoB"
                     /locus_tag="OQS60_001283"
     CDS             complement(13967..14629)
                     /gene="nuoB"
                     /locus_tag="OQS60_001283"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS2387083.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(14645..15088)
                     /gene="nuoA"
                     /locus_tag="OQS60_001284"
     CDS             complement(14645..15088)
                     /gene="nuoA"
                     /locus_tag="OQS60_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS2387084.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(15717..16655)
                     /gene="lrhA"
                     /locus_tag="OQS60_001285"
     CDS             complement(15717..16655)
                     /gene="lrhA"
                     /locus_tag="OQS60_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS2387085.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            17570..18787
                     /gene="alaA"
                     /locus_tag="OQS60_001286"
     CDS             17570..18787
                     /gene="alaA"
                     /locus_tag="OQS60_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS2387086.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            18871..19470
                     /gene="yfbR"
                     /locus_tag="OQS60_001287"
     CDS             18871..19470
                     /gene="yfbR"
                     /locus_tag="OQS60_001287"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS2387087.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(19529..21361)
                     /locus_tag="OQS60_001288"
     CDS             complement(19529..21361)
                     /locus_tag="OQS60_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS2387088.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21448..22096)
                     /gene="hxpA"
                     /locus_tag="OQS60_001289"
                     /pseudo
     CDS             complement(21448..22096)
                     /gene="hxpA"
                     /locus_tag="OQS60_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22107..22601)
                     /locus_tag="OQS60_001290"
     CDS             complement(22107..22601)
                     /locus_tag="OQS60_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS2387089.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(22684..23139)
                     /locus_tag="OQS60_001291"
     CDS             complement(22684..23139)
                     /locus_tag="OQS60_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS2387090.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23477..24679
                     /gene="ackA"
                     /locus_tag="OQS60_001292"
     CDS             23477..24679
                     /gene="ackA"
                     /locus_tag="OQS60_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS2387091.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            24754..26898
                     /gene="pta"
                     /locus_tag="OQS60_001293"
     CDS             24754..26898
                     /gene="pta"
                     /locus_tag="OQS60_001293"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS2387092.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27088..28608
                     /gene="yfcC"
                     /locus_tag="OQS60_001294"
     CDS             27088..28608
                     /gene="yfcC"
                     /locus_tag="OQS60_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS2387093.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(28641..29183)
                     /gene="yfcD"
                     /locus_tag="OQS60_001295"
     CDS             complement(28641..29183)
                     /gene="yfcD"
                     /locus_tag="OQS60_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS2387094.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29241..29792)
                     /gene="yfcE"
                     /locus_tag="OQS60_001296"
     CDS             complement(29241..29792)
                     /gene="yfcE"
                     /locus_tag="OQS60_001296"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS2387095.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(29848..30492)
                     /gene="yfcF"
                     /locus_tag="OQS60_001297"
     CDS             complement(29848..30492)
                     /gene="yfcF"
                     /locus_tag="OQS60_001297"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS2387096.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            30628..31275
                     /gene="yfcG"
                     /locus_tag="OQS60_001298"
     CDS             30628..31275
                     /gene="yfcG"
                     /locus_tag="OQS60_001298"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS2387097.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31332..31694
                     /gene="folX"
                     /locus_tag="OQS60_001299"
     CDS             31332..31694
                     /gene="folX"
                     /locus_tag="OQS60_001299"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS2387098.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            31715..32608
                     /locus_tag="OQS60_001300"
     CDS             31715..32608
                     /locus_tag="OQS60_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS2387099.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(32656..>33482)
                     /gene="rpnB"
                     /locus_tag="OQS60_001301"
     CDS             complement(32656..>33482)
                     /gene="rpnB"
                     /locus_tag="OQS60_001301"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS2387100.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8335 a         9181 c         8426 g         7540 t
ORIGIN      
        1 tgtcagggca ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt acaaactgat
       61 agcttacatc agcggcattg ctaaccgcac aatgctaatc agcggttgtg gccatacacc
      121 cagcaccagt accaacagtg cagagatcag caccacgata ccgcctgcgc tgtactgcca
      181 gtttgacggt gcatcgcgac ccggttgttc cggggcgtgc aggtacaggc tcaccgccac
      241 gcgcaggtag tagtaaaggc cgcttgccga accgacaacc acggcgccca ccagccacca
      301 caagtgtgcc tggacaccga ccgccagcac gtagaactta ccaataaagc ccagcgtcat
      361 cggaataccg gccagagaca gcatcatcac cgtcatcacc gccgcgagga tcgggcgatg
      421 ccagaacagt ccgcggtaag agtacaggga atcagcatcc gggccacgat acgggctgga
      481 catcaggctg accacgccga acgcgccgag gctgctaaac agataaccgg ccaggtaaac
      541 ccctaccgct tccatcgaca tctcgccagt ttgcagcgca atcagcgcca ccagcagata
      601 gccgaggtga gagatagatg agtaaccgag cagacgtttg atattggtct ggctcagcgc
      661 catcaggtta ccgaagatga tggaggcaaa ggcgataatc gccagcacca cgcgaatcgc
      721 ttcgctgtca cccatcggtg cgtagaggaa cagacgcatc accacaccga agatagcgat
      781 tttgctcgcc gtcgccaaga aagtggaaac cggcgcaggc gcgccctggt atacgtctgg
      841 cgtccacagg tggaacggca ccagagagag tttgaagccg aggccaacaa tcatcaggcc
      901 gaaacctgcc agcaacagcg gctcgttgag cataccgtcg ccaaggtttt tacccaacgc
      961 gacaaacgac aggtcgccag actgcgcata caccagcgcc ataccaaaca gcaggaaaga
     1021 agacgctgcg gcagaaagga tggtgtattt gatactggct tccagtgaac gtttctggcg
     1081 gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc agttcgatac cgaggaacag
     1141 agacgccaga tggttggcat tcgccagcag gatcccgccc agcgcggcaa ttaacaccaa
     1201 caggtagaac tcgtccttgt tgtcgttata gccttcaagc cacgggtagg cgaaagtaca
     1261 ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac catcaacgcg
     1321 catcagcggc gtaacgtcca tagcgcctgc ctggccgaca aaccagagcg aaaccagcgc
     1381 cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat
     1441 ggagagcatc acaaccacca ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag
     1501 gttttgtgga gttattgtca tggcgattta cggccttgta gtagtaacgg aattaacaaa
     1561 ccactgctgg atattgccaa tcgcggagtg cgaggtatcc agaatcggct gcggatagaa
     1621 gccctgcagt accagcagca ccaccagcaa caggatcata aacagctcac gcagcgacat
     1681 ccctggcagt tcctggctgg caatctcgct tttcgcttta ccgaagtaag cgcgatgcaa
     1741 catcgccagc gaataaacag atgcaaagac cagcccaaag gtagagataa cggtaatcac
     1801 cgggacaacc tggaagctgc cgaacagaat cataaattcg ccgacgaagt taccggtgcc
     1861 aggcatccca agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc atttcatctt
     1921 gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc
     1981 acaaagaata aacagacccg ccgccgacaa gccgtgcgca atcatctgga ttaccgcgcc
     2041 ctggtaggcc aactggctgc cggtgtagat agcaatcagc acgaagccca tgtgggaaac
     2101 cgaggtgtag gcgatcagac gtttgatatc ggtctgggcg aaggccatcc acgcaccgta
     2161 gaagatgccg ataacaccca gccacatagc aattggcgcg aactctgccg acgcgttcgg
     2221 gaacagcggc agggagaaac gcagcaaacc gtaagcggca gttttcagca agatccccgc
     2281 gaggtcaacg gaaccggcgg tcggagcctg ggagtgcgca tccggcagcc agccatgcag
     2341 cggaaccacc ggcattttga ctgcgaaggc gatgaagaag cccagcatta acaggtattc
     2401 cgcaccactg gacattggcg tattcagcag ctcttcatag ttgaaggtcc agacgccggt
     2461 cgcattgtag tgaacaaaaa ccagcgccag gatggcaatc aacatcacca gaccactcgc
     2521 ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc gttttaccgt cagaggcttt
     2581 atgcccccac agtgcgatca ggaagtacat cggcaccagc atcatttccc agaagaagaa
     2641 gaacaggaac atgtcgatgg caaggaacac gccgataacg ccgcccagga tccacatcag
     2701 gttgaggtgg aagaagccct gatatttttc gatctctttc cacgaacata gtaccgccag
     2761 cacaccgagc agaccggtca gcacgaccat cagcagcgac agcccgtcaa tggcgagatg
     2821 aatagagata cccaaacgcg ggatccacgg catgtcgaat tcagactgcc actgcggaat
     2881 tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg acagcgccag
     2941 cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc ttgacgccaa agcgttcggt
     3001 ctgccagcac aggaagccgc caataaaggg aattaatatt agccagggta gtaacatggc
     3061 gatctttatt ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt ctcacgacag
     3121 aaaattctct tcggactggg ggtagtgtcg gatgcaccgc gaaggcggct tatccgacct
     3181 acgttcttat atgcccaggg gcaatcccac aatccttaac tcaacgcagt accatcaaca
     3241 gtgccagcac cacgaccgca ccgatgctca tggatgccac ataccagcgc agatagccgt
     3301 tctcacttaa cagcagacct ttacctgcaa agcgggaaag gacagccgga atgttcatca
     3361 ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc caggaacggc ctgacgaaca
     3421 ctttgtcgta cagccagtca aagccccagg cgttgtacca ccaggtactc agcaggcggc
     3481 ccggcgcact gttggcgatg gaggtcacca gagtacgttt acccagccac agccaagctg
     3541 ccagcagaat gccgacaatc gcgaccacgc cagaggtaat ttccagggtc aacatgctgc
     3601 cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca atcagtgcgc
     3661 caacgaaggt ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt acccctttca
     3721 cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat gaaaatcata cggaaggtgt
     3781 agagcgaggt cataaacgca ccgaccagac ctgccaccat cagattgata tgaccattcg
     3841 ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc agcggcagtg
     3901 ccgacagtgc tgcgccgccc accaggaagc agagataaac cagcggaata gatttacgca
     3961 gaccgcccat cttgaagatg ttctgttcgt gatggcaggc cagaatgacg gaaccggatg
     4021 ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa gtggaaaatc gccgcatccc
     4081 atgcctgcac gccaagcgcg aggaacatgt agccaatctg gctcatggta gagtaagcga
     4141 gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg
     4201 ccccgacaat gcccaccaga tgcagaattt ccggcgtcat caggaacagg ccgtgggtac
     4261 gggcgatcag gtagacaccc gcggttacca tggttgcggc gtggatcagc gcggagacag
     4321 gcgtcgggcc cgccatcgcg tcggcaagcc atgtctgcaa cggcaactgc gcagatttac
     4381 cgaccgcacc gcccagcagc atcagcgtcg cccacatcag catgttattg ccgtcagcaa
     4441 agtgcgctgg tgccagttcc accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa
     4501 gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg ggtcacgacg aacgctttca
     4561 ttgccgctgc gccattcttc ggatcggtgt aatagaaacc gatcagcaga taggagcaca
     4621 ggcccacgcc ttcccagccg aggtacatca gcagcaggtt gtcggcaagc accagaacta
     4681 ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg agagtagccc tcttcaccgc
     4741 gcatgtacca ggaggcgtac atgtgaataa ggaaacccac accagtgacc accgagagca
     4801 tggtcagcga caggccgtcc agcaccaggt taaaaccgat gttaaagtcg cctaccgaca
     4861 tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt agcgaagaaa tcaacgccga
     4921 taaaggcggt taccagcgcc gccaggccca cagagccaac gccgacgatc gccgaaacgt
     4981 tttcagacca gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa
     5041 tggttaaggc aagcatgttc atccgcgcat ctcacttact gaatcgatgt tcaggttctg
     5101 gcgacgacgg tgaagttgca gcagcagcgc aaggccgata ctcgcttctg ccgccgcgag
     5161 gctgatggcg agaatgtaca tcacctgacc gtcggtctga ccccagtagc ttccggcgac
     5221 aacaaaggcc agcgcggagg cgttaatcat gatttccaga ccaatcagca taaacagcag
     5281 attgcgacgg ataaccagac cggttaagcc aagaatgaat aagattgccg cgaggatcag
     5341 tccatgttgt aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta
     5401 cgattgctca gcacttcacc cgcacgctct tcacgaccga cgtggaaggc cacaaccaga
     5461 cctgcgagca gcagcataga agccagttcc accgccagta cgtaaggccc gaacagcgta
     5521 atacccactg ctttagcact gattggcgta ccgtcgatac cctgatcgtt aacaccgagg
     5581 atggcgtaaa caatcaccac cagcatgatg gccgacaaaa ttgccggacc aatccacacc
     5641 tgcggtttca gccactggcg ttcctgttcg atttctgaac cgcccaggtt gagcatcatc
     5701 accacgaaca cgaacagcac cataatggca cccgcgtaga cgataatttc cagcgcaccg
     5761 gcgaagtaag cgcccagtga gaagaacacc ccggaaatcg ccagcagcga aataatcagg
     5821 tacagcagtg cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc aagtatggct
     5881 atcaggccac agatataaaa agcgaactcc attgcccctc tccttacggt aacaggctct
     5941 tgacgtcgat aggcttggct tcgttctctg cttcgccctt atctttgccg tcgattgcca
     6001 tacctgccat ccggtagaag ttatattccg ggtatttgcc cggaccggag atcagcagat
     6061 cctctttttc gtaaaccaga tcctggcgct tgtattcccc catttcgaaa tccggggtta
     6121 actgaatcgc cgtggtcgga caggcttctt cgcacagacc acagaaaatg cagcgtgaga
     6181 agttgatgcg gaaaaattcc ggataccagc gaccgtcttt ggtttctgct ttttgcagcg
     6241 agatacagcc gaccgggcag gctaccgcgc agaggttaca ggctacgcaa cgctcttcgc
     6301 cgtccgggtc gcgggtcaga acgatacgac cacgataacg gggcggcaga tagatcggct
     6361 cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc atccagatac
     6421 tacgaacctg ggtgccgaaa cctactaaca attcttttaa ggtcatggtc ttattgcccc
     6481 ttattgcgcc tgccagagaa tgacagccgc cgttaccagc aagttgatca gcgtcagcgg
     6541 caggcagatt ttccagccga aggacattac ctggtcataa cgcggacgcg gtaacgacgc
     6601 acgaatcaaa atgaacatca tcataaagaa cgcggttttc agcgcgaacc agatgaatgg
     6661 cggtaacaac gggccttgcc agccaccgaa gaacagcgtc accatcaatg cagagatggt
     6721 cacaatcccg atgtactcac ccacgaagaa cagaccgaac ttcataccgg aatattcaat
     6781 gtggtaacca tccgccagtt cctgctcggc ttccggctgg tcaaacgggt gacggtgaca
     6841 taccgccacg cccgcgatgg caaaggtaat aaaaccaaag aattgcggga taacgttcca
     6901 cacatgcgcc tggctgttga cgatgtcggt catgttgaat gaaccggcct gcgccaccac
     6961 gcccatcagg gaaagcccga ggaacacttc gtagctcagg gtctgcgcag aagcacgcat
     7021 cgcacccagc aacgagtatt tgttgttact tgaccagccc gcaaacagca ccgcgtaaac
     7081 cgccagacct gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca
     7141 acccggactg actggcacaa tcgcaaaggc cagcagcagc gaggtaaagg caatcatcgg
     7201 tgccagggta aagatgacgc gatccgagaa tttcgggatc cagtcttctt taaagaacat
     7261 tttgatcatg tccgcaacca gctggagcga accgccccag ccaacacggt taggtccgta
     7321 acggttctgg aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg ccccgcaggt
     7381 gacaaccacc agcaggatca ccaccgcttt gaggatggtc agcaggatct caatcagttc
     7441 cggtgatatc caactcattg ttgtgcctcc ttgagatcct caagatgcgc gccagccagc
     7501 accggagcaa taccggacat acccatcggc aagcccacct gccctgccgt cagcccttca
     7561 gcgatttcaa ccggcagcgt gaccgtattg ccatcgtaac taaaggagac gtgtgtacct
     7621 gcgttcacac ctaacttcgc ggcatccgct gggttgagtt tgatgtacgg ctgcggcata
     7681 cggctctgga agaccggagc acgctgtgac aattcatcgc tgccaaacag gtggtaatac
     7741 ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa
     7801 tccagaccat tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg
     7861 ccgcccactt cgtcctggaa tttgttccac gcctgcgggg agttccagcc cggcgcccag
     7921 gcaaacggca cttgcgaacg gtgcgcagtc ggctggttgt taccttccat cgagaaggtg
     7981 aacatggtgt caatatcctg cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg
     8041 gcggtacgac cgctgtaacg gtgcggttca cgggccagtt tctgcccacg aatacggaat
     8101 gtcgcatccg gcgcagcatc tttgatacct gccagttccg ggattttcgc cacaacagcg
     8161 tcaatcacat ggtcgagctg cgtccagtcc acttcacggc tcagcagggt gctgtgcagc
     8221 gagtgtaacc agcgccagct ttccagcatg acagttttgc tgtcgtaata agcaggatcg
     8281 taaacctgga agaaacgttg ggcgcggcct tcgttgttga tcaccgtacc gtcgctttca
     8341 gcaaaactgg cagcagaaag taccagatgg gcgttttcca taatcgctgt gcgttgatga
     8401 tcaaccacca tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt agcggaagcg
     8461 tgacgatgca gatcgttttc cagcaccacc accgcgtcgg cgcgtccggt ttccagttcg
     8521 gtcaacgctt cttcaagcga accgccaccc ataatgccca gccccatgct gttgacggaa
     8581 cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt tcagggcttt cgcgacgtta
     8641 gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc cggagataat caacggtttc
     8701 ttcgcacctg ccagtgcctg cacgatgacg tcgattttgc tttgcagctc aggttcgata
     8761 ccgtcaaccg ctggcgcaga gttatccagc gcatgggcga tggcaaaacc taaacgcgcc
     8821 tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat catccagacg ggtgtcatca
     8881 acgttggtaa caaacagcgg atgcttcgca cgttgaccga tgttgaggat tgccgcaatc
     8941 tgccagtcag ccactttctg tgctgccgcc atttcgcgcg ctttaccttt cacagcctga
     9001 cgcactgcca gcgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag caccagtacc
     9061 gcatcgtaag attcgatttc gcgcagagcc ggagtataaa tgccgccttc acgcagcact
     9121 ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga taccggtgta gaagttttct
     9181 tcgcccacca gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg agaaccaata
     9241 ccgatcactt tcttcgactg acgcagaata tctgccgcgc cctgcattgc ctgttcggcg
     9301 ttgagggtaa tgaaatcatc gccacgacgc tgtactggct gacgcggacg atccttcagg
     9361 ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt taccgtaccg
     9421 ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt caccggggct gatgttacag
     9481 ccgatggaac attgctggca gatgctcggc gcaaactgca tatcccattt acggttgtaa
     9541 cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac caggttacca
     9601 gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgca
     9661 ccgtaaacgc ccagatctgt accgtcagcg taatctttgt agtaacgcac acagcggtag
     9721 caggcgatgc agcggttcat ttcgtgagag atgaatggcc ccaaatcctg attacggtgg
     9781 gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg tcatcacagt catatcctga
     9841 agatggcagt taccgccttc ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac
     9901 cactcgacca cactttcacg gaactgtttc gcttcttcgt cgtcaatgga aataaaggtg
     9961 ccatcggaag ccggtgtcat acaggacatc accaggcgac cacgcgtgtc ttccgcgttt
    10021 tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag
    10081 caaaagtaag gaatatcaag gcccagagac agacaagctt ccagcaggtt gtccgctccg
    10141 ttgacctcgt attctttgcc gtctacatga attgtagcca ttagcatgct tccagttttc
    10201 tcggtcaggg accgagcgtt aatcgaaatt cggttaccag cgctctttca gcaggttcgg
    10261 ctgaatccca ttaatcaaat gggtattgct gaacggctgt ttgattcccg cctcaaattc
    10321 ttcgcggaaa tatttgatgg cgctctgtaa cggctccact gcaccaggtg cgtgggcaca
    10381 gaaagtttta cccgggccta agaatcgaca cagttgctca agtgtttcga tatcgcccgg
    10441 ctggccttcg ccacgctcca gcgcacgcag aattttcacg ctccacggca gaccgtcgcg
    10501 gcacggcgta caccagccgc aggactcacg ggcgaaaaac tcttccaggt tacgcaccag
    10561 cgacaccatg ttgatctcat ggtcaaccgc catcgccagc gccgtaccca gacggctgcc
    10621 cgctttaccg atactttcga attccatcgg cagatcaagg tgcgcttcgg tcaggaagtc
    10681 agtccccgcg ccgcctggct gccaagcttt aaatttcaga ccatcacgca taccaccggc
    10741 gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc agttcccaca gtcccggatt
    10801 tttcacccga ccggagaagc ccatcagctt ggtgccagca tctttacttt tcgagatgtt
    10861 ctgataccac tccacgccgt tagcgaggat cgccggaacg ttacacaggg tttcgacgtt
    10921 gttgacacag gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg gtttcgagcg
    10981 tgggttagca cgacgccctt ccagggagtt gattaacgct gtttcttccc cgcagatgta
    11041 gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa cctgttccca taatgttttt
    11101 gccaagcaag cccgcttcag tggcttcggc aatggcgcgg cgcagattta ctgccgcttc
    11161 gatatattcg ccacgcagga agatgtagcc acggtaagct ttcagcgcaa acgcggagat
    11221 gagcatacct tccaccagca ggtgcggcag ttgctccatc aacaggcggt ctttataggt
    11281 gcccggctcc atttcatcgg cgttacacag caggtaacgg atgttcatgg attcgtcttt
    11341 cggcatcagg ctccacttca ggccagtcga aaagcccgct ccgccgcgcc ctttcagacc
    11401 agcgtctttt acctgattaa cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc
    11461 cccttcgtaa ccgtttttgc tgcggtattc gtccagccac actggctgtt tgtcatcgcg
    11521 cagacgccag gtcagcggat gcgtttcggg agtacggata atgtttttca tttataccgc
    11581 tccagcagtt cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc atcgatcatc
    11641 atgtttggcc ctttatcaca gttccccagg cagcaagttg gcagcagcgt aaagcggcca
    11701 tcaaatgtcg tttgccctgg tttgatgttc agctttttct cgagcgccgc ctgaataccc
    11761 tgatacccgt tgatatgaca gaccacgctg tcgcaataac ggatcacatg gcgaccaacc
    11821 ggctggcgga agatctgact gtagaacgtt gccacacctt cgacgtcgct tgccggaata
    11881 cccagcacat cggcgatcgc gtggatcgca ccatccggca cccagccacg ctgcttctga
    11941 acgattttca gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc
    12001 tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc
    12061 tcgtgcataa ttagcggtcc acatctgaca taacaaaatc gatactgccc agataaacaa
    12121 tcaggtcaga caccaggctg ccgcggatcg ccgccggaat ttgctgcaaa tgcgcataac
    12181 tcggcgtgcg gatacgagtg cgataactca tggtgctacc gtcgctggtc aggtagtaac
    12241 tgttgatccc cttggtcgcc tcaatcatct ggaaagattc attggcaggc atcaccggcc
    12301 cccacgacac ttgcaggaag tgggtgatca gggtttcgat atgttgcagc gtgcgctctt
    12361 tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg gccttccggc atgttgttga
    12421 ggcactgctc aagaatgcgc agactctggc gcagctcttc cactttcagc attacgcggg
    12481 tgtagcagtc agaaacacca cctccaaccg ggatttcaaa gtcgaagttt tcatagccag
    12541 aataaggacg cgccttacgc acgtcgaagt cgatcccggt agcacgcagg cccgcgccag
    12601 tggtgcccca ctccagcgcc tctttcgcgc cataggcggc aacgccctgg gaacgacctt
    12661 tcagaatggt attttgcagc gccgctttct cgtaagacgc cagacgtttc ggcatccagt
    12721 cgaggaactc acgcagcagg cgatcccagc cgcgcggcag gtcgtgcgct acgccgccaa
    12781 tacggaacca cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga tcgtaaattt
    12841 tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc tgaataaagg
    12901 tcgagatgta cagcaggtgg ctgttgatac ggaacagttc ggagagcata acgcgaatga
    12961 cgttaacgcg atccggcacg gtgatcccgg ccagtttctc taccgccagc acgtaaggca
    13021 tttcgttaac gcagccgccg aggtattcaa tacggtcggt atacggaatg tagctgtgcc
    13081 aggactggcg ctcgcccatt ttctccgcac cacggtggtg gtagccgatg tctggtacgc
    13141 agtcgacaat ctcttcgccg tcgagttgta aaacgatacg gaaagccccg tgcgccgacg
    13201 ggtggttcgg accgaggttg aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc
    13261 cccactcttc cggtttgaag gtcagggctt ccatctccag atcctgtttg gctttggtca
    13321 gctcaaacgg cgagaattcg gtagcgcgcg ccgggtaatc tttacgcagc gggtgacctt
    13381 tccaggtttg cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa
    13441 acagatccca ggtttcacgc tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg
    13501 gtacgtgcag gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga
    13561 tagaaatcag atggtagaaa acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc
    13621 gcagacgttc gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct
    13681 ttaagaaatc gccaacttcc agtaattgtt cacgcttgat ccacacaacg ggaaccccgg
    13741 tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt tcgccaatca
    13801 ccggatcatc aagatgatca cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca
    13861 tattgttcac cattgcaaat ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata
    13921 gatttaggaa taagcgaaga aaaatccctt tgccgacagg cgcaaattaa atctcgtcag
    13981 gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca
    14041 tattggcgcg ataaacgccc tgatcgccaa ccacccagga gagcggacga cgttctttgc
    14101 cgatagattc ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc ggcgggcagc
    14161 ccgggatata cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg gaataaatat
    14221 cgtacatacc accagagttg gcacaggcac ccattgagat aacccatttt ggttccagca
    14281 tctggtcata cagacgctga ataaccggtg ccattttggt aaagcaggtt cctgcaacca
    14341 ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa cgcgccacgt
    14401 catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga ccgaagttat
    14461 acggccaaat tgagttttta cgaccccagt taaccatgtc attgagcttg cccataaaca
    14521 cgtttttgtt aacttcttgc tccagagggt cggttacgat ctcctgcttt tgcagggggt
    14581 aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt ataatccatc ttaatgcctc
    14641 gcggttagcg ttgacgatta gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg
    14701 aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga cctgccagta
    14761 acacaaaaat aaaaattgca gcttccacaa agcctaccca gccgctttcg cggatagagg
    14821 ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat aacgaagaac atggccacca
    14881 gataaaactt ggcggacagg cgtaagcggg cggagccgac cgagtcgata ccggattcaa
    14941 acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca
    15001 tcaggcaaca caggccaatg gcaacgataa gaaagatagc gaatgcccag tgatgagcga
    15061 tgacttcagt ggatgttgac atactcattg cttactcatc aaaagtagcg ccagattcac
    15121 tgctcttcac ggcagatgga cgccacatcg attcatgggg aggaataaaa aaaaccttac
    15181 aatcactgta gaaattcttt tatacagcta attgatgtgg tcttttactc ctttctataa
    15241 ccttttgtca actttaacaa aagtttcttc acattagttt acataatatc aacaccatta
    15301 gcatttaatg ccctttcacg ccagatcctt gacgactcca ggataattag atgttgttga
    15361 atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta ttttgacagg aatttgtgtc
    15421 gattcctccc cccaaaagag agtattttct tgatctgtga cacgcttttg tcattccata
    15481 acaaaaacgc agcaacaaat ttacgcattt ttaacatcat tgtagcaggt gatttttttc
    15541 aggcgattat ttgtgcgttc gggacgtgaa tctctggtga ttgaaaaatg aacagttttg
    15601 tacgttctgc actatgcgag gaaggatttt tactaaaaaa aagccgctgg ggtttaaaac
    15661 acccccagcg gctcgttttt acactattgt ctcaggaatt atctatcgtc cgtcgattac
    15721 tcgatatccc tttcaatcaa caatgaatca tccccttccg gagcagacat tgggctgtat
    15781 tgccacggat tatggtagct ctccatcgcc tgatagatca cctgggccag ctcattatta
    15841 ctggacgggt catagcacag caaatattcg gtatcaggta gcggcggtaa gccatctact
    15901 ccactcagca cacgcaggta cgggctcatc atttcaatcg gccttgccgt cacgccaaga
    15961 ccggctttca ctgccgcacg aacggccgga agcgtcgagg cgacataagc cagtcgccat
    16021 ggaatatctg ctttattaag cgtcgccagc accatatcgc gaaacggact tggatcatcc
    16081 agcaatacaa gagggatcgg ctcgcctttt tgcaatacgt attccgctgc gcagtaccag
    16141 tgtgttggcg aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc
    16201 atcaaatcca cttcctggga ttccagcatt tctgccatat aggcattacg tttaacgcgg
    16261 acatccagcg ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc
    16321 gtatcggcag attcatctga agcaccgata gttaacacgc cctgaagatt actgaacatt
    16381 aatgatgagc aggcctcatc attaaaacgc agtattttcc tggcgtaacc aagaagttga
    16441 atgccatgtt cagttaacag tttgttgcga ccgtgacgag cgaacagttc tttcccaacg
    16501 agttgttcca gacgctgcat ttgctgactt acggcggact gagtacgaca cacagcggca
    16561 gctgcggcag caaaagtgtt cagatcggca acagcaacaa atgttctcag cagatcgagg
    16621 tcgaggttaa ttatcggacg atttgcactt atcatatatt atcacttact ggcggctcat
    16681 actgagctgg ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc
    16741 tggcagtttc atccggaacc cggacgaaag taaaaatgca tatgagttgc actaaaaaag
    16801 cgactcacat tgttccgtta taatgcctga agtagatcac agaatatatc ttcagggatc
    16861 gcatatctat taagttactc actcttttct atttatgaca tgcgcgtgtt tgtataaatg
    16921 taaatgtgag tccttgttcc actctcgtgc agcatcgctg gtcatacgcg aacacgtaca
    16981 aacagcaatg gtgaggcggc atcaagagca gggatccgtc atttatgcga gcattttacc
    17041 ccaaaaacct tttatttata agggtcattg cgaattatct gatgcaaagt tatgttaggc
    17101 aaagtaatct tctattatta ataagcacat caaaaccttt ttgaatatta aataataatt
    17161 aattagcatc atcctcattc attaattccg cttaacaata gtttcacacc ttgcccccat
    17221 tgcgacacct cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc
    17281 gcgtttcatt agtgaattct taaatgaggc attttcacac aattatctta cagataaaaa
    17341 aaccagactt gcaattaaga atcagaacaa ttcactatat aacattgcat gcaaagcata
    17401 tacgcctcat tattttgtca ttattaagtt attaacagca caatcgagcc ttcccctctg
    17461 gcaaaatctt attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc
    17521 tgacttccac ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat
    17581 tgaaaaatcc agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga
    17641 agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc
    17701 cccattcggt tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac
    17761 cgctcaaggg tattgcgatt ccaaagggct ttactccgcg cgtaaagcca tcatgcagca
    17821 ctaccaggct cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt
    17881 atcggagctt atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt
    17941 tcctgcacca gattacccac tctggaccgc ggcggtttcg ctttccagcg gtaaagcggt
    18001 gcattatctt tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa
    18061 aattacgcct cgtacgcgtg ggatcgttat tatcaaccca aataacccaa ccggcgcggt
    18121 atattccaaa gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat
    18181 cttcgccgat gaaatttatg acaaaattct ctacgacgat gctgagcatc actcaattgc
    18241 gccactggca cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt
    18301 tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta
    18361 catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca
    18421 cgccattcag accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg
    18481 tcgtctttat gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc
    18541 ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa
    18601 cattcacgac gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt
    18661 gcaagggacg gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg
    18721 tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca
    18781 gctgtaatct taatttcact gccggagatt gcatccggca gcgttatccc gccacaatga
    18841 cctgatgatg tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc
    18901 tcccgcctga aactcattaa ccgctggccg ctaatgcgca acgtgcggac ggaaaatgtg
    18961 tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcga
    19021 aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc
    19081 tcagaagtgc tcaccggcga tctccctact ccggtgaaat acttcaattc gcaaatcgct
    19141 caggagtaca aggctattga aaaaatcgct cagcaaaaac tggtcaatat ggttccggaa
    19201 gagctgcggg atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa
    19261 tcgctggtga aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg
    19321 gccggaaata atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt
    19381 cgcagccagg agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc
    19441 gatgagatta gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcaggcaat
    19501 aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca
    19561 cacaaaccgc catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc
    19621 tgtaatttcc cggacctaaa accagtgtgt taacaggtga agaaaccggt gtcataaagg
    19681 cggcggatgc tgccatcgcc acgaccatcg cgaatggata aggcgacacc cccatcgttt
    19741 tggcagcagc cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaata
    19801 gcccaataac cgccgacaag acaaacaaac accccagcat catatgtggc ccgtaaccac
    19861 cgccaatgtc catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca
    19921 cagcaaacgg catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg
    19981 cgctttcagc atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa
    20041 caggattagg aatttcatct gtcagcatta acgccaccat tagcaccaga cagaaaatgg
    20101 catggggtgc ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg
    20161 ctacgaagtc gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca
    20221 ggatgatatc gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat
    20281 tgcgctttag ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg
    20341 atttaccaat cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg
    20401 cctggtcaga aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa
    20461 attgccggag atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa
    20521 attcagaaac cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac
    20581 cgatgacgtt agcgccataa cgctcacgta atttgagatc atccagccgt tgaccaatca
    20641 ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga
    20701 taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt
    20761 tcagcatgaa acgcatcact aacatataca aaatacccag caccagcacg accaggccaa
    20821 taggtgttac gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga
    20881 ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc gcttatcagc ccggcaaaac
    20941 tcaacggcat catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca
    21001 cgggaataaa gatagcgaca acgccggttg aactcataaa cgcgccaagc cccgcgacgg
    21061 tcagcatcaa caaaaccaac attttgattt cactgttgcc cgcaactttg accagccatg
    21121 ttcccattac ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg
    21181 caatcaggac aacgttagga tcagaaaagc cggaaaatac ttcggggact gtcagcgttc
    21241 cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg
    21301 tcgcaaacaa gacaatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca
    21361 caacttatcc ttgttaattg agggagatgt cttgattctg ccataaaaaa gccccgacga
    21421 gacggggcta aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt
    21481 ggtcacagta atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt
    21541 atctgccgga gcgttaaccg caatgacatg acaacccgcc gccaggccag aaagcacgcc
    21601 agcggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag ctgcgcgcct
    21661 aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt tacaaacacc
    21721 tctggtgcgg gaagcccagc tattttatgg cgcgctcgcg ctaccggcat ggagccagaa
    21781 gtcacaatgg cccacggaat acctgcttta ttcaaatgac tgagtaaggc gattgccccc
    21841 ggaagcgggt aataccttcg gtttccgtgg cctcgatgtg ctccagacgc gtaaactcgg
    21901 cggcaatatc agcctcggat ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt
    21961 taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
    22021 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
    22081 aacctttgca ccgcacgcgg gcctcctcag gcattgataa tttgattaat ttcgttggcg
    22141 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
    22201 cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct
    22261 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
    22321 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
    22381 acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc
    22441 agctcgccaa actcgcgatc caattcacgc atctgtaatc cgtaacctcg ctcaataatt
    22501 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
    22561 tgattagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
    22621 ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
    22681 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc
    22741 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
    22801 caatttaccg cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga
    22861 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gtgcagtggc
    22921 aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat
    22981 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
    23041 cggggcaagg cgtttttcca gtggccacgt ctttgagtaa tgctgtccgc ggcgaaacaa
    23101 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
    23161 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
    23221 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
    23281 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
    23341 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
    23401 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
    23461 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
    23521 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
    23581 atgtttccac ctgcctgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
    23641 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
    23701 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
    23761 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
    23821 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
    23881 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
    23941 gactatgccg gaagagtctt acctctacgc cctgccttac aacctgtaca aagagcacgg
    24001 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
    24061 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
    24121 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
    24181 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
    24241 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
    24301 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
    24361 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
    24421 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcactg gtggtatcgg
    24481 tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
    24541 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
    24601 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
    24661 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
    24721 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
    24781 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
    24841 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
    24901 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
    24961 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
    25021 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
    25081 ctggtcccga cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg
    25141 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
    25201 gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc
    25261 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
    25321 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
    25381 caagaatcca gccctctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
    25441 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
    25501 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
    25561 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc
    25621 gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
    25681 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
    25741 tttatggtga acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa
    25801 gttccggttg acgatcacga acgtatcgag aaagttcagg aatacgttgc taactacatc
    25861 aacgctgact ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
    25921 cctgcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg
    25981 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
    26041 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
    26101 gtagaactgg gtgcagggat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
    26161 ggtcgtctgg tcgaactgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
    26221 ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
    26281 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
    26341 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
    26401 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc
    26461 gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc
    26521 tcctactcca ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
    26581 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
    26641 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
    26701 accgtgttta tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
    26761 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac
    26821 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
    26881 tctgcacagc agcagtaacc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc
    26941 ggaaataatc actatttccg gttttttatt ctcttaatct gcattaatcc tttctgatta
    27001 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatttc actttacata cttaccactt
    27061 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga
    27121 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt
    27181 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
    27241 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa
    27301 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
    27361 ctgatgaact tcccgtttga aggattaacc tcaggatcga aatacgggac agctgttggc
    27421 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    27481 attgataacg gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt
    27541 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc
    27601 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    27661 accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    27721 ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    27781 cgcatcgtgg tgtgggttat cgccactatg attggcctga tctttaccat ggtgtacgcc
    27841 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    27901 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    27961 gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    28021 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    28081 ttccgcctta acggcatgac ggttaatacc atggcttcat cctttaccga agggacgcga
    28141 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt
    28201 aatggtgaag cgggtgatgc cagcgtgtta aataccatcc tcaacagcat tgccaatgcc
    28261 attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt
    28321 aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg
    28381 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggcattcca gtttggtgat
    28441 ggcttcagtc acatcattta cccaacctca gcttcgttaa tggcaactct cggtgtgtgc
    28501 agggtggact tccgtaactg gctgaaggtg ggcgcgaccc tacttggact gctgtttatt
    28561 atgtccagcg tcgtagtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    28621 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    28681 ttgcgcttca tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    28741 gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg
    28801 aagggaccgt gagagacgca gctgaacaac gcgccccaga cacggcaatt tttatcttcg
    28861 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    28921 tcgcgacgcg cggattccag cagttgctca tcggcctgaa ccacaccgcc tgcggtcgca
    28981 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    29041 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt
    29101 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    29161 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    29221 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    29281 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg
    29341 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc
    29401 actggcagat gggtatgacc gtacaccagc acatcgttct ggtttaaagc aggtagattt
    29461 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    29521 tgccacggcg cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg
    29581 ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    29641 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    29701 agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac
    29761 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    29821 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa
    29881 acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac
    29941 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    30001 ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca tcgcgaacag
    30061 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    30121 aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat
    30181 ccgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    30241 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    30301 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gcttcaaatg
    30361 ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc
    30421 cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc
    30481 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    30541 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt
    30601 tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat
    30661 ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta
    30721 gacctgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa
    30781 attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgctaag cctctttgag
    30841 tctggtgcca ttttgttgta tctgactgag aaaacaggac tctttttgag tcatgaaacg
    30901 cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    30961 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    31021 cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagcggct ggaaaactcg
    31081 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    31141 gcctggactc gccagcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag
    31201 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    31261 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    31321 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttacgta
    31381 cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    31441 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    31501 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgttgg
    31561 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    31621 aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa
    31681 gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    31741 gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc aaatcacggt
    31801 agtgacgcgt aacccacaga aagccagttc cgttctcggc cctcgggtga cgctatggca
    31861 agggctagcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga
    31921 accgattgct gataaacgct ggactcacga gcaaaaagag cgtctctgcc aaagccgctg
    31981 gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc cgtcggtatt
    32041 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    32101 ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg
    32161 ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc
    32221 ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat
    32281 tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca acggcattct
    32341 ctggctgctg gataacgacc tgcgcgggcc atttaatatg gtttcgccct acccggtacg
    32401 caatgaacaa tttgcccatg cgctcggtca agcgctgcat cgcccggcca ttttgcgcgt
    32461 ccccgcaacc gccattcggc tgttaatggg cgaatcttca atactggtat taggtggaca
    32521 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    32581 agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact
    32641 cccggtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac
    32701 catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc
    32761 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    32821 tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg
    32881 ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    32941 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    33001 atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg
    33061 caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg
    33121 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    33181 aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat
    33241 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccagct tactttggtg
    33301 ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    33361 ggaataatat tgccgcaggt cttcatcaat aaaactgttt gattctagtt taagcgtcgt
    33421 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    33481 gt
//
LOCUS       DAJVYM010000029        33468 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVYM010000029 DAJVYM010000000
VERSION     DAJVYM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.029"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..2074)
                     /gene="acrF"
                     /locus_tag="OQS60_001302"
                     /pseudo
     CDS             complement(1..2074)
                     /gene="acrF"
                     /locus_tag="OQS60_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2086..3243)
                     /gene="acrE"
                     /locus_tag="OQS60_001303"
     CDS             complement(2086..3243)
                     /gene="acrE"
                     /locus_tag="OQS60_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS2387101.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            3642..4304
                     /gene="acrS"
                     /locus_tag="OQS60_001304"
     CDS             3642..4304
                     /gene="acrS"
                     /locus_tag="OQS60_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS2387102.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4307..4486)
                     /locus_tag="OQS60_001305"
     CDS             complement(4307..4486)
                     /locus_tag="OQS60_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS2387103.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(4570..5454)
                     /gene="yhdJ"
                     /locus_tag="OQS60_001306"
     CDS             complement(4570..5454)
                     /gene="yhdJ"
                     /locus_tag="OQS60_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS2387104.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(5540..5836)
                     /gene="fis"
                     /locus_tag="OQS60_001307"
     CDS             complement(5540..5836)
                     /gene="fis"
                     /locus_tag="OQS60_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS2387105.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(5862..6827)
                     /gene="dusB"
                     /locus_tag="OQS60_001308"
     CDS             complement(5862..6827)
                     /gene="dusB"
                     /locus_tag="OQS60_001308"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS2387106.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7156..8037)
                     /gene="prmA"
                     /locus_tag="OQS60_001309"
     CDS             complement(7156..8037)
                     /gene="prmA"
                     /locus_tag="OQS60_001309"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS2387107.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8049..9500)
                     /gene="panF"
                     /locus_tag="OQS60_001310"
     CDS             complement(8049..9500)
                     /gene="panF"
                     /locus_tag="OQS60_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS2387108.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(9490..9732)
                     /locus_tag="OQS60_001311"
     CDS             complement(9490..9732)
                     /locus_tag="OQS60_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS2387109.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(9841..11190)
                     /gene="accC"
                     /locus_tag="OQS60_001312"
     CDS             complement(9841..11190)
                     /gene="accC"
                     /locus_tag="OQS60_001312"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS2387110.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11201..11671)
                     /gene="accB"
                     /locus_tag="OQS60_001313"
                     /gene_synonym="fabE"
     CDS             complement(11201..11671)
                     /gene="accB"
                     /locus_tag="OQS60_001313"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS2387111.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(12649..13623)
                     /locus_tag="OQS60_001314"
     CDS             complement(12649..13623)
                     /locus_tag="OQS60_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2387112.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            13775..15715
                     /gene="csrD"
                     /locus_tag="OQS60_001315"
     CDS             13775..15715
                     /gene="csrD"
                     /locus_tag="OQS60_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS2387113.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16020..17063
                     /gene="mreB"
                     /locus_tag="OQS60_001316"
     CDS             16020..17063
                     /gene="mreB"
                     /locus_tag="OQS60_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS2387114.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17129..18232
                     /gene="mreC"
                     /locus_tag="OQS60_001317"
     CDS             17129..18232
                     /gene="mreC"
                     /locus_tag="OQS60_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS2387115.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18232..18720
                     /gene="mreD"
                     /locus_tag="OQS60_001318"
     CDS             18232..18720
                     /gene="mreD"
                     /locus_tag="OQS60_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS2387116.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            18729..19322
                     /gene="yhdE"
                     /locus_tag="OQS60_001319"
     CDS             18729..19322
                     /gene="yhdE"
                     /locus_tag="OQS60_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS2387117.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19312..20781
                     /gene="rng"
                     /locus_tag="OQS60_001320"
     CDS             19312..20781
                     /gene="rng"
                     /locus_tag="OQS60_001320"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS2387118.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            20849..24649
                     /gene="yhdP"
                     /locus_tag="OQS60_001321"
     CDS             20849..24649
                     /gene="yhdP"
                     /locus_tag="OQS60_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS2387119.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            24805..26250
                     /gene="tldD"
                     /locus_tag="OQS60_001322"
     CDS             24805..26250
                     /gene="tldD"
                     /locus_tag="OQS60_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS2387120.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26378..27243)
                     /locus_tag="OQS60_001323"
                     /pseudo
     CDS             complement(26378..27243)
                     /locus_tag="OQS60_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27426..27629
                     /locus_tag="OQS60_001324"
     CDS             27426..27629
                     /locus_tag="OQS60_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS2387121.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            27637..28569
                     /gene="aaeA"
                     /locus_tag="OQS60_001325"
     CDS             27637..28569
                     /gene="aaeA"
                     /locus_tag="OQS60_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS2387122.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            28575..30542
                     /gene="aaeB"
                     /locus_tag="OQS60_001326"
     CDS             28575..30542
                     /gene="aaeB"
                     /locus_tag="OQS60_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS2387123.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            30634..30906
                     /locus_tag="OQS60_001327"
     CDS             30634..30906
                     /locus_tag="OQS60_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS2387124.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(30962..31225)
                     /gene="yhcN"
                     /locus_tag="OQS60_001328"
     CDS             complement(30962..31225)
                     /gene="yhcN"
                     /locus_tag="OQS60_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS2387125.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(31590..32060)
                     /gene="argR"
                     /locus_tag="OQS60_001329"
     CDS             complement(31590..32060)
                     /gene="argR"
                     /locus_tag="OQS60_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS2387126.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            32495..33433
                     /gene="mdh"
                     /locus_tag="OQS60_001330"
     CDS             32495..33433
                     /gene="mdh"
                     /locus_tag="OQS60_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS2387127.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8132 a         8305 c         8767 g         8264 t
ORIGIN      
        1 cagcccagcc tgatcaatta actcaaagtc gaaacccgtt gccgtgccca gttcaacaat
       61 ggctggcata ttgaatggaa tgacaaaacc gtcgcggatc ttgcccaatt ccattttggc
      121 acgatggatt accgcttccg cactgttttc gtcaccacta cgctcttccc acggtttcag
      181 actgacgaaa gccataccgg cgttttgtgc ctggccgctg aagctaaagc cgttaaccgt
      241 aaagacactt tctacgttcg ctttctcgtt attcagatag taatccgtca cttgatccaa
      301 cactttttgc gtccgctctt gcgtcgcgcc agcgggtaac tgaatcatgg tcagaaagac
      361 accctgatcc tcttcaggta agaaggaaga aggaagacgg aagacgtaaa aacaacacca
      421 ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggaa ccgaggattt
      481 tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta aaccagccga
      541 agaaaccgcc cttattttcg tgatgctcag cagagacggg tttaagcagc gttgcacata
      601 acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa acgatggtga
      661 tagagaactg gcgataaatt gccccagtag aaccgccgaa gaatgccatc ggaataaata
      721 ccgctgacag caccatcgcg atacccacca gtgcgccctg aatttgcgac atcgattttt
      781 ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg ttctccacca
      841 ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac atcgttagtg
      901 tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac accacgggta
      961 ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac atcaccagga
     1021 acaccagcat aatggcttcg aacagcgttt ttaccacttc gtgaatagaa agctggacga
     1081 atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat ggctgtaatt
     1141 ccgccagttt tgccttaatg gctttcgcgg tattgagagc gttcgcgccg gttgctagct
     1201 taatccccag gcccgccgcc ggttttccgt tgatacgagc gataacgtta tagttttcac
     1261 cgccaagttc aactcgtgcg acatctttca ggcgtaccac cgagccgtca ctgtttacgc
     1321 gcagggttac tttgccgaat tcttccggat tttttaaccg cgtctgagca ataatcgagg
     1381 cgttcaactg ttgccctggt aacgctggcg ttccgcccaa ctgcccggca gcgatctgat
     1441 cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat ttgtttagca
     1501 gatcggcgtc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga
     1561 cgccattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc gagatatcgt
     1621 cctgtgtggt gtctgggtta tcagagacaa agcccgccac catcaaatag ctgctactgg
     1681 acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct
     1741 ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac tggaaggtaa
     1801 gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc aggttatcga
     1861 taccattcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag
     1921 cacctggata gtttgcctaa acagaaaccg caggcggtgc aatggttgga tactgagcga
     1981 cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc agcacccatg
     2041 caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt
     2101 atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt tttgcaggcc
     2161 gctgacaatg acttgatcgc cagatttcag accttcgcta atcaaccatt tatcgccaat
     2221 cgcctgactg gcaacgacag ggcgcgcttc cacctgactt ttatcgttaa caatcagcac
     2281 ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggataagaa tggcgtcagg
     2341 ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg
     2401 gttagggaag acagcacgta gggttatgga gccggtgcat tcatcaacgg tcacatcaga
     2461 gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca actctacgtt
     2521 gctggtggcg ttttccttat gcaaatttcc ttgctctacg gattgcttca gcctcataaa
     2581 atcgttgctg gattgggtca catcaacgta gataggatcg agctgctgga cagtcgccag
     2641 ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acggtcgatt tgccgatacg
     2701 tccgctaatt ggcgcagtca ctttggtata agcgagattg atgcgagcgc tttcgactgt
     2761 ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca tcagcaatgg cctggtcgta
     2821 ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta ccgtcaaatg
     2881 cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt cataatttgc
     2941 ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgtacat cgctgccttc
     3001 agtgaaatta cgattcagta cgatcccgct aacctgtggg cgaacttcgg ctatacggta
     3061 agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt tcacaatatg
     3121 aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt tacaaccgac
     3181 gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg catgtttcgt
     3241 cattactatt cctcaaaaaa acaaaagcgc gttatttacc cagaaaggca acgcgttaac
     3301 tcgcagaaag aaaaatacag tttgctatcc tacaaattat cattcgtcga tgtaaggaat
     3361 agttatgaat actggcgtct caaggcacat aaacacaaaa aaagattaat attctactgt
     3421 tttattttga cgcgggttga aagaggcaga attaaaacct cgtaaattga aatatatatt
     3481 catgtggcga atgtatttta ggtaaataat atatattatt tatttgacga tttatagcaa
     3541 ctcaatgtta ccctctcgta taaatacaca ttagatgata gattaacctt cgctattttc
     3601 tcaccctgtg tcgaatatat ttatttcctg aataattaat catggcaaaa agaaccaaag
     3661 ccgaagctct gaagacccgg caagaactga ttgaaactgc catcgcccag tttgcgcaac
     3721 atggcgtaag caagacgacg ttcaacgaca ttgccgatgc cgctaacgtt acgcgtggcg
     3781 ctatctactg gcacttcgag aacaagaccc aactgtttaa tgagatgtgg ttgcaacagc
     3841 cttcattgcg ggagttaatc caggaacact tgacggctgg attagagcat gacccgtttc
     3901 aacaattgcg tgaaaaattg attgtcggct tgcaatatat tgccaaaatt ccccgccagc
     3961 aggcgttgct gaaaatctta tatcacaaat gtgaatttaa tgatgggatg ctggccgagg
     4021 gagtgatacg cgaaaagatg ggctttaatc cgcagactct ccgcgaagta ttgcaggcgt
     4081 gtcagcaaca aggttgtgta gcaaataacc tcgatttaga tgttgtgatg attattattg
     4141 atggtgcctt cagcggaatt gttcaaaact ggttaatgaa tatggcgggt tatgatcttt
     4201 ataaacaagc acccgcgctg gtcgataacg tattaagaat gttcatgcca gatgaaaaca
     4261 taacgaaatt aattcatcaa acaaatgaat taagtgtcat gtaaattcac attctgtcct
     4321 gaaaattcag ttcgtcacag ttaaccagtt ttagtggata aactgagttc atattacggc
     4381 atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta tcaaacagaa
     4441 aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc tgagcctgtg
     4501 catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa aaccaggctt
     4561 acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc ttcgttttga
     4621 caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt aatgcgacgc
     4681 gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa taccaatgaa
     4741 ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag
     4801 aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg cttccggttt
     4861 ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa aatcccagac
     4921 gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat aatcgatcaa
     4981 cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga atgtgtagtt
     5041 ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt gttttttcgc
     5101 ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa aaagcttgcg
     5161 gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca tgctgccctg
     5221 ctttttcaga acgcggtggc actctacaat cacttcaaac agccagtcga taaacagatc
     5281 ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg gtgggtcggc
     5341 aaagatcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaggg catcaccgtg
     5401 aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc tcatggtcac
     5461 tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc tttttaatca
     5521 agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta cgcagcgtac
     5581 cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg tattgcatca
     5641 ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg tcattcacat
     5701 cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag tcacgcaggg
     5761 gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg tcagaattta
     5821 cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa attttcgaag
     5881 tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga
     5941 aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg
     6001 tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc
     6061 ttaacctctg ccaaaggcag cgggggcagc aactccccag tgtccagata atgctggatt
     6121 tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc atccgcccct
     6181 gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc
     6241 ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc tccattgaac
     6301 aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc ttcagccagt
     6361 tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat cttcagggta
     6421 acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac atccgggtac
     6481 tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca acccatattg
     6541 atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc catttctttc
     6601 ggatcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg caccatccgt
     6661 aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc ggatactgtc
     6721 aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg
     6781 ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag ttaagaaatg
     6841 accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca
     6901 aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag cgtggtgaga
     6961 taattactca taaaaatcat catattagaa aatagtgact aaaatttaca ctcaaagaaa
     7021 tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa atttgcgtaa
     7081 ataaccggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct
     7141 acggcgatgc gaaggttaat tcttacgacc ggtaatgcgg caccactctt ctttttccac
     7201 gaccgggtcc agtgcgaagc tgtcgacata agcttcacaa acgctctctg cctggcttgc
     7261 cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc
     7321 cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt tcatttcttc
     7381 tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac gttcggcgtt
     7441 gtcgcggcta gcctgaatcg cctgcggatc gatatcaata ccaatggctt ttgctgcacc
     7501 cagtttcagc gccgcgatag ccagaatgcc ggaaccacag ccaaagtcga tgactatttt
     7561 accggttaaa tcgaggctgt cgagccactg caggcacaga gaggtagttg gatgggtacc
     7621 cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt ccggcacatc
     7681 acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga aattatccat
     7741 ccattcgcgc tcccagtctt tatcttccag ttgttcgatt ttatgcgcga agcctgcgcc
     7801 gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa
     7861 cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg gctcaaatac
     7921 tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca tcagcgcatc
     7981 gctaagatct tcagcgttcg cgccggtggt gttcagtttc agttggatcc aaggcatggc
     8041 aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg
     8101 tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg gtggaagccc
     8161 aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac gatcatcgca
     8221 cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag cggccacagg
     8281 aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat ttctggcggc
     8341 ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc cgacatccgc
     8401 ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag atctttaatg
     8461 atcgtagcgg aactttgtag caattgggcg ttaattgtcg acatgatcgc agccataggt
     8521 gcagccagaa agatcccggc agcaaacggt ggcagcactt ttaccattaa cgtcgggatc
     8581 accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc ggctaggtgc
     8641 ataccgaaca tcaggattgc gactacaatc gtaccgatga tgatcccccg gtgtaccgct
     8701 ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat cacgccaaag
     8761 cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt
     8821 ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct taagccgcca
     8881 gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac aagcccttgc
     8941 atggtgtcgt tcagcacgct ggcgcgaaag ccgccaaagg cggtatacaa cgcaatgctg
     9001 ataccgaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc
     9061 gcaccgccga taaactgcac ggtcattgcc ccaacgaacg caaccagcaa actcaaactc
     9121 gccagccaca ccagaagacg actctggtag cgggcaaaca gcatgtcgtt cagcgtcact
     9181 gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag tgaaagccag
     9241 actgcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt ataagcagct
     9301 cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt catcgccagc
     9361 acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt gccggtgctc
     9421 cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc gaccagcggt
     9481 agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga taaatttcac
     9541 catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca tctcaaacca
     9601 gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcggcta ctaaccaaac
     9661 tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa
     9721 acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac cttatggggg
     9781 ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttggcgc ttttgcagtc
     9841 ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc caccatgctg
     9901 gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga taccgtcgat
     9961 gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta
    10021 gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc ccgcgtagat
    10081 atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg
    10141 gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg catggccgcg
    10201 aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac ggagctgttc
    10261 tttgatcagg tcaacgccag tgatcatttc ggtaaccggg tgttctacct gaatacgggt
    10321 gttcatttcg atgaaataga actcgccgtt ttcgaacaga aactcgaaag tacctgcacc
    10381 gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc gacgcagttc
    10441 cggggtaatg cccggtgctg gcgcttcttc gaccactttc tggtggcggc gctgcatgga
    10501 gcagtcacgt tccgccagat agatagcgtt gccccgaccg tcagccagta cctgaatctc
    10561 gacgtggcga ggattttcca ggtatttctc catgtaaacc atatcgttgc tgaaagcagc
    10621 tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac
    10681 gcgcataccg cgaccaccgc cgccgccgga ggctttgata atcaccggat aaccaatgcg
    10741 tttagcaatg gcacggtttt tatccatatc gtcgcccaac gggccgtcag aacccggtac
    10801 gcaagggacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc ccatcaggcg
    10861 aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct gctcggcaaa
    10921 gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc cggtgatttc
    10981 agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag cagggccaat
    11041 acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac
    11101 agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg caatctcgcc
    11161 gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg acgaccagcg
    11221 gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg gtaccggatt
    11281 tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg gtatcgccca
    11341 cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga
    11401 aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct gctggcgctt
    11461 ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc tgcatcattg
    11521 gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca cggctaatgc
    11581 gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct tcaaccagct
    11641 cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt
    11701 gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata
    11761 gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtat caatgttaga
    11821 cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa aatatacctt
    11881 cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc
    11941 acattataac gatttcgtag caattggcag ctaaatactg gtctcatcag ggaagataat
    12001 caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc
    12061 acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa gctgtaaagc
    12121 cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca aataatgtag
    12181 ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg gattacccaa
    12241 ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca accgactgac
    12301 aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa tcagattatt
    12361 caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc
    12421 ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag tatcaatgcg
    12481 catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt gtacaacttc
    12541 ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta accgattgaa
    12601 taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt agttaacctt
    12661 caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt tcggtgcctc
    12721 tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat cggcgaccag
    12781 tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa
    12841 acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac cacccgccag
    12901 accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg ccagcacttt
    12961 gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac gggattcggc
    13021 aaactcatca cgagggagaa tacggctggc acctaaactt ttcagatatt catgggtact
    13081 ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg ccacggcggt
    13141 actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc
    13201 ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac cgataatcat
    13261 tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc
    13321 ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga gtaacacctc
    13381 ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc
    13441 aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt
    13501 atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca ggcgactttc
    13561 gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg
    13621 cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagat tatactcgct
    13681 aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga tttgctagta
    13741 tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga aattttcggc
    13801 ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct gttcgctaag
    13861 tttctacaac gcaattcagt ataagtttag tcatcgcgtt caggcggtgg cgacggcgat
    13921 tgatacccac cttgtgtcga atgacttcag cacattaagg ccacaaatta ccgaattaat
    13981 gatgtcggca gatatcgttc gtgtagacct gctccatggt gataaacagg tttataccct
    14041 ggccagaaat ggtagttatc gtccggttgg ctccagcgat ctgtttcgcg aactgagtgt
    14101 tccgttgata aagcatccgg ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa
    14161 ctatttccat tctttaatga ctaccgcgcc gctcacgggg gcgattggct ttatcattgt
    14221 tatgctcttc ctggcggtac gctggttaca acggcaactt gccgggcaag aattgctgga
    14281 aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg gaaccatcta
    14341 tgaatggccg cccagaacca gcagtgcgct agatacgctg cttcgtgaaa ttcagaacgc
    14401 acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgccc aggacgtgaa
    14461 aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac tggaagatca
    14521 ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt
    14581 gagcgatact tgggggcaca gccaggttga agaacagttc ttcactctga ctaatctgct
    14641 gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc gcagtgattt
    14701 tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatcgccg gtcagttaat
    14761 caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata tgatccacat
    14821 tggtatctgc gcctggcgta gtggtcagga taccgagcag gtaatggaac atgcagagtc
    14881 tgccacgcgt aatgcgggat tgcagggcgg caatagctgg gctatttacg atgactcgtt
    14941 gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg
    15001 cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg
    15061 cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc
    15121 gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat
    15181 tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg ttgagtcgct
    15241 gattcgcccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca
    15301 acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata tcagtcgttt
    15361 acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca accaggctgg
    15421 tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac tcaagctcca
    15481 tccggggctg gtcagaaaca ttgagaagcg aacggagaac cagctgctgg ttcaaagcct
    15541 ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag
    15601 cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc
    15661 ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg tttaacctgc
    15721 cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct
    15781 gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt gcaaacaggc
    15841 gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg taacaaagga
    15901 aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg ctgctgcgtg
    15961 tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta tcccttagta
    16021 tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg ggtactgcga
    16081 ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc gtggtggcca
    16141 ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc
    16201 agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa gacggcgtta
    16261 tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa gtgcacagca
    16321 acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc gcgacccagg
    16381 ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga
    16441 ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt
    16501 ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg
    16561 tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct atcatcaact
    16621 atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt atcaagcacg
    16681 aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc
    16741 tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc gaagcactgc
    16801 aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag tgcccgccgg
    16861 aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc gcactgctgc
    16921 gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtagttgtt gctgaagacc
    16981 cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac atgcacggcg
    17041 gcgacctgtt cagcgaagag taatcggatg caggcagggg aaatgtctgt ttaccctgcc
    17101 tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg gcccgtcgct
    17161 acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg ccgacagccg
    17221 cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc ctttctactt
    17281 tgtttccaat gctcctcgtg aattgctgga tggcgtatcg cagacgctgg cctcgcgtga
    17341 ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa acagtgaact
    17401 gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc
    17461 gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta acgatcctta
    17521 tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag gccagccggt
    17581 catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt
    17641 gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca acgatatccg
    17701 cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc tgccagcgaa
    17761 tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc gtttcccgga
    17821 aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg cttatactgt
    17881 gattcaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc tgctgtgggg
    17941 cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg ttgctaatga
    18001 acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt
    18061 acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccgacga caggaaatgc
    18121 agctactgcg cctgctgcgc ccacacagcc tgctgctaat cgctctccac aaagggctac
    18181 gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc
    18241 tatcgtagcc agggacgttg ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa
    18301 atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt actcatcttg
    18361 ttgtattgga tcctggcctt gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt
    18421 gccatactgg atctgatcag cggctcgacg cttggcgtac gcgtattggc gatgagcatc
    18481 attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt atggcagcag
    18541 gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg ggcagagttt
    18601 ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt caatggggtg
    18661 ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa
    18721 aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag gagttacttg
    18781 cgcaacttgg tgtgaccttt gaacgtattg ttacgggcat tgaggagcag cgtcagccgc
    18841 aggagagcgc gcagcagtat gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg
    18901 cgcaaacggc gcaggatctc ccggtgctgg gtgcggatac tatcgttatc ctgaatggag
    18961 aagtgctgga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc aaattatcgg
    19021 gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac attctcgatt
    19081 gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc gcgggctatg
    19141 tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg ctgggtggct
    19201 gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttacct ctggttgaaa
    19261 cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa catgacggct
    19321 gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga tggcggtatt
    19381 ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag
    19441 ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat tgggctggat
    19501 aaagccgcgt ttcttcatgc atccgatatc atgccgcaca ccgaatgtgt ggcgggtgaa
    19561 gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg
    19621 atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac caccgatatc
    19681 acgctgcctt ctcgctatct ggtctttatg ccaggggctt ctcacgttgg ggtttcccaa
    19741 cgtattgaaa gcgaatcaga acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac
    19801 gagcagggcg ggtttatcat ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc
    19861 tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg
    19921 cagacccgtt atcagctgta cggcgaactg gcgctggctc agcgtgttct gcgtgatttc
    19981 gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga agcattactt
    20041 gagttcacct cggagtacat tcccgagatg accagcaagc tggagcatta caccggacgc
    20101 cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa
    20161 gtagaactga aatccggtgg ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg
    20221 gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacaccat tttcaatacc
    20281 aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct gggcgggatt
    20341 atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt gctgcactcg
    20401 ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg
    20461 gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac
    20521 gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa
    20581 atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt cctggtctat
    20641 gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct ggcggaagtg
    20701 gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa ccaggagcag
    20761 tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg gtttttgagt
    20821 cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt tactgcttac
    20881 tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta ttgctttacc
    20941 gcatcttgac gcctggcgtc cggaaatcct caacaaaata gaatccgcga ctggcatgcc
    21001 ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc ttgaagcaca
    21061 cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg ttactctggc
    21121 gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc tcactttctg
    21181 gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg acagtctgga
    21241 agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc tccgcgacag
    21301 tgaagtcagt ttcctgacgc catccggtca gcgtgccgag ctggcgatcc cacaactcac
    21361 ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct ccagccttac
    21421 cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag
    21481 caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc tcggtaaatg
    21541 gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct ggatgacgat
    21601 cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt
    21661 gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata ttacgcgtga
    21721 aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg gcaaaccctg
    21781 gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg gcaaagacaa
    21841 taaacgcagt gacgaactcc ggattcgcgc cagtaatctg gagctggcag gcctggaggg
    21901 catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc gctccacaca
    21961 accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg cagacaagac
    22021 ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat taccgggtgc
    22081 ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat
    22141 gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa tcgccgacgg
    22201 ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg ggcgtaatat
    22261 tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc aacctgctaa
    22321 cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt cacaagcctg
    22381 gcactatttc ccggaaaact tgatgggtaa agacctggtt gattacttaa gtggcgcgat
    22441 tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc aactcttccc
    22501 ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc
    22561 cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact ttattaacga
    22621 cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac
    22681 cgcagtgatc cctgactact cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc
    22741 gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc tgggtgcgac
    22801 cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg atatcccgct
    22861 gaacggcgag ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca gtctgtttat
    22921 caaaccactc gacagcaccc tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga
    22981 tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg tggatttttc
    23041 caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta actggcaacc
    23101 ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg
    23161 ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata acgtagagct
    23221 gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca aacctgcggg
    23281 tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg aattaaccgg
    23341 acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa agctgacgct
    23401 cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg
    23461 cgttgaactc aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg
    23521 cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa tacataacgt tacgtacgcc
    23581 tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc aaccgacggc
    23641 aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa
    23701 taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg tggcgaaaac
    23761 tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta acttccgtgg
    23821 ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa aattcggtcg
    23881 cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg gactgattga
    23941 tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg
    24001 tacctcgctg aaaggaaaac tgcgcgggca gaaaattgat gccgccgcag aattttttgg
    24061 tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac actggcgcaa
    24121 agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc aactgggtaa
    24181 aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct tattgagcgt
    24241 agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg aagggttcta
    24301 ttttgactcc attcgcagca ccgcgtggat taaagacggg gttatgcaca ccgacgacac
    24361 gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg
    24421 cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc
    24481 ggcttttgcg gttaacccca ttgttggcgc ggcagttttt gccgctagta aagtgctggg
    24541 gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc tggacgatcc
    24601 gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg
    24661 cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag ccagaacata
    24721 accgtaggcc ggatagggcg ttcatgccgc atccggcagc cgtaaaaaat cctctactgc
    24781 agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca attgctagcg
    24841 gcgaacagcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc
    24901 cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt tttagaagac
    24961 cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc
    25021 ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct ggaacagagt
    25081 gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc
    25141 gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa
    25201 gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta
    25261 caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc ggccaccgac
    25321 ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa
    25381 gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc
    25441 ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt
    25501 atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt
    25561 ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct ggaaggcgac
    25621 ttcaaccgcc gcggcacttc agtatttagt gggcaggtcg gggagctggt ggcttcagaa
    25681 ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat
    25741 gacgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct gaaaggctac
    25801 atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt
    25861 gaatcctacg cccatctgcc catgccgcgt atgactaaca cctatatgct gccgggtaaa
    25921 tcgaccccgc aggaaattat tgaatccgtt gagtacggta tctatgcacc gaactttggt
    25981 ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga agcatatctg
    26041 attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc
    26101 gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa cggcgtgggt
    26161 gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac gctgaaagtt
    26221 gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa ggataattaa
    26281 tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag gggaacccgt
    26341 tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc ccgcgcccgt
    26401 gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag ttgatcacga
    26461 cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc gggcgtggat
    26521 ctgactgata acgcggcagc aggatctcca gctcaccgcg attgatctcg ttgatcaccc
    26581 acatcagcgg cacgtaggcg atcccggcac ccgccgtcag ccagcgcacc agcgtcatcg
    26641 gatcattagt cacaaatctt ccttgtggga tcaggcgagt cgagatccct tccggtgcga
    26701 tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatga ctactcaaat
    26761 cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg cacaccacca
    26821 tcggcatcgc gcccagacgg cgggaaaaca ggctggaatt ccggtaacca gattgacgct
    26881 caaacctggg tattctttca gcatttttgc tgtcagcccg gcgagaacat tttgtgccat
    26941 agttgaagaa cagccaatgc gcagcgtccc gatgggggtg ttattgaagg catacagttg
    27001 ctcatgaaca tcctgcactt catgaagcat ccgacggcag ccctggtagt aaattctgcc
    27061 ggcttcggtc aggccaatgc tgcgtgtgct acggtttaac agctttacct gcaactcatc
    27121 ttccagtttt gacaccgtct gactgatgga cgaaacgctc atctgtagct gtctggcggc
    27181 ggcggtaaaa gagccaaatt caactacttt ggcaaacacc gacatgcgtt ttagtcgttc
    27241 cattattcac tctgacttaa aagtgattta gatcacataa tatagataac agcataacag
    27301 ttacgctaat atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc
    27361 ccttctctgc ggcgacagat gctgaaaata ataacgcctg ctctctcttt acaaccaagg
    27421 tcaacatgag tctgtttccc gttatcgtgg tgtttggact gtccttccca ccgatatttt
    27481 ttgaattgct tttatcactg gcgattttct ggctggtgcg ccgggtactt gtgccaacag
    27541 gtatctacga ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt
    27601 tttatttgat atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc
    27661 tcccgtacgg ccatcacggt cgtattagtc attctagcct tcatcgcaat ttttaatgcc
    27721 tgggtctatt acaccgaatc cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt
    27781 gcgatcgcgc cggacgtttc tggactcatt acccaggtga atgttcatga taaccagctg
    27841 gtgaaaaaag gacagatact gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag
    27901 gaagcgcaag ccgatgttgc ttattatcag gtactggcac aggagaaacg ccaggaggcc
    27961 ggacgtcgta accgtctcgg tgtgcaggcg atgtctcgcg aagagatcga ccaggccaac
    28021 aacgtactac aaacggttct gcatcagtta gcgaaagcgc aggcgacccg cgatctggca
    28081 aaactggatc ttgaacgcac ggtgatccgc gcgccagcag atggctgggt gaccaacctc
    28141 aacgtctata ccggtgagtt tattactcga ggatcaacgg cggttgcgct ggtgaaacag
    28201 aactccttct atgtactggc ctatatggaa gaaactaagc tggaaggggg gcgtccgggg
    28261 tatcgtgcag agatcacgcc gcttggcagt aacaaagtgc tgaaagggac tgttgatagt
    28321 gttgccgcag gggtcaccaa cgccagcagc acgcgtgacg acaaagggat ggcgactata
    28381 gactctaatc ttgaatgggt gcgtcttgcg caacgtgttc cggttcgtat tcgtctcgac
    28441 aaccagcaag agaacatctg gcctgcgggc accactgcta cagtggtggt cactggcaaa
    28501 caagatcgcg acgaaagcca ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag
    28561 tttggttaat cacgatgggt attttctcca ttgctaacca acatattcgc tttgcggtaa
    28621 aactggcgac cgccattgta ctggcgctgt ttgttggctt tcacttccag ctggaaacgc
    28681 cacgctgggc ggtactgaca gcggcgattg ttgccgccgg tccggccttt gctgcgggag
    28741 gtgaaccgta ttctggcgct attcgctatc gtggcttttt gcgcatcatc ggcacattta
    28801 ttggctgtat tgccggactg gtgatcatca ttgcgatgat ccgcgcacca ttattgatga
    28861 ttctggtgtg ctgtatctgg gccggttttt gtacctggat atcctcgctg gtacgaatag
    28921 aaaactcgta cgcgtggggg ctggccggtt ataccgcgct gatcattgtg atcaccattc
    28981 agccggaacc attgcttacg ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg
    29041 gtattgtgtg tgcgattatg gcggatttgc tcttttctcc gcgatcgatc aaacaagaag
    29101 tagatcgaga gctggaaagt ttgctggtcg cgcaatatca attaatgcaa ctctgtatca
    29161 agcatggcga tggtgaagtt gtcgataaag cctggggcga cctggtgcga cgcaccacgg
    29221 cactacaagg catgcgcagc aacctgaata tggaatcttc ccgctgggcg cgggccaatc
    29281 gacgtttaaa agcgatcaat acgctatcgc tgacgctgat tacccaatcc tgcgaaactt
    29341 atcttattca gaatacgcgc ccggaattga tcactgatac tttccgcgaa ttttttgaca
    29401 cgccggtaga aaccgcgcag gacgtccaca agcagctcaa acgcctgcgg agagttatcg
    29461 cctggaccgg ggaacgggaa acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc
    29521 gttatcagct tctcaagcgc ggcgttatca gtaacacaaa aatcaacgcc accgaagaag
    29581 agatcctgca aggcgaaccg gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg
    29641 ttaacttctg gcgaaccaca ctttcctgca ttctgggcac gcttttctgg ctgtggacgg
    29701 gctggacttc cggcagtggt gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc
    29761 gtttgccgaa tccacgcatg gtggcgatcg actttatcta tgggacgctg gccgcgctgc
    29821 cgttagggct gctctacttt ttggtgatta tccctaatac ccaacagagc atgttgctgc
    29881 tgtgtattag cctggcagtg ctgggattct tcctcggtat agaagtacag aaacggcgac
    29941 tgggctcgat gggggcactg gccagcacca taaatattat cgtgctggat aacccgatga
    30001 ctttccattt cagtcagttt ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg
    30061 cgttcaccgt tattttgctg gtgcgggata aatcgcgcga caggactgga cgtgtactgc
    30121 ttaatcagtt tgtttttgcc gctgtttccg cgatgactac caatgtggca cgtcgtaaag
    30181 agaaccacct cccggcactt tatcagcagc tgtttttgct gatgaataag ttcccagggg
    30241 atttgccgaa atttcgcctg gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg
    30301 cgccgatccc ggttaacgag gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag
    30361 accatgtgat atctgcccgt agcgatgata aacgtcgtcg gtactttggc cagttgctgg
    30421 aagaactgga aatctaccag gaaaagctac gcatctggca agcgccaccg caggtgacgg
    30481 aaccggttca tcggctggcg gggatgctcc ataagtatca acatgcgttg accgatagtt
    30541 aagtcaaaac cgacgccaaa agcgtcggtt ttttcatggc tatacttagc gatgcacggc
    30601 agaactcgcc gcgaaacgtg acggtggcaa cagatgaata tttatacctt tgattttgat
    30661 gagattgaga gtcaggagga tttttatcgt gactttagcc aaacctttgg tctggctaaa
    30721 gataaggtac gcgatctcga ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta
    30781 ccacttgaga ttgaatttgt tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta
    30841 atattgctgt ttgatgaggc agaggaagag ctggaagggc atttgcgttt taatgttcgt
    30901 cattagcgca aaaaaaagcc cccgaaccgg gggcaatatc gtcggacaag acgatgaggg
    30961 tttatttgta cagttcagcc gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct
    31021 ggtaggccgt tgcgcctttc tcttccgctt ttttgttcag catttcacgc atatccattg
    31081 gcgaagacgc cacaccactt acggatacgg tcccgattgc ttcacgattt tgtgcttgtg
    31141 cagtatcaat ggagtcggca gcgaatgcac cgaaagagag aacagaaagt acgcttaatg
    31201 cagctacagt ggttttgatt ttcatgattt ttacctcgac ataatctttt agctgggcct
    31261 ttgtttcgtg atccatatca caaaatcaag catacactaa ttattgaact aattaatacc
    31321 acactaactg tttcagttga tactaagtgt taaaaatgtg aattttaata acaaaaaaga
    31381 attaaaaaca cggttattta ttgttaattc tagtattttc aattagataa tgaattttac
    31441 ataatgtgct ttttttaccg tatgtattca ttgtgtgaat gatatgtcgc ggtaaaacgc
    31501 actattcgtt aaattaatcg caggggaaag cagggggttg agaggaataa gcaacatttt
    31561 ccccgccgcc agaagcgacg gggcagagat taaagctcct ggtcgaacag ctctaaaatc
    31621 gcttcgtaca ggtctttgac ggtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg
    31681 ccagcgatgg tgcccagaat accttctgct ttgcccagtg agtccagcag gcgagcaatt
    31741 aactgcgccg cgccagggct ggtatgaatc acgacaactg catcgttgta gtcgatatcc
    31801 agcaccagat tcttcaatgg actggaggtg gttggtacac ccagttcagc tggcaggcag
    31861 taaaccattt ccattttggc attgcgtgta cgtacagcac caaacttggt caacatccgc
    31921 gagactttag actgattaat attgtcaaag ccttgctcct gcaacgcggc gacgatttcg
    31981 ccctgggagc taaatttctc ttctttaagt aatgctttaa atgctttaac tagttcttct
    32041 tgcttagccg agcttcgcat aagtcacccg atatggtggt tgatacaaca ttattgtgca
    32101 tacagatgaa tttttatgca aacagtcagc cctgaagaag gctgaaataa tgttatgaaa
    32161 gagcgggatt ttatcaaatt tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt
    32221 ctgtttaaaa gtaaattaat tgttatcaaa ttgatgttgt tttggctgaa cggtagggta
    32281 tattgtcacc acctgttgga atgttgcgct aatgcataag cgactgttaa ttacgtaagt
    32341 taggttcctg attacggcaa ttaaatgcat aaacgctaaa cttgcgtgac tacacattct
    32401 tgagatgtgg tcattgtaaa cggcaatttt gtggattaag gtcgcggcag cggagcaaca
    32461 tatcttagtt tatcaatata ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc
    32521 tggcggtatt ggccaggcgc ttgcactact gttaaaaacc caactgcctt caggttcaga
    32581 actctctctg tatgatatcg ctccagtgac tcccggtgtg gctgtcgatc tgagccatat
    32641 ccctactgct gtgaaaatca aaggtttttc tggtgaagat gcgactccgg cgctggaagg
    32701 cgcagatgtc gttcttatct ctgcaggtgt agcgcgtaaa ccgggtatgg atcgttccga
    32761 cctgtttaac gttaacgccg gcatcgtgaa aaacctggta cagcaagttg cgaaaacctg
    32821 cccgaaagcg tgcattggta ttatcactaa cccggttaac accacagttg cgattgctgc
    32881 tgaagtgctg aaaaaagccg gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct
    32941 ggacatcatt cgttccaaca cttttgttgc ggaactgaaa ggcaaacagc caggcgaagt
    33001 tgaagtgccg gttattggcg gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt
    33061 tcctggcgtt agttttaccg agcaggaagt ggctgatctg accaaacgca tccagaacgc
    33121 gggtactgaa gtggttgaag cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca
    33181 ggcagctgca cgttttggtc tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt
    33241 cgaatgtgcc tacgttgaag gcgatggtca gtacgcccgt ttcttctctc aaccgctgct
    33301 gctgggtaaa aacggcgtgg aagagcgtaa atctatcggt accctgagcg catttgaaca
    33361 gaacgcgctg gaaggtatgc tggatacact caagaaagat atcgccttgg gcgaagagtt
    33421 cgttaataag taattgatta gcgaataata aaaaaccgga gcacagac
//
LOCUS       DAJVYM010000030        33439 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVYM010000030 DAJVYM010000000
VERSION     DAJVYM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.030"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            521..760
                     /locus_tag="OQS60_001331"
     CDS             521..760
                     /locus_tag="OQS60_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS2387128.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(948..1925)
                     /gene="intI2"
                     /locus_tag="OQS60_001332"
                     /pseudo
     CDS             complement(948..1925)
                     /gene="intI2"
                     /locus_tag="OQS60_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2257..2730
                     /locus_tag="OQS60_001333"
     CDS             2257..2730
                     /locus_tag="OQS60_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS2387129.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2825..3349
                     /gene="sat2"
                     /locus_tag="OQS60_001334"
     CDS             2825..3349
                     /gene="sat2"
                     /locus_tag="OQS60_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS2387130.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3407..4195
                     /gene="aadA1"
                     /locus_tag="OQS60_001335"
     CDS             3407..4195
                     /gene="aadA1"
                     /locus_tag="OQS60_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS2387131.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4271..4768
                     /locus_tag="OQS60_001336"
     CDS             4271..4768
                     /locus_tag="OQS60_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387132.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4829..5200
                     /locus_tag="OQS60_001337"
     CDS             4829..5200
                     /locus_tag="OQS60_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387133.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5610..5987
                     /locus_tag="OQS60_001338"
     CDS             5610..5987
                     /locus_tag="OQS60_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387134.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            6010..6162
                     /locus_tag="OQS60_001339"
                     /pseudo
     CDS             6010..6162
                     /locus_tag="OQS60_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6218..7834)
                     /locus_tag="OQS60_001340"
     CDS             complement(6218..7834)
                     /locus_tag="OQS60_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS2387135.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7835..9361)
                     /locus_tag="OQS60_001341"
     CDS             complement(7835..9361)
                     /locus_tag="OQS60_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS2387136.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(9364..11031)
                     /locus_tag="OQS60_001342"
     CDS             complement(9364..11031)
                     /locus_tag="OQS60_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS2387137.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(11028..13136)
                     /locus_tag="OQS60_001343"
     CDS             complement(11028..13136)
                     /locus_tag="OQS60_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS2387138.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(13123..13944)
                     /locus_tag="OQS60_001344"
     CDS             complement(13123..13944)
                     /locus_tag="OQS60_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS2387139.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(14103..15932)
                     /gene="glmS"
                     /locus_tag="OQS60_001345"
     CDS             complement(14103..15932)
                     /gene="glmS"
                     /locus_tag="OQS60_001345"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS2387140.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(16094..17464)
                     /gene="glmU"
                     /locus_tag="OQS60_001346"
     CDS             complement(16094..17464)
                     /gene="glmU"
                     /locus_tag="OQS60_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS2387141.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17815..18234)
                     /gene="atpC"
                     /locus_tag="OQS60_001347"
     CDS             complement(17815..18234)
                     /gene="atpC"
                     /locus_tag="OQS60_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS2387142.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(18255..19637)
                     /gene="atpD"
                     /locus_tag="OQS60_001348"
     CDS             complement(18255..19637)
                     /gene="atpD"
                     /locus_tag="OQS60_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS2387143.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(19664..20527)
                     /gene="atpG"
                     /locus_tag="OQS60_001349"
     CDS             complement(19664..20527)
                     /gene="atpG"
                     /locus_tag="OQS60_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS2387144.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(20578..22119)
                     /gene="atpA"
                     /locus_tag="OQS60_001350"
     CDS             complement(20578..22119)
                     /gene="atpA"
                     /locus_tag="OQS60_001350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS2387145.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(22132..22665)
                     /gene="atpH"
                     /locus_tag="OQS60_001351"
     CDS             complement(22132..22665)
                     /gene="atpH"
                     /locus_tag="OQS60_001351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS2387146.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(22680..23150)
                     /gene="atpF"
                     /locus_tag="OQS60_001352"
     CDS             complement(22680..23150)
                     /gene="atpF"
                     /locus_tag="OQS60_001352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS2387147.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(23212..23451)
                     /gene="atpE"
                     /locus_tag="OQS60_001353"
     CDS             complement(23212..23451)
                     /gene="atpE"
                     /locus_tag="OQS60_001353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS2387148.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(23498..24313)
                     /gene="atpB"
                     /locus_tag="OQS60_001354"
     CDS             complement(23498..24313)
                     /gene="atpB"
                     /locus_tag="OQS60_001354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS2387149.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(24322..24702)
                     /gene="atpI"
                     /locus_tag="OQS60_001355"
     CDS             complement(24322..24702)
                     /gene="atpI"
                     /locus_tag="OQS60_001355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS2387150.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(25319..25942)
                     /gene="rsmG"
                     /locus_tag="OQS60_001356"
     CDS             complement(25319..25942)
                     /gene="rsmG"
                     /locus_tag="OQS60_001356"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS2387151.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(26006..27895)
                     /gene="mnmG"
                     /locus_tag="OQS60_001357"
     CDS             complement(26006..27895)
                     /gene="mnmG"
                     /locus_tag="OQS60_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS2387152.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(28274..28717)
                     /gene="mioC"
                     /locus_tag="OQS60_001358"
     CDS             complement(28274..28717)
                     /gene="mioC"
                     /locus_tag="OQS60_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS2387153.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(28807..29265)
                     /gene="asnC"
                     /locus_tag="OQS60_001359"
     CDS             complement(28807..29265)
                     /gene="asnC"
                     /locus_tag="OQS60_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS2387154.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            29417..30409
                     /gene="asnA"
                     /locus_tag="OQS60_001360"
     CDS             29417..30409
                     /gene="asnA"
                     /locus_tag="OQS60_001360"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS2387155.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(30414..31865)
                     /gene="viaA"
                     /locus_tag="OQS60_001361"
     CDS             complement(30414..31865)
                     /gene="viaA"
                     /locus_tag="OQS60_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS2387156.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31859..33355)
                     /gene="ravA"
                     /locus_tag="OQS60_001362"
     CDS             complement(31859..33355)
                     /gene="ravA"
                     /locus_tag="OQS60_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS2387157.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8519 a         8452 c         7793 g         8675 t
ORIGIN      
        1 tcggcagccc ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
       61 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
      121 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttaac
      181 taatctagct aacctgccgc tcagcttagt acgatcctat atccaactga tcataatcct
      241 ttcatcagta tcccaaatta aatccccctc ctagactgcc tcaccaacac tttacagtga
      301 tcccgtattt ccggacaggt gattgatttt tttcgcttgt acaggtgtta agccacagtt
      361 atcttgatgt ggacattatg cattgtaata gcccattaac atgcgctaag agcataaatt
      421 aatatacagt aacaagcatt acgaggtgct aatgtttgga aagcagcctc ttccaactac
      481 aataataagt taactatcct ccacccacaa ataattacaa atgacccctt tccgctataa
      541 ctccgatctc accagcggct cgctacaaac ccgagagtgt cgcattatta ctggcttgtt
      601 actccaagag cttgatgaag ctgcctggga taaagccatg tataaggaaa atgtgcttca
      661 gaaacgcacg caatctacgg taagacgtat ttcttcagcg cttagaaaac gcctagaaca
      721 cctaagctct gacttttggg cttttgcgtt tttatgttag gcattgtctg gcaactggag
      781 gaagtgtaat gaccatgaat ggtcactgtg attgacagta tcttgcttaa acttatctga
      841 acaatggtag aactggctca tatttatccc ctctatgatt gtttatcttt ttcaccataa
      901 aaagtatcag gggctactca gggctacata aagatattta tctactttta ctgattgata
      961 agtagcatca gtccatccgc aggactggtg gtgccggcaa aatgctgacc caacacatgc
     1021 gtatagattt gcgtggtctt aacatcgtta tgccctaaga gttcttgcac agtgcgaata
     1081 tcacgccccg cttgtaatag atgcgtagca aacgagtgac gaaatgtatg acaagtgaca
     1141 cgcttgctaa cgatgcctgc tttttgtacg gctgccttca atgcctttcg cgcaacggag
     1201 tcatgcagat gatggcggca taatttgccg ttatacgggt ggttgcagag cgtgctggag
     1261 ggaaagacaa acatccacgc cgcttgtcga taagcagaag ggtatttgtg atctaaagca
     1321 aaaggcagcg atggccctac gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc
     1381 tcaatgagtt attttattgc tgggattagg cgcgtgggca gtaggctgtt tctgcttttc
     1441 ccacccttac cgtcatgcac agtgatgcag ccattatcaa aatcaaaatc tttaacccgc
     1501 aaacgcaagc attcattaat gcgcaaacct gcaccataca gcagcgtaaa aataacttgg
     1561 ttgcgagtat ccataacctg caaaatgcgt tgcacttcat ttgcagagat aacagagggt
     1621 agccgtctag gcttgcttgc agggatataa tcaatatcgc ccaacggctg ttgtaaaaac
     1681 ctgttgtaca aaaaagctag ggcatttaaa gcgattttct gcgtgtttat ggctacatgt
     1741 ctgctgtttg ctaagctgga taaaaacagc ctgacctctt cactgcccat ggtctgagga
     1801 tgacgttttt tgtgaaacag aataaaacgc ttaatccagt gcaggtaagt tttttcagtt
     1861 ttcagcgcat aacctttttg tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta
     1921 gacataaaac gctccttgtc ttgcaactgt ctgcctatac agcctattct agctgggatt
     1981 taaaaaagtg cctgtttttt acgcctagag atgcttgttt accggtagag ttttaattta
     2041 atgctaaata aattaaaatg ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc
     2101 aggatagacg gcatgcacga tttgtaataa cagagtgtct tgtattttta aagaaagtct
     2161 atttaataca agtgattata ttaattaacg gtaagcatca gcgggtgaca aaacgagcat
     2221 gcttactaat aaaatgttaa cctctgagga agaattgtga aactatcact aatggtagct
     2281 atatcgaaga atggagttat cgggaatggc cctgatattc catggagtgc caaaggtgaa
     2341 cagctcctgt ttaaagctat tacctataac caatggctgt tggttggacg caagactttt
     2401 gaatcaatgg gagcattacc caaccgaaag tatgcggtcg taacacgttc aagttttaca
     2461 tctgacaatg agaacgtatt gatctttcca tcaattaaag atgctttaac caacctaaag
     2521 aaaataacgg atcatgtcat tgtttcaggt ggtggggaga tatacaaaag cctgatcgat
     2581 caagtagata cactacatat atctacaata gacatcgagc cggaaggtga tgtttacttt
     2641 cctgaaatcc ccagcaattt taggccagtt tttacccaag acttcgcctc taacataaat
     2701 tatagttacc aaatctggca aaagggttaa caagtggcag caacggattc gcaaacctgt
     2761 cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg
     2821 tcatatgaag atttcggtga tccctgagca ggtggcggaa acattggatg ctgagaacca
     2881 tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg aactatctac
     2941 cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg atgaagactc
     3001 tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac tcaactcaac
     3061 atggaacgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg
     3121 agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac agctccttgg
     3181 catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg
     3241 ctttactctc ggcggcattg acctgttcac gtataaaact agacctcaag tctcgaacga
     3301 aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac aattcattca
     3361 agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga gggaagcggt
     3421 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     3481 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     3541 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3601 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3661 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3721 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3781 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     3841 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     3901 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     3961 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4021 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4081 tgaagctagg caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt
     4141 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     4201 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagagagct
     4261 ggggaagact atgcgcgatc tgttgaaggt ggttctaagc ctcgtacttg cgatggcatc
     4321 ggggcaggca cttgctgacc tgccaattgt tttagtggat gaagctcgtc ttccctatga
     4381 ctactcccca tccaactacg acatttctcc aagcaactac gacaactcca taagcaatta
     4441 cgacaatagt ccatcaaatt acgacaactc tgagagcaac tacgataata gttcatccaa
     4501 ttacgacaat agtcgcaacg gaaatcgtag gcttatatat agcgcaaatg ggtctcgcac
     4561 tttcgccggc tactacgtca ttgccaacaa tgggacaacg aacttctttt ccacatctgg
     4621 caaaaggatg ttctacaccc caaaaggggg gcgcggcgtc tatggcggca aagatgggag
     4681 cttctgcggg gcattggtcg tcataaatgg ccaattttcg cttgccctga cagataacgg
     4741 cctgaagatc atgtatctaa gcaactagcc tgctctctaa taaaatgtta ggcctcaaca
     4801 tctagtcgca agctgagggg aaccactagt gtcatacgaa cctccaagag acggttacac
     4861 aaacgggtac attgttgatg tcatgtatga caatcgccca agtaagtatc cagctgtgtt
     4921 cagaacgtac gtccgaattc cagactatgt cgaactagat aacgcggagg agctggccga
     4981 cgcaatcatt cgagaggttg agttaaagga ggcatggctc acaagggtct tggggaactc
     5041 agccgatcct gacgcttctg tgggcaagtg ggaccggcgc ctagcgcagt gcggtggtat
     5101 tgatgagatg tcaagtgggg tgcaggcaac atacttcagt ccaaacggtc agctggtcgg
     5161 cttagtggca tacattgatg ccgaatggat ggatgaatag ctctgacgtt acgattcagg
     5221 tgagacaacc agatgggcat tgcgtacaag ctcagttgcc accgatgtgg ctacgagtct
     5281 gatcttcttt acctaggcca aggtatggcg atgcttcctg agcaaatagt cggaacgtgc
     5341 acatgctgcg acaatctaac gacgatttct ataaatgaaa gctacggagt atgctcactt
     5401 tgtggggcta aggatcgtgt gatatttaca agctatcagc atgaggcaag gccaagaaat
     5461 ccctgctatc tagagagaag tttcaaatat caatgcccaa gatgccatgc cttcagtatg
     5521 gagcctccgg atctaccgga gatgctttgg gactaatgct ccgctttgcc tcgggtatcc
     5581 gttcgaccta tgtttaagag gaaacgcacg tgtctgccgc caaagctctt gcctatgcac
     5641 tttgtgacaa gttggaaagc aaaaaagttg atgaagtcgt acgaaatctt gtttctagcg
     5701 gctggaatat tagggaagtg gcctgggaag cttcgtcctt tgatcatgaa atgccctact
     5761 ttgacacggg gcgggacttc gatattggat ttcctgacac gctgtcatcc aagattgagt
     5821 ttttgaccgc gaatggatca atcgtccaag cacgtgctcg cctgcacttc aagcaggctc
     5881 taatttttgc caaggcgtcc aaacatgtcg ccgcgctcaa gaactcattg gattactact
     5941 atggatcgtc tgtaatcccc cctcaaaacc acggaggcta tttgtagaaa attccattaa
     6001 tatgaactca aggtgttccc tacggacgaa tcagcgatga aggtggtgta cctggctatc
     6061 caggcggcga ccaagaaatg gacgatgccg atccgcaact ggaagccggc catgaaccgt
     6121 tttatgatcg aatttggtga ccgtttaaat ggccaccttt aactgaccgg caaaagcact
     6181 tacacaaaag ggtttacagg ctctttcagt cattccttta atgcgtaaat tgctctctaa
     6241 ccactctctc agcccaccct tccatatcct ctctcgatac ttgcagctcc cccaacccct
     6301 ttggatgatt cacccctcta tgcccgtctt gtccatataa ttgatcaaac aaactatttg
     6361 gccaattcaa tgagctcttc accactccac gccgtatctt ttcaaaatca ttccgccatg
     6421 tttcgctatc aacgccactc aacactttgg tagaaagcat tttcacccca tccgatgcat
     6481 ccacctctag caatacaaat tcactgccat tacgttgcac cccaacggct ttaatcactc
     6541 ttggtgagcc atctttacac agatgtaatc ggctacgccc aacttttggc aaaaccaagg
     6601 tttcttcaaa aagcacccga catgcaaatt tagtttttag aattgaaagt agctcatcaa
     6661 aagcagcaaa tcgattagca aaaatagagt tgtaattggt tgcatcctgc ttcccaccca
     6721 catctgccgc tgctaggaca ccacctaagt gtggctcatc agtactaacc aaattactat
     6781 tttcacttgt tgttgcttct tctttttcta aaatagtttg gctacttttt cttgccctgc
     6841 gagatttttc aattcgacta ggtctactaa aacttatcca tgtcggctca gcctctatga
     6901 ctcgtgtgtc tgtgtctata ttggctgttt cctcatcgtc caactgtaga tgctcatcat
     6961 caacaactga ctctgttgaa accgctatgt gctgcgtact accatcacct gcctcctttt
     7021 cctgaaaaga ggcatggcta atagctgttg tgctaggaag catagcgttg atttctaaac
     7081 ctactatttc ctcaactaaa taatccttat cctcgttaga agaacgtcct tttacatgta
     7141 atttccaacc ctgcatgggc ggagggtcaa agctaaagca ccagctttca acgccgttct
     7201 taatttctct attttgttga tagtgcctaa aaatactttc atacgaatcc ataacatctt
     7261 gatccgaaaa caaccaaacc aaaagctgca ctacggccga ctgctctaac gcccctttag
     7321 gaaacgatga actgggtaag atccttatct ctaaatgatc tcgctcaacc tcatactgaa
     7381 cgtcgaactc ttgctgtaaa gcggtactgc tcaaacagct tcgacagaaa taggagttaa
     7441 ttaaaaataa tgaccgagct agctctaact gtgggatgtg ataacgagtc ttgctatatg
     7501 gtgtttcagc ttcgtaaata aatgaactct gcgctccatc cttgttgcgt acggaagcaa
     7561 aggacgagag attaggaaaa tcgattattt tggctcgttg aagatccgat gcttcaaact
     7621 caatcacacg atccgcctta tttatttgct ttgttgtact gttaaccacc ttagctcgac
     7681 ttaataaagg caaatgagta aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa
     7741 caaaaatacg ccactcctta tgacccgagt tacggaataa atgaccaata tgtacaacct
     7801 gcacattgtc attaaatgta gctagcctaa ccactcactc ttccccataa accatttcca
     7861 agaatctttg tgcttcctca gttatccgct ctttagataa cgttgcactt cttaataatc
     7921 gccagcgcct aagctcaaca tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc
     7981 taatttgata atcttccaca ctctctgagt aacgctttaa gcaaagcgct accaaaggca
     8041 gtttctgtag attttttgca agagaggttc cgttaggagt ttgctttaac agccagctcg
     8101 atgtcgctct tgggtgatca aggctactat ctagacgctt tatgattctt aatagttgtc
     8161 gtacagcaat tcgatctctt tggttccaat caactctagg ggcgggtgcc agacgctctt
     8221 gttgatgctg ttgattccag tgaactagcc aatccctgtc atgtcggtaa agccaagcgt
     8281 atagcacccc accctctaaa gactgtcttg ccgccttaat tccttggtat ttatgcacta
     8341 gttgctgcca gtctttatgc ttaacagata aatcttcaga gttaggttgc acagactggc
     8401 taacaggtct tgtcgttata gagtgctcag taagagcact tgcctgctgt agcgcttcta
     8461 taaccgttag ttttggcaat aaggcttgcc acacaatact atgctgtaaa taactaaagg
     8521 cttttctatg cttacggaat atacttttca gccaacacgt gtccttgttc tctgccaact
     8581 ttaaatccag tttctctagt gcctcatcac taaaagtctg cctcactctc tccgccacca
     8641 agtcatgacg aatgtgcttg cttttggtta gccctagatc ctgcgctaag cgctgataaa
     8701 acagcgtcca ctgctcaagg cttggggaaa gctcttgcgc tcgtggagca tctaacagag
     8761 gggctatata agcagctagt gctgttaatt gagataggga gtctttgggg tagtctgaaa
     8821 gcagctcagt atgacccaaa gcccaaaatt gatgtcggtg atcatctaca gctctatcaa
     8881 agaagactaa agcaccgtgt tttggacaat atggcaaagc gggcaaatac caatctcgtt
     8941 gccaaaaggc ttccccatac ctatttagct gaagagcaac gcaatcaggg cagtatctaa
     9001 agcggttatc gctcttaact ctagaagcag cgactcctag cattaaatgc accgcacctt
     9061 gcgcttggta ctccattaac cgaatagctt cgtctcggcg ctccttgcct acaaacggag
     9121 catataaagg gaataaggta tgctcataaa taagctgctg aacagcgtaa cgtcctgttt
     9181 gatgtagatg tcttgctatc acacctaaat gcgagggcag acctaaggta gcgaccacct
     9241 tgcggttgcc atacacctca tccaacagct gcttaggact aacaatccct tgataaacgc
     9301 ctgcccgtgc aatagtgcta taaatcagct cattcgagta cggaacagga aagtttctca
     9361 tggctaacct gcttgtttaa ataagctcgc catatccact attacccctt tcccttttaa
     9421 ccgttcatgc atggtttttt caggttggcg ttgtgaatag atataacgta aatccgtatc
     9481 aggcaagcta tcccagtcgc tgggcttgac tacctttata gccgaaacct ttttactctt
     9541 ggagggcttt tctagttctg acactaccgt ttcgccttcc atcaaccact gcaaaacaag
     9601 aggcagtaac ttttgtcttg tcatcgttgg attctggcta aacgcttttt taatagtggg
     9661 aattaacagg cttgaatcgt aatcctcttt cagcatcaga tataaatgac gctgatcttc
     9721 ggtatctaac tcttgaaggg ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc
     9781 tagctgaagt tggattaacc gtttatcaat ctcgggaacg actagatcag aataacgagc
     9841 aatgcgttct gggatacccg agcgtaatgc ctctagcatg gggtgcacag gctttaactc
     9901 atcttgatac acttgccgca ataaaccagc ggtaatacgc tcattgccta aagctagcgc
     9961 acggagctga gcgagtacaa aaagttttac tacaatgtcc atcactcctt ggcttagctc
    10021 ataccacaca tcacggacct catccgataa cagcgcatct ttgcgttgta aaagctgtaa
    10081 ttgccaaaga ttatccgtaa aagcgatcca ctcttgattg ggctttccac gttgcgtttg
    10141 ttgtatagga tcccagaata tagctccaaa ccctgcccct ctacgtgcag accgcaaatc
    10201 agcctcaaaa atctctcgtg ctttaggggt accaatcaac atcactggta cgccaataat
    10261 attcaccatc gtcacaaaaa agttcagcat ctcttgagat ccacccgaac gagagcggct
    10321 taaatgctga atttcatcaa taaccaacaa ccctaaagca tgtgcattgg ctatttgcga
    10381 catcaaagcc aacatggttt ctataccatg acgttttaag ccataacgac gctcatagtt
    10441 cgagcccaag gctcgatcca acgctctgaa aaaattcaag cagatttctt ttagcgaacc
    10501 attatgcgag cagtctattt tcaaatacac cacctgctct acattgagtt cacgatggta
    10561 aatcacctga ggatacgtgg ctagaatacg atgaagagag gtcgtcttcc cactaccaga
    10621 acaaccaatt aacaataagc tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc
    10681 caactctccc gtttgaacac gctcataacc attttgtaaa tgcttttgta aatctcctgt
    10741 tttaggattt ctgcctacgt agccacctcg aatcatgacc gaaatacgct cacttagtag
    10801 caaatgcgta cctaatggct gaaaatagtc atctggaata cgacaaatgg tatgagctct
    10861 gataacacgg gacttttgca agtcagagga agtaagctgt aaagaggatt tcagtgatgc
    10921 agcactattc actgactctt gtaatggtgg taaggcctcg ataaaaggat tatcacgata
    10981 agcctctacc cccgtatcac gataaactgc ttgaatccgg gtagcactca tgactcatcc
    11041 ttttctggtg gatcctgaaa taattcaggc acgtatgtgg gtaggctgta atcttcttga
    11101 tcatccgctt ccactgcgtt gaaaggaata actttagcct catcacctga agagcttggc
    11161 ttcaaatgct ccgcacgttt tttacgctcc gaggtcacgg cttctttttt attagtttta
    11221 atctgcttaa tgcgtgttga tttgggctca gtagtactgg gcgttaactt attcgctttc
    11281 tgaatggttt gctgaataaa cgcctcaagc tccctgcgtt tagttaactc atcctgcttc
    11341 gcattggctt tattgtgttt ttcttgtgct tgtatatccc aaacctccca aaatgagaga
    11401 cctttaaact gccgactacg ttccgtcaga ttacagcgcc aaaatacacg gctgccaact
    11461 tgcggaaaca aataaatcgt atcaaccagc actgggtcat aagccgcttc taaatgttga
    11521 ggtctagcta tatcagtgct ccgctgcaac caaccctcac gcagaatctc tgaccccgag
    11581 taatacaaac cccacaaatt aacgccaaat gaagaaatag agacctttcg gcgaggcagt
    11641 aacgctactc gcaactgctc ttgctccaca gcccttaaac tacctgtacg atgctgcata
    11701 ccccattgcc atagctggac aggaatagac ggtaaatctg taggaaaatc agcatctcga
    11761 tcgtatttat ccatcaccag atggttattt ctgaataaga tcgtacgcaa aataatttgt
    11821 gtgaactcaa ataccgacag agatgcatct aacctatagt ctgtttcacc atggcttttg
    11881 atccgactgc cctctacaat gccaggtgca aaggacttaa actcggcttg tagtgttcta
    11941 aaagtgcttt ccactatgcc tttagcatcg ccacgtctag gtggagcact ttccactcgc
    12001 acattaaaac tagaaactaa ggcttcgacc tgatgactca ttaattcgcc acggtccgct
    12061 agcaacacat ctggcaaacc tacacacggc cagtctgagc tactaatctc aatatcatgc
    12121 tgggcacaaa tggccgtttt gtcagagcaa gcatttacaa aagcctgcat cgccaccaca
    12181 taagacggat tttcaaagcc gatataaaag cccgtgatca tccgactaaa cacatcaatc
    12241 acaatgtaaa gcgttggtct tcctatgatt ttttggcgat catgatgatc cactaaataa
    12301 atatcagcaa tcgtggcatc aatctcataa cgactcccag ggcctaacgc ctgagaagtg
    12361 gctgtactac ttaagggtcg tacgtctttt ttatataccc ctgctttaac tctagacttt
    12421 aagcgctgag ctttagggta ttctcgatca taaaaataac gaaactgacg tagtgttggg
    12481 taatcctctt ggggaatgcg aggaaaatac tgagcaaaca agtccacaaa tcgtctatag
    12541 gcaacggtgg tctttgtacc tttttgattt aacaggtgct tttctatggt caacctaaaa
    12601 aggcgttcaa tctcgggcgt taccttggtt ccttcaccct taccatattc tctggctcgg
    12661 ccaatctttg ctgttcctgt cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag
    12721 tcaggaatta atgcattagg cgtttgacca cgctgccagt aacggcgtaa caacttataa
    12781 actgtagcct tagtaacctt atgttcttgg accacatgct caacgagttc gcttctgact
    12841 ttagggtcga aacgatcctt actattaata ataggaagaa tttttcgata atcctcctcg
    12901 cgcttctgga agctgacaga atctacagac ggctcttcta aatcaagatg tacataaggg
    12961 tcatcagttc taactaagcg gccttcatct aaatactgca ttagcaacaa ctcagcccta
    13021 gcttgtggaa ctcctttatc agcgcttatt tgcatccaga caacttggcc atcctctata
    13081 gccaaaatgc gatacggatc attatcaaat agcacaacct cattaatttg ccacatagcg
    13141 caactcctcc atattcacta cttggctaat acagagatct gcacacttat ttgccctgaa
    13201 agacttataa atattgaact taataaaacc atttgcggtt aaggctcgta tctcactcaa
    13261 tgttttgcct aactccaaat cataagcaat atcaacctgc ttacagacat tgataatgtt
    13321 ttcatctcct ttttcttgca ggatatgggc caatggggat agttgtgcta aaagctccgc
    13381 agaaacttct tctgttttca ctgaataaag ccattcaata ttttctttta ctacgggatt
    13441 tatttcttta tcagtaaaaa tgaaccaagg aatttgcttt tgctgccaat agcgacgctc
    13501 tagttctagt ttttctaagg tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat
    13561 agcaaactgc tcaaaaggac catctttgca gtccactaaa aaatcagtag acataacctg
    13621 atctacacca cgaataacag gatgcttaat accactatct attgcaatct gcctggtatc
    13681 actaggtaat aaggggaact gctcgcgtat atctagcacg ctgctctccc actcaagact
    13741 gagaaaaaca gcaagctcta agtcagatag caaatgatgg actcgtcccg tcttatgaga
    13801 ataaatacgg tgggaacgac ctgaagaagg aacttcttgt actgttagcc atggaatata
    13861 gtctttacca tgcccttggc cacgcccctc tttaatacga cgggcaattt gcacttcaga
    13921 aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg
    13981 gactgatttc agtttacaac tattctgtct agtttaagac tttattgtca tagtttagat
    14041 ctattttgtt cagtttaaga ctttattgtc cgcccacagc ccttttttat ctatgatagt
    14101 gattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg
    14161 atcagcgcga catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc
    14221 acctcttcca catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca
    14281 tcctgatcgg cgaagacata caattggccg ccacgcgcgc gaacttcttc aatgttggat
    14341 ttcagttttt ccagcaattc gttgttcggt gcaacgacga taaccggcat atcggcatca
    14401 ataagcgcca gcggaccgtg tttcagttca ccagcagcgt aggcttcagc gtgaatgtag
    14461 gagatctctt tcagcttcaa tgcgccttcc agtgcgattg ggtactgatc gccacggccc
    14521 aggaacagtg cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc
    14581 tgagatagca tctgctcaat acggctcggc agcgcctgca gaccatgcac aatgtcatgt
    14641 tcaatggagg catccagacc tttcaggcga gacagcttcg ccaccagcat caacagcaca
    14701 gttaactgag tggtgaatgc tttagtggac gccacgccga tttccgtacc cgcgttggtc
    14761 atcagcgcca gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc
    14821 gaaccaaggt aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg
    14881 ccagactgtg acaaggtgat catcaggctg ttacgacgca cggcagattt acgatagcgg
    14941 aattcagagg cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg
    15001 gaaaccatac cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc
    15061 gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggctgatg
    15121 cgcccggtaa gggtgttttt tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag
    15181 tgacggtaaa tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt
    15241 tttacttccg cgccagtttt atcgaagatg tttaccgaac ggcgagtgat ttccgcaata
    15301 tcgccctctt caaggaagat aaagcgacgg gtcaccggca acagcgccag ctggtcagaa
    15361 gcgataaagt tttcgcccat acccaggcca atcaccagcg gactaccaga acgtgccgcc
    15421 agcagggtat ccgggtgacg ggagtccatg atcactgtac cgtacgcacc gcgcagctgc
    15481 gggatagcac gcagaacagc ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc
    15541 agatgggcaa tcacttcggt gtcggtttca gaaacgaagg tatagccacg cgcttttaac
    15601 tcttcacgca gcggttcatg gttttcgatg atgccgttat gcaccaccac aatgtgttca
    15661 gaaacatgcg gatgcgcgtt agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga
    15721 gcaataccgg tgccgccatc cagaggatgt tcttccgctg cctgagccag catctggact
    15781 ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccggca
    15841 gagtcatatc cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca
    15901 cgttgcgcga tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt
    15961 cggtcaacct gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt
    16021 tttatagtta ctgcttgtgg gagggagatt atgttatctc ctcatcccag atgaccggat
    16081 tacccggcca gaattacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg
    16141 gcacacggct gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag
    16201 caatggtcgc gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa
    16261 acacatcgtc gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg
    16321 ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgcc
    16381 cgactttcga gcctttaccc agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg
    16441 caccttccag caactcagca ccaggacgca aacgagcaaa cgggccaata gtacaggccg
    16501 ctgccagatt cgcgtcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca
    16561 cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc
    16621 cctcgatgat aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca
    16681 gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt
    16741 cggactggta aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt
    16801 cgcttaaacg ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa
    16861 taatgtcggt gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc
    16921 gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga atctgacgct
    16981 gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga
    17041 tacgtccata accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg
    17101 gtttagcatc acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga
    17161 gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg
    17221 tacccagctg ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa
    17281 gcagatcgcc gccgtgaccg tacaccaagt gaacgtgcgc tgcgcctaat tcattcgcag
    17341 catcaatgac atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat
    17401 cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca
    17461 acatacgcgt cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact
    17521 acattttttt catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc
    17581 tgacgaaagt gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt
    17641 accttcattt aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg
    17701 aaaacagggg tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc
    17761 gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc ttttcaagcc ggtgttacat
    17821 cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga
    17881 cgcctgagcg taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt
    17941 cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac
    18001 ggtcacgttg ccaggctgca cttcaagaat gccgccagac agatagataa actcttcgtg
    18061 accgtgctgt ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg
    18121 gccagggtag atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc
    18181 agagaacatt tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc
    18241 ctccgattaa ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc
    18301 atgtagaacg cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca
    18361 cggatggtgt ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg
    18421 aagaacggct gggacaggaa gcgctggatc ttacgagcac gcgctaccac cagtttgtct
    18481 tcttcagaca gttcatccat acccaagatg gcgatgatgt ctttcagttc ctgataacgt
    18541 tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg
    18601 tccagctgac ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg
    18661 atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg
    18721 tcagtcaagt catccgcagg tacgtatacc gcctgtacgg aggtgataga accagttttg
    18781 gtggaggtga tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct
    18841 accgctgaag gcatacggcc cagcagtgcg gatacttccg taccggccag ggtgtaacga
    18901 tagatgttgt caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg
    18961 gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac
    19021 accagggata ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc
    19081 tcacgagtac gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg
    19141 ttacgaatga gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca
    19201 actttaccgc ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc
    19261 agttcctgag agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc
    19321 tcttcttcac cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg
    19381 cccagagtcg ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga
    19441 ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc
    19501 tgctgctgaa cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac
    19561 acgcgcggta cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca
    19621 atctttccag tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc
    19681 ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca
    19741 actgcagctc tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac
    19801 gggcggcctg ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat
    19861 aacgacgcag cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg
    19921 atttatgttt cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg
    19981 gaacctgaga catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc
    20041 cttcgtcgta agcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt
    20101 tatcccccat gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca
    20161 cgcctttcga gccgatcatt gcgaggtcgc attgaacgct tttgtcggtc caggtcttca
    20221 tttccgccag tagttttttg aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg
    20281 tcgacaccac caggtagccc acgcgtttaa cgtcgcggtc ttccaggtaa gggtgcttat
    20341 attccagatt accgtgtgca aggtgaccaa tcactttgcg catggtttct gcataaggac
    20401 ggctggccgc catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg
    20461 ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc
    20521 cggccatgag cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta
    20581 ccaggattgg gttgctttga aggaatcgag gatgcctttc agcttgcctt cgatttcgtc
    20641 gttgtagcca ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta
    20701 agccagcaga gcggcttcga agctgccaat tttcgacagt tcaacatccg ccaggtaacc
    20761 acgttctgct gcgaacagaa ccagagactg ctgcgcaacg gacatcggcg catactgttt
    20821 ctgtttcagc agttcggtca ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc
    20881 aaggtcggat gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg
    20941 gataccaccg gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac
    21001 ggaaataccc gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa
    21061 gatctgacca tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc
    21121 ctgagtttcg ataatcggca gtgcggtcag agaaccggtt ttccctttca cttcaccttt
    21181 ggtgaaggct tcaacgtatt cggcgttaac acgtgcagca cgctccagca gacgagagtg
    21241 gaggtagaaa acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga
    21301 gatctgacgg taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc
    21361 accgcggtca cggaagtatt cgcccattgc gcaaccggca tacggtgcca ggtattgcag
    21421 tgcagcggat tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc
    21481 ttccagttta cgtaccacgt tagaaatggt ggacgctttc tggccgatag cgacatagat
    21541 acatttgata ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc
    21601 tgtctgacgg tcaccgatga tcaattcacg ctgaccacga ccgattggga tcatggagtc
    21661 aacggcttta taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg
    21721 agcgattgct tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg
    21781 tgcacccaga gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat
    21841 acggccagta cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac
    21901 cgcacctaca gagtcgcgct caaggttcag tgcgatagcg taacggttac ccggcaggga
    21961 gatcatttca ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac
    22021 agaaacaata gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg
    22081 cttgatcagt tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg
    22141 caagacgtct gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc
    22201 cgctcggatg ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt
    22261 gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc
    22321 ggcagcggaa atgacgtcta cctcagcggt agcctcactc acggcacgaa ggtgaataaa
    22381 ctgctccaga acatccggga gcgcgttaag acgaccattt tcagccatta cccgaatcag
    22441 gttctgaccg ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcgagcgt
    22501 ttctggcgca agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc
    22561 ggcaaacgcc agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa
    22621 agctgctttg gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct
    22681 tacagttcag cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt
    22741 tcgatgatct tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca
    22801 cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct gggccacgat tttagtacgt
    22861 tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc
    22921 tcgatgatta cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt
    22981 gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt
    23041 tgacgttttt cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg
    23101 aacaggacaa acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc
    23161 ctctatttag ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac
    23221 agcgaacatc acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc
    23281 cataacgata aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc
    23341 ttccaggaat ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc
    23401 catcatcaca gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc
    23461 cagtttgttt cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg
    23521 acagatagac gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt
    23581 ggaaaatggc ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa
    23641 tcagaatgaa aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa
    23701 ctggtttgga cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt
    23761 gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc
    23821 tgtagaacag aatcaggata aatacgccca gtgccataga cagcgttacg ttcacgtccg
    23881 cagacggaac cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca
    23941 ggtcgatagg cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg
    24001 ccagcggagc aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa
    24061 agccgatcac cagctcaatc gcggtctgaa acttacctgg cacaccgctg gtcgcctttt
    24121 tggctacgct acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg
    24181 agtcaatatt gattgtccag aaggtggctg gggggttttg tggatccacc agcgagaatg
    24241 tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat
    24301 tttctgaagc catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg
    24361 aaccaccagc accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc
    24421 caacgccacc accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa
    24481 tgtccaggcc acccggcctt tcgctggtgt atacgcctgg tgacgccagg caaatatcat
    24541 aaacaaaacg ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa
    24601 ggggtctttg aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag
    24661 aagcttccga gcaacgtttc gactcacgag cgacacagac atcacgtttt tcactcctgc
    24721 tcccttcgag gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc
    24781 aaaaagcggt caaattatac ggtgcgcccc cgtgatttca aacaataagt agccaaaagg
    24841 tgaataaatg tttaaatatt tttccagtgc atacaattgc gacttttccg ctaaccctgt
    24901 tcgatcatga aaaactgtaa ataacgcgta aacactagtg ataaagcgtg cttcagatca
    24961 catattgcgc atgttcgcgc acagtatatt tatttacttg gcaaatgatg cctttgcaag
    25021 tttatgatat ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa
    25081 aaccacccat tgacattttt aataatgttt taacagccaa tgatggttct tagcgccgat
    25141 ttttagcaga ctgatatttt cactaatgac ttattttctg cttaccaaaa aaagccacgt
    25201 tatcttgttg atacaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa
    25261 aacttggttg ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt
    25321 aaattttatt tgctttaatc accaccagat gacgttcgcc atccagagct ggaacctgaa
    25381 gtttaaccac tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca
    25441 tttgcccttt cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca
    25501 ccatatcgtt cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag
    25561 gaaactcttc taccctgctc tgtactggct caatattctc cagtttaagc tcatgttgca
    25621 cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag
    25681 gacgcacgat agagagtgga atgcctggca gtcctggtcc ggtgccgaca tcgataaacc
    25741 gttcaccttg cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct
    25801 cattaggatc gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca
    25861 cgtaggcaat aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca
    25921 gtaaggagag tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg
    25981 gtgcttacca ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca
    26041 gccacaccag cagaatggag atagccgcag gcgtgacgcc agaaatacgc gaagcctggc
    26101 cgatagaggc cggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata
    26161 cctggcggta atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga
    26221 tctcatcttg ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg
    26281 ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat ttttcataag
    26341 tcatttccgg acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca
    26401 ggtgagcatt cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac
    26461 gctgacgctc acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat
    26521 ccaccaggcc cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgca
    26581 gcatcagacg atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag
    26641 tgcacaggtc atcaaccagt acgccgagat acgcctgaga acgcgccgga gcccaacctt
    26701 ctttgtcagc agacagacgg gcagcgttaa gaccgaccag caaaccttgc gcagcggctt
    26761 cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc
    26821 tctccagcgt cggtttcagg tcgcgtggat cgaagaagtc atactcaatg gcataacccg
    26881 gacgcacgat cttcgcgttt tccatcccct gcatggagcg gacgatttgc atctgcacat
    26941 cgaacggcag gctggtggag ataccgttcg gataaatttc gttagaggtc agcccttccg
    27001 gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg
    27061 atgggcagta gcgtgggccg acaccttcga tcacccctgc gtacattggg ctacgatcga
    27121 ggttactgcg gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct
    27181 gctggggatg ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat
    27241 gctgttgcgc cagcacacta aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg
    27301 ttttcagacg accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg
    27361 gcggatcacc agcacgacca ccgctgtaat tatccagacc gatatgaatt ttaccgtcga
    27421 ggaacgtccc aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa
    27481 cagcaccgac cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca
    27541 tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg
    27601 cctgagctcg ggtagcgcga accgccggtc ctttgcttgc gtttagtatc ctaaactgga
    27661 tacccgcttg atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca
    27721 gatgtccctt cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga
    27781 tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg
    27841 tgcctgcatg acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga
    27901 ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac
    27961 tttgtcggct tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag
    28021 agatctgttc tattgtgatc tcttattagg atcgcactac cctgtggata acaaggatcc
    28081 ggcttttaag atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga
    28141 ccgtataagc tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg
    28201 ttccttggat aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc
    28261 acgatctgta tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat
    28321 cttccggaat gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac
    28381 cggaattttt gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac
    28441 gactaccaat accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca
    28501 atgcttcata gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga
    28561 taatcagcca gatccccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg
    28621 tggtaaaacc cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc
    28681 cgagggtgct gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt
    28741 ggcgtattgt acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa
    28801 agccgattat ggcttgatgg tacgcatgat cgggttttgc aggacgatca atgtctcggt
    28861 ggactgaatt tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat
    28921 cgaacggcac attactttta taaagatgct gtagtggcct gttgtgtaat aggcttcagt
    28981 gacttcagca aggctttcca gctttgccag cgcggaaggg tagtctttgg cgctctttaa
    29041 tataatgccg ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg
    29101 cgccccggta atgatccccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg
    29161 actgacgcca aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc
    29221 ttccaggatg ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc
    29281 agattgatga ttcattctat tttagccttc ttttttaatg aatcaaaagt gtgttaggct
    29341 ttttattgaa tgattattgc atgtttgtcg gtttttgttg cttaatcata agcaacagga
    29401 cgcaggagta taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt
    29461 gaaatctcac ttttctcgcc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc
    29521 gattcttagc cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt
    29581 gcaggtaaaa gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa
    29641 gtggaaacgt cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca
    29701 catgaaagcc cttcgccccg atgaagaccg tctttctccg ttgcactcgg tctatgttga
    29761 ccagtgggac tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag
    29821 cacggtagag gcgatctggg cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt
    29881 tggcctggca ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc
    29941 tcgttatccg gatctcgatg ccaaagggcg tgagcgggcg atagcgaaag atcttggcgc
    30001 ggtattcctt gtcgggattg gcggcaagct gagcgatggt catcgccacg acgtgcgcgc
    30061 accggattat gatgactgga gcaccccgtc agagctgggt catgcgggtc tgaacggcga
    30121 tattctggtg tggaaccctg tactggaaga tgcgtttgag ctttcttcca tggggatccg
    30181 cgtggatgcc gacacgttga agcatcagtt ggcgctgacc ggtgatgaag atcgtctgca
    30241 gctggaatgg catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat
    30301 cggccagtct cgtttgacca tgctgctgct gcaactgccg catatcggcc aggttcagtg
    30361 tggtgtatgg ccagctgctg ttcgcgagaa cgtcccttct ctgctgtaat aatttatcgc
    30421 cgccagcgtc tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa
    30481 atgcgcatga tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga
    30541 tgtacccgct gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgagcgata
    30601 aaatcagaaa tcaccaccgc atcggcatca aaccattccc tgctttgcaa gcgttccata
    30661 atggcgcgaa aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg
    30721 attgcttgtt cgatgccttg ggggcctgaa agctcataac ggacgatctc ggtggaaaat
    30781 agcataatat agcagcgccg gttttcagcg agagcaatgc gcatcaaggc caggcagaac
    30841 gctttcgcac actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata
    30901 aacggcccgc gcggctgttc atcgtaatct ttatgtacca ccggacgttc gatcactttt
    30961 tcacgccacg actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg
    31021 taaaactcat actccagttc cgttatccct agtgtcgcca gttctggcgg cagaagacgt
    31081 aaaatatcat cgctttgttg cagaccatca acctgctcag gaaccgtcgc cggttcgcgc
    31141 accatggtgc ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccga
    31201 gaacgcccca gctgctctgc caggcgtttc agttccggct gttcgttaag aaattcaccg
    31261 tatttcacaa tcaactgata gtcgccacgt ttaagctgac cggcgctcat atcccacaga
    31321 cgaccagctg cagtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg
    31381 cgttcttgaa cttcactcaa cagttgttcg cgttcttctt ctaatagctg ttgatttaac
    31441 gtcgttgctt gcacgatcag acttaaacgc caacgctgga gaaaaagcgt gtgtaacgct
    31501 gaagttattg tgctgttagc atcaaccaac tgccgggctt gttctgccca tggggaattc
    31561 agacgatgca gtaagtccag gatctgtggt agctgcacaa taaactgcgg cgtggagagg
    31621 agctggcttt gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct
    31681 ttcagccgac tgcgcagcgc ctcacgccag cggggaacat catcagtgat tgccgccttc
    31741 aggcgtggga atttttcaaa gaagaccgcc agctgcggtg aggccagcag tgcgatgatc
    31801 atctcttcga tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt
    31861 agcattgttg tgcctggtga atctgtgcac cgacatcctg taggctggct tcaatgcgac
    31921 ctaaccagtc gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct
    31981 tgcgccagtc gctttcaagc tcttccagtt gttgttttat ttcaccaggc agaccttcag
    32041 ccgatgaacc gggaagtgcc agcgtactgc cttgtaaact tacatcgcga acgacaagat
    32101 gttgggcgct atcaacttcc agattcagtt tctgggcaaa gccgataccg ttcagtttac
    32161 cgcgaatttc accacctttg ctcagccact gttccagcgc gctacgctca aaggagatat
    32221 gaaccacttc catatcatgc agttttaacg gtttttgcag cagtagagtc agagtggaag
    32281 cagtaacgtt aacaggaagt tgatactgct gacgacggct gaaaatgccg cccagacgaa
    32341 ttaccgttaa ggctgtttta tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa
    32401 tcgcgccaag gcgagtcaac atcccttgct gttgccaggc atgaccggtc atcaatacat
    32461 caatttgttg ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca
    32521 aaatgagatc taccggggca acagcactgc gaccgctaaa aaaggcgctg gcctgcaata
    32581 atcggatcgc ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca
    32641 gttgcaggcg cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa
    32701 tctctttctg ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat
    32761 tgtcgttttc atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt
    32821 tatctaacca cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt
    32881 ccggcagctc gttggaggcc gccaccagca gacgcatcgg gattttttct acgtgtgcac
    32941 cgttgcggaa ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg
    33001 ctttccagat ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac
    33061 gttcatagcg cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg
    33121 gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta
    33181 agcgccgggc gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac
    33241 cacttaatgc cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt
    33301 ccaacgaact gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga
    33361 agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt
    33421 ggctaaaata gtctcaaag
//
LOCUS       DAJVYM010000031        33147 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVYM010000031 DAJVYM010000000
VERSION     DAJVYM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.031"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="OQS60_001363"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="OQS60_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS2387158.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OQS60_001364"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OQS60_001364"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS2387159.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="OQS60_001365"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="OQS60_001365"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS2387160.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="OQS60_001366"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="OQS60_001366"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS2387161.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6628
                     /gene="hemX"
                     /locus_tag="OQS60_001367"
     CDS             5417..6628
                     /gene="hemX"
                     /locus_tag="OQS60_001367"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS2387162.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            6631..7827
                     /gene="hemY"
                     /locus_tag="OQS60_001368"
     CDS             6631..7827
                     /gene="hemY"
                     /locus_tag="OQS60_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS2387163.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8489..8650
                     /locus_tag="OQS60_001369"
     CDS             8489..8650
                     /locus_tag="OQS60_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387164.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(8734..9969)
                     /locus_tag="OQS60_001370"
     CDS             complement(8734..9969)
                     /locus_tag="OQS60_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS2387165.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10116..10192)
                     /locus_tag="OQS60_001371"
     tRNA            complement(10116..10192)
                     /locus_tag="OQS60_001371"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10156..10158),aa:Pro,seq:tgg)
     gene            complement(10235..10322)
                     /locus_tag="OQS60_001372"
     tRNA            complement(10235..10322)
                     /locus_tag="OQS60_001372"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10286..10288),aa:Leu,seq:cag)
     gene            complement(10343..10418)
                     /locus_tag="OQS60_001373"
     tRNA            complement(10343..10418)
                     /locus_tag="OQS60_001373"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10383..10385),aa:His,seq:gtg)
     gene            complement(10477..10553)
                     /locus_tag="OQS60_001374"
     tRNA            complement(10477..10553)
                     /locus_tag="OQS60_001374"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10517..10519),aa:Arg,seq:ccg)
     gene            complement(10654..12039)
                     /locus_tag="OQS60_001375"
     CDS             complement(10654..12039)
                     /locus_tag="OQS60_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2387166.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12230..12970)
                     /gene="rffM"
                     /locus_tag="OQS60_001376"
     CDS             complement(12230..12970)
                     /gene="rffM"
                     /locus_tag="OQS60_001376"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS2387167.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12973..14325)
                     /gene="wzyE"
                     /locus_tag="OQS60_001377"
     CDS             complement(12973..14325)
                     /gene="wzyE"
                     /locus_tag="OQS60_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS2387168.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14322..15401)
                     /gene="rffT"
                     /locus_tag="OQS60_001378"
                     /gene_synonym="wecF"
     CDS             complement(14322..15401)
                     /gene="rffT"
                     /locus_tag="OQS60_001378"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS2387169.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPEKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15398..16648)
                     /gene="wzxE"
                     /locus_tag="OQS60_001379"
     CDS             complement(15398..16648)
                     /gene="wzxE"
                     /locus_tag="OQS60_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS2387170.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16650..17780)
                     /gene="rffA"
                     /locus_tag="OQS60_001380"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16650..17780)
                     /gene="rffA"
                     /locus_tag="OQS60_001380"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS2387171.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17785..18459)
                     /gene="rffC"
                     /locus_tag="OQS60_001381"
                     /gene_synonym="wecD"
     CDS             complement(17785..18459)
                     /gene="rffC"
                     /locus_tag="OQS60_001381"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS2387172.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18437..19318)
                     /gene="rfbA"
                     /locus_tag="OQS60_001382"
     CDS             complement(18437..19318)
                     /gene="rfbA"
                     /locus_tag="OQS60_001382"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS2387173.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19337..20404)
                     /gene="rffG"
                     /locus_tag="OQS60_001383"
     CDS             complement(19337..20404)
                     /gene="rffG"
                     /locus_tag="OQS60_001383"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS2387174.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20404..21666)
                     /gene="wecC"
                     /locus_tag="OQS60_001384"
     CDS             complement(20404..21666)
                     /gene="wecC"
                     /locus_tag="OQS60_001384"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS2387175.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21663..22793)
                     /gene="wecB"
                     /locus_tag="OQS60_001385"
     CDS             complement(21663..22793)
                     /gene="wecB"
                     /locus_tag="OQS60_001385"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS2387176.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22849..23895)
                     /gene="wzzE"
                     /locus_tag="OQS60_001386"
     CDS             complement(22849..23895)
                     /gene="wzzE"
                     /locus_tag="OQS60_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS2387177.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23907..25010)
                     /gene="wecA"
                     /locus_tag="OQS60_001387"
     CDS             complement(23907..25010)
                     /gene="wecA"
                     /locus_tag="OQS60_001387"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS2387178.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25250..26509)
                     /gene="rho"
                     /locus_tag="OQS60_001388"
     CDS             complement(25250..26509)
                     /gene="rho"
                     /locus_tag="OQS60_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS2387179.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26594..26695)
                     /gene="rhoL"
                     /locus_tag="OQS60_001389"
     CDS             complement(26594..26695)
                     /gene="rhoL"
                     /locus_tag="OQS60_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS2387180.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26836..27165)
                     /gene="trxA"
                     /locus_tag="OQS60_001390"
     CDS             complement(26836..27165)
                     /gene="trxA"
                     /locus_tag="OQS60_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS2387181.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27296..28561
                     /gene="rhlB"
                     /locus_tag="OQS60_001391"
     CDS             27296..28561
                     /gene="rhlB"
                     /locus_tag="OQS60_001391"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS2387182.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28695..30179
                     /gene="gppA"
                     /locus_tag="OQS60_001392"
     CDS             28695..30179
                     /gene="gppA"
                     /locus_tag="OQS60_001392"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS2387183.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30226..32247)
                     /gene="rep"
                     /locus_tag="OQS60_001393"
     CDS             complement(30226..32247)
                     /gene="rep"
                     /locus_tag="OQS60_001393"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS2387184.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            32422..32622
                     /locus_tag="OQS60_001394"
     CDS             32422..32622
                     /locus_tag="OQS60_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387185.1"
                     /translation="MLLLFQSLLRRGVLVAINFSPKVGEILDCNFGNYPVSQNGQFST
                     TYYDGRIPPEMIKNRLVVRLAP"
     gene            32653..33147
                     /locus_tag="OQS60_001395"
                     /pseudo
     CDS             32653..33147
                     /locus_tag="OQS60_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8367 a         8939 c         8330 g         7511 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcaccgc aaggtgatac
     6601 tccggcagcc gcgccgcaag gagaataacc atgctaaaag tgttattact ctttgttttg
     6661 ctgattgcgg ggatagtggt tggcccgatg attgccggtc atcagggtta cgtgctgatc
     6721 cagactgaca actacaatat cgaaaccagc gtcacgggcc tggcgattat tttgattctg
     6781 gcgatggtag tgctgtttgc cattgagtgg ctactgcggc ggatcttccg cacaggcgca
     6841 cacacccgtg ggtggtttgt cggacgtaag cgtcgccgtg cccgtaagca gaccgaacag
     6901 gcgctgctga aactggcgga aggcgattat cagcaagttg aaaagctgat ggcgaaaaat
     6961 gccgatcacg cggaacaacc ggtggtgaac tatctactgg ctgccgaagc cgcgcaacaa
     7021 cgtggtgatg aagcacgcgc caaccaacat ctggaacgcg cagcagagct ggccggcaat
     7081 gacaccattc cggtagaaat cacccgtgta cgtctgcaac tggcccgtaa tgaaaaccat
     7141 gctgcacgcc acggcgtgga taagctgctg gaagttacgc cacgccatcc ggaagtatta
     7201 cgtctggcgg aacaggcgta tatccgcaca ggtgcatgga gttctctgct ggatatcatc
     7261 ccatcaatgg cgaaagccca tgttggtgat gaagaacatc gtgcaatgct ggaacaacag
     7321 gcatggattg gcctgatgga tcaggcgcgt gccgataacg gtagtgaagg tttgcgtaac
     7381 tggtggaaaa accaaagccg gaaaacgcgt catcaggtag cgttgcaggt ggcaatggcg
     7441 gaacatctta ttgaatgtga cgatcatgat actgcccagc aaattatcat cgatggcctg
     7501 aaacgccagt acgacgatcg cctactgctg ccgattcctc gactgaaaac caacaatccg
     7561 gaacagctgg aaaaagtgct gcgccagcag atcaaaaacg tcggcgatcg cccgctgttg
     7621 tggagcacac tgggccagtc actgatgaag cacggagaat ggcaggaagc atcgctcgcc
     7681 ttccgcgcag cgctgaaaca acgtccggac gcctacgatt acgcatggct tgccgacgcg
     7741 ctggacagac tgcacaagcc ggaagaagct gcagcgatgc gtcgcgacgg tttgatgtta
     7801 acgttgcaga acaacccacc acagtagttc cttctcaccc ggaggcaagc acctccggga
     7861 cctttctgat gcataaaaaa acgcctgctc ttattacgga gcaggcgtta aaacaggtct
     7921 gtatgacaac agtgggtgct tcactcaacg ttgtgtccat ggtgtctgat gaggcataag
     7981 cgacatctgt cagtggacga taagcaccgt aaacggctct gcgtcattcc ggagtttatg
     8041 aggcactaag gcgaacataa gagatggaat gagcatctac ccgtttatta tgccacagag
     8101 aatcgggaaa taacatccct taacacttgt tatgagataa ttctgtaatc ctctttgctt
     8161 cctgagtgaa tatttaacct gagcttgatc ctacacatac ttattatgaa tgataaaatt
     8221 cattcaatta ataacacata tattaattgc cgttaaaact aaaaaagcat taataatcaa
     8281 cgcaatataa taaacccgcc ttacatatca actgcgccag aggtaggatt gaaaacgctc
     8341 tcctgatttt ccaattcatt ttctggataa ataaataatt tatttttgtc actattattt
     8401 atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta cgtcatcatt
     8461 ttgatgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca aacttcttgt
     8521 tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc cgtcaactgc
     8581 caccgcacgc aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc
     8641 aatttattga atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa
     8701 tcaaccgcag gcggccacaa aatggccgct tttttactta ttcaccacca gcaaatgtgc
     8761 ctgcataatg tcactggccg ggcgaccgtg cgccagcaaa tcagccattg ctttaagata
     8821 tggcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt
     8881 gccgctggca tcgagcatga agcggtgttt cgggcagcct ccccagcacg cttttaacac
     8941 gttacaactg cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg
     9001 ttgcggggaa tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg
     9061 cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg
     9121 cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc
     9181 ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat
     9241 cgccaccaga aattcaccaa attcatcggg gcgcaccgac cattccgtca gttccccctg
     9301 aatatcacca ggcgcatgca atttcagtcc atcgcgggca gctgtttcat cagccagacg
     9361 ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac
     9421 ctgcaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg
     9481 atgtttttgc agcagcgtca gggcacgcat caccagctta tgcgttggtc tgccaccttt
     9541 ggtcacgcga tattgattgt ggatctcagc cggaccgtcc agcgataacc caacaagaaa
     9601 atgattttct gccagaaatg cacaccattt atcatcgagc agcacgccgt tagtctggaa
     9661 gctgttactt atcttcctgc cagcaccgta tttcgcctgt aactttacgg cacggcggta
     9721 aaaatccagc ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
     9781 cggttcgctg gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg
     9841 cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
     9901 cagattacaa tcggaaccac tcggtttcgc catcacatga aaagcacgcg ttggaacctg
     9961 ttgcagcatg gtcactcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata
    10021 ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag
    10081 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
    10141 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
    10201 ctttttgaat ttttagttca attctttaaa gtcgtggtgc gagggggggg gacttgaacc
    10261 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
    10321 gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga
    10381 atcacaatcc agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg
    10441 tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac
    10501 ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc
    10561 cgttgcgggg ggataatacg gacttcccac ccctctgtct agtccctttt taaataaatt
    10621 gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc tccagtttat gcgctttccc
    10681 gtggcgatta aggccaaaaa ctttataaat cgccgtcacc gccagcataa agatgatgcc
    10741 aacaaacagc gacatacgcg tatcttcatt aaagtacatg ccgatcaaaa cgcaaatcag
    10801 gaatgccatt gttacgtagt tggcccacgg gaacaggatt gagcggaacg gatggctggc
    10861 aatcgccgct ttatgtgcac gccgaaaacg cagctggctt atcaatatca caaaccatgg
    10921 caccatcccc ggaagcacgc tggcactgta gacgtagaca aacacacgct gcggattggg
    10981 aatgatgtag ttcaggcatg agccaattag cagaatagca atagatactg ccacacccgc
    11041 aactggtacg ccgtgacggg aaactttcgc catcgccgcc ggtaactgac ggtttttcgc
    11101 cagtgcgtag agcatacgtc cgcaactgta catgccgctg ttacagccag agagcgcagc
    11161 cgtcagcacc acaaagttga taatgcccgc cgctgcggta ataccgattt tggcaaaagt
    11221 cagtacgaac gggctgccgt tgctgccaat ttcattccac gggaagatgg tgacgataac
    11281 gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc actttgccta cagcactgcg
    11341 cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg ccaatcagct ccacgccctg
    11401 gtaggacgcc accacaatac acagcgcggt caggaaccct ttccagccac ccgcaaagaa
    11461 accgccatgc tctgtgagat tgctaaaacc aatcgactgc ccgccattgc caaagccaaa
    11521 gaaaatcacg cccaggccaa tgacaatcat cacgataatc gtggtgactt tgatcatcgc
    11581 gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc agattcgcca acgccaccag
    11641 cgccaccgcg atcaatgcag gtatccactg cgccatctcc gggaaccaga actggacata
    11701 aacgccaatg gcggtgattt ctgagatccc caccgccatc cacataaacc agtaagacca
    11761 ggcggtgaga taaccaaaaa acgggctcat ataacgatgc gcataaacgg cgaacgaacc
    11821 ggtaacaggt tcaaggaaca acatttcgcc cattgaacgc atgatgaaaa agacgaacag
    11881 cccggcgatg atataggcca acaatacgga tggcccggcc catttcaggg tactggcggc
    11941 ccccataaac aggccgacgc caatagtgcc ccccagggcg atgagttcga tatgtcgagc
    12001 ttccagccca cgctgtagct ctggtttgtt atctgccata aatcctcgtg ttgtgtttgc
    12061 atgctttccg gtgttaccgg ttatcgttat gggtacatcg agtgttgcga atgttttcgt
    12121 aattcaggag aaatggcaaa taaagcatta aaaatttgaa tgctttgtgt aataaaaaag
    12181 cagacaggcg acggagtgac cactccgtcg ctttacaaag agaggaaaat cagaggttgc
    12241 cggtgtagtg ccagcgtaaa taacgcagca aacgaagctg acgcttaatg cggctcggct
    12301 gcgaaagcag gcggtagagc cactccagtc ccagcgtttg ccagattttc ggtgcgcgtt
    12361 ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc catatacagc gcatctggat
    12421 gcaccagacg gcagtcgcgc atgaagatct cctgctttgg cgatcccatc gcaacggtga
    12481 cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc ctgacgctgc tcgggtttaa
    12541 aataaccatc ctgactgcca acgatattca cattccattg gttgcgcagt ttagcttcag
    12601 tttgcgccag cacttcaggt ttaccgccca caagaaatac cggcgtccct tctttgcctg
    12661 cgcgcgccat cagctcttcc cagagatcgg caccggcaac gcgggaaacc tgcgcctgcg
    12721 ggtacttttt acgtactgaa cgtacaacgc tgatgccatc cgcatattta aattcggcag
    12781 cgttaattaa ctccctgacc tcggcgttat cttcaatggt cagcattttt tcagcattaa
    12841 tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa cagataatcg agggcgtgct
    12901 gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt ataggtcggt gccgtggtgt
    12961 tgttattcat tgttatcctt caacctgcgt ccggagcgat gattttgtac gtttatgaat
    13021 gagtccggcg ctttcaaaaa gccagtacag cagttttgcg atcatcagac atgcgccgaa
    13081 gaccacgata aaaaagacta cgcgtgagac aaacgaatcc agcccttcac gcgccagcac
    13141 gatcatattg aagatcgccc caaagcagaa actgtgcaat atcgcagcct tatagcgatt
    13201 aggctcgcgg ttgcccagct catacagcca gtcgaaccat ttgatgatca gaccaaccac
    13261 gatcgccccg agcgggatga acaacgcgcc gcccatcacc accagtgagc ctataagcgt
    13321 aggcgagatc gccagtccgg agtggttatt cagcacttcc caggtaaagt agttggctga
    13381 gttcagcacc atactcgggc gacccggcca cagccaggaa gggataaaga catagaaatc
    13441 gcggacaatt ggagccaggc cctggaagtc gatgttgtcg tagttctgca acagcaacgc
    13501 cagattctcc cacggggaga aggtgtcgcg agtgagatag agaaacgtat agaacgcttc
    13561 atcgccgctc acattcattc catagcgttt tagcgccagc cagaacatgc caacaatccc
    13621 cagcacgccc gccgccgcca gcatccacaa cgaaatccag ccgcgaataa tgccaataaa
    13681 caggaagata gcgaatgcga tgatgatatt ggcgcgagtg ccgccgacaa tcatataagt
    13741 cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc cacgctttgc tgtcctggcg
    13801 cagaaagtag accaccagca tcgccgggat gaaaaagtaa aagaagcgtt ttaacgccac
    13861 gccggagact tcactggaga agatctgact gtaggagtta agccggaaca gcaaaaagcc
    13921 gttgtgcatg aagaagatgc cgacgcttac cagcgcgata cccatcagga tcacccacgt
    13981 aagattggtc tccacgcggt tcatggtaaa cagcggacgg cgcggtacat cagcaacgcg
    14041 tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg tagaagcagc ccgcagaaag
    14101 caacgcctgc aacaagattt ctggcggcgc gacaccaaca tcaaagcgaa ataccagcac
    14161 gctggtcagc gggaagccga agaaaaaggt gagcaaaaac agcaatgaaa agaagacatt
    14221 gaagttaaag cgcacacggc gaaactcaaa ccaggtcagc gtggcaataa acagcgtgca
    14281 gagcagccag accacaaaca ggccactgaa ttgcagcaga ctcatgcgac ctccccggcg
    14341 gcaatagcca acgcccgctg ccagccttgt agatagttag ggctaaagaa ggcaatggtg
    14401 tttttatcca ccgacgccaa ctggcgctgc gcttcacgca caatatcctc gttgagatcg
    14461 tcggtagtaa acagcaccgg caaatgctgt tccgtcatat cctgccagaa cggattttcc
    14521 cggttaagca cacaaggaat gcccgcctga atcagtaagc aaagcgtacc aatgccctgc
    14581 tggcgggcaa aaataaagta accaagatcg cactgacgaa gtagcgccag ataggcgtca
    14641 aattccagtt tttcgctcaa aatttgcaga ttttcttcgc tgaataactc cagccccgcc
    14701 tgacgaactt cctcaatgta cgcttcgtta ttaggcggat atcccatcgg caccaccact
    14761 tttaccgtat cgccaaattg ctgatgaacg gcgcgcaagg cagcaatatg gtcattgctg
    14821 cggtcaccgg agttccccac cagaatggtc attttccctt cacgttgccg atcattcgcc
    14881 atcgtattga gagaagggtc catccgcgtc gggaagtaca gcagttcgcc ccgcaccttt
    14941 ggatgcgttt tggcaaaaaa gctcaaatca ccgcgggtgg caaatacaca gccgactcgc
    15001 ttttgcgcca ggcgacgtag cgggtaaaaa agcttatatc tcaagccact ggaaagctca
    15061 tacaggtctg ccccccagat atgccagaaa aactggctgg gcttaatgcc accactcagc
    15121 agagccagcc acagtgtggg attgaactga ccgtggaaaa aaaatcgctg ctgacggtta
    15181 gcttttgctt tcgcgatgac cgcttccgcc agcgattttt tctcagggaa aaattgcaca
    15241 gaaagcgccg gacagctatc acttaagccg tcgtccttgc caacaaccat aaactcgcgc
    15301 gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc gcaaaacggt tcggttatgg
    15361 tgagggatat ccgatcccag tacgtgaatc agtacagtca tgcccgccta cgccagagta
    15421 aaaacacgcc acaacaaaga gaaaaataga cgatataagt tgccatatat gcctgcgccg
    15481 cgcccagtgc accatgcgca gggattagcc agtgggcaaa taccatcaat aaagtgaact
    15541 ggctgacttc cgccagaata taaaaccgca gtgacgcttt ggcgatcacc agataaccaa
    15601 agacataagc gcccactttt aacacatcac ccactaactg ccaggcaaag agatcgcgca
    15661 tagcggtaaa tttattcgac aacagcaacc agatagcaaa atcacgcagc aaccagacgg
    15721 taaaactcgc cgccgccact gccggtaaga cgaatttcag cgatttaacc acttcccggg
    15781 tgatatcgcg cttttccgtt agccgcgaca acgtgggcag caaatatacg ctgaacgatg
    15841 ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac cccttgccag atccccactt
    15901 catcccagct atactgcgcc gccagcagtt tacgcatcat gatgtaagca acaggcaagg
    15961 tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa ctgccctgcc agaccgttat
    16021 cccagctggg tttcagatag cttaacggga tgacaccacg tttgatcaac atgatggcgg
    16081 caggaatcac caccagcgcg ggaatcagcg ccagacccag caacgcccct tcataaccgc
    16141 ccaaacggta actgacgtag tacgcgagaa cgccaatcaa gctgccgaca atcagcgata
    16201 acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc cagtaacagg ttgccccagg
    16261 cgatccccat ttgcaccagc gccactaaac gcaccagccc ctgatagtcg gtattaccaa
    16321 acagtccctg gctgattggc gcagctgcca gcacaaaaac cagcgccatc agcgtagaga
    16381 aaccaagtac catcgctgat gaagtgccga ccacgcggcg cagctgttgc ggattatcat
    16441 ggtactgggc aacgtatttg gttacaccgt taaagatgcc agctccggca agcacgccga
    16501 gcacggtaat caactggcgg aaatttgccg ccagcccaag ccccgccgga ccaaatgaca
    16561 cagccagcaa cttaccgacc agtaacccgg caccaatctt gaccagtgta ctggccgccg
    16621 tccacaagga cgcttttgcc aacgacatat caggagaagt agttcaacaa agtcgcaatt
    16681 accgtacgct gattgacggg cgacaggttg tagaacagcg gcaggcgcag caggcgctcg
    16741 ctctctttgg tggtgtagcg atcttcaccg tggaactcac caaagcgttc ccccgcaggg
    16801 caaccgtgca gcggaatgta atggaacacc gccatgattt ccgcttcttt cagaaagtta
    16861 atcaacgcgc tccggtcatc aatatcccgc agtttaatgt agaacatatg cgcgttctgc
    16921 acgcagccat cgggaatcga cggcagctcg atacgcccgg ctttcgccag aggcgctaac
    16981 gcatcgtagt agttttgcca cagcgccaga cgttgctggt tgatacgatc cgctgcttcc
    17041 agttgcgccc acaggtacgc agcttgcaga tcggacatca aatagctgga gccaatatcg
    17101 cgccaggtat atttatcgac ctgaccacgg aagaactggc tgcggttagt gcccttttca
    17161 cggatgatct cggctcgttc gattaacgct ttatcgttaa tcagcgtcgc gccgccttca
    17221 ccacccgccg tatagttttt ggtttcatgg aagctaaagc agccaatatg accaatggtt
    17281 cccagtgcac gccctttgta agtggacatc acgccctgag cggcatcttc taccacaaac
    17341 agattatgct ttttcgccaa cgccataatg gtgtccattt cgcaggccac acccgcgtaa
    17401 tggaccggca cgataacgcg cgttttgtcg gtgatcgccg cttcaatcag cgtttcgtcg
    17461 atgttcatgg tgtccgggcg aacatccaca aaaacgattt ttgcgccacg cagcacaaag
    17521 gcattggcgg tggagacaaa ggtgtagctc ggcatgatca cttcatcgcc aggctggata
    17581 tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg acggcgtcag taacactttg
    17641 gcgctgccaa aacgttgctc cagccactgc tggcagcgac gggtaaaacc gccatcgcca
    17701 cacagtttgc cgctacccat tgccgactgc atatagtcga gttcggttcc caccaccggc
    17761 ggtgcgttaa atggaatcat gtgatcacct gtataaccag tacgcggtgc tttctacatt
    17821 cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg cccatttggg tcgccacccg
    17881 caaagttgtt ttaccgcgag catacgccca gtttagcgcc gtttgcatca gctcagcacc
    17941 tgcaccgcgt ccagccagca ggccaattcg cgcatctgtc gcattgagtt cccgtaaaga
    18001 gacataaccg cgaataccgc cggacgccgc acgtaaaatc agacattgat gatcaaaggt
    18061 gccgcgcacg gcattttcaa tccactgtgc ataaaagcgt ccgctggcgt caggcgcata
    18121 ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg gctaactgac gtaatgcggg
    18181 aatatcggtc tcttgtgcca ctacagcacc actatcactg acattgttca cgggtagtgc
    18241 caaatcaact tcaccttcta ccagggagaa tcccagctgt tgcagggcat ccagttcacc
    18301 cgtatttgat gccgcaattt tggcctgcac ccgtgaccac ggcgctaaca cgtctggcgt
    18361 caggagctgt gcttcagacg taatgcgcac gatggcgctg ttaacaccaa agaaggcgtt
    18421 ttcccaggtt agtggctcaa tactggcgcg gacgggcacg aagtaactcc agcagatatt
    18481 ggccgtagcc agttttcgct aatgaactgg cagcacgctt cacaccctcg tcatcgagcc
    18541 agccgttacg ccaggcgatc tcttccaggc aggcaatctt aaagccctgg cgtttttcta
    18601 ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg agtgccagtg tccagccagg
    18661 caaatccgcg cccgagcagt tcaacggtca ggttgcccgc ctcgaggtac atctggttga
    18721 tggaggtaat ctccagttca ccacgctccg acggcttcac ctgctttgcg tactccacga
    18781 ctttactgtc gtagaaataa agcccggtca ccgcccagtt tgacttcggc tgttttggct
    18841 tttcttccag cgagatagcg cggaaattgt cgtcaaactc caccacgcca aagcgttccg
    18901 ggtccatcac ctgatagcca aataccgtcg ccccttccgt gcgcgccgca acatgacgca
    18961 gcttcggact gaagccctga ccgaagaaga tgttatcgcc cagcaccaga caagaaggtt
    19021 caccgttgag gaaggtttca ccgatgataa aggcctgcgc cagaccgtcc gggctgggct
    19081 gttcggcgta ttccagctgg ataccgaact cactaccatc gcccagcagg cgctggaaat
    19141 aacctttatc ttccggcgta gtgatgatga gaatttcgcg gatatcggcc aacatcagca
    19201 ccgacagcgg atagtaaatc attggcttat cgtaaatcgg caacagttgc ttcgatacgc
    19261 cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc caggataata cctttcatgt
    19321 ttgcctccgc tgaaaattag cctttcagac ctaaacgctc gccctgatag ctgccgtcct
    19381 gcacctgctt ccaccagctt tcattagcca gataccactg caccgtttta cgcattccac
    19441 tttcaaaggt ttcctgtggc agccagccaa gttcacgggc aattttcgaa gcatcaatgg
    19501 cataacgcag atcatgcccc ggacggtcag cgacaaaggt gatcaagtca cgatagtgca
    19561 ccacgccgtg cggcttgttc ggagccagtt cttccagcag ctcgcaaatg gtttccacaa
    19621 catcgagatt cttacgctcg ttgtgaccac caatattata ggtttcaccg actttcccgg
    19681 tggtcgccac gcaatacagc gcgcgggcgt gatcttccac atacagccag tcacggattt
    19741 gctgcccgtt gccatatacc ggcagcggtt tacccgccag cgcgttgagg atcatcagcg
    19801 ggatcagttt ttccggaaag tggtaaggac cgtagttatt cgagcagttg gtgataagcg
    19861 tcggcagacc gtaggtccgc agccaggcgc gcaccagatg gtcgctgctg gcttttgacg
    19921 cggaataagg gctgctcggc gcatacggcg tggtttcggt gaagaaatca tccgtcgagt
    19981 gcaggtcacc atatacttcg tcggtggaga tatgatgaaa acggaacgct gattttttat
    20041 cttccgtcag cgtattccag taagcccgcg ccgcttcaag caatgtataa gtcccgacaa
    20101 tgttggtttc aataaacgct gccgggccgt caatagaacg gtcaacatgg ctttcggctg
    20161 ccagatgcat gacacagtct ggctgatgct cagtgaatac gcgtgccagt tctgcccgat
    20221 cgcagatatc aactttctca aaggcaaagc gctcgctttg cgcgaccggt gccagcgaca
    20281 tcaggtttcc ggcgtaggtc agcttatcga ccactaccac cgcgtcgctc gtttcgttga
    20341 tgatataacg caccagcgcc gagccaataa acccggcacc acctgttatc agaatttttc
    20401 tcatcagcgc cagactcctt tggcatcgac gacatactgc tgatggacat tgtcgccatt
    20461 gataacttta aactgactat gatcgaccag catcaccagc acgtctgccg ttgccagcgc
    20521 ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt ttcggcaact ggtggatgtt
    20581 aggctcaaca accagagttt cgccgctatg ccactgggcg atcagttcag cgatttccat
    20641 cgccgggctt tcgcgcaggt catcaatatt cggtttaaac gccagaccaa agcaggcgat
    20701 tttcagttca ctggcgcgtt tatcggtagc cgccaggcaa tcagccaccg ccgctttcac
    20761 ctgatcgata acccagaacg gtttgtgatc gttcacttcg cgcgcggtac ggataagccg
    20821 cgcctgctgg gggttctgtg ccacgataaa ccacggatca acagcaatgc agtgaccgcc
    20881 cacgccaggg ccaggctgaa gaatattaac gcgagggtga cgattcgcca ggcgaatcag
    20941 ttcccagacg ttaatcccct gatcggcaca aatcagcgac aattcattag caaaagcgat
    21001 attcacatcg cggaagctgt tttcggtgag cttacacatt tccgccgtcc gcgagttagt
    21061 gacgacacac tcaccttcga ggaaaatttt gtacagttcg ctggcgcggg tcgaacaaac
    21121 cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa cctggcccgg
    21181 taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc tgctcgccca cctgctgtgg
    21241 gaaagtgaga tccggacgca tctctgctaa ccattctgcc atcttctcgg ttgaccccac
    21301 cggcgaggtg gattcaagga tcaccagcgc gccttttttc agcactggcg caatggagcg
    21361 agcagccgat tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg gcgtgggtac
    21421 agcaatcagc caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa aaccgccttc
    21481 tacggcagtt tttactacgc tcgccaaatc aggctcgacg atatggattt cgccacgatt
    21541 gatggtatca accgcgtgtt ggttgatatc gacaccaatc acctgttttt gccgtgaggc
    21601 aaacgctgct gctgtaggca gcccgatgta acccagtccg ataacagaaa tggtcgcaaa
    21661 actcatagtg atatccgatt attttttaac gcttccagaa tgcgagagca tgcctgacca
    21721 tcaccatacg ggttatgggc gcggctcata gtttgatatt cgttttcgtc ttttaaaaga
    21781 cgcgtcactt cctcgacaat tcgctgctta tccgtgccta ccagacgcac cgtacccgcc
    21841 gtcaccgctt ccggacgctc agtggtatcg cgcatcacca gcaccggttt ccccagcgaa
    21901 ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca gccaggcgtg gttcatcagc
    21961 cagacaaacg gtaaatactc ctggggatcg atcagaatga catttttcac atgccccaga
    22021 atgcgattga ccggctctct gacgttcggg ttgagatgca ccggatagac aatctggatg
    22081 tcctggtgcg tggtggcaat gtctgccaac gcgtggcaga tttcttcaaa gccacgaccg
    22141 aaactttcac gcctgtgacc ggtcaccaga atcatctttt tatcggggtc gataaacggg
    22201 taatttgccg ccagttctga acgcagcgta tcgctgctca tcacctggtc acgcacccat
    22261 aacagtgcat caataactgt attaccagta atgaagattc ggctatccgc aacgttttca
    22321 cgcagcaagt tttgccggga agtttcggtt ggagagaagt gatacatcgc cagatgcccg
    22381 gtcaatgtac ggttagcctc ttccggccac ggcgaataaa gatcgcccgt gcgcagacca
    22441 gcctcaacgt gaccaacagg aatacgctga taaaacgccg ccaggctggt tgccagcgtc
    22501 gtcgttgtgt cgccgtgaac cagcacgacg tctggtttga actcggcaag aataggtttt
    22561 agcccttcca gaatccgaca ggttatctct gtcaggccct gtcctggctg cattatgttg
    22621 agatcgtagt caggtacaat ggaaaagagt ttcagcacct gatcgagcat ctcccgatgc
    22681 tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt ttgccaacgc atgcaccaac
    22741 ggcgccatct tgatggcttc cgggcgcgta ccaaatacag tcagtacttt cacatcgatt
    22801 ctcttcgaat aagcggcgag cgcctttgcg ctcaccgcag cagtgttgct atttcgagca
    22861 acggcgggtt aatgcgacac cagccccgat cagccccccg acaatgcccc acataatcat
    22921 caggaaggca cgacgtgggc tatcgcgttt taccggttct tccggcgtac gcaaatagcg
    22981 ataggtctga aaacgcggat ccagggttgg accaacattc agggtgttta acatggcccg
    23041 attctgatca tagtcgagat caaaggccgg accgacggcc tgtaaatttt ccagtcgagc
    23101 ctggagcatt ggacgcccaa gcaggaacat ttctgaatca ggtaattcct cggcaggcac
    23161 atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct gttcaatact
    23221 gttcatccgg cggtcgtaga tggctttcgc cacctcttcc tgacgcttca cctgtgcttt
    23281 catctggatg gtacgcgccg cccatgcgcc tttcagctca tcattcagat ggctggctgc
    23341 acgctggctg gcaaaagcaa catactgacg taacaggtta ttagcgtcag gcgcggtttc
    23401 agcaataagc ttcacgctgt cattgaccgc gcgggtaaag tctccgggga taaactggat
    23461 gttgttaatc atttcatcca gcaacgccgc atcggctttg ctgttgccca ccatccgctg
    23521 tttgtaatag tcggtttgca gccagaactc tctgcgggta tcccacgagg ccagttgcat
    23581 aacaaactct ttataggctt cgtccatgac cgatggttgg tcggcagaag ccatgtttga
    23641 acggacatcc aggttacgca aaaattgctg ctgcgagtaa tatcccccca gcatattcac
    23701 cgttggacga tcggtaatcg ccgtcgagct ccactcctga cgagcaaaaa aagtatacgc
    23761 cagcgcgatt aacgcaaatg ccagccccat gccaataatc catagcttcc cagcccacaa
    23821 ggtacgaaac aacccacgaa tatccagttc attttcagcg tcttcggccg gtttcccagg
    23881 cattggttgt gtcatcacat cctcatttat ttggttaaat tggggctgcc accacgattt
    23941 ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa ctttccaggc acgcttaatg
    24001 caatatccat agaggaagaa tgctagcaaa aagagcacca gcatgaccca ctccgggaca
    24061 aaatgagaat attctgccag cacgccaatg gaagcgagca gtgccgcagc aagggtaatc
    24121 agcacaaacg cctgacggga agtaaacccg gcacgcatga tcaaatggtg aatatgctga
    24181 cggtcaggag agaatgggct catgccttta cgcaggcgac ggtacataat cgccaccata
    24241 tccattagcg gaatggcgat tatccacaaa gcggtaaccg ggctgatggg atgggttttg
    24301 ccctgggtcg tttcgagcag gatccagata acggtaaaac caatcagcgt actgcccgca
    24361 tcacccataa agactttgta gcggcgaccc aggataccaa ggttaagcat gatgtatggc
    24421 aggatggcgg cgatcatcgc aaagcaccag attgcgaggc tggtttgccc gtcgaaccac
    24481 aaaatcatac cgattgctgc aaacgagacg caggacaacc cgcccagcaa gccatcaatg
    24541 ccatcaacca tgttgaacgc attaatggcc gcccagacgg caaatagcgt caggaagtaa
    24601 ccaaacggtc cgagcaccat ctcccaggag ccaaagatat aacccaggct actgagataa
    24661 agtttgccga acaccatcat aacaatgcca acagcggcct gtatggtggc acggattttt
    24721 acgctgatat caaaacggtc atccagcgcg ccaatgaaaa caagcacacc ggcacaagcg
    24781 agatagagag atgcatgcgg aatatagtaa tcgacaattc cgaacgtgaa gcaaatccct
    24841 gcgtaaaccg aaatcccccc aacgagaggt atcaatccct ggtgacgttt gcggaagttt
    24901 ggtttatcca ctaaaccgac tttttttgcc accttacggg caaaaaacag aaacagtgtc
    24961 gtgaataaaa aaatactgat gagatcagta ctcactgtca gtaaattcac aatgcatgct
    25021 ctcagagaaa attattagca gaagtataac cacgaagacc tttattcaga agggaaagtc
    25081 cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt gatatgcaaa ttgcataaca
    25141 cgccagcaaa ttccaggaat tttcctaaca tcgccagcgc ggcattaaga ttacagatat
    25201 aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat aagacaaatt tatgagcgtt
    25261 tcatcatttc gaagaaatcg tcattggtct tggtcatggc cagtttatta atgaggaatt
    25321 ccattgcatc gatttcgccc atcgggtgaa tgattttgcg caggatccac attttctgca
    25381 gttcttcctg agtagtgagc agttcttctt tacgggtacc ggaacggttg tagtcgatag
    25441 ccgggaagac gcgtttttca gcgatcttac gagagaggtg cagttccatg ttgcctgtac
    25501 ctttaaactc ttcgtagata acttcgtcca ttttagaacc ggtatcgata agcgccgtcg
    25561 cgataatagt caggctgccg ccctcttcca cgttacgcgc cgcaccaaag aagcgtttcg
    25621 gacgatgcag ggcgttggca tccacaccac cggtcaacac tttacctgac gccggaacaa
    25681 cggtgttgta agcgcgcgcc agacgagtga tggagtcgag cagaatgata acgtctttct
    25741 tgtgctcaac caggcgtttg gccttctcga tcaccatttc cgcaacctga acgtggcgag
    25801 atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt taccagacgc tgcatctcgg
    25861 ttacttcttc cggacgttcg tcgatcagca gaaccatcag cacacaatcc gggtggttgt
    25921 aagcaatgct ctgggcaatg ttctgcagca gcatggtttt accggctttc ggcggtgcca
    25981 caatcagacc acgctgacca cgaccgatag gtgatgccag atccagtacg cgagcagtta
    26041 aatcttcagt agaaccgtta ccacgttcca tacgcagacg agaatttgcg tgcagcgggg
    26101 ttaagttctc aaagaggatt ttgttgcggg cgttttcagg tttgtcgaag ttaacttcgt
    26161 taactttcag cagcgcaaaa tagcgttcac cttctttcgg cgggcgaatc ttaccagaga
    26221 tggtatcacc agtgcggagg ttgaaacggc ggatttggct aggggaaacg tagatgtcat
    26281 caggaccggc gaggtaggag ctgtctgcgg aacggaggaa accaaatcca tcctgcaata
    26341 tctccagtac gccatcacca aagatatctt cgccactctt tgcgtgctgc ttcaggatgg
    26401 caaaaataat gtcctgctta cgcatgcgag ccaggttttc cagccccata ttttcgccga
    26461 gagtgatcag ctcagaaacc ggcgtattct ttaattcggt aagattcata gtggtgtgag
    26521 ttcttaaact tggggtaaat ctcgaactta atgttgtgaa tggtatggca ggatcatcca
    26581 tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct ggtcacagga gagtacgcag
    26641 aactgaaacg acaagacgga ttgagtgacg agccagaaat ctgttcactt cgcatttaag
    26701 ataaaacggg aagcgttgga aattacaaga ttcaaactta ataaggtatg tttaatacga
    26761 agtcaacact aagttagcat gactcacgcc gggcgtccag tttttagcga cggggcaccc
    26821 gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac tctttcaact gacctttaga
    26881 cagtgcgccc actttggttg ccgccacttc accgtttttg aacagcagca gagtcgggat
    26941 accacggatg ccatatttcg gcgcagtgcc agggttttga tcgatgttca gttttgcaac
    27001 ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga atcggggcga tcattttgca
    27061 cggaccgcac cactctgccc agaaatcgac gaggatcgcc ccgtccgctt tgagtacatc
    27121 cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg ctcatatata actccacagg
    27181 aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt aaccaactaa aggttgactt
    27241 tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg atattctacc acactatgag
    27301 caaaacacat ttaacagaac agaagttttc cgacttcgcc ctgcatccga aggttgtaga
    27361 agcccttgaa aaaaaagggt ttcataactg tacgcccatt caggcactgg cccttccgct
    27421 gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc ggtaccggga aaacgatggc
    27481 gtttcttacg tcaacgtttc attatcttct ctctcatcct gcgattgccg atcgcaaggt
    27541 gaatcaaccg cgtgccttaa ttatggcacc gacgcgtgaa cttgccgtgc agatccatgc
    27601 cgacgcagaa ccgctggcgg aagctactgg cctgaagctg ggtctggctt acggtggtga
    27661 tggctacgac aaacagctga aagtgctgga aagcggcgtt gacattctga ttggcaccac
    27721 gggtcgttta attgactacg ccaagcagaa ccacattaac ctcggtgcca ttcaggtagt
    27781 ggttctggat gaagccgatc gcatgtacga tctgggcttt attaaagata tccgctggct
    27841 gttccgccgt atgccgcctg caaaccagcg cctcaacatg ctgttctccg ccacgctttc
    27901 gtaccgggta cgtgaactgg cgttcgagca gatgaacaat gccgaatata ttgaagtgga
    27961 accggaacag aaaacgggcc accgtattaa agaagagctt ttctaccctt ctaacgaaga
    28021 aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg ccagacagag cgattatttt
    28081 cgccaatacc aaacaccgtt gtgaagagat ctggggccac ctggcggcag atggtcatcg
    28141 tgtcggtttg ttgacaggcg atgtcgcgca gaaaaaacgt ctgcgtattc ttgatgaatt
    28201 tacccgtggc gatctggata ttctggttgc caccgacgtt gccgcgcgtg gtttgcatat
    28261 tccggcagtg acgcacgtct ttaactacga tttacccgat gactgtgaag attacgttca
    28321 ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac tctatcagcc tggcgtgtga
    28381 agagtatgca ttgaatttgc ctgctattga aacctatatt ggtcactcaa ttccggtaag
    28441 caaatacaat ccggacgcat tgatgaccga tctgccaaaa ccgctgcgcc tcacgcgccc
    28501 acgtacaggc aatggtccgc gtcgtactgg cgctccgcgt aatcgtcgtc gttcaggtta
    28561 aataaaatgt catactcaaa atcattcaaa ctgtatcaag atggcaagag aatgaatccc
    28621 gatgagcata ctgtagtaag tgattcaggt gaaagaatgc agccaacaca gagacagatt
    28681 gaaggatgaa gagtatgggt tccacctcgt cgctgtatgc agccattgat ctcggttcga
    28741 atagttttca tatgctggtt gtgcgcgagg tggctggaag catccagacg ctgacgcgaa
    28801 taaaacgcaa agtgcgtctg gctgctggcc tgaatagcga aaatgccctg tctaatgaag
    28861 caatggagcg cggttggcaa tgtctgcgcc tgtttgctga acgtctgcaa gatatccctc
    28921 cctcgcaaat tcgcgttgtc gctacggcga cattacgtct tgccgtcaat gcgggtgatt
    28981 ttattgccaa tgcgcaggaa atcctcggtt gtccggtaca ggtgatcagc ggtgaagagg
    29041 aagcacgtct gatttatcag ggcgttgctc acacaactgg cggtgccgat cagcgcctgg
    29101 tggtggatat aggcggtgcc agtactgaac tggtaaccgg cacgggtgca caaaccacct
    29161 cgttgttcag cctgtcgatg ggctgcgtca cctggctgga acgctatttt gccgatcgta
    29221 atctggggca ggaaaatttt gatgctgcag aaaaagcggc acgcgaagtg ttacgtccgg
    29281 ttgccgatga attacggtat cacggctgga aagtgtgcgt tggtgcttcc ggcaccgtgc
    29341 aggcgttaca ggaaatcatg atggcacagg ggatggatga acgcattacc ctggaaaagt
    29401 tgcagcaatt gaaacagcga gccattcatt gcggtcggct ggaggaactg gagattgacg
    29461 ggctgacgct ggaacgtgcg ttagtgttcc cgagtggtct ggcgatcctg atcgccattt
    29521 ttaccgaact gaatattcag tgtatgaccc tggcgggcgg tgcgctgcgt gaaggcctgg
    29581 tctacggcat gttgcatctg gccgtcgagc aggatattcg cagccgtacg ctgcgtaata
    29641 ttcagcgccg ctttatgatc gatattgatc aggcgcagcg cgtagccaaa gttgcggcta
    29701 acttcttcga tcaggtggaa aaggaatggc atcttgaagc aataagccgc gatttgctca
    29761 tcagcgcctg tcagcttcat gaaatcggcc tgagcgttga ctttaaacaa gcgccgcaac
    29821 acgctgctta tctggtgcgt aatctggatc ttcccggttt tacccccgca cagaaaaaat
    29881 tgctggcgac gctactgctc aaccagacta atccggtcga tctctcatcg ctgcatcagc
    29941 aaaatgccgt accgccgcgc gtcgcagaac aactctgccg tttactccgc ctggccatca
    30001 tttttgccag ccgtcgccgt gacgatctcg tgccagagat gacattacag gctaaccatg
    30061 aactgttgac cttgacgctt ccgcaaggtt ggctaaccca acatccgctg ggtaaagaga
    30121 ttattgctca ggaaagccag tggcagagct atgtccactg gccgctggaa gtgcattaat
    30181 gagtaagtgc cggatgcgat gctgacgcat cttttccggc cttgattatt tccctcgttt
    30241 tgccgccatc atcgctttca gattcgccag atggctttgc cctttctgca tccgttcttc
    30301 ggcgctgacc actttgcgct cctgttccca aatcagatca tcctgcggca gctccagcaa
    30361 aaagcggctc ggctccgggc gcaccagctc gccgtactga cggcgttctt tacacagcgt
    30421 aaaggtcaat tccttctggg cgcgggtaat gccgacatag gccagccgcc gctcctcatc
    30481 gatattatct tcatcgatgc tgctctggtg cggcaaaaac ccttcttcca taccgaccat
    30541 gtagacataa ggaaactcca gcccttttga cgcgtgcaga gtcatcagtt gcacctgatc
    30601 cagctcttct tcactctcac cacgctccat catgtcgcgc aaagtaaagc gcgtcaccac
    30661 ctgggtgagc gtcatcggct catccagttc gctgccttcc agcatctccg tcatccagct
    30721 aaacagttgg ttgacgttct tcatgcgcat ttcggcggct ttcgggctgg gcgatgtttc
    30781 gtacagccag gattcataat ccatgccgtg gatcagatcg cgcaccgcgg caatcggctc
    30841 ccgctccgcc agacgctgga tttctgccaa ccagtgagta aagcgggtca atgcttcata
    30901 accacgtccg ctaagcgtct gactcaggcc catatcaaag ctggcggtaa acatgctttt
    30961 attgcgcgtc atcgcccact cacccagctt tttcagcgtc gccgggccaa tctctctctt
    31021 cggcgtgtta acgatacgca gaaatgcgct gtcatcgtcc gggttagtca gcacgcgcag
    31081 ataagccagc aagtccttga tttcaggacg agagaaaaac gacgtaccac cagaaatttt
    31141 gtacgggatg cggttttgca tcaggaattt ttcaaacact cgcgactgat ggttaccgcg
    31201 ataaagaata gcgtaatctt tgtactgcgt tttattgacg aagtgatgcg caatcagttc
    31261 accagtgacg cgctcggctt catgttcttc gttattcgcg cttaaaactt taagctctgt
    31321 gccgtaaccc agttcggaga acagacgctt ctcaaagacg tgcgggttat tggcgatcag
    31381 gatgttcgcc gctttcaaaa tacgtccgga agagcggtag ttctgctcaa gcttaatcac
    31441 cttcagcgcc ggaaaatcct gactcagcag caccaggttt tgtggacgtg cgccgcgcca
    31501 ggagtagatc gactggtcgt catcgcccac cacggtaaag cgcgcgcggc tgcccaccag
    31561 cagtttcacc agttcatact ggctggtgtt ggtgtcctga tactcatcca ccagcagata
    31621 gcgaattttg ttctgccagc gctcgcggac ttcttcattg cgttgcagca gcaacgtcgg
    31681 caataaaatc agatcatcga agtcgagaac attgcacgct ttcaggtgtg catcatacag
    31741 cccataacaa tgggcaaaaa tacggtcccg ctcgccaatc gcactggctg ccgcctggga
    31801 cggtgttttg agatcgttct tccagttaga gatggtcgaa atcaactgtt gcaggagaac
    31861 tttgtcatct tcaatcagcc cttcggtcaa ctctttgagc aacgccagct gatcggtatc
    31921 gtcaaacagc gaaaagttcg ccttcatccc cagcgccgca tactcacgct tgatgatatc
    31981 cagccccagc gtgtggaagg ttgagatcat cagcccacgc gcctctttgc gacccagcgt
    32041 ctgtccgaca cgctctttca tctcgcgcgc cgccttattg gtaaaggtca ccgccgcgat
    32101 atgtcgcgcc tgataaccgc aaccgcggat cagatgggcg attttattgg tgatcacacg
    32161 agttttaccg gaacccgcgc ccgccagcac caggcagggg ccggtaacga attcgacagc
    32221 ttgttgttgg ccggggttta gacgcatagg tgtattgctc aatcttcgaa cggggggagg
    32281 attgtagcag aatcgcattt gaatgtgacc gcgtgtaagc cgctaaccga ttggctgcgg
    32341 atgtttaaat cagagaggga agaccgaaac cggagttcat cgtgaatcca ttagacttag
    32401 aaaatatcgg gtcgtaacgt tgtgttatta ctttttcaga gtttactaag gagaggtgta
    32461 ctcgtggcga ttaatttcag tcctaaggtt ggtgaaatac tggattgcaa ctttggtaac
    32521 tatcctgtta gccagaatgg acagttcagc accacgtatt acgatggccg cattcctcct
    32581 gaaatgataa aaaacaggtt agttgttaga ctggccccct gaatctccag acaaccagta
    32641 tcacttaaat aagtgatagt cttaatacta gtttttagac tagtcattgg agtacagatg
    32701 attgatgtct tagggccgga gaaacgcaga cggcgtacca cacaggaaaa gatcgcaatt
    32761 gttcagcaga gctttgaacc ggggatgacg gtctccctcg ttgcccggca acatggtgta
    32821 gcagccagcc agttatttct ctggcgtaag caataccagg aaggaagtct tactgctgtc
    32881 gccgccggag aacaggttgt tcctgcctct gaacttgctg ccgccatgaa gcagattaaa
    32941 gaactccagc gcctgctcgg caagaaaacg atggaaaatg aactcctcaa agaagccgtt
    33001 gaatatggac gggcaaaaaa gtggatagcg cacgcgccct tattgcccgg ggatggggag
    33061 taagcttagt cagccgttgt ctccgggtgt cgcgtgcgca gttgcacgtc attctcagac
    33121 gaaccgatga ctggatggat ggccgcc
//
LOCUS       DAJVYM010000032        33124 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVYM010000032 DAJVYM010000000
VERSION     DAJVYM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.032"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..643)
                     /locus_tag="OQS60_001396"
     CDS             complement(<1..643)
                     /locus_tag="OQS60_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS2387186.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="OQS60_001397"
     CDS             complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="OQS60_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS2387187.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="OQS60_001398"
     CDS             complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="OQS60_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS2387188.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="OQS60_001399"
     CDS             complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="OQS60_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS2387189.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="OQS60_001400"
     CDS             complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="OQS60_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS2387190.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="OQS60_001401"
     CDS             complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="OQS60_001401"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS2387191.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7180..8415
                     /gene="moeA"
                     /locus_tag="OQS60_001402"
     CDS             7180..8415
                     /gene="moeA"
                     /locus_tag="OQS60_001402"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS2387192.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8415..9164
                     /gene="moeB"
                     /locus_tag="OQS60_001403"
     CDS             8415..9164
                     /gene="moeB"
                     /locus_tag="OQS60_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS2387193.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="OQS60_001404"
     CDS             complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="OQS60_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS2387194.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10136..11035
                     /locus_tag="OQS60_001405"
     CDS             10136..11035
                     /locus_tag="OQS60_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS2387195.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11041..13473
                     /locus_tag="OQS60_001406"
     CDS             11041..13473
                     /locus_tag="OQS60_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS2387196.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13619..14434
                     /gene="ybiV"
                     /locus_tag="OQS60_001407"
     CDS             13619..14434
                     /gene="ybiV"
                     /locus_tag="OQS60_001407"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS2387197.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14586..15851
                     /locus_tag="OQS60_001408"
     CDS             14586..15851
                     /locus_tag="OQS60_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS2387198.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15818..16978)
                     /locus_tag="OQS60_001409"
     CDS             complement(15818..16978)
                     /locus_tag="OQS60_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2387199.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17216..18517
                     /locus_tag="OQS60_001410"
     CDS             17216..18517
                     /locus_tag="OQS60_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2387200.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18532..20895
                     /locus_tag="OQS60_001411"
     CDS             18532..20895
                     /locus_tag="OQS60_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCS2387201.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21075..22670)
                     /locus_tag="OQS60_001412"
     CDS             complement(21075..22670)
                     /locus_tag="OQS60_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS2387202.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22889..23809
                     /gene="ldtB"
                     /locus_tag="OQS60_001413"
     CDS             22889..23809
                     /gene="ldtB"
                     /locus_tag="OQS60_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS2387203.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23868..24986)
                     /locus_tag="OQS60_001414"
     CDS             complement(23868..24986)
                     /locus_tag="OQS60_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS2387204.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="OQS60_001415"
     CDS             complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="OQS60_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS2387205.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25636..25764
                     /gene="mntS"
                     /locus_tag="OQS60_001416"
     CDS             25636..25764
                     /gene="mntS"
                     /locus_tag="OQS60_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS2387206.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26036..27619
                     /gene="opgE"
                     /locus_tag="OQS60_001417"
     CDS             26036..27619
                     /gene="opgE"
                     /locus_tag="OQS60_001417"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS2387207.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="OQS60_001418"
     CDS             complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="OQS60_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS2387208.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28236..28301)
                     /locus_tag="OQS60_001419"
     CDS             complement(28236..28301)
                     /locus_tag="OQS60_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387209.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28536..29423
                     /gene="rhtA"
                     /locus_tag="OQS60_001420"
     CDS             28536..29423
                     /gene="rhtA"
                     /locus_tag="OQS60_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS2387210.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29722..30225
                     /gene="dps"
                     /locus_tag="OQS60_001421"
                     /gene_synonym="pexB"
     CDS             29722..30225
                     /gene="dps"
                     /locus_tag="OQS60_001421"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS2387211.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30629..31375
                     /gene="glnH"
                     /locus_tag="OQS60_001422"
     CDS             30629..31375
                     /gene="glnH"
                     /locus_tag="OQS60_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS2387212.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31514..32173
                     /gene="glnP"
                     /locus_tag="OQS60_001423"
     CDS             31514..32173
                     /gene="glnP"
                     /locus_tag="OQS60_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS2387213.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32170..32892
                     /gene="glnQ"
                     /locus_tag="OQS60_001424"
     CDS             32170..32892
                     /gene="glnQ"
                     /locus_tag="OQS60_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS2387214.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         8067 a         8976 c         8295 g         7786 t
ORIGIN      
        1 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
       61 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      121 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      181 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      241 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      301 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      361 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      421 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      481 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      541 ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta tctgaacgct
      601 gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa
      661 aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct
      721 ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac
      781 catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag
      841 cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg
      901 gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg
      961 tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc aagatatgac
     1021 gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct
     1081 gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg gaaaaaatgg
     1141 cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca
     1201 gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc
     1261 agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca ccgataaaca
     1321 cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt
     1381 cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt
     1441 cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca
     1501 caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc
     1561 gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc atggcgttta
     1621 acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt
     1681 aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag
     1741 cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg
     1801 tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc
     1861 aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc cgtgtttgag
     1921 gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa
     1981 aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg ccgcgccgag
     2041 cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc
     2101 cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat
     2161 ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc
     2221 gataattccc gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca
     2281 cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga
     2341 caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg
     2401 ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgagccc
     2461 ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga
     2521 gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc
     2581 actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg
     2641 atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg
     2701 atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc
     2761 gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt attgctgtaa
     2821 aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg cgatagcgcc
     2881 cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct
     2941 ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg
     3001 gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg
     3061 ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc
     3121 agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg
     3181 atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc
     3241 agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc
     3301 ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc catcaactcg
     3361 atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc
     3421 gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt
     3481 atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca
     3541 aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca
     3601 atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca
     3661 agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg
     3721 atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga
     3781 tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg
     3841 aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa
     3901 ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa
     3961 aacgatttcg ctacggcctg agataacgta tcattcgcgt catacggatc gagcgtggtg
     4021 aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac
     4081 gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc
     4141 tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg
     4201 ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt
     4261 gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac
     4321 cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg
     4381 ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg
     4441 ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag
     4501 atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc
     4561 gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg
     4621 ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg
     4681 ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg
     4741 cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc
     4801 ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc
     4861 gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc
     4921 ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg
     4981 ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa
     5041 accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc
     5101 caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa
     5161 cgggaaacgt cggggataat ccaacccttt catcgcacca agttgcggaa cagcagctaa
     5221 cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc
     5281 caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat
     5341 atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg
     5401 caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac
     5461 cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag
     5521 ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat
     5581 ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc
     5641 ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat
     5701 catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc
     5761 aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc
     5821 ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc
     5881 aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat
     5941 tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc
     6001 atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg
     6061 gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga
     6121 cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt
     6181 ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg
     6241 cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccgata caagaaaccg
     6301 ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc
     6361 cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat
     6421 ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat
     6481 ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg
     6541 tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc
     6601 cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg
     6661 gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca
     6721 cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa
     6781 acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc
     6841 ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta
     6901 attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa
     6961 tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata
     7021 tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga tatggcatgt
     7081 aaaggcagga gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat
     7141 aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg
     7201 atgtcgctcg acaccgcgct taatgagatg ctttctcgcg tcaccccact gaccgcccag
     7261 gaaacgctgc cactggtaca gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg
     7321 cttgatgttc cggggtttga taactccgca atggacggct acgcggtgcg tttagccgat
     7381 attgcctccg ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccgtatcat
     7441 ggtgaatggc ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc
     7501 tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact
     7561 gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg
     7621 gttgttttcc cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg
     7681 gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat
     7741 gaactccagt tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc
     7801 gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc
     7861 gacgatcccc atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg
     7921 atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag
     7981 ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt
     8041 aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc
     8101 ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg
     8161 cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat
     8221 ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga
     8281 catcagggtt cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa
     8341 cgcgatcgcg gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg
     8401 ttcggaggcc tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa
     8461 tcattctgcg cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctga
     8521 tcgtcggcct cggtggactc ggctgcgcag cttcgctgta tctggcaagc gccggtgtcg
     8581 gcaacctgac gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac
     8641 tgcacagtga tgccacggtc gggcaaccga aggtggaatc cgcccgtgac gccctgacgc
     8701 ggatcaaccc acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg
     8761 cagcattgat tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta
     8821 atcaactgaa cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc
     8881 gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc
     8941 ttagccgttt gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt
     9001 tgatcggcgt aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg
     9061 gcaaaccgac cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa
     9121 tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc
     9181 tgataagacg cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa
     9241 cgccttatct ggcctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact
     9301 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
     9361 aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct
     9421 aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacatcctg ccagtaagca
     9481 gtccagcgcc tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg
     9541 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
     9601 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcaacccttg
     9661 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
     9721 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
     9781 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
     9841 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
     9901 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
     9961 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    10021 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    10081 aatctaactt tcattcgaaa gtattttaat gtttgaacga aacagagagg cgactatgat
    10141 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    10201 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    10261 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc
    10321 gccggaagtg attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc
    10381 ggagcatctg acggcgttaa ccgactgctg tccgacacag gcattaaccg tgtgtggtga
    10441 agtgaaaagc gttgaggaga tcatgacgac cgttctgcgc gataaaccgt tttacgatcg
    10501 cagcggcggc ggtttaacgc tttcgggtgg tgagcccttt atgcagccgg aaatggcgat
    10561 ggcgctactg caagccagcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    10621 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    10681 aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct
    10741 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    10801 tcagggcttt aatgccgacg aaacctctgt aaaagctatt accgattttg ccgccgacga
    10861 gctgcacgtt ggcgaaattc atttcctgcc ctaccacacg ctaggcatca acaaatatca
    10921 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag aactgctcga
    10981 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    11041 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    11101 gtgcatattg tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat
    11161 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg
    11221 gcgaatcgca ccatctggat caaacacgat gagttgatca ttggcaacca ggcaagcgaa
    11281 gttcgcgccg cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat
    11341 gatctggcag atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgcgttctg
    11401 catgaagtgt gcccgtggtg gcgcggtcag accgtacagg atcgctgcta cggcatgttt
    11461 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    11521 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    11581 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    11641 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    11701 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    11761 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    11821 ttctggcagg cgctgcaact gtgttacttc atccaattga tattgcagat cgaatctaac
    11881 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    11941 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    12001 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    12061 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    12121 gacgcggtga atccactctc ttacgctatc ctcgaatcct gcggtcgcct gcgttccact
    12181 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    12241 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    12301 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    12361 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    12421 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa
    12481 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    12541 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    12601 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    12661 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    12721 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    12781 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    12841 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    12901 gacgatgata ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    12961 aaacagtacc ataacccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    13021 acctcgtcta tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac accggacggg
    13081 cgtaaagccc acaccccgct ggcagaaggc gcaagcccgg cctccggtac tgaccatctt
    13141 ggccctactg cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc
    13201 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa
    13261 ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac
    13321 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    13381 gtagtgcgtg tcgcgggcta ttccgccttc ttcaccgcgc tctctccaga cgcacaggac
    13441 gatatcatcg cccgtactga acatatgctg taatctcctc ggcccggcgg cgtcgtcgcc
    13501 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    13561 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    13621 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    13681 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt
    13741 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    13801 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    13861 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    13921 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    13981 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    14041 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    14101 gatcgacaaa ctgcacatag cgctcgatgg cattatgaaa cccgtcacca gtggttttgg
    14161 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    14221 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    14281 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    14341 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    14401 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    14461 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    14521 tttataactt atattgtcgt atgcaaatca gaatggtttc gaatgaaagt taatgagagg
    14581 tggttatggc ttctactttt accagcgaca cattgcctgc cgatcacaaa gcagctatcc
    14641 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    14701 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg
    14761 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    14821 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    14881 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    14941 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    15001 ccatctactg gtcgcaggcg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    15061 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    15121 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    15181 gtcttggagc gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc
    15241 gcgttttcgc caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac
    15301 atcgtacgga agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga
    15361 cattccaagg ctggacagcg ctctctaata tgctgcctgg tcaggggctg ctgcacgttg
    15421 tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg
    15481 aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc
    15541 cactgttaat tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt
    15601 ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg
    15661 tgatgtacat tcctgcggca ccaatgtgcg agaaaaatct tgcctacgcg cacaaggtga
    15721 aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa
    15781 actgggaagg acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggaa
    15841 tcgacagctg agtgtcgtac agcccttccc gttccacggc ggtgttccct tttcctgggc
    15901 gaacccgccg cactgactcc cgcaccgcca atcgtccgtg cagcagcagc gagcccaccg
    15961 gagcatccgg gcgaatcagc cgctgttgca acatttgcac cgcttcattg cctagctcat
    16021 cgcgtggcac atgcacagcc gtcaggggaa catcctcgat tgccgcaagg ttaaagccgt
    16081 cgatactcat caccgagaca tcctgtggta cgcgcagacc acgacgatgt agcgcattta
    16141 ccgttcccgc cgccataaaa tccccgccga ccagaaacgc cgtcggcaac gtcttgccat
    16201 tggcggcatc cagccacgct ccgacctttt cttccgcttc tttagcgcta aaactgctga
    16261 tattcagcaa atgacggtca tggtgaaagg ccagattatg ctgacgccat gattctttaa
    16321 tccctgcaaa ccgctgctcc atagtgtagc ggcgcaggca catcacattc atcacctccc
    16381 ggtgacccat ttcaaacaga tagcgggcgg cgaacgcgcc aattaaccga tgatccggcg
    16441 caatcgccgg gaggcgcatt cgttcatcac ggcagttgat cagcacacaa ggcttagcga
    16501 gatcggcggc cagatcatgg atgtgcggat cgtcaatccc cagcaggatc gctgcctgcg
    16561 tatctgcttc attcatccgc gagagaaaga gcgcgggatc gctgtcaaac tcctccagcg
    16621 cgcagtaacg cagccgcact tcatgcggcg ataacgcttt gctgatgctc tggatcacac
    16681 ggtaataaaa gatatcagat cgttggtcaa aagcgcgttg cggcgcgaaa ataatcaggt
    16741 tgttcagcaa catacgacct gctgccatgc catccatcac gcccaactca cgcgcgcagg
    16801 ccagcacctg ttcgcgagcg cgtgcactgg tattcgcttt gccagccagc acccgcgaaa
    16861 ccgtactcgg cgatagcccg gtacgtgcgg cgatatcggc gattttcagc ttcttgtcca
    16921 ttttgtgatc tgcctctaat tcggaaatga cagaattttc atgagcaaaa aagcccattc
    16981 tgtctggcat taaatccatt tcagtaatgt atatcagtca ctcatggaaa aacgtgcaca
    17041 aaacccgctt tctttcgctt ttagggtctc ttactgtgaa ttggtagaac aacttccata
    17101 caaaaataat ggaaaaacga acaataaaga ctttatttat caaaaagtta aaagtggtgt
    17161 aacaactcca ttctgacgtt gccgcacaaa ccctatacca ttctgtggag tgcatatgag
    17221 tcagggtatc aataacgata tgactgccag taaaccgcgc catatcgtca aaaatctgcg
    17281 ttggtggata ctggtccttt ttttgctcgg tgttactgtc aactacatca cgcgtaactc
    17341 gctgggtatt ctcgcccccg aactgaaaag cagcctaggg attaccaccg aacaatattc
    17401 ctggattgta ggtgctttcc agctcgccta taccctattc caaccgttgt gcggatggct
    17461 gattgacgtc attggcctca aactgggctt tatgatctgc gcgggactgt gggcactggc
    17521 ctgtctgatg catgcggggg cgggaaactg gggacatctg gcgctgttgc gcttctttat
    17581 gggcggtgcg gaagccgcgg caaccccagc aaacgccaaa accatcggtg aatggtttcc
    17641 aaaatcagaa cgcccgatcg ccgcaggctg ggcaggcgtc ggtttttcca tcggtgccat
    17701 gctggcccca ccgattatct attttgccca cgcttcattc ggctggcagg gtgcatttat
    17761 gttcactggc atcctggcga tgctgtgggt ggtgctgtgg tgggcgttct atcacagccc
    17821 ggataagcat cctaacctca gcaaagccga gctggacttt attcgccagg ataacgaagc
    17881 gcccccggtc aagttaccct ttctgaccgc actgaaaacc gtgggcaaaa ataaacgctt
    17941 ttacggtatc gctatcccgg cctttatggc agaaccggcg tgggcggtga tgagtttctg
    18001 ggtgcctcta tacctgtcca aaacctatgg catggacctg aagcagatcg cattattcgc
    18061 ctggttgccg ttcctcgccg ctgatcttgg cagtattgcc agcggctatc tgaccaaact
    18121 ttacacccgc ctgtttgggc tgagtcgtgt gaactccgtt gtcgcatcat cggtcagtgg
    18181 cgcattcttg atggtttcgc tggcggtgat ggcctttacc aataacccgt atatcgccat
    18241 cgtccttatc tccatcggcg gcttcggtca ccagatcatc tcctgcatgc tgagcgcact
    18301 ggtggtggaa tccttcgaca aaggtcagat ggccactgtt aacggcatgc gcggttctgc
    18361 cgcgtggatc gcaagtttcc tgttctccct gattatcggg gttaccgctg acaaaatcgg
    18421 ctttaacccc ctgtttatcg ccatgggctt ctttgacctg attggcgctt tcttcctggt
    18481 cacctttatt gctgaacgtc gcgcaaaacg cgcctgagag tggatgtaaa catgaaaacg
    18541 ctgaaaaact ggaccctgca caaacagtct gcccaccacg ttgaactgct ggtcgatggc
    18601 cagcatatcc tgtgcctgta cgtgctggaa gaaaatctgt tccgcgtgct gttaaaacgc
    18661 aaaggcgagc tggcgctaga tcgcacctgg agcattgctc cggcgcagga tgtgccatgg
    18721 gaagggcgca gccgcgaaga tctcagcggc tttagcctgc ccgcgtggac agtgatccag
    18781 cacgacgaca ccgtggttat cgccaccgag tcactgcgtg caaccgttca ccagccgctg
    18841 tggctggagt ggcactatcg taacaacgaa ggcgaatggc tgccgttggt gaatgaccgc
    18901 ccgaccagcg cttatctgct gaatgcccac ggcgatggcg tggcacacta tcagagccgt
    18961 cgtaaagatg aacgctttta tggcctcgga gataaagccg gcgatctgca acgcaacggt
    19021 aaacgttacg agatgcgtaa cctcgacgcg atgggctaca acgccgttag tactgacccg
    19081 ctgtacaaac acattccatt caccatcacc cgccgcgatg acattagctt cggcctgttt
    19141 tatgacaacc tcagcagctg ctggctggat ctgggcaacg agatcgacaa ctaccacacc
    19201 gcctatcgcc gctggcaggc ggaagcgggt gatatcgact actacctgtt taccggcgaa
    19261 cgcgtacttg atatcaccaa agccttcgta cgcctgaccg gaaaaaccct gttcggaccg
    19321 aaatggagcc tgggctacag cggttcaact atgcactaca ccgatgcgcc ggacgcacaa
    19381 aatcagttga tgaactttat ccgcctgtgt gaagaacatg caattccctg cgattcgttc
    19441 cagctctctt ccggttatac ctcaattaac ggcaaacgct atgtgttcaa ctggaactac
    19501 gacaaagtgc cgcagccgaa agtgatgagc caggcatttc acaatgctgg tttgcacctg
    19561 gccgccaata tcaagccgtg cctgttgcag gatcacccac gctataacga agtggcagaa
    19621 agaggactgt tcatccgtga ttcagaaaca gatgaaccag aacgttccag cttctgggat
    19681 gacgaaggtt ctaacctcga gtttactaat ccgcagacca tccagtggtg gcaggaaggc
    19741 gtgaccacac agcttctgga gatgggtatc gactccacct ggaacgacaa caacgaattc
    19801 gaagtatggg acggtgaagc acgctgtcac ggctttggtc agacgatcgc gatcaaacat
    19861 attcgcccag tgatgccact gctgatgatg cgagcctcga tggaagccca gcagcgtttc
    19921 gctccggaaa aacgtccgta cttgatctcc cgctccggct gcgcgggaat gcagcgctac
    19981 gtgcagacct ggagtggcga taaccgcact agctgggaca ctctgcgcta caacacccgc
    20041 atgggtctgg ggatgagcct ctccgggctg tataacctcg gtcacgacgt cggcggtttc
    20101 tccggtgata agccggaccc ggaactgttt gtacgctggg tacaaaacgg cgtgatgcat
    20161 ccgcgcttta cgattcactc atggaacgat gaccataccg taaacgaacc gtggatgtat
    20221 ccgggcgtca ctccggcgat ccgcagcgct attgagttgc gctatcgcct gctgccctac
    20281 ttttataccc tgatgtggca ggcatacgcc gatgatgaac caatgctacg tccgactttc
    20341 cttgaccacg agcacgacgt tcagactttt gaagagtgtg atgacttcct gcttggtcgc
    20401 gatattttgg ttgccagcgt cgtagagcaa ggcgagcgcc agcgccgtgt atggctgcca
    20461 gataacgaaa ctggctggta cgatttctac aacggcgaat ggttcagtgg cggtcagtgg
    20521 atcaccctcg acgcaccgct ggagaaactg ccgctgatgg ttcgtgccgg ggctggtcta
    20581 ccgctcagca aacgcatcac ctatgtgagc gcagaacaag acgatacccg cgaactgaaa
    20641 ctgttcccgc tgaaaggtgt gggcacaacg tccggcctgc tgtttgaaga cgatggcgaa
    20701 agctggggct accaaactgg taatgcgctt tgggtggaat gggaaatggt ttgcgatggg
    20761 gcgaccatca acctgaaagt caacgcgcgc ggggattatc gtcctgcatg gagtgcactg
    20821 aaagtgtcat taccattgga agaaaaacgc acgctgctgg tcaatggtgt tgaagggagt
    20881 gaatggatgc gttaaatcat taccggggcc gggaggcgac tgcaccttac ccggtccagc
    20941 catcgaatcc tttacctcag tatttcacgc tgcaggaagg cagcaaacct gtaaaccccg
    21001 ggagcttaaa taagtaagtg accggggtga acaggtgaag ccaatgcatc tgccgcatga
    21061 aagacggcgg ggaattaagc gtcgatccct ttactacgca ggtaatcttc gtaattaccg
    21121 ctaaagtcga tcgcgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    21181 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    21241 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    21301 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
    21361 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
    21421 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
    21481 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
    21541 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
    21601 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    21661 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
    21721 cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc
    21781 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    21841 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    21901 ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg
    21961 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    22021 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    22081 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    22141 agaatctccg gatcagcaaa cagcgcctgc gccagaagca cacgcagttt ccagccagga
    22201 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
    22261 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg
    22321 gccactttat agccgtcttc ctcactcatt tccggcaaag catagatgcg gtcacgctcc
    22381 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    22441 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
    22501 ttacccaaag tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta
    22561 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
    22621 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    22681 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    22741 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    22801 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    22861 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    22921 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    22981 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    23041 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    23101 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    23161 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    23221 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    23281 caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg
    23341 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt
    23401 cgttccggca ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta
    23461 cgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
    23521 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    23581 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    23641 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat
    23701 taccctgacg aagagcgtgc aaacagtgac cggtcagcca gatgttgacc aggttgttct
    23761 tgatgaagcg attaaaaacc gctccgggat gccggttcgt ctgaattaat cttcaaaact
    23821 taaagcaaaa ggcggactca taatccgcct tttttatttg ccagacccta gttggccggg
    23881 agtataacta acaaaacata tcccaccaac gccgcccaca acagcatcgg tattgaatag
    23941 agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat caaatttgcc
    24001 agcgatccgg gtaataaccc aaagccacct acgtttaccg cccataccag taataaagac
    24061 ggcggcacat agttcagcaa caatatggta ctcggcacgt tactgatcac ctgcgataaa
    24121 ccgattgccg ttaaccataa cccgggttca gatagatgac tcacgttacc caacacgcct
    24181 tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat aaacaccagc
    24241 agcagcgtcc agtccacact gagcaccacg cgacgtgcca gcagcgcaaa gcctgccgcc
    24301 acaatcacca gtccccacag ctcttgtttg aactccagcg ccgtcagaaa gacgatatac
    24361 agccccagac aactccacac cagccgcggt ttccactccg gtgtttgcac ccccgtatgg
    24421 tattgcagcg cctttccagg gaaacaacac cagcacagga gcaggagcgt cagcatcatt
    24481 gcgccagcca gcggtgccat ttgggcaata aatccggcaa acgaaagacc agaacgtccc
    24541 cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc
    24601 agcgcctcaa aaataatcag ccgattaacc gggatctcac acagtctttt tagcgtgata
    24661 gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc
    24721 gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg caccattttg
    24781 cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag catcaggccg
    24841 cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc
    24901 acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg atcgccttgc
    24961 agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag
    25021 cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga cgggcgattt
    25081 ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt
    25141 cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg atcatttcaa
    25201 tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc gaaactccca
    25261 gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag tcagaaatca
    25321 gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt
    25381 caacgtgctc ttccacgttc actaactgcg tcactttttt tgctgttggc gtacctgcgc
    25441 gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca tttcgtgcgc
    25501 acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat agctacataa
    25561 tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga cgggattagc
    25621 aagtcaggag gtcttatgaa tgagttcaag aagtgtatgc gcgtgtttag tcattctccc
    25681 tttaaagtac ggttaatgct gctctctatg ttgtgcgata tggtcaacaa caaaccgcag
    25741 caagataaac cttccgataa atagcggcgt cgcggtacgc cgcttcactc ctgcttttat
    25801 gcaggcataa cgcgttttgg tctgaaaaac cccacttttt gtcggatttg caatcccctt
    25861 cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat cttcttgcag
    25921 ccctacgtat attgcggctc gcggatgcgg acccctttcc cctcttcacg cactcttgca
    25981 ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt ggcatatgaa
    26041 tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt ggactgcgtt
    26101 ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca gtttgctcta
    26161 caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc gcgtacaaaa
    26221 agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg ctcaggccta
    26281 cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg aagagtcgac
    26341 cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta tttttgcgct
    26401 cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga ataccttcga
    26461 cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc aaaacctggc
    26521 ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt ttgctaagga
    26581 tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa aactttccgg
    26641 aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct atgtggtggt
    26701 gatcggcgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact gggacaatac
    26761 cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgccg ccagtggctc
    26821 cacgcagaaa tcacttggct taacgctcaa tcgcgtagtc gatggcaaac cacagtttca
    26881 ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt tttccaacca
    26941 gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg cagatgaagt
    27001 gtacttcctg aaagaaggta atttcgaagc agataaaaac acgaaagacg aagcgttact
    27061 ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga ttgttctgca
    27121 tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg aaacctttgt
    27181 gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg atttactgcg
    27241 caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt acttttctga
    27301 ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc atgatgataa
    27361 atatcagcaa aatttccagg tgccttttat ggtcatttcc agcgacgata aagcgcatcg
    27421 ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc agtggacggg
    27481 aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga aagccgggcc
    27541 gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg gaaccgatat
    27601 tttcgacccg aaaccttaaa acaaaaaatc cgccccgaga ggcggatttt ttatatcacc
    27661 aaagtgatta gaagcggtaa ccaacaccgg caatccaggt gcctacgtca acgctacgaa
    27721 tacggctctg ctcgtaagag aagtccagag caacgttttc catcgggttg aactgcagac
    27781 cagcaccgta ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg
    27841 tctggaattt accataaccc acccccacta caccgtagat gcttgcccag tcgttaatgc
    27901 ggtaagccgg accagcagtg atgccgtagt actggttttt gttgtagtca ccagagcttg
    27961 cagtacggct tttctcggtg taagtgaaag aaccgatcac acccagcggg ctgttgtctt
    28021 cttcatagcg gtatttcagg ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc
    28081 tctgtgcgta accgccagtt acggtagaag tcgcagctac ggaagtacct gcggtgaaag
    28141 ccagaactgc ggccagtgct gaaagacatg caattttttt cataaccacc tcaaatgtga
    28201 ttcaaataag tcctaagttt taaatatatc aaaaattaat gggaaactct tcgcgatttg
    28261 tgatgtctaa cgggccattt catgtaacag aacgtttcca tacaccgcta tccatctaaa
    28321 tttaaatcac tttttcagag aactgcgtaa gtattacgca tgttttccct gtcattcatc
    28381 cagattattc ctaatcacca gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa
    28441 cgggggaaaa ttttttcaac aaatgctcaa ccagcattgg gtatatccag tacactccac
    28501 gctttactta agtctagata tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc
    28561 cggtctggtt accaatagtc atattgctcg ttgccatggc gtctattcag ggtggagcct
    28621 cgttagctaa gtcacttttt cctctggtgg gcgcaccggg tgtcactgcg ctgcgtctgg
    28681 cattaggaac gctgatcctc atcgcgttct ttaagccatg gcgactgcgc tttgccaaag
    28741 agcaacggtt accgctgttg ttttacggcg tttcgctggg tgggatgaat tatctttttt
    28801 atctttctat tcagacagta ccgctgggta ttgcggtggc gctggagttc accggaccac
    28861 tggcggtggc gctgttctct tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg
    28921 ttcttggtct gtggttcctg ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg
    28981 gctgtgcgct ggcactgggg gccggggctt gttgggctat ttacatttta agtgggcaac
    29041 gcgcaggagc ggaacatggc cctgcgacgg tggcaattgg ttcgttgatt gcagcgttaa
    29101 ttttcgtgcc aattggagcg cttcaggctg gtgaagcact ctggcactgg tcggttattc
    29161 cattgggtct ggctgtcgct attctctcga ccgctctgcc ttattcgctg gaaatgattg
    29221 ccctcacccg tttgccaaca cggacatttg gtacgctgat gagcatggaa ccggcgctgg
    29281 ctgccgtttc cgggatgatt ttcctcggag aaacactgac acccatacag ctgctggcgc
    29341 tcggcgctat catcgccgct tcaatggggt ctacgctgac agtacgcaaa gagagcaaaa
    29401 taaaagaatt agacattaat taaatttaca tttctgcatg gttatgcata accatgcaga
    29461 atttctcgct acttttcctc tacaccgtct ttatatatcg aattatgcaa aagcatattt
    29521 attccgaaaa ttcctggcga gcagataaat aagaattgtt cttatcaata tatctaactc
    29581 attgaatctt tattagtttt gtttttcacg cttgttacca ctattagtgt gataggaaca
    29641 gccagaatag cggaacacat agccggtgct atacttagtc tcgttaatta ctgggacata
    29701 acatcaagag gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat
    29761 ctgctttata cccgcaacga tgtctccgac agcgagaaaa aagcaacagt agagttgctg
    29821 aatcgccagg ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac
    29881 atgcgcggcg ctaacttcat tgccgtacat gaaatgctgg atggcttccg caccgcactg
    29941 atcgatcatc tggataccat ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg
    30001 accactcaag ttatcaacag caaaaccccg ctgaaaagtt acccgctgga catccacaac
    30061 gttcaggatc acctgaaaga actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc
    30121 aaagcgattg gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc
    30181 gacctggata aattcctgtg gtttatcgag tctaacatcg aataaatcca tcgctgatgg
    30241 tgcagaactt tagtacccga taaaagcggc ttcctgacag gaggccgttt tgttttgcag
    30301 cccacctcaa cgcacttatt tagtgcatcc atctgctatc tccagctgat taagtaaatt
    30361 ttttgtatcc acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac
    30421 gctaaataat cactgtgttg agtgcacaat tttagcgcac cagattggtg ccccagaatg
    30481 gtgcatcttc agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa
    30541 atcaaggagt tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc
    30601 gtcccgtgga tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc
    30661 actgaccctg gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac
    30721 ggataccgcc ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg gctttgacgt
    30781 tgatctgtgg gctgccatcg ctaaagagct gaagctggat tacgaactga agccgatgga
    30841 tttcagtggg atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat
    30901 taccatcacc gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg
    30961 cctgttagtg atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg
    31021 gaaagtggtt gctgttaaga gcggtactgg ctccgttgat tacgcgaaag caaacatcaa
    31081 aactaaagat ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa
    31141 ccgcgcagac gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg
    31201 taacggtcag ttcaaagcgg taggtgactc tctggaagcg cagcaatacg gtattgcgtt
    31261 cccgaaaggt agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga
    31321 gaacggaact tacaacgaaa tctacaaaaa atggttcggt actgaaccga aataataacg
    31381 ctacacctgt aaagcgcact ggcagttccc tctcccctct ggggagagga ttagggtgag
    31441 gggcgcaaac ccgctccggg gccattaatt accctgaatt tgattattta caccacggta
    31501 acaggaacaa catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat
    31561 tgaaggtgcc aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg gtctggtaat
    31621 cggattgctg gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct
    31681 ggtctttatt gaggtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatttattt
    31741 cgccctgccg atggcgttta acgacttacg catcgaccca tttactgcgg cggtggtcac
    31801 catcatgatc aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat
    31861 tcacaaaggt tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg
    31921 ctacgtcatt ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat
    31981 catcagcatt aaagacacct cgctgtttat tgtgatcggc gtggcggaac tgacccgtca
    32041 ggggcaagaa attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt
    32101 gttctatctg attattaccc tggtgctaag ctttattttg cgtcgtctgg aaagaaggat
    32161 gaaaatcctg tgattgaatt taaaaacgtc tccaagcact ttggcccaac ccaggtgctt
    32221 cacaatatcg atttgaacat tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt
    32281 tccggtaaat cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ctccggcgat
    32341 ctgattgtcg atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag
    32401 gaagcaggta tggtgttcca gcagttttac ctcttcccgc atctgacggc gctggaaaac
    32461 gtcatgtttg gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg
    32521 cgtgagctgc tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt
    32581 tctggtggtc aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg
    32641 atgctgtttg atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag
    32701 gttatgcagg atctggctga agaagggatg acgatggtga tcgtgaccca cgaaatcggt
    32761 tttgccgaga aagtagcttc gcggctgatc tttatcgaca aaggccggat tgcggaagat
    32821 ggcgatccgc aggtattgat caagaacccg ccgagccagc gcttgcagga atttttgcag
    32881 cacgtctctt aacttctctg cctccctttc acccgaaagg gaggcacacc agattcctct
    32941 catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt tttctcactg
    33001 gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg
    33061 cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc
    33121 cgga
//
LOCUS       DAJVYM010000033        32530 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVYM010000033 DAJVYM010000000
VERSION     DAJVYM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.033"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(134..1210)
                     /gene="glpQ"
                     /locus_tag="OQS60_001425"
     CDS             complement(134..1210)
                     /gene="glpQ"
                     /locus_tag="OQS60_001425"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS2387215.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1215..2573)
                     /gene="glpT"
                     /locus_tag="OQS60_001426"
     CDS             complement(1215..2573)
                     /gene="glpT"
                     /locus_tag="OQS60_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS2387216.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2846..4474
                     /gene="glpA"
                     /locus_tag="OQS60_001427"
     CDS             2846..4474
                     /gene="glpA"
                     /locus_tag="OQS60_001427"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS2387217.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4464..5723
                     /gene="glpB"
                     /locus_tag="OQS60_001428"
     CDS             4464..5723
                     /gene="glpB"
                     /locus_tag="OQS60_001428"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS2387218.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5720..6910
                     /gene="glpC"
                     /locus_tag="OQS60_001429"
     CDS             5720..6910
                     /gene="glpC"
                     /locus_tag="OQS60_001429"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS2387219.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7103..8005
                     /locus_tag="OQS60_001430"
     CDS             7103..8005
                     /locus_tag="OQS60_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS2387220.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8046..8849)
                     /gene="yfaU"
                     /locus_tag="OQS60_001431"
     CDS             complement(8046..8849)
                     /gene="yfaU"
                     /locus_tag="OQS60_001431"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS2387221.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8867..10156)
                     /locus_tag="OQS60_001432"
     CDS             complement(8867..10156)
                     /locus_tag="OQS60_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2387222.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10213..11417)
                     /gene="rhmD"
                     /locus_tag="OQS60_001433"
                     /pseudo
     CDS             complement(10213..11417)
                     /gene="rhmD"
                     /locus_tag="OQS60_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11432..12214)
                     /locus_tag="OQS60_001434"
     CDS             complement(11432..12214)
                     /locus_tag="OQS60_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS2387223.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12434..13636)
                     /locus_tag="OQS60_001435"
     CDS             complement(12434..13636)
                     /locus_tag="OQS60_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS2387224.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13736..14278)
                     /locus_tag="OQS60_001436"
     CDS             complement(13736..14278)
                     /locus_tag="OQS60_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387225.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14557..14982
                     /gene="nudI"
                     /locus_tag="OQS60_001437"
     CDS             14557..14982
                     /gene="nudI"
                     /locus_tag="OQS60_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS2387226.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15021..15623)
                     /locus_tag="OQS60_001438"
     CDS             complement(15021..15623)
                     /locus_tag="OQS60_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS2387227.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15931..17070
                     /gene="arnB"
                     /locus_tag="OQS60_001439"
     CDS             15931..17070
                     /gene="arnB"
                     /locus_tag="OQS60_001439"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS2387228.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17074..18042
                     /gene="arnC"
                     /locus_tag="OQS60_001440"
     CDS             17074..18042
                     /gene="arnC"
                     /locus_tag="OQS60_001440"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS2387229.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18042..20024
                     /gene="arnA"
                     /locus_tag="OQS60_001441"
     CDS             18042..20024
                     /gene="arnA"
                     /locus_tag="OQS60_001441"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS2387230.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20021..20911
                     /locus_tag="OQS60_001442"
     CDS             20021..20911
                     /locus_tag="OQS60_001442"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS2387231.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20911..22563
                     /gene="arnT"
                     /locus_tag="OQS60_001443"
     CDS             20911..22563
                     /gene="arnT"
                     /locus_tag="OQS60_001443"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS2387232.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22560..22895
                     /gene="arnE"
                     /locus_tag="OQS60_001444"
     CDS             22560..22895
                     /gene="arnE"
                     /locus_tag="OQS60_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS2387233.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22895..23281
                     /gene="arnF"
                     /locus_tag="OQS60_001445"
     CDS             22895..23281
                     /gene="arnF"
                     /locus_tag="OQS60_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS2387234.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23275..23541)
                     /gene="pmrD"
                     /locus_tag="OQS60_001446"
     CDS             complement(23275..23541)
                     /gene="pmrD"
                     /locus_tag="OQS60_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS2387235.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23585..25006)
                     /gene="menE"
                     /locus_tag="OQS60_001447"
     CDS             complement(23585..25006)
                     /gene="menE"
                     /locus_tag="OQS60_001447"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS2387236.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25003..25965)
                     /gene="menC"
                     /locus_tag="OQS60_001448"
     CDS             complement(25003..25965)
                     /gene="menC"
                     /locus_tag="OQS60_001448"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS2387237.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25965..26822)
                     /gene="menB"
                     /locus_tag="OQS60_001449"
     CDS             complement(25965..26822)
                     /gene="menB"
                     /locus_tag="OQS60_001449"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS2387238.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26837..27595)
                     /gene="menH"
                     /locus_tag="OQS60_001450"
     CDS             complement(26837..27595)
                     /gene="menH"
                     /locus_tag="OQS60_001450"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS2387239.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27592..29262)
                     /gene="menD"
                     /locus_tag="OQS60_001451"
     CDS             complement(27592..29262)
                     /gene="menD"
                     /locus_tag="OQS60_001451"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS2387240.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29351..30646)
                     /gene="menF"
                     /locus_tag="OQS60_001452"
     CDS             complement(29351..30646)
                     /gene="menF"
                     /locus_tag="OQS60_001452"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS2387241.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30725..31030)
                     /gene="elaB"
                     /locus_tag="OQS60_001453"
     CDS             complement(30725..31030)
                     /gene="elaB"
                     /locus_tag="OQS60_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS2387242.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31085..31546)
                     /locus_tag="OQS60_001454"
                     /pseudo
     CDS             complement(31085..31546)
                     /locus_tag="OQS60_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31611..32528
                     /gene="rbn"
                     /locus_tag="OQS60_001455"
     CDS             31611..32528
                     /gene="rbn"
                     /locus_tag="OQS60_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS2387243.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8805 c         8139 g         7690 t
ORIGIN      
        1 cgttgttggc taaagaaaag ccggtagaag gcagtccact gtttttacta caaatgtgca
       61 tggaacaatt agtggcgatt ggaaaaattt gatatgagac aggatggcgc atgaacgcca
      121 tcctgcagta gcattactct ttattaagga attttactgc cttatcaggg aaatcagtaa
      181 acagcccatt tacacccgct ttgttataca gagcatcata taactgattc acatcagtag
      241 tgtattcagg cagtttatcc gaccgcacgg tataaggatg cactaccagt ttattctgct
      301 gagcatcttg caccatgcca gtgagtttga tattacccgg ctgcgatgtt tcctcaatca
      361 acatatggta atccggcccg ataccgtcgg catattccgc gacttgcttc atggcacctg
      421 gcttaaacat ccagtcatag ctgtaattaa cccagcttcc gtccggctgt ttctgctgcg
      481 tttcattcca gtcggtatag gcaatcagct gtaccagatt gagatccatg cccattttag
      541 gttccagctc attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt
      601 tatcgtcctt accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat
      661 ccttcccttc ctgatgatgg aaccacggcg ctttgatttc tggatagata ccgatatttt
      721 tcccggtaga gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgccccc
      781 ggaagtcgga cttacccatt gggaaacgcc ccggataagt ctgtactttt ttaccgtttt
      841 caatatcgaa accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg
      901 cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac
      961 gatcgagata atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct
     1021 gttccagata atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg
     1081 gcaaatatcc actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca
     1141 ttgcactgct tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagct
     1201 tcaatttcat gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt
     1261 tcgccaatca tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc
     1321 ataaagccgc catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc
     1381 accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt
     1441 ttcggtgcca gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag
     1501 ccgataacaa tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa
     1561 acgatagtcg cgatggtcac cagtgtcata aagaaaacgc cggttgcccc acggttgcca
     1621 cggaagactt tatccgacat ccagccgcac agcagagtgc ccggaatacc tgcatattca
     1681 taaaggaagt aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa
     1741 gtcggtgacc agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg
     1801 atataccaca gcagtttgtt cggcagtacg tactgcatga agatttgctt cgccgtcagc
     1861 tcctgttccg ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc
     1921 ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga
     1981 atggcgcaga aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc
     2041 agcaggaaca gcagcggcgg aataccacca ccgacgttgt gcgcacagtt ccacactgac
     2101 acaatgccgc cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc
     2161 caccccatcc cctggaacca accgcagagg aacaacagta caaacatcac cgcaatgctc
     2221 gacgtcgccc atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct
     2281 gcgggcagga aaacgcgcgg attcgagcga tccgataccg aacccatgat gaatttcgaa
     2341 aatccataag caatcgagat ccccgaaagg gcaaaaccta aatcaccgcg tgagaatccc
     2401 tgctcaacca gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca
     2461 tagccaaaga atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc
     2521 tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc
     2581 ctccgtggcc cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt
     2641 ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac
     2701 attattaaat aagatttaca aaatgttcaa aatgacgcat gaaatcacgc ttcactttcg
     2761 aatcatgagc gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta
     2821 aaaaacgaac ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat
     2881 tatcattggc ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct
     2941 gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg
     3001 cctgctacac agcggtgcgc gctatgcggt aaccgatgcg gaatcggccc gcgaatgcat
     3061 tagtgaaaac cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct
     3121 gtttattacc ctgccggaag atgacctctc cttccaggcc acttttattc gcgcctgcga
     3181 agaagcaggg atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc
     3241 cgttaacccg gcattgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg
     3301 tctgaccgca gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca
     3361 tgaagtcacg ggactgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca
     3421 tctcaccggc gaaactcagg cccttcatgc gcctgtcgtg gttaatgccg ccgggatctg
     3481 ggggcaacac attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc
     3541 gctgctgatc atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc
     3601 cgacgccgat attctggtgc ctggcgatac catttcgctg attggcacca cctctttacg
     3661 tattgattac aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct
     3721 gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc
     3781 tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg
     3841 catcgtgctg ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg
     3901 tggcaaactg atgacctatc ggctgatggc tgaatgggct accgacgccg tgtgccgcaa
     3961 actgggcaac acgcgcccct gtacgaccgc cgatctggca cttcctggtt cacaggaacc
     4021 cgctgaagtt accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt
     4081 ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa ggtcgtctgc accgtagcct
     4141 ggtatgtgag tgcgaagcgg taacagcggg tgaagtgcag tacgcggtag aaaatttaaa
     4201 cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg gggatgggta cctgccaggg
     4261 cgaactctgc gcctgccgcg ctgccggact gctgcaacgt tttaacgtca cgacgtccgc
     4321 gcaatctatc gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat
     4381 cgcctgggga gatgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg
     4441 tggtctggag aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg
     4501 ccggattact ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc
     4561 gtggtcaaag cgcgctgcat ttctcatccg gttcgctgga tttgctgagc catctgcctg
     4621 atggtcaacc ggtgacagac attcacagtg gactggaatc tttgcgtcag caggcaccag
     4681 cccatcctta cagtcttctc gaaccacaac gcgtgctcga tctcgcttgc caggcgcagg
     4741 cattgatcgc tgaaagcggt gcgcaattgc agggcagcgt ggaactggcc catcagcgcg
     4801 ttaccccgct cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc
     4861 cgctacccgc gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc
     4921 atcttgcggc ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc
     4981 tgccggaact ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc
     5041 gtttccttga taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca
     5101 atacctgcga aatgatcctg atgcccgcct gcttcggcct ggccgatgac aaactgtggc
     5161 gttggttgaa tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg
     5221 tgctgggcat tcgtctgcaa aaccagttac agcgccagtt tgtgcgccag ggtggcgtgt
     5281 ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct
     5341 ggacccgcaa tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca
     5401 gtttctttag tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc
     5461 ttgatgtgct acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc
     5521 aaccgtggca gcagttcggt gtaaccactg atgagacgct acgcccgtcg caggcagggc
     5581 aaaccattga gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc
     5641 aaggatgcgg cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc
     5701 aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt
     5761 ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc
     5821 cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa
     5881 atattgcatc aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga
     5941 tattatccag cgcgcgcggg caaaatatga caccacgcgc ccgtcgctgc gtaattttgt
     6001 gttgagtcat accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac
     6061 cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg
     6121 tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc
     6181 tcagcaagca caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa
     6241 ccatccgcag ttaggtaaag atttaattaa agtgctcaac gcaatgggta tcggtgtaca
     6301 actgctcagc aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa
     6361 agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg
     6421 cattccggtg attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga
     6481 agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct
     6541 gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt
     6601 ttatcacact ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt
     6661 gcgtaaaatc ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg
     6721 tacttacggt ttcaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt
     6781 ccgccagata gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg
     6841 gcagattgag atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc
     6901 gctggcttaa actcctttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta
     6961 cacgattccg tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc
     7021 gcaaaggctg aaactggcag atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat
     7081 aaatatcagg tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt
     7141 atttaaaacc tttatgttca cacccgaaac cgcacgggat tttctcgaaa tacatttgcc
     7201 agaaccactg cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat
     7261 tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg
     7321 tgacggctat atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt
     7381 tcggctaatg cgctatgcca ctgccgccat gcagcgtcac ctggataaag gctatgacag
     7441 agttccgctg gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc
     7501 acttaactgg ctggatgagt ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc
     7561 ttttccgttg gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat
     7621 agctctgctt gaactgattc aaaagcatat tcgcgaccgc gatttaatcg gtatggtcga
     7681 caggatcacc acgcttttgg ttaaaggctt cactaatgac agccagctac aaacactgtt
     7741 taattatctg ctgcaatgcg gcgatacctc ccgtttcacc cgttttattg aggagatttc
     7801 cgaacgttca ccactacaaa aggagagatt gatgactatt gctgagcggc tacggcagga
     7861 aggacaccaa attggctggc agaaaggtat gcatgaacaa gccattaaaa ttgctttgcg
     7921 catgctggaa cagggcattg atcgtgacca ggtgctcgcg gccacccagc taagcgaagc
     7981 cgatctggca gcgaataacc actaattaac acaggcccac agccgatccc catgggcctt
     8041 tgatatcaat aactaccttt tatgcgtggc ccatttttgc ctgatttaaa catcgccagt
     8101 cgttgatcca gggcatcgct gtagagcatc gtgtcaacgc caacagcgac aaagttcgct
     8161 ccccacgcca ggcattgctg cgccatatca ggagccacag ccagaaaacc agccgcttta
     8221 cccgcagcac ggatccgccg aatgctggtt tcaataattc gctgcacttc cgggtgcccg
     8281 gcgttatccg ggtagcccaa cgacgcagaa agatccgctg gtccaataaa cacgccatca
     8341 atcccttcga cgtcgaggat ctcgtccagg ttatccagtg ccgttttact ttccacctgc
     8401 actaacagac aaagcgaatc gttgacttgc gccatgtaat tctcaatgcg tccccagcgc
     8461 gcagcccgtg ccacactggc cccaacacca cgctcaccgt agggaggata gcgcgtggca
     8521 gacgccacct gacgagcctg atcggcagta tcgaccatcg ggatcaatag agtttgcgcg
     8581 ccaatatcca gaacttgttt aatcagcgat ttactgcctt ccaccggacg gatcacgggt
     8641 tggctggcat agggcgctac cgcctgtagc tggtgataaa gatcctgaat ggtgtttggc
     8701 gcgtgctccc cgtcaatcag caaccagtca taaccggaag tagcggcaat ttctgccata
     8761 taagaagtag tcgagcttaa ccacagacca atttgcactt cgcccttgcg taaacgttct
     8821 ttaaagggat tggttaataa tgcgttcatc gtgttcctta acctatttaa tgatgtgcca
     8881 cgtcggtctg cgcaacagta cgatttacac gcagagtgaa aataatcagc gaaccgatga
     8941 ccgccaccgc tgccagtgtc agtaatcctg ccgcatcgct ggcaaataac gtttctgctt
     9001 tcacgcgcag gatcggggca ataaacccgc ccactgcacc aaacaggttt acaaaaccaa
     9061 tccccgcagc cagcgccgta ccggaaagca gttgtgtcgg catcgtccag aacaccggct
     9121 gcacggcaat aaacccgata gctgcaacac acagcgctac gatcgccatt actggagaaa
     9181 gcagcccgga cagaccaata ccaatgcccg ccgccagtaa tgtcagcgct gcgacattac
     9241 gccgttcgcc ggttttatcg gaatagcgcg gaataagcca ggtcccaaac aaggccgcaa
     9301 cccacggaat ggcggtgacc accgacgctg taaagcccac ttttgttccc agcaatgccg
     9361 caacctgggt cggcaggaag aaaattaatc cgtaaaccgc cacctgaatg gtcaggtaga
     9421 taatcgctag ttgccagact cggccattac gcagcgcatc gctcagccga gaagtcactt
     9481 tctgttgttc ttcacttgcc agttgattga taagcaacgt tttttcttgt ttactcagaa
     9541 aacgtgcctg ctccggtgtg tcatcaagcc aaaagaatgt gaatacccca gcaccgactg
     9601 ccaacaatcc ttcaatcaca aacatccaga accagccggg atgccccata aatccatgca
     9661 tctccaacag cgcgccggaa agcggtgatc ccagtgttaa cgccagcggt gcgcccatat
     9721 agaacagccc cataatgctg gcgcgattac gctgcggaaa ccattgcgag gtgagataaa
     9781 tcataccagg gaaaaatccg gcctccgcag caccaagcag agtgcgaact atcagaaatt
     9841 tcgcttcagt atcggcccat gccatggctg ccgaaagaaa tccccacagc agtgttgtcg
     9901 taccaatcca ggttctggcc cccagtttgc gcatcaaaag attcgccgga acacccagaa
     9961 acgcatatac cacaaagaaa atgcctgctc ccagcgcata agcttcatta ctcaacccgg
    10021 tatcaatctg gtaggtctgt ttggcaaaac cgatattcga acggtcgaga aacgccagca
    10081 catacagcgc caacataaac ggaattaaac gcacacggtt tttcttcacc acggcgtcaa
    10141 gcaaagcggt gctcataaaa agctccttag aatatgtggg cgttaccgtt gtcacagcaa
    10201 cgcccggagt gattagtggc tgtaggggcg tttcaaattg cagtcacgat tgagttcgac
    10261 gccaaaaccg ggtttatcaa gcactgattt atgaatacga ccattcaccg gaaccggctc
    10321 attgagcaga atcgggtcaa actgcggacg catcgttgaa caatccgggc tggtcatcag
    10381 gaattcgctg aatggcgtat tggtgaaggt gatcaccgca tggtgagagt aaacagacga
    10441 accgtgcggc accaccagtt gccccctgga tttggcgatt gcggcaattt ccaccagcgt
    10501 ggttaaacca ccgcaccagc caacatccgg ctgcataatg tcgataccgg tttctgaaag
    10561 cgtacgaaaa gattgcagtg tgccgtggtg ctcaccgctg gtgaccatca ttccgactgg
    10621 cgcgttgcgt ttcagttcgc gataaccttc atactgctgt ggcggcaggc actcttcgat
    10681 ccatttcagg ttatagggcg cgcaagcgtg ggccagtttg gtggcatagt tcacgtcctg
    10741 actcatccag cagtcgagca ttaaccagaa atcctcaccg catttttcac gcatatccgc
    10801 gaccataacg gcatctttgc ggatccccgc atcgccatca tgtggccccc agtgcgtcgg
    10861 cattttgcca ccgataaagc ccatctcttt tgccagatcc ggacgcgcgc ctgtggcgta
    10921 gaactgaatc tcatcacgaa ctgcgccgcc taaaagttta taaaccggaa gcccgaccac
    10981 tttgccgaac agatcccaca gagccagatc gacacaggaa atcgtgttca tcaccaggcc
    11041 accagagccg gagtaataca gggtggcatt gagcatttga tcatggatca gtttgatatc
    11101 actgacacat ttaccctcaa tgaaacggtt aagatgtttt tcgacaataa aacagcccat
    11161 ttcaccggct gtcgaaacag cgaatccggt ctggccgttt tctgcttcga cttcaacaat
    11221 caacgtgcca agaacgttaa tgccaaatga ctggcgtgac tgctcgtaat cgcggtattt
    11281 actcatcggg gtggcaatat gatcgtcaat ccagtgattc gccccctggt cgtgataatc
    11341 accgcgccag cacctttttc tgctgtcgca ccgccagtaa accaggcgcg aacctgtttg
    11401 atttttggta gggtcatgat gttctccatt gttatgaggc ctgtaagtca aagggacttt
    11461 tccatcccaa cagacgtgaa atatccctgg cgcaggcaat ggccttgccc gccagataat
    11521 cacggtattc ttcattgatt tgtaagcggg taccgaccac cgagatcgca gcggtaagct
    11581 cgttattggc gttaaacacc ggcgcagcga cacaacggac atcggggtaa tcttcgccgt
    11641 tgtcatagct ccagccctga cggcgaatac gcgccagttc ttcgtgaagt tgctgtggat
    11701 gagtaatcgt tgtgggtgtc gcctgctccc agaccagccc ttcgataata ctttgctgta
    11761 ccgctgcagg ttgccaggca agcaggcatt tacctattcc ggagcgataa agcgaaaggc
    11821 tttttccttc atgggaacgc acgctgatag tggctgatga ctccactttt aaaatgtaat
    11881 aagcgctgcc gttatcaata atccccaggt gacataacag cccggtggta tccatcagtt
    11941 gcgtcaaacg cggtcgagcc agttcccgga gatccatctt gcttaacgca tgaccagaaa
    12001 gctccaccag cctggtccac aaacaaaaat tttcctggtg atcaaggcta agaaaacgct
    12061 gacgcctgag ttcattaagc agcaaatagg ccgtgctttt agggattccc aatgtgtcaa
    12121 tgatcgtcgc agcactacag gggccaatgc gggcaatcaa attgagaata tcaatagccc
    12181 gtgtcagggc gggaactttg cttgattcca acatactgga ctccagtctt agatactgga
    12241 atcagtctcg ttgctcaggg ggagtaaaat tgtgaagcgc atcaaatccc gctcctcgtc
    12301 cagcggactg gagaaattac gcacaaattt gtgaatcagt gcacggattt tttcttattg
    12361 agtacaacag gttggacaag ttttgccgga gggggaatgt gccggtgcag taaaccggca
    12421 cagagtggcg gaatcagaca gataaggtca tggactcaac aacctcaatc cagccatgct
    12481 cactggcgat atcctggccg tttaaccaac ggcgcagcat attcagtgcc atcattgcgc
    12541 acacttcctg acggatagcc aggctgtagc gtgtagtgct gaaacgcaca cgcagagcga
    12601 aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc gttctcgaaa cccgaaacag
    12661 ccagtgccag cccggcaaaa tggttggccc gccgttctgt aatccagtgc gcagtttgcg
    12721 ccagggtttc ttcctgtgaa ggaaccactt cacacgccag caatggagca cctgcgcgag
    12781 aaagttgcaa agccaataaa ccaccggtga attgctcgct caacgtcagg ctgaactggc
    12841 ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc
    12901 tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt tgctcgcttg
    12961 ccggtccggt gagtttcagt tcgatgatcg gcattgagga gcgatagccc attgttacgc
    13021 ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg
    13081 tagttaaacg cagacaaacc ggcgactgtg gtaaagaaaa acgctcgcgc aggcgcggca
    13141 ggatttcgtg ctcgaccatc accttaaatt ctgacggtac gccgggagta aagaacatca
    13201 ggcaacgatt aagccgcacg gcaaaaccac aggcggtgcc taccgggtta ttgataaatt
    13261 cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccacgtt
    13321 cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt
    13381 ttgctgtcgc agcggcgagt gcacttaaat catcactggt cggtcccagc ccgccgttaa
    13441 cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt
    13501 tatcccccac cgtattgcgg cgagataatg gcaacccctg atgaaagaaa aaatcggcca
    13561 gccaggcagc gttagtgtca acgatttgcc cgtgtaacac ttcatccccg gtggataaca
    13621 tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg
    13681 agataaggcg tagtaagaaa ctgaatggcg cgacagaatg ccgcgccgga agggatcaga
    13741 aactggcgtt aaccccaaca tacgggccgt cagccacggc gttgtcgcgg ttaccgtctt
    13801 tacccgacag attcaggtag cgataacccg cctcaatact gactggatgc ataatggtgt
    13861 aacgcgcgcc agcattcgct tcctcataac ttttaatacc gctggagagt gaatccggag
    13921 agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc
    13981 ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtaaacg ccttttccgc
    14041 caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg ccagcgacgt
    14101 caccgtcgtc gtcgttatgt gtccagttac cgcttaacgc caggcccgtc gattcagtcc
    14161 caaaaccgac accaatattg gtgtattctt tacccgcctg accgctgatg ctcattgcat
    14221 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    14281 tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc
    14341 acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    14401 aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa
    14461 gtgcagttat catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    14521 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc
    14581 cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga tcgcggcgtt
    14641 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag
    14701 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    14761 ccgtggacct tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag
    14821 atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac
    14881 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat
    14941 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    15001 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    15061 ttttccacat gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta
    15121 tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc
    15181 gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt
    15241 ttatctaccg tcagtttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg
    15301 gtattactgg aataaagatc gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca
    15361 cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt
    15421 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    15481 ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta
    15541 cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat
    15601 gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa ccagacaatt
    15661 accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    15721 tacgatgtcg gcaaaatgcc tcaaaatttt gccaaatgca aagcctaaat aagaaaaaat
    15781 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    15841 cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    15901 gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca
    15961 gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    16021 accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat
    16081 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    16141 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    16201 tccttgttgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    16261 cctgaagcga tcgagtcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    16321 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    16381 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    16441 aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc ggaaggtggc
    16501 ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt
    16561 ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta
    16621 acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    16681 gcaaaattag agcacctcaa cacccatcgg cgcgaaattg cccagcaata tcagcaagca
    16741 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    16801 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    16861 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    16921 aaatattatc gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa
    16981 cgcatctgtt ctttgccgct gttcccggat atgactaccg ccgatgccga ccgtgtcatc
    17041 acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    17101 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    17161 accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc
    17221 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt
    17281 gtgtctattt tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt
    17341 cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa
    17401 atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    17461 aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaacag gcttattcag
    17521 cgcaccaccg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat
    17581 attgtcgatg cgatgttgca ctgccatgaa cgtagcacct ttatcccgat tctggcgaat
    17641 atcttcgccc gccgtgccat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa
    17701 tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc
    17761 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc
    17821 attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc
    17881 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    17941 ttgctcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    18001 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    18061 cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta
    18121 gcgccatttt tacccatacc gataatcccg ctgaaaaagc cttttatggt tcggtggctc
    18181 gtctggcggc ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt
    18241 gggtggaacg cattgcccaa ctctcaccag aggtgatttt ctctttttat tatcgccatc
    18301 ttatttgcga cgagattttg cagctcgctc ccgcaggtgc atttaatctg cacggttcgc
    18361 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    18421 aaacgggcgt tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc
    18481 aactgcgcgt tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc
    18541 atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg
    18601 aaattgccca gcgcgaaaac gaagccacct gttttggtca cagaacgcca gatgacagtt
    18661 tccttgaatg gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc
    18721 cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg
    18781 ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga
    18841 ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta
    18901 tgcagggctc gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc
    18961 agcctgcctg caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta
    19021 ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg
    19081 atattggcag cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag
    19141 gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct
    19201 tgccgctggt ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg
    19261 aactcgattt tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa
    19321 tcatcttccc gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg
    19381 accattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa
    19441 aacaattact tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc
    19501 tcttccgccc gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg
    19561 gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc
    19621 taattgatgg cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt
    19681 tgtaccgcat tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca
    19741 atcctgagaa cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa
    19801 aacatccgct gcgccatcac ttcccaccgt ttgcgggctt tcgtgttgtc gaaagtagca
    19861 gctactacgg caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc
    19921 gccgctgcct ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg
    19981 atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg
    20041 cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccacgtctgc tggaaatctt
    20101 gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg
    20161 ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc
    20221 atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg
    20281 tcatgccaat gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc
    20341 ctgggatcac catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga
    20401 cgatattgca cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc
    20461 tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt
    20521 gcgctacaac agcgattgtc gtggggccat gccgttccgt ccactgctcg aatcaggaaa
    20581 ccctggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga
    20641 tgtgaaagca gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac
    20701 gccggtttat accattcatg cagaagttga aggctgcgct tatcagcata attttgtgga
    20761 tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc
    20821 agagacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct
    20881 gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcctc
    20941 ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc
    21001 gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg
    21061 ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc
    21121 attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg
    21181 accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt
    21241 ctggctctac tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact
    21301 tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg
    21361 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    21421 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    21481 gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    21541 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    21601 cctgacttct ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat
    21661 gcccaacata gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg
    21721 tggctgggat tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca
    21781 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggt
    21841 aaattgccca cctatattct ttcctgcttt gcatctctgg caatgctgat ggcgcattac
    21901 gctttgctgg cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc
    21961 gcttttggct tcactggcat tattgccaca tttgtggtct ccccgtgggg accaatgaac
    22021 acgccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg
    22081 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtcttttgcc
    22141 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    22201 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    22261 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    22321 gacatcatca tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg
    22381 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    22441 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt
    22501 ccgcccgcag atgccatcga tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa
    22561 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    22621 aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg
    22681 gactggcgct ggcttgtctt ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg
    22741 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg
    22801 cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta
    22861 ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag
    22921 cgtcattatt gcctcagttg cgcaattaag cctgggtttt gcggcgagtc atctgccgcc
    22981 gatgacgcac ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat
    23041 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca
    23101 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    23161 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    23221 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg
    23281 agttttccct gccactttac actgccgttc ccactcatcg gggctataac tgcttgccga
    23341 gaggactttc accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg
    23401 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    23461 cgcgccgcca gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga
    23521 ttttttgacc agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta
    23581 cagattaaca gataaaccta ttatcaacgg caatggaatg gcaaaattgc gaagtgaaac
    23641 taatgatttc gtaatcctgg cgttgcaccc actcttttaa cgcctgacgt gaaattttaa
    23701 taccgccgtt tttcagctcc ggcggtaaag ttagccagcg caccggttgt tgaaaacgag
    23761 ccagcttatc tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg
    23821 ctaccggtcg atgaccaaac tccttgtcgg caacggggac gataaacacc tgcaaaaccg
    23881 caggatgtgc agcaattacg cgctcgactt cttccggctg aataccctct ccgccactga
    23941 agaacagatt gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat
    24001 cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt
    24061 aaccttctgc catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc
    24121 gacccggcag cggcgaacca acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg
    24181 aggcaaactc ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt
    24241 ccgtcaactc gaccgggata gccgcgccgc caagtaacac cgctttcagg gaaacggaac
    24301 tgcggttaac cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg
    24361 ccagcatttg ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc
    24421 agcgccacat aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat
    24481 cgtgatcgcc aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat
    24541 aagtatggac agcggctttc ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca
    24601 gacgcgtcgg ctgccacgta gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg
    24661 ttaatgccgg aaacgtgttt tccccatccg gcaccagcgc aaattgcagc gtcagattgg
    24721 gcagcaattc ttcaagcaac ggttgcggca gctgagggtt cacgggcaac acccgcgccc
    24781 cgcattgcag taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca
    24841 tcacgccgct gccctcaacc acaccctgcg ccgcaaagcc cgtagctaac tcatcgacgc
    24901 gagcacaaag ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc
    24961 cccgcacttg ccgccagtga cgccacggcc agtcagagaa gatcataaca actgctccag
    25021 tacatcagcg tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg
    25081 catcagatcc agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg
    25141 cgccagttgc gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc
    25201 cagcgcatgc gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg
    25261 tttgataact accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag
    25321 gctttcatcc caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg
    25381 ggttttgcac ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt
    25441 ggcaaactgc tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag
    25501 atccggaatt gcctccagca acagattaac caccatgccg tcgcgcacgg cttcgtacaa
    25561 tcccactttg accttcgcca ctttctcgcc tggcatatca gcaagtttga ggatcagatc
    25621 gtcaggatcg ccattacaca gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc
    25681 tgccagctcc gccaatgcac agcttacgcc aaaggcaacg gaaggcatct gcggtagctc
    25741 gccatcgcct gccagccagt tatttaccca agcaagcagc acaatttgcg cctcttccca
    25801 ggtttcctga ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccttc
    25861 acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccatccc
    25921 cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta
    25981 aatttgctga aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct
    26041 tccgtcatgt agaacagcat ggtggcgttg cccgccagct cctgcagacc cgcctgaccg
    26101 tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc
    26161 atttcgcggc accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg
    26221 acaaggccca tatccagcgc ctgttttgca tcgtactgac ggcacaggaa ccagatttca
    26281 cgcgcttttt tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag
    26341 gaaccgactt tcgggccagt ctgaccgaag atggcattat ctgccgcgat agtcaggtcg
    26401 cacatcatgt gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc
    26461 ggtttcggac aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg
    26521 gaatcatctt tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac
    26581 gctttatcgc ctgcgccagt cagaatgatc acgccgacgt tgtcgtcata acgcgcatct
    26641 gccagtgcct ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga
    26701 cgattaatgg tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa
    26761 ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc
    26821 atattgtgtc ctttagtcag aaacgcaaga tctgcgccag acttgcgata acgccagcgg
    26881 gattttcccg atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt
    26941 ccgccgccaa ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg
    27001 tgcgggcgct aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca
    27061 tggcggcaag ggttgcgcca ttattgttac tgcgcagcgc caccagctcc cggcgttgat
    27121 cgtcattgag cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg
    27181 gttctgtgcg aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt
    27241 cagcattttg caacccagga tgcccgcctt cgacaacaac cccacaaagc cccggcatcc
    27301 cctggcaagc cgccatcatc gccacccgtc ccccaagcga gtaccccacc aaccagaagt
    27361 taaggatgtt gtaactaacc agggttttac gcagtaagtc ggtgacatca tcaaatccat
    27421 cgacgctaat cgccgccgaa ccaccgtgac ctgggagata aacatacaac cgtgagtagt
    27481 cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa
    27541 acaccagcca gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg
    27601 gcttacctgc gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat
    27661 ttcaattacc gtggtggttg gcgtgcgcca ggcatcggca agtgccgttt caagttcctg
    27721 ccagttttgc ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac
    27781 gttttgcggc atcagataga aacgctcgcg ctcgcttttc ggcgttggca gcagcgagaa
    27841 aatttgcccg ccgttgttgt tcaccacaat taatatcagc ggcgcggaaa cctgacgcaa
    27901 taacgccagc gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg
    27961 tttgccgctt gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc
    28021 cccacggttg ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg
    28081 taccaccagg ctgttaccga caaacaattg cccctgttca ggcagatagt cgctgatgcg
    28141 atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg
    28201 ttccgccagg cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc
    28261 cagccagtcg gcaatattgg caattaagcg gcgtccacga tggtgcgccg gatcaagccg
    28321 cccttcgatg tcatccacaa tccagtactc ttcgggttca cagcttgcct gccattgcag
    28381 gagccgtttg cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gttgcagttc
    28441 gctggtcgct ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg
    28501 cgacagcacg tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt
    28561 gccctcttcc gcactcatgc gcccggcaac caccacgccg cgtttttgtc gccagaagaa
    28621 ccagtcgcgc tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc
    28681 ctgccaccag tcgcccagcc gctgttgcca gctaagcccg gtgtcgtcca tttcgccata
    28741 cagcggttcg gcaaacgggc agttgatatg gactccacca gaatgaagcg taccgagagc
    28801 atggtcgatg gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga
    28861 aatactgtgc gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc
    28921 gcagtcaatc agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa
    28981 cccagcttca atcagtgccg gatagagatt tgctaccgcc gtaccggagg tcacaatcac
    29041 cgccaccggc tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc
    29101 atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt
    29161 agaacgcgag ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag
    29221 aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa
    29281 tattgcggct aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg
    29341 cgactcatta ctattccatc tgtaataaag tacgcagccc tgccgctttg ttgtcgattt
    29401 cctgccactc ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca
    29461 cgacattgcc gctaattttt gctgagcgca gggaaacgca gaattcgctt tgctgtaatg
    29521 agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa
    29581 actgtcgcgc cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta
    29641 aacagatcac attatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca
    29701 ctttacgcag acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta
    29761 accgttggca gatatcttcc accaccagca tattctcgcg ctggttttta tcatccgcca
    29821 tcagccactc acctaactgc tgcgcctgct tatcatcagg gtgatttgct accgttcccg
    29881 ccagcgcttc agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc
    29941 caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc
    30001 gacgactggc agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg
    30061 cccgagcgag caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga
    30121 ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta
    30181 acccaggcaa gggcttgata ctcaccagtg tggcgataaa ttctcttgcc ttaatcgcat
    30241 cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc
    30301 gtcgccattc caggcgtggt aaaagtaaat tgccctgcga cgggtcaaac gcattcagcc
    30361 cccaaatgcg taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca
    30421 acgacgtaaa acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt
    30481 gccagtagaa ttgcgggtat gtttgctgac tggccagcca gctcaaggca tcaaaagcgt
    30541 ctttgagagg gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg
    30601 acaaatggcg cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct
    30661 gttaaaagcc tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga
    30721 ggttttaacg gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa
    30781 ttccttgcca gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc
    30841 gatagtaata actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt
    30901 ctgcacgcgc tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca
    30961 cctcttccag tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact
    31021 gattagacat tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg
    31081 ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat
    31141 agacctccgt cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg
    31201 ccccgaggta aacaggctta tcaggccagt gatgcgtgca ggtgtcgagg gttttactca
    31261 tcagttgctg ccccactttt tcgccacgca acgcttcact gacaatcacc cgaccaataa
    31321 cgaccggctc aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat
    31381 ttttccaacc gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat
    31441 aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct
    31501 gggaaaccga taattcagag tgatgcagat cttaccattc aatcataatg ctctcctttt
    31561 agatgtatgc cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa
    31621 tttttttagg tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc
    31681 ttaacctgca acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc
    31741 agcatcagct actgcatacc gcctttaacc ctgggaaact ggacaagatt tttatcagtc
    31801 accttcatgg cgatcatctc tttggtttac ccggcttgct gtgcagtcgt tctatgtcag
    31861 gcattatcca acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg
    31921 cgctgcggat tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg
    31981 gcgaaattct cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg
    32041 aatgttatgg ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat
    32101 taaaagctgc tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa
    32161 tcacgctgga agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag
    32221 gtaaagcgct cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta
    32281 aaggtgtcga tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca
    32341 atagtcgcgg ccatagctct acacgccagg ctgcgacact agcccgtgag gctggagtcg
    32401 gcaagctaat cattacccac gtcagctcgc gctatgatga caaaggttgt caacacctgt
    32461 tacgtgaatg caggtcaatt ttcccggcga ctgaactggc gaatgatttc accgtgttta
    32521 acgtttaact
//
LOCUS       DAJVYM010000034        29940 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVYM010000034 DAJVYM010000000
VERSION     DAJVYM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.034"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQS60_001456"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQS60_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS2387244.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQS60_001457"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQS60_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS2387245.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OQS60_001458"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OQS60_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS2387246.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OQS60_001459"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OQS60_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS2387247.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OQS60_001460"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OQS60_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS2387248.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OQS60_001461"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OQS60_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS2387249.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OQS60_001462"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OQS60_001462"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS2387250.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OQS60_001463"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OQS60_001463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS2387251.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQS60_001464"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQS60_001464"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS2387252.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQS60_001465"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQS60_001465"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS2387253.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQS60_001466"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQS60_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS2387254.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQS60_001467"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQS60_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS2387255.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQS60_001468"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQS60_001468"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS2387256.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQS60_001469"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQS60_001469"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS2387257.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQS60_001470"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQS60_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS2387258.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OQS60_001471"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OQS60_001471"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS2387259.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OQS60_001472"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OQS60_001472"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS2387260.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20761..21432
                     /gene="nfi"
                     /locus_tag="OQS60_001473"
     CDS             20761..21432
                     /gene="nfi"
                     /locus_tag="OQS60_001473"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS2387261.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21475..22065
                     /locus_tag="OQS60_001474"
     CDS             21475..22065
                     /locus_tag="OQS60_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS2387262.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22252..22524
                     /gene="hupA"
                     /locus_tag="OQS60_001475"
     CDS             22252..22524
                     /gene="hupA"
                     /locus_tag="OQS60_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS2387263.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22537..23232
                     /locus_tag="OQS60_001476"
     CDS             22537..23232
                     /locus_tag="OQS60_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS2387264.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OQS60_001477"
     CDS             complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OQS60_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS2387265.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            23897..25273
                     /gene="zraS"
                     /locus_tag="OQS60_001478"
     CDS             23897..25273
                     /gene="zraS"
                     /locus_tag="OQS60_001478"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS2387266.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25270..26595
                     /gene="zraR"
                     /locus_tag="OQS60_001479"
     CDS             25270..26595
                     /gene="zraR"
                     /locus_tag="OQS60_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS2387267.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OQS60_001480"
     CDS             complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OQS60_001480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS2387268.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OQS60_001481"
     CDS             complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OQS60_001481"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS2387269.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7014 a         7804 c         8246 g         6876 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgcta cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacata
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgcttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gtaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaatccc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgactttttg gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggagctggtg
     4861 ccggaacgcc tgcgtggtga aactgcatct ttcgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgttg
     5101 gctaagctga gccagtctgg tcacaagcgt attgaaacgc tgttcaccaa cgacctggat
     5161 cacggcccgt atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acctgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttctg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggacatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccgg gtggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcattacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctttgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaggtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tttctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgtgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtttgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 accgacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggtg tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cactggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag tggttccaga
    13681 tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gttctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccag
    13861 tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcta
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagggcc tgcgcggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcatttcggc ggctttcaga gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc
    14881 gaacagtcgg agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtaccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga cctgcgattt
    15301 cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cccgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtccaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat
    15721 gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca ttgcgccgat ctttgatgcg tagctggagg gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat
    16201 ttctccgcct ctggcgcgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcacgta cttcctggct gattttcatc gagcagaatt tcggcccaca
    16321 catggagcaa aaatgggcga ctttacctga ctcttgcggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcctgga tgccctttcg ccaggtcagc
    16501 ggcgtggacg gcaatcttat aggtgataag cccctgctta acatcttctt tattaggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca
    16801 ggcaattttg gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagtcaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt agtacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgaggcgt acaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcgcgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg cgacctacat ggtggaaggc
    20161 ggcagcagca aagcgttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctac tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gtgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcg
    20461 atggcagaaa ccggttgcga tgcgttgggc ctcgactgga caacggatat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacaat atcaccgcta aggagtgatt
    20761 atggatctcg cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatccgcgag
    20821 gatcgactcg ataaagatcc accggatctg atcgccggag tcgatgtcgg gtttgagcag
    20881 ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtg
    20941 gagtataaag ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc
    21001 cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg
    21061 tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg gcgtcgctag ccattttggt
    21121 ttgatggtgg atgtgccaac gatcggcgtg gcgaaaaaac ggctctgcgg taaattcgaa
    21181 ccgctctcca gcgaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc
    21241 tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc
    21301 agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag
    21361 ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca
    21421 aatcagccct aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta
    21481 caaaacccaa ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg
    21541 gcttgcttat gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt
    21601 ggtgatgggc aaatttaccg tcgtattctc gatctcatct gggaaacgct gaccgttaaa
    21661 gacgcaaaag taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc
    21721 gacgatttcg atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa
    21781 ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc
    21841 tccatcacga ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gagcgatgaa
    21901 gagcttaaag aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga
    21961 cttttagctg agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg
    22021 cgtgaggcgg gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga
    22081 tttaacgcct gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt
    22141 tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt
    22201 tccgtcgcac tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag
    22261 actcaactga ttgatgtaat tgcagagaaa gcagaactgt ccaaaactca ggctaaagct
    22321 gctctggagt ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa
    22381 ctggttggtt tcggtacctt caaagtgaac caccgcgctg agcgtactgg ccgcaacccg
    22441 cagaccggta aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag
    22501 gcactgaaag acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg
    22561 gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc tggctggcgc tctgcttctc
    22621 actgcctgta gtcataactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac
    22681 cagggcgcgg tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg
    22741 tttagcccgt ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac
    22801 ctcacgctca tcaatatcaa tgtttacagc aaaccgccgg tgaatattcg cgcgcgtttt
    22861 gacgatcgcg gtgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt
    22921 tctaacgacc aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat
    22981 gccttacgtc aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc
    23041 gtgaccacct gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat
    23101 atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc
    23161 gaaggttcgc aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag
    23221 aaaacgttct gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt
    23281 agcccatgcc cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt
    23341 tcacccgtaa ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa
    23401 ttttgctgct atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca
    23461 ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg
    23521 ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt
    23581 gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa
    23641 ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc
    23701 ttccgtgcca acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa
    23761 atgactcgcc tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt
    23821 tacagcgcag ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga
    23881 gcaggcaaag aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt
    23941 aagcgcgatc ctccccgtgg tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat
    24001 tcgcgattat gggcgcgaga ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt
    24061 acttattcgc gctcttgaat ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc
    24121 gcagcagcag gccttactgg aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt
    24181 cacggatgaa caaggaacaa tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct
    24241 ttattccccg caggaaatgc agcagttaca tccgggagat gaagaagcgt ggcggcggat
    24301 cgatagcgca gacggcgagc ctgttctgga aatttatcgc cagtttcaac cgatgtttgc
    24361 tgctggaatg caccggatgc gccatatgca gcagtatgcc gcgacaccac aagcaatttt
    24421 catcgctttc gacgccagta atattgtgag tgccgaagat cgtgagcaga gaaacaccct
    24481 gattatcctc ttcgccctgg cgacggtctt gctggcaagc gtattgtcat tcttctggta
    24541 tcgccgctat ttgcgctcgc gccagcttct acaagatgaa atgaagcgca aagagaagct
    24601 ggtggcgctg gggcatcttg cggcaggcgt tgcccacgaa atccgtaacc cactttcctc
    24661 gattaaagga ctggcgaaat actttgccga gcgcgcgcct gcagggggag aagcgcatca
    24721 actggcgcag gtgatggcga aagaggccga ccgtttaaac cgcgtggtaa gcgagctgct
    24781 ggaactggtt aagccaacgc atctggcttt gcaggcggtg gatctcaaca cgctgattaa
    24841 ccactcatta cagctggtaa gccaggatgc gaacagccgg gagatccagt tacgctttac
    24901 cgccaacgac acattaccgg aaattcaggc cgatccggac aggctgactc aggtcctgtt
    24961 gaatctctat ctcaatgcta ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc
    25021 cagcgaaagc ggcgcgggcg tgaaaatcag cgttaccgac agcggtaagg gaattgcggc
    25081 agatcagctt gatgccatct tcactccgta cttcaccact aaagccgaag gcaccggact
    25141 ggggctggcg gtcgtgcata atattgttga acaacacggt ggtacaattc aggtcgcaag
    25201 ccaggaggga aaaggcgcaa cgttcaccct ctggcttccg gtcactatta cgcgtaaggg
    25261 cccacaagga tgacgcacga taatatcgat attctggtgg tggatgatga cattagccac
    25321 tgcactattt tgcaggcttt actgcgcggc tggggctata acgtcgcgct ggcgaacagc
    25381 gggcgacagg cgctggagca ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg
    25441 cgaatggcgg agatggacgg cattgccacg ctaaaagaga tcaaagcgtt aaatccggcg
    25501 attccggtgc tgattatgac cgcatactcc agcgtcgaga cggcggtaga agcgttgaaa
    25561 accggggcgc tggattatct catcaagccg ctggatttcg ataacctgca ggcgacgctg
    25621 gaaaaggcgc tcgcgcatac gcacagtatt gacgctgaaa cgcctgcggt gtctgccagc
    25681 cagttcggta tggtcggtaa aagcccggcg atgcaacacc tgctcagtga aatcgccctc
    25741 gtcgcgccat cggaagccac ggtgctgatc cacggcgatt ccggcaccgg taaagagctg
    25801 gtcgccaggg cgattcacgc cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac
    25861 tgtgcggcac tcaacgaatc cttgctggaa tctgaattgt tcggtcacga aaaaggggcg
    25921 ttcactggag ccgatagacg ccgggaaggg cgctttgttg aggcggacgg cggcacgctg
    25981 tttctcgatg aaattggcga tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt
    26041 caggagcgcg aagttcagcg tgtcggtagc aaccagacta tctcggttga tgtccggttg
    26101 attgcggcga cccatcgcga tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat
    26161 ctctactatc gcctgaatgt ggtggcgatt gaagtaccat cgctgcggca acggcgggaa
    26221 gatatccctc tgctggctgt ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg
    26281 gtaaaaggct ttacgccaca ggcgatggat ttgctgatcc actacgactg gccgggaaat
    26341 attcgtgaac tggaaaacgc ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt
    26401 tccgaacgcg agctgccgct ggcgattgcc ggtacgccga tcccactggg gcaaagtcag
    26461 gatattcagc cgctggtgga agtcgaaaaa gaagtgattc tggcggcact ggagaaaacg
    26521 ggcggcaaca aaaccgaagc cgcccgtcag ttagggatca cgcgcaaaac gctattggca
    26581 aaactgtcgc gttagttctg ctcgcgttcg atagcgcgcc agccgatatc tttccggcag
    26641 aagcagtcgt cccagtgaat atcggtcatt aaggcataag cgcgtttctg cgcttctgcc
    26701 acggtatgac ccagcgcggt gacgcacagt acgcgcccgc cgctggtcac tacctgctcg
    26761 ttatccgcca gtttcgtgcc cgcgtggaac actttgccgc ctgccacttc ttccagcggc
    26821 aggccgtgga tcacatcgcc ggtgcggtaa tcgcccggat atccacccgc agccatcacc
    26881 acgccgagag aagcacgctc atcccactct gacgttttct cgtccagttt gccttcacag
    26941 gccgccaggc agagctcaac cagatcggac ttcatgcgca gcataatcgg ctgggtttct
    27001 ggatcgccaa agcggcagtt aaattcgata accttcggat tgccctgttt gtcgatcatc
    27061 aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt
    27121 ggccagatga tacgttccat ggtgcgctga tgaacttcat cggttactac cggcgcgggg
    27181 gagtaagcgc ccatcccgcc cgtgtttggt ccggtatctt tatcgcctac gcgtttgtga
    27241 tcctggctgg tggccatcgg cagcacatgc tcgccgtcta ccatcacgat aaagctcgct
    27301 tcttcgccgt cgaggaactc ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg
    27361 cccgccagca tatcgtgaac agccgcttcg gcttcttcca gcgtcatcgc cacgataacg
    27421 cctttcccgg ctgccagacc gtccgcttta atgacgatcg gcgcaccttt ctcacgcaga
    27481 tacgccagcg caggttctac ctcggtgaag ttctggtatt ccgctgtagg gatcttatgg
    27541 cgggccagga aatctttggt aaacgctttc gaaccttcca gttgcgcggc acctgcggtt
    27601 gggccgaaga ttttcatccc ggcggcgcgg aaggtatcga ccacgccttt caccagcggc
    27661 gcttccgggc cgacgatggt cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc
    27721 gggatatcgg tcacgccgat agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca
    27781 ttacccggag caacaaaaac agtttcaacc agcggcgact gcgccgcttt ccaggccagc
    27841 gcgtgctcgc gcccgccgtt accaatcact aatactttca tctattgctc cattaatggc
    27901 ggaagtggcg catgtcggtg aagagcatcg caataccgtg ctcgtcggcg gcggcaatca
    27961 cttcgtcatc acggatagaa ccgccaggct ggattacgca ggtcacgcct gcagcggcgg
    28021 cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga agccatcgag gaacctttca
    28081 cttccaagcc ttcatcggca gctttaatac cggcgatttt cgcggagtac acgcggctca
    28141 tctggcccgc gccaatcccg atggtcatat tgtttttggc atagacgata gcgttggatt
    28201 tcacgaactt cgccactttc cagcagaaca gcgcatcacg cagttcctgt tcggtcggct
    28261 gacgttgggt gacgacgcgc agttcttccg caccgaccat gcccaggtca cgatcctgaa
    28321 ccagcagacc gccgttcacg cgtttgaaat caagacccgg aacacgctcg ccccactgac
    28381 cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat tttcagggct tcttcgctgg
    28441 cggaaggcgc aataatcact tcgacaaact gacgagaaat gatggcctgt gcggtttccg
    28501 catccagctc gcggttaaag gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt
    28561 acgcgcgatc gtaagcatca agaatggaat tgccgatagc cacgccgcaa gggttggcgt
    28621 gcttcacaat cacacatgcc ggctcggcga actctttcac gcactccagc gccgcatcgg
    28681 tatcggcgat gttgttataa gagagggctt taccctgaac ctgggttgcg gtagcaacgg
    28741 aggcttcttt cacattctct tctatataga aggcagcctg ctggtggctg ttctcgccgt
    28801 aacgcatatc ctgcttctta atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg
    28861 cttctttgct ttcaccgtgg taagccggaa ccatgctgcc gaagtagttg gcaatcatgc
    28921 tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg
    28981 tcagcgatcc ttcgttgtca tccatctctt taataatggc gtcgtagtcg ctgctcttca
    29041 ccacgattgc gacatcttta tggttcttag cggcggagcg caccatggtc gggccgccga
    29101 tatcgatgtt ctcaaccgca tcttccagcg agcagccttc acgagcaaca gtctgggcga
    29161 acggatacag gttaacaacc accatatcga taggctggat ctgatgttct tccataatgg
    29221 catcgtcctg gccgcgacgg cccagaatgc caccatgtac tttcggatgc agggtcttca
    29281 cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga aacttcggtt accggcagac
    29341 ctttttctgc taacagacgg gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg
    29401 aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga aacactgagc agagcgcggc
    29461 ggactggacg acgttgttgc atggtaaatc ccctggattt gactattaca gagagcgtta
    29521 gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg gggcatcctt atttttcgcc
    29581 cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt ttctctttca atggtgatca
    29641 caattttgac agtggttacc gtgggaaaaa tacagaaatt acattgatga ttgtggataa
    29701 ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg aatgcagcag tcagtcattt
    29761 ttctgcaatt tttctattgc ggcctgcgga gaaatcccta taatgcgcct ccatcgacac
    29821 ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa atcctgaaat
    29881 tcagggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJVYM010000035        29548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVYM010000035 DAJVYM010000000
VERSION     DAJVYM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.035"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="OQS60_001482"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="OQS60_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCS2387270.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="OQS60_001483"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="OQS60_001483"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS2387271.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="OQS60_001484"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="OQS60_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS2387272.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="OQS60_001485"
     CDS             complement(3644..4030)
                     /locus_tag="OQS60_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS2387273.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OQS60_001486"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OQS60_001486"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS2387274.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OQS60_001487"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OQS60_001487"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS2387275.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OQS60_001488"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OQS60_001488"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS2387276.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="OQS60_001489"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="OQS60_001489"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS2387277.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="OQS60_001490"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="OQS60_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS2387278.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="OQS60_001491"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="OQS60_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS2387279.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="OQS60_001492"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="OQS60_001492"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS2387280.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="OQS60_001493"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="OQS60_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS2387281.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="OQS60_001494"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="OQS60_001494"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS2387282.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="OQS60_001495"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="OQS60_001495"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS2387283.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="OQS60_001496"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="OQS60_001496"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS2387284.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="OQS60_001497"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="OQS60_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS2387285.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="OQS60_001498"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="OQS60_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS2387286.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="OQS60_001499"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="OQS60_001499"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS2387287.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="OQS60_001500"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="OQS60_001500"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS2387288.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="OQS60_001501"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="OQS60_001501"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS2387289.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="OQS60_001502"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="OQS60_001502"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS2387290.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="OQS60_001503"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="OQS60_001503"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS2387291.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="OQS60_001504"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="OQS60_001504"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS2387292.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="OQS60_001505"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="OQS60_001505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS2387293.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="OQS60_001506"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="OQS60_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS2387294.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAJVYM010000036        28797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVYM010000036 DAJVYM010000000
VERSION     DAJVYM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.036"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQS60_001507"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQS60_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS2387295.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OQS60_001508"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OQS60_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS2387296.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OQS60_001509"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OQS60_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS2387297.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OQS60_001510"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OQS60_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS2387298.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OQS60_001511"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OQS60_001511"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS2387299.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OQS60_001512"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OQS60_001512"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS2387300.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OQS60_001513"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OQS60_001513"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS2387301.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OQS60_001514"
     CDS             complement(4604..5326)
                     /locus_tag="OQS60_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2387302.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OQS60_001515"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OQS60_001515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS2387303.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OQS60_001516"
     CDS             6526..6744
                     /locus_tag="OQS60_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS2387304.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OQS60_001517"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OQS60_001517"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS2387305.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OQS60_001518"
     CDS             complement(7478..7678)
                     /locus_tag="OQS60_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS2387306.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OQS60_001519"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OQS60_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS2387307.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQS60_001520"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQS60_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS2387308.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OQS60_001521"
     CDS             10175..12088
                     /locus_tag="OQS60_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2387309.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OQS60_001522"
     CDS             12088..13110
                     /locus_tag="OQS60_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2387310.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OQS60_001523"
     CDS             13104..13322
                     /locus_tag="OQS60_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS2387311.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OQS60_001524"
     CDS             13376..14245
                     /locus_tag="OQS60_001524"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS2387312.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OQS60_001525"
     CDS             complement(14300..14704)
                     /locus_tag="OQS60_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS2387313.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OQS60_001526"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OQS60_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS2387314.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OQS60_001527"
     CDS             15689..17779
                     /locus_tag="OQS60_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS2387315.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OQS60_001528"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OQS60_001528"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS2387316.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OQS60_001529"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OQS60_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS2387317.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OQS60_001530"
     CDS             complement(19747..20349)
                     /locus_tag="OQS60_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS2387318.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OQS60_001531"
     CDS             complement(20339..20506)
                     /locus_tag="OQS60_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS2387319.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OQS60_001532"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OQS60_001532"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS2387320.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OQS60_001533"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OQS60_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS2387321.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OQS60_001534"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OQS60_001534"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS2387322.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OQS60_001535"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OQS60_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS2387323.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OQS60_001536"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OQS60_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS2387324.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OQS60_001537"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OQS60_001537"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS2387325.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OQS60_001538"
     CDS             28334..28501
                     /locus_tag="OQS60_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS2387326.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6996 a         7710 c         7218 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcaa tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAJVYM010000037        28608 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVYM010000037 DAJVYM010000000
VERSION     DAJVYM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.037"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OQS60_001539"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OQS60_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS2387327.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="OQS60_001540"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="OQS60_001540"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS2387328.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="OQS60_001541"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="OQS60_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS2387329.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="OQS60_001542"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="OQS60_001542"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS2387330.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="OQS60_001543"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="OQS60_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS2387331.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="OQS60_001544"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="OQS60_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS2387332.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="OQS60_001545"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="OQS60_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS2387333.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="OQS60_001546"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="OQS60_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS2387334.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="OQS60_001547"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="OQS60_001547"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS2387335.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OQS60_001548"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OQS60_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS2387336.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OQS60_001549"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OQS60_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS2387337.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="OQS60_001550"
     CDS             complement(13980..14846)
                     /locus_tag="OQS60_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS2387338.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OQS60_001551"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OQS60_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS2387339.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="OQS60_001552"
     CDS             17484..17819
                     /locus_tag="OQS60_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS2387340.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="OQS60_001553"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="OQS60_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="OQS60_001554"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="OQS60_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS2387341.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="OQS60_001555"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="OQS60_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2387342.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="OQS60_001556"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="OQS60_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS2387343.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="OQS60_001557"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="OQS60_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS2387344.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="OQS60_001558"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="OQS60_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS2387345.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="OQS60_001559"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="OQS60_001559"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS2387346.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="OQS60_001560"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="OQS60_001560"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS2387347.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="OQS60_001561"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="OQS60_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS2387348.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="OQS60_001562"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="OQS60_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS2387349.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="OQS60_001563"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="OQS60_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS2387350.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="OQS60_001564"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="OQS60_001564"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS2387351.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="OQS60_001565"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="OQS60_001565"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS2387352.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..>28608
                     /gene="phnO"
                     /locus_tag="OQS60_001566"
     CDS             28192..>28608
                     /gene="phnO"
                     /locus_tag="OQS60_001566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS2387353.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFR"
BASE COUNT         6926 a         7936 c         7384 g         6362 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgc
//
LOCUS       DAJVYM010000038        28041 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVYM010000038 DAJVYM010000000
VERSION     DAJVYM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.038"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..133)
                     /locus_tag="OQS60_001567"
     CDS             complement(<1..133)
                     /locus_tag="OQS60_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2387354.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="OQS60_001568"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="OQS60_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(703..2026)
                     /locus_tag="OQS60_001569"
                     /pseudo
     CDS             complement(703..2026)
                     /locus_tag="OQS60_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2026..3642)
                     /locus_tag="OQS60_001570"
     CDS             complement(2026..3642)
                     /locus_tag="OQS60_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS2387355.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3938..4654
                     /locus_tag="OQS60_001571"
     CDS             3938..4654
                     /locus_tag="OQS60_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS2387356.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4651..6312)
                     /locus_tag="OQS60_001572"
     CDS             complement(4651..6312)
                     /locus_tag="OQS60_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS2387357.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="OQS60_001573"
     CDS             complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="OQS60_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS2387358.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="OQS60_001574"
     CDS             complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="OQS60_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS2387359.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7455..7527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7767..8099
                     /locus_tag="OQS60_001575"
     CDS             7767..8099
                     /locus_tag="OQS60_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS2387360.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8101..9315)
                     /locus_tag="OQS60_001576"
     CDS             complement(8101..9315)
                     /locus_tag="OQS60_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS2387361.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9416..10480
                     /gene="cbrA"
                     /locus_tag="OQS60_001577"
     CDS             9416..10480
                     /gene="cbrA"
                     /locus_tag="OQS60_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS2387362.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10477..11814)
                     /locus_tag="OQS60_001578"
     CDS             complement(10477..11814)
                     /locus_tag="OQS60_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2387363.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="OQS60_001579"
                     /pseudo
     CDS             complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="OQS60_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="OQS60_001580"
     CDS             complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="OQS60_001580"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS2387364.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="OQS60_001581"
     CDS             complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="OQS60_001581"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS2387365.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="OQS60_001582"
     CDS             complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="OQS60_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS2387366.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15168..15800
                     /locus_tag="OQS60_001583"
                     /pseudo
     CDS             15168..15800
                     /locus_tag="OQS60_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="OQS60_001584"
     CDS             complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="OQS60_001584"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS2387367.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16773..17171)
                     /locus_tag="OQS60_001585"
     CDS             complement(16773..17171)
                     /locus_tag="OQS60_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS2387368.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="OQS60_001586"
     CDS             complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="OQS60_001586"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS2387369.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19854..20927)
                     /gene="recF"
                     /locus_tag="OQS60_001587"
     CDS             complement(19854..20927)
                     /gene="recF"
                     /locus_tag="OQS60_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS2387370.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="OQS60_001588"
     CDS             complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="OQS60_001588"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS2387371.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="OQS60_001589"
     CDS             complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="OQS60_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS2387372.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23732..23812
                     /locus_tag="OQS60_001590"
     CDS             23732..23812
                     /locus_tag="OQS60_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387373.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24042..24182
                     /gene="rpmH"
                     /locus_tag="OQS60_001591"
     CDS             24042..24182
                     /gene="rpmH"
                     /locus_tag="OQS60_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS2387374.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24199..24558
                     /gene="rnpA"
                     /locus_tag="OQS60_001592"
     CDS             24199..24558
                     /gene="rnpA"
                     /locus_tag="OQS60_001592"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS2387375.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24522..24779
                     /gene="yidD"
                     /locus_tag="OQS60_001593"
     CDS             24522..24779
                     /gene="yidD"
                     /locus_tag="OQS60_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS2387376.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24782..26428
                     /gene="yidC"
                     /locus_tag="OQS60_001594"
     CDS             24782..26428
                     /gene="yidC"
                     /locus_tag="OQS60_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS2387377.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26534..27898
                     /gene="mnmE"
                     /locus_tag="OQS60_001595"
     CDS             26534..27898
                     /gene="mnmE"
                     /locus_tag="OQS60_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS2387378.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6749 a         7582 c         7146 g         6564 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct
      661 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
      721 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
      781 taccgttttc gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
      841 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
      901 gatatcgccg accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac
      961 catcgcttca tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg
     1021 ctcctgagtg ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc
     1081 aatctccagc ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa
     1141 aacctgtttc tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat
     1201 gctgaacaac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
     1261 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
     1321 gtgtatcttg ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca
     1381 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
     1441 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
     1501 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
     1561 tggcttctgc gacaatggct gccgggttgg agtagttgag catccaggca tttggtgaat
     1621 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
     1681 ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat
     1741 ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
     1801 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa
     1861 ggatgacttt acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt
     1921 tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg
     1981 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
     2041 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
     2101 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
     2161 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
     2221 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
     2281 caggataccg gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
     2341 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
     2401 gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
     2461 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
     2521 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
     2581 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
     2641 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
     2701 tgcgggaatc agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
     2761 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc
     2821 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
     2881 ccagtacatc tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat
     2941 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc
     3001 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg
     3061 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
     3121 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
     3181 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
     3241 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
     3301 gccgaagtag cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa
     3361 actcaccatc accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa
     3421 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
     3481 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
     3541 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
     3601 cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
     3661 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag
     3721 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
     3781 gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc
     3841 atcgaacaca tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg
     3901 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
     3961 gcggttaaga attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga
     4021 aaaaaagctg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct
     4081 gctggtagcg tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa
     4141 agatgtactg catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg
     4201 aaaaactgtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc
     4261 cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
     4321 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct
     4381 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
     4441 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
     4501 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
     4561 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
     4621 ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag
     4681 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
     4741 cgccgccgca ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
     4801 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
     4861 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
     4921 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
     4981 cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa
     5041 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc
     5101 gcccgccagc cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc
     5161 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
     5221 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
     5281 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
     5341 acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg
     5401 ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt
     5461 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
     5521 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
     5581 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
     5641 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
     5701 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt
     5761 gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg
     5821 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
     5881 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat
     5941 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
     6001 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
     6061 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
     6121 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
     6181 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
     6241 gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc
     6301 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
     6361 aatcggtgaa gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat
     6421 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
     6481 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
     6541 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
     6601 gtttacgaaa gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
     6661 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
     6721 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
     6781 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
     6841 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
     6901 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
     6961 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
     7021 caggccgcgc caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc
     7081 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg
     7141 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
     7201 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
     7261 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
     7321 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
     7381 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
     7441 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
     7501 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat
     7561 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
     7621 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgtga tagccgccac
     7681 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
     7741 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
     7801 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
     7861 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
     7921 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
     7981 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
     8041 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
     8101 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
     8161 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
     8221 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa
     8281 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
     8341 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
     8401 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
     8461 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc
     8521 acgcggtggc gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt
     8581 ccagccagct tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt
     8641 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
     8701 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
     8761 acaccagtgg ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa
     8821 cggcgcagca acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg
     8881 gtcgttatag gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc
     8941 gccgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
     9001 cttgccgcct tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc
     9061 aggattttcc gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
     9121 gccgacgtgt gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt
     9181 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
     9241 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
     9301 ggttatctgt ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa
     9361 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
     9421 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
     9481 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
     9541 cagcaaacct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
     9601 gctgacgctt cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga
     9661 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9721 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct
     9781 gtgtcgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9841 ggagcagcat attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg
     9901 gcgacatctc tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc
     9961 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
    10021 ttcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    10081 cggtcagacg cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg
    10141 caaagcggtg aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt
    10201 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10261 gctggagggg attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa
    10321 gcagccagag aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact
    10381 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10441 gcgcagtggt gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc
    10501 caccagcagg atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10561 aggtgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10621 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10681 agaagaaacc agcgaccagg taattgaagc aaaaccgtta ccgaagaatg ccagcgccat
    10741 caggcacata atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat
    10801 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10861 cagcagatcc gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10921 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10981 gaaccaggtt aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc
    11041 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc
    11101 tttcttcact ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    11161 agctttgctg atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    11221 ccagataatg ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg
    11281 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11341 accaacggcg gaggcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt
    11401 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11461 tcccgtggca aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata
    11521 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11581 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11641 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11701 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11761 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11821 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11881 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag
    11941 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc
    12001 ggtttcgtta gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    12061 aagtcgagta actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc
    12121 gcgttatagg aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga
    12181 cagtgcggcg caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg
    12241 gtaataccgc ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc
    12301 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12361 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12421 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12481 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12541 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12601 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc
    12661 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12721 atcagttgcc agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg
    12781 atcccggcaa tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12841 acttgccata agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg
    12901 tgaactgccg cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg
    12961 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    13021 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    13081 gcgcggtgcg ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag
    13141 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    13201 ctgcgatgtc cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13261 atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13321 ccgtcgcgca gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac
    13381 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13441 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13501 cgggaatgct ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13561 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg
    13621 cgatcagcgg aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13681 gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag
    13741 cgcgcggtca acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg
    13801 cgcatactgg cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca
    13861 cggggaagtg ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg
    13921 ggcgtattaa gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc
    13981 ggcaaacctg caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    14041 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    14101 ggcatgacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg
    14161 ccgcgcatca cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata
    14221 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aatgggcagg aacagataaa
    14281 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta
    14341 ttctcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14401 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14461 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14521 cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc
    14581 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14641 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14701 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14761 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14821 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14881 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14941 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    15001 acgcgccgcg ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct
    15061 cacggatgat gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg
    15121 gcgagcctgg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat
    15181 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15241 cgatcgatgc ataatcagga aagtatcaat cgcagtcaga caccatcacg gggctatcgc
    15301 aggcaaagga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    15361 ttatcacaga tggtgccgat gacatcgtta agatgctcaa ggatccagct cttaaccggc
    15421 acaatattca ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    15481 ccggcacaat atcactctat tacaactgga gcacagaaga agaaaaagca cgagtgacac
    15541 aatatcgttt tgcttgtggt gttcagcact gtacccggac agtagaaaac ctggaaggta
    15601 ctatccatga gaaaaataaa aacatggatt actcaaacgt aatggctttc taccatccat
    15661 ttaaagtgcg attttatgaa taccattcac ccagaggcat tccggatggt gtttccgcag
    15721 cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg
    15781 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc
    15841 acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttagat
    15901 ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    15961 acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct
    16021 tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt
    16081 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    16141 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    16201 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatagtg
    16261 gcaacgaaag attcatgcac tgtgtagtag ctgatatcgc ggttggcggt gtacagcgtg
    16321 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    16381 tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagctgc cttctgtact
    16441 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    16501 tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg
    16561 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    16621 gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg tagattggtt
    16681 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    16741 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    16801 tccatacctg cggaaagcag atcgttattg gcttgtggtg acacttcacc ttgcggtgag
    16861 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagctgt actggtatcc
    16921 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc
    16981 tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct
    17041 aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa
    17101 cttaaaatag cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    17161 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatttg
    17221 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    17281 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgaaaatt
    17341 caatacattg caagattttc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg
    17401 gttagcgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac
    17461 ggcgcggttc aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca gcaatcgcat
    17521 ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    17581 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact
    17641 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    17701 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    17761 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    17821 gggtacgcac gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg tggacatcaa
    17881 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    17941 agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa
    18001 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    18061 cgttgtactc agacaccaat ttctctaacg cttcgccagc caatgccggt gcgctggcgt
    18121 tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    18181 cgtctttaat gtactgttct tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    18241 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaaaaag gtcaacagca
    18301 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    18361 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    18421 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt
    18481 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    18541 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    18601 ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac
    18661 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    18721 tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa
    18781 ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    18841 ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    18901 taactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcagg gtacgggtca
    18961 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    19021 agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac
    19081 cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca
    19141 gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct ttgccgtcgc
    19201 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    19261 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    19321 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccaacggt gcctgcggta
    19381 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt
    19441 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    19501 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    19561 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    19621 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    19681 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    19741 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    19801 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc
    19861 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    19921 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    19981 agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    20041 cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    20101 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    20161 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    20221 cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc
    20281 agagaaaact cagggagaaa ttgcttacaa gtatcggcca tatcagccgc gataccggcg
    20341 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagctc tttatcccac
    20401 gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    20461 cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg
    20521 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    20581 aactgcattg gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt accgtcgatg
    20641 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc gcgctcttcg
    20701 ccctgtaatc gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac gcgaccaatc
    20761 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    20821 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    20881 gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    20941 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    21001 aatcggtcag catcatgcgg acgttttcac atttcagcgc gttcagaaca tccagcacgt
    21061 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    21121 ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataaa
    21181 gacgtacgcc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    21241 gcagatcgca gccagcttcc agatgtttgt ccgggttctt cggcagaacg cggcgataat
    21301 ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa
    21361 tgttgttgct gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca
    21421 gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat tgaccaattg
    21481 gcattgaaca gactgccaga cggtggccgt cggtcgccac ggtgcgcagt tcttcgcctt
    21541 cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact
    21601 gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    21661 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    21721 gcccggagcg caccagcatc cgttcacctt ccagctgtac ggcaatttcc gcgccttcag
    21781 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    21841 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    21901 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    21961 cacctaacgg cccgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    22021 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    22081 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    22141 cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc
    22201 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    22261 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    22321 ttttcctgca atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat ggtgatcgcc
    22381 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    22441 ttagatcgta gacgcttggc gataaagaac gccacttcgc ctggcaaacg aatgtcattt
    22501 tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg ttcgatcgcc
    22561 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    22621 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    22681 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    22741 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    22801 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    22861 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    22921 ccataaagga acaatgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc
    22981 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    23041 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    23101 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    23161 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    23221 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    23281 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    23341 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    23401 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    23461 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    23521 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    23581 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    23641 cgatttatcc acaggacttt ccagacctcg ctaagtgtaa acgatcctgc cgcgaggcgg
    23701 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    23761 atctaataaa gatctaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    23821 ccggcgcgcc atgctggttt tcactggtgt gaggtcgtac attttccctg cgaaaaggtg
    23881 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc
    23941 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    24001 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    24061 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    24121 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    24181 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    24241 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    24301 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    24361 aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt
    24421 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    24481 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    24541 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    24601 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    24661 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    24721 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    24781 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    24841 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    24901 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    24961 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    25021 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct
    25081 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    25141 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    25201 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    25261 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    25321 gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat
    25381 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg
    25441 cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga
    25501 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc aacagtattt
    25561 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    25621 taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    25681 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    25741 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    25801 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    25861 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    25921 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    25981 caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    26041 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    26101 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    26161 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    26221 gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    26281 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag
    26341 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    26401 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    26461 agttgaccgc cttttttctt ttcgtagggt ggataagcac cgcgcatccg ccacacaaag
    26521 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    26581 tggcggcgtt ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg ccgaaaccgt
    26641 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    26701 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    26761 agatgtgctg gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    26821 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    26881 tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    26941 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    27001 caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    27061 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    27121 gattgccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    27181 gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct
    27241 ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg
    27301 tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga
    27361 agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    27421 cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat
    27481 ctggccggaa tttatcgccc gtctgccagc gaaactaccg atcaccgtgg tgcgcaataa
    27541 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    27601 tctctcggca aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca aacagagcat
    27661 gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct
    27721 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg cctgggcggg
    27781 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    27841 atttacctct gatgatttgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    27901 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    27961 gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    28021 aaagcattta atattgctcc c
//
LOCUS       DAJVYM010000039        27593 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVYM010000039 DAJVYM010000000
VERSION     DAJVYM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.039"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            54..1274
                     /locus_tag="OQS60_001596"
                     /pseudo
     CDS             54..1274
                     /locus_tag="OQS60_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="OQS60_001597"
     CDS             1247..1876
                     /locus_tag="OQS60_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCS2387379.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OQS60_001598"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OQS60_001598"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS2387380.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="OQS60_001599"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="OQS60_001599"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS2387381.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="OQS60_001600"
     CDS             complement(4244..4441)
                     /locus_tag="OQS60_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS2387382.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OQS60_001601"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OQS60_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS2387383.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OQS60_001602"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OQS60_001602"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS2387384.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OQS60_001603"
     CDS             complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OQS60_001603"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS2387385.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OQS60_001604"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OQS60_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS2387386.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OQS60_001605"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OQS60_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS2387387.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OQS60_001606"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OQS60_001606"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS2387388.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="OQS60_001607"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="OQS60_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS2387389.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OQS60_001608"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OQS60_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS2387390.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="OQS60_001609"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="OQS60_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS2387391.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="OQS60_001610"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="OQS60_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS2387392.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="OQS60_001611"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="OQS60_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS2387393.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OQS60_001612"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OQS60_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS2387394.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLLSFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OQS60_001613"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OQS60_001613"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS2387395.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OQS60_001614"
     CDS             complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OQS60_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS2387396.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OQS60_001615"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OQS60_001615"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS2387397.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="OQS60_001616"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="OQS60_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS2387398.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="OQS60_001617"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="OQS60_001617"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS2387399.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OQS60_001618"
     CDS             complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OQS60_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS2387400.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         6601 a         7724 c         7176 g         6092 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagagcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc agg
//
LOCUS       DAJVYM010000040        27166 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVYM010000040 DAJVYM010000000
VERSION     DAJVYM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.040"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            38..580
                     /locus_tag="OQS60_001619"
     CDS             38..580
                     /locus_tag="OQS60_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2387401.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="OQS60_001620"
     CDS             663..1364
                     /locus_tag="OQS60_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2387402.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="OQS60_001621"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="OQS60_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="OQS60_001622"
     CDS             3979..5049
                     /locus_tag="OQS60_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2387403.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="OQS60_001623"
     CDS             5061..5603
                     /locus_tag="OQS60_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2387404.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="OQS60_001624"
     CDS             5611..6126
                     /locus_tag="OQS60_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2387405.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="OQS60_001625"
     CDS             6092..6829
                     /locus_tag="OQS60_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS2387406.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="OQS60_001626"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="OQS60_001626"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS2387407.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="OQS60_001627"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="OQS60_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS2387408.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="OQS60_001628"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="OQS60_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS2387409.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="OQS60_001629"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="OQS60_001629"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS2387410.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="OQS60_001630"
     CDS             12136..14043
                     /locus_tag="OQS60_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2387411.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="OQS60_001631"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="OQS60_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS2387412.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="OQS60_001632"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="OQS60_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS2387413.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="OQS60_001633"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="OQS60_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS2387414.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="OQS60_001634"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="OQS60_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS2387415.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="OQS60_001635"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="OQS60_001635"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS2387416.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="OQS60_001636"
     CDS             complement(18711..20471)
                     /locus_tag="OQS60_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS2387417.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="OQS60_001637"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="OQS60_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS2387418.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="OQS60_001638"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="OQS60_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS2387419.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="OQS60_001639"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="OQS60_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS2387420.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="OQS60_001640"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="OQS60_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS2387421.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="OQS60_001641"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="OQS60_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS2387422.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="OQS60_001642"
     CDS             complement(26072..26518)
                     /locus_tag="OQS60_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS2387423.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..>27166
                     /gene="helD"
                     /locus_tag="OQS60_001643"
     CDS             26641..>27166
                     /gene="helD"
                     /locus_tag="OQS60_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="catalyzes the ATP-dependent unwinding of duplex DNA
                     in the 3' to 5' direction with respect to the bound single
                     strand; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS2387424.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQ"
BASE COUNT         7017 a         6402 c         7105 g         6642 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagttc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accagg
//
LOCUS       DAJVYM010000041        26832 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVYM010000041 DAJVYM010000000
VERSION     DAJVYM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.041"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1006)
                     /gene="rsmF"
                     /locus_tag="OQS60_001644"
     CDS             complement(<1..1006)
                     /gene="rsmF"
                     /locus_tag="OQS60_001644"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS2387425.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     R"
     gene            complement(1087..3720)
                     /gene="yebT"
                     /locus_tag="OQS60_001645"
     CDS             complement(1087..3720)
                     /gene="yebT"
                     /locus_tag="OQS60_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS2387426.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(3689..4972)
                     /gene="yebS"
                     /locus_tag="OQS60_001646"
     CDS             complement(3689..4972)
                     /gene="yebS"
                     /locus_tag="OQS60_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS2387427.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5102..5599
                     /gene="msrC"
                     /locus_tag="OQS60_001647"
     CDS             5102..5599
                     /gene="msrC"
                     /locus_tag="OQS60_001647"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS2387428.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            5696..6394
                     /gene="proQ"
                     /locus_tag="OQS60_001648"
     CDS             5696..6394
                     /gene="proQ"
                     /locus_tag="OQS60_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS2387429.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6414..8462
                     /gene="prc"
                     /locus_tag="OQS60_001649"
     CDS             6414..8462
                     /gene="prc"
                     /locus_tag="OQS60_001649"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS2387430.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            8654..9535
                     /gene="htpX"
                     /locus_tag="OQS60_001650"
     CDS             8654..9535
                     /gene="htpX"
                     /locus_tag="OQS60_001650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS2387431.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(9581..10644)
                     /locus_tag="OQS60_001651"
                     /pseudo
     CDS             complement(9581..10644)
                     /locus_tag="OQS60_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10821..11612
                     /gene="kdgR"
                     /locus_tag="OQS60_001652"
     CDS             10821..11612
                     /gene="kdgR"
                     /locus_tag="OQS60_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS2387432.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(11755..11994)
                     /locus_tag="OQS60_001653"
     CDS             complement(11755..11994)
                     /locus_tag="OQS60_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387433.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12153..12296
                     /gene="mgrB"
                     /locus_tag="OQS60_001654"
     CDS             12153..12296
                     /gene="mgrB"
                     /locus_tag="OQS60_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS2387434.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12371..12658
                     /locus_tag="OQS60_001655"
     CDS             12371..12658
                     /locus_tag="OQS60_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS2387435.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13328..13471
                     /locus_tag="OQS60_001656"
     CDS             13328..13471
                     /locus_tag="OQS60_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS2387436.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            13484..13693
                     /gene="cspE"
                     /locus_tag="OQS60_001657"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             13484..13693
                     /gene="cspE"
                     /locus_tag="OQS60_001657"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS2387437.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            13859..14668
                     /gene="rlmA"
                     /locus_tag="OQS60_001658"
     CDS             13859..14668
                     /gene="rlmA"
                     /locus_tag="OQS60_001658"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS2387438.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(14665..15231)
                     /gene="mntP"
                     /locus_tag="OQS60_001659"
     CDS             complement(14665..15231)
                     /gene="mntP"
                     /locus_tag="OQS60_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS2387439.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(15660..16118)
                     /locus_tag="OQS60_001660"
     CDS             complement(15660..16118)
                     /locus_tag="OQS60_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS2387440.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16173..17024)
                     /gene="manZ"
                     /locus_tag="OQS60_001661"
     CDS             complement(16173..17024)
                     /gene="manZ"
                     /locus_tag="OQS60_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS2387441.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17037..17837)
                     /gene="manY"
                     /locus_tag="OQS60_001662"
     CDS             complement(17037..17837)
                     /gene="manY"
                     /locus_tag="OQS60_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS2387442.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(17900..18871)
                     /gene="manX"
                     /locus_tag="OQS60_001663"
     CDS             complement(17900..18871)
                     /gene="manX"
                     /locus_tag="OQS60_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS2387443.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19334..20884
                     /gene="yoaE"
                     /locus_tag="OQS60_001664"
     CDS             19334..20884
                     /gene="yoaE"
                     /locus_tag="OQS60_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS2387444.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(20888..22486)
                     /locus_tag="OQS60_001665"
     CDS             complement(20888..22486)
                     /locus_tag="OQS60_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS2387445.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(22617..23981)
                     /gene="sdaA"
                     /locus_tag="OQS60_001666"
     CDS             complement(22617..23981)
                     /gene="sdaA"
                     /locus_tag="OQS60_001666"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS2387446.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24165..24743)
                     /locus_tag="OQS60_001667"
     CDS             complement(24165..24743)
                     /locus_tag="OQS60_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS2387447.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(24747..26108)
                     /gene="pabB"
                     /locus_tag="OQS60_001668"
     CDS             complement(24747..26108)
                     /gene="pabB"
                     /locus_tag="OQS60_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS2387448.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26182..26361
                     /locus_tag="OQS60_001669"
     CDS             26182..26361
                     /locus_tag="OQS60_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS2387449.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(26481..26804)
                     /locus_tag="OQS60_001670"
     CDS             complement(26481..26804)
                     /locus_tag="OQS60_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS2387450.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         6929 a         6586 c         6786 g         6531 t
ORIGIN      
        1 ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg gcgggtaagg
       61 cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag tcgtaaattt
      121 gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca gggaagagat
      181 caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac aggcaaacgg
      241 cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg ccagggcgta
      301 atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc tgattgcttt
      361 ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg ccggagcagg
      421 gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac acgcggccat
      481 caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca tgtaacactt
      541 ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc atccgcgcgg
      601 aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc tgtggtgcat
      661 taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga atataaaaca
      721 ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg tcgcgttcaa
      781 tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa ggagcggtta
      841 attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg cggcgcaacg
      901 ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc gcttcgcgca
      961 tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc atataccact
     1021 ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac gccggagcag
     1081 tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt cactttcctg
     1141 caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg gaggcgtggc
     1201 gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta cgccgcccgt
     1261 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     1321 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     1381 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccaatacc
     1441 taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt aacgcgaatc
     1501 agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac ctggttcgac
     1561 attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg aaatttgcgg
     1621 tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact ccggatagag
     1681 caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct ggatttgccc
     1741 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     1801 gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     1861 tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc taatggctcc
     1921 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     1981 tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc gatactccgg
     2041 cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa tcacttcacc
     2101 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctctgc
     2161 actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     2221 attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca gaatacgtat
     2281 cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat ccagccccac
     2341 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttcgcgatg
     2401 ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac gatcaataac
     2461 ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac cgtaactttc
     2521 cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt tttcgcctgg
     2581 cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga aggttttgcc
     2641 agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa gtaattcgat
     2701 gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt ccccagtgac
     2761 tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc cctgatacat
     2821 taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt tgattattac
     2881 gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg cctcggcagg
     2941 tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg ccagactttc
     3001 cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa cgttccagaa
     3061 acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat caatcaccac
     3121 gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga tcttgcggaa
     3181 atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt ggatcatcag
     3241 atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt gatcctgctc
     3301 tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt ccagcccgga
     3361 gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt cgcgcagcgc
     3421 atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat cgtcgctgag
     3481 gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg tacggcccgg
     3541 gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt cctgataact
     3601 gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc agaaaggtga
     3661 gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt cctgactcat
     3721 gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc aaaattacgg
     3781 ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga tcgcgattaa
     3841 tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt ccaatccagg
     3901 tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta aaatgaatgc
     3961 tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata ctggcgataa
     4021 acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg gaaagaatcg
     4081 tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg ttggctggta
     4141 acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta tgacgacgac
     4201 gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga tagccggtaa
     4261 aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg cgctgcggat
     4321 aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc gttaaaatca
     4381 ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa tcctgtacct
     4441 ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac tctttaagac
     4501 gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca aaccataaat
     4561 atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag accatcgccc
     4621 ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg ttggcgtcga
     4681 tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac ggcatcagca
     4741 acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa
     4801 tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg ggcaggctaa
     4861 acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc tcgccgattg
     4921 ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc
     4981 aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt gagcattaac
     5041 gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa gtgattatat
     5101 aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc tgatggcggg
     5161 agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac
     5221 tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac tcggaccatt
     5281 tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg gcactgcggt
     5341 tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc atattgcctg
     5401 tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc agattattgg
     5461 tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg agcaaggctt
     5521 acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca aaaaattctt
     5581 tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg tcattataat
     5641 gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa atttcatgga
     5701 aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg aacgttttcc
     5761 ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt ttcaggattt
     5821 ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat ccgctttacg
     5881 tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga
     5941 tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg ctcgcaagca
     6001 gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg
     6061 cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac gcaagccacg
     6121 tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt
     6181 agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc cggtttctga
     6241 catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga
     6301 tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt cgggtatgtc
     6361 tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca
     6421 tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag accttcgctg
     6481 tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg cagcatgcga
     6541 cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag ttcgacctcg
     6601 atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat tacagccaca
     6661 acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg
     6721 aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg caaaagcgcc
     6781 gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat ttcaccggca
     6841 acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag gctgagttga
     6901 acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg gcaggaaaaa
     6961 cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt cgtcgtctgg
     7021 cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg cgtgaaatcg
     7081 acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact gaaatgagtt
     7141 tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc gttatcaatt
     7201 cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt gacaaaattg
     7261 tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt cttgatgatg
     7321 tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt ttacctgctg
     7381 gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt ctcgaagacc
     7441 gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc
     7501 cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa ctggaaaaac
     7561 agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag
     7621 cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc cgcgataaca
     7681 acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa ggcccgctgg
     7741 tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg
     7801 attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat
     7861 accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt
     7921 ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg caacgtaaag
     7981 gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat
     8041 tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg aaatcaggcg
     8101 atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc gcgaaagatc
     8161 ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg gacaagcgca
     8221 atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc
     8281 gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg aagaagctgg
     8341 atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg gtgaatatcg
     8401 cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct cccgtcaagt
     8461 aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag atgccgtaaa
     8521 tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta tccagacttg
     8581 aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt taaactgagg
     8641 taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt
     8701 cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc aggggctgat
     8761 gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg
     8821 gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg
     8881 ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta tgccgcaagt
     8941 ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc
     9001 tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag ccgaggcggt
     9061 aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga cgctgattca
     9121 gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg
     9181 ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc tgatctactt
     9241 tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta tcaccatgtg
     9301 gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg ttggtcgcga
     9361 gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag aagcaaccag
     9421 catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt tcatgactca
     9481 cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga agtaatatga
     9541 agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg gatcgtggct
     9601 gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc gcagccatca
     9661 gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc agtgccgcgc
     9721 cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca ccgctacgtt
     9781 cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag ccaaatccag
     9841 cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg ggcagcagaa
     9901 ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc aatcctgcat
     9961 gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct aacggccacg
    10021 gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc aggtaaaagg
    10081 gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg caaatagaaa
    10141 gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga agagaaagct
    10201 ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca gcaattaacg
    10261 tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg aaggttaacg
    10321 cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg
    10381 gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg attaaaaata
    10441 accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg gcagcagaag
    10501 agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc cgtcaaggac
    10561 agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg gcaatggcag
    10621 gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa tcaacagggg
    10681 tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa ccggcaaaag
    10741 atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat ttttataaaa
    10801 cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct gattctgtat
    10861 cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc gaaataggga
    10921 taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc tttttacaga
    10981 ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat tccctgaccc
    11041 tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt cgtagcgcag
    11101 atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc ggcgcactgg
    11161 acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg cgcatgtatt
    11221 cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta ctgctggcat
    11281 ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa cgcagtaccg
    11341 agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg cgcgagcagg
    11401 ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg gtaccggtat
    11461 ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg ttgcgtttct
    11521 ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc aaaatttctg
    11581 cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc ggaatatcaa
    11641 acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt atattaaacg
    11701 cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt acccttaacc
    11761 attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc ccaagaaacc
    11821 acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat aggtacattg
    11881 caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc cgctgagtaa
    11941 taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc gcattacaac
    12001 ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc aagctactca
    12061 ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc atgctagttt
    12121 aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg gtcgttctgg
    12181 ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg atgtgcgatc
    12241 aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg tggtgatatc
    12301 attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc ctctactcag
    12361 aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt ctttggttgt
    12421 atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc gtgccagttc
    12481 acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa aacgtcaaaa
    12541 tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata aaaagaccat
    12601 tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg aacgataatc
    12661 cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc tgattatacg
    12721 cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa taattatcat
    12781 attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa tttattagga
    12841 tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg tttctctctt
    12901 gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct ttggggattg
    12961 acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa aagtgatata
    13021 actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta ataacttctc
    13081 ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc agtttaagta
    13141 tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag
    13201 tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa
    13261 atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac gaggtacagt
    13321 tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg acgttgaata
    13381 ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat
    13441 gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg
    13501 tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg ctgatggcag
    13561 caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa ctctggctga
    13621 aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag ctgttaacgt
    13681 aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa
    13741 agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc gttcggatat
    13801 ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat
    13861 gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg
    13921 tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc tgtccgttca
    13981 gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt
    14041 cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac tgagggaacg
    14101 gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt attacacaca
    14161 cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt cgaaggtagc
    14221 gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt ccagccaccg
    14281 tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc cgtgtaaagc
    14341 agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca cgccgggacc
    14401 gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc atgcacctca
    14461 tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt atccgatgcg
    14521 tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct ggcgtgcgaa
    14581 gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga cggactttaa
    14641 tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc
    14701 gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat
    14761 aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac caatggccaa
    14821 tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag ccatggcatc
    14881 caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac ggcgcggctc
    14941 ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat
    15001 cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg ctaacatgcc
    15061 cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg
    15121 caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg atgcagcaaa
    15181 tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca tgacaatgtc
    15241 ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac
    15301 aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc
    15361 tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca
    15421 tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga
    15481 gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac gattttcatt
    15541 tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga ggtaatatag
    15601 cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa attcataact
    15661 tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt tcgaacgcgg
    15721 attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga caagttcatc
    15781 gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt ttgtttaaag
    15841 atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag tgcgcttaat
    15901 aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag aatgacaatc
    15961 aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat tgccagcagg
    16021 gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc caggagtgcg
    16081 gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga taaaaaaacg
    16141 gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc gcaagcgtaa
    16201 ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac ttttttgcgc
    16261 agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat taactggtcc
    16321 agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat gcgagagaca
    16381 accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac aaacaggccg
    16441 aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc tttaacgata
    16501 tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag acgcaccagg
    16561 ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc gataccggca
    16621 cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc tacaccagcc
    16681 agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat ctctgcgcca
    16741 ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc cacgaacggc
    16801 tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc gttgttctca
    16861 gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc ctgcatacgt
    16921 tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg aatatcactt
    16981 tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct cacctgttag
    17041 tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc ggctacgcgg
    17101 ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat cacacccaga
    17161 gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa gaacggcatc
    17221 aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac gatcatgcca
    17281 ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat gttctgtact
    17341 tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg cattgcttgc
    17401 aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt atcagcagcc
    17461 ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt cagtacctgg
    17521 cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg atgacctgcg
    17581 ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac tgcagcaccg
    17641 atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat accggttttc
    17701 atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg aaactgaaat
    17761 tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag cacaatttga
    17821 agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt gaaaactgag
    17881 tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc attttcagtt
    17941 tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc agtttcttga
    18001 acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt ttaccctgac
    18061 ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca acgagacgct
    18121 ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat ttcgggttgt
    18181 tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc ggaggtgcaa
    18241 cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta acaacaataa
    18301 tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga atcagacggt
    18361 cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt tttgccggag
    18421 ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca accggtttgg
    18481 ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc agttcatcaa
    18541 agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg ttaacgcctg
    18601 caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg aacgggctgc
    18661 ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt gccaactgag
    18721 cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg atccagccga
    18781 cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct gcagcccaac
    18841 catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat cgttaacacc
    18901 tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc agataagggc
    18961 tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa ttattttatg
    19021 tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta aaatatcctt
    19081 gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc tctgatatgt
    19141 ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg gatcgtcacc
    19201 gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta tgccgcacct
    19261 ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc tattccttaa
    19321 gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc tactcacgct
    19381 tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca ttcttgctga
    19441 caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac tggcgctgat
    19501 tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca aaccgctatt
    19561 taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg gggggatatt
    19621 cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg atcatgattc
    19681 cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg tcatccttga
    19741 cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc atctgccggt
    19801 gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca aaccgctgac
    19861 gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc tgttaatgat
    19921 tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt acctgtatgc
    19981 cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc gcaactttat
    20041 tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc tgcgtttgat
    20101 gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc cgatcccgga
    20161 aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc ttgcgtcgcg
    20221 ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg ctaatctcgg
    20281 ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc cggtatgtcg
    20341 cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg tggcgctgga
    20401 agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg tcccggaaac
    20461 cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct ttgttatcgt
    20521 gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc tggaagccat
    20581 tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg gtgacggctg
    20641 gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg ttgagcacct
    20701 tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa atggtcacat
    20761 tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca ttgaagccaa
    20821 tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg atgaagatga
    20881 gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg gcgggcggcg
    20941 gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt gcgtacaaat
    21001 acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct tgcgtttcca
    21061 cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg atatcggtca
    21121 ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca attttcagca
    21181 tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg aagtcatcaa
    21241 tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca acatccagta
    21301 aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg ctaaaccagt
    21361 actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg acgttaatgc
    21421 caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt tctgccatca
    21481 catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata aacacatccg
    21541 gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca ccaatacatt
    21601 gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt tgcgccaggc
    21661 ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag gcgatgtagc
    21721 ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag tgccatgcca
    21781 gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc gaagaaacct
    21841 gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta ccatagagca
    21901 aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt ggctcaagga
    21961 gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg ctactgcccg
    22021 ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg tcgatcgtca
    22081 ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg ggcacattgc
    22141 gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca atggatcgca
    22201 cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag cagggcagac
    22261 caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact ttatctaatt
    22321 cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta cgctgtgaaa
    22381 taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta caaacaatca
    22441 ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg gccaccctgt
    22501 aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa tattacagca
    22561 gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa gaagtattag
    22621 tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt ggcgttcatg
    22681 tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg cggtgcactg
    22741 gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat ggcattacgc
    22801 tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc aaggttgtgt
    22861 tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag cagttctgca
    22921 agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc aacttccgca
    22981 ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc cataaagtaa
    23041 cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc cagcactgcc
    23101 ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc ggcggcgttt
    23161 tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat cggatcgtta
    23221 gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg tggcacgcgc
    23281 agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc ctgcatggtt
    23341 tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc cagttcgttc
    23401 tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag cagttcggtg
    23461 gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc aaagtgttct
    23521 tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac gacttcatcg
    23581 ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc gttatgaaaa
    23641 cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag cagcagacgt
    23701 tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac ggtggcaggt
    23761 tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt acccgtcagc
    23821 gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa gcctttttcg
    23881 accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg ggaagatgag
    23941 ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact cctgacaagg
    24001 tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga acgcggcgac
    24061 tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact aatattgcga
    24121 tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg tttcacacca
    24181 atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa atactgttcg
    24241 taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa agggtgataa
    24301 cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac ttcatcttca
    24361 ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac ctgatagcca
    24421 gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg cggtatagcg
    24481 acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt gtcatcgact
    24541 gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga acgctgagtc
    24601 agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc ctgacgatga
    24661 tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa aaaatcatca
    24721 agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat cttattaact
    24781 ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat tccaccgccc
    24841 gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat gctggtatcc
    24901 atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg tcgctgcggt
    24961 tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat tgagccacca
    25021 ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg tgccgttatg
    25081 gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc tggtactttt
    25141 accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac aatcatcaga
    25201 ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct atcttcctga
    25261 ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc actattatca
    25321 caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag acgtaaaaaa
    25381 gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca ttcatcgcca
    25441 gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc gctgtgcaga
    25501 tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt ggattgccag
    25561 tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag ccaggcccga
    25621 cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg gtcgacaatg
    25681 agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg ttccgcaatt
    25741 tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag tgcgccgccc
    25801 tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag cacctgctgg
    25861 agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact aacaacggtt
    25921 tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc aaagcggcta
    25981 tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg gcttaagcgg
    26041 gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc gggagataac
    26101 gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata attagcgcca
    26161 acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca tgaacagcag
    26221 caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag cggccaggca
    26281 atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat cgtggcgcgt
    26341 tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac aagccgatga
    26401 ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc ctgatccatt
    26461 tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat atatgttgaa
    26521 aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt tgcccatcca
    26581 accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc cgtaatatca
    26641 gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt cgccgtgctt
    26701 tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa atccagggct
    26761 ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc gataaggcta
    26821 tatatgacct ta
//
LOCUS       DAJVYM010000042        26766 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVYM010000042 DAJVYM010000000
VERSION     DAJVYM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.042"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            110..382
                     /locus_tag="OQS60_001671"
     CDS             110..382
                     /locus_tag="OQS60_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS2387451.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            475..558
                     /locus_tag="OQS60_001672"
     CDS             475..558
                     /locus_tag="OQS60_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387452.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            658..1479
                     /locus_tag="OQS60_001673"
     CDS             658..1479
                     /locus_tag="OQS60_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS2387453.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OQS60_001674"
     CDS             complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OQS60_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS2387454.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OQS60_001675"
     CDS             complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OQS60_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS2387455.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2611..3645
                     /gene="tqsA"
                     /locus_tag="OQS60_001676"
     CDS             2611..3645
                     /gene="tqsA"
                     /locus_tag="OQS60_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS2387456.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OQS60_001677"
     CDS             complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OQS60_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS2387457.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OQS60_001678"
     CDS             complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OQS60_001678"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS2387458.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7125..8069
                     /locus_tag="OQS60_001679"
     CDS             7125..8069
                     /locus_tag="OQS60_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2387459.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8255..9637
                     /locus_tag="OQS60_001680"
     CDS             8255..9637
                     /locus_tag="OQS60_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2387460.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9674..10396
                     /gene="folM"
                     /locus_tag="OQS60_001681"
     CDS             9674..10396
                     /gene="folM"
                     /locus_tag="OQS60_001681"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS2387461.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10393..10728)
                     /locus_tag="OQS60_001682"
     CDS             complement(10393..10728)
                     /locus_tag="OQS60_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS2387462.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10857..11576
                     /gene="rstA"
                     /locus_tag="OQS60_001683"
     CDS             10857..11576
                     /gene="rstA"
                     /locus_tag="OQS60_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS2387463.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11580..12881
                     /gene="rstB"
                     /locus_tag="OQS60_001684"
     CDS             11580..12881
                     /gene="rstB"
                     /locus_tag="OQS60_001684"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS2387464.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12957..13886
                     /gene="tus"
                     /locus_tag="OQS60_001685"
     CDS             12957..13886
                     /gene="tus"
                     /locus_tag="OQS60_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS2387465.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="OQS60_001686"
     CDS             complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="OQS60_001686"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS2387466.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="OQS60_001687"
     CDS             complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="OQS60_001687"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS2387467.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17274..18449
                     /gene="manA"
                     /locus_tag="OQS60_001688"
     CDS             17274..18449
                     /gene="manA"
                     /locus_tag="OQS60_001688"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS2387468.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18550..20058
                     /locus_tag="OQS60_001689"
     CDS             18550..20058
                     /locus_tag="OQS60_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS2387469.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="OQS60_001690"
     CDS             complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="OQS60_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS2387470.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="OQS60_001691"
     CDS             complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="OQS60_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS2387471.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="OQS60_001692"
     CDS             complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="OQS60_001692"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS2387472.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="OQS60_001693"
     CDS             complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="OQS60_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS2387473.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="OQS60_001694"
     CDS             complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="OQS60_001694"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS2387474.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6715 a         6937 c         6433 g         6681 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca
      301 aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc
      361 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
      421 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
      481 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
      541 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
      601 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
      661 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
      721 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
      781 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
      841 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
      901 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt
      961 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
     1021 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     1081 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg
     1141 ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa
     1201 gtaactcagg cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     1261 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     1321 gacagcggtt cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa
     1381 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     1441 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg
     1501 tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     1561 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     1621 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     1681 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     1741 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     1801 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     1861 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
     1921 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     1981 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt
     2041 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     2101 gccgccattt ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt
     2161 agcaatagcc aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     2221 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata
     2281 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     2341 gcaagatagt ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat
     2401 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     2461 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     2521 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     2581 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
     2641 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     2701 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     2761 gtgcaacaca tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc
     2821 atcatcgtga tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg
     2881 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     2941 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg
     3001 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     3061 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     3121 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt
     3181 gacagtgttt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
     3241 gcctgggcga tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     3301 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
     3361 caggtactgg tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg
     3421 attaatctgg tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt
     3481 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     3541 atgctgattt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     3601 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     3661 ccgtcagggt tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag
     3721 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     3781 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     3841 aataggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     3901 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     3961 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     4021 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     4081 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     4141 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc
     4201 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     4261 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
     4321 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     4381 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
     4441 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     4501 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     4561 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
     4621 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     4681 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     4741 atagctgtta aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac
     4801 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     4861 aatgaccatc gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc
     4921 gattaacgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     4981 tttcgaaaga ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     5041 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     5101 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     5161 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     5221 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca
     5281 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     5341 gcgcaacgct tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     5401 gccacggtga gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
     5461 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     5521 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
     5581 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg
     5641 tcggcagacg gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga
     5701 agatttcacc cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa
     5761 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     5821 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     5881 gttccatttc cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccactgc
     5941 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     6001 tcacttccgg acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     6061 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     6121 cggccgcagt aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
     6181 cgcgataacc ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     6241 tacgcggcac agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     6301 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     6361 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     6421 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     6481 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
     6541 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
     6601 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     6661 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     6721 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     6781 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     6841 ctgataattg tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact
     6901 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
     6961 ttttttaagc atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg
     7021 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
     7081 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
     7141 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
     7201 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
     7261 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
     7321 cctcttttta tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg
     7381 ctgacctcta taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
     7441 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
     7501 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
     7561 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccaa ggcggcaacc
     7621 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
     7681 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
     7741 cgaagaaagt tgagatccta ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
     7801 gtttctttga aacccagtca tcaaaaggcg ggcgttacac cgtcatgctc ccggatggca
     7861 ctaaagtcga agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca
     7921 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg
     7981 cgaagaaagg tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc
     8041 tgaccgtcag cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat
     8101 gccgcctttc gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc
     8161 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
     8221 attcttctta tcacgtttta atcactggat atcgatgaaa aagaaactgg gactgagcgc
     8281 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
     8341 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
     8401 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
     8461 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
     8521 gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
     8581 gttaagcttt tttacggaca cgccggaatt gcgcctgttc ggcgatggta atacctggca
     8641 atcgatcgtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
     8701 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
     8761 gtttgttgtg ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg
     8821 acttgcactt ggcgtacccg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
     8881 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
     8941 tgttggcaaa gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac
     9001 gctgctttcg ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat
     9061 ggccggtctg atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt
     9121 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
     9181 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
     9241 gccatccgcc tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg
     9301 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
     9361 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
     9421 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
     9481 gctgttatcc gttgttctgt atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac
     9541 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
     9601 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc
     9661 aaaggagata acgatgggta aagcccagcc cttgccaata ttaattactg gcggaggtcg
     9721 tcgcatcggc ctcgccctcg cctggcattt cattaatcaa aagcaaccgg tgattgtcag
     9781 ctatcggacg cactatccag ccattgatgg actgattaag gcaggtgcgc agtgtattca
     9841 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagccc
     9901 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
     9961 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    10021 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    10081 ctttaccgat tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa
    10141 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    10201 gaactctatt gctccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    10261 acaggcgctg aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    10321 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    10381 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    10441 ccaacacgaa acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    10501 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    10561 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt
    10621 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    10681 caacagcact accagcgcac cgagcgcggc tttgattacc agccccattt ttttacctta
    10741 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    10801 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    10861 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg
    10921 caaaacatga tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    10981 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    11041 ccatttgtcg tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg
    11101 atagcgatat gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    11161 cgacgccccc tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    11221 ccacactgac caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg
    11281 gcacgttgac catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    11341 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    11401 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg
    11461 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc
    11521 gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga
    11581 tgaaaaaact gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc
    11641 tgctggttgg gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    11701 atttgatgaa cagttcactg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg
    11761 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc
    11821 cactgagtaa ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg
    11881 tcgccctgga cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg
    11941 cagttggtcc tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc
    12001 tgatcacttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    12061 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    12121 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    12181 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    12241 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    12301 gcgccgccga atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag
    12361 agctgctgac ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag
    12421 acctgccgtt gtggctgtca acgcatctgg cagacattca ggcagtaacg cccgataaaa
    12481 cggtacggat taaaacgctc gcgcaaggcc attatgcggc gttggatatg cgcttaatgg
    12541 agcgcgtgct ggataatttg ctcaataacg ccctgcggta ctgccattca acggttgaaa
    12601 ccagcctgct actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga
    12661 ttgccccaga aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg
    12721 atcgctcaac cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa
    12781 tgggcggtac ggttaattgt gacaccagcg aactgggtgg tgctcgcttc tcgtttagct
    12841 ggccgttatg gcataacatc ccgcaattta cctctgcctg acactacgcg cacgatggtc
    12901 aagtcaccac cactgtgcta taaaataagt atgttgtaac taaagtggtt aatattatgg
    12961 cgcgttacga tctcgtagac cgactcaaca ctacctttcg ccagatggaa caagagctgg
    13021 ctgcatttgc cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc
    13081 cggaggtaaa aaaagaggat gagcataatc cgcttaatcg tattgaggta aaacaacatc
    13141 tcggcaacga cgcgcagtcg ctggcgttgc gtcatttccg ccatttattt attcaacaac
    13201 agtccgaaaa tcgcagcagc aaggccgctg tccgtctgcc tggcgtgttg tgttaccagg
    13261 tcgataacct ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga
    13321 ccacgttcga gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat
    13381 gggtgcatcg tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc
    13441 tgcacgaccc cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc
    13501 atcgtgatga agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac
    13561 cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc
    13621 tgccacagaa cgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg
    13681 tctggtacaa aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac
    13741 tgattaatcg ggataatggc gcgggcgtgc cggacgttgg tgagttgtta aattacgatg
    13801 ccgacaatgt gcagcaccgt tataaaccac aggcgcagcc gcttcgtttg atcattccac
    13861 ggctgcacct gtatgttgcg gattaacgcc cggctttcat actgccgacc atctgttctg
    13921 gccgtaccca gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt
    13981 ttaaggtcag cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa
    14041 tgtgggtgtt aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct
    14101 cacgattcgg ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca
    14161 gcaagcgcac cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa
    14221 agttaccgga agcgccaccc atgttgatcg ccacgtcgtt ccccattacc tgacagcaga
    14281 gcatggttaa tgcttcacac tgggttgggt tcactttccc cggcatgatt gagctgcccg
    14341 gctcattttc cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc
    14401 ggacatcatt ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct
    14461 gaaccagggc atcacaggtc gcaagcgctt caaatttgtt cggcgcggta acaaacggtg
    14521 cacaggtaat gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta
    14581 gtccagtacc caccgctgta ccgccgagag ccagttccgc tacgtgaggc aggctgtatt
    14641 cgatatgttt gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg
    14701 ttagcggcgt ggcgtcctgc aagtgggttc gaccgatttt gacgatatcg gcaaatgcac
    14761 gcgatttttc actcagtgtc tgtgtcaggg ttttaacctg cggaatgagt tgcttgcgca
    14821 gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt
    14881 tgttcacgtc gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt
    14941 cactggcccg gttagccagc acttcgttca tgttcatgtt actttgcgtg ccggagccgg
    15001 tctgccagat agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg
    15061 cctgccgaat ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg
    15121 ccgctgcgcg cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg
    15181 aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga
    15241 catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tgctcctcac
    15301 ctgattaatt ttttctttct gttttgcctt cgttaagcaa cttttagctc acttattatt
    15361 tactatttga taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc
    15421 ggctctgttt atttcacaca gcgagtgcac tgtgtgagtt gtatctgctg gaagaagtca
    15481 tttcctttat catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg
    15541 gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc
    15601 aatacagcgg ccggaccacc gatactgcca aggtagaagc cgccgtgttt tttacaggca
    15661 tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc gccctgcgct
    15721 tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag
    15781 gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac
    15841 tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga
    15901 ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt
    15961 gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct
    16021 ttacgcagct cttcagggat atatttacct ggattatgtt ccagtttttc gatccagatc
    16081 ccctgacggt tgatcttcgc tttgatatta cggtcagcag agcaggaaac acccatacca
    16141 accggacagg atgcgccgtg acgtggcagg cgaatcacgc gtatgtcgtg agcgaagtat
    16201 ttaccaccaa actgcgcacc cagaccgaga ttttgcgctt cgatcagcaa ttctttttcc
    16261 agttccacat cgcggaatgc ctgaccgtgc tcattccctt ccgttggcag ttcgtcatag
    16321 tatttagcgg aagccagttt cactgtttta aggttagttt ctgcagaagt tccaccaatt
    16381 acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc
    16441 aggtaatttt tcagtttccc cggcgttagt aacgctttgg tttcctgata gagatacgtc
    16501 ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtactcgtc gccatcaacg
    16561 gcataaagat cgatctgcgc tggcagattg gtgccggtat tcacctcttt atacatatcc
    16621 agcggcgcgt tttgcgagta gcgcaaatta tcttcgatat aagtgttata gacaccgcgc
    16681 gccagcgccg cttcatcaca gccaccggtc catacacgct gccctttttt accaacaata
    16741 atcgcagtgc cggtatcctg acaggttggc agaacacctt tcgccgcgat gtcggagttg
    16801 cgcaggaatt gcagcgccac atatttatca ttttcgctgg cttccgggtc acgcagaatg
    16861 tcggccacct gttgttggtg agccggacgc agcataaatg acgcatcatg aaacgcctga
    16921 cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca
    16981 gatacgctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga
    17041 aaaggagcct gataatgaaa gggtttgttt gacattgttc tctcacttac tgcctggttt
    17101 ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac
    17161 acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta
    17221 ggattcttgc tttaatagtg ggattaattt ccacattaaa acagggattg atcatgcaaa
    17281 aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt
    17341 atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga
    17401 aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg
    17461 agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt
    17521 tcctgttcaa agtattatgc gcagcacagc cgctctccat tcaggttcat ccaaacaaac
    17581 gcaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg
    17641 agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc
    17701 ttgcgatgaa cgcgtttcgt gagttttccg agattgtctc cctactccag ccagtcgcag
    17761 gtgcacatcc ggcaattgct cactttttac aacagcctaa tgccgaacgt ttaagcgaac
    17821 tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa
    17881 aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat
    17941 tttacccgga agacagtggc ctgttctccc cgctgttact gaatgtggtg aaattgaatc
    18001 ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc
    18061 tggaagtgat ggcgaactcc gataacgtgc tgcgtgcggg tctgacgcct aagtacattg
    18121 atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga
    18181 cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct
    18241 tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt
    18301 tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac
    18361 cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc
    18421 gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct
    18481 aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg
    18541 ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc
    18601 tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc
    18661 gcgcaggcga acgcgcaact caaactgaca gctcctgaat ccaacctgga agtgagttat
    18721 caaaactatc atcgcggcgt attcagcagc cagttgcaac tgttggtgaa acccattgcc
    18781 gggaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat
    18841 catggtccct tcccgcttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca
    18901 attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt
    18961 gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt
    19021 tcgctcaagc cactgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag
    19081 ttccagttaa atgctgacag agacggcaaa gccatctccc tttccgggga ggcgcaaagt
    19141 ggtcggatag acgcagttaa cgaatacaac cagaaagtgc agttgacctt taataatctg
    19201 aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg
    19261 tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg
    19321 gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat
    19381 tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta
    19441 aaagtcggcc agattgatgg tgaagcctgg catcagttta gccagcaata taacgcgcaa
    19501 actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa
    19561 gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact
    19621 atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc
    19681 ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt
    19741 tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac tgagtttatg
    19801 actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag
    19861 caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat
    19921 accatcacca ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg
    19981 ccactggaag atttcgttgg tatgtttgcg atgccagctc ttaacgttcc ggctgtaccg
    20041 gcaattccgc agcagtaatt cacggccaaa acgccggaga attttcactt cggcgttttc
    20101 atctgaaaac aaaccagaga ttgcctgatg cgctacgctt atcaggccta ccttttcctg
    20161 caacactttg aatttatgag tttttgtagg ctggataagg cgtttacacc gcatccggca
    20221 taaaaaacgc gcactttgtc aacaatctga aacgccggag gtttttctct ccggcgttat
    20281 ttctgacttc agcataaagt catactgaat actcattttc acatcccagt aattcaggcg
    20341 atcgcccagc gtactgccat cgcgcttttc ccgcccttta tcggcagcaa gaaagaaatt
    20401 catcccttgt aacggccctt cgctgacccg atagttcata ccacctaaga tacccgcgcc
    20461 ttcaatccgc acatctcgtt tactggattc atagccgcga gtcacaatct gtgcaaatgt
    20521 cagtgtaaat tgcggcgtta aacgatagtt gacgcccaat tgccaggact gcattccttc
    20581 acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca ccacggcacc
    20641 attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg gcgcattcca
    20701 ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac gacttaaacc
    20761 ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg ggtcgagcgg
    20821 tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta ccgatgcccc
    20881 cagagaatag cgactggtat catcatgcca actggcggct aaaaaagaac gtgcgcgatg
    20941 tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac cggttgttga
    21001 atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat ttggtgttaa
    21061 agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg aactgccagc
    21121 cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga agcgacctga
    21181 tttaagcagg ctattttcac tgggttttat ttttactgcc cccagggcaa agccaaaact
    21241 tttatcacca tcaagatacc accgggtact catatcagca cgagcaccta atttataaac
    21301 ataatatgcc ccgccttcaa cgccaataat attggaataa taaccactat tgaaatcgag
    21361 caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg gcttatccgc
    21421 cctgcgcaat tcgtttttca gtctgatgcg tagtcccgcc tcatcgtcag ccagttgcgc
    21481 cgcataagca gagataaggc cagaggcagc cgtcaggcaa ataacggcca tggccactat
    21541 ttttctcata gttgcacctt tgatgttctg attattttta ttgattatta attagtgata
    21601 tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac gatttctttg
    21661 aattttttat ccgtaagcgg ataaaaccag ataataacga atgccagtag cataaatccg
    21721 caaggtacta aggcaattga tgtgcggatg cccataataa cttccggcgt ttgcacctga
    21781 ttggcaatat atccgcttaa ccccaaaata aaggcaggaa ttgaacctcc gattgcctga
    21841 ccgcatttac gggtaaatga gaatagtgaa taggtgagcc cttcaattcg cacgccggtc
    21901 aggtattcac cgtattctac ggtatcagct tccagtgccc acatcactgt catagtaacg
    21961 ccctgaccaa ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagacggaa
    22021 acccagaaga atagcagata accgcaggtt cccagcaaag cgccaatcag gaaggtattc
    22081 tttttaccga tcctcgcgac catccccggc acaagcggtg ccgatgccac agtaccaacc
    22141 aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac atagcgcacg
    22201 tagaacaacg acgaggcgct gaccgcaaag gtcgaaatca gcacacacag cgcaccgatg
    22261 cacaacataa acagcgggcg attccgtttc agggtttgca aactgatctt caatgacggc
    22321 tgcgcaacga tacgtaccac attctcacgc gtcgatttga agcagatgaa gtaaagcacc
    22381 attccggcaa tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg
    22441 ctggagttct taatgctcgg ccctatcaga aatgccaggc agacaaaggt caatgaagcg
    22501 gcaatcccac gagccgcgcc cagacgggcg cgggattgtg gttgttgggt catcgcggta
    22561 gcaagtgaac cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc
    22621 aaatatgcat acactacttt gcggctatgg ctccagtcgg tcggtaccca gaataccagc
    22681 acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa ttttccccag
    22741 cgggtattca cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc
    22801 cgcaccagta acagcatggt gcccgccgca gcggcaccga cgccagcgac gtcggtgtag
    22861 taactcaaca ggaagagcac ccccattgcg aaggcgaagt tattggcgac gtcaccgagg
    22921 ctgtagccga cgatggtgcg ccaggagagt tgttgattca ttgtttgcct ccctgctgcg
    22981 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
    23041 gtttgcggtc gcgggtgaag atccccttct tgttaccgcc aacgcgcaat atgccttgcg
    23101 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
    23161 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
    23221 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
    23281 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
    23341 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
    23401 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
    23461 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
    23521 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
    23581 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
    23641 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
    23701 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
    23761 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
    23821 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
    23881 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
    23941 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
    24001 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
    24061 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
    24121 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
    24181 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
    24241 agagcattac gctgcgatgg atcccggcat agttaaagaa atcatggaag taagactgct
    24301 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
    24361 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
    24421 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
    24481 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
    24541 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
    24601 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
    24661 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
    24721 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct
    24781 cattaagata aaaatactgg tcaaccttta atctgattag ttaaccaatt ctgtgttgca
    24841 cacagttatc tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg
    24901 atcaaaaaac caaaaactgc aatacccaca gcgtgatatt gatcgcatta atctttaaaa
    24961 cggcgttgac agaagccata aaataagcaa ccgaacgttc gttcgagcat attgggaacg
    25021 ataaactctc atgacatatg gctacggtga atatttttgg gggggaattc tccgatcccg
    25081 gctagattgt attttgtaca gggaaattaa agtatgaaac gcgggcacag ttatcaccag
    25141 cccgcgaaat gagaggatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc
    25201 tgagcaaacg acgcttcacg cgccagatct tccgcttcga tatcggccag tacaccgtag
    25261 gtcagatcca gcagtaaacg tgaagtcatt gacgtgttga tgtctgggct tatctcgcct
    25321 ttttgctggg catcacgcat ccgctgggca acaaattccg tgatcgtcat atgcttattt
    25381 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc
    25441 gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca
    25501 aaatgaatcc ctttaatcgg ttcccggaaa cgagccagcg ccctctcctg gtcctgtaag
    25561 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
    25621 atggcgcaag atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
    25681 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
    25741 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
    25801 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatactt ttcactctga
    25861 aattggttaa ccaatagccg tgatttggct gccagttatt tagcagagac gttgatgagt
    25921 ttatcccggt aaacaacacg cacggttgtt taccggtccg ttagtgtatt aattgagctc
    25981 ctgtacccca ccaccggaga cggtgagaat ttgtccgctt acccagctcg cagcaggcga
    26041 gcaaaggaac agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt
    26101 gtgctgtaac attttttgtt caatttctgg tgtaataacg gatttcaggg catcggttaa
    26161 tattgccccc ggcgcaatgc catttacccg aatatttttt tcacccaggt caaacgccat
    26221 atttctgacc agatgactgg ctgcagcttt agatgatgca taggaagtca tgtttatatt
    26281 tttattttct gccgccatag aagtgatggt cagaataacg ccaccgccat ttttttccat
    26341 ttctggcgca acaagttgtg acagatggaa aaaagaaaac acattcagtt cataagcacg
    26401 gcgaaaatcc gccattggca tatcaaacgg tttaggtcca ccgccaccgg cgttgttaac
    26461 cagaatatca actttaccca gcttactgat agcaaagtct gccagtgcag agagttcctg
    26521 ttcggaagta atatcacaac ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc
    26581 tacaacatgg ttagctgcgt cggcgttaat atcactgacc accacagatg cgccagctgt
    26641 cgcgaatgta atggcgattt ctttaccaat acctgcaccc gcacctgtga tgatggcgca
    26701 tggcgcatgg cgcatggcgc atggcgcatg gcgcatggcg catggcgcat ggcgcatttt
    26761 ccgtcg
//
LOCUS       DAJVYM010000043        25777 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVYM010000043 DAJVYM010000000
VERSION     DAJVYM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.043"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..830)
                     /gene="dgt"
                     /locus_tag="OQS60_001695"
     CDS             complement(<1..830)
                     /gene="dgt"
                     /locus_tag="OQS60_001695"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS2387475.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
     gene            914..1612
                     /gene="mtnN"
                     /locus_tag="OQS60_001696"
     CDS             914..1612
                     /gene="mtnN"
                     /locus_tag="OQS60_001696"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS2387476.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1605..2405
                     /gene="btuF"
                     /locus_tag="OQS60_001697"
     CDS             1605..2405
                     /gene="btuF"
                     /locus_tag="OQS60_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS2387477.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2443..3066
                     /locus_tag="OQS60_001698"
     CDS             2443..3066
                     /locus_tag="OQS60_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS2387478.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3113..3457)
                     /gene="erpA"
                     /locus_tag="OQS60_001699"
     CDS             complement(3113..3457)
                     /gene="erpA"
                     /locus_tag="OQS60_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS2387479.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3450..3515)
                     /locus_tag="OQS60_001700"
     CDS             complement(3450..3515)
                     /locus_tag="OQS60_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387480.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3539..4960)
                     /gene="clcA"
                     /locus_tag="OQS60_001701"
     CDS             complement(3539..4960)
                     /gene="clcA"
                     /locus_tag="OQS60_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS2387481.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5185..6465
                     /gene="hemL"
                     /locus_tag="OQS60_001702"
     CDS             5185..6465
                     /gene="hemL"
                     /locus_tag="OQS60_001702"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS2387482.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6623..8605)
                     /gene="fhuB"
                     /locus_tag="OQS60_001703"
     CDS             complement(6623..8605)
                     /gene="fhuB"
                     /locus_tag="OQS60_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS2387483.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8602..9492)
                     /gene="fhuD"
                     /locus_tag="OQS60_001704"
     CDS             complement(8602..9492)
                     /gene="fhuD"
                     /locus_tag="OQS60_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS2387484.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9492..10289)
                     /gene="fhuC"
                     /locus_tag="OQS60_001705"
     CDS             complement(9492..10289)
                     /gene="fhuC"
                     /locus_tag="OQS60_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS2387485.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10340..12529)
                     /gene="fhuA"
                     /locus_tag="OQS60_001706"
     CDS             complement(10340..12529)
                     /gene="fhuA"
                     /locus_tag="OQS60_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS2387486.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12749..15283)
                     /gene="mrcB"
                     /locus_tag="OQS60_001707"
     CDS             complement(12749..15283)
                     /gene="mrcB"
                     /locus_tag="OQS60_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS2387487.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15479..17908)
                     /gene="hrpB"
                     /locus_tag="OQS60_001708"
     CDS             complement(15479..17908)
                     /gene="hrpB"
                     /locus_tag="OQS60_001708"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS2387488.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17982..18512
                     /gene="thpR"
                     /locus_tag="OQS60_001709"
     CDS             17982..18512
                     /gene="thpR"
                     /locus_tag="OQS60_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS2387489.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18527..19231
                     /gene="sfsA"
                     /locus_tag="OQS60_001710"
     CDS             18527..19231
                     /gene="sfsA"
                     /locus_tag="OQS60_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS2387490.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19409..19864
                     /gene="dksA"
                     /locus_tag="OQS60_001711"
     CDS             19409..19864
                     /gene="dksA"
                     /locus_tag="OQS60_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS2387491.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19901..20827
                     /gene="gluQRS"
                     /locus_tag="OQS60_001712"
     CDS             19901..20827
                     /gene="gluQRS"
                     /locus_tag="OQS60_001712"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS2387492.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20866..22284
                     /gene="pcnB"
                     /locus_tag="OQS60_001713"
     CDS             20866..22284
                     /gene="pcnB"
                     /locus_tag="OQS60_001713"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS2387493.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22281..22760
                     /gene="folK"
                     /locus_tag="OQS60_001714"
     CDS             22281..22760
                     /gene="folK"
                     /locus_tag="OQS60_001714"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS2387494.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23130..23714
                     /locus_tag="OQS60_001715"
     CDS             23130..23714
                     /locus_tag="OQS60_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2387495.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23819..24559
                     /locus_tag="OQS60_001716"
     CDS             23819..24559
                     /locus_tag="OQS60_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS2387496.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24594..25777
                     /gene="htrE"
                     /locus_tag="OQS60_001717"
                     /pseudo
     CDS             24594..25777
                     /gene="htrE"
                     /locus_tag="OQS60_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6331 a         6984 c         6419 g         6043 t
ORIGIN      
        1 aggtctgcca cacaatagga gatgtcgtcg gcagcttcca taatccacgt taatggaaaa
       61 cgactgtaaa gcgccaaatt aagttcttta cgcaaccggg caatataggc ttcttcagaa
      121 agataataac ccggcttttt cattaaatag tgatgtgtct caggcgtttc gccacgccac
      181 cacgccggac gggtatattt taaaataccg ccaacctgtg cccaagtgag attcatccgc
      241 atcaatgtat gcaccagacg aatgccttgt gcattcccct caaaatgaca taagtcctga
      301 cgaatcttgc gccgcagctc gttaagctgt tcttccccgt cccgtaaacg tagtgccgcc
      361 acgctgcagc gatcgtcagt cagaggctgg ctttcggcat cttccgggtg caaacgttgg
      421 cgaaaccagt catttatcgc tgcttcgcca aaatgaccaa acggcggatt gccgatatcg
      481 tgcatcaggc atgacatctc aacaatgctt tcaaaggggc cggtcagttc atccaggccg
      541 tatgcttcca gcaatttaag ctctttcaga cggcttaaaa tttctttggc gatgtagcgt
      601 cccacctgct ggacttccat cgagtgggta agacgcgtgc gcacggcggc attgcgctcc
      661 agtggaaaaa cctgggtctt ttgttgcaga cgacgaattg ccggagagtt gatgatacgc
      721 ccgcgatcgc tctcgaagat ccgcaggatc tcatgttcgg ttttaacgcc ctgcggtgaa
      781 cggtaacgac gatgccagtt tatttttttt cggaaatcaa tctgtgccat gtcctctccc
      841 gcgtgagaaa tacgcttccc cgtaagcgca tggtaaacta tgctttcaaa tcgggcttat
      901 cgcgagtaaa tctatgaaaa tcggcatcat tggtgcaatg gaagaagaag ttacgctgct
      961 gcgtgacaaa atcgaaaacc gtcaaactat cagtctcggc ggttgcgaaa tctataccgg
     1021 ccaactgaat ggaaccgagg ttgcgcttct gaaatcgggc atcggtaaag tcgctgcggc
     1081 gctgggtgcc actctgctgt tggaacactg caagccagat gtgattatta acaccggttc
     1141 tgccggtggc ctggcaccaa cgctgaaagt gggcgatatc gttgtctcgg acgaagcgcg
     1201 ttatcacgac gcggatgtca cggcatttgg ttatgaatac ggtcagttac caggctgtcc
     1261 ggcaggcttt aaagctgacg ataaactgat cgctgccgct gaggcctgca ttgccgaact
     1321 gaatcttaac gctgtacgtg gcctgattgt tagcggcgac gctttcatca acggttctgt
     1381 tggtctggcg aaaatccgcc acaacttccc acaggccatt gctgtagaga tggaagcgac
     1441 ggcaatcgcc catgtctgcc acaatttcaa cgtcccgttt gttgtcgtac gcgccatctc
     1501 cgacgtggcc gatcaacagt ctcatcttag cttcgatgag ttcctggctg ttgccgctaa
     1561 acagtccagc ctgatggttg agtcactggt gcagaaactg gcacatggct aagtcactgt
     1621 tcagggcgct ggtcgccctg tcttttcttg cgccactgtg gctcaacgcc gcgccgcgcg
     1681 tcatcacgct ttctcccgcc aacactgaac ttgcctttgc cgccgggatc acgccggttg
     1741 gggtcagcag ctattccgac tatcctccac aagcgcaaaa gattgagcag gtttccacct
     1801 ggcaggggat gaatctggaa cgcattgtcg cgctgaaacc cgatctggtg attgcctggc
     1861 gtggaggtaa tgccgagcgg caggttgacc agctggcttc gctgggaata aaagtgatgt
     1921 gggtcgatgc gacaagcatt gaacaaattg ccaatgcgtt acgtcaattg gccccctgga
     1981 gtccgcaacc ggataaggcc gaacaagccg cgcaatccct gctggatcag tacgcgcaat
     2041 tgaaagcgca atatgctgat aaacctaaaa aacgtgtttt tctgcaattc ggcattaatc
     2101 cgccatttac cagtggaaaa gagtcgattc aaaaccaggt actcgaagtt tgtggcggag
     2161 aaaacatctt taaagacagc cgggttccct ggccgcaagt tagccgcgaa caggtgttag
     2221 cacgctcgcc acaggcgatt gtcattacag gcggaccgga ccaaattcct aaaatcaaac
     2281 aatactgggg tgaacagctc aaaattcccg ttattcctct cacgagtgac tggtttgaac
     2341 gtgcaagccc acgtattatc ctcgctgcac aacagctctg taatgcgctt tcacaggtag
     2401 attagcgcct gaccttttta ggtgctcagt ggggattgaa caatgctcgt ctattggctg
     2461 gatatagtcg gcacagcggt atttgccatc tccggcgttt tgttagccgg aaaattgcgt
     2521 atggaccctt ttggtgttct ggtactgggc gtggttaccg ctgtaggcgg cgggacaatt
     2581 cgcgacatgg cgctggatca cggcccggta ttttgggtga aagatcccac cgatctggtc
     2641 gttgcaatgg taaccagcat gctgaccatc gtgctggtgc gccagccaag acgcttacca
     2701 aaatggatgt tgccggtgct ggacgccgtt ggtctggcgg tgtttgtcgg cattggcgtg
     2761 aataaagcct ttaatgcgga agtcggtccg ttaatcgcgg tttgtatggt cgtcattact
     2821 ggcgttggcg gcgggatcat tcgtgatgta ctggcccgcg agatccccat gattttacgt
     2881 acagaaatct acgcaactgc ctgtattatc ggcggtattg tccacgctac ggcttattac
     2941 acattttccg taccactgga aacagccagt atgatgggca tggtcgtgac gctattgatt
     3001 cggctggcgg ctattcgttg gcatcttaag ctaccgacgt ttgcgctgga tgagaatggg
     3061 cgttgaagag agaagatgcg ccggatagtt tatccggcac atgaacaaca gattagatgc
     3121 taaaggaaga accgcaaccg caggtgcttt tcgcgttcgg gttggtcacg atgaaacgag
     3181 aaccttccag tccttcggta tagtcaacgg aaccgccgac cagatattgc aggctcatcg
     3241 gatcaaccac caagccaacg ccctgttttt cgatggtcat atcgccttcg ttcacctgat
     3301 catcaaaggt gaaaccatac tggaagccgc tgcaaccgcc accggtgata tacacgcgta
     3361 atttcagatt cgggttatct tcgtcagcga tcaggctttt aactttgttg gctgctgcgt
     3421 cggtaaactc cagcggcagt gctacgtcat cactcatatt ttgctccaaa cgacatcggc
     3481 aattgggcaa attctaaccc aataataatc gccattatct aataccctgg tatttcgttc
     3541 aagtattctc gctggctgat gcggccttgc ttcgcgccag ttgctcagct tcctgttttg
     3601 ccagcgtgcg cgcaagaatc gccgagtata gcggtttccc gccggtaaat tgcgctaata
     3661 gtgttgcgcc aagaccggta ataatcattg gcaaaatgag ctggtagtta tcggtcatct
     3721 ccagaaccag aatgatcccc gttaacggcg cgcgaataga tgccgccagt aatgccccca
     3781 ttccggcaat agcaaacgtc cccgcctcaa ggtgatattg cggaaacagc tcaacggcaa
     3841 ccattccgaa agcggttccc agcacagtac ccagcgccag catcggggca aaaataccgc
     3901 ccggcgcgcc ggaagagaag cagagtaagg tggtaatgac ccgagcgacg aagataaaca
     3961 ccagcattcc catgctgaaa ttccccgcgg tagcgatagg aatcaggtta aaaccgccgc
     4021 ccgacgttgc tggtgccaca aaccccagca atccacacag accgccaatc gcaccgccca
     4081 tcagtaccca tttggtaata ttgccgccgt gcacacggtg cagcaaatcc tgcatcccca
     4141 gcacccattt attaaaaata gggccgaaaa tgccaaaaat aataccgagg atcagataaa
     4201 gccacagcgt attaagcggc gcatcagaaa gtttaccgac gtcaatcaac gcaacttcat
     4261 gattaaaaat ccggtacata atggtcgaca taatgacgcc aataaatacc gcttttatcg
     4321 aaattaacgt atagcgaaac tgcggacgca tctcttcgat aataaacaaa atacccgcca
     4381 gcggcgcgtt aaaggccgca gccagccccg cagcagcacc ggttgccagc agcgtatggc
     4441 gagcttcgtc acctttcagg cggaaaatat caagcaccat acggccaatg ttaccgccga
     4501 tctgcacggt tggcccttcg cgccccaaca ccatgcctcc gccgagtgtc cccagcccgc
     4561 caaagaactt caccggcaat acacgccacc agcgaacggg acgttgatct tccagcgccc
     4621 cttcaatttc cgggatcccc gaaccacctg cttccggcgc gtatttgcgc accaaaaagt
     4681 agccaaacat cgccagcacc gccgaacaga gaaaagcgac ggttaacaga agcggataat
     4741 tatcagcagt atgtaccagc gcccccatac gttggttctg caaccaggcg acacctttgt
     4801 caaaagcaac cgctgccagc ccaacaagcg tgccgacgac tgccgccata aacaaaatgg
     4861 ctaacggggt tttatcgcgc tcaagaagtt ggcgaatcag ttgtctgcgt cgcaggcgcg
     4921 cggcctgcgg tgtttctaaa gagggagtat cagttttcat ccaatgatca cttattggtc
     4981 atacaaataa gatgacggca ttttactcgc acgttccatg aaaatccttt gcaaagcgta
     5041 atgtttcaaa tgcacaaaac ttttatactc taagaaatca ggattgcacc agtacaagca
     5101 gcctgatgtt tgacgagtat ttaacttgtt atgaataaca tagaatagca gccattcact
     5161 ttttctacga atcaggaacc ctccatgagt aagtctgaaa atctttacag cgcagcgcgc
     5221 gagctgatcc ctggcggtgt gaactcccct gttcgcgcct ttactggcgt gggcggcact
     5281 ccactgttta tcgaaaaagc ggacggcgct tatctgtacg atgttgatgg caaagcctat
     5341 atcgattatg tcggttcctg ggggccgatg gtgctgggcc ataaccatcc ggcaatccgc
     5401 aatgccgtga ttgaagccgc cgagcgtggt ttaagctttg gtgcaccaac cgaaatggaa
     5461 gtgaaaatgg cgcaactggt gaccgaattg gtcccgacca tggatatggt gcgcatggtg
     5521 aactccggca ccgaggcgac gatgagcgcc atccgtctgg cccgtggttt taccggtcgc
     5581 gacaaaatca ttaaatttga agggtgttac cacggtcacg ctgactgcct gctggtgaaa
     5641 gccggttctg gcgcactcac gttaggccag ccaaactcgc cgggcgttcc ggcagatttc
     5701 gccaaacata ccttaacctg tacttataac gatctggctt ctgtacgcgc cgcgtttgag
     5761 caatacccgc aagagattgc ctgtattatc atcgagccgg tggcaggcaa tatgaactgt
     5821 gttccaccgc tgccagactt cctgccaggt ctgcgtgcgc tgtgcgacga atttggcgca
     5881 ttgctgatca tcgatgaagt aatgaccggc ttccgcgtgg cactggctgg cgcacaggat
     5941 tattacggtg tggaaccgga tctcacctgc ctgggcaaaa tcatcggcgg tggaatgccg
     6001 gtaggcgcat tcggtggtcg tcgtgatgta atggatgcgc tggccccgac gggtccggtc
     6061 tatcaggcgg gtacgctttc cggtaaccca attgcgatgg cagccggttt cgcctgtctg
     6121 aatgaagtcg cgcaaccggg cgttcacgaa acgttggatg agctgacaac acgtctggca
     6181 gaaggtctgc tggaagcggc agaagaagcc ggaattccgc tggtcgttaa ccacgttggc
     6241 ggcatgttcg gtattttctt taccgacgcc gagtccgtga cgtgctatca ggatgtgatg
     6301 gcctgtgacg tggaacgctt taagcgtttc ttccatatga tgctggacga aggtgtttac
     6361 ctggcaccgt cagcgtttga agcgggcttt atgtccgtgg cgcacagcat ggaagatatc
     6421 aataacacca tcgatgctgc acgtcgggtg tttgcgaagt tgtgatccag catggtttgt
     6481 cggatgcggc gtgaacgcct tatccgacct acgattcggg gccgtttgta ggcccgataa
     6541 gacgcgccag cgtcgcatcg ggcatccgtt gtcgcgtaaa cgctttatcc gtccaggcgt
     6601 acagggccgt tatatggaaa aattaacggc tctgctttct cagcaaatag ataaaatatg
     6661 gcgcgccgat aaaggtcgac agcagccccg ccgggatctg gaatggaaac agcaccatcc
     6721 gcccacacca gtcagcgaat accagcagta aaccacccac cagcgccgaa attacgatgt
     6781 gtggcatcgt ccgtcgaaag cccatcatcc gcgcaatatg cggtgccatt aaaccaacaa
     6841 aactcaacgg tccaatagtc atcgtcgcgg tcgccgtcag gcaagccgct aacagcagca
     6901 gcgcaattcg cgtcggcgtc agcgccattc ctacggctcg tgcggtatca ccacccagcg
     6961 gtaaaatggt cagccagcgg cggtacagcg gggtaatcgc cagcaaaatc accatcacaa
     7021 ttccggtacg ccagacctgc gcatcggtcg cgttgtaggt cgaaccggaa atccaggtca
     7081 gcacttgcgc cattcgcggg tcaccgctcg cctgcaacat catcaaaagc atggtgaatg
     7141 cggtacttaa cgccatcccc gccagtaaca tacggtgtgg ggaaaatcca ccgcggccgg
     7201 cggcgatcat aatgatcaac agcgtcaccg ccgccccgat actgcccgcg ggtaacagcc
     7261 agccaaaggc attacccggc accagaaaca gcattaacac cacgccaaac gccgcgccag
     7321 agctaatccc cagcacttcc gggcttgcca tcgggtttcc ggtcagccgc tgaataatac
     7381 agcccgccac cgccagcatg acgcccgcaa acagcgccgc cataattcgc ggccagcgcc
     7441 agggcatcaa atcctcgagc aacgccccgc tcgcccacgt ccagccgtgc gcatcacgac
     7501 caaacgacag cgccaccacc acagccatca acagcagcac gccgcccgcg agggcaaacg
     7561 ccagcacatg ttggcgttca gccgcgacac gatcgttgac cttcatatcc ggcgcgctaa
     7621 tgctgcgtaa acgcggcaac agccacagta gcagcggcgc accgatcaac gcagtgaccg
     7681 aaccggtgga cacttccatc cacacgcgag tcagccagag gatgatttga tcggaaagcc
     7741 agaggatcag cgccccaatc agcgacgcca gcatcaaccg tggcaacaaa cgccgcgccc
     7801 ccagcatttt cgccagcagc ggcgcgaaca aaccgataaa gccgataatc cccaccgcat
     7861 tcaccagcag cgcactgatg actatcgcca gcgacagcgc cgccagacgc gcgagcgaca
     7921 aggccagccc gagattgcgc gccacgccat catcaagccc catcagggtt aacgggcgaa
     7981 gtagcaataa cgtcaacatc acgccgccca gcaactgcgg ccataaacgc tcaacgccgc
     8041 cccagtcggt ttgcgtcagc gttccggtgc tccacaggaa catgctttgc agctggtcgt
     8101 gatggaagat aaccagtagc tgattaattg ccccgcaata aaggctcacc accagccccg
     8161 cgagaataag cgtaaccggc gaaagccgtt tcccccacgc gacgccaaag acgattaagc
     8221 caacgacaca agcgcccacc agtgcggcaa actggctcgc catagcacct gggatcgccc
     8281 aaagtgtggt gacggtaatt cccagttgcg cgcctgtagc aacgccaagc gtcgtcggct
     8341 ccgccagcgg gttacgcagc acttgctgaa acagcacgcc caccagcccc aggcccgcgc
     8401 ccaccagcag cgaaatcgcc agacgcggca acaagctgta gtgaaaaatc atctgctcga
     8461 tgacatcgat gtctggcgac caggcagcct gcgcccactg gctacgcggc agcgcctgcg
     8521 agaagttcat ccaggtgagc gccgtagcga caatcactaa cagcgccagc aataacgccg
     8581 gaaaaagcgc aattcgttta ctcacgcttt acctccgatg gcgttatcca gaacgcgcac
     8641 aaagtgcatt gccgagagtg tcgcaccata aaaccagact gcaggtacgc gctgaaagcg
     8701 tccggcgcgg acaaacggca tggcctgcca cagcggggtt gccattagcg catccatgtc
     8761 tttgctgttg tcgtgatcaa aacagagcac atcaacgtct ttatacgctg ccagacgatc
     8821 gatactgacg gcggtactgc cccagaagtt ggtttcccct tgccaggcat ttgggatgcc
     8881 gtactcatcg agaatttcct ggaacaagct gtttggaccg aagaccagca tatggcgcgg
     8941 atcgataagc gtcgtcagca gtaacgggcg tgcaccacgc ttcacaaagc ggggtttcat
     9001 gctgcggata aagtcttcgt attgcgctaa atgcgtttcc gctgcgcttt gcaggttaag
     9061 taaatctgcc atttccgtca gcgatttacg cgccatcgcc aacggctgtt tgccgtcact
     9121 gaagttaaat ccgcgacccg gcgcaatacg tgccagcatt tctgatgaag ggccatatcc
     9181 tgccgaccag accataaacg acggtttcat ttcggtcagc agttcaaggt taggttctgt
     9241 acgcaaaccg acgtcaatca ctgagtccgg caatggtggt tcgctgaccc acaggcgata
     9301 gttgatggta tccgccacgc cgtaaggcac gatgccgagc gccagcagta attccaccgg
     9361 caaccactcc agcgccacaa tacgattggg gtcaatagcc gccgcgtggg cggtattcat
     9421 ctgccataac aacggagaaa gcgccatcgc cgttaacagt cgacggcgcg aaataagagg
     9481 taagccgctc atcaataaac aaaactcaca ggtgcagcac ccgccggatg cggcaaaata
     9541 cccatcggga tgccataaat catttcgagg gtttcgccgc gcataatttc cgcaggcgtt
     9601 ccctgagcaa tcatttcacc actgcgcagg gcgaccagat aatcacagta gcgtgccgcc
     9661 atattgatat cgtgcaacac ggcaatgacc gtcaggccac gttcctgact taaacggtgc
     9721 accagcgcca gcacatcaac ctggtgggcg atatccagcg ccgaagtcgg ttcgtcgagc
     9781 aacagacaac ggctatcctg cgccaccagc atggcgatcc acgcccgctg acgttcgccg
     9841 ccagagagac tatcgaccag ccgatgcgcc agcggtttta agccaaccag cgagatagct
     9901 tcctcgacct tttcgcgatc tgccgcacca aagcgcccca gcgcgccatg ccacgggtaa
     9961 cgaccaatcg ccaccagttc acgcacggtc atcccttctg ccggaggaag ctgctgcggc
    10021 aaataagcca ctttgcgggc aaacgctttg ctgctccagc tttccagcgg ctgggcatca
    10081 agaagaatct ccccttctga cggaggctga tgacgaccaa gcattttgag cagagtggat
    10141 ttaccagaac cgttgtgacc aatcagaccg gtcactttcc cggcaggaaa ggttaacgac
    10201 agcggatgca aaagcgtgcg cccgggcaca cgaaaggaga tattacgcag tgcaaaagtg
    10261 gtatcggaat gattcgtgta ttcctgcata acagccaact tgtgaaatgg gcacggaaat
    10321 ccgtgcccca aaagagaaat tagaaacgga aggttgcggt tgcaacgacc tgacgttctg
    10381 cgccccagaa gcagccataa gtctggaagc aactggcaac atactcacga tcgagcaaat
    10441 tgttgacgtt cacagcaagg ctggatcccg ccataccaaa gcgtgccaga tcatatttta
    10501 caacagcatc cattacagct gtgctaccca ctttaaaagt gtttgccgga tcaccatagc
    10561 tggaaccaat aaaacgacca cctgttccca atgttaaacc agaaagcggc ccttcattga
    10621 aggtataatc cccccagaga gatgccatat gctccggtac ttgttctgga gttttacctt
    10681 tgagattggt gtctttggtg tattccgcat cggtgtaggt ataagaagcc gtcaagttaa
    10741 tattggcatt taacgccgct tttgcttcaa gttccacacc acgagcgcga atctcacctg
    10801 ctgggacttg cgctaatggg tttgttgggt ctgctgtcag gttatttgtt ttcgtcaatt
    10861 gatagactgc gcccgtaaca acgaccggca tatcattcgg aacatatttt acgccagctt
    10921 catactgttc acctttcgat ggcttatagg aaatgcgcgg gttgctccac agatcgaaag
    10981 cactcggttc aaaggactgg ctatagctaa agtaaggtga aataccatta tcgaataagt
    11041 aatttacacc accgcgccag gtgaactgat gatcattgcg ctcaatataa ccttcagtcg
    11101 gcgtataaga gttttcacga acagtggtcg cttgcttaga ccaatcgtag cgtcccccca
    11161 gagtgaatac ccatttattc cattccgcct gatcctgaac ataaatacct gtttgtttgc
    11221 tttcattcat ctggtatggc tctgcgctac caaatgcaaa gtattcaggg tgatatttgt
    11281 tataaagatc gatggatggt gcggatccaa agctggcatt gatatcatta cgcatccgca
    11341 tgaagtctac ccctgtcagc aaagtatgct cgacgtcacc tgtagcaaac ttactttcaa
    11401 gttgggtatc aacgctaaag ttttgtagac gctcattatc caccactgtc ccgcggttta
    11461 gggtatgacc atcaccagca atccctgtgc cataaacact tttctgtgag gttttcattt
    11521 cactgaaacg cagattctga cgcacggtga aggtgtcatt aaaaccatgt tcaaaactat
    11581 atcccaccat tttctggtta cgggagtatg tattattcga tgcaccttca ttgaagtcag
    11641 tcggtagacg cttaccatta ggcaatggct caacggtccc ctctttcggc aaccaaccgt
    11701 aataacccgt ttctggttca ttctggaaat aggacaggaa agtaaaattg gttttgtcgt
    11761 ctggacgcca tgagaatgat ggagcaatgg tataacgctg agattcagag tttttctgct
    11821 gttcgtttgt tgagcgagcg aggcccgtca aacggtaaga gaactcaccg ttatcatcca
    11881 gtgcatcact gaaatcgaat ccggtctgaa acagattatc cgtccccatt ttaaactgaa
    11941 tttctttcag gggttcagtg gtcggacgct tactcaccat agaaataatc ccaccaggat
    12001 tactttttcc atagagaaca gacgtcggcc cacgcatcag ttcaaccctc tcgagcatgt
    12061 aaggatcaat cacagcatcg ttataaaagt tgccctgaag ttttagtcca tcaaggtaat
    12121 tattttgatt cagaccaaca gatgagaaac cacgaatgat aacaaaatca tatgtattag
    12181 aagcaccacg actgctaacc gtaacgccag gtgtataacc taatgcttct tttacagact
    12241 gaaattgacg catctgcatc tcttcatttg tcacaaccga aatagactgc ggcgtttttt
    12301 caattggtgt atccgtttta gtcgcagtag cagagtgctt cgccgcaata gttgcagcag
    12361 gcccccatgc gctttcttgc ggcgcaggtg cagcggtaac ggtgatagtg tcttctttcg
    12421 gttcaaccgc tgcctgtgca taaacagaca tgccgctaac cgctgtggct actacaactg
    12481 cgattttacg cagtgagtgt tttggctgag cagttttgga acgcgccatt ggtatatctc
    12541 tgatgtaaag tgaatgataa cgtaaacgag aatgattatt ataaagataa gataatattc
    12601 gaaacgctgg cgggttcgca agcggtacgc aatctgagcg ttcctgaagg gttaataaac
    12661 aaccagatga aaagaaaggg ttaatacttt aggtgggatg ttattttacc ggatggcaat
    12721 tcgccatccg gtatttcacg cttagatgtt aattactacc aaacatatcc ttgatccaac
    12781 cggctacacc gtcgctgtct ttctgctctt gctgagcagg ttgctgttgc ggctgttgct
    12841 gcggctgaga agactgatca aacggattgc ctgacggctg ctgctgcatc tcgctctgct
    12901 ggcacagcga ttgcggatcg ctggtccaga ccggcaagac acgcatgcca ccgctgcaaa
    12961 caaagttgcc gtcgtagtcc acgcccatat ctgcaatatc ttctggcgga acaagattca
    13021 gcggcgttgg cgtctggtta gccagataac gctgataaat agacattgcc ccgctggcac
    13081 catacagttt ggtcggctgg ttgttatcac ggcctaccca ggtgatggtc accgtgctgc
    13141 cgtcaatgcc cgcaaaccag gtatctacgt tattgttggt agtccctgtt ttccctgcca
    13201 gatgcaggtt cgggtatttc gccccaagtt gacgacccgt accacgttgt accacctgct
    13261 gcatggtcca tagtgtcaga tacgccgcct gcgccggaac agcgcgttct gcctgcggga
    13321 agctctgata cagcaccttg ccatcttccg cgattaccga acgcagtgca gaaagcggtg
    13381 cacggttacc gccgctggcg atggtctgga atgcctgcgc cacttcgatt ggcgttaagt
    13441 tcaacgcccc cagcagcatc gccggaaccg gatgcagctg atctttcggc acgcccagtt
    13501 tgatccaggt ctccgtaact gcaggcagcc ccagcgccat ccccagattt accgtcggca
    13561 cgttcatcga acgggtcaac gcatccacca gcatcactct gccgctttcg ctataacgac
    13621 ggtcatcgtt ctgcggtgac cagacctggc cattcggctg acgcagcgcg attggcgcat
    13681 cggcaatcca cgtgttcagg cgatagattt tcggctggct taaagccgtc agataagtcg
    13741 ctggttttgc aagggaacca atcgaacgac gcgcctgcat cgcacggttg tagcccgcaa
    13801 actgcggctc agaacctccc accatcgcac gaacttcacc actaaagcgg tcgacgacca
    13861 caattgcagt ttcaagatcg ctcaacttac gctgtttctt cagtgccgga atgccttcca
    13921 cggcggcttt ttctgccgcg tcctgggcca ccgagtcaaa ggtagtgaag atcttcacgc
    13981 cggagagatc ttttacctta tcgcctaatt tcgcctgcag ctcctgacgc accagttgca
    14041 taaaggctgg ctgtggagag attaccccac cgcgcggctg tacgcccaga ggacgcgcgc
    14101 tgagcatttc atacagttca ccatcgataa tctgctgttg ttgtagcaaa cgcagcacca
    14161 gattacgtcg ctccagcgcc agtttcgggt tacgccacgg gttgtagatg gacgcccctt
    14221 tcaccatacc gactaacagc gcctgctggt cgaggctgag ctcttctacc gggcgaccaa
    14281 agtaatacaa gctcgcaagc gggaagccgc ggatttcgtt gtcgccgctc tgaccgagat
    14341 acacctcgtt catatacagc tcaagaatac ggtctttgct gtaacgcgcg tccatgatca
    14401 gcgccatgta agcttcgttc gctttacgcc agtaagaacg ctcgctggag aggaacaggt
    14461 ttttcaccag ctgttgcgtc agcgtactcg ctccctgcac cgtgcgtccg gcggtcaggt
    14521 ttgccagcac cgcacgtccg atggagtaga gactgatccc atcatgctcg tagaaatgac
    14581 ggtcttctgt cgccagcaaa gtatccacca gcaagtccgg gaaaccactg cgcggcacaa
    14641 acagacgctg ctcaccgttt ggcgaagaga tcatggtgat caggcgcgga tcaagacgga
    14701 agaaaccgaa ctgacggttg ttctccatat tgacgatcgt cgccagatga tcgccatcaa
    14761 aggtcagacg cgcacgcacc tgcccttctt tgctgtccgg gaaatcaaac ggacggcgga
    14821 tcatctcaat gctgttggcc tgcacggtaa attcgccagg acgggtcatt ttcgacacct
    14881 gacgatactg tgttgcctcc agcagcttca ccatttcgtt cttgctgatg gtcatatctg
    14941 gttcaaggtt gaccattcgg ccataaactg ccgcgggcag ttgccagacc ttgccatcaa
    15001 tacggctacg aattttttga tcgagataaa cgccgtaaat ggcgatcagc acggcaaaaa
    15061 cgatagccag ttttaacagc agccatagcc agccgcgttt gccacgaggc ttacgccctt
    15121 tgcctttgcc cttacctttg cgcggcatcg gttcttcatc ctcatagtca tcataatcgt
    15181 cgtaatcatc gtcatcttcg taacgacgac ggcttacctt ttgtttgacc ggacgcgtcg
    15241 gtttcccttt gcgtccaatt ggctcgcggt cattcccggc catgcttttt ctccgcaata
    15301 ttcaggcgca aaagcccgat tttctgttct tctgttacaa gacagaagaa gatatctctc
    15361 aaaatttgtg ctcgatcggt cccctctccc ttgagggaga gggttagggt gagggtgaac
    15421 gtgcgcgctg atgccctcac cccggccctc tcccacaggg agagggagaa aaccaacctt
    15481 acgaatactt tttcgtccgt cgcgtcggtg ccgtgtttgc cgggtcgtcc ggccagacgt
    15541 gtttgggata acgccctttc atctcttttt gcacctcacg gtacgctcct ttccagaagg
    15601 cgctcaaatc tcgtgtgatt tgtagtggcc tttgggcggg tgaaagcaac tccagcacca
    15661 gcggcacgcc cccctgggcg atagtcggat tagtggcctc accaaacatc tcctgcattc
    15721 tcaccgccag tgcgggcggg ttatcttcat gataacgaat ggcgatccgg cttcccgtcg
    15781 gcacagtgta atgcgcaggc aattcactat ccagacgttg ctgcattccc caatcaagta
    15841 atccgcgtag tgcctgataa atgtccagtg atttcaggcc gcgtagtgaa tgtacgcctg
    15901 tcatatgtgg cagcagccac gtttccagcg ctgccaataa actttcatca tcaaccgctg
    15961 gccagtcata ttccggcaac cactttgcgg cgcataacaa acgcaagcgt agctgttccg
    16021 cttccgccgt ccagttgagc acgcttaaac ctttatcacg gatgccatta agcatcgcct
    16081 gatgcaactc gtcttctgac ggtttcgcca gcggctgcac tttcaccgtc aactgaccga
    16141 tttgtagccg acgccaggct ttcagcgtac cttgcgcgtc atcccattcc acggtgtcag
    16201 attgctgcac cagctgcgga cagcgttgta ctaattcatc aatatcgacc ggtagcgcca
    16261 gtaaaatccg cgcatccggc gaggcgctgc cctgcaataa taacggtgcg atcaaccatt
    16321 cgtggcggct tagcgcgtcg tcggcatcga gcatcgctcc catgccgttt gccagttgat
    16381 agcgtccatc ttgcccacgg cgatgagcaa tgcgatcggc aaacgccccg gcaagtagcg
    16441 gcgcgataag cgaactgtct gcctcaccgc cgcgtacgtt taagcgtttt aacagttgct
    16501 gactacgttg ctgccaggct ggttgattgc gcgaaaacgc cacgcccagg tcactattgc
    16561 ccatcagtgg cggctcttcg agaatggcgg caatttttgc cgcggtagca gcttcgtcgt
    16621 cgttcttcgc gctaaccagc attgccgcta aacgcggatc gttacccagc gctgccattt
    16681 tttgcccttg cgcactaagc cgttcaccgt ccagcgcccc cagcatttgt aacagacgtt
    16741 tcgcggccat tagattcact actggcggtt gatccagcca gctcatctgc gccggatcgc
    16801 tgcatcccca ttgcagtaat tccatcagca aaccggaaag atcgctttgt aagatctccg
    16861 gttcactttg cgcggcagcg cgttctgctt gttctttggc gattaaatgc aggcagatac
    16921 ccggctccag acgcccggcg cgcccggcac gctgcgtcat ggatgcctgg ctaacgcgtt
    16981 gagtaatcag tcgcgtaagc cccgtgcgcg gctcaaaacg cgccacacgc tcctgggcac
    17041 aatccaccac cagacgaata ccttcaatgg ttaaactggt ttcagcaata ttggtcgcca
    17101 gcaccacttt gcgcatccct tgcggtgccg ggaggatcgc ttttcgctga tcgttcagcg
    17161 acaacgcgcc atacagcggg cagagcaaca catcactgcc gatgcgcgaa gccagttgtt
    17221 cctgcacacg ctgaatttct ccgacgccag gtaaaaataa cagtaatgat ccgctttcct
    17281 gacgcagcat ttcggcggtg gctaccgcaa cggcttcgtc aaaacgctga tgcgcgggca
    17341 gcggtaaata atggcgttca accggaaacg agcgcccttc tgagatgacg acgggcgctt
    17401 ctggcagcat ttgctgcaag cggtcgttat ccagcgtagc cgacataatc agcagtttaa
    17461 ggtcatcacg cagaccttgt tgcacatcga gtaacagcgc caacgccaaa tccgcctgca
    17521 agctgcgctc atgaaattca tcaaggatca ccaatccaac accgctcaac tccgggtcac
    17581 gctggatcat ccgcgtcagc acgccttcgg taactacttc cagacgggta tttggcccga
    17641 cgcagttttg cgcacgcatc cggtagccaa cggtatcgcc tggcttttcg ttaagcagct
    17701 ccgccagccg ttgcgcgacg ttacgtgccg ccagacgacg cggctccagc aggataattt
    17761 tcccgttaat gccgggatgc gccagcagtt gcagcggcag ccaggttgat ttcccggccc
    17821 cggtcggcgc acttaataat acctgcggcg cacaatcgag agcggtaagt aattcaggta
    17881 agacggcagc aacgggcaac gacgacacaa aacgctccag agggttaaca ttcttcgcgc
    17941 cacattgtag catcgcggta attcataacc gagtgcctca catgtctgaa ccgcaacgtc
    18001 tgttctttgc tatcgactta cctgcagaaa tccgcgaaca gattatccac tggcgcgcca
    18061 cacacttccc acctgaggcg ggacgtccgg tcgccgccga taatttgcat ctgactctgg
    18121 catttttagg cgaagtgagc gccgagaaag agaaggcgct ttctctttta gccggacgga
    18181 ttcgtcaacc tggtttcaca ctcacgcttg atgacgccgg acaatggctg cgttcgcgtg
    18241 tggtgtggtt agggatgcgt cagccgccac gcggcttaat ccagctggcg aatatgctcc
    18301 gttcacaggc tgcccgcagc ggttgttttc aaagcaatcg tccgtttcat ccacatatta
    18361 ccttattgcg cgacgccagc gaggcggtga caatcccgcc gccaggtttt aactggtctt
    18421 atgcggtgac ggagttcacc ctttacgcct cctcgtttgc ccgtggacgc acacgctaca
    18481 cgccgctaaa acgctgggcg ctaacgcaat aacaaggatt gtcgcaatgg aattctctcc
    18541 ccctctacag cgcgcgacgc taattcagcg ttacaaacgt tttttagccg atgtgatcac
    18601 acccgatggt cgcgaattaa cgctacactg cccgaatacg ggtgcgatga ccggttgtgc
    18661 aacgcgtggc gataccgtct ggtattcgac ttcagacaac accaaacgga aatacccaca
    18721 cacctgggaa ctaactcaaa gccagagcgg cgcatttatt tgcgtcaata cgctttgggc
    18781 taacaggttg acgaaagagg ctatccttaa tgaatcaatt tcagaactgt caggctatag
    18841 ctcgctgaaa agcgaagtaa aatacggcgc agaacgcagc cgtattgact ttatgttgca
    18901 ggcggattcg cgtccagact gctatattga agtgaaatcg gttacgttag cggagaacga
    18961 acagggatat tttcccgatg cggtcactga acgaggtcag aaacaccttc gggagttgat
    19021 gaacgtagcg gctgaaggcc agcgtgcggt tatctttttc gccgtgctgc attcagccat
    19081 tacacggttt tcacccgcgc gccacatcga tgagaaatac gcgcaactat tgtcagaagc
    19141 tcaacagagg ggggtagaaa ttctggctta caaagcggaa atttctgctg aaggcatggc
    19201 tcttaaaaaa tcactgtcgg ttacattgta gtaaagtaag taactggtta atttacattc
    19261 tggtcgcgtg cgcaaatacg cttttcctca cacagttgtc aagtgttacg tttagataat
    19321 tgctatccgg aaaagcatct gctatttata gcggcctcat ttttcccccg aacatgggga
    19381 tcgatagtgc gtgttaagga gaagcaacat gcaagaaggg caaaaccgta aaacatcgtc
    19441 cctgagtatt ctcgccatcg ctggggtgga accatatcag gagaagccgg gcgaagagta
    19501 tatgaatgaa gcccagctgg cgcacttccg tcgtattctg gaagcatggc gtaatcaact
    19561 cagggatgaa gtcgatcgca ccgttacaca tatgcaggat gaagcagcca acttcccgga
    19621 cccggtagac cgtgcagccc aggaagaaga gttcagcctc gaactgcgta accgcgatcg
    19681 cgagcgtaag ctgatcaaaa agatcgagaa gacgctgaaa aaagtggaag acgaagattt
    19741 cggctactgc gaatcctgcg gtgttgaaat tggtattcgc cgtctggaag cgcgcccgac
    19801 agccgatctg tgcatcgact gcaaaacgct ggctgaaatt cgcgaaaaac agatggctgg
    19861 ctaattacag ccgttccatc acgtttacca caggcgggaa atgctcccgc cttatttttt
    19921 gttcaaagag atgacagaca cacagtatat tggccgcttc gccccctctc cttccggcga
    19981 gcttcatttt ggctctctga tcgccgcgct cggcagctat ttgcaggctc gcgcccggca
    20041 aggtcgctgg ctggtacgca tagaagatat cgacccgcct cgtgaagttc ccggtgccgc
    20101 agaaactatc ctgcgccagc tggaacatta cggtctgcac tgggacggcg atgttctctg
    20161 gcaatcgcaa cgtcacgacg cctatcgtga agcactcgcc tggttacatg aacaaggact
    20221 aagttattac tgcacctgta cgcgtgcgcg tattcaaagc attggcggta tttacgacgg
    20281 tcattgccgg gtgttgcatc atggaccaga caatgccgca gtgcgtatcc gccagcagca
    20341 tccggtcacg caatttactg accagctgcg cggcattatt cacgccgacg aaaaactggc
    20401 acgggaagat tttatcattc atcgccgtga tgggttgttc gcctacaacc tggctgttgt
    20461 ggttgatgat catttccagg gcgttacaga aatagtgcgt ggggctgatc tgattgaacc
    20521 aacagtaagg caaatctcgc tgtaccagct ttttggctgg aaagtgccag attacattca
    20581 tctgccgctg gcgcttaatc cacaaggcgc taaactttcc aagcagaatc atgcgcctgc
    20641 gttgccgaaa ggcgatccac gtccggtact aatcgcggca cttcaatttc tgggtcagca
    20701 ggcagaagca cactggcagg atttcagcgt cgaacaaatc cttcagtcag cagtcaaaaa
    20761 ctggacgctg accgccgtgc ccgagtcggc aattgtaaat tcaacattct caaatgcgtc
    20821 atgctgagct atgattagcc gctatttttt tgtcctgaat gatgtttgac actaccgagg
    20881 tgtactattt ttacccgagt cgctaatttt tgccgcaagg tgctaagccg cgaggaaagc
    20941 gaggctgaac aggcagtcgc ccgtccacag gtgacggtga tcccgcgtga gcagcatgct
    21001 atttcccgca aagatatcag tgaaaatgcc ctgaaggtaa tgtacaggct caacaaagcg
    21061 ggatacgaag cctggctggt tggcggcggc gtgcgcgacc tgttacttgg caaaaagccg
    21121 aaagattttg acgtaaccac taacgccacg cctgagcagg tgcgcaaact gttccgtaac
    21181 tgccgcctgg tgggtcgccg tttccgtctg gctcatgtaa tgtttggccc ggagattatc
    21241 gaagttgcga ccttccgtgg acaccacgaa ggtaacgtca gcgaccgcac gacctcccaa
    21301 cgcgggcaaa acggcatgtt gctgcgcgac aacattttcg gctccatcga agaagacgcc
    21361 cagcgccgcg atttcactat caacagcctc tattacagcg tagcggattt taccgtccgt
    21421 gattacgttg gcggtatgaa ggatctgaag gacggcgtta tccgtctgat tggtaacccg
    21481 gaaacgcgct accgtgaaga tccggtacgg atgctgcgcg cggtacgttt tgccgccaaa
    21541 ttgggtatgc gcatcagccc ggaaaccgca gaaccgatcc ctcgcctcgc taccctgctg
    21601 aacgatatcc caccggcacg cctgtttgaa gaatcgctta aactgctaca agcgggctac
    21661 ggttacgaaa cctataagct gttgtgtgaa tatcatctgt tccagccgct gttcccgacc
    21721 attacccgct acttcacgga aaatggcgac agcccgatgg agcggatcat tgaacaggtg
    21781 ctgaagaata ccgatacgcg tatccataac gatatgcgcg tgaacccggc gttcttgttt
    21841 gccgccatgt tctggtaccc actgctggag acggcacaga agatcgccca ggaaagcggc
    21901 ctgacctatc acgacgcttt cgcgctggcg atgaacgacg tgctggacga agcctgccgt
    21961 tcactggcaa tcccgaaacg cctgacgacg ttaacccgcg atatctggca gttgcagttg
    22021 cgtatgtccc gacgtcaggg taaacgcgca tggaaactgc tggagcatcc taagttccgt
    22081 gcggcttatg acctgttggc cttgcgagct gaagttgaac gtaacgctga actgcagcgt
    22141 ctggtgaaat ggtggggtga gttccaggtt tccgcgccac cagatcaaaa agggatgctt
    22201 aacgagttgg atgaagagcc gtcaccgcgt cgccgtactc gtcgtccacg caaacgcgca
    22261 ccgcgtcgtg agggtaccgc atgacagtgg cgtatattgc cataggcagc aatctggcct
    22321 ctccgctgga gcaggtcaat gctgccctga aagcattagg cgatatccct gaaagccaca
    22381 ttcttgccgt ttcttcgttt taccgcactc caccgctggg gccgcaagat caacccgatt
    22441 acctcaacgc cgccgtggcg cttgaaactt cgctcgcccc ggaagagtta ctcaaccata
    22501 cacagcgcat agaactgcaa cagggtcgtg ttcgtaaagc tgaacgctgg ggaccacgca
    22561 cgctggatct cgatatcatg ctgtttggta atgaagtgat aaataccgaa cgcctgaccg
    22621 ttccgcacta cgatatgaag aatcgtggat ttatgctgtg gccgctgttt gagattgcac
    22681 cggagctggc gtttcctgat ggggagacgt tgcgtgaagt cttacataca agagcatttg
    22741 acgaattaag caaatggtaa tttatttata ttacgaatat aaatattttc tgaaaatcat
    22801 acatcctctc tttccttaat aaaaatttta gtttaaacat ttttgcagaa acataatata
    22861 aattcccgca gataaatatc aatcccatcg ccatacccta accaattgca cttgtgaatt
    22921 tatttttgct taattttgaa ttaagaaaat ttaatttgtt caaaagccct taaagtgcac
    22981 aataggcgtc ctaatgaaca atggttgtaa attttggtaa caaatatcat tgctcattaa
    23041 tatctagcat ctgcttataa catttgtgtc atgaatagtc attcttcaaa ccagtagtct
    23101 gactaatgga attttattca aggattttta tgtctaaaaa attaggtttt gccctgtctg
    23161 gtcttatgct ggcaatggtt gcaggtactg cttctgctga tatggatggt ggtcagttaa
    23221 atatcagtgg tctggttgtt gataacacct gtgaaacccg cgttgacggc ggtaataaag
    23281 atggtctgat cctgctgcaa actgcaactg ttggtgaaat cgatgctggc gtgctgaatg
    23341 acaccgttgg cgctaaagcc aaacctttca gcatcaccgt tgactgcagc aaagcaaatc
    23401 cgaatccagg tagtacagct aaaatgacct tcggttctgt tttctttggt aatagcaaag
    23461 gaacgctgaa caacgacatg tctattaaca acccgtctga cggcgtgaat atcgctctgc
    23521 acaatattga tggttccact atcaaacagg ttcaaattaa caaccctggc gatgtgtata
    23581 ctaaagccct ggatgcaacg acaaaatctg ctgtttatga ttttaaagcg tcttacgttc
    23641 gtgccgtcgc agaccaaaca gcaactgctg gttatgtaaa aactaacact gcatacacca
    23701 ttacttatca gtaagtattc accaattaaa ttgattgaat gaatatacag ggaatagtgt
    23761 taataatttc tatttttata ttattccctg ttttaattaa ctctgtcagg gatggtttat
    23821 gttttttaac accaaacata caacagcttt atgcttcgta acctgtatgg cttttagttc
    23881 atcgtctatt gcggacattg tcatttcggg tactcgcgta atatataaaa gcgatcaaaa
    23941 aagtgtcaac gtacgtctgg aaaataaagg gaataacccg ttgcttgtcc agagttggtt
    24001 agatactggc gataacaacg ctgagcctgg cagtattaca gtccctttta ctgccacgcc
    24061 gccagtatcg cgtattgatg ccaaacgtgg gcaaacaatc aaattaatgt acacagccag
    24121 cacctcactg cctaaagaca gagagagcgt gttctggttt aacgtactgg aagttccacc
    24181 aaaaccagat gcagaaaagg tcgcgaatca aagtctgctg caactggcat ttcgcacacg
    24241 tataaaactt ttctatcgcc cggatggatt gaagggaaat ccctctgaag ccccgttagc
    24301 cctgaagtgg ttctggtcag gttcagaagg taaggcgtca ttgcgagtga ccaatccaac
    24361 cccttactac gtctctttta gcagtggtga tttagaagct agcggtaaac gctatccgat
    24421 tgatgtgaaa atgattgcac catttagtga tgaggtcatg aaagtcactg gccttaatgg
    24481 caaagcaagt tctgcaaaag tacattttta cgccattaat gattttggtg gcgccattga
    24541 aggtaatgcc agtctgtaat caggcaagga tataattccg caggaagcat aacgtgacta
    24601 taaaaccaac taaaaattat cataatcatc tgacccgtat cgctacgttt tgcgcgctgc
    24661 tgtattgcaa tagtgctttc tgtgctgaac ttgttgaata tgaccatacc ttcctgatgg
    24721 ggcagaatgc ctcaaatatt gatctcagcc ggtatagtga aggtaaccct gctataccgg
    24781 gtatgtacga cgtcagtgtt tatgtcaacg atcaacctat tattaaccaa agcattacat
    24841 ttattgaaat tgaaggaaaa aaaacgcaca agcctgtatc acacttaaga atttattgca
    24901 gtttcatata aattctcccg atataaataa cgaaaaagcc gttctgcttg ccagggatga
    24961 aacactcggc aattgcctta atttgacgga aattatccct caggcttctg ttcgttatga
    25021 cgttaacgag caacgtctgg atatagacgt tcctcaggca tgggtaatga aaaattacca
    25081 aaactatgtt gatccatcgt tatgggaaaa tggcattaat gcggccatgt tgtcatacaa
    25141 cctcaacgga tatcatagtg aaactcctgg tcgaagaaat gacagcattt atgctgcttt
    25201 taacggtggg atgaatttag gtgcatggcg actgcgtgcc tcaggcaact acaactggat
    25261 gaccgattct ggcagtaatt atgattttaa gaatcggtat atacagcgtg atatcgcctc
    25321 gctgcgttct caactcattc ttggtgagtc ttatacaacg ggcgaaacct ttgattctgt
    25381 cagtatccgc ggcattcgtt tatacagcga cagccgcatg ttgcctccga cattagcgag
    25441 ttttgcgcct atcattcatg gcgttgctaa taccaacgcc aaagtcacta ttacgcaagg
    25501 tggctataag atttatgaaa cgactgtgcc gccaggcgct ttcgtcattg atgatctgag
    25561 tccgtcaggt tacggcagcg atcttattgt tactgtcgaa gaatccgatg gctcaaagcg
    25621 gacattctcg caaccttttt catcggtcgt tcaaatgtta cgccctggcg ttggacgttg
    25681 ggatattagc ggcggccagg tcttaaaaga tgatattcag gatgagccta atttatttca
    25741 ggccagctac tactacggtc tgaataacta tctaacg
//
LOCUS       DAJVYM010000044        25735 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVYM010000044 DAJVYM010000000
VERSION     DAJVYM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.044"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(301..507)
                     /locus_tag="OQS60_001718"
     CDS             complement(301..507)
                     /locus_tag="OQS60_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS2387497.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="OQS60_001719"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="OQS60_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS2387498.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="OQS60_001720"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="OQS60_001720"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS2387499.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="OQS60_001721"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="OQS60_001721"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS2387500.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="OQS60_001722"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="OQS60_001722"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS2387501.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="OQS60_001723"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="OQS60_001723"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS2387502.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="OQS60_001724"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="OQS60_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS2387503.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8860..8964
                     /locus_tag="OQS60_001725"
     CDS             8860..8964
                     /locus_tag="OQS60_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS2387504.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9275..11473
                     /gene="speF"
                     /locus_tag="OQS60_001726"
     CDS             9275..11473
                     /gene="speF"
                     /locus_tag="OQS60_001726"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS2387505.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11470..12789
                     /gene="potE"
                     /locus_tag="OQS60_001727"
     CDS             11470..12789
                     /gene="potE"
                     /locus_tag="OQS60_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS2387506.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OQS60_001728"
     CDS             complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OQS60_001728"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS2387507.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OQS60_001729"
     CDS             complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OQS60_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS2387508.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15241..16005
                     /gene="ybfF"
                     /locus_tag="OQS60_001730"
     CDS             15241..16005
                     /gene="ybfF"
                     /locus_tag="OQS60_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS2387509.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16145..16438
                     /gene="ybfE"
                     /locus_tag="OQS60_001731"
     CDS             16145..16438
                     /gene="ybfE"
                     /locus_tag="OQS60_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS2387510.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16578..17108
                     /gene="fldA"
                     /locus_tag="OQS60_001732"
     CDS             16578..17108
                     /gene="fldA"
                     /locus_tag="OQS60_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS2387511.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17318..17404
                     /gene="uof"
                     /locus_tag="OQS60_001733"
     CDS             17318..17404
                     /gene="uof"
                     /locus_tag="OQS60_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS2387512.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17397..17843
                     /gene="fur"
                     /locus_tag="OQS60_001734"
     CDS             17397..17843
                     /gene="fur"
                     /locus_tag="OQS60_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS2387513.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17928..18254)
                     /locus_tag="OQS60_001735"
     CDS             complement(17928..18254)
                     /locus_tag="OQS60_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387514.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OQS60_001736"
                     /pseudo
     CDS             complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OQS60_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OQS60_001737"
     CDS             complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OQS60_001737"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS2387515.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OQS60_001738"
     CDS             complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OQS60_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS2387516.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24490..25290
                     /gene="nagB"
                     /locus_tag="OQS60_001739"
     CDS             24490..25290
                     /gene="nagB"
                     /locus_tag="OQS60_001739"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS2387517.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25350..>25735
                     /locus_tag="OQS60_001740"
     CDS             25350..>25735
                     /locus_tag="OQS60_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS2387518.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGL"
BASE COUNT         6025 a         6543 c         6734 g         6433 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
     8701 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
     8761 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
     8821 aactctacct tcaactgaca cagcaagagg taaaggtaaa tggaaaataa taaccgctta
     8881 atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa tagttttgac
     8941 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
     9001 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
     9061 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
     9121 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
     9181 tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga gaaattgagg
     9241 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
     9301 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
     9361 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
     9421 tgaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
     9481 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
     9541 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
     9601 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     9661 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     9721 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     9781 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     9841 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     9901 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     9961 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
    10021 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
    10081 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
    10141 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
    10201 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
    10261 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctggt
    10321 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
    10381 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
    10441 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
    10501 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
    10561 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
    10621 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
    10681 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
    10741 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
    10801 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
    10861 agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat
    10921 ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    10981 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11041 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11101 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11161 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgccga
    11221 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11281 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11341 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11401 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11461 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11521 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11581 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11641 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11701 tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt ctcactgctg
    11761 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11821 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    11881 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc
    11941 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12001 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12061 atgacgctgt gggcttttct cggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12121 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12181 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12241 acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg taaagtcatt
    12301 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12361 caggtgttta agtcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12421 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12481 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12541 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12601 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12661 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12721 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12781 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt tttattaaat
    12841 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    12901 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    12961 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    13021 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    13081 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13141 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13201 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    13261 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13321 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    13381 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13441 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13501 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13561 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcaat gccagcaggc
    13621 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcgaggtgca
    13681 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13741 cgccaattcg cttccagtat tcgataccgg aaccacccag cggatcaacg cccagcgtca
    13801 ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaactgct
    13861 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    13921 tcacgccttt caggccatcg gccatcagtg cgttggcacg atcttccacc actttagtga
    13981 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14041 ggttatggga gggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattatgaa
    14101 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14161 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14221 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14281 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14341 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14401 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14461 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14521 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14581 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14641 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    14701 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14761 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14821 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    14881 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    14941 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15001 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15061 atccagtaca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15121 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15181 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15241 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15301 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15361 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    15421 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    15481 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    15541 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15601 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    15661 actcgccagc aagcagcagc aataatgcac cagcatctta atgaagaagg ggtgattcag
    15721 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15781 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15841 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    15901 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    15961 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16021 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16081 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16141 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16201 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16261 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16321 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    16381 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16441 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16501 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16561 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16621 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16681 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    16741 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16801 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16861 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    16921 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    16981 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17041 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    17101 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17161 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    17221 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17281 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17341 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17401 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17461 tcctagaagt tcttcaggaa ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17521 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17581 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17641 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    17701 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17761 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17821 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    17881 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    17941 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    18001 tgcgttgcac gcagatactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    18061 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    18121 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    18181 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    18241 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    18301 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18361 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18421 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18481 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18541 ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18601 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    18661 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18721 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    18781 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18841 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    18901 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    18961 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19021 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    19081 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19141 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19201 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    19261 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19321 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19381 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19441 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19501 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19561 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19621 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    19681 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19741 gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    19801 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    19861 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    19921 gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc cagcaagatg aaagcggtta
    19981 cattttattt aattatgttt tgttatttta aatttatttt atggataaat tgcgaagcgg
    20041 aattaattcg cgaagaaaag cagaaaaaac gccgctgaac gcggcgtttt ggattgtttg
    20101 catatttctt gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc
    20161 aatatattgc agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt
    20221 gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa
    20281 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20341 ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact
    20401 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20461 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20521 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20581 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20641 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
    20701 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20761 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc
    20821 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
    20881 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
    20941 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21001 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    21061 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21121 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21181 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21241 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21301 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21361 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21421 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21481 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21541 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21601 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21661 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21721 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21781 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21841 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21901 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21961 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22021 tgttagttag cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    22081 agcggcagag aagatctcca ccgcttttgc cagtcccgcg tcattgttgg atgcgcgtct
    22141 tatccaacca ggactgagct tttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
    22201 ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acattgccct
    22261 gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca
    22321 tcgaacgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc
    22381 tcacctgcgc gccctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    22441 cgatacccat gtggacgaca atctccgcgc ctttttcggt ttctaggcag aacgcgtggt
    22501 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    22561 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca gaaacctgat
    22621 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    22681 gtacagcctg cggttttgct acaggagcgg cagttgccgg agttgcttca gctgacgcag
    22741 ctgctaccgg gccacgggca acgactttct tcatcgcatc gccaatggat tctgctttcg
    22801 cgccaacaat cacctgaatg gtctgtttgt tcagttttac tacaccagaa gcacccagac
    22861 gtttacacat cgcatcgttg acgcgagctg agtcagccac ggtaaggcgc agacgggtaa
    22921 tacaggcgtc aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg
    22981 ccagttgagt cagaccttct tcagtattgc tgttagcctc ttcagttacg atctcgtctt
    23041 ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca
    23101 cgaagtagat agcgaagaag acaacaccca tcaccagcag catccagacg ttctggctgg
    23161 cggccggcag gttatacatc aacgcgtagt cgatagcccc cgcagagaaa gagaagccag
    23221 catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    23281 ggtacagcag cggagcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    23341 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    23401 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga
    23461 agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt
    23521 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    23581 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    23641 ccagcgcgcc tgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtactggcg
    23701 gccagacgta accaaaaatg gccgccagca ccaggcagaa aaagccggtg gcaatcggca
    23761 caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt
    23821 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    23881 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    23941 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    24001 cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    24061 gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg agctgtaacg
    24121 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    24181 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt
    24241 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt
    24301 aaacgcgtgc cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    24361 gctaaagcag aaaacttatt ttatcattca aaaaatcagg tcggattgac gcctgtctgc
    24421 gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt ttacttattg
    24481 aggtgaataa tgagactgat ccccctaact accgctgaac aggtcggcaa atgggctgct
    24541 cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc gtttgttctg
    24601 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    24661 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    24721 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    24781 gatattccag cagaaaacat caaccttctc aacggcaacg ccccggatat cgacgccgaa
    24841 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    24901 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    24961 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    25021 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct ggatgccgaa
    25081 gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc tgcagttgaa
    25141 ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg
    25201 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    25261 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct ccttgctcag
    25321 ggcaatattt tttaaaatcg gggggcagaa tgtatgcatt aacccagggc cggatcttta
    25381 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    25441 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    25501 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    25561 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    25621 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    25681 tgcgcgttat gcgcgagtac ctggcaagac atccgaatca ggcgttaggt ctgca
//
LOCUS       DAJVYM010000045        25495 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVYM010000045 DAJVYM010000000
VERSION     DAJVYM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.045"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            16..486
                     /locus_tag="OQS60_001741"
     CDS             16..486
                     /locus_tag="OQS60_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCS2387519.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            488..907
                     /gene="gspB"
                     /locus_tag="OQS60_001742"
     CDS             488..907
                     /gene="gspB"
                     /locus_tag="OQS60_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCS2387520.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1145..1456
                     /gene="rpsJ"
                     /locus_tag="OQS60_001743"
     CDS             1145..1456
                     /gene="rpsJ"
                     /locus_tag="OQS60_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS2387521.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1489..2118
                     /gene="rplC"
                     /locus_tag="OQS60_001744"
     CDS             1489..2118
                     /gene="rplC"
                     /locus_tag="OQS60_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS2387522.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2129..2734
                     /gene="rplD"
                     /locus_tag="OQS60_001745"
     CDS             2129..2734
                     /gene="rplD"
                     /locus_tag="OQS60_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS2387523.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2731..3033
                     /gene="rplW"
                     /locus_tag="OQS60_001746"
     CDS             2731..3033
                     /gene="rplW"
                     /locus_tag="OQS60_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS2387524.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3051..3872
                     /gene="rplB"
                     /locus_tag="OQS60_001747"
     CDS             3051..3872
                     /gene="rplB"
                     /locus_tag="OQS60_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS2387525.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3889..4167
                     /gene="rpsS"
                     /locus_tag="OQS60_001748"
     CDS             3889..4167
                     /gene="rpsS"
                     /locus_tag="OQS60_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS2387526.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4182..4514
                     /gene="rplV"
                     /locus_tag="OQS60_001749"
     CDS             4182..4514
                     /gene="rplV"
                     /locus_tag="OQS60_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS2387527.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4532..5233
                     /gene="rpsC"
                     /locus_tag="OQS60_001750"
     CDS             4532..5233
                     /gene="rpsC"
                     /locus_tag="OQS60_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS2387528.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5246..5656
                     /gene="rplP"
                     /locus_tag="OQS60_001751"
     CDS             5246..5656
                     /gene="rplP"
                     /locus_tag="OQS60_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS2387529.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5656..5847
                     /gene="rpmC"
                     /locus_tag="OQS60_001752"
     CDS             5656..5847
                     /gene="rpmC"
                     /locus_tag="OQS60_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS2387530.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5847..6101
                     /gene="rpsQ"
                     /locus_tag="OQS60_001753"
     CDS             5847..6101
                     /gene="rpsQ"
                     /locus_tag="OQS60_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS2387531.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6266..6637
                     /gene="rplN"
                     /locus_tag="OQS60_001754"
     CDS             6266..6637
                     /gene="rplN"
                     /locus_tag="OQS60_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS2387532.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6648..6962
                     /gene="rplX"
                     /locus_tag="OQS60_001755"
     CDS             6648..6962
                     /gene="rplX"
                     /locus_tag="OQS60_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS2387533.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6977..7516
                     /gene="rplE"
                     /locus_tag="OQS60_001756"
     CDS             6977..7516
                     /gene="rplE"
                     /locus_tag="OQS60_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS2387534.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7531..7836
                     /gene="rpsN"
                     /locus_tag="OQS60_001757"
     CDS             7531..7836
                     /gene="rpsN"
                     /locus_tag="OQS60_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS2387535.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7870..8262
                     /gene="rpsH"
                     /locus_tag="OQS60_001758"
     CDS             7870..8262
                     /gene="rpsH"
                     /locus_tag="OQS60_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS2387536.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8275..8808
                     /gene="rplF"
                     /locus_tag="OQS60_001759"
     CDS             8275..8808
                     /gene="rplF"
                     /locus_tag="OQS60_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS2387537.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8818..9171
                     /gene="rplR"
                     /locus_tag="OQS60_001760"
     CDS             8818..9171
                     /gene="rplR"
                     /locus_tag="OQS60_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS2387538.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9186..9689
                     /gene="rpsE"
                     /locus_tag="OQS60_001761"
     CDS             9186..9689
                     /gene="rpsE"
                     /locus_tag="OQS60_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS2387539.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9693..9872
                     /gene="rpmD"
                     /locus_tag="OQS60_001762"
     CDS             9693..9872
                     /gene="rpmD"
                     /locus_tag="OQS60_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS2387540.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9876..10310
                     /gene="rplO"
                     /locus_tag="OQS60_001763"
     CDS             9876..10310
                     /gene="rplO"
                     /locus_tag="OQS60_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS2387541.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10318..11649
                     /gene="secY"
                     /locus_tag="OQS60_001764"
     CDS             10318..11649
                     /gene="secY"
                     /locus_tag="OQS60_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS2387542.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11681..11797
                     /gene="rpmJ"
                     /locus_tag="OQS60_001765"
     CDS             11681..11797
                     /gene="rpmJ"
                     /locus_tag="OQS60_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS2387543.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11944..12300
                     /gene="rpsM"
                     /locus_tag="OQS60_001766"
     CDS             11944..12300
                     /gene="rpsM"
                     /locus_tag="OQS60_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS2387544.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12317..12706
                     /gene="rpsK"
                     /locus_tag="OQS60_001767"
     CDS             12317..12706
                     /gene="rpsK"
                     /locus_tag="OQS60_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS2387545.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12740..13360
                     /gene="rpsD"
                     /locus_tag="OQS60_001768"
     CDS             12740..13360
                     /gene="rpsD"
                     /locus_tag="OQS60_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS2387546.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13386..14375
                     /gene="rpoA"
                     /locus_tag="OQS60_001769"
     CDS             13386..14375
                     /gene="rpoA"
                     /locus_tag="OQS60_001769"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS2387547.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14416..14799
                     /gene="rplQ"
                     /locus_tag="OQS60_001770"
     CDS             14416..14799
                     /gene="rplQ"
                     /locus_tag="OQS60_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS2387548.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14906..15274
                     /locus_tag="OQS60_001771"
     CDS             14906..15274
                     /locus_tag="OQS60_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS2387549.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15285..15710
                     /gene="zntR"
                     /locus_tag="OQS60_001772"
     CDS             15285..15710
                     /gene="zntR"
                     /locus_tag="OQS60_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS2387550.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15766..15984
                     /gene="arfA"
                     /locus_tag="OQS60_001773"
     CDS             15766..15984
                     /gene="arfA"
                     /locus_tag="OQS60_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS2387551.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="OQS60_001774"
     CDS             complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="OQS60_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS2387552.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="OQS60_001775"
     CDS             complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="OQS60_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS2387553.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="OQS60_001776"
     CDS             complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="OQS60_001776"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS2387554.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="OQS60_001777"
     CDS             complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="OQS60_001777"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS2387555.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20216..20725)
                     /gene="def"
                     /locus_tag="OQS60_001778"
     CDS             complement(20216..20725)
                     /gene="def"
                     /locus_tag="OQS60_001778"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS2387556.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20855..21979
                     /gene="dprA"
                     /locus_tag="OQS60_001779"
     CDS             20855..21979
                     /gene="dprA"
                     /locus_tag="OQS60_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS2387557.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21951..22424
                     /locus_tag="OQS60_001780"
     CDS             21951..22424
                     /locus_tag="OQS60_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS2387558.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22453..22995
                     /locus_tag="OQS60_001781"
     CDS             22453..22995
                     /locus_tag="OQS60_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS2387559.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23000..23572
                     /gene="tsaC"
                     /locus_tag="OQS60_001782"
     CDS             23000..23572
                     /gene="tsaC"
                     /locus_tag="OQS60_001782"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS2387560.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23577..24395
                     /gene="aroE"
                     /locus_tag="OQS60_001783"
     CDS             23577..24395
                     /gene="aroE"
                     /locus_tag="OQS60_001783"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS2387561.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24392..24649
                     /locus_tag="OQS60_001784"
     CDS             24392..24649
                     /locus_tag="OQS60_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS2387562.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24625..25179)
                     /locus_tag="OQS60_001785"
     CDS             complement(24625..25179)
                     /locus_tag="OQS60_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS2387563.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6022 c         6680 g         6301 t
ORIGIN      
        1 gcttctttac aaacattggt agatcagaat ttgccctgga gcgcatcgct taaagtcggg
       61 gacaaaaaat tgcctgttgt ggtcgttcgc gtcggtgagg ctagcgttga tgtcctcgta
      121 ggtcagcaaa cgtggacgct gacacataaa tggtttgagt cggtatggac gggtgattat
      181 cttctgttgt ggaaaatgtc gccggaaggg gagagtacca ttacgcgtga tagcagcgag
      241 gaagagattc tctggctgga aacgatgtta aaccgtgcat tgcatatttc gacagaacct
      301 tcggcggagt ggcgtccctt gttggtagaa aaaataaagc aatttcaaaa aagccaccac
      361 ttgaaaactg acggtgttgt tggcttttca acgttagtac atctctggca ggtggcaggg
      421 gagagtgcct acttatatcg ggatgaagca aatatttccc cggaaacaac agtgaagggg
      481 aaatgacatg tttgaattct atatcgccgc gcgtgagcag aaagaaacag gtcaccctgg
      541 tatcttctcc cgacaaaaac acagcaccat aatatatgtc atttgtttat tgctgatttg
      601 cctgtggttt gcaggaatgg tgttggttgg tgggtatgcc aggcagctat gggcgctttg
      661 gatagtaaaa gcagaagtca ctgtagaggc tgaaacgccc gcttttaaac aatcaacaca
      721 acactatttc ttcaaaaaac agccactccc cgtcgtcgaa tccgtcgagg aggaagatga
      781 cccagacgtc gcggttgaga atgctccctc ttcttctgaa gacgaagaaa atactgtaaa
      841 agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa aatgcgctga acgaacttga
      901 gagataaccc gaaggctgtt tactttacta gcaatacgct tgcgttcggt ggttaagtat
      961 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1021 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1081 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1141 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1201 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1261 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     1321 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     1381 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     1441 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     1501 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     1561 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     1621 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     1681 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     1741 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgaggtt
     1801 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     1861 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     1921 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     1981 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2041 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2101 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2161 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2221 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     2281 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     2341 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     2401 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     2461 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     2521 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     2581 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     2641 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     2701 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     2761 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     2821 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     2881 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     2941 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3001 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3061 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3121 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3181 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3241 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     3301 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     3361 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     3421 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     3481 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     3541 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     3601 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     3661 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtaccg
     3721 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     3781 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     3841 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     3901 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     3961 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4021 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtgaccgac
     4081 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4141 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact atcgctaaac
     4201 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4261 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtactgg
     4321 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     4381 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     4441 cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc actgtggttg
     4501 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     4561 tcgcttgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     4621 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     4681 gtctgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     4741 tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac tgcgtaaggt
     4801 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     4861 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     4921 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     4981 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5041 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5101 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5161 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5221 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     5281 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     5341 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     5401 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     5461 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     5521 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     5581 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     5641 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcgttga agagctgaac
     5701 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     5761 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     5821 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     5881 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     5941 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6001 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6061 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6121 acgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6181 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6241 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     6301 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     6361 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     6421 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     6481 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     6541 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     6601 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     6661 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     6721 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     6781 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     6841 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     6901 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     6961 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7021 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7081 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7141 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7201 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7261 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtattc gtgacttccg
     7321 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     7381 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     7441 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     7501 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     7561 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     7621 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     7681 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgcccg
     7741 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     7801 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     7861 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     7921 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     7981 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8041 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8101 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8161 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8221 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     8281 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     8341 gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa
     8401 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     8461 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     8521 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     8581 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     8641 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ttgataagca ggtgatcggc
     8701 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttataaagg caagggtgtt
     8761 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     8821 gataagaaat ttgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     8881 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     8941 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9001 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9061 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9121 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9181 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9241 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
     9301 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
     9361 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
     9421 gcactctgca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
     9481 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
     9541 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
     9601 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
     9661 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
     9721 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ctgggtctgc
     9781 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
     9841 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
     9901 ccgaaggctc caaaaaggcg ggtaaacgct tgggtcgtgg tatcggttct ggcctcggta
     9961 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10021 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10081 gtaaagcagc gattacagcc gaagttcgtc tgtctgacct ggctaaagta gaaggcggtg
    10141 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10201 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10261 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    10321 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    10381 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    10441 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    10501 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    10561 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    10621 ttggcggaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgc
    10681 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctactgg tctgccgaat
    10741 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    10801 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat tactgaacga
    10861 ggtatcggca acggtatttc aatcattatc ttcgtcggta ttgtcgcggg actcccgcca
    10921 gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg
    10981 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tatttgttga gcgtggtcaa
    11041 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgttta tgctgcacag
    11101 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11161 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11221 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    11281 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    11341 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    11401 acggcgaagt atatcgataa agtaatgacc cgcctgaccc tggttggtgc gctgtatatt
    11461 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    11521 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    11581 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    11641 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    11701 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    11761 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    11821 aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat
    11881 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    11941 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12001 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12061 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12121 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12181 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12241 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    12301 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    12361 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    12421 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    12481 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    12541 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    12601 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    12661 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    12721 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    12781 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    12841 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    12901 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    12961 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13021 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13081 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13141 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13201 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga
    13261 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    13321 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    13381 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    13441 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    13501 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    13561 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    13621 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    13681 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    13741 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    13801 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    13861 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    13921 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    13981 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14041 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14101 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14161 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14221 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    14281 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcctgg
    14341 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    14401 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    14461 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    14521 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    14581 aagactgata gcgttgctaa tcgtcgtctg gcatttgccc gtactcgtga taacgagatc
    14641 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    14701 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    14761 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac
    14821 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    14881 tttttgttca tcccctggag tatttatgtg gttacttgac cagtgggcag agcgccatat
    14941 agcagaagcg caagcgaaag gtgagtttga taacctggca ggtagcggcg aaccattgat
    15001 actggatgat gattctcacg tgccaccgga attacgtgcg gggtatcgct tgctgaagaa
    15061 tgccggttgc ttaccgccag aacttgagca acggagagaa gcaattcagc ttctggatat
    15121 tctcaaaggt atccgtcacg atgatccgca atatcaagag gttagccgtc gattgtcatt
    15181 actggaattg aagctgcgac aagctggatt gagtaccgat tttttacgcg gcgattatgc
    15241 tgacaagttg ttggacaaaa tcaacgataa ctagtggagt atgtatgtat cgcattggtg
    15301 agctggcaaa aatggcggaa gtaacacctg acacgattcg ttattacgaa aaacagcaga
    15361 tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct gtataccgaa agcgatctcc
    15421 agcgattgaa atttatccgc catgccagac aactaggttt cagtctggag tcgatccgcg
    15481 agttgctgtc gatccgcatc gatcctgaac accatacctg tcaggagtca aaaggcattg
    15541 tgcaggaaag attgcaggaa gtcgaagcac ggatagccga gttgcagagt atgcagcgtt
    15601 ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt tattgctcga
    15661 ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag tggctgttga ttttttgcac
    15721 tgacaggatt acactcgcgc cgttaaataa ccaactggag tttttatgag ccgatatcag
    15781 catactaaag ggcagataaa ggataatgcg atagaagcat tactacatga tcccttattc
    15841 cgacagcgag tagagaaaaa taagaaaggg aaaggcagtt acatgcgaaa aggaaaacat
    15901 ggcaatcggg ggaactggga ggccagtggc aagaaagtaa atcacttttt taccactggt
    15961 cttctgcttt caggcgcttg ttaagagcgg ttattctgct ctttcagcaa atcacgaatt
    16021 tctgccagta atacttcttc tttagttggt gcaggtgcgg cagctggttc ttcttttttc
    16081 cgattcagtt tgttgattag cttaatcgcc ataaagatgg caaaggccac aatcagaaaa
    16141 tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat atccccctgc
    16201 gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc cgccaattaa taagcccaga
    16261 ggtggcatga tgatatcggc aaccagtgaa gatacaatct tcccgaatgc cgcaccgata
    16321 atgacaccca ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg aaattcttta
    16381 ataatgctca tgttattctc cctatgaagc cgacaaccat aagtctaaca aatgttaagc
    16441 cattttcctg ccaggaatga atttaattaa tccttgataa tcaaagggaa ataataaggc
    16501 gtcattagac gccttattaa ttacaagaag aaagggcttg gctggaagag tctttcgacg
    16561 tcggtaataa actttttatc tgtgaggaac ataattacgt gatcgccttg ctcaatgcgc
    16621 agattgtcat tggcaatcat cacgtcgttt ccacgtacca ccgctccaat aatcgttcct
    16681 ggcggtagct tgatttcgtc aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg
    16741 tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca atgaggaaac accaacaata
    16801 tctgcttttc gcacatggct aagcaacgca gaaatagttg cttgttgtgg tgaaattgca
    16861 atatcgatca cgctcccctg aaccagatcc acataagcgc gacgctggat caataccatc
    16921 acctttttcg cacccatacg tttggcaagc atggcggaca tgatattggc ctcgtcatcg
    16981 ttggtgacgg caataaacag atcaacttga tcgatatgtt cttcggccag cagttcttga
    17041 tccgacgcat caccaaaaaa gacgatcgta ttctgcaact tttccgccag ttcggcagcg
    17101 cgctgctgat tacgttcgat gagtttaacg ctgtaatctt tttccagacg acgcgccagc
    17161 cctgcaccga tattaccgcc accaaccagc atgatccgct tatacggttt ttccagtcgc
    17221 tgtaattcac tcatcaccgc gcggatatgc tgtgaagcgg caataaagaa cacttcatca
    17281 ccagcttcaa caatggtcga accttgtgga cgaatggggc gatcgtggcg gaaaattgct
    17341 gccacacgag tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc
    17401 agcgggccgc cataataggc tttaactaca gcgaggctga ctttaccctc agcgaagttc
    17461 accacctgca atgcgcctgg atactcaatc agtcggtaaa tattatcgat aaccaactgc
    17521 tctggtgcga tcagatgatc aatcggtaca gcatctgaat gaaatagctt atcggcatcg
    17581 cgtacgtagt ctggtgagcg gatgcgagcg atgcgattag gggtgttgaa aagtgagtag
    17641 gctacctggc aggcaaccat attggtttca tctgaactgg ttacagcaac cagcatatcg
    17701 gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag agccatgccc ctgcacgact
    17761 cgcaggtcaa atttatcctg caaggtccgc agacgctcac cgttggtatc gacaacagta
    17821 atatcgttgt tctcgccaac caggttttcc gccagtgtgc cgccaacctg gccggcaccc
    17881 agaatgataa ttttcatcag tctcgacccg ttatctcatc actttttgat tagcttagcg
    17941 taaaagaagc cgtcgccctc ttcggcacca ggcagatttt gtttacccgg ttgctctggt
    18001 gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca aaaaagcttt aatctgcagg
    18061 ctattctctt ccggtaacac cgaacaggtg gcatagacca gggttccacc ggattttaaa
    18121 tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg cgagttccgg gatatcgcga
    18181 tcgcgacgta accatttaat atccggatgg cgacgaatca cccccgttgc tgaacaaggc
    18241 gcatctaata aaatgcgatc aaactgttgc tcgccacacc attgggaagg gtaacggcca
    18301 tcaccttgtt tcacggtcgc cttcatacca aggcgtttta aattgtcgtg aacgcgagag
    18361 aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac ctcaaggata
    18421 tgcgttgttt taccgccggg ggcggcacaa agatccagaa tgtgttcacc gttttgtggc
    18481 gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa cggtaaccca tccgtcttca
    18541 aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc gtacagcatc agggtaatcc
    18601 gcatgcggga aacctttcat tcctgcttca tccaacaatg caagccagct gtcgcgggaa
    18661 tgatgcgtac gattaacgcg cagccacatt ggcggacgct ggttattggc ttcgacgatg
    18721 gattgccact gctctggata cgctttttgc agacgcttca gcaaccagga aggatgcaga
    18781 taacgtgcat cactggtatt aaactcggct aataactctt cttgctgacg ctggaactgg
    18841 cgtaatacac cgttaatcaa ccctttaagt tgcggacgct taattgcgat agcgccttca
    18901 accgtttcag ccagcgcagc atgaggtgga atgcgggtat aaagcagttg atacaaacca
    18961 accataatca ggtaatgcac agtgcgctgt ttgccggtca tcggacgggc cattaactta
    19021 ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga
    19081 agtgctttgt ctttatcaga aactttttgc tggagcggtg gcagaatgtt gcttaatgat
    19141 tgcccttgct cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa attacgttgt
    19201 tttttcataa gtataaaaat gcccggtaag accgggctta gaagagtgga ctatcaggcc
    19261 agacggttgc ccggaacaaa ccattcccga cgagaattca ggaggtcttg cgcgctcatc
    19321 gctttcttac ccgcaggttg taacgagagc aggttcagga tgccatcacc agtcgcaacc
    19381 tgaatgcctt gtttgttggc ttcaaggatc gttcctggtg cagcgttggt tgccgtatca
    19441 atgaccgacg ctttccagac tttaaccggt tgtccttcaa tttccagcca gctcattggc
    19501 catgggttga aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga ccagtcaata
    19561 cgcgcttctt ctttactcaa cttctcggcg taagtgacaa gagtttcgtc ctgaacttct
    19621 ggtttcgccg tgccgtctgc tagttgtttc aacgtggtga ttagcccttg tgggccaagc
    19681 tctgccagct tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg gcaggagagc
    19741 ttatagagca tatcaccggt gtctaaaccg acatccattt gcataatggt cacaccagtt
    19801 tctgcatcac ccgcccatag tgagcgttgg attggtgcag caccgcgcca gcgtggcagc
    19861 agtgaaccat gaacgttgat acagccaaga cgcggcatct ccagcactgc tttaggcaga
    19921 attaagccat aggcgacgac gaccataaca tcagcctgca gatcggcgac cagttgctgg
    19981 ctttcttgtg gacgcaggga aacaggttga aaaacgggca gacctttttc ctcagccaga
    20041 actttaaccg ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg
    20101 aacacgccaa cgacgttatg accagaagac aacagcgcgt cgagatgacg cgctgcaaag
    20161 tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca cgttagttct tatccttaag
    20221 cccgggcttt cagacgatct agtttttcaa ctttctgacg aatacgttgt tgtttcagcg
    20281 gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga
    20341 tggctaacag cccctctgct tccagttcaa atggtttacc gtcgcggtca agggcgcgaa
    20401 ttttaacttt ctctgcgcgc ggcactaaag cacgttgttc agggatcgac aggcaacctt
    20461 cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt
    20521 cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg
    20581 ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct
    20641 gtatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa
    20701 tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcagggg ttagaaagat
    20761 tatttcttct attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa
    20821 gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg
    20881 tttaatgagt atcagcagct tgtacggcga tgatatggtc cgtatagctc actggctggc
    20941 aaaacagtcg caaattgatg cggttggatt gcagcaaaca gggcttacat tgcggcaggc
    21001 acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt ggttggagca
    21061 acccaaccat catttaatcc ctgcggacag cgaattttat cctcctcaac ttctggtgac
    21121 gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca
    21181 gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg gacggttatt
    21241 ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga
    21301 tggtgtggcg cataaagctg ccttacaggt aaatggcgtc agcattgctg tattggggaa
    21361 tggacttaat accattcatc cccgccgcca tgcccgactg gctgccagtc tgcttgaaca
    21421 tggcggagct ctcgtctcgg aatttcccct cgatgttcca ccccttgctt acaatttccc
    21481 acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt
    21541 gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag cagaggcgtg aagtttttgc
    21601 cttgccaggt ccaataggga atccgggaag cgaagggcct cactggttaa taaaacaagg
    21661 tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg
    21721 gttgccagac gcccctgaaa attcatttta ttcaccagat cagcaagacg tggcattgcc
    21781 atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga
    21841 acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg agttagcagg
    21901 atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg
    21961 tactaatgta tttgtttgaa acctatattc acactgaagc tgagttgcgt gtggatcaag
    22021 acaaacttga acaggatctt accgacgcag gttttgagcg agaagatatc tacaatgccc
    22081 tgctatggct ggaaaaactt gctgattatc aggaagggtt ggcagaaccg atgcaactgg
    22141 cctcggatcc tctctccatg cgtatttata caccggaaga gtgtgaacga ctggatgcca
    22201 gctgccgtgg gtttctgctt ttccttgagc agattcaggt gctcaacctt gaaactcgtg
    22261 aaatggtgat agagcgagtg ctcgcgctgg ataacgcaga gttcgaactg gatgatctga
    22321 aatgggtgat cctgatggtg ttgttcaaca tcccggggtg cgaaaatgcg taccagcaaa
    22381 tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca
    22441 gcataagttg ttatggcaaa atcagcactg ttcacggtgc gtaataatga gtcctgccca
    22501 aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggatgctca
    22561 cagtatccgg cgtgtgacta cgtccgtctt ctgaaatctt cagcggatgg acatattgtc
    22621 aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga
    22681 cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc
    22741 gataaaccgg acgaaacagc aattacatgc ccccaatgtc ggacgggcca tctggtccag
    22801 cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga gtgtcaattt
    22861 gccattaact tcaaaccaat agctggagaa tgccctgagt gtcattatcc gctactcatc
    22921 gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg
    22981 gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat
    23041 agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg
    23101 gtgcgatcct gatagcgaaa cagcagtgat gagactgttg gagttaaaac agcgtccggt
    23161 tgataagggg ctgattttaa tcgcggcaaa ttacgagcag cttaaaccct atattgatga
    23221 caccatgctg actgatgcgc agcgtgaaac cattttttcc cgctggccag gtcctgtcac
    23281 ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct
    23341 tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaggctt atggtaaacc
    23401 gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt
    23461 tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa
    23521 tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg
    23581 aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc
    23641 aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg
    23701 atttcatcaa cacactgaac gctttctttc gtgctggtgg taaaggtgcg aatgtgacgg
    23761 tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg
    23821 ctggtgcagt taataccctc aagcggttag aagatggacg cctgctgggt gacaataccg
    23881 atggtgtagg cttgttaagc gatctggaac gtctgtcttt tatccgccct ggtttacgta
    23941 ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactt ctttccctag
    24001 actgtgcggt gacaatcact aatcggacgg tatcccgcgc ggaagagttg gctaaattgt
    24061 ttgcgcacac tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg
    24121 atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat
    24181 cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt
    24241 ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg
    24301 tggcacaggc ggctcatgcc ttccttcttt ggcatggcgt tctgcctgat gtagaaccgg
    24361 tcatcaaact attgcaacag gaattgtcag cgtgaatcag gccatccagt ttccggatag
    24421 ggaagagtgg gacgagaata aaagatgtgt atgttttcct gctctcgtga atggtatgca
    24481 actgacatgt gcgatctctg gcgagagtct ggcgtatcgc tttactggag gtacgccaga
    24541 acagtggtta gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt
    24601 aattcaggaa caaagtgaag atgatcaagg ttgggtctgg ttaccttgat ccagatattc
    24661 gtccttccat ttcacgtagt tatttgcgga atagcgtaac ccagccttct cttcatcact
    24721 taacgggcgg atctgtttga cgggactacc gagatacaga tatccgctct ccagccgttt
    24781 attttgtggg accaggctac ccgcaccaat catcacatca tcttctacta ttgcgccatc
    24841 aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat
    24901 caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta
    24961 cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat
    25021 ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc
    25081 cagacgaacg tcaccaatca cgacgctgct atcgtcgatc attacgcgct ggccgatttg
    25141 tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata ctcacctcaa caataaatga
    25201 tttcctaatg actttgggtg cattattagc cttgtgcaag tcttttagta tgcaaaaaag
    25261 caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat
    25321 caaaaaagta cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa
    25381 cgtgaaacac ttcacaggat ggtcggaaca acgaagagaa aaaattctga aattcagggt
    25441 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaag
//
LOCUS       DAJVYM010000046        25429 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVYM010000046 DAJVYM010000000
VERSION     DAJVYM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.046"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OQS60_001786"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OQS60_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS2387564.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OQS60_001787"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OQS60_001787"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS2387565.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="OQS60_001788"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="OQS60_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OQS60_001789"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OQS60_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS2387566.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="OQS60_001790"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="OQS60_001790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS2387567.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="OQS60_001791"
     CDS             complement(6490..6846)
                     /locus_tag="OQS60_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS2387568.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="OQS60_001792"
     CDS             complement(6850..7266)
                     /locus_tag="OQS60_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS2387569.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OQS60_001793"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OQS60_001793"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS2387570.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="OQS60_001794"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="OQS60_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OQS60_001795"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OQS60_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS2387571.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OQS60_001796"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OQS60_001796"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS2387572.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OQS60_001797"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OQS60_001797"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS2387573.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="OQS60_001798"
     CDS             13294..14277
                     /locus_tag="OQS60_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS2387574.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OQS60_001799"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OQS60_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS2387575.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OQS60_001800"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OQS60_001800"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS2387576.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17204)
                     /locus_tag="OQS60_001801"
                     /pseudo
     CDS             complement(16219..17204)
                     /locus_tag="OQS60_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17522..18037
                     /gene="zur"
                     /locus_tag="OQS60_001802"
     CDS             17522..18037
                     /gene="zur"
                     /locus_tag="OQS60_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS2387577.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18079..18288)
                     /locus_tag="OQS60_001803"
     CDS             complement(18079..18288)
                     /locus_tag="OQS60_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS2387578.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OQS60_001804"
     CDS             complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OQS60_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS2387579.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OQS60_001805"
     CDS             complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OQS60_001805"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS2387580.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OQS60_001806"
     CDS             complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OQS60_001806"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS2387581.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21059..23482
                     /gene="plsB"
                     /locus_tag="OQS60_001807"
     CDS             21059..23482
                     /gene="plsB"
                     /locus_tag="OQS60_001807"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS2387582.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OQS60_001808"
     CDS             complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OQS60_001808"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS2387583.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="OQS60_001809"
     CDS             complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="OQS60_001809"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS2387584.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25242..>25429)
                     /locus_tag="OQS60_001810"
     CDS             complement(25242..>25429)
                     /locus_tag="OQS60_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387585.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6579 a         6542 c         6089 g         6219 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcgggt
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagtgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccccagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgcagcgctg cataatcaat agatttacca tatatattat ttaatttacc tttgattgaa
    17101 cctctattat ctaatttatc tttgattgca tcttcattat cgaattgatt tgaattgagt
    17161 gttttattaa agtatccagt tgcaccgggt attatcttca acatgattca tctacctttt
    17221 ttgtcgtacc gtgtcctcct tataattctt ttgccatctg gtcactatca aaaatcgctc
    17281 ataatttctg aactacaggt atgcgtttaa acccagttaa ataaatagcg agttaacttg
    17341 aagcaattta tttaaaaatg aattattaat tattatcgtg ttgataatgg tacgacaagg
    17401 caacataaca ccttacctac aaaacagaaa atcaacatta cggcaacaat aagggttctc
    17461 ggtgtttttc ctggaacatg gtaaagtaag gacattctta acccccactt tgaggtgccc
    17521 gatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcgcagcg
    17581 taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc ctgatgagtc tgcaggatgg
    17641 cgctatcagc gcttatgatc tgcttgattt actgcgcgaa gctgaaccgc aagccaagcc
    17701 gccaacggtt tatcgcgcgc tggattttct gcttgagcaa ggttttgtgc ataaggtgga
    17761 atccaccaac agttatgtgc tctgtcatct gttcgaccag cccacgcata cgtcagccat
    17821 gtttatatgc gatcgctgcg gcgcggtgaa agaagagtgt gcagaaggcg tggaagacat
    17881 tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg cataatgtga ttgaagcaca
    17941 tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt catcctgaac agtgccagca
    18001 tgatcactct gtgcaggtga aaaagaaacc gcgttaagag ggcgtacatc cttgtacacg
    18061 tcgggcagga gggattaatt accagcgata ttcattgcgg gtttcccaat ccacgacctc
    18121 tttttctgcc tgatctttct gataaccata acgttcctgg attttaccga ccagttgatc
    18181 acgtttacct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    18241 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    18301 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    18361 tcagcaattt ggcttaggat aatttattca gaatttttaa ccgtcacgtt gcggcaaacc
    18421 aggtaccgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgcaacgcca
    18481 gaaagacggt tagtgccagc cacaaaccat gattacccag ccacggcagc gtaaggagcg
    18541 tcagcgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    18601 cgcctataaa catgccgtcc agcagataac accagacgcc aaccaacggc aaaatcacct
    18661 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    18721 tgatgtgttc ccctgccagc aaataaacca ccgaaaatag taacgctacg atccccgact
    18781 ggcggcacgc tgcccgccag acatccagca actggctacc gtcgcgcgca ccgtatgcct
    18841 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    18901 gtagcgtcat cagaaccgcg tttacagcga taatgtcact ccccagtcgc gcgccaagta
    18961 cggtgatcgc gccgaaacag agttgcaaca acagcgaacg cagcatgata tcgcggttaa
    19021 gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt cgacatttcg ccggaaattc
    19081 cgcgtagttt gaggatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    19141 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc actagccaga
    19201 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    19261 attgcacgcc gagtaaccaa ccgagtaata ccagattcgc cagcgacgcc ggtgcgctta
    19321 accagcggat ttcaagaaag cgccgcgcct gttccaggac tgcttcacta ccgccaacaa
    19381 tatgcagagc cagatcgata atcggtgtac gcagcagcgc aattaacgcc ccagccccca
    19441 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    19501 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacagc ataaagagaa
    19561 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccgggcta tcaagatgac
    19621 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    19681 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    19741 tgatgaagag agatgaaaaa caaaccgcga cgcctggcgg catcgcggtc tcagagatat
    19801 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    19861 gctgacgaag gtcaacgaca attggtttaa actcgctatt ttctggcaac agttcgactt
    19921 tattaccctg ttttttcagg cgcttaacgg taacttcgtc atcaatacgt gcgacaacga
    19981 cctgaccgtt acgtacatcc tgagttttat gcactgccag caagtcacca tccataatgc
    20041 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    20101 aggaaggatc gacctgataa tgaccttcaa tatgctgctg cgccagaagc ggttcaccgg
    20161 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaacaga cgaatcccgc
    20221 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    20281 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    20341 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    20401 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    20461 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    20521 tatccaaaat gcgaccataa caggatgcac caggttatca cggcgacgat aatcgcaatc
    20581 agcaccgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    20641 attcggtcaa ccactgcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    20701 acggagctga taagcagcac gcgggtaatt gcgtccacat ccagccagca ggcgatgacc
    20761 accgccaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    20821 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    20881 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    20941 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    21001 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    21061 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    21121 aagcaagtct attccggcag atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    21181 tatgtacgtt ttaccgtaca actcgaaagc agatttgctg acgttacgcg cccagtgtct
    21241 ggctcatgac ttgcctgacc cgttagagcc gctagaaatc gacggcacgc tactgccgcg
    21301 ctatgtgttc attcacggcg ggccgcgtgt attcacctat tacacgccga aagaagagtc
    21361 tattaagctg ttccacgact atctcgattt gcaccgtagc aacccaaatc tggatgtgca
    21421 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtaaa
    21481 cccgccgctg cgtatgctta acggcgtaca gaaatttttc gccgtactgt ggctcggtcg
    21541 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    21601 cacggataaa actatcgcac agaaactggc gcgcgtggcg cgtatgcact ttgcccgtca
    21661 acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    21721 cgcctcccgc gccattgcca aagcggtaga agatgaagcg cgcagcaaaa aaatctccca
    21781 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    21841 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttatcaggg
    21901 catcaacgtc cataacgccg agcgcgttcg ccagctggcc cacgacggtc atgagctggt
    21961 gtatgtgcct tgccaccgca gtcacatgga ctacctgcta ctttcttacg tgctgtacca
    22021 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggccgg
    22081 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acttttaaag gcaataaact
    22141 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    22201 gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg ctggatccga aaaccggtac
    22261 gctgtcgatg accattcagg cgatgctgcg tggcggcacg cgtccgatta cgctgattcc
    22321 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    22381 cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc ggtttaagca agctgcgtaa
    22441 tctcggtcag ggttacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    22501 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    22561 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    22621 ggcaaacgcc atgaacctgt gttgtactgc gcttctggca tcacgtcagc gctcactcac
    22681 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    22741 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    22801 aatgaacaag tttgaagtcg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    22861 gcaagcggtg ctgatgacct actatcgcaa caacattgcg catatgttgg tgctgccttc
    22921 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    22981 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    23041 gttgccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    23101 gcaagatgat gagttgcata tcaacccggc gcattctcgc cccctacagc tgctggccgc
    23161 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggttgttga gtgccaaccc
    23221 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gttgcgcaac gtctctccgt
    23281 gctgcacggc atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    23341 gacgctgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    23401 gaaggtttat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    23461 tgcgacgcag ggcgaagggt aatcagagag aattgccgga tgcggcgaaa acgccttatc
    23521 cggccttcca taatctgcaa attcaataaa ttgcgatgtg ttgtgtaggc ctgagaagcg
    23581 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    23641 aaatgccagt aactcattgc cagtcctaaa aacagtacca gaccaacata gttattattc
    23701 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    23761 aacagcgcgc cagccaccag aattgaccag taatatcccc agcctaagcc atttaactca
    23821 ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa taccaataat caatttatcg
    23881 tattggccga acaggattgc ggtggattta ataccaatct tcacatcatc gtcacggtca
    23941 accattgcat actgcgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    24001 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    24061 aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc gcttcataaa cgggtacacc
    24121 cacgccaacg ccagagcggc aatcgacaac agaatggtca tcgtattcag cgtcagcacc
    24181 agtaaaaatg aaatcaatac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    24241 gcgccgctgg gaagcggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    24301 gcataatcat tcaccacaca tccggcagcg cgcatcagcc agacacccgc gacaaacacc
    24361 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    24421 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    24481 agcttattct gcgtcagact ccactccata tttttttcct cttagtacaa cggtgacgcc
    24541 ggtaaaaaca gttctgttag caacagcggt ttaccgctta atcgcaggcg ggaacgtcgc
    24601 ccccacagtc cggcatcacg gcctatctca ataaagtccc gggttaatgt cgatgatgtg
    24661 aacagatagc gtcctagcgg cgttttaccc aatttttgta acgccagctc cggcccgctt
    24721 aacgttgaca caggaacgac ggtacgaccg gcaagccacg gttcaccatc ggcacataac
    24781 aaaatttcac gtaaccagta acgagactct ttcggcagca gcggcagttc ttcggggatt
    24841 tcattctgct cgacaaaccc ttcgcggatc atcgtcacgc tcaccgtttt tccctgctgt
    24901 tcaaaacgtt ttgtcatgga atcttccagc aacagccagt cgagcagttg cgaatccagg
    24961 gcagggatct ctttaaaata gcgcagcgca cgcagttgcg ttaacgcggg gtgtgacatg
    25021 ccgaactctc cgttacataa cgtaaaggca ttgtatcgca gaaaagtgtt tacgggagat
    25081 gtaaagcaaa ttaagtgatc taatgcgcaa caaaaacgca acaatcgcat gaagtggtaa
    25141 aacgctgttg aaccttacgc tttgccgatg cccgtgctgg cgagggcaac cgtaggggca
    25201 tcaccgccca tgatgccagg aacggtgatg ttttccttta cttaaatacc tatattcttt
    25261 atacgagtag ctcgccaact cgcagtaaaa attgggaaaa gcgcatattt accatgagat
    25321 ttggagctaa aaagtgtttc ctgagtaaga ttaataggca acatcttatg tctggattta
    25381 tagatacacg tggaattcca attggtatct tttgtaggat tcaacatat
//
LOCUS       DAJVYM010000047        25104 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVYM010000047 DAJVYM010000000
VERSION     DAJVYM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.047"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            194..382
                     /locus_tag="OQS60_001811"
     CDS             194..382
                     /locus_tag="OQS60_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS2387586.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="OQS60_001812"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="OQS60_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS2387587.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="OQS60_001813"
     CDS             1143..1307
                     /locus_tag="OQS60_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS2387588.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="OQS60_001814"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="OQS60_001814"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS2387589.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="OQS60_001815"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="OQS60_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS2387590.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="OQS60_001816"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="OQS60_001816"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS2387591.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="OQS60_001817"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="OQS60_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="OQS60_001818"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="OQS60_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS2387592.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="OQS60_001819"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="OQS60_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS2387593.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="OQS60_001820"
     CDS             14457..15953
                     /locus_tag="OQS60_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS2387594.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="OQS60_001821"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="OQS60_001821"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS2387595.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="OQS60_001822"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="OQS60_001822"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS2387596.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="OQS60_001823"
     CDS             18047..20107
                     /locus_tag="OQS60_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS2387597.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="OQS60_001824"
     CDS             complement(20289..21611)
                     /locus_tag="OQS60_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS2387598.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="OQS60_001825"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="OQS60_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS2387599.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="OQS60_001826"
     CDS             complement(23084..23983)
                     /locus_tag="OQS60_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2387600.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="OQS60_001827"
     CDS             24503..>25104
                     /locus_tag="OQS60_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS2387601.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAJVYM010000048        24802 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVYM010000048 DAJVYM010000000
VERSION     DAJVYM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.048"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..796)
                     /locus_tag="OQS60_001828"
     CDS             complement(<1..796)
                     /locus_tag="OQS60_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS2387602.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYL"
     gene            1363..1728
                     /locus_tag="OQS60_001829"
     CDS             1363..1728
                     /locus_tag="OQS60_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2387603.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1768..2424
                     /locus_tag="OQS60_001830"
     CDS             1768..2424
                     /locus_tag="OQS60_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS2387604.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2491..3909
                     /gene="dcm"
                     /locus_tag="OQS60_001831"
     CDS             2491..3909
                     /gene="dcm"
                     /locus_tag="OQS60_001831"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS2387605.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3890..4360
                     /gene="vsr"
                     /locus_tag="OQS60_001832"
     CDS             3890..4360
                     /gene="vsr"
                     /locus_tag="OQS60_001832"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS2387606.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4349..5270)
                     /gene="yedA"
                     /locus_tag="OQS60_001833"
                     /pseudo
     CDS             complement(4349..5270)
                     /gene="yedA"
                     /locus_tag="OQS60_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5443..6360
                     /locus_tag="OQS60_001834"
     CDS             5443..6360
                     /locus_tag="OQS60_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS2387607.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6439..6621
                     /locus_tag="OQS60_001835"
     CDS             6439..6621
                     /locus_tag="OQS60_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS2387608.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6792..8486
                     /gene="yedQ"
                     /locus_tag="OQS60_001836"
     CDS             6792..8486
                     /gene="yedQ"
                     /locus_tag="OQS60_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCS2387609.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8483..9298)
                     /locus_tag="OQS60_001837"
     CDS             complement(8483..9298)
                     /locus_tag="OQS60_001837"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCS2387610.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9596..9823)
                     /gene="yodD"
                     /locus_tag="OQS60_001838"
     CDS             complement(9596..9823)
                     /gene="yodD"
                     /locus_tag="OQS60_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS2387611.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9986..10174
                     /gene="dsrB"
                     /locus_tag="OQS60_001839"
     CDS             9986..10174
                     /gene="dsrB"
                     /locus_tag="OQS60_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS2387612.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10218..10841)
                     /gene="rcsA"
                     /locus_tag="OQS60_001840"
     CDS             complement(10218..10841)
                     /gene="rcsA"
                     /locus_tag="OQS60_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS2387613.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11126..11911)
                     /gene="fliR"
                     /locus_tag="OQS60_001841"
     CDS             complement(11126..11911)
                     /gene="fliR"
                     /locus_tag="OQS60_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS2387614.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11920..12189)
                     /gene="fliQ"
                     /locus_tag="OQS60_001842"
     CDS             complement(11920..12189)
                     /gene="fliQ"
                     /locus_tag="OQS60_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS2387615.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12199..12936)
                     /gene="fliP"
                     /locus_tag="OQS60_001843"
     CDS             complement(12199..12936)
                     /gene="fliP"
                     /locus_tag="OQS60_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS2387616.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12936..13301)
                     /gene="fliO"
                     /locus_tag="OQS60_001844"
     CDS             complement(12936..13301)
                     /gene="fliO"
                     /locus_tag="OQS60_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS2387617.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13304..13717)
                     /gene="fliN"
                     /locus_tag="OQS60_001845"
     CDS             complement(13304..13717)
                     /gene="fliN"
                     /locus_tag="OQS60_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS2387618.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13714..14718)
                     /gene="fliM"
                     /locus_tag="OQS60_001846"
     CDS             complement(13714..14718)
                     /gene="fliM"
                     /locus_tag="OQS60_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS2387619.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14723..15187)
                     /gene="fliL"
                     /locus_tag="OQS60_001847"
     CDS             complement(14723..15187)
                     /gene="fliL"
                     /locus_tag="OQS60_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS2387620.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15292..16419)
                     /gene="fliK"
                     /locus_tag="OQS60_001848"
     CDS             complement(15292..16419)
                     /gene="fliK"
                     /locus_tag="OQS60_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS2387621.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16416..16859)
                     /gene="fliJ"
                     /locus_tag="OQS60_001849"
     CDS             complement(16416..16859)
                     /gene="fliJ"
                     /locus_tag="OQS60_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS2387622.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16878..18251)
                     /gene="fliI"
                     /locus_tag="OQS60_001850"
     CDS             complement(16878..18251)
                     /gene="fliI"
                     /locus_tag="OQS60_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS2387623.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18251..18937)
                     /gene="fliH"
                     /locus_tag="OQS60_001851"
     CDS             complement(18251..18937)
                     /gene="fliH"
                     /locus_tag="OQS60_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS2387624.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18930..19925)
                     /gene="fliG"
                     /locus_tag="OQS60_001852"
     CDS             complement(18930..19925)
                     /gene="fliG"
                     /locus_tag="OQS60_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS2387625.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19918..21576)
                     /gene="fliF"
                     /locus_tag="OQS60_001853"
     CDS             complement(19918..21576)
                     /gene="fliF"
                     /locus_tag="OQS60_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS2387626.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21792..22106
                     /gene="fliE"
                     /locus_tag="OQS60_001854"
     CDS             21792..22106
                     /gene="fliE"
                     /locus_tag="OQS60_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS2387627.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22440..22772
                     /gene="emrE"
                     /locus_tag="OQS60_001855"
     CDS             22440..22772
                     /gene="emrE"
                     /locus_tag="OQS60_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS2387628.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22941..23492
                     /gene="ybcL"
                     /locus_tag="OQS60_001856"
     CDS             22941..23492
                     /gene="ybcL"
                     /locus_tag="OQS60_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS2387629.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23502..24299
                     /locus_tag="OQS60_001857"
     CDS             23502..24299
                     /locus_tag="OQS60_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2387630.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6098 a         6130 c         6347 g         6227 t
ORIGIN      
        1 caagataaac attgttggcg tcgtattttg caccaaatgt ccatgcttca gctgtatcac
       61 caccagcaaa tttgctgtat tcattgtagt gatacccgcc aagagctact tgagaattgg
      121 tacggtcaga gtttgagtaa gcggcaccta agctcaaacc gaagtcaaag tcatatgaag
      181 tggaaagacc aaaaccatca ccgttctgga agcgaacgtc ttcgtgacca tttttagtac
      241 cttcttgatc ttctcctgca ttgctgcctt cgttgttacc ttgatactgc aatgcaaagt
      301 tcagaccttc caccagaccg aagaaatcac tattgcggta agtagctacc ccatttgcac
      361 ggcctgccat gaagttatca gtccaggtat aggagtcgcc gccgaataca ggaagcatat
      421 ctgtccaggc ttcaacgtcg taaatcacac catagttacg accatagtca aaggagccca
      481 tctcgccgaa cttcagacct gcaaaagcaa gtttgacata agtattatca gaaccttcag
      541 tgtcgctagc ctttgttttg gtcagtgctt gaccataacc agttaactga tctgtaattt
      601 gggtctcacc tttgaagcct aactcgatga tagtttcatc gccgtcatcg ccagggttat
      661 cggaaaaagt atgacgagca tcaacttttc cgtacagatc cagtttattg ccatttttat
      721 tataaatttc agccgcattt gctgcgccag caactaataa cgctgggacc agcattgcca
      781 gaacttttct tttcattatg tattcccttg tgattataat cttcatgaat atatcaataa
      841 gtgccgttat ccaaaaaaaa agcacatttg gatactattc tatgaagttc attttatttt
      901 aaagattgcg tgtaacaaat gtatttaaaa gatttcaaaa tgtttcttat ctatttgata
      961 taattcagaa ttatgaattc taaatataag catccatgaa aattataaat gtatatcaat
     1021 atgttatgag ttgaatggaa agatagggag tatcaatgat tgttgcgcca aacggcaggc
     1081 gtgatcacgc gcacattaat gaaggtataa aaaaagccgg catcatgccg gcccaaaaaa
     1141 tataaacaag acgataaaag gctaataacg gggttatcat gacacagaaa tcaaaaaagg
     1201 tataattgta ttcagtgcta atatattgtt tgggtgaatt aataaaacta ttaccaagga
     1261 tataaataaa tctttattgc tgtttttcca gtaattattg ccgttgcgca aaatttatct
     1321 atttgttcaa aaaatgattg agtcttgact ggctcatcca atgtggaaaa atgtgacttt
     1381 tatcacataa tagtactaag tctgaatttt ccgggttatc tcaaaatgga atacggttcg
     1441 acaaagatgg aagagagact ctctcgcagc cctggcggca aattggcttt gtgggctttc
     1501 tatacatggt gtggctattt cgtctgggca atggcgcgtt acatctgggt gatgagccgg
     1561 atccctgatg ccccggtgtc gggatttgaa agcgatttag gctctaccgc gggaaagtgg
     1621 ttaggggcac tggtcggatt tttatttatg gctctggtcg gggcattgtt aggcagcatt
     1681 gcctggtaca cccgacctcg tcccgcacgt agcagacgct atgaataaaa actgctaagg
     1741 tggcgactct ttggcaacgg agcgcgtatg gacttacaac actggcaggc acagttcgaa
     1801 aactggttga aaaaccatca tcagcaccag gacgcggcac agaagctggc ggcagatgac
     1861 gatgtcgata tgctggtgat tttaaccgcc tgttattttc acgatattgt gagcctggcg
     1921 aaaaatcatc cgcaaaggca gcgttcttca atcctggcgg cagaagagac gcgtcgcctg
     1981 ctccgtgaag agttcgagca atttccggcg gagaaaatcg aggccgtttg tcatgccatt
     2041 gccgctcaca gtttcagcgc gcaaatcgcc cccttaacaa cggaagctaa aatcgtccag
     2101 gatgcggatc ggctggaagc cctgggggcg attggcctgg cgcgcgtgtt tgccgtttca
     2161 ggggcattgg gcgtggcgct gtttgatggt gaagatccgt tcgcacagca tcgcccgctt
     2221 gatgataaac gctacgcgct ggatcatttc cagactaagt tgctgaagct accgcaaacc
     2281 atgcaaaccg caagaggcaa gcagctggcg cagcacaacg cgcatttttt agtcgagttt
     2341 atggcgaagc tcagtgccga actggcgggg gagaatgaag gtgtcgatca caaggtgata
     2401 gatgcgtttt cacccgcggg ataagcgcgt ggccctaaat ggctgtaatt atgttaacct
     2461 gtcggccatc tcagatggcc ggttaaatct atgcaggaaa atatatcagt aaccgattca
     2521 tacagcaccg ggaatgccgc acaggcaatg ctggagaaac tgctgcaaat ttatgatgtt
     2581 aaaacgctgg tggcgcagct taatggtgtg ggtgagaatc actggagcgc ggcaatttta
     2641 aaacgtgcgc tggcgaatga ctcggcatgg caccgtttaa gtgagaaaga gttcgcccat
     2701 ctgcaaacgt tgttacccaa accatcggca catcatccgc attatgcgtt tcgctttatc
     2761 gatctatttg ccggaattgg cggcatccgt cgcggttttg aatcgattgg cggacaatgc
     2821 gtgtttacca gcgaatggaa caaacatgcg gtacgcactt ataaagccaa ccattattgc
     2881 gatccggcga cgcatcattt taatgaagat atccgcgaca tcaccctcag ccataaagaa
     2941 ggcgtgagtg atgaggcggc ggcggaacat attcgtcaac acattcctga acacgatgtt
     3001 ttactggccg gtttcccttg tcagccattt tcgctggctg gcgtatcgaa aaagaactcg
     3061 ctcgggcggg cgcacggttt tgcctgcgat acccagggca cgctgttttt tgatgtggta
     3121 cgcattatcg acgcgcgtcg tccggcgatg tttgtgctcg aaaacgtcaa aaacctgaaa
     3181 agtcacgacc agggtaaaac gttccgcatc atcatgcaga cgctggacga actgggctat
     3241 gacgtggctg atgcagaaga taacgggccg gacgatccga aaatcatcga tggtaaacat
     3301 tttctgccgc agcaccgtga acgcatcgtg ctggtgggtt ttcgtcgcga tcttaatctg
     3361 aaagccgatt ttactctgcg tgatatcagc gaatgtttcc ctgcacagcg agtgacgctg
     3421 gcgcagctgt tggacccgat ggtcgaggcg aaatatatcc tgacgccggt gctgtggaag
     3481 tacctctatc gatatgcgaa aaaacatcag gcgcgcggta acggcttcgg ttatggaatg
     3541 gtttatccga acaatccgca aagcgtcacg cgtacgctgt ctgcgcgtta ttacaaagat
     3601 ggcgcggaaa ttttaatcga tcgcggctgg gatatggcca cgggtgagaa agattttgac
     3661 gatccgctga atcagcaaca tcgtccacgt cggttaacgc ctcgggaatg cgcgcgctta
     3721 atgggttttg aagcgccggg agaagcgaaa ttccgcattc cggtttcgga cactcaggcc
     3781 tatcgccagt tcggtaactc ggtggtcgtg ccggtctttg ccgcggtggc aaaactgctt
     3841 gagccaaaaa tcaaacaggc ggtggcgttg cgtcagcaag aggcacaaca tggccgacgt
     3901 tcacgataag gccactcgca gcaaaaatat gcgcgcgatt gccacgcgtg atacggcgat
     3961 tgagaagcgc ctcgccagtc tgttaaccgg gcagggcctg gcgtttcgcg ttcaggacgc
     4021 cagtctgccc ggaagcccgg attttgtcgt tgatgaatat cgctgcgtga tatttaccca
     4081 tggctgcttc tggcatcatc atcactgcta tctgtttaaa gtgcctgcga ctcgaaccga
     4141 gttctggctg gagaagatag gtaaaaatgt tgaacgcgat cgccgcgata tcagtcgttt
     4201 gcaggaactc ggctggcgcg tactgattgt ctgggagtgt gcattacgtg ggcgcgagaa
     4261 gctgacggat gaagcgctta ccgagcgtct ggaagagtgg atctgcggcg aaggtgccag
     4321 cgcgcagatc gacacgcagg ggattcattt actcgcttga tgcgtcctga ataactggcg
     4381 caactacggg ttttgccggg aagagatatt ttcccaacgt gaccagtacc accgcgaaga
     4441 caattacgcc gagcgccagc cattcaatct tcgacagcgt ctctccgccc agtcccgtac
     4501 ccagcaagac cgcgaccacc gggttaacgt aggcgtagct ggtggcgaga gccggactga
     4561 cattacggat taaatacata taagcgttga tggcgataat cgaaccaaac agcgccagat
     4621 agccgaccgc aaggaagcct gaaagggaag ggagcgccgt cagtttttca cctgcaatca
     4681 tcgacgcgat cattaacacc acgcctgccg ccagcatctc aatcgcaccc gccatcatcc
     4741 ctacaggtaa ggtaatgcgc gagccataaa ccgagccaaa cgcccagcta atcgagccga
     4801 ttaaaatcag aatcgcgccc cacggattgc cgcttaaatt tccaccgcta ttgagcatga
     4861 tgattccggc aagcccaatg gcgataccca cccattccag tttgcgcgtt ttaatgccaa
     4921 acaggcggct gaagcaccag ggtaaagagg ggcacggttg caaccacaac ggcggcgatg
     4981 ccggaaggaa cattttgatg ctcggcaacc gtcaccatgc cattaccgac agccagcaat
     5041 aacaggccaa tcagcgcggc attgagcagc ggacgtagcg gggggagctt gtgtccgcgc
     5101 agtagcaaaa atgccatcaa taaaataccg gctgccagga atcgaacgcc tgccatcatt
     5161 aacggaggcc agctttccac gccaatccga atgacaaaat aggttgagcc ccaaatgata
     5221 tacaacgcaa acagcgcgcc aaaaagcggt aacaactggc ggaaacgcat aatccctcac
     5281 ggtggaaata aaaaggtggt tcatagtaaa cgtgaaaatc attctgctgg cgagagatat
     5341 aattgcactt gattgtaaaa aaaatgttga cctgtgaagc aagtatcaga gctgccgttt
     5401 ttgcttcata cttacaccct tcaacaataa aaatgagagg gaatgctttt ggccgggagt
     5461 agtttactga cgttgctcga tgatatcgcc acactactgg acgatatctc cgtgatgggc
     5521 aaactggcgg cgaagaaaac cgccggtgta ttaggggatg acttatcgct caatgcgcaa
     5581 caagtttcag gcgtgcgggc caaccgggaa cttcccgtgg tctggggcgt ggcgaaagga
     5641 tcgctgatta ataaagtgat tctggtgccg ctggcgctga tcatcagtgc gtttatcccg
     5701 tgggcgatta cgcctctgtt gatgattggt ggcgcgtttc tctgctttga aggagtagag
     5761 aaagtgctgc atatgctgga ggcgcgtaaa cataaagaag atccggcgca gagccagcag
     5821 cgtctggaga agctggcggc gcaggatccg ctgaagtttg aaaaggacaa aataaaaggg
     5881 gcgattcgta ccgattttat attgtctgcc gaaatcgtcg ccatcacgct ggggattgtg
     5941 gccgaagcgc cgttgcttaa tcaggtgctg gtgctttcag gcatcgcgct ggtagtgacc
     6001 gtgggcgtct acggtctggt aggggttatc gttaagattg atgacctggg gtattggctg
     6061 gcggaaaaat ccagcgcgct gatgcaggca ttaggtaaag ggttattgat cattgcgccc
     6121 tggttgatga aggcgttatc gattgtcggc acgctggcga tgttcctcgt cggcggcggt
     6181 attgtggtac atggtattgc gccgctgcat cacgccattg aacatttcgc cgggcagcaa
     6241 agtgcagtgg tggcgatgat attaccgact gttttaaatc tgattcttgg atttatcatc
     6301 ggcggcatcg tggtgctggg agtgaaagcc gtagcgaaaa tgcgcggtca ggcacattaa
     6361 tactcgtaca ggctattatg caaaattcgc catcttcaac taaggtgaac aagtttcact
     6421 cgaaaaaagg aggcgagtat gagctttatg gttagtgagg aagttacggt taaagagggc
     6481 ggcccgcgga tgattgtcac tggatactcc agcggtatgg ttgagtgtcg ttggtatgac
     6541 ggttacgggg tcaagcggga agcttttcat gaaaccgagc ttgttccggg ggaggggagt
     6601 cgttctgcgg aagaagtttg aaagggtatc attaacacca ggccggatca cttccggcct
     6661 ggtgtgccaa tgcctgcaat gaatgacagt cgcgtcttat catgccacca ttataaaatt
     6721 atcgctgttt tgcggtacgc tatcgccagg ttatgccaga atcataaaaa agcaggttgg
     6781 gagtcgtcag ggtgcagcac gagacaaaaa tggaaaacca gagctggttg aaaaaactcg
     6841 cacgccgcct ggggcctggt catgtcgtta atctctgctt tatcgtggta ttgctttttt
     6901 ccaccttgct gacctggcgt gaagtggtag tgctggaaga tgcctatatc tccagccagc
     6961 gtaatcatct ggaaaacgta gccaacgcgc tcgataagca tttgcagtat aacgtcgaca
     7021 aactgatctt tttgcgtaat ggcatgcgcg aagctctcgt agcgccactg gatttcactt
     7081 cactgcgtaa tgctgtaacc gagttcgaac agcatcgcga cgagcacgcc tggcaaattg
     7141 aactcaaccg acgacgcacc ctgtcagtca atggcgtatc ggatgcatta gtcagcgagg
     7201 ggaatctcct gtctcgcgaa aatgaaagcc tcgacaatga aattaccgct gcactggaag
     7261 ttggttactt gctgcgactg gcgcacaaca cctcgtcgat ggttgaacag gcgatgtatg
     7321 tctcgcgtgc cggattttac gtttcgacgc agccgacctt gtttacgcgc aatgtaccaa
     7381 cgcgttatta cggctatgtc acccaaccct ggtttatcgg ccattcgcaa cgagaaaatc
     7441 gtcaccgcgc ggtacgctgg tttacttcgc aaccggagca cgccagcaat actgaaccgc
     7501 aggttaccgt cagtgttccg gtagacagta ataactactg gtatggcgtg ctggggatga
     7561 gtattcccgt gcgtaccatg cagcaatttt taagaaacgc catcgataaa aacctcgatg
     7621 gtgagtatca gctctatgac agtaagctga gatttttgac ctcttccaat cctgaccatc
     7681 caacagggaa tatttttgat cctcgtgaac tggccttgct ggcgcaggcg atggaacatg
     7741 acacgcgggg cggcattcgt atggacagtc gctatgttag ctgggaacgt ctggaccatt
     7801 tcgacggtgt gctggtgcgc gtccatacgc taagcgaagg cgtgcgcggc gatttcggca
     7861 gtatcagcat tgcattaacc ctgctgtggg cgctctttac caccatgtta ctcatctcct
     7921 ggtatgtgat tcgccggatg gttagcaaca tgtatgttct gcaaagctcg ttgcagtggc
     7981 aggcgtggca cgacacctta acccgtttat ataatcgtgg cgcactgttt gaaaaagccc
     8041 gtccgctcgc gaaattgtgt cagacgcacc aacatccttt ttctgtcatt caggtcgacc
     8101 ttgaccattt taaagcgatt aatgaccgct ttggtcatca ggcgggcgac cgtgttcttt
     8161 ctcatgctgc cggattaatt agcagttcct tgcgtgcgca ggacgttgcc gggcgggtcg
     8221 gtggtgagga gttttgtgtg attctgccag gcgcgagtct gacggaggct gcggaagtcg
     8281 cagaacgtat tcgcctgaag ttaaatgaaa aagagatgtt gattgctaag agtacgacga
     8341 tacgcatcag tgcctcgttg ggggtaagta gcagcgagga aaccggtgat tatgattttg
     8401 aacaactcca gtcactggct gaccgtcggc tttatctcgc taaacaggcc gggcgtaatc
     8461 gggtatgcgc gagcgataac gcttaacgag cggagaaaaa atggtccagc ccttcacgcc
     8521 agccttccgg tccttcacgc tgcgttcgcc agacgcgggt cggatcctca tcatgcagat
     8581 gcaccccttc acggtttagc cctttcacaa tcaccgcgta atccattacc tccagtaagg
     8641 gcgcatcgtt tggcccatcg cccaggccaa gtgtggttgg gcgtttgcct gataattgtt
     8701 gataggtcgc gataatccag ttggcggcct gatcttttcc ggcagaggca tccaggacgt
     8761 gccagaagcg cgcaccctgc ataaactgta agcccagttc gttcagacga gcggtaaatt
     8821 gtgccatacg ctcgtcactg tcgcgccaga ttagcgttac cgacgcctca tgcagctgcg
     8881 tcagcgccgc ctggctacgg cttaatcccg tccattcggc gatggttgca tcgtcgacat
     8941 catcgaaagt cgtgaattta aaatgttctt tctcgcgtag cgtatttaaa acctggctga
     9001 tttcgccatg gctaatacct gagatgatgc gtggaaaacc gtctatatcc tgccattgtt
     9061 cagcaagctg gatcactgcg ccattttcgg caatcagtgg taacccttgt agccccaacg
     9121 ttttttgcaa gtacagcatt tccgctgaag ttttactgct acagagaatg acgggaacat
     9181 tcgcttcgcg taaacggctg agccagaggg cagtcggttg ccagtcataa ctatgactgt
     9241 ccagcagggt gccatcaaga tcgctaaaaa ccagtagtgg ttgttgaatt gaaaacatgg
     9301 tcgtgcagtt ctccccttac caggatgatt ctcaaaacag cacaaataat caaagatgat
     9361 tttttcggga gcgatcccgt tgacgccaga gccaccaagg ggtgacgcac gtcacatttc
     9421 tattcataag tagcgttaat tattcatatc gcgaatattt tcttgtcagc gaaaaaaatt
     9481 gcggataagg tgatgaacac atcagatttc ctggtgtaac gaatttttta agtgcttctt
     9541 gcttaagcaa gtttcatccc gaccccctca gggtcgggat ttttttattg tgcattcaac
     9601 gattcacttc agagacgctg aagtcatgaa tatccagttc gaaggcatcc gccaattcac
     9661 gccatgtatg atattcacgt ccatcgacgc taacgtgttc agaatcgttc tggctgcgat
     9721 gaacttcgct ttcgctaatt tcattgatcg ccgccagcag ggcatcgaca tcgacgctga
     9781 cctcacgttt tgcggtatcg ctgtactctt ttgcggtttt catcatcttt cctcgcaacc
     9841 gttactctgt tgttgccgcc agtcgttaag tccgcctggc atctcttaag tatagaccgt
     9901 cgagaaaatc agcactcgcg cgcgcctgga gctgccagaa aaacgaaatt gttctacact
     9961 ggcacaaagc cacaggagga aaacgatgaa ggtgaatgat cgggtaacag tcaaaacgga
    10021 tggcggtccg cgtcgtcctg gcgtggtact ggcagttgag gagtttagtg aaggcacaat
    10081 gtacctggtt tcgctggaag actacccgct cggcatctgg ttctttaatg aagcagggca
    10141 tcaggacggt atctttgtgg agaaagcaga gtaatcttat tcagcctgac tggtgggaaa
    10201 ccaccagtca gaatgtgtta gcgcatgttg acaaaaatac cattagtcac attatccgtc
    10261 agtcggacga catggtagat aacctgttta ttatgtgttt tgatcttacg tttaatatta
    10321 cctttatgcg atgaaacggt cttggctttg atattcattt ggtcagagat ttgaatggtt
    10381 ccctgacctg ccatccacat tcgcaacata ctcgattcgg ttcggctcaa tgataacgtc
    10441 ggcatattta aaaaagaggt tatcgttgtc tcttttttca gaatatcgcc aaggatatcg
    10501 tcgagagatt ccggtttaat cgatttagaa cttatcaata atttttttct gaccaataga
    10561 tattcatcaa aatgaacatt ggcaattgcc ataaaaacga taaataacgt attgggatgt
    10621 tgattaatga tgtgcttgat gtgctgactg ttagaagcat catggatgaa acagtcctca
    10681 ttaataaaca ccactgaagg gcgctgtgaa tcacaagcta tggcaaggtc atcaacggtt
    10741 tcaatgtcgt tgatttctct ttttttaacc cctctactca acagataccc ggttaaacct
    10801 agtcgggtgt aactacataa atccataata atcgttgaca tggcataccc tcactcaatg
    10861 cgtaacgata attcccctta cctgaatatt tcatcatgac taaacggaac aacatgggtc
    10921 acctaatgcg ccactctcgc gatttttcag gcggacttac tatcccgtaa agtgttgtat
    10981 aatttgcctg gaattgtctt aaagtaaatg ttgcgatatg tgagtgagct taaaacaaat
    11041 atttcgccgc aggagtatcc tggaagatgt tcgtaggtat tatttttcgg ataatcctta
    11101 ggataacatg ataaacgtta cggaattata ttaatggcaa ttcactaata atatcagcca
    11161 gcaaattaaa catctcactg aataaatgtt cgcaaaaagg tgcaattaac ggcattaatg
    11221 ccgccattaa agagataccg acagtcagag ttaatggaaa tccaataaca aaaatggata
    11281 attgcggggc catacgattc agtaaaccta atgccagatt cagggtcagc agcagagtaa
    11341 tgagcggtaa cgccagcatc agcccgttaa ggaaaatcaa actccccgct ttggtgagtg
    11401 ccagaaacgc attgctgttc aacggttcgc caccaattgg cagggtgtga aaggtatcga
    11461 ccagcagtga aatcaaccat aaatgaccgt taaatgtcag gaacagcagt aacgccagca
    11521 tatccatgat acgcgctaaa acgggcatat tgagatggct ggccggatcg acaaacgtcg
    11581 caaatgacag ccccatttgc agaccgataa tttcgccagc ggttcgcaca gcggcaaagg
    11641 caaattgcat ggtaaaacca agcgcaatgc cgatcaggat ctgctgcacg gccagccaca
    11701 gagcaaagaa cgaaaaaaca ggaacatcgt tggcaggtaa tgatggggca atgacgaacg
    11761 tgatcatcat tgccagcccc agttttaccc gtttcggcac gctgcgttcg ctcagaattg
    11821 gtgcggtaga aatgagcgcc agcacgcgca gtaacggcca gaagtacagg cttaaccagg
    11881 aaagccattg atcgcttgtc acctgcatca ttgtacggcc tacccgatga tatacggcag
    11941 gttagtgaac aaggtgcgga cgtaatccag caacagattg agcatccacg gtccggcaat
    12001 aataatggca ataaatacgg cgatgatttt tggaataaac gacagcgtca tttcgttgat
    12061 ctgcgtggcg gcctgcaaaa tactgatgat aaggcccgtg accagcgcca ccagcaacag
    12121 cggggcagcc agtgccagcg cgactttcat cgcttcagtc cccatcatca tgaccgattc
    12181 aggtgtcatt ttgcctctct agctgtaaaa gctctgcgcc agcgaaccga ccagcaattg
    12241 ccagccatcc accagtacaa acagcatcag tttaaagggc agagcaatgg tggcgggggg
    12301 gaccatcatc atccccaatg ccatcaacac gctggcaatc accaggtcga taatcaaaaa
    12361 aggaatgaaa atcgtgaagc ctatctgaaa tgcggttttc aattcgctgg tcacgtaggc
    12421 cgggagcaaa atgcgcatcg gcacggcttc aggtccctgc aacgggccgg tattcgctag
    12481 tctggcaaac aaccctaaat ctgcctcacg ggtctgacgc agcataaact cacgtagcgg
    12541 ctgcgcccct ttttccagcg cctcctgcat tgatattttc tcttcgctga atggctggta
    12601 cgcatcgaca taaattttgt cgatcaccga tgacataata aaaaaggtca aaaatagtgc
    12661 cagccccagc aatacctggt taggtggcgc ggagggcgtt cccagcgcgt tacgcaataa
    12721 accaaaaaca atgatgatgc gggtgaaact ggtcatcatc agtaaaattg ccggaataaa
    12781 cgtcaacgag gtgatgaaca ccagcgtctg caccgggagc gaccagcttt gtccaccgcc
    12841 aggcagcggc tggctggtga tacccggcag ttgcgcgaag gcgaggggcg taaccagcca
    12901 gagaaggaca ggtgcgacag acaacaaacg acgcatcagg atctcccact acgcttaagc
    12961 aaatttttca tgaccgactg aaaatcggtc tgcggtatct cttctgttgg tgctgaaggg
    13021 ggaagtttat gcagcagatt gatttgccct gcggtaacgc cgagcaccag ccgtgcatct
    13081 tccacatcga ccaccacaac ccgttcacgc gcgcccagcg aagcactggc gctaattttc
    13141 agaccgttaa cgccagtgcg tttcggggca aatcccaacc gttttaccag ccaggcagca
    13201 gcgaggatca gggcaataat ggcgatgagt gcgccgctga cctgcagcag tggcgcagca
    13261 gaaaccggcg tggaagattg cacagtagcg tggttattca tcactaacgg ctcaggcgac
    13321 gcattcgctc agacggagta atgatatcgg tgatccgcac gccatattta tcggcaacga
    13381 ccaccacttc gccctgggag attaaataac cgttgatcag aatatccagt ggttcgcccg
    13441 ccagaccgtc cagcgccacg acggaccctt gcgtcagacg caacagctct ttgatggtca
    13501 tccgcgtacg gcccagctcg acggtcagct tgaccggaat atccattatc aggtcgatat
    13561 cctgcaacgt tccgctgaca tcaccaccgc caaattgctg gaacaccgcg tcggcagcgc
    13621 ttttgctgct ggttgatttt tgttcgctca acgcttcagc ccacagatcg tccattgcgc
    13681 cgttgttgtc atcggccgga ttattcatgt cactcatttg ggctgttcct cgttcagaga
    13741 atttaaaatc gggttaatca aatgttctat ccgtaacgca tactgaccgt tgagagtgcc
    13801 gtactgactg gtcagcaccg ggacgccgtc aacatgggcg atgatgccat cgggtttttc
    13861 tatcggcagg acgtcgccgg gtttcagttt taaaatctgc gacaggcgta gcgagatatc
    13921 ggcaaagttg gcgaccagct ccagctgtga atgctgcacc tggcgcacca ggttatcgcg
    13981 ccagttctga tcttcattac gcgagttttc cagcggcggg ttaaccaaca attcccgtag
    14041 cggctcgatc atgctgaatg gcagacagat attaaattcg ccggtcaggt tgccaatctc
    14101 cacatggaac ggcgtgttaa ccacaatgtc gttcggcgag gtggtgatat tggtaaattt
    14161 cacctgcatt tccgaacgca cgtactcaac ttccagcgga ttaatcgcct tccaggcgtc
    14221 gctatagcct tcaagcgcca gctttaacat gcggttgatg acgcgctgtt cggtatgggt
    14281 aaactcgcga ccttccactt tggtcgggaa gcgtccattg ccgccaaaca ggttatccac
    14341 ggcgataaac accagactcg gtgaaaacac caccagccct gtgccgcgca gcggtttcag
    14401 atggataagg ttcaggttgg tcggcaccgg caggttgcgg gcaaattcat ggtacggctg
    14461 aatgcggatg gccccgacgg ttatatccgg gctacgacgc agcaggttga acagccccat
    14521 acgaaaatgg cgggcaaagc gctcattaat gatttccagc gcctgcaaac gttcgcgcac
    14581 aacccgtcgt tgggtattcg gatcgtacgg acgaatgtca ctttcgccgc taacactggc
    14641 tgtcggttcg tctttgactt cgctgtcacc attcaacagc gcatcaattt cagcttgaga
    14701 aagaatacta tcgcccatgt cgttatcgca gaataaaagc ggtatacatc acgtcggtga
    14761 catcctgttt cggttgcccg gcaacaagcg gggtggaaag cgtggtttta atctcggcaa
    14821 tcaggttttt cttgccttct tctgtcgcca gtacggcagc atcctgacgc gaaaacagta
    14881 acagcaaacg gctacggact tccggcaaat actcactcag ccgcgagcgg gtagcttcat
    14941 ctttgagtcg cagggttata ccgatataaa gcacgcgatc cgcatcgccc aaattgaccg
    15001 taaaggtatc cagcgcatag aagaccggtg agggcacgac gcgatgctgc gctttgtctt
    15061 cagcggcaac ctgatgcgaa tgccagtagc tgtaacctgc gctggcacag gccgcgaggg
    15121 taatgaatac cagaatcggg agccaaagcg atcgcttgct tttcttgctt atcgcgtaat
    15181 cagtcatgtg ttgcggtctt cctgtgtcgc tactgcttat cggctaatta tcctgcgtct
    15241 tgagcgcaac aaagcgggga aaagacgcgg attacggtgc tacctctgac gttaggcgaa
    15301 aatatcaacg ccgctgttgc ctgttacgcg cccttgtaaa gagacgggaa ccggaagcgt
    15361 atcgtcttct tcccccgcca gaggttcatg gtttactgtg cgttggcttt gctgttgttg
    15421 ggaagcggcc tgctgctgac cactaaagct ttcgccactg atgttgcttt gccctaactg
    15481 aatgccactc tcggccagct gcgtacgcag taccggcagc gctgcttcca gggcggcgcg
    15541 tacgtgctga tgcggcgaaa ccatctggat ttgcgcctgg ttatcatcca ctttgaggga
    15601 gatttgcact tcacctaaat cctgcgggtg cagacgcaac tctgcacttt gttgtccctg
    15661 gcgggtgaac agcgaaatat gctggcttaa tgattgttgc cattcgtgag aacccagcgg
    15721 tgcactcaag acaggcgcgg cgacggtgag cagtggctgt gtctggtgtg gagtgattag
    15781 cgggctggcg gcagcggtca ccggtgaagg tgtgctgatg acttccgctt tactctgggc
    15841 ttctgctacc agcggtgtta atggctgagc tggcgtgccg ggggcatcat caggctgtgc
    15901 tgttgtgagt tgcgcagaag tcagttttgt gaagagcgtt ggtttctctg ccggtaacac
    15961 ggttgacggc gcatcagtca ctttgggcgt attgtcaaaa cccggcaaca tcgcaaaaag
    16021 ggcgctcagg cttgcggtga ggtcttcatt cagatcatcc gctttttcgt cttttgtcgt
    16081 atttttgtca gccaccgcag ccaacgtcat cgtatgagcg gtggttaacg gtgtcgatgt
    16141 ggattgttcg acgttgatga caggcagtgt ttcatccaca gggatcagta aatccgcttg
    16201 ttgcgcgtcg gaaacaatat cgctgatcag cagctcgcct tttgtcgtgg gcttatctgt
    16261 tgccaccagc acctgggggg ccgctttgtc ggtagttgtc tcgcttgcta atgcttcgct
    16321 caacaacgcg agaaaatctt gtgcagcatc gctggctttg ccgccaggca atgtggtggt
    16381 gtcaacgtcg gcggtaatca agggcgctaa gcgaatcatt caggtttcct catggcggcg
    16441 cgctgggcga actcatccat ctttttctga tcgaggcggt tttctgcaag cagtgccgcc
    16501 gtggattgcc gttcctgcag tgtctgccag gcctgcaaac gttgtttctt ttctcgccag
    16561 ctgttcaggg caatgtcaac tttctgcgtc cactgattaa gttgctggcg atgctgagta
    16621 atggcttttt ccagcgtctg gataaactgc tgatagttga tccagcggtt gctggtcatc
    16681 ccggcactca tatcgctgtt gaggttattg cgatattcat tctgataatc gatcagcatt
    16741 ttgagctgtt cttccgcctg ctgacacccg cgacgcattt cacccagcag gcgcgcggca
    16801 tcctctacct ctttttctgc cagatctttc agggtcgcca gcgcaccatg ttctgccatc
    16861 taccgttatc tcctgggtta tgacactgtc gggaaaatac gctccagccc ctggagagac
    16921 gcttcccagt ccgcacgttc aaaaatgcct tgttgtaaat agccctccag ctgcggccac
    16981 agggcgatgg ctttatcgag catcggatcg ctgcctttgg catacgcgcc gacgctaacc
    17041 agatcgcggt tacgctgaaa actcgacaac agctgtttga aggtgcgcac tcgcgcgtaa
    17101 tgttgctcac tgatcaacgc cgtcattgcg cggctgatcg acgcttcaat atcgatagcc
    17161 ggatagtgcc cggcttccgc cagtcggcga gacagcacaa tgtgaccgtc gaggatcgcc
    17221 cgtgcggagt ctgcaatggg gtcctgctgg tcatcgcctt cagtaagcac ggtataaaac
    17281 gcggtaatcg agccgccgcc gctaatgcca tttccggcac gctcgaccag tgccggtaat
    17341 ttggcaaaca ccgacggtgg ataacctttg gtggcgggtg gttcgccaat cgccagcgca
    17401 atttcacgct gggccatcgc gtagcgggtg agggagtcca taatcagcaa cacatgctga
    17461 ccacgatcgc gaaaatcttc ggcaatgcgc gtggcatagg cggcaccttg cattcgcaga
    17521 agcggagaga catccgccgg agcggcaatc accacggagc gtgcacgccc ttcagcaccg
    17581 aggatattct caataaaatc ttttacttcg cgcccacgtt caccaatcaa acccacgaca
    17641 atgacatcgg cgcgggtgta acgtgccatc atccccagca gcacactttt accaacgccg
    17701 gacccggcaa acagccccat acgctgccca cgcccaacgg taagcagggc attgattggg
    17761 cgcacgccgg tgtccagcac atgttcaatc ggtgtacgtt gcaacgggtt aaatggcggg
    17821 gtaatcagcg caccggtttc cgtcgtatcg ggggagggca ggccatcgag cggcttaccg
    17881 ctgccgtcca gaacgcgacc taataacgcc ggaccgagcg gcaactgctt gccgctttgc
    17941 agtccttcag ccgaaatgtt tttggcataa acccgcgcgc cgggcaggac accttccact
    18001 tcctccagcg gcattaaaaa cagccgttga ccgttaaagc caacgacttc gctttctact
    18061 tcgtgcgttt cgctgccgtt ctggcgttca atgacacagg ttgcgccgag cggcaattgt
    18121 aatccggtgg cttccagcac cagcccggta gcgcgggtta atcgcccata gcgacgtacc
    18181 gcaggcaact gcgccatttt ggcttcaaag ttatccagcg tggttagcca gcgagtcagg
    18241 cgcgtggtca ttacaccact cctggtgctg ccagacggca gagttcttgc cagcgagtgg
    18301 cgacactggc gtcgagatcg ccttcatcgg cggagacttt acagccgcca ggatggagtg
    18361 tgggatcgcc ccgcaagcgc cagccatgca aacttaaggt tgcgccgagc atatcatcca
    18421 cacgttgcag atcatccggg tgcacgcgca gctgtggttt accgctgaat aacggttctt
    18481 gctgcaacaa ctgttggatc tgtttgatca gtgccgagtt atccactgtt ggcgtctgac
    18541 cgatcacctg acgtgccgcc tccagcgcca tctgcatcag gcgcgaagca atcacactat
    18601 caagcgcatc aagggtagtt tgaaattcgc tgaccagttg ctgcatccgg gcatgaattg
    18661 gcgcttgttg agacttcgcc tctgccagac cttgctccag cccctgggcc agtccttcct
    18721 gatagccctg ctcatgacct tgctggcgac cttcggcaat ccccgcctga taaccttgct
    18781 catgggcctg catttgcagt tgcgccagtt gctgctcaag gctaggttcg gcctcttcaa
    18841 tgatggtttc ttccggctcg accatgggca caaactctgc ctgtggtggc gcgagatcgt
    18901 ccggcgtcca ggttttccac ggcagattat cagacatagg tatcctcgcc gctgccaatt
    18961 accatctcgc cagtttcggc aaggcggcgc acaatcagca gaatcgcttt ctgttcgttt
    19021 tccacctgcg acagacgcac cggaccacgg ttggcgagat cgtcgcgcag aatatcggcg
    19081 gcacgctgcg acatattgcg caggaatttc tcgcgcagtg gctgctcggc ccctttcagc
    19141 gcgatcaaca gcgattcgga atccacttcc tgcaacagac gctgaatgct gcgatcgtcg
    19201 acatccacca gattttcgaa caggaacatc tcgtcgataa ttttttgcgc cagctcgccg
    19261 tcgaattcgc gcacggcggt aataacggct tcttcctgct gagttttcat caggttgata
    19321 atttcggctg ccgttctcac gccgcccatt ttgctgcgct tgagattctg accgtcgagc
    19381 aagccattca gtacttcggt cagttccgcc agcgcggctg gctgcacgcc gccaaaggtg
    19441 gcgatacgca acatcacgtc gtggcgcaga cgttcatcga acaacgccag aatatcggcg
    19501 gcctgcgcgc gcttcagatg caccagaatg gtggcgataa tttgcggatg ctcatcgcga
    19561 atcagatcgg cggcactctg cggctccata aagttgagcg tttcaatacc gctggcggta
    19621 tcgcgagttt cgagaatatc ttccagcagg ctggcggcac gttcctcacc cagagctttg
    19681 accaataccg aacgcagata atcgttggcg ttgatattca gtgcggcaaa ctgttcagct
    19741 tcttgctcaa actccgccag cacataggtt agctgcttgt tggagatctg cgtgacgttc
    19801 gccattgcag cgctcagggt ttgcacttcg cgctgggaga ggtgcttgaa cacctctgcc
    19861 gcccggtctt cgccaatggt catcagcagg atgacgcttt tatcggtgcc tgtcaggtta
    19921 ctcatgatcg ttatttatcc actggcgaat gaccagcgcc accacgcgcg gatcgttatc
    19981 agacatttca cggatacgct ggctcatgac ttctgccccc agacgttggt tagcgcgtcg
    20041 ttgttggagt tgttcgtctt tgctcaggcg gacttccacc gcatcttcca cttcctcacg
    20101 ggcctgcgcc tgttgctgta cagctttcat cgcctcggcg cgacgtgtta actgcgggcg
    20161 taccgctttc cgccacagca gccacgccac cagcagtacc agcaaccagc gaccggcagc
    20221 cagcaactga tcgataaacg cttgctgttg ccagaacggc agttctccgc cgctttcgtc
    20281 actgctattg aacggcgagt taacgacatt gagcgagtca ccgcgttttt cagaaaagcc
    20341 catcgcttcg cgggtcagat cttcaatttg cttcatctgt tcgttgctga gaggcaatgg
    20401 tttaccgtct ggcaaggttt tgtaattcac cactaccgcg actgacagac gttgcacatc
    20461 gcccacgttc attttggtat gacgaatggt gcgatcgact tcgtagttac tggtttcatt
    20521 ccgctgtgtg ctacgcggcc cgctattgct ggtggtgctc gcctgctgct ggcggttatt
    20581 ttgatttgcc ggaggcgtgc tgattggcgc gttattcgca ggtgccggtt gattcgacaa
    20641 cgcccccggt acgccgcccg gataaccgga accgctttgc tcgctctcat taagctggcg
    20701 tgaacgaagc gccgcatgag attcatcacc gttagggcga tactgttctt ccgtttgttc
    20761 tttactggcg aagtccagtt gcgccgtgac ctgggcgtga atattaccgt taccaacaat
    20821 aggcgacagg attgcttcaa tacgccgctg aatacggcct tcgacatcgc tggcatattt
    20881 caactgagcg tcattaagat cgcgcccgct ggtattggac tgggttaaca gatgtccgcc
    20941 ctgatccacc agcgtgacgt ttcccggcgg cagaccagca acggcgctgg aaaccaggtg
    21001 cacaatggcg ctaatttgcc cttcatcgag tgcgcggccg ggtaacagat ttaccgtcac
    21061 cgatgcagaa ggggattttt gttcacggac gaataaagac ggtttcggca ttgccagatg
    21121 tacgcgcgcc ccttttaccg ggccgatagt ttcgatggta cgagaaagct cgccttccag
    21181 cgcccgctga taattcacct gttcgctgaa ctggctgata ccaaactttt cctgatcaag
    21241 cagttcgaaa ccgaccgcgc cgcctttggg caaaccttgt tgtgccaggc gcagacgcag
    21301 ttcgtgaact ttatctgccg gaacttcaat agcgccgctg gcttcgctga agcagtaagg
    21361 aatattcatt tgcgtcagtt ggctgacaat tgcgccacca tcctgatcgg aaagattgct
    21421 gaataatgtg cggtagtcgg gggctttcgc ccacaggatc agtgcgacca tgaccgccac
    21481 tgccgcggaa ccggcaacaa tcaatggaat tttcggattc gcacgcaggc gattaagcca
    21541 ctcaagagat tttgtctggg ctgcagtcgc attcatcgcg cacctcgtgg ctgattatgg
    21601 acggcgttat tggcaaagtg gaacaagact cactcccggg tcagcaaact cgaaaaattg
    21661 acgctgccat tatttgtgct ttcgtttttt tctatccgcc aataaacccg ttttttttgt
    21721 tgctatttag cgcctttgtc ttattgactt actggtaagc tttgctacca gaaattatcc
    21781 gggagatgaa aatgtcagcg atacagggga ttgaaggggt tatcagccag ttacaggcta
    21841 cggcgatgag tgcgcgtgcg caggaatcac tgccacaacc gaccattagt tttgccgggc
    21901 agctgcacgc cgcgctcgat cgcataagcg atacacaaac agccgcccgc acgcaggcag
    21961 aaaaattcac tctcggtgaa cccggcgtgg cgttaaacga tgtgatgacc gatatgcaaa
    22021 aagcctcagt ttctatgcaa atggggattc aggtgcgtaa taagctggtg gcggcttatc
    22081 aggaagtgat gtccatgcag gtgtaggttt tgtaacctgt tgttaattac atccgatctc
    22141 accgcgtggg gcatggatgg ggcaaactca ctcaatttct ggtttaaaat ggatacctga
    22201 tcctgaaagt tttctgccat ccacgatccg taaacgcgat aacatgtttt gtcttcgctt
    22261 tgctcatagg aagagtgaac tcttttgtta gcctggggtt ccttctgatc attattgttc
    22321 ctgcctttat ctcttgccac gccagagccc cctggataca catacaccag gatgaaaacg
    22381 gtgagttgtg ttcaaactgt agtacaattc tctccagtat gaacaggaaa gaatatgcta
    22441 tgaaccctta tatttatctt ggtggcgcaa tacttgcaga ggtcattggt acaaccttaa
    22501 tgaagttttc agaggggttt acacggttat ggccctctgt tggcacaatt atttgctatt
    22561 gtgcatcatt ttggttatta gctcagacac tggcttatat acctacaggg attgcttatg
    22621 ctatctggtc aggagtcggt atcgttctga ttagtttgct ttcctgggga ttttttggcc
    22681 aacgactgga cctgccagcc atcataggca tgatgttgat ttgtgccggt gtgttagtca
    22741 ttaacttgtt gtcacgaagc gcaccgcatt aaaaaataat catccttgag ataacaggaa
    22801 tctgatttgt gcatattatg gctaatgttc ggggcaactt tataattatg attgcatttg
    22861 cgcgatcatt gcgccttttt gcataatgag atcaatgcac atcaaataaa ataacgatag
    22921 gtcacaacaa agaggttttt atgaaaaaac ttatcgtttc aagtgtattg gcattcataa
    22981 cattttctgc gcaggccgca gcatttcagg tcactagtaa tgaaataaaa acaggagagc
    23041 aacttacaac gtctcatgta ttttctggag ttgggtgtga ggggggtaat acatcgccct
    23101 cattaacctg gtctggtgct ccagaaggca ccaaaagctt tgccgtaact gtgtatgatc
    23161 cagatgcgcc tacaggcagc ggatggtggc actggactgt tgctaatatt ccagcaacgg
    23221 taacatattt gcccgctgat gcaggaagac gtgatggtac aaaacttccg actggtgctg
    23281 tccaaggccg aaatgatttt ggctatgcgg gatttggtgg agcatgtcct cccaaaggag
    23341 ataaacccca tcattaccag tttaaaatat gggctctaaa aactgataag attcctgtgg
    23401 attctaattc cagcggagcg ttagttggtt atatgcttaa tgccaataaa attgcaaccg
    23461 ctgagataat acccgtttat gaggtaaagt aggataagag tatgctggca aaagatagga
    23521 ctaacctaaa gattgaagaa atacggatgc ataaacatca tgagattcac agggttaagc
    23581 ctcttatgcc agcttcatgt cgtatccgtc agggaaaaaa agttatcaat tgggagacgc
    23641 atagtctaac tgttgataat aatcagataa tattatttcc ctgtggatat gaattttata
    23701 ttgcgaatta cccagaagca ggtctttatc ttgcagaaat gctttattat cccattgatc
    23761 taattgaaaa gttccaaaat ctttatgcga taaccgatca aattcgtaac acgacaggtt
    23821 tctgcttacc tcagaattcc gagttaatat attgttggga gcaactaaaa acatctattt
    23881 cccgaggctt ctcaacgcaa attcaggaac acttagcaat gggcgttcta ctttcattag
    23941 gagcacatca tgttaattgt ttacttttat cagatagtaa acaatcatta ataagtcgtt
    24001 gttataacct aatgctatcc gaacctggaa caaaatggac agcaaacaag gtagcgagat
    24061 atctctacat ttctgtttcc acattgcatc gccgtctggc aagcgaggga gtaagttttc
    24121 aaagtatatt ggacgacgtg aggttaaata atgcgttgtc tgctatacaa acgacagtaa
    24181 aacccatcag cgagattgcc agggaaaatg gttacaagtg tccttctcgt tttactgaaa
    24241 ggttccataa tcgttttaag ataacaccaa gagagctaag aaaagcgtcc agagagtaaa
    24301 agtattttat gaaggagcaa cagcatcgat ttttattttg gaaaataaat acggcatact
    24361 caacctattg gagcctgaac aatccggtga cttctgcgct aatcggggac gtttataagc
    24421 acatataatt gatactatct tcctccatca caatttcaga aatgcacgtg tgattttttt
    24481 gcatccggcg tttggactat gtggagttca agagtgggaa agttacaaga ggttgagggg
    24541 ataattaaag ggatgcttat gacattagat atccggatag gtacggatat cgttgttatt
    24601 aaatctagtt cgttattatt aaatctagtt tatacgttaa gatgcagcta cagcagaaat
    24661 tgccactgtt aattttttca tcgtgagccc ttttatttga actattattt aaaaatgatg
    24721 tcactgcact acaaatattc atccaatcaa tgagattatt tcaagatgta agttttagtt
    24781 tctcatttaa attgtgaagt ag
//
LOCUS       DAJVYM010000049        24678 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVYM010000049 DAJVYM010000000
VERSION     DAJVYM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.049"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="OQS60_001858"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="OQS60_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS2387631.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="OQS60_001859"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="OQS60_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="OQS60_001860"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="OQS60_001860"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS2387632.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="OQS60_001861"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="OQS60_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS2387633.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="OQS60_001862"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="OQS60_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS2387634.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="OQS60_001863"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="OQS60_001863"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS2387635.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="OQS60_001864"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="OQS60_001864"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS2387636.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="OQS60_001865"
     CDS             8330..10048
                     /locus_tag="OQS60_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS2387637.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="OQS60_001866"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="OQS60_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS2387638.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="OQS60_001867"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="OQS60_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS2387639.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="OQS60_001868"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="OQS60_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS2387640.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /locus_tag="OQS60_001869"
     CDS             complement(12751..13440)
                     /locus_tag="OQS60_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS2387641.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="OQS60_001870"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="OQS60_001870"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS2387642.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="OQS60_001871"
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="OQS60_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS2387643.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="OQS60_001872"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="OQS60_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS2387644.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="OQS60_001873"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="OQS60_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS2387645.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="OQS60_001874"
     CDS             complement(20443..20718)
                     /locus_tag="OQS60_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387646.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="OQS60_001875"
     CDS             complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="OQS60_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS2387647.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23165..23482
                     /locus_tag="OQS60_001876"
     CDS             23165..23482
                     /locus_tag="OQS60_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS2387648.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23551..23880
                     /gene="yjdP"
                     /locus_tag="OQS60_001877"
     CDS             23551..23880
                     /gene="yjdP"
                     /locus_tag="OQS60_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS2387649.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(24027..>24678)
                     /gene="phnP"
                     /locus_tag="OQS60_001878"
     CDS             complement(24027..>24678)
                     /gene="phnP"
                     /locus_tag="OQS60_001878"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS2387650.1"
                     /translation="RQPCSGVVKFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMD
                     HVQGLFPLRWGVGDPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTP
                     LPLNHSKLTFGYLLETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADA
                     PRNHCDLNTVLALNQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
BASE COUNT         5596 a         6466 c         6726 g         5890 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc taggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggt
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    20821 ttcagtcacc gcattcataa cttatatatt ttatttatta attggctgta tatattttta
    20881 aaataaatgc ttttgggggc ttcagcgccc ccaaaagcga gtgacattaa tttggggtta
    20941 cgatattcac ccacaaatcg aaattatcca gacaggcgat aatttcttcc agtttcgcgc
    21001 cattgccaat aattttggct ttcttcgctt ttaccagatc cgccattttg gcttgcccgg
    21061 tcagcacggc gtgcagatct tcacggctga tgtagaaaga ggcgtcagct tgggattgca
    21121 gtgttttacg gtagttaagc acgctatcgt tcagcgtcag gttgaggtta tcgccattgc
    21181 tcatattgaa gttcaggctg atatttttac ccgcggcttt cgcgctatcg aggcgaacgg
    21241 acataaagtc gaacagcatt tcgaccgaca tcccgcgaat ggtgtccggg gaaccgatgg
    21301 tgccgtggct gaacttatgc accccttcgc gcagctcttt cgcgccggtc aggtagaaac
    21361 cgcgccaggt ggcggactcg gcctgatagc ccagctgttc aaagttattc gcttgcagat
    21421 tcttcgcgac ctggtcaccc gggttggcgg caatcacctg tttcagcagt tctgccgacc
    21481 agcggtaatc accttgcttg ttcgcttctt gcgccaggtt gatgacacgg gcagaaccgc
    21541 ccagcgcctg cacgtaacgt ttacccatct ccacctgacc atacggatgc aggttagccg
    21601 ggttaccgtc gtaatagcca agatagaagt tatacaccgc gcgggcgttg tggctgacag
    21661 aaccgtaata gccacggctg gcccagttat tggcaagtgc aggcggcagt ttaatcatgt
    21721 cgccgatttc attcatggtg tagccctggt tcgccaggtg cagggtctgg tcgtgaatgt
    21781 acttgatagt atcgcgatat ttaccaatat aatcattgat atgcttattg ccccagaccg
    21841 gccaggtgtg cggcataaac aggacttccg cgtcgttacc ccacatatcc agcgtttcgt
    21901 tcagatactc ggtccacttg ctggtgtcgc gggttttcgc gccgcgcaga gtgtagaagt
    21961 tgtgcagggt atgcgtggcg ttctcggcgg tacacagcgc tttcagggcc ggaatataga
    22021 agtgcatttc ggctggtgct tcgctacctg gggtcatcag gaagtcaaac tccaggccgt
    22081 cgataatcat cttctcaccg gttttggtga tggttttgtt cggtgcgatg atgctcgggt
    22141 agcccgatgc cagcgttacg ccaagaccat tgccgatgtt accctgaggg ttatgcgcca
    22201 gcagcaggcc gtaggagtaa agtgcacggc ggctcataat attacccgcc agtacgtttt
    22261 cgctgatggc ttcgtccata aagccagcag gggcgataac ctgcacttta ccggatttca
    22321 catcagcttc agagataatg cctttcacgc cgccatagtg gtcggtatgg ctgtgggtat
    22381 aaataaccgc aacaatcggt ttttgcgggc gattttggaa gtaaaggtca agggcggctt
    22441 ttgcgctcgg tggcgtcacc agcgggtcga tgacaataat cccggtatca ccttcgataa
    22501 aggtgatgtt ggagatatcc tgaccgcgaa cctggtacat tctgtcggtg actttaaaca
    22561 ggccggaaat accgtttaac tgcgactgac gccacaggct agggttaacg gtttcgggcg
    22621 cgggcgcatt gatatcaaat ttataatctt ccccgcgata atatggtttg ccattagcat
    22681 cgcgcagaat accttcatcc agcagcggag cgataaatcc acgctgagcg tcgtcaaaat
    22741 cctgacgatc gctaaacggt aatgatttgg cgtaattctg attaacctgt tgggtatatt
    22801 gagtggcggc tttcgcttcc tctttcgcgt tagcggtatt taccatcatg ccgctggcgg
    22861 cagttaacgc aataacaatt ctgctcaaac ggaataaccg agagttattc atgtaaatct
    22921 ccatttatca tttatgtcgt aaatatgtaa tccatgcaaa atacgtcatt taaagctggg
    22981 gttatcataa ttgggaattt gcgactttaa taagtggaag tgtgagctga acgatccatt
    23041 ttattaggca tttgtgctga agagggaaaa ttacgcgaat ttattatctc tgtaaataac
    23101 atgaggtgtt ttatatattt attatatata ttgtacttcg gcaaatactg agcacaattt
    23161 atcaatgcaa ccggtgtttc tttttaaaaa acgtaccgga gcaatgggcg gtcatgtcgc
    23221 tggcaccgca ggattctgcg ttgctgtgcc agccttttat tcatggtcaa tgagtgaggt
    23281 gattgataaa gacaccaggt cgtcagagcg cactgattat ccgcattatg ccagtgaagt
    23341 tgcactggct tttggttcac gagtggttgg cctcgaactg gcaaaaatga ttgtggatgc
    23401 gtggctgggc gcacagtacg aaggcggtcg tcatcaacaa cgcgtggagg cgattacggc
    23461 aatagagcag cggagaaatt gagattcatc cactacttgc atggatgagt aatgactaat
    23521 gtggatagag tttctttttg aggttggcta atgaaacgct ttccgctatt tcttttattc
    23581 accctgctca cgctgtccac cgttccggcc caggctgata ttatcgacga tactattggt
    23641 aatattcagc aggcgattaa tgacgcttat aaccctgacc gtgggcgaga ctatgaagat
    23701 tcgcgcgacg acggctggca gcgtgaagtg agcgacgatc ggcgcagaca atatgacgac
    23761 cgccgccgcc agtttgaaga tcgccgcagg cagctggacg atcgccagcg ccagcttgat
    23821 caggagcgtc ggcaactgga ggatgaagag cggagaatgg aagatgagta tgggcgatga
    23881 ggttgggtat ggagcaggca tcgcccctca ccccaatggg gagagggaga aaacgagcgc
    23941 aatattcaat attgagcaca atcggtcccc tcgcccctct ggggagaggg ttagggtgag
    24001 ggggccagcg cgcacattcc ctctcatcac gccaccccaa tctccatccc atcaaacccc
    24061 acctcaaacc ctgacggtag tgcgttttcc atcagccacg catcaaactg gtggctgata
    24121 tgggtcagaa tcacccgtgg cgagcggata acctgattca gcgcaagcac ggtatttaaa
    24181 tcacagtgat tacgcggtgc atccgcgcgc ggcgggtgac tgcaatccat caccattacc
    24241 tgcggctgat tattgcgtaa aaatttcagc gttttttccg gcaagccagc ggtgtcagac
    24301 agccacgcca cccggctgtg cgccgtttcc agtaaatagc cgaaagtgag ttttgagtgg
    24361 ttaagcggca gcggcgtgac ctgcaacccc tgcaaatcaa acaccacaaa cggttccacc
    24421 gtgtggctga aatcaagcag cccgggatgt ttaaacagat cgtcgcagcc ctgttcatcc
    24481 ggcgggccgt agatcgggat cggatcgccc acgccccagc gcagcggaaa cagcccctgg
    24541 acgtgatcca tatgataatg cgtcagcaaa aactgctgga acgatccggg agaccagcga
    24601 tcggtgagat cgtgcagccc ggcgtcgatc agggtgatcg cctcgttaaa cttcactacg
    24661 ccgctgcacg gttggcgg
//
LOCUS       DAJVYM010000050        24458 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVYM010000050 DAJVYM010000000
VERSION     DAJVYM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.050"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..196)
                     /locus_tag="OQS60_001879"
     CDS             complement(<1..196)
                     /locus_tag="OQS60_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCS2387651.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSD"
     gene            585..1130
                     /locus_tag="OQS60_001880"
     CDS             585..1130
                     /locus_tag="OQS60_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCS2387652.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1105..3030
                     /locus_tag="OQS60_001881"
     CDS             1105..3030
                     /locus_tag="OQS60_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS2387653.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3027..3233
                     /locus_tag="OQS60_001882"
     CDS             3027..3233
                     /locus_tag="OQS60_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCS2387654.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3230..4831
                     /locus_tag="OQS60_001883"
     CDS             3230..4831
                     /locus_tag="OQS60_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS2387655.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4812..6131
                     /locus_tag="OQS60_001884"
     CDS             4812..6131
                     /locus_tag="OQS60_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS2387656.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6141..6473
                     /locus_tag="OQS60_001885"
     CDS             6141..6473
                     /locus_tag="OQS60_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS2387657.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6529..7554
                     /locus_tag="OQS60_001886"
     CDS             6529..7554
                     /locus_tag="OQS60_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS2387658.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7596..7994
                     /locus_tag="OQS60_001887"
     CDS             7596..7994
                     /locus_tag="OQS60_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS2387659.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8006..8359
                     /locus_tag="OQS60_001888"
     CDS             8006..8359
                     /locus_tag="OQS60_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCS2387660.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8371..8949
                     /locus_tag="OQS60_001889"
     CDS             8371..8949
                     /locus_tag="OQS60_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2387661.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8946..9341
                     /locus_tag="OQS60_001890"
     CDS             8946..9341
                     /locus_tag="OQS60_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2387662.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9349..10089
                     /locus_tag="OQS60_001891"
     CDS             9349..10089
                     /locus_tag="OQS60_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2387663.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10105..10527
                     /locus_tag="OQS60_001892"
     CDS             10105..10527
                     /locus_tag="OQS60_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS2387664.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10509..10943
                     /locus_tag="OQS60_001893"
     CDS             10509..10943
                     /locus_tag="OQS60_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS2387665.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10936..13497
                     /locus_tag="OQS60_001894"
     CDS             10936..13497
                     /locus_tag="OQS60_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS2387666.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13494..13823
                     /locus_tag="OQS60_001895"
     CDS             13494..13823
                     /locus_tag="OQS60_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2387667.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13823..14521
                     /locus_tag="OQS60_001896"
     CDS             13823..14521
                     /locus_tag="OQS60_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS2387668.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14527..15270
                     /locus_tag="OQS60_001897"
     CDS             14527..15270
                     /locus_tag="OQS60_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2387669.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15207..15839
                     /locus_tag="OQS60_001898"
     CDS             15207..15839
                     /locus_tag="OQS60_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS2387670.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15900..19397
                     /locus_tag="OQS60_001899"
     CDS             15900..19397
                     /locus_tag="OQS60_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS2387671.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19468..19984
                     /locus_tag="OQS60_001900"
                     /pseudo
     CDS             19468..19984
                     /locus_tag="OQS60_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20049..21453
                     /locus_tag="OQS60_001901"
                     /pseudo
     CDS             20049..21453
                     /locus_tag="OQS60_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            21402..21554
                     /locus_tag="OQS60_001902"
                     /pseudo
     CDS             21402..21554
                     /locus_tag="OQS60_001902"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            21531..21710
                     /locus_tag="OQS60_001903"
                     /pseudo
     CDS             21531..21710
                     /locus_tag="OQS60_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22425..23132
                     /locus_tag="OQS60_001904"
                     /pseudo
     CDS             22425..23132
                     /locus_tag="OQS60_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23132..23713
                     /locus_tag="OQS60_001905"
     CDS             23132..23713
                     /locus_tag="OQS60_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2387672.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5850 a         6075 c         7419 g         5114 t
ORIGIN      
        1 tgtcactctt ttctttgttt gcttcaggcg aaactctttt ttctgagcac agtctccggc
       61 ggcaggcttc aatgacccag gctgagaaat tcccggaccc tttttgaaca agagcgatgt
      121 taatttgttc aatcatttgg ttaggaaagc ggatgttgcg ggttgttgtt ctgcgggttc
      181 tgttcttcgt tgacatgagg ttgccctgta ttcagtgtcg ctgatttgta ttgtctgaag
      241 ttgtttttac gttaagttga tgcagatcaa ttaatacgat acctgcgtca taattgatta
      301 tttgacgtgg tttgatggcg tagatgcacg ttgtgacatg tagatgataa ttattatcat
      361 tttgcgggtc ctttccggcg atccgacagg ttacggggcg gaaggcgcgc gggttttcgc
      421 tatttatgac aattttccgg tttaaggcgt ttccgttctt cttcgccgta acctaatgtt
      481 tttatttaaa acaccccctg aaaagaaagg aaacgacagg tgctgaaaac gggctttttg
      541 gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcaacaaaa
      601 agcagctggc tgacattttc ggtgcgagta tccgtaccat tcagaactgg caggaacagg
      661 gaatgcccgt tctgcgaggc ggtggcaagg gtaatgaggt gctttatgac tctgccgccg
      721 ttataagatg gtatgccgaa agggatgctg aaattgagaa cgaaaagctg cgccgggaag
      781 ttgaagaact gcggcaggcc agcgagacag atctccagcc agggactatt gagtacgaac
      841 gccatcgact tacgcgtgcg caggccgacg cacaggagct gaaaaatgcc agagactccg
      901 ctgaagtggt ggaaaccgca ttctgtactt tcgtgctgtc gcggatcgca ggtgaaattg
      961 ccagtattct cgacgggatc cccctgtcgg tgcagcggcg ttttccggaa ctggaaaacc
     1021 gacatgttga tttcctgaaa cgggatatca tcaaagccat gaacaaagca gccgcgctgg
     1081 atgaactgat accggggttg ctgagtgaat atatcgaaca gtcaggttaa caggctgcgg
     1141 cattttgtcc gcgccgggct tcgctcactg ttcaggccgg agccacagac cgccgttgaa
     1201 tgggcggatg ctaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa
     1261 acactgccct ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgtgaggtg
     1321 aatgtggtga agtctgcccg tgtcggttat tccaaaatgc tgctgggtgt ttatgcctac
     1381 tttatagagc ataagcagcg caacacactt atctggttgc cgacggatgg tgatgccgag
     1441 aactttatga aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg
     1501 gctccgtggt atggcaaaaa gcaccgggat aacacgctca ctatgaagcg tttttccaat
     1561 ggtcgtggct tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg
     1621 gatgtggcgg gttatgatga acttgctgcc tttgatgagg atattgaaca ggaaggctct
     1681 ccgacgttcc ttggcgacaa acgtattgaa ggctcggtct ggccaaagtc catccgtggc
     1741 tccacgccca aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat
     1801 tttatgcgtt ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc
     1861 gacaaagaga cgccgtttgg cctcaaatgg acgccggatg acccctccag cgtgttttat
     1921 ctctgcgagc ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt
     1981 tatatctgcg aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc
     2041 ggtgaagaga ttgagccacc tgacagtgtg acctttcaca tctggacagc gtacagcccg
     2101 ttcaccacct gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa
     2161 cgtaaaacct tcgtaaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt
     2221 ccggatgctg aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt
     2281 gtggcttacc tgaccgccgg tatcgactcc cagcgggatc gctacgaaat gcgcgtatgg
     2341 ggatgggggc cgggtgagga aagctggctg attgatcggc agattattat gggccgccac
     2401 gacgatgaac agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg
     2461 aatggtgcag aaatgtcgat atcccgtatc tgctgggata ttggcgggat tgacccgacc
     2521 attgtgtatg aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca
     2581 tccgtctacg gtaagccggt ggccagcatg cctcgtaagc gaaacaaaaa cggggtttac
     2641 cttaccgaaa tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg
     2701 ccggaagggg atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt
     2761 gatctgaccg aagcgcagca gctgactgct gaagagcagg tcgaaaaatg ggtggatggc
     2821 aggaaaaaaa tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt
     2881 tatgcgctgg cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg
     2941 ctggcgagcc tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac
     3001 gcccgtgcct tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg
     3061 cggcactgca tgacctgatg acaggtaaac gggtggcaac ggtacagaaa gacggacggc
     3121 gagtggagtt tacggccact tccgtgtctg acctgaaaaa atacattgcg gagctggagg
     3181 tgcagaccgg catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc
     3241 caccattccc acccttctgg gaccggacgg catgacatcg ctgcgtgaat atgccggtta
     3301 tcacggcggt ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag
     3361 tgtggatgca gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt
     3421 acgcaataac ggctatgctg ccaacgccat ccagctgcat caggatcata tcgtcgggtc
     3481 ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagacgc
     3541 ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg
     3601 ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat
     3661 gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct
     3721 tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg
     3781 cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata
     3841 ttacgtcagc gaggacggct atcctggctg gatgccgcag aaatggacat ggataccccg
     3901 tgagttaccc ggcgggcgcg cctcgttcat tcacgttttt gaacccgtgg aggacgggca
     3961 gacccgcggt gcaaatgtgt tttacagcgt aatggagcag atgaagatgc tcgacacgct
     4021 gcagaacacg cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag
     4081 tgagctggat acgcagtcag cgatggattt tattctgggc gcgaacagta aggagcagcg
     4141 ggaaaggctg acgggctgga ttggtgaaat tgccgcgtat tacgccgcag caccggtccg
     4201 tctgggaggc gcaaaagtgc cgcacctgat gccgggggac tcactgaacc tgcagacggc
     4261 tcaggacacg gataacggct actccgtgtt tgagcagtca ctgttgcggt atatcgctgc
     4321 cgggctgggt gtctcgtatg agcaactttc ccggaattac gcccagatga gctactccac
     4381 ggcacgggcc agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc
     4441 atcccgtcag gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt
     4501 ggtgacgtta ccttcaaaag cgcgcttcag ctttcaggaa gcccgcagtg cctgggggaa
     4561 ctgcgactgg ataggctccg gtcgtatggc catcgatggt ctgaaagaag ttcaggaagc
     4621 ggtgatgctg atagaagccg gactgagcac ctacgagaaa gagtgcgcga aacgcggtga
     4681 cgactatcag gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagctgg
     4741 tcttaaaccg cccgcctggg cggctgcggc atttgaatcc gggctgcgac aatcaacaga
     4801 ggaggagaag agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta
     4861 atgagccgct gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc
     4921 agcttgggat cagctgcctg acggatgcag tatccggcga cagcctgact gccggagagg
     4981 cacccgcggc gctggcgtta tccggtgatg atgacggacc acgacaggcc cgcagttatc
     5041 aggtaatgaa cggcatcgcc gtgctgccgg tgtccggcac gctggtcagc cggacgcggg
     5101 cgctgcagcc gtactcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg
     5161 ctgccagcga cccgatggtg gacggcattc tgctcgatat ggacacaccg ggcggaatgg
     5221 tggcgggagc atttgactgc gctgacatca tcgcccgtgt gcgtgacata aagccggtat
     5281 gggcgctggc caatgacatg aactgcagcg caggtcagct gcttgccagt gccgcctccc
     5341 ggcgtctggt cacgcagacc gcccggacag gctccatcgg cgtcatgatg gctcacagta
     5401 attacggcgc tgcactggag aaacagggcg tggaaatcac gctgatttac agcggcagcc
     5461 ataaggtgga tggcaacccc tacagccatc taccggatga tgtccgggaa acattgcagt
     5521 cccggatgga tgcaacccgc cggatgtttg cacagaaggt gtcggcatat accggcctgt
     5581 ccgtgcaggc tgtgctggat accgaggctg cagtgtacag cggtcaggag gccattgatg
     5641 ccggactggc tgatgaactt gttaacagta ccgatgcgat caccgtcatg cgtgatgcac
     5701 tggatgcacg taaatcccgt ctctcaggag ggcgaatgac caaagagact caatcaacaa
     5761 ctgtttcagc cactgcttcg caggctgacg ttactggcgt ggtgcaagcg acggagggcg
     5821 agaacgccag cgcggcgcag ccggacgtga acgcgcagat caccgcagcg gttgcggcag
     5881 aaaacagtcg cattatgggg atcctcaact gtgaggaggc tcacggacgc gaagaacagg
     5941 cacgcgtgct ggccgaaacc cccggtatga ccgtggaaac ggcccgccgt attctggccg
     6001 cagcaccaca gagtgcacag acgcgcagtg acactgcgct ggatcgtctg atgcaggggg
     6061 caccggcacc gctggctgca ggtaacccgg catctgatgc cgttaacgat ttgctgaaca
     6121 caccagtgta agggatgttt atgacgagca aagaaacctt tacccattac cagccgctgg
     6181 gcaatagtga cccggctcat accgcaaccg cgcccggcgg attgagtgcg aaagcgcctg
     6241 caatgacccc gctgatgctg gacacctcca cccgtaagct ggttgcgtgg gatggcacca
     6301 ccgacggtgc tgccgttggc attcttgcgg ttgctgctga ccagaccagc accacactga
     6361 cgttctacaa gtccggcacg ttccgttatg aggatgtgct ctggccggag gctgccagcg
     6421 acgagacgaa aaaacggacc gtgtttgccg gaacggcaat cagcatcgtt taactttacc
     6481 cttcatcact aaaggccgcc tgtgtggctt tttttacggg atttttttat gtcgatgtac
     6541 acaaccgccc agctgctggc ggcaaatgag cagaaattta agtttgatcc gctgtttctg
     6601 cgtctctttt tccgtgagag ctatcccttc actacggaga aagtctatct ctcacaaatt
     6661 ccgggactgg taaacatggc gctgtacgtt tcgccgattg tttccggtga ggttatccgt
     6721 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaggtg
     6781 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
     6841 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgct
     6901 caggtcgaag agatgcaggc agtttctgcc gtgctcaagg gcaaatacac catgaccggt
     6961 gaagccttcg atccggttga ggtggatatg ggccgcagtg cggcgaacaa catcacacag
     7021 tccggcgtca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
     7081 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga cccgaaaggc
     7141 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
     7201 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtccta taagggaatg
     7261 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
     7321 aacttcctgc cggacaacac gatggtgctg gggaacactc aggctcgcgg tctgcgcacc
     7381 tatggctgca ttcaggatgc ggacgcacag cgcgaaggca ttaacgcttc tgcccgttac
     7441 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
     7501 ccgctgatgc tgctggctga tcctgatgag ttcgtgtccg ttcaactggc gtaatcatgg
     7561 cccttcgggg ccattttctc tctgtggagg agttcatgac gaaagatgaa ctgattgccc
     7621 gtcttcagga gctgggtgag caattgaacc gcgatgtcag tttgacggga acgaaagaag
     7681 aactggcgct tcgtgtggca gagctggaag aagagcttga tgacacggat gaaactgccg
     7741 gtcaggacac ccctctcagc cgggaaaatg tgctgaccgg gcatgaaaat gaggtggtat
     7801 cagcgcagcc ggataccgtg attcaggata cggctgaact ggtcacggtc gtggcactgg
     7861 tgacgctgca tactgatgca cttcacgcca cgcgggataa acctgtggca tttgtgctgc
     7921 cgggaacggc gtttcgtgtc tctgccggtg tggcagccga aatgacagag cgcggcctgg
     7981 ccagaatgca ataacgggag gcgctgtggc tgatttcgat aacctgttcg atgctgccat
     8041 tgcccgcgcc gatgaaacga tacgcgggta catgggaacg tcagccacca tgacatccgg
     8101 tgagcagtca ggtgcggtga tacgtggtgt ttttgatgac cctgaaaata tcagctatgc
     8161 cggacagggc gtgcgcgttg aaggctccag cccgtccctg tttgtccgga ctgatgaggt
     8221 gcggcagctg cggcgtggag acacgctgac catcggtgag gaaaatttct gggtagatcg
     8281 ggtttcgccg gatgatggcg gaagttgtca tctctggctt ggacggggcg taccgcctgc
     8341 cgttaaccgt cgccgctgaa agggggatgt atggccataa aaggtcttga gcaggccgtt
     8401 gaaaacctca gccgtatcag caaaacggcg gtgcccggtg ccgccgcaat ggccattaac
     8461 cgcgttgctt catccgcgat atcgcagtct gcgtcacagg ttgcccgtga gacaaaggta
     8521 cgccggaaac tggtaaagga aagggccagg ctgaaaaggg ccacggtcaa aaatccgcag
     8581 gccagaatca aagttaaccg gggggatttg cccgtaatca agctgggtaa tgcgcgggtt
     8641 gtcctgtccc gccgcaggcg tcataaaaag gggcagcgtt catccctgaa aggtggcggc
     8701 agcgtgcttg tggtgggtaa ccgtcgtatt cccggcgcgt ttattcagca actgaaaaac
     8761 ggccggtggc atgttatgca gcgtgtggcc gggaaaaacc gttaccccat tgatgtggtg
     8821 aaaatcccga tggcggtgcc gctgaccacg gcgtttaaac agaatattga acggatacgg
     8881 cgtgaacgtc ttccgaaaga gctgggctat gcgctgcagc atcaactgag aatggtaata
     8941 aagcgatgaa acatactgaa ctccgtgcag ccgtactgga tgcactggag aagcatgaca
     9001 ccggggcgac gctttttgat ggtcgccccg ctgtttttga tgaggaagat tttccggcaa
     9061 ttgccgttta tctcaccggc gctgaataca cgggcgaaga gctggacagc gatacctggc
     9121 aggcggagct gcatattgaa gttttcctgc ctgctcaggt gccggattca gagctggatt
     9181 cgtggatgga gtcccggatt tatcctgtga tgagcgatat cccggcactg tcagatttga
     9241 tcaccagtat ggtggccagc ggctatgact accggcgcga cgatgatgcg ggcctgtgga
     9301 gttcagccga tctgacttat gtcattatct atgaaatgtg aggacgctat gcctgtacca
     9361 aatcctacaa tgccggtgaa aggtgccggg accaccctgt gggtttataa ggggagcggt
     9421 gacccttatg cgaacccgct ttcagacgtt gactggtcgc gtctggcaaa agttaaagac
     9481 ctgacgcccg gcgaactgac cgctgagtcc tatgacgaca gctatctcga tgatgaagat
     9541 gcagactgga ctgcgaccgg gcaggggcag aaatctgccg gagataccag cttcacgctg
     9601 gcgtggatgc ccggagagca ggggcagcag gcgctgctgg cgtggtttaa tgaaggtgat
     9661 acccgtgcct ataaaatccg cttcccgaac ggcacggtcg atgtgttccg tggctgggtc
     9721 agcagtatcg gtaaggcggt gacggcgaag gaggtgatta cccgcacggt gaaggtcacc
     9781 aatgtgggac gtccgtcgat ggcagaagat cgcagcacgg tgacggcgac gaccggcatg
     9841 acggtgacac ccgccagtgc ttccgtagtg aaagggcaga gcaccacgct gaccgtggca
     9901 ttccagccgg atggcgcaac cgacaagagc ttccgtgcgg tgtctgcgga taaaacaaaa
     9961 gccaccgtgt cggtcagtgg tatgaccatc accgtgaaag gtgttgctgc aggcaaggtc
    10021 aacattccgg tcgtatctgg taatggtgag tttgctgcgg ttgcagaaat caacgtcacc
    10081 gccagttaat ccggagagtc agcgatgttc ctgaaaaccg aatcatttga acataacggt
    10141 gtgaccgtca cgctttctga actgtcagcc ctgcagcgta ttgagcatct cgacctgatg
    10201 aaacggcagg cagaacaggc ggagtcagac agcaaccgga agtttactgt ggaagacgcc
    10261 atcagaaccg gcgcttttgt ggttgcgatg tccctgtggc ataaccatcc gaagaagacg
    10321 cagatgccgt ccatgaatga agccgttaaa cagattgagc aggaagtgct taccacctgg
    10381 cccacagagg caatttctca tgctgaaaac gtggtgtacc ggctgtccgg tatgtatgag
    10441 tttgtggtga atgatgctcc tgaacaggca gaggacgccg ggcctgcaga gcctgtttct
    10501 gcgggaaagt gttcgacggt gagctgagtt ttgccctgaa actggcgcgt gagatggggc
    10561 gacccgactg gcgcgccatg cttgccggga tgtcatccac ggagtatgcc gactggcacc
    10621 gcttttacag tacccattat tttcatgatg ttctgctgga tatgcacttt tccgggctga
    10681 cgtacaccgt gctcagcctg tttttcagcg atccggagat gcatccgctg gatttcagtc
    10741 tgctgaaccg gcgcgaggct gacgaagagc ctgaagatga tgtgctgatg cagaaagcgg
    10801 cagggcttgc cggaggtgtc cgctttggcc cggacgggaa tgaagttatc cccgcttccc
    10861 cggatgtggc ggacatgacg gaggatgacg taatgctgat gacagtatca gaagggatcg
    10921 caggaggagt ccggtatggc tgaaccggta ggcgatctgg tcgttgattt gagtctggat
    10981 gcggccagat ttgacgagca gatggccaga gtcaggcgtc atttttccgg tacggaaagt
    11041 gatgcgaaaa aaacagcggc agtcgttgaa cagtcgctga gccgacaggc gctggctgca
    11101 cagaaagcgg ggatttccgt cgggcagtat aaagccgcca tgcgtatgct gcctgcacag
    11161 ttcaccgacg tggccacgca gcttgcaggg gggcagaatc cctggctgat cctgctgcaa
    11221 cagggtggtc aggttaagga ctccttcggc gggatgatcc ccatgttcag ggggcttgcc
    11281 ggcgcgatca ccctgccgat ggtgggggcc acctcgctgg cggtggcgac cggtgcgctg
    11341 gcgtatgcct ggtatcaggg caactcaacc ctgtccgatt tcaacaaaac gctggtcctt
    11401 tccggtaatc aggcgggact gacggcagat cgtatgctgg tcctgtccag agccgggcag
    11461 gcggcagggc tgacgtttaa ccagaccagc gagtcactca gcgcactggt taaggcgggg
    11521 gtaagcggtg aggctcagat tgcgtccatc agccagagtg tggcgcgttt ctcctatgca
    11581 tccggcgtgg aggtggacaa ggtcgctgaa gccttcggga agctgaccac agacccgacg
    11641 tcggggctga cggcgatggc acgccagttc cataacgtga cggcggagca gattgcgtat
    11701 gttgctcagt tgcagcgttc cggcgatgaa gccggggcat tgcaggcggc gaacgaggcc
    11761 gcaacgaaag ggtttgatga ccagacccgc cgcctgaaag agaacatggg cacgctggag
    11821 acctgggcag acaggactgc acgtgcattc aaatccatgt gggatgcggt gctggatatt
    11881 ggtcgtcctg ataccgcgca ggagatgctg attaaggcag aggctgcgtt taagaaagca
    11941 gacgacatct ggaatctgcg caaggatgat tatttcgtta acgatgaagc acgggcgcgt
    12001 tactgggatg atcgtgaaaa aaaacgcctg gagcgggatg cagcccaaaa gagggtagat
    12061 caacagcgtc aacaggacaa aaatgcgcag cagcagagcg ataccgaagc gtcacggctg
    12121 aaatataccg aagaggcgca gaaggcttac gaacggctgc agacgccgct ggagaaatat
    12181 accgcccgtc aggaagaact gaacaaggca ctgaaagacg gaaaaatcct gcaggcagat
    12241 tacaacacgc tgatggcggc ggcgaaaaag gattatgaag cgacgctgaa aaagccgaaa
    12301 cagtccggcg tgaaggtgta tgcgggcgat cgtcaggaag acagtgctca tgcggccctg
    12361 ctgacgcttc aggcagaact ccggacgctg gagaagcatg ccggagcgaa tgagaaaatc
    12421 agccagcagc gccgggattt gtggaaggca gaaagtcagt tcgcggtact ggaggaggcg
    12481 gcacaacgtc gccagctgtc cgcacaggag aaatccctgc tggcgcataa agacgagacg
    12541 ctggagtaca aacgccagct ggctgcactt ggtgacaagg ttacgtatca ggagcacctg
    12601 aatgcgctgg cgcagcaggc ggataaattc gcacagcagc aacgggcaaa acgggcagcc
    12661 attgatgcga aaaaccgggg gctgactgac cggcaggcag cgcgggaagc cacggaacag
    12721 cgcctgaagg aacagtatgg cgataatcct ctggcgctga ataacgtcat gtcagagcag
    12781 aaaaagacct gggcggctga agaccagctt cgcgggagct ggatggcagg cctgaagtcc
    12841 ggctggagtg agtgggaaga gagcgccacg gacagtatgt cacaggttaa aagtgcagcc
    12901 acgcagacct ttgatggtat tgcacagaat atggcggcga tgctgaccgg cagtgaacag
    12961 aactggcgca gcttcacccg ctccgtgctg tccatgatga cagaaattct gcttaagcag
    13021 gcaatggtgg ggattgtcgg gagtatcggc agcgccattg gcggggctgt tggtggcggc
    13081 gcatccgcgt caggtggtac agccattcag gccgctgcgg tgaaattcca ttttgcaacc
    13141 gggggattta cgggaaccgg cggcaaatat gagccagcgg ggattgttca ccgtggtgaa
    13201 tttgtcttca cgaaggaggc aaccagccgg attggcgtgg ggaatctcta ccggctgatg
    13261 cgcggctatg cggaaggtgg ttatgtgggc ggtgccggaa gtccggcgca gatgcggcgg
    13321 gctgaaggca ttaattttaa tcagaacaat cacgtggtga ttcagaacga cggtacgaat
    13381 ggtctgccag gtccacagat gatgaaggcc gtgtatgaca tggcccgcaa gggtgcccgt
    13441 gatgaaattc agacacagat gcgtgatggt ggcctgttct ccggaggtgg acgatgaaaa
    13501 ccttccgctg gaaagtgaaa cccggtatgg atgtggcttc ggccccttct gtaagaaagg
    13561 tgcgctttgg tgatggctat tcccagcgag cgcctgccgg gctgaatgcc aacctgaaaa
    13621 cgtacagcgt gacgctttct gtcccccgtg aggaggccat ggtactggag tcgtttctgg
    13681 aagagcacgg gggctggaaa gcctttctgt ggacgccgcc ttatgagtgg cggcagataa
    13741 aggtgacctg cgcaaaatgg tcgtcgcggg tcagtatgct gcgtgttgag ttcagcgcag
    13801 agtttgaaca ggtggtgaac tgatgcagga tatccggcag gaaacactga atgaatgcac
    13861 ccgtgcggag cagtcggcca gcgtggtgct ctgggaaatc gatctgacag aggtcggtgg
    13921 agaacgttat tttttctgta atgagcagaa cgaaaaaggt gagccggtca cctggcaggg
    13981 gcgacagtat cagccgtatc ccattcaggg gagtggtttt gaactgaatg gcaaaggcac
    14041 cagtacgcgc cccacgctga tggtttctaa cctgtacggt atggtcaccg ggatggcgga
    14101 agatctacag agtctggtcg gcggaacggt ggtccggcgt aaggtttacg cccgttttct
    14161 ggatgcggtg aacttcgtca acggaaacag tgacgccgat ccggagcagg aggtgatcag
    14221 ccgctggcgc attgagcagt gcagcgaact gagcgcggtg agtgcctctt ttgtactgtc
    14281 cacgccgacg gaaacagatg gcgctgtttt tccgggacgt atcatgctgg ccaacacctg
    14341 cacctggacc tatcgcggcg atgagtgcgg ttatcacggt ccggcggtcg cggatgaata
    14401 tgaccagcca acgtccgaca tcacgaagga taaatgcagc aaatgcctga gtggctgtaa
    14461 gtttcgcaat aacgtcggca actttggcgg cttcctttcc attaacaaac tttcgcagta
    14521 aatcccatga cagagacaga atcagcgatt ctggcgcacg cccggcgatg tgctccagcg
    14581 gagtcgtgcg gcttcgtggt aagaacgccg gagggggaaa gatatttccc ctgcgtgaat
    14641 atctccggtg agccggaggc gtatttccgt atgtcgccgg aagactggct gcaggcagag
    14701 atgcagggtg agattgtggc actggtccac agccaccccg gtggtctgcc ctggctgagt
    14761 gaggccgacc ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg
    14821 gcgattcaca agttccgctg tgtgccacat cttaccgggc ggcgctttga gcacggggtg
    14881 acggactgtt acacgctgtt ccgggatgct taccatctgg cggggattga gatgccggat
    14941 tttcatcgtg aggatgactg gtggcgtcac ggtcagaatc tctatctgga taatctggag
    15001 gccacagggc tgtatcaggt gccgttgtca gcggcgcagc cgggcgatgt gctgctgtgc
    15061 tgttttggtt catcggtgcc gaatcatgcc gccatttact gtggtgacgg cgagctgctg
    15121 caccatattc ctgaacaact gagcaaacga gagaggtata ccgacaaatg gcagcgacgc
    15181 acacactccc tctggcgtca ccgggcatgg cacgcatctg cctttacggg gatttgcaac
    15241 gatttggccg ccgcatcgac cttcgtgtga aaacgggtgc cgaagccatc cgggcgatgg
    15301 ccacacagct ccctgcgttt cgtcagaagc tgagcgacgg ctggtatcag gtgcgtattg
    15361 ccgggcagga tgttagcacg tccggattaa cggcacagtt acatgaggct ctgcctgacg
    15421 gcgctgtgat tcatattgtt cccagagtcg ccggggccaa gtcaggtggt gtattccaga
    15481 ttgtcctggg agcagccgcc attgccggat cattctttac cgccggagcc acccttgcag
    15541 catggggggc tgccattggt gccggtggta tgaccggcat tctgttttct ctcggtgcca
    15601 gtatggtgct cggcggtgtg gctcagatgc tggcaccgaa agccagaact ccccgcacac
    15661 agacaacgga taacggtaag cagaacacct atttctcctc actggataac atggttgccc
    15721 agggcaatgt tatgcctgtt ctgtatggtg aaatgcgcgt ggggtcacgt gtggtatctc
    15781 aggagatcag cacggcagac gaaggggacg gtggtcaggt tgtggtgatt ggtcgctgat
    15841 gcaaaatgtt ttatgtgaaa tcgcctccgg gcggttttat cgtttatgga gcatgacgaa
    15901 tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc gaaggacaac ctgaagtcca
    15961 cgcagctgct gagtgtgatc gatgccatca gcgaagggcc ggttgaaggt ccggtggatg
    16021 gattaaaaag cgtgctgctg aacagtacgc cggtgctgga cagtgagggg aataccaaca
    16081 tctccggcgt cacggtggtg ttccgggcag gtgagcagga gcagtcaccg ccggagggat
    16141 ttgaatcctc cggctccgag acggtgctgg gtacggaagt gaaatatgac acgccgatca
    16201 cccggaccat cacgtcggca aacattgacc gactgcgttt taccttcggt gtacaggcac
    16261 tggtggaaac cacctcaaag ggagacagga atccgtctga agttcgcctg ctggttcaga
    16321 tacagcgtaa cggtggctgg gtgacggaaa aagacatcac cattaagggc aaaaccacct
    16381 cgcagtatct ggcctcggtg gtggtggata acctgccgcc gcgcccgttt aatatccgga
    16441 tgcgcagaat gacgccggac agcaccacag accagctgca gaacaaaacg ctctggtcgt
    16501 catacaccga aatcatcgat gtgaaacagt gctacccgaa cacggcactg gtcggcgtac
    16561 aggtggattc ggagcagttc ggcagccagc aggtgagccg taattatcat cttcgcgggc
    16621 gcattctgca ggtgccgtcg aactataatc cgcagacgcg gcaatacagc ggtatctggg
    16681 acggaacgtt aaaaccggca tacagcaaca acatggcctg gtgtctgtgg gatatgctga
    16741 cccatccgcg ctatggcatg gggaaacgtc ttggtgcggc agatgtggat aaatgggcgc
    16801 tgtatgtcat cggccagtac tgtgaccagt cggtgccgga cggctttggc agcacggagc
    16861 cgcgcatcac ctgtaatgcc tggctgacca cacagcgcaa ggcgtgggat gtgctcagtg
    16921 atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg acgttcgtgc
    16981 aggaccgaca gtcggataag gtgtgggcct ataaccgcag taatgtggtg atgccggatg
    17041 atggcgcgcc gttccgctac agtttcagcg ccctgaaaga ccgccataat gccgttgagg
    17101 tgaactggat tgacccggat aacggctggg agacggcgac agagcttgtg gaggatacgc
    17161 aggccattgt ccgttacggt cgtaacgtca cgaagatgga tgcctttggc tgtaccagcc
    17221 gggggcaggc acaccgcgcc gggctgtggc tgattaaaac ggaactgctg gagacgcaga
    17281 ccgtggattt cagcgtgggt gctgaagggc ttcgtcatgt accgggcgat gtcattgaaa
    17341 tctgcgatga tgactatgcc ggtatccgca ccggcgggcg cgtgctggcg gtgaacagcc
    17401 agacccggac gctgacgctc gaccgtgaaa tcacgctgcc atcctccggc accacgctga
    17461 taagcctggt tgacgggcag gggaatccgg tcagcgtgga ggtccagtcc gtcaccgacg
    17521 gcgtgaaggt aaaagtgagc cgtgttcctg acggtgttgc tgaatacagc gtgtgggggc
    17581 tgaagctgcc gacgctgcac cagcgattgt tccgctgcgt gagtatccgt gagaacgacg
    17641 acggcacgta tgccatcacc gccgtgcagc atgtgccgga aaaagaagcc atcgtggata
    17701 acggggcgca ctttgacggc gaccagagcg gcacggtgaa tggtgtcacg ccgccagcgg
    17761 tgcagcacct gactgccgaa gtcaccgcag acagcgggga atatcaggtg ctggcgcgct
    17821 gggacacgcc gaaggtggtg aagggcgtga gcttcctgct ccgtctgacc gtaacagcgg
    17881 atgacggcag tgagcggctg gtcagcacgg cccggacgac ggaaaccact taccgcttca
    17941 cacaactggc gctggggaac tacaggctga cagtccgggc agtaaatgcg tgggggcagc
    18001 agggcgatcc ggcgtcggta tcgttccgga ttgccgcacc ggcagcgctg tcgcggattg
    18061 agctgacgcc gggctatttt cagataaccg ccacgccgca tcttgcggtt tatgatccga
    18121 cggtacagtt tgagttctgg ttctcggaaa agcggattgc ggatatcagg caggttgaaa
    18181 ccacagcacg ctatcttggc acggcgctgt actggatagc cgccagtatc aatatcaaac
    18241 cgggccatga ttattacttt tatatccgca gtgtgaacac cgttggcaaa tcggcattcg
    18301 tggaggctgt tggtcagccg agtgatgatg catccggtta tctggatttt ttcaaaggcg
    18361 agatagggaa aacccatctg gctcaggagc tgtggacgca gattgataac ggtcagcttg
    18421 cgcctgacct ggctgaaatc aggacgtcca ttacggatgt cagcaatgaa atcacgcaga
    18481 ccgtcaataa gaaactggaa gaccagagtg cggcaattca gcagatacag aaggttcagg
    18541 ttgatacaaa taataacctg aacagcatgt gggctgtgaa gctgcagcag atgcaggacg
    18601 gacgccttta tatcgcgggt attggtgccg gtattgagaa cacccctgac ggcatgcaga
    18661 gtcaggtgct gctggcggcg gacaggattg cgatggttaa tcctgcgaat ggcaacacaa
    18721 aaccgatgtt tgttggtcag ggcgatcaga tattcatgaa cgacgtgttc ctgaaacgcc
    18781 tgacggcccc caccattacc agcggtggaa atccatcggc attttccctg actccggacg
    18841 gaaagctgac cgctaaaaat gcggatatca gtggcagtgt gaatgcgaac gccgggacgc
    18901 tcaacaatgt cacggtaaat gaaaactgta cgattaaggg catgctggag gcgactcagg
    18961 tcagaggtga cttcgttaaa gctgtatcca aatcatttcc gaaacaggct ggtacgtggg
    19021 gtaacacgga aacaccaaac gggacggtta cagtcaccat aagcgatgat cataactttg
    19081 accgccagat tattattccg cccattattt ttaacggtat agcgtatgac gatccgggga
    19141 gcggaaataa cccaggaggc acgcgataca cgggttatgg ttttgaagtt cgcaaaaacg
    19201 gcgtattaat cgcatccaga gaaactaaag gtgccattcc cggtagttac agtgcagtta
    19261 ttgatatgcc tagtggtggt ggtagcgtca ctctggagtt taagattttc cagaaaggca
    19321 atcagggggc aggcaatatc accgactgta cggtgattgt gaccaaaaaa gcggcttccg
    19381 gcatcagtat tcgttgaaat atttataacc ccaataaatg gcgtcaggaa tgacgccttt
    19441 tttattgcag aaaagcgaga ggtaattatg cgtaaagttt gtgcagccat tttgtccgca
    19501 gccatttgcc tggccgtatc cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg
    19561 agcgcggggt atcttcatgc ccggaccaac gttcccggca gtgatgatct gaacgggatt
    19621 aacgtgaaat accgttatga gtttacggat acgctggggc tggtgacgtc attcagctat
    19681 gcaggagaca agaatcgcca gctgacccgt tacagcgata cccgctggca tgaagattcc
    19741 gtgcgtaacc gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa tgaatggttc
    19801 agcgcgtatg cgatggcggg tgtggcttac agccgtgtgt cgactttttc cggggattat
    19861 atccgcgtaa ctgacaataa ggggaaaacg cacgatgtgc tgaccggaag tgatgacggt
    19921 cgccacagca acacgtctct ggcgcactga cggtttcatc gtgggtgtcg gttataaatt
    19981 ctgattagcc aggtaacaca gtgttatgac agcccgccgg ttcaggcggg cttttttgtg
    20041 gggtgaatat ggcagtaaag atttcaggtg tactgaaaga cggcacagga aaaccggtac
    20101 agaactgcac aatccagctg aaagcaaaac gtaacagcac cacggtggtg gtgaacacgc
    20161 tggcctcaga aaatccggat gaagccgggc gttacagcat ggacgttgag tacggtcagt
    20221 acagcgttat tctgttggtg gaaggattcc cgccgtcaca tgccgggacc atcaccgtgt
    20281 atgaagattc ccgacccggt acgctgaatg attttctcgg tgccatgacg gaggatgatg
    20341 cccgtccgga ggcactgcgc cgttttgagc tgatggtgga agaggtggcg cgtaacgcgt
    20401 ccgcggtggc acagaacacg gcagccgcga agaaatcagc cagcgatgcc agcacatcag
    20461 cccgtgaggc ggcaacccat gcgactgatg ctgcagattc agcacgtgca gccagcacgt
    20521 cagccggaca ggccgcttcg tcggctcagt cagcgtcttc cagcgcagga acggcatcaa
    20581 caaaggccac tgaagcctca aaaagtgctg ccgctgcaga gtcctcaaaa agcgcggcgg
    20641 ctaccagtgc cggtgcggcg aaaacgttag aaacgaatgc ggcagcgtca caacaatcag
    20701 cagccacttc tgcatccacc gcgaccacga aagcgtcaga agctgccacc tcagcccggg
    20761 atgcgtcggc ttcaaaagag gcggcaaaat catcagaaac gaacgcatca tcaagcgcca
    20821 gtagcgcagc ttcctcggcg acggcggctg caaattctgc gaaggcggcc aaaacgtccg
    20881 agacgaacgc caggtcttct gaaacggcag cgggacagag tgcctcagct gcggcaggct
    20941 caaaaacagc ggctgcgtcg tctgccagcg ccgcgtcaac aagtgccggg caggcctcag
    21001 ccagtgccac cgccgccgga aaatcggcag aaagcgccgc atcatccgct tcaacagcca
    21061 caacgaaggc tggcgaagcc actgagcagg ccacggcagc agcgaggtca gcttccgcag
    21121 cgaagacatc cgaaacgaac gcgaaagcgt cggaaacccg tgcagaatcc tcaaaaacgg
    21181 ctgccgcatc gtccgccagt tcggcggcgt catcggcatc atcggcgtct gcttcaaaag
    21241 atgaggcgac cagacaggcg tcagcagcga agggcagcgc cacgacggca tccacgaagg
    21301 cgacagaggc tgctggtagt gcgacggcag cagctcagag caaaagtacg gcggaatctg
    21361 cagcaacgcg cgctgagaca gcggcaaaac gggcagagga tattgcatcc gccgtggcgc
    21421 ttgaggatgc gagcacgacg aaaaagggga tagtacagct cagtagtgcg actaacagca
    21481 cttccgagtc actggcggca acaccaaaag ccgttaaggc cgcgtatgac ctggctaacg
    21541 ggaaatacac cgcacaggat gcaacgacag cacagaaagg gataatccag ctaagcagcg
    21601 cgaccaacag cacgtctgaa acgctggcgg caacgccaaa ggcagtaaaa gcagccaatg
    21661 acaatgctga gaaacgtctg cagaaagatc agaacggtgc ggatatccct ggcaaagaca
    21721 cctttacgaa aaatattggt gcctgccgtg ccttcggtgg gtcagtaagc acaacaacag
    21781 gaaactggac gactgcacag tttatcgagt ggctggattc tcagggagca tttaaccatc
    21841 catactggat gtgcaagggt tcctggtctt atggcaataa taaaatcatt actgatactg
    21901 gctgcggtaa tattcatctc gccggagctg tcattgaagt aatggggata aagtcagcga
    21961 tgacgatccg cattaccact ccgaccacat ccagcggtgg cggaacaacc aacgcccagt
    22021 ttacctatat taatcacgga acagattatt cacctggctg gcgaagggac tataactcca
    22081 gaaataagcc aacggcatca gagatcgggg cgctaccgtc aggtggaaca gcagtatcat
    22141 cagttaatct ggcttcaaaa ggtcgggtag ccgcgctgac agacaatacg cagggggcag
    22201 caggtcttga gttatacgag gtgtataaca acggatatcc aacagcgtat ggaaatatca
    22261 ttcacctgaa agggatgaca gccgttggcg aaggtgagtt actcatcggc tggagtggta
    22321 caagcggtgc tcatgctccg gcatttattc gttcacgacg ggatacgacc gacgcaaact
    22381 ggtcgccgtg ggcgcagctt tacacctcgg ctcatcctcc tgcagagttt tatccagtcg
    22441 gtgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg atgcaggggc
    22501 agacttttga caaatctgct tacccgaaac ttgcagccgc ttatccgtca ggcgtgatcc
    22561 ctgatatgcg tggctggacg attaagggca agcccgccag tggtcgtgcc gtattgtctc
    22621 aggaacagga tggcattaaa tcgcacaccc acagcgccag cgcatccagt acggatttgg
    22681 ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac accggggcgc
    22741 atacgcacag tctgagcggc tctacaagtt cagctggggc ccaccaacat agtcagactg
    22801 ggcccagaac gaatagcggg agtcaaccta ctggaatgtt tccggcaggg agtactcaag
    22861 ttagcgggac taatcaggtt ggtatcagtg gctcattaac cagtggcacc tcccaatggg
    22921 taggtaaatc atcttcagaa ggaaaccata cacactcact gtccggcaca gcagcatctg
    22981 cgggtgcaca cgcgcatact gtcggtattg gtgctcatac gcactctgtt gcgattggtt
    23041 cacatggaca caccatcacc gttaacgctg ctggtaacgc ggaaaacacc gtcaaaaaca
    23101 tcgcatttaa ctatattgtg aggctcgcat aatggcattc agaatgagtg aacaagcacg
    23161 gaccataaaa atttataacc tgctggccgg aactaatgaa tttattggtg aaggtgacgc
    23221 atatattccg cctcatacag gtctgcctgc aaacagtacc gatattgcac cgccagatat
    23281 tccggctggt ttcgtggctg ttttcaacag tgatgagtca tcgtggcatc tcgttgaaga
    23341 tcatcggggt aaaacggttt atgacgtggc ttccggcgac gcgttattta tttctgaact
    23401 cggctcatta ccggaaaatg tcacctggtt atccccggaa ggggagtttc agaagtggaa
    23461 cggcacagcc tgggtgaagg atacggaagc agaaaaactg ttccggatcc gggaggcgga
    23521 agaaacaaaa aacagcctga tgcaggtagc cagtgagcat atagcgccgc ttcaggatgc
    23581 tgtggatctg gagatcgcaa cggagaaaga aaccttgttg ctggaagctt ggaaaaagta
    23641 tcgggtgttg ctgaaccgtg ttgatacatc aactgcaccg gatattgaat ggccagtagc
    23701 acctataggg taaattcgta atgattacct aaatacgtta ttcttttgtt aaaaaagtga
    23761 ttttgctata ggtagggagt atgttcttgg attaagaata aaaaatcgcc atgaaagagc
    23821 ttcatggcga gtacataaac gtacaaaatg tgatgaaagt agagtggcgt aagccactat
    23881 attaagatat gacaatagcg aagaaaagta aataacattt tatggacgtt taattgttaa
    23941 ttataaaaac tagagtgccc gatattgttt atcttgcttt aaaataatgt actcaaacca
    24001 aatttttgag ctaaaagctc aaataatata aatgcaataa taatgtttaa taagacaatt
    24061 agcttgcttt gtattttcat ttcggaatac tgttaagtaa ctttgatgac cctaacaata
    24121 gatcggaatt ttcttagagt caatggtcac tgtttttgct tgcaaagcaa atgttttggt
    24181 ggttataagt cgtcttaata tagtcacgca tgcaacttgt tccatgcgga gattaaagat
    24241 tggaatataa cgcgaagatg gaattaaatt acatttgcaa taattaattt attattactc
    24301 aataattacc gatattatta ttgtaaatta atcattagaa tgcaaaaaga aatgtaacgg
    24361 caaaataaga ggacgatagc tgagaaaaaa gcgtgctttc ccaatctggg gcaaatcacg
    24421 tctgaatgac acctgaaaac aggtggtagc ctcaagaa
//
LOCUS       DAJVYM010000051        23848 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVYM010000051 DAJVYM010000000
VERSION     DAJVYM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.051"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(74..1327)
                     /gene="amiC"
                     /locus_tag="OQS60_001906"
     CDS             complement(74..1327)
                     /gene="amiC"
                     /locus_tag="OQS60_001906"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS2387673.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1559..2890
                     /gene="argA"
                     /locus_tag="OQS60_001907"
     CDS             1559..2890
                     /gene="argA"
                     /locus_tag="OQS60_001907"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS2387674.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2952..4778)
                     /gene="recD"
                     /locus_tag="OQS60_001908"
     CDS             complement(2952..4778)
                     /gene="recD"
                     /locus_tag="OQS60_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS2387675.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4778..8320)
                     /gene="recB"
                     /locus_tag="OQS60_001909"
     CDS             complement(4778..8320)
                     /gene="recB"
                     /locus_tag="OQS60_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS2387676.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="OQS60_001910"
     CDS             complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="OQS60_001910"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS2387677.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11377..14745)
                     /gene="recC"
                     /locus_tag="OQS60_001911"
     CDS             complement(11377..14745)
                     /gene="recC"
                     /locus_tag="OQS60_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS2387678.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14758..15081)
                     /locus_tag="OQS60_001912"
     CDS             complement(14758..15081)
                     /locus_tag="OQS60_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS2387679.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15066..15473)
                     /locus_tag="OQS60_001913"
     CDS             complement(15066..15473)
                     /locus_tag="OQS60_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS2387680.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15470..16033)
                     /locus_tag="OQS60_001914"
     CDS             complement(15470..16033)
                     /locus_tag="OQS60_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS2387681.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16024..16494)
                     /locus_tag="OQS60_001915"
     CDS             complement(16024..16494)
                     /locus_tag="OQS60_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS2387682.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16679..17473)
                     /gene="thyA"
                     /locus_tag="OQS60_001916"
     CDS             complement(16679..17473)
                     /gene="thyA"
                     /locus_tag="OQS60_001916"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS2387683.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17480..18355)
                     /gene="lgt"
                     /locus_tag="OQS60_001917"
     CDS             complement(17480..18355)
                     /gene="lgt"
                     /locus_tag="OQS60_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS2387684.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18506..20752)
                     /gene="ptsP"
                     /locus_tag="OQS60_001918"
     CDS             complement(18506..20752)
                     /gene="ptsP"
                     /locus_tag="OQS60_001918"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS2387685.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20765..21295)
                     /gene="rppH"
                     /locus_tag="OQS60_001919"
     CDS             complement(20765..21295)
                     /gene="rppH"
                     /locus_tag="OQS60_001919"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS2387686.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            21980..22669
                     /gene="mutH"
                     /locus_tag="OQS60_001920"
     CDS             21980..22669
                     /gene="mutH"
                     /locus_tag="OQS60_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS2387687.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22738..23451
                     /locus_tag="OQS60_001921"
     CDS             22738..23451
                     /locus_tag="OQS60_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS2387688.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23589..23807
                     /gene="ygdR"
                     /locus_tag="OQS60_001922"
     CDS             23589..23807
                     /gene="ygdR"
                     /locus_tag="OQS60_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS2387689.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5699 a         6526 c         5960 g         5663 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctactct cccataaaaa aagcgccatt
       61 cagcgccttt ttatcatccc cttctcgcca gcgttgcccc atcggcaaaa tacgctttaa
      121 tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
      181 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      241 ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg attttaccga
      301 gcttattcag cactgcttta ccaaacttaa ggctgtcagc aatggtcagc gactgcacca
      361 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt
      421 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgca cctttggttg
      481 agagcgcaaa caccgaggaa ccgctcggct ggcgactggt aaaggcgtcg gcatggatag
      541 agacaaacag gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
      601 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc
      661 gcaaacggcg agctatttgc aataccacat ctttttcgcg cgttttgtat ttccccaccg
      721 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc
      781 ctgctttacc tggttgtgga ccactttgcg ctggcggcac ctgcttttcg aggtcgcctt
      841 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
      901 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
      961 gttttacgtt ttgctttaat tcaaaaacca tccgtacggt ttgcgggtca aattgcccga
     1021 cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta
     1081 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcagggtta ctcaacgcga
     1141 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1201 gccagacgcg caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca
     1261 atagccacat agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     1321 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt
     1381 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt
     1441 ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc
     1501 tgcgaaaaaa cagaataaaa atacaataat ttcgaataat catgcaaagg ggtgtgccgt
     1561 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     1621 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     1681 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     1741 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct
     1801 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
     1861 gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata acacgccgct
     1921 gcagggcgcg catatcaacg tcgtcagtgg caattttatt attgcccagc cgctgggcgt
     1981 cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat
     2041 ccgtcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac
     2101 tggcgagagc tttaatctga cctcggaaga gattgccact caactggcca tcaaactgaa
     2161 agctgagaag atgattggtt tttgctcttc acagggcgtc actaatgacg acggtgatat
     2221 tgtctcagaa cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg
     2281 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     2341 cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
     2401 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     2461 aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
     2521 tctggtgcgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
     2581 gctcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg
     2641 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
     2701 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     2761 cacgcgcagt attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact
     2821 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     2881 tttagggtaa gcgatgaaaa tcgccggatg cggcaggcgt aacactcgtg cgtcgcatcc
     2941 ggcaattacg tttatccccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
     3001 cgagtggcga ttgccgcact taatatacgc tcatcggcat acagcgacag acggcgacgc
     3061 gcgcgggtca ccgcggtgta taccagctct cgcgttacta tcggcgtgcg ttggctcggt
     3121 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
     3181 gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
     3241 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
     3301 aacccaagcg cgctgtcatt acgggcaatc atcaccggtc gaccttcgta ccaacgagag
     3361 tgcgaatgac gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt
     3421 ccagccacgc caaatggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc
     3481 tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc
     3541 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag ccgtttttca
     3601 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc
     3661 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
     3721 cagagactgt cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagttccc
     3781 gtcaggcggc ttagctgccc ggcacgctcg gcggtaaagc cggcattggc ataagcgcag
     3841 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
     3901 atcactcgcg catgatcggg caaggcgtcg atcagtctcg acatcatagg cagatcgatc
     3961 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
     4021 aaacgctggc tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga
     4081 atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg
     4141 gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
     4201 tcggccattt gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct
     4261 gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgctgccgc cactttttgc
     4321 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca
     4381 tcaacctcaa tggcatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac
     4441 cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc
     4501 ctgctgaccg cttgagaagc cagcaaggat tcttcccaat tttgtagctc accgatttca
     4561 ctgacacagg tcgccaacag cggatgcgac gcctcgttat tttccagtcg tgaaagcggc
     4621 aaacagacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca
     4681 ggatgttcat ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg
     4741 tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac
     4801 cggcaaacat ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc
     4861 cttgttgcgg atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt
     4921 gacgatcata gtcgtaatca gcaatgcgat ggcgcagata gcgatgcagc gccagggtat
     4981 aaagctgata ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg
     5041 tgtaagccga actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc
     5101 cttcgtggcg gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact
     5161 ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc
     5221 tggcgatgag cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt
     5281 cggaaagctg actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga
     5341 tccactcggt caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct
     5401 gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga
     5461 atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
     5521 cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
     5581 gaccacgctg ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
     5641 gtagtgtcct cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat
     5701 caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc
     5761 gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt
     5821 ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac
     5881 tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac
     5941 gttcgacctc cgcgaggtcg acgctttctg gcgcagcatt aagatccaaa actgcctcaa
     6001 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
     6061 accagaccag cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat
     6121 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
     6181 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa
     6241 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
     6301 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
     6361 tgcgccagat ttgccgataa ccatcgaact cttcgactac cgcatcccac gcatgttcgt
     6421 cattattcag cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac
     6481 tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacagccaa agcatttcct
     6541 gcgcttccag agtttcaaaa acactatcgc ggttcgaaag gtaaacggaa gggatttcca
     6601 gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacgctga
     6661 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct
     6721 gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
     6781 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
     6841 cacctttaaa gacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg
     6901 gtatttcgcg aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acactgttca
     6961 ccattccagg tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac
     7021 gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt
     7081 cgccaatcag caacaatgcg gtttctggct gatggtgcca gatacggcga aaaattcggt
     7141 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
     7201 tcgccgctgc caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca
     7261 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
     7321 cagccaatgc gcgggtgatc accagatcac ggatcgacaa tggttctgca agcagttgat
     7381 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggctttcgtg cgatcttcta
     7441 agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt
     7501 ctgcccaggc gctgatcttg tcgatccatt tagcctgatt gctacggtta aacttgcgtc
     7561 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
     7621 gttttaccgt atcaatacgc gccacaattt gcgcgtgacg ggaagctagc gtttcatcat
     7681 cgggcggcgg tgctttgata accggcgctt cgccttgcag ataacgatta atatcgcgca
     7741 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg
     7801 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt
     7861 caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct
     7921 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca
     7981 accactgcgc ggcttgcgct ttatcgtcga cctcttccag caggtgttcg tacaatggat
     8041 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
     8101 cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca
     8161 gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa taaagcgccg
     8221 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
     8281 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
     8341 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
     8401 tcggctttcc cgttttggct gccggaaatc tgcgacagga tagccatgcc ttgcggctcg
     8461 actaccgcct gatggaagaa atcggccagt ttttgcggcg tcagcagttt aatctgggcc
     8521 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
     8581 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg
     8641 atttgcgcaa actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac
     8701 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
     8761 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct
     8821 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
     8881 gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg
     8941 gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg
     9001 tttcgacacc actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt
     9061 gttgcctggg cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt
     9121 aaggcgtcgc gataagccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt
     9181 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
     9241 aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
     9301 agctgatctt ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc
     9361 ggcagacgct gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa
     9421 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
     9481 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
     9541 aaaatcaggc tgacatcagc tttgggttcg ctggaaaaat aacggcttgg cgcatacacc
     9601 acgcgcagat tcgactcatc aacaatcagc tctggatggt catatttctt ctctgactta
     9661 atcagcgaga aatcgtcagg aatatagggg ttaagctccg gtaaggagag cgcaatattg
     9721 gcagcttttt gctgccagtc agcgaaagtt tgttcgctga ttttatcgac ctgatacggc
     9781 gcatcgacaa aataagccgt tttattgtgc ggctctttcg ggctgatata ccagatacgc
     9841 gcattctgcg gcgtcatcat cgccagacgt tcttttactg ctttagcatc gtaccgatcg
     9901 gcaatattga ctgcatccag cgtatgctca accggaacgc gaatcatggt atctgccagc
     9961 cattcgacgt aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca
    10021 ttcgccagtt catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag
    10081 ctaaaaattg ccgccacaac ctgatcgcga ttagccaggc ctttatcggt taaagacgca
    10141 gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc
    10201 tcaactaatc cctgcttttg cagccagtcg gaaagtgtac ctgggctgcg attaccaatc
    10261 agataggtaa tcaattcatc cgttttacta cggaacttcg ctgagttgtt atcgatgcga
    10321 aactcaacgc gcagaacttt acgcggcatt gccgggacgt aatgaatgat aatgcccttt
    10381 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    10441 cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt gctgtaaata
    10501 accgccttca tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc
    10561 accggattac caggtttatc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    10621 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    10681 cgcgccatgg ttaattcagc gttcaccgca ttacgctcac gttcggcata tttcttgtcg
    10741 agcaaaggtt cagcaatagc atcggccagg cggtctaccg caccaggcaa ggcgtcgttc
    10801 tcaacttcca gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg
    10861 tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga
    10921 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca
    10981 acgggcacca ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc
    11041 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    11101 atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca
    11161 actaacaaca ataatgcttt gaaccaggtg ctgcggggca ttctcggacc tcataatcaa
    11221 cttaattttc tgtccagatt caacacgtta acgcaaatca tctgccagaa tttaatcttg
    11281 tgctgcacga gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa
    11341 agtaagcgga tagattgcgc aatttttata cagcactcat gactgattaa agcgaaacag
    11401 cggtaacagg aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg
    11461 ccgccagagc ctttgatacc agatatcatc accttcgcca cgcaccatca tgttaccttc
    11521 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    11581 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    11641 caatggcgcg gacatccctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    11701 ctgttcggct gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact
    11761 ttcaccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    11821 cgccacactt aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa
    11881 ccagccagtt atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg
    11941 gcgacaggca atgactctgt cggcaagctg ctgcatctcc tggcactgag tttcccagaa
    12001 aatttcacca aaagcgccat acggtaaatc ccctgccgcc cggaagcgac ggaacaagcg
    12061 ttcggcatca tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct
    12121 aagcccttcc agaataaatg gctcggtgtc ggggatttcg ctgtcttcag tacggaagtt
    12181 cacctgcaaa cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt
    12241 ttccagcggc acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc
    12301 tttaccagcc tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg
    12361 ctgatagttt tgtggatcaa acggcattcg ggtatggagg caagtaagat gcgcttttac
    12421 ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc
    12481 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    12541 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    12601 cagatagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    12661 gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    12721 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc
    12781 gagaaaacgc tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag
    12841 tgacagcggc accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca
    12901 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgct
    12961 gagcatatcg cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg
    13021 acgccagatg tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    13081 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    13141 gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag
    13201 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    13261 ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac
    13321 atccagtaat gccagcacgt cttccgacac aaagcggctg tctggcaacg ataacaggct
    13381 gataaacgct tccagtaccg gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg
    13441 caggtaacga tccgcaggtg cgctaccaaa cacagcccga ataaacggac tgtagctgtc
    13501 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cttccagcat
    13561 cgccagcagg cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    13621 ggtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    13681 gatgttcaca ccagcaacgg cacggttttc cagttccaga atgtcagact gaatgttatg
    13741 cagcaggtta tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc
    13801 agaaaggaga taaatgtagt cgcgcccaag cttaccccat gaagccagca gcgggttgcc
    13861 gacatcctgt tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cacgaaatag
    13921 cggcaattcg cgatcttcaa aactgtgtcg acgctgacgg gtcagcagtt tcgccagata
    13981 agcaggatct ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    14041 ttcaatatgt ttacccagcg cctgtagcgc ctggagataa acaggcggta acgcggaaat
    14101 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    14161 ttcgataaag cgctgataga gattggcgcg gtgccagcgc ggttgcccga gttgatgggt
    14221 atattccacc agcgccttcc acaacggggc ttgccaggcc tgtgcttctc ccagcccttc
    14281 aaccaaatgt cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc
    14341 aaacaggtcc gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt
    14401 cagataatgc cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat
    14461 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac
    14521 ccggacgaac atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat
    14581 actaaacttt tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag
    14641 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    14701 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    14761 ctgacgattc gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag
    14821 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    14881 ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg
    14941 gtactggttt ctgctggcga gactgttcat taatgttcgc tggatacccg ataacgcagt
    15001 gacaatcatc accatcagca ccatcgcaac cattacctcc ggcaggctaa agccttgttg
    15061 attcttcagg gaagctgaca taacgccctc tctttcagcg gacaaaaatc gctccagccg
    15121 cgtggcgaaa aaacaatgtt tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct
    15181 tcataaccgg caaccaataa ggcttcatta tctgccagta aacgcaaaca aacccgggca
    15241 tcggttccag cgtactgcga gcactgaact gcgggctgcg tctgccagga gtgcattttc
    15301 ccccacgcca gcgccgactg aacgatggcc tggcggcgca atgactgact ttctatgctc
    15361 acgcgagagg caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa
    15421 accagcagca tcaggaccag agccagtgac gaaacacctt tttcgcggtt cacaggttga
    15481 atcctgtcac gctatagctg gcatccacca cggtttgcgg ttcagactta ctggcagcac
    15541 gcatattaac cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag
    15601 tgtcgataat gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg
    15661 tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcagact
    15721 cttttaccgg ttcgcgatcc cagataccgt tactgttcgc atcccactgc acaatgatac
    15781 agtcaccctg tccgacaatt tccagccctt cgccggtaca gctgccatga caataacccg
    15841 ccctctggag atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca
    15901 gcttacgggt gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca
    15961 gcaacaatac gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct
    16021 cttttacagg catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga
    16081 aaccaccagc caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt
    16141 attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca
    16201 gcgtggcaca aagaccaatg gtgaactgcc atgacaggta ttggccccag cagcggaact
    16261 cacaaggcac cataacgtcc cctccctgat aacgctgata ctgtggtcgc ggttatgcca
    16321 gttggcatct tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccatag
    16381 ccgttgcgac tgctgccagt attgccagcc atagagtcca cttgcgctta gcatgaccaa
    16441 aatcagcatc gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc
    16501 agtatggagc gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc
    16561 ggactattta cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta
    16621 acggagggta aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt
    16681 agatagccac cggcgcttta atgcccggat gtggatcgta gccttcaatc tcaaagtctt
    16741 cgaaacggta gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac
    16801 gcggttcgcg gcttaattgc agatgagttt gatccatatg attgctgtac aggtgcgtat
    16861 cgccaccggt ccagacaaaa tcacccactt ccagatcgca ctgctgcgcc atcatatgca
    16921 ccagtaacgc gtagctggca atgttgaacg gcaggccgag gaagacgtca caggagcgct
    16981 gataaagctg gcaagagagt ttgccgtctg ccacatagaa ctggaagaat gcatggcacg
    17041 gtgccagcgc cattttatcc agttcgccta cgttccacgc tgaaacaata atgcggcggg
    17101 aatccgggtc gtttttcagc tggttcagta ccgtagtgat ctggtcaata tgacgaccat
    17161 ctggcgttgg ccaggcgcgc cactgtttac catacactgg tccgaggtcg ccgttttcat
    17221 cggcccattc gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc
    17281 cctgcagaaa ccacagcagt tcgtggatga tggaacgcag gtggcaacgt ttagttgtca
    17341 ccagcgggaa tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc
    17401 cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta
    17461 aatactgttt catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga
    17521 ccatcatgat cacacccgcg acaatcatcg ggatggaaag aatttgcccc atgctgatgt
    17581 actgcaccca ggcaccggta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc
    17641 gaaacgcgcc gtagccaatc aggaacaagc cagagacagc tcccattggg cgcggtttac
    17701 gaatatacag gttgaggata ataaacagca ccacaccttc cagcagcagc tcgtaaagct
    17761 gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg
    17821 tttgcagcag caaaatatct tctgtacggg agcctgggaa cagcatggca aacgggaagt
    17881 tcgggtcaac gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcaccaa
    17941 gaccaaacgg aatgagtggt gcgataaaat cagaaacctg gaagaaggaa cgtttagtac
    18001 ggcgggcaaa gataatcatc accacgataa cgccaatcag gccgccgtgg aaagacatgc
    18061 cgccgtccca gacacggaac agatacagcg gatcggccat aaactgcggg aaattgtaga
    18121 acagaacata accaatacgt cccccgagga agacgccgag gaagcccgca tagagtaagt
    18181 tttcaacttc atttttggtc cagccgctgc ccggacgatt cgcccgtcgt gttgccagcc
    18241 acattgcaaa aatgaaaccc accagataca tcaggccgta ccagtgaagc gccacgggtc
    18301 ctattgagaa aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc
    18361 accacaagtt cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt
    18421 tcaggcgctc caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg
    18481 ttaaaagata tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc
    18541 gacgctccat aaaggctgca acctgatggc gaacttcggt cgccagttgc gcttccagac
    18601 tacgctgcgc aagattttct gcttcggcat aatcaatgcg ccgcagcagg tattttaccc
    18661 gcgctacaga acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca
    18721 cgcacatggg atcgcccgcc atttcaccgc acaaacggag atcgattcca tgtatttccg
    18781 cttcccgggc gatcatcgcc agagctcgta acattgcagg atgaagactg tcataaatgt
    18841 tcgccacccg ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc
    18901 caacagagat gaaatcgacc cgctttgcca gatgcggcag cataaatacc attgacggca
    18961 cttccagcat gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt
    19021 cacgtccggc acgttcaatc aggcggcgtg cttcatcaac ttcatcgagg cttgtgacca
    19081 tcggcaacag aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct
    19141 ggatcaagaa gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg
    19201 gattctcttc gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac
    19261 gcaaggtgac gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt
    19321 cttcttccga cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccga
    19381 tgccatcaat acggctgccc agtttttctt catgttccgg gcttaaacca gcattgagca
    19441 tgacttttat acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca
    19501 gacggctaag ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct
    19561 ccggatcgac cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca
    19621 ccgaaggctg aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg
    19681 cagcatggga gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca
    19741 gctcagcaag agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg
    19801 cgttcggccc ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta
    19861 aatcgccagc ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt
    19921 tttcaatgac cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca
    19981 attcgcgacg cagccgggtg tccgaaagta ggtgcgagta aagatcgaaa atagccgccg
    20041 tttctttttg tgctccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt
    20101 cttccagcgc cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat
    20161 acacctgttc cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag
    20221 gtgctgccgg taatgcgcgg attcgcgtct ggcgatattg cccaaacaag gcagtcaact
    20281 gcgactgaga aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac
    20341 tttcgtcata ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac
    20401 gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt
    20461 atttgaagct ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa
    20521 ccaggccgac gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca
    20581 gcccccgggt cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac
    20641 agacctcggt atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca
    20701 ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg
    20761 tgacttaacc tcttttacgt cgataagcag atgtgttttg tggttttggc gtattttcct
    20821 gcagtgacat caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa
    20881 atgacaccac ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg
    20941 gtgtactgct ggtttgcata ttgatttctg catcgccgct caccagctgc aagagaaacc
    21001 atttttgttt ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta
    21061 atttgtagcg caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc
    21121 ctacttcttc aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc
    21181 cgccttgcgg aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct
    21241 ggcgattaca aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac
    21301 tacctcaaaa taaagcttta tatacgaatg attgtttcat actccaagaa gacggtaaac
    21361 cactctctgc agggcattac acactaataa caattgaata actcacagtt atgtgcagag
    21421 ttataaacag aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg
    21481 tgaagaacta cggaattact acgggaaaac ccggagcatt ctgaataagc ccgatataga
    21541 cattttaaaa tattcattat tagtcaatgc attacatgta atttgagtaa tgaaaaagag
    21601 taattcgtga cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg
    21661 tatttatcca cagaatgtgc cactaagtta agcactgaac cactaaaaac tggagtttcg
    21721 tcgcacgtca aggctgtaaa tggaaacagt agtggaggtt tttcacagtt atcccagctt
    21781 tctgtggata acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaaccta
    21841 ttgcagtgtt gcgcaactcg attaccggca gcctaaaaag caatataaat cagagaattg
    21901 aacaacacat gtggaaaaag ttacactgcg aatattcggc acataattgc tgtttgtttt
    21961 ttaaccaagg tatcatgaca tgtcccaacc tcgcccactc ctctctcctc ccgaaactga
    22021 agaacagttg ttagcgcaag cacagcaact ttctggttat acattgggag aactggcggc
    22081 acttgccggg ctggttacgc cagagaattt aaaacgcgat aagggctgga ttggcgtgtt
    22141 actggagatc tggctaggtg ccagcgcagg gagtaaacct gagcaagatt ttgctgctct
    22201 gggcgtggaa cttaaaacta tccctgtgga tagtcttggt cgtccgctgg aaacaacatt
    22261 cgtttgtgtt gccccgttaa cgggtaatag cggggtgacc tgggaaacca gccatgtgcg
    22321 ccacaagctc aaacgcgtac tgtggatacc ggttgaaggt gagcgcagca tcccgctggc
    22381 gcagcgtcgc gtaggatcac cgttgctgtg gagcccgaat gaagaggaag accgacagct
    22441 acgcgaagac tgggaagaat taatggatat gattgttctc ggtcaggttg agcggatcac
    22501 cgctcgtcac ggggagtatt tacagatacg accgaaagca gcgaatgcga aagcgcttac
    22561 cgaagccatt ggtgcccggg gcgaacggat tctgacgctg ccacgcggct tttatttgaa
    22621 gaagaatttc accagtgcac tactggcccg tcattttctg atccagtagc catcgctttg
    22681 acctgccgct ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg
    22741 ttatttgcat ggataaccga tcctaacgcc tggcttgcgc tcggtacgct gacgctgctg
    22801 gagatcgttc ttgggatcga caatattatt ttcctttctc tggtggtggc aaagctcccc
    22861 acagcacaac gtgctcatgc gcgccgtctg gggttggcgg gagccatggt tatgcgtctg
    22921 gcgctgctgg catcaatcgc ctgggttacg cgcctgacaa atccgctttt tacaatattc
    22981 agtcaggaaa tttccgcccg tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg
    23041 aaagccagca aggaaatcca cgaatccatt gaaggtgaag aagaagggct gaaaacacgc
    23101 gtttcatcat tcctcggcgc tatcgtgcag attatgctgc tggatattat cttcagcctc
    23161 gactcggtga ttaccgctgt gggtctgtca gatcacctgt ttattatgat ggccgccgtg
    23221 gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc
    23281 catccttcgg taaaaatgct ggcgctctct ttcctgattc tggtcggctt taccctgatt
    23341 ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct
    23401 attgcggttg aaagcctcaa cctgattcgc aacaaaaaga acccgctctg atactccgta
    23461 cgctctcctg cgggagggcg tactcactcc tgttaccctc acagtttaag attttcctgc
    23521 tttcaagata tataacgtcg gtttataaac agactattat cataggtggg ccaaatgagg
    23581 ataaccggat gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt
    23641 ccgggtgttc cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg
    23701 gaaaacctga aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg
    23761 cgatgcaaat taaccgtgat gatgtttcgc aaattattga acgttaacaa ataaggtcag
    23821 catccggctg taatgccgat cagttaag
//
LOCUS       DAJVYM010000052        23420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVYM010000052 DAJVYM010000000
VERSION     DAJVYM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.052"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..744)
                     /locus_tag="OQS60_001923"
     CDS             complement(<1..744)
                     /locus_tag="OQS60_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS2387690.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFD"
     gene            complement(756..1850)
                     /locus_tag="OQS60_001924"
     CDS             complement(756..1850)
                     /locus_tag="OQS60_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS2387691.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(1883..2881)
                     /locus_tag="OQS60_001925"
     CDS             complement(1883..2881)
                     /locus_tag="OQS60_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2387692.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(2906..4417)
                     /locus_tag="OQS60_001926"
     CDS             complement(2906..4417)
                     /locus_tag="OQS60_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS2387693.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(4440..5423)
                     /locus_tag="OQS60_001927"
     CDS             complement(4440..5423)
                     /locus_tag="OQS60_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS2387694.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(5520..8797)
                     /locus_tag="OQS60_001928"
                     /pseudo
     CDS             complement(5520..8797)
                     /locus_tag="OQS60_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            8915..10108
                     /locus_tag="OQS60_001929"
                     /pseudo
     CDS             8915..10108
                     /locus_tag="OQS60_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(10306..11559)
                     /gene="glyA"
                     /locus_tag="OQS60_001930"
     CDS             complement(10306..11559)
                     /gene="glyA"
                     /locus_tag="OQS60_001930"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS2387695.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            11887..13077
                     /gene="hmpA"
                     /locus_tag="OQS60_001931"
     CDS             11887..13077
                     /gene="hmpA"
                     /locus_tag="OQS60_001931"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS2387696.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13122..13460)
                     /gene="glnB"
                     /locus_tag="OQS60_001932"
     CDS             complement(13122..13460)
                     /gene="glnB"
                     /locus_tag="OQS60_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS2387697.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13521..14855)
                     /gene="glrR"
                     /locus_tag="OQS60_001933"
     CDS             complement(13521..14855)
                     /gene="glrR"
                     /locus_tag="OQS60_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS2387698.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(14845..15558)
                     /gene="qseG"
                     /locus_tag="OQS60_001934"
     CDS             complement(14845..15558)
                     /gene="qseG"
                     /locus_tag="OQS60_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS2387699.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(15723..17150)
                     /gene="qseE"
                     /locus_tag="OQS60_001935"
     CDS             complement(15723..17150)
                     /gene="qseE"
                     /locus_tag="OQS60_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS2387700.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(17708..21595)
                     /gene="purL"
                     /locus_tag="OQS60_001936"
                     /gene_synonym="purI"
     CDS             complement(17708..21595)
                     /gene="purL"
                     /locus_tag="OQS60_001936"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS2387701.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            21853..23409
                     /gene="mltF"
                     /locus_tag="OQS60_001937"
     CDS             21853..23409
                     /gene="mltF"
                     /locus_tag="OQS60_001937"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS2387702.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
BASE COUNT         5557 a         6491 c         5946 g         5426 t
ORIGIN      
        1 atcaaacgca tatcctttat caaacgcagg gtcggaaaca aagatgaaat aacacggtgc
       61 aggtggcgtc gtttccatga tgatggcata accttcctgc ggctgctcga tgcgggcctg
      121 accattccat ccggcaaaac cattgttcac ccactggcgc ggcaacggcg cgagctggtt
      181 aaaatccagt tcctgcggta gctgctcgca aaactcaccc gccagccact gctcccgttc
      241 cagccagtaa ccgctcgcct gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg
      301 atgccagccg gtaccaaatg gcagcgtctc tgccccttga ttggtgacag agagcgtcac
      361 cgtcagcgta tccgccgtta aatgaaacgc ctgacttacc cgatagtgat agacaccgct
      421 gcgatgttca tacaccagac acaaactatc atcgctgtgc gagacacatt gccactcgcc
      481 cagccagcca tcgccgtgta gatagtgcgc atcccactca acattcggtt gcagttgata
      541 ctcacgcccc tgccagacaa aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg
      601 aaaacatgag gcatcggttg ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt
      661 atcgcgccag aagccttcga ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc
      721 gtgggataag gtatagatgg tcatccgcta actccttaat ccgggaagtt aatcacaact
      781 ttcccgcatt tgccgctcgc catcagcgca taggcatcac ctgcctgttc cagcgagaag
      841 cgatgggtaa tggcgttacg cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt
      901 tccatatgga acagactggt cacccaggag ccgataatcc gccgttgatg gtgcatcaga
      961 tcggcgctga cctcgaattc cacttttccg gtttcaccaa tgtaaaccac ccgtccccag
     1021 tcagcggtgg attgcagtgc cagcaagcga cctgcggcat taccagaaca atcgagcgca
     1081 acatccgcgc caccgtgggt gagttcggcg ataatctgcg gcagaccttc ggtggttgct
     1141 aaatagccgt gatccatcac ccctaactgt tttgccatcg ccagacgttc cggcagcata
     1201 tcaacgccga tgatccgttt tgcaccgcga cctttcgcca gcatcatcgc catcatgccg
     1261 accggcccca gaccgaccac cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc
     1321 ccttcatagg ccgtgccaac gccgcaactg ataaacgcac catcttcgta gctcagcgca
     1381 tccggcagga ggatcagatc tttttcttcc gccagcagat attcggcatg accgccgtca
     1441 cgctgccagc cgtaagccgc ttttcctttg ccagtacaag agataggaaa gccgcggcgg
     1501 cagttcgggc aaaaaccaca gccagaaatg tgatacacca gcacgcggtc gccctcttta
     1561 aaatggcggc agccttgccc catcgccaca atctgcccgc acggttcatg accgttgata
     1621 aatccctggt ataacggttt atcgggtgcc gccgccgtgg cacggtgttg atgatagata
     1681 tagtggacat cgcttccgca aatcccggag gatttcattt tgatcagtac ctggttaatc
     1741 cccggcgtcg gcaccgcaac ttcccgcaga tcgacggttg aatttcctgg taaataagct
     1801 gccagcatcg ttttcataaa tccctcatta acaatacgat taattttcat ccctgccgca
     1861 cccgcgccag gggcgttaag gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga
     1921 tattcgccag caccgccacc acgatgatga cgccgcgtac cacctgctgg aaaaaggagt
     1981 taataccgag cagcaccaga ccgttaccga ttagcgtaat caccagcaca ccaagcaatg
     2041 taccgaacag ggaaccgcga ccgccggaaa gtgccgtacc gccgaccacg accgcggcga
     2101 tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca
     2161 gcaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt aaagataaga atgcgcaccc
     2221 gacgaacgtt aataccgcac aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg
     2281 agcgcccgaa ggcggttttt cggctaatga acacaaacag cgcaaacaac accatcatga
     2341 tcagcgcgga tactggcaca ccgagaaatt gtccgcccag ccagtccagc acctcgtttt
     2401 cgtcaatcgg cactggcagt gcgttggtca taaacagccc cattccgcgc agggcgctcc
     2461 acagccccag tgtggcaacg aaacttggta cgttaaacac gccgcgcagc accccggcga
     2521 gcgttcccat cagcgcgcct aacagcaaca ccagcagaca agcaatcgcc aacggaactt
     2581 caaattgcag caaaaatgcc aggcacaccg agacaaaagc caccatcggc ccaacgctga
     2641 catcaatttc accggagata ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg
     2701 cggcatcgcg cagcacgttc atctggttat tcaatgagat aaagccaggc gcgttcaggg
     2761 agaagaccag ataaagaatg gcaatcacca ccagcaaacc gatctcatta atatggcgac
     2821 tgacaaattg tttgagcgag acgctcttgc cctgcggcaa tggtaatgac gaagcagaca
     2881 tcttcaggtt cctcggtaaa attcatcagt gcacagacag aatggcggac atcagctcat
     2941 ccacattgac cggcgagtga aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc
     3001 ggtcacacac cagcggcaac tcctccactt cactggagat aaacaccacg ctttttcctt
     3061 cggcagccag ctcacggaca atacggtaaa tctgctgttt ggcttcgata tcgacgccgc
     3121 gcgttggctc gtcgagcaac aaaatctggc tggcggcata gacccaacga ccgatcacca
     3181 ctttttgctg attgccacca gaaagcgtgc cgatgggcgt ttcgctacta gcggccttga
     3241 ccgtcatccg ctgcatcacc tcttcggtca ggcggcggat ggtggaccat tgcagcactc
     3301 cgttggcgct gattttttgc cgattggtca gcactgtatt ttcgtcaacg cccaaccagg
     3361 gaatgatccc cgcttctttg cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt
     3421 cgccgtaatc ggggcgcgtg attttctcgc cgttgataac aatttcgccc tgttcatacg
     3481 tctccagccc aacaatcgcc ttcagcaatt cactgcgccc tgcccccagc aggccagcaa
     3541 tgccaagcac ttcgccgcga cgtaacgtaa agctgatatc ctccagcttg ggcttatggc
     3601 gtaacgcacg gacttccagt acggcctgat ccacaatttc ctgaggggca accggcgcaa
     3661 tatcaacgtg atcacgccct agcattagcg acacaatatg atgcgtggag gtgttttcga
     3721 gcatcacatc gcccgccacc tgaccatcgc gcataacggt ggcacaggag gcaatgcggc
     3781 ggatttcttc catccggtgg ctgacataaa tcaccgccac gcccagtgcc gacatctttt
     3841 tcaccgcact gatcaccagt tcaacttctg cactggcaag cgagctggtg ggttcatcaa
     3901 gaatgaccac gcgcggctcg cccttcatca cccgtgcaat ttccaccagt tgcttttgcg
     3961 ccgggcttag cgttgaaaca agttgttcag gactaacgtc aacgcccagc gcctgtaagc
     4021 aacgttgggc atcctgcgcc atttgcaggt aatcaatcat gccgttgcgg cgaggccatt
     4081 gaccaaggca gaggttttct gccaccgtca gcccttccac cagacttaat tcctgataaa
     4141 ccgcacgaac ccccagttca gcggcacggc gagtcaacgt agcttcgtca ccttccagtc
     4201 gcgtctcgcc aatccagata tcaccgctat ccgggcgttc gctgccggta agcattcgaa
     4261 tgagagtcga tttgcccgcg ccgtttttac ctaacagcgc acgaacttcg cctttattga
     4321 gcgtgaagtt aacgttatcc agcgcaacga cgccggggta acgcttattt cctgccacca
     4381 cttttgctac cgggactgcc tctgttgccg tgaacatagg gacctctgcg aatcagcgat
     4441 tagggcagac catcaacgtg cgttgccagc cactgtttac catcttccgt tttggtatag
     4501 agatcgattg gcacctgaat cactttttca ccgtcggctt gtttattgat aaccttcaat
     4561 gtttgcgcga aaacagcatt gcccattttc ttaccggaaa tatccactac cgctttcagc
     4621 acctgattgt tttccagctc ctgagcaatt tcggttgtca tatccgaacc gaaaacagca
     4681 atttttccgg cctgattttg attacgtacc gcttttaccg cgccgagtgt cgcaccgcct
     4741 gactccccca taatggcgtt gagatccggc gtggagataa tcagtttttc accaacggaa
     4801 atcgctttat ttaaaacagt cccttcctga ttagcgacaa tttgcgcgcc gggaacgcgg
     4861 gattttaata cttcttcaaa tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa
     4921 ttgatgacgg caattttcgg ctggtcaatt ttattggcaa taaaataatc ggcggcagcg
     4981 ttacccagtt ttttaccaaa ttccagtgga tcgccgacca gatacgccga gatatattta
     5041 tcgacaccct tttgattaat acaggtgttg tagcaaatca ccggaatacc cgcttcactg
     5101 gcgcgacgaa cggtacggct actgccattt tcagacactg ccgataaaat aatggcatcg
     5161 acattacgcg ccacgagggt atcaacaaag gtactttctt tcgaaatatc accctgggcg
     5221 ttagtttcaa ttaactgtac ctgtactgaa gaatcttttg ccgcatcctg aacgccctga
     5281 cgcactccag cgtaatatcc cagggtatca aggtatattg caccaatggt catttcttta
     5341 tccgcagccc tggcaaatag tgcgctacct aatagcgtag ccattaataa tagattacgg
     5401 gttgttctca tttttttagg catagtgcct cctgtagagt ttttattaac aacggcttat
     5461 tctaattatt ttgtgatgag cggcagcgcg tgccgctcaa atattacagt taatagggtt
     5521 tagtgaacgt agttaaaaat aaaaggcatc acggtggtga ataatgccgc tttcggccag
     5581 gccggattaa tcgccgtcaa ttcagcgcga gcagattcat attgcgcaac ctccccgagt
     5641 cccgcggacg ccagcatcgc caccatcagg catttttctt tatgctgctg ttgtaaatcg
     5701 ccatacagcg acaacaggtc aggttgtgaa acagcaaaga aatccgcctc aatactggtt
     5761 ttcgccatct cttgcgccca ctgtttcatt tcactaaaca gttgctgtgc gatttgttgt
     5821 tcgcccagca gtcgcagcgc cattccttgc cagaagagat aatcaaccgg ctgatcgttg
     5881 taataactgt ggatgttaat ggtgcgatcg ccggtcgccg ccagacgtaa acaacgcgtc
     5941 gcttcagttt catcgccctg cgcgttggcg catatcgcct gccagaacca gatgtcgtta
     6001 tcagtttgcc ccggtaaacg gccttcgctt aaattctccg gataatgcag cgcggcatga
     6061 agcagttcgc aggcctgctg cggctgtctg gcatcaagat gctgccaggc gcgtaataac
     6121 tggttgagga taaactgact ggtgaccttc ccttccccgc cttcccacgg gtggaatttg
     6181 cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg tgagatgcca caaattgagc
     6241 agttctgcgg tcatgtcgtc gcgtttcagc gcaatttcca gattattttc cagacgcgcc
     6301 agtcgtttct ccggtgtggc tccacttaac ttatccagca aatcccgttc gaaaagcaga
     6361 cgtgcatcct gcggcgcaag ctgataagca ttatcaagat aacgcgcggc cagttcataa
     6421 tcgtgttgct tattccacgc atggatcgcc aacccgcgcc agccatcggc aaactccggc
     6481 gacatctcta cgcaacgttg ccataaagta atggctttgt tgtagctgcg cttgttgtag
     6541 tagaaacagg ccagtaaatg acgggcaaac cagcactctt caatactctc cagcgccgcc
     6601 acttcttcca gcgtattcgg gaaacggaca aactgcggga agacatcaat ggcttttgcg
     6661 accagttcgc cgcgttcggc tttcggcagc aagctggctt gtagataaag cgataacgtg
     6721 cgctgacagt ccagtgcgtt cagcatctct gtcgccagag tgggcattcc ccagttaatc
     6781 agttgcccgg cggtcatcag ggcgttaacg tcgcgtccct gacacagacc gcgccactgc
     6841 gcgagggctg attcgctacg accatcgaac cagttcagcc accacagaat ggcgttcagc
     6901 ggataatcac gcagcagttt cgcgcgctgc aaacgcgcgt tgtcctgacg accacttaac
     6961 accagcagca gattatgcag gcaaagcact tcctgattgg ttgggcaggc gcgaagactt
     7021 tgttggcaaa aatccagacc agcgtcgaag ttaccattac gcgccgccag tcgtgccagg
     7081 ccataatagc caccggcttt gctgttgccg ctccagaccg cacgccagaa atcctcttcg
     7141 gcttgttgat attgtccctg acgttcgtaa gcactggcgc gaatcaaact tgcttgtcca
     7201 cactgcgggt ttttgttcag cgcatgtgcg cgtttcagcg cctgactggc atacgccacc
     7261 gcttgcggga aatctgcgcg gttatattcc agcatcgcca gcgccaggtt acagcgataa
     7321 tccagcgggt ccagcgccac gccgcgcagg tagtaatcga acggtgaacg gctggcgtga
     7381 tgatattgct ccagatgctg accgataaac caggcttcat ctgtactggt aatgtcttgt
     7441 gctgccagtg gcgctttggc gacgtccggt aacggcaacg cttgcggctg atgttcctga
     7501 taactcagta caatattgcc gtcggcatcg gaaagctcaa tggtcagcct atcaggattg
     7561 ataccgtgca acacgccctg gatggcggtc gcaggcatca gtgccacggc atcatcaagt
     7621 aacgcgttgc atttgccgat ttcgtggatc gccaggcggt atccgttcaa cggagagatg
     7681 gcatacagcc cccactcaat cccctgctca ctacgctgga gttttatcac cgcatcgcgg
     7741 gaggcatttt gcaccatgcc cagagaatga taaggcagga aatactgctc aaaacgcttc
     7801 tcttcgtaag catcaagcca ggtaaaatcg ggctggttat cggcaaaaat accggtcatc
     7861 agttcgatat acgggccgtt attgtcggtc agactcttat cccacgcctg gccaaattca
     7921 ctgtgtcccc aactccactg ttttttacct ggcgcaatat ggtggttggc aacgtgcagc
     7981 aaaccgccat cttcatcgtg acaccacgcg ccgacaaaat cgtactgcga tttttcggcc
     8041 atatatgagg ttggaacggg cacattttta tagcgagaaa tgtccactcc agcggagtag
     8101 tccactttgt agtaagtgcc ggtagcgatg gggaaagcgg agacggcccg tttgccgtga
     8161 tcaaacaccg cagtcaaatc cggtgggaaa acgctctgat gcccttcacc ccctttcact
     8221 gccgggttgg cccaccacaa gaaatgacgc ggcgtggcgt tgccgttata gacgcggctg
     8281 gcgatttcca gcgccgccca gggcgcaggg tgaaacctgt catcacctgt aaaccatgca
     8341 tcggctccgt ttcgcctacc cacaccgtct gtgcaccgtc ttcatgggct tcgagggtga
     8401 aatcaacggg cataaaggtg gtcgggcgat gatgttgcgg ccagttaaac tcaatcccac
     8461 cggaaatcca cggccccagc agccccacca gcgcaggttt aatgacttca ttgtgataaa
     8521 caaaatcgcg ctgtttcact ttatcccacg cgcgatgcac ccgaccgccc agttccggca
     8581 ggatcatcac tttgatgtag tcgttttcca gccacaccgc ctgccaggat ttcagggttt
     8641 tctgctcgct cagcgtatcg gtcacgccgt agggataaac cgcgccggac gatccctgat
     8701 aaacgcgatt ttccaggaac atgggatgta tatcctgcgg cccggtttca taggtcggga
     8761 tctcaacgcg ctcttgccac acttttactg gagtcatggt gccctcaata ttaacaagac
     8821 atactgaatt aaaagatttg tggcagtgta ttgaacaatc tggcaatgtt ttcgcggaat
     8881 aatcacgcaa ttaactaaac aaggtttagt gaagatgaga gcctgcatta acaatcaaca
     8941 gattcgccac cataacaaat gcgtgattct ggaactgctg taccggcaaa agcgcgccaa
     9001 taaatcaacg ctggcccggc tggcgcaaat ttcgattccg gcagtcagta atattttgca
     9061 ggaactggaa agcgaaaaac gggtggtgaa tatcgacgat gaaagccaga cgcgcgggca
     9121 tagtagcggt acatggctga ttgcgccgga aggtgactgg acgctgtgcc tgaacgtgac
     9181 gcccaccagt attgagtgtc aggttgctaa tgcttgttta agtccgaaag gtgaatttga
     9241 gtatttacag attgatgcac cgacgccgca ggcgctgctg tccgaaatcg aaaaatgctg
     9301 gcatcgccac cgtaaattgt ggccggaccg caccatcaat ctggcgctgg caatccacgg
     9361 tcaggttgat ccggtgaccg gcgtgtcgca aaccatgccg caagcgccgt ggacaacgcc
     9421 ggttgaggtg aagtatctgc tggaagagaa gctcggcatt cgggtgatgg tcgataatga
     9481 ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat tcgcagaaac gggatttctg
     9541 cgtgatcaac gttgattacg gcattggctc gtcgttcgtg attaacgagc aaatttatcg
     9601 cggcagtttg tatggtagcg gacagattag tcacaccatc gttaatccgg atggcgtcgt
     9661 ctgcgactgt ggacgttacg gctgcctgga aaccgtcgcc tcgttaagtg cattaaaaaa
     9721 acaggcgcgg gtatggctaa aatcacaacc ggttaatact cagcttgatc cggaaaaact
     9781 gactacagcg cagttaatcg ctgcctggta gagtggagaa ccgtggatca ccagttgggt
     9841 tgatcgcagt gccaatgcca ttggtttgag tctgtataac ttcctcaaca tcctcaatat
     9901 taatcagatt tggttgtacg gtcgcagttg tgcctttggt gagcactggc ttaataccat
     9961 tattcgccag acgggattta acccgttcga ccgcgacgaa gggccgagcg tgaaagcaac
    10021 gcaaattggc tttgggcaat taagccgcgc acaacaggtg ttggggattg gctatttgta
    10081 tgttgaggcg cagttacgac agatttgatg gcgcaataac gtagaaaggc ttcccgaagg
    10141 aagccttgat gatcacatac ctaagattgc ctgatgcgct acgcttatca ggcctacacg
    10201 gatattgcaa tatattgaat ttgcaaagtt ttgtaggccg gataaggcgt tcacgccgca
    10261 tccggcatga acaacgagca cattgacagc aaatcaccgt ttcgcttatg cgtaaaccgg
    10321 gaaacgtgcg cagatgtcga gaactttacc tttgatgcgc tcgataacgg cttcatcatt
    10381 gatgctgtcc agcacgtcac acatccagcc agccagttct ttcgcttctg cttctttgaa
    10441 gccgcgacgc gtaatcgccg gagtacccac gcgaataccg gaggtcacaa acgggctctt
    10501 cggatcgttc ggtacgctgt ttttgttgac ggtgatgtta gcacggccca gagcggcgtc
    10561 tgcttcttta ccggtcaggt ttttatcaac cagatcaacc aggaacaggt ggttatcagt
    10621 gccgccggaa accactttgt agccgcgctc gaggaacact tctaccatcg ctttagcgtt
    10681 tttcgcgacc tgctgctggt aagttttgaa ctcaggctcc atcgcttctt tcagagcaac
    10741 cgctttaccg gcgattacgt gcatcaacgg accgccctga ccaccaggga aaacggcaga
    10801 gttcagtttt ttgtacagct cttcgctacc acctttcgcc aggatcaggc cgccgcgcgg
    10861 acccgccagg gttttgtgag tggtggtagt aacaacgtga gcatgaggaa ccgggttcgg
    10921 gtagacgcca gcagcaacca ggcccgcaac gtgcgccata tcaacgaaca ggtaagcacc
    10981 gatgctgtca gcgatttcac gcattttcgc ccagtcaacc acgccggaat atgcagagaa
    11041 accaccgata atcattttcg gcttgtgttc tttggcttgt ttttccagat cggcgtagtc
    11101 gatatgaccg gtagcatcga taccgtaagg aacgatgttg tacagtttac cggagaagtt
    11161 aaccggagaa ccgtgagtca ggtgaccgcc atgcgccagg ttcataccca gaacggtatc
    11221 acctggttcc agcagcgcgg tgtagaccgc aaagttagcc tgggagccgg agtgcggctg
    11281 gacgttagcg tagtcagcgc cgaacagttc tttcgcacga tcgatcgcca gttgttcaac
    11341 gatatcaaca tactcgcaac cgccgtagta gcgtttgccc ggataacctt cagcatattt
    11401 gttggtcagc tgagaaccct gcgcctgcat tacgcgcggg ctggtgtagt tttcggaggc
    11461 gatcagttcg atgtgctctt cctgacgtac tttttcctgc tccatagcct gccacagttc
    11521 ggcatcataa tcggcaatgt tcatttcacg ctttaacatc cgcatctcct gactcagcta
    11581 acaataaaat ttttggcctt tataggcggt cctgttggac aacggcgaac agtataaccg
    11641 aatcattgtg cgataacagg tcttgacaaa ggaatttacg caaacgatta ccttcaggct
    11701 acgcaaggct ttggagaata aagagcttgc aaccggaaac ggatttcttt tcaggtttgt
    11761 gatgcaaatt tttcacttca tcacattctt tctgaaaaac accaaagaac catttacatt
    11821 gcagggctat tttttataag atgcatttga gatacatcaa ttaagatgca aaaaaaggaa
    11881 gaccatatgc ttgacgctca aaccatcgct acagtaaaag ccaccatccc tttactggtg
    11941 gaaacggggc caaagttaac cgcccatttc tacgaccgta tgtttactca taacccagaa
    12001 ctcaaagaaa tttttaacat gagtaaccag cgtaatggcg atcaacgtga agccctgttt
    12061 aacgctattg ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct gccagcggta
    12121 gaaaaaatcg cgcagaagca caccagcttc cagatcaaac cggaacagta caacatcgtc
    12181 ggtgaacacc tgttggcaac gctggacgaa atgttcagcc cggggcagga agtgctggac
    12241 gcgtggggta aagcctatgg tgtactggct aatgtattta tcaatcgcga ggcggaaatc
    12301 tataacgaaa acgccagcaa agccggtggt tgggaaggta ctcgcgattt ccgcattgtg
    12361 gctaaaacac cgcgcagcgc gcttatcacc agcttcgaac tggagccggt cgacggtggc
    12421 gcagtggcag aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc
    12481 ccgcatcagg aaattcgtca gtactctttg actcgcaaac cggatggcaa aggctatcgt
    12541 attgcggtga aacgcgaaga gggtgggcag gtatccaact ggttgcacaa tcacgccaat
    12601 gttggcgatg tcgtgaaact ggtcgctccg gcaggtgatt tctttatggc tgtcgcagat
    12661 gacacaccag tgacgttaat ctctgccggt gttggtcaaa cgccaatgct ggcaatgctc
    12721 gacacgctgg caaaagcagg ccacacagca caagtgaact ggttccatgc ggcagaaaat
    12781 ggcgatgttc acgcctttgc cgatgaagtt aaggaactgg ggcagtcact gccgcgcttt
    12841 accgcgcaca cctggcatcg tcagccgagc gaagccgatc gcgctaaagg tcagtttgat
    12901 agcgaaggtc tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag
    12961 ttctatctat gcggcccggt tggcttcatg cagtttaccg cgaaacagtt agtggatctg
    13021 ggcgtgaagc aggaaaacat tcattacgaa tgctttggcc cgcataaggt gctgtaattt
    13081 gatgttgccg gatggaaaca tccggcaacc cttgacgcgg tttaaattgc cgcgtcgtcc
    13141 tcttcaccgg tacggatgcg aatgacccgt gccacgtcaa agacgaagat tttaccgtca
    13201 ccgattttgc cggtttgcgc cgtgcgaata atggtatcga cacaggtatc gacaatgtcg
    13261 tccggtacga caatctcaat tttcactttc ggcagaaaat ccaccatata ctccgcgccg
    13321 cggtacagct cggtatggcc tttctggcga ccaaagcctt tcacttcggt caccgtcatg
    13381 ccggtaatac cgacttcggc cagtgcttcg cggacatcgt ccagcttgaa gggttttata
    13441 atcgcatcaa tctttttcat gctattcctt gaaaaggtcg cctgtctttt gatctgctaa
    13501 acgtaacaca taacgccaat tcattccttg aaatcgtttg catccagctc gtgtcgggaa
    13561 agcagtttat aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg ggtgacgttg
    13621 cctttggtga tttgcagcag cttacgcaaa tagttgagtt caaactgatt acgtgcctca
    13681 acaaaggttg gcagcgccgt attttcaccc tccagcgcct gctccaccag cgcatcacta
    13741 atcaccggag atgaggtcag cgccacgcac tgttcaatca cgttgaccaa ctggcgcaca
    13801 ttacccggcc agctcgcggt catcaggcgt ttcatcgcat cggtagagaa cgcgcggaca
    13861 aacggtttat gtcgctctgc cgcctggcgc aacaggtgat ttgccagtag cggaatgtct
    13921 tctgtgcgct ccgccagtgc cggaattttc aggctgacaa cgttgaggcg gtaatagaga
    13981 tcttcgcgga attccccgcg cgccatcgct tttggcagat cacggtgagt ggcagaaata
    14041 atccgcacat tgatatcaat atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc
    14101 aacacgcgca gcagtttgac ctgtaacggc gcaggcatat cgccaatttc atcgagaaat
    14161 agcgttccgc cttccgccgc ctggaataaa ccttcgcgat tgctgacagc gccagtaaac
    14221 gcgccacgcg catgaccaaa cagctccgac tccagcaatt gctcgggcaa tgcgccacag
    14281 ttaatagcaa taaatggttt gctgttgcgc gggctggcgt tgtggatagc ctgggcgaaa
    14341 atctctttcc cggtgccgct ctgaccgtta atcaaaacgc tgacgtctga ttgcgccacc
    14401 agccgcgcct gttccagcaa acgcagcatc agcgggctgc gggtgacaat tgcctcgcgc
    14461 cagcgttcat cggtggctgg cgcggattgc tccagcgcat cgtcaattgc ctgatatagc
    14521 gcatctttat cgacaggctt ggtgaggaaa ctaaaaacgc cctgctgtgt tgcagcaacg
    14581 gcatcgggga tagaaccatg cgcagtaaga ataattactg gcattcccgg ctgcactttc
    14641 tggatttcag caaatagctg cataccgtcc atttcatcca tccgcaggtc gctgataact
    14701 aaatctactt tttcgcgatt cagtacccgt aatccttcag cgccactttc cgccgtgacc
    14761 acactgtagc cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc cggatcgtca
    14821 tcgaccaata ataaatgcgc aggtttatgg ctcatcagga gtgacctcat gggtggatgg
    14881 cgcgggcttt tcactctcgt gcggcgtatc cggcgagaaa tttccagtcg gtttgcgggt
    14941 cgagagctgg cgttcaatat cggtcaggtt ttccagcttg cgggtggtga gttccagttg
    15001 ctgctgtaga acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc tggactgctg
    15061 gagtttactg taacgttggc gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg
    15121 ccagagttga tacagtgggc gaacctgtgc cgggatctcg gtacttaacg cttcaatacg
    15181 cgcgaccagc tggcggcgct catacggcgt aattttggcg tcggcgagca aaatcccttg
    15241 tttaaaggta ttttgccagc tgccgtcgtc atattggcga gcttgctgac gcgactgcgc
    15301 aggcattaaa cgatcagcac aatccatcgc ccgcagccag taaagcggat tggtttcggt
    15361 tgatttacct tgcagcgccc agatgtcgct acattcagta gaaagatagt cagccagttg
    15421 ataaaccgga attttttctt ctgctggcgt atcaatggct ggcttattgt gattctgcac
    15481 gcaacccagc aatgccagac atggcagccc tgccagccac agccgtcggg gcaataatcg
    15541 ttgaaaaatg tgtcgcatat tcaccagact taaagcctat cccagtgggc gtaattgttg
    15601 cagacagtct ggacatggac agcgcggaga aaccggagcg tacatatcgt acgtgaagat
    15661 ttcgagcact gcccggggcc gaactgacaa ataaaatagc ctgataggat aggctgttag
    15721 cattatttcg tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc ttgcccgctc
    15781 tcgtcgacca gatacagttc cccttgcata cggcgaatac aatccctggc aatgcttaat
    15841 cccagaccgc tgcccttcac cgcccctttt cgctggtggc ttccctgaaa aaagggttcg
    15901 aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac atcaatataa
    15961 acccgcgcac catgtgaact gctgcgaagg caaatgttac cggattcagc cccgtagtgc
    16021 accgcattgg agtaaagatt atccagtacg ctcatcagca gcattggctc cgccaggcaa
    16081 gctgttgctt tgagatcgac gtcggtatgc atcattttag cccgtgcggg caggctatga
    16141 gcagaaacca ctgtctccac cagcggtgct aattcaacat tctccagttc caccgcactg
    16201 tccgcctgtt tacggttgta atcaagcagt tgttcgatca gtttttgcaa attgcggctg
    16261 ctgctatcaa gaatgctcac cacctctttt tgctctggcg taagcggccc gacaacctgg
    16321 tcagccagta attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat
    16381 aaatgtctta aaaattgatg gcgttgggat tccagccatg acaggcgctc acttaaccaa
    16441 agaatacgtt gcccaaccga gcgtaactcg ctcggtccac tgaacgaaac gctattgccc
    16501 agagaacgcc cttcccccag acggttgatc atgcgctcga tatttttcac cggcccgata
    16561 atcatccgcg tgaaaagtag caccatcacc agactcacca gaaatagcac cagcgattgc
    16621 caaccaaaat attgcccacg ttcggcgatt tcacgctgaa gttgctgccc acgagagaac
    16681 accactgtgc gcgtggcctg caccatttcg gtattggcac tggcaaaggc ttcaagacgc
    16741 gcggcggcgg cagcatcggg accactgttg ttacactgaa gttgggccag attgttcaaa
    16801 tcctgacgca atgcctggta aagtttatcg tctggcagta cgcctgcgtg ggcatcgagc
    16861 atttcgctgt aacgcttgcg ctggctttga taaaccttcg caagcgtagg gtcgtccagc
    16921 acgcaatact gacggtagct acgctccatc tccagcgccg cgttggtcat cgcttcacta
    16981 cgccgggcat caataagcgt agtgcggtta accagcgccg cctgatcgct aagcgcattc
    17041 aggctttgcc aggcttgcca tgccagcacc aacaggggta gtagaattag caaaaatgcc
    17101 agcattacca gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa ggtgttactc
    17161 tcgtcagacg cgaatagcct gatgctaacc gaggggaagt tcagatacaa caaagccggg
    17221 aattacccgg ctttgttatg gaataaggcg gtgcctaact cgacgtttcg cccgatggtt
    17281 gatatagcta cgctgatatc agaagttgga cggcaggcac cttgttgtgc gtcattcgaa
    17341 ttttatgtag cacgtcccga aggggctgac ataagtcggt gaatgagcca ctgcttacta
    17401 ttatgcagta actgtgccaa taaagaaaat agtttggtaa cgtactgatt atacgttgct
    17461 ttgagggatt tatggcttct tcgctatttg aatgtaaatc agccactgtg tcgctaaaaa
    17521 gagacaactt aagataaact tattagattg caataaaatc aatcatttaa gtgtcgccag
    17581 aaagcgacac ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag ctcccggagt
    17641 tgggagctta tgatagtggt tggtgcttaa tgccgcatcc gggctgcaaa accaatgaac
    17701 tgatgactta ccccaactgc ttacgcgcat tgcggaaaat gcgcatccat gggccatcct
    17761 cgccccagtt ttccggatgc caggagttgc tgacagtacg gaaaacacgt tccgggtgcg
    17821 gcatcataat ggtgactcga ccactttcag tcgtaaccgc cgtaataccg ttcggtgaac
    17881 cgttcgggtt agccgggtag gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg
    17941 ccaccagccc tttgctttcc agtgccgcca gatgcgccgc atcacgcact tccacgcgcc
    18001 cttcaccatg agagacagca atcggcattt gcgagcccac catcccctgc aacagcagag
    18061 acgggctttg ggttacttca accaggctga aacgcgcttc aaagcgatcg gaggtgttac
    18121 gcacaaaacg tggccacaac tcgctccccg ggatcagttc acgcaggtta gacatcatct
    18181 ggcaaccgtt acataccccc agcgccagcg tttgcggacg gtggaagaag gttgcaaact
    18241 catcgcgtac acggtcattg aacaggattg acttcgccca accttcaccg gcacccagca
    18301 catcaccgta ggagaaacca ccgcacgcga ccagggcgtg gaaatcttcc aggcccgtgc
    18361 gtccggtcag caggtcactc atatgcacgt cgatagcatc aaagcctgca cggtggaaag
    18421 ctgccgccat ttcaacatgc gagttcacgc cctgctcacg cagcacagca actttcggac
    18481 gtgcgccagt ggcaatatac ggtgctgcca catcttcgtt gatatcgaac gacagtttca
    18541 cattcaggcc cggatcggcg tcgtttgatt tcgcctggtg ctcctggtca gcacactccg
    18601 ggttgtcacg caggcgctgc atctgccagg tagtttctgc ccaccagaca cgcaacgtgg
    18661 tgcggctttc gctgaataca gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa
    18721 ccgcctgccc tacataatgg acacaatcag caagcccatg ctgtgccagt acggactcga
    18781 ccgcttcacg gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg ttaaacaacg
    18841 ccgccaggcg atcgtcgccc agagaggcaa tatccgcgtt aatgccgcaa tgaccagcaa
    18901 aggccatttc cgccagcgtt accagcaggc cgccatcaga gcggtcgtgg tatgcgagca
    18961 gcttacgctg tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt tgcgcgacgt
    19021 cgcgtacatc tgccggtttg tcgccaagct gacgataaac ctgcgccagc gccgttgcgc
    19081 ccagcgcgtt attgcctttg cccaaatcaa tcagcagcag tgtgttatct tcggtagaaa
    19141 gctgtggcgt gatggtgtga cgcacatctt ccacgcgggc aaatgcagaa atcaccagcg
    19201 acagcggcga cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca
    19261 tagagtcttt acccaccggg atcgtcaggc ccagcgccgg acaaagctct tcgcccacgg
    19321 ctttaacggc ttcatacagg cccgcatctt caccagggtg gcctgccgcc gccatccagt
    19381 tggcggaaag tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata ttggttaacg
    19441 cttcaccgac cgccagacgg gcagaggcgg cgaaatccag cagtgcaacc ggcgcacgct
    19501 cgccaatcgc catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt
    19561 tagcgaccgg cacctgccac ggccccacca tctgatcgcg cgctaccatg ccggttacgc
    19621 tgcggtcgcc gatggtcacc aggaaggttt tctctgccac ggtcggcaga tgcagtacgc
    19681 gtttcaccgc gtctgcaatg gtgatccctt cacggaccag cgcatcgccc ttcgctttca
    19741 gcgtttgtac atcgcgggtc atcttcggcg ttttaccaag caggacgtcc agcggcagat
    19801 cgatcggctg attatcaaaa tgacgatcgt gcagagaaag atgcagttct tcggtcgctt
    19861 caccaatcac cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac agcggtaatt
    19921 gatcggcagc aaccgccagc acgtagcgtt cctgggattc gttacaccag atttccagcg
    19981 ggctcatgcc cggttcgtcg cttagaatat cgcgcagttc aaatttaccg ccgcgcccgc
    20041 cgtcgctcac cagttccggc atcgcgttag aaagaccgcc agcgccgacg tcgtggataa
    20101 acaggattgg gttggcatca ccaagctgcc agcaacggtc gatcacttcc tggcagcgac
    20161 gctccatctc cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca tcagactgac
    20221 ctgacgccat ggaagacgcc gctccgccgc caagaccgat gttcattgcc gggccgccga
    20281 gaacgaccag cttcgcaccg acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt
    20341 tgccgatccc gcccgccagc atgatcggtt tgtgataacc gcgcagctct tcgccgttgt
    20401 ggctgttcac tttttcttca taagtacgga agtagccatt cagtgccgga cgaccaaatt
    20461 cgttgttaaa cgccgcgccg cccagcgggc cttcggtcat gatgtccagc gcggtgacaa
    20521 tgcgctcagg cttaccgaaa tcttcttccc acggctgttc gaagccagga attcgcaggt
    20581 tggaaacgga gaaaccaacc agaccagcct tcggcttcgc accgcgtccg gtggcgcctt
    20641 catcacgaat ttcgccgccg gaacccgtcg ccgcgcccgg ccacggagaa atcgccgtcg
    20701 ggtggttgtg agtttcgact ttcatcagaa tatgcgccgg ttcctgatgg aaatcgtagc
    20761 ggcccgtttc gtggtcagca aagtagcggc ccacttcaga accttccatt acggcggcgt
    20821 tatctttata agcagagaga acgtgatctg gcgtggtttc gaaagtattt ttgatcatct
    20881 tgaacagcga tttcggctgc tgttcaccgt cgataatcca gtcggcatta aaaattttgt
    20941 ggcggcagtg ctcagagttc gcctgagcga acatatacag ctcgatgtcg ttcgggttac
    21001 gaccaagctt tgtgaaagca tcctgcagat agtcaatttc atcttccgcc agagccaggc
    21061 caagacgcag gttagcgtcg atcagcgcct gacggccctg ccccagcaaa tcaacgctgg
    21121 taaccggagt cggttgatgg tgggcaaaca actgctctgc atcatctaaa gcaaaaaaga
    21181 ccgtttccat catgcggtcg tgcagttcag cggtaacctg ctgccattgt tcattggtca
    21241 gcgtaccggc ttctatatag taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc
    21301 cgcagttatg ggcaatatcg gtcgctttcg aagaccaggg agagatggtg ccaggacgcg
    21361 gggtcaccag caggagtttg ccttgcgggg cgtggctggc gagtgccggg ccatatttca
    21421 gcaggcgttc aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg tcagcaaaat
    21481 ggacatactc ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca
    21541 gtttgttgat tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc atcataagtc
    21601 tctcgtcttc taagctttcg gtgtacccaa gggggggaaa cgggcgtcat tataaagaat
    21661 ctgatgcgct gacgaaaccg tttgcgtgga aataaaatca ccatcgtgaa ttagcaacgc
    21721 gtgccgccaa tggctgtaat aagttgccat ctggcgcagg tttacgcaaa atgccgctca
    21781 tttatgagta aacctttcac tattattacg ttttttcaag ctgggacgcg cacgacacag
    21841 agaattaact aattgaaaaa attaaagatt aattatctgt tcatcggcat tctggcactg
    21901 ctgctcgcgg tcgctctctg gccatccatt ccctggtttg gtaaagccga caaccgtatc
    21961 gccgccattc aagcgcgggg agagttgcgt gtgagcacca ttcatactcc cctgacttat
    22021 aacgaaatca acgggaaacc ttttggcctg gattacgaac tggcgaaaca gtttgccgat
    22081 tacctcggtg taaaactgaa agtgaccgtg cggcagaata tcagccagct gtttgacgac
    22141 ctcgataatg gtaacgccga cctgctggcg gcaggactgg tctataacag tgagcgggta
    22201 aaaaattatc agcctggccc tacctattat tccgtgtcac aacaactggt ttataaagtg
    22261 agtcagtatc gcccgcgcac actgggcaac ctgacggcgg agcaactcac cgttgcaccg
    22321 ggtcacgtgg tggttaacga tctccaaacc ctgaaagaca caaaattccc ggaattaagc
    22381 tggaaggtag acgacaaaaa aggctctgcg gaattaatgg aagatgtcat cgaaggaaaa
    22441 ctcgattaca ccattgctga ttctgtcgcc atcagcctgt ttcagcgcgt tcacccggag
    22501 ctcgccgtag cgctcgatat caccgatgaa caaccggtga cttggtttag cccgttagat
    22561 ggcgataata ccctttccgc cgccctgctc gacttcttca acgaaatgaa tgaagacggt
    22621 acgctggcac gcattgaaga gaaatacctg gggcatggcg atgattttga ttacgtcgat
    22681 acgcgcacat ttttacgcgc cgtcgatgcg gtactgccgc agttaaagcc cctgtttgag
    22741 aaatacgccg aagaaattga ctggcgtttg ctggccgcta ttgcttatca ggaatcgcac
    22801 tgggatgcac aggccacttc accgacgggt gtgcgcggca tgatgatgtt aaccaaaaat
    22861 accgcgcaaa gcctcggcat tacggatcgt accgatgccg aacagagcat cagcggtggc
    22921 gtgcgttatt tgcaggatat gatgagtaaa gtgccggaaa gtgtgccgga gaacgagcgg
    22981 atctggtttg ccctcgctgc gtacaatatg ggctatgcgc atatgctgga tgcccgcgcc
    23041 ctgacggcaa aaaccaaagg gaatcctgac agttgggctg acgtaaaaca gtgtctgcct
    23101 ttacttagcc agaaacccta ttacagcaag ctgacttacg gctacgctcg tggacatgaa
    23161 gcctacgctt atgtcgaaaa tattcgtaag tatcagatta gcctggtggg ttatctgcaa
    23221 gagaaagaga agcaggctac agaagcggcg atgcaactgg cgcaggatta tccggcggta
    23281 tcgcctacgg agttgggcaa agagaaattt ccttttctct cgtttctttc ccagtcgtca
    23341 tcaaactatt tgacccattc tccctctctg ctgttttcca ggaaagggag tgaagagaaa
    23401 caaaattaat ccgtcgaggg
//
LOCUS       DAJVYM010000053        23121 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVYM010000053 DAJVYM010000000
VERSION     DAJVYM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.053"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OQS60_001938"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OQS60_001938"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS2387703.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OQS60_001939"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OQS60_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS2387704.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OQS60_001940"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OQS60_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS2387705.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OQS60_001941"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OQS60_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS2387706.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OQS60_001942"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OQS60_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS2387707.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OQS60_001943"
     CDS             5281..5739
                     /locus_tag="OQS60_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS2387708.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OQS60_001944"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OQS60_001944"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS2387709.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OQS60_001945"
     tRNA            6791..6875
                     /locus_tag="OQS60_001945"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="OQS60_001946"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="OQS60_001946"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OQS60_001947"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OQS60_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS2387710.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OQS60_001948"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OQS60_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS2387711.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OQS60_001949"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OQS60_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS2387712.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="OQS60_001950"
     CDS             complement(10157..13900)
                     /locus_tag="OQS60_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS2387713.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OQS60_001951"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OQS60_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS2387714.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="OQS60_001952"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="OQS60_001952"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS2387715.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="OQS60_001953"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="OQS60_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS2387716.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OQS60_001954"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OQS60_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS2387717.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="OQS60_001955"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="OQS60_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS2387718.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OQS60_001956"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OQS60_001956"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS2387719.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OQS60_001957"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OQS60_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS2387720.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="OQS60_001958"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="OQS60_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS2387721.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5795 a         6052 c         5556 g         5718 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcgac cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg caatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg a
//
LOCUS       DAJVYM010000054        23003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVYM010000054 DAJVYM010000000
VERSION     DAJVYM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.054"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="OQS60_001959"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="OQS60_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS2387722.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="OQS60_001960"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="OQS60_001960"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS2387723.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="OQS60_001961"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="OQS60_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS2387724.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="OQS60_001962"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="OQS60_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS2387725.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="OQS60_001963"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="OQS60_001963"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS2387726.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="OQS60_001964"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="OQS60_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS2387727.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11312
                     /gene="ftsK"
                     /locus_tag="OQS60_001965"
     CDS             7245..11312
                     /gene="ftsK"
                     /locus_tag="OQS60_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS2387728.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11467..12078
                     /gene="lolA"
                     /locus_tag="OQS60_001966"
     CDS             11467..12078
                     /gene="lolA"
                     /locus_tag="OQS60_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS2387729.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12089..13432
                     /gene="rarA"
                     /locus_tag="OQS60_001967"
                     /gene_synonym="mgsA"
     CDS             12089..13432
                     /gene="rarA"
                     /locus_tag="OQS60_001967"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS2387730.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13523..14815
                     /gene="serS"
                     /locus_tag="OQS60_001968"
     CDS             13523..14815
                     /gene="serS"
                     /locus_tag="OQS60_001968"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS2387731.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15054..17498
                     /gene="dmsA"
                     /locus_tag="OQS60_001969"
     CDS             15054..17498
                     /gene="dmsA"
                     /locus_tag="OQS60_001969"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCS2387732.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17509..18126
                     /gene="dmsB"
                     /locus_tag="OQS60_001970"
     CDS             17509..18126
                     /gene="dmsB"
                     /locus_tag="OQS60_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS2387733.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18128..18991
                     /gene="dmsC"
                     /locus_tag="OQS60_001971"
     CDS             18128..18991
                     /gene="dmsC"
                     /locus_tag="OQS60_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS2387734.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19027..19653)
                     /locus_tag="OQS60_001972"
     CDS             complement(19027..19653)
                     /locus_tag="OQS60_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2387735.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19968..21116
                     /locus_tag="OQS60_001973"
     CDS             19968..21116
                     /locus_tag="OQS60_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2387736.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21326..22756
                     /locus_tag="OQS60_001974"
                     /pseudo
     CDS             21326..22756
                     /locus_tag="OQS60_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(22757..>23003)
                     /locus_tag="OQS60_001975"
     CDS             complement(22757..>23003)
                     /locus_tag="OQS60_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2387737.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         5650 a         5726 c         6085 g         5542 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cataaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc atttttgtgg tgaaaacaag catcgtggca
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgactg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcatgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca accttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg gggcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccaga gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcgtcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgac
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcacccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 tgaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccgcc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt caggggcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccttg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc ggcacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
     8581 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
     8641 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
     8701 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccac
     8761 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
     8821 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
     8881 caccttctgt tgcagcagta cctccagtag aaaccgctgc cgctgtttct ccgctggcat
     8941 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
     9001 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
     9061 tgccacgacc gaaacgtatc cgcgtgccaa ctcgccgtga attggcgtct tacggtatta
     9121 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
     9181 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
     9241 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
     9301 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
     9361 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
     9421 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
     9481 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
     9541 agcagccaca acagccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
     9661 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcgc
     9721 cgcagcagca gtatcagcag ccacaacagc cagttgcgcc acaaccgcag gataccctgc
     9781 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
     9841 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
     9901 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
     9961 atgttgtcaa ttactctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    10021 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    10081 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    10141 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    10201 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    10261 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    10321 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    10381 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    10441 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    10501 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    10561 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    10621 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    10681 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    10741 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    10801 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    10861 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    10921 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    10981 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    11041 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    11101 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    11161 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    11221 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    11281 gtgaagtgct ggctccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    11341 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    11401 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    11461 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    11521 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    11581 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    11641 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    11701 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    11761 gcaacttggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    11821 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    11881 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    11941 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    12001 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    12061 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    12121 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    12181 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca
    12241 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    12301 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    12361 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    12421 tgttgacgaa gtgcaccgat tcaacaaaag ccagcaggat gcatttctgc cacatattga
    12481 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    12541 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgggtacag aggatattga
    12601 gcaagtacta actcaggcga tggaagacaa gacccgtggt tatggtggtc aggatattgt
    12661 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    12721 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
    12781 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
    12841 aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg gtagcgcacc
    12901 cgatgcggcg ctctactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    12961 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    13021 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    13081 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    13141 tgcgtttaaa gctgcgctgg cggatgctcg cgaacgcccg gattatgacg tgccggttca
    13201 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    13261 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    13321 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    13381 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    13441 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    13501 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    13561 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    13621 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    13681 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    13741 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    13801 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    13861 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    13921 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    13981 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    14041 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    14101 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    14161 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    14221 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    14281 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    14341 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    14401 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    14461 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    14521 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    14581 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    14641 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    14701 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    14761 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    14821 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    14881 caataattac tcttcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    14941 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    15001 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    15061 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    15121 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    15181 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    15241 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    15301 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    15361 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    15421 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    15481 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    15541 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    15601 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    15661 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    15721 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    15781 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    15841 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    15901 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    15961 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    16021 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    16081 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    16141 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    16201 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    16261 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    16321 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    16381 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    16441 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    16501 gtaactgcct ggttaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    16561 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    16621 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    16681 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    16741 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    16801 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    16861 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    16921 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    16981 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    17041 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    17101 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    17161 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg
    17221 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    17281 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    17341 caggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    17401 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    17461 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    17521 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    17581 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    17641 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    17701 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    17761 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    17821 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    17881 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    17941 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    18001 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    18061 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    18121 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    18181 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    18241 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    18301 ctttatcgct tctatgctcc atcttggttc accgatgcgc gcttttaact cgctcaaccg
    18361 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    18421 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctttg
    18481 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    18541 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    18601 gatgttcatg ggcggcccgt tactggggta cctgttgctg agtctggctg gcgtcgatag
    18661 ctgggcgatg cgtctgctgc cagccatttc cgtactggca ctggtggtga gtggcgtggt
    18721 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    18781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    18841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    18901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    18961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    19021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    19081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    19141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    19201 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaacacatc
    19261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    19321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    19381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    19441 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    19501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    19561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cagcagcatc
    19621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    19681 acgcctgaaa tcggtcagac gtgatgcatt gctctgaaag catagacggg aaatatgagt
    19741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    19801 aaaaacaatc agatgagatt aatcatctct tcagtattag cgttatgaat gagtaatata
    19861 aatcgataat accgccgttg cgatatgcgc gtattttcac ccattgacaa tgtttttggc
    19921 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
    19981 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
    20041 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
    20101 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
    20161 ttaaacgcat tggttttaac cgcagctatt atctggcctc cttcattttt gccgctggct
    20221 gtgccggcct tggcctgatg attggattct ggagttggtt ggcgtggcgt tttgtcgcgg
    20281 gcgtcggctg tgccatgatt tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt
    20341 cacgtaaccg tgggcgtttg cttgctgcat acatgatggt ttattacgtg ggaacgtttt
    20401 taggccagtt actggtcagc aaggtctcaa ccgagctgat gagcgtattg ccgtgggtta
    20461 caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
    20521 ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag gcgcggcttg
    20581 gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc ctgatgcctc
    20641 tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg gcggtactgg
    20701 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
    20761 tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg atgcttagcc
    20821 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
    20881 tggcgatggc atgggcttgc gagaaagttg aacatcatca actggtggcg atgaaccaag
    20941 ccttactgtt gagctatact gtgggaagtc tgcttggccc gtcatttacc gctatgctaa
    21001 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
    21061 tgctgatgct gctgcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
    21121 attcaagcag agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
    21181 ttgctttatt ccaaatttta ttcgtttaaa aataaaatgt gtagcaggtt ataattttgc
    21241 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
    21301 tataattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agctgcgatg
    21361 gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa
    21421 ctacgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg cgctctattt
    21481 cacaccttat gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg
    21541 ggtcagtacc tggattaaac acacgatggg acccggactg gcttatctcg ccgcgtggac
    21601 ctactgggtg gtgcatattc cctatctggc acaaaaaccc taggcaattc tgattgcgct
    21661 cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca
    21721 ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc
    21781 gctgaaaatc gtcggttctg tggcagggat tgctatgttt gttatgtcgc tcctgtatgt
    21841 ggcgatggcg gtaaccgcgc ctgcaattac tgaagtgcat attgcgacca caaacattac
    21901 ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt caatgctggt
    21961 tttcgcggtt ggcggagcag agaagatttc tccttacgtt aatcaaacgc gcaacccagg
    22021 aaaagaattt ccaaaaggga tgttatgcct ggcggtgatg gttgcggttt gtgccattct
    22081 gggctcgctg gcgatgggga tgatgtttga ttcgcgtaat atcccggatg acttaatgac
    22141 caacggtcag tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt
    22201 aatggtgatt tacgccattg cgaataccct gggacaagta gcggcgctgg tattctcgat
    22261 tgatgccccg cttaaagtgc tattaggtga tgctgacagc aaatatattc cagccagttt
    22321 atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtactggt
    22381 ggcgattctg attatgctgc cgactctcgg cattggtgat atgaacaatc tctataaatg
    22441 gctgttgaac cttaattcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt
    22501 tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta ttcgtaataa
    22561 gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctttg cctgtttgac
    22621 ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa
    22681 tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc tggcgcgtaa
    22741 agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt
    22801 ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt gggtaataga
    22861 tctggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa cgaccggtat
    22921 caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc atcaaaacgg
    22981 catccagttc agcatcaaca tca
//
LOCUS       DAJVYM010000055        22887 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVYM010000055 DAJVYM010000000
VERSION     DAJVYM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.055"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..524)
                     /locus_tag="OQS60_001976"
     CDS             complement(<1..524)
                     /locus_tag="OQS60_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2387738.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="OQS60_001977"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="OQS60_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS2387739.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1941..2603
                     /locus_tag="OQS60_001978"
     CDS             1941..2603
                     /locus_tag="OQS60_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2387740.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="OQS60_001979"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="OQS60_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS2387741.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /locus_tag="OQS60_001980"
     CDS             3137..3505
                     /locus_tag="OQS60_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS2387742.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="OQS60_001981"
     CDS             3543..3848
                     /locus_tag="OQS60_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS2387743.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="OQS60_001982"
     CDS             3851..4255
                     /locus_tag="OQS60_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS2387744.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="OQS60_001983"
     CDS             4245..4544
                     /locus_tag="OQS60_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS2387745.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4730..5122
                     /locus_tag="OQS60_001984"
     CDS             4730..5122
                     /locus_tag="OQS60_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS2387746.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5192..6205
                     /gene="yqjG"
                     /locus_tag="OQS60_001985"
     CDS             5192..6205
                     /gene="yqjG"
                     /locus_tag="OQS60_001985"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS2387747.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6470..6835
                     /locus_tag="OQS60_001986"
     CDS             6470..6835
                     /locus_tag="OQS60_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS2387748.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="OQS60_001987"
     CDS             7077..7433
                     /locus_tag="OQS60_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS2387749.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="OQS60_001988"
                     /pseudo
     CDS             complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="OQS60_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8485..9186
                     /locus_tag="OQS60_001989"
     CDS             8485..9186
                     /locus_tag="OQS60_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS2387750.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /locus_tag="OQS60_001990"
     CDS             9209..9373
                     /locus_tag="OQS60_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387751.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /locus_tag="OQS60_001991"
     CDS             complement(9507..10817)
                     /locus_tag="OQS60_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS2387752.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="OQS60_001992"
     CDS             complement(10845..12176)
                     /locus_tag="OQS60_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS2387753.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="OQS60_001993"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="OQS60_001993"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS2387754.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="OQS60_001994"
     CDS             complement(13887..14276)
                     /locus_tag="OQS60_001994"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS2387755.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="OQS60_001995"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="OQS60_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS2387756.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="OQS60_001996"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="OQS60_001996"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS2387757.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="OQS60_001997"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="OQS60_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS2387758.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="OQS60_001998"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="OQS60_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS2387759.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="OQS60_001999"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="OQS60_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS2387760.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21420..21764
                     /gene="tdcR"
                     /locus_tag="OQS60_002000"
     CDS             21420..21764
                     /gene="tdcR"
                     /locus_tag="OQS60_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS2387761.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            22196..22887
                     /locus_tag="OQS60_002001"
                     /pseudo
     CDS             22196..22887
                     /locus_tag="OQS60_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT         5976 a         5838 c         5381 g         5692 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaagc gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacacgcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaattg
    22141 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
    22201 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
    22261 aagcactgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
    22321 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
    22381 tggcgttgga tgccgggcaa ctgcacgcat tatgggcgtt ggcctcaaca cgattttacg
    22441 tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc
    22501 atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc
    22561 tggctgtttt acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa
    22621 cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata
    22681 tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc
    22741 agcaagcgat atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca
    22801 cggctgggac ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc
    22861 gggcattatc tgaacataaa acactat
//
LOCUS       DAJVYM010000056        22857 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVYM010000056 DAJVYM010000000
VERSION     DAJVYM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.056"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1364
                     /gene="cscA"
                     /locus_tag="OQS60_002002"
     CDS             <1..1364
                     /gene="cscA"
                     /locus_tag="OQS60_002002"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS2387762.1"
                     /translation="YPHFHLAPPAGWMNDPNGLIWFNDRYHAFYQHHPMSEHWGPMHW
                     GHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSAVDDNGVLSLIYTGHVWLDCAGNDD
                     AIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWREADTWWMVVGAKDPGNTG
                     QILLYRGSSLREWTFDRVLAHADAGESYMWECPDFFSLGDQHYLMFSPQGMNAEGYSY
                     RNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFLAKDGRRIVIGWMDMWES
                     PMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQHQSVSPRTISNKYVLQEN
                     AQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSERLVLWRYYPHENLDGYRSIPL
                     PQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELSLYASHGVAVLQHGALW
                     LLG"
     gene            complement(1372..2430)
                     /locus_tag="OQS60_002003"
     CDS             complement(1372..2430)
                     /locus_tag="OQS60_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2387763.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2598..2816
                     /locus_tag="OQS60_002004"
                     /pseudo
     CDS             2598..2816
                     /locus_tag="OQS60_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2834..4162
                     /gene="dsdA"
                     /locus_tag="OQS60_002005"
     CDS             2834..4162
                     /gene="dsdA"
                     /locus_tag="OQS60_002005"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS2387764.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4270..5808)
                     /gene="emrY"
                     /locus_tag="OQS60_002006"
     CDS             complement(4270..5808)
                     /gene="emrY"
                     /locus_tag="OQS60_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS2387765.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5808..6971)
                     /gene="emrK"
                     /locus_tag="OQS60_002007"
     CDS             complement(5808..6971)
                     /gene="emrK"
                     /locus_tag="OQS60_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS2387766.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7387..8001
                     /gene="evgA"
                     /locus_tag="OQS60_002008"
     CDS             7387..8001
                     /gene="evgA"
                     /locus_tag="OQS60_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS2387767.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8006..11599
                     /gene="evgS"
                     /locus_tag="OQS60_002009"
     CDS             8006..11599
                     /gene="evgS"
                     /locus_tag="OQS60_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS2387768.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11655..12800)
                     /gene="yfdE"
                     /locus_tag="OQS60_002010"
     CDS             complement(11655..12800)
                     /gene="yfdE"
                     /locus_tag="OQS60_002010"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS2387769.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12874..13818)
                     /gene="yfdV"
                     /locus_tag="OQS60_002011"
     CDS             complement(12874..13818)
                     /gene="yfdV"
                     /locus_tag="OQS60_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS2387770.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13888..15582)
                     /gene="oxc"
                     /locus_tag="OQS60_002012"
     CDS             complement(13888..15582)
                     /gene="oxc"
                     /locus_tag="OQS60_002012"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS2387771.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15636..16886)
                     /gene="frc"
                     /locus_tag="OQS60_002013"
     CDS             complement(15636..16886)
                     /gene="frc"
                     /locus_tag="OQS60_002013"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS2387772.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17399..18022)
                     /locus_tag="OQS60_002014"
     CDS             complement(17399..18022)
                     /locus_tag="OQS60_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS2387773.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18319..18594
                     /gene="ypdI"
                     /locus_tag="OQS60_002015"
     CDS             18319..18594
                     /gene="ypdI"
                     /locus_tag="OQS60_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCS2387774.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18671..18913)
                     /locus_tag="OQS60_002016"
     CDS             complement(18671..18913)
                     /locus_tag="OQS60_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS2387775.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19266..20186
                     /gene="lpxP"
                     /locus_tag="OQS60_002017"
     CDS             19266..20186
                     /gene="lpxP"
                     /locus_tag="OQS60_002017"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS2387776.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20542..20613
                     /gene="ypdK"
                     /locus_tag="OQS60_002018"
     CDS             20542..20613
                     /gene="ypdK"
                     /locus_tag="OQS60_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS2387777.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20678..21916)
                     /gene="alaC"
                     /locus_tag="OQS60_002019"
     CDS             complement(20678..21916)
                     /gene="alaC"
                     /locus_tag="OQS60_002019"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS2387778.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22293..>22857
                     /gene="ypdA"
                     /locus_tag="OQS60_002020"
     CDS             22293..>22857
                     /gene="ypdA"
                     /locus_tag="OQS60_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS2387779.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6385 a         5361 c         4997 g         6114 t
ORIGIN      
        1 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
       61 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      121 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      181 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      241 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      301 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      361 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      421 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      481 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      541 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      601 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      661 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
      721 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
      781 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
      841 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
      901 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
      961 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1021 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1081 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1141 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1201 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1261 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1321 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1381 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1441 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1501 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1561 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1621 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1681 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     1741 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     1801 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     1861 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     1921 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     1981 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2041 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2101 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2161 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2221 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2281 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2341 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2401 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2461 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2521 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2581 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2641 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     2701 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     2761 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     2821 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     2881 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     2941 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3001 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3061 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3121 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3181 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3241 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3301 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3361 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3421 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3481 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3541 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3601 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3661 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     3721 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     3781 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     3841 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     3901 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     3961 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4021 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4081 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4141 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4201 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4261 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4321 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4381 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4441 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4501 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4561 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4621 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4681 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     4741 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     4801 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     4861 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     4921 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     4981 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5041 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5101 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5161 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5221 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5281 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5341 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5401 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5461 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5521 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5581 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5641 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     5701 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     5761 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     5821 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     5881 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     5941 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6001 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6061 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6121 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6181 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6241 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6301 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6361 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6421 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6481 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6541 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6601 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6661 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     6721 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     6781 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     6841 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     6901 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     6961 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7021 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7081 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7141 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7201 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7261 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7321 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7381 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7441 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7501 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7561 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7621 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7681 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     7741 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     7801 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     7861 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     7921 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     7981 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8041 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8101 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8161 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8221 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8281 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8341 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8401 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8461 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8521 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8581 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8641 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     8701 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     8761 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     8821 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     8881 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     8941 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9001 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9061 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9121 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9181 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9241 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9301 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9361 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9421 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9481 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9541 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9601 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9661 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
     9721 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
     9781 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
     9841 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
     9901 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
     9961 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10021 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10081 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10141 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10201 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10261 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10321 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10381 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10441 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10501 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10561 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10621 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10681 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    10741 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    10801 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    10861 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    10921 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    10981 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11041 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11101 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11161 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11221 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11281 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11341 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11401 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11461 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11521 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11581 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11641 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    11701 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    11761 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    11821 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    11881 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    11941 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12001 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12061 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12121 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12181 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12241 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12301 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12361 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12421 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12481 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12541 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12601 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12661 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    12721 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    12781 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    12841 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    12901 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    12961 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13021 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13081 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13141 gaactcgaat ttatgtgctg ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13201 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13261 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13321 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13381 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13441 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13501 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13561 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13621 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13681 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    13741 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    13801 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    13861 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    13921 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    13981 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14041 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14101 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14161 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14221 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14281 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14341 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14401 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14461 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14521 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14581 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14641 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    14701 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    14761 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    14821 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    14881 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    14941 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15001 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15061 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15121 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15181 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15241 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15301 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15361 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15421 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15481 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15541 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15601 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15661 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    15721 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    15781 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    15841 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    15901 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    15961 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16021 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16081 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16141 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16201 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16261 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16321 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16381 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16441 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16501 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16561 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16621 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16681 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    16741 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    16801 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    16861 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    16921 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    16981 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17041 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17101 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17161 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17221 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17281 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17341 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17401 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17461 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17521 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17581 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17641 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    17701 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    17761 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    17821 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    17881 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    17941 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18001 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18061 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18121 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18181 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18241 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18301 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18361 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18421 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18481 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18541 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18601 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18661 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    18721 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    18781 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    18841 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    18901 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    18961 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19021 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19081 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19141 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19201 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19261 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19321 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19381 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19441 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19501 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19561 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19621 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19681 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    19741 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    19801 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    19861 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    19921 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    19981 taacgatggt aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg
    20041 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20101 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20161 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20221 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20281 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20341 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20401 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20461 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20521 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20581 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20641 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    20701 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    20761 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    20821 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    20881 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    20941 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21001 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21061 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21121 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21181 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21241 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21301 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21361 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21421 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21481 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21541 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21601 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21661 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    21721 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    21781 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    21841 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    21901 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    21961 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22021 agaacatttt tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22081 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22141 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22201 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22261 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22321 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22381 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22441 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22501 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22561 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22621 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22681 tgaaaatccc caaagcacag cgctggcgcg tcagtattct cggcggcatg ttgtgtgaga
    22741 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    22801 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgc
//
LOCUS       DAJVYM010000057        22677 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVYM010000057 DAJVYM010000000
VERSION     DAJVYM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.057"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="OQS60_002021"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="OQS60_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS2387780.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="OQS60_002022"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="OQS60_002022"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS2387781.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="OQS60_002023"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="OQS60_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS2387782.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="OQS60_002024"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="OQS60_002024"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS2387783.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="OQS60_002025"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="OQS60_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS2387784.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="OQS60_002026"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="OQS60_002026"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS2387785.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OQS60_002027"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OQS60_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS2387786.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="OQS60_002028"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="OQS60_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS2387787.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="OQS60_002029"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="OQS60_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS2387788.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="OQS60_002030"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="OQS60_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS2387789.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="OQS60_002031"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="OQS60_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS2387790.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="OQS60_002032"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="OQS60_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS2387791.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="OQS60_002033"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="OQS60_002033"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS2387792.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="OQS60_002034"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="OQS60_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS2387793.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="OQS60_002035"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="OQS60_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS2387794.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="OQS60_002036"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="OQS60_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS2387795.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="OQS60_002037"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="OQS60_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS2387796.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="OQS60_002038"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="OQS60_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS2387797.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="OQS60_002039"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="OQS60_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS2387798.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="OQS60_002040"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="OQS60_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS2387799.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="OQS60_002041"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="OQS60_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS2387800.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="OQS60_002042"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="OQS60_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS2387801.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OQS60_002043"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OQS60_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS2387802.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..22596
                     /locus_tag="OQS60_002044"
                     /pseudo
     CDS             22225..22596
                     /locus_tag="OQS60_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         5081 a         5688 c         6440 g         5468 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc tgcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat ggcgctagac tggccccctg aatctccaga
    22621 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact agtcatt
//
LOCUS       DAJVYM010000058        22533 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVYM010000058 DAJVYM010000000
VERSION     DAJVYM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.058"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="OQS60_002045"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="OQS60_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCS2387803.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="OQS60_002046"
     CDS             507..1136
                     /locus_tag="OQS60_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCS2387804.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="OQS60_002047"
     CDS             1163..2824
                     /locus_tag="OQS60_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS2387805.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="OQS60_002048"
     CDS             3063..3122
                     /locus_tag="OQS60_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2387806.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="OQS60_002049"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="OQS60_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS2387807.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OQS60_002050"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OQS60_002050"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS2387808.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OQS60_002051"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OQS60_002051"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS2387809.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OQS60_002052"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OQS60_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS2387810.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="OQS60_002053"
     CDS             9505..10125
                     /locus_tag="OQS60_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS2387811.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="OQS60_002054"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="OQS60_002054"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS2387812.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OQS60_002055"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OQS60_002055"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS2387813.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OQS60_002056"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OQS60_002056"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS2387814.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="OQS60_002057"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="OQS60_002057"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS2387815.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OQS60_002058"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OQS60_002058"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="OQS60_002059"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="OQS60_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS2387816.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="OQS60_002060"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="OQS60_002060"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS2387817.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="OQS60_002061"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="OQS60_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS2387818.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OQS60_002062"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OQS60_002062"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS2387819.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="OQS60_002063"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="OQS60_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS2387820.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="OQS60_002064"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="OQS60_002064"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS2387821.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..>22533
                     /locus_tag="OQS60_002065"
     CDS             21328..>22533
                     /locus_tag="OQS60_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this is the primary sigma
                     factor of bacteria; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS2387822.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFL"
BASE COUNT         5481 a         6135 c         5713 g         5204 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc ctt
//
LOCUS       DAJVYM010000059        21997 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVYM010000059 DAJVYM010000000
VERSION     DAJVYM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.059"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OQS60_002066"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OQS60_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS2387823.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OQS60_002067"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OQS60_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS2387824.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OQS60_002068"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OQS60_002068"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS2387825.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="OQS60_002069"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="OQS60_002069"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS2387826.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OQS60_002070"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OQS60_002070"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS2387827.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OQS60_002071"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OQS60_002071"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS2387828.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OQS60_002072"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OQS60_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS2387829.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8253..8468
                     /locus_tag="OQS60_002073"
     CDS             8253..8468
                     /locus_tag="OQS60_002073"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387830.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="OQS60_002074"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="OQS60_002074"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS2387831.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="OQS60_002075"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="OQS60_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS2387832.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="OQS60_002076"
     CDS             11833..12330
                     /locus_tag="OQS60_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS2387833.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="OQS60_002077"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="OQS60_002077"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS2387834.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="OQS60_002078"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="OQS60_002078"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS2387835.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="OQS60_002079"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="OQS60_002079"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS2387836.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="OQS60_002080"
     CDS             15015..15578
                     /locus_tag="OQS60_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS2387837.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="OQS60_002081"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="OQS60_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS2387838.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="OQS60_002082"
     CDS             complement(16169..17149)
                     /locus_tag="OQS60_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS2387839.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="OQS60_002083"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="OQS60_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS2387840.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="OQS60_002084"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="OQS60_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS2387841.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /locus_tag="OQS60_002085"
     CDS             18452..18742
                     /locus_tag="OQS60_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS2387842.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18750..19343
                     /locus_tag="OQS60_002086"
     CDS             18750..19343
                     /locus_tag="OQS60_002086"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS2387843.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="OQS60_002087"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="OQS60_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS2387844.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21769
                     /locus_tag="OQS60_002088"
                     /pseudo
     CDS             20783..21769
                     /locus_tag="OQS60_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            21814..>21997
                     /locus_tag="OQS60_002089"
     CDS             21814..>21997
                     /locus_tag="OQS60_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCS2387845.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT         5443 a         5715 c         5596 g         5243 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga taaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga
    21721 tccaaaacaa gctgctttgt tagagaagtt cgacaacgct gctcaataat attccgggcc
    21781 agtctctggc cctgattttc caagatgctt acaatgatat ttaaccgcct gaaacttgcg
    21841 gtagaccgcg tcattgccgc gttttctgtc gccgtcatgc ttgctctggt tgtatgcgta
    21901 gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac cgatgagctg
    21961 gcacgatttt tgatgatctg ggtagggtta ttaggtg
//
LOCUS       DAJVYM010000060        21643 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVYM010000060 DAJVYM010000000
VERSION     DAJVYM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.060"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQS60_002090"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQS60_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS2387846.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="OQS60_002091"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="OQS60_002091"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS2387847.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQS60_002092"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQS60_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS2387848.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="OQS60_002093"
     CDS             complement(3717..4511)
                     /locus_tag="OQS60_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS2387849.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="OQS60_002094"
     CDS             4649..4990
                     /locus_tag="OQS60_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS2387850.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQS60_002095"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQS60_002095"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS2387851.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="OQS60_002096"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="OQS60_002096"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS2387852.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="OQS60_002097"
     CDS             8906..10198
                     /locus_tag="OQS60_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2387853.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="OQS60_002098"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="OQS60_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS2387854.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="OQS60_002099"
     CDS             complement(10731..11189)
                     /locus_tag="OQS60_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS2387855.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="OQS60_002100"
     CDS             complement(11186..12103)
                     /locus_tag="OQS60_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS2387856.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="OQS60_002101"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="OQS60_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS2387857.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="OQS60_002102"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="OQS60_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS2387858.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQS60_002103"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQS60_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS2387859.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQS60_002104"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQS60_002104"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS2387860.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQS60_002105"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQS60_002105"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS2387861.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="OQS60_002106"
     CDS             16063..16749
                     /locus_tag="OQS60_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2387862.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="OQS60_002107"
     CDS             16746..19160
                     /locus_tag="OQS60_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2387863.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQS60_002108"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQS60_002108"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS2387864.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OQS60_002109"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OQS60_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5016 a         5684 c         5799 g         5144 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat cccccaggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAJVYM010000061        21553 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVYM010000061 DAJVYM010000000
VERSION     DAJVYM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.061"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..1363
                     /gene="espL1"
                     /locus_tag="OQS60_002110"
                     /pseudo
     CDS             1..1363
                     /gene="espL1"
                     /locus_tag="OQS60_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1535..1642
                     /locus_tag="OQS60_002111"
     CDS             1535..1642
                     /locus_tag="OQS60_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387865.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1695..2453)
                     /locus_tag="OQS60_002112"
     CDS             complement(1695..2453)
                     /locus_tag="OQS60_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS2387866.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2740..3669
                     /gene="pfkB"
                     /locus_tag="OQS60_002113"
     CDS             2740..3669
                     /gene="pfkB"
                     /locus_tag="OQS60_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS2387867.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3770..4060
                     /gene="ghoS"
                     /locus_tag="OQS60_002114"
     CDS             3770..4060
                     /gene="ghoS"
                     /locus_tag="OQS60_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS2387868.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4166..5026
                     /locus_tag="OQS60_002115"
     CDS             4166..5026
                     /locus_tag="OQS60_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS2387869.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5067..5603)
                     /locus_tag="OQS60_002116"
     CDS             complement(5067..5603)
                     /locus_tag="OQS60_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS2387870.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5750..6418
                     /gene="hxpB"
                     /locus_tag="OQS60_002117"
     CDS             5750..6418
                     /gene="hxpB"
                     /locus_tag="OQS60_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS2387871.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6581..7171
                     /locus_tag="OQS60_002118"
     CDS             6581..7171
                     /locus_tag="OQS60_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS2387872.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7304..8695
                     /gene="tcyP"
                     /locus_tag="OQS60_002119"
     CDS             7304..8695
                     /gene="tcyP"
                     /locus_tag="OQS60_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCS2387873.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8699..9515)
                     /locus_tag="OQS60_002120"
                     /pseudo
     CDS             complement(8699..9515)
                     /locus_tag="OQS60_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9792..10055)
                     /gene="cedA"
                     /locus_tag="OQS60_002121"
     CDS             complement(9792..10055)
                     /gene="cedA"
                     /locus_tag="OQS60_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS2387874.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10238..12499
                     /gene="katE"
                     /locus_tag="OQS60_002122"
     CDS             10238..12499
                     /gene="katE"
                     /locus_tag="OQS60_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS2387875.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12757..13515)
                     /gene="chbG"
                     /locus_tag="OQS60_002123"
     CDS             complement(12757..13515)
                     /gene="chbG"
                     /locus_tag="OQS60_002123"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS2387876.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13528..14880)
                     /gene="celF"
                     /locus_tag="OQS60_002124"
                     /gene_synonym="chbF"
     CDS             complement(13528..14880)
                     /gene="celF"
                     /locus_tag="OQS60_002124"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS2387877.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14985..15827)
                     /gene="chbR"
                     /locus_tag="OQS60_002125"
     CDS             complement(14985..15827)
                     /gene="chbR"
                     /locus_tag="OQS60_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCS2387878.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15835..16185)
                     /gene="chbA"
                     /locus_tag="OQS60_002126"
     CDS             complement(15835..16185)
                     /gene="chbA"
                     /locus_tag="OQS60_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS2387879.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16236..17594)
                     /gene="chbC"
                     /locus_tag="OQS60_002127"
     CDS             complement(16236..17594)
                     /gene="chbC"
                     /locus_tag="OQS60_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS2387880.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17679..17999)
                     /gene="chbB"
                     /locus_tag="OQS60_002128"
     CDS             complement(17679..17999)
                     /gene="chbB"
                     /locus_tag="OQS60_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS2387881.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18298..18636)
                     /gene="osmE"
                     /locus_tag="OQS60_002129"
     CDS             complement(18298..18636)
                     /gene="osmE"
                     /locus_tag="OQS60_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS2387882.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18838..19665
                     /gene="nadE"
                     /locus_tag="OQS60_002130"
     CDS             18838..19665
                     /gene="nadE"
                     /locus_tag="OQS60_002130"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS2387883.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19895..20782
                     /gene="cho"
                     /locus_tag="OQS60_002131"
     CDS             19895..20782
                     /gene="cho"
                     /locus_tag="OQS60_002131"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS2387884.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20742..21317)
                     /gene="ves"
                     /locus_tag="OQS60_002132"
     CDS             complement(20742..21317)
                     /gene="ves"
                     /locus_tag="OQS60_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS2387885.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5738 a         5314 c         4938 g         5563 t
ORIGIN      
        1 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
       61 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
      121 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
      181 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
      241 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
      301 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
      361 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
      421 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
      481 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
      541 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
      601 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
      661 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
      721 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
      781 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
      841 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
      901 aacaaaaaaa atggtgatga gtattttagc catgaaaata catgatggtg agccaggatt
      961 atacgccgca atggaaaata atcacccttt gtgtgtcaca cggttcctct ctaaaattaa
     1021 tggcatcgcc tttaaataca agttgagcaa agctaacatc atggatttat taaaaggagc
     1081 tacagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg aagacgtcgt
     1141 gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta gtcaacatca
     1201 gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc atatagccat
     1261 tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg tcatcagcca
     1321 aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta tttacgatat
     1381 atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca tgtgcacaac
     1441 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tatccctttt gttacactcc
     1501 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
     1561 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
     1621 ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg cataacacgg
     1681 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
     1741 tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
     1801 aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt atcttccgcg
     1861 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
     1921 ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
     1981 acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca
     2041 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
     2101 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
     2161 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
     2221 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
     2281 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
     2341 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
     2401 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
     2461 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
     2521 attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaataactta gcaatgtatt
     2581 cttatttcat tttttgaata agcatgtggc gaaaacagat ttttatttat atatatttat
     2641 ctgcaaaatt ttaaataaag ctccaataaa tcatattgtt aatttcttca ctttccgctg
     2701 attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat ctatacgttg
     2761 acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc cgaaggaaaa
     2821 ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt cgcccgcgcc
     2881 attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc gaccggcgaa
     2941 cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga agccaaagac
     3001 tggacccggc agaatttaca tgtacatgtg gaagcaagcg gtgagcagta tcgttttgtt
     3061 atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca agttctggaa
     3121 attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt gaagctggaa
     3181 aaattaaccc aactgatttc cgcttcgcaa aaacaaggga tccgctgcat cgtcgacagt
     3241 tctggcgaag cgttaagtgc agcactggca attggtaaca tcgagttggt taagcctaac
     3301 caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccggacga tgtccgcaaa
     3361 gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc ccttggtcca
     3421 caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc accggtgaaa
     3481 agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact gaaactggca
     3541 gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag tgcagccaca
     3601 ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta cgcttacctt
     3661 tcccgctaac aaaaacattc ccccagcatt gggggaatca ccaccaacct gtcggcaacg
     3721 cgtttctccg actttgctca aaagtcatgt gataacaaag gggtgaacta tggccagtgg
     3781 cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga ctgaaattaa
     3841 cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg atgatgaggg
     3901 aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa gtgaagaaga
     3961 aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc ctgaaatcac
     4021 catcacgact tgggaggaat ggaatagcaa cagaaaataa atggtttttg ggcaataatc
     4081 agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa ccttatgatc
     4141 tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt tgagcgagca
     4201 gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag tccatgccgc
     4261 atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa gggaactgct
     4321 tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta aaaccgtcac
     4381 cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg tgatggatta
     4441 tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc aaattgcgcg
     4501 tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact cgctctccac
     4561 aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg aacaacggat
     4621 tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata tcgacgccat
     4681 cgtcgagcat attcagcagc gtctggcctc gcatcaactg cagccttctc tgttgcacgg
     4741 cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt tcgaccctgc
     4801 ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata ctgaacagcc
     4861 gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt tccttgaacg
     4921 tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg gcggtcagca
     4981 tttggttatt gctcagcagt cattggatag attattagca gcatgatatg ggttgaggat
     5041 aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa gaacacataa
     5101 cctgcagcaa tacaaataac cggcaggata tatagtgaga aaaactgcag gaaaatagta
     5161 tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc ttcccctttg
     5221 gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc ctggcgactc
     5281 atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa aatcacccag
     5341 aacataacgt tcacgccacc attaaaatta ggggtcgggg agttatacca gaacagattc
     5401 aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc gagcatgacc
     5461 gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa agaaatcaat
     5521 gtggagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa aacagcaatg
     5581 cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt ttcacttagt
     5641 ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg cgcttaatca
     5701 taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa tgtcaacccc
     5761 gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact cagaaccttt
     5821 acgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct cccgtcgtaa
     5881 cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt ggtacgcccg
     5941 gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg cccgtgccat
     6001 ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagctg ttgcgttatg
     6061 caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata tgctggaaaa
     6121 agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg ctgaaaaact
     6181 gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac tgggcgttga
     6241 cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt ctaaagcagc
     6301 ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac gttttgtatt
     6361 agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc tcggttaatg
     6421 accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt caactgacgg
     6481 attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca ctgtataaaa
     6541 atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg aaggtcacct
     6601 tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc ccgtgctggc
     6661 acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt taccggacat
     6721 cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac cgatcgcccg
     6781 cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc tgctggcaac
     6841 cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac aaggaatggt
     6901 gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg ttcccctgct
     6961 ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg gcaaccaact
     7021 ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc attcattacc
     7081 cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga tccagtttca
     7141 tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt ctggaataag
     7201 caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc caacactatg
     7261 actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact ttccattaat
     7321 tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc gccataaaca
     7381 gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt ttggccttgc
     7441 cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac agtggtttaa
     7501 catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt tagtcttcgc
     7561 ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca aaatcagttt
     7621 tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg gtgtgctggt
     7681 caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag aaactgcacg
     7741 tctgaacgcc attgaaagta actatgttgg taaagtctct gatctgagcg ttccgcagct
     7801 ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca atccgacgtc
     7861 aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga aactgctgaa
     7921 ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac aaagctgggt
     7981 gatgaaactg gttcgccttg tcatgcagtt gaccccttac ggcgttctgg ctctaatgac
     8041 caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt tcgttgtcgc
     8101 gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg gcattaatgg
     8161 cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg cctttaccag
     8221 ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc gtctgggcgt
     8281 tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc agaacggctg
     8341 cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg gcattaaccc
     8401 gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt ccgcaggcgt
     8461 tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg cgatgggcct
     8521 gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca tgggccgtac
     8581 ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt ggctgaagca
     8641 aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc gttaattctt
     8701 atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa tgttccatcg
     8761 cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg tccaacttat
     8821 caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc tcatacccga
     8881 gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca ccacttccca
     8941 ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat attatcgaag
     9001 gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc ggcaggatgt
     9061 atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct attttttata
     9121 ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat cccaacaaaa
     9181 ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat tatcactcta
     9241 tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggagctcc tggctttgag
     9301 gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga ctctgctatt
     9361 tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat ggtagactca
     9421 agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc ataattactc
     9481 tgtattaagt ttgtagaaaa catctcccgc cccatattgt taacaaaatt attatctcat
     9541 ttaaatctaa gtcacttaca atataagttt aagagcgacg ccacaggatg aactatcaaa
     9601 aatagctcat catgattagc aaaacttaac cattttaaaa taaataaaca attaaagaaa
     9661 aaagatcact tatttatagc aatagctcgt caaaggcagc tttttgttac aggtggtttg
     9721 aatgaatgta gcaacgaaat gcagaatttc aggtcatgta actcccggca aaaccgggaa
     9781 gtatgtaatc cttactcagt cacttcccct tcctggcgga tctgatttgc ccaacgttgg
     9841 gcagattcag gcacagtaaa cgccggtgag cgcagaaatg actctcccat cagtacaaac
     9901 gcaacatatt tgccacgcag catccagaca tcacgaaacg aatccatctt tatcgcatgt
     9961 tctggcggcg cgggttccgt gcgtgggaca tagctaataa tctggcggtt ttgctggcgg
    10021 agcggtttct tcattactgg cttcactaaa cgcatattaa aaattagaaa aactgtagtt
    10081 tagccgattt agcccctgta cgtcccgctt tgcgtgtatt tcataacacc gtttccagaa
    10141 tagtctccga agcgggatct ggctggtggt ctatagttag agagtttttt gaccaaaaca
    10201 gcggcccttt cagtaataaa ttaaggagac gagttcaatg tcgcaacata acgaaaagaa
    10261 cctacatcag caccagtcac cactacacga ttccagcgaa gcgaaaccgg ggatggactc
    10321 actggcacct gaggacggat ctcatcgtcc agcggctgaa ccaacaccgc caggtgcaca
    10381 acctaccgcc cctgggagcc tgaaagcccc tgatacacgt aacgaaaaac ttaattctct
    10441 ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg gcgtgcgcat
    10501 cgccgatgat caaaactcac tgcgtgccgg taaccgtggt ccaacgctgc tggaagattt
    10561 tattctgcgc gagaaaatca cccactttga ccatgagcgc atcccggaac gtattgttca
    10621 tgcacgcgga tcagcagctc acggttattt ccagccatat aaaagcttaa gcgatatcac
    10681 caaagcggat ttcctctcag atccgaacaa aatcacccca gtatttgtta ggttctctac
    10741 cgttcagggt ggcgctggct ctgcagatac cgtgcgtgat atccgtggtt ttgccaccaa
    10801 gttctatact gaagagggta tttttgacct cgttggcaat aacacgccaa tcttctttat
    10861 ccaggatgcg cataaattcc ccgattttgt tcatgcggta aaaccagaac cgcactgggc
    10921 aattccacaa gggcaaagtg cccacgatac tttctgggat tatgtttctc tgcaacctga
    10981 aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca gttaccgcac
    11041 catggaaggc ttcggtattc acaccttccg cctgattaat gccgaaggga aggcaacgtt
    11101 tgtacgtttc cactggaaac cactggcagg taaagcctca ctcgtttggg atgaagcaca
    11161 aaaactcacc ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag ccattgaagc
    11221 aggcgatttt ccggaatacg aactgggctt ccagttgatt cctgaagaag atgaattcaa
    11281 gttcgacttc gatcttctcg atccaaccaa actgatcccg gaagaactgg tgcccgttca
    11341 gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac ttctttgccg aaaacgaaca
    11401 agcagcattc catccaggtc atattgttcc tggtctggac ttcaccaacg atccgctgtt
    11461 gcagggacgt ttgttctcct ataccgatac acaaatcagt cgtcttggtg ggccgaattt
    11521 ccatgagatt ccgattaacc gtccgacctg cccttaccac aacttccagc gtgatggcat
    11581 gcatcgtatg gggatcgaca ctaacccggc gaattacgaa ccgaactcga tcaacgataa
    11641 ctggccgcgc gaaacaccgc cgggaccgaa acgcggcggt tttgaatcat atcaggaacg
    11701 cgtggaagga aataaagttc gtgagcgcag tccatcgttt ggcgaatatt attcccatcc
    11761 gcgtctgttc tggctaagtc agacgccatt cgagcagcgc catattgtcg atggtttcag
    11821 ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg accagctggc
    11881 gcatattgat ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg aactgactga
    11941 cgaccagctg aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg atccatcctt
    12001 aagtttgtac gccattcctg acggtgatgt gaaaggtcgc gtggtagcga ttttacttaa
    12061 tgatgaagtg agatcggcag accttctggc cattctcaag gccctgaagg ccaaaggcgt
    12121 tcatgccaaa ctgctctact cccgaatggg tgaagtgact gcggatgacg gtacggtgtt
    12181 gcctatagcc gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg tcattgtccc
    12241 ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc aactactacc tgatggaagc
    12301 ctacaaacac cttaaaccga ttgcgctggc gggagacgcg cgcaagttta aagcaacaat
    12361 caaggtcgct gaccagggtg aagaagggat tgtggaagct gacagcgctg acggtagttt
    12421 tatggatgaa ctgctaacgc tgatggcagc acaccgcgtg tggtcacgca ttcctaagat
    12481 tgacaaaatt cctgcctgat gggaggcggc gcaattgcgc cgcctcaaat gatttacata
    12541 gtgcgctttg tttatgccgg atgcggcgtg aacgccttat ccggcctaca aaactgtgca
    12601 aattcaatat attgcaggaa acacgtaggc ctgataagcg aagcgcatca ggcagttttg
    12661 cgtttgtcag cagtctcaag cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga
    12721 tgcaaggttc acgccgcatc tggcaaacat cctcacttac acatcccgat aactccccaa
    12781 ccgataacca cgctgagcga tagcaccttt caacgacgct gatgtcaaca catccagctc
    12841 cgttaagcgt gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg
    12901 acacatcacc tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa
    12961 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag
    13021 attagccggt aaatcaccag agttagatag cggctgacgg tcgatgcgca acgcaatccc
    13081 ctcttcagcc gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg
    13141 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
    13201 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
    13261 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
    13321 agtaaggaca aagtacatcc ctatggccag actcggttca tcacgactca actgcaccgc
    13381 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
    13441 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
    13501 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
    13561 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
    13621 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
    13681 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
    13741 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
    13801 cttacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
    13861 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
    13921 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
    13981 cgggttttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc
    14041 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata
    14101 aagcaaataa gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag
    14161 atcgaaaata tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa
    14221 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
    14281 gccgaacaaa tcgatagata aatcatcgct gtctttcagc atcagaacat cgtgaataaa
    14341 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
    14401 ataaacggct tcagtaacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
    14461 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
    14521 aaacagaccg cccgcgccgt tggtttcctg gccaagataa ccatgactta atggaatacg
    14581 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
    14641 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
    14701 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
    14761 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
    14821 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
    14881 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
    14941 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
    15001 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
    15061 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
    15121 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
    15181 ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc
    15241 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
    15301 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
    15361 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
    15421 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
    15481 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
    15541 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
    15601 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
    15661 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
    15721 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
    15781 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
    15841 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
    15901 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
    15961 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
    16021 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
    16081 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
    16141 ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc atcatacatc gttcctctct
    16201 attcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc gttagcgata
    16261 tcttcttcgc gctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag
    16321 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg caaccagcaa tgcggcgacg
    16381 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
    16441 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
    16501 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
    16561 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
    16621 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
    16681 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
    16741 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
    16801 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
    16861 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
    16921 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
    16981 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
    17041 cgcgatactg aagcaggtac gctatcgggc aatttaatga cccaattgcg gcggacaata
    17101 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgctcca
    17161 cctaaccagt ttgcgccaac cacataggcc tcaccgacac tatatggggt gacggtcata
    17221 aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc
    17281 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
    17341 ttgccgccaa tacctttcag gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata
    17401 cctaaggaat aaaaaaacgc cccctcccca aagcttagaa aaacgttgtt aattaataca
    17461 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
    17521 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
    17581 ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg cggtattgat
    17641 ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta
    17701 atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag
    17761 tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca
    17821 atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc
    17881 ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat
    17941 accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata
    18001 aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc
    18061 catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg
    18121 gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg
    18181 ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca
    18241 aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta
    18301 cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac
    18361 atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag
    18421 gatgtaggtc tggcaagtac cacgagcatg gatcatgctc acttcagacg aaggtttacc
    18481 cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg
    18541 tacaaactgg tctttagtac gatcataagc cgtacaaccc gccagcatgg ttaataccgc
    18601 cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg
    18661 tgttagataa gcctggaata cattgggcgc tttttcaagc ccgcgaacga aacggctccg
    18721 ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt
    18781 tcagtggcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg
    18841 acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag
    18901 gaaattcgtc gtagtatcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa
    18961 tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag
    19021 atggcgatta atgagctgcg cctggaaacc ggcaacgaat cactgcaatt tattgccgta
    19081 cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat tgcctttatt
    19141 caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggccag cgaacaggca
    19201 ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag
    19261 cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat
    19321 catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg tacagacatt
    19381 aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt tactggcggc attaggttgc
    19441 ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg cccttctctg
    19501 ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa
    19561 aacgtacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa
    19621 cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt tgcacataat
    19681 ggtttgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattcgac
    19741 ctacaaaact atgcaaattc aatatattgc aggagtcgaa taggcctgac aggcgtagca
    19801 cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctc cccgcctgtt
    19861 acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg
    19921 gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt cattccttaa
    19981 tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct
    20041 ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga
    20101 tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgtaccg caggcgaaat
    20161 cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg
    20221 gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt
    20281 gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag
    20341 gcgcgcagct ttgcaagcat tgcagaccat cgctgatgaa caaaaacttt gttatggcct
    20401 gctgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg
    20461 cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc
    20521 tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca
    20581 gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa
    20641 ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta
    20701 taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc
    20761 gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc
    20821 cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag caatttatcg
    20881 cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc aaaggtagta
    20941 aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgtgt catgatgttg
    21001 aaatccatcg acatctgccc ttccgtcagt ttcgctttta ccacctggtc cgctgcaaag
    21061 gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc
    21121 tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga aaactcccca
    21181 ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa
    21241 gtgcaaattt cgcgcgtttc accggcagca tttcgccaca gattcaccga cattttacgc
    21301 atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa
    21361 ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac
    21421 aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag
    21481 tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag ttgcaggcat
    21541 tttacctttt gcc
//
LOCUS       DAJVYM010000062        21191 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVYM010000062 DAJVYM010000000
VERSION     DAJVYM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.062"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..210)
                     /locus_tag="OQS60_002133"
     CDS             complement(<1..210)
                     /locus_tag="OQS60_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCS2387886.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIA"
     gene            complement(207..1768)
                     /gene="casA"
                     /locus_tag="OQS60_002134"
                     /pseudo
     CDS             complement(207..1768)
                     /gene="casA"
                     /locus_tag="OQS60_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1866..4565)
                     /locus_tag="OQS60_002135"
     CDS             complement(1866..4565)
                     /locus_tag="OQS60_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCS2387887.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4760..4912)
                     /locus_tag="OQS60_002136"
     CDS             complement(4760..4912)
                     /locus_tag="OQS60_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS2387888.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5177..5911)
                     /gene="cysH"
                     /locus_tag="OQS60_002137"
     CDS             complement(5177..5911)
                     /gene="cysH"
                     /locus_tag="OQS60_002137"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS2387889.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5986..7698)
                     /gene="cysI"
                     /locus_tag="OQS60_002138"
     CDS             complement(5986..7698)
                     /gene="cysI"
                     /locus_tag="OQS60_002138"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS2387890.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7698..9497)
                     /gene="cysJ"
                     /locus_tag="OQS60_002139"
     CDS             complement(7698..9497)
                     /gene="cysJ"
                     /locus_tag="OQS60_002139"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS2387891.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9816..10178
                     /gene="queD"
                     /locus_tag="OQS60_002140"
     CDS             9816..10178
                     /gene="queD"
                     /locus_tag="OQS60_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS2387892.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10256..11527
                     /locus_tag="OQS60_002141"
     CDS             10256..11527
                     /locus_tag="OQS60_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2387893.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11518..11778
                     /locus_tag="OQS60_002142"
     CDS             11518..11778
                     /locus_tag="OQS60_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS2387894.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11795..12370
                     /locus_tag="OQS60_002143"
     CDS             11795..12370
                     /locus_tag="OQS60_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS2387895.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12518..13378)
                     /locus_tag="OQS60_002144"
     CDS             complement(12518..13378)
                     /locus_tag="OQS60_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS2387896.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13375..14154)
                     /locus_tag="OQS60_002145"
     CDS             complement(13375..14154)
                     /locus_tag="OQS60_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS2387897.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14132..15541)
                     /locus_tag="OQS60_002146"
     CDS             complement(14132..15541)
                     /locus_tag="OQS60_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2387898.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15563..17017)
                     /locus_tag="OQS60_002147"
     CDS             complement(15563..17017)
                     /locus_tag="OQS60_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS2387899.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17087..17903)
                     /locus_tag="OQS60_002148"
                     /pseudo
     CDS             complement(17087..17903)
                     /locus_tag="OQS60_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            18221..19498
                     /locus_tag="OQS60_002149"
     CDS             18221..19498
                     /locus_tag="OQS60_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2387900.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19525..21010
                     /locus_tag="OQS60_002150"
                     /pseudo
     CDS             19525..21010
                     /locus_tag="OQS60_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5098 a         5613 c         5220 g         5260 t
ORIGIN      
        1 ggctatcagc gccagtgcgg tgatacgcca ctcaggttca tcctgccact ttatttgtct
       61 gttcttcaga aacaggcgaa atccatccgt cagacaaaca tcattaaccg tcgtactacg
      121 ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt tgcgcagcgt
      181 ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt ctccctggcg
      241 gctttagcac tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc ggtcatgatg
      301 cgttccaaat caacgggctc ataaggattg gtgaatacac gctcgtcaaa atcctgacgc
      361 gcgaataacc aaatttcctt attccatttg ctgagtaatt catccgcatc ctgaccttct
      421 tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa gtcgatatcc
      481 acaaaactaa aatcacccct tgcacctttt ggatcggaga accatgcttc tttcaatgca
      541 ctccgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag tttcggtatc
      601 tggccttctt tttattgagc agcagcggga aatgatgttc gtaccaacag cgcgctttca
      661 tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg agactgctgg
      721 cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac ttacccgttt
      781 cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg accgagtaaa
      841 actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc cacatggcat
      901 agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc tgctcaccgc
      961 aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg cgaggcattc
     1021 cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg gccagttcgc
     1081 tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg ggtagcggta
     1141 aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag ggagtttgtt
     1201 gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg ccgcgtaaac
     1261 cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac agagctaacg
     1321 cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg tctttattaa
     1381 acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga actttatctc
     1441 cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac tggaaagcat
     1501 gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct tcccagatat
     1561 catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc agcaaaaact
     1621 gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg
     1681 ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg
     1741 gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg
     1801 tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt cctgtcgcag
     1861 acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccaca actttcgtca
     1921 tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat gcaccacagc
     1981 ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg
     2041 cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca
     2101 ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg tgtcgacagt
     2161 ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacaaac
     2221 tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca
     2281 aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag ccgggctgcg
     2341 tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca gattcgccca
     2401 tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc gtcgccagga
     2461 gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc gtcctttccg
     2521 tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga
     2581 atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca atcgacactc
     2641 tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct atcttcttta
     2701 ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta
     2761 tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatgaacc
     2821 ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg acttaccgcc
     2881 gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct gacgctacgc
     2941 tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt gacatgcgtc
     3001 agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc tgtaccccgc
     3061 gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga aagcaaaata
     3121 acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca cgacatgtaa
     3181 gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc
     3241 agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt gccgacgcca
     3301 atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca tccctgactg
     3361 aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag tgattcatta
     3421 aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg ggattctggc
     3481 gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt
     3541 gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct gtgcgccagg
     3601 ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt
     3661 ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata
     3721 aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg
     3781 gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat atccattggg
     3841 tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt
     3901 gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc atcccagaat
     3961 gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca
     4021 gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg ccaaccatga
     4081 tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc
     4141 gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc
     4201 agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat cttgagttca
     4261 gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg ccagcacagc
     4321 aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa
     4381 tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc
     4441 gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcgggttttt
     4501 ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta
     4561 cgcatatatt aaaaattatc tgtgatatat atcattcaat aaaatattta cattatgaat
     4621 aatcaaataa ttttcatccg taatatatat gaataataaa cataggcata atattcacat
     4681 aaataatata aattaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg
     4741 ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca
     4801 taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc gtgaatccca
     4861 gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc atgtcttgcc
     4921 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg
     4981 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
     5041 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
     5101 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta
     5161 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
     5221 gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta tcaccgaccg
     5281 ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat
     5341 aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
     5401 ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg
     5461 tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt
     5521 acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg
     5581 ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     5641 caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat
     5701 ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     5761 aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct tcagcgtcca
     5821 gtttttccag ttcggcgtta gtttccgcca gcgccagaat acgatctacc ttcggcagtt
     5881 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     5941 cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     6001 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     6061 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     6121 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     6181 gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat
     6241 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     6301 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctctgc
     6361 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt tttcacgctg
     6421 cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc
     6481 gctttccggt acaccggtga tgatcagatt ctggttcgcc gtaatgcgga aatcgccttt
     6541 gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat
     6601 gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc
     6661 aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt tgatccccgc
     6721 gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt
     6781 tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc
     6841 ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcgctcgccg tgcgggcgta
     6901 ggttttcttg ttgccgtgtt cgatagaaag cccaccgccc accagcaggt tgaagcctac
     6961 cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc
     7021 gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag
     7081 gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct ccgcatacgc
     7141 gcgggtacga ggcaacagat gctcagaaat cttcttcgcc cactcgtacg cttccgcgtg
     7201 cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagctgt
     7261 cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt
     7321 cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt
     7381 gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg
     7441 cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     7501 acggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttgtc
     7561 gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     7621 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     7681 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca
     7741 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     7801 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     7861 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacataa
     7921 actttttctt tttggtcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg
     7981 acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag
     8041 aacagccagt ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa ggcacggaac
     8101 ggcgcgatgc cggtaccggg gccaatcata atcactggcg tttgcgggtt agcgggcagg
     8161 cggaaattgt cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcgatcc
     8221 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     8281 ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg
     8341 cgcggggtca gcgggcgcag caggttaatc agcgcttcgg catccagttg cgccggagag
     8401 aaacgcacca tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt cgctttatcg
     8461 cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg
     8521 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     8581 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     8641 agtgccgggt cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggcct
     8701 gaatcgccta agtcaatttc gatatgacga acgtcttttt cagagttacg cccggtaatt
     8761 ttctggttaa ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg
     8821 atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     8881 ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacatcg
     8941 gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct gtcgaaatct
     9001 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg
     9061 gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     9121 tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt ttcgctggcg
     9181 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgctgctaat
     9241 aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga ggcggagata
     9301 atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc
     9361 tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtagg cgttaaatcg
     9421 gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt
     9481 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     9541 cagcgcccat aaaacagaag agaagataag gttaacgggg tgaacggtgt ggattaaaga
     9601 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     9661 caaaatagat taaccaaccc aatgaaaaac aaatgaattt agccaatcat taagataaat
     9721 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     9781 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     9841 tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc
     9901 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     9961 gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat gagcgcctcg
    10021 atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa
    10081 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
    10141 aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
    10201 ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
    10261 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
    10321 ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa
    10381 aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc cgcaatttca
    10441 tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgcccga
    10501 tggcgtaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
    10561 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg
    10621 agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga
    10681 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
    10741 acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
    10801 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
    10861 agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac
    10921 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagtcg
    10981 tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat
    11041 caaaaacacg gaatcactgg agtatggtgc gcatctggag ccagaaggtg gcttgcacag
    11101 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
    11161 cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cacaggcggc
    11221 ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt ttgcgcttta
    11281 tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc agcatgttcc
    11341 ggcgcttttg caacgccctg gctggtatcg ggcgtggcct gcgttaatgc aggatatttc
    11401 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggcg
    11461 tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg
    11521 tctgtagccc ataatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc
    11581 gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggcaggtct tttttcgctc
    11641 acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt
    11701 ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc
    11761 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tactccgcca
    11821 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcta tcgattctga
    11881 atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa
    11941 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
    12001 taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac
    12061 caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt
    12121 tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
    12181 ttgtattgag atcctgccag gcggtatgcc caaagtgctg ggctgggtga cagagaaaat
    12241 ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat
    12301 taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa
    12361 attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc
    12421 acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc
    12481 caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt
    12541 ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg
    12601 aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc
    12661 ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca
    12721 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
    12781 gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg
    12841 cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa
    12901 catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca
    12961 gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg
    13021 aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg
    13081 atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat
    13141 gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
    13201 caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca
    13261 ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg
    13321 ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg
    13381 ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg
    13441 cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat
    13501 cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg
    13561 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
    13621 aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa
    13681 cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc
    13741 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
    13801 atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa
    13861 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
    13921 gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
    13981 accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa
    14041 acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt
    14101 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
    14161 ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg
    14221 tcacaacaaa atcaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc
    14281 actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg
    14341 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
    14401 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg
    14461 ttaatgaact gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa
    14521 aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg
    14581 cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa
    14641 tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga
    14701 caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga
    14761 tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc
    14821 gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggtgatt
    14881 ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta
    14941 gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca
    15001 catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg
    15061 gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa
    15121 tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag
    15181 caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga
    15241 tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc
    15301 ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga
    15361 cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt
    15421 gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt
    15481 cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca
    15541 tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa
    15601 tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt
    15661 ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc
    15721 cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt
    15781 cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt
    15841 tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact
    15901 cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg
    15961 acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca
    16021 ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc
    16081 gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa
    16141 tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtctgca aaatgggtga
    16201 agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca
    16261 ccatgacttc acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata tagccgtaga
    16321 agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa
    16381 gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca
    16441 cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca acgaccatat
    16501 cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct accaagccgc
    16561 ccatctgcgc cagcggcctt gactgcggag aatgccccgt ggtgtaacct ttttcacgca
    16621 acgcattttc cagcacctcc agcggaacgc cacattgcgc cgtcgcctgc atattctcaa
    16681 tatcaatatt aatgatttga ttcatggcgg agccgtcaag caccaccgag ttttccacaa
    16741 cggtttccag cccaccttca gtggcggaag cgccggtacg cggcacaccg ttaattttgt
    16801 gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt tttacgaccg
    16861 ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa
    16921 tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct
    16981 ttagttggtc gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa
    17041 aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat
    17101 aaccaccatt gaccactaat aaatgcccat tgacataatt tgaagccgga cttgcgagga
    17161 atacagctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa
    17221 gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat
    17281 agccaggagc aataccattt acctgaatat tatattgacc tagttcatca caataagctt
    17341 tggtgaatcc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta
    17401 agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt
    17461 ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc
    17521 agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca
    17581 gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt
    17641 ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct
    17701 ttgtttcgcc gttatctttc aatcatttcc tttgtttcgc cgttatcttt gacgaaacta
    17761 ggaataaaga tatttgcgcc agctttggcc aacgccatgg caaatgcctg gcctaaaccg
    17821 ctattcccac cggtaacaat tgcggtttta cctttcagtg agaaaaaatc cattgagaac
    17881 gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaagaaag
    17941 gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac tggagataat
    18001 ttttagcata acggcataac tgctaatgtg atcgtaatca cagtgtgata ttcgtattgt
    18061 caaatagttt taaaaagcag aagcgtgatt actctcacac aacacctctg ctttccatgt
    18121 tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa gggcggctta
    18181 cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg
    18241 ccgttggata accctcgcga taattagttt tagcggcggc gttagttttg acctggctta
    18301 tttacgttat atttttcaaa ttcccatggc gaaatttatg ggattcagca ataccgagat
    18361 aggtttaata atgagtacct ttggtattgc ggctattatt ctttatgccc ccagcggcgt
    18421 tattgccgat aaattttcac accgcaaaat gattacttcc gcgatgatca ttaccggatt
    18481 actgggtctg ttaatggcaa cgtatccacc gctgtgggta atgctctgta ttcaggtcgc
    18541 ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg cctcgttgct
    18601 gggcgatcat agtgagcaag ggaaaattat gggctggatg gaagggctgc gcggcgtcgg
    18661 tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac cggatgacag
    18721 cgccagcctg aaaacagtca ttatcatcta cagtgtggtt tacatcttgt tggggattct
    18781 gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg aagaaaaaca
    18841 gtcattccag cttagcgaca tccttgccgt tttgcgtatc agcaccacct ggtattgcag
    18901 catggtgatt tttggtgtct tcacaatcta cgccattctg agttactcca ccaactatct
    18961 gaccgaaatg tacggcatgt cgctggtggc agcgagctac atggggattg tgatcaacaa
    19021 aatcttccgc gcgctgtgcg gcccacttgg aggcattatc accacctaca gtaaagtgaa
    19081 atcccctacc cgcgtgattc aaatcctttc catcatcggt ttgctggcgt tgacagccct
    19141 gctcgtcacg aactctaacc cccaatcggt cgcgatgggg attggcctga ttttactgct
    19201 gggattcacc tgttacgcct cacgcgggct gtactgggcc tgccccggcg aagcgagaac
    19261 accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat tcctgccgga
    19321 tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgctg cagaagccta
    19381 ccgcaatatg tggctgatgg gcatggcggc gctcggcatg gtgattgtct ttaccttttt
    19441 gctgttccaa aaaattcgta ctgctgatag cgcccccgca atagctagca gcaagtaagc
    19501 caggaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat tgatggcgga
    19561 agtcagagca caaaagtggt gatgtacgat ctggaaggta atgtggtttg cgaaggtaaa
    19621 ggcttattac agccgatgca cacgcctgat gccgatactg cagaacatcc tgacgacgat
    19681 ttatgggcat cattatgttt tgcaggtcac gatttgatga gccagtttgc cgggaataaa
    19741 gaagatattg ttggcatcgg cctgggctcc atccgttgct gccgtgcgtt attgaaagcc
    19801 gacggcacgc cagctgcgcc gttgattagc tggcaggatg cacgcgttac acgcccttac
    19861 gaacatacga atcctgacgt ggcgtatgta acctcttttt cgggttatct gacgcatcgc
    19921 ttaaccggcg aatttaaaga taatatcgcc aactattttg gtcagtggcc ggtggattat
    19981 aagacctggg catggagcga agatgccgca gtcatggaga agtttaatat cccccgtcat
    20041 atgctgtttg atgtgcaaat gccaggcact gttcttgggc atatcacacc tcaagccgcg
    20101 ctggcgacgc atttcccggc aggactgccg gttgtttgta ccaccagtga taaaccggta
    20161 gaagctctgg gagccggatt actggatgat gaaactgcgg taatttctct tggcacttac
    20221 atcgcattga tgatgaacgg caaagccttg ccgaaagatc cggtggcgta ctggccgatt
    20281 atgtcttcta ttccgcaaac attgctgtat gaaggttacg gtattcgcaa aggcatgtgg
    20341 acggtgagct ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc cagggcgcag
    20401 gatctttcac cggaagattt actcaacaag aaagcctcta gtgtgccacc tggctgtaat
    20461 gggctgatga cggtgctgga ctggctgacc aatccgtggg aaccgtacaa acgcgggatt
    20521 atgatcggct ttgattccag catggattac gcatggatat atcgttcgat attggaaagc
    20581 gtggcgctga cgctgaagaa caattacgac aatatgtgta atgaaatgaa tcactttgcg
    20641 aaacatgtaa tcattactgg cggcggttcg aacaggatct gtttatgcag atttttgccg
    20701 acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc ctgggggcag
    20761 cgattaatac aggggtaggt ctggggctat acccggatta cgcaacggct gtcgataaaa
    20821 tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga
    20881 tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt
    20941 atgaagtgat gcatggggaa ttggggaatt ggggaatgcg gattcgatcc agagctggtc
    21001 gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg cctgaacata cctgaatggt
    21061 tatccccgct ggcgcgggga acataagttc gacaacttaa aaaatgataa caagtccggt
    21121 ttatccccgc tgatgcaggg aacataataa aaactttaac tagttcggtt tatccctgtt
    21181 tttacaagga a
//
LOCUS       DAJVYM010000063        21149 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVYM010000063 DAJVYM010000000
VERSION     DAJVYM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.063"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(25..570)
                     /gene="hemG"
                     /locus_tag="OQS60_002151"
     CDS             complement(25..570)
                     /gene="hemG"
                     /locus_tag="OQS60_002151"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS2387901.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(582..2033)
                     /gene="trkH"
                     /locus_tag="OQS60_002152"
     CDS             complement(582..2033)
                     /gene="trkH"
                     /locus_tag="OQS60_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS2387902.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2072..2686)
                     /locus_tag="OQS60_002153"
     CDS             complement(2072..2686)
                     /locus_tag="OQS60_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS2387903.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2686..4017)
                     /gene="pepQ"
                     /locus_tag="OQS60_002154"
     CDS             complement(2686..4017)
                     /gene="pepQ"
                     /locus_tag="OQS60_002154"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS2387904.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4207..6396
                     /gene="fadB"
                     /locus_tag="OQS60_002155"
     CDS             4207..6396
                     /gene="fadB"
                     /locus_tag="OQS60_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS2387905.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6406..7569
                     /gene="fadA"
                     /locus_tag="OQS60_002156"
     CDS             6406..7569
                     /gene="fadA"
                     /locus_tag="OQS60_002156"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS2387906.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7759..8460)
                     /gene="fre"
                     /locus_tag="OQS60_002157"
     CDS             complement(7759..8460)
                     /gene="fre"
                     /locus_tag="OQS60_002157"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS2387907.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8507..10000)
                     /gene="ubiD"
                     /locus_tag="OQS60_002158"
     CDS             complement(8507..10000)
                     /gene="ubiD"
                     /locus_tag="OQS60_002158"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS2387908.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10167..10655
                     /gene="rfaH"
                     /locus_tag="OQS60_002159"
     CDS             10167..10655
                     /gene="rfaH"
                     /locus_tag="OQS60_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS2387909.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10652..11434)
                     /gene="tatD"
                     /locus_tag="OQS60_002160"
     CDS             complement(10652..11434)
                     /gene="tatD"
                     /locus_tag="OQS60_002160"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS2387910.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11476..12252)
                     /gene="tatC"
                     /locus_tag="OQS60_002161"
     CDS             complement(11476..12252)
                     /gene="tatC"
                     /locus_tag="OQS60_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS2387911.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12255..12770)
                     /gene="tatB"
                     /locus_tag="OQS60_002162"
     CDS             complement(12255..12770)
                     /gene="tatB"
                     /locus_tag="OQS60_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS2387912.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12774..13043)
                     /gene="tatA"
                     /locus_tag="OQS60_002163"
     CDS             complement(12774..13043)
                     /gene="tatA"
                     /locus_tag="OQS60_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS2387913.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13122..14762)
                     /gene="ubiB"
                     /locus_tag="OQS60_002164"
     CDS             complement(13122..14762)
                     /gene="ubiB"
                     /locus_tag="OQS60_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS2387914.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14759..15364)
                     /gene="ubiJ"
                     /locus_tag="OQS60_002165"
     CDS             complement(14759..15364)
                     /gene="ubiJ"
                     /locus_tag="OQS60_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS2387915.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15378..16133)
                     /gene="ubiE"
                     /locus_tag="OQS60_002166"
     CDS             complement(15378..16133)
                     /gene="ubiE"
                     /locus_tag="OQS60_002166"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS2387916.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16228..17655)
                     /gene="rmuC"
                     /locus_tag="OQS60_002167"
     CDS             complement(16228..17655)
                     /gene="rmuC"
                     /locus_tag="OQS60_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS2387917.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17796..18557)
                     /gene="udp"
                     /locus_tag="OQS60_002168"
     CDS             complement(17796..18557)
                     /gene="udp"
                     /locus_tag="OQS60_002168"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS2387918.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18819..19631
                     /locus_tag="OQS60_002169"
     CDS             18819..19631
                     /locus_tag="OQS60_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS2387919.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20025..21086
                     /gene="hcp"
                     /locus_tag="OQS60_002170"
                     /pseudo
     CDS             20025..21086
                     /gene="hcp"
                     /locus_tag="OQS60_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5200 a         5671 c         5302 g         4976 t
ORIGIN      
        1 ccgcgcattt tttattcttt atgcttattt cagcgtcggt ttgttggtta actgggcgat
       61 ttctcgggcg aaattcgcca cctgctccca atcggtatag acaacttctt tgcgcgtatc
      121 cgtttcaccg cctgacatct tcataattag cttgatcata aaacgatcgt accagcgata
      181 acgtgggtaa cgcagcgccc cggcaatgac cgcgcagcga tcgggacgcc attgcgagtt
      241 catcagaaac ttccgcgcgt agctgttggt ctgtggagta cgcttctccg gtttgcgcgc
      301 caccaggttt acggaataaa aggcgctcgg catcgaattc agtcgcgtcg catgtttttt
      361 gacaaattcc tggaacgcag agtggtagtg accatagcga atagaagcac caatgaccac
      421 acggtcataa ttttcccatt gtggttcttc aatgcggtgc acattggcga catccgtctg
      481 gatccccagt tctttcagtt ccgaagccag gtaggaggca atctcgcgcg tttgtccgtc
      541 ccttgttgag aaaagaatta atgttttcac gtattactcc atcattcacg ccagaaagtc
      601 ggggtaaaga gcaccagcaa tgtaaagacc tcgagacgac caaacagcat gttggcaatc
      661 aggatccatt tagccaccgg gttcatactg gtaaagttat cagcaaccac gccaagccct
      721 ggccccaggt tattcaatgt cgcaacaact gaagcaaagg cagaaaagtc atccacgccc
      781 gtggcgataa tcgccagcat actgacaata aacaccaatg cataggcgga gaaaaatccc
      841 caaacggctt cgaggatacg ttccggcagt gcgcgattcc ccagcttaat gctatagacg
      901 gcgttcggat gcaccagtcg tttcagctca cggttcccct gcttaaacag cagcaggatg
      961 cggatcactt tcaggccacc gcccgttgac ccggcgcaac cgccgataaa agctgaacat
     1021 aaaagcagta ccggcaaaaa gagcggccag cgggcaatgc tgtcagtcgt aaacccggcg
     1081 gttgtcgcca tcgataccac ctggaaaaac gcctggttaa ttgtcatcag cgccgaactg
     1141 tagacattat gaaaccacag tacgagtgta caaataacca ccagcgtaaa ctgtacgccg
     1201 ataaacatgc gaaattccgg atcgcgccaa taaaccttca gactacgccc acttaacagt
     1261 gaaaagtgca gaccgtagtt acagccggag atcagcagga agatagcaat gatagtgtta
     1321 atagtcgggc tgtcgaaata accgatactg gcatcatgtg tcgagaagcc gccaatagcg
     1381 atagtcgcaa agctatggcc gatggcatca aaggcatcca ttccggcaaa ccacaacgcc
     1441 agcgcacagg cgacggtcag caagacataa atcaaccaca gggttttcgc cgtttccgca
     1501 attcgcgggc gcattttgtt atctttcagc gggccgggca tttctgcgcg atagagctgc
     1561 atcccaccca cgccgaggat aggcagtatc gcaaccgcta acacgatgat ccccatcccg
     1621 ccaaaccatt gcagcatctg gcgataaaag aggatggcat gagggagcga atccagcccc
     1681 accagcgtag tggccccggt ggtggtcagg ccagagaaag attcaaaaaa cgcatcggta
     1741 atcgtgaggt tcgggctttc cgagaagata aaagggagcg caccgacact acccagcacg
     1801 gtccagaaca gcaccactat cagaaacccc tcacgggatt tcagttcgcc cttctcttta
     1861 cggttcggcc accacagcat agaaccaatg gcgagggcga caaaaaaggt ctgggtaaaa
     1921 gcgcggcccg ctccatcccg gtagatgagt gctaccagcc cagggataat catggtccct
     1981 gaaaataaga tgaccagtag tccaacgatt cgggtaatgg cgcgaaaatg catctctgcc
     2041 gcttccttag ttctgcaaaa cgaagtgggg attattcttc aatcgctaac aattgcaatg
     2101 aaccacggct aaaatccgcc agctttgcgg aaaattcagc cactttcgcc gccggaagcg
     2161 ccacccgcag cagaacgaat gcctgataat cactgttgat aattttgccg tcacactgcc
     2221 ccagcaacgc ttcaatgccg gttaactgat gatattcaca ttgcaaagta tattcggtta
     2281 atggcgtctt gcgttgggtc gttagctggc gcagcgcctg attcacgccg ccgccatacg
     2341 ctttcactaa cccaccggtg ccaagcaata tgccgccgta gtagcgcact accacagagg
     2401 taatttcccc gacgccgctg cccattagct gggcgagcat cggtttacct gccgttcctg
     2461 ccggctcccc gtcgtcagag aaacccagct gttgagaatc atccggcgca cccgccaccc
     2521 acgccacgca atggtggcgg gcatcggggt gttctgcccg caccgattca acaaacgctt
     2581 tcgccgcctc aacgccatcg gtatgcgcca acatcgttat gaagcggctt tttttgatct
     2641 cttcaacgac cgtgaccggt gccgcaggaa ttaaccagct ttccatcacg ccagtttcag
     2701 atcccgggtc atgttttcca cgttgttttc gtggatcacc acgttgtctt cgatacgaat
     2761 gccgccgaac ggtttcagtg cttcaatttt ctgccagttg aagtgcttgc tgaactgccc
     2821 ttcacgccac ggtgccagta gcgattcaat gaagtagata cccggttcga tggttaacac
     2881 catacccggc tggagaatac gggtgcagcg caggtacgga tattttgccg gtgccgcgag
     2941 gtgtgtaccg ctatcatcct gcataaaacc ggctacgtca tgcacctgca ggcccagcgg
     3001 atgaccgata ccatgcggca taaacggtcc ggtgagatcg ttttcgacca tcgcttcttc
     3061 actcatatcg gtgatgattt gatgtttacg cagcagtttg gcgatgcgct gatggaactg
     3121 gatgtggtaa tccacatagc taacgcctgc tttcatggtg gcgatcagcg ccagttgttc
     3181 atcatttacg tctttcacca gctgtgcgta gtcgttgtcg ctttttgccg accaggtacg
     3241 agtcaggtca gccgcatagc cgttatattc ggccccggca tccagcagga agctgcgcat
     3301 ctcttccggt gcctgatgat ccagtttggt gtaatgcagc accgaagcgt gttcgttaag
     3361 cgccacaatg ttgctgtaag gtacgtcggt atcacgatga ccggtcgcgg tcagataggc
     3421 gatgttgata tcgaactcgc tcatgccaga acggaacgct tcttctgccg cacgatgacc
     3481 gttgaccgcc attttctgcg cttcacgcat acaggccagt tcgtactcgg ttttgaagga
     3541 gcggtagtaa tgcaggtagt cgatcacccc tttcgggttg atgttgctgg cctcaatacc
     3601 cagttgcagc gcacgttccg gcaccggacc gatataaccg atattgccgc gcgcagcggg
     3661 cagcaggcta ccaatgccat cggctttcgg cagtgcgatc acttccacat cttcagtcca
     3721 gaaggaggtc ggcagcggtt cgacgttgtg ccagtaatca accggcagat agaaccacag
     3781 tttcggcttg ttcacgccat ccaccagcaa ccagcagttt ggcacctgag ttaccggcac
     3841 ccacgctttg aattgcgggt tcactttaaa cggataggga tgatcgtcga gaaaaacgtt
     3901 gaacagctcg ccggagtgaa taagtaatgc atccagcttg aagcgcgcca gcgcatcgcg
     3961 agtccgttct tgtaaggtag ctatatgatt tttatagagc gaggccagtg attccatttt
     4021 ttacccttct gtttttttga ccttaagtct ccgcatctta gcacatcgtt catccagagc
     4081 gtgatttctg ccgagcgtga tcagatcggc atttctttaa tcttttattt gcatattttt
     4141 aacacaaaat acacacttcg atacatctgg tacgaccaga tcaccttgcg gattcaggag
     4201 actgacatgc tttacaaagg cgacaccctg taccttgact ggctggaaga tggcattgcc
     4261 gaactggtat ttgatgcccc aggttcagtt aataaactcg acactgcgac cgtcgccagc
     4321 ctcggcgagg ccatcggcgt gctggaacag caatcagatc taaaagggct gctgctgcgt
     4381 tcgaacaaag cagcctttat cgtcggtgct gatatcaccg aatttttgtc cctgttcctc
     4441 gttcctgaag aacagttaag tcagtggctg cactttgcca atagcgtgtt taatcgcctg
     4501 gaagatctgc cggtgccgac cattgctgcc gtcaacggct atgcgctggg tggtggctgc
     4561 gaatgcgtgc tggcgaccga ttatcgtctg gcgacgccgg atctgcgcat cggtctgccg
     4621 gaaaccaaac tgggcatcat gccgggcttt ggcggttctg tacgtatgcc acgtatgctg
     4681 ggcgctgaca gtgcgctgga aatcattgcc gccggtaaag atgtcggcgc ggatcaggcg
     4741 ctgaaaatcg gtctggtgga tggggtagtc aaagcagaaa aactggttga aggcgcaaag
     4801 gcggttttac gccaggccat taacggcgac ctcgactgga aagcgaaacg ccagccgaag
     4861 ctggaaccgc taaaactgag caagattgaa gccaccatga gcttcaccat cgctaaagga
     4921 atggtcgcgc aaacggcggg gaaacattat ccggccccca tcaccgcagt aaaaaccatt
     4981 gaagctgcgg cccgttttgg tcgtgaagaa gccttaaatc tggaaaacaa aagttttgtc
     5041 ccgctggcgc ataccaacga agctcgcgca ctggtcggca ttttccttaa cgatcaatat
     5101 gtaaaaggca aagcgaagaa actcaccaaa gacgttgaaa ccccgaaaca ggctgctgtg
     5161 ctgggcgcag gcatcatggg tggcggtatc gcttaccagt ccgcgtggaa aggcgtgccg
     5221 gttgtcatga aagatatcaa cgacaagtcg ttaaccctcg gcatgaccga agccgcgaaa
     5281 ctgctgaaca agcagcttga gcgcggcaag atcgatggtc tgaaactggc tggcgtgatc
     5341 tccacgatcc acccaacgct cgactacgcc ggatttgacc gcgtggatat tgtggtagaa
     5401 gcggttgttg aaaacccgaa agtgaaaaaa gccgtactgg cagaaaccga acaaaaagta
     5461 cgccaggata ccgtgctggc gtctaacact tcaaccattc ctatcagcga actggccaac
     5521 gcgctggaac gcccggaaaa cttctgcggg atgcacttct ttaacccggt ccaccgaatg
     5581 ccgttggtag aaattattcg cggcgagaaa agctccgacg aaaccatcgc gaaagttgtc
     5641 gcctgggcga gcaagatggg caagacgccg attgtggtta acgactgccc cggcttcttt
     5701 gttaaccgcg tgctgttccc gtatttcgcc ggtttcagcc agctgctgcg cgacggcgcg
     5761 gatttccgca agatcgacaa agtgatggaa aaacagtttg gctggccgat gggcccggca
     5821 tatctgctgg acgttgtggg cattgatacc gcgcatcacg ctcaggctgt catggcagca
     5881 ggcttcccgc agcggatgca aaaagattac cgcgatgcca tcgacgcgct gtttgatgcc
     5941 aaccgctttg gtcagaagaa cggcctcggt ttctggcgtt ataaagaaga cagcaaaggt
     6001 aagccgaaga aagaagaaga cgccgccgtt gaagacctgc tggcagaagt gagccagccg
     6061 aagcgcgatt tcagcgaaga agagattatc gcccgcatga tgatcccgat ggtcaacgaa
     6121 gtggtgcgct gtctggaaga gggcattatc gccactccgg cggaagcgga tatggcgctg
     6181 gtctacggcc tgggcttccc tccgttccac ggcggcgcgt tccgctggct ggacaccctc
     6241 ggtagcgcaa aatacctcga tatggcacag caatatcagc acctcggccc gctgtatgaa
     6301 gtgccggaag gtctgcgtaa taaagcgcgt cataacgaac cgtactatcc tccggttgag
     6361 ccagcccgtc cggttggcga cctgaaaacg gcttaaggag tcacaatgga acaggttgtc
     6421 attgtcgatg caattcgcac cccgatgggc cgttcgaagg gcggtgcttt tcgtaacgtg
     6481 cgtgcagaag atctctccgc tcatttaatg cgtagcctgc tggcgcgtaa cccggcgctg
     6541 gaagcggcgg ccctcgacga tatttactgg ggttgtgtgc agcagacgct ggagcagggt
     6601 tttaatatcg cccgtaacgc ggcgctgctg gcagaagtgc cgcactctgt cccggcggtt
     6661 accgtcaatc gcttgtgtgg ttcatccatg caggcactgc atgacgcagc acgaatgatc
     6721 atgactggcg atgcgcaggc aggtctggtt ggcggcgtgg agcatatggg ccatgtgccg
     6781 atgagtcacg gcgtcgattt tcaccccggc ctgagccgca atgtcgccaa agcggcgggc
     6841 atgatgggct taacggcaga aatgctggcg cgtatgcacg gtatcagccg tgaaatgcag
     6901 gatgcctttg ccgcgcggtc acacgcccgc gcctgggccg ccacgcagtc gggcgcattt
     6961 aaaaatgaaa tcatcccgac cggtggtcac gatgccgacg gcgtcctgaa gcagtttaat
     7021 tacgacgaag tgattcgccc ggaaaccacc gtggaagccc tcgccacgct gcgtccggcg
     7081 tttgatccag taagcggtac ggtaacggcg ggtacatctt ctgcactttc tgatggtgcc
     7141 gcggccatgc tggtgatgag tgaaagccgc gcccgtgaat taggtcttaa gccgcgcgcc
     7201 cgtgtgcgtt cgatggcggt cgttggttgt gatccgtcga ttatgggtta cggcccggtt
     7261 cctgcctcga agctggcgct gaaaaaagcg gggctttctg ccagcgatat cggcgtgttt
     7321 gagatgaacg aagcctttgc cgcgcagatc ctgccgtgca ttaaagatct cgggctaatg
     7381 gagcagattg acgagaagat caacctcaat ggtggtgcga tcgcgctggg tcatccgctg
     7441 ggttgttccg gtgcgcgtat cagcaccacg ttgctgaatc tgatggaaca caaagacgtt
     7501 cagtttggtc tggcgacgat gtgtatcggt ctgggtcagg gtattgcgac ggtgtttgag
     7561 cgggtttaac cacttgccgg atgcggcgtg aacgccttat ccggtctacc gatcctgcac
     7621 caatgtaggc ctgataagac gcgccagcgt cgcatcaggc atcgtgcacc gattgccgga
     7681 tgcgacatag atctaaatgc cactgaataa agttttagtt gccgttcttc ccgcctgtca
     7741 ggggcgggtt tttatatctc agataaatgc aaacgcatcg ccaaacagac gatcttcccg
     7801 cgcattacgc tcactgcaaa acaggtcacg agcaattttc gccatctcaa aacgtccggc
     7861 aatatagata tcatgctctg ccagcgtacc atgatcctgc aagaccgccg ttaacacggt
     7921 gccagtacgc ccacgccagc ccgcttccgg ttgttcaacc accggcacca cttgcagacc
     7981 aggatgcttc agcgaaagcg cctcaagctc gcagagatca tacagatgtt gctcttcacg
     8041 cccaccccag taaatggtga tatcgcgatt gggattacgc gccaacgccg tcaataaaat
     8101 cgagcgcgca taggagaacc cggtgccgcc cgcaatcaaa atcatcggac gctcttcatc
     8161 atcgcgcagc cacgcttctc cgtggggaat atcgaccacg atttgatgat ctttgaggat
     8221 gcggtccatg actgctttcg cgtaaaggtt gatttcagaa gcgccaatat gcagctcgat
     8281 aaaccctttt tcatccggcg tcgaagccat tgaaaacgga cgtttgtcgc gctcatccat
     8341 cactaccatc aaatactgac cagcacgaaa agaaaaggcc gcgtctggta cgattcggac
     8401 acggtataca gtatccgtga tagcttctac cgaggtcact ttacagctta aggttgtcat
     8461 gcgctttctc tgtcggatca aataaatagg gcaaaactaa cgcgcatcag gcgcttttac
     8521 cgttgttaaa aatagccagt tcatcccaga tggcgtcaat atgcgcgacg acatcaggat
     8581 cttttttgat gggacgtccc cattcacgct gggtttcccc cggccattta ttcgtggcat
     8641 ccagccccat ttttgaaccc agcccggaga caggcgaggc aaaatccaga taatcaatag
     8701 gcgtattttc taccagaaca gtatcccgcg ccgggtccat acgggtggta atcgcccaaa
     8761 tcacatcgtt ccagtcgcgt gcgttgacgt catcatcgca aacgatcaca aatttagtgt
     8821 acataaactg gcgtaagaac gaccagacgc ccatcatgac gcgcttcgcg tgtccggcgt
     8881 actgtttttt gattgtcact accgccagac gataagaaca gccttccggt ggcaggtaaa
     8941 aatcgacaat ttccgggaac tgtttttgca gaatcggcac aaacacttcg ttcagtgcga
     9001 cacccagcac cgcgggttca tccggcggac gaccagtata ggtggagtgg taaatcgcat
     9061 cttcacgctg ggtaatatgc gtcacggtaa ataccgggaa actatcgact tcgttatagt
     9121 aaccggtgtg gtcgccatac ggcccttccg gcgcagtttc gccttgttcg atataccctt
     9181 ccagcacaat ctccgcactg gcgggcactt caaggtcatt ggagatacac ttcaccactt
     9241 cggtcttggt accgcgcagc aatccggcaa acgcatactc tgaaagtgta tccggaacgg
     9301 gggtgactgc accgagaatc gtggcgggat cggcacccag cgccacagaa accgggaaac
     9361 gttcgcccgg atgcgccgca caccactcct gataatccag cgcgccgccg cgatgcgaca
     9421 gccagcgcat aatcagtttg tttttaccaa tcagctgctg gcgataaatg cccagattct
     9481 gccgctcttt atgcgggccg cgcgttacgg tcagccccca ggtaatcagc ggcgcggcat
     9541 cttccggcca gcaggtcata atgggaatgc gattgagatc gacgtcatcg ccagagacga
     9601 ttttttgttg gcagggcgca ccacgcagcc gctttgtcgg catgttcaat acttgcttaa
     9661 actgcggcag tttatcaaac aggtcgcgga aaccttttgg cggctcgggc tctttcagaa
     9721 acgccaataa tttaccaact tcacgcagcg ccgaaacatc ttcctgcccc atgcccatcg
     9781 ccacgcgctt tggcgtaccg aacaggttgc acagcaccgg cattgagtag cctttagggt
     9841 tttcgaacaa cagcgcaggc ccaccggcac gcaaagtgcg gtcagcaatt tcagtgattt
     9901 ccagatgcgg atccaccggg agcgtgatac gttttagctc accctgctgt tcaagcagcg
     9961 tcaagaagtc gcgtaaatcg ttatatttca tggcgtccat tgtagcctct taatctacgc
    10021 ccattatacg gcgttcatct ttgcgatgct gtaaatttgt taaattagcg tgaactctga
    10081 cggtataacg caaaccgggg aatataatta acttagcgta aagcttttgc tatccttgcg
    10141 ccccgattaa acggataaga gtcattatgc aatcttggta tttactgtac tgcaagcgcg
    10201 ggcaacttca acgtgcccag gaacaccttg agagacaggc tgtgaattgc ctggcaccga
    10261 tgatcaccct ggaaaaaatc gtgcgtggaa aacgtactgc agtcagtgag ccattgtttc
    10321 ccaactacct gtttgtcgaa tttgatccag aagtgattca taccacgact atcaacgcga
    10381 cccgcggtgt cagccacttc gtgcgctttg gcgcgtcgct agcgatagtc ccatcggcgg
    10441 tgattcatca gctatcggta tataaaccga aagacattgt cgatccggca accccttatc
    10501 cgggagataa ggtgattatt accgaaggcg cgttcgaagg ctttcaggcc attttcaccg
    10561 aacccgatgg tgaggctcgc tccatgctat tgcttaatct tattaataaa gagattaagc
    10621 acagtgtgaa gaacaccgag ttccgcaaac tctaaaacgc aatcccaaac cgtgttttga
    10681 cattagcatc cgtggtggca gccagccatg cggcatcttc tccacgccag tgcgcaatac
    10741 gttgcaaaat atggggcaga tgggctggct cgttgcgccg ggatgatggc tttggcgtga
    10801 gatcgcgagg gagcagatac ggcgcatcag tttcgatcag taatttttcc gccggaatca
    10861 acggcaaaag ttcccgcagc tccagtccgc gtcgttcatc gtaaacccaa ccggtaatgc
    10921 cgatataaat tccatgcgcc acgcacgcct gcatctcttc gcgtgtgccg gtaaagcaat
    10981 gaagaaccgc accaggcagt ttatccagcc acggctccag caatgtcata aaccgctcgt
    11041 gggcatcgcg acagtgcata aataccggca tgtttaaatc tgcggcaatg cgtagctggg
    11101 caacaaaagc gcgttcctgc tcttccggcg tcgaaaagtt gcggttaaag tcgagaccac
    11161 attcaccaat cgccaccact tctggctgcg cggccagctc aataatcgct tcttcagtcg
    11221 cagcttgcca ctggctgctg tcgtgaggat gtacgcccgc cgttgaccaa caggacgaat
    11281 actgacgcgc cagcttttgc gcctgctggc tttcacgcag gttagtgccg gtgatgagta
    11341 gcccattaac tcccgcgtca aaagcgcgcg ctacaacatc atcacggtct ttcgcaaatt
    11401 gcgaactggt caaattaacg ccgatatcaa acatcctgta ctccatatga caaccgccct
    11461 gacgggcggt tgaatttatt cttcagtttt ttcgctttct gcttcagcgt cgttttcctc
    11521 ttcccgattt cgccctttac caacgtaaaa gcgtgagaag aagacaccga tttcaaacag
    11581 acagtacatc gggatcgcca atagcgtttg cgagaagaca tccggcggcg tcagcaacat
    11641 cccgacaacg aatgcaccaa ccagcacata cgggcgtttt ttgcgtaagt cttctggcga
    11701 ggtaatcccc atccagcaca gcagcacaat tgccaccggc acttcaaagg agacaccaaa
    11761 cgccataaac agcgccataa cgaagcttaa atagctggcg atgtcggtgg atacctgcac
    11821 cccttccggc gcggtattgg caaggaagcc aaatgccagc ggaaagacca caaagtaggc
    11881 gaatgccatg ccgatataaa acagcagaga gctggaaacc agcagcggca ccaccaggcg
    11941 acgttcatgc ttatacagcg ctggggcgat aaatgcccac acctgataga gaatcaccgg
    12001 cgctgacaga atcagcgaca ccataaaggt cagcttgatc ggcgtaaaga acggcgaggc
    12061 cacgtcggtg gcgatcatcg ttgaaccttg cggcaactgc ttgatcaatg gcgcggatac
    12121 caggtgatag atgtcattgg cgaaatagac cagacacagg aatatcacga tcaccgcgat
    12181 aatgcagttc agcagacgct tacgcagctc aattagatgc gtgataagcg gttgagtatc
    12241 ttctacagac atgtttacgg tttatcactc gacgaagggg aaggtgcagc ggttttcggt
    12301 tcagcgtccg cagcaggttt taccaccggc tctggcgtgg tttctggctt ctgttccggc
    12361 gaactggcct gcgtttgtgc agcggcaggc gttacgccct catgcgcagc ttcattatct
    12421 ttcaccaccg ggttatggat ggtgtgcgct tcatcgctcg ccttttcagg atcgtttgca
    12481 acgtaggaac gcttcatcga ctccgcggcc tggcgtagtt catccatcga cgctttcagt
    12541 tcgggcgtca ggttagtgag gctcgccttt tcaacctttt tcagactgtc ctgaaactcc
    12601 tggagtttta actcctgggt cagttcgttc tgcaccgttg tcgccagtga acgcaacgcg
    12661 cgaatccagc ccgctaccgt ttttaccgcc acaggcagtc gttgcggccc cagaacgacg
    12721 aggccgatga tgaacaccaa tagcagttcg ctaaaaccga tatcaaacac ggattacacc
    12781 tgctctttat cgtggcgctt cgcgtcttct gttttagcct gttcctgatt cgtatccgcc
    12841 tgcttatcgg cgatagtttt cgcagtaaaa tcagcatcct gactggtttt atcctgcttt
    12901 ggttcatcat cgctcattgc ttttttaaag cctttgatcg acgcaccaag atcggaaccg
    12961 atggagccga gctttttggt gccaaaaagc agtacaacga tgacggcaat aatcaataac
    13021 tgccaaatac tgataccacc catacatgtt cctctgtggt agatgatgat taaacaaggc
    13081 cgcattatac gttacacgac ccgccttgag cgatgaaaaa atcagcgtgt tttgcgccaa
    13141 ccgacaaacc aggcgatcag accacctgcc attaaccagc cgggcatcag cccccattca
    13201 ggtcggctga ccaacaagaa tgtgccactt aatactaacg tagcgccaat tccgagaaaa
    13261 taacgcgatt gtccctgacg tacatgattt gactgaagct cgcgggcaat cttatcaaca
    13321 ctgtgctgta aatacttgcc ctggcgcaaa ctgtcgtaaa ccaattcagg cagttctggc
    13381 attttttcga cccagaacgg cgctttttct ttaaatgctc tcaccagggc aggaataccg
    13441 acctgatctt taatccacga ctccaggaaa ggcttcgctg ttttccataa atcgagctgc
    13501 ggataaagct ggcgtcctac cccttcgaca tagagcaggg ttttctggag taacaccagt
    13561 tgcggctgca cttccatatt gaagcgacgc gccgtattaa acagatttaa cagtacatgt
    13621 ccaaacgaaa tttcggccag cggtttctca aagataggtt cacagaccgt acgaatggca
    13681 aattcgaact cttcaacgtt ggtatctggt ggcacccagc cagaatcgac gtgtagctct
    13741 gccactttgc gatagtcgcg attaaagaag gcgataaagt tttctgccag atagcgttta
    13801 tcttctttgt ttagcgagcc aacaatcccg caatcaatgc cgatatattt cgggttttcc
    13861 gggtgttcat agcttacgaa aatgttgcca gggtgcatat cggcatggaa aaagctgtcg
    13921 cgaaagacct gagtgaagaa cacctgcacg ccacgttccg ccagcaattt catgttggtg
    13981 ccatttttct ccagcgtcgc aacatcagac accggaatgc cgtaaatgcg ctccatcacc
    14041 atcatccctt cactacaata gtcagggtaa acttccggga tgtagagcat cgggctgtct
    14101 tcaaaatttc gccgaagctg aatggcgttg gcagattccc gcagcaaatt cagttcatca
    14161 atcaaggtct tttcgtactc gcgcaccact tcggttgggc gcagacggcg accatccggc
    14221 agcaaacgcg gcacccagcg agccagacgg tagataagtt tcagatccgc tttaataacc
    14281 ggcaaaatat ccgggcggat gactttaatc accacctctt taccattcga tttcaatcgc
    14341 gcggtatgaa cctgggcgat agaagcagaa gccagtggct tgatttcaaa atcgtcaaac
    14401 cacgcttcta ccggcaagcc gcccattgca gcttcaatct gctgcttcgc cagcttgcca
    14461 tcaaacggag caactttgtc ctgcaataac gccagctgat cggcaatatg cggtggaaaa
    14521 agatcgcggc gggttgataa catttgcccg aacttgatcc aaaccggccc cagttcttgc
    14581 agggccagtc gtagtcgctc acctaaaagt ttgtctttat gccgatttgg catccagaat
    14641 aatgagtatc gccatagccg tagcggcagg gtgatacgca ttttggggat cagttcatca
    14701 agtccgtagc ttaaaaaagt gcgaatgatg aaatataggc gccgtacttc acctggcgtc
    14761 atttagcctc cagtttttcc agccgtttgg tcagggcatc aacagcacgc tcgacggcag
    14821 ccgtctcttc cgcaaaccag gccacttcaa gcggaccggg tgccatacgc cactcttcag
    14881 taatggcttc cgccacataa cgttgctggc gcttaatgcc gtgatgcagg aactttgcgc
    14941 ctccgcgcat ggctttgctg attccttcag cggcgatatc accggtataa ggggccagca
    15001 gttccgcagg gtcgaactct gccagatctg ccagcgcaac gaagttttgc accacctgaa
    15061 tatcgccctg cacttccagc tcaccactgc gaatcagtgc ggtaagctgc tggcgatcgc
    15121 gaagtttcgg caacacactg gcgtaggcga taacggtgca gtcagcatcg cctgcccatt
    15181 cgcccagtac atcaacctgg cgttcgctga acaccagaat caatgacgtc gaaaagcctt
    15241 ttacctccac gcgcaatact ttacccagca gacgcgagcg ggctgttttc agcgcgggtg
    15301 agcgatacag gaaggtgttg agcagacttt caattcctgc cgtcactaaa ggtttaaaag
    15361 gcatttccgg tctcctgtca gaacttataa ccacgatgca gcgccacaac ccctgccgtc
    15421 agattgtagt agtcgacact ttcgaatccg gcatcctgca tcatggcttt cagggtatcc
    15481 tgatcgggat gcatacggat ggattctgcc agataacggt agctgtcggc gtcgttcgcg
    15541 accagtgagc caatacgcgg cagcacatgg aaggagtatg catcataggc tttgctcagc
    15601 ggctcgataa ttggcttcga gaactcaagc accagcaggc ggccgccggg tttcagcacg
    15661 cgatacattg aacgcagtgc tttatctttg tcggtgacgt tacgcagacc aaacgaaatg
    15721 gtgatgcaat caaaggtgtt atccgggaac ggcagcgcct cagcgttcgc ctgaacatac
    15781 tcaacgttgc caatcacacc gatattacgc agcttctcgc gtcccatttt gagcatggat
    15841 tcattgatat cagcaaggac cactttgcca gtttctccga ccaggcggga gaatttcgct
    15901 gtcaggtcgc cggtgccacc agccagatcc agcacggtct gcccacggcg tacgccgctg
    15961 caatcaatcg tgaatcgctt ccacaaacga tgaataccaa atgacatcaa atcattcatg
    16021 acatcgtatt ttgatgccac ggaatggaaa acgtgggcga ccatatccgc tttttgttcc
    16081 ttcgcgacgg tctgaaaacc aaagtgcgtc gtttcttgtg acttatccac catctcaatg
    16141 cctgctcatc aaaaaattgt tccagaagtg taacagattg ggcgtcgatg ccctagattt
    16201 ctacccggct taactacccc taatgggcta gcgcgactgc tgattatatt catcatcgcg
    16261 ttgataagct tcatcattcg gctgctccgg aaccgaccga agtcgatact cttcattctg
    16321 gctcaccgcc tgttcagcca aatccggatt aatctcgcgt ttaatttcta ctcctaaacc
    16381 gcgaaacgct tctgcctgcg ccagcacatt tccgcgacct gaagagagtt ttttcattgc
    16441 ctggcggtag ttatcctgcg ctttgtcgag actttgacca atcgcggaca tgtcatcgat
    16501 gaacaaacgc atcttgtcgt acagcttgct ggcacgatcg gcgatttgct gggcgttgcg
    16561 gctttgatgc tcataacgcc acaggttggc aatagtacgc aacgccacca gcagcgtagt
    16621 cgggctaacc agcatgatat tgtttttcaa cgcttcggtg atcagctccg gctggcggtc
    16681 aagcgccagt aaaaaagcgg gttcaacggg aataaacatc agcacgtaat ccagagttcg
    16741 cagccccggc agctgttgat aatctttgcg tcccagcaaa cggatatggt tacgcaccga
    16801 cgcgatatgt tcctgtagcg cgctttcgcg ggtgtagtcg tcttcggcgt taaaatagcg
    16861 ttcataggcg accagcgtca ttttggcgtc gatcaccaca tcttttccct gcggcagccg
    16921 cacgatgaca tccggctgca tccgcgagcg ggcgtcattt tcgatgctga cctgggtttc
    16981 atattcatac ccttcacgca gaccggaagc ctccagcacc cgcgtcaata ctacctcgcc
    17041 ccagttgccc tgggttttat tgtcgccttt cagcgcgcgc gtcaggttga tcgcttcctg
    17101 ggccatttgc gcgttgagtt gctggagatt gcgaatttcg tgggtcaggg tatggcgttc
    17161 ttgtgcttct ttaccgaagc tgtcctgaac ctgacggcgg aaaccgtcca gttgttcacg
    17221 tagcggcgac aacaggctgt tcagactctg acggttttgc tcatcaaccc ggcgattgct
    17281 gtgctcaaaa atacggttgg cgaggttttc aaactgctca ctgaggcgct gctcgctgtt
    17341 aatcatctgg cgaattttat cgtcagcatg ttgctgtgcg gcttccatcc gcgtggttac
    17401 ttcacgcaga tcggcctcca gagaggtgtt aatactttgc aggctgcgca cttcgttatt
    17461 gagtaactcg cactctgcac gccagtgctc gctttgggta atttgttgtt ttgccgcgct
    17521 taactccgcg accatctctt cacgttcagc taattgctcg gctttttgct gcgcatgttg
    17581 ataactggca aacagccagc caattgccac acccaccaac gcaataactg cgtaaaccat
    17641 gattgagaaa tccacaacgc ctcctgacgc aacaacttac ccgcacaaaa tagaattgtg
    17701 ctgtacaaac gtccagttgg aaaggcattt cctgcgaggc gctacgcaaa aatacaaaag
    17761 gccgaacgcg tcggccttca gacaggagaa gagaattaca gcagacgacg cgccgcttcc
    17821 accacgattt tcaccgcttg gctttcagtt tgtttcatcg tctcagcatt cgggatctct
    17881 tgctgggtgc ggttaacgat aacacccgct accataccgg cacgcaggcc ctgacttgca
    17941 cacatggtca gcagggttgc agattccatt tcatagttca ttatgcccat cgcctgccac
    18001 tcttccatag aacctttaaa gtgacgaact acgcgaccag agtaagtatc gtaacgttcc
    18061 tgacctgggt agaaggtatc agaagaagct gtcacgccaa cgtgagttgt cgcgccaatg
    18121 gatttcgcag cttcaaccag cgcagtcgta cattcgaaat cagcgacagc cgggaattcc
    18181 agcggtgcga agtgcaggct cgcgccatcc agacggacag acgccgtggt aaccaggaca
    18241 tcacccacat taatatgcgg ctgaatagcg cccgttgtac cgatacgcag gaaggtgcga
    18301 atgcccagct gtgccagctc ttcaacagca atagaggtag acgggccgcc gataccggta
    18361 gagcagacga taacaggttt accatccagc tctgcacgcc aggtagtgaa ttcgcggtga
    18421 gatgccagct taaccggctt atccatcagc gcggcgatct tttccacacg atccgggtcg
    18481 ccagggacga tggcaagcgt agccccttgt aaatcgtttt tagtgaggcc gagatgaaaa
    18541 acatcagact tggacatata caactcctct gtgaatcggt ttagtcagaa gaagcaaaaa
    18601 gacactttac cgaagggttt aacatttttt cgtgatactc atcaccatga cgcaaatgcg
    18661 ttgcataaat ttacccacca aaagtgattc aaatcacata aatcactctt cattctgcag
    18721 gactgcataa aagatgagcc gattcaggca atgtgatttg ttgcatgctg actatagtta
    18781 acattgcgca acatttttat aagggtacgg agaataccat ggcaacaaca caacaatctg
    18841 gatttgcacc tgctgcatcg cctctcgctt cgaccatcgt tcagaccctg gacgacgcga
    18901 ttgtggcggg cttcacctct atcccttcac aaggggataa catgcctgct taccatgcca
    18961 gaccaaagca aagcgatggc ccactgccag tggtcattgt agtgcaggaa atttttggcg
    19021 tgcatgaaca tatccgcgac atttgtcgcc gtctggcgct ggaggggtat ctggctatcg
    19081 cacctgaact ttacttccgc gaaggcgatc cgaatgattt tgccgatatc cccacgttgc
    19141 ttagcggtct ggtagcaaaa gtgcctgact cgcaggtgct ggccgatctc gatcatgccg
    19201 ccagttgggc ttcccgcaac ggcggcgatg ttcatcgttt aatgatcacc ggattctgct
    19261 ggggtggacg tatcacctgg ctgtatgccg cgcataatcc acagctaaaa gctgcagtgg
    19321 cgtggtacgg caaactgacg ggcgacaagt cgctgaattc accgaaacaa cctgttgata
    19381 tcgcaaccga tcttaacgcg ccgattctcg gcttatatgg cggtcaggat aacagcattc
    19441 cgcaggagag cgttgaaacc atgcgccagg cgctgcgggc tgctaatgcg aaagcagaga
    19501 ttatcgtgta cccggatgcc gggcatgcat tcaacgctga ttatcgcccg agctatcatg
    19561 ccgaatctgc gaaagatggc tggcagcgta tgctggaatg gtttaagcag tatggagaga
    19621 agcggagtta gtttcctctt agtctatatt tgagatggtg agtaaacgag tgcgttttgc
    19681 tgccatctct gtcccccaaa aaataatcag tatgaattga ttggagatta cgactccgct
    19741 atttgacctt aagaaagtcg gcaagtcata gtcataaatg agtctcttca atggaagaca
    19801 ccttagagat ttaccatcaa ctcccattca caaacaaaca ttaattatta tcaacacatt
    19861 cacattaatt ataaatcatc tcagaccttc ctgataaaaa gatattaaag tgaaacattt
    19921 tgtttttcac attttagttc atcactttta ggcgtaaata aaggtaaaaa aaacaacaat
    19981 actatttctc tttcacaaaa gaattaacaa aaggtttttc aaggatgagt gatattattt
    20041 acctgaccat aaccggagaa caacaaggat gtatttcctc tcgttgtgga acgagcgcat
    20101 caataggtaa tcgctggcag attggccatg atgatgaaat cttcgcgttc tcgctctcta
    20161 atagtattac gaatacaggc aaagggtcat aattgcatgg ccttagtttc tgtaaattga
    20221 ttgacaaaag ctccccgtta cttattaacg ctattaacaa taacgagcag ttgttcatgg
    20281 agtttgactt ctatcggatc aataggtttg gtcgatggga gaaatattac aacattcaac
    20341 tccgcggcgc gctcttatca gcaatcaatc acctgtttac tgagaacaat ctggatacag
    20401 aagccatcac tgtcagttat taatatatac tctccaggca tctgattgct aatactgaat
    20461 ttagttattg ggcatttccc gataactaca accgtctgtt tataccacgg ccaaaaacta
    20521 aagctggtaa tggtttcaaa acacttaata gtaaagcggt tggcagactg cttgctgctg
    20581 tcggcatata caatgggaat atcgaaggat tcagggaaac agcagacaag ttggggggag
    20641 atgcacctgc tggttatgat caatttatgg ataacaaagg gttactcatc gctggtgctt
    20701 caatagttgc tggagcagtt ctggggagaa tgagactccc tgatttggaa tcacttgagc
    20761 atttcggagc cagaggagca gtatcaggaa gaccatttaa tcccgagctt gccggtggtc
    20821 caatagaaaa cctgacgaca gatggcgtaa caatcaacag ggaaggtatc gctattgttg
    20881 aaaaacatat agccagattt ggtcatgatc ctgtaaatga agtgatgatc aatagactga
    20941 aagacattga gaaaggaaaa atccccccag aacaagtaga tcttaacttc tatactcatg
    21001 agtgtcggga atatcagcgt tactgtaatc ttggttggga aacaggacaa cctgatggtg
    21061 acgcaggcta tgcactctgg aatcatactg gatttgcccc tatatttcca gacatctgtt
    21121 atcacttaac ccattacaag cccgctgcc
//
LOCUS       DAJVYM010000064        21003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVYM010000064 DAJVYM010000000
VERSION     DAJVYM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.064"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..170)
                     /locus_tag="OQS60_002171"
     CDS             complement(<1..170)
                     /locus_tag="OQS60_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387920.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="OQS60_002172"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="OQS60_002172"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS2387921.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIIITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="OQS60_002173"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="OQS60_002173"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS2387922.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="OQS60_002174"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="OQS60_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS2387923.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OQS60_002175"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OQS60_002175"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS2387924.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OQS60_002176"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OQS60_002176"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS2387925.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="OQS60_002177"
     CDS             4288..5151
                     /locus_tag="OQS60_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS2387926.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="OQS60_002178"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="OQS60_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS2387927.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="OQS60_002179"
     CDS             6489..7106
                     /locus_tag="OQS60_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS2387928.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OQS60_002180"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OQS60_002180"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS2387929.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="OQS60_002181"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="OQS60_002181"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS2387930.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="OQS60_002182"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="OQS60_002182"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS2387931.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="OQS60_002183"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="OQS60_002183"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS2387932.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="OQS60_002184"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="OQS60_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS2387933.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="OQS60_002185"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="OQS60_002185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS2387934.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OQS60_002186"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OQS60_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS2387935.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="OQS60_002187"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="OQS60_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS2387936.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="OQS60_002188"
     CDS             18072..19781
                     /locus_tag="OQS60_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS2387937.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OQS60_002189"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OQS60_002189"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS2387938.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5082 a         5713 c         5385 g         4823 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattattatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcttg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAJVYM010000065        20714 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVYM010000065 DAJVYM010000000
VERSION     DAJVYM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.065"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OQS60_002190"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OQS60_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS2387939.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OQS60_002191"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OQS60_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS2387940.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OQS60_002192"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OQS60_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS2387941.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OQS60_002193"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OQS60_002193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS2387942.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OQS60_002194"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OQS60_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS2387943.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OQS60_002195"
     CDS             complement(8217..9110)
                     /locus_tag="OQS60_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS2387944.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OQS60_002196"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OQS60_002196"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS2387945.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OQS60_002197"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OQS60_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS2387946.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OQS60_002198"
     CDS             complement(10241..11473)
                     /locus_tag="OQS60_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS2387947.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OQS60_002199"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OQS60_002199"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS2387948.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OQS60_002200"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OQS60_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS2387949.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16542)
                     /locus_tag="OQS60_002201"
     CDS             complement(14278..16542)
                     /locus_tag="OQS60_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCS2387950.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16749..17033)
                     /gene="ihfB"
                     /locus_tag="OQS60_002202"
     CDS             complement(16749..17033)
                     /gene="ihfB"
                     /locus_tag="OQS60_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS2387951.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17193..18866)
                     /gene="rpsA"
                     /locus_tag="OQS60_002203"
     CDS             complement(17193..18866)
                     /gene="rpsA"
                     /locus_tag="OQS60_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS2387952.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTVVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18977..19660)
                     /gene="cmk"
                     /locus_tag="OQS60_002204"
     CDS             complement(18977..19660)
                     /gene="cmk"
                     /locus_tag="OQS60_002204"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS2387953.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19833..20597)
                     /gene="ycaL"
                     /locus_tag="OQS60_002205"
     CDS             complement(19833..20597)
                     /gene="ycaL"
                     /locus_tag="OQS60_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS2387954.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5733 c         5045 g         4965 t
ORIGIN      
        1 cgtaatgtgc cactttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tctcctgaga aggcgcttcg tcacttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acgtgttcgg tattctggaa
      181 gactttacgc accagtttat aggtggtgcc tttctccggg ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccagc catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 cagcgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tcagacgtac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgttcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttcg cgggaggtta actcctcggt cagtcggcta agttcgattt ccatctgctc
      901 gatggcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgttccgga cgttttggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacccaatgc
     1081 cttatccgac atagagcgca aatcatcagc ggagagataa gccagctcgc gacggtgtaa
     1141 gcgccgttca acgacgttat ctttcaccat gcgcatcact gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 cagattgtcc atctccgctt cgcagaaagt cagtgctttt tccagctggt tgcggcggga
     1321 ccggttatta ctcagttgcg catgcagctc gtcacggcga attcgcgccc gctcttctgc
     1381 cccgctatcg gcgcgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccacgcaacg cttcacgagc gcgagtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agcttttcgt tgagatcgct attaccgctc agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgcct gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt agctgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga cgcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gacgatcggc gattttcacc actaccgctt tttccagctc
     2281 atcaacgctg aacacgctgt catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcagttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagcgc tgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacgcc gccaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cagcgttttt
     2581 gcgcgcgccc acttcatcgc gttcaacaat cgcctctcgc tcacgctcca gcaactgttg
     2641 cagatattcg gtgacgtcct ggctggaggt aaactcttca ccgcactgct cgctcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggcgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctggcgcag tgccatgcgc tcttcacggg cgttagacac
     2821 gctatcggaa agagaggcaa tgcgcgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaagtctgcc agcaggcgtt cggcttcttg
     2941 ctgctcgcgc agacgctgtt ccagttcact taagcgcatc cgcaacggtt gaacctgctc
     3001 tgccaggtga cgctgatcga ccccttcgcg cagcagttcg cgggcgacat cccaagcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 ctgctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cgcgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcgttctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcgttttgct cttccagacg gatctgcagc tcatcgagat ccgcttcgta
     3481 gcgttcgatc ttctcctgct gacgctgtgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtaactcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatggt ggtggattta cccgccccgt taccgccaga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgattctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcgaaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagttttgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccga catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccccagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacacgccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaatga cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccaccg cccgcatcca gcacacgcag tttttgcgga
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgcgaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gaaggtattc
     7801 gcttatggcg cgaaaatccg ggatcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtgccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gagttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggcg
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccaag ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccccttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagtga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt tgtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcaa cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca acgccgaggt
    12721 agcttcgtcc agaatcagaa tcgggctatc acgcaacaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ctatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcgaccg ttttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggcacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat tagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcgctggtg gtcacctgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgataca acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgattgacg caccttcacg
    13801 cacaaccgtt atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaaaaat gaaaccggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agcccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttaaaaggcg caatggttgg ccacagtcgg cggaacgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctccct
    14341 gaggctgcta atccgccagc cttgcgctga aaaattgacc gctatttgcc cctgatgagg
    14401 agtatcaagc cacttataac cctgttgttg atagcgatgt ttaactttgc ttgagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgtctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagcg gggacttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccttt
    14701 atcgttgcta gcttgtaaag gccagtgcac gctgaaacgc aatccttgcc attgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatgtg tgcaatattg agtccagccc tccccggtga tccagatgtt catgactcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcgggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac acctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctaca gccacacaca ttgctggcaa
    15121 cgttcgccag gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacgt tcagcaatgt tgatccaccc ttctggcaaa ctctttaacc cccagaaaag
    15241 taaagccaaa gaccggtcgg caagaaacca taacccttgc tccaggatta acggcccgct
    15301 taaatgcaca accatcgcgg cgaggatcaa cggaaccgtg ataaatgtca ccaagggaat
    15361 tgctaataga tttgcaataa acgaggtcag actaatgcca tgaaatatga cgatttgcac
    15421 gggcataagc agaagtgtga tacccagttg cagatggatg agggaaacaa ctgcacgcaa
    15481 taccggcggc agttgccact caggacaggg aaaccactga taccaaaaaa tcagtgccgc
    15541 gaccgcagcg gcagagagcc ataaactttg cgagagaatg gcaacaggat ccatcagcaa
    15601 aattgccgcc agacaacata tccatacatc ccagccactc cactgtcgcc cacttaactt
    15661 aagcattccc cacgtagcaa gcgccaccac ggtacgcaat gcaggaggtt gcattcccgt
    15721 caaccaggca taaaaagcag cacagcagat cccaccaatt aatggtattt gccagtggat
    15781 ccagcgccca ggcagaaaaa tttgtccact gcgaatgagt ccggcagcca gcaacaccgc
    15841 aaaagcgatg tgcaatcccg atatcgccat taaatgcgcc gttccagtat cgcgcatgat
    15901 atttttgatt tctttgggga cggataaccg ttcccccata cctaaaccaa gaataaccgc
    15961 attccacgga tagggttgca gcgttgtttg tagtgacgcc agatactgtg cacgcaggct
    16021 acaattcggt tcaataagac ttgcctgcag aaagcggccg gtgagcggct gatgctgggc
    16081 aatggcataa cgctggctat caaagccgcc atcattaagt tgaccatgaa cggcacgaac
    16141 tttgagtttc attgaccaca gttgtccggc acaaaccgct tgcggaagat attcgccata
    16201 cagcacaagg cctggcgcag ggaatatacg tcgaccttgt agataagtaa tttgaccgta
    16261 atgagtggtc atgccgtcag tagcagtgat ctcaacaatt gcatcctgcg tcgcgccagt
    16321 taaggtttct cctgcccaca aaatttgctt tgctgataat atgccccaaa cgaaaaacag
    16381 caacgtcagc gcgatataac ggacggtttt aacaggtata aatgccagta cgcaggcgaa
    16441 gagagtcaga aacgcaaggg ctaatgtccc aggcaattgg ggcaaaatca gcaacggaaa
    16501 aattccgcaa attatgcata cgtcgactgt cgttattttc atgcactcct ccctgtttat
    16561 ggaaagtgtg caactttgtt gagcggttgt ctggtgggaa ttttgcgttc cccgaggcac
    16621 attcagaaga atttaacgag agggtgaaaa ctgctcaaaa ctctgcgagg cagcttccag
    16681 ttcctgatta gagaaatgaa aaaagcaccc gacaggtgct tttctcgcgt tcaagtttga
    16741 gtaaaaactt aaccgtaaat attggcgcga tcgcgcagtt ctttaccagg tttaaagtga
    16801 ggaacgtatt ttccttccag ttctacttta tcgccagtct tcggattacg tccggtacgt
    16861 ggtgcgcggt agtgcaaaga gaaactgccg aaaccgcgga tttcaatacg ctcgccctgc
    16921 gcaagagtcg aggccatatg ctccagcatc tcttttactg catcttcaac cgtcttggcg
    16981 ggaatgtgcg attgctgggt ggcaagtctt tctatcaatt ctgacttggt catgattcct
    17041 ccggttcctt aaaggcaaat taagcggctg ctgttgctgc attgattaag ggcggccgta
    17101 gccgccctta gtgcttgatt acaggacgaa acctgcaatc tgtcaagtaa actcaacaaa
    17161 cttcggaata aaaatcccga agagtcagag aattactcgc ctttagctgc tttgaaagct
    17221 tcagccattg cgttgttgga gaagtttgca tcttcctgtt tgttaacagt tgcgattgca
    17281 tctttctcgt cagcttcgtc tttcgcacga acagacaggc tgattgcgcg gtttttacga
    17341 tcaacgccgg tgaatttagc ttcaacttcg tcgccaacgc tcagaaccaa ggtagcgtct
    17401 tcaacgcggt cacgagatgc ttcagaagca cgcaggtaac cttcaacgcc gtcagccagt
    17461 tctacggttg cgcctttagc gtcaactaca gttactttac cggttacgat agcgcctttc
    17521 ttgttcagag caacccagtt gttgaacgga tcttctgcga gctgtttaac gcccagggag
    17581 atacgttcac gttctgcgtc aacctgcaga acaactgcag cgatttcgtc gccttttttg
    17641 tattcacgaa ctgcttcttc gcctgcaacg ttccaggaga tgtcagacag gtgaaccagg
    17701 ccgtcgatgc cgccgtccag gccgatgaag ataccgaagt cagtgataga cttgatttta
    17761 ccttcaacac ggtcgccctt gttgtgggtt tccgcgaact gctgccacgg gttagctttg
    17821 cactgtttca gacccaggga gatacgacga cgttcttcgt cgatatccag aaccataact
    17881 tccactacat cgccaacgtt aacaactttg gacgggtgga tgtttttgtt ggtccaatcc
    17941 atttcggaaa cgtgtaccag accttcaacg ccttcttcga tttcaacgaa gcagccgtag
    18001 tcagtcaggt tggtcacgcg accagtcagt ttggtacctt ccggataacg tttagcgata
    18061 gctacccacg gatcttcgcc cagctgtttc aggcccaggg atacacgggt acgttcgcgg
    18121 tcgaacttca gcactttaac agtgatttcg tcgcccacgt tgacgatttc gctcggatgc
    18181 ttaacgcgtt tccaggccat gtcagtgatg tgcagcaggc cgtcaacgcc gcccagatca
    18241 acgaatgcac cgtagtcagt gaggttctta acgatacctt taacttccat gccttcctgc
    18301 aggttttcca gcagctgatc gcgctctgcg ctgttttcgg attcgataac ggcacgacga
    18361 gaaacaacaa cgttgttgcg cttctgatcc agcttgatta ctttaaattc aagctctttg
    18421 ccttccaggt gcagagtgtc acgcaccgga cgaacgtcta ccagagaacc tggcaggaac
    18481 gcacgaatac cgttcagctc aacagtgaag ccgcccttaa ctttgccgtt gataacaccg
    18541 gtaacagttt cagcatcttc gtaagctttt tccagcgtga tccaggcttc gtgacgttta
    18601 gctttctcac gggacagcag agtttcaccg aagccgtctt ctactgcgtc cagagcaacg
    18661 tcaacttcgt cacctacctg gatttccagc tcgccctggg cgtttttgaa ctgctcagcc
    18721 gggatggcgg actcagattt cagaccagcg tcaaccagta ctacgtcttt gtcgatagca
    18781 acaacaacgc cacgaacgat agaacccggg cgggtttcga tttcttttaa ggactcttca
    18841 aagagttgag caaaagattc agtcatgttt aatcttcagg tttatattta acgtccacct
    18901 ggctccatgc cggatggggt tgtttaacat acccgctgtc attccattgc aacgggggta
    18961 ctgcaaattc ggtcgcttat gcgagagcca atttctggcg cgcgtattgt agcgcttttt
    19021 caatcacttg ctcaatgctt aaggtggtgg aatccaacac taaagcatcg gatgccggaa
    19081 ccagtggcgc taccgctcgg ttacgatcgc ggtcgtcgcg ttctttgatc tcggccaaaa
    19141 ggcgctcaaa gttaacacta aagcccttct cctgcaactg tagcatgcgg cgatgcgcac
    19201 gttcttccga ggaggcgtca aggaaaattt tcactggcgc atcagggaat accaccgttc
    19261 ccatgtcgcg gccatcggca atcaggcctg gtaattcgcg aaacgcgcgt tggcgacgca
    19321 ataatgcttc acgaacgcgt gggaatgccg cgacttgtga agctgcattc gccacttcct
    19381 gagtacgaat ttcgccgctg acatcttccc cttcgaggat cacttccaga ttgccattgg
    19441 ttgacacaaa acgtacatcc agatgggatg ccagcggtac cagcgcatct tctgacgcaa
    19501 catcaacatg gtgatgtaat gccgccaacg ccagtacgcg ataaattgca cccgaatcca
    19561 gcagatgcca ttgcaatgct tccgccatag ccttacacaa ggtgcctttc cctgcaccgc
    19621 ttgggccatc aatggtaata accggggcaa ttgccgtcat ctttatctcc ttaaaacagg
    19681 cgtaccgtta acataaccgc gcgcattata cgcgccgacg ccacaaatcg ttaacgttgt
    19741 gtgcaaaaac cagttttgtc tgtattcaga tggggattta ttgcgcaatt atagcgggct
    19801 ggcagaagac cagcccgaaa gatgacaatg atttacttac cagaggcgat acgatcacgg
    19861 atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa acatggactg ggtgcgaccg
    19921 ccatccagac tagccagttt ttcaaagctg ccaaccagcc cctgggtgct gatgccacgt
    19981 ttcttcaaca gatcaaagga gaaatcatct gcttcggact ctttatcacg ggagtattta
    20041 gcattaatag cgccttctgc gatatcaccc aattgtgagc gggaaagctg ggaagccaca
    20101 ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg cataagaagc tttcatttca
    20161 gccagcgagt gacccaacgc gacgtggccc agttcatggc ccagaacacc ttcaatttcg
    20221 ttgtcattca tcatgtccat caggccactg tagacacgga cacagccgtt cgccatcgcc
    20281 catgcgttga cgtcgctggt catataaacc ttatagttga ccggcgtgcc attaatattg
    20341 ttacccaatg ctttggcgat ttttgccaga cgtttaccgt acttgctgga tttacttgcg
    20401 acttgattgc cgctgtccat ttcggcacag ccctgattgg caatcgcttt tgcatcggca
    20461 tcgcttaatg ttgctgcttt ataggcattt aaaccggagc tgatagccat attggtatca
    20521 ataccgtggg tattttgaca ccctgtcagt aatgctgcag aggtcgcaat cgccagcagt
    20581 aatttagtat tcttcatgtg aatcatccgt tgattaatca tatataaata ttatgtatca
    20641 acaacttaca ttgacaagcg acgctaccat taatcaaaat gactcacaag gtccgaaaaa
    20701 aaacgccgac aatg
//
LOCUS       DAJVYM010000066        20663 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVYM010000066 DAJVYM010000000
VERSION     DAJVYM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.066"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OQS60_002206"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OQS60_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS2387955.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OQS60_002207"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OQS60_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS2387956.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OQS60_002208"
     CDS             1894..2751
                     /locus_tag="OQS60_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2387957.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OQS60_002209"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OQS60_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OQS60_002210"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OQS60_002210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS2387958.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OQS60_002211"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OQS60_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OQS60_002212"
     CDS             complement(7411..8658)
                     /locus_tag="OQS60_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS2387959.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OQS60_002213"
     CDS             complement(8680..9006)
                     /locus_tag="OQS60_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS2387960.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OQS60_002214"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OQS60_002214"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS2387961.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OQS60_002215"
     CDS             10395..11651
                     /locus_tag="OQS60_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS2387962.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OQS60_002216"
     CDS             11766..12092
                     /locus_tag="OQS60_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS2387963.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OQS60_002217"
     CDS             complement(12233..13471)
                     /locus_tag="OQS60_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS2387964.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OQS60_002218"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OQS60_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS2387965.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OQS60_002219"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OQS60_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OQS60_002220"
     tRNA            complement(17456..17531)
                     /locus_tag="OQS60_002220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="OQS60_002221"
     tRNA            complement(17571..17646)
                     /locus_tag="OQS60_002221"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="OQS60_002222"
     CDS             17867..18226
                     /locus_tag="OQS60_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS2387966.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="OQS60_002223"
     CDS             18228..18620
                     /locus_tag="OQS60_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS2387967.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OQS60_002224"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OQS60_002224"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS2387968.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="OQS60_002225"
     tRNA            20346..20421
                     /locus_tag="OQS60_002225"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20467..20542
                     /locus_tag="OQS60_002226"
     tRNA            20467..20542
                     /locus_tag="OQS60_002226"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20500..20502,aa:Val,seq:tac)
     gene            20589..20661
                     /locus_tag="OQS60_002227"
     tRNA            20589..20661
                     /locus_tag="OQS60_002227"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20622..20624,aa:Val,seq:tac)
BASE COUNT         5132 a         5513 c         5141 g         4877 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aacatacttt atgtagtctc cgccgtgtag caagaaattg
    20461 agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    20521 tcgatcccgt catcacccac caatttctcg ccagctaaat ttcttgtaaa aatgtgaagt
    20581 accgaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    20641 gttcgatccc gtcatcaccc acc
//
LOCUS       DAJVYM010000067        20547 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVYM010000067 DAJVYM010000000
VERSION     DAJVYM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.067"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="OQS60_002228"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="OQS60_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS2387969.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="OQS60_002229"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="OQS60_002229"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCS2387970.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="OQS60_002230"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="OQS60_002230"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS2387971.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..4540)
                     /gene="araH"
                     /locus_tag="OQS60_002231"
     CDS             complement(3554..4540)
                     /gene="araH"
                     /locus_tag="OQS60_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS2387972.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4555..6069)
                     /gene="araG"
                     /locus_tag="OQS60_002232"
     CDS             complement(4555..6069)
                     /gene="araG"
                     /locus_tag="OQS60_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS2387973.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6139..7128)
                     /gene="araF"
                     /locus_tag="OQS60_002233"
     CDS             complement(6139..7128)
                     /gene="araF"
                     /locus_tag="OQS60_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCS2387974.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7925..8428
                     /locus_tag="OQS60_002234"
     CDS             7925..8428
                     /locus_tag="OQS60_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS2387975.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8507..8758)
                     /locus_tag="OQS60_002235"
     CDS             complement(8507..8758)
                     /locus_tag="OQS60_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS2387976.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="OQS60_002236"
     CDS             complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="OQS60_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS2387977.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9222..9545
                     /locus_tag="OQS60_002237"
     CDS             9222..9545
                     /locus_tag="OQS60_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS2387978.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9716..10213
                     /gene="ftnA"
                     /locus_tag="OQS60_002238"
     CDS             9716..10213
                     /gene="ftnA"
                     /locus_tag="OQS60_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS2387979.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10251..10490)
                     /locus_tag="OQS60_002239"
     CDS             complement(10251..10490)
                     /locus_tag="OQS60_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS2387980.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10681..11892
                     /gene="tyrP"
                     /locus_tag="OQS60_002240"
     CDS             10681..11892
                     /gene="tyrP"
                     /locus_tag="OQS60_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS2387981.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="OQS60_002241"
     CDS             complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="OQS60_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS2387982.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12815..12901)
                     /locus_tag="OQS60_002242"
     tRNA            complement(12815..12901)
                     /locus_tag="OQS60_002242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12865..12867),aa:Leu,seq:taa)
     gene            complement(12914..12987)
                     /locus_tag="OQS60_002243"
     tRNA            complement(12914..12987)
                     /locus_tag="OQS60_002243"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12953..12955),aa:Cys,seq:gca)
     gene            complement(13042..13117)
                     /locus_tag="OQS60_002244"
     tRNA            complement(13042..13117)
                     /locus_tag="OQS60_002244"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13082..13084),aa:Gly,seq:gcc)
     gene            complement(13269..13817)
                     /gene="pgsA"
                     /locus_tag="OQS60_002245"
     CDS             complement(13269..13817)
                     /gene="pgsA"
                     /locus_tag="OQS60_002245"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS2387983.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13874..15706)
                     /gene="uvrC"
                     /locus_tag="OQS60_002246"
     CDS             complement(13874..15706)
                     /gene="uvrC"
                     /locus_tag="OQS60_002246"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS2387984.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15703..16359)
                     /gene="uvrY"
                     /locus_tag="OQS60_002247"
     CDS             complement(15703..16359)
                     /gene="uvrY"
                     /locus_tag="OQS60_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS2387985.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16655..16831
                     /locus_tag="OQS60_002248"
     CDS             16655..16831
                     /locus_tag="OQS60_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2387986.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16818..17042
                     /locus_tag="OQS60_002249"
     CDS             16818..17042
                     /locus_tag="OQS60_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS2387987.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17109..17831)
                     /gene="sdiA"
                     /locus_tag="OQS60_002250"
     CDS             complement(17109..17831)
                     /gene="sdiA"
                     /locus_tag="OQS60_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCS2387988.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(18061..18813)
                     /gene="yecC"
                     /locus_tag="OQS60_002251"
     CDS             complement(18061..18813)
                     /gene="yecC"
                     /locus_tag="OQS60_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS2387989.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18810..19478)
                     /gene="tcyL"
                     /locus_tag="OQS60_002252"
     CDS             complement(18810..19478)
                     /gene="tcyL"
                     /locus_tag="OQS60_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS2387990.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19493..20479)
                     /gene="dcyD"
                     /locus_tag="OQS60_002253"
     CDS             complement(19493..20479)
                     /gene="dcyD"
                     /locus_tag="OQS60_002253"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS2387991.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         5254 a         5227 c         4729 g         5337 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga ccaaaggtgg
     3961 ttttattcag caacaaacca aagataatca gacacgcgac ggtgagccag attggcgcag
     4021 gcagaccgaa ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg
     4081 ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgttg
     4141 tgatcagagc atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa
     4201 tgccaagcag caaccccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca
     4261 ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg
     4321 aagcaaggca gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca
     4381 accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga
     4441 gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg
     4501 acttaggtgc gccagaaccc gatgtagaaa cagaagacat catactctcc ttactcaggc
     4561 aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc
     4621 gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc acaatccggt cggcaacgcc
     4681 gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc
     4741 ataaattacg ttatatattt cgtgcttagc accaacatca atgctgcgcg taggttcatc
     4801 cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt
     4861 tccgcctgag agattcatga tcagttgctc agcgcccggc gttttgatgt tgagcgaacg
     4921 aatgtgctga tcggcattgt tttcttccca accgttgttg attacacaac cgccgagcac
     4981 atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc
     5041 ttccgctttg cgatcttccg ggcagagcat catgcctgcg gcaatggcgt ggctcggttt
     5101 acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc
     5161 aaacaagcct ttcattaatt cgctacgccc cgcccctacc agaccaaaca gcccaacaat
     5221 ttcaccactg cgaaccgcca gacttattgg cgtacgcacg cctggtgctt tcacagcatc
     5281 aagacgcagg cgctcctcgc cataactacg aggttgccag ccgtagatat cgccaatgtc
     5341 gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt
     5401 tttgacataa cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc
     5461 catacggtga gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg
     5521 gaaaagattg tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat
     5581 tttagcgtta cgcgccagcg ccttggcgat ttcaaccatc tgccactgac caatggagag
     5641 atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc
     5701 ctcataattc agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata
     5761 gatgttttcc gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac
     5821 acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac
     5881 agaacccgtg gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc
     5941 gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt
     6001 cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga
     6061 ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag
     6121 gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa
     6181 gttgtcacgc gtgatcagca ccacgtcggt aacttcggta aattttggcg gttcaacgtc
     6241 ttttgctacc cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg
     6301 cagcagggaa ccgtagaagc cggttgcctg tgctttagac agttcgctca acgcatccac
     6361 accgttaatg ccaatgccga tgatatcggc agctttaaag ccctgacctt ccgtcgcgcg
     6421 tacgccgccc agcacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg
     6481 atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt
     6541 aggtacctga taaatttgtt tttccgggaa tccggccgct ttcagcgcat ccatagaacc
     6601 cgttgtacgg cgacgtgccg tatccagttc gttggcggta atcgccatca ccgcgctttc
     6661 tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctgaccct gacgttcgcc
     6721 aattttagtc gccgccatca tcaccagcgg aacggtatcc attggcttac ctttggcgtt
     6781 aacaaactgg tcatccacgg caatgacttt catatcgtag ccacgcgctt tcgcgacgat
     6841 ggcagagccg agtttggggt ccggagtaca aataacgaaa ccttttgcgc cactggcagc
     6901 caggctgtcg atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc
     6961 aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc
     7021 ttccggttgc ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat
     7081 aacggctgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag
     7141 ctttagtgtc gttttgtgta gggcaaaaac gaatgacttt cgttaaatta atcggaaaac
     7201 aaagcattac cttttatcta aaagataagt gaccgtgttg acatagtttt agcgagaaat
     7261 taattctcca taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt
     7321 tagcgggaaa agacataagg gaaagccaat ttgtcagaca aattgtcgaa tgcacagcag
     7381 attaatccat aagattagcc tggaaatcct tgttgtcttt ggtacccatg cgggatgtct
     7441 tctttttaac cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata
     7501 accgcaactc aaagatgtgg aaaatgcacg tcattcattt cgccattaat tatcactgtg
     7561 ctcattaatt aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt
     7621 ataaaatcat cacttcatca tgaattcaaa ttcattgatt aatatcaaca agatacaaaa
     7681 agcactatca ttaaaattca ttgcagttac attgatttca tcaatgaaat gtaaaaatat
     7741 ataaacttga tgatttaagc attttcttat acccgttcag acgttattct tatttcagat
     7801 catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa
     7861 acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt
     7921 aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta
     7981 cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac
     8041 cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa
     8101 ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa
     8161 actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag
     8221 tacgttggca cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt
     8281 cctgcgcgat ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga
     8341 tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct
     8401 gaatgtcgtg tcacaccagc tgcattgatc atcatcggcg ctaatgcatt gcgccgatga
     8461 aggttttgag aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac
     8521 cctgtggggt aaacttgagt tcaataagcg cgatggcttt ttggattgcc cgcatggtga
     8581 ccgggtctgc ggcggcggga tggttagtaa agtcgatatt cttcagctga ctggacattt
     8641 tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca
     8701 actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg
     8761 taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag
     8821 tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga
     8881 acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca
     8941 ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt
     9001 tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa
     9061 ataccccttt aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct
     9121 acagatggca agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta
     9181 acgcgtaaaa tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga
     9241 ctctttcatt aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag
     9301 ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc
     9361 ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata
     9421 cccacgcggt cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat
     9481 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
     9541 tctaaatatt ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt
     9601 ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa
     9661 ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct
     9721 gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact
     9781 gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt
     9841 cctgcgccgt cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac
     9901 tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc
     9961 acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa
    10021 cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg
    10081 gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag
    10141 cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga
    10201 cacacaaaac taatgctctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg
    10261 caaatcttac tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct
    10321 gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt
    10381 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
    10441 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
    10501 caaaaaaagc ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga
    10561 atgcttttta ttgtacattt atatttacac tatatgtaac gtcggtttga cgaagcagcc
    10621 gttatgcctt aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc
    10681 gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc
    10741 atgctggcaa tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg
    10801 attgggcttt gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat
    10861 gttccggcag ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa
    10921 tggctgacgg gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc
    10981 ggtgtcggtg aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca
    11041 accgctggcg tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg
    11101 ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg
    11161 ctggtattgc tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag
    11221 gggctggctc tgtcagcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg
    11281 ccgagtattg tcagctatat ggatggcaac gttcgtaagc tacgctgggt gtttataacc
    11341 ggtagtgcga tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt
    11401 gattcaacaa cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag
    11461 gcgttacgcg aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat
    11521 ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat
    11581 ttatttcagc gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg
    11641 ccgccgttgg cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc
    11701 ggtgtggcgc tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga
    11761 aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg
    11821 tttctctgtg gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca
    11881 gaagtggggt gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac
    11941 ccaatttggc ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc
    12001 aaggatcgtt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct
    12061 gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc
    12121 tctcttcaaa cgcttctggc gacatctttt ctacccgctc gaagttttcc tcagtaccgt
    12181 gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa
    12241 gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat
    12301 caacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg
    12361 cggtatcggc catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg
    12421 cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa
    12481 tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga
    12541 tggcgtggtt agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt
    12601 ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt
    12661 acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag
    12721 gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact
    12781 gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc
    12841 gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg
    12901 ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc
    12961 gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct
    13021 tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct
    13081 tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc
    13141 acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta
    13201 cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattacgc
    13261 cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac
    13321 cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg
    13381 ttcggacgcc acagcagcca tgccaacgcc accatctggg cagtggtttt cactttccca
    13441 atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca
    13501 gaaataataa tttcacgggc gatcatcgtt gctgccggta aggtcaccca ccagctgtgg
    13561 taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc tacagggtca
    13621 aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag
    13681 tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc
    13741 agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta
    13801 gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt
    13861 tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa
    13921 tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca
    13981 tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac
    14041 tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt
    14101 catcgcgaat atgctggata acatgcagcg cgggagaatc tggcggcaaa ctgaatccct
    14161 caccttccgg ctcaaagaac agcgtttcca gcccagcctt acgatctgct cctttggcaa
    14221 cgccaagtag cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt
    14281 tcgcctgcgc aagctggcct ttgccgccgt cgataaggat cacatccggg atcttactgt
    14341 cgtcaatggc tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc
    14401 ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat
    14461 caaacaccac acaggaagcg acggtttgtt cgcccatggt atggctgatg tcaaagcact
    14521 ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa
    14581 cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt
    14641 tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca
    14701 gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct
    14761 cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg aaggtttcta
    14821 ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca
    14881 ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac
    14941 caataacgtc gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg acgcgtcgca
    15001 ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa ctccagattc tggctggcag
    15061 tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc
    15121 gcacatactc aacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc
    15181 ccagacagcg ccctatctga tattgcagac acggacgcga gcgattgcga taaacactat
    15241 tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca cgtacggcat
    15301 agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca cgatgcatcg
    15361 ccagacgcgg gtgggtatcg ccactcagga agataaaagg ataagattta tcatcgcgta
    15421 gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt
    15481 cggtttctgt atgcgtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg
    15541 ttttgcgcga agcgaggttg ctgcggaaat agctggaaag ccgttttttc aggtctttcg
    15601 ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc
    15661 aggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt
    15721 ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat
    15781 gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag
    15841 ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc
    15901 acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc
    15961 catttgttga gcgatatcag aagcaatgta acgctgccct gaatagacag aacgaatcgc
    16021 actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat
    16081 gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt tgacatcagc
    16141 tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc
    16201 cattagcacc acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga
    16261 tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg
    16321 caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg
    16381 gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac
    16441 gttaaaaatg gtgggtattc gatttttctc tcgtttttgt aaattcgatt gtccacagtt
    16501 atggtcacgc ccgtcaccaa cggcttccgg gatcgtttca cagtaagccc ggcgatgaaa
    16561 tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat
    16621 taattaaccc gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta
    16681 tattaacgca tttaatagca ttgttgacgt cacgctcaaa aaacaaccac tgctattttt
    16741 agcaaagctt atggtatact ccgccgcctt aacatttttc accgcaaatt ttcattgcaa
    16801 catgacgagg aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac
    16861 tggcaaatga agtctcctgc ctgaaagcga tgctgacgct gatgctacag gcgatgggac
    16921 aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg
    16981 aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt
    17041 aatataaaac cggctgagag cgtgcctttc agccgatttt tgcatctggc acgcagaaag
    17101 aaaagcgatc aaattaagcc agtagcggcc gcgtaacagg ccacctgggt cttatttggt
    17161 gcattgattt ttttctgcat gtttttctga tggaaattga ccgtattctc agagattgac
    17221 aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt
    17281 tcgcgcttgc tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga
    17341 gccatcagac tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg
    17401 ggtatttcgc gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg atttggcagc
    17461 attaaatact gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc
    17521 tgtgcttcgc tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg
    17581 ttcagcacag gatcaattgc gagaaaattt tttgcctgat aataactaac ccacgactca
    17641 gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat
    17701 aacgaatagt aatcgtactc cagctgctga gcctgaagct ctatttcatg gtagacctct
    17761 tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc
    17821 gtatcctgca tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc
    17881 aaactatcac ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt
    17941 tgcaagccat cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt
    18001 atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta
    18061 ttattgcagt agaaacttct cgaggaactg gcgggtgcga ggctgctggg gatcagcaaa
    18121 taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaaa tcgcccggtc
    18181 cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg
    18241 cgccagctgg cggattgtgt tcaggacttc gcccaccagc tctggatcca gcgctgacgt
    18301 tggctcgtca aacaaaatca cctcaggacg cattgccagc gcacgcgcaa tcgctacacg
    18361 ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac
    18421 tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat
    18481 caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg
    18541 gaagacaaac ccgacgtgct gacgcaactg gcggatcagc gatttttgct gacttaatga
    18601 acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag
    18661 cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga taattgccac
    18721 cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtgaaattt
    18781 tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt
    18841 tgattctcaa aatggttctg caacgtcgat aatactgtcg ccatgatcca gtagatgagc
    18901 gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga
    18961 cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcctttac caggctgata
    19021 aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc
    19081 atagtctgcc atggcgtcat accaatactg gcagctgctt cccactgacc tttatcaata
    19141 gaagaaatgg cggcgcgcag tgtttcggcg gcataggcgg cagtgttcag cgacagacca
    19201 atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc
    19261 ataaacagtt gtgcgatgag cggtgtacca cgaaaaatag agatataaaa acgcgccagc
    19321 cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagcagtaag
    19381 ccaaaaaaca tgccgccgat actgagttgc agcgtatacc cagccccttt cagcaggaac
    19441 ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt
    19501 ggggatgata ggcgaacagc gcaggcgcac cgccggtatg aataaacaga atcggccctt
    19561 catctttgaa gcgtttctga ctgatacagc caatcagccc cgccatcgct tttccggtat
    19621 acacaggatc aagcagaatg ccttccagcc gcgccagcaa tttcactgct tccatgcctt
    19681 cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtata atttccgctg
    19741 atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaact
    19801 gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt
    19861 ccagcccaac agccagtccg gcgtgagttc cggcactgcc cgatgcgact accaccgacg
    19921 aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccta
    19981 gcgcgccaag agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt
    20041 cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgct tcgcacattt
    20101 caatctgggt attgaacaga tccagcaaca agcgattgcc gttggttaaa tagttttctg
    20161 cggttgtgcc aataggattt tccagcagcg ccacacagtg cagaccgagt ttcgccgcaa
    20221 ctgcggcagt ctgccgcaca tggttagact ggatcacccc ggcagtaatc agcgtatcgg
    20281 caccttcgcg cagagcatct gccgcgagaa attctagctt acgtaattta ttgccgccca
    20341 ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc ccgtcctaga taatcagaaa
    20401 agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg
    20461 ttaaattatg cagtggcata acagcctccg atgtatgttg ttgtgatttt cttattatgc
    20521 acgctgaaaa cgcgtaaata aaaaagg
//
LOCUS       DAJVYM010000068        20230 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVYM010000068 DAJVYM010000000
VERSION     DAJVYM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.068"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            689..1459
                     /locus_tag="OQS60_002254"
     CDS             689..1459
                     /locus_tag="OQS60_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS2387992.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="OQS60_002255"
     CDS             1450..1923
                     /locus_tag="OQS60_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS2387993.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="OQS60_002256"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="OQS60_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS2387994.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="OQS60_002257"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="OQS60_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS2387995.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="OQS60_002258"
     CDS             3359..3853
                     /locus_tag="OQS60_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS2387996.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="OQS60_002259"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="OQS60_002259"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS2387997.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="OQS60_002260"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="OQS60_002260"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS2387998.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="OQS60_002261"
     CDS             7698..8612
                     /locus_tag="OQS60_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS2387999.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10178
                     /locus_tag="OQS60_002262"
                     /pseudo
     CDS             8827..10178
                     /locus_tag="OQS60_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="OQS60_002263"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="OQS60_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="OQS60_002264"
     CDS             complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="OQS60_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS2388000.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12719..14008
                     /locus_tag="OQS60_002265"
     CDS             12719..14008
                     /locus_tag="OQS60_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS2388001.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14005..15471
                     /gene="hpaE"
                     /locus_tag="OQS60_002266"
     CDS             14005..15471
                     /gene="hpaE"
                     /locus_tag="OQS60_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS2388002.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15473..16324
                     /gene="hpaD"
                     /locus_tag="OQS60_002267"
     CDS             15473..16324
                     /gene="hpaD"
                     /locus_tag="OQS60_002267"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS2388003.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16334..16714
                     /locus_tag="OQS60_002268"
     CDS             16334..16714
                     /locus_tag="OQS60_002268"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS2388004.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16825..17628
                     /gene="hpaH"
                     /locus_tag="OQS60_002269"
     CDS             16825..17628
                     /gene="hpaH"
                     /locus_tag="OQS60_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS2388005.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17639..18427
                     /gene="hpaI"
                     /locus_tag="OQS60_002270"
     CDS             17639..18427
                     /gene="hpaI"
                     /locus_tag="OQS60_002270"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS2388006.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18602..19978
                     /gene="hpaX"
                     /locus_tag="OQS60_002271"
     CDS             18602..19978
                     /gene="hpaX"
                     /locus_tag="OQS60_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS2388007.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19988..>20230
                     /locus_tag="OQS60_002272"
     CDS             19988..>20230
                     /locus_tag="OQS60_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS2388008.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4763 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12481 atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt cctgatttta
    12541 tcaaaattga tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt
    12601 aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact acgcaatcgg
    12661 gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat
    12721 gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg catggcagga
    12781 agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc
    12841 gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaggtact
    12901 gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac gtgaagaaga
    12961 tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc cggaagagag
    13021 cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac
    13081 cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg ggcgtcctgc
    13141 cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga gcgccctgag
    13201 cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac aggcgcgcgt
    13261 ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc cgctggaaaa
    13321 tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc agccacaccc
    13381 gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt
    13441 taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca ctggcgataa
    13501 ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg agctggtggt
    13561 agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg
    13621 ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact accgccctaa
    13681 cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga
    13741 ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca
    13801 gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagtga
    13861 atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag gcttatctga
    13921 cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg
    13981 aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat
    14041 gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt gctggcggat
    14101 gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc
    14161 ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat
    14221 ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac cggcctgccg
    14281 atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga atttttcgcg
    14341 gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac
    14401 acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt accgtttatg
    14461 accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt actgaaaatg
    14521 tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga agccgatatt
    14581 ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga tgcgctggtt
    14641 cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg gcgcaacatc
    14701 atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc gccggtgctg
    14761 atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac catcttctcg
    14821 atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag catctacccg
    14881 gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat
    14941 ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt ctccggctat
    15001 atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga taaaccgtcc
    15061 gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct ggcagacgtt
    15121 gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg
    15181 tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg cctcgcgtcg
    15241 tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagctggc
    15301 atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg cggcgtaaaa
    15361 gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag
    15421 aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa
    15481 gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg aactgccagg
    15541 gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca gcaagcgttg
    15601 ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg tcaacagtgc
    15661 ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca
    15721 ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc agcttattgc
    15781 cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca gcctgaaact
    15841 ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact tcaaagtggt
    15901 ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc
    15961 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    16021 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    16081 gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc agttcaaaga
    16141 gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16201 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16261 caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgcttcccgc
    16321 gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc gcgaagaagc
    16381 cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg gtatttttcc
    16441 gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg ccgacgggca
    16501 gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca gcctggaaag
    16561 ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg ccgccctgat
    16621 ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga cgctgaattt
    16681 taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg tggcgctgcg
    16741 cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc gcagccgcat
    16801 ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct gatcgcccag
    16861 cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct ggattacccg
    16921 gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct gaaaatcgcc
    16981 gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat gcaggcaagc
    17041 tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt ccacgatggc
    17101 agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct ggctttcgtg
    17161 ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa cgccacggac
    17221 tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga tccggaaacc
    17281 cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc cggggtgatc
    17341 ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc cgccctgatg
    17401 tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa tcatccggca
    17461 aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact ggaagccggg
    17521 caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg cgacaccttc
    17581 cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg agagaacgat
    17641 ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat tatggctggg
    17701 gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact ggttgttgat
    17761 cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac aggcgattgc
    17821 gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc aaatcaaaca
    17881 actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg ctgacgaagc
    17941 ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg tgggcagtgc
    18001 gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag ccaacgatca
    18061 aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac cgcagattct
    18121 ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg ccgatatggg
    18181 ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg cgatcgtgca
    18241 gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac tggcaaaacg
    18301 ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc tgctcgcccg
    18361 cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga agcccggcgt
    18421 gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac cgtaggccgg
    18481 ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc ggataaggcg
    18541 ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta gaggaagaaa
    18601 aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc agcataaagc
    18661 gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga gcgtctttct
    18721 gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg ccggactcac
    18781 gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca ccctgttcta
    18841 tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg tcggtgcgcg
    18901 gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca ccatgtttgc
    18961 cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg aagccggctt
    19021 tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc gcgcccgtgc
    19081 caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga tcgtttccgg
    19141 ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc tgtttttgct
    19201 ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg atgactcacc
    19261 ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga tgatggataa
    19321 cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca tgcaacaacg
    19381 cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg cgtatttctg
    19441 cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc aaagttttaa
    19501 tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga tttgtaccat
    19561 tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa ggcatcacac
    19621 cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa ctgatcacaa
    19681 catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg caatggcgat
    19741 tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg gtattgcggt
    19801 gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg gctggttgaa
    19861 agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc tggtgattgg
    19921 tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga ccctgtaagg
    19981 aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt acgatgaaag
    20041 cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat tttttggccg
    20101 ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca atagcggaca
    20161 gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt ttgtcctgac
    20221 gccgccgtca
//
LOCUS       DAJVYM010000069        20148 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVYM010000069 DAJVYM010000000
VERSION     DAJVYM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.069"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1278)
                     /locus_tag="OQS60_002273"
     CDS             complement(<1..1278)
                     /locus_tag="OQS60_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388009.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
     gene            complement(1471..1540)
                     /locus_tag="OQS60_002274"
     tRNA            complement(1471..1540)
                     /locus_tag="OQS60_002274"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1506..1508),aa:Gly,seq:ccc)
     gene            complement(1619..2374)
                     /locus_tag="OQS60_002275"
     CDS             complement(1619..2374)
                     /locus_tag="OQS60_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS2388010.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2789..5086
                     /gene="xdhA"
                     /locus_tag="OQS60_002276"
     CDS             2789..5086
                     /gene="xdhA"
                     /locus_tag="OQS60_002276"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS2388011.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5097..5975
                     /gene="xdhB"
                     /locus_tag="OQS60_002277"
     CDS             5097..5975
                     /gene="xdhB"
                     /locus_tag="OQS60_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS2388012.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5972..6451
                     /gene="xdhC"
                     /locus_tag="OQS60_002278"
     CDS             5972..6451
                     /gene="xdhC"
                     /locus_tag="OQS60_002278"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS2388013.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6491..8269)
                     /locus_tag="OQS60_002279"
     CDS             complement(6491..8269)
                     /locus_tag="OQS60_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCS2388014.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8748..9935
                     /gene="ygeW"
                     /locus_tag="OQS60_002280"
     CDS             8748..9935
                     /gene="ygeW"
                     /locus_tag="OQS60_002280"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS2388015.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9993..11189
                     /gene="dpaL"
                     /locus_tag="OQS60_002281"
     CDS             9993..11189
                     /gene="dpaL"
                     /locus_tag="OQS60_002281"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS2388016.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            11247..12458
                     /locus_tag="OQS60_002282"
     CDS             11247..12458
                     /locus_tag="OQS60_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS2388017.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12511..13895
                     /gene="hyuA"
                     /locus_tag="OQS60_002283"
                     /pseudo
     CDS             12511..13895
                     /gene="hyuA"
                     /locus_tag="OQS60_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13943..14875
                     /gene="arcC"
                     /locus_tag="OQS60_002284"
     CDS             13943..14875
                     /gene="arcC"
                     /locus_tag="OQS60_002284"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS2388018.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14916..16541)
                     /locus_tag="OQS60_002285"
     CDS             complement(14916..16541)
                     /locus_tag="OQS60_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS2388019.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16589..17359)
                     /gene="yqeC"
                     /locus_tag="OQS60_002286"
     CDS             complement(16589..17359)
                     /gene="yqeC"
                     /locus_tag="OQS60_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS2388020.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17462..18040
                     /gene="mocA"
                     /locus_tag="OQS60_002287"
     CDS             17462..18040
                     /gene="mocA"
                     /locus_tag="OQS60_002287"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS2388021.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18362..>20148
                     /gene="ygfK"
                     /locus_tag="OQS60_002288"
     CDS             18362..>20148
                     /gene="ygfK"
                     /locus_tag="OQS60_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCS2388022.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5224 a         5180 c         4765 g         4979 t
ORIGIN      
        1 tcttttcgca tgtgaatttt gatcggtatc ggttttaata tcattgaacc aaaaatttgc
       61 cgactgataa ttatccttct cgtatctttt tataccatac ggacctgcta gttttttaac
      121 tatttttaga acctgctctt ttcttctggt gtttatcttt gcaagatttg caggataaat
      181 aacattaagt aaagccgcat cggcttctct gtaatgttta tctttttcca gataacctgg
      241 ggattctccc cctaaatcaa gctgtaacgt tattctttca taacctttat ctatgaggtg
      301 gttcaacctc gttgttgaaa gaggttcatc cagttcattg gctttagctt cgcgtagtaa
      361 atcagagaca aaaacagagt ctttcttcga gagtaagttc gacaaacgtt ccagtccaga
      421 ggtcaccagt gcaacggaag atgtgtttaa tcgggcatct tcttcccatg ctccggaatc
      481 ttccataaca tagaaattgg ctttatccag ataagcaatc agcagcgcaa ccgattttaa
      541 tcggtcacct ttttgccaat cttcagcgtt aattgtacca gtgtcgattg cttgtataag
      601 gagatcaaga taaagaccta acgcatcatt ttgtttatgg ttccacagct gcggcttgcc
      661 ttcttcctgc acatctgcca tcacggggga gttgctgtca aatcggatat gcacagcatt
      721 catttgcccg ggaactccat ccagtcgttt ggggttgcta atgacgtcct gcatccgctt
      781 aatctggtcg agcgtagaca tataatccca cagggtgagc agaacctttt ttgcggctac
      841 actatttccc tgatctgata ctaatgccat atagccccat aaactatccc tcagccagac
      901 agcatcatag tgggtttctg ccgctgtttt gtcatcttcg aaagtagaag caagaataaa
      961 tgttccccag ggtttctccg tcaacgttaa ctttcgtgtg ttttctgtta atgttaacaa
     1021 cccttgaatt tcgtctttgg tatagtggct ctttatttca ttttttaatt tggctgagtg
     1081 aatcagaaga cgataattag ggtacttatc tggatttaac gataaataac ttttatctgt
     1141 tgctgccccc gtggaattaa gaatgttaaa tacagattca ctcgtcactt ctgcccctgt
     1201 cgcctggcat gtgccgcacg aaaatatacc agcgagcaga agagttaatg cagatttccc
     1261 tttcataaaa tattccatca atcgtttgtt aaaagcgtaa ataaccactt atttaacaac
     1321 ttgaaaataa ggtgttttac ctgggttgtt acaaaaggat tgcattgcgt aaacgctttt
     1381 tatttacaac aaaatgggga aatattacgg cgattgcgaa aagtctgaag cgattgaatt
     1441 aagataatat agaaaatcag atacagcaaa agcggcgaag ggaatcgaac cctcgtatag
     1501 agcttgggaa gctctcgttc taccattgaa ctacgcccgc ttcgagatgc gtaaggcatt
     1561 ataaacctta cgctctcctt agcaagtgcc gcgctgctga ctgctgatta attcgccatc
     1621 agcattttgg cttgctgccc tgcggcggca agtaacgtag cggatcaatt gccgttgcac
     1681 ggtaacgaat ctggaaatgc aggcgaacag atgccgcatc cgtgctcccc atagtggcga
     1741 ttttttgccc agccttcacg ctttgcccat tatttaccag catcgtgtca ttatgggcgt
     1801 aagccgtaat gtaatcttca ctgtgtttaa tcatgatgag attaccgtag ccacgcagct
     1861 ggttgcccac atacaccacc tttcctgcac ccgcggcgta aataggtgta ccccgtggag
     1921 ctgagatatc aatcccttta ttgccgccat ctgctgtcga atacggcatg ataactttcc
     1981 ctgtcgttgg ccataaccaa caacgttgcc ctactggcgg ccaggatgat ttcggtaccg
     2041 ctgatgacgg tgtaaccgat gcggttttgg tcgttgattt ggcggttgat ttacgtgtac
     2101 tactgctact tttcgcccca cccagtttta gtttctgacc aacttcaatg gtgtaagggg
     2161 gggaaatgcc gttcagtcgc gccagttctt ttacgctggt tcccgtggtg cgcgaaatac
     2221 gatatagcgt atccccccgt ttcacggtgt aaacggagcc ggaatacgtt cctgtatcgg
     2281 atgatttgct acccgaacag cccgccaaaa gcagtccaac cgataacaac atcacgatac
     2341 ccagagattt tttattcagg cgtcccgcac tcaaaatctt tcctcgctaa aaaaacaaga
     2401 cgccttatca tagcagccag cctcttgata cccaatctct tgcttctgga aaaacgtggt
     2461 aatagagaaa gaggttaatt tcatttggca aagggaagta tcatcagcaa aatattcgct
     2521 gtagaggtat cgaacttcct gactttttac cctatttccc atcctgatgc gtaataatcc
     2581 caatttatca cattgatttt atcgccactc atattgatac gtatcactgc ccctattcag
     2641 agtcctgttt cgtatcatat ctgtgttttt ttaacgtcat cacacttcct acctccccta
     2701 acgcttatcg tcgtttctgg cgtaaatctt gcctgcttag actaaatctt tgccataaga
     2761 accaaatgtt caaatcgagg ggatttctat ggaagcgcgg gaagcaaccg ctacgggtga
     2821 atcatgcatg cgcgtcgatg ccattgctaa ggtcaccggg cgggcacgat atactgacga
     2881 ttatgttatg gcgggcatgt gttatgcgaa atatgtacgt agccctatcg cacatggtta
     2941 tgccgtaagt attaatgatg aacaagccag aagtttgccg ggcgtactgg cgatttttac
     3001 ctgggaagat gtgcctgata ttccattcgc tacagctggg catgcctgga cacttgacga
     3061 aaacaagcgc gataccgccg atcgcgcact gctaactcgc catgttcgtc atcatggtga
     3121 cgccgttgcc atcgtcgtgg cccgcgatga actcacggca gaaaaagcgg cgcaattggt
     3181 cagcattgag tggcaagaat tacccgttat caccacgcca gaagcggcgc tggcagaaga
     3241 cgctgcacca atccataacg gtggcaattt actgaaacaa agcacgatgt cgacgggtaa
     3301 tgtccaacaa acaatcgatg ccgccgacta ccaggtacag gggcactatc agacccccgt
     3361 tattcaacat tgtcacatgg aaagcgtaac atcgctggcg tggatggagg atgactcgcg
     3421 aattaccatc gtttccagca cccagatccc gcacattgtt cgccgcgtgg ttggtcaggc
     3481 gctggatatt ccctggtcat gcgtacgagt catcaaaccg tttgtcggtg gcggttttgg
     3541 taataaacag gatgtactgg aagagccaat ggcagcattc ctgaccagca agcttggcgg
     3601 cattccggtg aaagtttccc ttagccgtga agagtgtttc ctcgcaaccc gtacccgcca
     3661 cgcttttacc attgacgggc aaatgggcgt gaaccgcgac ggaacattga aaggttatag
     3721 tctggatgtt ctgtctaaca ccggcgctta tgcatctcac gggcactcca tcgcttctgc
     3781 ggggaggaat aaagtcgctt acctttatcc tcgttgtgcc tacgcttaca gttcaaagac
     3841 ctgctatacc aacctcccct cggctggtgc gatgcgtggt tatggcgcgc cacaagtcgt
     3901 atttgccgtt gagtctatgc ttgatgacgc cgcgacagcg ttaggtattg atcctgttga
     3961 aattcgttta cgcaacgccg cccgcgaagg agatgctaat ccgctcacgg gcaaacgtat
     4021 ttacagcgca gggttgccgg agtgtcttga aaaaggccgg aaaatctttg aatgggaaaa
     4081 acgccgtgca gaatgccaga accagcaagg caatttgcgc cgcggcgttg gcgtcgcctg
     4141 ttttagctat acctctaaca cctggcctgt cggcgtagaa atagcaggcg cgcgccttct
     4201 gatgaatcag gatggaacca tcaacgtgca aagcggcgcg acggaaatcg gtcagggtgc
     4261 cgacaccgtc ttctcgcaaa tggtggcaga aaccgtgggg gttccggtca gcgacgttcg
     4321 cgttatttca actcaagata ccgacgttac gccgttcgct cccggcgcat ttgcctcacg
     4381 ccagagctat gttgccgcgc ctgcgctgcg cagtgcggca ctattattaa aagagaaaat
     4441 catcgctcac gccgcagtca tgctacatca gtcagcgatg aatctgaccc tgataaaagg
     4501 ccatatcgtg ctggttgaac gaccggaaga gccgttaatg tcgttaaaag atttggcgat
     4561 ggacgctttc taccaccctg aacgcggcgg gcagctctct gctgaaagct ccatcaaaac
     4621 caccactaac ccatcggcgt ttggctgtac ctttgttgat ctgacggtcg atattgcgct
     4681 gtgcaaagtc accatcaacc gcatcctcaa cgttcatgat tcagggcata ttcttaatcc
     4741 actgctggca gaaggtcagg tacacggcgg aatgggaatg ggcattggct gggcgctatt
     4801 tgaagagatg atcatcgatg ctaaaagcgg cgtggtccgt aaccccaatc tgctggatta
     4861 caaaatgccg accatgccgg atctgccaca actggaaagc gcgttcgtcg aaatcaatga
     4921 gccgcaatcc gcatacggac ataagtcact gggtgagcca ccaataattc ctgttgccgc
     4981 tgctattcgt aacgcggtga agatggctac cggtgttgca atcaatacac tgccgctgac
     5041 gccaaaacgg ttatatgaag agttccatct ggcaggattg atttgaggat aacatcatgt
     5101 ttgattttgc ttcttaccat cgcgcagcaa cccttgccga tgccatcaac ctgctggctg
     5161 acaacccgca ggccaaactg ctcgccgatg gcactgacgt actgattcag ctccaccatc
     5221 acaatgaccg ttatcgccat attgttgata ttcataatct ggcggagctg cggggaatta
     5281 cgctggcgga agatggctcg ctacgtatcg gctctgcaac gacatttacc cagctaatag
     5341 aagctcctat aactcaacgt catctcccgg cgttatgtgc tgcggccacg tccattgctg
     5401 gaccgcagat ccgtaacgtc gctacctacg gtggaaatat ttgcaacggt gccaccagcg
     5461 cagattctgc cacgccaacg ctaatttatg acgcgaaact ggagatccac tccccgcgcg
     5521 gtgttcgttt cgtcccgatt aatggctttc acaccgggcc gggcaaagtg tctcttgagc
     5581 atgacgaaat cctcgtcgcc tttcattttc cgccacagcc gaaagaacac gcgggcagcg
     5641 cgcattttaa atatgccatg cgcgacgcaa tggatatttc aacgattggc agcgccgcac
     5701 attgccgact ggataacggc aatttcagcg aattacgcct ggcatttggt gttgccgcgc
     5761 caacgccgat tcgctgccaa catgccgaac agactgcaca aaatgcgcca ttaaacctgc
     5821 aaacgctgga agctatcagc gaatctgtcc tgcaagatgt cgccccgcgt tcttcatggc
     5881 gggccagtaa agagtttcgt ctgcatctca tccagacgat gaccaaaaaa gtgattagcg
     5941 aagccgtcgc cgcggcgggg ggaaaattgc aatgaatcac agcgaaacaa ttaccatcga
     6001 atgcaccatt aacgggatgc cttttcagct tcacgccgca ccaggcacgc cgctctcgga
     6061 attactccgc gaacaaggac tgctaagtgt caaacaaggg tgctgcgtgg gtgaatgtgg
     6121 tgcctgtacg gtgttggtcg acggcacagc aatagacagt tgcttatacc ttgccgcctg
     6181 ggctgaagga aaggagatcc gcacgctgga aggtgaagcg aaaggcggaa aactttctca
     6241 tgttcagcag gcttatgcga aatccggcgc agtgcagtgc gggttttgta cgcctggcct
     6301 gattatggct accacggcaa tgctggcgaa accacgcgag aagccattaa ccattacgga
     6361 aattcgtcgc ggactggcgg gaaatctttg tcgctgcacg gggtatcaga tgattgtaaa
     6421 tacagttctg gattgcgaga aaacgaagta aaaggatatc cggcctgaat tcaggccgga
     6481 ttcactgagg ttatgtgttt aacaactcat atttcttaat cttgcgatag agcgtagcaa
     6541 tgccgatgcc cagttcatca gcaacttgct tcttgctgtt atgacgtgaa agcgcctcgc
     6601 ggatcatttg cttttccatc tcctccagcg ccgtgccgcc cgcatcatcg agtgacaggt
     6661 gcgcctcact gacctctgtt acatcacttt gctccgttgt gccattattc agcagatttg
     6721 gcggcaatag cgtgctgtcg ataacttcac ctgaaggaac cacgttaacc agatattcca
     6781 tcaaattgct taactcgcgc aggtttccgg gccaacgatg cttacgcaat atttcgacga
     6841 catcgggagc aatgccagga taaaccgatc ccagacgacg ggtatgcaga tgtaaaaagt
     6901 aatgcaccaa tagttcaata tcttcctgac gttcacgcag cggtggcaga gttatcggga
     6961 taacattaag tcggtagaag agatcttcgc ggaatttacc ttcggcaatg aactgggcca
     7021 aattctgatt agttgcagaa atgatgcgaa tgtcgacttg tattgggcta ctggcaccaa
     7081 tcggcagaat ttcacgcgcc tcaatagcgc gcagtaattt agcctgcaac attaatggca
     7141 tatcacctat ttcatcgaga aacagcgtgc ccgtattcgc cgcctgaatc aaccctgttt
     7201 taccgttggc agaagcacca gtaaatgcgc ctttaacata accgaacagt tcgctttcca
     7261 gaagctgctc cggaatcgcg gcacagttga tagcaataaa gggtttattc cgtcttccgc
     7321 tcaacttatg gattgctcgg gcgacgactt ctttacccgt gccgctttca ccaaccacca
     7381 taacgctgga tgggctgggt gcaatacggc taatgagtcg ttttaattgc cgcataacac
     7441 ggcactcgcc aaccaattgt tcaatatgtg gttcatcagg tgcatttgct acagaaaaac
     7501 tggtatgcga ttggtgaaat gccattaaaa ataattgtcg gccctgaatg ttatgcaatt
     7561 gaccaatgat taattcactt ttatcgtccc acgaaacaat atgctgcata tgtccatgag
     7621 taaaattact ctcaaatgtt aatggtctga aacggatagg tttcccaata atattatttt
     7681 gtacaacacc aagtgttttt aaggcagtct gattaacaaa ctgaacccga ctttcatcat
     7741 caacaactaa tacgccctga tccatattat cgatcatggt cgcgaatatt ttactgatgt
     7801 tatctcctgg cccctgatcc tccagaagtt tcgaaacaaa aatggtggat atatggcgaa
     7861 cataatcaga aaattcgcgt aaattatcac tgatatgctc ttgttgctcg tgggtaacgg
     7921 caatcaaact tatcacccca acacaacgat cctgtaaaat gacaggcgta cccagaaatg
     7981 ctttttcgcg gcaattttct ttactatcgc aaccttcgca aaggggatcg aagcgagact
     8041 gtgtcacaac tttttcagtt ttcgtttcca ggacgtggcg gagcaggcgt gagttgccgc
     8101 tcaactggcg accaagaaac ttcccatacg cgcccgttcc ggcgacgcga cacaagtttt
     8161 catcaacgat ctcaacctca aactgcaaaa cgctggcaag cattctggca aaacgctgaa
     8221 ttgtcggttg aatttgcatc aatactgact gcgtagtagc aagctccata gctttacctt
     8281 ccagacttac ttaaaagtcg atcattgaag acgttgatgg ttcacagatc atgatgatat
     8341 taactcaggc gaaattggct ttgataaaaa cataagattt ttatcatttt ctaatgcaat
     8401 tatggaagag atatcacatt tctatatcaa tatgagaatt acggcggtga gtttatcaaa
     8461 ctgaagagag atagcctgcc cctttatctt atttctgata cttagcagaa aataaataac
     8521 gcgataaaaa aagccaaacg ttttcgtatt ttacaaacaa ccagaagctg gcatcaattt
     8581 gtgaacaacc ccacacatta tccgtcaaat tagtcttttg cagccgcgcg gataattctg
     8641 gcacacttat tgttagtccc aggtatagct atgaaaacac caatcacttt ggcaagtcac
     8701 agtgaaataa accactttgc ctgtcattcc actaccggga ctgtatgatg aaaactgtta
     8761 atgagctgat taaggatatc aattcgctga cctctcacct tcacgagaaa gattttttgt
     8821 taacgtggga acagacgcca gatgaactga aacaagtact ggacgttgcc gcagcattaa
     8881 aagcactgcg tgctgaaaac atctcaacca aagtctttaa tagtggatta ggtatttccg
     8941 tattccgcga caactccacc cgtacccgct tctcttatgc ttccgcgctt aacctgctcg
     9001 gccttgcaca acaagatctc gacgaaggca aatcacaaat cgctcacggc gaaaccgtgc
     9061 gtgaaaccgc caatatgatc tccttctgcg ccgacgctat tggtattcgc gacgatatgt
     9121 atctgggcgc aggcaacgcc tatatgcgtg aagttggcgc tgcacttgat gacggttaca
     9181 agcagggtgt actgccacag cgtccggctt tagtgaacct gcaatgcgat attgaccacc
     9241 cgactcagtc aatggctgac ctcgcgtggt tacgtgaaca ctttggttca ctggaaaacc
     9301 tgaaaggtaa aaaaatcgcc atgacctggg cctactctcc aagctatggc aaaccgctct
     9361 ctgtaccaca aggcatcatc ggtctgatga ctcgcttcgg tatggatgtc accttggccc
     9421 atccggaagg ctacgacctg atcccggatg tggttgaagt ggcgaaaaac aatgctaaag
     9481 cctccggtgg tagcttccgt caggtcacca gcatggaaga agccttcaaa gacgcagaca
     9541 tcgtttatcc gaagtcatgg gcaccttaca aagtgatgga agagcgtact gaattgctgc
     9601 gtgcgaacga ccacgaaggc ttaaaagcac tggaaaaaca gtgtctggca cagaacgcgc
     9661 aacacaaaga ctggcattgt actgaagaga tgatggaact gacccgtgat ggcgaagccc
     9721 tgtacatgca ctgcctgcca gctgatatca gcggcgtatc ctgcaaagaa ggtgaagtga
     9781 ctgaaggcgt attcgaaaaa taccgtatcg ctacctacaa agaagccagc tggaagcctt
     9841 atatcatcgc cgcgatgatc ctgtcccgta aatacgccaa accaggtgca ctgctcgagc
     9901 aactgctgaa agaagcgcaa gaacgcgtga aataacccct cgggccgatc tgctggtcgg
     9961 ccctctattt ccagaggcca aaaggatagg atatgtccgt tttctcattg aagattgata
    10021 tcgccgataa caaatttttc aacggcgaaa catcaccgct cttttcgcaa agccaggcca
    10081 aactggcgcg ccagttccac cagaaaatag ctggttatcg cccaacaccg ctttgtgcgc
    10141 tggacgatct cgcaaacctt tttggtgtga agaaaattct cgtcaaagac gaatcaaaac
    10201 gattcggtct gaacgccttc aaaatgcttg gcggtgcgta cgccatcgct caattattgt
    10261 gtgaaaaata tcatcttgat atcgaaacgc tgtcatttga gcacctgaaa aatgccatcg
    10321 gcgaaaaaat gactttcgcg accaccaccg acggcaacca cgggcgcggt gtggcgtggg
    10381 cagcacagca actcggacag aatgcggtga tttacatgcc gaaaggttct gctcaggaac
    10441 gcgttgacgc cattctgaac ctcggtgccg agtgcatcgt cacggatatg aactatgacg
    10501 ataccgttcg cctgaccatg caacacgcgc agcagcacgg ctgggaagtg gtacaggaca
    10561 cggcatggga aggttacacc aaaatcccaa cctggatcat gcaaggctac gcaaccctgg
    10621 cagatgaagc cgtcgagcaa atgcgtgaaa tgggcgtaac cccgacgcac gttctgctgc
    10681 aagccggtgt cggagcaatg gccggtggtg tgctgggtta tctggtcgac gtctacagcc
    10741 cgcaaaatct gcacagcatt attgttgaac ctgacaaagc tgactgtatt tatcgctccg
    10801 gcgtcaaagg cgacatcgtc aacgttggcg gtgatatggc caccatcatg gcgggcctgg
    10861 cctgtggcga acctaacccg ctgggctggg aaatcctacg taactgcgcc acccaattca
    10921 tctcctgcca ggacagcgtt gccgcattag gtatgcgcgt gctgggtaat ccgtacggca
    10981 acgacccgcg catcatctcc ggtgaatccg gcgctgtcgg tttgggcgtt ctcgcagcgg
    11041 ttcattatca cccgcaacat caaagcctga tggaaaaact ggcgctggac aaagatgccg
    11101 tagtgctggt tatcagcact gaaggcgaca ccgacgtgaa gcactaccgc gaagttgtct
    11161 gggaaggcaa acacgctgta gcaccttaat caccctattg gaactactcc ctgcaaaacg
    11221 gggagtaaaa aatctggaga aaaataatgg ctaagaatat tccattcaaa ctgattcttg
    11281 aaaaagcaaa agattaccag gcggacatga ctcgcttcct gcgcgacatg gttgctattc
    11341 ccagtgaaag ctgcgacgaa aaacgcgtag tacatcgtat taaagaagag atggaaaaag
    11401 tcggcttcga taaagttgaa atcgacccga tgggcaacgt tctcggttat atcggccacg
    11461 gcccgcgtct ggtggcaatg gacgctcata tcgataccgt cggcattggc aacatcaaaa
    11521 actgggactt cgatccgtac gaaggcatgg aaactgatga gctaatcggt ggtcgcggta
    11581 cttccgacca ggaaggcggc atggcatcta tggtttatgc cggtaaaatc attaaagacc
    11641 tcggtctgga agatgaatat accctgctgg ttaccggtac tgtgcaggaa gaagactgcg
    11701 acggtctgtg ctggcagtac attattgaac aatccggcat tcgcccggaa tttgtggtca
    11761 gtaccgaacc aaccgactgc cagatatacc gtggtcagcg cggtcgtatg gaaattcgta
    11821 ttgatgttca gggtgttagc tgccacggtt ctgcgccaga acgcggtgac aacgccattt
    11881 tcaaaatggg tccgattctt ggcgaattac aagaactctc ccaacgtctg ggttatgacg
    11941 aattcctcgg caaaggcacc ctcaccgttt ctgaaatctt cttcacatcc ccaagccgtt
    12001 gcgctgtagc agatagctgc gccgtctcta ttgaccgccg tctgacctgg ggcgaaacct
    12061 gggaaggcgc gctggacgaa atccgcgccc tgcctgcagt acagaaagct aacgcggttg
    12121 tttctatgta caactacgac cgtccgtcct ggactggcct ggtttaccca accgaatgct
    12181 acttcccgac ctggaaagtg gaagaagatc acttcaccgt taaagcactg gtgaatgcct
    12241 acgaagggct gtttggcaaa gcgccggttg ttgataagtg gaccttctca actaacggcg
    12301 tatctatcat gggccgtcac ggcattccgg tgatcggctt tggcccgggt aaagaacctg
    12361 aagcgcatgc acctaacgaa aaaacctgga aatctcacct ggtgacctgt gccgcgatgt
    12421 acgctgcaat cccgttaagc tggctggcaa cagagtaatt actcttcact tattcccctc
    12481 cggttcgccg gagggttttt ggagtttgct atgcgcgtat tgatcaaaaa cggcactgtc
    12541 gttaacgcag atggacaagc caaacaggat ttgctgattg aaagcgggat tgttcgccag
    12601 ttgggcaaca atatttcgcc gcagctcccg tatgaagaaa ttgatgccac tggctgttac
    12661 gttttccctg gcggcgtgga tgtccatacg catttcaata ttgatgtcgg catcgcgcgc
    12721 agttgtgatg attttttacc ggtacccgcg cagctgcgtg tggcggtaca acaaccatta
    12781 ttgaccatat gggatttggc ccaaacggct gtcggttacg ccatcaactg gaggtttatc
    12841 gtggttatgc cgcccataaa gcggtcatcg attacagctt tcacggtgtg atccagcaca
    12901 ttaatcacgc aatcctcgac gaaatcccga tgatggtcga ggaaggactg agcagtttta
    12961 aactctattt aacctatcaa tacaaactca acgatgacga ggttttgcag gcattacgcc
    13021 gcttgcatga atccggcgcg ctgaccaccg tgcacccgga aaatgatgcg gctatcgcca
    13081 gcaagcgggc ggaatttatc gccgcagggt taaccgcgcc gcgctatcat gccttgagtc
    13141 gccctctgga atgcgaagcg gaagccatcg cccgcatgat taacctggca caaattgccg
    13201 gtaacgcccc gctctatatc gtgcacctgt ctaacggctt aggtctggat tatctgcgtc
    13261 ttgcccgtgc gaatcaccag ccagtctggg ttgaaacctg cccacaatat ctcctgttgg
    13321 acgaacgcag ttacgataca gaagatggca tgaagttcat tcttagccca ccgctgcgta
    13381 acgtacgcga gcaggacaaa ctgtggtgtg gcatcagcga tggtgcgatt gacgtggtgg
    13441 cgaccgatca ctgcaccttc tcgatggctc aacgcctgca aatttctaaa ggcgatttca
    13501 gtcgctgccc aaatggctta cccggtgtgg aaaaccgcat gcagttactg ttttccagtg
    13561 gcgtgatgac gggacgtata acaccggaac gctttgttga attaaccagc gcaatgcccg
    13621 ccaggctgtt tggcctgtgg ccgcaaaaag gattattagc gcccggttcc gatggcgacg
    13681 tggtgattat cgacccacgt cagagccaac aaattcagca tcgccatctc cacgacaacg
    13741 ccgactactc gccatgggag ggttttacct gtcagggcgc gattgtcaga accttatccc
    13801 gtggtgaaac gattttctgt gacggcacct ttacaggcaa agccgggcga ggtcgtttcc
    13861 tgcgacgcaa accgtttgtc cctcccgtgc tctaaccact gtcagggatc tatacacaat
    13921 caagaatgat gagtgaggaa aaatgagtaa gaaaattgtt ctcgccctgg gcgggaatgc
    13981 gctgggcgat gacctggccg gacaaatgaa agcggtaaaa attacttctc aggcaattgt
    14041 tgatttaatt gcccaaggac atgaagttat cgtcactcat ggtaacgggc cgcaggtggg
    14101 catgattaac caagcctttg aagctgccgc aaaaactgaa gcacactcgc caatgctgcc
    14161 gatgtctgtc tgtgttgcct taagccaggg ttatattggt tacgatctac aaaacgcgtt
    14221 aagagaagaa ctgctttctc ggggcattaa taaacctgta gcaacgctgg ttactcaggt
    14281 cgaagtcgac gctaacgatc cggcattcct caacccgacc aagccgatcg gttcgttctt
    14341 taccgagcaa gaagcagagc aactgacaaa acagggttac actctgaaag aagatgctgg
    14401 tcgcggctat cgccgtgttg ttgcctcgcc aaaaccggtt gatatcattg aaaaagaaac
    14461 ggttaaagct ctggtagatg caggccaggt ggtgatcgcc gttggcggtg gtggtatccc
    14521 tgttattcgt gaaggtaacc atctgcgtgg tgccagcgcg gttatcgata aagactgggc
    14581 cagcgcccgt ttagcagaaa tgattgatgc cgatatgctg atcattctga ctgcggtaga
    14641 aaaagtggcc attaacttcg gtaaagagaa tgaacagtgg ctcgatcgcc tgtcattaag
    14701 tgatgctgaa cgctttatcg aagaaggtca ttttgcgaaa ggttctatgc tgcctaaagt
    14761 ggaagctgcc gcctcgtttg ctcgctctcg cgcaggtcgt gaagcactga ttaccgtatt
    14821 gagcaaagcg aaagaaggga ttgaaggcaa aaccggaacg gtgatttgtc agtaatttta
    14881 aggccgggtg ggtaatacca tccggccatt ttcatttaag ccacttccag cacttctttt
    14941 gtcgctttca cgccctgatg catcaacatc attaacgcct caagtacgcc gccgccaatc
    15001 gcccgggctt tatcagaaac gctggtgaaa tcagcggttt caccacgagg atcgatatca
    15061 ccaattttaa aaccaccaac cactgccagg ccgtcgttca acaagccacg caccatcccc
    15121 gtcaacggtg ctttaatttc atgctcacca atccaggcaa tcacatcgcc ctctttcacc
    15181 agatcgccta atttcacgtt ggatcgcata atgcctgcag caggagcacg gatcacccgt
    15241 cgggtggtat gccccataat attgccagga acatcggtat tctcctgcgc acaaccagag
    15301 taaatcacct gaccgagcca gtgcccgcga tttgtttcaa ttaccgcatg acaatccttc
    15361 cctgcagtaa agcccggccc aagcgcgatt gttactggtg ccatatctgc ccgcgttccc
    15421 aaattctgtt tcgccagaat agcgtccacc acgcaaagcg gtttcagttc atcaagcaat
    15481 gaacaggcgg gatctaccat cacagggatg aatccgcgtt cggtaagttt catcgcttcc
    15541 gcagagctgg ttgccaggcg agcagtgacg ccttcgaccg tcatttcgcc atcgaacacg
    15601 gcctgggcaa acgccacggt acaacgaatc actgtcggtt tttccacttc cagcatgatc
    15661 actttaaaac ccgcatgata cagacgtagc gccacaccac tggcgatatc tcccgcaccg
    15721 cgaatcacca ccagctggtg tccggaagga tgagaaggtt tcatcatcag cccacccggc
    15781 gcgttatttt tcacccataa tatttctgcc agcacgctga tggcgatctc ctgcggcgtt
    15841 tccgcaccta tgttgtaacc aacgggcgca tgtaaacggg caatatgttc ttcagccacg
    15901 cctttctcac gcaattgacg caggaaaagc tgaaccttgc ggcgacttgc cagcaacccc
    15961 aaccatgcaa tgggctgttc aatgagtttg tcgagggctt cacgatcctg attattcgtg
    16021 gcaatcagga caaaattatc agggcgaata tccagttctt ccactgccgc accaaacgac
    16081 tcagcatgga gaagcgtggt tgatggtggg aatagttcag gattgaggct ttcgcgataa
    16141 atatcggcaa cggcaatatc aaatcctaat agcgccgcac tctgggctat cgcccggttg
    16201 acatgccccg cgccaatcaa caccagacgt ggacgcatac catggacgct gataaatact
    16261 gacattgcac ctccacaatc tgacccgaca gcatccgcac cgttacgagc catacgtcca
    16321 tggaataatc gcggcttacg ttcctgcaat gcctgaagtg actcttcaat caccttccgc
    16381 tcaaccattc cgccaccaat tgtaccgacg atagaaccat cggcgcgcac taacatttgt
    16441 gcagaatggc ggggagtcga gcctcggcta tcaacaattt gcgccatcgc aaacggacaa
    16501 ttttgctctt cgagttttgc agcctctgtg aaaatattca taccaacctc aatggatatc
    16561 ctttcagtaa cccggaatac ccgggccgct aattcacaaa tctgcgcgcg attgcaggat
    16621 gttcttgtat atcgcccagc cagattgcct ctacgtcgtg ttgttgcagc ggttgaagga
    16681 gctcgctttg tgcaatcgca ttctcacatt gagaaaaacg gttaatgaac cagactcgcc
    16741 gacaaccttg cggtacgttt ttaaacgccc cctgagggtg gcgaaccagc gcaacgagat
    16801 cgctcagttg caaggttgca tcaggtgtta acccggtaat atcagcaaac tgcgaccagc
    16861 gatggacatt ttctgtgctc actttcgcgc ccaaagtatg tcctcccatc acagcaatca
    16921 cgcagcaact gcttttgggt atgcaaggtt cgtgctcatc aggcgctttt aacggcattc
    16981 cacgcgagcc atctgcttca atgagaatta cgtcacactc tggtcgttgc accagtgcat
    17041 caatcgcttc tggcgtaaat ccctgcactt ttccctggtt cgctttccag ctgtgaaaac
    17101 aaaatgaaat gggggatgta agagacgcat gaggaagcat ggcgggatca cgacagaaaa
    17161 ccacgggcca gtgagacgtt ggcataaaca tatgtgtcgt cgtcgtgatt aacacacgcc
    17221 tgccgctagc ctggagcagt tctgccagcc aaaaaagcaa gctggttttc cccccagcac
    17281 caacaacaga aatcacagtg ggacgtttct gcgcacctaa atcaatgact aatgccgatg
    17341 ggtcaactat acttttcacg agtctttatg acctctctgg gataaattat ccccaactta
    17401 atccatcagg aagtaacgca attatcaggc gttattagcc cctataaata atggaaccac
    17461 tatgtcagcc atcgactgta taattaccgc tgccggatta tcatcaagaa tggggcaatg
    17521 gaaaatgatg ttcccctggg aacagggaac aattcttgat acaagtatca aaaatgcgtt
    17581 gcagttttgt agccgaatta ttttagtcac cggctatcgt ggtaatgaac tgcacgaacg
    17641 ttatgcgaac cagagcaata tcactattat tcacaaccca gattatgcgc agggtttact
    17701 aacatccgtc aaggccgcag tacccgcagt gcaaacagaa cattgttttc tcacccacgg
    17761 tgacatgcca accctcacca tcgatatttt tagaaaaatc tggtcgttac gaaatgatgg
    17821 cgcaatactg ccactccata atggcatccc cggccatccg attttagtgt caaaaccatg
    17881 cctgatgcag gcaatccagc gacccaatgt caccaatatg cgtcaggcac tccttatggg
    17941 agatcattat tccgtcgaaa tagaaaatgc agaaataatt ttagatattg atactccgga
    18001 cgactttatt acagcgcaaa aaaggtatac taaaatttag aaaatgtagt taaacgattg
    18061 cgttcaaata tttaatctct ccagcactct ttatcataat gataaggaat cactgatttt
    18121 gagaaaagag aaaaagtgat aaatgggctg acaataataa ttttcaggag tgattttaat
    18181 tctcatttgg cacctgtgag ttcattcaca aaaagccttt aagtttcgag ccacctcaca
    18241 tttttttata tttcccgcca aacctggcaa gagtggtgcg attgttgctc tatcccccta
    18301 aaccaccgga tttctcaaca ccggtcactc aatgatatct gtataagcta aggagagggt
    18361 tatgggggat attatgcgtc ccattccgtt tgaggaactt ttgacgcgca tatttgatga
    18421 ataccaacaa caacgctcaa tctttggtat tcccgagcaa cagttttact cacctgtaaa
    18481 aggtaaaact gttagcgtct tcggtgaaac ctgtgccact cccgtcggcc ctgccgctgg
    18541 cccgcacacg cagctcgcgc aaaatattgt cacttcctgg ctgactggcg gacgcttcat
    18601 cgaactaaaa accgtccaaa ttcttgaccg cctggagctg gaaaagccct gtattgatgc
    18661 cgaagacgaa tgctttaaca ccgaatggtc taccgaattt accttgctta aagcctggga
    18721 tgaatacctc aaagcctggt ttgccctgca ccttctcgaa gcgatgttac agccttctga
    18781 ttccggtaaa tcgttcatct ttaatatgag catcggttac aacctcgaag gtattaagca
    18841 accgccgatg caacagttca tcgacaatat gatggacgca tctgaccatc cgaaattcgc
    18901 tcaatatcgc gatacgctga ataaattact ccaggatgac gcatttttag cccgccacgg
    18961 attgcaggaa aaacgcgaaa acttgcaagc cttacccgct cgcatcccca ccagtatggt
    19021 gcagggcgtc cccctctcca ccatgcacgg ctgtcctccg catgaaatcg aagccatttg
    19081 ccgctacatg ctggaagaaa aagggctcaa cacctttgtc aaacttaacc cgaccttact
    19141 ggggtacgcg cgtgttcgtg agatcctcga tgtctgcggt ttcggttaca taggcttaaa
    19201 agaagagtca tttgatcacg acctcaagct gacgcaagca ctggaaatgc tggagcgcct
    19261 gatggtactg gcaaaagaaa aatcactcgg ctttggcgta aaactgacta acactctcgg
    19321 caccatcaac aataaaggcg cactgcctgg tgaagagatg tatatgtcag gccgtgcgct
    19381 gttcccgctc tccatcaatg ttgcagcagt tctctctcgc gcctttgacg gcaaactgcc
    19441 catttcttat tccggtggtg ccagtcagct gactatccgc gatatttttg atacaggtat
    19501 tcgccctatt actatggcaa ccgacctgct gaaacctggc ggctatctgc gcttaagtgc
    19561 ctgcatgcgc gagctggaag gctccgacgc ctggggactt gaccatgttg acgtcgaacg
    19621 actgaacaga ctggcagcag acgcgttaac catggaatac acccagaaac actggaagcc
    19681 agaagagcgt attgaagtgg cagaagacct gccgctgacc gattgctacg ttgccccctg
    19741 tgttactgcc tgcgctatca agcaagatat tccggaatac atccgtctgc ttggcgaaca
    19801 ccgctatgcc gacgcgctgg aactcatcta ccaacgcaac gccctacctg ccattaccgg
    19861 tcatatttgc gatcaccagt gccaatacaa ctgtacccgc ctggattacg acagtgcgct
    19921 gaatatccgc gaactgaaaa aagtcgcgct ggaaaaaggt tgggatgaat ataagcaacg
    19981 ctggcacaaa cccgccggtt ctggttcacg ccatccggtt gccgtgattg gtgcaggtcc
    20041 ggcgggtctg gcagcaggtt acttccttgc cagagcgggc catccggtta cgctgtttga
    20101 acgcgaagcc aatgctggcg gcgtggtgaa aaatatcatt cctcagtt
//
LOCUS       DAJVYM010000070        20006 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVYM010000070 DAJVYM010000000
VERSION     DAJVYM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20006
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.070"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..497
                     /gene="radC"
                     /locus_tag="OQS60_002289"
     CDS             <1..497
                     /gene="radC"
                     /locus_tag="OQS60_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS2388023.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            714..950
                     /gene="rpmB"
                     /locus_tag="OQS60_002290"
     CDS             714..950
                     /gene="rpmB"
                     /locus_tag="OQS60_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS2388024.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            971..1138
                     /gene="rpmG"
                     /locus_tag="OQS60_002291"
     CDS             971..1138
                     /gene="rpmG"
                     /locus_tag="OQS60_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS2388025.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1236..2045
                     /gene="mutM"
                     /locus_tag="OQS60_002292"
     CDS             1236..2045
                     /gene="mutM"
                     /locus_tag="OQS60_002292"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS2388026.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2084..2563)
                     /gene="coaD"
                     /locus_tag="OQS60_002293"
     CDS             complement(2084..2563)
                     /gene="coaD"
                     /locus_tag="OQS60_002293"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS2388027.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2571..3848)
                     /gene="waaA"
                     /locus_tag="OQS60_002294"
     CDS             complement(2571..3848)
                     /gene="waaA"
                     /locus_tag="OQS60_002294"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS2388028.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4261..5319
                     /gene="rfaQ"
                     /locus_tag="OQS60_002295"
     CDS             4261..5319
                     /gene="rfaQ"
                     /locus_tag="OQS60_002295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS2388029.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5316..6440
                     /locus_tag="OQS60_002296"
     CDS             5316..6440
                     /locus_tag="OQS60_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS2388030.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6433..7230
                     /gene="rfaP"
                     /locus_tag="OQS60_002297"
     CDS             6433..7230
                     /gene="rfaP"
                     /locus_tag="OQS60_002297"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS2388031.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7246..8262
                     /gene="waaO"
                     /locus_tag="OQS60_002298"
                     /gene_synonym="rfaI"
     CDS             7246..8262
                     /gene="waaO"
                     /locus_tag="OQS60_002298"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS2388032.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8279..9274
                     /locus_tag="OQS60_002299"
     CDS             8279..9274
                     /locus_tag="OQS60_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS2388033.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9284..9976
                     /gene="rfaY"
                     /locus_tag="OQS60_002300"
     CDS             9284..9976
                     /gene="rfaY"
                     /locus_tag="OQS60_002300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS2388034.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10002..11027
                     /locus_tag="OQS60_002301"
     CDS             10002..11027
                     /locus_tag="OQS60_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS2388035.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11112..12095
                     /locus_tag="OQS60_002302"
     CDS             11112..12095
                     /locus_tag="OQS60_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS2388036.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12141..13394
                     /locus_tag="OQS60_002303"
     CDS             12141..13394
                     /locus_tag="OQS60_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS2388037.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13464..14435)
                     /gene="rfaC"
                     /locus_tag="OQS60_002304"
     CDS             complement(13464..14435)
                     /gene="rfaC"
                     /locus_tag="OQS60_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS2388038.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14439..15485)
                     /gene="rfaF"
                     /locus_tag="OQS60_002305"
     CDS             complement(14439..15485)
                     /gene="rfaF"
                     /locus_tag="OQS60_002305"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS2388039.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15495..16427)
                     /gene="rfaD"
                     /locus_tag="OQS60_002306"
     CDS             complement(15495..16427)
                     /gene="rfaD"
                     /locus_tag="OQS60_002306"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS2388040.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16641..17837
                     /gene="kbl"
                     /locus_tag="OQS60_002307"
     CDS             16641..17837
                     /gene="kbl"
                     /locus_tag="OQS60_002307"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS2388041.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17847..18872
                     /gene="tdh"
                     /locus_tag="OQS60_002308"
     CDS             17847..18872
                     /gene="tdh"
                     /locus_tag="OQS60_002308"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS2388042.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19111..>20006
                     /gene="waaH"
                     /locus_tag="OQS60_002309"
     CDS             19111..>20006
                     /gene="waaH"
                     /locus_tag="OQS60_002309"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCS2388043.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5398 a         4362 c         4725 g         5521 t
ORIGIN      
        1 tgttaacctc tgaatatgaa caatttagtg gcgttcatgg aattggcgta gcgaaatttg
       61 cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag
      121 aaagcccttt actcagcccg gagatgacgc gggaattttt acaaagccag ctcacgggtg
      181 aggagcggga gatctttatg gtgatctttc tcgactcaca acaccgggtt ataacgcata
      241 gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccggg
      301 aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg
      361 ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca
      421 tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg
      481 aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact
      541 tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt
      601 tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga
      661 ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc
      721 gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga
      781 acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg
      841 aaaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag
      901 gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag
      961 gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac
     1021 tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa
     1081 aaaattcgat ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt
     1141 ctcgctttga tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc
     1201 catattgatt gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa
     1261 ccagccgtcg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc
     1321 gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag
     1381 tgcttagcgt gcagcggcgg gctaaatatc tgctgctgga gctgcctgag ggctggatta
     1441 tcattcattt ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa
     1501 agcatgacca tgtggatttg gtgatgagca acggcaaagt gctgcgctac accgatccgc
     1561 gccgctttgg tgcctggctg tggaccaaag agctggaagg gcacaacgtg ctggctcatc
     1621 ttggaccgga gccgcttagc gacgatttca atggtgagta tctgcatcag aagtgcgcga
     1681 agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga
     1741 atatctatgc cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat
     1801 cactgtcgct ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt
     1861 cgattgagca gggtggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct
     1921 atttcgcgca ggaattgcag gtttacggac gaaaaggtga gccgtgtcgg gtgtgcggta
     1981 cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga
     2041 agtaattcat acgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca
     2101 ccagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca
     2161 cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac
     2221 tttccagttc cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat
     2281 ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg
     2341 ccatcaaatc actaaaccca accacttcca cgttccccag atgcgcggtt gcctgctgtg
     2401 tcagcgccac acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg
     2461 ccagaataac gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg
     2521 taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt
     2581 ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga
     2641 tacagtactt caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa
     2701 gaggaaacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc
     2761 tccagccgcg cgcaaatgtc tttgaaatta aaagtatgcg gccccatcaa taccggaata
     2821 gcgtgtgcgg cagcttccag aggattatgc cctccacgtt caaccagtga accgccaaca
     2881 aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca
     2941 acctgcgtgc tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc
     3001 tggcggacaa ggttaatcgc atccgggaag cgttctggat gacggggtac caggatgagc
     3061 aataaattcg ggaattgctg taataatgcc tgatgtgcgg cgatcaccac actctcttca
     3121 ccttcgtgag tgctggtggc aatccatacc gggcggtgtg gtgcccactg gcggcgcagc
     3181 gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta
     3241 acggttacct ggttattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt
     3301 tgcgcagcaa tcagcgtaat acgacgcagt aagcggcgga cgaatttacc cagtttggca
     3361 taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt
     3421 ttatgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagtttg
     3481 gggtcgactt tattcaggaa acggttgagc gcatcgggca ggtcatacgg cagataaacg
     3541 tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt
     3601 gtaacggtaa tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc
     3661 gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg
     3721 cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc
     3781 acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat
     3841 tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg
     3901 ctttaccatt acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg
     3961 aacatctata ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga
     4021 aggaatcgca gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc
     4081 tttattgaaa atcaacaatg tgcttaaaaa gcattcgggt cggattgccg cgaaagaagt
     4141 gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg
     4201 gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt
     4261 gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta
     4321 ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg
     4381 ctgctttatc aggacaccac cccgatcctg tctgaaaatc cagagattaa cgcgctctac
     4441 ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc
     4501 aaggtattac gtgccaataa gtatgacctt atcgtcaatc ttaccgatca atggatggtt
     4561 gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg
     4621 cagtctgctt tttggcgtaa aagtttcacc catttagtgc cgttgcaggg tggaaatgtg
     4681 gtggaaagta acttatccgt gctgacacca ttgggacttg attcgttggt gaagcagaca
     4741 accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt
     4801 gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac
     4861 aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg
     4921 acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag
     4981 acgccaccag taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc
     5041 gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt
     5101 aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg tccttggtca
     5161 aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt
     5221 gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat
     5281 aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata
     5341 aatattttcc ctttggcggt ttgcagcgcg attttatgcg tattgctcag acagtcgccg
     5401 cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat
     5461 ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt
     5521 ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca
     5581 aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtcgcgc
     5641 aggaaaaagg ctttttctat cacctgacgt cacgttatcg ccattatgcc gcctttgagc
     5701 gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat aagcaaatcg
     5761 ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga
     5821 tttatcctga tcgtaaatat agccagcagc cagctaatag ccgtgaaatc ttccgtaaga
     5881 agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta
     5941 aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca
     6001 cattgctata tgttgttgga caggataagc cgcgaaaatt tgaggcgctg gcagaaaaac
     6061 gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg gaattaatgg
     6121 cggcggcgga tttattactg catcctgcct accaggaagc agcgggaatt gtgctactgg
     6181 aagcgattac tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata
     6241 ttgtcgacgc caattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg
     6301 agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc
     6361 gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa
     6421 cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg cggtaaagat
     6481 ccttttgagg aagttaaaac cttgcagggt gaggtttttc gtgaactgga aactcgccgc
     6541 actctgcgat ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc
     6601 ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg cgcagaccgc
     6661 gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca
     6721 tttggcgaaa aaggtattaa tccgctgagc agaacctcgt ttattattac cgaagatctg
     6781 accccaacca tcagtctgga agattactgt gctgactggg cgaccaaccc accagatgtt
     6841 cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgtg
     6901 ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt
     6961 aaggaagagg agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc
     7021 gttccacgtc gttggcgcga taaagatctt attgggcttt atttttcttc gatgaatatc
     7081 ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa
     7141 gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc
     7201 agggaaagaa cgattcgaaa atcgttgtaa tttccggaga gaaatatgag tgcccactat
     7261 tttaatccac aagagatgat caataagaca atcatcttcg atgaaaggcc agcggcgtca
     7321 gtggcatcat cattccatgt tgcttatggc attgataaaa actttctttt tggttgtggt
     7381 gtttcaatca cgtcagtttt gttacataac aacgacgtga gttttgtttt ccacgttttt
     7441 attgatgata tccctgaagc cgatatccag cgtttagccc aattggcgaa aagctatcgt
     7501 acctgtatcc agatccatct ggtaaattgt gaacggctta aggcattacc gacgaccaaa
     7561 aattggtcta ttgccatgta tttccgtttt gtaattgcag attactttat tgatcaacaa
     7621 gataagatcc tttacctgga tgctgatatc gcctgtcagg gaaacttaaa gccgctgata
     7681 acaatggatc ttgccaataa cgttgctgct gttgttactg aacgcgatgc taactggtgg
     7741 tcgttacggg gtcaaagtct gcagtgtaat gaacttgaaa agggttactt taattcaggt
     7801 gtcctgttaa ttaatacact agcgtgggcg caggagtccg tttctgctaa agcgatgtcg
     7861 atgcttgctg ataaagccgt cgtttcccgt ttaacctata tggatcaaga tatacttaat
     7921 cttatcctgt cagggaaagt taaattcatt gatgctaaat acaatactca atttagttta
     7981 aattatgaat taaaaaaatc atttgtttgt ccaattaatg atgaaaccgt attaattcat
     8041 tatgtcggcc cgacaaaacc ctggcattac tgggccggtt atccaagtgc gcaacctttt
     8101 atcaacgcca aagaagcatc gccctggaaa aatgaaccgt ttatgcggcc aattaattca
     8161 aactatgctc gttattgcgc caagcataat tttaaacaaa atgaaccaat taacgggata
     8221 atgaattata tttattattt ttatttaaag ataataaaat gatattgaga aaagttaaat
     8281 gaatgaattt ataaaagaac ggttttcgta tttagcagat aataaaaaag aaaacgcccc
     8341 agaactaaat gtttcctacg gtatcgataa gaattttttg tatggtgctg gcgtttcaat
     8401 ttcttccgtt ttgattaata attcagatat taattttgtc tttcatgttt tcactgatta
     8461 tgtggatgat gattatttaa agtcatttaa tgaaacagca aaacaattta atacctcaat
     8521 tattgtatat ttaattgacc caaaatactt tgctgatctg ccgacgtcac agttttggtc
     8581 gtacgcgaca tacttcaggg tattgtcctt tgaatatctg agtgaaagta tttccacact
     8641 gctgtatctg gatgccgatg tcgtttgtaa aggaagtctg aagcctctca cagaaattat
     8701 atttaaagat gagtttgctg cggtcattcc tgacaatgtt agtactcagg cggcatgtgc
     8761 aaaacgcctc aacattcccg agatgaatgg acgttatttc aatgcaggcg ttatctatgt
     8821 caatattaaa aaatggcatg aagcaaattt gacaccgtat ttactcacgc ttttacgagg
     8881 ggaaactaaa tatggctctc ttaaatattt agatcaggat gcgttgaata tcgcatttaa
     8941 tatgaataat atctacctcg cgaaggattt tgatactatt tataccctga aaaacgaact
     9001 tcatgatcgt agtcatcgaa agtatcagca aaccattacc gataaaacag tattgattca
     9061 ctatacaggg ataactaaac catggcatag ctgggctggt tatccgtctg catcatactt
     9121 taatatcgcg cgtgaacaat ctccctggaa gaaatatcct cttaaagagg cgcggactgt
     9181 tgcagaaatg cagaaacaat ataagcatct gtttgcccat ggtgagtata ttaaaggcat
     9241 aacttcatta attaagtaca agcttaagaa ataaattact cttatgatta caagtatacg
     9301 ctatcgcggc ttctcatttt attacaaaga taatgataat aaatataaag aaatctttga
     9361 tgaaatactg gcctataatt ttaaaacagt aaaagtatta cgtaatatta atgataccaa
     9421 agtatcattg attgatacga aatattgtcg ctatgttttt aaagtctttg ccccgaaaac
     9481 gaaaaggaat gaacgttttt taaaatcttt tgtaaaaggt gactactatc agaatttgat
     9541 cgttgaaacc gatcgtgtca gaagtgcagg tcttactttt cctaatgatt tttattttct
     9601 ggctgagcgt aaaattttca actatgccag tgtttttatc atgcttatcg agtatgtcga
     9661 gggcgtagag ctcaatgata tgccgatcat tccagaagat gttaaagcag aaattaaggc
     9721 ttcaatggaa aaattgcatg ccttaaatat gctatcgggc gacccacacc ggggtaactt
     9781 tatcgttagt aaagatggtg tcaggataat tgacctatcc ggtaaaagct gtacggcaga
     9841 gcgtaaagct cgtgatcgtc tggccatgga acgtcacctg ggtattgcca acgaaataaa
     9901 agattatggc tactattcag taatctacag aaccaaacta cgcaaattta ttaaaaaatt
     9961 aaaaggcaaa gcgtaaacca cacagtcaaa acggaaccaa catggattta ttagctgaga
    10021 gtattactga agtcgctgtc tctggggaaa ttgctaacac cgatcgtgtg ttaaatatcg
    10081 cttacggtat tgaccgtaac tttttatttg gtgcggcagt atctatgcaa tcagttgtta
    10141 tgcataatcc ggaccttgcg gttaagtttc atctctttac cgactacatt gatgaagatt
    10201 atctacaacg tgttaatgct tttaccagca aaaatgctaa cgttgaagta agaatttata
    10261 aagtctccag tgcctttatt gatatcttcc ccagcctgaa acagtggtct tatgcaacat
    10321 tcttccgttt agttgcgttc cagtatctga gtgaaactat tgaaaatctg ttatatatcg
    10381 atgctgatgt catctgtaaa ggctcattag ctggattgct tgatattaat tttgatgggg
    10441 ataagtttgc agctgttatt aaagatgtgc cttttatgca ggaaaaacca gcgaagcgtc
    10501 tggctataga gggacttcca gggaattatt tcaacgccgg tgtagtatat ctgcagcttg
    10561 aagcatgggc gaaaaatgat tttatgaata aagccattgc tatgctggca agtgacccgc
    10621 agcacacgaa gtataaatgc cttgatcagg atattttaaa tattctgttc tttggtcatt
    10681 gtatttttat tagcggcgat tatgattgct tttatggcat cgattatgag ttaaaaaata
    10741 aaagcgatga agattataaa aagaccatta ccgatgatac taagctgatt cattatgttg
    10801 gcgtaacgaa gccctggaac gactggacga attatccctg ccagaagtat tttaatgagg
    10861 cttatcaggc ttcttgctgg aatgatgtgg cgtttattcc agccacgaat gaaaagcagt
    10921 atcaagtgaa atatcaacat gcaaagaaaa atggtgatac gtttaacgct tttatttact
    10981 ttattaaatt taaattaaat aagtataaaa gaaaactatt tggataataa taaagatcat
    11041 ggatgtctga gttacaataa taattaagga tgagtactat catattttat tagttcaggc
    11101 aagagaacac gatgagcaat gattaccctt tagtatccat aataataccg acgtataatt
    11161 catctgatta cattactgaa actctaacga aattagaaaa acaaacttac ccaaattttg
    11221 aaattgttat tgttaatgat ggttctaaag ataacacatc aaacgttttg agagagtatg
    11281 ggttaaccca ctctcgatta attattatca ataaagaaaa tggcggtgtt tcgtctgcca
    11341 ggaatacagg tatccgcaag gcgcaaggac agtttatatg ttttatggat gatgatgatg
    11401 agatagatcc taactatctg ctgaagatgt ataccagaca acatgagacg ggaggggatg
    11461 cgatttattg tgggctttat ggccattata taaaagatgg tgttacttac tcacctataa
    11521 atacagagtt taatgaagga tctttacttt tcgacttttt ttataaaaag gttagattcc
    11581 atatagggtg cttgtttata agaaaacaac ttctggaaga gaataatctt ttttttgatg
    11641 aagatttacg actaggagaa gatctggatt ttatctatcg actgctaatt acatgcgata
    11701 tgtatgcggt tccatattat atgtataagc ataactatag agaaaattcc ttaatgaact
    11761 catgtagaac catcactcat tatcgacatg agtcatttgc gcacgaaaga atctactctt
    11821 ctgtgatgca gttatacaaa ggtaacagga aagaagaaat tcatacatta ttgagtcaaa
    11881 atagagctta tcataaaact cgttatttat ggaatgttct acttaatggt gattttgaac
    11941 tattaaatca attagttgaa agcaatgaaa aagaattgaa tgattgtaat ctttctggca
    12001 agagagataa aagacgagca aaaatattag catcaaaaaa ttatattctc tggaggatgg
    12061 taagactggt aaataggaaa aagaataaac gttagatctt gcattaaata atccgtcctc
    12121 gtagcatagg ttgaaactat atgtcatttt gttggaatga aattaattct ggtataaagt
    12181 ctttaattct catattatgt attttttctt tgatgacttt gtctttatgg gatgatgttg
    12241 caacaaagtt tcttcatgca gctggaatta tatctgcatt gtattttcta ataacaccaa
    12301 aaaaaacaat aactaacaat cctactttgt taattttcat ctcattatgt cttttgggaa
    12361 tcataaatat catctggtat tcacattata aagtttcagg ctctgtttat accaatgcat
    12421 atcgtggccc aatggaaact gggaaaattg ccttgtgtag tgcttttatt ttcttggttc
    12481 tttttgctaa aaatgagttg agaacaaaaa taaaatttgg gaaactaatt ctgttcgcat
    12541 ccctggcaac gcagttactt ttttttgcgc atgccatgtg gcaacatttc tatttaaacg
    12601 ttgaccgtgt tgcattatca gcttcccacg ctacaacagc aggctacatc atccttttcc
    12661 cttctttact ggcatcaatt ctcattttaa aatccgactt tagacataaa acaacattat
    12721 atacaattaa cttcatgctt agcttatgtg ctgtcatagt aactgaaacg cgtgcagcca
    12781 tattagtgtt tccattcttt gcgttactat taatcgtaat ggatagttat attaataagc
    12841 gaattaatta taagttatat tgttttattg cgattgcatt attagcaggt gtattttctt
    12901 ttaaagatac attgcttatg agaatgaata acttaaataa cgatttagtt aattattcgc
    12961 atgataacac cagaacttca gtcggtgccc gtctggcaat gtatgaagtt ggcttaaaaa
    13021 catattctcc aataggacaa tcactggaaa aacgagcgga aaaaatacat gaactagaag
    13081 aaaaagagcc tagattgagt ggcgctttac cctatataga ttctcatttg cataacgatc
    13141 tcatagatac gttatcaacg cgtggtattc ctggagttgt attaacaatt ttagcctttt
    13201 cagcaatact catatatgcc ttaagaactg ctaaagaacc ttatatttta atcttgcttt
    13261 tttcactact ggtagtaggc ctaagtgatg taatactctt ttctaaaccg gttccgactg
    13321 ctgtgtttgt caccataata ttgctttgtg cttattttaa agcacaatca gaccaatgtt
    13381 tattagataa ataatgaata tgtgaaataa aatcagcaag gaggctgatt ttatttcaaa
    13441 attatctatt aaggggttgt aatttaaatc atagcaacgt tttcttcaat gaatcgctta
    13501 acagcatttg ctgtcaattg agacaactca ttccccggag ccctacaaac catctgattc
    13561 ttcccatatc caccaattaa tcccggatcg gttggtccat aaaccgtgat attgggtcta
    13621 tccagtgccg ccgttaaatg gcttaacccc gtatccaccg acactacaaa tttagccccg
    13681 gccagcacgc gggcaacgcc ttccagactc atcttcggca atacttcaac ataagcaaat
    13741 ccttccgcca gtcgtttcgc ccgctcttcc tcatgcggcg cgccccacgg aagtttaatc
    13801 cgtattcctg aatcagccag taaaccaatc aattctcgcc agtgttcttc cggccagtgt
    13861 ttatcatcac gggtcgtcgc atgaagaaat acggcatatt cgccagcatc tgtaggcagg
    13921 ttcgtcagga aatgctgtgc aatagcataa tcgccctggg tttgcggttt gctatagccc
    13981 agacttttgg caaacagttc gcgggtgcgt tctacggcgt gctgctgttt tgcaatatga
    14041 tgcttacgat tgtaaaacag actggctaaa ggttcgcgag cggtttgcca gtccatgcca
    14101 tgctttacgc catgcgccag acgcgtcacc agcgccgcgc tttttaccag cccctgagcg
    14161 tcgataactg cgtcatagtt ctctgcttgt agcgcttcac gaaacgcttt gcgttccgct
    14221 tttatggggg ccgagaacca ggctttacgc cagcgacgta ttgccacagg aataactcgc
    14281 tcaacggcgg cgtgccagga aggaatctgt gcgaaccctt cttccaccac ccagtcaaac
    14341 ttaatccctg ggattgcctg ctgggcatca gtgagtgcgg gcaacgtatg gagaacatcg
    14401 cccatcgacg atgttttaac gatcaaaacc cgcatccgtc aggcttcctc ttgtaacaat
    14461 agcgcgttga gttcttccag tacgcgctgg ggagtaatgt cgattaagct ctggtgataa
    14521 ccctccgcag cgtcaccttt acgcactttg tgatagccgg taatcaggcg gatcacgcgc
    14581 gctttatggg atagcggcgg tgtgaagtcc gggctactcg gaccatacag ggcaaccagc
    14641 ggacgattga gcgccgccgc aacgtgcatc aggccagaat cgttagtgac aatggcttta
    14701 caggctgcaa tcaggataac cgcttgatca agctgtgttt cccccgccag gttccgacac
    14761 catgcctgct gctcggtatt caaagcggca agaatctcat tgcccgcttc atgatctttc
    14821 gccgagccaa acagaaccac ctgataacct tcatcaatca gctgctttgc cagttccgca
    14881 tagtggtagt gtggccagcg ttttgccgga ccaaactccg cacccgggca aaaaccaatc
    14941 atcggacgtt ctgatgaaag cgaaaattga ttacaggtat atgatttttc accttcgctc
    15001 acctgcaatt gcggccataa caacggtttt ggtagatctt gtgcggtacg catcacgcct
    15061 ttgtcatagg ccagcgcgac atagcgttcc accattagcg gccaggcttc tttatcgagc
    15121 acgcgtaaat cgttgagtaa accgtagcgc atctcgccgc gccagccggt gcgatgagga
    15181 atacccgcga agaaaggcac taatgcagat ttgaaggagt tgggtaagac gtaggcgcgg
    15241 tcgtagcgct tttcacgcag gctatgaccc agtttgcggc gttcgccgat ttccagcgct
    15301 ccgtgaccga gaggcatagg aatagcttcg ttaacttccg gcatccgcga taataatgga
    15361 cggcaccatg ccggtgccat cacatcgatt atcgcctggg gatagcgcgc ctggagcgtg
    15421 cgatagagac tttgcgacat catcatgtcg ccaacccaag acgggccgat caccagtatt
    15481 ttcatgcaga gctcttatgc gtcgcgattc agccaggcca tgtattccgt tacaccttca
    15541 gcaacggttt tgaacggttt gtcgtaaccc gccgcgcgca gatttgtcag atctgcctga
    15601 gtgaacgcct ggtagcggcc tttcagctta tccgggaacg gaatgtattc gatctggcct
    15661 ttcttgtgat aagccagcgt agcatcagct acagcctgga aggattccgc acgaccagta
    15721 ccgaggttga agatgccgga aacgccattt tccaggaacc acagattcac atcagccacg
    15781 tcgcccacat agacgaagtc gcgtttgaag ttctcgctac cttcaaacag cttcggtgat
    15841 tcaccgttgt taagctgggt gttgagatgg aaagcgacgc tcgccatgct gcctttatgg
    15901 ccttcacgcg gtccataaac gttgaaatag cggaagccaa caatctgcga gttagcttca
    15961 ggcaggattt ggcgaacata ttcatcaaac aggaattttg agtaaccgta gacgttcaac
    16021 ggtttttcgt actcgcggga ttcaataaag tcggaggtgc gtccgccgta ggtggctgcg
    16081 gaagaggcat acaggaacgg gatttcgcgc tccagacagt agtgcagcag ctctttggag
    16141 tattgatagt tgttatccat catatacttg ccatcccact cggtggtgga agagcacgcg
    16201 ccttcgtgga aaatcgcttc gacatcgccg aactcttcgc cagccataat ctggatcagg
    16261 aagtcttcct tatccatata gtccgcgata tccagatcca ccaggttcac aaacttggtg
    16321 ccgtctttca ggttgtccac caccagaata tcggtgatgc ctttatcatt cagggcctta
    16381 acgatgttgc tgccgataaa gcccgcgccg ccggtaacga tgatcataac tgtaaccttc
    16441 gaattatgga gtcagagaca atctcagaca cgaatacttc tatcatatca ctacacggcg
    16501 ttggcttcag ccattcgcca aaagtgagat tgatggacgt tatttatcct gaattttgca
    16561 gaagtgttaa cgcgtcatct cgtcgcgacc tataagtttg ggtaatatgt gctggaattt
    16621 gccctgtctg gagaatcgca atgcgtggag aattttatca gcagttaacc aacgatctgg
    16681 aaaccgcacg ggcggaaggg ttgtttaaag aagagcgcat tattacgtct gcgcagcaag
    16741 cagatatcac tgtggctgat ggaagccacg tcattaactt ttgtgccaat aactatctcg
    16801 ggctggcgaa ttatccggat ctgattgcgg cggcaaaggc gggaatggat tctcacggtt
    16861 tcggcatggc ttcggtgcgt tttatttgcg gcactcagga cagccataaa gagcttgaac
    16921 aaaaactggc ggcattcctg gggatggaag atgcgattct ctactcttcc tgctttgatg
    16981 ctaacggtgg cctgtttgaa acgctgctgg gcgcggaaga cgccattatc tccgacgcac
    17041 tgaaccacgc gtctattatt gatggtgtgc gtctgtgtaa agctaaacgc tatcgctatg
    17101 ccaacaacga tatgcaggag ctggaagcac gtctgaaaga agcgcgtgaa gccggtgcgc
    17161 gtcatgtgct gattgccacc gatggtgtgt tctcgatgga cggcgtgatt gccaacctga
    17221 agggcgtttg cgatctcgca gataaatatg atgccctggt gatggtagac gactcccacg
    17281 cggtcggttt tgtcggtgaa aatggtcgtg gttcccatga atactgcgat gtgatgggcc
    17341 gggtcgatat tatcaccggc acgctcggta aagcgctggg cggggcttct ggtggttata
    17401 ccgcggcgcg caaagaagtg gttgagtggc tgcgccagcg ttctcgtccg tacctgtttt
    17461 ccaactcgct ggcaccggcc attgttgccg catccatcaa agtactggag atggtcgaag
    17521 cgggtagcga gctgcgtgac cgtctgtggg cgaacgcgcg tcagttccgt gagcaaatgt
    17581 cggcggcggg ctttaccctg gcgggagccg atcacgccat tattccggtc atgcttggtg
    17641 atgcggtagt ggcgcagaaa tttgcccgtg agctgcaaaa agagggcatt tacgtcaccg
    17701 gtttcttcta tccggtcgtt ccgaaaggtc aggcgcgtat tcgtacccag atgtctgcgg
    17761 cgcatacccc tgagcaaatt acgcgtgcag tagaagcatt cacgcgtatt ggtaaacaac
    17821 tgggcgttat tgcctgagga tgtgagatga aagcgttatc caaactgaaa gcggaagagg
    17881 gcatctggat gaccgacgtt cctgtaccgg aactcgggca taacgatctg ctgattaaaa
    17941 tccgtaaaac agccatctgc gggactgacg ttcacatcta taactgggat gagtggtcgc
    18001 aaaaaaccat cccggtgccg atggtcgtgg gccatgaata tgtcggtgaa gtggtaggta
    18061 ttggtcagga agtgaagggc ttcaagattg gcgatcgcgt ttctggcgaa ggccatatca
    18121 cctgcggtca ctgtcgcaac tgtcgtggcg gtcgtaccca tctgtgccgc aacacgatcg
    18181 gcgtcggcgt taaccgcccg ggctgcttcg ccgaatatct ggtgattccg gcgttcaacg
    18241 ccttcaaaat tcccgacaat atctccgacg acctggcttc catttttgat cccttcggta
    18301 acgccgtgca taccgcgctg tcgttcgatc tggttggcga ggatgtgctg gtctctggtg
    18361 caggcccgat tggtattatg gcagcggcgg tggcgaaaca cgttggtgca cgcaatgtgg
    18421 tgatcactga tgttaacgaa tatcgtctcg aactggcgcg caaaatgggt atcacccgtg
    18481 cggtcaacgt cgctaaagaa aaccttaatg atgtgatggc agaactgggc atggccgaag
    18541 gctttgatat cggtctggaa atgtccggtg cgccgccagc gtttcgtacc atgctcgaca
    18601 ccatgaacca cggcggtcgc attgcgatgc tgggtattcc accgtccgat atgtctatcg
    18661 actggaccaa agtaatcttt aaaggcttgt tcattaaagg tatttacggt cgtgagatgt
    18721 ttgaaacctg gtacaagatg gcggcgctga ttcagtcagg tctggatctc tcgccgatca
    18781 ttacccatcg tttctctatc gatgatttcc agaagggctt tgacgctatg cgttcgggcc
    18841 agtccgggaa agtaattctg agctgggatt aacacgaaca agggctggta ttccagccct
    18901 tttgcctgag gataatccgt taaatatgta aaatcctgtc agtgtaataa agatatcgta
    18961 attgcgctga tctcttatat agctgctctc attatctctc taccctgaag tgactctctc
    19021 acctgtaaaa ataatatctc acaggcttaa tagtttctta atacaaagcc tgtaaaacgt
    19081 caggataact tcagaggtcg tcggtaattt atgatgaaca gcaccaataa acttagtgtt
    19141 attattccgt tatataatgc gggcgatgat ttccgcactt gtatggaatc tttaattacg
    19201 caaacctgga ctgctctgga aatcattatt attaacgatg gttcaacgga taattctgtt
    19261 gaaatagcaa agcattacgc agaaaactat ccgcacgttc gtttgttgca tcaggcgaat
    19321 gctggcgcat cggtggcgcg taatcgtggg attgaagtgg caacgggcaa atatgtcgct
    19381 tttgtcgatg ctgacgatga agtctatccc accatgtacg aaacgctgat gaccatggcg
    19441 ttagaggacg acctcgacgt ggcgcagtgc aacgctgact ggtgttttcg tgaaacggga
    19501 gaaacctgga aatccatccc cagcgatcgc cttcgctcaa ccggcgtatt aaccggcccg
    19561 gactggctgc ggatggggct ttcttcgcgc cgttggactc acgttgtctg gatgggggtt
    19621 tatcgccgcg atgttattgt taaaaataac attaaattta ttgccggatt acatcatcag
    19681 gatattgtct ggacaacaga atttatgttt aatgcgctgc gtgcgcgata taccgagcaa
    19741 tcgttatata aatattatct gcataatacg tcagtgagtc ggttgcatag acaagggaac
    19801 aaaaacctta attatcaacg tcactatatt aagattactc gtctgctaga gaaattaaat
    19861 cgaaattatg ccgacaaaat tacgatttat ccggaatttc atcagcaaat aacttacgaa
    19921 gcattgcgtg tttgccatgc ggtgcgcaaa gagccggata ttattacccg ccaacggatg
    19981 attgccgaga tatttacttc cggtat
//
LOCUS       DAJVYM010000071        19408 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVYM010000071 DAJVYM010000000
VERSION     DAJVYM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.071"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..245
                     /locus_tag="OQS60_002310"
     CDS             <1..245
                     /locus_tag="OQS60_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCS2388044.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="OQS60_002311"
     CDS             complement(296..1063)
                     /locus_tag="OQS60_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2388045.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="OQS60_002312"
     CDS             1259..2167
                     /locus_tag="OQS60_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS2388046.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="OQS60_002313"
     CDS             2164..3426
                     /locus_tag="OQS60_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS2388047.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="OQS60_002314"
     CDS             3423..4061
                     /locus_tag="OQS60_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS2388048.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="OQS60_002315"
     CDS             4066..4842
                     /locus_tag="OQS60_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS2388049.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="OQS60_002316"
     CDS             4931..6295
                     /locus_tag="OQS60_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS2388050.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OQS60_002317"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OQS60_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS2388051.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OQS60_002318"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OQS60_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS2388052.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OQS60_002319"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OQS60_002319"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS2388053.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OQS60_002320"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OQS60_002320"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS2388054.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OQS60_002321"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OQS60_002321"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS2388055.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OQS60_002322"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OQS60_002322"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS2388056.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OQS60_002323"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OQS60_002323"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS2388057.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OQS60_002324"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OQS60_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS2388058.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="OQS60_002325"
     CDS             complement(12844..13167)
                     /locus_tag="OQS60_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS2388059.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OQS60_002326"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OQS60_002326"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS2388060.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OQS60_002327"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OQS60_002327"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS2388061.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OQS60_002328"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OQS60_002328"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS2388062.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="OQS60_002329"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="OQS60_002329"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS2388063.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OQS60_002330"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OQS60_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS2388064.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OQS60_002331"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OQS60_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="OQS60_002332"
     CDS             complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="OQS60_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS2388065.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4592 a         5231 c         4848 g         4737 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagcg
//
LOCUS       DAJVYM010000072        19244 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVYM010000072 DAJVYM010000000
VERSION     DAJVYM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.072"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            204..280
                     /locus_tag="OQS60_002333"
     tRNA            204..280
                     /locus_tag="OQS60_002333"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Met,seq:cat)
     gene            487..939
                     /gene="rimP"
                     /locus_tag="OQS60_002334"
     CDS             487..939
                     /gene="rimP"
                     /locus_tag="OQS60_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS2388066.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            967..2454
                     /gene="nusA"
                     /locus_tag="OQS60_002335"
     CDS             967..2454
                     /gene="nusA"
                     /locus_tag="OQS60_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS2388067.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            2479..5151
                     /gene="infB"
                     /locus_tag="OQS60_002336"
     CDS             2479..5151
                     /gene="infB"
                     /locus_tag="OQS60_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS2388068.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            5315..5716
                     /gene="rbfA"
                     /locus_tag="OQS60_002337"
     CDS             5315..5716
                     /gene="rbfA"
                     /locus_tag="OQS60_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS2388069.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            5716..6660
                     /gene="truB"
                     /locus_tag="OQS60_002338"
     CDS             5716..6660
                     /gene="truB"
                     /locus_tag="OQS60_002338"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS2388070.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            6809..7078
                     /gene="rpsO"
                     /locus_tag="OQS60_002339"
     CDS             6809..7078
                     /gene="rpsO"
                     /locus_tag="OQS60_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS2388071.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            7325..9460
                     /gene="pnp"
                     /locus_tag="OQS60_002340"
     CDS             7325..9460
                     /gene="pnp"
                     /locus_tag="OQS60_002340"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS2388072.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            9569..10453
                     /gene="nlpI"
                     /locus_tag="OQS60_002341"
     CDS             9569..10453
                     /gene="nlpI"
                     /locus_tag="OQS60_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS2388073.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            10560..10640
                     /gene="yrbN"
                     /locus_tag="OQS60_002342"
     CDS             10560..10640
                     /gene="yrbN"
                     /locus_tag="OQS60_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS2388074.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            10633..12522
                     /gene="deaD"
                     /locus_tag="OQS60_002343"
     CDS             10633..12522
                     /gene="deaD"
                     /locus_tag="OQS60_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS2388075.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            12676..13920
                     /gene="mtr"
                     /locus_tag="OQS60_002344"
     CDS             12676..13920
                     /gene="mtr"
                     /locus_tag="OQS60_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS2388076.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14038..15045)
                     /locus_tag="OQS60_002345"
     CDS             complement(14038..15045)
                     /locus_tag="OQS60_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS2388077.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15251..16129)
                     /locus_tag="OQS60_002346"
     CDS             complement(15251..16129)
                     /locus_tag="OQS60_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS2388078.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            complement(16138..17133)
                     /locus_tag="OQS60_002347"
     CDS             complement(16138..17133)
                     /locus_tag="OQS60_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS2388079.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            17342..17866
                     /locus_tag="OQS60_002348"
     CDS             17342..17866
                     /locus_tag="OQS60_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS2388080.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            17860..18363
                     /locus_tag="OQS60_002349"
     CDS             17860..18363
                     /locus_tag="OQS60_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2388081.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(18350..18652)
                     /gene="yhbQ"
                     /locus_tag="OQS60_002350"
     CDS             complement(18350..18652)
                     /gene="yhbQ"
                     /locus_tag="OQS60_002350"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS2388082.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            18703..19146
                     /locus_tag="OQS60_002351"
     CDS             18703..19146
                     /locus_tag="OQS60_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS2388083.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            complement(19126..>19244)
                     /locus_tag="OQS60_002352"
     CDS             complement(19126..>19244)
                     /locus_tag="OQS60_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HCS2388084.1"
                     /translation="GAEFYDQEVVVDKDQLVTSRTPDDLPAFNREALRLLGA"
BASE COUNT         4621 a         4790 c         5375 g         4458 t
ORIGIN      
        1 ttaaggggga gcaggagaag gcaaaagtat tcaacaaatg aaagtgaact ggatattcat
       61 tcacatgatt agcaataaac gttgacaaaa tgtggcgtgg atcactataa tgcctgcaga
      121 ttttacgtcc cgtctcggta caccaaatcc cagcagtatt tgcatttttt acccaaaacg
      181 agtagaattt gccacgtttc aggcgcgggg tggagcagcc tggtagctcg tcgggctcat
      241 aacccgaagg tcgtcggttc aaatccggcc cccgcaacca ctttccctta gagtcctttt
      301 tcaaatatac tgtgaagact tcggccttcg tagtgggatt tgaaaaaatc cttctggaaa
      361 gtgctccaga ccgcagttgc ggttataggg ttcagttata taaagccccg atttatcggg
      421 gttttttgtt atctgactac agaataactg ggctttaggc ccttttttta tgtcttgggg
      481 gtgggcttgt ccacattaga gcaaaaatta acagagatga ttactgcgcc agttgaggcc
      541 ctgggttttg aactggttgg catcgaattt attcgcggtc gcacatccac actgcgcatc
      601 tatattgata gtgaagatgg catcaatgtt gatgattgtg ctgatgtgag ccaccaggta
      661 agtgctgtgc tggatgttga agatcccatc accgttgctt ataacctgga agtctcctca
      721 ccgggtctcg atcgcccact gttcacggct gaacactacg cccgttttgt cggagaagag
      781 gtgactctgg ttctccgtat ggcggtacaa aaccgtcgta aatggcaggg cgttatcaaa
      841 gcggtagacg gtgaaatgat cacagttacc gtcgaaggta aagatgaagt gttcgcgctg
      901 agtaatatcc agaaggcgaa cctggttccc cacttttaat agtctggatg aggtgaaaag
      961 cccgcgatga acaaagaaat tttggctgta gttgaagccg tatccaatga aaaggcgcta
     1021 cctcgcgaga agattttcga agcattggaa agcgcgctgg cgacagcaac aaagaaaaaa
     1081 tatgaacaag agatcgacgt ccgcgtacag atcgatcgca aaagcggtga ttttgacacc
     1141 ttccgtcgct ggttagttgt tgatgaagtc acccagccga ccaaggaaat cacccttgaa
     1201 gccgcacgtt atgaagatga aagcctgaac ctgggcgatt acgttgaaga tcagattgag
     1261 tctgttacct ttgaccgtat cactacccag acggcaaaac aggttatcgt gcagaaagtg
     1321 cgtgaagccg aacgtgcgat ggtggttgat cagttccgtg aacacgaagg tgaaatcatc
     1381 accggcgtgg tgaaaaaagt aaaccgcgac aacatctctc tggatctggg caacaacgct
     1441 gaagccgtga tcctgcgtga agatatgctg ccgcgtgaaa acttccgccc tggcgaccgc
     1501 gttcgtggcg tgctttattc cgttcgcccg gaagcgcgtg gcgcgcaact gttcgtcact
     1561 cgttccaagc cggaaatgct gatcgaactg ttccgtattg aagtgccaga aatcggcgaa
     1621 gaagtgattg aaattaaagc agcggctcgc gatccgggtt ctcgtgcgaa aatcgcggtg
     1681 aaaaccaacg ataaacgtat cgatccggta ggtgcttgcg taggtatgcg tggcgcgcgt
     1741 gttcaggcgg tgtctactga actgggtggc gagcgtatcg atatcgtcct gtgggatgat
     1801 aacccggcgc agttcgtgat taacgcaatg gcaccggcag acgttgcttc tatcgtggtg
     1861 gatgaagata aacacaccat ggatatcgcc gttgaagccg gtaacctggc gcaggcgatt
     1921 ggccgtaacg gtcagaacgt gcgtctggct tcgcagctga gcggttggga actcaacgtg
     1981 atgaccgttg acgacctgca ggctaagcat caggcggaag cgcacgcagc gatcgacacc
     2041 ttcaccaaat atctcgacat cgacgaagac ttcgcgactg ttctggtaga agaaggcttc
     2101 tcgacgctgg aagaactggc ctatgtgccg atgaaagagc tgttggaaat cgaaggcctt
     2161 gatgagccga ccgttgaagc actgcgcgag cgtgctaaaa atgcactggc caccattgca
     2221 caggcccagg aagaaagcct cggtgataac aaaccggctg acgatctgct gaaccttgaa
     2281 ggggtagatc gtgatttggc attcaaactg gccgcccgtg gcgtttgtac gctggaagat
     2341 ctcgccgaac agggcattga tgatctggct gatatcgaag ggttgaccga cgaaaaggcc
     2401 ggagcactga ttatggctgc ccgtaatatt tgctggttcg gtgacgaagc gtaataaact
     2461 gtagcaggaa ggaacagcat gacagatgta acgattaaaa cgctggccgc agagcgacag
     2521 acctccgtgg aacgcctggt acagcaattt gctgatgcag gtatccggaa gtctgctgac
     2581 gactctgtgt ctgcacaaga gaaacagact ttgattgacc acctgaatca gaaaaattca
     2641 ggcccggaca aattgacgct gcaacgtaaa acacgcagca cccttaacat tcctggtacc
     2701 ggtggaaaaa gcaaatcggt acaaatcgaa gtccgcaaga aacgcacctt tgtgaaacgc
     2761 gatccgcaag aggctgaacg ccttgcagcg gaagagcaag cgcagcgtga agcggaagag
     2821 caagcccgtc gtgaggcaga agaatcggct aaacgcgagg cgcaacaaaa agctgaacgt
     2881 gaggctgcag aacaagctaa gcgtgaagct gctgaacaag cgaaacgtga agctgcggaa
     2941 aaagacaaag tgagcaatca acaagacgat atgactaaaa acgcccaggc tgaaaaagcc
     3001 cgccgtgagc aggaagctgc agagctcaag cgtaaagccg aagaagaagc gcatcgtaaa
     3061 ctcgaagaag aagcacgtcg cgttgctgaa gaagcacgtc gtatggcgga agaaaacaaa
     3121 tggactgata acgcggaacc gactgaagat tccagcgatt atcacgtcac tacttctcaa
     3181 catgctcgcc aggcagaaga cgaaagcgat cgtgaagtcg aaggcggtcg tggccgtggt
     3241 cgtaacgcga aagcagcgcg tccgaagaaa ggcaacaaac acgctgaatc aaaagctgat
     3301 cgtgaagaag cacgcgcagc agtacgtggc ggtaaaggcg gaaaacgtaa aggttcttcc
     3361 ctgcagcaag gcttccagaa gccagctcag gccgttaacc gtgacgttgt gatcggcgaa
     3421 accatcaccg ttggcgaact ggcgaacaag atggcggtta aaggctctca ggtcatcaaa
     3481 gcgatgatga aactgggcgc aatggcaacc atcaaccagg ttatcgatca ggaaaccgca
     3541 cagctggttg ctgaagagat gggccacaaa gttatcctgc gtcgtgaaaa cgagctggaa
     3601 gaggcggtaa tgagcgaccg tgacacgggt gctgcggctg aaccgcgcgc gccggttgtg
     3661 accatcatgg gtcacgttga ccacggtaaa acctctctgc tggactacat tcgttcaacg
     3721 aaagttgcct ctggcgaagc gggcggcatt acccagcaca ttggtgcata ccacgttgaa
     3781 actgaaaacg gcatgatcac cttcctggac accccggggc acgccgcgtt tacttcaatg
     3841 cgtgcacgtg gtgcgcaggc aacggacatc gtagtcctgg ttgttgctgc cgacgacggc
     3901 gtgatgccgc agaccatcga agcaatccag cacgcgaaag cggcgcaggt accggtggtg
     3961 gttgcagtga acaagattga taaaccagaa gccgatccgg atcgtgttaa gaacgaactc
     4021 tcccagtatg gcatcctgcc ggaagagtgg ggcggtgaaa gccagttcgt acacgtatct
     4081 gcgaaagcgg gtaccggcat cgatgaactg ctggacgcta tcctgctgca ggcggaagtt
     4141 ctggagctga aagcggtacg taaaggtatg gcgagcggtg cggttatcga atccttcctc
     4201 gataaaggtc gtggtccggt tgctaccgtt ctggtacgtg aaggtactct gcacaagggc
     4261 gatatcgttc tgtgtggctt cgaatacggt cgtgttcgtg cgatgcgtaa cgaactgggt
     4321 caggaagtgc tggaagcggg tccgtccatt ccggtggaaa tcctcggcct gtccggcgta
     4381 ccggctgcgg gtgatgaagt taccgttgta cgtgacgaga agaaagcgcg tgaagttgca
     4441 ctgtatcgtc agggtaaatt ccgcgaagtt aaactggcgc gtcagcagaa atctaaactc
     4501 gagaacatgt tcgccaacat gaccgaaggc gaagttcacg aagtgaacat cgtcctgaag
     4561 gcagacgtac agggttctgt ggaagcgatc tccgactcct tgctgaaact gtctactgac
     4621 gaagttaaag tgaagatcat cggttctggc gtaggtggta tcaccgaaac cgacgccacc
     4681 ctggctgcgg cgtccaacgc catcctggtt ggcttcaacg tacgtgctga tgcctctgca
     4741 cgtaaagtga ttgaagcgga aagcctggat ctgcgttact actccgtcat ctataacctg
     4801 attgacgaag tgaaagcggc gatgagcggt atgctgtctc cggaactgaa acagcagatt
     4861 atcggtctgg cggaagttcg tgacgtgttc aaatcgccga aatttggtgc tatcgcaggc
     4921 tgtatggtta ccgaaggtgt ggttaaacgt cacaacccga tccgcgttct gcgtgacaac
     4981 gtggttatct acgaaggcga gctggagtcc ctgcgccgct tcaaagatga cgttaacgaa
     5041 gtccgtaacg gtatggaatg tggtatcggc gttaagaact acaacgacgt ccgcactggc
     5101 gatgtgatcg aagtattcga aatcatcgag atccaacgta ccatcgctta aggtttttag
     5161 ggttccatgc gggataaggc gtttacgccg catccggcat ggattgcctg atgtgacgct
     5221 ggcgcgtctt atcaggccaa cgtgaacagc ccttccacct gaaattaatt ttaaaaaggg
     5281 gctaacagcc ccttttttgt caggagaatt tattatggcg aaagaatttg gtcgcccgca
     5341 gcgcgtagcg caggaaatgc aaaaagagat cgctctcatc ctgcagcgtg aaattaaaga
     5401 tcctcgcctg ggcatgatga ccaccgtttc cggtgtcgaa atgtctcgcg acctggcgta
     5461 tgccaaagta tatgtgacgt tcctcaacga caaagatgaa gacgcggtta aagcgggcat
     5521 caaagcgttg caagaagctt ctggtttcat ccgcagcctg ctggggaaag cgatgcgcct
     5581 gcgtatcgtg ccggaactga ccttcttcta cgacaactct ctggttgaag ggatgcgcat
     5641 gtcaaacctg gtgaccagcg tggtcaaaca tgacgaagaa cgtcgtgtta acccggacga
     5701 cagcaaggag gactaatgag tcgtcctcgt cgtcgcggtc gcgacattaa cggcgttttg
     5761 ttgctggata aaccgcaggg tatgtccagc aatgatgcgc tgcaaaaagt gaaacggatt
     5821 tataacgcca accgtgccgg gcataccggt gcgctggacc cgctggcaac aggcatgttg
     5881 ccaatttgcc tcggggaagc gacgaagttt tcccagtatc tgctggactc cgacaaacgc
     5941 tatcgggtca ttgcgcgtct tggacagcgt accgatactt ctgatgccga cggacaaatc
     6001 gttgaagaac gtccggtaac ctttagcgca gagcaactgg cggcggcgct ggatactttc
     6061 cgtggcgata tcgaacagat cccttcgatg tattcagcac tgaaatatca gggtaaaaaa
     6121 ctgtacgaat atgcgcgtca gggcattgaa gtaccgcgtg aagcgcgccc gattaccgtt
     6181 tatgaattgc tgtttattcg ccacgaaggc aatgagctgg agctggaaat tcactgctca
     6241 aaaggcactt atatccgcac catcattgat gacctgggtg aaaaactcgg ttgtggcgcg
     6301 catgttattt acctgcgtcg tctggcggta agtaaatatc cggttgaacg gatggtgacc
     6361 ctggaacatc tgcgtgaact ggtcgaacaa gccgaacagc aggatattcc ggctgcggag
     6421 ttacttgatc cattactgat gccaatggac agtccagctt cggactaccc ggtggtgaat
     6481 cttccgttaa cgtcttctgt ttacttcaaa aatggtaacc cagttcgtac atccggtgcg
     6541 ccgctggaag gactggttcg cgttacagaa ggtgagaacg gcaagtttat cggtatgggc
     6601 gaaattgacg atgaaggccg cgtcgcgcct cgtcgcctgg tggttgaata cccggcgtaa
     6661 tgttaaccgt cttgcgataa caggtcgcta cgagtagaat actgccgctt aacgtcgcgt
     6721 aaattgttta acactttgcg taacgtacac tgggatcgct gaattagaga tcggcgtcct
     6781 ttcattctat atactttgga gttttaaaat gtctctaagt actgaagcaa cagctaaaat
     6841 cgtttctgag tttggtcgtg acgcaaacga caccggttct accgaagttc aggtagcact
     6901 gctgactgca cagatcaacc acctgcaggg ccactttgca gagcacaaaa aagatcacca
     6961 cagccgtcgt ggtctgctgc gcatggtttc tcagcgtcgt aaactgctcg actacctgaa
     7021 acgtaaagac gtagcacgtt acacccagct catcgagcgc ctgggtctgc gtcgctaatt
     7081 cttgcgagtt tcagaaaagg gggcctgagt ggcccctttt ttcaagctga cggcagcaat
     7141 tcactggaaa ctaatgtatt gttgctatga atgatcttcc gttgcagagg ttcgcgcggc
     7201 taatgagagg ctttacccac atagagctgg gttagggttg tcattagtcg cgaggatgcg
     7261 cagaagatcg ggtattaaca ccagtgccgt aaggtactgt ctaagaaaga gaaaggatat
     7321 tacattgctt aatccgatcg ttcgtaaatt ccagtacggc caacacaccg tgactctgga
     7381 aaccggcatg atggctcgtc aggctactgc cgctgttatg gttagcatgg atgacaccgc
     7441 ggtattcgtt accgttgttg gacagaaaaa agccaaacca ggtcaggact tcttcccact
     7501 gaccgttaac tatcaggagc gtacctacgc tgctggtcgt atcccgggta gcttcttccg
     7561 tcgtgaaggt cgtccaagcg aaggcgaaac cctgatcgcg cgtctgattg accgcccgat
     7621 tcgcccgctg ttcccggaag gcttcgtcaa cgaagttcag gttatcgcca ccgtggtttc
     7681 tgttaacccg caggttaacc cggatatcgt cgcgatgatt ggtgcttccg cagcactgtc
     7741 tctgtctggt attccgttca atggcccgat tggtgctgcc cgcgtaggtt acatcaatga
     7801 ccagtacgta ctgaacccga ctcaggacga gctgaaagag agcaaactgg atctggttgt
     7861 tgccggtact gaagccgctg tactgatggt tgaatctgaa gctgaactgc tgagcgaaga
     7921 tcagatgctg ggcgcagtag tgttcggtca tgaacaacag caggttgtta ttcagaacat
     7981 caatgaacta gtgaaagaag ccggtaaacc gcgttgggac tggcagccgg agccggtaaa
     8041 cgaagcgctg aacgcgcgcg ttgctgcact ggctgaagct cgtctgagtg atgcttaccg
     8101 catcaccgac aaacaagagc gttatgcgca ggttgatgtc atcaaatctg aaaccatcgc
     8161 gacgctgctt gctgaagacg aaaccctgga cgaaaacgaa ctgggtgaaa ttctgcacgc
     8221 tatcgagaaa aacgttgttc gtagccgcgt actggcaggc gaaccgcgta tcgacggtcg
     8281 tgaaaaagat atgatccgtg gtctggatgt gcgtactggc gtgctgccgc gtactcacgg
     8341 ttctgcgctg ttcacccgcg gtgaaacgca ggcactggtt actgcaacgc tgggtaccgc
     8401 tcgtgacgcg caggttcttg atgaactgat gggcgaacgt accgatacct tcctgttcca
     8461 ctacaacttc cctccgtact ccgtaggcga aaccggcatg gtcggttctc cgaagcgtcg
     8521 tgaaattggt cacggtcgtc tggcgaagcg cggcgtgctg gcagtcatgc cggatatgga
     8581 caaattcccg tacaccgtac gtgtagtgtc tgaaatcacc gaatccaacg gttcttcttc
     8641 tatggcttcc gtgtgcggcg cgtctctggc gctgatggac gcaggtgtgc caatcaaagc
     8701 tgccgttgcg ggtatcgcaa tgggtctggt gaaagaaggc gacaactacg ttgtactgtc
     8761 tgacattttg ggcgacgaag atcacctggg cgatatggac ttcaaagttg caggttcccg
     8821 cgacggtatc tctgcactgc agatggatat caaaattgaa ggtatcacca aagagatcat
     8881 gcaggttgcg ctgaaccagg ctaaaggtgc gcgtctgcat atcctgggcg taatggaaca
     8941 ggcgatcaac gcgccgcgtg gcgatatctc tgagttcgca ccgcgtatcc ataccatcaa
     9001 gatcaacccg gataagatca aagacgttat cggtaaaggc ggctctgtta tccgtgccct
     9061 gaccgaagaa actggcacta ccatcgaaat cgaagatgac ggtactgtga agatcgcagc
     9121 gaccgacggc gagaaagcga aacatgctat tcgtcgtatc gaagagatca ctgcagaaat
     9181 cgaagtgggc cgcgtctaca ctggtaaagt gacccgtatc gttgactttg gcgcatttgt
     9241 tgccatcggc ggcggtaaag aaggtctggt ccacatctct caaatcgctg acaaacgcgt
     9301 tgagaaagtg accgattacc tgcagatggg tcaggaagta ccggtgaaag ttctagaagt
     9361 tgatcgccag ggccgtatcc gtctgagcat taaagaagcg actgagcagt ctcaacctgc
     9421 tgcagcaccg gaagctccgg ctgctgaaca gggcgagtaa ggttgccatt tgccctccgc
     9481 tgcggcgggg ggcttttaac cgggcaggac gccttgttag caaccgggaa caggacgttc
     9541 attcaaccgt ggtcttcggg agtgggaaat gaagcctttt ttgcgctggt gtttcgttgc
     9601 gacagcactt acgcttgcag gatgcagtaa tacttcctgg cgtaaaagtg aagtcctcgc
     9661 ggtaccattg caaccgactt tacagcagga agtgattctg gcacgtatgg aacaaatcct
     9721 tgccagtcgg gctttaaccg atgacgaacg cgcacagctt ttatatgagc gcggagtgtt
     9781 gtatgatagt ctcggtctga gggcattagc gcgtaacgat ttttcgcaag cgctggcaat
     9841 ccgaccggat atgcctgaag tattcaatta cttaggcata tatttaacgc aggcaggcaa
     9901 ttttgatgct gcctatgaag cgtttgattc tgtacttgag cttgatccaa cttacaacta
     9961 cgcgcacttg aatcgcggga tcgcattata ttacggcggt cgtgacaagt tagcgcaaga
    10021 tgatctgctg gcgttttatc aagacgatcc caatgatcct ttccgtagtc tgtggcttta
    10081 tctcgccgag cagaagctcg atgagaagca ggctaaagaa gtgttgaaac agcacttcga
    10141 aaaatcggat aaggaacagt ggggatggaa cattgtcgag ttctacctgg gcaacattag
    10201 cgaacaaacg ttaatggaaa ggctcaaggc ggacgcaacg gataacacct cgctcgctga
    10261 gcatctcagt gaaaccaact tctatttagg taagtactac ctaagtctgg gggatttgga
    10321 cagcgccacg gcactgttca aactggcggt tgccaacaac gttcataact ttgttgagca
    10381 ccgatacgca ttgttggaat tatcgctcct gggccaggac caagatgacc tggcagaatc
    10441 ggaccagcaa tagctgacgt acacatcagc ccgtaatctt ttttgattgc catcacctta
    10501 acgggtgagg gcgttgttgt tcgttaatac acctactttg agccggttca cacttttcaa
    10561 tgaaaattgc tgatcaattt catgatgagt tatgtagact ggccgccatt aattttgagg
    10621 cacacgtact acatggctga attcgaaacc acttttgcag atctgggcct gaaggctcct
    10681 atccttgaag cccttaacga tctgggttac gaaaaaccat ctccaattca ggcagagtgt
    10741 attccacatc tgctgaatgg ccgcgacgtt ctgggtatgg cccagacggg gagcggaaaa
    10801 actgcagcat tctctttacc tctgttgcag aatcttgatc ctgagctgaa agcaccacag
    10861 attctggtgc tggcaccgac ccgcgaactg gcggtacagg ttgctgaagc aatgacggat
    10921 ttctctaaac acatgcgcgg cgtaaatgtg gttgctctgt acggcggcca gcgttatgac
    10981 gtgcaattac gcgccctgcg tcaggggccg cagatcgttg tcggtactcc gggccgtctg
    11041 ctggaccacc tgaaacgtgg cactctggac ctctctaaac tgagcggtct ggttctggat
    11101 gaagctgacg aaatgctgcg catgggcttc atcgaagacg ttgaaaccat tatggcgcag
    11161 atcccggaag gtcatcagac cgctctgttc tctgcaacca tgccggaagc gattcgtcgc
    11221 attacccgcc gctttatgaa agagccgcag gaagtgcgca ttcagtccag cgtgactacc
    11281 cgtcctgaca tcagccagag ctactggact gtctggggta tgcgcaaaaa cgaagcactg
    11341 gtacgtttcc tggaagcgga agattttgat gcggcgatta tcttcgttcg taccaaaaac
    11401 gcgactctgg aagtggctga agctcttgag cgtaacggct acaacagcgc cgcgctgaac
    11461 ggtgacatga accaggcgct gcgtgaacag acactggaac gcctgaaaga tggtcgtctg
    11521 gacatcctga ttgcgaccga cgttgcagcc cgtggcctgg acgttgagcg tatcagcctg
    11581 gtagttaact acgatatccc gatggattct gagtcttacg ttcaccgtat cggtcgtacc
    11641 ggtcgtgcgg gtcgtgctgg ccgcgcgctg ctgttcgttg agaaccgcga gcgtcgtctg
    11701 ctgcgcaaca ttgaacgtac tatgaagctg actattccgg aagtagaact gccgaacgca
    11761 gaactgctgg gcaaacgccg tctggaaaaa ttcgccgcta aagtacagca gcagctggaa
    11821 agcagcgatc tggatcaata ccgcgcactg ctgagcaaaa ttcagccgac tgcagaaggt
    11881 gaagagctgg atctcgaaac tctggctgcg gcactgctga aaatggcaca gggtgaacgt
    11941 actctgatcg taccgccaga tgcgccgatg cgtccgaaac gtgaattccg tgaccgtgat
    12001 gaccgtggtc cgcgcgatcg taacgaccgt ggcccgcgtg gtgaccgtga agatcgtccg
    12061 cgtcgtgaac gtcgtgatgt tggcgatatg cagctgtacc gcattgaagt gggccgcgat
    12121 gatggtgttg aagttcgtca tatcgttggt gcgattgcta acgaaggcga catcagtagc
    12181 cgttacattg gtaacatcaa gctgtttgct tctcactcca ccatcgaact gccgaaaggt
    12241 atgccgggtg aagtgctgca acactttacg cgcactcgca ttctcaacaa gccgatgaac
    12301 atgcagttac tgggcgatgc acagccgcat actggcggtg agcgtcgtgg cggtggtcgt
    12361 ggtttcggtg gcgaacgtcg tgaaggcggt cgtaacttca gcggtgaacg ccgtgaaggt
    12421 ggccgtggtg atggtcgtcg ttttagcggc gaacgtcgtg aaggccgcgc tccgcgtcgt
    12481 gatgactcta ccggtcgtcg tcgtttcggt ggtgatgcgt aatcatcgct gaacagcgaa
    12541 cacaatctgt aaaataatat atacagcccc gatttttacc atcggggctt tttttctgtc
    12601 ttttgtactc gtgtactggt acagtgcaat gcataacaac gcagtcgcac tatttttcac
    12661 tggagagaag ctctcatggc aacactaacc accacccaaa cgtcaccgtc gctgcttggc
    12721 ggcgtggtga ttatcggcgg caccattatt ggcgcaggga tgttttctct gccagtggtc
    12781 atgtccgggg cgtggttttt ctggtcaatg gcggcgctga tctttacctg gttctgtatg
    12841 ctgcattccg gcttgatgat tctggaagct aacctgaatt accgaatcgg ttcgagtttt
    12901 gacaccatca ccaaagattt gctgggcaaa ggctggaacg tggtcaacgg tatttccatt
    12961 gcctttgtgc tctatatcct gacctatgcc tatatttctg ccagtggttc aattctgcat
    13021 cacaccttcg cagagatgtc gctaaacgtc ccggcacggg cggcgggttt tggttttgca
    13081 ctgctggtag cgtttgtggt gtggttgagt actaaggccg tcagccgcat gacggcgatt
    13141 gtactggggg cgaaagtcat taccttcttc ctcaccttcg gtagcctact ggggcatgtg
    13201 cagcctgcga cattgttcaa cgtcgccgaa agcaatgcat cttatgcgcc gtatttgctg
    13261 atgaccctgc cgttctgtct ggcatcgttt ggttatcacg gtaacgtgcc gagcctgatg
    13321 aagtattacg gcaaagatcc gaaaaccatc gtgaaatgcc tggtgtacgg tacgctgatg
    13381 gcactggcgc tgtataccat ctggttgctg gcgacgatgg gtaacatccc gcgtccggag
    13441 tttatcggta ttgcagagaa gggcggtaat attgatgtgc tggtacaggc attaagcggc
    13501 gtgctgaaca gccgtagcct ggaccttctg ctggtcgtgt tctcaaactt tgcggtagcg
    13561 agttcgttcc ttggcgtaac gctgggtttg tttgactatc tggcagatct gtttggtttc
    13621 gatgactcag ccctggggcg cttgaaaacg gcattgctga cctttgcccc gcctgttgtg
    13681 ggtggtctac tgttcccgaa cggattcctg tacgccattg gttatgctgg tttagcagct
    13741 accatctggg cggcaattgt tccggcgctg ttagcccgcg catcgcgtaa acgctttggc
    13801 agcccgaaat tccgtgtctg gggcggcaag ccgatgatta cgctgattct ggtgtttggc
    13861 gttggcaacg cactggtcca tatcttatcg agctttaatt tgctgccggt gtatcagtaa
    13921 tcagcggtgc cttatccgac atttctgctg cctacacaat gcctgatgcg cttcgcttat
    13981 caggtctacg taggacagcg ttgccagctc ggataaggct tcccgcgtta agacacacta
    14041 tcccaacaac tcttccttca catccatcgc cagttcaaac gaatgcaggc gcgcctggtg
    14101 gtcgaaaatc tgcccgttaa ccataatttc atcggcgtcg gtttcgcgca ggatcgactg
    14161 caagccgtga cgcactttcg ctttatcacc caccaacgac atactcagcg cctgctgcac
    14221 gccatactgc tcagacggtg accagaactg atccatattt tgaatcggcg gcggcagttg
    14281 tccggtttcg ccacggcgca gcttcacaaa ggcttgctgc attgaggtaa atagaaattc
    14341 agcgtcgcgg ttgctgtcgg cggcgataat attgatgcac accatcgcgt atggtttttc
    14401 cagtcgtgcc gacggtttga agttgctgcg ataaagatgc agcgcctgga acaacatatc
    14461 cggcgcgaag tgtgaggcaa acgcgaacgg cagaccaagc tgcgccgcca gttgcgcgct
    14521 gtaaaggctg gagcctaaca accacacggg gattttctcg ccatagcctg gtaccgggcg
    14581 cacatgcgga ttgggatcgc gggcgtcaaa ccagtccacc agctccgcga catcgcgggg
    14641 gaaattatca atatcgccgc tcatatggcg acgcagcgcc atcattgtcc gttggtcact
    14701 acctggcgca cgacccagcc ccaaatcgat tcgccccgga tagagtgtat tgagcgtgcc
    14761 gaactgttcg gcaatgacca acggtgagtg gttaggcaac atcacgccgc cagaccccag
    14821 atgcagcgtg gtggtattcg ccgccagata gccaatcaat accgacgtgg cagcactggc
    14881 aatgccagtc atattgtggt gttctgccag ccagtagcga tgatagccgc gcttttcagc
    14941 cagacgggcg agatcgagag agtgggagaa tgcttctcgc gctgaagaac cttcgggaat
    15001 gggggccaga tcgagtagcg aaaacgcaat ggttttatca gtcataacag ctcactttgt
    15061 taccgattaa tctttaatac tgcattaaag agtagttaaa gttgttaaca aagtgagctg
    15121 tgctctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca
    15181 taagtaagtg actggggtga acgaatgcag atgcagcaca tgcaacttga agtatgacga
    15241 gcatatttac ttaggcttgc agctccagcc cagccagccg ccgccagtag ccgttacagt
    15301 cgctgtttgc cgtcaacggc agtggcgcag cgccattttc attagcgcgg aaggcgtcga
    15361 gcatcgcgaa agtgtcagta ccctgcggtg acaggcggac cacatcaacc aatccctgca
    15421 tggatgtcag ctcgttaccg aggttgtaaa cgtagccgct catggtctga atgccattga
    15481 gtacaaacac ttgttggttt tcctgcgaca gcacgttgcg cccgttcgga tacttaatgc
    15541 agcaggtttc acactcatct ttcgggcggt cttccgaacg cgcggtaaag cagcgggcgg
    15601 agtaggccag cggtagatga ccgtagctca ggacttccac ttcaaactgg ttgcgaatcc
    15661 ccaactcatc gcactgatta agcagattca ccagccagtc gcgggaaagc tccaccggca
    15721 tacaccagcg catcatgccc tgtttgagca atattttcag cgtcaccgcg ttgtagcagt
    15781 tcagcgcgtg cccggcgacg aacggcagtt tgcgttcagc gcacatattc accacgccga
    15841 gatcgctggc ttcaatcaga aactcaccgt tctcaacata gcgtttcagt tcgcccagtt
    15901 cagaggatgc ctgcaccagc gccagcgtgg agagcactat ctgcttcccg cttccggcga
    15961 gcgattttgc catctccagc cagtcgccaa ctttggttgc ccggcgcttg ctgcataccg
    16021 cttcaccaag ataaatcacg tcggcgctgc tggtggcggc ctgctgataa aattcttcca
    16081 gcgcctcttt tggccagtac cacagcactg gccctaagga atatttcatt gctcttctca
    16141 ctgccattta cggtgatacg cgccaagggt ggtctgcgtg ccttcggaca tcgacccgag
    16201 cgtctccatc cacgcgcttt gcggtacgaa gttttgcgga tcggccttac aacggtcgat
    16261 agcctgacgc cagactttcg ccacctggct gacatacgcc gggctacgct ggcggccttc
    16321 aattttcacc gaagcaatat tcgccgccat taactccggc agcagttcca gggtattgag
    16381 actggttggt tcttccagcg cgtgatagcg ctcgccgtcc accagataac gccctttaca
    16441 cagcgtcgga taacctgcgt tttcgccatc ctgataacgg tcgatcagca cttcgttcag
    16501 gcgggattcc agcccctgcg gcgtttgttg ccagcgcacg aaacgggccg gagaacacgc
    16561 gcccacggtg ttgggcgact cacccgtcag atacgacgac agatagcaac gaccttccga
    16621 cataatgcac aggctgccaa aagcaaagac ttccagcggg acaggtgtga cccgtgccag
    16681 ctgtttcacc tgatgaatcg acaacacgcg cggcagcacc acgcgggcaa catcaaaatg
    16741 gcgatgataa aagttaatcg cctcttcatt ggtcgccgaa gcctgcactg acacatgacg
    16801 ctcaatatgc gggtaacgct cggcggcgta ctccagcatg gcgaggtcgg cgaggatcag
    16861 cgcgtcggca cccagctgcg ccgccatatc cacggcgcgc tgccaacggg cgtaaccgtc
    16921 cggatgcgca aaagtgttaa tcgcgatgtg aagtttgcgg cgatgttgat ggacaaaact
    16981 caccgcttcc tgcaattttt tctcggtaaa gttaaggccg gcgaagtgac gggcattagt
    17041 atcatctttt agcccgatat aaacagcatc tgcgccgttt tcgatggccg ccttaagcgc
    17101 cgggagattt ccggcagggc agagcagctc cataattttt cctgaaggtt gcgctttcaa
    17161 aacgcaaaaa tgttaacgaa ctgggatttt agttaacctt gctgcgacaa tttttgattt
    17221 aaggcagtta aagttgtatt ggtggtagca gcaatttcat atggaattgt tgatttatac
    17281 cgctatgtta tttttctgat atggcaaaat aggatgattg ttgaaacagg gagtaaaact
    17341 cgtgttggat aaactgcgtt cccgtattgt gcatttgggg ccatctctgt tgagtgtacc
    17401 ggtaaaactg acgccatttg cgctaaaacg ccaggttctt gagcaggtct taagctggca
    17461 attccgtcag gcgctggatg atggtgagct ggagtttctt gaaggccgct ggttaagtat
    17521 tcatgtgcgt gatattgacc tgcaatggtt tacctcggtg gtgaatggca aactggtggt
    17581 cagccagaac gcgcaagctg atgtgagttt tagtgccgac gccagcgatc tgctgatgat
    17641 tgcggcgcgt aaacaagatc cggatacgct cttcttccag cgtcggctgg tgattgaagg
    17701 cgatacggag ctggggctgt atgtgaaaaa cctgatggac gccattgaac tggagcaaat
    17761 gccgaaagcc ttacgcatga tgctgctgca actggcggat tttgttgagg cgggaatgaa
    17821 aaccgcgcct gaaaccaaac agacatcggt aggtgaacca tgctaattcg agtagaaatt
    17881 cccattgatg cgccgggtat tgatgccctg ctgcgtcgct cattcgaaag tgatgcggaa
    17941 gcgaagctgg ttcacgatct gcgtgaagat ggctttctga cgctggggct ggtggcgaca
    18001 gatgacgaag gtcaggtcat tggctatgtg gcatttagtc cggttgatgt gcagggcgaa
    18061 gacctgcaat gggtcggcat ggcaccgttg gcggttgatg aaaaataccg tggacagggg
    18121 ctggcacgcc aactggtcta tgaaggactc gattcgctta atgagttcgg ctatgccgca
    18181 gtggtgacgc tgggcgatcc ggcgctgtac agtcgtttcg gctttgaact ggcggcgcat
    18241 cacgatctgc gttgccgctg gccgggcact gaaagcgcct tccaggtaca tcgtttagcg
    18301 gatgacgcgc tgaatggcgt aaccggtctg gttgagtatc acgagcattt caatcgcttt
    18361 taatctccgg ggtttgcaga ctgcttaaca gctctgcaaa ccctgcgcct tccgctacca
    18421 gacgctcttt ttgccgtttg gtcagttgct taacgcgata ttccgcccgt aacgccagcg
    18481 aacgatcgcc cactggcgca gaaaacgcca gcgtcagttc tcctttcccg cgcagtgctt
    18541 tcgccccttt gccgctttgg tgctgctgat agcggcgttc gacatccgtg gtgatcccgg
    18601 tataaagctt attgtcggcg gtacggatca agtaaagaaa ccagggtgtc atcgtctgcg
    18661 tcgcatgtta aggtcaggcc cacagtataa aagagagcag caatggaaac actcatcacc
    18721 atcagccgtt ggttggcaaa acaacatgtt gtcacctggt gtgtgcagca ggaaggggaa
    18781 ctgtggtgcg ctaatgcttt ttatctgttt gatgcgcaga aagtcgcctt ctacattttg
    18841 acggaagaaa aaacgcgcca cgcgcagatg agtgggccgc aggctgcggt tgccggaacg
    18901 gtaaacggtc agccgaaaac ggtagcgtta attcgcggcg tgcagtttaa aggtgagatc
    18961 cgcaggctgg aaggtgagga aagcgacctc gcgcgccagg cgtacaatcg tcgctttccg
    19021 gttgccagaa tgctgtcggc cccggtgtgg gaaatccggc tcgatgaaat caaattcacc
    19081 gacaacacgc tgggctttgg taaaaaaatg atttggctac gcgactcagg caccgagcag
    19141 gcgtaacgcc tcgcggttaa acgctggcag atcgtccggt gtccggctgg taaccagctg
    19201 atctttatca accacgactt cctgatcgta aaattccgcg cccg
//
LOCUS       DAJVYM010000073        19094 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVYM010000073 DAJVYM010000000
VERSION     DAJVYM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.073"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="OQS60_002353"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="OQS60_002353"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS2388085.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="OQS60_002354"
     CDS             834..1262
                     /locus_tag="OQS60_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2388086.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="OQS60_002355"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="OQS60_002355"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS2388087.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALMHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="OQS60_002356"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="OQS60_002356"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS2388088.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OQS60_002357"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OQS60_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS2388089.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OQS60_002358"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OQS60_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS2388090.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OQS60_002359"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OQS60_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS2388091.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OQS60_002360"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OQS60_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS2388092.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OQS60_002361"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OQS60_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS2388093.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="OQS60_002362"
     CDS             complement(8281..9465)
                     /locus_tag="OQS60_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2388094.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OQS60_002363"
     CDS             complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OQS60_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS2388095.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OQS60_002364"
     CDS             complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OQS60_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS2388096.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OQS60_002365"
     CDS             complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OQS60_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS2388097.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OQS60_002366"
     CDS             complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OQS60_002366"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS2388098.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OQS60_002367"
     CDS             complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OQS60_002367"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS2388099.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OQS60_002368"
                     /pseudo
     CDS             complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OQS60_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OQS60_002369"
     CDS             complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OQS60_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS2388100.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17407..17736)
                     /locus_tag="OQS60_002370"
     CDS             complement(17407..17736)
                     /locus_tag="OQS60_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS2388101.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17888..18232
                     /locus_tag="OQS60_002371"
     CDS             17888..18232
                     /locus_tag="OQS60_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS2388102.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OQS60_002372"
     CDS             complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OQS60_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS2388103.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4794 a         4813 c         4932 g         4555 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccaggaagga
       61 taacgttgcg ccagcaacgg cccgaagggc gaacgtcagt gagtcatcct cccggatgca
      121 ccatcttctc caaaaaattt ccttcaaaaa tatctcattt ctaaataact cctttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatatt caggcgatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagcgt cgaaccgctt
      541 cctcttgttg atgtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgcca ttattttgac
      661 gccgtcgtcg ctgccgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc cgcaggtatg gacgctgtgg acgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagtcat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctatcggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag cgaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg atgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gctgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgacgcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacacctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa atttttaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga acgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgccgccacc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag gatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttt ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagtgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa tgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc acggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgcttttgcg
     5941 agggcgattt tctgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagcacgtcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatctgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcatg
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgccg cgccaaccag taacccaggc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcttggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gctgcttcaa cggcgggata accttgcagc aagccgctgc cagagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgct gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaacgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taactagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttaccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatga caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaagtt ggcaaaggtc aggcagccca gcaatgcgcg
     8821 ggtgcggagg atcttatcgc tgataaacat gctgaaaacg gaacctaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcta gcgccatcag
     8941 tgccattaac accgaggcaa cccaaaagac ggtgcgccag ccgccgaggt ttgccagtaa
     9001 cccggcaacg gttcgtgcca gcaagatccc caacagcaga ccgctcataa tggtgccaac
     9061 cactttgccg cgtttatccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgactgag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaactg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgggcgt aatagttact ggctaccgcc tgaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatggggg cgaaagatag
     9541 cttgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg gaatgagtgt
     9661 attttgtagg catgataaga cgcgccagcg tcgcatcagg cattgtgcac agaatggcgg
     9721 atgaggcaaa aacgacttat ccgcccgaat aaaaattact tctgcgctgc cagcgcctca
     9781 ttcacccagc catcgaactg ctgctggtgg gctttgatcc aaccatcaac atggccctga
     9841 atatcgcctt ctgaggcttt gccgtcatgc ataatggcgt tctgggcgtt aatatctgcc
     9901 actggcaact gcataatggc aaaaagtttc gctgctgccg ggtttttctc ggcccaggct
     9961 ttgttggcaa cgatatgcat ggtgctgacc gggaagccat aattcgcacc attcggcagt
    10021 ttggtatcgg cgtttttatc gcccggcagt gcggagaacg gcacctgcaa ccagaccaca
    10081 tctttccctg gcttcagctc attactcacc cagtacggcg tccaggtgta ataaaacacc
    10141 ggtttgccct ctttgtagcg actgatggtg tcggccatca tcgctgcgta gttcccctga
    10201 ttatgcgtca cggtgttggt cagttcatac gcggcaagct ggtggttgat cgcaccttcg
    10261 cagccccagc ctgggttaca gccggttaaa tccgctttac cgtcgccgtt ggtatcgaac
    10321 agtttggcga tcttcggatc tttcagttgt gcgatgttgg tgattttgta ctggtcggcg
    10381 gttttcttat cgatcaggta accctgtgcc gcgccgttaa cgaatatccc ttcacaataa
    10441 aatttcttat cgccaccggc agcttcgtac atgttgtcat gcagcggcgt ccagttcacg
    10501 gcggtgaagg ttgcatcgcc ggaagcaagt gaggtgtagc caacgttgta atccacttcg
    10561 ctgggtttat tgacggtata acctaatttc tccagtgcac gactgaccag cagcgtctgg
    10621 aaggtttctt cagtgatggt gctctgaact ggattaacag taatgccttt gcccggcaga
    10681 tcggctgcaa aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa aagtacgcta
    10741 tgtcgcattg ttattccttt ttttggcagg gtgataatgc ccggcgttac cgggcaagtg
    10801 cagagttact taatgaatgg gcgggtcagc agaccgacag ggccagtggt gtaccagcga
    10861 cggttgccgc gactgcgtga gtcgcgccca acggcctgcg tcaggcggtc gaggataatg
    10921 gcgaggatca caatcccgac gccgccaacg gtggcaaggc ccatatccag acgaccgata
    10981 ccgcgaagta ccatctgacc cagcccgccg acggcaatca tcgaggcgat gaccaccata
    11041 gaaagagcca gcatcagcgt ctggttaacg cccgccataa tggtcggcat cgccagcggt
    11101 aactgaactt tgaacagcat ctggcgcggg ctggcaccga atgagcgcga ggcttcaatc
    11161 agatccgccg gaacctggtt aatccccaga atcgtcagac ggataatcgg cggcagagca
    11221 aagatgatcg tcaccactac gcccggcacg ttaccgatac caaacagcat gacgattggt
    11281 accagataaa caaacgctgg cgtggtctgc atggcatcaa gcagtggacg aataattttc
    11341 gccgctcgcg gacttctcgc cagccatatc cccaacggca aaccgatgac gatacagaac
    11401 agcagggcgg ttaacaccag cgccagagtc accattgcct gcgaccaggc accgattgcg
    11461 ccgatggcaa tcagcgaaac cagcgtcgcc acgcccattc cgaccccgga aatctgccag
    11521 gcgatgagag cgaaaacgat aatcgccact ggcgctggca tacccagcag caattgctgg
    11581 aaactgttga ggatataatc aaccggaacg cgcacgccct ggaagacggg acggaaatgg
    11641 gtaacaaccc agtcgatccc ttcagtgacc caactgtcga gcgggatcag cgttttatgg
    11701 aacggatcga gaatattaaa atgctcgacg tttggcgcag gcgtactggt cagccagtca
    11761 gcaccaccgc cgtcagtcgg tgcagtcgtc ggtgtacccc aggcgtctgc ggattgcgcg
    11821 gcactgtccg ccgctggcgt ggtatcccac ggattatttt gatcagccat tatttacccc
    11881 ctcacgatct aaagcgcgca gcagcattcc tttcgaaatg atgccgacat actgttggtc
    11941 ctcgtcgacc acgggcaccg cacagggtgc ctgtccgaca tgagagagca actcgctaag
    12001 aggcgtttgt gcatcgactg ctaacggcgc atcaatcagc gccgcatcaa gaccttgctg
    12061 ctgcgttaac gcggttttaa gcgaatcgat ggagactgcg ccgacaaact tattaccgcg
    12121 ttcgataacg tagccatatt cgcgatcttc atcctgcaat aatttcagtg ccgaacgtgg
    12181 gccgaagcca ggggttttac gaattaagcc attcggtgtc cggcgggcaa tatctttcgc
    12241 actgaatacc tgactaatat caacgccacg gaagaaggta cggacataat cattcgccgg
    12301 attattgaga atttcatccg gtgtgccgac ctgtaccact tcaccatttt gcataatggc
    12361 aattcggtcg ccaatacgca tggcttcatc gagatcgtgg gaaataaaga caatggtgcg
    12421 ctgatgtttc gcctgtaatt ttaccagctc atcctgcatc tcggtgcgaa ttaatggatc
    12481 gagcgccgag aaggcttcgt ccattaataa aatatccgga ttaatcgcta acgcgcgggc
    12541 taatcccaca cgttgacgca tcccgccaga gagttcatcc gggtagctgt gggcataatt
    12601 ttccagcccg acctgacgca gtgcatcaag ggctttttcc cggcgttctt cggcattaat
    12661 tccggccaat tccataccga acgcagtatt gtccagcacg gtcatatgcg gcattaaggc
    12721 aaaggactgg aagaccatcg caatcttttt tctgcgcacc tcacggagtt tggcatcaga
    12781 tattttggca atatccacac catcaatcag cacttgcccg cgggtgggtt caatcaggcg
    12841 attgagaagg cgtaccattg tggatttacc cgagccggat aatcccatga tgacaaatat
    12901 ctcgccttct tcaatggcca gactggcgtc ttttacgcca agcgatagcc cagttttttc
    12961 cagaatttgt tcttttgaaa gtccttgttc gatatatttg aatgctcgct gtggatgctc
    13021 accaaatatt ttataaaggt ttttaatttc taatttaatt gccatgcaat agaaagattc
    13081 ctgttatttg tttatgtcga tatattaccc aatggaaata gttacttttt tctaccctaa
    13141 catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt gatgcgagca
    13201 aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag ttgatatttt
    13261 ttgtcaaaga tagcgaaaaa acgcctgaat attgtggttt caggcgaata atgcaacaga
    13321 gtatcagtgt agatcaccac aaaatatttg cgtgataaaa agctatttgg cgggaattat
    13381 ttccctgtcg cgggtagtga tatttttgaa aataacaccc tcagaaattc cagtcttcat
    13441 cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa aaaaaatcgt
    13501 gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact tccgccattt
    13561 ctgcgggaaa taaaggttcg tagcccagat tcatcaaagc cttattggcg ttgtaacaga
    13621 gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg gtgtattgca
    13681 gctcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac tcttcacgtt
    13741 gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag taaccgtgga
    13801 ctgcttcatc acggataatc agacggatca ggtctgcggt attggtcagc tttccgcggc
    13861 tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca agaaacacac
    13921 tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata atctgagctt
    13981 ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca tctttggtct
    14041 ggcacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc ataaagctga
    14101 tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc gaaggcgcgc
    14161 cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc atcgtcagtt
    14221 gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc ggcacctttt
    14281 ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta tcgtcagata
    14341 tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct taaagtgcac
    14401 aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg cgaatgtaat
    14461 agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata tcgcgagtgg
    14521 tggcggtatc ggggaaaaac agcgtcagcg acaaaccctg atcgacatgg cgagtcgctt
    14581 ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcatcctga tacagcgcca
    14641 gattttcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg ccctctttgc
    14701 gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg atataagaga
    14761 tagaaccggt tggcggcacc gcctgaagat tctggttgta aatgccgtag cgcatcacgt
    14821 cgtcgcgcag ttgcgcccac atctcacggg taggtaacgt aataccgctg cgggcaaaca
    14881 gttcgccaac tttcgccgtt ttcggctgcc agttaccttg cagatattgg ctaaaatatt
    14941 caccactggc ataacgtgac tgtttgaacc cggcgaaggt ttcaccgcgt tcgcgcgcca
    15001 gcaacatcga ggtacgcagt gcgtgccagg tgatggcata gaaatagaga ttggtgaaat
    15061 ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg tgtaaattca
    15121 tctgccccag tccgatggcg tgtgaggcgg catttccggc ttcgatggac ggcacgctgc
    15181 ggatatgact catatctgac accgccgtta aaccgcgcac ggcagtctct accgtgcggg
    15241 caaaatcggg ggaatccatg gtgtgcgcaa tattcaacga acctaaattg caggagatat
    15301 catggcctgt gcgggcatag tcgagattct cgtcatactc tgaggcacag ttgacctgca
    15361 aaatctctga gcagagatta ctcatattta tgcgcccggc gacagggtta gcacggttta
    15421 ccgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct gccagccgct
    15481 ggaagaaatc acgggcgttg aggtattttt tgcggatgcg ttcatcggca accagttcgt
    15541 catagtgttc gctgatggcg atatcggcaa acggcttgcc ataaactcgc tcaacgtcat
    15601 agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg atatccggga
    15661 tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt tcccgttttg
    15721 tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgca cctgccccct
    15781 gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc atcaccggaa
    15841 ttacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttct cgcagattcg
    15901 acagcaaaaa tgccacgccg ccgccgcgtt tcgacagttg cagtgcggaa tttaccgccc
    15961 ggccaatcga ctccatattg tcttcaatac gcagcagaaa acaggaaacc agttcgccgc
    16021 gctgctgctt accgcagttg aggaatgtcg gcgtggctgg ctgaaagcgt cctgacagca
    16081 tttcatcggt cagttgcaac gccagcgtcg catcgccttg tgccagcgtc agcgccacca
    16141 tcgtgactcg atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc ttcaacgtat
    16201 agctggtgta aaacttccat gccccgagga atgtctggaa acgaaaaccg ctggtgtgtg
    16261 cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg ctttcatcat
    16321 agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag gtcacactgt
    16381 tcggacgcac atgcgtcgcc ataaaggcgt caacggcctg gcggtcttta tcgaactgaa
    16441 tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa tccatcgttt
    16501 cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt tacgaacgtt
    16561 ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca cgccgcattt
    16621 ccgggcaatc acatctccgg cgcggccata cgcctcacca aagttgcgat taccagaagc
    16681 aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa ttacctatcg
    16741 tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga tgtaaggctc
    16801 gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca gacctaaacg
    16861 ttcgataaaa cgctgcgtgt tttcggagct gctggagaag tagacgagct ggctcatgca
    16921 ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa accagaccag
    16981 ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg caacgcttcc
    17041 gctgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg gttttccatc
    17101 gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt tatgcgcatg
    17161 attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct
    17221 gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat atatagcaaa
    17281 tagtgatcga aattacccta gatatgagcg tgacggggga gggggatttt tgtgattcac
    17341 caggcaaaaa gaaaccccga agacaggctt cggggttaaa gacgcgtatt tattatcatt
    17401 tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagctgc
    17461 tgtacccacg ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc
    17521 gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt ctttcagcaa
    17581 tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc cccaggactt
    17641 caatacagat tcgaggctgt cagctaattg attgacatca ttctggatat cctgcacgcc
    17701 atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg
    17761 taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt tatggacttt
    17821 tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat aataacgagg
    17881 atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac
    17941 tgtcgatgtg gtaacccaga aagcttatgg ttaccgccgt ggggaaaatt atgtctatgt
    18001 taatcgcgaa gcgcgaatgg gacgtaccgc actggtaatt catccaacat taaaagaacg
    18061 tagttcgaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt atcaacaatt
    18121 tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg gcttcagttc
    18181 gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga
    18241 ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa tggctgactt
    18301 taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa
    18361 caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca
    18421 agatcgccac atacaccggt gactggaacg tcacataagc caggatatca gccagatttt
    18481 ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaatagatcg cggtacatac
    18541 cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa taaaaagact
    18601 gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa
    18661 caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc
    18721 taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag tggatctcat
    18781 tgccatctgc attttcgcat agcgtatatc gctggctatg actattcaat tagtgaaaaa
    18841 tttttattta gtcagttttt gtagataaaa tattccgtaa cggctcgttt tttgtacatt
    18901 gtggattttt acatgctgat atgatttatt ttcaattaaa tcaattagat atgaccttgc
    18961 ctttaatttc actaatgaaa gtttgggggt gaggtaacgc tataagcgta gagaattcgg
    19021 tgtatgtcgt gaccgtgtca tttacagcat ttttgatgcc agaaaatgta cattaacagc
    19081 agaaagcacc agtg
//
LOCUS       DAJVYM010000074        18974 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVYM010000074 DAJVYM010000000
VERSION     DAJVYM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.074"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..167
                     /locus_tag="OQS60_002373"
     CDS             <1..167
                     /locus_tag="OQS60_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS2388104.1"
                     /translation="ILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQA
                     VSWANDNLRR"
     gene            172..561
                     /gene="csgE"
                     /locus_tag="OQS60_002374"
     CDS             172..561
                     /gene="csgE"
                     /locus_tag="OQS60_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS2388105.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            586..1002
                     /gene="csgF"
                     /locus_tag="OQS60_002375"
     CDS             586..1002
                     /gene="csgF"
                     /locus_tag="OQS60_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS2388106.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1029..1862
                     /gene="csgG"
                     /locus_tag="OQS60_002376"
     CDS             1029..1862
                     /gene="csgG"
                     /locus_tag="OQS60_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCS2388107.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1927..2418)
                     /locus_tag="OQS60_002377"
     CDS             complement(1927..2418)
                     /locus_tag="OQS60_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS2388108.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2520..3074)
                     /gene="ycdY"
                     /locus_tag="OQS60_002378"
     CDS             complement(2520..3074)
                     /gene="ycdY"
                     /locus_tag="OQS60_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS2388109.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3098..3835)
                     /gene="ycdX"
                     /locus_tag="OQS60_002379"
     CDS             complement(3098..3835)
                     /gene="ycdX"
                     /locus_tag="OQS60_002379"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS2388110.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3890..4828)
                     /gene="ghrA"
                     /locus_tag="OQS60_002380"
     CDS             complement(3890..4828)
                     /gene="ghrA"
                     /locus_tag="OQS60_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS2388111.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5062..5149
                     /locus_tag="OQS60_002381"
     tRNA            5062..5149
                     /locus_tag="OQS60_002381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5096..5098,aa:Ser,seq:gga)
     gene            5194..5330
                     /locus_tag="OQS60_002382"
     ncRNA           5194..5330
                     /ncRNA_class="other"
                     /locus_tag="OQS60_002382"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5421..6107)
                     /locus_tag="OQS60_002383"
     CDS             complement(5421..6107)
                     /locus_tag="OQS60_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS2388112.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(6688..7476)
                     /gene="phoH"
                     /locus_tag="OQS60_002384"
     CDS             complement(6688..7476)
                     /gene="phoH"
                     /locus_tag="OQS60_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS2388113.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8097..9368)
                     /gene="efeB"
                     /locus_tag="OQS60_002385"
     CDS             complement(8097..9368)
                     /gene="efeB"
                     /locus_tag="OQS60_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS2388114.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9374..10501)
                     /gene="efeO"
                     /locus_tag="OQS60_002386"
     CDS             complement(9374..10501)
                     /gene="efeO"
                     /locus_tag="OQS60_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS2388115.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10559..11389)
                     /locus_tag="OQS60_002387"
     CDS             complement(10559..11389)
                     /locus_tag="OQS60_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS2388116.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12055..13563)
                     /gene="putP"
                     /locus_tag="OQS60_002388"
     CDS             complement(12055..13563)
                     /gene="putP"
                     /locus_tag="OQS60_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS2388117.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            13985..17947
                     /gene="putA"
                     /locus_tag="OQS60_002389"
     CDS             13985..17947
                     /gene="putA"
                     /locus_tag="OQS60_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS2388118.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17987..18625)
                     /gene="rutR"
                     /locus_tag="OQS60_002390"
     CDS             complement(17987..18625)
                     /gene="rutR"
                     /locus_tag="OQS60_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS2388119.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         4820 a         5066 c         4619 g         4469 t
ORIGIN      
        1 agatcctgaa taaactgcgt atcggcgcgt ctaataacga gatcgctcgt tcgttgttca
       61 tcagcgaaaa tacggttaaa acgcatcttt ataatctttt caagaagata gccgtaaaaa
      121 accggacaca agcggtttcc tgggcaaacg ataacctcag gcgataaagc catgaaacgt
      181 tatttacgct ggattgtggc ggcagaattt ctgttcgccg cagggaatct tcacgccgtt
      241 gaggtagaag tcccgggatt gctaactgac catactgttt catctattgg ccatgatttt
      301 taccgagcct ttagtgataa atgggaaagt gactatacgg gtaacttaac gattaatgaa
      361 aggcccagtg cacgatgggg aagctggatc actataacgg tcaatcagga cgttattttc
      421 cagacttttt tatttccgtt gaaaagagac ttcgagaaaa ctgtcgtctt tgcactgatt
      481 caaactgaag aagcactaaa tcgtcgccag ataaatcagg cgttattaag tacgggcgat
      541 ttggcgcatg atgaattcta aataaaaaat tgttcggagg ctgcaatgcg tgtcaaacat
      601 gcagtagttc tactcatgct tatttcgcca ttaagttggg ctggaaccat gactttccag
      661 ttccgtaatc caaactttgg tggtaaccca aataatggcg cttttttatt aaatagcgct
      721 caggcccaaa actcttataa agatccgagc tataacgatg actttggtat tgaaacaccc
      781 tcagcgttag ataactttac tcaggccatc cagtcacaaa ttttaggtgg gctactgtcg
      841 aatattaata ccggtaaacc gggccgcatg gtgaccaacg attatattgt tgatattgct
      901 aaccgcgatg gtcaattgca gttgaacgtg acagatcgta aaaccggaca aacctcgacc
      961 atccaggttt cgggtttaca aaataactca accgattttt aagccccagc ttcataagga
     1021 aaataatcat gcagcgctta tttcttttgg ttgccgtcat gttactgagc ggatgcttaa
     1081 ccgccccgcc taaagaagcc gccagaccga cattaatgcc tcgagctcag agctacaaag
     1141 atttgaccca tctgccagcg ccgacgggta agatctttgt ttcggtatac aacattcagg
     1201 acgaaaccgg gcaatttaaa ccctacccgg caagtaactt ctccactgct gttccgcaaa
     1261 gcgccacggc aatgctggtc acggcattga aagattctcg ctggtttata ccgctggagc
     1321 gtcagagttt acaaaacctg cttaacgagc gcaagattat tcgtgcggca caagaaaacg
     1381 gcacagttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca aatatcatgg
     1441 ttgaaggttc gattatcggt tatgaaagca acgttaaatc gggcggtgtt ggggcaagat
     1501 attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg aacctgcgtg
     1561 tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag acgatacttt
     1621 cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg ctggaagggg
     1681 aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct atcgaaacag
     1741 gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg cagaacaaag
     1801 cagaacggca gaatgacatt ctggttaaat accgccatat gtcggttcca ccagaatcct
     1861 gacagataaa aatgccggaa ttaataatcc ggcattttac tgtttaagcg aaagcgtgag
     1921 tgcccctcac gctttatttt tgatttcctg ctcacggtga gccgtctttg cactacgcgc
     1981 cgccagccac aggccgctgt ttttcattgc gtaaccaaat accagcccaa gcgccagcga
     2041 aggtaacacc agtttccagt caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt
     2101 ccccggaaca aatgaaagca gcagctgttt ggcctgaata cacatcagaa acgccacaat
     2161 accggttatg acataaccga gaatttccag atgtggtgcc agtgcactac cgtaaataat
     2221 gaccatcgcc cacaccacgc cactaagcag ggttgcagcg gagatcgcca gccctttcag
     2281 cccaccttgc gggcaggcaa agtaggccgt acagcccaag aagcccgccc agcttagtaa
     2341 gcccagggaa acagccaccc atccccagat accggagaga atgcctgttg tgattgcaat
     2401 agagagaagt atgttcatgc cgcgcatgat agcagaaaac gcggccatga atgagatcga
     2461 gcacacattt tatgcaacaa caaattatct gttgcagtta tgagtgacga tgatcacatt
     2521 tactcttcag aatcttcttc cagctcgtcc cacattgcac taatggcatc acgggttagc
     2581 ggtgccatgg tgcgccagaa aggcgtggtt gcatgggcct ccactttgcc gaggaacgca
     2641 ccacaccagg gtaacagata ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc
     2701 gttgactgat cttccagcca ggaagccgca agcagcaatg tgccgatgtg atcggctggc
     2761 gtatccgcca gtggcatccc tcgttcggaa agaaaagcgc gcacttccgc ttccgttacg
     2821 tcctcaaccc atgcgctacg atatggcggc actgcacatt catcgccgat aaacaacgca
     2881 ttgtaatcgg cagagacttg cgccatatca cagcttttct gtaaacgcgt cagtaactca
     2941 tcctgctcca gtggccagtt cgcagccagt ttcccctcac gaatcagggt aaacagcggc
     3001 accagtaaag gatcttgcgg ttggcggtaa tagagcgaac ccagcacacg acagaggata
     3061 gaaaactcgt tcattaatat atttccatta ctagtaatta aaggtctgca aattccgcaa
     3121 tcggtgccat accgcgagat tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa
     3181 tgcgctctgg cggaaaatct accgcgtcga ggattttaag acactcttca aattccccca
     3241 tggtaaacgc ggtgtgagaa tccgagccta atgccaccca acctccagca tcacgtaccg
     3301 ctgcggctac cgcacgacag ttgtcttcac tgcccttacg tgagtgtaaa aatgaagaat
     3361 tattgatttc cagcgccacc tgatgtttcg cggctgcttc agcaacggct ttcacatcta
     3421 tttcatattt gggatttccg ggatggctta ttatatgcac attgccgctg gcgatagtgg
     3481 cgatcatcgc ttgtgtattg gtcgctttgt catgtggtgc aaaaaccggc tcatgaaaac
     3541 cggcaataat cagatccagc gagtcaaaca ttttaccgct gcaatcaatt tcaccatcaa
     3601 catttttaat gttagcttcg atgccgcgca ggatccctac cccatcaacc actcgcggcc
     3661 agatacgcat gttaatgaag tgccagtgat gcggagcatc ttccatatcc gggccatgat
     3721 cggtgatcgc aaaaagttta atgccctttt gtttggcctg ggcaatgtaa tcactcaatg
     3781 tgctatatgc atgtgtgctg gcaacggtat gcatatgaag gtcgacggga tacataactc
     3841 tctcctgtat tcaattcccg ccaaggatag caggaatcct gatgctttat tagtagccgc
     3901 gtgcgcggtc gacttgcccg cagaccctct cccctttttc gagctgggca atggtgcgag
     3961 aaatgtactc cacagcttca gcgggacggg taatcgcggc gacatgtggt gttatcgtca
     4021 cgcgtggatg ttgccagagc ggactttcag gcggtaaggg ttcacgatta aaaacatcca
     4081 acattgcgcc tttaactttg ccgctatcca gcgccgcgag caggtcatct tccacaacat
     4141 gaacaccacg cgccaggttg aggagatacg cgccatccgg taatttttcg agtaattgtt
     4201 gattaataat gccgacggtt tcaggggtat tcggtaacaa attaatcaat acccgacatt
     4261 ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt
     4321 tacgggttcg actccagcaa cgcagcggaa agcgccaggt ttgcagactc tgagcaactt
     4381 tactgcccag tacgcctgcg cccaaaatgc cgatggtaaa atcttcccga tgatattcag
     4441 gcagcggttg ccaatgcgaa ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc
     4501 aatgcagcac ctgactgaca gcatattcct gcatttgctc gcccataccg gtatcttcca
     4561 ggcgaaaaag tggaacagaa gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa
     4621 tagaatcaac accggccccg agtgcgaaca ccgctttaag atcgcgccct gccagcattt
     4681 caacaggagg atgccagact aaagcataat cagcagagtc attatctccg cttttccatg
     4741 ctctgactct tgcctgagga atagctttgc gcagtgcctc aatccaccat tgggtatcga
     4801 acgttgggtg ataaaagatg atatccatac tgactcccga aaagcgttgt gcgaaattta
     4861 ttcgcactta tcgttatgat ctacaaaggc caccagcata acaaatccgt ggtcggtggc
     4921 aaaaaaagca gatttcgctt attaaaacca cacattgatt gaagtttgaa taaacgcgcg
     4981 attttttcaa aaagtttgtt gacctcaggt catgatttcc ctaaattagc gcccgttcca
     5041 gcaagacagg aacgacaatt tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa
     5101 gtgtgtatac ggcaacgtat cgggggttcg aatccccccc tcaccgccat atttaaagaa
     5161 gagctcgtac gaaagtacgg gctttttttt cgtatattgc acacaccggg gggatgagaa
     5221 gccccgaccg gggttcgaca actggcgaca gccagttgga cagaccgtga acgcagtgag
     5281 cgggctgccc gcagggcgag cgaagcgagt caatcccccc ctcaccgcca tctttaaaga
     5341 agagctcgta cgaaggtacg ggcttttttt cgtatattgc acacaccggg gggatgagaa
     5401 gccccgaccg ggatacttag ttaatgcacc ggttcactct ttgtcgccgc gactaatggg
     5461 cgaattttgt cacgagtctc ccagaccgga ggaatttcct ccaatttagt gacagaagct
     5521 cgtacaaaag gcacccgtgc aagagtcata attgtatcca accaacggtc acgaataatg
     5581 cgatccgccc tttcatgact cgggtcatcc agctcaataa cacagactat tttattgttc
     5641 ttctttgaaa ccagaacata atccattgcc atgccattat aaattgcaaa gaaacgcttt
     5701 ctttcttctt cgctaaactc aggttgttct ggaaagttga tcaagcgccc cagagagacc
     5761 tggcaatgga tatgacaata atgtccaaac catttttcca gattatcgaa atacattttt
     5821 tcacaacgcg tgattaacca ctcttttctt tccaataaaa taccttcggc aaccattccc
     5881 tgaaataact catctcgctc ttgccaacga gagtcacgat aggttttctg cggtatatag
     5941 ctccttgtcg acgccttagc ggcaggggaa gttgcagatg aagggacggg ttgcgattta
     6001 gcgatgatta ccgatgaggc aacttttcgg agtgtcaatt ttctggcaac agcaaaaccg
     6061 gcgataaatg caattgtcaa acaagttaca aaagccataa agctcataat taattttcac
     6121 ctatccatgt tgattatatt tcattaccaa tgtaactcgc tgcagcgtgt tacatgccaa
     6181 caatgcaacg atcaatatat agttagtatc atgttttgat tattacaacc atccctattt
     6241 agccattgcg gcttgccttc cacaaaaaaa taacaacaca caatattagc aaagaattaa
     6301 ttaagattaa ccttacattt ataatattat ccctaaagaa taaatcagag gaatatattc
     6361 ggtgttaaac agtcagtcaa agtatagccc attgttgatt aaacctcata agcaaaattg
     6421 gcaccagatc tcctgtacag gttgaatggc cgaaaaaaga taggcagatg ctcatttttt
     6481 tggcttctgg aagaaaaact ctccgcgctg gcagtattac actggtctga tgctatgacg
     6541 aaaaatgcct ggtaagagta tctaaaccag actccagttc agcttatcaa cacgctcaaa
     6601 gtcgcccccc tgaacgctaa cctgaagtga aaatctgttg aaataaaaca aagcccggtt
     6661 cgcccgggct ctgcaccgat aacacactta actgtaggca tgcagcgtac gttggcaaag
     6721 tgccgaacgt acgcagtcct ctttaccgaa ccggacgatc ccaaccattt catcttcttc
     6781 gaaacgttcc agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt
     6841 gatatcaccg ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat
     6901 ttgcgcggca gtcacattct gcgcctcgtc aagaatgacg actgcatttt caaaggtacg
     6961 tccacgcata taggcgaacg gcgcaatttc caccttccct atttccggtc gcaggcagta
     7021 ctgcataaag gaagccccta agcgccggac cagcacgtcg tagaccgggc gaaaataggg
     7081 agcaaacttt tctgcgatat ctccaggtaa gaagccaaga tcttcatcgg cttgcagaac
     7141 tggacgggtg acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc
     7201 tgcgctgatc caggtttttc cgcccccggc ttcgcccgtg gcgaatatca gctgcttact
     7261 ctcaatagcc ttcagatagt gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc
     7321 gcgactgtcg cgggccatac caatggcttc tacgccgccc atctgcacaa gcgaggtgac
     7381 cgattcttct tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc
     7441 gcgacgagct ttgatcactg ctttttgtct tcccatagag agcaccttga gttgtttgta
     7501 ttcatcacac gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc
     7561 ttgtaagcca tagtttgctt ttggataaaa tgccgaaaaa cggctacgcg caccgtttac
     7621 ggcgtcggta acacatgaaa agaaaggatg aggttgaaaa tgcaaagtga cgagatgact
     7681 accggaggag aaaactccgc gagtggtggc gcgttgatta tctaaaacat gtccagtaca
     7741 ggacgttacc atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc
     7801 tacttaataa gtacaacaga tctcgcattt attgcaacaa tatatttact tatatttaac
     7861 tataaaacac tatttcagtg acattagttt ctactggaaa gatgacagag tgatgacagt
     7921 gatgaaaaaa gctgtgtgct ttcagcagga tttgcatcag gcatcagtgc acgatcgccg
     7981 gatgcggcgt gaacgcctta tccagcctac caaacccgca ctgattgtag gcatgataag
     8041 acgcgcaagc gtcgcatcag gcatctgcgc acaaatgcct tatccgccta aaaacattaa
     8101 acccgcaata acgcgcttcc gagataatcg ttcgcgtcct tcactcccgg cagcgaaaaa
     8161 aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt gagccttttt
     8221 tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag caaccccata
     8281 tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg cagcatcagg
     8341 ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc cagcgcaatc
     8401 accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg cattcccagc
     8461 ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag cggcgttctg
     8521 tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc accgattgtc
     8581 cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa cttatcattc
     8641 tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg cgtctctttg
     8701 cctttactac gcgacacgtg atcggaaata aacccttccc gcttccagcg cacactgagc
     8761 aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga taaccgtgtc ctgggtgttg
     8821 gcgcaaatct gtagcaacac atcaccatga cataacgccg catccagcga gtcgttgggg
     8881 aaacgcgtca tcttctgcag cttttttggc atctgtggcg caaggccaaa gcgctcatca
     8941 aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat gtagccgcca
     9001 agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg tttctggtgc tgctccgccc
     9061 tgagtcagaa aagcaaaacg ctgagtcaac aagcgaaaca accgctcaag atcggcttta
     9121 tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc
     9181 cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg tgaaagcgtg
     9241 cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc cagcgccagc
     9301 gcaccaatcc ctttcagtaa acgtcggcgt gacggttcat tcacgccgtt ttcatcttta
     9361 tactgcataa cgcttaatcc agtcccagca caccgcgaag ttgcgccaga tcttccgcca
     9421 gcgcagtaat cggtcctttc agtgcattcc ggtcggcatc ggtcaatttg tcgtaggttt
     9481 caaaaccgtc tttagtacgg tatttcgcca gaatggtatc gaccttttta aagttggcat
     9541 cgacttttgc cagcagttcc gggttggctt tttgcagttg tggacgcagc aaatcgacaa
     9601 ttttctgcga gccttcaacg ttagcctgga aatcccacag atcggtgtgg ctgtagcgat
     9661 cttcttcacc gctaattttg ctggctgcca cttcctcaat cagtccggct gcgccgccga
     9721 ccacttttga aggtgggaaa gccagttcac tgatgcgttt ttgcaaatcg accacatctg
     9781 tataaagctg ctcagcgtac tgatccatcc ctttggtggt gttgtcgcca aacaatgctt
     9841 tttccagacg gtggaaacca gtgaattttg ggtcggcggc tttttgctcg taatcatctt
     9901 cacgggcgtc aatgctgcca tccagatcgg agaacagttc agcaatcggt tcaatgcgct
     9961 catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt ttcgatatcg cctgctttaa
    10021 tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt ggtttccgcc atgacatacg
    10081 ctttatatgc agtaattgca ccaccaagac ttaacagcgc atcactttgc gccgcatccg
    10141 ccgttgcctc acctttgacg atcaacttcc ctttcgggtt agtcagcaga ccgcaggtca
    10201 tatcgtattc gccaggctgt aaattcgccg tcattttctg gctaaaacca ggggcgatat
    10261 tttcccgctc ttccaccacc atcacgcctt tgaggatctc ccactccagc gccttctggc
    10321 tatggttctg aataatgaac tgtgttttcc cggcgttaac cgtaatggtc atcggttcgc
    10381 actgcttatc cgtcacggtc actttgacct gcggcacatc agcggcgtta gccataaaag
    10441 cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt acggcggaag ttaatggtca
    10501 tgacgagact atccctttaa agtatgttgt aactaagcaa gagtttgcgt cgtatttgtt
    10561 acgcggagcg agacgctgtc gcccctgcgc gtggtggcag agcaaatgcc accagcgccg
    10621 ggatgagata aataaaccag acggcgactt cgctgacgct cggtgcttcc tgatagccaa
    10681 aaatcccttc catcagcgtg ccaaacagcg agtgagttga gagcactgcg ctcatatcga
    10741 aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg aaatgcgcga atggcaccag
    10801 cagccagccc tgcggcgacg aagagaataa acaggctggt ccatttaaaa aatgcaccaa
    10861 gattgaggcg aataccgccc cagtagagca ggaagcctag caccacggca gtagcaagac
    10921 cgagcattgc acccagcggc ggccagatcc cgacatcttg ttgaaatgcc gccagcagga
    10981 aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa gaccatcatc accagcgccc
    11041 agccatgatg atttccacgc tgcaatgcgc tatcgactgc ctgctccagt tgtactttga
    11101 cgttgcgcga cactttgcgc atccagaaaa ccatccaggt aaggatcacc acggcgatca
    11161 ccgccacgat accttcaaac agttcctgtt ctttttgcgg aaattcgccg gtggtttcgt
    11221 taatgaagat acccaagccc aggcacaacg cagcggcaag caacacgcca atccacatca
    11281 cgccaatcca tcggcctcgc tgggtacgct taagatagct ggcaatcaaa ctgacaatca
    11341 gcgcggcttc aagtccttcg cgcaacataa tgagaaacgg aacaaacatg ccacccaccc
    11401 ttaaacgttt ttctgagtca atccatgcaa agaaaagtaa atcgcagtga tagcgattat
    11461 cattacggca aagaaaaata caagcggaat gtttataaaa tgatgttgga atgtaaacaa
    11521 agagaggtaa taccagggga tataaacgat tattccaggt acaaagatgg gattgtttac
    11581 aacgagccat gtgttcttgc cagatgccgc gtaaacgcct tatccagccg acaaaactgt
    11641 ccagactcaa tgcatcacag cttccatgta aaactgataa gcgggtgtat caagcaattt
    11701 cgtccatccg cgattatcaa atctaacttt ttgcccggaa cataataaca gcgcgggtgt
    11761 tggtggcgtc aaaatcgctg aaacgttccc cgaaggccgg ggcagcccac atggatgtgg
    11821 gctgagggcg cgtcttacag gacgttacct cgcgcccgac ccggtagccg aaggggataa
    11881 gctgaaggta ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc agggggcggc
    11941 ggcgactggc cgccccctgc gcgctccctg caccattcaa tttatatggc gaggcacatt
    12001 aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag tcccttagtt
    12061 ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg caaagcgttt
    12121 ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc cgatactgcc
    12181 gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct tccagacgat
    12241 aactgtcagc gcaccgatga tcatccccgc cagcgcaccg ttgcgcgtca tgcgtgacca
    12301 catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc acgcgtagct
    12361 cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca ccagcgccac
    12421 caccagcacc atcacacgtc ctacccacac cagctctttc tggctggcct gtttacgcag
    12481 aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagct ggcaacttaa
    12541 ggttgacatt accgccgcca gaatcgccga cagcagaatc ccggcaatcc acgggttaaa
    12601 cagaatttgc gccagttcga taaacacgcg ttcagcgttc tggtttaccg cacctgctac
    12661 cgccggatgc tcgttaaagt aagcgatacc aaagaagcca acagccaccg cccctgccag
    12721 gcaaaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt ggtgagaatc
    12781 tgccgccata aaacgcgcca ggatgtgcgg ctgcccgaag taacccagcc cccaacccat
    12841 caatgagata atggcaacaa agttcagccc tttgagcata tccacgtttt cgatgctctt
    12901 ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga cgataaccgg
    12961 cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc agctcaccgc
    13021 gaggaaaccg ccaataaagg tgtaaaggat cgtcgacgca gctcccgcca acagagccgt
    13081 ttcgtaactc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa tgcccgaagc
    13141 gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca aaatgcggct
    13201 tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat tattgtattc
    13261 ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc ctaatgtcag
    13321 gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag gcaaccccat
    13381 taacagccag ccgctcatat ccgacgcacc cgccgataat gccgtcacga atggcccaag
    13441 actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg cgataaaccc
    13501 aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg tgctaatagc
    13561 catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt atatctgcca
    13621 ccggaacggg gtaacagagt ttatgtttta ccagggcgac cgtatcctgc cggaagcgct
    13681 ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc agcgacacta
    13741 tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa aaaggttaac
    13801 tgccgcagag aaaaagtctg agttattttt taatcactgt catatcgatt tcttttatta
    13861 acatttcatt catttttaag cttgctacgc aagtcacatt taacatggtt gcacaaagtt
    13921 gcaacatcat ggatatttca cgataacgtt aagttgcacc tttcagaaca acaggagtaa
    13981 tggcatggga accaccacca tgggggttaa gctggacgac gcgacgcgtg agcgtattaa
    14041 gtctgccgcg acacgtatcg atcgcacacc acactggtta attaagcagg cgattttttc
    14101 ttatctcgaa caactggaaa acagcgatac tctgccggag ctacctgcgc tgctttctgg
    14161 cgcggccaat gagagcgatg aagcaccgac tccggcagag gaaccacacc agccattcct
    14221 cgactttgcc gagcaaatat tgccccagtc ggtttcccgc gccgcgatca ccgcggccta
    14281 tcgccgcccg gaaaccgaag cggtttctat gctgctggaa caagcccgcc tgccgcagcc
    14341 agttgctgaa caggcgcaca aactggcgta tcaactggcc gataaactgc gtaatcaaaa
    14401 aaatgccagt ggtcgtgcag gaatggtcca ggggttattg caggagtttt cgctgtcatc
    14461 gcaggaaggc gtggcgctga tgtgtctggc agaagcgttg ttgcgtattc ccgacaaagc
    14521 cacccgcgac gcgttaattc gcgacaaaat cagcaacggt aactggcagt cacacattgg
    14581 tcgtagcccg tcactgtttg ttaatgccgc tacctggggg ctgctgttta ctggcaaact
    14641 ggtttccacc cataacgaag ccagcctctc ccgctcgctg aaccgcatta tcggtaaaag
    14701 cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg cgcctgatgg gcgagcagtt
    14761 cgtcacgggt gaaaccatcg cggaagcgtt agccaatgcc cgcaagctgg aagagaaagg
    14821 tttccgttac tcttacgata tgctgggcga agctgcactg actgccgcag acgcacaggc
    14881 gtatatggtt tcctatcagc aggcgattca cgccattggt aaagcgtcta acggtcgtgg
    14941 catctatgaa ggtccgggca tttcaatcaa actgtcggcg ctgcatccgc gctacagccg
    15001 cgcccagtat gaccgggtaa tggaagagct ttacccgcgt ctgaaatcac tcaccctgct
    15061 ggcgcgtcag tatgatattg gtattaacat cgatgccgaa gaggccgatc gtctggagat
    15121 ctccctcgat ctgctggaaa atctctgttt cgagccggaa ctggcaggct ggaacggcat
    15181 cggttttgtt attcaggctt atcaaaaacg ctgcccgttg gtgatcgatt acctggttga
    15241 tctcgccacc cgcagccgtc gccgtctgat gattcgcctg gtgaaaggcg cgtactggga
    15301 tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc tatccggttt atacccgcaa
    15361 ggtgtatacc gacgtttctt atctcgcctg tgcgaaaaag ctgctggcgg tgccgaatct
    15421 aatctacccg cagttcgcga cgcacaacgc ccatacgctg gcagcgattt atcaactggc
    15481 aggtcagaac tactatccgg gtcagtacga gttccagtgc ctgcatggta tgggcgagcc
    15541 actgtacgag caggtcaccg ggaaagttgc cgacggtaaa cttaaccgac cgtgtcgtat
    15601 ttatgccccg gttggcacac atgaaacgct gctggcgtat ctggtgcgtc gcctgctgga
    15661 aaacggtgct aacacctcgt ttgttaaccg tattgccgac acctctttgc cactggatga
    15721 actggtcgcc gatccggtca ctgctgtaga aaaactggcg caacaggaag ggcaaactgg
    15781 attaccgcat ccgaaaattc ccctgccgcg cgatctttac ggtcacgggc gcgacaactc
    15841 ggcagggctg gatctcgcta acgaacaccg tctggcctcg ctctcctctg ccctgctcaa
    15901 tagtgcactg caaaaatggc aggccttgcc aatgctggaa caaccggtag cggcaggtga
    15961 gatgtcgccc gttattaacc ctgcggaacc gaaagatatt gtgggctttg tgcgtgaagc
    16021 cacgccacgt gaagtagaac aggcgctgga aagtgcggtt aataacgcgc caatctggtt
    16081 tgccacgcct ccggctgaac gcgcggcgat tttgcatcgc gctgccgtgc taatggaaag
    16141 ccagatgcag caactgattg gtattctggt gcgtgaagcc ggaaaaacct tcagtaacgc
    16201 cattgccgaa gtgcgcgaag cggtcgattt tctccactac tacgccggac aggtgcggga
    16261 tgatttcgct aacgaaaccc accgtccatt agggcctgtg gtgtgtatca gtccgtggaa
    16321 cttcccgctg gctattttca ccgggcagat cgctgccgca ctggcggcag gtaacagcgt
    16381 gctggcaaaa ccggcagaac aaacgccgct gattgccgcg caagggatcg ccattttgct
    16441 ggaagcgggt gtaccccctg gcgtggtgca attgctgcca ggtcaggggg aaaccgtggg
    16501 cgcgcaactg acgggtgatg atcgcgtgcg cggggtgatg tttaccggtt caaccgaagt
    16561 cgctacgtta ctgcagcgca atatcgccag ccgcctggac gctcagggtc gccctattcc
    16621 gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc gattcttcag cactgaccga
    16681 acaagtcgtc gtggatgtgc tggcctcggc gttcgacagt gcgggtcagc gttgttcggc
    16741 actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac acgttgaaaa tgctgcgcgg
    16801 tgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg accaccgata tcggtccggt
    16861 gattgatagc gaagcgaaag ccaatattga gcgccatatt cagaccatgc gtagcaaagg
    16921 ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat gcccgtgaat ggcaaagcgg
    16981 cacctttgtc gccccgacgc tgatcgaact ggatgacttt gccgaattgc acaaagaggt
    17041 ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac cagctaccag agctgatcga
    17101 gcagattaac gcttccggct atggtctgac gcttggtgtc catacgcgta ttgatgaaac
    17161 catcgcccag gtcactggct cggcccatgt tggtaacctg tatgttaacc gtaatatggt
    17221 gggcgcagtg gttggtgtgc agccgttcgg cggcgaaggg ttgtccggta ccgggccgaa
    17281 agcaggcggt ccgctctatc tctaccgtct gctggcgaat cgcccggaaa gtgcgctggc
    17341 agtgacgctc gcgcgtcagg atgcagagta tccggtcgat gcacagttga aagccgcatt
    17401 gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat cgtccagaat tgcaggcgtt
    17461 atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa cgattgctgc cggggccgac
    17521 gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc gtgttgtgta ttgccgatga
    17581 tgagcaggat gcgctgactc agctcgccgc cgtgctggcg gtgggcagcc aggtactgtg
    17641 gccggatgac gcgctgcatc gtcagctagt aaaggcattg ccatcggcag tcagcgaacg
    17701 tattcaactg gcgaaagcgg aaaatataac cgctcaaccc tttgatgcgg tgatcttcca
    17761 cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt gctgcgcggg atggcgcaat
    17821 tgtttcggtg cagggttttg cccgtggcga aagcaatatc cttctggaac ggctgtatat
    17881 cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
    17941 cggttaaaca ggtagccgga ggatgttaca acctcctccg gcatctttaa cgtggtcgaa
    18001 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat atctcatcgc
    18061 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
    18121 aagcccaaat catgaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
    18181 cccaaccggc aatcagcgca gatttctcat caattaatgc cttcaaatcg cccgtcagtt
    18241 catccattaa cagcggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
    18301 gataatcgcg ggagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcggag
    18361 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
    18421 cggcaatata cagcgcctct tttgacggaa agtaatacag cagattggtt tttgaaacac
    18481 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
    18541 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
    18601 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggc aacaggatag
    18661 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacagatg catgttttat
    18721 gtgcaactgt tttgaccgtt tagtccactt tttatcagat attttagata acgtaatatt
    18781 aacatattaa taacattaac tttttaaaaa ctggcatccg ctttgcaaac aagccaatac
    18841 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
    18901 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
    18961 tcgacccacg cgcc
//
LOCUS       DAJVYM010000075        18529 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVYM010000075 DAJVYM010000000
VERSION     DAJVYM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.075"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..664
                     /locus_tag="OQS60_002391"
     CDS             <1..664
                     /locus_tag="OQS60_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCS2388120.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            734..1510
                     /gene="yaaA"
                     /locus_tag="OQS60_002392"
     CDS             734..1510
                     /gene="yaaA"
                     /locus_tag="OQS60_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS2388121.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1663..1959)
                     /locus_tag="OQS60_002393"
     CDS             complement(1663..1959)
                     /locus_tag="OQS60_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS2388122.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OQS60_002394"
     CDS             complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OQS60_002394"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS2388123.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OQS60_002395"
     CDS             complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OQS60_002395"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS2388124.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OQS60_002396"
     CDS             complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OQS60_002396"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS2388125.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6883..7002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OQS60_002397"
     CDS             complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OQS60_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS2388126.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7217..7903)
                     /locus_tag="OQS60_002398"
     CDS             complement(7217..7903)
                     /locus_tag="OQS60_002398"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS2388127.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8303..8443)
                     /locus_tag="OQS60_002399"
     CDS             complement(8303..8443)
                     /locus_tag="OQS60_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388128.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8539..9255
                     /gene="arcA"
                     /locus_tag="OQS60_002400"
     CDS             8539..9255
                     /gene="arcA"
                     /locus_tag="OQS60_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS2388129.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OQS60_002401"
     CDS             complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OQS60_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS2388130.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OQS60_002402"
     CDS             complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OQS60_002402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS2388131.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OQS60_002403"
     CDS             complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OQS60_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS2388132.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OQS60_002404"
     CDS             complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OQS60_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS2388133.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMLWRQ"
     gene            13535..14404
                     /gene="robA"
                     /locus_tag="OQS60_002405"
     CDS             13535..14404
                     /gene="robA"
                     /locus_tag="OQS60_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS2388134.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OQS60_002406"
     CDS             complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OQS60_002406"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS2388135.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15100..15621
                     /gene="yjjX"
                     /locus_tag="OQS60_002407"
     CDS             15100..15621
                     /gene="yjjX"
                     /locus_tag="OQS60_002407"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS2388136.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OQS60_002408"
     CDS             complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OQS60_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS2388137.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OQS60_002409"
     CDS             complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OQS60_002409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS2388138.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18270..>18529
                     /locus_tag="OQS60_002410"
     CDS             18270..>18529
                     /locus_tag="OQS60_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS2388139.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVR"
BASE COUNT         4466 a         5063 c         4514 g         4486 t
ORIGIN      
        1 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
       61 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
      121 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
      181 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
      241 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
      301 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
      361 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
      421 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
      481 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
      541 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      601 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      661 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      721 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      781 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
      841 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      901 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
      961 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     1021 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     1081 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     1141 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     1201 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     1261 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     1321 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     1381 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     1441 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1501 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1561 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1621 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1681 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1741 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1801 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
     1861 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1921 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1981 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     2041 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
     2101 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     2161 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     2221 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     2281 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     2341 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
     2401 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     2461 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2521 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2581 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2641 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2701 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2761 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2821 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
     2881 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2941 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     3001 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     3061 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     3121 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     3181 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     3241 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     3301 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     3361 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     3421 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3481 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3541 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
     3601 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
     3661 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3721 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3781 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
     3841 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
     3901 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3961 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
     4021 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
     4081 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
     4141 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
     4201 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     4261 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
     4321 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     4381 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     4441 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4501 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4561 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
     4621 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4681 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
     4741 gcagcacagg ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga
     4801 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
     4861 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
     4921 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
     4981 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     5041 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
     5101 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
     5161 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     5221 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
     5281 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
     5341 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
     5401 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     5461 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5521 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5581 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5641 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
     5701 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
     5761 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5821 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
     5881 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
     5941 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
     6001 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     6061 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     6121 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     6181 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     6241 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
     6301 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
     6361 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
     6421 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6481 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
     6541 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
     6601 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6661 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6721 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6781 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6841 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
     6901 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
     6961 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
     7021 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
     7081 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
     7141 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
     7201 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
     7261 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
     7321 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
     7381 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
     7441 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
     7501 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
     7561 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
     7621 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
     7681 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
     7741 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
     7801 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
     7861 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
     7921 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
     7981 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
     8041 tgactgtact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
     8101 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
     8161 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
     8221 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
     8281 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
     8341 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
     8401 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
     8461 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
     8521 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
     8581 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
     8641 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
     8701 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
     8761 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
     8821 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
     8881 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
     8941 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
     9001 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
     9061 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
     9121 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     9181 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt
     9241 gatctggaag attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt
     9301 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     9361 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
     9421 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg
     9481 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     9541 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     9601 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     9661 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     9721 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     9781 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     9841 aggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     9901 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
     9961 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    10021 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    10081 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    10141 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    10201 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg
    10261 atatttggcg cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    10321 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    10381 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    10441 gttttatcct cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    10501 acaagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    10561 tcggcacgtt cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact
    10621 gcaccaaaca gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt
    10681 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    10741 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    10801 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    10861 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    10921 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    10981 gttatacgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    11041 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg
    11101 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc
    11161 acatcaacag cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    11221 gtttccacca gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc
    11281 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    11341 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    11401 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    11461 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    11521 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa
    11581 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    11641 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    11701 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac
    11761 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    11821 tttgccgaat cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg
    11881 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    11941 gcctgagcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    12001 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    12061 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    12121 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    12181 gatgagtatt aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    12241 taatgtgggt atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    12301 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    12361 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    12421 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    12481 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    12541 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    12601 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga
    12661 tatcaggcaa accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    12721 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    12781 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    12841 atcgcctctg ttattgccgc cacagcatga caggaaccgc gctaatcgcg tttttcggcg
    12901 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    12961 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    13021 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    13081 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg
    13141 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    13201 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    13261 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    13321 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    13381 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    13441 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    13501 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    13561 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    13621 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    13681 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    13741 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    13801 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    13861 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    13921 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    13981 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    14041 caacgcgccg accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    14101 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    14161 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    14221 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    14281 aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    14341 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    14401 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    14461 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    14521 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    14581 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    14641 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    14701 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    14761 cagagaatcg atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc
    14821 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    14881 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    14941 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    15001 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    15061 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg
    15121 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    15181 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc
    15241 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    15301 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    15361 gtggtgattg aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct
    15421 gcggtgattc tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat
    15481 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    15541 ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat
    15601 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    15661 acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag
    15721 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    15781 acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc
    15841 gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    15901 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    15961 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    16021 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    16081 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    16141 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa
    16201 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    16261 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    16321 ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg
    16381 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    16441 ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg
    16501 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    16561 gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg
    16621 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    16681 acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt
    16741 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    16801 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    16861 tgcggcgctt tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc
    16921 gggtagaaac cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt
    16981 ccgcgttcca gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc
    17041 atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt
    17101 cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat
    17161 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    17221 tcctgaatct gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt
    17281 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta
    17341 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt
    17401 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    17461 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    17521 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    17581 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    17641 catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag
    17701 gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    17761 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    17821 tgattcatca gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    17881 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    17941 atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    18001 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    18061 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    18121 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt
    18181 gatcgaccaa ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    18241 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    18301 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    18361 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca
    18421 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    18481 ttggttacct gccgcaggaa ccgcagctga acccggaaca caccgtgcg
//
LOCUS       DAJVYM010000076        18513 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVYM010000076 DAJVYM010000000
VERSION     DAJVYM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.076"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="OQS60_002411"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="OQS60_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS2388140.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="OQS60_002412"
     CDS             735..1040
                     /locus_tag="OQS60_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388141.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="OQS60_002413"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="OQS60_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS2388142.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="OQS60_002414"
     CDS             1788..2534
                     /locus_tag="OQS60_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS2388143.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="OQS60_002415"
     CDS             2534..4630
                     /locus_tag="OQS60_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS2388144.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="OQS60_002416"
     CDS             4676..5815
                     /locus_tag="OQS60_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS2388145.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="OQS60_002417"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="OQS60_002417"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS2388146.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="OQS60_002418"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="OQS60_002418"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS2388147.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="OQS60_002419"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="OQS60_002419"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS2388148.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="OQS60_002420"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="OQS60_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS2388149.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="OQS60_002421"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="OQS60_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS2388150.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="OQS60_002422"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="OQS60_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS2388151.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="OQS60_002423"
     CDS             complement(12873..13730)
                     /locus_tag="OQS60_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS2388152.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="OQS60_002424"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="OQS60_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS2388153.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="OQS60_002425"
     CDS             complement(14122..14709)
                     /locus_tag="OQS60_002425"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS2388154.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="OQS60_002426"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="OQS60_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="OQS60_002427"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="OQS60_002427"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS2388155.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="OQS60_002428"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="OQS60_002428"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS2388156.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4912 c         4782 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAJVYM010000077        18158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVYM010000077 DAJVYM010000000
VERSION     DAJVYM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.077"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="OQS60_002429"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="OQS60_002429"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS2388157.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="OQS60_002430"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="OQS60_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS2388158.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="OQS60_002431"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="OQS60_002431"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS2388159.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="OQS60_002432"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="OQS60_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS2388160.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3341..3544)
                     /locus_tag="OQS60_002433"
     CDS             complement(3341..3544)
                     /locus_tag="OQS60_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388161.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3622..4716
                     /gene="ddlA"
                     /locus_tag="OQS60_002434"
     CDS             3622..4716
                     /gene="ddlA"
                     /locus_tag="OQS60_002434"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS2388162.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4740..4952)
                     /locus_tag="OQS60_002435"
     CDS             complement(4740..4952)
                     /locus_tag="OQS60_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS2388163.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5212..5520
                     /locus_tag="OQS60_002436"
     CDS             5212..5520
                     /locus_tag="OQS60_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS2388164.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5579..6673)
                     /gene="yaiW"
                     /locus_tag="OQS60_002437"
     CDS             complement(5579..6673)
                     /gene="yaiW"
                     /locus_tag="OQS60_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS2388165.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6686..7906)
                     /gene="sbmA"
                     /locus_tag="OQS60_002438"
     CDS             complement(6686..7906)
                     /gene="sbmA"
                     /locus_tag="OQS60_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS2388166.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8258..9415
                     /gene="ampH"
                     /locus_tag="OQS60_002439"
     CDS             8258..9415
                     /gene="ampH"
                     /locus_tag="OQS60_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS2388167.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9416..10039)
                     /gene="iprA"
                     /locus_tag="OQS60_002440"
     CDS             complement(9416..10039)
                     /gene="iprA"
                     /locus_tag="OQS60_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS2388168.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10127..13015)
                     /locus_tag="OQS60_002441"
     CDS             complement(10127..13015)
                     /locus_tag="OQS60_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2388169.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13541..14515
                     /gene="hemB"
                     /locus_tag="OQS60_002442"
     CDS             13541..14515
                     /gene="hemB"
                     /locus_tag="OQS60_002442"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS2388170.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14621..15472)
                     /gene="tauD"
                     /locus_tag="OQS60_002443"
     CDS             complement(14621..15472)
                     /gene="tauD"
                     /locus_tag="OQS60_002443"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS2388171.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15469..16296)
                     /gene="tauC"
                     /locus_tag="OQS60_002444"
     CDS             complement(15469..16296)
                     /gene="tauC"
                     /locus_tag="OQS60_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS2388172.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16293..17060)
                     /gene="tauB"
                     /locus_tag="OQS60_002445"
     CDS             complement(16293..17060)
                     /gene="tauB"
                     /locus_tag="OQS60_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS2388173.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17073..18035)
                     /gene="tauA"
                     /locus_tag="OQS60_002446"
     CDS             complement(17073..18035)
                     /gene="tauA"
                     /locus_tag="OQS60_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2388174.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4526 a         4722 c         4389 g         4521 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt
     3301 atttaaataa ttacccacac aaaatatagg cttttaatta tcaaccttta ttataaataa
     3361 aagttatgaa atatattctc ttcgttaact tataattaag agaaaaaaac tcaccgcatt
     3421 gccatttaag ccaggaataa attagcttcc cgttcatcat ttttgtgatc tcgcgcacat
     3481 ctttgagata acgcactttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca
     3541 acaatctttc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt
     3601 tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa
     3661 tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa
     3721 agtcgcttcg acgttgtgct gctgggcatt gataaacaag ggcaatggca cgtcagcgat
     3781 gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg cccttcggcg
     3841 accagccttg cgcaggtgcc aggtaaacat gagcatcagc ttatcgacgc gcaaaacggt
     3901 cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct gggcgaagat
     3961 ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg
     4021 gcttcagcag cctgtatgga taaagatgtc accaaacgtc tgctacgcga tgccgggctg
     4081 aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa
     4141 gtggagtcta aactggggtt accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt
     4201 ggtgtcagca aagtaaccag tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag
     4261 ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt
     4321 ctgggcaacg acaatccgca agccagcacc tgtggcgaga tcgtactcac cagcgatttc
     4381 tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggtagt tccggcagcc
     4441 attgcaccag agatcaacga taagatccgg gcgattgccg ttcaggctta tcagacgttg
     4501 ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga agtggtgatc
     4561 aacgagatca acacactgcc tggcttcacc aacatcagta tgtatccgaa gctgtggcaa
     4621 gccagcggtc tgggttacac cgatctgatc acacgtttga ttgagctggc gctgaagcgt
     4681 cacgctgcgg ataacgcatt gaaagccaca atgtaatatt ttaacgcccg ataatattct
     4741 tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa
     4801 ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc ttcaaatccc
     4861 agtaacccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc atagtagtgc
     4921 cagtcacggc ggatttttac aggcagattc atggcagctc cgttaacgaa aaacaacatc
     4981 cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca ttttcgtaat tgttaataaa
     5041 tttaacttaa ggttgccatt ttgtaactca caacgaagat tcacgcttcg ggaatattcc
     5101 caacatcaat ttaccatgat tctgatattg acaatctaaa tgacctgtca gacttgccat
     5161 aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt tatggctgat
     5221 ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc aacgcctggc
     5281 aacattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca attgctgaac
     5341 ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc aggtccagga aacaggtcgc
     5401 ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc gtttttagta
     5461 ggctgcctca tttttgcagt ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa
     5521 gatttacgtt gccagatacg gcctaaaacg ccttatctgg catgacattc ctgtttgatc
     5581 actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca
     5641 agttgcgggt gattttcggg ctttccagtt gaatgccagg caacatttcg cggggtagtg
     5701 atttacccgt tttcgcttcg gcaagttgat aaactttctt gtacagcgcc gtctcctcaa
     5761 aagagaagct gtcacctttc tctaactgac ggcgaatttc gctgtcgctc atccccagtt
     5821 ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg ttctttactg tcgtaacgaa
     5881 ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt actgactgcg ttctgaaacg
     5941 cggcattacg gctggcgtac cagccagcgt taaaatcagc aaagcggtat atcggtgcgc
     6001 tatagctggc gggataattc agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac
     6061 tgaagacttc ctgacgaact gtaccgtcca ttttccacgg ataccctttg gtatgctgtt
     6121 cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg caccggatta agcgaaccaa
     6181 acagcgtctg ccccattggt accatgctga tcagatcgtc aaaaatcgcg ctcaattgct
     6241 tttctgtacg caccgaatct aaccgttcgc tataactttt gccgtttggc gatttgattt
     6301 ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct
     6361 cttgccaggc gatcttgctt aagcccggaa cggccggatc tgcctgataa tttgactcct
     6421 gctgcgccac cgccagcacc gagcagacgt tttccagcgt cggtgccagc ccctgacttt
     6481 caaaggtgat ggcgagatct ttcgcccagg catcgcggtc tttcacgctc gcaggcatct
     6541 tctgccgcac gacgctcgct acatctatcg ctttttcgcc ctttttcaac ggttgcggag
     6601 cctgactact acaccccgct aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat
     6661 tgacgcgcga cattacttct cctttttagc tcaaggtatg ggttacttcc tgaattttgt
     6721 caccatccag ctcatgttca aagctgcgca gacgtttgta gatagacatc aactcaacca
     6781 gtgtggtcca tgagttaatc aggtactgga aagcaccgcg aacctgacca aaaacgttgg
     6841 taatctgcgt catcaggccg agcgtaatcg taccggcaac aattgacgga aacagcaaga
     6901 acaaaccgaa aacgttatcg acctgcaaat agagaatgcg ggcgatgttg aaatacatat
     6961 agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct aaacagctcg cgtaccgtag
     7021 gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag ctctttacgg taggcggcct
     7081 ctactcgctg gtttttaaac tccagccccg gcagtttgat ccctaccact gccagccatc
     7141 cggtccccat cagcgaccag acgatagcgg caatcaccag accatacgga atgtgcccga
     7201 taatcggcag ctccggcaca tgcgcggaga gcgttaccag caccggcagg aaggcgatca
     7261 acgtcatgat ggcgttgata aaactgacgc ccatattctc cagcgttgaa gcaaaacgca
     7321 tggtgtcttc ctgcacacgc tgtgcggcac cttcgatatg acgcagttgt tgccagttcg
     7381 ccatgtaata ttcgttcatc gccgtacgcc agcggaacac atagtgactg acaaagaagt
     7441 tgttcagtac actgatcacc accgcgatca gcgcaatccc cagaaagacg cccacttcgc
     7501 ggtaaaattg ttcgatagtg actttatgcg gcgaacttag cgcggtttga atcagatcat
     7561 agaacggtgc ataccaggcg ttgacggcga ccccgacttc caccaaaaac caggtgacga
     7621 agatgatcag tgcagtaccg agtattgacc agtattgcca acggtgcggg ctgtagataa
     7681 accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat
     7741 ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc ggaagcgccg gtgatacgcg
     7801 ccacccagtc tcccccaccg gcttgccaga agataacggc aatcaatgcc caaacaaagg
     7861 ccgagagaaa aaacgctccc ggctttggga aaaaagactt aaacatatgc actcctgcta
     7921 acttcttatc gttttaacaa ctgctgtgta ttaaccgcat gaccactaca aagcgtcagc
     7981 caaatgaaac cgcccattgt gacccatagc aaggcgctta gttcgccagc ctgaccataa
     8041 gaattgtttc gacggatttc acaaaccaaa gattgtttac ataagcggat taacacgcgc
     8101 aggatcacaa atgcaggcat tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt
     8161 ttttaacact attattgcac taattcgccc tttgcaatcc atcaatgagt agtataaata
     8221 cgctcagtta ccttcattca atctatggac accaccgttg aaacgtagtc tgcttttttc
     8281 tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg gcgcagccga tcactgaacc
     8341 ggagtttgcc tctgatattg tcgatcgtta tgccgatcat attttttacg gcagcggtgc
     8401 aacggggatg gcgctggttg ttatcgacgg taaccagcgc gtctttcgta gttatggcga
     8461 aacgcgacct ggtaataacg ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct
     8521 caccaaacta atgaccagtg aaatgctggt gaaattgctc gaccagggga ccgtaaagtt
     8581 aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc gtgccgacct acaatgggac
     8641 gcccattacc ctagtgaatc tggcaaccca taccagcgcc ctgccccgtg aacagcccgg
     8701 tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct
     8761 ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt
     8821 cgacctgctg gcagatgcac tggcgaatgc gtccggcaag ccttataccc agttgtttga
     8881 agagcagatt acccgtccat tggggatgaa agacaccacc tacaccccct caccggatca
     8941 gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc
     9001 aattggcagc ggcggcgtct attccacgcc tggcgatatg atgcgctgga tgcagcagta
     9061 tttgtcatct gatttttatc agcgcagtaa ccaggcagat cgcatgcaga cgttgattta
     9121 tcagcgcgcg cagtttacga aagtgattgg catggatgtc cccggcaaag cggatgcgct
     9181 tggtttaggc tgggtatata tggccccgaa agagggtcgt ccggggatta ttcagaagac
     9241 aggcggtggt ggcggtttca ttacctatat ggcgatgatc ccgcagaaga atattggcgc
     9301 gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa aatatgagcg atggcatcaa
     9361 tgatctggtg accgagctaa gcgggaataa accgttggtt atccccgcat cctgattaat
     9421 actctgaagg caaacggttg atagcgacca gtttgccttt attcatttcg atatagccgc
     9481 ctttacgcaa agcagcgaga acttctgcga cgacagaacg cgatatgcgt gtacgttgat
     9541 ggatataatt catcacacca atacgtgatc gcaattcttc attccagtca atcattgata
     9601 ataatgttgc gcggatttgt tcgtaggaat tatgcccaat gagctgcacg tcgcgtaatt
     9661 ccagaattct attttgccaa gctaaccagt aaaaagcgtc tcgccagagt tgattttgtt
     9721 caataagcgt aatggtttgt ttggctggta gatgatatcc cgtacaattt ccttctgata
     9781 ttaatttgta tggtatatcg tttttcatta aaccatcagc cagccccata atataagggg
     9841 cctgggtaat accgataagt acgttttctt ctctacgcag agagataact ccctctaaaa
     9901 taacgaaagt atcttcgtta ttgacatcac tggaaaatat aacttgcttt tcattattaa
     9961 actcgaagcg cgtaccgtat ctggacaaac atttatcgag cttaccaaat tcctgaagag
    10021 gtttaactac agataacatt tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg
    10081 gcattattta gattaaatta ccagtatttc ttcggagtga agaatattac caggtatatt
    10141 taacacccac gttcgcggac cagtcttgat ctacgtcacc accaccgagg tagttagcat
    10201 cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca acacgtaccg
    10261 cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg ttgttagagt
    10321 cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctgatcttcg ctgtaggtga
    10381 aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac tgaccgtcaa
    10441 ctttcatgtc gttgctcagc tggtagtcat caccagactg gaacagacca gaaacgctgc
    10501 cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc gtaaccggct ttcaagccga
    10561 agccccaagc gtcggagttg gtgctaccgt caacgtaagt accgttgctc atggttgcag
    10621 acaggtcgtt gttgaagtga gagtagctca agctaccatc aacaaagacg ttgttcgcga
    10681 agtgagcaga agagtagatg taggcagtct ggctgtcttg atctacctga ccagaacggt
    10741 cattcatgtc acctttagcg aagcctgcag ccgcaccgac gatccactta gcgttgttac
    10801 cgtcaacttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca tagttgatgg
    10861 tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg ccgttatcag
    10921 ccaggccatg acgagagttg gtcagacgag taccaacggt gtcttgttcc aggttccaga
    10981 tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc agctccatct
    11041 gttgcagaac aacggtgtta ccgcgctgtt cagcctgata ggtgtatgca cccaggtcag
    11101 ctttgttagc agcagagaag gtcgcgttgg ttttggtatc atcgatatag atgagctgct
    11161 tacctgcgta atcagcaatc gcaccagcgc cagtggagtt gtcgatacga actttgtagt
    11221 taccggttgc agctacaacg ttgtctgcag agctgttgtc aacttcgtcg ccattgttaa
    11281 tgtcgttgtt accattaacg gtcaagtgac catcagagtt catcgcaata acaccataac
    11341 cgtagttagc tttgctggtg tcgttggtac gatcgttgac gaggtctgca ttcaggatgt
    11401 aatcattact atgaatgtca aacacacctg cgtaaacatt accgttgtta tctttaacgt
    11461 tgccactggt cagttccaga gtatcagttg caaatacgct ggagatgtca ttcatgttgg
    11521 tgtttacatc cagcacacca ccgttagtaa cggtaatggt attggcatac agcggtgcat
    11581 atgcccaagc tgaagcagta tcttcaccga agttaacggt tgagaaggta tcaatggtca
    11641 ggtgatcggt gttaacttca ccgttaactt ccattgaaga accaccgttc agagtgatgg
    11701 tgtcagaagt cagagaagaa tctgcactca cgttaacctg agaaccgctg ttgatcgcca
    11761 gggtatcgat cagggaagtt ttcgtggttt cccaatcaga accgttgttc agaacaacat
    11821 cgaatacgcc gctctggaat gcgttagcgt tatcaacacg attccagcca tcttcagagt
    11881 aagaagcgta cgggttcagg ctgtttggtg caacgtcgta gaaggcttcg tcagcaacaa
    11941 caacgtcata atcaatattc gctgaaccaa cccatttggt gttgttcaga gtcaggttca
    12001 ggtgatcgac gttctgatcg tcagttgccc aaccgccatg agtgtaagca gtggtagagt
    12061 tgtcaatcag gtgacccgtc gcgtcaaagt tagcgttcca ggtactgtca aatacaacgt
    12121 caccaagcaa ctgagagttg ttgaacgtaa cgttgttgac catacgataa tcagaagtgt
    12181 catcatcgat gaaggctaac gcaacatcat cagcaggaga tgcgccattg ccgtagtcac
    12241 ttggttcaac agaattgccg aaatgaccac gatctgattg ttcgtcttca gtccatgaac
    12301 cagaagttac agtagagtca ttaacaacga tgttgttgtc ccactgatga ccttcggtat
    12361 tgtaggtcgc attaccgagg tcgtgttgaa ccaaagtaat acctgcaaca tgggagttgt
    12421 tgctgatgtt gatgttactt tcaacatcca gagtcacagc ggtaccaaga ccagcggtta
    12481 ccaaacgatc ataatctgtc ttagatgttt taccgtctgc atccagataa gtatcggtga
    12541 agtagaaggc gtcaaaatca tcgtcaatag tagaattagc gatgttcaga gtgaagatgt
    12601 cgccatcaac ccagtcgtgg ccagtataat cgctaccgtt ggtccagacg tagtcgccat
    12661 aaccaacctg gtaagatgta accataccat gaacaattga gttggagatg ttcagtgtat
    12721 taccggtatc tgtgccataa tcgtggttca gataataagt agaaattaca ccatttacag
    12781 tgtcgccgct gaaagtggta tcaaagtaac catcagctgc gtggtcatat acataaccac
    12841 cgtaagtcag ataatcgtga tcaattttag tgctgtcacc ccaattgtag atggtggctg
    12901 cgtcatcgtg atacccgaag gtgttataag tagctgcatt agcctgagag gtgatagcca
    12961 gagtgcaagc caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc
    13021 atccctcctc agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt
    13081 atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg gttcaattat tctttcccct
    13141 aagtccgaat gtggacatat ataatttatg gagaaaaaca cgcatcaaat tgataaaagg
    13201 acgatatttc aacatgttgc agataatgtt atttttgata catacgcgtt ataaatatca
    13261 aaaacacaaa ccagataaat atcttatcaa tatacaacaa agtttgaatt attcaattta
    13321 tagcagcaca accaaacgcc aaaaaaaata ataaccaaga gctataaata aagattaaaa
    13381 atattttcag tgaatatgaa attattgcta tttctattac aaagatgtcc tggtctctct
    13441 ttcattctct tcttgtcaac atcacgacaa ctttcgtaaa acactcctgc cccgcttcag
    13501 gtatactgtg caccttaatt ccacaaacat caggcagacc atgacagact taatccaacg
    13561 ccctcgtcgc ctacgcaaat ctcctgcgct gcgcgctatg tttgaagaga caacacttag
    13621 ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa attgacgact acaaagccgt
    13681 tgaagccatg ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg aaattgaacg
    13741 catcgccaac gccggtatcc gttccgtaat gacttttggc atctctcacc ataccgatga
    13801 aaccggcagc gacgcctggc gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa
    13861 gcagactgtg ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat acacttctca
    13921 cggtcactgc ggtgtgctgt gcgagcatgg cgtcgacaac gacgcgactc tggaaaattt
    13981 aggcaagcaa gccgtggttg cagctgctgc aggcgcagac ttcatcgccc cttccgccgc
    14041 gatggacggc caggtacagg cgattcgtca ggcgctggac gctgcaggct ttaaagatac
    14101 ggcgattatg tcgtattcga ccaagttcgc ctcttccttt tatggcccgt tccgtgaagc
    14161 tgccggaagc gcattaaaag gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg
    14221 cgaggcgatt cgtgaatcac tgctggatga agcccagggc gcagactgcc tgatggttaa
    14281 acctgctgga gcgtacctcg acatcgtgcg tgagctgcgt gaacgtactg aattgccgat
    14341 tggcgcgtat caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc tggcgggtgc
    14401 tatagatgaa gagaaagtcg tgctcgaaag cttaggttca attaagcgtg cgggtgcgga
    14461 tctgattttc agctactttg cgctggattt ggctgagaag aagattctgc gttaattttt
    14521 ttcctctcag aaagggggag ttaacctgtt gaaagtacgg tgaacgcccc ctcaccctaa
    14581 ccctctcccc aagggggcga gggaaccgtc cgcactcgta tcaccccgcc cgataaaacg
    14641 gtttatcccc aaggatcgtc gcccgatgca taatccgccg ctgtggcagg taatcggcat
    14701 tggcatagtg ctgagtcacg cggttatccc aaatcgcaat atcattcggt tgccagcgcc
    14761 aacgcacctg aaactccggt ttagtgatat gggcgaataa aaagcctaac aaggcttcgc
    14821 tctctttctc gctgacatcg acaattcgcg tagtaaagcc ttcattcaca aacagcgcct
    14881 gtttaccgct caccggatgc gttcgcacca ccggatgtag caacggagga tttttcgcga
    14941 ccgcctcgcg ccagcgttga tgttcctcct cggttttgcg gtatttgtat tccgggaacg
    15001 atttacggaa atcatgctcc gcacgcagcc cgctcagcag ctggcggaag ggaacagaga
    15061 gcgcctcata ggccgcaata ccgctggtcc agagcgtatc accgccggtc gaaggtaact
    15121 ctttagctgc cagaatcgcc cctgcgggtg gcgtttcaat aaatgtcaca tcggtatgcc
    15181 agttgtcgtt atccggcgga ttatcgttat gggtatccag cacgatgatc tcgtcaaccc
    15241 cttcggcatg agggtaaaca gggtgaatat gcagttcgcc aaaacgctgg gccagcgcgc
    15301 gttgctgctg cggcgtaata gcttgatcgc gcagaaatac cacctgatag cgcagcaccg
    15361 catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgtcaggtcg gcacccgaaa
    15421 tttgtgcgcc gatatacggc cccagcgggg taatactcag acgttcactc attgtacttc
    15481 tccatgccag ggcgtcagac ggcgctgtaa cgcgcgcaga cccagttcta aaagaaaggc
    15541 gataatcgca atcaccgcga tccccgccag taccacgtca gtcgcgagaa attcacccgc
    15601 tgactgaacc ataaatccta aaccgcgtgt cgcggcaatc agctccgccg ctaccagcgt
    15661 agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca gcgcgccggg
    15721 caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg cggcacgaat
    15781 gcgaacctgc tgcgcgcttt tcacccccgc cagcaccgac atcgccaccg gtgcaaaaat
    15841 cgctaaatag atcaataaga tcttcgaggt ttcaccaata ccaaaccaga tcaccatcag
    15901 cggcaaataa gccagcggcg gcaccggacg ataaagttcg attaccggat ccagaatgcc
    15961 gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca ccaccgccgc
    16021 gagtagcgcc agcacaatgc gcgtcaggct ggctgccaga tgctgccaca gcgtggcgtc
    16081 cataaatccc tgcggtccgg caatggtgag cagttttgcc agtacttgtt gcggcggcgg
    16141 caaaaacagc gggctaatca gttgcagcgc cgccaccgtc caccatacag tgagtaaaac
    16201 cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc atttcagccg
    16261 ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg ttgctcaaat
    16321 acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt gatgctgcgg
    16381 ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc cagcacacgg
    16441 ccagggccgg atgaaagcag aaccagttca gtcgccataa acaccgcttc ttctatatcg
    16501 tgggtaatca acagcacctg cttgcccgtc tcctgccaga gtttcagcag cagagtttgc
    16561 atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa taacagctgg
    16621 ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac gttgaccacc ggaaagctgc
    16681 cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg gtgcgcgatt
    16741 tccagtcgct gcattttctc tatacctgcc aattgcaggc cgaacgccac gttgtcctgt
    16801 acattgcgcc acggtagtag cccttcattc tgaaaaacca ccccgcgctc tgctcccggt
    16861 ccctcaatac gcttacccgc cagttgaatg ctgccatgct gataaggcac aaaaccggca
    16921 atcagattca gcagggtggt tttaccgcag ccggacggcc ccagcaccac cagtagctcg
    16981 ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata atcggcatag
    17041 agatgagaga tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga ggtaacgtac
    17101 tggctgtaat cattcgctac agccgggacc ttgccctgct ctttcaaaaa ctgcgcggtg
    17161 tcgatgattg ctttgttcac cggtccggtc agttctgccg tttgttgctg cggcgtcaga
    17221 taggtattcc ccttcaccag ccccggaacg tcaccttccg gcacgccgct taaacgcgcc
    17281 agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat gtacggttgc
    17341 tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt ctcggcaaaa
    17401 tctttgcgca ccacccagac gtccagcgtt ggcgcacccc actgcccgac ctgttcagaa
    17461 tcggtcaaca ccttgccgtc tttttccagg gcgttaaccg ccggtgccca gacataagca
    17521 ccatcaatat caccccgctg ccatgcggcg ataatcgcgg gcggctgcag gttcacaatc
    17581 tccacttgcc ctggtttaat cccccagtgt ttcagcgccg ccagcaggct gtagtgggtg
    17641 gtggagataa acggtacggc aatgcgtttg ccaatcaaat cttcgggttt gctgatagtt
    17701 ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa gacttcaatc
    17761 ggcacctgtt ggctggctgc aaccgctaat gggctggaac cgaggttgcc gatttgcacg
    17821 tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg ccagtccacg
    17881 ctcgccccgc tctctttagc aaaggtgttg tcggcctgag ccactttcgc cggttcggct
    17941 gaggtttgat acgccacggt gacgttcacc gcctgtgcct gaaaagcgat gaatgccagt
    18001 gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc tgtcttgtta
    18061 tgggagcagt attcaggaat aaaaacattc attaaaagaa ttagtcgtta tcgcacagat
    18121 gattttattc ttagcaaaaa aacggcaaag gaaagatt
//
LOCUS       DAJVYM010000078        17959 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVYM010000078 DAJVYM010000000
VERSION     DAJVYM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.078"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="OQS60_002447"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="OQS60_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS2388175.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="OQS60_002448"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="OQS60_002448"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS2388176.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="OQS60_002449"
     CDS             4390..4641
                     /locus_tag="OQS60_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS2388177.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="OQS60_002450"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="OQS60_002450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS2388178.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="OQS60_002451"
     CDS             5946..6704
                     /locus_tag="OQS60_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS2388179.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="OQS60_002452"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="OQS60_002452"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS2388180.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="OQS60_002453"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="OQS60_002453"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS2388181.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="OQS60_002454"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="OQS60_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="OQS60_002455"
     CDS             complement(9558..10178)
                     /locus_tag="OQS60_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS2388182.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="OQS60_002456"
     CDS             complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="OQS60_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS2388183.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="OQS60_002457"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="OQS60_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS2388184.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="OQS60_002458"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="OQS60_002458"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS2388185.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="OQS60_002459"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="OQS60_002459"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS2388186.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="OQS60_002460"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="OQS60_002460"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS2388187.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="OQS60_002461"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="OQS60_002461"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS2388188.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="OQS60_002462"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="OQS60_002462"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS2388189.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4750 c         4488 g         4406 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tccccctgcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgct
//
LOCUS       DAJVYM010000079        17891 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVYM010000079 DAJVYM010000000
VERSION     DAJVYM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.079"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            16..1092
                     /locus_tag="OQS60_002463"
     CDS             16..1092
                     /locus_tag="OQS60_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCS2388190.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1485..1826)
                     /locus_tag="OQS60_002464"
     CDS             complement(1485..1826)
                     /locus_tag="OQS60_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS2388191.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1839..2711)
                     /gene="copD"
                     /locus_tag="OQS60_002465"
     CDS             complement(1839..2711)
                     /gene="copD"
                     /locus_tag="OQS60_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCS2388192.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2715..3089)
                     /gene="yobA"
                     /locus_tag="OQS60_002466"
     CDS             complement(2715..3089)
                     /gene="yobA"
                     /locus_tag="OQS60_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS2388193.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3228..3458
                     /gene="holE"
                     /locus_tag="OQS60_002467"
     CDS             3228..3458
                     /gene="holE"
                     /locus_tag="OQS60_002467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS2388194.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3560..4216
                     /locus_tag="OQS60_002468"
     CDS             3560..4216
                     /locus_tag="OQS60_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS2388195.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4240..4902
                     /gene="exoX"
                     /locus_tag="OQS60_002469"
     CDS             4240..4902
                     /gene="exoX"
                     /locus_tag="OQS60_002469"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS2388196.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4899..6959)
                     /gene="ptrB"
                     /locus_tag="OQS60_002470"
     CDS             complement(4899..6959)
                     /gene="ptrB"
                     /locus_tag="OQS60_002470"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS2388197.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7168..7827)
                     /locus_tag="OQS60_002471"
     CDS             complement(7168..7827)
                     /locus_tag="OQS60_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS2388198.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8154..8510)
                     /locus_tag="OQS60_002472"
     CDS             complement(8154..8510)
                     /locus_tag="OQS60_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS2388199.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8577..8867)
                     /gene="yebG"
                     /locus_tag="OQS60_002473"
     CDS             complement(8577..8867)
                     /gene="yebG"
                     /locus_tag="OQS60_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCS2388200.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9001..10179
                     /gene="purT"
                     /locus_tag="OQS60_002474"
     CDS             9001..10179
                     /gene="purT"
                     /locus_tag="OQS60_002474"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS2388201.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10235..10876)
                     /gene="kdgA"
                     /locus_tag="OQS60_002475"
                     /gene_synonym="eda"
     CDS             complement(10235..10876)
                     /gene="kdgA"
                     /locus_tag="OQS60_002475"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS2388202.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10913..12724)
                     /gene="edd"
                     /locus_tag="OQS60_002476"
     CDS             complement(10913..12724)
                     /gene="edd"
                     /locus_tag="OQS60_002476"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS2388203.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12959..14434)
                     /gene="zwf"
                     /locus_tag="OQS60_002477"
     CDS             complement(12959..14434)
                     /gene="zwf"
                     /locus_tag="OQS60_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS2388204.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14772..15641
                     /gene="hexR"
                     /locus_tag="OQS60_002478"
                     /gene_synonym="yebK"
     CDS             14772..15641
                     /gene="hexR"
                     /locus_tag="OQS60_002478"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS2388205.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15769..17211
                     /gene="pyk"
                     /locus_tag="OQS60_002479"
     CDS             15769..17211
                     /gene="pyk"
                     /locus_tag="OQS60_002479"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS2388206.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17271..17747)
                     /locus_tag="OQS60_002480"
     CDS             complement(17271..17747)
                     /locus_tag="OQS60_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCS2388207.1"
                     /translation="MDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDPDSAINI
                     TKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYITINDSIK
                     TWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         4557 a         4507 c         4342 g         4485 t
ORIGIN      
        1 tggagaagtt aaaacatgca gcggaaaaaa tacgatccca atttgcctag aaacttaaca
       61 tatcgaagga gagacaaagc atattactgg cgcaaccctc tgacgaaaga agaatttaca
      121 ctaggtaaaa tttcaagaag agatgcagta acgcaggcaa ttgaagcaaa tcattatata
      181 tacaaaaact actctcccgc tgccttaatt gaaaaactta aagggttcga ctcatttact
      241 atggcagact ggattgaacg ttacaaaacg attcttataa ggagaaaagt gtccagaaat
      301 acttataaaa ttcgggcaaa tcaactggag acaataaaag aaaaattggg agagatttta
      361 ctgacagaaa taaccactcg ccatattgcc gagtttcttg atttgtggat tgaaggaggg
      421 aaaaacacaa tggcaggatc aatgcgttct gtgttgtctg atatgtttcg cgaggccatt
      481 gttgaaggac gtatatctca aaatccagtc acgccaacaa gagcaccgaa aatagtagtt
      541 acaagagaac gactgaaact aaagatatac aactgcatca gggaggcagc agatcaactt
      601 ccggcatggt tcccattagc tatggattta gcccttgtaa caggacaacg tcgcgaagac
      661 ataacgaata tgcggttcag tgatatttat gatgatcgtc tccacgtcag gcaaattaag
      721 acaggaatga tgattgctat ccccctgtca ctcagccttc ctgtcgctgg tctacggctt
      781 ggtacagtag ttgaacgatg ccgcctggta agccggggag attatctaat cagtgccggg
      841 attagaaaaa acagccctga cggcagcatt cacccggatg gcctgacaaa aaaatttgtc
      901 gcagccagaa aattcacagg tatccagttc agtgaaaacc caccaacttt tcacgagatc
      961 agaagcctgg ctggacgatt gtacaaagaa acatgtggag aagaatttgc tcagcgtcta
     1021 cttggccaca catcggagaa gacaacaaaa atgtatcttg atgagagaga aaaaacgtac
     1081 ttactgctct gattttaacg taaatggatt gttaaatgta ttttggttgt gatataacca
     1141 aaaagaccgg aatacagaaa ttcgagtaaa tttcggggaa tttcggggga gacatttgca
     1201 actaattgat ttcaaatgca attaaaaaaa gaccgaatac gattcctgta ttcggtccag
     1261 ggaaatggct cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg
     1321 ccttgccctt taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca
     1381 ataaaaagcg cggtggtcat cagcttaaat gttaaaaacc gcccgttctg gtgaaagaac
     1441 tgaggcggtt tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca
     1501 gctcgttcta taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc
     1561 tgaatcaccg aaatgggttg ggcattggtc ttcccactgg caacttcctt ttgtgcgata
     1621 tcgtttaaag gatactgcac gagggtactc ggattaataa catacaaagc attacccggt
     1681 cggcaagtca gcatcacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa
     1741 cgactgacgg taatcacctg cggtgcagcc agcgccgctg cagaactggt gagtaacaga
     1801 aacgccagaa tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca
     1861 agcaggctga tgatcaccag cactaccgca cctatcgccc attcgagttt agtcatccac
     1921 acaaaccagg gtgccgcacg atcttcctcc tgcctcatgc gcggtacaag aacataacga
     1981 ttagccagcg caatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg
     2041 ccccagtaag tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg
     2101 cttgccagta cgccgatcac cgcaaaatgc ccgcaccatg aaaaacacat cagcgcctga
     2161 atagcctggt gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat
     2221 cccccaaacc acgccgccgc acaaatcagg tgaatcgcgt gattagtctg atggattttc
     2281 gccgttaccc cttcattcag tgtcgcatgc cccattcccg ccagcagaat aaattgcgcg
     2341 gtagtgagca taaacagcaa acgtggcata ttccgcggtt gcataagggc gacgatcaac
     2401 gttacgaggg cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca
     2461 gcctgccaaa tgtttggcga aaatacatct gtccatcccg ttcccatcaa cccgccctga
     2521 acggcgagca ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc
     2581 agaaaacgct tcgccagcaa gcgacgaatc gtcaaggggg ccagccaggc accgtacatt
     2641 gcgaagccaa aaaccagcat cagcgaggtg aaatggataa atcgcagcgc gatccaggta
     2701 aacgccagca tgatttattt cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc
     2761 accgaaacaa catgccagtc aacggtgtag gttccgggtt tcagcgaatc ggccagtgga
     2821 acaatcagtt gcttttggtc ctgctcattt cgcttcgccg gtaatgtttt aatattttcg
     2881 ttttttggcc ccgtgatttt tgcaccactg aatcctgttt ccacaccttc cgagaagttt
     2941 aaagtgattg cctgcggtgc agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt
     3001 aaatgcgcat gtgcccagac gaaaggtgtc accaacgaag tggtcaggat tgccagcgcg
     3061 tagcgaaggg agcgtgcagt tgaagccata ttatctattc ctttttgtaa taactttttt
     3121 acagagcata accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcct
     3181 ggctcactct tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc
     3241 tggctaaact ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggccgggg
     3301 tggcatttaa agaacgctac aatatgccgg tgatcgctga agcggttgaa cgtgaacagc
     3361 ctgaacattt gcgcagctgg tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca
     3421 atctgtcacg tttaccttac gagcccaaac ttaaataaaa cttatacagg gttacacttt
     3481 cttacataac gcctgctaaa ttatgagtat tttctaaacc gcacccataa tttgcagtca
     3541 ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg
     3601 taaaacctca ctcaccgaac aagtagctca tcatctggag tttgtgaggg ccgccgccag
     3661 gcaacaatgt cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg
     3721 acgtgccctt cctgccccac ccgatctctc actgttagac ccgctttgtt atgccgcaac
     3781 gacctggcga atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg
     3841 tggaattgcg gtgttcgcac cgtggcgaaa aacgcctggg atctaccatc agagtcatgg
     3901 tgcatgtctg ggccgtcgtt ccagaaccat cacggtggtc gatgaacaac cggaaggtat
     3961 ggatatggac ccgacctgtt cactgtttac gacagggcaa tgtctgggag aacccgacct
     4021 gctggcgtcg gcccgtcgct tacaattttt ttcacatcag tactcgattg ctgtgctgat
     4081 ggcaaatgcc cgtggtaaca gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc
     4141 cgatcgtggt tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat
     4201 cattccatta cgctaggctt tttcggcctg gagtatgcca tgttgcgcat tatcgataca
     4261 gaaacctgcg gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga
     4321 aaaatcgtca accccatgag ccacctggtg cgccccgatc gtcctattag tccacaagcg
     4381 atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg
     4441 atcccacact attacggcag tgaatggtat gtcgcgcaca acgccagctt tgaccgccgc
     4501 gtactgcctg agatgcccgg tgagtggatt tgcaccatga aactggcccg tcgcttgtgg
     4561 ccaggaatca agtacagcaa tatggcgtta tataaaacac ggaagctcaa tgtacagacg
     4621 ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt
     4681 atcgatatta tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt
     4741 ccgtcgttga tgacgacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt
     4801 gccgaacgcg atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg
     4861 gagctgcgtt taacactgaa acattatctg gaaaatactt aatccgcagg cgtagcgggt
     4921 aatgttccct gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat
     4981 ttaaagcggc cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga
     5041 tggttatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc
     5101 acctgagaat cgtgtaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg
     5161 ttgtcatacg ggctgtagct tttcatgtac tcgtaatatt gcggatcctg cgggttaccc
     5221 cactcttcaa actcaccagt ggtaagagga attgattcat caagcatcgt tgttacaaca
     5281 tcaacaaacg gtacctgggc gataacgccg tggaataatt ccgggcgttg attaattgca
     5341 acgcccatca acatgccccc cgcactcccg cccatcgcat aacaaagcga aggagagcca
     5401 tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc
     5461 ttcagaaatt ttccgtcttc gtaccattgt tgccccagct caccaccgcc gcgaacatgg
     5521 acaatggcgt agacaaagcc acgatctaac aaactcaagc ggctaaaact gaaatcggca
     5581 tcaatacttg cgccgtaaga accatagcca tacaccagca acgggttgtg tcctttgcga
     5641 aaatgtttgc gatggtagac caacgaaacc ggaacttcga cgccatcacg ggcgactatc
     5701 cacaggtgtt cactgcggta attcgccgca tcaaaaccag gaacttccgt ttgttttaat
     5761 acacgacgct catcggtatc catatccagt tcaaacaaag tgtctggtgt ggtcatggaa
     5821 gaataaccat aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca
     5881 taggctggat catcaaaggc aataccaatg acttcccggg tcttgcggtt aatttggcgc
     5941 aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac
     6001 ccttccagca tgatgttttc gcatggcgga attaactctt cccactgttg ctcatcacgc
     6061 atacgggtac ggtataagcc aaagtttttg ccgtggcggt tggaacgcag ataaaaacga
     6121 tgctggtagt gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc
     6181 tcggcatcgg ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaaa
     6241 tgaatgacta catagtgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct
     6301 ttttcttcgt agatcagttt atcttgcgat gctggcgtac cgatggcgtg acgccagacc
     6361 tgataaggca gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggagtcattt
     6421 gcccagacaa agctgggttc aacgttatcc agcagttccg ggtaccagtt accagtttcc
     6481 agattacgaa aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagcgccata
     6541 atggtgttat cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagctgcg
     6601 cgcttattgg catcgagcaa tatttcccac tcatcccact cttcactgaa tgccgattga
     6661 cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt
     6721 ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg
     6781 cgatcctgca aggcttgttg tgaggccatc acccgatgac cgtaactatt ttcttgttgc
     6841 aggtagtcca ggacttctgg ctgagaacgc gtatcatccc gcagccagta gtaattatcg
     6901 atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg
     6961 ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag
     7021 cgaaacaggg gagtcattgc ttagatgatg acaggtaatg gcacggatat cgaatgttat
     7081 gcagacagag aaactcagcc tgttcaaatg gctgtgcggt tctggatagc ccgaaatagt
     7141 caacttcagg ctatccagag agcggaatta ttccgccaaa gtgcgttttt gctgttcgag
     7201 atcgcgttca atgccgtcac gaacatcctg ggggattttc agcgcgtcac ccagtgcatt
     7261 caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcaactca ggaaatagat
     7321 ttccagcgcc tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg
     7381 ttcgattgcc tgctcaatga gtacacgccc ctgctcttcc acgccggctt cacgcaattg
     7441 ctggtcgatt gccgcacgtt ctttggcatc aatatgacca tcacttttag cggcaaagac
     7501 cagcgcaagg atcaaacgtt ctgtacgctc atccagcggc gtactttgcg cgccaaactg
     7561 cggttcgtcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc
     7621 cgctaccgct ccgccgccaa ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg
     7681 tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgcctg gcactaacag
     7741 tttgcccaat ccctgatccg cagacgagga ggtagaagaa ctgctttgcc caagcaggga
     7801 ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt
     7861 agcatagcgg atgaggatgt cagaaaatgt atatcgagac aaaagatgcg caaataagcg
     7921 gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtatttact gtatatcagg
     7981 tgataagggt attttaggtg aaagtagttt gtcgttttga gtggcattgt ttgatgctga
     8041 ttgcccgatg cgacgctgtt gcgtcttctc aggcctacga ttgtggtgcg ttctggggcg
     8101 gacaaggcac tcgcgccgca tccaccaacg gtcaatcgaa ttatcagaag ccattaacgc
     8161 cgctgatatt ccgccattcc cgctttgcag tccacgctga cctggtaatg aatatcggca
     8221 cttttaccac gcacggttag cggtactgac catttatcat ctttaccctg aatgtcctgc
     8281 aaactgaccc aagccacggg atcggcctga ccgacaattt tttgatcatc tgcccaacgc
     8341 gccacgcgat tttgttgata atcacgtttt acgctcgcgg caattccggc agcatccaga
     8401 ccttcacact ttgggaaagt gaccgacttg ctggtttcat tattggcagc gaaaactgat
     8461 gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc tttttttcat gtttttctcc
     8521 atagcacaat gattcaggag aaagcatggt acaaattgtc aggagcgcaa gttgcttcag
     8581 gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga cgacctgtgg ggacggtaat
     8641 ttgccggttt tcagaatggt gctcagcaca tctttttgtt ctgccagcca tagcgaaagg
     8701 gcttcacgtt gctcgtcttc catttgcact ggagaatttg tcagccaggt gtcaagcaga
     8761 tccgccgtat caagcatttt gtcataagca tcggcttcct ttttgctggt aaacgacatt
     8821 ttctcttcgc cctcacgaat gactacgtat ttaacttcaa ccgccatttg cagcctctca
     8881 taataactgt gattttatac agtatatttc ttttcggttg agaaatcaac atcagcaata
     8941 aagacacacg caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt
     9001 atgacgttat taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc
     9061 ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc
     9121 gtcgatcgct atgccgacgc accagccatg catgtcgcgc atcgctccca tgtcattaat
     9181 atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg
     9241 ccggagatcg aagctattgc caccgatatg ctgatccaac ttgaagagga aggactgaat
     9301 gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg
     9361 gcggcagaag agctgcagct gcccacttcc acttatcgtt ttgccgatag cgaaagcctt
     9421 ttccgcgagg cggttgctga cattggctat ccctgcattg taaaaccggt gatgagctct
     9481 tccggcaagg ggcagacgtt tattcgttct gcagagcaac ttgctcaggc atggaagtac
     9541 gctcagcaag gcggtcgcgc cggagcaggc cgcgtaattg ttgaaggcgt cgttaagttt
     9601 gacttcgaaa ttaccttgct aaccgtcagc gcggtggatg gcgtccattt ctgtgcaccg
     9661 gtaggtcatc gccaggaaga tggcgactac cgtgaatcct ggcaaccaca gcaaatgagc
     9721 ccgcttgccc ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc actgggcggt
     9781 tatggtctgt ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt cagtgaggtc
     9841 tcccctcgtc cacatgatac cgggatggtg acgttaattt ctcaagatct ttcagagttt
     9901 gccctgcatg tacgtgcctt cctcggactt ccggttggcg ggatccgtca gtatggtcct
     9961 gcagcttctg ccgttattct gccacaactg accagtcaga atgtcacgtt tgataatgtg
    10021 cagaatgccg taggcgcaga tttgcagatt cgtttatttg gtaagccgga aattgatggc
    10081 agccgtcgtc tgggtgttgc gctggcgacc gctgagagtg ttgttgacgc cattgaacgc
    10141 gcgaagcacg ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca
    10201 aaaatgcccg atcgtagatc gggcgttttg acttttacag cttagcgcct tctacagctt
    10261 cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc cagcgcatct gccggaacca
    10321 gccaggaacc accgatgcac agcacgcttt tcagcgccag gtagtcacgg tagttagccg
    10381 gagaaatacc acccgtcggg cagaaacgga cctgggagaa cggacccgcg atcgcctgca
    10441 gggctttcac gccgccgtta gcttcagccg ggaagaattt gaactctttc aaaccgtagt
    10501 ccatacccag catcagttcg gaaacagtgc tgatccccgg aattagagga atagtccctt
    10561 cggtagcagc tttcagcagc ggctcggtca gacccgggct aattgcgaac tgtgcacccg
    10621 cttcagtgac ttctgccagc tgctgtggat tcagcaccgt accggcaccc acaatcgctt
    10681 caggcacttc tttggcgata gcacggatag cgtcaactgc acactcggta cgcagagtca
    10741 cttccagaac gcgcacccca ccagcaacca acgcttttgc catcggcact gcgtgttcca
    10801 gtttttttac cacgataacc ggtacaaccg ggccggtggt caggattgat tctgcacttg
    10861 ttttccagtt tttcatcaga gttttctctc gcctgattac aaatttgtcg tcttaaaaag
    10921 tgatacaggt tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg ctgaataatt
    10981 cacgtcctgt ccccacgcgt gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca
    11041 gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt
    11101 ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc ttctggtgtt acgtggatag
    11161 ctgacggcac tttacctgaa gcgccggaga gtcgtccatc ggtaactaac gcaattttga
    11221 aacaccggtc caataataca ccaagtggcg gcatgagttt atgtaattct ggcattccgt
    11281 tcgcttttgg cccctgatga cggacaacaa cgacacaatc gcggtccagc aaacccgctt
    11341 caaaggccgg cataacgtca tgctggcttt caaaaacaac cgctggcgct tcaatcacct
    11401 ggttctcaac cggcacggca gaggttttca taaccgcacg gcccaggtta ccgcttaaca
    11461 cttttgtccc accatgatga gagaaaggtt gttcgaagga agcgatcaca ttgctgtcga
    11521 gtgatttttc cgccccttcc cgccagtcct gttcaccatt attcagccat ggttcaaggg
    11581 tataacgaga cagaccaaag cctgccaccg tattgacatc ttcatgcagc aggcctgctt
    11641 tgagcagttc acgcaccaga accggtacgc cacctgccgc ctggaagtgg ttaatatcgg
    11701 ccggaccgtt cgggtagaga cgtgccatca gcggtacaac atcagaaagg tcagagaagt
    11761 catcccagtt aatctgaata ccggccgcgc gcgccatcgc caccaggtgc atggtgtggt
    11821 tagtggaacc accggtcgcc agcagtgcaa cgataccgtt caccaccact ttctcatcga
    11881 tcatcttacc gatcggcatc cattcattac cattaccggt catgcgtgta acctgacgcg
    11941 cagcggcagc ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg
    12001 gcaactgcat ccccataaat tccaccacca tctggttggt gttggcagta ccgtagaaag
    12061 tacatgttcc cggcgcatgg taagacgcgg cttctgactc cagtaaggcc atgcggtcca
    12121 ctttaccttc ggcataaagc tggcgaatac gcactttttc tttatttggc aaaccgcttg
    12181 ccatcggtcc agacggcaca aacaccgcag gcaaatgacc aagcgacagg gctgccatcg
    12241 tcagacccgg gacaatcttg tcgcacacac cgaggaacag agcaccatca aacatgttat
    12301 gggacagccc caccgccgca gacatcgcta tcacttcgcg gcttagcagc gacaattcca
    12361 ttccatcctg cccctgggtg acaccatcac acatcgccgg aacaccgccc gcaacctgac
    12421 caaccgcatt cgcttcatgc agggctttac gaatgatttc tggatagtgt tcataaggct
    12481 ggtgcgcgga gagcatgtcg ttataggagg tgatgatggc gatattgtta cgcaacatgc
    12541 ttttcaaaga ggctttgtct tctggctggc aggcagcgaa accgtgtgcc aggttaccgc
    12601 atgccaactg cgaacgatga acggtcgacg ttttcgcttg ttctatccgg gcgagataag
    12661 cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt tacgcataac aattgtggat
    12721 tcataaaggc tcctgaaatt gagttgtcag agcaggatga ttcacaacgc gtttcattca
    12781 gaggatttat gagtgaaacg cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag
    12841 tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc
    12901 tacaatctgt gcaagatcat gttaccggta aaataaccat aaaggataag cgcagatatt
    12961 actcaaactc attccaggaa cgaccatcac gggtaatcat cgccaccgag gcaacgggtc
    13021 cccaggttcc ggcctgatac ggtttcggcg catcattgtc catcgcccac gcctcagtaa
    13081 tggagtctac ccatttccag gcttcttcca cttcatcacg acgtacaaac agtgcctgaa
    13141 taccacgcat ggtttccagc agcagacgtt cataggcatc cgccagatgc gtctgattaa
    13201 aggtttctga atagctcaga tccagcttgg tgatttgcag gttatgtttg tggtcaaggc
    13261 caggaacttt attcagtacc tggatatcca cgccttcatc aggttgcaga cggatagtca
    13321 gtttattctg cggcagatcc tgccacgact ctttaaacag attcagttca ggtgttttga
    13381 aatagaccac gacttcagaa catttggtcg gcagacgttt accagtacgc aggtagaatg
    13441 gcacaccggc ccagcgccag ttatcaatgt cgacgcggat cgccacgaag gtttctgtat
    13501 tgctgctctt gttcgcgccc tcttcttcca gatatcccgg cacttttttg ccctgggcga
    13561 agcccgcagt atattgcccg cgtacggttt tttcgcgtac gttggagcgg tcgatgcggc
    13621 gcagggactt cagtactttc actttttcat cgcggatgct gtctgcgctc aggtcagatg
    13681 gcggagacat cgcaatcatg cagagaattt gtagcaggtg gttctgaatc atgtcacgca
    13741 tctgaccggc tttatcaaaa tagccccagc gcccttcgat ccccacttct tctgccacgg
    13801 taatctcaac atgatcaatg gtgcgattgt cccagttatt cacaaacagg gagttagcaa
    13861 aacgcagcgc caacaggttc agcaccgttt ctttaccaag atagtggtcg atacggtaaa
    13921 cctggcactc ctcgaagtat tcgccaacct gatcattgat ttcctgcgag gtcgccagcg
    13981 acgtccccag cggtttctcc atgactacgc gtgccggttt agcattcagt tttgcctcgc
    14041 caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga
    14101 tacgattttt ttgatccagc atcgcgccga gacggttgaa tgcagcagtg tcattgacat
    14161 cgagattaca aaaatccaga cgtgcactca gggtgtccca taaaccttca tcaatggttt
    14221 ctttcatgaa agtttcgagc gcctcgcgga caactttggt atatgccgct ttatcccagt
    14281 cagcacgccc tacgccgata atccgggtgt ccgggttgag ctgaccggct ttttccagtt
    14341 gatacaggga aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca
    14401 ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt taactaaccc
    14461 ggtacttaag ccagggtata cttgtaattt tcttacggtg cactgtactg cttttacgag
    14521 cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac gggaaaagcg cgcaaagtgc
    14581 ggcaaaactg ataaaaaaat catcgttttc cattcggtaa aaatctgaca ctgatcatgt
    14641 tatgaaaaaa aataacaact tttttatctg cttttgtcat taacacggca cacaggcgta
    14701 atatctgaca aaactgcatt tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt
    14761 ctctttacat catgaatatg ctggaaaaaa tccagtctca gctggaacat ttgagcaaat
    14821 cagagcgcaa agttgccgag gtcattctgg cttcgcccga taacgcgatc cattcgagta
    14881 ttgctgctat ggcactggaa gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca
    14941 gcatggacac gcgcggtttt cctgatttta aacttcatct ggcacagagt ctggcgaatg
    15001 gcactcccta tgttaatcgc aatgtcaatg aagatgacag cgttgaatca tacacaggga
    15061 aaatatttga gtccgcaatg gcaacgcttg atcatgtccg tcattcactg gataaatctg
    15121 ccatcaaccg caccgtcgac ttgctcactc aggcaaaaaa aatcgccttt ttcggattag
    15181 gctcttcagc cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt aatgttccgg
    15241 tggtgtactc cgatgatatc gtgctgcaac gcatgagttg tatgaattgt agcgacggag
    15301 acgtggtggt gctgatttct cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc
    15361 tggctcgcga aaacgacgca atggtgattg ccctcacctc tgcgggtacc ccgctcgccc
    15421 gggaagcaac gctggcaatt accctcgacg taccggaaga tactgacatt tatatgccca
    15481 tggtttctcg acttgcacag ctgaccgtga tagatgtgct ggcgacagga tttactttgc
    15541 gacgcggtgc aaaattcaga gataacttga agcgggtcaa ggaagcgctg aaggaatcgc
    15601 gttttgataa gcagttactt aatttaagtg acgatcgcta aaaacgactg tcactgtcct
    15661 aatcttatac gacatccgaa tgagattaat ttatcgccat cgcggcgtta tttcattcgg
    15721 atttcatgtt caagcaacac ctggttgttt cagtcaacgg agtattacat gtccagaagg
    15781 cttcgcagaa caaaaatcgt taccacgtta ggcccagcaa cagatcgcga taataatctt
    15841 gaaaaagtta tcgcggcggg tgccaacgtt gtacgtatga acttttctca cggctcgcct
    15901 gaagatcaca aaatgcgcgc ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat
    15961 gtggctattc tgggtgacct ccaggggccc aaaatccgtg tatccacctt taaagaaggc
    16021 aaagttttcc tcaatattgg ggataaattc ctgctcgacg ccaacctggg taaaggtgaa
    16081 ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt cgtgcctggt
    16141 gacatcctgc tgctggacga tggtcgcgtc cagttaaaag tactggaagt tcagggcatg
    16201 aaagtgttca ccgaagtcac cgtcggtggt cccctctcca acaataaagg tatcaacaaa
    16261 cttggcggcg gtttgtcggc tgaagcgctg accgaaaaag acaaagcaga cattaagact
    16321 gcggcgttga ttggcgtaga ttacctggct gtctccttcc cacgctgtgg cgaagatctg
    16381 aactatgccc gtcgcctggc acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt
    16441 gaacgtgcgg aagccgtttg cagccaggat gcaatggatg acatcatcct cgcctctgac
    16501 gtggtaatgg ttgcacgtgg cgacctcggt gtggaaattg gcgacccgga actggtcggc
    16561 attcagaaag cgttgatccg tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc
    16621 cagatgatgg agtcaatgat tactaacccg atgccgacgc gtgcagaagt catggacgta
    16681 gcaaacgccg ttctggatgg tactgacgct gtgatgctgt ctgcagaaac tgccgcaggg
    16741 cagtatccgt cagaaactgt tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc
    16801 ccgagcatca acgtttctaa acaccgtctg gacgttcagt tcgacaatgt ggaagaagct
    16861 attgccatgt cagcaatgta cgcagctaac cacctgaaag gcgttacggc gattattacc
    16921 atgaccgaat cgggtcgtac cgcgctgatg acctcccgta tcagctctgg tctaccaatt
    16981 ttcgccatgt cgcgccatga acgtacgctg aacctgactg ctctctatcg tggcgttacg
    17041 ccggtgcact ttgatagcgc taatgacggc gtagcagctg ccagcgaagc ggttaatctg
    17101 ctgcgcgata aaggttactt gatgtctggt gacctggtga ttgtcaccca gggcgacgtg
    17161 atgagtaccg tgggttctac taataccacg cgtattttaa cggtagagta atctcacctg
    17221 ccccggccgc tgatggccgg ggtatttagc aacacataaa ttaggactac ttattcaatc
    17281 caatcataat cacagccccc tgtaaaatga atacagggat agcctgaatg gagatctaaa
    17341 tagcttgcaa gacgtcgcat acccgcttcc tctgcaacac tatgtccaat aactgccatc
    17401 ggtaagccac tatctttgga gtattgtgtc tgcacccatg ttttaattga atcgttaatc
    17461 gtaatataat aatcagcctg atattccata tattgtattg gatcacaaaa acagcctgcc
    17521 ccaatgccaa cagttctgac tactctggct ttatctccat aaaattgaat gccaggaaga
    17581 ttatatattt tcaaattttt ggcaaaacgt ttggtaatgt taatggcact gtcagggtct
    17641 atagcatata cacagtacat ctcatcagaa gcaacaatat ttgtaacatc cagttctaac
    17701 tgctgagcca aagccttaga cattccgaac gtaggctctc gatccatgac atcatggcaa
    17761 cgaatgatta ccaaattatt tttatgcaaa aactcctttt tttgctcaat tatttttaaa
    17821 taactgagtt caccgcgagt ataatccatt atttttctgg cccatgaaaa ctcaagatcg
    17881 tcaccctcca g
//
LOCUS       DAJVYM010000080        17884 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVYM010000080 DAJVYM010000000
VERSION     DAJVYM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.080"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="OQS60_002481"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="OQS60_002481"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS2388208.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2589)
                     /gene="hupB"
                     /locus_tag="OQS60_002482"
     CDS             complement(2317..2589)
                     /gene="hupB"
                     /locus_tag="OQS60_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS2388209.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="OQS60_002483"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="OQS60_002483"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS2388210.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="OQS60_002484"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="OQS60_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS2388211.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="OQS60_002485"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="OQS60_002485"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS2388212.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="OQS60_002486"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="OQS60_002486"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS2388213.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="OQS60_002487"
     CDS             complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="OQS60_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS2388214.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9873..10451
                     /locus_tag="OQS60_002488"
     CDS             9873..10451
                     /locus_tag="OQS60_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2388215.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10495..11970
                     /gene="ampG"
                     /locus_tag="OQS60_002489"
     CDS             10495..11970
                     /gene="ampG"
                     /locus_tag="OQS60_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS2388216.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12431..13378
                     /gene="cyoA"
                     /locus_tag="OQS60_002490"
     CDS             12431..13378
                     /gene="cyoA"
                     /locus_tag="OQS60_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS2388217.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13400..15391
                     /gene="cyoB"
                     /locus_tag="OQS60_002491"
     CDS             13400..15391
                     /gene="cyoB"
                     /locus_tag="OQS60_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS2388218.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15381..15995
                     /locus_tag="OQS60_002492"
     CDS             15381..15995
                     /locus_tag="OQS60_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS2388219.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15995..16324
                     /locus_tag="OQS60_002493"
     CDS             15995..16324
                     /locus_tag="OQS60_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS2388220.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16336..17226
                     /gene="cyoE"
                     /locus_tag="OQS60_002494"
     CDS             16336..17226
                     /gene="cyoE"
                     /locus_tag="OQS60_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS2388221.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17411..17722
                     /locus_tag="OQS60_002495"
     CDS             17411..17722
                     /locus_tag="OQS60_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS2388222.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
BASE COUNT         3966 a         4734 c         4371 g         4813 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcggcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ttcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt
     9121 cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg
     9181 gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc
     9241 aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt
     9301 cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag
     9361 tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac
     9421 ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat
     9481 tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt
     9541 tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca
     9601 tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt
     9661 taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac
     9721 acaggcctcg catcagcgtt tcttttacca ggggtgatta tttcgccaac atgatgaatt
     9781 tacatgcctt acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc
     9841 aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag
     9901 ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga
     9961 cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg
    10021 ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc
    10081 tgaccgcctc ccgagatctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg
    10141 cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac
    10201 tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg
    10261 ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca
    10321 acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc
    10381 tgaccgatac catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga
    10441 acgcgcgtta atttctgccc tctggcccgg tgcaaaccgg gcctgtagac gcccatgtcc
    10501 agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt
    10561 gcttccgggc taccgctcgc cctgacatcc ggtaccttac aggcctggat gacggtcgag
    10621 aatattgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa
    10681 ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt
    10741 tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa
    10801 cctggcaccc aactccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctcggcc
    10861 tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt
    10921 gcgggcgcgg caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta
    10981 gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg
    11041 ctgttgatcc cttgtattat cgcgacgttg cttgcaccag aaccgaccga caccattcct
    11101 gtgccaaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat
    11161 aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg
    11221 agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg
    11281 gttaacaaaa cgcttggctt actggcgacc attgtgggcg cattgtacgg tgggattttg
    11341 atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct
    11401 aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc
    11461 gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc actgttaatg
    11521 acgctgtgta ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct
    11581 gtaggacgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg
    11641 acattctatc tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc
    11701 cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg
    11761 acaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg
    11821 ttactgctgt tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg
    11881 ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat
    11941 ctggctctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca
    12001 ctcgccgtaa ttattacggc taaataaaca tcagtatcgc ttaataatat ttgttctttt
    12061 gtgcggctta gcgtttggaa attattgacg caatttatga attctatttt ttcttacacg
    12121 attcagctaa tgagtcttta ttttcccccc acccaattgt caccataatg ataattattt
    12181 gttaaacaat tgttttattc aagattggtt ataccaattg cccgtccaaa ctccttctgc
    12241 actccccttt cgttataacg cccttttgca acagcttctt aaaatcaact tgatatgttt
    12301 tgcaacatat gtaacctgac agccaaatcc aagtaacagg aatttaatca tgtttacagt
    12361 aatgtaacct ccccgtaaaa tgcccacaca ctttaaacgc caccagatcc cgtggaattg
    12421 aggtcgttaa atgagactca ggaaatacaa taaaagtttg ggatggttgt cattatttgc
    12481 aggcactgta ttgctcagtg gctgtaattc tgcgctgtta gatcccaaag gacagattgg
    12541 tctggagcaa cgttcactga tactgacggc atttggcctg atgttgattg tcgttattcc
    12601 cgcaatcttg atggctgttg gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa
    12661 gtacagcccg aactggtcac actccaataa agtggaagct gtggtctgga cggtacctat
    12721 cttaatcatc atcttccttg cggtactgac ctggaaaacc actcacgctc ttgagcctag
    12781 caagccgctg gcacacgacg agaagcccat taccatcgaa gtggtttcca tggactggaa
    12841 atggttcttc atctacccgg aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc
    12901 gaacactccg gtgtacttca aagtgacctc caactccgtg atgaactcct tcttcattcc
    12961 gcgtctgggt agccagattt atgccatggc cggtatgcag actcgcctgc atctgatcgc
    13021 caacgaaccc ggtacttatg acggtatctc cgccagctac agcgggccgg gcttctcagg
    13081 catgaagttc aaagctattg caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa
    13141 agcgaaacag tcgccgaaca ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc
    13201 tagcgaatac aaccaggtgg aatatttctc caacgtgaaa ccagacttgt ttgccgatgt
    13261 gattaacaag tttatggctc acggtaagag catggacatg acccagccag aaggtgagca
    13321 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
    13381 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
    13441 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
    13501 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
    13561 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
    13621 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct
    13681 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
    13741 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
    13801 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggtgtgat
    13861 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
    13921 accgctatcg ggaatagagt acagtccggg agtcggtgtc gattactgga tatggagtct
    13981 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
    14041 gatgcgcgca ccgggcatga ccatgttcaa gatgccagta tttacctggg catcactgtg
    14101 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
    14161 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
    14221 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
    14281 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
    14341 ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca
    14401 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
    14461 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
    14521 catcgtgttc cattctgcga tgctgtggac catcggtttt atcgtcactt tctcggtggg
    14581 cgggatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
    14641 gttcctgatt gcgcacttcc ataacgttat catcggcggc gtggtcttcg gctgcttcgc
    14701 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaatgaaa cctggggtaa
    14761 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
    14821 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
    14881 gctgatgatt gcagctagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
    14941 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
    15001 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
    15061 tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
    15121 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
    15181 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
    15241 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
    15301 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
    15361 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
    15421 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
    15481 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
    15541 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
    15601 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
    15661 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
    15721 ggatggaaat ctacgaattc catcacctga ttgttaacgg catgggtccg gatcgcagcg
    15781 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc
    15841 ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg cggcctgacc agcactaacc
    15901 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
    15961 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
    16021 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
    16081 ggtgattccg ttctggatgg tgatgacagg agctgcctct ccggccgtaa ttctgggaac
    16141 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
    16201 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
    16261 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
    16321 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
    16381 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
    16441 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
    16501 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
    16561 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctaggcttt
    16621 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
    16681 ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga aacgccactc ggtctacggc
    16741 acattgattg gttcgctctc cggcgctgcg ccgccggtga ttggctactg tgcagtaacc
    16801 ggcgagttcg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
    16861 cactcctatg ccatcgccat tttccgcttt aaggattatc aggcggcaaa cattccggta
    16921 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
    16981 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
    17041 gtcgccgcgg cggtgagcgt ctggtggtta ggtatggccc tgcgcggtta taaagttgct
    17101 gatgacagga tctgggcgcg caagctgttc ggcttctcta tcatcgccat cacagccctc
    17161 tcggtgatga tgtccgttga ttttatggtg ccggactcgc atacgctgct ggctgctgtt
    17221 tggtaacaca acctctctat taaaaaggtg ctacggcacc ttttttattg tctgagatca
    17281 gagatacggc taataaccgt atgtcttgac agagtgagca gatgaacata aattaatcat
    17341 acggttgata gccgtattat gtatgagatc gactccctac gatttcattc agttaatcaa
    17401 agaggtcata atgcctggaa aaacagccac tctacccgat gtcgataaaa cgctgaaaaa
    17461 tgcgcgggtc gaactcaaaa ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca
    17521 agccgttggg gtagatttaa gcgcttttat tttgagcgct gctatggaac gcgccgaaag
    17581 tgtgcttgat aaccaacgtc gccgtgagct ttcgaatcaa agctgggaac tgatgaacca
    17641 actcatcgct gaacctgctc aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa
    17701 cagcgatggt cgacaagcat gaagagatta ctctgcccat agtcctctcc tgtaattatc
    17761 agtctgatat tacttatcct gggcaaaaac agtttgattg cggtaaccct gttatcgata
    17821 aatttgtacg cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac
    17881 ttat
//
LOCUS       DAJVYM010000081        17733 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVYM010000081 DAJVYM010000000
VERSION     DAJVYM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.081"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..920
                     /locus_tag="OQS60_002496"
     CDS             <1..920
                     /locus_tag="OQS60_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS2388223.1"
                     /translation="PLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLA
                     HTVVLLNSGGDATQTAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWA
                     KHKLTGGGEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLY
                     RLIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVER
                     TTWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFV
                     LPPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            921..2570
                     /locus_tag="OQS60_002497"
     CDS             921..2570
                     /locus_tag="OQS60_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS2388224.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            2575..3351
                     /locus_tag="OQS60_002498"
     CDS             2575..3351
                     /locus_tag="OQS60_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS2388225.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(3425..4609)
                     /gene="atoB"
                     /locus_tag="OQS60_002499"
     CDS             complement(3425..4609)
                     /gene="atoB"
                     /locus_tag="OQS60_002499"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCS2388226.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(4640..5962)
                     /locus_tag="OQS60_002500"
     CDS             complement(4640..5962)
                     /locus_tag="OQS60_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCS2388227.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(5959..6609)
                     /gene="atoA"
                     /locus_tag="OQS60_002501"
     CDS             complement(5959..6609)
                     /gene="atoA"
                     /locus_tag="OQS60_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCS2388228.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(6609..7271)
                     /gene="atoD"
                     /locus_tag="OQS60_002502"
     CDS             complement(6609..7271)
                     /gene="atoD"
                     /locus_tag="OQS60_002502"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCS2388229.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(7467..8852)
                     /gene="atoC"
                     /locus_tag="OQS60_002503"
     CDS             complement(7467..8852)
                     /gene="atoC"
                     /locus_tag="OQS60_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCS2388230.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(8849..10675)
                     /gene="atoS"
                     /locus_tag="OQS60_002504"
     CDS             complement(8849..10675)
                     /gene="atoS"
                     /locus_tag="OQS60_002504"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCS2388231.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            10842..13691
                     /gene="rcsC"
                     /locus_tag="OQS60_002505"
     CDS             10842..13691
                     /gene="rcsC"
                     /locus_tag="OQS60_002505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS2388232.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(13891..14541)
                     /gene="rcsB"
                     /locus_tag="OQS60_002506"
     CDS             complement(13891..14541)
                     /gene="rcsB"
                     /locus_tag="OQS60_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS2388233.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(14558..17230)
                     /gene="rcsD"
                     /locus_tag="OQS60_002507"
     CDS             complement(14558..17230)
                     /gene="rcsD"
                     /locus_tag="OQS60_002507"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS2388234.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4331 a         4336 c         4629 g         4437 t
ORIGIN      
        1 ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt
       61 tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg gcaaacagca
      121 atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct acccagacgg
      181 ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg ctggccatga
      241 actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg cctgcgggcg
      301 catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt ggtcagggtg
      361 tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc
      421 gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa cgccagttgt
      481 atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg accagcaatg
      541 agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag caggatgcag
      601 ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt gagcgcacaa
      661 catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa ttgctggaaa
      721 aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa
      781 cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg
      841 ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg agtgaatgga
      901 ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg gcgttggtta
      961 ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt gcgcagcacg
     1021 atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta cctgactcgc
     1081 tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg gaggataccc
     1141 atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc
     1201 aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg ctctattttg
     1261 cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa cagcgtcagg
     1321 cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta acggttaaat
     1381 cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa gtcttactgg
     1441 atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt cgggtacggg
     1501 tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt ggttttgctg
     1561 gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc aaaacggtac
     1621 taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag gttgccagtg
     1681 ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc acagcggaaa
     1741 agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg acgtttacta
     1801 atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc gaaaagggca
     1861 aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg
     1921 aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata cgtacttttt
     1981 tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc agcgccacgc
     2041 agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct tggacgcagg
     2101 atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc gaagggacac
     2161 tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac cagatactcg
     2221 cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca acctgccagt
     2281 tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg cgtatattac
     2341 aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt
     2401 tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt acgcttcagg
     2461 atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat cccaccgggc
     2521 ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa tgctatgcga
     2581 aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag tgaaggtgtg
     2641 caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg cgaagatgcc
     2701 agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga tgatcaaaat
     2761 atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa agttcagcag
     2821 ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga tggttcattt
     2881 gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag cccggatggg
     2941 caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat tcgtgcacgt
     3001 ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca tgttgtcacg
     3061 cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg tgcactggat
     3121 atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc agttcacggg
     3181 cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt gacgaccgcc
     3241 caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac atttattgta
     3301 ccgatgcgta atgctggcga gttgacgctg gtgaaaagtt ttgactggtg attaatgggg
     3361 ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg ggtgttttta
     3421 ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca atgcacagtg
     3481 ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg accagaatac
     3541 gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg acattcactt
     3601 tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca aatgcttcat
     3661 tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac gctttttgcg
     3721 tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg gcataacttt
     3781 taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc ataatcacca
     3841 gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt cctgctttat
     3901 cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc gggaattcgt
     3961 cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg atttcggctg
     4021 taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc gccagttcat
     4081 cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta atccccatat
     4141 gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa acctgtccgt
     4201 ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct aaactcatat
     4261 tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc tgggcggcaa
     4321 gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg cacaccgttt
     4381 ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac ccggcttgca
     4441 acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca cgttcaatgg
     4501 cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt gaaccgttaa
     4561 aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt ttatattcct
     4621 tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg acgccggaga
     4681 agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg gcgattgcca
     4741 gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg taggcgatcg
     4801 ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc acgaaaggtc
     4861 cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt gcagaactaa
     4921 aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat tcggtaatga
     4981 gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag gggaattgca
     5041 ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa gccattggcg
     5101 ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg atggtgatgt
     5161 tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg ccgatgatca
     5221 acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc ggtagctgct
     5281 tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg tcagacggtt
     5341 ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca gtgatgaaaa
     5401 tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata tgctcaaccg
     5461 ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca ccccaggtga
     5521 gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg actcgccggg
     5581 cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg atgacacaag
     5641 cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt gcggcggagg
     5701 cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga ccggtaacga
     5761 tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca ccccacattt
     5821 tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat gttagcaatg
     5881 tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa cgcgtcataa
     5941 aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg cggcgacttc
     6001 aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg caatttcggt
     6061 gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca gcatatgcac
     6121 cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg aaccatcttt
     6181 ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat ccatcgcgcc
     6241 acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg cttcttcgtc
     6301 tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta gcgcaaatga
     6361 catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc cgccagcgtt
     6421 caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt tttccgattg
     6481 cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta aaccgatccc
     6541 taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa tacgttgttt
     6601 cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc accaggggtg
     6661 acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc taccagcgtg
     6721 atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg ataggtcagg
     6781 ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg cagtgggcgt
     6841 tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc tacgacggtg
     6901 ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg aatttgctcg
     6961 attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat gcgccgacct
     7021 gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc attgacgatg
     7081 agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt caggtcgcga
     7141 acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc cataaatccg
     7201 cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa tgtcatcaat
     7261 tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca ggcaatagca
     7321 aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa tatgaaggga
     7381 taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg cgcactgtgc
     7441 aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc gtattcctgg
     7501 agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg ggttcggttt
     7561 ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt aatttcctct
     7621 tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca gactggctga
     7681 cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat cacgacggcg
     7741 cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc ggtaagcagt
     7801 gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact gaatttttgc
     7861 aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg cggcagtatt
     7921 aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc ttttaccatt
     7981 gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat ggtctgatgg
     8041 ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc ttgtagtacc
     8101 agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg ttcaaataat
     8161 ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa cagttcactt
     8221 tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc ctttgcccgc
     8281 cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct ttcaccgcta
     8341 atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat gtccatcatc
     8401 gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc ctggtgcaga
     8461 tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat taaattcaac
     8521 tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag cgcttcgacg
     8581 gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt ctcatggctg
     8641 cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc catcaacacc
     8701 acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt cgcacaatgt
     8761 gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt atcttcatca
     8821 tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc gggttgatcg
     8881 gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga atatcaccct
     8941 gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct gaggctttgg
     9001 tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca cagccgttgt
     9061 cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg cgaattttcc
     9121 ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc tgtttgagca
     9181 gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg aagtcgaccc
     9241 gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat gcattgaggc
     9301 tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc tgaataactt
     9361 tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt attgggtcac
     9421 tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga ttacgtactt
     9481 catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct tgcgccatgc
     9541 ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca cctatcattt
     9601 caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg cggtcacgac
     9661 ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta tccagtactg
     9721 gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc agttcatggc
     9781 gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca ccctggcggt
     9841 caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt gtccgcgttt
     9901 cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt tcaccgggca
     9961 attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg gtgatgatat
    10021 caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt atcagcaaat
    10081 cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg cgaatatctt
    10141 cggttaattc attggcccag atatagccga ggatttcacc attacgctca atggggagca
    10201 ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg gtatttgtac
    10261 gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta ttctgatata
    10321 gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa ccagcaccta
    10381 tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca tttaatgcgc
    10441 ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct agtgcctgat
    10501 taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact gctgaacgtc
    10561 cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc aggattgcca
    10621 tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa tgcatgctga
    10681 aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat gatttctcgg
    10741 cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta ccgctacctt
    10801 aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt
    10861 ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta gtgctctggc
    10921 tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag cgagaatcgg
    10981 aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt caacgcacct
    11041 ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg
    11101 tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg
    11161 ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag tcattggcgt
    11221 ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac cgggtatttt
    11281 taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg ccagtggaac
    11341 gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat gcaccacaag
    11401 atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc gtcgggtatt
    11461 tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg ggtgtcgagc
    11521 agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg gttaccattc
    11581 ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa attaagggcg
    11641 atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc cgggagctgg
    11701 tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg ccggttgata
    11761 aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg
    11821 gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt ccggcggaaa
    11881 gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc gcctccgcgc
    11941 cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt aacgaactgg
    12001 cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg caaattatct
    12061 gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat ctgcaaatca
    12121 gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt
    12181 cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg gaacaggcga
    12241 gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg
    12301 gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcggc
    12361 tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc gatattctcg
    12421 atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg
    12481 aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag cagttaggct
    12541 tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg atgcgtttac
    12601 agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc ggctgtatag
    12661 ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat accggcgtgg
    12721 ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg
    12781 tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca
    12841 tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag tttaccgtgc
    12901 gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg ttgagtggta
    12961 aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa accagtttgc
    13021 agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc gaagatgtgt
    13081 taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg accttctgtc
    13141 gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac agtgtggctg
    13201 ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg gagagcgacg
    13261 atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac gatatgatga
    13321 ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag ttgggatcgt
    13381 tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta cttagcaaga
    13441 atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt taccgcttga
    13501 cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact gctaatgcgt
    13561 tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg tcgaagccgg
    13621 taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc aggaaatcgc
    13681 gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc aaatgccaga
    13741 tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa taatgggaat
    13801 cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc cagataagac
    13861 actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac ttaacgtcac
    13921 tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca tcatcgcaga
    13981 tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga tctcggtcac
    14041 caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga gacgcttgtc
    14101 accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg gggtaaattt
    14161 cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac cttgtttcag
    14221 cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt tgttgttcat
    14281 agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga ttaaggtaat
    14341 gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat gcgcatccag
    14401 tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca
    14461 ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt catcggcaat
    14521 aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc ttgacataac
    14581 tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga atcagatgtt
    14641 ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat acgcctttaa
    14701 gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatacagcc
    14761 tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt
    14821 gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt
    14881 caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg acgcacaatt
    14941 gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag
    15001 taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat
    15061 cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt
    15121 ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct tcttcctctt
    15181 caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta ccaagcccat
    15241 cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg ctcagccaga
    15301 atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata aacgggaagt
    15361 gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg cggaatgtca
    15421 ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg
    15481 aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg cgatcgccgc
    15541 ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga
    15601 tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag aacagcaccg
    15661 tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc
    15721 gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg agtttagccg
    15781 cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt ttcataaagg
    15841 tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta
    15901 ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcgacctggc
    15961 tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat
    16021 gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa tgatcggcaa
    16081 ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccgagcggca
    16141 gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg
    16201 tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta tagccaaata
    16261 acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt tgcaacgtat
    16321 ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta gagttgagtg
    16381 ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc gttccttctt
    16441 tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa ctgtccagcg
    16501 gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat
    16561 gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc caggccaggc
    16621 ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc aacatttctg
    16681 cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag gtgagatctt
    16741 tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg taatacatcg
    16801 accacggtac attttctgca ccccacaatg tatccagata agtggacatc cgctgagtca
    16861 tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg cggcgtggtt
    16921 tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg
    16981 gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga
    17041 cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct ttcagccagg
    17101 cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg atcaacagta
    17161 aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt
    17221 tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag
    17281 ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct cccgtgatat
    17341 ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct
    17401 acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat tatgctgatg
    17461 ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc acagaataat
    17521 gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg aaataggggt
    17581 aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat agcgggagat
    17641 attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata cttaagaata
    17701 agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAJVYM010000082        17725 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVYM010000082 DAJVYM010000000
VERSION     DAJVYM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.082"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..183)
                     /locus_tag="OQS60_002508"
     CDS             complement(<1..183)
                     /locus_tag="OQS60_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2388235.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLT"
     gene            complement(393..911)
                     /locus_tag="OQS60_002509"
     CDS             complement(393..911)
                     /locus_tag="OQS60_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS2388236.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1608..2108
                     /gene="tssB"
                     /locus_tag="OQS60_002510"
     CDS             1608..2108
                     /gene="tssB"
                     /locus_tag="OQS60_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCS2388237.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2143..2367
                     /locus_tag="OQS60_002511"
     CDS             2143..2367
                     /locus_tag="OQS60_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388238.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2418..3893
                     /gene="tssC"
                     /locus_tag="OQS60_002512"
                     /pseudo
     CDS             2418..3893
                     /gene="tssC"
                     /locus_tag="OQS60_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            3900..4313
                     /gene="tssE"
                     /locus_tag="OQS60_002513"
     CDS             3900..4313
                     /gene="tssE"
                     /locus_tag="OQS60_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS2388239.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4317..6167
                     /gene="tssF"
                     /locus_tag="OQS60_002514"
     CDS             4317..6167
                     /gene="tssF"
                     /locus_tag="OQS60_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS2388240.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6131..7213
                     /gene="tssG"
                     /locus_tag="OQS60_002515"
     CDS             6131..7213
                     /gene="tssG"
                     /locus_tag="OQS60_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCS2388241.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7238..8518
                     /gene="tagH"
                     /locus_tag="OQS60_002516"
     CDS             7238..8518
                     /gene="tagH"
                     /locus_tag="OQS60_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCS2388242.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8515..9039
                     /gene="tssJ"
                     /locus_tag="OQS60_002517"
     CDS             8515..9039
                     /gene="tssJ"
                     /locus_tag="OQS60_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCS2388243.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9042..10371
                     /gene="tssK"
                     /locus_tag="OQS60_002518"
                     /pseudo
     CDS             9042..10371
                     /gene="tssK"
                     /locus_tag="OQS60_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10376..11137
                     /gene="icmH"
                     /locus_tag="OQS60_002519"
     CDS             10376..11137
                     /gene="icmH"
                     /locus_tag="OQS60_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCS2388244.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGDEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11146..13923
                     /gene="tssH"
                     /locus_tag="OQS60_002520"
                     /pseudo
     CDS             11146..13923
                     /gene="tssH"
                     /locus_tag="OQS60_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            13920..14663
                     /gene="tagO"
                     /locus_tag="OQS60_002521"
     CDS             13920..14663
                     /gene="tagO"
                     /locus_tag="OQS60_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCS2388245.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14668..16080
                     /gene="tssA"
                     /locus_tag="OQS60_002522"
     CDS             14668..16080
                     /gene="tssA"
                     /locus_tag="OQS60_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCS2388246.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16099..>17725
                     /gene="tssM"
                     /locus_tag="OQS60_002523"
     CDS             16099..>17725
                     /gene="tssM"
                     /locus_tag="OQS60_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCS2388247.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         4131 a         4852 c         4850 g         3892 t
ORIGIN      
        1 cgtcaggtag gccattttgt ccagtacctg cggaaattca agggagagaa actgctggct
       61 gaccagagag agctcgaggg aaaagagcga agagagtgac tggttcagat gaaaggagac
      121 caccgcaaaa gcatccggtg gcaggccgtc cacttccagc gtgaaacgta atccggttga
      181 catagcaacc tccggggtta aggaaaaaca gacagttcgc tgttaaagcg gctgtgacag
      241 tacagtgtta cgcttgcgtt caggcacgct accgccgaag tttccggcat ccgccatctc
      301 ccgtgaacgt aaccccttac tgacaacctt tctttctacg gcgtaataca aaaacagcat
      361 ccggcctgat agccggatgc tgttggctta acttatgctt ccagtggtgc gcgccagtcg
      421 tcggcaccgg aggtgccaga tttgacgtgt tcccattcaa ttttgcggta ggccagtgaa
      481 acctcgagca actgggtgaa ctcgcgtttc gccgggtcct ggcaatgcgg catatgcagt
      541 ttcatatcga caatcgtcga atcagtcagg cgggtagtga aataatgctc ctgtttgccc
      601 tccacagagg tacgccacca gtgaagttca gtggttggca gcatttcgcc ggaggccagt
      661 gcgttgtaca tcagcggaac cgctttgttc agcgccacgg taaaaatgaa aggcttgtga
      721 gcacgctgac ccgcaggctg accggactga ggagtggtcg ggacagtaac gttatgcagg
      781 aattcctgca ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc gacagaatca
      841 gcggtaaatg cacctgcagt aatatttccc tgggtctgac cagtgatgga gatataacac
      901 ggtgttggca tttgtaaact ccttgttaac gtggttaatt actacttaca atgacctgcc
      961 gttaatccca gacaggcaga tgcagctcac ttcacggaac cgcatcattt ttcagtattt
     1021 tttacataca aaccgagcta gaatttacgg ttgtcgtaaa aactattttt aaaatcatta
     1081 aatctcttat tcagcaataa cttaataata tatatctgga taaccatacc caactgttca
     1141 aagtccgtat tgcctccata aaatggccaa agctgaatca tgaataaata ttgtatttat
     1201 cctttctgaa ttatcatttt gataattttt gagatattcc ttaaaaatac ccacttaatc
     1261 aatcacaaaa caaatatata aacaagcaat cattagatta gcttattttt aatgaaatta
     1321 ttatatactt caataagtta cacaaaaact ctatatttaa attattgcca taatacacct
     1381 gaagcgaatt gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg
     1441 atcgataaaa agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag
     1501 tggtgatttt attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa
     1561 tatgccgtgt tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat
     1621 ttgaaggcag cgtcgcgccc cgcgagcgta ttaatatatc gtatgtccca aaaacagatg
     1681 gccagacggc tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata
     1741 cgcaggaaac atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg
     1801 gtgcagtgat ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg
     1861 gaagcacaac agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt
     1921 caccagatag cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag
     1981 cactgactgc actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg
     2041 cattgctgga aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca
     2101 ataaataacc accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat
     2161 atctccggga cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt
     2221 ttcagcccgt ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat
     2281 cgccgtatca gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg
     2341 tcgcactggc agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa
     2401 acagaaggat tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc
     2461 agcctgagca ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg
     2521 gttcggaagg ttatgacgtt gccctccagg gtgtgacagc ctttattgcc agcatcctgc
     2581 aatccactgc atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata
     2641 ttgatgaacg catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg
     2701 tcgagtcgtt ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca
     2761 tcaaggtcaa cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc
     2821 cagaaattat tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg
     2881 gcggtaaacc catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca
     2941 tgaaactgct gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct
     3001 ccgtttcccc ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc
     3061 tttatgccat ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag
     3121 attcgcgcta tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa
     3181 cagataaccc tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt
     3241 atctgtgggg taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat
     3301 accgctgatg tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccga
     3361 tgcatctgtt cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca
     3421 ccgatcgccg cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag
     3481 actctgataa tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg
     3541 gaaaagatgc agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca
     3601 atcggttagc gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa
     3661 ggagcgactt agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa
     3721 acccgcctgc tgacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg
     3781 atgtagaagg cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat
     3841 ttatgggggc aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca
     3901 tgataccgga tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg
     3961 ccagaaaccg gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac
     4021 gaaacattct taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg
     4081 aaccggaagg ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag
     4141 aacgctatga accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca
     4201 gcccgctgga tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg
     4261 tgcttgaatt tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg
     4321 aatttgaaga acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc
     4381 tggcaacgga aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata
     4441 ttgaacgcct gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg
     4501 atgaattccc agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc
     4561 cggttccggc aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg
     4621 tgttaatccc ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag
     4681 tgctgccctc tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg
     4741 atatctggct gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg
     4801 gtgttatcaa catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga
     4861 acaaacttcg cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat
     4921 ggttctgcga atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc
     4981 ctgagtttat gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga
     5041 aaaacgtcca cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc
     5101 tcttttttga tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta
     5161 ccctgcaact gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc
     5221 tgcgcctgta ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc
     5281 tggacaaccg gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg
     5341 atgtattttc cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc
     5401 tggggcgtcc tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc
     5461 atcagataga atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat
     5521 ccctgttcca tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc
     5581 cgtcagacga atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc
     5641 gtgagcttcc gtcacaaatt cgttccggcg atatcaccgg cacaaccgga aaaaatgcgg
     5701 ctgttgcttc atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg
     5761 gcagcctgca ctggccccta ctctccgcca tgaacctgaa ttatctgtca ttactggata
     5821 cggacgcgct gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga
     5881 cggcacggct gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg
     5941 atcgcctgtt tacgggtatt cccgtccggg ggctggcttc cacgctgtat ctgcacccgg
     6001 agccgtttgt ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt
     6061 cgctgtacgc cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg
     6121 agacatggaa atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc
     6181 cccggcagat aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta
     6241 caattttttt gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc
     6301 agaattatcc ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag
     6361 ccttgccttc ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag
     6421 gatgaccacc acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct
     6481 cgctcacctg gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat
     6541 gttcagccac cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat
     6601 cagttttcgt aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct
     6661 gggccaccgc cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc
     6721 cgggatcctg gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg
     6781 ctttgatttg tcagaagtga cactgcaaaa ctggcaacgt cgcaaggtgg atattgaacc
     6841 ggatcagcaa aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag
     6901 atcggtactg ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca
     6961 gctcagtatt accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa
     7021 cttcctgcca ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt
     7081 acatctgggg ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc
     7141 gctgctgggc tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag
     7201 ggtacggtcg taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac
     7261 gctttcattg caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct
     7321 gttcacacag cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg
     7381 tcagcagagc attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct
     7441 gcgcccgcag acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct
     7501 aatacagttg cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct
     7561 gaaccgggga gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac
     7621 caatcgcgat atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg
     7681 aatgactcac cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact
     7741 ccaggctctt cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc
     7801 tgtccggcca tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt
     7861 tatggatctg ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc
     7921 agaaatggca caacgccacg ttgcggtcac ccccttactg cgcggtcttg gcggctcgct
     7981 taccatgagc aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca
     8041 ggccgcaata aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca
     8101 tttgcgcccg ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga
     8161 cgtaatgttt gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga
     8221 aagcctgcgc aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct
     8281 tcgtgtcctg ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg
     8341 ccgcagccat gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca
     8401 ttattacgaa gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt
     8461 ctacgcccag gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac
     8521 aggaaggcgt ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg
     8581 aaaaaaatca gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa
     8641 ccttcggaaa taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt
     8701 gaatcagccg ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca
     8761 gccgactatg accagatagc cagcacgccg ctaccggatg tgctgggtaa aaactatatc
     8821 gaccaccagg atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg
     8881 gatgagaaaa cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa
     8941 tggaaacaaa tcgagacggt ggaaggaatc ggccaccatt accgcctgct ggtgcatgta
     9001 cgtcaaagca gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca
     9061 aggtgatgtg gcaggaaggg atgctgatgc gtccacacca tttccagcag cagcagcgtt
     9121 acaacgacta cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta
     9181 ccgaactcac cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt
     9241 caggcacact gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc
     9301 cgctgcaccc gcaaaatttt ccggacgaaa gaagccgcaa tatctacctc gctctgccgg
     9361 tcgccagtga tgtgagaaat gaaatcagcg acggacggcg aatcgggcgt taccggctga
     9421 attatgccga tgtccgggat ttgcattcag aagaaggtga cgcgcgaaca ttgacgctgg
     9481 gtcaactgac gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc
     9541 cgctttgccg tatcagcgat cgccgccgat ggctccctga cgttggatga cgattttatc
     9601 ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag
     9661 ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc
     9721 ggcatcgcgg atgtggcgga atttatgatg ttgcagttac ttaaccgtaa ccagacccgg
     9781 tttacccatc acgcccgtcg atcccagctc cacccggaag atttctacct tgatcttgcc
     9841 gggttgctgg gtgaactgat gacctttacc gagccgtcgc gcctgccctg cccgcttgat
     9901 gtgtatgatc atcatgacct gaccaaaata tttaaaacgc tgttaccgga agtcaaacgg
     9961 gcgctgcata ccgtactgtc gccaagagcc gtcaatctgc cgctgcatct gcgtgacggt
    10021 atctggcagg ccgatatcca tgacacagaa ctgctgcaat ctgccacctt tgtgctggcc
    10081 gtggcggcaa acatgccggt cgatcagatc cagcgccagt ttatccagca gtcgaaaatt
    10141 tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tccccggtat tccattgcgt
    10201 gcccttatgg tggctccccg ccagcttcct taccattccg ggttcagcta tttcgaactc
    10261 gacaagagtg gccaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgtt
    10321 tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga
    10381 tgaggggtca ctgtctctgc caccatttac cggacacgac gaaaaaagcc agcgcaacta
    10441 ccatctcgcg cttcgtggca acagcctgaa tccgatgatc gacgcggcca cgccgctact
    10501 cggtatggta atgcgccttt caaccatgaa cagccagacg atgccggagc atctttttgc
    10561 tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggagcagg gctacgaacc
    10621 gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg
    10681 taacggctgg tcaaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa
    10741 cgaagcctgg ggtgacgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa
    10801 acgttaccag gatctgctgg aatttctctg gatctgtttt tctctgggat ttcgcgggcg
    10861 ctacaaagtg gcagcacagg atcaggggga atttgagcag atctaccgcc gtctgtacca
    10921 cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac
    10981 ccagggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt tttgtggtgg
    11041 cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca
    11101 ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat ccagattgat
    11161 cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc
    11221 tctgaatgta tgagtcagca ggcagcggaa attaccgtca gccatgtgct cattcagatg
    11281 ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat tggcatggaa
    11341 gagttgcgcc aggcgctgac ggtagagaac tacacaaccg cccgttctgc ggacagctac
    11401 ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctagctgct ggcgtcggct
    11461 gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccctgct gcattcgccg
    11521 ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg
    11581 caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac
    11641 ggtaaaggcg caggcacaat gacggacgcc tctgacaccc tgcttgcccg ctatgccaaa
    11701 aacatgaccg cagacgcccg taacggcagg cttgacccgg tactgtgccg cgaccacgaa
    11761 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccggt ggtggtgggc
    11821 gaagcgggcg tgggcaaaag cgcactgatt gaagggctgg cgctgcgcat cgtggcaggc
    11881 caggtgccgg acaagctgaa aaacaccgat atcatgaccc ttgacttggg cgcattgcag
    11941 gccggggcgt cggtgaaggg tgaattcgaa aaacgtttca aagggctgat ggcggaggtc
    12001 atttcctccc cggtgccggt cattctgttt atcgacgaag cacataccct gattggcgcg
    12061 ggcaaccagc agggcgggct ggatatctcc aacctgctca aaccggcgct ggcgcgcggc
    12121 gagctgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgaaaaagat
    12181 gccgccctgt cgcgccgctt ccagttggtg aaggtcagcg aacccaacgc tgccgaagcc
    12241 accattattc tgcgcggtct gtcggcggtc tatgaacagt ctcacggcgt gctgattgat
    12301 gatgacgcct tgcaggccgc tgcgacatta agcgagcgtt atctctccgg gcgtcagtta
    12361 ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtcg
    12421 tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga ggcggaaatt
    12481 cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag
    12541 gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc ggcctggcac
    12601 cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact gggcgtggca
    12661 gaggacgatg cggcgccgtt gccggacgca gataccgtgg aggatacgca gccagagtca
    12721 gagtcagagt cagagtcaga gcaggataat accggtgccg aaccggctga tgaggccgac
    12781 agagaacaac cggaagagac cgctgaaaca gtttccccgg tacagcggct ggcccagctc
    12841 actgccgaac tggacgccct gcataacgac cggttgctgg tctccccgca cgtcgataaa
    12901 aaacagattg cggcggtgat tgccgaatgg accggcgtgc cgctcaaccg cctgtcgcag
    12961 aatgaaatgt cggtcatcac cgacctgacg aaatggctgg gtgacaccat caaaggccag
    13021 gacctggcga ttgccagcct gcataagcat ctactgaccg cacgcgccga cctgcgtcgt
    13081 ccgggacgcc cgctcggtgc gttcctgctg gctggcccca gcggtgtggg taaaaccgaa
    13141 accgtcctgc aactggcaga gctgctctac ggcggtcgcc agtacctgac caccatcaat
    13201 atgtccgaat tccaggagaa acacaccgtc tcgcggctga ttggttcgcc tccgggctac
    13261 gttggctacg gtgaaggcgg cgtactgacc gaagcgattc gccagaaacc ctactcggta
    13321 gtactgctcg atgaagtgga aaaagcgcac ccggatgtgc tcaacctgtt ctaccaggcg
    13381 ttcgataagg gcgaaatggc agacggtgaa ggccgcctga ttgactgcaa aaatatcgtc
    13441 ttcttcctga cgtccaacct cggctaccag gtaatagtcg agcatgccga tgacccggaa
    13501 accatgcagg aagtacagta tccggtgctg gccgacttct tcaaacctgc cctgctggcg
    13561 cgtatggaag tggtgccgta tctgccgctg tcgaaagaga cgctcgccac cattatcgcc
    13621 ggaaaactgg cccgtctgga taacgtgctg cgcagtcgct ttggtgtaga agtggtcatt
    13681 gaaccggaag tgacggacga aatcatgagc cgcgtcaccc gcgcggaaaa cggcgcgagg
    13741 atgctggaat cggtcatcga tggcgacatg ctaccgccgc tctcgctgct gctgttgcag
    13801 aaaatggcgg ctaacacggc gattgcccgg attcggttgt cggcagtgga cggcgcattt
    13861 acggcagacg tggaagatgc tcagaacgac gagtccgtca caaaggatga aacggtttta
    13921 tgagcaggtt gcgcgttttc cggcagacag gatggtttat cgcgggcctg atgacgggct
    13981 tacccgccac tgccgcgccc gcagaagcat cgtcaatggc cggtgtggcg gtcgccgtcg
    14041 ccaccacgac cccgccggat gcgacagcca cgttacaggc catgcagtcc tgtcggcggg
    14101 agtccgcggc gctggagcgg cttgactgct atgaccgcct gctggcaccg ctgtccccgt
    14161 cagggtttga cggggcgctg gtcaaagccg gttttgtggg cgaggcctgg acccgcgcca
    14221 cagagcagga aaaacgccgc gagggcaaca ccacagaact gctggtgacg caggtgccgg
    14281 gggagcgccc gacggtggtg ataaccaccc cggctatcgg tcatgttccg ccacgcccgg
    14341 tgctgatgtt cagttgcgtg gacaatatca cccggatgca ggtggcgctg atgcaccctc
    14401 tggatgtcca tgatatcgcc gtcactctga atgccgacaa ccgggcattg cgcagccact
    14461 ggtttgtgcg cgagaacggc accctgctgg agtccagccg ggggttatcc gggattgatg
    14521 aaattaagca actgttcggc gcaaaaacgc tgacggtgga taccggcgca gacaatgccg
    14581 ccggaaagct gacctttaac attgacgggc tggcaagggc gattgctccc ctgcgcgatg
    14641 cctgccactg ggcgggagaa taaaaatatg gcaatggact tacgcgatcc gaatgtctgg
    14701 atatcgcacc tgctggaaaa cctgccggaa gagaaactgg catcggcgct gaaagatgac
    14761 aacccgaact gggagtatat cgacggcgaa atcgtcaagc tgggatcgct ggcccacagt
    14821 caactggata tcccggaact ccagcgccgg gggctggtca ttctggcttc ggagagtaag
    14881 gattttcgtc tgctggcgca tctgctaaga accctgcaac acgccggtga tcctcatctc
    14941 gcattgcgcc tgctggcgct gtatgtggag cattactggg cggtggctgc gccgcagaat
    15001 atggcgcata aaaaacgctt tgccagccag gtcattaaac gctttgaaac aggtattgaa
    15061 gttttttcac agaacgccgc cacagcgcag cgcgatgcgc tgtcagggga actggcaaaa
    15121 ctggcgcaat gctggcagtc acataacgcc ccggaactgg cacaggctac tgatgatctt
    15181 tttgccctgt accagcgtgc gtttaatcgt gcggcaccgg ctcctgtccc cactccggcg
    15241 gcctccggta gttcaccaca gaccaccgcc acgtctgaaa gcggcgtgac gcaacccagt
    15301 gctccggctc cccaaatcgt catcgacagt cacgacgaca aagcctggcg cgacacgctg
    15361 ttaaaagtgg cggccatttt atgtgaacgc cagccggatt caccgcaggg ctaccgtctg
    15421 cgccgccatg cgctttggca gaacatcacc agtacgcccc aggcggaaag cgatagccgt
    15481 actccactgg ctgcggtctc cgccgatatg gtggcagatt accatgctca gctcggtagt
    15541 gccgatatgg cgctgtggca gcaggtggag aaaagcgtac tgctggctcc ttactggctg
    15601 gatggtcact gcctttctgc acagacggcg ctgcgtctgg gttacaaaca ggtggcagac
    15661 gccatccacg atgaggtcat ccgcttcctt gagcgtctgc cccagcttac cgggctgctg
    15721 tttaatgacc acacaccgtt tatcagtgag cagacgaaac aatggctggc agcttcgccc
    15781 gacgccaaag ttgcaccggt tgcgcaaatc ggtgaggaat cgaaggcagc cagagcctgt
    15841 tttgctgagc agggtctgga ggcggcgctg cgatatctgg acatgctacc cgaaggcgat
    15901 ccccgcgatc agtttcaccg ccagtacctt gccgcacagt tgacggaaga ggcggggctg
    15961 gtacagcttg cgcagcagca gtaccggatg ttgttcagga tgggattgca gatgatggtg
    16021 gcagactggg agccgtccct gcttgagcag ctagaacaaa aattcacggc agaacaataa
    16081 aagagaacag gagtttttgt gttcagatta ccaacacccc gactactcag cggactcaaa
    16141 tcagccctgc gaccggcgat gccccggttt aaagtctctg ctttctggct gctgatactg
    16201 gcgtggattt ttctgctggt gtggatctgg tggaaaggcc cgatgtggac gctgtatgaa
    16261 gaacagtggc tcaaaccact ggcgaaccgc tggctggcaa cggcggcgtg ggggattatt
    16321 gccctggtgt ggcttaccgt ccgggtgatg aagcgcctgc aacagcttga aaaaatgcaa
    16381 aagcaacagc gcgaggaagc cgttgatccg ctcagcgtgg aactgaacgc ccagcagcgt
    16441 tatcttgacc gctggctgct gcgcctgcaa cgccatctcg acaaccgccg tttcctgtgg
    16501 cagttgccgt ggtatatggt catcggcccg gcgggcagtg gcaaaacgac actgctgcgc
    16561 gaggggtttc cgtccgacat tatttatgcc ccggagggcg cacgcggcgc agaacaacgc
    16621 ctgtacctca cgccccatgt cggtaaacag gcggtgatct ttgatatcga cggcacactc
    16681 tgcgctcccg ctgatgcgga tatcctgcat cgccgtctgt gggaacatgc cctcggctgg
    16741 ctgaaagaaa agcgcgcgcg ccagccgctg aacgggatta ttctgacact cgatttaccc
    16801 gatctgctga ccgcagacaa acgccgccgc gagcatctgt tacagacgct gcgcagccgc
    16861 ttacaggata tacgccagca tcttcactgc cagttaccgg tttacgtggt actgacccgg
    16921 cttgatttat tacagggttt tgccgccctg ttccagtcac tcaacagaca ggatcgcgat
    16981 gcgattctgg gcgtcacgtt cacccgccgt gcccatgaaa atgatgactg gcgaacagag
    17041 ttgaatgctt tctggcagac atgggtggat cgaatgaatc tggcgttgcc ggatctgatg
    17101 gtcgctcaga ctcacacccg cgcgtcttta ttcagttttt cccgccagat gcagggaagc
    17161 cgtgaaccgc tggtgtcact gcttgagggt ctgcttgatg gcgaaaatat gaacgtgatg
    17221 ctgcgtggtg tctatctcac ctcttcgctt cagcgtggac agatggatga tatattcacc
    17281 cagtctgccg cccgccagta ccggctgggc aataacccac tggcgtcctg gcccctggtg
    17341 gacaccgcgc cttatttcac ccgcagcctg ttcccgcagg cattactcgc agagcctaat
    17401 ctggcaacag agagccgcgc ctggctgata cgttcccgtc gccgcctgac ggttttctct
    17461 gccacaggcg gcgtggcagc actgctgctc atcaccggct ggcatcacta ttacaacggt
    17521 aactatcagt ccggcatcac cgtgcttaag caggccaaag cctttatgga cgtgccgcct
    17581 ccgcaggggg aagatgactt tggcaacctg caactgccgc tcctgaaccc ggtacgcgat
    17641 gccacactgg cctatggcga ctggggcgac cgcagccgtc tggccgatat gggactgtac
    17701 cagggacgac gtatcgggcc ttatg
//
LOCUS       DAJVYM010000083        17448 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVYM010000083 DAJVYM010000000
VERSION     DAJVYM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.083"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..502)
                     /locus_tag="OQS60_002524"
     CDS             complement(<1..502)
                     /locus_tag="OQS60_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS2388248.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OQS60_002525"
     tRNA            complement(688..772)
                     /locus_tag="OQS60_002525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OQS60_002526"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OQS60_002526"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS2388249.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OQS60_002527"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OQS60_002527"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS2388250.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OQS60_002528"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OQS60_002528"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCS2388251.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OQS60_002529"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OQS60_002529"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCS2388252.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OQS60_002530"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OQS60_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCS2388253.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OQS60_002531"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OQS60_002531"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCS2388254.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OQS60_002532"
     CDS             7117..8619
                     /locus_tag="OQS60_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS2388255.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OQS60_002533"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OQS60_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS2388256.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OQS60_002534"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OQS60_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS2388257.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OQS60_002535"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OQS60_002535"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS2388258.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OQS60_002536"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OQS60_002536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS2388259.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OQS60_002537"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OQS60_002537"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS2388260.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OQS60_002538"
     CDS             complement(16186..17382)
                     /locus_tag="OQS60_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS2388261.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4484 a         4335 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAJVYM010000084        17294 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVYM010000084 DAJVYM010000000
VERSION     DAJVYM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.084"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            93..1133
                     /locus_tag="OQS60_002539"
     CDS             93..1133
                     /locus_tag="OQS60_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS2388262.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OQS60_002540"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OQS60_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS2388263.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OQS60_002541"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OQS60_002541"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS2388264.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="OQS60_002542"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="OQS60_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS2388265.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OQS60_002543"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OQS60_002543"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS2388266.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="OQS60_002544"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="OQS60_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS2388267.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9087)
                     /locus_tag="OQS60_002545"
                     /pseudo
     CDS             complement(8440..9087)
                     /locus_tag="OQS60_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OQS60_002546"
     CDS             complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OQS60_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS2388268.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OQS60_002547"
     CDS             complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OQS60_002547"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS2388269.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OQS60_002548"
     CDS             complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OQS60_002548"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS2388270.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12900..14081)
                     /locus_tag="OQS60_002549"
     CDS             complement(12900..14081)
                     /locus_tag="OQS60_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS2388271.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14336..15565
                     /locus_tag="OQS60_002550"
     CDS             14336..15565
                     /locus_tag="OQS60_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS2388272.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16025..16657
                     /locus_tag="OQS60_002551"
     CDS             16025..16657
                     /locus_tag="OQS60_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2388273.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16991..>17294
                     /locus_tag="OQS60_002552"
     CDS             16991..>17294
                     /locus_tag="OQS60_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2388274.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3975 g         4227 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgccta ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacatggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggtacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tattccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
     8881 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
     8941 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
     9001 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
     9061 tcctcccagc aaaccaattg ttttcattcc tctctctcct gatgtgtgtt accgcaacaa
     9121 ggctgaataa ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa
     9181 aaaagggccg gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact
     9241 tctcgcctgc catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa
     9301 tgagccagaa agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac
     9361 cggcagcacc aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca
     9421 cccagttcgt ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt
     9481 cagagcacag gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca
     9541 tcgtcatgcc aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca
     9601 tcaggcaata gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag
     9661 ccggtttacg ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg aacataaagg
     9721 tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt
     9781 tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct
     9841 gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc
     9901 aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt
     9961 ccgacgtatc gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg
    10021 ccagccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta
    10081 aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata
    10141 aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc
    10201 gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga
    10261 cagcaatgcc cagcaccacc cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg
    10321 accctataga accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca
    10381 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10441 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10501 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10561 aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg
    10621 tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg
    10681 aacaactcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10741 tgtcgttatg tgatggatat tccaattttc aaattaagtt gaattattga gattattatt
    10801 aaccacctaa ttttacagca gataaaattc ataaagttca ttaattgata attgatatgg
    10861 attatttcat aaccatgata tgaattatga tgacctacag gtataaaaaa ccctgccatg
    10921 aggcagggtc ataaaagtaa gaggaatgaa ttaacgagcc agccaaccgc catctacggc
    10981 aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta tcggccccat
    11041 caggtcactc ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt ccgcgctacg
    11101 ttgttcatct gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc
    11161 attaacatta atgttgtgtt tagcccattc gttcgccatc aatcgcgtca cgcccatcac
    11221 gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11281 cgtgatattg ataatcttgc cgccattgcg ttgcgcgata aagtgtttcg ccgctgcctg
    11341 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11401 gaactcgaga gcatcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11461 accaaactca gctaccgcgc gatccagcag tgctggaatg ccatcaatct ttcgcagatc
    11521 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg
    11581 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11641 ctggcccagt ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc
    11701 acttaaaatc ataacaatac cttattcgtt tatgccgaca actagcgcaa atctttaacg
    11761 gccactatgc cgacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
    11821 cctggttttc accaaccatg ccccagataa aggtataagc tttggttccg acgccggaat
    11881 ggatcgacca gctgggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
    11941 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
    12001 aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag ttacctggag
    12061 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
    12121 tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
    12181 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
    12241 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
    12301 acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc gtaccggcac
    12361 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
    12421 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
    12481 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
    12541 aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
    12601 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
    12661 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
    12721 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
    12781 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
    12841 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
    12901 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
    12961 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
    13021 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
    13081 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
    13141 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
    13201 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
    13261 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
    13321 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
    13381 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
    13441 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
    13501 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
    13561 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
    13621 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
    13681 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
    13741 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
    13801 gctttaagtc cggaaccgca aacgtcatta atcgtgattg cagaaacgct attaggaaga
    13861 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
    13921 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
    13981 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
    14041 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
    14101 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
    14161 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
    14221 gcaaccacta acccaccaat agatgggtag ataaggcgtt aatctcccat acttaacctg
    14281 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
    14341 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
    14401 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
    14461 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
    14521 cctctcatca aaaacatcag catgtgaagg gataacgggc gcggtaacac actactatgg
    14581 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
    14641 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
    14701 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
    14761 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    14821 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
    14881 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    14941 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    15001 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    15061 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    15121 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
    15181 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    15241 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
    15301 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    15361 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
    15421 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
    15481 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
    15541 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
    15601 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
    15661 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
    15721 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
    15781 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
    15841 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atgtcttaat
    15901 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaaga
    15961 tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct
    16021 taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg
    16081 ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt gtttttgtat
    16141 gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct taacaaaaaa
    16201 ccacaatgcg agattaagcg gatatatttt tgtggatttt tcagtaagtt ttttacgtct
    16261 attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga tagttttagt
    16321 cagcgaccgc aatatgcaat cgttagctaa ctactggcga aaacacaaca ccgcaatatc
    16381 tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca gacaactatt
    16441 tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg aattcaccat
    16501 aatggggtat atggtttctg gttataatcc atatcagatt gctgaagtgc tggatatgga
    16561 tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa
    16621 cgaattattt attcgttcac attcggtcca acattgatac tcaacgacca gccagaatca
    16681 tactctgttc atacgggcat ctattgaaat gcccgtattt tataaataag cagtaacccc
    16741 aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat
    16801 atgaatttac aaacaatagt ggcataaatg ttaaccatgt taatttacgt aaagttttac
    16861 gttgcaacat taaagcctca tttcaatcat catgataaat ataaaattaa tatatattta
    16921 tgccgtaaat accaatatac ttagcaaact atgtgatctc catttcgatt gatttagtgt
    16981 ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg agcaccgaaa
    17041 attaatatcg gtacataacg ccgatcttaa cgtcgttctg acaacgccag ccagtcgatg
    17101 tttatcactt ctacttgaag cttttcctga tgtggttgca caacaagatt ttttcaccag
    17161 agtctgggaa gaagaaggta tgcgtgtgcc tactaacacg ttatatcaga acatatccat
    17221 tatcagacgc ggatttcgcg ctgttggtga tactacccac tcgctaattg caaccgtgcc
    17281 gagagaggat tcaa
//
LOCUS       DAJVYM010000085        16808 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVYM010000085 DAJVYM010000000
VERSION     DAJVYM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.085"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OQS60_002553"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OQS60_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS2388275.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OQS60_002554"
     CDS             2219..2767
                     /locus_tag="OQS60_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS2388276.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OQS60_002555"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OQS60_002555"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS2388277.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OQS60_002556"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OQS60_002556"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS2388278.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OQS60_002557"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OQS60_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS2388279.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQS60_002558"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQS60_002558"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS2388280.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OQS60_002559"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OQS60_002559"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS2388281.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OQS60_002560"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OQS60_002560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS2388282.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQS60_002561"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQS60_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS2388283.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OQS60_002562"
     CDS             complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OQS60_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS2388284.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OQS60_002563"
                     /pseudo
     CDS             complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OQS60_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OQS60_002564"
     CDS             complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OQS60_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS2388285.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16250..>16808)
                     /gene="ssuE"
                     /locus_tag="OQS60_002565"
     CDS             complement(16250..>16808)
                     /gene="ssuE"
                     /locus_tag="OQS60_002565"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS2388286.1"
                     /translation="AGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAPEDLLYA
                     RFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATG
                     GTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTRLDTALE
                     TFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4187 a         4380 c         4296 g         3945 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcta gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccagatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctcca cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagtga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcga aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggaggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 caatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    13201 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    13501 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    13681 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    13741 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    13861 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    13981 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    14041 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    14101 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    14161 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    14221 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    14281 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    14341 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    14401 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    14461 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    14521 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    14581 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    14641 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    14701 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    14761 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    14821 gctccccgca catgaatatg tttaccgttg aaatcgacgc tttcccccag caataagcgc
    14881 cccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca
    14941 cgccgtcgcc tgccaactct tgtggatcgc tgcctgtgac cagattaaac aacgcacgac
    15001 catttgagag acggtcaagc gtggcggcct ggcgagcggc aacggtaggt gaggttacgc
    15061 tgggacgcag ggcgacaaga aacttcagcc gctgcgtcac cgggatcatc gacgccgcca
    15121 ccagccacgc atcttcacac gatcgccccg ttgggatcag cacaccggta tagcccagac
    15181 gatccgccgc ttgcgcaatt tgttgcagat agccgtgatc aaccgggcgt gaaccttctt
    15241 ccgttccaag ataatgcccg tcaccatggg tcggtaaaaa ccagaacata ttcagactca
    15301 taattgtttt ccttccaggt gagtgggctg ccagatgcgc tggcgaatat caactttttt
    15361 cgacaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc
    15421 agcgctcacc ggtttgatgg tggtgggagg acgatgatcc agatacgagg caatcaccgg
    15481 tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgctcgcgct ggctgcgggt
    15541 taacgcatcg gcctcactaa aggttgccag tacgccctga ataaaagcgc cgtttttttc
    15601 tgcatagggg cgagctgcca gataaaacga tccagtttga ttgagatcgg tgccgtcttt
    15661 cagcacccgc acgccgccct gtaataacgc agcggagtag tagggatccc agatagccca
    15721 ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatca gctggcgtca gataagtggg
    15781 ctggatgtca gtaaacttaa gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga
    15841 actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg ttttgatcgg
    15901 gctgttttct gccaccagaa tcacttcggc tttgggcttc ggtggctcga cgcccacata
    15961 tatcaaatca gccccggcag cctgggcaaa gattggcgga atatccccgg tactgccgag
    16021 atcaatactg ccaacgttta acgcttccaa catttgcgga cccgcgggga actccaccca
    16081 ggagattttt gattgcggat agcgtttttc cagtaactgg tggctttttg ccaataccat
    16141 accaatactg cctttctgat agcctatacg taacgcttca ggcgaggatt ctgcagcaac
    16201 cgcaaaagta gagacgctaa gcaatcccgc cagcgccagt ttaatgatgt tacgcatgga
    16261 cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc
    16321 agaaagtttc tagcgcggta tcaagacggg tttgcagatt tggcgtgaac tggggtttgt
    16381 gatggtaatc aataacttgt gagtcatcgg caaacacgcc gtgcaggagc tcctgggctt
    16441 tcatcgcact taaaactggc ttaagggcat aatcgaccgc cagcagatga gccaccgtac
    16501 cgcccgtcgc cagcggtagc accactttgc cttgcaaagc gcgctctggc agcaggtcga
    16561 gcagggtttt cagcgcgccg gaataggcgg ctttatacac aggtgtagcg actatcagtc
    16621 catcagcctg ttgcagctgt tcggtgaagg tcttgagtgc cggactatcg aaacgagcgt
    16681 aaagcagatc ttccggggcg aagttttgca gattccagtg ataaacctct acatccaggc
    16741 catttagttt ttcccgcgca tattccagca aggagctgga gcgagaagga aagcgaggac
    16801 tacccgcc
//
LOCUS       DAJVYM010000086        16620 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVYM010000086 DAJVYM010000000
VERSION     DAJVYM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.086"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(93..2534)
                     /gene="rnr"
                     /locus_tag="OQS60_002566"
     CDS             complement(93..2534)
                     /gene="rnr"
                     /locus_tag="OQS60_002566"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS2388287.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2573..2998)
                     /gene="nsrR"
                     /locus_tag="OQS60_002567"
     CDS             complement(2573..2998)
                     /gene="nsrR"
                     /locus_tag="OQS60_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS2388288.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3203..4501)
                     /gene="purA"
                     /locus_tag="OQS60_002568"
     CDS             complement(3203..4501)
                     /gene="purA"
                     /locus_tag="OQS60_002568"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS2388289.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4605..4802)
                     /locus_tag="OQS60_002569"
     CDS             complement(4605..4802)
                     /locus_tag="OQS60_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS2388290.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4884..5888)
                     /gene="hflC"
                     /locus_tag="OQS60_002570"
     CDS             complement(4884..5888)
                     /gene="hflC"
                     /locus_tag="OQS60_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS2388291.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5891..7150)
                     /gene="hflK"
                     /locus_tag="OQS60_002571"
     CDS             complement(5891..7150)
                     /gene="hflK"
                     /locus_tag="OQS60_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS2388292.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7236..8516)
                     /gene="hflX"
                     /locus_tag="OQS60_002572"
     CDS             complement(7236..8516)
                     /gene="hflX"
                     /locus_tag="OQS60_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS2388293.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8593..8901)
                     /gene="hfq"
                     /locus_tag="OQS60_002573"
     CDS             complement(8593..8901)
                     /gene="hfq"
                     /locus_tag="OQS60_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS2388294.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8987..9937)
                     /gene="miaA"
                     /locus_tag="OQS60_002574"
     CDS             complement(8987..9937)
                     /gene="miaA"
                     /locus_tag="OQS60_002574"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS2388295.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9930..11777)
                     /gene="mutL"
                     /locus_tag="OQS60_002575"
     CDS             complement(9930..11777)
                     /gene="mutL"
                     /locus_tag="OQS60_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS2388296.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11787..13124)
                     /gene="amiB"
                     /locus_tag="OQS60_002576"
     CDS             complement(11787..13124)
                     /gene="amiB"
                     /locus_tag="OQS60_002576"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS2388297.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13143..13604)
                     /gene="tsaE"
                     /locus_tag="OQS60_002577"
     CDS             complement(13143..13604)
                     /gene="tsaE"
                     /locus_tag="OQS60_002577"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS2388298.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13576..15123)
                     /gene="nnr"
                     /locus_tag="OQS60_002578"
     CDS             complement(13576..15123)
                     /gene="nnr"
                     /locus_tag="OQS60_002578"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS2388299.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15122..16261
                     /gene="queG"
                     /locus_tag="OQS60_002579"
     CDS             15122..16261
                     /gene="queG"
                     /locus_tag="OQS60_002579"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS2388300.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16244..16297)
                     /locus_tag="OQS60_002580"
     CDS             complement(16244..16297)
                     /locus_tag="OQS60_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388301.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(16531..16606)
                     /locus_tag="OQS60_002581"
     tRNA            complement(16531..16606)
                     /locus_tag="OQS60_002581"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16571..16573),aa:Gly,seq:gcc)
BASE COUNT         3762 a         4752 c         4179 g         3927 t
ORIGIN      
        1 tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaagcgcat tttgtcagca
       61 atctaaccct cttcttttaa agagggtatt gatcactctg ccactttttt cttcgccgca
      121 cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt
      181 ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct caccgcggaa ggcgctgtct
      241 ggctcaaagt ttaccttttt accgacctga cgacgcttgc cgcctttttt gcctgcatcg
      301 ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag
      361 atcagactaa agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg
      421 cgatcgccca ggcgataagt ctggccgctg gattccccca tcaggcgttg ccctacctgg
      481 tcaaagcgat agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag
      541 tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc tttaaagacg
      601 ttacctacct ggtcgagcat gaagtcacac ttcagccagt cagcgacatc gcgcgttgct
      661 tcgtcggcac gacgttccgc catcgaacag tgctgaccca gttgcaacat ctcttccatc
      721 gaataatggt agccgccggt ttcagtggtg ttgccctgat gcccctgctc tttcgccagc
      781 agatacttaa tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag
      841 tgcgcatagg actgcaatgc caggccaaag tgaccacggt tttctggatc gtaaatcgcc
      901 tgtttcatcg agcgcagcag catggtttgc agcatttctg catcaggacg gtcggcaacc
      961 gactccagca gttccgcgta gtcacgcggt tccggcttgt taccacccgg cagctccagc
     1021 cccagctccg ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga
     1081 atacggaaca gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc
     1141 agaatcatgc actcttcaat taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt
     1201 tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca
     1261 ccgcgttctt cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc
     1321 ttaaccagcg ggacgtactg ctcgcgcaga tcctgatcgc cctgcagaat atgccagact
     1381 ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt cgtagaattt gtagcccgtc
     1441 aggcggcctt tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg
     1501 ttgagcgaac acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg
     1561 aagtacaccg acgtgccacg gttacgcgct tctctgtcca gcggcgttgg cggacgcaca
     1621 tagtagctga cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg ttttttctcg
     1681 cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa
     1741 tcgcgcaaat cgacacggcc cgcttttgct tcttccggca cttcttcttt cagccccgca
     1801 acctgttgct caacagcctg cggccagata tacggaattt catgggtacg cagagcgata
     1861 tcaaccgcca tgccggtgcc catattgtcg cccagcactt cgacgatttt acccaccgct
     1921 ttggtgcggc gagtcggacg ctgagtcagt tcgactacga ccacaaagcc catccgcgcg
     1981 cccatgatct gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc
     2041 acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa tctggctggt ttttggcacc
     2101 agtacgcgga caatacgcgc ttcacgacga cctttacggt cagcacccag cggctgcgcc
     2161 agcacctgat cgccatgaat acaggttttc atctgctcgc tggagagata caaatcatct
     2221 ttacgccctt caacccgcag aaagccgtag ccatcacggt ggccaataac ggtacctttc
     2281 accaggtcga ggcgttccgg cagcgcatag cactgacgac gagtgaagac cagttgacca
     2341 tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg
     2401 tgcagttcta ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg
     2461 aggataaatt cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa
     2521 ggatcttgtg acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca
     2581 ccagcaataa tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat
     2641 ccagttccgt aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag
     2701 gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca
     2761 ccacatcacc aatacgtatc gcactcgccg gtttacccag gcgaatgccg ccattttttc
     2821 cacgaacagc agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat
     2881 tacgggagac gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca
     2941 atgacgccat gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat
     3001 caacctcaaa agggaaatcg ggaaaaaata catttatatt gatgataaac cagccacaag
     3061 ctgtgtcgct aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc
     3121 ggatagcgta atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa
     3181 atatctgcca ggcgtaccag aattacgcgt cgaacggatc gcgcagaatc atggtttcag
     3241 tacgatccgg accggtagag atgatatcga tcggcacacc ggtcagctct tcaatacgct
     3301 tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc tttcacgccg aaagtggatt
     3361 cagaccagcc cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca
     3421 gcggagtggt agccacttcg cgaccatccg gcatacggta agccacgcag agtttaacct
     3481 ctttcaggcc atccagaacg tccagcttgg tcaggcaaaa gccagacagg gagttcagct
     3541 gtaccgcacg acgaacggca acggtgtcca gccagccggt acgacgacga cgacccgtag
     3601 ttgcgccgaa ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg
     3661 tcgggaacgg acctgcacct acacgagttg agtaagcttt gaggataccc agaacataat
     3721 caacataacg cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag
     3781 aagttacgta cggataagta ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa
     3841 acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca
     3901 tagaagtcag gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg
     3961 cttcagcttt gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt
     4021 tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgctactt
     4081 tatcttcata agcaggcccg ataccacgac cggtggtgcc gatcgctttc gcgccacgcg
     4141 ctttctcacg cgcgttatcc agcgcaacgt gataatcaag gatcagcgga catgcttcag
     4201 acagcagcag acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca
     4261 tcagcgcggc cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga
     4321 gaatacctga tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc
     4381 ctgcgttgtg accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga
     4441 cgatcttacc tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca
     4501 tttttcaaaa tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag
     4561 cactttttgc actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc
     4621 ctcaacatgt agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa
     4681 atattatcgg gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc
     4741 atcggcccta aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc
     4801 attcattatc cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat
     4861 tgacctgtac cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg
     4921 aagaaatcgc tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca
     4981 taagcacgca ggctacggat gaatgcgtag aagtccggat ctttactgaa tgcatcagca
     5041 aacagtttgg ctgcttcggc atcgccttca ccacgcatga tgcggccctg acgctcagct
     5101 tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc
     5161 tgaccttgtg aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag
     5221 atcgcttcag acacttcggt cggcaggttg atctgcttga tacgcacatc gacaacttca
     5281 atacccagcg ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc
     5341 gttacgcgct ctgccgcttc ggcaatggcg ttatctgccg ccggggtagt aacttcatct
     5401 tctgtacccg cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg
     5461 gaatcggtga cgatgtcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag
     5521 aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag
     5581 taacggctga aatcgctgat gcgccatttg atgtaagaat cgacgatcag gtctttcttc
     5641 tctttggtca caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt
     5701 ttcaccgttt caatgaacgg tatcttgaaa tgcagacccg gctcataaac cagaggtttg
     5761 ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg
     5821 acgacaaaga cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac
     5881 ttacgcatcg ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt
     5941 ggtccataat atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg
     6001 aagaggctgg cggcagacgc agcagattgc tggcaccgtt atcgctcttc gccgcagggg
     6061 cgttaccgcc tttcagcatc tggtctaacg gcagaaccat caggttgcca cctttatcgt
     6121 taaccagcac tttgcgggtg ttacccaaca ctttttccat cgtctcgata tacagacgct
     6181 cgcgagtaat ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt
     6241 caccctgagc ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt
     6301 gcgcctgacc gttcgcacgc ggctgaactt cgttggtata cgcttctgct tcacgaatgt
     6361 attgctgttc gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg
     6421 gcggacgagc tgcctggaag ttgacgtcca gcagagtgat acccatgtca tacggacgga
     6481 tagtctcttc cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa
     6541 tgcggtccat ggtgtattta ccgataactc cacgcagggc gctgtcggta gcctgacgca
     6601 ggctgtcatc cgggctggtc acgctataca gatatttttc cggattggtg acgcggtact
     6661 gcacgttcat ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg
     6721 ccagttcacg cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc
     6781 agttcagacc cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccgcgtt
     6841 cggcttcttt aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg
     6901 cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc
     6961 cagatccggt gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga
     7021 tatcatctaa atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt
     7081 tgccgccagg tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat
     7141 tccacgccat gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc
     7201 agaggatatg actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc
     7261 ggttcttgtt tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg
     7321 ctgccgtcct cctccatcca ctctttttct attgcctgaa gctgataaaa acgacttctc
     7381 agacgtcctt cctgcggcgg caaacgcaat gtatgctgcg ccacctcgcc ggaaagccgc
     7441 tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg tctgtgcgga aagccagaca
     7501 cggatcggtt tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg
     7561 atcttgttca tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta
     7621 ttcaccgctt cgatgttttc ttgtacacgt acatccgccg catcaatgac gtgcagcagt
     7681 aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg ctgccaccag atcgtgcggc
     7741 aggtggcgaa taaaccctac ggtatctgca agtacggttt caccgacatc cgcgacgtca
     7801 atacgccgca acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct
     7861 tcggtgatgc gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca
     7921 gtaggaacgt cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt
     7981 tctaggcgcg actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc
     8041 tgggtttcac ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag
     8101 ccacgcacca ggcgcgtagc cagatggcgc agctgcgcta gctcaacctg caacttaccc
     8161 tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca
     8221 cgacactcgc acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa
     8281 agaacgaccg aagcacccgt cgctttgaca gcttccgcaa tttcaactgc tttaccttca
     8341 cctacaaaat actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg
     8401 acaccggcgg aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc
     8461 gtaaaataga tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt
     8521 ataaccctct aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaaa
     8581 cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct
     8641 gctaccatga tggtagttac tgctggtacc gccaccggcg ttgttactgt gatgagaaac
     8701 cgggcgagac gggacaacag tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt
     8761 tttcaacagg atcacgaact gatcaaaaga ctcgatttgc ccttgcagct taataccatt
     8821 caccaaataa atagaaactg gaacacgttc ccgacgcagt gcgttcagga acggatcttg
     8881 taaagattgc cccttagcca ttctctcttt tccttatatg cttatttgta ctttgaacct
     8941 ttcgattctg aaaaaattgc gcacgatacg tctcaattgt acacattcag cctgcgatag
     9001 caccaacaac ctgtaatact tcgtcacgcg cctgttctgg tttttcactg tcaagccagt
     9061 gaaccccttc ccaaccacgc agccaggtta tctgccgctt cgccaactgt ctcgtggcgc
     9121 aaacacctcg ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct
     9181 ggcgataacc cacgcaacga atggaaggca agtccgtatg caaatctcct cgggcaaaaa
     9241 gcgcccggac ttctgcttca aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc
     9301 gttgatggag cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta
     9361 gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt
     9421 ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc gcaaccggat
     9481 ctacctcctg aagttgacga tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa
     9541 ttctggcccg cacttccggg tctgccgacg gtagcggcga caacccttcc agcaatgcct
     9601 tgaaatacaa catcgtaccg cccactaaca gtgggatccg ccccgccgcg gtgatatcgg
     9661 ccatttccgc cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc gacggatcgc
     9721 gaatatccag caatcggtgc ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc
     9781 cgatatccat ccctttgtaa ataagggcag aatcaacgct tatcaactct actggtaaaa
     9841 ttttacgcag ctcaatggct aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa
     9901 tcgccttagg caggctcgcc ttactgatat cactcatctt tcagggcttt tatcgccgga
     9961 tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt tcacaagttg cggacataac
    10021 cgttccacgt ccgccagcag ggttatggcc tgtgccattg accactgcgc atgttcgctc
    10081 atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc
    10141 aggtagccta tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc
    10201 acggccctga tggtcacatg ctgtgcatct gactggaaat caatacccaa ttccgccagg
    10261 gcagactgcg ctttttctaa tgccgatttt tcttcgccag aaacttttag ccgcaacgga
    10321 ataagcagcg gctgagcgca aactggcgct tcacccggcg tcagttgtac ctgacgcagc
    10381 caacgttctg ccactggcaa ggctaaaagt gaaatgttgc cgtcgcgctc cagcaacgca
    10441 cagtcggaat ggacgatagt cagtacccga ccaaaactct gactgttcgc cgcaagtgca
    10501 ggttcctgcg gttccggcgc ttttaatttt tgcatcggcg cgggcgtttg caaaagctgg
    10561 cgatacactt caccttgctg tttctggtag cctggctgcg cattcggcca gggggcagcc
    10621 ggacgactgc ctgatgctgg cgcaggagtg tagcgcggag ctaccggctc acgagctgcc
    10681 ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt ccggaatgga acgcggtgca
    10741 ggttggggtt catcgtccag cggtagcggc gtttccagtt gctgttgtag cacgctcagc
    10801 acgccctgat agataaaatc atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg
    10861 gcggggtgca cgttgacgtc cacctgatgc ggatcgatct ccagatacag cacaaatgcc
    10921 ggttgctgat cggcccccag tttgtcttcg caggcctggc ggatcgcgtg attgatcagg
    10981 cgatcgcgca tcatgcgacc gttcacgtag caatactgaa tttctgccag tgcgggcgtg
    11041 gtgtgatttg gatcggccac ccagccgcgt agcgtgagat cgccgtgttg ccattcaatc
    11101 gccagcgctt gttcaagaaa agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc
    11161 ccgccttccg gcactgcgcg gtactgacgc acaattttac cgttatgcga caggttgatc
    11221 gtgacgtcga aacgcgccag cgcaatgcgg cggatgatct catcaatgtg gttaaattcg
    11281 gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag atccagcacc
    11341 tccagcgtcg tccccacagg atgcgccgcc ggttttaccg tcacgttcat atcgcgccct
    11401 tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg
    11461 gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg
    11521 agatcgtcca gagaggcgat tttactggtg gcatgacgag ccagcgccag cgccagctca
    11581 tcttttttga taccgcagcc gttatcacga atgcggataa gtttcgcccc accgcgttcg
    11641 atatcaatat cgatacgcgt cgcacctgca tcgaggctgt tttccaccag ttctttgact
    11701 accgacgcag gtcgctcgac cacctcacct gcggcaatct ggttcgccag ttgtggcggt
    11761 aagacctgaa ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc
    11821 gtactggcag tttgtgccgt tgccccctgc ggcgcagatt gcatcggatg cgcaaggaaa
    11881 taattgcgca ggcctttgta aatggcttct gccagctgtt gttggtaatc gtcgctcgcc
    11941 agcaaacgtt cttcgctgtt gttgctgata aaaccggttt cgaccagtac tgacgggata
    12001 tccggcgaac gcagaacgcc gagactcgcg tgttctggtc ggcgtttatg tatttcgcca
    12061 atgcgttgca actgactgat catactggtc gctacatcat accctacccg ctgggaatga
    12121 ccgaactgta aatccagcac cgcctggctt aaataggggt cagactgact gttcgccagc
    12181 acatcgcccg ccccacccag cagctccgac tgtttctcgt gctgctccag ccagctggcc
    12241 atttcgctgt tggcgcgacg gttagagagc acccatacgg aagcgccagt cgcactgcgg
    12301 ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg cgttttgctt acgtgccaca
    12361 tcgctgcgcc ccatcaccga gataaagtaa tccccgtcac gggttaaaac gcctttaaac
    12421 atcggatcgt cattgagcaa agtgcgcaat ttacgcgcga tggcgatggt gacatttttc
    12481 tcccgcgtac caccggggcc gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata
    12541 gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta
    12601 ttactgctga taacacccgt agtgcggtta ctttccgttt taaacggatt gcgcgccggt
    12661 tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggt
    12721 ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga cgacagtgta attgctgcca
    12781 ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa gatcgaccac cagccgtagc
    12841 gtttgtgcat ctttaggcgt tccagagcga atcgccttca ccagattatt gccgctgaac
    12901 aacaacggca gtccctgaat cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg
    12961 ctttgatggc taaacgcata atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt
    13021 tgattaccgt tagaaacctg aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc
    13081 agcagcagcg tcgctaccaa ccaatttctg atgcgataca tcatcccgcc acctttcaaa
    13141 ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc actcacgcgc
    13201 gcctcacggc cttgtgcctg ataatcaatg tgtatttcga catccgggtc aggaagaaca
    13261 cctgtacctt gttgtggcca ctccaccagg cagatggcat cgttggcaaa ataatcgcgg
    13321 atccccataa actccagctc ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc
    13381 attaaattgt cgagcgtata gggttcgacc agcgtataag tggggctttt gacattaccc
    13441 tgatgaccca gagcttgtaa aaaaccccgg ctaaaggtgg ttttacctgc gcctaaatcg
    13501 ccatacagat agattacggt tgcgccatcg caggctttcg ctacccgctc gcccaggtct
    13561 aatgttgcct gctcatcagg gagcggaatt actcgattca tcatggtttt tatcagtcac
    13621 ttccgggtta acaatacgct gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt
    13681 tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc gcgacacagc ccgcacaggc
    13741 ggcatcatac ggcgacagtt tttgcccaag caatgcgcca ataataccag agagcacatc
    13801 gcccatgccg ccgctcgcca tgcctgcatt tccggcatca ataatgccta aagcgtcagg
    13861 atgggcggcg accacggttc cggcaccttt cagcaccgct acgccgccat aacgttgtac
    13921 cagacgtttg gcgcaatgta agcggtcact ttcaatttca gcgacggaac agcctaacaa
    13981 ccgtgcggcc tcgccaggat gcggcgtgat cacgcgattg tgacgcttat cgggattgat
    14041 tgccagcagg ttcaatgcat cggcatccca caacatcggt ttgcgaaaat tctcaacttt
    14101 ttgcagtgct tttttccccc actcttgctg gcccagaccg ggaccaatca ccaccacatc
    14161 ggcccattcc aggctttcgg taagagagtc catcgtcagt tcatgcacca tcaattccgg
    14221 tcgtgcagtc agcagcggcg caatgttttc actgcgggtc agtactcgga ctaaaccagc
    14281 accagcacgc agcgccgctt cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc
    14341 accgataatc accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac gcggtttcag
    14401 ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca
    14461 gctatccagc cccagtgagt caaaatgcag ttgtcctgta acatcccgcg cttttccagt
    14521 aagcaagcct ggtttcagcg caataaaagt gatggtgtga tcggcgttga tcactgcgcc
    14581 tggcgtagcg ccggtttcag ccagcaggcc ggaagggata tcaaccgcca caatcggcgc
    14641 aggatgggaa ttagcgtggt cgattaactg gctaatggat tcgcggggcg cttgctgcaa
    14701 gccggtaccg agcagcgcat caacaatcag atctaccgat tcgggccaga caatattcga
    14761 agcatggatc tcgccacccg cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg
    14821 caacggtttg tcgctctcct gggccaacaa cgtgacctca atgccgaccg ctttggccag
    14881 tcgcgcgacc acgtagccat cgccgccgtt attaccatga ccgcacagca ccagccagtg
    14941 gcgggcgtca ggatacgccg aacgacacac ctggaatgcg gcctcgccag cgcgaagcat
    15001 cagctcatag agtgtgagcc ccagcacatc tgccgcctcg cgttctccgc ggcggatatc
    15061 gtcggcgtac cagacggtgt gtggtatact tacggggttt ttcttcattg tatggtccgt
    15121 catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa attaaacagt gggggctgga
    15181 actgggcttt cagcaggtag gtattaccga taccgatctc agcgagtccg agcccaaact
    15241 gcaagcatgg ctggacaaac aataccacgg cgaaatggac tggatggcac gtcacggtat
    15301 gctgcgcgct cgcccccatg agttattacc aggtacgctg cgtgtaatca gcgtgcggat
    15361 gaattacctt cctgctaacg ccgcatttgc cagcacgctg aaaaacccca aactcggcta
    15421 tgttagccgt tatgcgctgg gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa
    15481 gctgggcgag atgattcagc aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc
    15541 tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg ctcggctgga caggtaagca
    15601 ctcacttatc ctcaatcgcg aggccggttc tttcttcttt ttaggcgaat tgctggtcga
    15661 tattccgctg cccgtggatc aaccagtcga ggaaggatgc ggtaaatgca tagcctgtat
    15721 gacgatttgc ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc gccgctgtat
    15781 ctcttatctc accatcgaac ttgaaggggc gatcccggaa gagttgcgac cgttaatggg
    15841 aaaccgtatt tacggttgcg atgactgcca gcttatctgc ccgtggaatc gctattcgca
    15901 actcactaca gaagacgatt tcagcccgcg taagccgcta cacgcacagg aactcattga
    15961 gttatttgcc tggagcgaag agaagttttt aaaagtcacg gaaggatcgg cgattcgccg
    16021 tataggtcac ctgcgctggc tgcgtaatat cgccgtggca ttaggcaatg cgccctggga
    16081 tgaaacgatt ctggcggcgc tggagagtcg caaaggtgag cacccacttc ttgatgagca
    16141 catagcgtgg gcgattgcgc agcaaatcga gagacgaaat gcgtgcatag tcgaagtgca
    16201 attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg
    16261 attcatccac agcttgtgaa taaaaataaa aacgcattgc aattcaagac gcataaaatc
    16321 gtcaagtgat cgactcgaca aaacggaatc ttgattttta acttatttat caataggtta
    16381 aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggatgtg
    16441 gataactctg tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt
    16501 cgctgtggat attttattga gagaagaatt tggagcggga aacgagactc gaactcgcga
    16561 ccccgacctt ggcaaggtcg tgctctacca actgagctat tcccgcttgg gtggtctgtg
//
LOCUS       DAJVYM010000087        16295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVYM010000087 DAJVYM010000000
VERSION     DAJVYM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.088"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..577
                     /locus_tag="OQS60_002582"
     CDS             <1..577
                     /locus_tag="OQS60_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS2388302.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            580..1743
                     /locus_tag="OQS60_002583"
     CDS             580..1743
                     /locus_tag="OQS60_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS2388303.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1827..3452
                     /locus_tag="OQS60_002584"
     CDS             1827..3452
                     /locus_tag="OQS60_002584"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS2388304.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="OQS60_002585"
     CDS             complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="OQS60_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS2388305.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="OQS60_002586"
     CDS             complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="OQS60_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS2388306.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4504..5253
                     /gene="yjfP"
                     /locus_tag="OQS60_002587"
     CDS             4504..5253
                     /gene="yjfP"
                     /locus_tag="OQS60_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS2388307.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="OQS60_002588"
     CDS             complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="OQS60_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS2388308.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="OQS60_002589"
     CDS             complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="OQS60_002589"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS2388309.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7532..8930
                     /gene="ulaA"
                     /locus_tag="OQS60_002590"
                     /pseudo
     CDS             7532..8930
                     /gene="ulaA"
                     /locus_tag="OQS60_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8946..9251
                     /gene="ulaB"
                     /locus_tag="OQS60_002591"
     CDS             8946..9251
                     /gene="ulaB"
                     /locus_tag="OQS60_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS2388310.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9261..9725
                     /gene="ulaC"
                     /locus_tag="OQS60_002592"
     CDS             9261..9725
                     /gene="ulaC"
                     /locus_tag="OQS60_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS2388311.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9739..10389
                     /gene="ulaD"
                     /locus_tag="OQS60_002593"
     CDS             9739..10389
                     /gene="ulaD"
                     /locus_tag="OQS60_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS2388312.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10399..11253
                     /gene="ulaE"
                     /locus_tag="OQS60_002594"
     CDS             10399..11253
                     /gene="ulaE"
                     /locus_tag="OQS60_002594"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS2388313.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11253..11939
                     /gene="ulaF"
                     /locus_tag="OQS60_002595"
     CDS             11253..11939
                     /gene="ulaF"
                     /locus_tag="OQS60_002595"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS2388314.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12036..12587
                     /locus_tag="OQS60_002596"
     CDS             12036..12587
                     /locus_tag="OQS60_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS2388315.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12662..12977)
                     /locus_tag="OQS60_002597"
                     /pseudo
     CDS             complement(12662..12977)
                     /locus_tag="OQS60_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13304..13699
                     /gene="rpsF"
                     /locus_tag="OQS60_002598"
     CDS             13304..13699
                     /gene="rpsF"
                     /locus_tag="OQS60_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS2388316.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13706..14020
                     /gene="priB"
                     /locus_tag="OQS60_002599"
     CDS             13706..14020
                     /gene="priB"
                     /locus_tag="OQS60_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS2388317.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14025..14252
                     /gene="rpsR"
                     /locus_tag="OQS60_002600"
     CDS             14025..14252
                     /gene="rpsR"
                     /locus_tag="OQS60_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS2388318.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14294..14743
                     /gene="rplI"
                     /locus_tag="OQS60_002601"
     CDS             14294..14743
                     /gene="rplI"
                     /locus_tag="OQS60_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS2388319.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14855..15826)
                     /locus_tag="OQS60_002602"
     CDS             complement(14855..15826)
                     /locus_tag="OQS60_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS2388320.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15879..>16295)
                     /locus_tag="OQS60_002603"
     CDS             complement(15879..>16295)
                     /locus_tag="OQS60_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS2388321.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4154 t
ORIGIN      
        1 tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac
       61 gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtagcga
      121 tgtcgataac gacggcgacg gaacgtttta cgcgacagtg caggattgta ttgatgacgg
      181 taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaaacggcat tttatgccga
      241 tgttccgaag aatatgactc agcagaactg tcagtctaag tacgaaaatt cctactacga
      301 caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg
      361 taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcccgccc
      421 cgtctggcgc aacacttccg gtgattactc ctggcgctcc ggttctggca aaaaagagtc
      481 ttactcttca ggcggcttta ccaccaaaaa agcgtcaacc gtttcgcgcg gcggctatgg
      541 tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct
      601 gtgcggcggg atctggacca aattgccgct gacaacggtt tcgactttca tatcatcgac
      661 aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag
      721 cagatcgaaa aaccaactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg
      781 gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc
      841 gccgaaagct ggcacgccag cgatccttcg ctgtatggcc ggatggattt tgcctggtgt
      901 ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacttc gttgtatgag
      961 tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat tattccgcgt
     1021 gatgccgatc agtacaatgc cattcaggaa cgcctgattt cacgctttag cgagctttat
     1081 agccgggagc cgttttattt ctgctgctgt caggacaccg atgaagacag gactaccgtg
     1141 ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa
     1201 gatctcggtt tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt
     1261 gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgtgc
     1321 aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta
     1381 atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggtttgag
     1441 ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc
     1501 gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgatggt
     1561 gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat
     1621 agctacacac tcatcgatag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt
     1681 gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga
     1741 taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtggcca tactaatggt
     1801 gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac
     1861 ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa
     1921 cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag cagttaggaa
     1981 cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct
     2041 acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg cacctgctga
     2101 tgcaggtgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg
     2161 gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt
     2221 tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg ttacccacgc
     2281 cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac ttattgccag
     2341 gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggtggttccg
     2401 atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat cggctggtgg
     2461 ggcataaatg gtttttctcg gttccgcaaa gcgatgcgca tctggtgctg gcgcagacca
     2521 cgggcggtct gtcctgtttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga
     2581 ttcgcctcga acggctgaaa gataagctgg gtaatcgctc taacgccagt tgtgaagtgg
     2641 agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc
     2701 tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc
     2761 gtgcgttttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
     2821 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
     2881 cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg cgccgatgcc aaagaagccc
     2941 tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt
     3001 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
     3061 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt
     3121 gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag
     3181 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
     3241 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
     3301 tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc
     3361 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
     3421 tgctgcgggc gacgggggga gtgtgtttgt aaacgtatac gactgatgcg acgctggttt
     3481 cgattaactc aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc
     3541 ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt
     3601 tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat
     3661 cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg
     3721 agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact
     3781 gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt
     3841 tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata
     3901 aacgcccagg cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag
     3961 caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa
     4021 taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt
     4081 gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc
     4141 gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga
     4201 tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac
     4261 atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc
     4321 atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac
     4381 gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat
     4441 accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga
     4501 cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct
     4561 gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc
     4621 agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg
     4681 ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa
     4741 ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc
     4801 gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg
     4861 acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga
     4921 tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca
     4981 gcacagcaga atgaattcaa taacattatc gcgccgctgg cagagtggga agcgacaaac
     5041 cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt
     5101 gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa
     5161 ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat
     5221 gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct
     5281 tccagttgtt gcaggatttc cgggttagca tttttgccgg tgataagcat atcgatttga
     5341 tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc
     5401 accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt
     5461 ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag
     5521 agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca
     5581 cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa
     5641 ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag
     5701 ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata
     5761 cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc
     5821 tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgcg ttcgtcaagt
     5881 ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca
     5941 acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca
     6001 gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat agctaggaaa
     6061 tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga
     6121 atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt
     6181 ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa
     6241 acggcttgaa gccatacttc agacgatctt ttttcatctc ccacagcacg cggatctctt
     6301 gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat
     6361 tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg
     6421 ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat
     6481 aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca
     6541 ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg
     6601 caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt
     6661 tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc
     6721 caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct
     6781 gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta
     6841 ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg
     6901 gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt
     6961 taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt
     7021 ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga
     7081 cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga
     7141 tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt
     7201 caattcgaat caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc
     7261 aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat
     7321 ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg
     7381 tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta atcataaata
     7441 atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa
     7501 tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt
     7561 gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg
     7621 ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat
     7681 tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt
     7741 ggcgaaaatg tccgaagtct acggcattaa acggcgcaat ttccgatact tacgcgtcga
     7801 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
     7861 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
     7921 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
     7981 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
     8041 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
     8101 tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc
     8161 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
     8221 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
     8281 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc
     8341 tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt
     8401 ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg
     8461 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
     8521 ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
     8581 cgagcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
     8641 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
     8701 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga
     8761 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
     8821 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
     8881 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
     8941 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
     9001 tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct
     9061 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
     9121 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc
     9181 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg
     9241 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
     9301 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
     9361 tggcggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
     9421 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
     9481 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg
     9541 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
     9601 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
     9661 tacgtgcctg ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag
     9721 cttaagaagg aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact
     9781 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
     9841 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac
     9901 ccgcacaaaa tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt
     9961 atgtgcttcg aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc
    10021 gccaaaggtg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
    10081 ggttactgga cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt
    10141 tatcaccgca gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact
    10201 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    10261 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
    10321 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
    10381 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    10441 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
    10501 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
    10561 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
    10621 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
    10681 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
    10741 cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    10801 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg
    10861 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    10921 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    10981 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11041 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11101 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11161 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc
    11221 atggccaaag caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg
    11281 aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg
    11341 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
    11401 tgaaagcaga cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc
    11461 gcccgtcttc cgacaccgcg acgtatctcg aactctaccg tcgttacccg tcgcttggcg
    11521 gcattgtcca tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc
    11581 cggcgttagg cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat
    11641 taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa
    11701 cgctgggtga cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt
    11761 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg
    11821 cgaaaatggc gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc
    11881 tcatgaataa acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag
    11941 cagtgcgccc cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct
    12001 tcagttaaaa aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca
    12061 gaacggatcc gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa
    12121 ttatttcagc atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg
    12181 gtggtcgcga tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc
    12241 caacccaaac tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata
    12301 tcatcttctc actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag
    12361 tgaaattatg gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat
    12421 tttgcccggc gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct
    12481 ctgaaatcct ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac
    12541 gtaatcgcat tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa
    12601 aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt
    12661 gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca
    12721 gaattacgtg gtagtacgtt actccccggt tcccgcatca gaattacgtg gtagtacgtt
    12781 gcgccctgcg catctgtctc ttcatttaat gcctgacgcg cttcgctttc agtggcgaaa
    12841 ttatgattga tataaatcac gcctaagctt tgcacatcat ccatgtttct ggcctggtgg
    12901 ttattaattt caatggctgc ccatgtattt gcacttagca aaagcacagc cagaagggct
    12961 aaaactcgac tgaacataga tacctcctcg acggctgact ttgtgtgctc tcctctgtga
    13021 tgatcttctg atttaatttt aatcaatgat aaagaagttg atggtgacca tttctgatgc
    13081 agttgttcaa aaaaacacca tgatgaagtg tgatgaactc caaatcagcg gtttagaggt
    13141 taattgcgaa aggggagatt tatttcggct ctgcccttga gtttagcggg gcatacaagt
    13201 actataacgc gtcatttttc agccgacctt taacacgttc cttgcctccc cgggattcgg
    13261 ctgacccaga caggaggctg aataatccgt aaggagcaat tcgatgcgtc attacgaaat
    13321 cgtttttatg gtccatcctg accagagcga acaggttccg ggcatgatcg agcgctacac
    13381 tgctgccatc actggtgcag aaggcaagat ccaccgtctg gaagactggg gccgccgtca
    13441 gctggcttac ccgatcaaca aactgcacaa agcacactac gttctgatga acgttgaagc
    13501 tccgcaggaa gtgatcgatg agctggaaac taccttccgc ttcaacgatg ccgttatccg
    13561 cagcatggtt atgcgtacca agcacgctgt taccgaagca tctccgatgg ttaaagcgaa
    13621 agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg atgctgaagc
    13681 tggggattct gaagagtaat ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg
    13741 caggactccc cttcgaaagg tcagtccatc aggaattcct cactgccagt tcgtgcttga
    13801 gcatcgttct gtgcaggagg aagccggttt tcaccggcag gcgtggtgcc aaatgcccgt
    13861 tattgttagc ggacacgaaa accaggccat tactcacagt ataacggtcg gcagtcgcat
    13921 aaccgttcag gggttcattt catgccacaa ggcaaagaac ggactgagca aaatggtttt
    13981 gcatgccgag cagattgaat tgatagattc tggagactag ccatatggca cgttatttcc
    14041 gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca agagatcgac tataaagata
    14101 tcgctacgct gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg
    14161 gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt
    14221 ccctgctgcc gtacactgat cgccatcagt aatcggtcac ggtccattaa tacgactttg
    14281 agaggataag gtaatgcaag ttattctgct tgataaagta gcaaacctgg gtagcctggg
    14341 tgatcaggta aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc
    14401 tgttccagct accaagaaaa acattgaatt cttcgaagca cgtcgcgctg aactggaagc
    14461 taaactggct gaagttctgg cagctgctaa tgctcgcgct gagaaaatca atgcactgga
    14521 aactgttacc atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac
    14581 tcgcgacatc gctgacgctg taactgcagc tggcgttgaa gtggctaaga gcgaagttcg
    14641 tctgccgaac ggcgttctgc gtaccactgg cgagcacgaa gtgagcttcc aggttcacag
    14701 cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga
    14761 cgtaaaaacg ccgaccagtg gtcggcgttt tgcttttgta gcgagcactt gcagatatcg
    14821 ataagggcca caataagcag ccctacgaag ataattactt agctgcctga atatcagcca
    14881 gcattggggc aagggctggg ttgtccttag cgaagtttgc gtacattggc tgtactttct
    14941 cgataaacgg agctttatct acctggtaga ctttcacgcc cattttatcc agttttgcaa
    15001 attcttgttt ctcttcctcg ctccacaagt ctttcatgta gctcattgat tctttagctg
    15061 cttttaatat aactgcctga ttttccgggc taagtttatc aaacgcagca ttgctcatca
    15121 gaaccacatc gggaaccata gtgtgttcgt caaggctgaa ggattttgcc acttcaccgt
    15181 gacgcatggt ggtcagcgcc atcacgctgt tttctgccat atcgaccaca ccctgctgta
    15241 aggctgtata caactcgccg taatccagtg gcgtcgggtt accgcccatg actttgatca
    15301 tttcaactgc gccagggctt ggctggacac ggactttcat gcctttcaga tcgtctggtt
    15361 tcagtactgg tttattgcca tagaaactgc gggcgcctcc atcataaaaa gtcagcccga
    15421 aataaccttt gcttttcgtt gagtcgagga tttttctccc gatatcgccc tgcatgacct
    15481 gataataatg atcacgatcg cggaacaaat aaggcaggct aaataattta taggaatttt
    15541 caaatgcttc catttctgcc gcattggttt ttaccagtgg aatagcgcca gaacgaatca
    15601 gctccattgt ttctcgttga gtacccaatg ttccatttgg ataaatacga atagtaatat
    15661 caccgttaga gtactctttg ttctttttcg caaagaactc catcgcttta tgaacaggat
    15721 gagacttatc ctgattatga ctcagtttta atgtcactgc ccaggactgg cttgcggata
    15781 acataagaac agcaccgata atgccagcgg atacttttat tttcatagat gttccttttc
    15841 taataaatat ggcggcaggt gccgccagtg aatgcgcatc agtagcccag tgcttttggc
    15901 aatgccatac tgatttgcgg aatataggtc accattgcca gcaccaatac catcacggca
    15961 tagaatggca gcataggttt gataatttta ttaatcggta atttagatac actacaaccg
    16021 acaaataaca cactgcctac aggcggagta caaataccga tacacatgtt cagtaccatg
    16081 acaataccaa agtgaatcgg gtccatgccc agttcggtga caacaggtaa aaatatcggc
    16141 gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc cgataatcaa caggataata
    16201 ttgataataa gcaggattat tattgggttg tcagaaatag tgttaagcaa atcgagaatc
    16261 aggaagggaa cgtcagcgtt tgacattgcc catga
//
LOCUS       DAJVYM010000088        15871 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVYM010000088 DAJVYM010000000
VERSION     DAJVYM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.089"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..1453
                     /locus_tag="OQS60_002604"
                     /pseudo
     CDS             1..1453
                     /locus_tag="OQS60_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OQS60_002605"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OQS60_002605"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS2388322.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OQS60_002606"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OQS60_002606"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS2388323.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OQS60_002607"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OQS60_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS2388324.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="OQS60_002608"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="OQS60_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS2388325.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="OQS60_002609"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="OQS60_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS2388326.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OQS60_002610"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OQS60_002610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS2388327.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OQS60_002611"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OQS60_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS2388328.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OQS60_002612"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OQS60_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS2388329.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="OQS60_002613"
     CDS             8572..9174
                     /locus_tag="OQS60_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS2388330.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OQS60_002614"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OQS60_002614"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS2388331.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="OQS60_002615"
     CDS             complement(9732..10472)
                     /locus_tag="OQS60_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2388332.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OQS60_002616"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OQS60_002616"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS2388333.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OQS60_002617"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OQS60_002617"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS2388334.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="OQS60_002618"
     CDS             13153..13719
                     /locus_tag="OQS60_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2388335.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="OQS60_002619"
     CDS             14074..14322
                     /locus_tag="OQS60_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS2388336.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15871
                     /locus_tag="OQS60_002620"
     CDS             14923..>15871
                     /locus_tag="OQS60_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2388337.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFE"
BASE COUNT         4033 a         4028 c         3638 g         4172 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc
    15721 tcttcgccgg actaccacgg cccgcagatt tacttctcca tgagtgacgg acaacagaat
    15781 acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct
    15841 gatgtatcac agatatggca tgcttttgaa c
//
LOCUS       DAJVYM010000089        15583 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVYM010000089 DAJVYM010000000
VERSION     DAJVYM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.090"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..2066
                     /locus_tag="OQS60_002621"
     CDS             <1..2066
                     /locus_tag="OQS60_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2388338.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2082..3116
                     /locus_tag="OQS60_002622"
     CDS             2082..3116
                     /locus_tag="OQS60_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCS2388339.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3198..3536)
                     /gene="rcnB"
                     /locus_tag="OQS60_002623"
     CDS             complement(3198..3536)
                     /gene="rcnB"
                     /locus_tag="OQS60_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS2388340.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3755..4579)
                     /gene="rcnA"
                     /locus_tag="OQS60_002624"
     CDS             complement(3755..4579)
                     /gene="rcnA"
                     /locus_tag="OQS60_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS2388341.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4700..4972
                     /gene="rcnR"
                     /locus_tag="OQS60_002625"
                     /pseudo
     CDS             4700..4972
                     /gene="rcnR"
                     /locus_tag="OQS60_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5064..5160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5195..5983
                     /gene="thiM"
                     /locus_tag="OQS60_002626"
     CDS             5195..5983
                     /gene="thiM"
                     /locus_tag="OQS60_002626"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS2388342.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5980..6780
                     /gene="thiD"
                     /locus_tag="OQS60_002627"
     CDS             5980..6780
                     /gene="thiD"
                     /locus_tag="OQS60_002627"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS2388343.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6845..7663
                     /locus_tag="OQS60_002628"
     CDS             6845..7663
                     /locus_tag="OQS60_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS2388344.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7715..8461
                     /locus_tag="OQS60_002629"
     CDS             7715..8461
                     /locus_tag="OQS60_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS2388345.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8435..9400)
                     /locus_tag="OQS60_002630"
     CDS             complement(8435..9400)
                     /locus_tag="OQS60_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS2388346.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9397..10401)
                     /locus_tag="OQS60_002631"
     CDS             complement(9397..10401)
                     /locus_tag="OQS60_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS2388347.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10398..11675)
                     /locus_tag="OQS60_002632"
     CDS             complement(10398..11675)
                     /locus_tag="OQS60_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2388348.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11932..12984
                     /gene="fbaB"
                     /locus_tag="OQS60_002633"
     CDS             11932..12984
                     /gene="fbaB"
                     /locus_tag="OQS60_002633"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS2388349.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13303..14157
                     /gene="gatY"
                     /locus_tag="OQS60_002634"
     CDS             13303..14157
                     /gene="gatY"
                     /locus_tag="OQS60_002634"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS2388350.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14186..15443
                     /gene="gatZ"
                     /locus_tag="OQS60_002635"
                     /pseudo
     CDS             14186..15443
                     /gene="gatZ"
                     /locus_tag="OQS60_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15453..>15583
                     /locus_tag="OQS60_002636"
     CDS             15453..>15583
                     /locus_tag="OQS60_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS2388351.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3988 g         3779 t
ORIGIN      
        1 gtggctatgt tccaccggaa aactgggagc ggggtattaa tgcgttttat acctcttatt
       61 atgtgagtca gtattacagt gactataaag cgtcgggtaa tagtaagagt acatatgtac
      121 gttttaacag cgggttaaat ttactggggt ggcaactgca ttctgatgcc agtttcagta
      181 aaacaaataa caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg
      241 cccaacttct cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt
      301 ctgttcgctt cagaggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac
      361 aaaattttac gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac
      421 agaatggttt tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt
      481 tgcagttggc cggtggtgga gcagatcttg atgtcagcgt gaaagaggcg gacggttcgg
      541 taaccaccta tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga
      601 aatatgattt tgcggcgggt cgtagccata ttgaaggggc gagcaaacat agtgattttg
      661 tccaggcggg ttatcagtat ggttttaata acttattgac gttgtatggt ggctcgatgg
      721 ttgcgaataa ttattacgcg ttcactttgg ggactggctg gaatacacgc attggtgcca
      781 tttccgtcga tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc
      841 aaagttatca aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg
      901 cggcctggcg ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca
      961 ataaagataa ttatcgccgt gatgaaaacg atgtctatga cattgccgat tattaccaga
     1021 atgattttgg tcgcaaaaat agcttttccg ccaatatgag ccagtcattg ccagaaggtt
     1081 gggggtctgt gtcattaagt acgttatggc gagattactg ggggcgtagc ggcagtagta
     1141 aggattatca gttgagttat tccaacaacc tgcgacggat aagctatacc ctcgcggcaa
     1201 gccaggctta tgacgagaat catcatgaag agaaacgttt taatattttt atatcgattc
     1261 cctttgattg gggtgatgac gtttcgacgc ctcgtcggca aatatatatg tctaactcaa
     1321 cgacgtttga tgatcagggg tttgcctcaa ataatacggg attatcagga acagtaggga
     1381 gtcgggatca gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga
     1441 cagctggagc gaatttaacc tggaacgcgc cggtggcaac agtgaatggc agttatagtc
     1501 agtcgagtac ttatcgacag gctggagcca gtgtttcagg gggcattgtc gcctggtcgg
     1561 gtggcgttaa tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa
     1621 ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg
     1681 tatacgacgg aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg
     1741 atagcgaagc agaattacgt ggcaaccgga aaattgccgc accttatcgc ggcgcggttg
     1801 tactggttaa ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg
     1861 atgggcaacc attaacgttt ggttatgaag tcaatgatat ccacggtcat aatattggcg
     1921 ttgtcggcca gggaagccag ttatttattc gcaccaatga aataccgcca tcggttaatg
     1981 tagcaattga taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg
     2041 aaagtagaaa ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc
     2101 tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat
     2161 ctgttcagca caatttggtt gttcaggggg attttgcact tactcaaaca caaatagcga
     2221 catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga
     2281 gttcgtcgga tattattgtt gggctttata acgataccat aaaattaaat ttacattttg
     2341 aatggaccaa taaaaacaac atcacgttgt caaataatca gaccagtttc accagtggtt
     2401 attcagttac ggtgacacct gcggccagta atgcaaaagt gaatgtttct gcggggggcg
     2461 gcggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg
     2521 ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg gatgcatgtg
     2581 taaatagcta cagaaatgca ttggcacaaa atgcaggtgt ctattccttt aatctgacat
     2641 tgtcatacaa cccgataacc acaacctgca aaccggacga tttattaatt actttagaca
     2701 gtattcccgt ttcacaatta ccagccacag gtaacaaagc aacaataaat agtaaacaag
     2761 gggatattat tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca tcacggaaaa
     2821 tgcaggtgta tttatcaagt tctgatttgt tagccggcag taacacgatc ctgaaaggtg
     2881 cagaaaataa tggagtcgga tttatgcttg aaagcaatgg ttctccagtc acatttttaa
     2941 atatcactga taacagcaaa ggatacacaa atttaaaaga aattgcggca aagtcaaaac
     3001 ttacagatac aacggtttca attccgataa ctgccagtta ctacgtctac gatacaaaca
     3061 aagttaaatc tggcgcactg gaggcaaccg cattaatcaa cgtgaaatac gactaataac
     3121 aagcaagacg agcaagtggc taataataaa aaataacaag gtgtctgcat ttccccctca
     3181 tgatgagggg gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt
     3241 ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg
     3301 gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa tgttgtactc atcggtgcgg
     3361 tacatctctg ggacggtatc acctggtttg aaatgcttaa agtcagcaat gaaactactt
     3421 aactcatact tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag
     3481 gcggcactgg taaccagcaa aagcacaccc agcaacatct tatttttaat ggtcataaac
     3541 tctccacttt ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg
     3601 acaagcgata ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc
     3661 ctaaaagcat ccttagcgag cagagagtca gcggaaatag ctgatttaag aatcatcctg
     3721 ggctgaagat aacatgcaag cctaaaggat tgagttatcg cattatgccc atgaagccat
     3781 gtacgcccat atacacaccg actaagccaa tcaacagact ggaaaaatag ggggcgcgtt
     3841 tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc
     3901 caacgcctac ggtgacaagc gttaacgcca ggccaatgct gaaactgacg accagtgttg
     3961 cgcccagtgt cagggctttc aactgaatgc atatcaacag cacggtaatt gctgccgggc
     4021 aggggataag gccaccggtt aagccaaata gcaaaatttg ccagttggtg acctctctac
     4081 catcaaagcg tcgtttaatg tcattggcat gggctcgtgc atgggcatcc tgatactcgc
     4141 catgttcgtg atggtgatga tgtccatggt cgtggtgatc ttcgtgatcg tgatgatgat
     4201 gctcataatc atactcgtgc atattctcca gccagttgcg ttcgccgcgc caggtacgcc
     4261 agaacatcca gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt
     4321 ctgctgattg agcagtaaag cgcttgctga tcaccatccc gccaaaggca attaaccaga
     4381 ccactgcggt atgcgaaata gttgctgcca gtccgagcat caccgcttgt ttaatggtgc
     4441 ctttgatggc gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat
     4501 gaagcgcacc aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa
     4561 gagttgtaaa ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact
     4621 ccccccagta cctgatacta ccccccagta gattaatagt gctatgatat tttcatattc
     4681 ttgttaacca ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg
     4741 tgccagtaag attcagggct aggtcgtggc gctcaagaaa atgctcgacg agccgcatga
     4801 atgcgctgca gttttacaac agattactgc tatccgtggc gcggtaaacg gtctgatgcg
     4861 ggaagtgatt aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg
     4921 tgaagaagat ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa
     4981 cgcgattgta aaactgccgt ttttcctcgt ttacaacgcg tgcgctggac attaccatcc
     5041 tcctctgcga tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga
     5101 gaaatacccg tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg
     5161 tcattgcttc ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgggttcagc
     5221 gcaatctgcg cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac
     5281 caatgatgtg gtgcaaacct ttaccgctaa taccttgctg gcgctcggtg catcgccagc
     5341 gatggttatc gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat
     5401 taacgttggc acactgacgc agccacgcgc tcaggcgatg cgtgctgccg ttgagcaagc
     5461 aaaaagctct caaacaccct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg
     5521 ccgccatttt tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc
     5581 ggaaatcatg gcattagctg gcattgctaa tggtggacgg ggagtggata ccactgacgc
     5641 cgcagctaac gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt
     5701 ggtcactggc gaggtggatt atgttaccga tggccatcgc gccgttggca ttcacggtgg
     5761 cgatccgtta atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc
     5821 ctgctgtgcg ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa
     5881 acaagccgga gagcgcgcag tcgccagaag cgaggggcta ggcagttttg ttccacattt
     5941 ccttgatgcg ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg
     6001 acgattgccg gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc
     6061 tcggcacttg gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt
     6121 ggcgtacagt cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg
     6181 ttcagcgatg tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt
     6241 gaagcggtgg cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt
     6301 atgctggcaa aaagcggcga cccactgctt tcaccttcgg cagttgccac gctacgcagt
     6361 cgattattgc cacaggtttc attaataacg ccaaacttgc ccgaagcagc cgccttgctc
     6421 gacgcgccac acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg
     6481 atgggctgtg gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac
     6541 tggctgttta cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac
     6601 acccacggta ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca
     6661 aactgggctg acaccgtaca ggaggcaaaa agctggcttt catcggcgtt agcccaggcc
     6721 gacacgctgg aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga
     6781 gcaccgtact atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa
     6841 accaatgcag ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg
     6901 gctaatttcc atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc
     6961 agttcagatt acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat
     7021 tcgtattcct gccagttaca ccattcacgg aatagatgtt tcgcgctggc aggagcggat
     7081 cgactggcag cgtgtggcaa aaatgcgcga taacggtatt cgcttacaat ttgcctttat
     7141 taaggcgacg gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaactaag
     7201 ccgcgaaaat ggcttgctgc gcggggcata tcattatttc tccccgtcgg tagcagcacc
     7261 ggttcaggtg cgattatttc tgcaaacggt agatttctca caaggcgatt tccctgcggt
     7321 actggacgta gaagagcgag gaaaattatc ggcaaaagaa ttacgcaaga gggtaagtca
     7381 gtggctaaaa atggtcgaaa aaagtacggg gaaaaagccg attatttact caggagccgt
     7441 tttttatcac acgaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta
     7501 ctatcaacgt cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg
     7561 tggacaggta gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggtgga
     7621 ggagttgcag ggattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat
     7681 actcccacca gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat
     7741 tgcccaactg aacgaacgta ttttagcggc ggataacacg ccgctttata ttaagtttgc
     7801 cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg
     7861 tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca
     7921 ggcgctggaa gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa
     7981 catcttcgaa tactcgttga aagaagcgcg aggtttttct cagcaggtgg tgttgcgcgg
     8041 gaaaaagccc gatacgctat gggttaacaa gcgtgtcgtg aaatgtcccg aagaagtcgc
     8101 gcagcagctg gcggtcgaag caggaagtga tgtctttttg cttaagcgta ttcgctatgt
     8161 cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt
     8221 tgatgccatc gggatttcac tttatgacta tttccgcagc cagcacattt acccacaacg
     8281 tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt
     8341 agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc
     8401 tattgagtac agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta
     8461 gttcctcgcg cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg
     8521 cattgcccag taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc
     8581 cacctgcatg actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa
     8641 tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca
     8701 acggcgcggc aaatttctcc tgccattgct cgccaagtgt tgttatctct gcggataaag
     8761 caaaacgttc ggcggcaatc tcagcctctt gacgattgag cgacactaaa ggtcgacagg
     8821 ccatgatccg cgccagtaat gcatccggga tatcgccaat acgtgggcca aaatcgataa
     8881 acggcgtcac gtcttgcagc tcttccagcc attccactaa caattcgccg cagggcgagg
     8941 ccagttgata accggaaaaa tagagcaggc tgccaggcgc aacggttaat cgcgccagcc
     9001 actggcgatt ccactgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg
     9061 gctcaaccag cgccagacac cagccgttat caccttcggc gttatcgatc aggctgatta
     9121 agccctcttt tgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca
     9181 aggcattacc cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc
     9241 cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat
     9301 aagcgtcggc gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg
     9361 ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg
     9421 atatttcgcc agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagttc
     9481 cgcctttaac gcaggatcga tagcgttaac gccatgcaac gcgccgcaaa ttgccgtcgc
     9541 catagcaccg atggtgtctg tgtcgccgcc aaggttagcg cacaggacgg cgcagcgatt
     9601 cgggtcggtt tgtgctagtt caaccagcgc aatggcgcac ggaacggact caatagtgct
     9661 ggtacctgcg ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt
     9721 gcgcacaatt ttcagcgcaa tctccagccg cgccgccagt gaggcgctga acgtagtgat
     9781 gcgtttttgc tgtgcatgtc gcgcaattga aggcagggaa tcaacaatcg ctgaccagct
     9841 ttctccgtca atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag
     9901 atcggattta tgggtcgggc tggacgccag cgctacatca tcaataaagg aatcaacatc
     9961 acgcgccgga agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggtcacgcc
    10021 gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga
    10081 ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc agcgcccagt cgagaatatt
    10141 acgcccaatc agatccggat cgatcttgcc ttcacgttcc agtaacgcat ccgccagaca
    10201 cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt
    10261 ctcctttggt ccaggaagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg
    10321 tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc
    10381 gagaatacgt tctgttttca tttaacttcc ccttgtgtca acgcaatatc gcgatcatcg
    10441 accttgatag ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg
    10501 ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg
    10561 tttaccggtt cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg
    10621 agcaaactgc cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata
    10681 tgcacggggg cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt
    10741 acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgtacta
    10801 ccgtaaataa agaagccata gcggatcgca gcggtgacca gaccaagcaa taataccttt
    10861 ttgataccaa agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac
    10921 tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg
    10981 gcaaagatgt aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg
    11041 aggaagtttt tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatatcc
    11101 attttgccgg tgctttttgg tggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga
    11161 gcagaacttc cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc
    11221 agtatttgcg gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc
    11281 acacgaatgc gcgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta
    11341 gtcagcgcaa tggtcggcat ataggttaga gagtaagcca gcagtaacgg aaagaacccg
    11401 gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg cgcctgcgaa catcaatacc
    11461 gccagcactt tttgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac
    11521 aggatcgccg caatggcggt acaggcatac gaccagccaa tttctccggc actaaaaccg
    11581 cttttactta accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca
    11641 aacatcatga acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa
    11701 tgtaaggtac gcgatgacaa taccacttaa caataccttt taaatacctt tgcggtgaaa
    11761 acttgaccgc gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca
    11821 tccaaaagat ggaaaaactc gcccgtaacg accaggatta tgattgcccg caaaaggcgg
    11881 gccaggacaa tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat
    11941 attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgacaatt
    12001 ccttctgacc agctttatct ccccggacat gactacgtag accgcgtaat gattgacaat
    12061 aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct
    12121 ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct
    12181 tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa
    12241 gcgggctgta actgtgtggc gtcaacttac ggcgtgttgg cgtcggtatc gcggcgctat
    12301 gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat
    12361 acctacgatc aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg
    12421 gttggtacga ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct
    12481 gcggcctttg aacgcgcgca cgagctgggc atggtgaccg tgctgtgggc ctatttgcga
    12541 aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct gaccggtcag
    12601 gcaaaccatc tggcggcaac catcggtgca gatatcgtca aacaaaaaat ggcggaaaat
    12661 aacggcggct ataaagcaat taattacggt tacaccgacg atcgtgttta tagcaaattg
    12721 accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg
    12781 gctgggttga taaactccgg cggtgctgcg ggcggtgaaa ctgacctcag tgatgcagtg
    12841 cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc tgattcttgg acgtaaagcg
    12901 ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg ccgtgcagga cgtttatctc
    12961 gatagcaaaa ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg
    13021 ataaggcgtt tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc
    13081 atgcctacat agcactgcca cgtatgtcgg aaaaggcgtt tacaccgcat ccggcataaa
    13141 aacacgcgca ctttgctacg gcttccctat cgggaggccg tttttttgcc tttcactcct
    13201 cgaacagttt tcatattgtc gttttgtgat cgttatctcg acatttaaaa acaaatcatt
    13261 tcattatatt ttgaaatcga aaacaagcaa caggatatga aaatgtacgt ggtatcgaca
    13321 aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac
    13381 aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc
    13441 atcgccggaa cgcctggcac attcacttat gctggtacag aaaatctgtt ggcgctggtc
    13501 agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa
    13561 tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg
    13621 catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc
    13681 tttgatgtca gcgtcgaagc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa
    13741 gtcaatgaag ccgatgtttt ttataccaac cccgctcagg cgcgtgaatt tgccgaggca
    13801 accggaattg attccctggc ggtcgccatc ggcacggctc atgggatgta tgccagcgca
    13861 ccggcgcttg atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg
    13921 ctgcatggcg cgtcagggtt atcgactaag gatattcagc aaaccatcaa actggggata
    13981 tgcaaaatca acgttgcaac ggagctgaaa aatgccttct cgcaggcgtt aaaaaattac
    14041 ctgaccgagc accctgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca
    14101 atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc
    14161 acctgccatt taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga
    14221 acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc
    14281 atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca
    14341 atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc
    14401 cgataaagtc ggatttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa
    14461 ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc
    14521 atatgttcgt gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga
    14581 tcccataccg ttagcaccag aaacggttgc ggaacgagct gctttgcttt gctgcggaaa
    14641 gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg
    14701 ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg
    14761 ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa
    14821 cacgcgtgat tgccatcgtg gtgcagccgg gcgtggaatt tgatcacagc aatattatcc
    14881 attatcagcc gcaggaagcg cagccgctgg cgcaatggat agaaaacacc cgaatggttt
    14941 atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc
    15001 actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg
    15061 cactggcaca aattgagcag gaacttatcg cccctgaaaa tcgcagcggt tgcctggcgg
    15121 taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat cgtacgggtt
    15181 ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc
    15241 cgcatagtcg gattaaaaat agcgtcgaaa cgatgatggt gaatcttgaa ggcgtggaca
    15301 tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg
    15361 aattatcagc aataccgcat cagctgatta tggataaaat ttatgatgtt ttgcgcgcct
    15421 atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa
    15481 tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg ctcgccaaag
    15541 gtgtggttca tgatacctgg ccacaggcat taattgccag aga
//
LOCUS       DAJVYM010000090        15462 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVYM010000090 DAJVYM010000000
VERSION     DAJVYM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.091"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OQS60_002637"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OQS60_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS2388352.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OQS60_002638"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OQS60_002638"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS2388353.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OQS60_002639"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OQS60_002639"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS2388354.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OQS60_002640"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OQS60_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OQS60_002641"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OQS60_002641"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS2388355.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OQS60_002642"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OQS60_002642"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCS2388356.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OQS60_002643"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OQS60_002643"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS2388357.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OQS60_002644"
     CDS             8229..8939
                     /locus_tag="OQS60_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS2388358.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OQS60_002645"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OQS60_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS2388359.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OQS60_002646"
     CDS             9636..10343
                     /locus_tag="OQS60_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS2388360.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OQS60_002647"
     CDS             10343..10891
                     /locus_tag="OQS60_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS2388361.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OQS60_002648"
     CDS             10901..12067
                     /locus_tag="OQS60_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2388362.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OQS60_002649"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OQS60_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OQS60_002650"
     CDS             13576..14229
                     /locus_tag="OQS60_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS2388363.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..>15462
                     /gene="ynjE"
                     /locus_tag="OQS60_002651"
     CDS             14296..>15462
                     /gene="ynjE"
                     /locus_tag="OQS60_002651"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS2388364.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGW"
BASE COUNT         3551 a         4203 c         4042 g         3666 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct gg
//
LOCUS       DAJVYM010000091        15461 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVYM010000091 DAJVYM010000000
VERSION     DAJVYM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.092"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            270..1388
                     /locus_tag="OQS60_002652"
     CDS             270..1388
                     /locus_tag="OQS60_002652"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS2388365.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="OQS60_002653"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="OQS60_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="OQS60_002654"
     CDS             1767..2135
                     /locus_tag="OQS60_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS2388366.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="OQS60_002655"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="OQS60_002655"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS2388367.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="OQS60_002656"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="OQS60_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS2388368.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OQS60_002657"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OQS60_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS2388369.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OQS60_002658"
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OQS60_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS2388370.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OQS60_002659"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OQS60_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS2388371.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OQS60_002660"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OQS60_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS2388372.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OQS60_002661"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OQS60_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS2388373.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="OQS60_002662"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="OQS60_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS2388374.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..14193
                     /gene="cusS"
                     /locus_tag="OQS60_002663"
     CDS             12745..14193
                     /gene="cusS"
                     /locus_tag="OQS60_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS2388375.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            14930..>15461
                     /locus_tag="OQS60_002664"
     CDS             14930..>15461
                     /locus_tag="OQS60_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2388376.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKST"
BASE COUNT         3738 a         4102 c         3793 g         3828 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gagccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtaacccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcaa tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttctctggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg
    13141 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
    13201 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg
    13261 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct
    13321 gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg
    13381 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
    13441 gacccgcaga ccgagcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    13501 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa
    13561 attcgcacgc cgattaccaa tctcattacg caaacggaaa tcgccctcag ccagtctcgc
    13621 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    13681 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
    13741 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta
    13801 gcggaagatc gcggcgtgga gttgcgattt gttggcgaca aatgtcaggt tgcgggcgat
    13861 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca
    13921 accagagaga caattgtagt gcgctgtcag acggtcgatc accaggtgca agttaccgtc
    13981 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctac
    14041 cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg
    14101 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca
    14161 aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt
    14221 gcatcacctc ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa
    14281 tatcgaaggc ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa
    14341 tctgtgtttc ttccaattgt agatcacaat gccctttcta aattttcatc gttataaaca
    14401 agccatctat catataaagc aatgaataat ttaatcgcga ttaatcatca ttaattgtta
    14461 tattattcac tcaatctatt tgtcaagtga atttgataaa ttcctaattt aaaatatcca
    14521 ctattgagcc agtatttaac aacgttaaga tgtgcaatgt gcagatcatg ttatcagggt
    14581 ggagcggtag catatatttc ttatgcaatc attcatttta ataatgttaa ttggattatt
    14641 tattgctgtg aataaaaata tattatgaat attggtttaa ctttgcgttt ttgttactcg
    14701 ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca agaggttgag cttgtgatta
    14761 tttttttgac tgaacagaca cccgtttctg taaagtaata tttaaacaaa attctcacat
    14821 aaccatagca ttttctgttc cttccctaat aacgtctgaa gtcgtcccgc ttatcacatt
    14881 atccgcagtt gtacggtacg gatcattatt cattttactg gaggttgcta tgtcaaccgg
    14941 attacgtttc acactggaag tggacggcct gccgccggac gcttttgcgg tggtttcctt
    15001 tcatctgaac cagtcactct cttcgctctt ttccctcgat ctctccctgg tcagccagca
    15061 gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga cgatatggca
    15121 gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc tggtttgaac tcggggagaa
    15181 cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg ccgctgtggc gtgccggtct
    15241 gcgccagaac ttccgtatct tccagaacga ggacatcaaa agcatcctcg gcacgatgtt
    15301 gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc gagccgcatc cttcccgtga
    15361 gttttgtgtc cagtacggtg agactgatta cgatttcctg tgccggatgg cggcggagga
    15421 aggcatcttc ttttatgagg agcatgctta caaaagtacc g
//
LOCUS       DAJVYM010000092        15235 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVYM010000092 DAJVYM010000000
VERSION     DAJVYM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.093"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..749
                     /locus_tag="OQS60_002665"
     CDS             <1..749
                     /locus_tag="OQS60_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS2388377.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="OQS60_002666"
     CDS             complement(790..1722)
                     /locus_tag="OQS60_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS2388378.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OQS60_002667"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OQS60_002667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS2388379.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OQS60_002668"
     CDS             complement(5254..5886)
                     /locus_tag="OQS60_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS2388380.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OQS60_002669"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OQS60_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS2388381.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OQS60_002670"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OQS60_002670"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS2388382.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OQS60_002671"
     CDS             complement(8729..8839)
                     /locus_tag="OQS60_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS2388383.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQS60_002672"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQS60_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS2388384.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQS60_002673"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQS60_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS2388385.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQS60_002674"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQS60_002674"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS2388386.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OQS60_002675"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OQS60_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS2388387.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3914 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgct
//
LOCUS       DAJVYM010000093        15231 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVYM010000093 DAJVYM010000000
VERSION     DAJVYM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.094"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..563)
                     /locus_tag="OQS60_002676"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="OQS60_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(797..2217)
                     /gene="espX1"
                     /locus_tag="OQS60_002677"
                     /pseudo
     CDS             complement(797..2217)
                     /gene="espX1"
                     /locus_tag="OQS60_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="OQS60_002678"
     CDS             complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="OQS60_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS2388388.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3068..3286
                     /locus_tag="OQS60_002679"
     CDS             3068..3286
                     /locus_tag="OQS60_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS2388389.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3294..4235
                     /gene="ribF"
                     /locus_tag="OQS60_002680"
     CDS             3294..4235
                     /gene="ribF"
                     /locus_tag="OQS60_002680"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS2388390.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4278..7094
                     /gene="ileS"
                     /locus_tag="OQS60_002681"
     CDS             4278..7094
                     /gene="ileS"
                     /locus_tag="OQS60_002681"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS2388391.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7094..7588
                     /gene="lspA"
                     /locus_tag="OQS60_002682"
     CDS             7094..7588
                     /gene="lspA"
                     /locus_tag="OQS60_002682"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS2388392.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7713..8162
                     /gene="fkpB"
                     /locus_tag="OQS60_002683"
     CDS             7713..8162
                     /gene="fkpB"
                     /locus_tag="OQS60_002683"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS2388393.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8164..9114
                     /gene="ispH"
                     /locus_tag="OQS60_002684"
                     /gene_synonym="lytB"
     CDS             8164..9114
                     /gene="ispH"
                     /locus_tag="OQS60_002684"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS2388394.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9180..10094
                     /gene="rihC"
                     /locus_tag="OQS60_002685"
     CDS             9180..10094
                     /gene="rihC"
                     /locus_tag="OQS60_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS2388395.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10261..11082
                     /gene="dapB"
                     /locus_tag="OQS60_002686"
     CDS             10261..11082
                     /gene="dapB"
                     /locus_tag="OQS60_002686"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS2388396.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11538..12686
                     /gene="carA"
                     /locus_tag="OQS60_002687"
     CDS             11538..12686
                     /gene="carA"
                     /locus_tag="OQS60_002687"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS2388397.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12704..>15231
                     /gene="carB"
                     /locus_tag="OQS60_002688"
     CDS             12704..>15231
                     /gene="carB"
                     /locus_tag="OQS60_002688"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS2388398.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEV"
BASE COUNT         3719 a         3567 c         4013 g         3932 t
ORIGIN      
        1 tccccccagt agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt
       61 tcatcataag attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa
      121 ctgaattggt aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt
      181 aatgatacat ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt
      241 tttctgccaa tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg
      301 taaagatggt cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta
      361 aatcgagtgg ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg
      421 tgtctggtat cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc
      481 agcggtgtat tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta
      541 cccgattatg tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg
      601 atgtgaagtt gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga
      661 agagcaaggt gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg
      721 ttttttattt ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc
      781 tctttctttg agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt
      841 tgatgctctg aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact
      901 tattaagttc tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta
      961 ccaatggtat caaaatttcg aaacgggacg gtttgtcatc aatacttgcc agggttttga
     1021 atactcctgt gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag
     1081 gcataatatc ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata
     1141 tgttgctttt tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata
     1201 agtcgctttc aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa
     1261 ggttgctctt ttctattgtt atatttgata gcaacatttt atgtatatta atattaagag
     1321 cagtcaaata ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg
     1381 gtaataaaag tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt
     1441 tattagtggc catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca
     1501 cgcttttaaa taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa
     1561 agttctgcca cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt
     1621 gctcattgta tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa
     1681 gatgactttt tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat
     1741 cataaaatga ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg
     1801 ggtatatttc ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt
     1861 ggtatatctt aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc
     1921 taagtaatgt gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt
     1981 acatctataa aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg
     2041 agatggttta ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt
     2101 tttatactcg caaaatactt ttactaactc attttgtctt gttttattta ttattacatc
     2161 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat
     2221 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
     2281 aaaaatagct cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt
     2341 gttgttaatt atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg
     2401 aatttaattc aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc
     2461 acattctttg taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc
     2521 gatggttcta cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa
     2581 agccagcatc ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc
     2641 agcaggcata aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg
     2701 attaagccag tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt
     2761 tgtggatcag acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt
     2821 tctgtgcagc agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac
     2881 gcatcataga gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct
     2941 tcttagctga tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt
     3001 caaaggccga ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc
     3061 gccgttaatg tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg
     3121 gtgtgaatac agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat
     3181 ttgcgccatg gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac
     3241 cttgaccgct gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc
     3301 tgatacgcgg catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg
     3361 gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg caggaagaag
     3421 ggcgcaagcg caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt
     3481 ttgctaccga taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg
     3541 cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa
     3601 ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg
     3661 taggggatga tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag
     3721 ctagcatgga atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc
     3781 gtattagcag caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa
     3841 gtttactggg gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc
     3901 gcactatagg tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag
     3961 gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca
     4021 tcggaacacg cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg
     4081 ttgcaatgga cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg
     4141 agcagcgatt tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg
     4201 cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac
     4261 acggaaccga gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt
     4321 tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg
     4381 atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg
     4441 atggccctcc ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga
     4501 aagacattat cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct
     4561 gggactgcca cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg
     4621 agaaattcac cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg
     4681 acggtcaacg caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc
     4741 tgaccatgga cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca
     4801 acggtcacct gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc
     4861 tggcggaagc ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc
     4921 aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa
     4981 tctcgctggt gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta
     5041 ttgcacctga tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga
     5101 aagatctggt tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca
     5161 cggtaaaagg tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg
     5221 ttccggcaat cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg
     5281 cgcctggcca cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta
     5341 acccggttgg cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga
     5401 acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc
     5461 acgttgagaa aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct
     5521 tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac
     5581 tgaaagagat caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga
     5641 tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt
     5701 cactgttcgt gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag
     5761 aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag
     5821 agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt
     5881 ttgactccgg atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg
     5941 cagcggacat gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa
     6001 tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta
     6061 ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg
     6121 atgtgatgaa caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca
     6181 ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta
     6241 tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata
     6301 tggtgaaacc ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg
     6361 cacaggaaga catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc
     6421 tgatgcgctt ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc
     6481 agtacaccgc caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca
     6541 tcgcagaagc gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt
     6601 ggggctacct gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc
     6661 tgtttggcct ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag
     6721 tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct
     6781 cgctggaagc ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc
     6841 tgggcgatga attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg
     6901 acgcacctgc tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta
     6961 aagccgaagg tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg
     7021 cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac
     7081 gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt
     7141 ggtagtcgtg ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct
     7201 gggggatacg gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc
     7261 ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc
     7321 gattggtatt agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct
     7381 aaacaatatc gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct
     7441 gtggcacggc ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac
     7501 cttcaacctt gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt
     7561 tttgccttct agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct
     7621 tatccggcct acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg
     7681 gcaaaaatcc tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     7741 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     7801 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     7861 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     7921 cgtttggcgt gccgtcaccg gacctgattc agtacttttc ccgccgtgaa tttatggatg
     7981 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     8041 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     8101 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt
     8161 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     8221 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     8281 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     8341 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     8401 gtacgtaacg aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     8461 accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc
     8521 ggccacgccg ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg
     8581 ggaatgtatc tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag
     8641 aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     8701 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     8761 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     8821 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     8881 aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     8941 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg
     9001 gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac
     9061 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     9121 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     9181 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     9241 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     9301 cggttgagaa aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc
     9361 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     9421 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga
     9481 taccagcgtt tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg
     9541 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac
     9601 cgtatattcg tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa
     9661 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     9721 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     9781 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     9841 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     9901 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     9961 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
    10021 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
    10081 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
    10141 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
    10201 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
    10261 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
    10321 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
    10381 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
    10441 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
    10501 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
    10561 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
    10621 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
    10681 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
    10741 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
    10801 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
    10861 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat
    10921 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt
    10981 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt
    11041 ctttttgata tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat
    11101 ggtgcaaaaa taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt
    11161 atttttagtg ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc
    11221 tatttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
    11281 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg
    11341 tcgcttaatg cctgtaaaat atgcatgagc cacaaaataa tataaaaaat accgccatta
    11401 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
    11461 gcagatttgc attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat
    11521 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
    11581 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
    11641 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
    11701 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
    11761 tacatgcaca aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata
    11821 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
    11881 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
    11941 gcgataaccc ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg
    12001 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
    12061 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
    12121 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
    12181 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
    12241 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
    12301 tccagaaatt cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc
    12361 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
    12421 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
    12481 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    12541 gtacgttaca gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg
    12601 aagccagccc tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg
    12661 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    12721 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    12781 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    12841 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    12901 cgagccgatt cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt
    12961 gctgccaacg atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg
    13021 cgtgttggaa gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc
    13081 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    13141 cggtatcgca cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg
    13201 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga
    13261 agagtttgaa gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat
    13321 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    13381 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga
    13441 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    13501 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    13561 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    13621 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    13681 ggcggtgggt tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc
    13741 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    13801 attcgctggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    13861 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc
    13921 gactggattc gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg
    13981 cgaactgaaa gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg
    14041 cctgtctgtg gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat
    14101 tgaagaactg gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc
    14161 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc
    14221 gggcgtgcgc gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta
    14281 taagcgcgtg gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac
    14341 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg
    14401 cggcggcccg aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc
    14461 gctggcgctg cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    14521 ctccaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    14581 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    14641 cccgctgaaa ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc
    14701 ggatgctatc gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa
    14761 actgaaacaa ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa
    14821 agagattggc tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga
    14881 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa
    14941 cgatgcgcca gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc
    15001 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    15061 cgtgcactcc ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca
    15121 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    15181 gaacgtgcag tttgcggtga aaaacaacga agtctacctg attgaagtta a
//
LOCUS       DAJVYM010000094        15110 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVYM010000094 DAJVYM010000000
VERSION     DAJVYM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.095"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            373..462
                     /locus_tag="OQS60_002689"
                     /pseudo
     CDS             373..462
                     /locus_tag="OQS60_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(463..1521)
                     /gene="modC"
                     /locus_tag="OQS60_002690"
     CDS             complement(463..1521)
                     /gene="modC"
                     /locus_tag="OQS60_002690"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS2388399.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1524..2213)
                     /gene="modB"
                     /locus_tag="OQS60_002691"
     CDS             complement(1524..2213)
                     /gene="modB"
                     /locus_tag="OQS60_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS2388400.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2213..2986)
                     /gene="modA"
                     /locus_tag="OQS60_002692"
     CDS             complement(2213..2986)
                     /gene="modA"
                     /locus_tag="OQS60_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2388401.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3152..3301)
                     /gene="acrZ"
                     /locus_tag="OQS60_002693"
     CDS             complement(3152..3301)
                     /gene="acrZ"
                     /locus_tag="OQS60_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS2388402.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3430..4218
                     /gene="modE"
                     /locus_tag="OQS60_002694"
     CDS             3430..4218
                     /gene="modE"
                     /locus_tag="OQS60_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS2388403.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4286..5758
                     /gene="modF"
                     /locus_tag="OQS60_002695"
     CDS             4286..5758
                     /gene="modF"
                     /locus_tag="OQS60_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS2388404.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6019..7035
                     /gene="galE"
                     /locus_tag="OQS60_002696"
     CDS             6019..7035
                     /gene="galE"
                     /locus_tag="OQS60_002696"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS2388405.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7045..8091
                     /gene="galT"
                     /locus_tag="OQS60_002697"
     CDS             7045..8091
                     /gene="galT"
                     /locus_tag="OQS60_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS2388406.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8095..9243
                     /gene="galK"
                     /locus_tag="OQS60_002698"
     CDS             8095..9243
                     /gene="galK"
                     /locus_tag="OQS60_002698"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS2388407.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9237..10277
                     /gene="galM"
                     /locus_tag="OQS60_002699"
     CDS             9237..10277
                     /gene="galM"
                     /locus_tag="OQS60_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS2388408.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10480..11232
                     /gene="gpmA"
                     /locus_tag="OQS60_002700"
     CDS             10480..11232
                     /gene="gpmA"
                     /locus_tag="OQS60_002700"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS2388409.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11391..12443)
                     /gene="aroG"
                     /locus_tag="OQS60_002701"
     CDS             complement(11391..12443)
                     /gene="aroG"
                     /locus_tag="OQS60_002701"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS2388410.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            12759..13139
                     /locus_tag="OQS60_002702"
     CDS             12759..13139
                     /locus_tag="OQS60_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS2388411.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13253..14194
                     /gene="zitB"
                     /locus_tag="OQS60_002703"
     CDS             13253..14194
                     /gene="zitB"
                     /locus_tag="OQS60_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS2388412.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14191..14910)
                     /gene="pnuC"
                     /locus_tag="OQS60_002704"
     CDS             complement(14191..14910)
                     /gene="pnuC"
                     /locus_tag="OQS60_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS2388413.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(14948..>15110)
                     /gene="nadA"
                     /locus_tag="OQS60_002705"
     CDS             complement(14948..>15110)
                     /gene="nadA"
                     /locus_tag="OQS60_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS2388414.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3816 a         3984 c         3771 g         3539 t
ORIGIN      
        1 tgacccaccg ccatttcatg aaatccgaag tttatccggt cgcctgcact cagcagaaaa
       61 aggcggtgat ttcactcagg cgctccttgg acatcgttca tccagcatga cggataaata
      121 ccgtgacgga cgagggaggg aatggaaaga catttaaaat taatttcgaa tgatttcgaa
      181 acaccacttg taattaattg attataaatg attaattcct aatgggcatt tgcttcatga
      241 atgctccttt gcatttagca actgactgta acagctaaaa ttagtcgctt ttggcggtga
      301 atgctgaatt aaaacgcaga attttgtccg atagtggtat cggtgtacca ttcgcgcaga
      361 cgaaaattct taaatgacgc ggtaaaagcg cgcgccaaca ttgtgattgg tgataacacc
      421 accgacagca ttgcccagtt catttatagc cacctgattt aatcaggcgg ttatcgacac
      481 acttttaatt tgcgcgtaca gccacaggcc aggtttgatc gccagttcat ccctggccca
      541 cgggctgata cgcgcccaca gcgttttacc gccgacttcc agctccactt ccacctggcc
      601 gttgtcgtca taactattaa caacttttgc ccgcaatacg ttacgaatgc tggtttgctg
      661 cggcggttgt aaaaccagag aaacatcgga agcctgaatg cggatacgta gcgcagcttg
      721 cagcggttcg tccagcttat tgacccacaa atgctgatcg cccagcgcca gcgcggtcat
      781 cgcgtaatgc ggatgatgct caagtaccgt cactttcaga atgctacttt gttgctcttt
      841 cggcagccac ggattcatca cgctactgcc ccacacttcc tccagcgcgc caaaggcttt
      901 cacctgaccg ttttccagta ccatcactct gtctgccaga tggaggatct catccagcga
      961 atggctgaca tacaacatcg gaatgttgat ttcccgtgtc agccgttgca gataaggcaa
     1021 cagttcgcgt ttacgcggaa tatccagtga cgccagcggt tcatccagca acagcaattc
     1081 cggtgctgtc agcaaagccc gaccaatcgc cacgcgctgt ttttcgcctc cggacaggct
     1141 gcctggtaaa cggtcaagca acggttcaat gcctaaaagc gccaccagct tatcgaactg
     1201 atcgaccata cttttcgaca tgccgtagcg cagattgcca cgcactttgt aatgcgggaa
     1261 cagccgcgca tcctgaaaaa catagccaac gcgacgcttt tccggcgtca ggcagatacc
     1321 tttttcggca tcatttagta cccgcccatt gaggacaatc cgcccttttt gcgggcgcgt
     1381 cagtccactg atggcgttaa tcagcgaagt ttttccggca ccggagacgc caaagatagc
     1441 agtgatgcca ttggcgggca gtgtttcatt aatggtcagg caatggttgc ccaacgtctg
     1501 ggaaaaattc agctccagca tgattagcgc cccgcccgtt cacggctgat tctggccagc
     1561 cattctgaaa tcaacaggga gatcatcgcc agcgcaatag aaataatgca cagtctcgcc
     1621 gcgccacttt ccccgccggg ggtctggatc agggtataca tggcagaggg aatggttcgc
     1681 gtttcgccag gaatgttcga cacaaaggtg atggttgcac caaactcacc gagagaacga
     1741 gcaaaagcca gtaccgtacc aacaataatt cccggtaagg tcagcggtaa cgtgatagta
     1801 aagaaaacgc gccagcgccc ggcacctagt gttcttgcgg cctgttcgag tttgacatca
     1861 accccttcca gcgccagacg aattgcccgc accatcagcg gaaacgacat gacggcggca
     1921 gcgagaaccg cgccgcgcca gctaaaggcg aaggtaatac caaaccagtc atacagacgt
     1981 tcaccgataa atccgcgccg tcccatcgaa actaataata agtaaccgac gaccacgggc
     2041 ggtaacacca gcggtagatg cagtacgctg tcgagcagag ctttgcccgg aaacgtgcaa
     2101 cgcaccagta accaggcaaa aaagatccca aacggcaggc taaacagcac agccagggaa
     2161 gaaactttca ggcttaataa aactgcctgc cattctggat cggtcagtat cattacttgg
     2221 ttgtaaatcc gtaacgttta aagatttccg ctgcctgcgg tcccttcaga taatcataaa
     2281 atgctttcac tgtcgcattg ttatgccctt ccacaacagc aaccggatat tccacttttt
     2341 tatgtgaatc ttccgggaag gtggcaacca cttttacccc tttgctgaca actgcgtcag
     2401 aaccgtaaac aatgcccaac ggcgcttcat tgcgttcgac caacgccagc gcgccacgaa
     2461 catcttccgc cggagccagt ttcggggaga gcgtatccca tgcgcccagt ttttgcagtg
     2521 cttcttttgc ataaatgcca gcagggacat gttccggatc gccaaccgcg aggcgaccgc
     2581 cattcagcag cgaagtccag ttagttgtgc tgtcgatgat gaaatctttc tgctcgctgg
     2641 ctttcggtgc tacaacgacc aggctattgc cgagcagtgt ctggcgcgaa gccgtatcga
     2701 tcgctttttt atcaaccgca taatccatcc atttctgatc ggcagagata aacagatccg
     2761 caggcgctcc cgcttcaatc tgacgggcca gagtagatga cgaagcgaaa gaagaaacca
     2821 catccacgcc tttttctttt ttatactgcg tagcaatgtc ctgcattgcg ttagtcagtg
     2881 atgcggcggc gaacaccgtg attttccctt catctgccag tgtattgcca gcaacagcga
     2941 aagagagtgc cgccccggca aacaagttca accatttacg agccattggt aaccccttaa
     3001 tgtaacgtta tataggtgac aatgtaacgg ctagctcaga attttcccag cagttattgc
     3061 taacctttgt gaggtagata agaaaaaata tcggctggaa aagcaggaag ttgagaaaaa
     3121 gaaatgcccg actaagcggg cattcaggga atcaatgatt ttgtccgggc tggtcttttt
     3181 taccaacacc agaaaagatg ttgaatactt caccaagacc gtaaatcaga cccaggatga
     3241 tggccatcac gacaggtacc atgattacgg cgaataccag actttttaat aactctaaca
     3301 tggtcaactc cagatatagt catgagacta ttctaaccgc taagcacaga aaagcactcc
     3361 ccttttgtgc ggtcagcttt gcgtggcgtt cgttttccgt cacaataaga cttttgccag
     3421 gacattgtta tgcaggccga aatccttctc acccttaagc tccaacaaaa attattcgcc
     3481 gacccgcgcc ggatttcgct actaaaacac attgcgcttt ccggttccat tagccaggga
     3541 gcgaaagatg ccggtatcag ctataaaagc gcctgggatg ccattaacga gatgaatcag
     3601 ttaagcgagc atattctggt tgagcgcgca acaggcggta aaggtggcgg cggcgcagta
     3661 ctgacccgct atggtcagcg actgattcag ctctatgact tactggcgca aatccagcaa
     3721 aaagcctttg atgtgttaag tgacgatgac gccctgccgc tgaacagcct gctggccgcg
     3781 atctcacgtt tttcactgca aaccagcgcc cgtaaccagt ggttcggtac catcaccgcc
     3841 cgcgatcatg atgacattca acagcatgtt gatgtcttac tggctgacgg aaaaacacgc
     3901 ctgaaagtcg caattaccgc acaaagcggc gcacgtctgg ggctggatga aggcaaagaa
     3961 gtgttgatat tgctaaaagc gccgtgggta ggcattactc aggacgaagc ggtcgcacaa
     4021 aacgccgaca accaattacc gggtattatt agtcatattg agcgcggcgc agagcagtgc
     4081 gaagtattaa tggcgctacc cgacgggcaa acactgtgcg ccacagtgcc ggtaaatgaa
     4141 gcgacttctc ttcagcaagg gcagaatgtc acggcctact ttaatgccga cagcgtgatt
     4201 atcgccacgc tgtgctaagc gtgttgacaa tttgttatga aacacgtatc cctgtcagta
     4261 atcgctgcac aaaatgggat ataaaatgtc atcgttgcaa attttgcaag gcacgtttcg
     4321 tcttagcgac acaaaaacgc tgcaattgcc tcagctaacg ttaaacgcgg gtgatagttg
     4381 ggcgtttgtc ggttcgaatg gaagcgggaa atcggccctg gcccgcgcgc tggcggggga
     4441 acttccgctt ttgaaaggtg aacggcaaag ccagttttcc cacatcactc gtctctcctt
     4501 cgagcaattg caaaaactcg tcagcgacga atggcagcgg aataacaccg atatgctcgg
     4561 ccctggcgaa gatgacaccg gacgcactac ggctgaaatc attcaggatg aagtaaagga
     4621 tgcaccgcgt tgcatgcaac tggcgcagca gttcggtatt accgccctcc tcgaccgacg
     4681 ctttaaatac ctttccactg gcgagacgcg aaaaaccctg ctgtgtcagg cgctgatgtc
     4741 ggagcctgac ttgttgattc ttgatgagtc gttcgatggc ctggatgttg cctcacgtca
     4801 gcagctggct gagcgactcg cctcgttaca tcagtccggt attactctgg tactggtgct
     4861 caatcgcttc gatgagatcc cggagtttgt ccagtttgct ggcgtgctgg cggattgcac
     4921 gttagcggaa actggcgcta aagaggaact gctccaacaa gcactcgtcg cgcaactggc
     4981 gcatagtgaa cagcttgaag gtgtgcaact gccggagccg gatgaacctt cagcacgtca
     5041 cgccttaccc gccaacgaac cgcgcattgt gctgaacaat ggcgtggttt cttataacga
     5101 tcgccccatt cttaataacc ttagctggca ggtgaatcca ggtgaacact ggcaaattgt
     5161 cgggccaaat ggtgcaggaa aatcgacgtt attaagcctg gttactggcg atcatccgca
     5221 aggttacagc aacgatttga cgcttttcgg acgacgtcgc ggcagcggcg aaaccatctg
     5281 ggatatcaaa aagcatatcg gttacgtcag cagtagtttg catctggatt accgggtcag
     5341 cactaccgtg cgtaatgtga ttctttctgg ctattttgat tcgattggca tttatcaggc
     5401 cgtttcggat cgccagcaaa aactggtgca gcagtggctg gatattctcg gcattgataa
     5461 acgtacggct gacgctccgt tccatagtct ttcctgggga cagcagcgtc tggcgctgat
     5521 cgtccgcgca ctggtgaaac atccgacgtt gcttattctc gatgaaccac tacaggggct
     5581 tgatccgctc aatcgccagc ttatccgccg ttttgttgat gtgctgatta gcgaaggtga
     5641 aacgcaattg ttatttgttt cgcaccacgc tgaagatgcg cctgcctgta ttacccatcg
     5701 ccttgagttc gtgccggacg gtggactcta tcgctatgtg ctgacaaaaa tatattgagt
     5761 cggtagtgct gaccttgccg gaggcggcct aagcgccctc tccggccaac ggttcgacgc
     5821 atgcaggcat gaaaccgctt cttttttcag ataaaaagcg caatcattca taaaccctct
     5881 gttttataat cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa acgattccac
     5941 taatttattc catgtcacac ttttcgcatc tttgttatgc tatggttatt tcataccata
     6001 agcctaatgg agcgaattat gagagttctg gttaccggtg gtagcggtta cattggaagt
     6061 catacctgcg tgcaattact gcaaaacggt catgatgtca tcattcttga taacctctgt
     6121 aacagtaagc gcagcgtact gcctgttatc gagcgcttag gcggcaaaca tccaacgttt
     6181 gttgaaggcg atattcgtaa cgaagcgttg atgaccgaga tactgcacga tcacgctatc
     6241 gacaccgtga tccacttcgc cgggctgaaa gccgtcggcg aatcggtaca aaaaccgctg
     6301 gaatattacg acaacaatgt caacggcact ctgcgcctga ttagcgccat gcgcgccgct
     6361 aacgtcaaaa actttatttt tagctcctcc gccaccgttt atggcgatca gcccaaaatt
     6421 ccatacgttg aaagcttccc gaccggcaca ccgcaaagcc cttatggcaa aagcaaattg
     6481 atggtggaac agatcctcac cgacctgcaa aaagcccagc cggactggag cattgccctg
     6541 ctgcgctact tcaacccggt tggcgcgcat ccgtcgggcg atatgggcga agatccgcaa
     6601 ggcattccga ataacctgat gccatacatc gcccaggttg ctgtaggccg tcgcgactcg
     6661 ctggcgattt ttggtaacga ttatccgacc gaagatggta ctggcgtacg cgattacatc
     6721 cacgtaatgg atctggcgga cggtcacgtc gtggcgatgg aaaaactggc gaacaagcca
     6781 ggcgtacaca tctacaacct cggtgctggc gtaggcagca gcgtgctgga cgtggttaat
     6841 gccttcagca aagcctgcgg caaaccggtt aactatcatt ttgcaccgcg tcgcgaaggc
     6901 gaccttccgg cctactgggc ggacgccagc aaagccgacc gtgaactgaa ctggcgcgta
     6961 acgcgcacac tcgatgaaat ggcgcaggac acctggcact ggcagtcacg ccatccacag
     7021 ggatatcccg attaaggaac gaccatgacg caatttaatc ccgttgatca tccacatcgc
     7081 cgctacaacc cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc taagcgcccc
     7141 tggcaggggg cgcaggaaac gccagccaaa caggtgttac ctgcgcacga tccagattgc
     7201 ttcctctgcg caggtaatgt gcgggtgaca ggcgataaaa accccgatta caccgggact
     7261 tacgttttca ctaatgactt tgcggccttg atgtctgaca cgccagatgc gccagaaagc
     7321 aacgatcctt tgatgcgttg ccagagcgcg cgcggtacca gccgggtgat ctgcttttca
     7381 ccggatcaca gtaaaacgtt gccagaactg agcgttgcgg cattgacgga aatcgtcaaa
     7441 acctggcagg agcaaaccgc agagctggga aaaacgtacc cgtgggtgca ggtctttgaa
     7501 aacaaaggtg cggcgatggg ctgctctaac ccgcatccgc acggacagat ttgggcaaat
     7561 agcttcctgc ctaacgaagc tgagcgcgaa gaccgcctgc aaaaagaata ttttgccgaa
     7621 cagaaatcac caatgctggt ggattatgtt cagcgcgagc tggcagacgg tagccgtacc
     7681 gttgtcgaaa ccgaacactg gttagccgtt gtgccttact gggctgcctg gccgttcgaa
     7741 acgctactgc tgcccaaagc ccacgttttg cggatcaccg atttgaccga cgcccagcgc
     7801 agcgatttgg cgctggcgtt gaaaaagctg accagtcgtt atgacaacct cttccagtgc
     7861 tccttcccct actctatggg ctggcacggc gcaccattta atggcgaaga gaatcaacac
     7921 tggcagctgc acgcgcactt ttatccgcct ctgctgcgct ccgccaccgt acgtaaattt
     7981 atggttggtt atgaaatgct ggcagagacc cagcgagacc tgaccgcaga acaggcagca
     8041 gagcgtttgc gcgcagtcag cgatatccat tttcgcgaat ccggagtgta agaaatgagt
     8101 ctgaaagaaa aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc
     8161 attcaggcgc ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc
     8221 gttctgccct gcgcgattga ttatcaaacc gtgatcagct gtgcaccacg cgatgaccgt
     8281 aaagttcgcg tgatggcagc cgattatgaa aatcagctcg acgagttttc cctcaatgcg
     8341 cccattgtcg cgcatgaaaa ctatcaatgg gcgaactacg ttcgtggcgt ggtgaaacat
     8401 ctgcaactgc gtaacaacag cttcggcggt gtggacatgg tgatcagcgg caatgtgccg
     8461 cagggtgccg ggttaagttc ttccgcttca ctggaagtcg cggtcggaac cgtattgcag
     8521 cagctttatc atctgccgct ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa
     8581 aaccagtttg ttggctgtaa ctgcgggatc atggatcagc taatttccgc actcggcaag
     8641 aaagatcatg ccttgctgat tgactgccgc tcactgggga ctaaagcagt ttccatgccg
     8701 aaaggtgtgg ctgtcgtcat catcaacagt aacttcaaac gtaccctggt tggcagcgaa
     8761 tacaacaccc gtcgtgaaca gtgcgaaacc ggtgcgcgtt tcttccagca gccagctctg
     8821 cgcgatgtca ccattgaaga gttcaacgct gttgcacatg agctggaccc aatcgtggcg
     8881 aaacgcgtgc gtcatatcct gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg
     8941 gagcaaggcg atctgaaacg tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc
     9001 gatgatttcg aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagcagtg
     9061 attggcgaca aaggtggcgt acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg
     9121 ctgatcccgg aagagctggt gcctgccgta cagcaagctg tcgctgaaca atatgaagca
     9181 aaaacaggta ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc
     9241 tgaacgaaac tcccgcactg gcacccgatg gtcagccgta ccgactgtta actttgcgta
     9301 acaacgcagg gatggtagtc acgctgatgg actggggtgc gactttactt tcagctcgta
     9361 ttccgctttc cgatggcagc gtccgcgagg cgctgctcgg ctgtgccagc ccggaatgct
     9421 atcaggatca ggccgcgttt ctgggggcct ctattggtcg ttatgccaac cgcatcgcca
     9481 atagccgtta tacctttgac ggtgaaacgg tgacgctttc gccaagtcag ggcgttaacc
     9541 agctgcacgg cggcccggaa gggttcgaca aacgtcgctg gcagattgtg aaccagaacg
     9601 atcgtcaggt gctgtttgcc ctgagttcag atgatggcga tcagggcttc ccgggtaatc
     9661 tcggcgcgac ggtgcaatat cgtctgaccg acgataaccg tatctccatt acttatcgcg
     9721 ccacagttga taaatcttgc ccggtgaata tgactaatca cgtctatttc aatcttgacg
     9781 gcgagcagtc tgacgtgcgc aatcacaagt tgcagattct ggcgggcgaa tatctgccgg
     9841 ttgatgaagg cggcattccg cacgacggcc tgaaatctgt cgccggaacc tcttttgatt
     9901 tccgcagcgc caaaatcatc gccagtgagt ttcttgccga cgacgatcag cgcaaagtga
     9961 aaggttacga tcacgcattc ttgttacagg ccaaaggcga tggcaagaaa gtggcggcgc
    10021 atgtctggtc agcagatgaa aaattgcagc tgaaggtcta caccaccgct cccgctcttc
    10081 aattctactc cggcaacttc ctcggcggca caccgtcgcg gggaaccgaa ccttacgccg
    10141 actggcaagg actggcgttg gaaagcgagt ttctgccgga cagcccgaac caccctgaat
    10201 ggccgcaacc ggactgcttc ctgcgtcctg gcgaagagta ttccagcctg acggaatatc
    10261 agtttattgc tgagtaatgt taagccctcg tcatgagggc tttatcttca tattgttcaa
    10321 atcaccagca aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgcc
    10381 actattttcg ctatggttat gcgtaagcat tgctgttgct tcgtcacggc aatataatga
    10441 gaattattat cataaaaaga taatttgagg agtaagtata tggctgtaac taagctggtt
    10501 ctggttcgtc atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac
    10561 gtggatctgt ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag
    10621 gaaggttaca gctttgactt tgcttacact tctgtgctga aacgcgctat ccataccctg
    10681 tggaatgtgc tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg
    10741 aacgaacgtc actacggtgc gttgcagggt ctgaacaaag cggaaaccgc cgaaaagtat
    10801 ggcgacgagc aggtgaaaca atggcgtcgt ggttttgccg tgactccgcc ggaactgact
    10861 aaagatgatg agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgagaaagag
    10921 ctgccgctga ctgaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa
    10981 actattctac cgcgtatgaa gagcggtgag cgcgtgatca tcgctgcaca cggtaactct
    11041 ttacgtgcgc tggtgaaata tcttgataac atgagtgaag aagagattct tgagctgaat
    11101 atcccgaccg gcgtgccgct ggtgtatgag ttcgacgaga atttcaaacc gctgaaacgc
    11161 tattatctgg gtaatgctga cgagatcgca gcgaaagcag cggcggttgc taaccagggt
    11221 aaagcgaagt aaacgtcatt cgtttaaaaa tgagaaagcc gactgcaagt gagtcggctt
    11281 ttttgttgct aacaatggag cacattgcct gatgcgacgc tgcgcgtctt atcaggcctg
    11341 tggtgattca tcggatacgc cactctgacg gcgcatccga caattaaacc ttacccgcga
    11401 cgcgctttta ctgcattcgc cagttgacgt aacagagcat cggtatcttc ccagccaatg
    11461 caggcatcgg tgatgctctt accgtaggcc agcggctccc cgctatcgag gctctgattg
    11521 ccttccacca gatggctttc caccatcacg ccaataatgg ccttttcgcc accggcaatc
    11581 tgctggcaaa cgtcagcaca aacatccatc tgctttttga attgtttgga cgagttagca
    11641 tggctgaaat cgatcatcac ctgtgctggc aggcctgctt tgttcagccc ttctttcact
    11701 tcagcaacgt gcttcgcgct gtagttaggc tctttaccgc cgcgcagaat gatatggcaa
    11761 tcaccgttac cgctggtatt cacaatcgcc gaatgccccc atttagttac ggacaggaag
    11821 cagtgcggcg caccggcggc attaatggca tcgatagcca ctttaatcgt accgtctgtg
    11881 ccatttttaa aacctaccgg acaagaaaga ccagacgcca gttcgcggtg cacctgcgat
    11941 tccgtggtac gtgcaccaat tgcgccccag ctcatcaggt cagcgagata ctgaggagtg
    12001 atcatatcga gaaactcacc cgccgctggc agaccgctgt cgttaatatc aagcagcaat
    12061 ttacgggcta tacgcagacc gtcgttgatc tggaagctgt tatccatatg cggatcgtta
    12121 atcaaccctt tccagcccac cgtggtacgc ggcttttcaa aatagacgcg cattacgatt
    12181 tccagctcat ctttcagctc ttcacgcagc gccagcaagc gagtggcata ctctttagcc
    12241 gcgacaggat catgaattga gcacgggcca atcaccacca acaggcgatc atcattacct
    12301 ttcaggatct tatggatcgc ttttcgggca tgggcaaccg tattcgcggc attttcagtt
    12361 acggggaatt tttccagcaa tgcgacagga ggaagtaact ctttgatttc tttgatgcgt
    12421 aaatcgtcgt tctgataatt catgtctgtt ccagtgttgc catacttatc ttagtgaatg
    12481 caatacttcc aatctatatc ttccgtcaga atgtgtaaac ggggttttac actatgaacg
    12541 gattaatcct ggaattagca agaaaaacgc cagattgtcg cgaaaaacga gatctctcct
    12601 acaatttcta actgtaactc ctttgtttgt taattatttc aagattctct gctgcgtttc
    12661 ataaactggc tgaaatctta aaccaatgcc ttatattcac ctgcaaatgc actgttggaa
    12721 gaggttatcc gacataacga ccataacagg agcatcctat gaaaatgaca aaactggcca
    12781 cattatttct gactgccact ctaagccttg ccagcggtgc cgcactggct gccgatagcg
    12841 gagcgcaaac taataacggc caggcaaacg ccgcagctga tgcgggccag gtagcccctg
    12901 acgcccgtga aaatgtcgcg ccaaataacg tcgacaataa cggggtaaat accggttctg
    12961 gcggcacaat gctgcattcg gatggttctt caatgaacaa tgacggaatg accaaagatg
    13021 aaaagcacaa gaacaccatg tgcaaagatg gtcgatgtcc ggatatcaat aaaaaggtac
    13081 aaatcggtga tggcatcaat aatgatgtcg ataccaaaac cgacggtacc acgcagtaac
    13141 cctgcaaaat agccaataat gggagagcgg agtctctccc ttttaatttt tcacttctaa
    13201 aagcagtatg atgtcataaa gttaagtgag aataattaag ggatgactcg tcatggcgca
    13261 ctcacactca cacacgtcct cacacctgcc agaagataat aatgctcgtc gcttgttgta
    13321 tgctttcggc gtgactgccg ggtttatgct ggtagaagtc gttggtggtt ttctttctgg
    13381 ttctctggca ttgctggccg atgcgggtca tatgttgacc gatactgccg ccctgctttt
    13441 cgccctactc gccgtgcaat tttcacgtcg tcctcccacc attcgccaca ctttcggttg
    13501 gctaagactg accacgctcg cggcgtttgt gaatgccatc gccctcgtgg taattaccat
    13561 tttgattgtc tgggaggcga tagaacgctt ccgcacgccg cgtccggtcg agggcggcat
    13621 gatgatggca attgccgtgg ctgggttgct ggcaaatata ctttctttct ggttacttca
    13681 tcacggcagc gaagagaaaa acctcaacgt acgagcagcg gcattgcatg tgctcgggga
    13741 cctgttgggt tcggttgggg ctattattgc cgcgttgatt attatctgga ccggctggac
    13801 acctgctgac cccattctct cgatagtggt gtcgcttctg gttctgcgta gcgcgtggcg
    13861 actgttgaaa gatagcgtga atgaattact tgaaggtgca ccggtatcgc tggatatcgc
    13921 tgaactgaag cgtcgtatgt gccgggaaat cccggaagtc cgcaatgtgc accatgtaca
    13981 tgtatggatg gtaggcgaga agccggtgat aacgctgcat gtgcaggtga tcccgccaca
    14041 cgatcacgat gccttgttgg atcagatcca acactacctg atggatcact atcagattga
    14101 gcacgccacc attcagatgg aatatcaacc ttgtcatggg ccagactgcc acctgaatga
    14161 aggggtgtct ggccattcac atcatcacca ttaatgggac agcgcgcgtg agcctctttc
    14221 acgtgcgctg ttgatccaca tccgactgcc gttgagcgca ataaaggtca ggatgatgta
    14281 ctccagagac atggcgtaaa cgccctgaag tgcaaaaata acgacgctaa tcacgttaat
    14341 aatcacccac aacagccagt tttccacata cttacgcgtc atcagaatca ttgccacgat
    14401 agataacacc atcatgcatg aatcccagaa cgggaaagcg tccggttgca gttcaggcat
    14461 cacaacctgt aatcctaatg cttgcatgat catgactgcc acgcgggtca aaaatgcaaa
    14521 caccggattg ataaataccg tcatcagacc aatcgaaaca acgcaaaccg ccaaccagct
    14581 gagcgccttc ggcaatggta accagcgaat tttcaactcc gcctcgttct gactggtttg
    14641 tcgcgaccac gcataccaac cgtaaatatt cgcggcaaag aaaaacacct gtaatagcag
    14701 gctggcatac agctgaatct gaaagaaaat aatgccaaac aaggtgacgt tgatcaggcc
    14761 aaagaagtag ttgctgatct tctccagact ggcaaggcca atacacagca acccggcgat
    14821 cgtgcctacc gcttcgatcc atgagagatc ataaccgcct gcccctattg gtatatgtac
    14881 caggatattc tgcacactaa aaaaatccat ctttccccca aagcgtaacg ttacgcctta
    14941 ttattcgtta tccacgtagt gtagccgcaa aatccagcat acgattgagc ggcaccagcg
    15001 ccctctctcg cagcctttca tcaagaacct cgtgattgct tccttcctgt tctaatgcct
    15061 ctgcgatgga ctgaaggcca ttcatggcca tccacggaca atgcgcgcag
//
LOCUS       DAJVYM010000095        15084 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVYM010000095 DAJVYM010000000
VERSION     DAJVYM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.096"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..781
                     /locus_tag="OQS60_002706"
     CDS             <1..781
                     /locus_tag="OQS60_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388415.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OQS60_002707"
     CDS             complement(984..1337)
                     /locus_tag="OQS60_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS2388416.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OQS60_002708"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OQS60_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS2388417.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OQS60_002709"
     CDS             complement(3165..3458)
                     /locus_tag="OQS60_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS2388418.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OQS60_002710"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OQS60_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS2388419.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OQS60_002711"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OQS60_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS2388420.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OQS60_002712"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OQS60_002712"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS2388421.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OQS60_002713"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OQS60_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS2388422.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OQS60_002714"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OQS60_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS2388423.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OQS60_002715"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OQS60_002715"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS2388424.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OQS60_002716"
     CDS             10838..11413
                     /locus_tag="OQS60_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2388425.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OQS60_002717"
     tRNA            11520..11595
                     /locus_tag="OQS60_002717"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OQS60_002718"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OQS60_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="OQS60_002719"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="OQS60_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS2388426.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAJVYM010000096        14841 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVYM010000096 DAJVYM010000000
VERSION     DAJVYM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.097"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..2688
                     /locus_tag="OQS60_002720"
     CDS             <1..2688
                     /locus_tag="OQS60_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCS2388427.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSA
                     KHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAP
                     TYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANG
                     AISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALD
                     ALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMM
                     IEQNTQIINQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVA
                     SGASSVAIGQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGVVIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLA
                     VGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQT
                     TRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDER
                     VAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSV
                     AIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLK
                     SSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSVQE
                     TKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGES
                     AVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3056..4711
                     /gene="lldP"
                     /locus_tag="OQS60_002721"
     CDS             3056..4711
                     /gene="lldP"
                     /locus_tag="OQS60_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS2388428.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4711..5487
                     /gene="lldR"
                     /locus_tag="OQS60_002722"
     CDS             4711..5487
                     /gene="lldR"
                     /locus_tag="OQS60_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS2388429.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            5484..6674
                     /gene="lldD"
                     /locus_tag="OQS60_002723"
     CDS             5484..6674
                     /gene="lldD"
                     /locus_tag="OQS60_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS2388430.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            6870..7343
                     /gene="trmL"
                     /locus_tag="OQS60_002724"
     CDS             6870..7343
                     /gene="trmL"
                     /locus_tag="OQS60_002724"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS2388431.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7396..8217)
                     /gene="cysE"
                     /locus_tag="OQS60_002725"
     CDS             complement(7396..8217)
                     /gene="cysE"
                     /locus_tag="OQS60_002725"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS2388432.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8297..9316)
                     /gene="gpsA"
                     /locus_tag="OQS60_002726"
     CDS             complement(8297..9316)
                     /gene="gpsA"
                     /locus_tag="OQS60_002726"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS2388433.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9316..9783)
                     /gene="secB"
                     /locus_tag="OQS60_002727"
     CDS             complement(9316..9783)
                     /gene="secB"
                     /locus_tag="OQS60_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS2388434.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9846..10097)
                     /gene="grxC"
                     /locus_tag="OQS60_002728"
     CDS             complement(9846..10097)
                     /gene="grxC"
                     /locus_tag="OQS60_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS2388435.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10239..10670)
                     /locus_tag="OQS60_002729"
     CDS             complement(10239..10670)
                     /locus_tag="OQS60_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS2388436.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10915..12459
                     /gene="gpmM"
                     /locus_tag="OQS60_002730"
                     /gene_synonym="pgmI"
     CDS             10915..12459
                     /gene="gpmM"
                     /locus_tag="OQS60_002730"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS2388437.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12469..13752
                     /gene="envC"
                     /locus_tag="OQS60_002731"
     CDS             12469..13752
                     /gene="envC"
                     /locus_tag="OQS60_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS2388438.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13756..14715
                     /locus_tag="OQS60_002732"
     CDS             13756..14715
                     /locus_tag="OQS60_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS2388439.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AVPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         3538 a         3954 c         3962 g         3387 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac
      241 tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc
      301 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
      361 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
      421 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
      481 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
      541 accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
      601 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
      661 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
      721 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
      781 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
      841 aacgccctgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat
      901 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
      961 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     1021 attatcaatc agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt
     1081 atcaactacg ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt
     1141 gtcggcgcta cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt
     1201 ggtcagaaca gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc
     1261 agccgtgtga ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt
     1321 ggttatgaca ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa
     1381 taccgccaaa tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc
     1441 cagttgcaga acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac
     1501 tcaacggcag aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact
     1561 gtcgttaatg gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg
     1621 atcaacggca ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg
     1681 ggtaacggtt ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac
     1741 gcaccgcaga actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc
     1801 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     1861 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     1921 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     1981 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     2041 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     2101 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     2161 atcaccaacg tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     2221 tcttctgaag caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc
     2281 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     2341 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag
     2401 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     2461 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     2521 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     2581 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     2641 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     2701 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     2761 aggcgccccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     2821 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     2881 cacaattccc caattaaaac tcacatcaat attgccaata cataacattt agttaaccat
     2941 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     3001 aggagatgag caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa
     3061 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     3121 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     3181 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     3241 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     3301 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     3361 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     3421 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     3481 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     3541 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     3601 agtaacaggc atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat
     3661 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga
     3721 gacgtggcct gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc
     3781 taacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     3841 gacgctgttc ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc
     3901 gtcaaagatt gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc
     3961 ctggacaccg ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa
     4021 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     4081 cctcgataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     4141 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     4201 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     4261 gaaagaactg gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     4321 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     4381 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     4441 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     4501 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     4561 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     4621 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     4681 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     4741 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     4801 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag
     4861 gcgctggcaa aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     4921 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     4981 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     5041 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     5101 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     5161 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     5221 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     5281 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     5341 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     5401 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     5461 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     5521 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     5581 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     5641 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     5701 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     5761 ccaaagcggc ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     5821 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     5881 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     5941 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     6001 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     6061 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     6121 aaccgaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     6181 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     6241 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     6301 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     6361 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     6421 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     6481 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     6541 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     6601 attcgctggt gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag
     6661 ggaatgcggc atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga
     6721 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     6781 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     6841 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     6901 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata
     6961 tcatcgaacc gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact
     7021 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
     7081 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
     7141 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
     7201 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
     7261 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
     7321 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
     7381 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
     7441 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
     7501 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
     7561 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
     7621 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
     7681 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
     7741 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
     7801 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
     7861 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
     7921 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
     7981 ggcgatcatt tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc
     8041 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt
     8101 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
     8161 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
     8221 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
     8281 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg
     8341 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
     8401 ttggcatttc tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
     8461 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
     8521 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
     8581 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca
     8641 tttcagccag cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt
     8701 cggacatccc cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga
     8761 tgaaatccgg gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat
     8821 catcggcaaa ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca
     8881 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
     8941 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
     9001 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
     9061 ggctgggtac gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt
     9121 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
     9181 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
     9241 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
     9301 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
     9361 caaatagttc atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt
     9421 accgcgggat accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
     9481 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
     9541 gccctgctga acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac
     9601 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
     9661 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
     9721 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
     9781 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
     9841 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
     9901 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
     9961 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg
    10021 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    10081 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    10141 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    10201 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    10261 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    10321 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    10381 accggtttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    10441 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    10501 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    10561 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    10621 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    10681 aactcccgac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    10741 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    10801 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    10861 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    10921 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    10981 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    11041 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    11101 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    11161 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg
    11221 gtagataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    11281 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    11341 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    11401 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    11461 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    11521 gcctatgacc tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt
    11581 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    11641 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    11701 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    11761 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    11821 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    11881 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    11941 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    12001 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    12061 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    12121 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    12181 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    12241 gctgaccacg gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca
    12301 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    12361 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    12421 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    12481 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    12541 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    12601 cgtgaccaac tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa
    12661 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    12721 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    12781 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    12841 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    12901 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    12961 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag
    13021 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    13081 caacaggcga aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa
    13141 tcttccatcc agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg
    13201 cgtaacagta ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    13261 gaggctcagg cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa
    13321 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    13381 caggcattct ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt
    13441 gaattacgct ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    13501 gccgacggtc gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt
    13561 gagcacggta aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt
    13621 ggttcgcagg ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc
    13681 agaccttcac tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg
    13741 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    13801 gcgctctcct cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    13861 cgcccgaaca acgaaaacca ggtgctggcg gtgccttccg ctatctccgt cgctgtatta
    13921 cccgattcac cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    13981 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc
    14041 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    14101 tatgccgtgg ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg
    14161 cagaaagtga tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt
    14221 ggtaataccc aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag
    14281 gtgttcctcg acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt
    14341 gacctagcgc gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta
    14401 cgcgtgttgc aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    14461 agcttgctga atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac
    14521 acaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    14581 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    14641 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac
    14701 gctagcgtaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    14761 attgccataa gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat
    14821 tggtaatcag gcgcttatac a
//
LOCUS       DAJVYM010000097        14744 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVYM010000097 DAJVYM010000000
VERSION     DAJVYM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.098"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(226..1089)
                     /locus_tag="OQS60_002733"
     CDS             complement(226..1089)
                     /locus_tag="OQS60_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS2388440.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(1061..2611)
                     /locus_tag="OQS60_002734"
     CDS             complement(1061..2611)
                     /locus_tag="OQS60_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS2388441.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2869..3648
                     /gene="deoC"
                     /locus_tag="OQS60_002735"
     CDS             2869..3648
                     /gene="deoC"
                     /locus_tag="OQS60_002735"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS2388442.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3775..5097
                     /gene="deoA"
                     /locus_tag="OQS60_002736"
     CDS             3775..5097
                     /gene="deoA"
                     /locus_tag="OQS60_002736"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS2388443.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5149..6372
                     /gene="deoB"
                     /locus_tag="OQS60_002737"
     CDS             5149..6372
                     /gene="deoB"
                     /locus_tag="OQS60_002737"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS2388444.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6452..7171
                     /gene="deoD"
                     /locus_tag="OQS60_002738"
     CDS             6452..7171
                     /gene="deoD"
                     /locus_tag="OQS60_002738"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS2388445.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7446..7595)
                     /locus_tag="OQS60_002739"
     CDS             complement(7446..7595)
                     /locus_tag="OQS60_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388446.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(7627..8643)
                     /gene="lplA"
                     /locus_tag="OQS60_002740"
     CDS             complement(7627..8643)
                     /gene="lplA"
                     /locus_tag="OQS60_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS2388447.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(8671..9315)
                     /locus_tag="OQS60_002741"
     CDS             complement(8671..9315)
                     /locus_tag="OQS60_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS2388448.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            9421..10389
                     /gene="serB"
                     /locus_tag="OQS60_002742"
     CDS             9421..10389
                     /gene="serB"
                     /locus_tag="OQS60_002742"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS2388449.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10438..11820
                     /gene="radA"
                     /locus_tag="OQS60_002743"
     CDS             10438..11820
                     /gene="radA"
                     /locus_tag="OQS60_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS2388450.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            11841..13073
                     /gene="nadR"
                     /locus_tag="OQS60_002744"
     CDS             11841..13073
                     /gene="nadR"
                     /locus_tag="OQS60_002744"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS2388451.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13380..>14744)
                     /gene="ettA"
                     /locus_tag="OQS60_002745"
     CDS             complement(13380..>14744)
                     /gene="ettA"
                     /locus_tag="OQS60_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS2388452.1"
                     /translation="KRLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLE
                     RAADALRLPDWDAKIANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLER
                     FLHDFEGTVVAITHDRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQ
                     EAARRKSIEKELEWVRQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRL
                     GDKVLEVSNLRKSYGDRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDS
                     GTITLGETVKLASVDQFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKG
                     VDQGKRVGELSGGERGRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGC
                     AMVISHDRWFLDRIATHILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYK
                     RIAK"
BASE COUNT         3358 a         3869 c         4075 g         3442 t
ORIGIN      
        1 gaaccgtagg ccggataagg cgttcacgcc gcatccggca gttggcgcac aatgcctgat
       61 gcgacgctga cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg
      121 cgttcacgcc gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta
      181 tcatgcctac aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc
      241 cggaaatatc aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc
      301 ttccggcgtg gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc
      361 attcaggcga accggaacat cgccaagtct cttgataaac gtcgccagct catcgatgtg
      421 atgcaaataa tccacctggt ctggaattac cagcaaacgc agttccgcca gcttgccgcg
      481 ctctgcaagc aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaagtgttg
      541 atgacattcg ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa
      601 tttttcccag ccggtttcgc tcaacatgcc gttactgtcc accaggcagg tgaggtggcg
      661 cagttgcgga tcgtttttga tagcagtaaa cagcgccact acaaacggca gttgggtcgt
      721 ggcttcgccg ccgcttaccg ttatcccttc aataaacagc accgctttgc ggacatggct
      781 aagcacttcg tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacctctt
      841 caggcaggta tcacactgct cgcaaaccac agcgttccac accactttgc cgtcaacaat
      901 ctgcaacgcc tgatgcggac actgtggcac gcactcccca cagtcattgc aacgtcccat
      961 cgtccacgga ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag
     1021 acgactgcct ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct
     1081 gctgttcatg gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt
     1141 cttcgcccag ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg
     1201 acaaacgcac catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcagtaa
     1261 attcacgcat tccggcttta aaggcaccga ggcaaagctg taccagcgcc tgcggattac
     1321 gtttgatggt ttcgtcgagc gtcagaatgt cgctgatgcc ggaataataa taagcatgat
     1381 gcggggcgac agtttgcaga tgggtgatcg gatctggctc atcaccatac ggcaaacgcg
     1441 cgcccggcgt ggtgccgata tcggaactgc tccccgactg tgcgtgtaac atggcgcgtt
     1501 tttgccagcc atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc
     1561 gataacccac ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct
     1621 tacacagcaa gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt
     1681 cagggttaat cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat
     1741 agaggaattc acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag
     1801 tgcgcgtaaa gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc
     1861 gtaccagcgt gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt
     1921 agcccccttt tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac
     1981 attcacagat gttcttcgcc acttccagca gcaggtcatc aggggtgatt tcaggatcgt
     2041 agataaaggt caggttcggt gaaacctgct tcaactctgc gtctgcacgt aagatcgcgc
     2101 gggtaatggg cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc
     2161 tgtcgaggta acgccagaaa cgttttattc gaacatcgat ctcgtcttgt gttagaattc
     2221 taacatatgg ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg
     2281 acggtacgtg gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc
     2341 cttccagctc cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat
     2401 agcgcggttt gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg
     2461 cgcggcgggc ttcggcagga agctgcggat aaggcagatt gttttctgct tccagtgcca
     2521 gaaaatggcg cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt
     2581 gttgcagtgc attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg
     2641 cgccattgta agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac
     2701 gcattcattt gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa
     2761 gtagatttcc ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact
     2821 aacaaactcg caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg
     2881 aaagcaagca gcctgcgtgc actgaaattg atggacctga ccaccctgaa tgacgacgac
     2941 accgacgaga aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc
     3001 gctatctgta tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc
     3061 accccggaaa tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa
     3121 atcgcgctgg cagaaacccg tgcggcaatc gcctacggtg ctgatgaagt tgacgttgtg
     3181 ttcccgtacc gcgcgctgat ggcgggtaac gagcaggttg gttttgacct ggtgaaagcc
     3241 tgtaaagagg cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa
     3301 ctgaaagacg aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac
     3361 ttcatcaaaa cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc
     3421 atgatggaag tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accagcgggc
     3481 ggcgtgcgta ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgttcggt
     3541 gctgactggg cagatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg
     3601 ttgaaagcgc tgggccacgg tgacggtaag agcgccagca gctactaagt aagatgcttt
     3661 acgcctgatg cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag
     3721 gcgtagacgc atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt
     3781 ctcgcacaag aaattattcg taaaaaacgt gatggtcatg cactgagcga tgaagaaatt
     3841 cgtttcttta tcaacggcat tcgcgacaac actatctccg aagggcagat tgccgccctc
     3901 gcgatgacca ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg
     3961 atgcgagatt caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt
     4021 gataaacatt ccactggtgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc
     4081 gcagcctgcg gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt
     4141 acgctcgaca aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc
     4201 cgcgaaatta ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg
     4261 gcggataaac gtttctacgc gacccgtgat attaccgcaa ccgtggactc catcccgctg
     4321 atcaccgcct ctattctggc gaagaaactt gcggaaggtc tggacgcgct ggtgatggac
     4381 gtgaaagtgg gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa
     4441 gcgattgttg gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg
     4501 aatcaggtac tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc
     4561 ctgacgggtg agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag
     4621 atgcttatct ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaagca
     4681 gtgctggaca acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc
     4741 ccaaccgact tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca
     4801 gtctatgctg ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca
     4861 gtggttgcaa tgggcggcgg tcgtcgtcag gcatctgaca ccattgatta cagcgtcggc
     4921 tttactgata tggcgcgttt gggcgaccag gtagacggtc agcgtccgct ggcggttatc
     4981 cacgcgaaag acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa
     5041 cttgccgata aagcaccgga aagcacacca actgtctatc gccgtatcag cgaataacgg
     5101 tatactgatc tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca
     5161 tttattatgg tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt
     5221 gacgtcgggg ctgacaccct gggtcatatc gcagaagctt gtgccaaagg cgaagctgat
     5281 aacggtcgta aaggtccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca
     5341 cacgaaggtt ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc
     5401 gcgtacgcat gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa
     5461 atagccggtg tcccggttct atttgagtgg ggatatttct ccgatcacga aaacagcttc
     5521 ccgcaagagc tgctggataa actggttgaa cgcgctaatc tgccgggtta cctcggtaac
     5581 tgccactctt ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc
     5641 aagccgattt tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc
     5701 tttggcctgg ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc
     5761 ggctacaaca tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc
     5821 cagcgtaccg gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag
     5881 aaactggttg atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc agacatctac
     5941 gccaactgcg gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc
     6001 accatcaaag agatgaaaga agcgggtgat aacaccatcg tcttcaccaa cttcgttgac
     6061 ttcgactctt cctggggcca ccgtcgcgac gtcgccggtt atgctgtggg tctggagctg
     6121 ttcgaccgcc gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc
     6181 actgctgacc acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt
     6241 ccggtactgg tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc
     6301 gcggatatcg gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa
     6361 gccatgttct gataatgggt gcggcttgat gccctcaccc cggccctctc cctcagggag
     6421 agggtgaaaa catgttgata aggataaaac aatggctacc ccacacatta atgcagaaat
     6481 gggcgatttc gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcga agtatattgc
     6541 tgaaactttc cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac
     6601 cggtacttac aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg
     6661 ctccatctac accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg
     6721 ttcctgtggc gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc
     6781 ctgcaccgat tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc
     6841 tgactttgac atggtgcgta acgcggtaga cgcggctaaa gcactgggca ttgatgctcg
     6901 cgttggtaac ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt
     6961 gatggaaaaa tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtcgc
     7021 tgcagaattt ggcgcgaaag ccctgaccat ctgcactgtg tctgaccaca tccgcactca
     7081 cgagcagacc actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact
     7141 ggaatccgtt ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gattgtcgtg
     7201 ttaagccgga gtgggggact gctccggctt tttagtatct attcatcaat agcaactgat
     7261 ttatagattt actattattt ttcatatgga taaaaaataa aatacattca agtatctatt
     7321 cacgaatcta ttcatttatg ttcagccgtc atccaggaag ggcatatcag caactctggt
     7381 ggacaaccat aaatttctcc cattttgctt ttgatgtttg agctaagccg ttccgtcttt
     7441 tcgatctatt ttagagaatt aatcgagata cctaacattt tacttacttc agcaagtgat
     7501 aatttatgcc tgatacgcac ggcggctagc caggaaatac gctggcttaa cattaaattg
     7561 ataatctccc gctcctggtt ttccagatcg atcatattcc cttcctttgg tattttaatc
     7621 agcgagttac cttaccgccc ccgctatcca cgtcgacaac tcccgtagct ctttttcctg
     7681 gtccgggaag tcaaccaaca gcgcttcgca ctcttgttgc agcatatccg cgcggtacag
     7741 gcagccttgc agtcgcccgg cgagggcttc cagcggcgcg gggttgaggc tgtcggtaaa
     7801 cacctgggcg cgggtgatat ggcctttttc aacgtcgaaa tgcagttcca cgccgcccca
     7861 gctaaagcgt tcatccagca gatgcgagaa tgccggagcc tgaccgaagt tccattccca
     7921 gctactctga cgggcaaagg tttcggcgaa gtttggcaag tctggcgttt tgtccgggga
     7981 gatgatttcc gcttctacac gctcgccata atgggcgaaa aaggccttgg ttatggcctc
     8041 gcaaacctgc tcatgggtga tccccggcag cagctcggtg aggttggtca cgcgggaacg
     8101 cactgaggta atgcctttcg ccgccagttt ctttttatcc ggattgagat agtttgccag
     8161 gcggctaagg tcggcattga gcagcaaagt gccgtggtgg aagccacgat ctttggtttc
     8221 gcgataggcc gagcctgaga ctttgcggtc gccttcgact gttttcacca ccagatcgtt
     8281 acgtccggac gcttcggcgc tgacgccgag cgcgttcagc gcattgagca caatcgacgt
     8341 agagatggtt ttatcgtact ccggcttgcc agccataaag gtaaagcagg tattgccgag
     8401 atcgtggaac accgcgccgc caccgctact gcgccgcgcc aggcggacgt tatcttcttc
     8461 catccgccgg gtattacact ctttccacgg gttctgcgcg cgaccaatca ctaccgtgtc
     8521 ggcattgcgc cagagaaaca gaacgcgctg cgtggcgggc atttggcgaa aaatacactc
     8581 ttccaccgcc aggttaaacc acgggtcgta agagtcagag atgagcaggc gtaatgtgga
     8641 cataacgatt tcctttcttg tagtaatggg tcactctttt ttctcgcttt cttcctcttc
     8701 cggcaccggt ttgctggcgg ttaacaggaa gggcgattgc tgccagcggg tgcgtttacc
     8761 ctgtagcagc gtgcgggtca gcactacgcc gattgccagt gagagcagca acatcaggcg
     8821 taaaatgtta gtggtgttat ccacctgttg ggcttcggtg gcgagcgtat gggtgtcgag
     8881 tgtcaggcgc agatagccga gcggtccgtt tttacccgca attggctcga caatctgctg
     8941 gttaaaatag ccgcctgctt ttttaccgtc gagcgccagc cggtcgcgca cttcgacgct
     9001 ttcgcctgaa cgggcgataa gatcgccttg ttcgtcatac acacccgcgt cgaggatgcg
     9061 gctttcatcc gttaactgat cgaggatcgc ctgaatgcgt ttttcatctg gtgagtcggt
     9121 acgcatcagc ggtgcaacgt tcagcgtcac ctgacgcgcc agggtgcggg ccagttcttc
     9181 cagctgtgga ttacgctgtc gctggtggtt ttgactaaac catgacgccc cctgcatcag
     9241 tgccactaac aaggcaagac agaacaggac aatcactgcc cgatgcagcc ggaatttcag
     9301 ttttgtgcga gccatcttcc accctttgaa aatttgagac ttaatgttgc cagaagcaat
     9361 ggatacaagg tagcctcatg cgttattttc cctgcttcga acgattttac aggagcctta
     9421 atgcctaaca ttacctggtg cgacctgcct gaagatgtct ctttatggcc gggtctgcct
     9481 ctttcattaa gtggtgatga agtgatgcca ctggattacc acgcaggtcg tagcggctgg
     9541 ctgctgtatg gtcgtgggct ggataaacag cgtctgaccc aataccagag caaactgggt
     9601 gcggcgatgg tgattgttgc cgcctggtgc gtggaagatt atcaggtgat tcgtctggca
     9661 ggttcactca ccgcacgggc tacgcgcctg gcccatgaag cgcaactgga tgtcgccccg
     9721 ctggggaaaa tcccgcacct gcgcacgccg ggtttgctgg tgatggacat ggattccacc
     9781 gccatccaga ttgaatgtat tgatgaaatt gccaaactgg ccggaacggg cgagatggtg
     9841 gcggaagtaa ccgaacgggc gatgcgcggc gaactcgatt ttaccgccag cctgcgcagc
     9901 cgtgtggcga cgctgaaagg cgctgacgcc aatattctgc aacaggtgcg tgaaaatctg
     9961 ccgctgatgc caggcttaac gcaactggtg ctcaagctgg aaacgctggg ctggaaagtg
    10021 gcgattgcct ccggcggctt tactttcttt gctgaatatc tgcgcgacaa gctgcgcctg
    10081 accgccgtgg tagccaatga actggagatc atggacggta aatttaccgg caatgtgatt
    10141 ggcgacatcg tagacgcgca gtacaaagcg aaaactttga ctcgcctcgc gcaggagtat
    10201 gaaatcccgc tggcgcagac cgtggcgatt ggcgatggag ctaatgacct gccgatgatc
    10261 aaagcagcag ggctggggat tgcctaccat gccaagccaa aagtgaatga aaaggcggaa
    10321 gtcaccatcc gtcacgctga cctgatgggg gtattctgca tcctctctgg cagcctgaat
    10381 cagaagtaat tgctcgcccg ccatcctgcg ggcggcacag cattaacgag gtacaccgtg
    10441 gcaaaagctc caaaacgcgc ctttgtttgt aatgaatgcg gggccgatta tccgcgctgg
    10501 caggggcagt gcagtgcctg tcatgcctgg aacaccatca ccgaggtgcg tcttgctgcg
    10561 tcgccaacgg tggcgcgtaa cgagcgtctc agcggctatg ccggtagcgc cggggtggca
    10621 aaagtccaga aactctccga tatcagcctt gaagagctgc cgcgtttttc caccggattt
    10681 aaagagttcg accgcgtact aggcggcggc gtggtgccgg gaagcgccat tctgattggc
    10741 ggtaacccag gtgcggggaa atccacgctg ctactgcaaa cgctgtgcaa actggcccag
    10801 cagatgaaaa cgctgtatgt cactggcgaa gagtcgctgc aacaggtggc aatgcgcgct
    10861 catcgccttg gcctgccgac tgacaatctc aatatgttgt cggaaaccag catcgaacag
    10921 atctgcctga ttgccgaaga agagcaaccg aaactgatgg taattgactc gatccaggtg
    10981 atgcatatgg cggatgtaca atcatcgcct ggtagcgtgg cgcaggtgcg tgaaacggcg
    11041 gcttatctga cgcgcttcgc caaaacgcgc ggtgtggcga ttgtcatggt tgggcacgta
    11101 accaaagatg gctcgctggc tggccctaaa gtgctggaac actgtatcga ctgttcggtg
    11161 cttctggatg gtgatgccga ctcccgtttt cgcaccttgc gcagccataa aaaccgcttc
    11221 ggcgcggtga atgagctggg cgtcttcgcg atgaccgaac aggggctgcg tgaagtcagc
    11281 aacccttcgg caattttctt aagtcgcgga gatgaagtga cctccggcag ctccgtgatg
    11341 gtggtgtggg aaggaacgcg tccgttgctg gtggagattc aggcgctggt cgatcactcg
    11401 atgatggcga atccgcgccg cgtggcagtg gggctggaac aaaaccgtct ggcaatcctg
    11461 ctggctgtct tgcaccgtca cggtggtctg caaatggccg atcaggatgt atttgtgaac
    11521 gtggtcggcg gcgtgaaggt aaccgaaacc agtgccgatt tagcgttact gctggcgatg
    11581 gtttccagcc tgcgtgacag accgctgcca caggatctgg tggtgtttgg tgaagtcggg
    11641 ctggcagggg agatccgccc ggtgcccagc ggtcaggaac gaatttcaga agcggcgaaa
    11701 cacggttttc gccgggcgat tgttccggcg gctaacgtac cgaaaaaagc gccggaaggg
    11761 atgcagattt ttggcgttaa aaaactctcc gacgcgctta gcgtgttcga cgacttataa
    11821 tgagagatac ggagggagat atgtcgtcat ttgattacct gaaaactgcc atcaagcaac
    11881 agggctgcac gctacagcag gtagctgatg ccagcggtat gaccaaaggg tatttaagcc
    11941 agttactgaa tgccaaaatt aaaagcccca gcgcgcaaaa gctggaggcg ttgcaccgtt
    12001 ttttggggct tgaatttccc cgacagaaga aaaccattgg tgtcgtattc ggtaagttct
    12061 acccgctgca taccggacat atctacctta tccagcgcgc ctgtagccag gttgacgagc
    12121 tgcatatcat tatgggtttt gacgataccc gcgaccgcgc gttgtttgaa gacagcgcca
    12181 tgtcgcagca gcccacggtg ccggatcgtc tgcgctggtt attacaaact tttaaatatc
    12241 agaaaaatat tcgcattcat gctttcaacg aagagggcat ggagccgtat ccgcacggct
    12301 gggatgtgtg gagcaacggc atcaaaaagt ttatggctga aaaggggatt cagccggacc
    12361 tgatctacac ctcggaagaa gccgatgcgc cacagtatat ggaacatctg gggatcgata
    12421 cggtgctggt cgatccgaaa cgtaccttta tgagtatcag cggtgcgcag atccgcgaaa
    12481 acccgttccg ctactgggaa tatattccta cggaagtgaa gccgttcttt gtacgtaccg
    12541 tggcgatcct tggtggcgag tcgagcggta aatccaccct ggtaaacaaa cttgccaata
    12601 tcttcaatac caccagtgcg tgggaatatg gtcgcgatta tgtcttttca cacctcggcg
    12661 gtgatgagat cgcattgcag tattccgatt acgataaaat cgcgctgggc cacgcacaat
    12721 acattgattt tgcagtgaaa tatgccaata aagtggcgtt tattgacact gattttgtca
    12781 ctacccaggc gttctgcaaa aagtacgaag ggcgtgagca tccgttcgta caggcgttga
    12841 ttgatgaata ccgtttcgat ctggtgatcc tgctggagaa caacacgccg tgggtggcgg
    12901 atggtttacg cagcctcggc agttcggtgg atcgcaaaga gttccagaac ttgctggtgg
    12961 agatgctgga agagaacaat atcgaattcg tgcgggttga agaggacgat tatgacagcc
    13021 gtttcctgcg ctgcgtggag ctggtgcggg agatgatggg ggagcagaga taaccgcgat
    13081 gaaacggctc aaaggcgagg tataaaataa gttttttgat gagttgaaaa cctctctgga
    13141 agaggctgtc gagattaaac aaggtttgaa aaaacctgca cgggtgaccc gctacgaaat
    13201 tgcggatact aaggctgttg taggccggaa aaggcgttca cgccgtatcc ggcattcggt
    13261 gctcaatgcc tgatgcgacg cttaccgcgt cttatcaggc ctggagattc acaacgtatt
    13321 gaaatttctt ggtttgtagg ccggataagg cgttcacgcc gcatccggca ttttacgcat
    13381 tacttcgcaa tacgcttgta cttgatacgc ttcggctcca gtgcgtctgc gcccagcgtg
    13441 cgtttcttgt actcttcgta ctcagtaaag ttaccttcga agaactcaac tttaccttca
    13501 tcctggtagt ccaggatgtg cgtggcgata cggtcgagga accaacggtc gtgcgagata
    13561 accatcgcac agcccgggaa ctccagcagg gcgttttcca gcgcgcgcag ggtttcgata
    13621 tccaggtcgt tggttggttc gtcgagcagc agcatgttgc cgccaacctg cagcagcttc
    13681 gccagatgca gacgaccgcg ctcaccaccg gaaagttcac caacgcgttt accctgatca
    13741 acccctttaa agttaaagcg gccaacgtag gcgcggcttg gcatctcggt gttgccgatc
    13801 ttcataatat ccagcccgcc ggaaacttct tcccaaacgg ttttgctgtt atccattgag
    13861 tcacggaact gatcaaccga cgccagtttc accgtttcac ctaaagtgat ggtgccgctg
    13921 tccggctgtt cctgaccaga gatcatacgg aacagggtcg atttacccgc gccgttcgga
    13981 ccgatgatcc cgacgattgc ccctttcggg atcgagaagc tcaggtcatc aatcagcagg
    14041 cgatcaccgt aggatttacg caggttgctg acttccagca ctttatcgcc cagacgcggt
    14101 ccaggtggaa taaacagttc gttggtttcg ttacgtttct gatattcggt gctgttcagc
    14161 tcttcaaagc gtgccagacg tgctttacct ttcgactggc ggcctttagt tccctgacgt
    14221 acccactcca gctctttctc gatcgactta cgacgcgccg cttcttgtga agcttcctgc
    14281 gccaggcgct gatctttctg ctccagccag gaggagtagt tgccttccca cggaatacct
    14341 tcaccgcggt caagttcgag gatccagcct gcaacgttat cgaggaagta acggtcgtgg
    14401 gtaatcgcca ccacggtgcc ctcgaagtcg tgcaggaagc gttccagcca ggccacggat
    14461 tcggcatcca ggtggttggt cggttcgtcg agcagcagca tgtctggttt ttccagcagc
    14521 aggcggcaca acgctacgcg acgacgctca ccaccggaga ggttagcgat tttcgcgtcc
    14581 cagtccggca gacgtagcgc atccgccgca cgctccagct gtacgttcag gttatgaccg
    14641 tcgtgagcct gaatgatctc ttccagacgg ccttgttcag cggccagctt gtcaaaatcg
    14701 gcatccggat cggcgtacag cgcatacact tcatccaggc gttt
//
LOCUS       DAJVYM010000098        14404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVYM010000098 DAJVYM010000000
VERSION     DAJVYM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.099"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQS60_002746"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQS60_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS2388453.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQS60_002747"
     CDS             1828..3147
                     /locus_tag="OQS60_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS2388454.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OQS60_002748"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OQS60_002748"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS2388455.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OQS60_002749"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OQS60_002749"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS2388456.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OQS60_002750"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OQS60_002750"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS2388457.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OQS60_002751"
     CDS             8179..9729
                     /locus_tag="OQS60_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2388458.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OQS60_002752"
     CDS             9731..10753
                     /locus_tag="OQS60_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2388459.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OQS60_002753"
     CDS             10750..11646
                     /locus_tag="OQS60_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS2388460.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OQS60_002754"
     CDS             11643..12629
                     /locus_tag="OQS60_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2388461.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OQS60_002755"
     CDS             12658..13548
                     /locus_tag="OQS60_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2388462.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQS60_002756"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQS60_002756"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS2388463.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3557 c         3630 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcacacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJVYM010000099        14370 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVYM010000099 DAJVYM010000000
VERSION     DAJVYM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.100"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..697)
                     /locus_tag="OQS60_002757"
     CDS             complement(<1..697)
                     /locus_tag="OQS60_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2388464.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            953..1108
                     /gene="rseD"
                     /locus_tag="OQS60_002758"
     CDS             953..1108
                     /gene="rseD"
                     /locus_tag="OQS60_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS2388465.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1105..1680
                     /gene="rpoE"
                     /locus_tag="OQS60_002759"
     CDS             1105..1680
                     /gene="rpoE"
                     /locus_tag="OQS60_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS2388466.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1713..2363
                     /gene="rseA"
                     /locus_tag="OQS60_002760"
     CDS             1713..2363
                     /gene="rseA"
                     /locus_tag="OQS60_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS2388467.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2363..3319
                     /gene="rseB"
                     /locus_tag="OQS60_002761"
     CDS             2363..3319
                     /gene="rseB"
                     /locus_tag="OQS60_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS2388468.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3316..3795
                     /gene="rseC"
                     /locus_tag="OQS60_002762"
     CDS             3316..3795
                     /gene="rseC"
                     /locus_tag="OQS60_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS2388469.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3993..5792
                     /gene="lepA"
                     /locus_tag="OQS60_002763"
     CDS             3993..5792
                     /gene="lepA"
                     /locus_tag="OQS60_002763"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS2388470.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5808..6782
                     /gene="lepB"
                     /locus_tag="OQS60_002764"
     CDS             5808..6782
                     /gene="lepB"
                     /locus_tag="OQS60_002764"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS2388471.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7055..7735
                     /gene="rnc"
                     /locus_tag="OQS60_002765"
     CDS             7055..7735
                     /gene="rnc"
                     /locus_tag="OQS60_002765"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS2388472.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7732..8637
                     /gene="era"
                     /locus_tag="OQS60_002766"
     CDS             7732..8637
                     /gene="era"
                     /locus_tag="OQS60_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS2388473.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8649..9377
                     /gene="recO"
                     /locus_tag="OQS60_002767"
     CDS             8649..9377
                     /gene="recO"
                     /locus_tag="OQS60_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS2388474.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9389..10120
                     /gene="pdxJ"
                     /locus_tag="OQS60_002768"
     CDS             9389..10120
                     /gene="pdxJ"
                     /locus_tag="OQS60_002768"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS2388475.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10120..10500
                     /locus_tag="OQS60_002769"
     CDS             10120..10500
                     /locus_tag="OQS60_002769"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS2388476.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10722..10802
                     /gene="shoB"
                     /locus_tag="OQS60_002770"
     CDS             10722..10802
                     /gene="shoB"
                     /locus_tag="OQS60_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS2388477.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10996..11256)
                     /gene="yfhL"
                     /locus_tag="OQS60_002771"
     CDS             complement(10996..11256)
                     /gene="yfhL"
                     /locus_tag="OQS60_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS2388478.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11471..12661
                     /locus_tag="OQS60_002772"
                     /pseudo
     CDS             11471..12661
                     /locus_tag="OQS60_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12846..14033)
                     /locus_tag="OQS60_002773"
     CDS             complement(12846..14033)
                     /locus_tag="OQS60_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS2388479.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14033..>14370)
                     /locus_tag="OQS60_002774"
     CDS             complement(14033..>14370)
                     /locus_tag="OQS60_002774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388480.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3622 a         3606 c         3708 g         3434 t
ORIGIN      
        1 agcccgcgcg ccacgccata gcaatgccat cgccagaaga aatatccgga ttggtggtgt
       61 actgatacac cttcgacgca ccgccggttg ccagtaccac tgcttttgcg tggcaggttt
      121 ccaccgtttc tttatttcgg ttccataccc acgcgcctac aacccgtcgc gtgcccggca
      181 ggccaatttt gtcagagaca atcagatcaa ctgcgttgct gcgctccagc acacgaatat
      241 tcggatggtt ctgcgccttg ctcaccagcg tggattgtac ttctctaccg gtggcgtcgg
      301 cggcatgaag aatacgacgg tgactatgtc caccttcacg ggtcagatgg tagctttctt
      361 cgccattcgg ttgaacgtgg gtatcaaaca aaaccccctg gtcaattaac cattgcacac
      421 aggaacgagc attgctggcg acaaattcaa ctgcatggcg atcgcaaata ccagccccgg
      481 caatcaatgt gtcttccaca tgcgagtcaa tgctgtcagt ttcatcaaac acggcggcaa
      541 taccgccctg ggcataaaat gttgaacctt ccgttaccgg gcctttactt agaacgatga
      601 cctgatgctg gtcagccagg cgtagcgcca gtgaaagtcc ggctgcgccg ctaccaataa
      661 tcaacacgtc acatgaatgt tcagggagag tattcatttt ctttgtttaa tttactaaac
      721 attgtttggt cagcatagca tcatgttgtg cgaataaaca cctgctattt taatatttgt
      781 tacagtggct aaacacgttg acgcagggcg gcgagaaaaa gagaagttac tggctggtgg
      841 aggattaggt ggtgaaataa aaaggccgtt gggttactct tcaggcagtt aaatgggcat
      901 ttctacacag ataatgcgat gttcagattc tgtagactta taatgataga taatgatccg
      961 tctacagcat gacaaacaaa aacagatgcg ttacggaact ttacaaaaac gagacactct
     1021 aaccctttgc ttgctcaaat tgcagctaat ggagtggcgt ttcgatagcg cgtggaaatt
     1081 tggtttgggg agactttacc tcggatgagc gagcagttaa cggaccaggt cctggttgaa
     1141 cgggtccaga agggagatca gaaagccttt aacttactgg tagtgcgcta tcagcataaa
     1201 gtggcgagtc tggtttcccg ctatgtgccg tcgggtgatg ttcccgatgt ggtacaagaa
     1261 gcttttatta aagcctatcg tgcgctggat tcgttccggg gagatagcgc tttttataca
     1321 tggctgtatc ggattgctgt aaatacagcg aaaaattacc tggttgctca ggggcgtcgt
     1381 ccaccttcca gtgatgtgga tgccattgaa gctgaaaact tcgaaagtgg cggcgcgttg
     1441 aaagaaattt cgaaccctga gaacttaatg ttgtcagaag aactgagaca gatagttttc
     1501 cgaactattg agtccctccc ggaagatttg cgcatggcaa taaccttgcg ggagctggat
     1561 ggcctgagct atgaagagat agccgctatc atggattgtc cggtaggtac ggtgcgttca
     1621 cgtatcttcc gagcgaggga agctattgat aacaaagttc aaccgcttat caggcgttga
     1681 cgatagcggg atactggata agggtattag gcatgcagaa agaacaactt tccgctttaa
     1741 tggatggcga aacgctggat agtgagctgc ttaacgaact ggctcataac ccagaaatgc
     1801 agaaaacctg ggaaagctat cacttaatcc gtgattcaat gcggggtgat actcccgagg
     1861 tgctccattt cgatatctct tcacgcgtga tggccgccat tgaagaagag ccagtacgtc
     1921 aaccggcgac attgatcccg gaagcccagc ctgcgccgca tcaatggcac aaaatgccat
     1981 tctggcagaa agtacgtccg tgggcggcac agcttaccca aatgggcgta gcagcatgcg
     2041 tatcgcttgc agttatcgtt ggcgtccagc actataatgg acaatctgaa acgtcccagc
     2101 agcccgaaac gccggtattt aatacactgc cgatgatggg taaagccagc ccggtaagcc
     2161 tgggagtacc ttctgaagcg accgcaaaca atggtcaaca gcagcaggta caggagcagc
     2221 gtcgtcgcat taatgcaatg ttgcaggatt acgaactgca acgccgactc cactctgaac
     2281 agcttcagtt tgagcaggcg caaacccagc aagccgctgt acaggtgcca ggacttcaaa
     2341 ctttaggaac gcaatcgcag taatgaagca actttggttt gccatgtcat tagtgacagg
     2401 tagcctgtta ttctctgcta acgcctcggc cactcccgcg tccggggcgt tattacagca
     2461 gatgaacctg gccagtcagt cactgaatta cgagctgtca ttcatcagca tcaataaaca
     2521 gggtgttgag tctctgcgtt atcgacatgc acgcctcgat aaccgtcctc ttgcacaatt
     2581 gttgcaaatg gatggcccgc gccgggaagt ggtacagcgc ggcaatgaaa tcagctattt
     2641 tgaaccggga cttgaaccgt tcacgcttaa tggcgattac attgttgatt ctctgccatc
     2701 gcttatctat accgatttca aacgcctttc tccttactac gactttatct ccgtcgggcg
     2761 cacgcgtatt gctgatcgtc tttgcgaagt cattcgcgtg gttgcccgag atggtacacg
     2821 ctacagctac atcgtgtgga tggacactga atcgaaatta ccgatgaggg ttgatctcct
     2881 tgatcgcgat ggtgaaacgc tggaacaatt tcgcgtgatt tcttttaacg tcaatcagga
     2941 tatcagcagc agtatgcaga cgctggcgaa ggcaaatttg ccgccgttgc tttctgttcc
     3001 tgtaggtgaa aaagctaaat tcagctggac gccaacctgg ttgccacagg gttttagcga
     3061 agtttccagt agtcgacgtc cgctaccgac gatggacaac atgcctatcg aatcacgtct
     3121 ctattccgac ggattattca gcttctcggt aaacgttaac cgcgctacgc catcgagcac
     3181 cgatcagatg ttgcgcaccg gacgcagaac cgtcagtaca agcgtacgtg ataacgccga
     3241 aatcaccatt gtcggtgaac tgccgccgca aacggcgaaa cgcattgccg agaatattaa
     3301 gttcggggca gcgcaatgat caaagagtgg gctaccgtcg tctcctggca aaacgggcag
     3361 gcgctggtca gttgtgatgt taaagcctca tgcagcagct gcgcttcacg cgccggttgc
     3421 ggtagccgcg tgttgaataa acttggcccg caaaccacgc ataccattgt cgtaccctgt
     3481 gatgaaccgt tagtgccggg gcaaaaagtg gaattgggga tcgccgaagg cagcctgctt
     3541 agctccgcat tactggttta tatgtcgccg ctggtgggat tattccttat cgcttcgcta
     3601 tttcagctac tctttgcttc tgacgttgca gcattatgtg gtgcgatcct tggcggtatt
     3661 ggcgggtttc tgattgcgcg cggttactcg cgtaagtttg ctgcccgggc agaatggcaa
     3721 ccgatcatat taagcgtggc attgccgcct gaccttgtgc gatttgaaac atcttcggaa
     3781 gacgcgagcc agtgatttca ctggctttgt tgcattttgc ataacaaatg cgaggacgtt
     3841 tccagatacc acaatccata cggtatgaac atttcctcgc ctcaatgttg tagtgtagaa
     3901 tgcggcgttt ctattaatac agacgttaag ctcagaacag cgacttctaa aagcctggtt
     3961 aaccgggcat taaggcacaa taatcatact ttatgaagaa tatacgtaac ttttcgatca
     4021 tagctcacat tgaccacggt aaatcgacgc tgtctgaccg tattatccag atctgcggtg
     4081 gcctgtctga ccgtgaaatg gaggcgcagg ttctcgattc catggatctt gagcgtgagc
     4141 gtggcattac catcaaagcg caaagcgtga cgctggacta caaagcgtct gacggcgaaa
     4201 cctatcagct taactttatc gacaccccgg gccacgtaga cttctcctat gaagtttccc
     4261 gttcgctggc tgcctgtgaa ggtgcattgc tggtggtcga cgccgggcag ggcgtggaag
     4321 cgcaaaccct ggcaaactgc tacaccgcca tggaaatgga tctcgaagtg gtgccagtac
     4381 tgaacaagat tgacctgccg gcagccgatc ctgaacgcgt ggcggaagaa attgaagata
     4441 tcgtcggcat cgacgccact gacgcggtgc gctgttcagc gaaaaccggc gttggcgtgc
     4501 aggacgttct cgaacgtctg gtgcgcgaca ttccgccgcc ggaaggcgat ccggaaggct
     4561 cgttgcaggc actaattatc gactcatggt tcgacaacta cctgggcgtt gtttcactta
     4621 tccgtattaa aaacggcacc ctgcgtaagg gcgacaaagt gaaagtcatg agtaccgggc
     4681 agacctataa cgccgaccgt ctgggcatct tcacgccgaa acaggttgac cgcactgaac
     4741 tgaaatgtgg cgaagtaggc tggctggtat gtgcgattaa agatatccat ggcgctccag
     4801 tcggcgatac cttaacgctg gcgcgtaatc cggcagaaaa ggcgctgcct ggctttaaga
     4861 aagtcaaacc gcaggtatac gccggtctgt tcccggtaag ttccgacgac tatgaagcct
     4921 tccgtgacgc gctgggtaaa ctcagcctga atgatgcctc actgttctat gagccggaaa
     4981 gctccagcgc gctgggcttt ggtttccgct gcggcttcct tggcctgctg cacatggaga
     5041 tcatccagga acgtctggaa cgtgaatacg atctggatct gatcaccact gcgccgaccg
     5101 tagtgtatga agttgaaacc acgtcaaggg aagttatcta cgtcgacagc ccatccaagc
     5161 tgcctgcggt aaataacatt tacgaactgc gcgagccgat tgcagagtgt cacatgctgc
     5221 tgccgcaggc atatctcggc aacgttatta cgctgtgcgt agaaaaacgc ggcgtgcaga
     5281 ccaatatggt ttaccacggt aatcaggtgg cgctgacgta cgagatcccg atggcggaag
     5341 tggtgctcga cttcttcgat cgcctgaaat ctacctcgcg tggttatgcg tctctggatt
     5401 acaacttcaa acgcttccag gcgtccgaca tggtacgtgt agacgtatta atcaacggtg
     5461 aacgtgttga tgcgttggcg ctgatcaccc accgtgataa ttcgcaaaac cgcggtcgcg
     5521 agttggtgga gaagatgaaa gatctgatcc cacgccagca gtttgatatc gccattcagg
     5581 cagcgattgg tacgcacatt attgcgcgat ccaccgtgaa acagctgcgt aaaaacgtac
     5641 tggctaaatg ttatggcggc gatatcagcc gtaagaaaaa gctgctgcag aagcagaaag
     5701 aaggtaagaa acgcatgaag cagatcggta acgtcgagct gccgcaggaa gcgttcctcg
     5761 ccattctgca cgtcggcaaa gacaacaaat aacccttagg agttggcatg gcgaatatgt
     5821 ttgccctgat tctggtgatt gccacactgg tgacgggcat tttatggtgc gtggataaat
     5881 tctttttcgc acctaaacgg cgggaacgtc aggcagcggc gcaggcggct gccggtgact
     5941 cactggataa agcaacgttg aaaaaggttg caccgaagct tggctggctg gaaaccggag
     6001 cttctgtttt tccggtgctg gctatcgtat tgattgtacg ttcgtttatt tatgaaccgt
     6061 tccagatccc gtcaggttcg atgatgccga ctctgttaat cggtgatttt attctggtag
     6121 agaagtttgc ttatggcatt aaagatccta tctaccagaa aacgctgatc gaaaccggtc
     6181 atccgaaacg cggcgatatc gtggtcttta aatatccgga agatccaaag cttgattaca
     6241 tcaagcgcgc ggtgggttta ccgggcgata aagtcactta cgatccggtc tcaaaagagc
     6301 tgacgattca accgggatgc agttccggcc aggcgtgtga aaacgcgctg ccggtcacct
     6361 actcaaacgt ggaaccgagc gatttcgttc agaccttctc acgccgtaat ggtggggaag
     6421 cgaccagcgg attctttgaa gtgccgaaaa acgaaaccaa agaaaatgga attcgtcttt
     6481 ccgagcgtaa agagacactg ggtgatgtga cgcaccgaat tctgacagtg ccgattgcgc
     6541 aggaccaggt ggggatgtat taccagcagc cagggcaaca actggcaacc tggattgttc
     6601 cgccgggaca atacttcatg atgggcgaca accgcgacaa cagcgcggac agccgttact
     6661 ggggctttgt gcctgaagcg aatctggtcg gtcgggcaac ggctatctgg atgagtttcg
     6721 ataagcaaga aggcgaatgg ccgacaggtg tgcgcttaag tcgcattggc ggcatccatt
     6781 aatagcccat cttcgttcac gttgtcgccg ttatggcgac aacgtgaatt atttatgaga
     6841 taaatctccc gtggctaacg acatcccccg tcgttgtgta tagaatattc ccccgaagtt
     6901 taaggttggc acctccaggt tgccacggca cacgaaacag cgttggtccc catataccgg
     6961 taaactgaaa ctgcagcgaa gcagttagca gagccatgta tatcaggtct gtttcgtgtg
     7021 ctgaattgtt gacgcattta tttattggta tcgcatgaac cccatcgtaa ttaatcggct
     7081 tcaacggaag ctgggctaca cttttaatca tcaggaactg ttgcagcagg cattaactca
     7141 tcgtagtgcc agcagcaaac ataacgagcg tttagaattt ttaggcgact ctattctgag
     7201 ctacgttatc gccaatgcgc tttatcaccg tttccctcgt gtggatgaag gcgatatgag
     7261 ccggatgcgc gccacgctgg tccgtggcaa tacgctggcg gaactggcgc gcgaatttga
     7321 gttaggcgag tgcttacgtt tagggccagg tgaacttaaa agcggtggat ttcgtcgtga
     7381 gtcaattctc gccgataccg tcgaagcatt aattggtggc gtattcctcg acagtgatat
     7441 tcaaaccgtc gagaaattaa tcctcaactg gtatcaaact cgtttggacg aaattagccc
     7501 aggcgataaa caaaaagatc cgaaaacgcg cttgcaagaa tatttgcagg gtcgccatct
     7561 gccgctgccg acttatctgg tagtccaggt acgtggcgaa gcgcacgatc aggaatttac
     7621 tatccactgc caggtcagcg gcctgagtga accggtggtt ggcacaggtt caagccgtcg
     7681 taaggctgag caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg aatgagcatc
     7741 gataaaagtt actgcggatt tattgccatc gtcggacgtc cgaacgttgg caaatccaca
     7801 ttgttgaaca aactgctggg gcagaagatc tccatcacct cccgcaaagc gcaaacaact
     7861 cgtcaccgca ttgtggggat ccatactgaa ggcgcgtatc aggcgatcta cgttgatacc
     7921 cctggcctgc atatggaaga aaaacgcgct attaaccgcc tgatgaacaa agcggcgagc
     7981 agctctattg gcgacgttga gctggtgatt tttgtcgttg aaggcacccg ctggacgccg
     8041 gacgacgaaa tggtgctcaa caaactgcgc gatggcaaag cgccggtgat cctcgcggtg
     8101 aacaaagtgg ataacgtgca ggaaaaagcc gatctgctgc cgcacttaca gttcctggca
     8161 agtcagatga acttcctcga tatcgtgccg atctctgctg aaaccgggct gaatgtcgac
     8221 actatcgcag caatcgtgcg taagcatctg cctgaagcga cccatcactt cccggaagat
     8281 tacatcaccg atcgctcaca gcgttttatg gcgtctgaaa tcatccgcga aaaactgatg
     8341 cgtttcctcg gcgctgaact gccgtactcc gtgaccgtgg agatcgaacg tttcgtctct
     8401 aacgaacgcg gtggttatga catcaatggt ttgattctcg ttgagcgtga agggcagaag
     8461 aagatggtta ttggcaacaa aggggccaag atcaaaacca tcgggattga agcgcgtaaa
     8521 gacatgcagg aaatgttcga agcgcctgtc caccttgaac tgtgggtaaa agtgaaatcc
     8581 ggttgggccg acgacgaacg cgcactgcgc agtctcggtt acgttgacga tctttaagag
     8641 tcactccgat ggaaggctgg cagcgcgcat ttgtcctgca tagtcgcccg tggagcgaaa
     8701 ccagcctgat gctggacgtc ttcacggagg aatcgggtcg cgtgcgtctg gttgccaaag
     8761 gcgcacgctc taaacgctct actctgaaag gtgcattaca gcctttcacc cctctcttgc
     8821 tacgtttcgg cgggcgtggc gaagtcaaaa cgctgcgcag tgctgaagct gtctcgctgg
     8881 cgctgccatt aagcggtatc acgctttaca gcggtctgta catcaacgaa cttctctccc
     8941 gcgtactgga atacgagacg cgcttctctg aactcttttt cgattacttg cactgcattc
     9001 agtctcttgc aggtgccact ggtacgccag aacccgcgct gcgccgcttt gaactggcac
     9061 tgctcgggca tctgggctat ggcgtcaatt ttacccattg cgcgggtagc ggcgagccgg
     9121 tagatgacac catgacgtat cgttatcgcg aagaaaaagg gtttatcgca agcgtcgtta
     9181 tcgacaataa aacgttcacc ggaaggcagt taaaagcgtt aaacgcgcgg gaatttcctg
     9241 acgcagacac actacgcgcc gcgaaacgct ttacccgcat ggcgcttaag ccgtatcttg
     9301 gcggtaaacc attaaagagc agggaactgt tccggcagtt tatgcctaag cgaacggtga
     9361 aaacacatta tgaatgatga ggattgtcat ggctgaatta ctgttaggcg tcaacattga
     9421 ccatattgca acgctgcgta acgcacgcgg tactgcttac ccggatccgg tgcaggcggc
     9481 gtttattgcc gagcaggcgg gagcggacgg cattaccgtg catttacgtg aagatcgccg
     9541 ccacattact gaccgcgatg tgcgcatcct gcggcagacg ctggataccc gaatgaatct
     9601 ggagatggca gtgaccgaag agatgctggc gatcgccgtt gagacgaagc cacatttttg
     9661 ctgcctggta ccggaaaagc gtcaggaagt aacaaccgaa ggcggcctgg acgttgcagg
     9721 gcagcgtgaa aaaatgcgcg atgcctgcaa acgtctgaca gatgccggga ttcagatttc
     9781 tctctttatc gacgccgatg aagagcagat caaagctgcg gcagaggttg gcgcaccgtt
     9841 tatcgagatc cacaccggtt gctatgctga tgccaaaact gacgccgaac aggcgcaaga
     9901 gctggtgcgt atcgccaaag ccgcgacctt tgccgctagt ctcggtctga aagttaacgc
     9961 cggacacggt ctgacctatc acaacgtgaa agccattgcc gccatccctg agatgcatga
    10021 actgaatatc ggtcatgcca ttattggccg tgcagtgatg accggactga aagatgcggt
    10081 ggcagaaatg aagcgtctga tgctggaagc gcgtggctaa tggcaatatt aggtttaggc
    10141 acggatatcg tagagatcgc tcgcatagaa gcagtgatcg cccgatccgg tgagcgcctg
    10201 gcacgccgcg tattaagcga taacgaatgg gcaatctgga aaacgcacca ccagccggtg
    10261 cgttttctgg cgaagcgttt tgctgtgaaa gaagccgcag caaaagcgtt tggcaccggg
    10321 atccgcaatg gtctggcgtt taatcaattt gaagtattca atgatgagct cggcaaacca
    10381 cggctacggc tatggggtga ggcattaaaa ctggcggaaa agctcggcgt tgtaaatatg
    10441 catgtaacgc tggcagatga acgccactat gcttgtgcca cggtaattat tgaaagttaa
    10501 aataataccg ttctctttaa aaagtaaaac ccccgaactg gtaaggaacg ggggttttac
    10561 tttttaactt taattttgtg cagcatgcac cctcaacaac agcgattatg gatcagtgta
    10621 cttatctttt acaagcaact tgcaatcttt agcataaaaa ctcgagcctt tacgaagaaa
    10681 gcaatactga acggctgcat tccttgttta aggaaaaacg aatgactgat tgccgatacc
    10741 tgattaaacg ggtcatcaaa atcatcattg ctgttttaca gctgatcctt ctgttcttat
    10801 aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa tcggacgcgc ctttaataac
    10861 tataaataag tgtcttggca gatactatat aaattaactt agtgaatgat tatgctaatg
    10921 tcatcaatta aataaatata atggcgttaa ggcttcccag taatataatt aatactctac
    10981 ttccagagta gaatattaaa ttttatccgc gtggtgcatc agcacaaatt tatcccacaa
    11041 ctgttcttct gtctcgacat gcgccggatc tttcacaata gtattgggga tcgggcacac
    11101 cttctggcag gttggtgtct cgtagtgccc tacgcattcg gtacacttat cgctgttaat
    11161 ctcgtagata tgatctccca ttgaaatcgc ctcattcggg cattcgggtt cacacatatc
    11221 acaattaata cagcgtttag taattagtaa agacatttca atggattacc gttaaatcat
    11281 tttaaaatca gtaagttgta tcgagtttgt atgctttact gtcattaact tactgtatgt
    11341 tgatccagtg tatttaacct tgataaactc agtccagcaa cacaaaaccg caacacattg
    11401 cattttgtcc cgtagaaaag acttgtatgt gtgagcttgt tttctgcgcc tacgcagata
    11461 aggattgaga atgccgcgca ctgtaacaca taatccggat agccccaata atgacgatgt
    11521 tttagccgca tctgaaaaat gggacgcctg taaacccccc tataccagcg cacacatgaa
    11581 aatctgtgtt gctgccgcca aaatcatcct cgctgcttcc ggcgtggctc gccgttccaa
    11641 atacgaaaaa gagaactatc tccgtatcga tttcagcaaa gccggtaagg ttacatttta
    11701 cgccgagttt ccaaaaaaga tgggcctcaa aggtaaaaag ctcggcgagt ggccggagct
    11761 cgctatccag ctggcgcgcg aaaaagcact aggtatggct gacggtggac tgcgggcaga
    11821 gtccgtacat gcagcgctgg aaatgtaccg ggatgacctc aaagccaaag tagcccggca
    11881 gaagctgagc ccggacagtt tcacaaccta cggggtgcgt atcgaccgga ttaaagcaac
    11941 gttcggcgag cgtgaggtgt tcagcgacgt aacatacaat cggctggtgg aagtgctgga
    12001 cgagtggatc gccactcgct cgaacaataa cgccctggag ttgtttgccg agctccgtcg
    12061 gttctggaag ttctgcgcac ctactctttg caacggccgc aatgttgccg ccagtctgcc
    12121 agatgattat gtttcctccc gcgtacagaa acctaccccc acacggcttt ttaccgatat
    12181 tgaatcaatc gcccgactct ggctcaatgt tgctgcctgc acctctgtac accagaagaa
    12241 tgctgttcgc ttcatgatca tcactggtgt tcgtccgatt aatgtccata acctgcgctg
    12301 ggactacgtt tacgaggagg ctggtgaaat tgtttatccg gaaggggtta tcggcatgcg
    12361 aggggctatg aaaacacaaa aggctttccg cctgccgata acgcctgaga tccggcggat
    12421 tatcgacgag cagaaagcct ggcgtgattc agttcctgag tgcaacaggg attatgtatt
    12481 tttgcagcca cgtgatccaa tgcagccatt ttcaaaacga tcactggata agctggtgaa
    12541 aacatacagc ccggacgggg ctgtaaaagg aataaaacat gatgggactg ttaaagggaa
    12601 agacggtgca tttaatacga tgtgccgtaa attccttaag agcaatgtta ttgccttgat
    12661 cgataggaat taaaacccca aaaagattaa aaaaacacca caaaacggat gtttcttcaa
    12721 caccactttt gctcatatga acggaaccga cgattaaact ggatggctct gattgattca
    12781 gggtatgaat ggcggttttt tgctccgttt ccctcaaaat ggacgcaact ttccctctgc
    12841 ggctctcagc cgcaccaccg catccgggcc agcggctcat gcatcaggac ctgctctgcc
    12901 agacggtagc cccgcttcag ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc
    12961 agcgggtcaa ccttcagtaa cctctgatac atccctctcc aggtgatttg catcgccgtt
    13021 tttctcactg tctccgttat gatgtacacc acttcttcca gtaaccgccg tttcgccgga
    13081 ctcaaaaaac cgtagtacct caccatacgg aaccccttat ccgccacatg ccaggagaac
    13141 ctttccatga actcatctcc actcatcaac aggtattctt cccgttttgt tcggtgactg
    13201 ttgtaacgca gaccgatttc atcctgaccg gcataatgct ccagacgact catcggcacc
    13261 ggtggctttt tcaggtaaga gccaaagtac accgccacat ggggggcatt atccatcacc
    13321 cgggatacgt tgacattcca gccacggcgg taatgcgtgt ccaggaagcg attccattcc
    13381 cgtttactgc ttccttctgc tgccagcgca tccggcatca ccaggtcagg gtatttccgt
    13441 gacagcaacc gtgttatccg gtagcgccac atgctcatca ccttacgggc gtaaaaatga
    13501 agatttttcc aggtgtggcc cgacgtcaca ccaccggcag ttgtcgataa atggatatgc
    13561 ggatgccact gctggtcacg cccccatgtg tggatcaccg tgaatatccc cggctccaca
    13621 tctgcctgat ggcagatttc cagtatcaca tccgctgcaa tgcggctcat ctctgtcagt
    13681 aaccaccggt tgtggaacac cagggaccag tactggcagg gaagtgtgaa cacaatatgc
    13741 tgccacgggc agtcggggac caggctcagc agatactgta tccactgtgc gccagccttc
    13801 accccgcagt gcgggcagga gcggctttta caccggaagc agaccttttt tgtatggcaa
    13861 cagtccggtg atgaacagca ccactgtgta taccccatca gtgtggtccc gcacgccatg
    13921 attttggtca ccgactcaat caccaccgga cgtactgccc tttccggctg cttctccagc
    13981 cagttaagcc agtggtttcc ctgctgaaag atatcggcaa aacggggaag catcagaagg
    14041 gcggggcgac tccgtccggc cagtgaaccg tgccacactc cgggcagtac ataccgccgg
    14101 cgctgatacc ggaaagaatg gtcgcaaatt cccgctccgt gcagcgggcg atttccggat
    14161 acccttcgtc atcaacacgt acaaaccaga agaccagctt tttgtttccc gcatccacaa
    14221 agaacggaat attcaggtct gcgcagcatt caacggcatc gtcaaaacta tcaaagcgca
    14281 gaacttctgc gtcttcttcg tcaaaaaaat catcttcgtg aagcttcacg acatagcggg
    14341 gaagtttgct tctttgagag gcgggtttac
//
LOCUS       DAJVYM010000100        14306 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVYM010000100 DAJVYM010000000
VERSION     DAJVYM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.101"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OQS60_002775"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OQS60_002775"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS2388481.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OQS60_002776"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OQS60_002776"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS2388482.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQS60_002777"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQS60_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCS2388483.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OQS60_002778"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OQS60_002778"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS2388484.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OQS60_002779"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OQS60_002779"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS2388485.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQS60_002780"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQS60_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS2388486.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQS60_002781"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQS60_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS2388487.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQS60_002782"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQS60_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS2388488.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OQS60_002783"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OQS60_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS2388489.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OQS60_002784"
     CDS             complement(13987..>14306)
                     /locus_tag="OQS60_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS2388490.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3443 a         3568 c         3995 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagcca tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJVYM010000101        14150 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVYM010000101 DAJVYM010000000
VERSION     DAJVYM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.102"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..228)
                     /locus_tag="OQS60_002785"
     CDS             complement(<1..228)
                     /locus_tag="OQS60_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCS2388491.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OQS60_002786"
     CDS             385..561
                     /locus_tag="OQS60_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS2388492.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OQS60_002787"
     CDS             1187..1486
                     /locus_tag="OQS60_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388493.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQS60_002788"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQS60_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS2388494.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OQS60_002789"
     CDS             complement(2472..2939)
                     /locus_tag="OQS60_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS2388495.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OQS60_002790"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OQS60_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS2388496.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OQS60_002791"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OQS60_002791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS2388497.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OQS60_002792"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OQS60_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS2388498.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQS60_002793"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQS60_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS2388499.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OQS60_002794"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OQS60_002794"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS2388500.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OQS60_002795"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OQS60_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS2388501.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OQS60_002796"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OQS60_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS2388502.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQS60_002797"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQS60_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS2388503.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OQS60_002798"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OQS60_002798"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS2388504.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="OQS60_002799"
     CDS             complement(13837..14082)
                     /locus_tag="OQS60_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS2388505.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3423 c         3686 g         3595 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg acgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca
//
LOCUS       DAJVYM010000102        14097 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVYM010000102 DAJVYM010000000
VERSION     DAJVYM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.103"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(3..1361)
                     /gene="hprS"
                     /locus_tag="OQS60_002800"
     CDS             complement(3..1361)
                     /gene="hprS"
                     /locus_tag="OQS60_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS2388506.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1361..2032)
                     /gene="hprR"
                     /locus_tag="OQS60_002801"
     CDS             complement(1361..2032)
                     /gene="hprR"
                     /locus_tag="OQS60_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS2388507.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2165..2578
                     /gene="hiuH"
                     /locus_tag="OQS60_002802"
     CDS             2165..2578
                     /gene="hiuH"
                     /locus_tag="OQS60_002802"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS2388508.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2687..3691
                     /gene="msrP"
                     /locus_tag="OQS60_002803"
     CDS             2687..3691
                     /gene="msrP"
                     /locus_tag="OQS60_002803"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS2388509.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3692..4327
                     /gene="msrQ"
                     /locus_tag="OQS60_002804"
     CDS             3692..4327
                     /gene="msrQ"
                     /locus_tag="OQS60_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS2388510.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4584..5234
                     /gene="zinT"
                     /locus_tag="OQS60_002805"
     CDS             4584..5234
                     /gene="zinT"
                     /locus_tag="OQS60_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS2388511.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5577..6107
                     /locus_tag="OQS60_002806"
                     /pseudo
     CDS             5577..6107
                     /locus_tag="OQS60_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6677..6766)
                     /locus_tag="OQS60_002807"
     tRNA            complement(6677..6766)
                     /locus_tag="OQS60_002807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6730..6732),aa:Ser,seq:cga)
     gene            6860..7657
                     /gene="mtfA"
                     /locus_tag="OQS60_002808"
     CDS             6860..7657
                     /gene="mtfA"
                     /locus_tag="OQS60_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS2388512.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7758..7833
                     /locus_tag="OQS60_002809"
     tRNA            7758..7833
                     /locus_tag="OQS60_002809"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7791..7793,aa:Asn,seq:gtt)
     gene            8147..14097
                     /gene="yeeJ"
                     /locus_tag="OQS60_002810"
                     /pseudo
     CDS             8147..14097
                     /gene="yeeJ"
                     /locus_tag="OQS60_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3988 a         3350 c         3168 g         3591 t
ORIGIN      
        1 tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag atagtgatac
       61 gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc aagtccctga
      121 cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt ttcaggctca
      181 tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt atcaagaaaa
      241 ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac aataagattc
      301 gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca ctcgacctta
      361 aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt ttcgacttcc
      421 ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc ccgggcaaga
      481 aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt accggcaatt
      541 gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt ctgacccagt
      601 aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg acttagacgc
      661 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      721 tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt cagcgcttcc
      781 gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag cggacttaat
      841 actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt atactgttca
      901 agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      961 tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct tctgtaaatg
     1021 ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
     1081 gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc ctgactaaca
     1141 tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc aattaacaac
     1201 tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
     1261 gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag cagcaatata
     1321 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
     1381 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1441 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1501 cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg gaggccagta
     1561 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc ctgctcacac
     1621 tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc aaagcgtgat
     1681 gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa ggttttacca
     1741 gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa tcccttgcag
     1801 taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc tgccagccat
     1861 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1921 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1981 gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc atgaaaataa
     2041 tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg taatgctgct
     2101 gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg acaaggatat
     2161 aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt cattaccttc
     2221 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaaccgg
     2281 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg gctggttaca
     2341 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     2401 tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2461 tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag tgaacgagca
     2521 ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg gcagttaatg
     2581 cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct gacagattaa
     2641 cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga aaaagaatca
     2701 atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc gtcgacaggt
     2761 gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg cgcatgccga
     2821 tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa aaccgctgga
     2881 gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag ataaagtctc
     2941 cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg ctaatgctgg
     3001 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     3061 cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     3121 ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc acaaattgct
     3181 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa tttatgcacc
     3241 ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     3301 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg tgggtgttta
     3361 tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc cgtggaaata
     3421 tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc gtccgccaac
     3481 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3541 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcatcctcga
     3601 tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3661 tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac aggtgacatg
     3721 gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3781 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3841 tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct acgcaaaaca
     3901 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3961 gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt tattaggcaa
     4021 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     4081 tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact ggcaacagtt
     4141 gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     4201 gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt tagccttacg
     4261 gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca acaaattatc
     4321 tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg gcgcgagcgc
     4381 cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa cctcaaatta
     4441 tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa actatgctct
     4501 catctcctaa gagttgttgc attttgccat atgttacaat ataacattac acatcatata
     4561 caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct gttgctttag
     4621 gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac ggcaaaccct
     4681 taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat gtacaaaacc
     4741 gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg caaagtggca
     4801 aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca tttgctgaaa
     4861 ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc attgaggacg
     4921 gcattgttga attccataga aataatgaaa caacatcctg taaatatgat tacgatggat
     4981 acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag
     5041 atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa
     5101 aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta ttaaatgaaa
     5161 tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg gtcgaggaaa
     5221 tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta agtcatcata
     5281 actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga cacgctcatc
     5341 actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa taacggattc
     5401 catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct tatattggct
     5461 ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac atctgtataa
     5521 ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct acaaaaatga
     5581 accgattctc aaaaactcaa atttatttac attggataac gctgcttttc gttgcaataa
     5641 cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact tatctgctga
     5701 tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt tcacgcctga
     5761 ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc tggcaaatga
     5821 aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt cctctgctgg
     5881 ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc aatgtgtctc
     5941 cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac gaaacctagg
     6001 caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt catcactaca
     6061 ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc gatcccctga
     6121 caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag tgcacaatgc
     6181 atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa tggtaagact
     6241 tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag tgtgatacaa
     6301 aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact ttgtcccgcg
     6361 gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt ctctacgcaa
     6421 cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta aaagaataat
     6481 ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc ttcctgtcag
     6541 atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt gacgatctaa
     6601 cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga agtcaggaga
     6661 taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga gttgccccta
     6721 ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc agatggttgc
     6781 catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt gttcaagtga
     6841 cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat cagcacatca
     6901 aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc tgacggaaca
     6961 ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc ggcttgttcc
     7021 tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc tattttgcct
     7081 acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct taatttatcc
     7141 tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc ataaccaacg
     7201 tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact ggttggatat
     7261 acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg ctcataagct
     7321 ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc gtgaggttgc
     7381 tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa tcgaattggt
     7441 tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg aatgttttgc
     7501 cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt tcccttcatt
     7561 gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc atcacgctaa
     7621 tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc agattaatta
     7681 accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct aagtaatatg
     7741 tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     7801 atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct tttttagcgc
     7861 aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt taacacctta
     7921 agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac cattttcccc
     7981 tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca cccaaaactg
     8041 cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat tttttcgctg
     8101 tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg ctacgaagaa
     8161 gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa ttaaactacg
     8221 ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg ctgcggcagc
     8281 acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg ccattgcaaa
     8341 tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg ttgccgaacg
     8401 tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt ttgctcgagg
     8461 ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta gtgaaaaaaa
     8521 attaaccccg ccgccgggta ataacagtga caacctcgag caacagatag ccagtacttc
     8581 acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg caaatatggc
     8641 gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa gccgcttcgg
     8701 taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact cccagttcga
     8761 ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc atactctcca
     8821 tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt tcactcccac
     8881 atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc actcccgcgt
     8941 cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg gctatttgcg
     9001 actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac gcccggccaa
     9061 tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg gcggtaaact
     9121 ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg atcggcaaag
     9181 taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga tgaccttcag
     9241 cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg tcgattttac
     9301 ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg ctgcacggcg
     9361 tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg ttctggaata
     9421 tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga agtcaggaga
     9481 agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct ataacgtcga
     9541 agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag atattctagt
     9601 taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct ggccgattga
     9661 agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca tggtggtcgt
     9721 tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc aaacattgaa
     9781 cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag caggtaatcc
     9841 tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca ccctttctga
     9901 ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag cgatgtctgg
     9961 cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc ccgccgtggt
    10021 gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg ataaggaccg
    10081 ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa atgacaaacc
    10141 tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg tgaaaccagg
    10201 agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct ataccgccta
    10261 taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat accgctggat
    10321 ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc agcaataatg
    10381 gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct gatgaaggaa
    10441 gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg gcaacttcct
    10501 tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt gatctgaaaa
    10561 gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg aaacaaacgt
    10621 taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag aagtcgaaaa
    10681 atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc cgggatgcaa
    10741 aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca gaggcgaaac
    10801 tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg accagtttga
    10861 aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct aatcaacagg
    10921 tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct tcaggtgata
    10981 tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat aaaaatggca
    11041 acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca agtaagttct
    11101 cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc gcctccctga
    11161 ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc aatgtcagcg
    11221 atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt ttgcaaacat
    11281 cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca acagtgaaag
    11341 atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca caggacgttg
    11401 cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat ggggaagcgc
    11461 atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg ctgggtaata
    11521 acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg gctcaggttg
    11581 tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca acgctaactg
    11641 caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca ttcagctcag
    11701 cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct ggcatcgcgc
    11761 aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca ctggctaata
    11821 atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg gcaaaaatta
    11881 tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac agctccggca
    11941 gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt gtgactgtga
    12001 acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc gtaacgaacg
    12061 aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa tcaggagcga
    12121 gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc acatcaatta
    12181 atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt tttgatacag
    12241 tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac ggcaacggtg
    12301 tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc cccagtaata
    12361 acgctatata tactaccaac cacgacggca atttttacgc aagctttacc gctacaaaag
    12421 ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga
    12481 cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag gatccggtga
    12541 ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca gagggcaatg
    12601 cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg aacttcacgc
    12661 tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc acgctgaaag
    12721 gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag agtgagcagt
    12781 tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac gttaccgagg
    12841 acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg actgatggaa
    12901 acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat gtgagcgcaa
    12961 gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag gctgaagtta
    13021 cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac aattatggtg
    13081 tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca ctagcctcct
    13141 taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg actgcaagcg
    13201 tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc ggaacctccg
    13261 tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa attcttgtga
    13321 caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa cctactgaag
    13381 tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt accagtctgg
    13441 agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct cacgtcaacg
    13501 accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag ccaccagagc
    13561 acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc gaggtctcca
    13621 tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat ggagcctcac
    13681 ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc agcccgctta
    13741 tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct gcaaatggca
    13801 ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg acgttaagtg
    13861 gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc agcaataaag
    13921 tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag acacagacaa
    13981 ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc gctgatccat
    14041 cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt gaggatg
//
LOCUS       DAJVYM010000103        14080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVYM010000103 DAJVYM010000000
VERSION     DAJVYM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.104"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OQS60_002811"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OQS60_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS2388513.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OQS60_002812"
     CDS             complement(1608..1931)
                     /locus_tag="OQS60_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS2388514.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQS60_002813"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQS60_002813"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS2388515.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQS60_002814"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQS60_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS2388516.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQS60_002815"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQS60_002815"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS2388517.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQS60_002816"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQS60_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS2388518.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OQS60_002817"
     CDS             7531..8568
                     /locus_tag="OQS60_002817"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS2388519.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQS60_002818"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQS60_002818"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS2388520.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OQS60_002819"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OQS60_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS2388521.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQS60_002820"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQS60_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS2388522.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OQS60_002821"
     CDS             10739..11620
                     /locus_tag="OQS60_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388523.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQS60_002822"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQS60_002822"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS2388524.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OQS60_002823"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OQS60_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS2388525.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
BASE COUNT         3396 a         3542 c         3689 g         3453 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcaggga ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc
//
LOCUS       DAJVYM010000104        14047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVYM010000104 DAJVYM010000000
VERSION     DAJVYM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.105"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..174)
                     /gene="yeaR"
                     /locus_tag="OQS60_002824"
     CDS             complement(<1..174)
                     /gene="yeaR"
                     /locus_tag="OQS60_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCS2388526.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADE"
     gene            complement(347..985)
                     /gene="leuE"
                     /locus_tag="OQS60_002825"
     CDS             complement(347..985)
                     /gene="leuE"
                     /locus_tag="OQS60_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCS2388527.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1112..2035)
                     /gene="dmlR"
                     /locus_tag="OQS60_002826"
     CDS             complement(1112..2035)
                     /gene="dmlR"
                     /locus_tag="OQS60_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS2388528.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            2138..3223
                     /gene="dmlA"
                     /locus_tag="OQS60_002827"
     CDS             2138..3223
                     /gene="dmlA"
                     /locus_tag="OQS60_002827"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS2388529.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            3474..5084
                     /gene="yeaV"
                     /locus_tag="OQS60_002828"
     CDS             3474..5084
                     /gene="yeaV"
                     /locus_tag="OQS60_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS2388530.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5116..6240
                     /gene="yeaW"
                     /locus_tag="OQS60_002829"
     CDS             5116..6240
                     /gene="yeaW"
                     /locus_tag="OQS60_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS2388531.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6296..7261
                     /gene="yeaX"
                     /locus_tag="OQS60_002830"
     CDS             6296..7261
                     /gene="yeaX"
                     /locus_tag="OQS60_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS2388532.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     HHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7315..8430)
                     /gene="rnd"
                     /locus_tag="OQS60_002831"
     CDS             complement(7315..8430)
                     /gene="rnd"
                     /locus_tag="OQS60_002831"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS2388533.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8512..10197)
                     /gene="fadD"
                     /locus_tag="OQS60_002832"
     CDS             complement(8512..10197)
                     /gene="fadD"
                     /locus_tag="OQS60_002832"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS2388534.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10402..10983)
                     /gene="yeaY"
                     /locus_tag="OQS60_002833"
     CDS             complement(10402..10983)
                     /gene="yeaY"
                     /locus_tag="OQS60_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS2388535.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(11023..11718)
                     /gene="tsaB"
                     /locus_tag="OQS60_002834"
     CDS             complement(11023..11718)
                     /gene="tsaB"
                     /locus_tag="OQS60_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS2388536.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(11776..13686)
                     /locus_tag="OQS60_002835"
     CDS             complement(11776..13686)
                     /locus_tag="OQS60_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS2388537.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13818..>14047
                     /locus_tag="OQS60_002836"
     CDS             13818..>14047
                     /locus_tag="OQS60_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS2388538.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAM"
BASE COUNT         3405 a         3724 c         3471 g         3447 t
ORIGIN      
        1 ttcatcagcg tagccgagat atttgaccgc cccatgcata acggaaaggc gtgggtaaac
       61 ccccgggcgc gttcctttat caagatgacg ttcgaatatt ccggcaggcg cagtttgttt
      121 attccagaaa ggcgttgagc gcgtatgaat ataattctgt gggatttgca gcatcctttt
      181 ccctccttcg gtgaatgcgc tgaaaacggt ttattccagc cgtttcaggg tacgcctgat
      241 aatttgcatt ttaaatacca tttattggtt actttttagc accatatcag agaaaaatca
      301 gggaggatta caagtgggaa gcccgcgaca acgcgggctg aatgtatcag gattgcagcg
      361 tcgccagtcg ggcggcgaaa cccacgaaca tcaaaccaat cagtgagttg ccaactttag
      421 ccagtttctt tttggtacgt atgtactgcg tgacaaaagc accagaaata atcaggaagc
      481 tcaaatagca gaaactcacc agttccagcg tcgtcgccag aataaagaat gaaattcccg
      541 tatgtggggc attaacatcg ataaactgta cgaaaaacga cacatagaac aaaatggctt
      601 tcggattagt caggctcaaa attaacgcgc gtttaaaaat ggccccgtat tggggctcat
      661 cggatttggc ctcgttattt ttacccttca gggtcgcgta gagaatttta ctccccagat
      721 agagcaaata aaacgcacca agataacgta caatgttgaa taatatcggg gtggtcttaa
      781 ttaatgtcgc cactccagcc catgccagaa acatcaatac tgcatcgcca ataaatacac
      841 cgcaggccgc aagataaccg cctttcatac cgctactgac gctatttttg agtacaaaca
      901 gggtatttgg ccctggcacc aacacaataa aaatggcccc aaccagatag gtccagtaat
      961 tcagaacccc gtattcagcg aacacattaa cctctttaat tatctttcga tcatgcgcga
     1021 ttaaaggtga atatgctaac caatctgtag cggcttagaa aggagaaaat caggttttaa
     1081 cccgatatca acccgataat tgaataatta atcaggcatg ctccagtgaa aaattcgggt
     1141 agtgctcggc aaaatactgg cgtaaaaatt ctatcgttat ccgcactttc gctgacgtcg
     1201 ccagccttga aacataaacg gcccagacgt tcgctggctg gtaatattcc ggtagcactt
     1261 gcactaaatg accactggca atgttttcgc taacatccca ccaggagcgc agagcaatcc
     1321 cctgcccatc gagacaccac tgatggacaa tttctccatg attagacgac agtgggccgg
     1381 tgactttaat cgcatggtgg ccttctttat tgcgtaattg ccagacacca aaaggatgat
     1441 cgcgctcttt aatgaccagg cacggcaatg cggaaagatc ggttaaatgt ttcggcgcgc
     1501 catgttgcgc aataaattcc ggcgacgcgc agagaatgcg gtaattagtc gccagtttgc
     1561 gggcgattaa attaggggcg atgtcatcgc caatgcgaat atcgagatca acaccttcat
     1621 tgaccaaatc gaccaaccgg tcttccacat caaaacgtaa ttccagttgc ggatacgcct
     1681 tagccagcgc tgataacgcc ggagccacca cctgtcgtcc aaaaccaaag ctgctgataa
     1741 tacgcagcat cccctgcggc acctgacgca cgtcagaaag ttcgtccatc atctgaccga
     1801 catcctgcaa aatccgctgc gcccattcat aaatccgctc tccttcttcg gtaatggtga
     1861 cgcggcgggt ggtgcggtgt aacaacacca cgtttagcgt ttgctccagc aaggcgatgc
     1921 gcttgctgac gaacgccggt gaaacgccca gttcttccgc cacggcggca aaaccagccc
     1981 ggcgagccac cagcataaag acgcgcaaat cgttcagcag cggtaaatta ttcatgattc
     2041 gtgttttatg tttcaccagt tacggggatt aattcctttt cagtcaatta taggatggtg
     2101 atgttgtcaa ttttgatggt caggaagtga gaacccaatg atgaaaacga tgcgtattgc
     2161 tgcgatcccg ggagatggga ttggcaaaga agtccttcct gaagggattc gcgtattaca
     2221 ggctgccgct gagcgctggg gcttcgactt gagttttgag caaatggagt gggcgagctg
     2281 cgagtattac agccatcacg gtaaaatgat gccggacgac tggcatgagc aacttagccg
     2341 tttcgacgcc atctattttg gtgccgtcgg ctggccggat accgttccgg accatatttc
     2401 gttgtggggt tcgctgctga aatttcgtcg tgaattcgac cagtacgtca acctgcgccc
     2461 ggttcgtctc tttcctggcg ttccctgccc gctggcggga aaacagcctg gcgacatcga
     2521 tttttacgtg gtcagggaaa acaccgaagg cgaatattcc tcgctcggcg gtagagtgaa
     2581 tgaaggtaca gagcatgaag tcgtcattca ggaatcggta tttacgcgtc gtggtgtcga
     2641 tcgcattttg cgttatgcct tcgaacttgc gcaaagccgc ccacgtaaga cgctaacttc
     2701 tgccactaaa tcaaacggtt tagccatcag catgccgtac tgggatgagc gagtggaagc
     2761 aatggccgag aattacccgg agatccgctg ggacaagcag catattgata ttctctgcgc
     2821 gcgttttgtg atgcagccgg aacgcttcga tgtggtggtg gcgtccaatt tgtttggcga
     2881 tatcctttcc gatcttggcc cggcctgcac cggcaccatt ggcattgccc catccgccaa
     2941 cctgaatccg gaacgcactt tcccgtcgct cttcgagcct gtccacggtt ccgcgccgga
     3001 tatctacggg aaaaatattg ctaaccctat cgccacaatt tgggccggtg cgatgatgct
     3061 cgattttctc ggcaatggcg atgagcgttt ccagcaagcg cataacggta ttctggcagc
     3121 gattgaagaa gtgattgctc acgggccgaa aacaccggat atgaaaggca gtgccaccac
     3181 gccacaggtt gccgacgcga tttgcaaaat tattttgcgt taaggtcaaa ccagtttatt
     3241 tgaaccgcgt cactgacgcg gtttttttat tcgttctttg cagttaataa cctgcgtcat
     3301 ttcacctttt attgtttccg tttcgtgttt tatggctttc cgtattctta attgtttaat
     3361 ttatgtaaca tgcaaataat caatttgtta aacacaacat acaactcatt accctgcaaa
     3421 taattacaac tggcaaaagc cagagacggg cttttatgcc caggagatga ctcatgatga
     3481 gcaatgttaa gaaaaaagat gtgccgctga taagcatcag cctggtggcc attcttttca
     3541 tcgcagctgc attaagcctt ttcccacaac aatcggccga cgcggccaac acaatataca
     3601 cttttgttac gcgtacgtta ggttccgccg tacaggtatt ggttttgctg gcaatgggac
     3661 tggtgattta tttagccacc agtaaatacg gcaatattcg tcttggcgaa ggaaaaccgg
     3721 aatacagcac gctctcctgg ctgtttatgt ttatttgtgc cggtttaggt tcttctacgc
     3781 tttattgggg ggttgctgaa tgggcctatt attatcaaac gcctggatta aatatcgcac
     3841 cacgttcaca acaggcactc gaatttagcg tcccttactc cttcttccac tggggcatca
     3901 gcacctgggc aacttatacg ctggcctcat taatcatggc ttatcacttt catgtgcgta
     3961 aaaacaaagg tctgagtctt tccggcatta ttgccgcgat taccggcgtt cgcccgcaag
     4021 gcccatgggg aaaactggtt gatttgatgt ttctgatcgc cactgtcggc gcactgacta
     4081 tttcccttgt tgtcaccgca gcaaccttta ctcgtggact ttccgcgctg accggtttac
     4141 ccgataactt caccgtgcag gcatttgtga tcctgctttc cggcggcatt ttttgcctaa
     4201 gctcgtggat tggtatcaac aacggtttgc aacgtctgag caaaatggtt ggctggggcg
     4261 cgttcctgct gccattactg gtgctgattg tcggtccaac cgaatttatt accaacagca
     4321 tcatcaatgc catcggcctg accacgcaaa acttcctgca aatgagctta ttcaccgatc
     4381 cgcttggcga tggttcattt acccgcaact ggaccgtttt ctactggctg tggtggatct
     4441 catacccccc tggcgtagca atgtttgtca cccgcgtttc ccgcggtcgt aagattaaag
     4501 aagttatctg gggactgatc ctcggcagca ccgtcggttg ctggttcttc ttcggcgtaa
     4561 tggaaagcta tgccattcat cagtttatca atggcgtaat caacgtccca caggtgctgg
     4621 aaacactggg cggcgaaaca gctgtacagc aagttctgat gtcgttgcca gccggtaaat
     4681 tgttcctcgc cgcatacctg ggcgtgatga ttattttcct tgcctcgcat atggatgcag
     4741 tggcctacac catggcggcg accagtacgc gtaatctcca ggaaggtgac gatcctgacc
     4801 gtgggctgcg tcttttctgg tgcgtggtga tcactctgat cccgctttcc atcttgttta
     4861 ccggtgcttc gctggaaacg atgaaaacca ccgtcgtgct cacagccctt cccttcctcg
     4921 tcattttact ggtgaaagtc ggcggattta ttcgctggct gaaacaggat tatgccgaca
     4981 ttccggctca tcaagttgaa cattatctcc cgcagacacc ggttgaagcc ctggaaaaaa
     5041 caccagtgct ccctgcggga accgtattca aaggcgacaa ctgagcgcca tcatcctaac
     5101 gataaaggta tccctatgag caatctgagc cctgactttg tactacccga aaatttttgc
     5161 gctaacccgc aagaggcgtg gaccattcct gcccgttttt ataccgatca gaacgcgttt
     5221 gaacacgaaa aagagaacgt cttcgccaaa agctggattt gcgtcgctca cagcagcgaa
     5281 ctggcgaatg ccaatgatta tgtgacgcgt gagatcattg gcgaaagcat cgtgctggta
     5341 cgcggtcgtg ataaggtttt gcgcgcgttc tataacgtgt gtccgcaccg tggtcatcag
     5401 ttgttgagcg gtgaaggaaa agcaaaaaat gtgattacct gcccatatca cgcatgggca
     5461 ttcaaactcg atggcaatct ggcccatgca cgtaactgcg aaaacgtcgc caatttcgat
     5521 agcgacaaag cgcaactggt tccggtgcgt ctggaggaat atgccggatt cgtcttcatc
     5581 aacatggacc ccaacgccac cagcgtagaa gatcagttac ccggtctggg cgcgaaagtg
     5641 ctggaagcct gcccggaagt ccacgatctg aaactggcgg cccgctttac cacccgcacg
     5701 cctgccaact ggaagaacat tgtcgataac tatctcgagt gctatcactg tggtccggcg
     5761 catccaggtt tctccgactc cgtacaggtt gatcgctact ggcacaccat gcacggtaac
     5821 tggacgctgc aatacggttt cgccaaaccg tccgaacagt cgtttaaatt tgaagagggt
     5881 acggatgcgg cattccacgg tttctggctg tggccgtgca cgatgctgaa cgtcaccccg
     5941 atcaaaggga tgatgacggt catttatgaa ttcccggtgg attctgaaac taccctgcaa
     6001 aactacgata tttacttcac caatgaagag ttaaccgacg agcaaaaatc gctgattgag
     6061 tggtatcgcg atgtgttccg tccggaagat ttacgtctgg ttgaaagcgt acagaaaggg
     6121 ctgaaatcgc gtggctatcg tggtcagggg cgcatcatgg ccgacagtag cggtagtggc
     6181 atttccgaac atggtatcgc ccatttccat aatctgctgg cgcaggtgtt taaggactaa
     6241 tgacatcggc ggcggtattt tccgccgctg ggctgatttt tgatggagta cagcaatgtc
     6301 agactatcaa atgtttgaag tacaggtgag tcaggtggaa ccgcttaccg aacaggtgaa
     6361 acgcttcacg ctggtggcaa ccgatggcaa accgctgcct gcgttcaccg gaggaagtca
     6421 catcattgtg cagatgagcg atggtgataa ccagtacagc aatgcgtatt cactactgag
     6481 ttcgccgcat gacacctctt gttatcagat tgccgttcgg ctggaggaaa actcgcgcgg
     6541 cggttcccgc tttttgcatc agcaggtaaa agtgggcgat cggttaacga tttcaacgcc
     6601 taataacctg tttgcgctaa ttccctcagc cagaaagcat ctgtttatcg cgggcggtat
     6661 tggtatcacc cctttcctgt cgcacatggc agagttgcaa cacagcgacg tcgactggca
     6721 gctacattac tgctcgcgaa atccagaaag ttgcgcattt cgtgatgagc tagtccatca
     6781 tccgcaggct gagaaagtcc atttgcatca ttcgtcaacc ggaacacgac tggaattagc
     6841 gcgattattg gcggatatcg aacctggcac acacgtttat acctgtggcc ccgaggcgct
     6901 aaatgaagcg gtaagaagtg aagctgcgcg tctggacatc gccgccgata cgctgcactt
     6961 tgagcaattt gctatcgaag acaaaaccgg cgatgccttt accctggtgc ttgcccgttc
     7021 cggaaaagag tttgtggtgc cggaagagat gactattttg caggttattg aaaataataa
     7081 agccgcgaaa gtggaatgtt tatgtcgtga aggggtatgc ggaacctgcg aaacagcaat
     7141 actggaaggt gaagctgacc atcgggatca gtattttagc gatgaagagc gtgccagcca
     7201 gcaaagtatg ttgatctgtt gttcgcgtgc gaagggtaaa cgcctggtgt tggatttgta
     7261 gtttgccaaa gccggatgtg gcgctgagcg cgcccagtcc ggcttcggaa gattttactg
     7321 cggatattcc tgcaataaat tgtgtaatgc ttccgccatc agctcaccac gccagccgga
     7381 aatcagctcc ggcaaattgt tctgcggttt cagtttccag tgccagttca gcagttggtt
     7441 gatttgccga cgcgatgcca gcaattcggc gctgatctta tgcgtttcgc tcacgtcagt
     7501 aatcagcgac ttaatcgctt taaacgcttt acgataaccc ggcatgtcca tcaggttaag
     7561 catcggctgc ggtaaggcct cttccggcaa tgtctgcgct ttttccacca gcgccagcag
     7621 cgttttaccg tgaaagcgga tttcgctacc ggataaaccc aggctgtcca gttcgcctaa
     7681 acttcccggc atataacgcg cgaccgacca caaatgctct tcacgcacga caaagttcac
     7741 cgccagatca cgctcacgag ccttgcgcag tcgccagtcg gctaacagtt gcagacaggc
     7801 cagttggcgt gtgcgtaatt gccaggcatt ggtgatatca cgccaggcat cttccggcgc
     7861 aacgacttcc tgacgacgca tttgcatcag gcggcattca tccagcgccg caggtagcca
     7921 gccggaggcc tccgtttcta ccataagctt ggcggtgatc ggtaacagat accagacatc
     7981 cgccgctgcg tattcacact gacgttcggt cagcggtctg gccagccagt cggtgcgcga
     8041 ttcactcttg tccagcgtaa cgccggaata ctcttccacc atggaagcga aaccccatga
     8101 catcgggcgt ccgcagaagg cagcaaggat ttgcgtgtca atcaggggtt gtggtaattc
     8161 gccaaagaca ttgaggaaca cttccagatc ttcactgcct gcatggagaa attttgtgat
     8221 ggacggatcg cgcaggatcg ctttcagcgg tgaccagtcg gtgatcccga gaggatcaat
     8281 tagcgccaga tgctcaccat cgaaaagttg aatcaacccc agctgcgggt aataagtgcg
     8341 cgtacgaaca aattcagtat cctgggctat cgccggaaag acacggacgg cttcacacaa
     8401 agaagccagc gcatcgtccg tggtaatcat ttggtaattc aaattgtatt ctctttagtg
     8461 ggcgtcaaaa aaaacgccgg attaaccggc gtctgacgac tgacttaacg ctcaggcttt
     8521 attgtccact ttgccgcgcg cttcgtcacg taattctcgt cgcaaaattt ttccgacgtt
     8581 agatttcggt aactcatcac gaaactccac cagcttcggt actttgtagc ccgtgagctg
     8641 acggcagcaa aaggtcacca gtgactcttc ggtaagcgat ggatcttttt tcactacgaa
     8701 gattttcacc gcttcaccac tggagccgga aggtacgcca acagccgcga cttcctgtac
     8761 gccaggatgc tgcatgacga catcttcaat ctcgttggga tagacgttaa aaccggaaac
     8821 cagaatcatg tcttttttac gatcgacaat gcgcaggaac ccttcttcat ccatcaccgc
     8881 gatgtcgccg gtgtgtaacc agccattttt gatgatctca tcggtagcat cgggacgctg
     8941 ccagtaaccc agcatcacct gcggtccttt gacacaaagc tcacccggtt gacccggtgg
     9001 tacttcatta tcgtcgtcat ccaccagttt ggcttccgtc gacggcaccg gcaaaccgat
     9061 gctaccacta tgataatcaa tatcatatgg gttaacgctg accagcggcg cacactcggt
     9121 aagaccatag ccttccagca gatactgtcc tgtcagtttc acccaacgct ctgccaccac
     9181 ttgctgcact ggcatcccac cgcctgcgga aagatgcaga ctggagaaat ccagctgctg
     9241 gaactcttta ttgttcagca acgcattgaa caaggtgtta acgccagtga tagcggtaaa
     9301 cggatatttc gctaactctt ttaccaaccc tggaatatcg cgcgggttag tgataagcag
     9361 gttctgccca cccagttcga taaacagcag gcagttaatg gtcagggcaa aaatgtgata
     9421 cagcggcagt gccgttacta ccagttcttt acctggatgc agcaacggcc cataggtggc
     9481 gttgacctgt tccaggttcg ccagcatatt gcggtgagtc agcatcgcgc ctttcgccac
     9541 acctgtggtg ccgccggtgt attgcaggaa ggctaaatct tccggcacca gttcgggttt
     9601 gacgtactgc atccggtagc cgttatgcag tgcgctacga aatgaaatgg catctggcag
     9661 atggtatttc ggcaccaaac gcttgatgta tttaacaacg aaattgacta ccgtgccttt
     9721 tgccgtagat agctgatcgc ccatacgggt cagaattacg tgctgaacgg cggttttatc
     9781 aaccactttt tccagtgtgt gagcaaagtt agacacgata acaatcgccg atgcgccgct
     9841 atcgttaagc tgatgctcaa gctcacgcgg ggtatacaac gggttaacgt ttacgacgat
     9901 catcccggca cgcaaaatgc caaacagcgc caccggatat tgcaataaat taggcatcat
     9961 caacgcaacg cgatcgcctt tcttcagccc caacccttgt tgcaaataag cggcaaacgc
    10021 gcgactgcgt tcttccagct tgcggaaggt cattacctcc cccatattca caaacgcagg
    10081 ctgatcggcg tagcggacga ccgactgctc aaacatatct atcagagatt gataacggtc
    10141 agggttgatc tccgtcggaa catccgcggg ataacggtta agccaaacct tcttcaattc
    10201 ttcacctcta aaatgcgtgt tcgtcgtcat cgcaacccca agtgataaac atgttgttaa
    10261 cataacatta actcatctta ccagcttgat aattagacaa cgaaaaggtt gcgaagcgcg
    10321 tcactattta tttttatagt tgccgtatga acgcagaaac agcggaccag ccgctgtttc
    10381 tttttcttta aaaacaactt gttactcggt tacaattgtt tcaactcttg cagggccagg
    10441 attataccaa ccccatccgc cgtaaccata gggccagccg cgtccaccat aaaaccatgg
    10501 atcaattggc tgcggcggca taatcacctg ttgagtgata tgccagcgtt tgtaacctgt
    10561 tacctgcata accataaatt tatatggtgc attatttact ttgccatcca ctgtgccagt
    10621 gattggcccg acaaccgtca ccagttgtcc acgaaaatcg accggatcaa gaaaaccgtt
    10681 cacatcggca taaatgcgcc cgcgtgaagg ttctcccaag gttggtcttg ctccgctgtc
    10741 cagcggtacg gtagcaattt ccaggcgggt tttcccttgc tggttttgta ccacaaccac
    10801 tttgccgcca aagcgcgcct cctgaccaac atataaatct ggcgcattca tcacgcgtac
    10861 cagatcttgc tgtggtgttg gactgctgcc tttaatggcg tccggcacag tgacacaacc
    10921 gctcagcatt agcgcaaacg tacctgccag tatgcctttg ataacattct tttgaaccgc
    10981 catgatgcga ctcctttttt tctcagggca tactcttaag attcattctt tgcccggaag
    11041 tttcttccat gcgacgttgt tacgtaaata aaccggttcg gcatgttcca ccgccaccgt
    11101 tttaccctcg gcaaacatct gacacgcaat cggcagcata tcttccgccg ctggcagtaa
    11161 aacttctccg tcgcgcaaaa ccagcccgct ctctttacgg agatccggcc aggcttgcca
    11221 gcccgtccct accgttaccc attcgccgga aagctgttgc attcgttcat gaacgagttc
    11281 gggtttgagt acggcttcgg tttcttcacc gcgccagata ccgttttcat cacgctgata
    11341 ttcggcccag taaacttcgc ccattcgcgc gtcaatggct gccagcacgc gggttgcgcc
    11401 gtttttgcgc cacgccccct gcgccatcgt cattagcgta gagacaccaa tcatcggtaa
    11461 ctccgcgcca agcgccagcc cttgcgcaat accaatacca atgcgcacgc cagtaaagct
    11521 accggggccg cgcccgtaag ccagagcgtt tatgtcagtc agggaagttc cgctggtggt
    11581 caggatatcc tgcaccatcg gtaagattcg ttgagtatgt tcacgagggc aaagctcaaa
    11641 atgagcgttg acattaccgt cgttccacag agcgacagag cagacctctg tcgcggtatc
    11701 gatagccaga attcgcatgg gtcttcgtgc ttagatcaat aaaaaggcgc gcatcatacc
    11761 atactccgta acaaattacc tggaggatgg tatcgcaagg aaacgaaccg cacgggcaat
    11821 gtcacgggtg cgtggcgcgg gcggcagact ggcgagaaac gtcgcgccgt aaggacgcat
    11881 caccagccga ttgtcacaaa ttaccagcac gccgcgatcg tcggcgtcgc gaatcagtcg
    11941 ccctactccc tgcttgagag taatgaccgc atccggcagc tgcacttcat cgaacgggtc
    12001 gccaccgcgc aaacgacaat cttccatgcg cgcttttaac agtagatcat ccggcgaggt
    12061 aaacggcaat ttgtcgataa ttaccaatga caatgtatcg ccacgcacgt ccaccccttc
    12121 ccagaaacta ctggttgcca caagaagcgc gttaccggcg ctgacaaatt gctgcaacag
    12181 ttgccctttg ctggtttccc cctgcaacaa aacgggaagc gtcatggtag cgcggaactg
    12241 ctcggccaga tcgcgcatca tggcatgcga ggtacaaagc ataaaacaac gaccgttgtt
    12301 agcttcgatg atcggtcgca gcattgccgc cagttgccgt gcacaacccg gttgattggt
    12361 ttgcggcagg ttgcgcggca cacagagtaa cgcctggcgg ctgtaatcaa acgggcttgg
    12421 caatagcaac gactccgcct gttctatccc aagccgcgag gtgaaatgat gcagatcgtc
    12481 gttcaccgac agcgttgctg aggtgaagat ccagctaccg ggcttttgcg ccattaactc
    12541 tttgaattta tccgccacgc tgagcggcgt gagagccaga gtaaaatggc gcgaagtgca
    12601 ttcgtaccag tagctgtagc ccggctgatt gatctctttt agccgcttca gccgtgtgcg
    12661 atacaacgtg gcgcgctcaa atgccgcatc cagcaaggcg gaacgcccca gtgacagttt
    12721 cgccacgtca taacaaagtt ccagggtgtc atcgagcagt aaaaatgccc gctgaatttg
    12781 cggattagct aacagctcac gcaggttacc gcgataacct ggctcaccga gttgcagacg
    12841 aaaatcctgt gcactctggg caagacgatc agcgcacttt tgtaactgct gggtgtcttt
    12901 taattcggtg cggtaggcga tggtgatgtc ttttgccagg tcgagcagtt gtcgactgga
    12961 gagtgactga ccaaaatact ggctggcaat gtccggtagc tggtgggctt cgtcgaagat
    13021 catgacgtcc gcttcaggga tcaactcgcc aaatccactc tctttaacca ccatatccgc
    13081 cagaaagaga tgatggttta ccaccaccac atcggcgtcc atcgcttttt tacgtgcttt
    13141 gaccacaaag caatctttat acatcgggca gtcgctgcca agacagttgt cgttggtgct
    13201 ggtgaccagc ggccacgcct gtgaatcttc cgccacgctg acgcaggtgc tgatatcacc
    13261 atcgactgtt tgattagacc aggagcgcag caggatcaca tcgcttaaga tttgtaccgg
    13321 cagatcgccc cccgccagcg cctgctgttc gagacgttcg aggcagaggt agtttgagcg
    13381 ccctttcagc agcgccacgt tgcccgtata tttcaatgcc tttgagactg ttggcaaatc
    13441 gcggctgtag agctgatcct gcaacgcttt tgagccggtc gagataatga ctttcttttt
    13501 cgcccgcagc gcaggagcca ggtaagcgta ggttttgccc gtaccggttc ctgcttccac
    13561 caccagcggc tggccttttt ctatcgcctg ggtgacggct accgccatct gtcgctgtgg
    13621 ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga ccgtctggtg caaaatcgtc
    13681 cgtcacacta ccccctgttg atttgaacag ggattatgtc aggatgaggg cgctttcgcc
    13741 agttgaagtg gtgacggcaa cctcacattg tggcagtctt tgcagcacga aaatggaagt
    13801 ataatgagga caaaatgatg actatcgttc gtatcgatgc tgaagcccgc tggtctgatg
    13861 tagtaatcca caacaacacg ctctactaca ctggtgtacc ggaaaacctc gacgccgatg
    13921 cctttgagca aaccgccaac acgctggcac agattgacgc cgtgctggaa aaacagggca
    13981 gcaataaatc gagcattctg gatgccacca ttttcctggc cgataaaaac gacttcgcgg
    14041 cgatgaa
//
LOCUS       DAJVYM010000105        13964 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVYM010000105 DAJVYM010000000
VERSION     DAJVYM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.106"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OQS60_002837"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OQS60_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS2388539.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OQS60_002838"
     CDS             2273..2662
                     /locus_tag="OQS60_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS2388540.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OQS60_002839"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OQS60_002839"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS2388541.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQS60_002840"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQS60_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS2388542.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OQS60_002841"
     CDS             complement(4321..4521)
                     /locus_tag="OQS60_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS2388543.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OQS60_002842"
     CDS             4687..5232
                     /locus_tag="OQS60_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS2388544.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OQS60_002843"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OQS60_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OQS60_002844"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OQS60_002844"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS2388545.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OQS60_002845"
     CDS             complement(6529..7353)
                     /locus_tag="OQS60_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS2388546.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQS60_002846"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQS60_002846"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS2388547.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQS60_002847"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQS60_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS2388548.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQS60_002848"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQS60_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS2388549.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQS60_002849"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQS60_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS2388550.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQS60_002850"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQS60_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS2388551.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQS60_002851"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQS60_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS2388552.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OQS60_002852"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OQS60_002852"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS2388553.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3390 a         3535 c         3712 g         3327 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DAJVYM010000106        13904 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVYM010000106 DAJVYM010000000
VERSION     DAJVYM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.107"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            243..1496
                     /gene="kch"
                     /locus_tag="OQS60_002853"
     CDS             243..1496
                     /gene="kch"
                     /locus_tag="OQS60_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS2388554.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1551..1724)
                     /locus_tag="OQS60_002854"
     CDS             complement(1551..1724)
                     /locus_tag="OQS60_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS2388555.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1867..3327
                     /gene="cls"
                     /locus_tag="OQS60_002855"
     CDS             1867..3327
                     /gene="cls"
                     /locus_tag="OQS60_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS2388556.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3362..3691
                     /locus_tag="OQS60_002856"
     CDS             3362..3691
                     /locus_tag="OQS60_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS2388557.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3744..4748)
                     /gene="oppF"
                     /locus_tag="OQS60_002857"
     CDS             complement(3744..4748)
                     /gene="oppF"
                     /locus_tag="OQS60_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS2388558.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4745..5758)
                     /gene="oppD"
                     /locus_tag="OQS60_002858"
     CDS             complement(4745..5758)
                     /gene="oppD"
                     /locus_tag="OQS60_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS2388559.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5770..6678)
                     /gene="oppC"
                     /locus_tag="OQS60_002859"
     CDS             complement(5770..6678)
                     /gene="oppC"
                     /locus_tag="OQS60_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS2388560.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6693..7613)
                     /gene="oppB"
                     /locus_tag="OQS60_002860"
     CDS             complement(6693..7613)
                     /gene="oppB"
                     /locus_tag="OQS60_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS2388561.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7699..9330)
                     /gene="oppA"
                     /locus_tag="OQS60_002861"
     CDS             complement(7699..9330)
                     /gene="oppA"
                     /locus_tag="OQS60_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS2388562.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10068..10715)
                     /gene="ychE"
                     /locus_tag="OQS60_002862"
     CDS             complement(10068..10715)
                     /gene="ychE"
                     /locus_tag="OQS60_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS2388563.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11193..13868
                     /gene="adhE"
                     /locus_tag="OQS60_002863"
                     /gene_synonym="adhC"
     CDS             11193..13868
                     /gene="adhE"
                     /locus_tag="OQS60_002863"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS2388564.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3438 c         3230 g         3578 t
ORIGIN      
        1 ccttatagcc ggtcagtgca ttcactacca caagcagata ataactttat ctgtatttct
       61 ttatatgatg tgagcatatt caggaaaaat gacctttcct gaggtacagt tgctcgctca
      121 aatcctttga ttactgcgga taaagtcatc tactattgtt taacacattc acgcgctgca
      181 tgtatgttca gaaaatgtgc acgctaagat aattaaacaa aattgtggtt acagaggtat
      241 cagtgagtca ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg acccttcggc
      301 acgacattct cgcgctggcc gtctttttaa atggattgct tatttttaaa acaatctatg
      361 gtatgtcggt caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct
      421 ccttgctggc aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg
      481 gcttactgtt ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc
      541 taatttacac cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta
      601 cgctggtgtt tttgctgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga
      661 caatttttgc atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcgcttt
      721 atttaagcga aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga
      781 tagaaaccat gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa tcagcacgat
      841 tgttcactat ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa
      901 tttttggtcc gcttatccgc gggggattca acaaacttgt aaaaggaaac aatcatacaa
      961 tgcatcgtaa agatcatttt attgtttgcg gacattcgat tctcgccatc aatacgatcc
     1021 tgcaactgaa tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata
     1081 tcaagcaact tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg
     1141 acagttcagt attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg
     1201 ataacgatgc agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca
     1261 aaacagttct cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg
     1321 atatcattct ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg
     1381 aagagattaa taatgatatg ctcgtttcaa tgttgttgaa ctccggtcat ggcattttca
     1441 gcgataacga tgaacaagaa acgaaagctg acagtaaaga atcagtgcaa aaatagagtt
     1501 taaagacgaa agagtaaact tattgggcac cgcaatggtg cccagtatat ttagatattg
     1561 tagatcgcaa acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg
     1621 atacgcatat ttcggcgcga ttgcccctca agccaacgcg atttacgtcg gctagcctga
     1681 cgctgcatgc gccagcgccc cacttccgtt ctactccgct tcatgttaac tactctatgc
     1741 aataacagaa tggtcattat acccccaccc ccgacgctga ccagtgcttt tcccgcgttt
     1801 ttatttgcca gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca aaaggatttc
     1861 taatctatga caaccgttta tacgttggtg agttggttgg ccattctggg atactggttg
     1921 ctcattgcag gcgtaacttt acgcattcta atgaaacgac gcgcagttcc ctccgcgatg
     1981 gcctggctgt tgattattta cattctgccg ttagtcggaa ttattgccta tcttgccgtt
     2041 ggcgagctcc atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg gccttccacc
     2101 gcaaaatggc ttaacgacct taaagcctgt aagcatatct tcgccgaaga aaatagcagt
     2161 gtcgctgcgc cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg ggtcaaaggg
     2221 aatcagctac aactgatgac cgagtcagat gatgtgatgc aggcgttaat ccgcgacatc
     2281 cagctcgcgc gccataatat tgagatggtg ttttatatct ggcagcccgg cggcatggcg
     2341 gaccaggtgg ctgaatcatt aatggcggct gcacggcgtg gtattcattg ccgattgatg
     2401 ctcgactccg ccgggagtgt ggcttttttc cgcagcccgt ggcccgagct aatgcgtaat
     2461 gccggtattg aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt tttacgccgt
     2521 atggacctgc gccaacatcg caagatgatc atgatcgata attacatcgc gtacaccggc
     2581 agcatgaata tggtcgatcc tcgctacttc aaacaagatg cgggcgtagg gcaatggatt
     2641 gatctgatgg cgcgtatgga aggccccatc gccaccgcga tggggattat ttattcctgc
     2701 gactgggaga ttgaaaccgg aaaacgtatt ctgccgccac caccagatgt caatattatg
     2761 ccgtttgaac aggccagcgg tcacaccatt cacacaattg cttctggccc cggctttccg
     2821 gaagatctca ttcaccaggc attattgact gcggcttatt cggcgcgtga atatttgatc
     2881 atgacaacgc cctactttgt gccaagcgat gatttacttc atgcgatttg cacggcggcg
     2941 cagcgcgggg tggatgtcag tattatcctt ccgcgaaaaa atgactcgat gctggtcggc
     3001 tgggccagtc gcgcattctt tacggaactg cttgctgctg gggttaaaat ttatcagttt
     3061 gaaggcgggt tactgcatac caagagcgtg ctggtcgatg gcgaactaag tctggttggc
     3121 accgttaacc ttgatatgcg tagtctgtgg ctgaatttcg agattaccct ggcaatcgac
     3181 gataaaggtt ttggtgcaga ccttgccgcc gttcaggacg attatatttc gcgttcacgt
     3241 ctgctcgatg cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc cgagcgactg
     3301 ttttacttct tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac aggtagtgac
     3361 tatggatatg gatctaaaca atcgcctgac tgaagatgaa acgcttgagc aggcctacga
     3421 tatttttctc gaactggctg ccgacaatct cgacccagct gatgttctgc tgttcaatct
     3481 tcagtttgaa gagcgcggcg gcgcggagtt attcgatccg gcggaagact ggcaggaaca
     3541 tgttgatttc gatttgaacc cggacttttt tgccgaagtg gtgattggtc tggcggacag
     3601 tgaggatggt gaaatcaacg atattttcgc ccgcattctg ttatgccgcg aaaaagatca
     3661 caaactttgc catatcatct ggcgggaata aataatcgct ttcggcgtga aaaagggctg
     3721 acaactgtca gcccttattg tgtttaaagc ggatcgactt tcaggcaaga aacggcgtgt
     3781 ctgaaactcc cttccagcac aggacgtgtt ttggcgcact ccggaccggc aatcgggcaa
     3841 caggtacgga aaacacaacc ggaaggcggg ttgatcggcg acggtaattc cccttccagt
     3901 aactggatgg ttttgttctt ctccagatcc ggatcaggta tggggactgc cgacatcaat
     3961 gccctggtgt aaggatgtag tggattgtgg tagacctcat cataggtccc cagttctacc
     4021 gcatggccga gatacatcac caacacacga tcggaaatgt gttttaccac ggccaggtca
     4081 tgagcgataa aaattaatga caatcccatc tcacgttgca gctgctggag caggttgacc
     4141 acctgcgcct gaattgacac gtccagcgcc gaaatcggct catcgcagat aatcagcttc
     4201 ggttcaagaa taagagcacg agcaatcccg atacgctggc actgcccacc ggagaactca
     4261 tgcggatagc ggttaatcag gttaggcaat aacccgactt tcagcatcat cgccttcacg
     4321 cgctcgcgaa cttcctggcg tgacattttc ggatgataag tacgcagtgg ttcagcgatg
     4381 atctcgccga tggtcatacg cgggtttagc gatgccaacg gatcctggaa aatcatctga
     4441 atatcactgc gaacggcacg ccattcatcg ggcttcatgc ccagcaactc tttacctaac
     4501 caggcaacat gaccgccggt cgctttaacc aggccaatga tggcgcgagc aaaggtggac
     4561 ttaccgcatc ccgattcccc taccacacct aatgtttccc cttcatacag gcgaagcgtg
     4621 acaccatcga cggctttgag agttttcggc ggttgccaga accactgttt gccatctttg
     4681 atttcaaagt gcactttcag atcggcgatt tcgaggagga cttttcttcc ttcggttaca
     4741 gcattcataa cagttcctcc accggtttaa agcaagcacg cagacggcca ggcgtaaact
     4801 cttccagcgg cggtgcgcta ctacaaattt ccatcgcatg cggacaacgt ggctggaacg
     4861 ggcaaccttt cggtaatcgc agcaggtttg gcggattacc agggatggtc aacattgttt
     4921 caccttccgc atcgagacgc ggcaccgcgt tgagcaaacc gatagaataa ggatgaacgg
     4981 gttgataaaa gacatcgcgc gcgttgccat attccatcgt gcgcccggcg tacattacca
     5041 gcactttgtc gcagatcccc gccaccacgc caagatcgtg ggtaatcata atgatggcgg
     5101 tattaaattc ccgcttcagt tcatttaata gcgtcatgat ctgcgcctgt acggtgacgt
     5161 ccagcgcagt agtgggttca tccgcaatca gcagcttagg tcgacatagc aatgccatcg
     5221 caatcataac tcgctgacgc atgccaccag aaaattcgtg cgggtacatt ttcatgcgtt
     5281 tacgtgcttc cggcattttt accgcatcga gcatccgcac cgactcttca aacgcctcag
     5341 ctttgctcat gttcttatgc agcatcagca cttccatcaa ctgctcaccg acgcgcatat
     5401 agggattcaa cgaagtcatt gggtcctgaa aaatcattga gatttgttca gcgcgcagtt
     5461 tattgagttc acgctctggc aaattgagga tttcacgccc attgaaggtt gccgatccgc
     5521 caatacgtcc gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc gatttacccg
     5581 aaccagactc acctacaatg cccagcgttt ctccggcacg tagagaaaaa ttcaaatcat
     5641 tgaccgccgt gacatcgccg tccggggtac taaaggtgac acgcaaatct ttcacgttca
     5701 gcagtacgtc agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt acgctcatgg
     5761 ctgcactcct taacgatctt tcgggtcgag agcatcacgc aagccatcgc cgataaagtt
     5821 gaaacaaaac agcgtcacca cgaggaatcc cgctgggaac aacaataacc atggagagac
     5881 ttccatcgag ttcgcgccat cactcagcaa tgcaccccag ctgcttaacg gctcttgcgt
     5941 acccaacccc aggaagctaa ggaaagattc aaagaggatc atgctgggca ccagaagtga
     6001 tgcgtagacc accaccacac cgagtacatt cggcacaatg tggcgaataa caatgcccga
     6061 tgtcgataca ccgccaactt gtgccgcctc aataaactct ttgcgcttca gactcagggt
     6121 ttgtccacgc acaatacgag ccatatccag ccaggaaacc atgccaatcg ccacgaaaat
     6181 cagcaggatg ttttgaccga aaaaggtcac aagcaaaatg acgaagaaca tgaatgggaa
     6241 ggagttgagg atttccagca gacgcatcat taccgaatcc actttaccgc ccagataacc
     6301 ggaaagcgaa ccgtaaagtg tccccacgac cactgccacc agtgccgcag caacaccgac
     6361 catgagtgag atacgcccgc caatcgcaac gcgcacaagc aggtcgcgac cggatgagtc
     6421 agtaccaaag tagtgaccgg actccatatc cggggcgctg gacatcatcg cccagtcagt
     6481 atcgtcatag gcaaactgcg aaagcatcgg tgccaggatt acaaataacg cgatcagcac
     6541 cagcactatc agactggcaa ccgccgcacg gttatgcata aaacgtcgac gtgcgtcctg
     6601 ccacaagctg cgcccttcga cctccagctt ttcactgaaa ttttccagcg tctcgctgtt
     6661 tttcttactt aacatcatcg cgagctccag tatcagtaac ggattttcgg gtcgataacc
     6721 gcatatagca catcgacaat ggcattaaac aaaatggtta aagcaccaac caggatggtc
     6781 aggcttaaca ctaaggaata gtcacggttc aatgcgccat taacgaacag ttgtccaatc
     6841 cccggcaaac cataaatggt ttcgataacc atagaaccgg taataatgcc gacaaatgcg
     6901 gggcccatat aagagagcac gggtaacaga gcaggtttta atgcgtggcg taaaatgatc
     6961 cgccgcatag gtaacccttt cgcccgggca gtacgaataa agttggagtg taatacttca
     7021 atcatagagc ctcgggtaat acgcgcaata ctggcgatat aagcgagtga caacgccacc
     7081 atcggcaata tcatgaattt aagcgcccca ccattccagc caccgcccgg cagccaatgc
     7141 aaaatgatcg caaatatcat gactaataat ggcgcaacca caaaactggg gataacaacc
     7201 ccggtcattg ccagccccat cacggtatag tcccatttgg tgttttgttt taatgcgaca
     7261 ataacgccag cactaacacc cagtattacc gcaaggaaaa atgcggcggc tcccagtttg
     7321 gcagaaacgg gaaaactgga tgcaaccagg tcattgaccg aataatcttt atatttaaac
     7381 gatggaccga aatcaccgtg cgccagttgt ttcaggtagc tgaaatactg tgtcatgatc
     7441 ggatcattaa gatgatattt cgcttcgata ttggccatca cttccggcgg taaagtacgt
     7501 tcgccggtaa aagggcttcc cggcgcgagg cgcatcataa agaacgaaat agtaataaga
     7561 ataaatagcg tcggaatcgc ttccagacag cgacgtagaa taaattttaa cattgcccta
     7621 ccttctggcc tgtgccttct gtaatgcgat aaaaatcaga caccgtggag caggacactc
     7681 ctgccccacg tattgccatt agtgcttcac aatgtacata ttccgggtgt aggtattatc
     7741 cagcggatct ttgccggtat agccaccaac ccacggtttc accagacgcg cattcacgta
     7801 gtaataaaca ggaacaatgg ccgaatcctt atccagctgt tgttctgctt tagtgtacag
     7861 agctgtgcgc tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc tgtcaaaggc
     7921 cgggctctta taatgcgcgg tattcatcga gctgttcgac agcatggtgt tcaggaagga
     7981 agttggttcg ttgtagtctg cacaccagcc tgcacgggcc acatcaaaag tgccctggtg
     8041 acgggtgtcg aggaacgttt tccactcctg gttaaccagt ttgacgttta caccaatgtt
     8101 tttcttccac aatgaagagg cagcaatcgc cagcttttta tgcagatcag aggtgttata
     8161 cagcaggttg atggtcaacg gtttgtctgc ggtataaccc gcttcagcca gcagtttttt
     8221 cgcttcttcg ttacgttttt cctggctcca gccaaaccat tctggctgag tcaattttgc
     8281 gccatcagta tacggtggag tgtaaccata ggcgggcatg tcgccctgcg ctttcacttt
     8341 attaacaatg atatcgcggt ccatacctag tttcagtgcg gtacgcacac gcacatcgtt
     8401 gaatggcggt ttctggttgt taatttcgta atagtaagtg cacaggtatg gatcaacgtg
     8461 aacttcgtcc gggatctctt ttttcagctt ctggaacaat tcgatcggca tgttgttata
     8521 cgtcatgtcg atttcaccac tacggtagcg gttgacatcg gtaacttcag aagcaatagg
     8581 caaataggtt acctggttaa taacggtttt cgcgttgttc cagtaggtcg ggctgcgttc
     8641 aagaacgatt cgttcgttta cgacccaatc ttttaaggta taggcaccgt tggtgacgat
     8701 attaccaggc tgggtccatt tttcgccgaa tttctcgata gcggcttttg gcaccggtga
     8761 agttgatggg tgaacaagta atttatagaa gtacggaacg ggttcactta aggtgacttc
     8821 taatgtgtga tcatcaatag ctttcacgcc gagatcggta atcggttttt tcccttcaag
     8881 aatttcatca ataccggcga tatgcccata ttgcagataa ctggcatacg gagaagcagt
     8941 gttcggatca acagaacgtt gccagctata cacaaagtct tgtgctgtga ctggcgtgct
     9001 atcagaccat ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt ctttattatc
     9061 ccaggattca gcgacgccag gtgctggatg accgtcaaga tcgctgacca gtaagccttc
     9121 aaacaggtct cggctgatat tagactccgg aacaccttca attttgtgcg gatctaatga
     9181 ctgaacttct gaaccattgt tacgtaccag tgtttgtttt tccgccagtg tgacgcctgc
     9241 gggtacatca gctgccagcg cgacattccc tgccattagc gcagccagaa cgccagctgc
     9301 tactaaactt ctcttggtga tgttggtcat tgttttttgg actccctcat tataattact
     9361 ggtattaacc agcatgtgta atcccggatt gggaccctgc acaggctcgg agactttctg
     9421 ctgtcaggtt actttatcgt tacgtattac tatcaccgac tttatttatt ctggtgattc
     9481 agacgatcac cttatgtcga ttcgtactgt gtctcccctg tcgagacaca ttattctttc
     9541 gcgacgctct ttaattgtta attattctca ttaccaataa agaatcgtct ggcggttgcc
     9601 cggaaagtac caaatgcggt tcttactcgc caatacattt tgcaaaattg ttaagcaatt
     9661 ctcttttatt gaccgtatca gaatctatag cttgcatcca tgccttaaca aacatctttc
     9721 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
     9781 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
     9841 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
     9901 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
     9961 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
    10021 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
    10081 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
    10141 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
    10201 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
    10261 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
    10321 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatgctttc acgtaccgcg
    10381 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
    10441 atcgctatcg tcaccaccag gatgccccct gcgatacgga acgaatcaat tgatatacca
    10501 aaaagttgca gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
    10561 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
    10621 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
    10681 aagtaaacgg gaaaatcaaa aaaggtctga atcacggtta gctccgaagc aaaagccgga
    10741 taatgttagc cataaattaa ggttgaaaag acgcgctgac aatacgcctt ttgacagcat
    10801 ttttcacctc ctaactactt aaaattgcta tcattcgtta ttgttatcta gttgtgcaaa
    10861 acatgctaat gtagccacca aatcatacta caatttatta actgttagct ataatggcga
    10921 aaagcgatgc tgaaaggtgt cagcttagca aaaatttgat ttggatcacg taatcagtac
    10981 ccagaagtga gtaatcttgc ttacgccacc tagaagtgac gcattagaga taataactct
    11041 aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt
    11101 cataggttaa gcaaatcatc accgcactga ctatactctc gtattcgagc agatgattta
    11161 ctaaaaaagt ttaacattat caggagagca ttatggctgt tactaatgtc gctgaactta
    11221 acgcactcgt agagcgtgta aaaaaagccc agcgtgaata tgccagtttc actcaagagc
    11281 aagtagacaa aatcttccgc gccgccgctc tggctgctgc agatgctcga atcccactcg
    11341 cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga agataaagtg atcaaaaacc
    11401 actttgcttc tgaatatatc tacaacgcct ataaagatga aaaaacctgt ggtgttctgt
    11461 ctgaagacga cacttttggt accatcacta tcgctgaacc aatcggtatt atttgcggta
    11521 tcgttccgac cactaacccg acttcaactg ctatcttcaa atcgctgatc agtctgaaga
    11581 cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa agatgccacc aacaaagcgg
    11641 ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc gaaagatctg atcggctgga
    11701 tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca ccacccagac atcaacctga
    11761 tcctcgcgac tggtggtccg ggcatggtta aagccgcata cagctccggt aaaccagcta
    11821 tcggtgtagg cgcgggcaac actccagttg ttatcgatga aactgctgat atcaaacgtg
    11881 cagttgcatc tgtactgatg tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac
    11941 agtctgttgt tgttgttgac tctgtttatg acgctgtacg tgaacgtttt gcaacccacg
    12001 gcggctatct gttgcagggt aaagagctga aagctgttca ggatgttatc ctgaaaaacg
    12061 gtgcgctgaa cgcggctatc gttggtcagc cagcctataa aattgctgaa ctggcaggct
    12121 tctctgtacc agaaaacacc aagattctga tcggtgaagt gaccgttgtt gatgaaagcg
    12181 aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat gtaccgcgct aaagatttcg
    12241 aagacgcggt agaaaaagca gagaaactgg ttgctatggg cggtatcggt catacctctt
    12301 gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga
    12361 aaacggctcg tatcctgatt aacaccccag cgtctcaggg tggtatcggt gacctgtata
    12421 acttcaaact cgcaccttcc ctgactctgg gttgtggttc ctgggggggt aactccatct
    12481 ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac cgttgctaag cgagctgaaa
    12541 acatgttgtg gcacaaactt ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg
    12601 cgctggatga agtgattact gatggccaca aacgtgcgct catcgtgact gaccgcttcc
    12661 tgttcaacaa tggttatgct gatcagatca cttccgtact gaaagcagca ggcgttgaaa
    12721 ctgaagtctt cttcgaagta gaagcggacc cgaccctgag catcgttcgt aaaggtgcag
    12781 aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct gggtggtggt tccccgatgg
    12841 acgccgcgaa gattatgtgg gttatgtacg aacatccgga aactcacttc gaagagctgg
    12901 cgctgcgctt tatggatatc cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag
    12961 cgaaaatgat cgctgtcacc accacttctg gtacaggttc tgaagtcact ccgtttgcgg
    13021 ttgtaactga cgacgctact ggtcagaaat atccgctggc agactatgcg ctgactccgg
    13081 atatggcgat tgtcgacgcc aacctggtta tggacatgcc gaagtccctg tgtgctttcg
    13141 gtggtctgga cgcagtaact cacgccatgg aagcttatgt ttctgtactg gcatctgagt
    13201 tctctgatgg tcaggctctg caggcactga aactgctgaa agaatatctg ccagcgtcct
    13261 accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt tcacagtgca gcaactatcg
    13321 cgggtatcgc atttgcgaac gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg
    13381 gttcccagtt ccatattccg cacggtctgg caaacgccct gctgatttgt aacgttattc
    13441 gctacaatgc gaacgacaac ccgaccaagc agactgcatt cagccagtat gaccgtccgc
    13501 aggctcgccg tcgttatgct gaaattgccg accacttggg tctgagcgca ccgggcgacc
    13561 gtactgctgc taagatcgag aaactgctgg catggctgga aacgctgaaa gctgaactgg
    13621 gtattccgaa atctatccgt gaagctggcg ttcaggaagc agacttcctg gcgaacgtgg
    13681 ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg cgctaacccg cgttacccgc
    13741 tgatctccga gctgaaacag atcctgctgg atacctacta cggtcgtgat tatgtagaag
    13801 gtgaaactgc agcgaaaaaa gaagccgctc cggctaaagc tgagaaaaaa gcgaaaaaat
    13861 ccgcttaatc agtagcgctg tctggcaata taaacggccc cttc
//
LOCUS       DAJVYM010000107        13778 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVYM010000107 DAJVYM010000000
VERSION     DAJVYM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.108"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OQS60_002864"
     CDS             complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OQS60_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS2388565.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNI"
     gene            1102..1665
                     /gene="ahpC"
                     /locus_tag="OQS60_002865"
     CDS             1102..1665
                     /gene="ahpC"
                     /locus_tag="OQS60_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS2388566.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1836..3401
                     /gene="ahpF"
                     /locus_tag="OQS60_002866"
     CDS             1836..3401
                     /gene="ahpF"
                     /locus_tag="OQS60_002866"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS2388567.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OQS60_002867"
     CDS             complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OQS60_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS2388568.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4171..5409
                     /locus_tag="OQS60_002868"
     CDS             4171..5409
                     /locus_tag="OQS60_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS2388569.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OQS60_002869"
     CDS             complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OQS60_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS2388570.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OQS60_002870"
     CDS             complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OQS60_002870"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS2388571.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OQS60_002871"
     CDS             complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OQS60_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS2388572.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OQS60_002872"
     CDS             complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OQS60_002872"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS2388573.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OQS60_002873"
     CDS             complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OQS60_002873"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS2388574.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OQS60_002874"
     CDS             complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OQS60_002874"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS2388575.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OQS60_002875"
     CDS             complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OQS60_002875"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS2388576.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OQS60_002876"
     CDS             complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OQS60_002876"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS2388577.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12881..>13778)
                     /gene="citC"
                     /locus_tag="OQS60_002877"
     CDS             complement(12881..>13778)
                     /gene="citC"
                     /locus_tag="OQS60_002877"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS2388578.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3379 a         3491 c         3670 g         3238 t
ORIGIN      
        1 tgatattaag cgttttctga tcgggcaacc ccacggcctg ttgcagcgta ttttccttac
       61 tcatgtaata gatagccggc gtgacatttg cccccagatc gtccatcagt ttctcattgt
      121 cacttaacac tttcatttgc tctgtactta cgtttgcagg cacgtttagc ttaagcttgc
      181 caccagaggc ttcatattgt tgccaggttt ttgcgggatc tttggaggca agaattgccg
      241 ctgctgtcgc cgggctttct ggcttgataa ccccaaccaa caatgttctt aattgcactt
      301 tgccagaatc tacccacggg cgcgcctgct gccagaactg tttacaatat gggcagaacg
      361 gatcggcgaa gacgtagaca atgaccggcg catctttttt accgtcgagg atccagtggg
      421 attgttccat ccgttgccac atttcgcgtc cggctggtgc gtaaatttct ttttcgataa
      481 gtgtgttact caggttttca cctttctcgt tgtacatgta accagagata gcgtgcttac
      541 catcgggagt caggtagatg gtgacgccca tatcctgata ctttccgaga taacctttca
      601 ttcctccggg ggcatcgaac gttttgatga ttgtaatgcc ctgtttttca atcgctttta
      661 ctggggaagg aagttcctct gcgaaggcga ttgcaggaag cagagccagt aaaagtatct
      721 tttttaacat taatttgtcc ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta
      781 tcactgtcat aggtaagagc ttagatcagg tgattgccct ttgtttatga gggtgttgta
      841 atccatgtcg ttgttgcatt tgtaagggca acacctcagc ctgcaggcag gcgctgaaga
      901 taccaaaggg tagttcagat tacacggtca cctggaaagg gggccatttt actttttatc
      961 gccgctggcg gtgcaaagtt cacaaagttg tcttacgaag gttgtaaggt aaaacttatc
     1021 gatttgataa tggaaacgca ttagccgaat cggcaaaaat tggttacctt acatctcatc
     1081 gaaaacacgg aggaagtata gatgtccttg attaacacca aaattaaacc ttttaaaaac
     1141 caggcattca aaaacggcga attcatcgaa atcaccgaaa aagataccga aggccgctgg
     1201 agcgtcttct tcttctaccc ggctgacttt actttcgtat gcccgaccga actgggtgac
     1261 gttgctgacc actacgaaga actgcagaaa ctgggcgtag acgtatacgc agtatctacc
     1321 gatactcact tcacccacaa agcatggcac agcagctctg aaaccatcgc taaaatcaaa
     1381 tatgcgatga tcggcgaccc gactggcgcc ctgacccgta acttcgacaa catgcgtgaa
     1441 gatgaaggtc tggctgaccg tgcgaccttc gttgttgacc cgcagggtat catccaggca
     1501 atcgaagtta ccgctgaagg cattggccgt gacgcgtctg atctgctgcg taaaatcaaa
     1561 gcagctcagt acgtagcttc tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt
     1621 gaagcaactc tggctccgtc tctggacctg gttggtaaaa tctaaatttc cttcgtcttt
     1681 cgcgctacca gctccggcgc gaaatacttc ggaaattcac ctaattcttc gggtgctgcg
     1741 gcacccgatt ttcttccccg caccatgatg caagctgcat ccaggtagcc gtagaggccg
     1801 cttgcatgat gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc
     1861 aactcaaggc ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg
     1921 acagcgctaa atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca
     1981 aagtcacctt taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca
     2041 acccaggttc caaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct
     2101 cgctggtact ggcgttgctg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc
     2161 tggagcagat tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt
     2221 gccataactg cccggacgtg gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca
     2281 tcaagcacac tgcaattgac ggcggcacct tccagaacga aatcaccgat cgcaacgtga
     2341 tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg tcagggccgc atgacgttga
     2401 ctgaaatcgt tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca
     2461 agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa
     2521 tttactccgc acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga
     2581 tcctcgatac cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc
     2641 tggcaggcgc actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga
     2701 gcgccagcaa actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt
     2761 ctggcgcggt actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca
     2821 tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg
     2881 acggcccgct gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg
     2941 aagcggcaat tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag
     3001 aaatgaaagc cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta
     3061 ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat
     3121 atcgagatcg tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga
     3181 ttggtctgct gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg
     3241 agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact
     3301 gtacgacggt tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctt
     3361 tgagtgcttt tgactacctg attcgcacca aaactgcata agaagaagta agattcacct
     3421 gcaattgctt agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg
     3481 ccgggatgtt ccaggcaggg cggcataaat acttttatct gttagcgaat gaccagtaca
     3541 ggcagattgg cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc
     3601 gatgggttgc gagaaccgat aactacaaca tcagccccca gttcttctgc caactcattg
     3661 acttcatccc gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg
     3721 aagtggctga ccatcgtttg cagacgttct tctgcttcat gttgcagatg ctcttcaaaa
     3781 cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga
     3841 agtagatgaa taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg
     3901 ctcaattcca tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct
     3961 ccctgttaat catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc
     4021 tggttctttt tccttacgaa cggtttctgt gattaatata ttctcactga acaccaggaa
     4081 ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg
     4141 actctttttt gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat
     4201 cacgttcagg tagaaaatgt tcccgatccg ggcattgaac aggctgatga tattattctg
     4261 cgtattacgg caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc
     4321 caggttaaac atggcgatat tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga
     4381 aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt
     4441 ggcgactgtt ttttctgtcg aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt
     4501 aaaggcgctg cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt
     4561 cacctgtatg gtggcgttcc tggtggacag gcggaatatg tccgcgtccc taaagggaat
     4621 gtggggccgt ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat
     4681 attctgccaa cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt
     4741 gcagtctatg gtgccggtcc agtgggattg ttgacaatcg cctgtgcacg gttgctcggt
     4801 gcggaacaga tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc
     4861 tacggcgcga tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa
     4921 caaacggcag gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa
     4981 ggcagcacca cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg
     5041 ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac
     5101 gctggattta ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa
     5161 atgggacaga cccacgttca cgcatggctg ggtgaattat taccgttaat tgagaaagga
     5221 ttactgaaac cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga
     5281 tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg tgattctggt acccggtgca
     5341 caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga
     5401 acaatatgat cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc
     5461 cataatcaga aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga
     5521 aacggaataa ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc
     5581 gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt atcgcttgtg cagatgggat
     5641 taaagcaggt agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg
     5701 gttgcaacgc cgcccggaag ttcccagtga atggaatcgc caacgcgcag ccccagcagt
     5761 gcggcaccta ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga
     5821 tacaccagcg tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg
     5881 ttcattgtca ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac
     5941 tctgcgttta acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata
     6001 tcgatgcgtt cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca
     6061 tgtcgtcggt actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag
     6121 gcggcgaggg tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg
     6181 cgtcacattc agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcacggt
     6241 ttatggaaga cgaattagcg tgttttgtaa atcagatgat taataaccca ctttatcaat
     6301 cacaaagttt ttgccacagt tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag
     6361 cggagcgttg atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt
     6421 accctggcac gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc
     6481 ggcgtcaaag tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc
     6541 ttcgctctct ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc
     6601 aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc
     6661 agctcctggc atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg
     6721 cgatgaacac atccggctgg cgtgcgcttc tggtagattc gggattgggc gagtagcgca
     6781 accgaagcgc atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc
     6841 tggccacaga ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg
     6901 acattcatct cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca
     6961 ggagagggcc aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc
     7021 gttggcagaa gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc
     7081 tttcataatg ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa
     7141 gcccgccagg tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat
     7201 tagttccaca tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc
     7261 ccgaagattg cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata
     7321 atcaccccag gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc
     7381 agcaggatac acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata
     7441 accgacagca tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag
     7501 aacaccagaa ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc
     7561 atggtaccgg cgaaccagtc aataaagcca gaacgagtca ggccgttagc catcacaacc
     7621 agagttgcca ggttgaccag cgtgttccat gcgctgttat agcgggtaat atctttccaa
     7681 ggcacaacgt gcagggccag cattagcgaa actgccagca gaccaactgc agtagcatta
     7741 atgacttcac tgccaaaaac ccacaaacct aagctgagca atacaaggcc aatcagtgtc
     7801 cactctctgc gtgtcagcgc acccatggtt ttcagttcat cacccgccca ggttgccact
     7861 tcttcactgt gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata
     7921 agcaagataa ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata
     7981 ccggcaattt tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac
     8041 atggacgaac tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga
     8101 cgcgcggacg gatcgttggg gaatgattta aacagcggcg gcaggttttt aatgaccgga
     8161 aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg
     8221 ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa
     8281 agggcaatgc gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca
     8341 aataccagcc ataccgtggt gctggaaaaa ccagccagac cccatttcag cgcctgtttt
     8401 tgcgcattaa acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca
     8461 ataacgcaaa tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc
     8521 atgccgacaa tcattgccac aaacacagcg aagtaatgcc atgcctgtgg tggcataccg
     8581 tcggggacag ggataagaaa catgacaccc atcaccacca gtggggccaa tagtttccat
     8641 atattatctt ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca
     8701 agtgcttaaa taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc
     8761 cgcctggact gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat
     8821 cggcgggggt tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc
     8881 gcaggccccc ctcgccaccg cgcgatgcaa cgttggtatc gccgttgatc gccatcagta
     8941 ggagcaaggt atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt
     9001 aatgcggcaa ggcgtgattg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc
     9061 cggtaaggcc aagctgctgg tacaaccgtt gacctgccgt cagttgtgaa ttattggtac
     9121 gcagttctcg atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg
     9181 gcgttaccgg ttggttgagt tgaagcaaac ggccaattgc cgcacatagt agccctaaag
     9241 aaaaaatgct gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc
     9301 aagccatacc aattgggcgt aatccgtgga gtaccgcttc tggtgctatt tccgcactac
     9361 aggcaccaaa ttcaatgaaa cggggtagcc agccctgaat cgccagcgcg ctgcggtgga
     9421 aatcttccag cgccatatct ttatgtgcac cgcagttaat ccgatccacg aggcctggtt
     9481 tcggtgacag attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga
     9541 tcaatgacgt cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc
     9601 agtgcctcca tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg
     9661 gctgcgcttt gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag
     9721 agaatttcgc cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta
     9781 tgttcaagct caatggtggc gagcttgagg tcgcgagccg gggcggcaat gctcaacatg
     9841 ccctccggcc cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgtttt
     9901 gcggctaagg cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg
     9961 tctttaatcg gcccaggcgc aaccacggta aaggagacca gtggaacagg atggcgcttg
    10021 agccagacgt gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac
    10081 accgcatggt ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc
    10141 acaacatcga tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat
    10201 tcaatcggct gtggttcacc ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa
    10261 accactttaa tgcccgcttc ctgcagacgt tctgccagtt ccggacgtgc cgggttaact
    10321 gcgataccgt ggtctgtgac cagaatatcg acactggagc ctggggtgat gcaggtcagc
    10381 acgttatcca ccagagtcgg aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc
    10441 gcagaggcaa tcgcggtatc gcagtgacca ccggaagcac cacgcagtac gccgtcagag
    10501 ccggtcagca cgttaacgtt gaactgggtg tcaatttcca gcgcgctcag taccaccacg
    10561 tcgagacgat caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt
    10621 tcgatgtgat tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca
    10681 tccagcagtt tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg
    10741 ccgagggcga agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt
    10801 accgccagcg atgcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca
    10861 gagttgacaa tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc
    10921 atacgggtcg cgccagcgcc gatttttgca gcatcgccaa cgcggtcaac tttgacgatc
    10981 aaatcaacct gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg
    11041 gtaagcatca caacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg
    11101 cagcaggctt taccggtgta accgttggca ttaccgaatt catcacagga cgggacgccg
    11161 aggaaagcca cgtcgatatt cagttcgccg ctctgtacca gatgcacacg tccgccgtga
    11221 gagtggattt gcaccggttc cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg
    11281 cgcaggccgg aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagtggc
    11341 gcatggcaat cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttagcg
    11401 atgacgtcca tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa
    11461 accgtcatgc cgtcctgtaa accagagcga cgaatcgctt cttcctggtt ggcgcacagt
    11521 ttgcgatcgc gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca
    11581 agatcgcatt cagcgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca
    11641 attttctgcg tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga
    11701 gagcaccaga cgggcacgat cgataaccgg accgtccacc atcttgccgt tcagggaaac
    11761 cacgccgagg ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc
    11821 cacttctttc tgggtcggtg cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat
    11881 cagcgatttg ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc
    11941 ttcgttgtta gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc
    12001 ctgcaaaatg gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg
    12061 caggttgcgc acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg
    12121 agcgatttcc actgcgcggg taatccccag cggagattca atcgccgcca gcaggccggt
    12181 gctgccgggt tcacgaccac aggctttttc gatacgcagg atctcttttt cgatatccag
    12241 aacatcctga gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac
    12301 ggcttccagg tcgttgacac cccattcgga atccagcgcg ttgacacgca caatggtttc
    12361 aatatcgcga tacagcggat gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt
    12421 ttcacgcaat gctacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa
    12481 ggagttgctg accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg
    12541 agttttacgt tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg
    12601 ataccgctgg cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg
    12661 cctttgtcat cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga
    12721 atggtggtgc gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg
    12781 atatcctgcg tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct
    12841 gcaacggcgg gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac
    12901 gagctgccgc gtcctggcgg gagtgctcaa gcaggttctg caaataatgc agcgtgactg
    12961 cagggaccag cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg
    13021 aagcggatat cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg
    13081 gtgcggagat agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa
    13141 cgcgacaaaa gggttcagta ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt
    13201 actgacggaa aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt
    13261 taatgaagta gcaagggaac gtagcgcggg agatgatgta ttcggagccg cgatgcacag
    13321 tcaggcgtgg aatatcggcg gtgcctttta acaccagatc caaccggtct tcatagggga
    13381 agcgtgaaga atcttctttg actaaaaaca gatgcaacca gtcgcactgt gccgcagctt
    13441 gttgaatcag ataacggtga ccattcgtga agggattggc gttcatcaca atgcagccaa
    13501 tcttgttccc tggatgacga aattttttca gcgattcggc atagcgtttc agccgcgtgg
    13561 cgctgttttc catcagcacc atcacgccgg gtacgctggt cagcgtggaa aaaccgcact
    13621 ggcggaacag cgcctcgtat tcggttttgg tataaataaa cagatgcgtg ctgtgccgct
    13681 cataggcgag gtttatcaat tcagtggcta atgtcagcgc cagtccttca ccgcgaacgg
    13741 attcactgat agcaacgcat ttaataatat ttccggca
//
LOCUS       DAJVYM010000108        13260 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVYM010000108 DAJVYM010000000
VERSION     DAJVYM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.109"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OQS60_002878"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OQS60_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS2388579.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OQS60_002879"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OQS60_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS2388580.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OQS60_002880"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OQS60_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS2388581.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OQS60_002881"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OQS60_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS2388582.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OQS60_002882"
     CDS             complement(4036..5568)
                     /locus_tag="OQS60_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS2388583.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OQS60_002883"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OQS60_002883"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS2388584.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OQS60_002884"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OQS60_002884"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS2388585.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OQS60_002885"
     CDS             complement(8663..10399)
                     /locus_tag="OQS60_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS2388586.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OQS60_002886"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OQS60_002886"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS2388587.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OQS60_002887"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OQS60_002887"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS2388588.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OQS60_002888"
     CDS             complement(12121..12855)
                     /locus_tag="OQS60_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS2388589.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="OQS60_002889"
     CDS             13056..>13260
                     /locus_tag="OQS60_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS2388590.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3367 a         3370 c         3487 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caagaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAJVYM010000109        13212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVYM010000109 DAJVYM010000000
VERSION     DAJVYM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.110"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..807
                     /locus_tag="OQS60_002890"
     CDS             <1..807
                     /locus_tag="OQS60_002890"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2388591.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="OQS60_002891"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="OQS60_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS2388592.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="OQS60_002892"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="OQS60_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS2388593.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /locus_tag="OQS60_002893"
     CDS             complement(3460..6573)
                     /locus_tag="OQS60_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS2388594.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="OQS60_002894"
     CDS             complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="OQS60_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS2388595.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="OQS60_002895"
     CDS             7815..8093
                     /locus_tag="OQS60_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388596.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="OQS60_002896"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="OQS60_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS2388597.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="OQS60_002897"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="OQS60_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS2388598.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="OQS60_002898"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="OQS60_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS2388599.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="OQS60_002899"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="OQS60_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS2388600.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="OQS60_002900"
     CDS             11085..11732
                     /locus_tag="OQS60_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCS2388601.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="OQS60_002901"
     CDS             complement(11774..12304)
                     /locus_tag="OQS60_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2388602.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="OQS60_002902"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="OQS60_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS2388603.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2982 c         3030 g         3601 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc tt
//
LOCUS       DAJVYM010000110        12969 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVYM010000110 DAJVYM010000000
VERSION     DAJVYM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.111"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1230
                     /gene="adeD"
                     /locus_tag="OQS60_002903"
     CDS             <1..1230
                     /gene="adeD"
                     /locus_tag="OQS60_002903"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS2388604.1"
                     /translation="VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKEL
                     NAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCML
                     CTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGK
                     QADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASD
                     FALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACG
                     LLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSH
                     LPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQ
                     GLFDVEKFAFTTLEVTE"
     gene            complement(1275..2666)
                     /gene="uhpT"
                     /locus_tag="OQS60_002904"
     CDS             complement(1275..2666)
                     /gene="uhpT"
                     /locus_tag="OQS60_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS2388605.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(2804..4123)
                     /gene="uhpC"
                     /locus_tag="OQS60_002905"
     CDS             complement(2804..4123)
                     /gene="uhpC"
                     /locus_tag="OQS60_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS2388606.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4133..5635)
                     /gene="uhpB"
                     /locus_tag="OQS60_002906"
     CDS             complement(4133..5635)
                     /gene="uhpB"
                     /locus_tag="OQS60_002906"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS2388607.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(5635..6225)
                     /gene="uhpA"
                     /locus_tag="OQS60_002907"
     CDS             complement(5635..6225)
                     /gene="uhpA"
                     /locus_tag="OQS60_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS2388608.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6300..6590)
                     /gene="ilvN"
                     /locus_tag="OQS60_002908"
     CDS             complement(6300..6590)
                     /gene="ilvN"
                     /locus_tag="OQS60_002908"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS2388609.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(6594..8282)
                     /gene="ilvB"
                     /locus_tag="OQS60_002909"
     CDS             complement(6594..8282)
                     /gene="ilvB"
                     /locus_tag="OQS60_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS2388610.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8388..8486)
                     /gene="ivbL"
                     /locus_tag="OQS60_002910"
     CDS             complement(8388..8486)
                     /gene="ivbL"
                     /locus_tag="OQS60_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS2388611.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9051..9140
                     /gene="tisB"
                     /locus_tag="OQS60_002911"
     CDS             9051..9140
                     /gene="tisB"
                     /locus_tag="OQS60_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS2388612.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            9420..10604
                     /gene="emrD"
                     /locus_tag="OQS60_002912"
     CDS             9420..10604
                     /gene="emrD"
                     /locus_tag="OQS60_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS2388613.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(10612..11109)
                     /locus_tag="OQS60_002913"
     CDS             complement(10612..11109)
                     /locus_tag="OQS60_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS2388614.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11106..11468)
                     /locus_tag="OQS60_002914"
     CDS             complement(11106..11468)
                     /locus_tag="OQS60_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS2388615.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11458..11805)
                     /locus_tag="OQS60_002915"
     CDS             complement(11458..11805)
                     /locus_tag="OQS60_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS2388616.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            11914..12363
                     /locus_tag="OQS60_002916"
     CDS             11914..12363
                     /locus_tag="OQS60_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388617.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12612..>12969)
                     /locus_tag="OQS60_002917"
     CDS             complement(12612..>12969)
                     /locus_tag="OQS60_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2388618.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3060 a         3636 c         3392 g         2881 t
ORIGIN      
        1 gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg
       61 ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt
      121 ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga aagttatcag
      181 ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg cgaagggtcc
      241 gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc
      301 atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc
      361 ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc
      421 agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggcaag
      481 caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg
      541 aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag actggcacaa
      601 tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag cgactttgcc
      661 ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa cgaattgatt
      721 acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgtgcttt
      781 attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt gcttggcggc
      841 tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca taatattgtg
      901 gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat tcaggatggc
      961 ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt gcccattgcc
     1021 ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc cttgaaagcc
     1081 gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc gtttctttct
     1141 ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt cgagaagttt
     1201 gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg ccgggcaaaa
     1261 gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg gcggattttg
     1321 cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc ggcgatatca
     1381 agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc atcggcaatc
     1441 attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt gcctttaata
     1501 ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac accaatcaac
     1561 aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata gatatattcg
     1621 ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc gatgcaggcc
     1681 accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag cgtaccgacc
     1741 agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga gagtttcagt
     1801 tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac atagaggaaa
     1861 atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc aacaaagatc
     1921 tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat ctcctcgccg
     1981 aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc gtaacgcagg
     2041 ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc gatgacatgg
     2101 ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc accgccaagg
     2161 ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg cgtccatttg
     2221 gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct taaggcgtag
     2281 aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact gaagcccagc
     2341 atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt tttgccgtcg
     2401 gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc caggccgatc
     2461 atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg cgcgatgtta
     2521 aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag gtaggattgc
     2581 atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag ggtcggcttg
     2641 cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct gcgtgaggca
     2701 tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa aacctggaaa
     2761 atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct tcgcgcggtg
     2821 tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg atagagataa
     2881 ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc caaccagcaa
     2941 gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt
     3001 tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag acaaaaaaac
     3061 caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacaggg
     3121 agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct cggttgccgt
     3181 taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca cccagttcaa
     3241 acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg gacatataca
     3301 aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag caaaacgaaa
     3361 gcagccagat atacggattc agcaacacat atttggtgag gatctcttta cgcgtcagcc
     3421 ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca ccgaccgccg
     3481 gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt cccacgacga
     3541 tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt gcagccgctg
     3601 ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc
     3661 aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac accggtgaac
     3721 cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat agcgacgtcg
     3781 agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc ataaaataac
     3841 gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca taggtaatgt
     3901 aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta gcaaggattt
     3961 ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa cccagccaga
     4021 tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca tatttatcag
     4081 ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa ccttagacat
     4141 agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc
     4201 cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt tgcccggaac
     4261 ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca tcctgctgcc
     4321 agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc agcccttcct
     4381 ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca tcgattcgcc
     4441 attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc agtgagcgga
     4501 tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc aacaaacggc
     4561 gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc tgcttcacgc
     4621 tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata gcagtgatgg
     4681 tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc tcttcggttt
     4741 ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc gactggttaa
     4801 gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt tgcaccagca
     4861 gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc agcgcgatgg
     4921 cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc agcgcgataa
     4981 tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga
     5041 gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg cgccagttga
     5101 ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg agatagtgcc
     5161 agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc aacagcgcat
     5221 tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta
     5281 acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag cgcgagatca
     5341 gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa tgggttaaac
     5401 cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat acgggccagt
     5461 atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat aacagcaccg
     5521 ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac catgcggcag
     5581 agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca
     5641 gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt tttccatcag
     5701 attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg caatctcttt
     5761 caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg ttagcgggtc
     5821 ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc cacccgtggc
     5881 aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa gaaagccgcg
     5941 tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg agagcataat
     6001 cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat cgggcatgga
     6061 gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg cctcgcgccc
     6121 cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc
     6181 ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct tgtcctggcg
     6241 ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt
     6301 tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt acgctgcact
     6361 ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg ctggtcgtca
     6421 ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct
     6481 tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg
     6541 ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat ggcttattcc
     6601 cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca
     6661 tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg caatgcagcc
     6721 tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc
     6781 ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta gaacagactc
     6841 tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc
     6901 tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag
     6961 aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcaggcca
     7021 aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt gagcggataa
     7081 gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg
     7141 acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg
     7201 attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt
     7261 tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc ctgaatcgcc
     7321 acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttg
     7381 gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcaccgagc
     7441 acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc gtgcatcccc
     7501 agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat taaagtcatg
     7561 gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc attgatcaca
     7621 ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc tacgtcacga
     7681 atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg ctgagcttca
     7741 atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg gccagggcgg
     7801 cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc gatatgtctg
     7861 accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac ttcctggaag
     7921 gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag cgggatggag
     7981 tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc gctacaggcc
     8041 atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa gcccgcgccc
     8101 tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc atcgtaaaca
     8161 ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg ttccaggaaa
     8221 tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc cgaacttgcc
     8281 atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg aaaaaccccg
     8341 cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg
     8401 ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt tggtagtagt
     8461 tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt taacaatatt
     8521 tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg gaaaattgag
     8581 gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg agcgaggttt
     8641 cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac acagaaagta
     8701 tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt agtgcatagt
     8761 tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat acagtaaact
     8821 tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc cggcgcacta
     8881 agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt
     8941 atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct ggcaacaggc
     9001 agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg
     9061 tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg cttgatgctg
     9121 ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg ggtaaccgct
     9181 ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta tcttatccgc
     9241 cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct gccgctaaac
     9301 aactccccat tcctggcgta tatctggcta acattcatca atgtgataga ttcctctccc
     9361 gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca tccgtgataa
     9421 tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg gccgtcggtc
     9481 agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc
     9541 gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt gtctcacagc
     9601 tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc gtcggaatgt
     9661 ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg gtattgattg
     9721 ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc
     9781 cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta aacatgggga
     9841 ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg atgtggaact
     9901 ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc agtatggccc
     9961 gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt accagttata
    10021 aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt ggcggtctgg
    10081 ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg ttagggctga
    10141 gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt tttggcgcat
    10201 ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg gttatctgct
    10261 gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg aatgtctgga
    10321 cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt cctctggcga
    10381 ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg ctggtcggcg
    10441 gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg
    10501 gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg ctgtgctggc
    10561 taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac gtcaccgcag
    10621 catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca tcacccgcat
    10681 atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc tgtctggcgt
    10741 ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt cacagcgcgg
    10801 ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct caatcgagat
    10861 acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt tcccccacag
    10921 ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa gatagggaat
    10981 gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg caggttgcag
    11041 agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa tgacctgact
    11101 tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt tacagcaaac
    11161 agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat
    11221 tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta gtgccagagg
    11281 atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc cgcctggtgc
    11341 cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt acgaaaccac
    11401 gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc tttgcggcta
    11461 tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata ttaagctaat
    11521 gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt tttcattgcg
    11581 taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca acaacaacgc
    11641 cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa gcccaacccc
    11701 cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct cattagccag
    11761 cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg ctcctttgca
    11821 atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc ccgtttcctg
    11881 atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca aaataaaatc
    11941 agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat ccacttttca
    12001 ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga caaaacggct
    12061 gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac cgtggagaaa
    12121 aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact ggccattgct
    12181 gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg cattcgcggc
    12241 gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg tatcggcatc
    12301 gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg ttcgctggtt
    12361 taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt cagaacttct
    12421 gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag tcacgtacca
    12481 ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc ggccgtgcat
    12541 ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa ggacgtggcc
    12601 ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg
    12661 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
    12721 gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt
    12781 gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct tgtgtacgct
    12841 ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag ctggcggcgc
    12901 atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg
    12961 cgccccggc
//
LOCUS       DAJVYM010000111        12646 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVYM010000111 DAJVYM010000000
VERSION     DAJVYM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.112"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1041
                     /locus_tag="OQS60_002918"
     CDS             <1..1041
                     /locus_tag="OQS60_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS2388619.1"
                     /translation="PQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILT
                     RGDADYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAK
                     LAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLV
                     LISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQV
                     KPNQDMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDD
                     ANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGR
                     VIRLGEGHYQFR"
     gene            complement(1087..1680)
                     /gene="yhgN"
                     /locus_tag="OQS60_002919"
     CDS             complement(1087..1680)
                     /gene="yhgN"
                     /locus_tag="OQS60_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS2388620.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            1872..2975
                     /gene="asd"
                     /locus_tag="OQS60_002920"
     CDS             1872..2975
                     /gene="asd"
                     /locus_tag="OQS60_002920"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS2388621.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3248..5434
                     /gene="glgB"
                     /locus_tag="OQS60_002921"
     CDS             3248..5434
                     /gene="glgB"
                     /locus_tag="OQS60_002921"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS2388622.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5431..7404
                     /gene="glgX"
                     /locus_tag="OQS60_002922"
     CDS             5431..7404
                     /gene="glgX"
                     /locus_tag="OQS60_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS2388623.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7422..8717
                     /gene="glgC"
                     /locus_tag="OQS60_002923"
     CDS             7422..8717
                     /gene="glgC"
                     /locus_tag="OQS60_002923"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS2388624.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            8717..10150
                     /gene="glgA"
                     /locus_tag="OQS60_002924"
     CDS             8717..10150
                     /gene="glgA"
                     /locus_tag="OQS60_002924"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS2388625.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10169..12616
                     /gene="glgP"
                     /locus_tag="OQS60_002925"
     CDS             10169..12616
                     /gene="glgP"
                     /locus_tag="OQS60_002925"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS2388626.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         2966 a         3136 c         3489 g         3055 t
ORIGIN      
        1 ccgcaagaga aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc
       61 ttgctggcgc gtggtcatag tctggcgttg gcggtagata agtggcaacg cgccggttta
      121 tggatcttaa cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg
      181 gatgcacctc ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg
      241 gcgattgttg gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc
      301 gcgaaactgg cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa
      361 tgtgcaatgg cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc
      421 ttgttaaaaa cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg
      481 ttgatttcgc cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat
      541 aaatatattt attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga
      601 acgataaccg gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca
      661 aatcaggata tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct
      721 gaacaggctg agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga
      781 gcactccgta acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca
      841 gtaatgcccg cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc
      901 cttgcaaagg aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa
      961 caaattagtg tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa
     1021 ggtcattatc agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt
     1081 tgaaccttac cccttcatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat
     1141 caccagaatc aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg
     1201 taaaaatagc gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc
     1261 aatcaccaga tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag
     1321 aatagtcggc ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc
     1381 cggaagcccg ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa
     1441 cagaatgatg ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat
     1501 tttctcaccc gcaaacagga acaccagcat caccaggaga gcaataagca actctcgcac
     1561 catgattgcc cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag
     1621 gtttccgagc ggatccataa tcaggatcaa taaaactgct gcagaaatga ttgcattcat
     1681 aactcaaatt ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc
     1741 gcattactga tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg
     1801 tatggtgaaa gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg
     1861 aaaaaacgct tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc
     1921 tcatgcaacg catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta
     1981 cttctcagct tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg
     2041 cctttgatct ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt
     2101 ataccaacga aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg
     2161 cagcatcgtc tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg
     2221 acgtcattac cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg
     2281 taagcctgat gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt
     2341 ccgttgcaac ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa
     2401 cccagatggg ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta
     2461 ttctcgatat cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata
     2521 actttggcgt gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg
     2581 gtcagagccg cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt
     2641 ccgtaattcc ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg
     2701 cattcactat taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg
     2761 cgcacaatcc gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa
     2821 ccccagctgc cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata
     2881 tgggaccaga gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg
     2941 agccgctgcg tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc
     3001 gatgccgccc ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg
     3061 gagttaaata gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc
     3121 ttacctgaag tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa
     3181 aaaatgtcac aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa
     3241 gacaagcatg tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt
     3301 tgcggatcct ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc
     3361 ccttttaccc gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc
     3421 aaaactggag tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa
     3481 ttttttccgc tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc
     3541 ttaccgtttt ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca
     3601 cctgcgcccg tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac
     3661 gcgtttttct gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta
     3721 ctgggacggt cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt
     3781 tatccctggg gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa
     3841 cttgcgtctg aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc
     3901 tcttatttgc gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca
     3961 gtttgatgcg ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga
     4021 taacaatttc tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg
     4081 gatgggcttt acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg
     4141 gggttatcag ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt
     4201 ccgttatttc attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc
     4261 aggccacttc ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga
     4321 acacagcgat ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg
     4381 tcgccgtgaa gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg
     4441 tattgatgcg ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa
     4501 agagggggag tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt
     4561 cttgcgtaat accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga
     4621 ggagtctacc gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg
     4681 gtacaagtgg aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt
     4741 ttatcgtcag tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa
     4801 cttcgtcctg ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg
     4861 catgccgggc gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg
     4921 ggcattcccg ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg
     4981 gaaccatgac gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg
     5041 tgtccagcgt ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga
     5101 actggatttt gacccgtacg gctttgaatg gctagtggtg gatgacaaag aacgctcggt
     5161 gctgatcttt gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac
     5221 gccggtgccg cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat
     5281 cctcaatacc gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca
     5341 cagcgatgag attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct
     5401 ggccactatc tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct
     5461 cccctcggcg cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc
     5521 gagcgggtag agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca
     5581 gggcacagtg gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat
     5641 ggttatcgcg ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag
     5701 ttgttgattg atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg
     5761 cacgccggtc ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta
     5821 gtggtggttg atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc
     5881 accatcattt atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc
     5941 gagatccgtg gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag
     6001 ttgggcatta ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg
     6061 caacgcatgg ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat
     6121 ccggcgtatg cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca
     6181 ctgcataaag cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg
     6241 gacctcgacg gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata
     6301 agagaagacg gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat
     6361 ccggcggtgg tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc
     6421 gatggtttcc gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat
     6481 gcgccgttgt ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct
     6541 gaaccgtggg atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc
     6601 gagtggaacg atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct
     6661 ctgggggcgt ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt
     6721 ctgccgagtg ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc
     6781 gtttgcttca accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac
     6841 aacaattaca gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa
     6901 cggcggcgcg acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg
     6961 atgttactgg ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt
     7021 caggataacc aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt
     7081 accgccgcgt taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg
     7141 gaagaaggcg acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat
     7201 gagtggcaaa acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca
     7261 attaacgcca cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt
     7321 cccccattcg ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac
     7381 ggattgtgtg tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga
     7441 acgatcactt aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg
     7501 gaggacgtgg tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact
     7561 tcggcggtaa gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc
     7621 gtcgtatggg cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg
     7681 gctggtcatt cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga
     7741 gaatgaaagg ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca
     7801 ttatccgccg ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc
     7861 aagactactc gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt
     7921 gtatgccagt accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg
     7981 ataaaattat cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga
     8041 gcaaatctct ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc
     8101 tggaagaaga cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca
     8161 agatcaccga agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg
     8221 acccggatgc cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga
     8281 acctcgatct ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc
     8341 gcacctacaa tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg
     8401 ggatgaccct taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc
     8461 agtccgttct gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat
     8521 tgttaccgga agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg
     8581 cttgtgttat tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt
     8641 tctatcgttc agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc
     8701 ataaacagga gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa
     8761 aaccggcggt ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt
     8821 tgacgctcgc gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca
     8881 ggtagtatcc cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa
     8941 cggggttggc atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta
     9001 tcacgatacc aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg
     9061 ggttggggca gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc
     9121 gcacgactgg catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa
     9181 gtcggtgttt actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa
     9241 tgacatccaa ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat
     9301 ctctttcctg aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta
     9361 cgctcgcgag atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg
     9421 tcatcgtgaa gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc
     9481 agagacggac ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga
     9541 aaataagcgc cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt
     9601 tgcagtggtg agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc
     9661 gggtcttctg gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca
     9721 ggaaggtttc cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta
     9781 tcacgaagca ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg
     9841 ttttgaaccg tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt
     9901 gcggcgcacc ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga
     9961 tggcgtcgcc agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat
    10021 tcggcgtgct tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc
    10081 tatggcaatg gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg
    10141 cttgaaatag ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac
    10201 gcttagcgta gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa
    10261 ggacccggtc gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga
    10321 tcgtctcgtg gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca
    10381 ggtttactac ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc
    10441 gctaggaatt tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga
    10501 gctgattgat gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc
    10561 ttgcttcctt gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta
    10621 tgactacggt atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta
    10681 ctggctggaa tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg
    10741 ttttggcggt cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat
    10801 tctgggagtc gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct
    10861 gcgtttgtgg agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga
    10921 ctacttcgcg gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc
    10981 ggatgactcc acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc
    11041 ctcgaccatt caggacattt taagccgcca ttatcagttg cataaaacct acgataacct
    11101 ggcggataaa atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat
    11161 gatgcgtctg ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg
    11221 tcaggtcttc tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt
    11281 tgatatgctg ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta
    11341 tttcctgaaa accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc
    11401 gatcattgat gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag
    11461 ccacaaagtt aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc
    11521 cgactttgcg aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg
    11581 tcgctggctg gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg
    11641 taactggcgc accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat
    11701 ggttaatcac gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat
    11761 cgcccagcag ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg
    11821 tattcacgaa tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg
    11881 catcaaggcc gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc
    11941 ggcttcggcc tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt
    12001 gatcaacaac gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag
    12061 cgttagcctg gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc
    12121 agggacggaa gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat
    12181 cggtacgttg gacggtgcga atgtcgagat gcgggatcat gtcggtgctg acaatatctt
    12241 tatttttggt aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg
    12301 tgaatactac gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt
    12361 attcagtccg gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg
    12421 cgatcactat caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga
    12481 cgaactctac gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat
    12541 gggctacttc tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga
    12601 tccggtgaga ttgtaagttc accaataaat agaacggggc caaagg
//
LOCUS       DAJVYM010000112        12487 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVYM010000112 DAJVYM010000000
VERSION     DAJVYM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.113"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OQS60_002926"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OQS60_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS2388627.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OQS60_002927"
     CDS             433..621
                     /locus_tag="OQS60_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS2388628.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OQS60_002928"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OQS60_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCS2388629.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OQS60_002929"
     CDS             816..989
                     /locus_tag="OQS60_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS2388630.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OQS60_002930"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OQS60_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQS60_002931"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQS60_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS2388631.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OQS60_002932"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OQS60_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS2388632.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQS60_002933"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQS60_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS2388633.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OQS60_002934"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OQS60_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS2388634.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OQS60_002935"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OQS60_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQS60_002936"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQS60_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS2388635.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQS60_002937"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQS60_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS2388636.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3204 c         3269 g         3003 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtt
//
LOCUS       DAJVYM010000113        12449 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVYM010000113 DAJVYM010000000
VERSION     DAJVYM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.114"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="OQS60_002938"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="OQS60_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS2388637.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="OQS60_002939"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="OQS60_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS2388638.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="OQS60_002940"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="OQS60_002940"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS2388639.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="OQS60_002941"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="OQS60_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="OQS60_002942"
     CDS             3566..3850
                     /locus_tag="OQS60_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS2388640.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OQS60_002943"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OQS60_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS2388641.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OQS60_002944"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OQS60_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS2388642.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OQS60_002945"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OQS60_002945"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS2388643.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="OQS60_002946"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="OQS60_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS2388644.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10040..11140
                     /gene="ompC"
                     /locus_tag="OQS60_002947"
     CDS             10040..11140
                     /gene="ompC"
                     /locus_tag="OQS60_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS2388645.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11304..11786
                     /locus_tag="OQS60_002948"
                     /pseudo
     CDS             11304..11786
                     /locus_tag="OQS60_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11901..>12449
                     /locus_tag="OQS60_002949"
     CDS             11901..>12449
                     /locus_tag="OQS60_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS2388646.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHN"
BASE COUNT         3230 a         3104 c         2968 g         3147 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca atattaaaaa
     9841 ataaatttca aataccagta attcacctta tatctcattt cgacttcgat cacatatcga
     9901 taaataatat tatttcatct atttttctca ttgtttttaa tcaccattct attttaaaaa
     9961 cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata
    10021 attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt
    10081 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct
    10141 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
    10201 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
    10261 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa
    10321 caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg
    10381 tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg
    10441 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac
    10501 ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca
    10561 aggcaaaaat gaacgtactg acaacagtca tctttatggt gctgactaca cgcgtgccaa
    10621 cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
    10681 atataccaaa tccgatcgga caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc
    10741 ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta
    10801 ctttgcagct aattacgctg aaacattaaa catgaccacc tatggggatg gttatatctc
    10861 taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc
    10921 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacggcg accaggacat
    10981 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
    11041 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcggac
    11101 cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc
    11161 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
    11221 atttttgata tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat
    11281 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
    11341 ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt
    11401 tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa
    11461 ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
    11521 atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa
    11581 acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca
    11641 gcgttaataa aattaacacg ataacctttc caaaagaatc aaaactggaa aacatttata
    11701 ttgatcataa caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg
    11761 aagcgcaaaa taacaaactg acaaaaatta atatttctga tagttataaa ctgaaatttc
    11821 ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa tctctgattg
    11881 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
    11941 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
    12001 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
    12061 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
    12121 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
    12181 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
    12241 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
    12301 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
    12361 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
    12421 cagttactta ttttagtgcg gcacataat
//
LOCUS       DAJVYM010000114        12046 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVYM010000114 DAJVYM010000000
VERSION     DAJVYM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.115"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..770)
                     /gene="ychF"
                     /locus_tag="OQS60_002950"
     CDS             complement(<1..770)
                     /gene="ychF"
                     /locus_tag="OQS60_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS2388647.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFM"
     gene            complement(887..1471)
                     /gene="pth"
                     /locus_tag="OQS60_002951"
     CDS             complement(887..1471)
                     /gene="pth"
                     /locus_tag="OQS60_002951"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS2388648.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1748..2026
                     /gene="ychH"
                     /locus_tag="OQS60_002952"
     CDS             1748..2026
                     /gene="ychH"
                     /locus_tag="OQS60_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS2388649.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2081..3760)
                     /gene="dauA"
                     /locus_tag="OQS60_002953"
     CDS             complement(2081..3760)
                     /gene="dauA"
                     /locus_tag="OQS60_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS2388650.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3884..4831)
                     /gene="prs"
                     /locus_tag="OQS60_002954"
     CDS             complement(3884..4831)
                     /gene="prs"
                     /locus_tag="OQS60_002954"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS2388651.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4982..5833)
                     /gene="ispE"
                     /locus_tag="OQS60_002955"
     CDS             complement(4982..5833)
                     /gene="ispE"
                     /locus_tag="OQS60_002955"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS2388652.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5833..6456)
                     /gene="lolB"
                     /locus_tag="OQS60_002956"
     CDS             complement(5833..6456)
                     /gene="lolB"
                     /locus_tag="OQS60_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS2388653.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6670..7926
                     /gene="hemA"
                     /locus_tag="OQS60_002957"
     CDS             6670..7926
                     /gene="hemA"
                     /locus_tag="OQS60_002957"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS2388654.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7968..9050
                     /gene="prfA"
                     /locus_tag="OQS60_002958"
     CDS             7968..9050
                     /gene="prfA"
                     /locus_tag="OQS60_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS2388655.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9050..9883
                     /gene="prmC"
                     /locus_tag="OQS60_002959"
     CDS             9050..9883
                     /gene="prmC"
                     /locus_tag="OQS60_002959"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS2388656.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9880..10272
                     /locus_tag="OQS60_002960"
     CDS             9880..10272
                     /locus_tag="OQS60_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS2388657.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10276..11084
                     /locus_tag="OQS60_002961"
                     /pseudo
     CDS             10276..11084
                     /locus_tag="OQS60_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            11120..11974
                     /gene="kdsA"
                     /locus_tag="OQS60_002962"
     CDS             11120..11974
                     /gene="kdsA"
                     /locus_tag="OQS60_002962"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS2388658.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3002 a         3045 c         3160 g         2839 t
ORIGIN      
        1 tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
       61 caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg gtcaagatat
      121 gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg tttcagcgtc
      181 aggaagctca ggtaacgaat agccgctttc tcttcagcgc ttaaatccag cgcgcgcagc
      241 atacctgcgt tttccagctg gggcaggcat ttttctagga ccgccagctc agctttcgcg
      301 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg
      361 aggtctgcca gcgccagttc ggtgttgata acttcaatat cgtcagccgg gttaactttg
      421 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttcg
      481 gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
      541 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgttg cggttttacg
      601 atttcagcca gttgatccag gcgagggtca ggcattggta cgacgcctgt gttcggctca
      661 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
      721 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat gatttaaatc
      781 accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat ggaaataact
      841 ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa
      901 aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt tcagtacaac
      961 gcgccgcttc gtcgatggct tcatcaatta acttctgttc actaacaggc ggtttgccta
     1021 acacaaaacc gacaacttta tttttatcgc caggatgacc gattccgatg cgtaaacggt
     1081 gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg tgaccaccat
     1141 ggccaccgcc caatttaaat ttggcgacgc caggaggcag atccagttcg tcgtgggcca
     1201 ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg gctttgccgc
     1261 tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca agagtgactc
     1321 gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaaa cgctctgcca
     1381 gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca gcaccggggt
     1441 tcgccaggcc gacaatcaat ttaatcgtca cgttttttgt cctgagtgtg tacataactg
     1501 gcgcgtagtt tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa atgcagataa
     1561 cgtaataatt gcctgagtgg actattagaa agtcaaggtg ttcaggcgtt tatttgtaaa
     1621 gttttgttga aataagggtt gtaattgtga tcacgcccgc acataaccca cagggtgttg
     1681 tctatacttt acacataagg aagaggggta ttccctgtta caacccagaa agttccggag
     1741 gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg ggttggggct
     1801 ggtggtaatg gtggtcggcg tggggtattc aatcctcaac cagttaccac agtttaatat
     1861 gccccagtat ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca ttctctggct
     1921 ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt gggttcgtca
     1981 ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa taatgcagtt
     2041 tgctgaccag tcagtttgcg ctgactggtc aatctcgtac ttataaatcc gccatcgccg
     2101 cgcgacgatt cgggaagaac gcgaggcgtc ccgggatcgg ttgaatgcct gcgcgagcca
     2161 tagtgcgcag tggctggaat tccacgttgc acacgcgcag ttcacatcct tcgggcagac
     2221 gcttcacaaa acgctggaac gcatcaagac caccagcatc aagtaccgga acggcatccc
     2281 acttcagaat cacaatccgt ttgccttcaa gacgtgactc caggtccgtg aacaagcctt
     2341 cagcagcagc aaaaaacagc gggccaataa cgcgcagtac cagaacatcg tctggaacat
     2401 ctacgactac cggtgccagg cgagtcatac gtgcgatacg acgcataaac agcagcgatg
     2461 ccagcacgat ccccacgctg atggcaataa ccatatcaaa cagcacggtc agcgacatgc
     2521 acagcagcat gacaatgatg tcatctttcg gtgcatgacg cagcaagtcg accactttat
     2581 gcgcttcact catgttccac gccaccatca acagcagggc tgccatagcg gaaagcggca
     2641 gccaggagag cagcggtgcc agtaccagca gggcaagaat aaccagaata gagtggatca
     2701 ccgccgagat aggggaagtt gccccggcac ggacgttagc ggcagaacgc gcgatggcag
     2761 ctgtagcggt aataccacca aagaacggag cgataatatt ccccagtccc tgtccaacca
     2821 gttcgctgtt cgccttgtgt ttcgtcccgg tcataccatc cagtaccacg gcgcagagca
     2881 gagattcgat tgcgccgagc attgccattg agaatgccgc aggcagcagt gtgcgaatag
     2941 aatcccaggt tagcgtgaat tctgaattag gcagatccca cggcagcacc agttgcggca
     3001 gcagttgcgg aataccgtta ccctgagaac catcggccag gacgtagtgg aattgcgaac
     3061 cgatggtagc aacatgtccg ccgagcaggt taacaatccc catcaccgcg caaccagcca
     3121 gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg cggccaaaaa acaagaatac
     3181 ctagcgtcac aatgccaatg gcagcatcac ccacattaat ggtcggcagc gccataaata
     3241 atgcgccgac tttttgtaga taatgttccg ggacatgggc catttgcaga ccgagaaaat
     3301 ctttaatctg catggtaccg atggtgatcc cgatacccga ggtgaaacct aaggtgacgg
     3361 aaaccggaat atactcaatc aggcgaccaa agcgtgccag acccatcaga atcaaaaaga
     3421 tccccgacag caaggtcgca accagcagtc ctgccagtcc aaactgttgc gacacgggat
     3481 agagaattac cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac ccacccgtca
     3541 gagcaatgac aatccccgca acagctgcgg tatataaacc gtactggggt gccacaccac
     3601 taccaatagc caacgccatc gccagcggga tagcaataat cccgacggtt atcccggcaa
     3661 tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg tcgatcagag
     3721 cgcggaaagg catcacatgt gaggaaaata ttttgttcac aataatgttt catccgtgag
     3781 cgcatcatct gtcaactaaa tggcaggtga aggaggcata ggtcatacaa atggatatta
     3841 cagacaaaaa aacccgccgc agcgggtctt tgagcgggtt cgattagtgt tcgaacatgg
     3901 cagagatcga ttcttcgttg ctgatacgac gaatcgcttc ggccagcata cctgacaggg
     3961 tcagagtacg cacgttcggc agtgatttga tttcatcgct cagcggaatg gtatcgcaga
     4021 caacgacttc atcaattaca gagttacgca ggttgttcgc cgcgttgcca gagaagatcg
     4081 ggtgagtcgc gtacgcaaat acacgtttag caccacgttc tttcagagct tcagcagctt
     4141 tacacagcgt accgccagtg tcgatcatat catcgaccag tacgcagtca cgacctgcaa
     4201 cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg cggacgacgt ttgtcgatga
     4261 ttgccatatc ggtatcgttc agcagcttag cgatagcgcg ggcacgcaca acgccgccga
     4321 tgtccggaga aaccacaatt gggttatcca gattcagctg cagcatgtct tccagcagga
     4381 tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa accctgaatc tgttcagcgt
     4441 gcagatccac tgtcagcaca cggtcaacac cgacgctgga gaggaagtct gcaaccactt
     4501 tcgcagtgat tggtacacga gcggaacgga cgcgacggtc ctggcgcgca tagccaaagt
     4561 aggggataac agcggtgata cgacctgcgg aagcacgacg cagggcatca accataacga
     4621 ctaattccat caggttgtcg ttagtagggg cacaagtgga ctggatgatg aaaatatcac
     4681 caccgcgtac attttcgttg atttgtacac tgacttcgcc gtcgctaaag cgacctacag
     4741 cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg ttgtgctagt tccggggtgg
     4801 cgttaccagc aaaaagcttc atatcaggca cgagaagaac ctcaggcatg cgtccattgg
     4861 tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat cctttccagg cggtgtatta
     4921 aagagcgcga tgcaacgtct ggaacaaggt gacgttgtca ccgaaactca gcttgcccgg
     4981 cttaaagcat ggctctgtgc aatggggaaa gattaacacc tttcgccaca aagccattga
     5041 gccattccgg ggcttgctct agcacctggc gggcttcaga ctctgtatca aattcagcaa
     5101 agacacaggc ccctgtccca gtcaggcgcg acggggcgta ttctaacagc caggaaagca
     5161 ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc gcaatcattg ctgaattcac
     5221 attttagcaa cgtttctatt gacctttttg gcgtattgcg cgggagttca ggatctttaa
     5281 aaatcactgg tgtcggaata cttacaccag ggtgcgccac cagataccac ttctctggcg
     5341 gatccaccgg cgttagtatt tcaccaacgc cttcggcaaa cgcggcatgc ccccgaacaa
     5401 agacaggaac atctgcgccc agcgtcagcc ccatttccgc cagctcatcc atgcttagcc
     5461 cgcattgcca gagatgattt aatgccacca ggaccgtcgc ggcattggat gaaccaccgc
     5521 cgagaccgcc gcccatcggc aaacgcttgt caatgctgat attcgcaccg cttcccgtcg
     5581 gaagacgccc gctgtctgcc gcagttttca tcaacaaccg cgctgcgcga acgatcaggt
     5641 tatcttcatg ttccacgcct tcaacgggcg ttaacagacg aatatcccca tcgtcacgaa
     5701 gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa cagcgtttgc agcgtgtggt
     5761 aaccatccgc acgctgaccg gtaatgtata aaaacagatt aagttttgcc ggagagggcc
     5821 actgtgtccg cattatttca ctatccagtt atccattttt aacttgatgc gttgaccacc
     5881 gtcggtgagt tccatattgg ctggcatcgc aggttgcgtt ttggtgtcat aaccaccata
     5941 aacaaccttc cagtttttgc cattctggct gtaggtaatt tcgctcaggc ggtactggtc
     6001 gtccagtttg tagtcggttg catcacccgg taaacctaaa atccattggc gcaagctgtt
     6061 gagcggaatt ggcattccgg tcaatttgcc aatcatctct tcggcgtcat cggcggtata
     6121 acgctgacct ttattgtcga ctaactgcac gttacccggt tgagcattca gctccagttc
     6181 cgtgctgccc aatgggttag tgagcagcag acggtagcga tcctggccgg tttgctgcca
     6241 gaaaaagcgg gcgtacactt tttgttggtc agaaatataa gcgaacgcgc cgcgagtctg
     6301 atactgatta agattgcgca cgtcttgctg atgctgacgc cattgtggcg aatccgggct
     6361 tttgccagga cctttgggcg tggtaacgga acaggcagtg agcacaagag cagccagcgg
     6421 tagcaggcgg ataagacgaa aatcgggctg gggcatagtg atgacaagtc cttgagatac
     6481 gttgcagtta taacccttaa tgctagcgtt accgtccgct atcgtctatg ttcaagttgt
     6541 cttaattgcc agaatctaac ggctttcggc aattactcca aaagggggcg ctctctttta
     6601 ttgatcttac gcatcctgta tgatgcaagc agactaacca tatcaacgtt ggtattattt
     6661 cccgcagaca tgaccctttt agcactcggt atcaaccata aaacggcacc tgtatcgctg
     6721 cgagaacgtg tatcgttttc gccggataag ctcgatcagg cgcttgacag cctgcttgcg
     6781 cagccgatgg tgcagggcgg cgtggtgctg tcgacgtgca accgcacgga actttatctt
     6841 agcgttgaag agcaggataa cctgcaagag gcgttaatcc gctggctttg cgattatcac
     6901 aatcttaatg aagaagatct gcgtaaaagc ctctactggc atcaggataa cgacgcggtt
     6961 agccatttaa tgcgtgttgc cagcggcctg gattcactgg ttcttgggga gccgcagatc
     7021 ctcggtcagg ttaaaaaagc gtttgccgat tcgcaaaaag gtcatatgaa ggccagcgaa
     7081 ctggaacgca tgttccagaa atctttctct gtagcgaaac gcgttcgcac tgaaacagat
     7141 atcggtgcca gtgctgtgtc tgtcgctttt gcggcttgta cgctggcgcg gcagatcttt
     7201 gaatcgctct ctacggtcac agtgttgctg gtaggcgcgg gcgaaaccat cgagctggta
     7261 gcgcgtcatc tgcgcgaaca taaagtacag aagatgatta tcgccaaccg cactcgcgaa
     7321 cgtgcccaaa ttctggcaga tgaagttggc gcggaagtga ttgccctgag tgagatcgac
     7381 gaacgtctgc gcgaagccga tatcatcatc agttccaccg ccagcccgtt accgattatc
     7441 gggaaaggca tggtggagcg cgcattaaaa agtcgtcgta accaaccaat gctgttggtg
     7501 gatattgccg ttccgcgcga tgttgagccg gaagttggca aactggcgaa tgcttatctt
     7561 tatagtgtgg acgatctgca aagcatcatt tcgcacaacc tggcgcagcg taaagccgca
     7621 gcggttgagg cggaaactat tgtcgctcag gaaaccagcg aatttatggc gtggctgcga
     7681 gcacaaagcg ccagcgaaac cattcgcgag tatcgcagcc aggcagagca cgttcgcgat
     7741 gagttaaccg ccaaagcgtt agcggccctt gagcagggcg gcgacgcgca agccattatg
     7801 caggatctgg catggaaact gactaaccgc ttgatccatg cgccaacgaa atcacttcaa
     7861 caggccgccc gtgacgggga taacgaacgc ctgaatattc tgcgcgacag cctcgggctg
     7921 gagtagcagt acatcatttt ctttttttac agggtgcatt tacgcctatg aagccttcta
     7981 tcgttgccaa actggaagcc ctgcatgaac gccatgaaga agttcaggcg ttgctgggtg
     8041 acgcgcaaac tatcgccgac caggaacgtt ttcgcgcatt atcacgcgaa tatgcgcagt
     8101 taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt tcaggaagat atcgaaaccg
     8161 cacagatgat gctcgatgat cctgaaatgc gtgagatggc gcaggatgaa ctgcgcgaag
     8221 ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt tctgttactg ccaaaagatc
     8281 ctgatgacga acgtaacgcc ttcctcgaag tccgagccgg aaccggcggc gacgaagcgg
     8341 cgctgttcgc gggcgatctg ttccgtatgt acagccgtta tgccgaagcc cgccgctggc
     8401 gggtagaaat catgagcgcc agcgagggtg aacatggtgg ttataaagag atcatcgcca
     8461 aaattagcgg tgatggtgtg tatggtcgtc tgaaatttga atccggcggt catcgcgtgc
     8521 agcgtgttcc tgctacggaa tcgcagggtc gtattcatac ttctgcttgt accgttgcgg
     8581 taatgccaga actgcctgac gcagaactgc cggacatcaa cccagcagat ttacgcattg
     8641 atactttccg ctcgtcaggg gcgggtggtc agcacgttaa caccaccgat tcggcaattc
     8701 gtattactca cttgccgacc gggattgttg ttgaatgtca ggacgaacgt tcacaacata
     8761 aaaacaaagc taaagcactt tctgtacttg gtgctcgtat ccacgctgca gaaatggcaa
     8821 aacggcaaca ggccgaagcg tctacccgtc gtaacctgct ggggagtggc gatcgcagcg
     8881 accgtaaccg tacttacaac ttcccgcagg ggcgcgttac cgatcaccgc atcaacctga
     8941 cgctctaccg tctggatgaa gtgatggaag gtaagctgga tatgctgatt gaaccgatta
     9001 tccaggaaca tcaggccgac caactggcgg cgttgtccga gcaggaataa tggaatatca
     9061 atactggtta cgtgaagcaa taagccaact tcaggcgagc gaaagcccac gccgtgacgc
     9121 tgaaatctta ttggagtatg tcactggcag ggggcggact tttattctgg cctttggtga
     9181 aacgcagctg actgacgaac aatgtcagca acttgatgcg ctactgacac gtcgtcgcga
     9241 tggtgaaccc attgctcatt taaccggggt gcgagaattc tggtcgttgc cgttatttgt
     9301 ttcgccagcg accttaattc cgcgcccgga tacggagtgt ctggtggagc aggcactggc
     9361 gcggttgcct gaacaacctt gccgtattct cgatctcggg acgggtaccg gggcgattgc
     9421 gcttgcgctg gctagcgagc gcccggactg cgaaattatc gctgtagatc gtatgcctga
     9481 tgctgtctcc ctggcacaac gtaatgccca gcatctggcg atcaaaaata tccacattct
     9541 gcaaagcgac tggtttagcg cgctagccgg gcagcagttt gcgatgattg tcagcaatcc
     9601 gccgtatatt gacgagcagg acccacatct tcaacaaggc gatgtccgct ttgagccgct
     9661 cactgcgctg gttgcggcag acagtggaat ggcagacatc gtgcatatca tcgaacagtc
     9721 gcgtaacgcg ctggtatccg gcggctttct gcttctggaa catggctggc agcagggcga
     9781 agcggtgcga caagcattta tcctcgcggg gtatcatgac gtcgaaacct gccgtgacta
     9841 tggtgataac gagcgcgtaa cgctcggccg ctattatcaa tgacaagttt ttctacactg
     9901 cttagtgttc atcttattag tatcgcgctt tctgttgggc tattaacctt acgtttctgg
     9961 ctacgttatc agaagcatcc acaggcattt gctcgctgga cgcgcattgt gccgccggtt
    10021 gtcgatacgg tgttattgtt aagcggcatt gcgttgatgg ctaaagcgca catcctgcca
    10081 ttttccgggc aggcacagtg gctgactgaa aagctgtttg gagttatcat ttatatcgtt
    10141 ttgggtttta ttgcactcga ttatcgtcgt atgcacagtc agcaggcgcg cattattgcc
    10201 ttcccgctgg cgttggtggt gctgtacatc atcattaaac tcgccaccac aaaagtaccg
    10261 ttactggggt aagtcatgag atcgttagct gatttcgaat ttaataaagc gccattgtgc
    10321 gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt ttccctcgca agatgtttac
    10381 gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa tcagccagct tctgccttta
    10441 gaagagcagc tggaaaaact gatcgcgctg ttttacggcg actggggatt taaagcctca
    10501 cgcggcgttt atcgtctttc cgatgcatta tggctggacc aggtgttaaa gaatcgacag
    10561 gcagtgcggt atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc
    10621 tgctgccggt gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa
    10681 tttggctgat taatcctttt aacggtgaat cgttaagcga acatatgctg gacgtatggt
    10741 taaagggaaa tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata
    10801 acattgaggt aatccgcaaa ttgttggata cactcaaagc ctcgttgatg gaagaaaatc
    10861 agatggagct ggcgttacgc accagcgaag ctttattaca attcaacccg gaagatccct
    10921 atgaaattcg cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga
    10981 acgatttaag ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg
    11041 cgcaaataaa taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac
    11101 tcaagattaa ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc
    11161 aaatgacctg ccgttcgtac tgtttggtgg tatgaacgtg ctggaatctc gcgatctggc
    11221 gatgcgcatt tgcgagcact acgtaaccgt gacccagaaa ctgggtatcc cttacgtgtt
    11281 caaagcctct tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct
    11341 ggaagaaggg atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac
    11401 cgacgttcac gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt
    11461 gccggcgttt cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt
    11521 aattaacgtc aagaaaccac agtttgtcag cccgggacag atgggtaata tcgttgataa
    11581 attcaaagaa ggcggcaacg aaaaagtgat tctttgcgat cgcggtgcta acttcggcta
    11641 tgacaacctg gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc
    11701 gccggtgatt ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc
    11761 cggtggtcgt cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc
    11821 ggggctgttt attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc
    11881 gctgccgctg gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt
    11941 gaaaggtttc gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagcct
    12001 tagcgttctg cgttaagact tttttcatgg gtgccagata caaaaa
//
LOCUS       DAJVYM010000115        11876 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVYM010000115 DAJVYM010000000
VERSION     DAJVYM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.116"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1760
                     /locus_tag="OQS60_002963"
     CDS             <1..1760
                     /locus_tag="OQS60_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCS2388659.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="OQS60_002964"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="OQS60_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS2388660.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="OQS60_002965"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="OQS60_002965"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS2388661.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="OQS60_002966"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="OQS60_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS2388662.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="OQS60_002967"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="OQS60_002967"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS2388663.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8753
                     /locus_tag="OQS60_002968"
     CDS             7437..8753
                     /locus_tag="OQS60_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS2388664.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8774..9436
                     /gene="csiR"
                     /locus_tag="OQS60_002969"
     CDS             8774..9436
                     /gene="csiR"
                     /locus_tag="OQS60_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS2388665.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OQS60_002970"
     CDS             complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OQS60_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS2388666.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9970..10128)
                     /locus_tag="OQS60_002971"
     CDS             complement(9970..10128)
                     /locus_tag="OQS60_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS2388667.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10311..10610
                     /locus_tag="OQS60_002972"
     CDS             10311..10610
                     /locus_tag="OQS60_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2388668.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10620..11144
                     /gene="ygaP"
                     /locus_tag="OQS60_002973"
     CDS             10620..11144
                     /gene="ygaP"
                     /locus_tag="OQS60_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS2388669.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OQS60_002974"
     CDS             complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OQS60_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS2388670.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2964 a         2928 c         3062 g         2922 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggccctgtat accgcctcaa
     8281 ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc
     8341 gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcggcattc ttaacggtgg
     8401 tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta
     8461 tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc
     8521 gagcggaagg aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc acctggctgg
     8581 tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag
     8641 aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc
     8701 gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat
     8761 caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat
     8821 tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga catcgcgtta
     8881 tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg aacggctggt
     8941 cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga
     9001 tattttcgat gcccgcgcca atatggaagc gatgttagtg agtctggcga ttgcccgcgg
     9061 tggcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc
     9121 ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac
     9181 agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga
     9241 tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg tagaaatgct
     9301 ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc gagataccgc
     9361 gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc
     9421 gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat
     9481 ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac
     9541 cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc
     9601 tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat
     9661 tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg
     9721 tcgctttgcc gtcggcaatt tgaatgttca ctttatcagc atcaggtata ccggttttgc
     9781 tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt
     9841 cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct acctcattcg
     9901 ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg
     9961 agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc
    10021 aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac
    10081 gccgagcggc ggtagaatga tggtgatgac gattctccag aaacccatat gtactcccta
    10141 taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg
    10201 gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg ctggtatatg
    10261 ttgcattttt agtttagagc ttacttaatt tagaaaatac ttaaataaat atgactgaac
    10321 tcgcgcaatt acaggccagt gccgaacagg cagcggcctt attgaaagca atgagccatc
    10381 ctaaacgatt gctgattctg tgcatgctta gcggttcccc cggcaccagc gcgggagagc
    10441 tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgct cgtatgcggg
    10501 acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc attaaaaatg
    10561 aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta
    10621 tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt
    10681 taatcgatat tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg
    10741 ctccattatc cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc gagcaaatta
    10801 tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta gcagcgattg
    10861 ccgcccccgc agaaatattt ttactcgaag atgggattga tggctggaaa agagctggat
    10921 tgccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg cagatcgctg
    10981 cgggtggttt aatattaatc ggcgttgtac tgggttatac cgtaaatagc ggtttcttct
    11041 tattaagtgg ctttgttggt gccgggttac tgtttgcagg aatcagcggt ttttgtggaa
    11101 tggcaaggtt gttagataag atgccgtgga accaacgagc ttgagaagcg acgccggacg
    11161 cgccctagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga gagatttacc
    11221 ttctgccagc gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc aggttttagt
    11281 ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt ttttaccagc
    11341 gcgtggtgca gcagcagagc tattacccaa taactcttcc gggttaattc cgtcagcctt
    11401 catcagctcc agccaggtgc taattttttc ctgacgctca gccagttcac gctgctgctg
    11461 ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca tttcttcaag
    11521 aacgtcaatg gagaattcgc gagccatcgc acggagggtg cgaatgttat ttaaactttg
    11581 taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag tatttcgtcg
    11641 cgtagtgtaa gcgatcactt attatatttc cacaaaattt aatctattta cagtcttgcg
    11701 cgagattatt tcagctgtta ttttctgaaa ctctcagaat aatttcaaga ttatatatta
    11761 gccattttga tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat
    11821 tccgcaagcc tgacaaaacc gaagtgaata aaaatgcaat ataacagaat aaaaga
//
LOCUS       DAJVYM010000116        11810 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVYM010000116 DAJVYM010000000
VERSION     DAJVYM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.117"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..488
                     /locus_tag="OQS60_002975"
     CDS             <1..488
                     /locus_tag="OQS60_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2388671.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="OQS60_002976"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="OQS60_002976"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS2388672.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OQS60_002977"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OQS60_002977"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS2388673.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OQS60_002978"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OQS60_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS2388674.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="OQS60_002979"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="OQS60_002979"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS2388675.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OQS60_002980"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OQS60_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS2388676.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="OQS60_002981"
     CDS             complement(6839..7747)
                     /locus_tag="OQS60_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388677.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="OQS60_002982"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="OQS60_002982"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCS2388678.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="OQS60_002983"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="OQS60_002983"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS2388679.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11810
                     /locus_tag="OQS60_002984"
     CDS             10715..>11810
                     /locus_tag="OQS60_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS2388680.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
BASE COUNT         3006 a         2999 c         2928 g         2877 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctacctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagttaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgct
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg
//
LOCUS       DAJVYM010000117        11672 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVYM010000117 DAJVYM010000000
VERSION     DAJVYM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.118"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..504)
                     /locus_tag="OQS60_002985"
     CDS             complement(<1..504)
                     /locus_tag="OQS60_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS2388681.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGT"
     gene            complement(627..2273)
                     /gene="fumB"
                     /locus_tag="OQS60_002986"
     CDS             complement(627..2273)
                     /gene="fumB"
                     /locus_tag="OQS60_002986"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS2388682.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2351..3691)
                     /gene="dcuB"
                     /locus_tag="OQS60_002987"
     CDS             complement(2351..3691)
                     /gene="dcuB"
                     /locus_tag="OQS60_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS2388683.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4262..4981)
                     /gene="dcuR"
                     /locus_tag="OQS60_002988"
     CDS             complement(4262..4981)
                     /gene="dcuR"
                     /locus_tag="OQS60_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS2388684.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(4978..6609)
                     /gene="dcuS"
                     /locus_tag="OQS60_002989"
     CDS             complement(4978..6609)
                     /gene="dcuS"
                     /locus_tag="OQS60_002989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS2388685.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6790..7020
                     /locus_tag="OQS60_002990"
     CDS             6790..7020
                     /locus_tag="OQS60_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS2388686.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7032..7304
                     /locus_tag="OQS60_002991"
     CDS             7032..7304
                     /locus_tag="OQS60_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2388687.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7531..7827
                     /gene="ghoS"
                     /locus_tag="OQS60_002992"
     CDS             7531..7827
                     /gene="ghoS"
                     /locus_tag="OQS60_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS2388688.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7855..8028
                     /gene="ghoT"
                     /locus_tag="OQS60_002993"
     CDS             7855..8028
                     /gene="ghoT"
                     /locus_tag="OQS60_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCS2388689.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8147..9664)
                     /gene="lysS"
                     /locus_tag="OQS60_002994"
     CDS             complement(8147..9664)
                     /gene="lysS"
                     /locus_tag="OQS60_002994"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS2388690.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(9901..11358)
                     /gene="dtpC"
                     /locus_tag="OQS60_002995"
     CDS             complement(9901..11358)
                     /gene="dtpC"
                     /locus_tag="OQS60_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCS2388691.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11417..>11672)
                     /locus_tag="OQS60_002996"
     CDS             complement(11417..>11672)
                     /locus_tag="OQS60_002996"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS2388692.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3018 a         2826 c         2859 g         2969 t
ORIGIN      
        1 ggtgccgaga aaatcatccg ctccctggcc cattgccagc gccgcccacc actcgattaa
       61 ttcatacatc gcgcttatcg ccagcgccac gcagcagact aggaacgcca ccattttacg
      121 tccgcgcacg tacatcccgc gcacgagaat ttctcgtgcc accagtgcag gcaccagccc
      181 ctggaaaaaa tgccccagct tgtcatacgg attacggctc aaccccagcc attcctgcac
      241 ctcaaaaccg atggggactt tcgcgtaggt gtattgcccg ccgaccatca ggatgatggc
      301 gtgaaagaaa atgagcgtat agagcagcgg cgttaacgga taacgtttag cggtggcaag
      361 cagtagcggc acgacaataa tcaccggtgt cacttccatc agccaggtga gtttgtcatg
      421 ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc agtgcgccgg tgttaagaat
      481 taacggtttg agtgtgcggg tcatggtgag ttcgcaagtc agggaaaata ctgactattc
      541 accagcaagc atgaaattac aatcgcaaaa gaggtggccc agggggatca cctggcagca
      601 tgctgccagg cgctgggccg aagaggttac ttagtgcagt tcgcgcactg tttgttgacg
      661 atttgctgga agaagtcgtt acctttgtca tcgaccagga taaacgccgg gaaatcttct
      721 acttcgattt tccagatagc ttccataccc agctccggat aagcgacgca ttccagatgc
      781 ttgatgctct gctgcgccag taccgccgcc ggaccgccga tgctaccgag gtagaagccg
      841 ccgtgtttat gacacgcgtc ggtaacctgc tggctgcggt tgcctttcgc cagcatgatc
      901 atgctgccgc cgtgggattg cagcagatcc acataggagt ccatacgacc tgcggtggtt
      961 gggccaagtg aacctgatgg ataaccggca ggagttttcg ccggacccgc gtagtagatc
     1021 gggtgatctt tgatgtactg cggaagttct ttaccggcgt caatcagctc tttcagcttg
     1081 gcgtgtgcaa tatctcgacc cacaataatg gtgccagtga gcgacaaacg agtggaaacc
     1141 ggatattgcg aaagctgggc gaggatctct ttcatcgggc ggttaaggtc gaccttcacc
     1201 gcttcgcctt caccggcctg acgcagttct tgtggaatgt actgacctgg attgtgttcc
     1261 agtttttcga tccagatacc ttcgcggttg attttcgctt taatgttacg gtcagcggag
     1321 caggagacgc ccatgcctac cgggcaggat gcgccgtgac gtggcagacg gataacgcga
     1381 atgtcgtgcg cgaagtattt accgccaaac tgcgcgccaa gaccgagttt ctgggcctct
     1441 tccagcagtt cctgttccag ctggacatcg cggaacgcct gaccatgttc gttcccttcc
     1501 gtcggcagtt catcgtaata gtgagcgctt gctaatttga cggttttcag gttggtttcc
     1561 gcagacgtac cgccaatcac aaacgcgata tggtacggcg ggcaggctgc agtaccgagg
     1621 gtacgcattt tctcgacgag gaagtttttc agtttgccgg gagtcagcag ggctttggtt
     1681 tcctggtaga gatacgtttt gttggcagag ccgccgcctt tcgcaacgca aaggaatttg
     1741 tactcatcgc catctaccgc gtacaggtcg atttgcgcag gcaggttagt gccggtgttg
     1801 acctctttgt acatgtccag cgccgcgttt tgcgagtagc gcaggttatc ttcgatatag
     1861 gtgttataga cgcctttcga cagcgcttct tcatcaccgc caccggtcca cacgcgctgg
     1921 ccttttttac cgacgatgat cgcggtgccg gtatcctggc aggtcggcag cacgcctttg
     1981 gcggcgattt cggagtttct taagaattgc agtgccacgt acttgtcgtt ttcgctggct
     2041 tctggatcgt gaagaatagc cgcaacctgt ttctggtgtg ccggacggag catgaaagaa
     2101 gcgtcgtgaa aggcttgctg cgccagcagg gtcagggctt ctggttccac tttcaggatg
     2161 gtttcgccgt cgaagtcggc aacgctaacg taatcggaag tgagtagata gtattcggta
     2221 ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg gtttgtttga catagcttcc
     2281 agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc gcgtagtcac gggcggcacg
     2341 caaagtgcat ttataagaac ccgtacatcg cggcgaagat ccagccgaag acgcacgata
     2401 cgctcacacc aatcaacccc ggcagaataa agctgtggtt gatgacgaag cgaccgatgt
     2461 gggtggtgcc ggaacggtca aactgaatcg ctgccagatc gctcggataa gtcggcagga
     2521 tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta tgccggatca acgccgatcg
     2581 ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg agagtttaca aacttggaaa
     2641 ccagcagcag aacaatggca taggcccacg gatactcttt caccatttca cccagtacgc
     2701 cctgaatttc agacatatgc gcaccgaaca tggtttctgc catccatgcg ataccgtaca
     2761 ccgccacgat ggcgatcata ccggaacgga agacttcgtt ttttgagata gacgcgggat
     2821 tggttttggt caggataata atcagcgccc cggtcagcag cataaacatc tgaataacca
     2881 gtaccatcga cagcggtttg ccgccgaagg atggacgcag gtccgaatca gcaccaagaa
     2941 gggcgactac agcgattgcc ccgaggaaaa tccacattgc cagccagttg cttttcggca
     3001 gttttttgtc cagcagcgtc gcggtatcac cgtaaacata ctcacggttt tccggtacgg
     3061 agatgaattt ctggaactct tcgtctttat ccagatcttt accacggaac cagctgaaga
     3121 taccgatcgc caggataccg attaacgtcg atggaatggt gattgccagc aggtcgagga
     3181 actcaagatg gcgaccatca aaggtgacat tacccagcat tgcaaccaga gacacgaccg
     3241 caaccgacac cggactggcg atgatcccca tctgtgcacc gatagaactt gccgccatcg
     3301 gacgttccgg acggatgttg ttcttaatgg cgacgtcgta gatgatcggc agaatggtgt
     3361 aaaccacatg acccgtaccg caaagaatgg tcagcgtaca ggtcacaaac ggcgcgacaa
     3421 ttgagacata tttcgggttg cggcgcagca gcttctcggc aatttgcagc atgacatcaa
     3481 gaccgcccga agcttgcaag gtcgccgatg ccgccaccac cgcaatgata accagcatga
     3541 catcaaccgg tggtttacct ggctgaaggt ggaagacgaa gaccagaatg accagaccga
     3601 taccgcctaa taaacccagc gcgataccac cctttctggc accataaaac agacatatca
     3661 gtattatgat aagttggata gtaaataaca tgtgtgaacc ctcgcgataa tcctatttaa
     3721 atttttgctg aatagatcac agtcacgttc tgttttgtat gaactgtttt cagagcctga
     3781 ctaaatatcc gtttggtcgt taccggcttt agcaaatacc tcacagtgaa tattggctga
     3841 ttaaagcagc ggcaaattta tgcactgtga taagcggctt tttcagagag aaagactctc
     3901 tggcgatggg tttatagttt gtactgcgca gatgttaatg ttttgtttgc tgttatctta
     3961 ttgatattta tcggtctatt ttttgttctc agggcagatg tcattaggtt tatagattaa
     4021 tctgatctac ccatttgtgg gtaaaaatac acataatgcg ggtgacataa tagttaatta
     4081 acttttgtta gcgttttgaa attaaaaaca ccgttcacct gaagagatat taatttttag
     4141 cgatgatgca gggataatta tatttgatct ggcacaagtt ttactgatga aggatgtaac
     4201 ttgtgccagg ggtattttgc attacggtaa taattattac ttacagataa ccagcgatcc
     4261 gttattggca atattgtttc agtagtgagt agtgttctgc ctgaatacgg taacggtaaa
     4321 ctggacgccc cgtgacgcca taatggatac tggtgaacaa gatgtggcag ttgaccagcc
     4381 agatgaggta tttacggcag gaaacacgcg aaatgttaac ctcgttggct agctcgtcgg
     4441 ttgaaaattc atagtcctga tgcgcgtcaa tccactggca cagtgtgcgt aacgtctgcg
     4501 gcgttaagcc ttttggcaag cgacgaggat cctgttcgtt ggagctgctg ccgtggatta
     4561 gctgatcaag ctcggcctgg tcataatact gatgtttttc cagcgccatt ttcttttgcc
     4621 gccagtcggt gagcgcctct tcaaagcggg aagcctggaa gggtttgatc aggtaatcca
     4681 cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag gagatgacaa
     4741 tcacatcact tttgcaacgc gcgttatgca ggacaggcag taaatcgagc ccgttctctt
     4801 tttgcatata gatatcgagc aatatcaggt cgataggcgt atcgctattg aagataatct
     4861 ctttggcttt ctccagcgtc gaggctgttc cacagcattg aaagcctggg atttgtgcta
     4921 cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc gataattaat acattgatca
     4981 tctgttcgac ctctccccgt cccagggtat ctggacaaaa aattgtgtga aaatcccggg
     5041 ttccgattcc acggcgatgc tgccgccgag attttctacc tgttgtttga caagtgctaa
     5101 accgacgcct cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt gatcgatttt
     5161 gtcgggtgcg atccccggtc catcatcgtt aacttcgcag tgcagccagc cgtgacggta
     5221 gtgcaatgtt acgctaattt cgccaccggg ttccggccct aatgcttcca gcgcgttttc
     5281 tatcagattt cccaatgtgg taatcagcgt cgcgacctgg tcctcactgc cgctgtctgg
     5341 cagctggctt tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc gattaatctt
     5401 gctgattaaa aaaccagcga taaccggaga tttgatctta cccagcagag agccaatctc
     5461 ttcctgatag ttattggctg tttttagaat gtaatcttcc aactgcttat aactcttcag
     5521 atgcaataat ccgagaatca cgtgcaattt attcataaat tcgtgggatc gttcacgaag
     5581 tgcgtcggca tagttgacca gaccgtcgag tcgctgcatc agtttacgta cttcggtttt
     5641 gtccctgaag gttgaaatgg caccgatgat aacgccatta ctgcgcaccg gaacggtgtt
     5701 gatcagtaat agccggtctt taatcgtaat ctcttcgtcg cggcgcggcg taccgtcgcg
     5761 taacacttcc gagacatcta ccacctgtga ccatgagtgg ctgagcgtcg acagtttctc
     5821 atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac
     5881 ctcgccgcga tcgtccacgg caacgacgcc ttctttgata gactgcaaca tggcctggcg
     5941 ttgctcaaac agcgtggaga tttcgtaggg ttccaggccg aaaaggattt ttttcagtac
     6001 cttaaccaga atgcaggtgc caatcagtcc gaccagcatg ccaaataata ccgaccagat
     6061 aatgctccag cgactgtcat tgatttgttg ggtcacgcgg cttaactcaa ggccgatcgc
     6121 caccacgcca atttgtttat gattttcatc gtagatgggg gtaaatacgc gtaaagcctg
     6181 cgccagaaaa ccgcgattga tagcgacatt ttctttgcca ttcagcgctt taaggatgtc
     6241 atcaccttta aatggctgac caatacgctg ggcttcagga tgcgagtagc gaagactttg
     6301 catatcggta acgacaataa acagcagatc gttgcgtttg cgcacggctt ccgcgatggc
     6361 ctggatgccg ctctcctgcg gttttttctg caagccctga cggatttccg gcgagttggc
     6421 gagggtacgc gccactgcca gtgccttgtt ggctagcccg tctcgcgtca tatcactgat
     6481 ttgcgagaag taaatcagat gcaccaccaa tagcaccgag aacagtaccg cactgaccat
     6541 taagatcact gtggtactca atttcatcgg acgtttgcgt aacatgcggt agggcaatga
     6601 atgtctcatc agcttccttg tgtgacaaat ttcttaaaca ttatctctga tgaggtgggt
     6661 aattcaaagg gagtaagaat gattggctat ataggggaag agattctggc aacggaaact
     6721 gccagtgctg tatgaagatt ccagggctat gcttatagcg ataatcatac tgatgagaga
     6781 gggaaagtca tggatcaggc gctactggac gagggttatc gctgttatac cggcgaaaag
     6841 atcgatgtct atttcaacac tgcgatatgt cagcattctg gcaattgcgt acgtggcaac
     6901 ggcaagttat ttaatctcaa acgaaagccg tggatcatgc cggatgaagt cgacgtcgcc
     6961 actgtggtta aagtgattga tacgtgcccg agcggcgcgc tgaaataccg tcataaataa
     7021 gcgagggtaa aatggaaata cgcgaaggcc acaataaatt ttacattaat gacgaacaag
     7081 gcaagcaaat cgctgaaatt gtctttgtgc cgactggaga gaatttagcg attatcgaac
     7141 ataccgatgt cgatgaaagc ctgaaagggc aggggattgg taaacagctg gttgcaaaag
     7201 tcgtggaaaa aatgcgtcgg gaaaaacgaa aaattatccc actatgccca tttgcgaaac
     7261 acgaatttga taaaacgcgg gagtatgatg atattcgcag ttgatgggag agtacagagt
     7321 cacgatattt ttcatgctct ccgcggagtg atgcaggaga gcatctgaag ggtaggggga
     7381 tgcacaaaga atgggcagag agcgcgtttt tttgtcccaa gtcatcccct ttactgagca
     7441 aaaaaaagaa tatctcctat atgagaatca tcaatcgggg ttatcaagtt ttgcgtcccc
     7501 agagcattca atattgatag gagtcatatt atggaaggta aaaacaagtt caatacttat
     7561 gttgtttctt ttgattatcc atcatcttat tcctcagtgt tcttaaggtt aagatcattg
     7621 atgtatgata tgaatttctc ttctatcgtg gctgatgaat atgggatacc acgacaattg
     7681 aatgaaaatt ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga agatttaatc
     7741 aggcttaaat gcttagactt accggatatt gattttgacc tcaacattat gacagttgat
     7801 gactatttcc gtcagtttta caagtagcca ggaaggataa gaagaacata aaaaatggca
     7861 ctattctcta aaatattaat tttttatgtg attggtgtga acatatcctt tgtcattatc
     7921 tggtttatct cacatgagaa aacacatatt cgtttactta gtgcattcct ggtcggaata
     7981 acctggccaa tgagtctgcc tgtggcatta cttttttctc tcttttagga gctgtagttg
     8041 agtggacccg ctggttatcg tgaaacgaag cgtctctcct caaaactgga tttgcggaac
     8101 gacgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat ttctgtgggc
     8161 gcatcgccgg gaagagaata acgtcgcgaa tagtatgact gtttgtaaac agcataatca
     8221 ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc agcgcagtta
     8281 cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc agctttagcg ttaacctgtt
     8341 cctggaaacg ttcagcctga tcttctgcgt cgtttaattc gctaaaacca ttaccgattt
     8401 cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca tcattacggc
     8461 gcgccagcgg tgacacttct gccggatatt ccgtaataaa ggttggctga atcagatgtg
     8521 cttctgccac ttcatcaaag atctccgtga taatacgtcc cagcccccag cttttctcta
     8581 ccgtaattcc gatagattca gctaatgctt tagcagcatc aaaattatcc aggtcggcca
     8641 tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt tcaaacggtt
     8701 tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccaga acctcttgtg
     8761 ccagggtgcg gaacagtgac tctgtcagtt caatcaaatc gtggtaatcc gcatacgcca
     8821 tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct tcattacgga
     8881 agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt ttcagataca
     8941 gctccggcgc gatacgcaga tacatatcta aatccagagc attatgatgg gtaataaacg
     9001 ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact tccataaagc
     9061 cgcgcgcgac catgaactga cggatagcgg ccagaatttt tgaacggaca acaaacgttt
     9121 gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg acttcctgat
     9181 cctgcagacc atggaattta tctggtaaag gacgtagtgc tttagtcagc aggcgcaact
     9241 cagtacagtg aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg cgggcaccga
     9301 taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct ggcaggctat
     9361 ctcttgcaac gtacagttga atacggccac cgacatcctg caacgttaca aaggaggctt
     9421 tccccatgat acgacgggtc atcattcggc cagcaaccga gacttcaatg tttaaggatt
     9481 ccagttcctg gttattcttc gcatcaaact cttcgtgcaa ctggtcagag gtatggtcgc
     9541 ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt ttttcgcggc
     9601 gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt tcttgttcag
     9661 acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga cagatcggta
     9721 cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa ctaacgctta
     9781 cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact tattaatctc
     9841 gatttactag tgtgattaac tgaaagttaa tttattcata accaattgtt tttacgacag
     9901 ttaatcgttg ctctcctgta tcatattcgt cggcgtgcta aacagaaaac gggtagcaaa
     9961 ggccaatacg acgatgatcg cgacacaagc caacgtccac tctcccatct gagaaaagaa
    10021 tcgctggtaa gctgcaatta ccataccgct aatttgcgac tctgtcgtct gctgtgccac
    10081 gacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatac cggttaatac
    10141 gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa agagttctgc
    10201 aaagcccatt agcgccaacc cggatatcat cacgcccatt gacgcttgac cgtcagccgc
    10261 tgcatgtcgg gcatcaaatg ccaacaacat aaagccacaa gccatcagca gtaagccaaa
    10321 ggcaaacttc agccagacgc gcaatgttga gttgccgcgg ctttctggcg acgccagcca
    10381 ggccagtaca accccagcga gcatcactgc aattgcattc accgactgga atagtgctgt
    10441 aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca agctgatggt
    10501 actgccgccc tgttgtgcca gtacccagaa caacgtcccg acaaacatca acaatacaat
    10561 ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa tgatttgtgc
    10621 ggcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca gcagcagggt
    10681 aaaaaatact ggggctaaac agagcatcac cactaaccag ctccatactg gtaaggcaaa
    10741 tttgacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat gacgatgacc
    10801 gcttaagaaa atcaacaaac cgataaacat gccgccaccc gcaagggcaa agccaacatg
    10861 ccatccatac cactgagcag ccaggccgca ggcgattggg gcggcgatag aaccgatatt
    10921 gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct cgtcgtagag
    10981 ctcgccaagc aaacagctga tgtttgattt gaataaaccg tagccacaaa taatgattgc
    11041 cagcgccaga tacaggctaa aggttgaatt tgtatcaatg cccagcacca catggccaag
    11101 ggtcattaac agcgcgccgg caatcactgc agtgcggttg ccgagcaggc ggtcggcaag
    11161 ccagccgccg agaatagggg taacgtaaac cagagaagca tatgcgctga acaggctgat
    11221 ggcatggtta tcatcaaaac caagctgatg ggtgagatag agaatgagta aggcacgcat
    11281 gccgtaaaaa ctgaagtact cccagatttg gatcgccacg atatagtata tcgcgcgcgg
    11341 ctgtgagggt gttttcatgt gttctcctta tgagcaaaaa agggaagtgg caagccactt
    11401 cccttgtacg agctaattat tttttgcttt cttctttcaa taccttaacg gtatagcggc
    11461 catcagcctg acggtatgca ccgtgaatat cggtttcaaa tcccggatag tgagtgccga
    11521 tttcacacag catctgcagg aactccagaa ccggacggct ttcttcggtg atcatttcac
    11581 ccggcattac cagaggaact cccggcggat acggaaggat catattggcg ttaatacgac
    11641 ctaccatttc gtcgaggtaa acttcttcgg tc
//
LOCUS       DAJVYM010000118        11428 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVYM010000118 DAJVYM010000000
VERSION     DAJVYM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.119"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(44..385)
                     /gene="bamE"
                     /locus_tag="OQS60_002997"
     CDS             complement(44..385)
                     /gene="bamE"
                     /locus_tag="OQS60_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS2388693.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(534..2195)
                     /gene="recN"
                     /locus_tag="OQS60_002998"
     CDS             complement(534..2195)
                     /gene="recN"
                     /locus_tag="OQS60_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS2388694.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2281..3159)
                     /gene="nadK"
                     /locus_tag="OQS60_002999"
     CDS             complement(2281..3159)
                     /gene="nadK"
                     /locus_tag="OQS60_002999"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS2388695.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3282..3875
                     /gene="grpE"
                     /locus_tag="OQS60_003000"
     CDS             3282..3875
                     /gene="grpE"
                     /locus_tag="OQS60_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS2388696.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3930..5216)
                     /locus_tag="OQS60_003001"
     CDS             complement(3930..5216)
                     /locus_tag="OQS60_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS2388697.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5237..6028)
                     /locus_tag="OQS60_003002"
     CDS             complement(5237..6028)
                     /locus_tag="OQS60_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS2388698.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6195..7556
                     /gene="ffh"
                     /locus_tag="OQS60_003003"
     CDS             6195..7556
                     /gene="ffh"
                     /locus_tag="OQS60_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS2388699.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7805..8053
                     /gene="rpsP"
                     /locus_tag="OQS60_003004"
     CDS             7805..8053
                     /gene="rpsP"
                     /locus_tag="OQS60_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS2388700.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8072..8620
                     /gene="rimM"
                     /locus_tag="OQS60_003005"
     CDS             8072..8620
                     /gene="rimM"
                     /locus_tag="OQS60_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS2388701.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8651..9418
                     /gene="trmD"
                     /locus_tag="OQS60_003006"
     CDS             8651..9418
                     /gene="trmD"
                     /locus_tag="OQS60_003006"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS2388702.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9460..9807
                     /gene="rplS"
                     /locus_tag="OQS60_003007"
     CDS             9460..9807
                     /gene="rplS"
                     /locus_tag="OQS60_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS2388703.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9884..10366)
                     /locus_tag="OQS60_003008"
     CDS             complement(9884..10366)
                     /locus_tag="OQS60_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS2388704.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10382..>11428)
                     /gene="dgcN"
                     /locus_tag="OQS60_003009"
     CDS             complement(10382..>11428)
                     /gene="dgcN"
                     /locus_tag="OQS60_003009"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCS2388705.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2748 c         3061 g         2817 t
ORIGIN      
        1 tcattgagca ccttttttaa cgtctttgag agcaacttta ttattagtta ccactcagcg
       61 caggtttgtt atcaatattg gtcaacacac cgctactgtt aaaggtcagc gtcagcgttt
      121 gctgcgttac accttcatga cctggttgct ggcggaagac atagaaccag gtattcgtac
      181 caaatggatc ggacatcagc ggtgtaccca atgcgtacgc aacttgttgt tgcgtcatgc
      241 caacacgtat tttggatacg tcgttagcgg tcagatagtt cccctggttg atgtcaggac
      301 ggtaaaccac tcgctccaga gtggaacagc ctgcggtcaa catcaatagt actgctgctg
      361 cagcagtcag cgttttacag cgcatagtga tttgattcct tttcgggccc gagcagtacg
      421 tggctcatct gtaatatgcc gatgataata gacctttcat cactttaaaa ccttttgctt
      481 tccggtctta cggcgttttg ctgtttactc taaccgtgaa gcaggaaaaa agtttacgct
      541 gcaagcagtt ctttcgcatt cgctagtgta ttacgtgtga cttcactgcc accaagcagg
      601 cgcgccagct cttgtaaccg cgctttttta ttcagggatt gcatatgcgt ttctgtcatc
      661 gcaccatcgg tttctttgct gacaaaatag tgttgatgac cacatcccgc gacttgtggc
      721 aggtgggtaa cacacatcac ctgagttgat tcgccgagtt gacgcagcag tttgccgaca
      781 actgccgctg ttggaccgct aatccctaca tccacttcat caaaaatcag tgccggggtt
      841 tccattttac gcgccgtgat gacctggatt gccagtgcga tgcgggacaa ttcaccaccg
      901 gatgcgactt tggcaatagg ctgcattggc tgacctgggt tggtggttac ccgaaactca
      961 atacgatcgg caccgtcagc gcccaggtga tgctcgtcaa atttaacatc gatcgtaaac
     1021 tgcccatgcg gcattgagag cgcatgcata ctgtcggtga tcagctgtgc aagttcttct
     1081 gcatattgct ggcgttgttg gtgtaatgcg cgcgcgattt ccagtgcctg ctgatgatgt
     1141 ttcgttaccg ccagcgcaag cgtttcttgt gagtcggcct gatcgtccag ttgctgctgt
     1201 tcttccagta gcgactggta atactgtggc aatgcctcag ggctgacgtg atgtttacgt
     1261 gccagcgaaa tctgttttga gatgcgctgt tcaagttcaa atagtcggtt gggatcgaga
     1321 tccagacgat cgcagtagtg gcgcagttca tcgctggctt cagcaatctg gatggtagct
     1381 tcttccagca tatcaagtac gccggacagt ttgctgtcca tgccaatcaa ttcgctcacc
     1441 agttgtttag ccgtgtaaag ctgactttgc aggtttgcgt cttcaccgtc ggccattaat
     1501 gccaatgcat tctggctggt ggtcagcaat tgaccgctgt tcgccagacg tttgtactct
     1561 tcgtcgattt gttcaaactc tccgggctgc ggattaaatt cgttaagttc ttttaattgg
     1621 tattgcagca gttccgcacg ggcggcgcgt tcctgactta actgttgatg atgcgcgagg
     1681 tcacggcagc tttgatgcca caactgataa cgtgcggtca tttcctgcag tagagaggtt
     1741 tcattggcat agccatcaag caggaatttt tggtgctcag gtttggtgag taattgatga
     1801 gcgtgctgac catggatctg aatcagcaac tgacccagtt cgcgcagttg tgacagagga
     1861 acagctgtac cgttgatgaa accacgggag cgaccatcgc tgctgatcac gcgacgaagc
     1921 aaacattcat gcccgtcttc aagctggttt tcttccagcc agcgcagagc cgctggcgta
     1981 tctttcagag aaaaacgggc gcacaggtca gcgcgagcag cgccggtacg caccatgtcg
     2041 gcttcagcgc gaccaccgag acaaagaccg agggcatcta ttgcaataga tttacccgcg
     2101 ccggtctcgc cagttattac ggtcatgccg ctatgaaaat caatctcaag ctcacgaacg
     2161 atagcaaagt tgctgatggt cagttgtgcc aacatagtcg ctttcctgta tgaaaaacca
     2221 ttactgttat tgtgtacagt ataaactggt tttatataca gtaaagaggc tggcgtaaaa
     2281 ttagaataat ttttttgacc agccgagctt ggtgcttaat gtgttgaaat aactgtaatc
     2341 tttcggatga atcagattca gatggtaatc acagcgacga atcaggacat cttcaccttc
     2401 ctgaatcggc agtgctatct ggctgtcgca actgatttcc aggtcgttac ggcgatgcga
     2461 aaaacgcaga cggatcgtgc tgctgctgtt tatgaccagt ggtcgtgctg acaacgtatg
     2521 cgggaacatg ggcaccaggg taatcgcatc cagagagggg gtcagaatag gaccgcctgc
     2581 agagagggaa taggcggtgg agcctgttgg cgtcgaaata attagtccat cagatcgctg
     2641 agaaaacgca aagatctcgt cgatatacac ttcgaactca atcatatgcg ccactttgcc
     2701 tggatgaagc accacttcat ttatcgcggt gctgatgcgt ttctggcaat cttgctgaca
     2761 gacttgcgct tccagcaaaa aacgtttctc gctgatgtag tggccttcca gcacatcggc
     2821 taactgttgc tgggcgttat cggggtcaag gtcagtcagg aaacccaggt tgccacggtt
     2881 gattccaata actttaatat cgtaacgggc gagtgtgcgt gccgcgccca gcatattacc
     2941 gtcgccacca acgactaccg cgagatcagc tagttgccca atctccgcga gcgtgccagt
     3001 tttcacattc ttcagttgca gttcgtgagc gatttgttgc tcaacgatga cctcgtaacc
     3061 ttttgtgcac agccagcggt agagcatttc atgtgttgtc agtgcagtgg ggtgccgtgg
     3121 gtgtcccaca atgccaatac acttgaaatg attattcatt tttccgaggt ccttgttgcg
     3181 aagattgatg acaatgtgag tgcttccctt gaaaccctga aactgatccc cataataagc
     3241 gaagttagcg agatgaatgc gaaaaaaacg cggagaaatt catgagtagt aaagaacaga
     3301 aaacgcctga ggggcaagcc ccggaagaaa ttatcatgga tcagcacgaa gagattgagg
     3361 cagttgagcc agaagcttct gctgagcagg tggatccgcg cgatgaaaaa attgcgaatc
     3421 tcgaagctca gctggctgaa gcccagaccc gtgaacgtga cggcattttg cgtgtaaaag
     3481 ccgaaatgga aaacctgcgt cgacgtactg aactggatat tgaaaaagcc cacaaattcg
     3541 cgctggagaa attcatcaac gaattgctgc cggtgattga tagcctggat cgtgcgctgg
     3601 aagtggctga taaagctaac ccggatatgt ctgcgatggt tgaaggcatt gagctgacgc
     3661 tgaagtcgat gctggatgtt gtgcgtaagt ttggtgttga agtgatcgcc gaaactaacg
     3721 tgccgctgga cccgaatgtg catcaggcta tcgcaatggt ggaatctgat gacgttgcgc
     3781 caggtaacgt actgggcatt atgcagaagg gttatacgct gaatggtcgt acgattcgtg
     3841 cggcgatggt aactgtagcg aaagcaaaag cgtaatttct gttttcgtaa taattcacgg
     3901 ccctgcatgc gaatgcaggg ccgttttcgt tactccgcca cactctcgcg cagcggtttc
     3961 acaggaaaaa cttttacctg cttaatcata ttgtcctgta cgtcgagaat atctatatcg
     4021 tactcgccaa tacgcacgcg ggtgcctgcg acagggatct cttccagtgc ctcaagaatg
     4081 acgccattaa ccgtgcgggc atcatcttcc ggtagatgcc agttaaaggc tttgttgatt
     4141 tcccgcacgt tggcggtgcc atcgataatc accgaaccgt cgttttgcgg cgtgacctct
     4201 tcggcaagtg ttggcgacat cgacgtagtg aaatcgccga caatctcttc cagaatatct
     4261 tcaaccgtca ccagcccctg aatgtctcca tactcgttga cgaccaggcc gactttcttt
     4321 ttgttgcgct gaaactttac caactgcgtg ctgagcggcg taccttccgg cacaaaatag
     4381 atctcgtccg cggcgcgcag catggtttct ttggtgaact cttttttctc cgacatcaac
     4441 cgccaggctt cacgtactcg cagcatactg atggcgtcgt ccagcgaatc acggtagagc
     4501 acgatgcgcc cgtgaggtga gtgggagagt tggcgcagaa tcgatttcca gtcatcgttg
     4561 atatcaatac cgataatttc actgcgcggc accatgatgt catcaacggt cattttttcc
     4621 agatcgagca ccgacagcag catatcctga ttgcgacggg aaatttgtga gcgtgattcg
     4681 tgcacgatag tgcgcaactc ttctttgctc aaagagccgc taaccacgat atcggttttg
     4741 atacccatca tgcgcatcag catacgggtg atagcattca gcaaccagac cagcggcatc
     4801 atcaaaattt gcagcggagc cagcagaaaa ctactcggat aagcgacttt ttccgggtac
     4861 agcgcggcaa tggttttcgg caatacctca gcaaatacca gtacgacaaa agtcagcaca
     4921 ccagtcgcaa ttgccacgcc cgcatcgccg tacaaacgca tcccaacaat agtgccgagc
     4981 gcggaggcaa gaatattgac caggttattg ccgattaaca ccaggcttat caggcggtct
     5041 ggcttacgca gcaatttttc gacgcgtttg gccgagcgat taccctgttt cgccatatgt
     5101 cgcagacgat agcggttgag ggtcatcatt ccggtttcgg acccggaaaa ataggctgaa
     5161 atgaccacca tgatgatcag aataatgatc aacgtagtag tagaaatgtg ttccagggga
     5221 aactcctttt ctgggtttag ctgattaact gctggacaat tcggctgccg aagtaggcca
     5281 gtgtcagaat taccgcgccc gcaacgttaa accagacgac gcggcgtcca cgccatcctt
     5341 catggtaatg tccccacagc agcacaatat agacaaacca cgccacgata gagagcacag
     5401 ccttgtcgat attttccatg ctaaataggt tgtgcatgta gaacaggcca gtgcaaagcg
     5461 tgagcgttag cagcaccacg ccaatctgcg tgatgtggaa cattttacgc tcgatactca
     5521 tcaatggcgg catttcctgg ttaaatgcca gcttcttgtt cttcagttgg taatcaatcc
     5581 acgccagttg cagcgcgtac agggcggcga taattagcgt ggcataggaa aagagcgata
     5641 agccaatgtg caccagcatc ccaggcgttg cttccagatg ggtgatgtat tcattgggca
     5701 tgaaggtcgc cagcgccagg ttgataagcg caaaggcata gacaatgggt agcagcaacc
     5761 agccacgatt gcgagaagcc acaatggtca ttaccgtaca gatcatcaaa ctgaccaatg
     5821 aaccaacgtt cagcaggctg aggttttgtc cgctatcacc gtcgggcagg atgcgggctt
     5881 ccagagcgat tgcgtggcag accagcgcaa tgaccgcaga aataatagcc atgcgccgcc
     5941 agccgccgtt tttttgcagc agaccgggaa caatcagcgc aagactgacg gagtaggcga
     6001 caagcgcgag cagagcaaaa acgggcatag tgatgtcgac agtttgagca ataagaaaga
     6061 gaagcagtat agcgtcaggt gaacgctgct ccaaccgttg cataacaaca aagacgcctt
     6121 catgttatac tgcggcaaaa tactgatgat gtgtcgcgat tgcggccaac cgtttccacc
     6181 ccaggcgaga gacaatgttt gataatttaa ccgatcgttt gtcgcgcacg ctgcgcaata
     6241 tcagtggccg tggacgcctc actgaagaca acgtaaaaga tacgctgcgc gaagtgcgca
     6301 tggcgctgct ggaggcggat gtcgctctgc cggtagtgcg tgagtttatc aatcgcgtaa
     6361 aagagaaagc ggttggtcat gaagttaata agagcctgac gccggggcag gagttcgtca
     6421 aaatcgtccg taacgaactg gttgcggcga tgggcgaaga gaaccagacc ctgaacctgg
     6481 cggcgcaacc gcctgcggtc gtgctgatgg cgggcctgca aggtgccggt aaaacaacca
     6541 gcgttggtaa actcggtaag ttcctgcgcg agaagcacaa gaagaaagtg ctggtggttt
     6601 ctgccgacgt ttatcgcccg gcggcaatca aacagcttga gacgctggca gagcaggtgg
     6661 gcgttgattt cttcccttct gatgttggtc agaagccggt agatatcgtt aacgcggcgc
     6721 tgaaagaagc caaactgaaa ttctacgacg tgctgctggt ggataccgct ggtcgtctgc
     6781 acgttgacga agcgatgatg gacgagatca aacaagtcca tgcgtcgatt aacccggttg
     6841 aaaccctgtt tgtggttgac gccatgaccg gtcaggatgc ggccaatacg gcaaaagcat
     6901 tcaatgaagc gttaccgctt accggcgtag tgttgaccaa agtggacggc gatgcccgcg
     6961 gcggtgcggc gctctctatt cgtcacatca ctggcaaacc gatcaagttt ctcggtgttg
     7021 gcgagaaaac tgaggcgctg gagccgttcc atccggaccg catcgcgtcg cgtattctcg
     7081 gcatgggcga cgtactgtcg ctgatcgaag atatcgaaag caaagttgac cgcgcgcagg
     7141 cagagaaatt agccagcaag ctgaaaaaag gcgacggctt cgatctcaac gattttcttg
     7201 agcagctgcg ccagatgaaa aatatgggcg gcatggctag cctgatgggc aagctgccgg
     7261 gcatggggca gatcccggat aatgttaagt cgcagatgga cgataaagtg ctggtgcgta
     7321 tggaagccat catcaactcg atgacgatga aagagcgcgc taagccagaa atcatcaaag
     7381 gttcgcgtaa gcgtcgtatc gctgccggtt gcggtatgca ggtgcaggac gttaaccgcc
     7441 ttctgaagca gttcgacgac atgcagcgca tgatgaagaa aatgaagaag ggcggaatgg
     7501 cgaagatgat gagaagcatg aagggtatga tgcccccagg cttccctggt cgctaatcgg
     7561 cctggcttgt ttgccatttt ggcaccggga tgtgctcgtc atcctcacgt actgcgtgta
     7621 cgctccggtg actgcgcgca ctccggcacc aaactggctg caccgccgac ggccttttac
     7681 agcagagtta gcaactgttg attgcaattc ccgcaaaaat gagtaaaatt ttcgggcttt
     7741 taatatgaca ccggactccg ttcctcgatg gggtccggtt gttttattca cacaagagga
     7801 tgttatggta actattcgtt tagcacgtca cggcgctaaa aagcgtccgt tctaccaggt
     7861 tgttgtcgct gacagccgta atgcacgcaa cggtcgcttc atcgagcgcg ttggtttctt
     7921 caacccaatc gctagcgaaa aagaagaagg cactcgcctg gatctggatc gcatcgctca
     7981 ctgggttggc cagggcgcaa ctatttctga tcgcgttgct gcgctgatca aagaagtaaa
     8041 caaagcagct taatctgtca cggtggtcat gatgagcaaa caactcaccg cgcaagcacc
     8101 tgttgatccc atcgttttgg gaaaaatggg ttcgtcttac ggtattcgtg ggtggctcag
     8161 agtgttttct tccaccgaag acgccgaaag catttttgac tatcagccct ggtttatcca
     8221 gaaggcgggt cagtggcagc aagtccagct ggaaagctgg aagcaccaca atcaggacat
     8281 gatcatcaag ctgaaaggcg ttgacgatcg tgatgcggcg aacctgctga cgaattgtga
     8341 aattgtcgtg gattcatcgc agctgcctca gcttgaagag ggggactact actggaaaga
     8401 cctgatgggc tgccaggtag taaccactga aggctacgat ctcggtaaag tcgtcgatat
     8461 gatggaaacc ggatctaatg acgttctcgt cattaaggca aacctgaaag atgcgtttgg
     8521 tatcaaggaa cgtctcgtac cgttcctcga tgggcaggtt atcaagaaag tcgatctcac
     8581 tactcgttca atcgaagtag attgggatcc tggtttttaa accaccggat aaacggtaaa
     8641 agacggcgct atgtggattg gcataattag cctgtttcct gaaatgttcc gcgcaattac
     8701 cgattacggg gtaactggcc gggcagttaa aaatggcctg ctgagcatcc agagttggag
     8761 tcctcgcgac ttcacgcatg accggcaccg taccgtggac gatcgtcctt acggtggcgg
     8821 accggggatg ttaatgatgg tgcaaccctt gcgggacgcc attcatgcag caaaagccgc
     8881 ggcgggtgaa ggcgcaaagg tgatttatct gtcaccacag ggacgcaagc ttgatcaagc
     8941 gggcgtcagc gaactggcaa cgaatcaaaa attgattctg gtgtgcggtc gctacgaagg
     9001 tatagatgag cgcgtgatcc aaaccgaaat tgacgaagaa tggtcaatcg gcgattacgt
     9061 tctcagtggt ggtgagttac cagcaatgac gctgattgac tccgtttccc ggtttattcc
     9121 gggagtactg ggacatgaag cctcggcaac ggaagattcc tttgctgaag gattgctgga
     9181 ttgcccgcac tatacgcggc ctgaggtgtt agaagggatg gaagttccgc cagtgttact
     9241 gtcgggcaac catgccgaga tacgtcgctg gcgtttgaaa cagtcgctgg gccgtacctg
     9301 gcttagaaga cctgaacttc tggaaaacct ggctctgact gaagagcaag caaggttgct
     9361 ggcggagttc aaaacggaac acgcacaaca gcaacataaa catgatggga tggcgtaagc
     9421 ccccgagata tcagtttacc caggataaga gattaaatta tgagcaatat tattaagcaa
     9481 cttgaacaag agcagatgaa gcaggacgta ccttccttcc gtccgggtga taccgtggaa
     9541 gtgaaagtat gggttgttga aggttccaaa aaacgtctgc aggcattcga gggcgtggtt
     9601 atcgctattc gtaaccgcgg tctgcactct gcattcactg ttcgtaaaat ttccaacggc
     9661 gaaggcgttg agcgtgtctt ccagactcac tctccggtag ttgacagcat ttctgtcaaa
     9721 cgtcgtggtg ctgttcgtaa agctaaactg tactacctgc gtgagcgtac tggtaaggct
     9781 gctcgtatca aagagcgtct taactaagat ttcgcttaag cgacatcctg ttaagaaggg
     9841 ctggccaatt ggctggccct ttttttgtct gtttgctcgt gttttaaggg gttgtaatca
     9901 ccactgcgac gcggcggttc tcggcgcggc cctgggcggt cttgttactg gctatgggat
     9961 atttttttcc taaaccctgt gtggtgagat tgctgcgtgg aatttgtcca ccaatagccc
    10021 atgcatcggc cacgacattc gcccgtttca atgataagcc ttcgttgtaa ctgtcttcac
    10081 catagttatc ggtgtgtcca tccatacggg catgtgttag ccctgtcgag gccaatttgg
    10141 ctgccatggt ttggatctgt tgctggcttt ccgggagcaa tttgtagtca ttttttgcga
    10201 acagaatggc atctgataag cccagcgacc agtcgccggc ggattcagta aatccataag
    10261 attgcatagc ggcgacttgc tcaggagtaa actttccctg aggggactgg cagccagtca
    10321 gtattagtga ggtgaaaatc aggggtgcta ctaggtgctt tatcatatcg atatatcctt
    10381 gttatctcac cagcttttcg gcacgctggt gtttggcctg atacatattg tgatcggcaa
    10441 gctcttgtaa tttttccgca gaggcgtgct caatggtcat cgcgtaacca atgcttaatg
    10501 tcatggtggt ctgatggcca ttatgaagat caaacgggag attaaagatt tgtgtcagtg
    10561 ctgagcatat ctgctgcact tcagattctg actgtacatc atagagcacc atagcgaatt
    10621 catcgccgcc caggcggtat gctttatgtc gcagcccgcc aacttcagct aaccgttttg
    10681 cgatttcaat caagactcta tcgcccgtcg catgacccca ggtatcattg atgtatttga
    10741 aattatcgcc atcaagaaat agtaacgccg acgtttttcg ggcatcggaa ttgttcatca
    10801 acgtgttgat gccgctacga aacgctgcgc ggttagccag cccggttaat gggtcatgta
    10861 gcgcggtacg tagaagctgc gcatttttag cctgtaaacg aagctgccac tcttccattt
    10921 catccagcag actgttgaag tcgagagcga agcggtgaaa ctcagcgata cgttcttccg
    10981 aaactcgtcg ggaaaaattg cggttggaac gcacatcatg tacgacatcg gtgatatttt
    11041 tcagtgcttc caccaggcca ttgtgcaaat ggcgggtgag ggtaattgcg atgcctgatg
    11101 ccagcagaat acaaccggtc agtacggcga gcgaaaacca gataaaatgg ctgattgaac
    11161 tgtcgcgagc ggttaagcgt acttcgccaa tggtttcacc attgtgacga atcggctgaa
    11221 tgatgggggc ggggaagagc cagtggctta tgaaattgct gaaagtatcg cctggatcct
    11281 tacgggtgta atgccaggac gccagaatat tttgctgctt atcacgtact tctgcagttg
    11341 aaaattgccc ttgctggccc agcgctgcca gtgtttcagt tgctgcaggg ccatcggcaa
    11401 aaacgacagc tgcttccaga ctgtaagt
//
LOCUS       DAJVYM010000119        11355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVYM010000119 DAJVYM010000000
VERSION     DAJVYM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.120"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="OQS60_003010"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="OQS60_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCS2388706.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="OQS60_003011"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="OQS60_003011"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS2388707.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="OQS60_003012"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="OQS60_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS2388708.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="OQS60_003013"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="OQS60_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS2388709.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="OQS60_003014"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="OQS60_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS2388710.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="OQS60_003015"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="OQS60_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS2388711.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="OQS60_003016"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="OQS60_003016"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS2388712.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="OQS60_003017"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="OQS60_003017"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS2388713.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="OQS60_003018"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="OQS60_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS2388714.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="OQS60_003019"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="OQS60_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS2388715.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="OQS60_003020"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="OQS60_003020"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS2388716.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="OQS60_003021"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="OQS60_003021"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS2388717.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="OQS60_003022"
     CDS             10898..>11355
                     /locus_tag="OQS60_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS2388718.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2872 a         2631 c         3056 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAJVYM010000120        11306 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVYM010000120 DAJVYM010000000
VERSION     DAJVYM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.121"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..210
                     /locus_tag="OQS60_003023"
     CDS             <1..210
                     /locus_tag="OQS60_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2388719.1"
                     /translation="KIVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMF
                     AQTTKGVLETGIYRGVVSIYLSQDI"
     gene            302..2986
                     /locus_tag="OQS60_003024"
     CDS             302..2986
                     /locus_tag="OQS60_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS2388720.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2983..4071
                     /locus_tag="OQS60_003025"
     CDS             2983..4071
                     /locus_tag="OQS60_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS2388721.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="OQS60_003026"
                     /pseudo
     CDS             complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="OQS60_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5934..7346
                     /gene="uxaC"
                     /locus_tag="OQS60_003027"
     CDS             5934..7346
                     /gene="uxaC"
                     /locus_tag="OQS60_003027"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS2388722.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7361..8848
                     /gene="uxaA"
                     /locus_tag="OQS60_003028"
     CDS             7361..8848
                     /gene="uxaA"
                     /locus_tag="OQS60_003028"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS2388723.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8931..9482
                     /locus_tag="OQS60_003029"
     CDS             8931..9482
                     /locus_tag="OQS60_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS2388724.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="OQS60_003030"
     CDS             complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="OQS60_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS2388725.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2969 a         2773 c         2932 g         2632 t
ORIGIN      
        1 aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc
       61 acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa
      121 aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta ccgtggtgta
      181 gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca cgtaacaaag
      241 ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt tcaggattgc
      301 catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg cagaggcgac
      361 attaaccgaa attcccgcag ggtttgaggt tattgctcag ggacagcagg agtatatcga
      421 ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc ttgataccgt
      481 aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag acgatcagaa
      541 aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcaccacg gtgaattggc
      601 ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata cgctggaaat
      661 aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat ggaattcagc
      721 tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg cttttataca
      781 tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta ttcagggaaa
      841 cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt tcaacggtta
      901 tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt atcgttatga
      961 ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac tatttaatgc
     1021 acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg ggatgcgtat
     1081 cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt
     1141 tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt
     1201 ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag gcagctacag
     1261 cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac
     1321 caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac
     1381 atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca
     1441 tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt
     1501 aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca gcgccagcgt
     1561 gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg
     1621 atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag
     1681 cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg tttcacagaa
     1741 ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca ctgatgatag
     1801 tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga caacagcgca
     1861 aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc gtgactggat
     1921 tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa
     1981 cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag
     2041 ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg atcaaacgtc
     2101 atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac
     2161 cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca
     2221 atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct
     2281 ttcggccttt acgggcactt atagctctac gctggctgtc agttgctatg gcgttaattt
     2341 gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc
     2401 tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac
     2461 tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt
     2521 tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg
     2581 tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc
     2641 cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc
     2701 gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggaggat ttacacttga
     2761 aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc
     2821 actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt
     2881 ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa
     2941 attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt
     3001 gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc
     3061 agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa
     3121 gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa
     3181 acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt
     3241 gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa
     3301 gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga
     3361 tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc
     3421 taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt
     3481 aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc
     3541 gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag
     3601 ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg
     3661 ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc
     3721 aaagatgaag ggcgaccagc tgcagggcgc agagacggtg acttctctat ctatcgtcaa
     3781 ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg
     3841 gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac
     3901 ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg
     3961 acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac
     4021 atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaattg accccgcaga
     4081 ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc ttcgtcatta caacttacgt
     4141 taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     4201 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     4261 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     4321 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     4381 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     4441 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     4501 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     4561 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     4621 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     4681 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca
     4741 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4801 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4861 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4921 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4981 ttgccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     5041 gcctactgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     5101 cgggatcatc gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca
     5161 gctacctgcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     5221 accgattttc gtacccagca catccagtac ataacctgct accggttgca tgaccgtata
     5281 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     5341 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     5401 gagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     5461 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     5521 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     5581 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     5641 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     5701 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5761 aggccagtac tggcgcggcc tgcagcgaga tttatcactt tgagagtaat ttttttaact
     5821 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     5881 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     5941 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     6001 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     6061 aagactatcg ttttaaaaac ctgtatgaca tctggctgaa aggcgatcac tacaaatggc
     6121 gcgctatgcg taccaacggt gtggccgagc gtctgtgtac cggtgatgca tctgaccgtg
     6181 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     6241 ggacgcacct tgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa
     6301 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     6361 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     6421 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     6481 gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga
     6541 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     6601 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     6661 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     6721 ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     6781 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     6841 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     6901 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     6961 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     7021 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     7081 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     7141 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     7201 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     7261 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     7321 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     7381 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     7441 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     7501 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     7561 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     7621 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga
     7681 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct
     7741 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     7801 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc
     7861 acaactgggc gacgatcaca tcaacatccg caccatgctg caaaacatgg tgcgccaccc
     7921 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     7981 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     8041 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     8101 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     8161 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     8221 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     8281 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     8341 ctttaaacag tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa
     8401 caaagcgggc ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg
     8461 ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc ctgggctgaa
     8521 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     8581 ccatatggtg ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt
     8641 gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     8701 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     8761 cgttgagttt gccaacggta agcaaacttg caacgagcgt aacgacttcc gcgaactggc
     8821 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     8881 ttcataatgt gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt
     8941 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
     9001 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
     9061 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
     9121 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
     9181 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
     9241 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
     9301 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacaatga
     9361 ttgagggtag ttttctgctt atgaatggct tgaatatcat tcgtttctgg cggatgcaga
     9421 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccgtagac gaaaggggtt
     9481 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
     9541 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
     9601 gccgatgata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
     9661 attacaggcc agcgggatca gcagcagaga accccccgcc acgccggatg cgccacaggc
     9721 acacagagaa gccaccacgc tcaacagcag cgccgtgggc agatcgaccg ggatacccag
     9781 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat
     9841 ggtggcaccc agcggaatag aaacggaata ggtatcgcga tccagattca gcttttcaca
     9901 cagtgccata ttcaccggaa tgttcgccgc agagctgcgg gtgaagaagg catacacgcc
     9961 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
    10021 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
    10081 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagacc
    10141 aaaaatacca atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga
    10201 catatcgttg accaggtttt tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc
    10261 aattgcccac accaggatcc cgatgtagtt acctttcagc agcgcgtcga tggggttgga
    10321 aaccatgctc attaccagcc cgcgcatcac ttctacaatg cctgacggtg gcgaaatatc
    10381 acccgcgcta ctggataagt gcagggtaga agggaaggcg aagctgaaaa cgactgcggc
    10441 cagagcagca gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt
    10501 ctgcccatgc tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc
    10561 aacggctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    10621 ttttgatatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    10681 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    10741 ctttcattgt gttatcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    10801 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    10861 gattttaaag agaagtcata ttttgaaact taatggttac atttgtttga cataaacaga
    10921 gggaaggact ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg
    10981 agaacggttg cgctccttta atgtgctatg ccatataaag tcactggcgc agggagcgcg
    11041 cagggggcgg ccaatcgccg ccgccccctg cacccccagg ctctggcgaa caaaatcgcc
    11101 gctgcgcgat tccctcagct tatcccttgc ggctaccggg tcgggcgcaa gggaacgtcc
    11161 ctgtaagatg cgccctcgcc gcgcatccat gcgcgtcgcc ccggcctcca gggaacgctt
    11221 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta caagccgatt
    11281 acataattaa tagctcacct ttgtct
//
LOCUS       DAJVYM010000121        11231 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVYM010000121 DAJVYM010000000
VERSION     DAJVYM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.122"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..308
                     /locus_tag="OQS60_003031"
     CDS             <1..308
                     /locus_tag="OQS60_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS2388726.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="OQS60_003032"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="OQS60_003032"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS2388727.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OQS60_003033"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OQS60_003033"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS2388728.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="OQS60_003034"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="OQS60_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS2388729.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="OQS60_003035"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="OQS60_003035"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS2388730.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="OQS60_003036"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="OQS60_003036"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS2388731.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="OQS60_003037"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="OQS60_003037"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS2388732.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="OQS60_003038"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="OQS60_003038"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS2388733.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /locus_tag="OQS60_003039"
     CDS             complement(8116..9762)
                     /locus_tag="OQS60_003039"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS2388734.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /locus_tag="OQS60_003040"
     CDS             complement(9765..9815)
                     /locus_tag="OQS60_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388735.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="OQS60_003041"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="OQS60_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS2388736.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11231
                     /locus_tag="OQS60_003042"
                     /pseudo
     CDS             10353..11231
                     /locus_tag="OQS60_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2551 a         3087 c         2946 g         2647 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg c
//
LOCUS       DAJVYM010000122        11150 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVYM010000122 DAJVYM010000000
VERSION     DAJVYM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.123"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            459..962
                     /locus_tag="OQS60_003043"
     CDS             459..962
                     /locus_tag="OQS60_003043"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2388737.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1335..2528)
                     /locus_tag="OQS60_003044"
     CDS             complement(1335..2528)
                     /locus_tag="OQS60_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2388738.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2664..4388
                     /gene="iucA"
                     /locus_tag="OQS60_003045"
     CDS             2664..4388
                     /gene="iucA"
                     /locus_tag="OQS60_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS2388739.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4389..5336
                     /gene="iucB"
                     /locus_tag="OQS60_003046"
     CDS             4389..5336
                     /gene="iucB"
                     /locus_tag="OQS60_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS2388740.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5336..7078
                     /gene="iucC"
                     /locus_tag="OQS60_003047"
     CDS             5336..7078
                     /gene="iucC"
                     /locus_tag="OQS60_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS2388741.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7075..8412
                     /gene="iucD"
                     /locus_tag="OQS60_003048"
     CDS             7075..8412
                     /gene="iucD"
                     /locus_tag="OQS60_003048"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS2388742.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8418..10613
                     /gene="iutA"
                     /locus_tag="OQS60_003049"
     CDS             8418..10613
                     /gene="iutA"
                     /locus_tag="OQS60_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS2388743.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2786 a         2818 c         2885 g         2661 t
ORIGIN      
        1 tcaacgaagg ggccgacaaa cacgttgtcc ctgagttcac attcataaat gttgaccggt
       61 ttgacaatac ggacattgat cccataaatc acatcccgga cagcacattc cagaaatttc
      121 atactcacac gtcctttaaa tacagatgac atttgccgga attataccgc ccagattcac
      181 ttgccatgtt tcagaatagc ttataagccg cacttttacc gatactcagc aacaaatcag
      241 gtctgtatcc acttcagccg gaaaccggat ctgcgcggcg gggaacgggc aagaaatatg
      301 ataattaagc aacacacata aaatcatatt gcataatcgc atctgaatcg aacacttatt
      361 cgcatcacta tcgatcatca cttcgcatct cataccctta cccacaagtt gaaataatag
      421 tattatattg aaaagacaga gattatttca gaggtgttat ggaaaactgg gttattcagg
      481 agctaaaatc actggatata ggggactcaa gacttgataa aagagtaaaa catatcctca
      541 atagcctcag tcgttcacca gaagaaagta ttcctgtttc ctgccgtacc tggagtgaaa
      601 caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa attatggctc
      661 ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta gttttacagg
      721 atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt cacaaaaaac
      781 ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag tcaggtgttt
      841 gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc agtaaaaata
      901 cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa aaagaaacct
      961 agcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc cctgataccc
     1021 atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa cgcgcagagc
     1081 agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac agagcaacgc
     1141 tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg gagattacac
     1201 cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc agaacactgc
     1261 ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac tccagtaagg
     1321 tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga cacaatggta
     1381 aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc tgttttcgat
     1441 accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt agcgagccag
     1501 cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc gggtgttgcc
     1561 agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag ctgttcggag
     1621 agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag ggccaccgga
     1681 gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac tgcggacaga
     1741 cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtgacgaa ctggcgagta
     1801 agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa
     1861 agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg tgtgggctgt
     1921 ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggtgc tgccatcagt
     1981 agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag cggcccgaat
     2041 agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg attcccttct
     2101 ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac ggtcagcccg
     2161 taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat ggcgctggta
     2221 gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc tcccagagcc
     2281 cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca cggggacgat
     2341 ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag actcagattt
     2401 gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat accgagaagc
     2461 ccggcactga acgcaagtgg ccagcaggac agtgtttttt ggggcgtatc ttcgatccct
     2521 ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa aattaattta
     2581 tctgacaata acatttattg ttgataatga gaatcattat cgacataatt gttattattt
     2641 tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc acagatgtgg
     2701 ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa ctgacagaat
     2761 acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat ttcagagtgg
     2821 cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg gtcacggcat
     2881 caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat aagctacgcc
     2941 atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc
     3001 atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg cgtgaaaaag
     3061 cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc caccctgcgc
     3121 ctaagtctca tgaaccgttt aaccgacggg aggctgaacg ctacctgcct gatatggcac
     3181 cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc
     3241 atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct cagttactca
     3301 acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg gaatatcttt
     3361 tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac ttaggtgagg
     3421 ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc accagccgcg
     3481 atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact ctgtccgtga
     3541 aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc tggcagatgc
     3601 tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc gggctgctcg
     3661 atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg
     3721 atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca cctgatggcg
     3781 gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc
     3841 aacaggccgc ccatgcatgg gtcgatgcat actgtcagca agtgctaaag ccgctgttta
     3901 cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga
     3961 tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc gcttttatgc
     4021 ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat attttcaccc
     4081 atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact tttgctgtga
     4141 ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct cgtgtacgag
     4201 catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg
     4261 aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat cataacgaga
     4321 acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg caggctcagg
     4381 aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc tgtgaaaaac
     4441 tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg cactgtcccg
     4501 gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc gccgcaccac
     4561 aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag gcgctgacgc
     4621 tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag ttaccgttat
     4681 ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga
     4741 tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt tacgatccac
     4801 gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca gaacgtttca
     4861 cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc tcactggagg
     4921 tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg ctgattgggt
     4981 gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa gaccgcattg
     5041 gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt ggtgaacagc
     5101 aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc
     5161 tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac cagcgcctgt
     5221 tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc ccacataagc
     5281 gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa
     5341 tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga
     5401 gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca ttaacctgcc
     5461 gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt ggattgatgc
     5521 ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga tgcagctaaa
     5581 gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt tgtatgccac
     5641 gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat
     5701 taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat tcgtttttaa
     5761 taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag agtataccaa
     5821 caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc gctgcgataa
     5881 cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt ttacacgctt
     5941 cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctaccgctgc cggtacatcc
     6001 gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgtcg aaggcaggat
     6061 ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac
     6121 caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct acaacacctc
     6181 atgctaccgg ggaatccctg gcagatatat cgctgccgga ccactggctt cacgctggct
     6241 gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga tccttggtga
     6301 accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg ctccctatcg
     6361 ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc tgaaaccgga
     6421 tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc agccgctggc
     6481 aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc aattgttccg
     6541 ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca
     6601 cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga
     6661 cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact ctttgcctca
     6721 ggaggtgcgt gatgccacat cccgtctgag tgcggactac ttaatccatg atttgcagac
     6781 gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc ttggcgtacc
     6841 tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga acaaacatcc
     6901 acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca ttcgcgtggt
     6961 gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca tgctgccgaa
     7021 ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg aatcatgaaa
     7081 aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat tgctgcattg
     7141 tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca tttttcctgg
     7201 catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa agatctggtc
     7261 agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa gcacaaaaag
     7321 ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt ttctgactat
     7381 ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt tgaaaatatt
     7441 gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga atattttgcc
     7501 cgcaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt gaagcatgtg
     7561 acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa
     7621 cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa tgcattacgc
     7681 ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa ttttaacgca
     7741 ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc aggcttctcc
     7801 ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac atcggatggc
     7861 atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt tgaagttctg
     7921 agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaaccct ggaaagtagt
     7981 ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga gagcctggaa
     8041 agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat acttccctca
     8101 ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga tgacttcact
     8161 ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag catgcaaact
     8221 catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg tattcttaat
     8281 cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat tcagtggcgc
     8341 agcggcagcc ggaaaaaacc gcagccggag gctgcttctt taactcacta tacagcaaat
     8401 attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc
     8461 ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc atagtgtctg
     8521 ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg
     8581 ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg gctcagttaa
     8641 tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac atgcgtggcc
     8701 gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc gacagccgac
     8761 aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc ggcgcgacgg
     8821 ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa aaaggtcagc
     8881 cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc agtaaagatc
     8941 acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc ggacgtcttt
     9001 ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc accctgcttg
     9061 ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga accggtacgc
     9121 tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa agtcagggag
     9181 acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg agcagcacac
     9241 catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg catttgatca
     9301 gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag gtttactacc
     9361 gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa caggcgacag
     9421 ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact ctgaacagcc
     9481 aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag cgctttacct
     9541 ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg aacaaccata
     9601 agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg gccttcctgc
     9661 aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc tatcagtata
     9721 ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt gctgccggga
     9781 aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac tttctgttca
     9841 atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat ttttcccagg
     9901 gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt gcagcagtga
     9961 acggccatct ttccctgaca aagagcgtga acgtcagcga cagtaagctg gaaggcgtga
    10021 aagtcgattc ttatgaactg ggctggcgct ttaccggtga caacctgcgg actcaaatcg
    10081 cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg accatcagtg
    10141 tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac ctgatcccgg
    10201 atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg
    10261 gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca gcttacatta
    10321 actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc gacgtaagcg
    10381 atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt agttggcagc
    10441 ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac tataccactg
    10501 tctggggaca gcgtgcacct ctgtactact gcccgggtta cggccctgct tcactgtacg
    10561 actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc tgacggggat
    10621 gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg ttgtttacag
    10681 ccagtataat atacagcctt aacaagatat tccgtctccg gaatggactg ttcaccactg
    10741 aataacatta tttctgtttt atggatcttt gactgaaatt tatcgcatat cctcatttca
    10801 ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc tggcagatcc
    10861 tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt gctgcaatga
    10921 aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta aaaagaggta
    10981 cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc tgtcataaag
    11041 tccggtaatg gagattgtta atacgatggt gcacgatgca ttgtcggtgc ccggcccgga
    11101 ggatgccccg taactacata cggatcaggg ttggcaatat cgaatggcag
//
LOCUS       DAJVYM010000123        11016 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVYM010000123 DAJVYM010000000
VERSION     DAJVYM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.124"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(111..320)
                     /locus_tag="OQS60_003050"
                     /pseudo
     CDS             complement(111..320)
                     /locus_tag="OQS60_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(522..1166)
                     /gene="cheZ"
                     /locus_tag="OQS60_003051"
     CDS             complement(522..1166)
                     /gene="cheZ"
                     /locus_tag="OQS60_003051"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS2388744.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1177..1566)
                     /gene="cheY"
                     /locus_tag="OQS60_003052"
     CDS             complement(1177..1566)
                     /gene="cheY"
                     /locus_tag="OQS60_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS2388745.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1581..2630)
                     /gene="cheB"
                     /locus_tag="OQS60_003053"
     CDS             complement(1581..2630)
                     /gene="cheB"
                     /locus_tag="OQS60_003053"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS2388746.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2633..3493)
                     /gene="cheR"
                     /locus_tag="OQS60_003054"
     CDS             complement(2633..3493)
                     /gene="cheR"
                     /locus_tag="OQS60_003054"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS2388747.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3512..5113)
                     /gene="tap"
                     /locus_tag="OQS60_003055"
     CDS             complement(3512..5113)
                     /gene="tap"
                     /locus_tag="OQS60_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS2388748.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5159..6820)
                     /gene="tar"
                     /locus_tag="OQS60_003056"
     CDS             complement(5159..6820)
                     /gene="tar"
                     /locus_tag="OQS60_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS2388749.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6965..7468)
                     /gene="cheW"
                     /locus_tag="OQS60_003057"
     CDS             complement(6965..7468)
                     /gene="cheW"
                     /locus_tag="OQS60_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS2388750.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7489..9453)
                     /gene="cheA"
                     /locus_tag="OQS60_003058"
     CDS             complement(7489..9453)
                     /gene="cheA"
                     /locus_tag="OQS60_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS2388751.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9458..10384)
                     /gene="motB"
                     /locus_tag="OQS60_003059"
     CDS             complement(9458..10384)
                     /gene="motB"
                     /locus_tag="OQS60_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS2388752.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10381..>11016)
                     /locus_tag="OQS60_003060"
     CDS             complement(10381..>11016)
                     /locus_tag="OQS60_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS2388753.1"
                     /translation="LMAKSRQMEMFSLERDIENPRESEIFASYPRILADSVMLDFIVD
                     YLRLIISGHMNTFEIEALMDEEIETHESEAEVPANSLALVGDSLPAFGIVAAVMGGVH
                     ALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLASVLRQKSAETSKMMQCVKVT
                     LLSNLNGYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA"
BASE COUNT         2481 a         3117 c         2700 g         2718 t
ORIGIN      
        1 attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag ctgcaaccta
       61 cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata aatactgtga
      121 tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca
      181 aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc acgggaacgc
      241 gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc ttctgttttg
      301 tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt aaatgatgcc
      361 agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt tagggctttt
      421 gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac
      481 cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc aagactatcc
      541 aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt atcgacctga
      601 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
      661 tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat catccgctta
      721 atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
      781 ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc tgttaccagt
      841 tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
      901 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
      961 gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg
     1021 gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg caaactgtcg
     1081 cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
     1141 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
     1201 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc
     1261 ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac
     1321 tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca ggccatccat
     1381 attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
     1441 agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt
     1501 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
     1561 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccaac
     1621 atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc
     1681 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc
     1741 gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt caggatcacc
     1801 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc
     1861 cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc gccactacgc
     1921 gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct
     1981 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
     2041 cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac
     2101 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
     2161 ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa tggcttatgt
     2221 gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc
     2281 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc
     2341 agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc
     2401 attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca
     2461 acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga
     2521 tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
     2581 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
     2641 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt
     2701 tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
     2761 aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag aagatcgcat
     2821 caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca aaatcaacat
     2881 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
     2941 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc
     3001 ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg
     3061 gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg
     3121 aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga tccgcgagca
     3181 gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
     3241 acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat
     3301 ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
     3361 ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
     3421 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
     3481 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
     3541 gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg
     3601 aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct tctaccagcg
     3661 aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc
     3721 cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc acgcgggtca
     3781 ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc gaaccctgct
     3841 ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac
     3901 ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc tgctctccgg
     3961 cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaattccgt
     4021 cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt
     4081 gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca
     4141 gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca
     4201 tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt gagagatcgt
     4261 tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc ttacgcacat
     4321 cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa atggcggtga
     4381 tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca atgctgtcaa
     4441 aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac
     4501 tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc gaaatctgat
     4561 agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc agctgccagg
     4621 cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg aggaaatccg
     4681 aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa
     4741 aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca
     4801 taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg
     4861 ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg ttcagcgcgg
     4921 tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc tgattgctct
     4981 gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac
     5041 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
     5101 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta aatactcgtc
     5161 aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt gccggtggtt
     5221 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
     5281 ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca gcggcggcgg
     5341 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
     5401 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca atctcgccca
     5461 taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
     5521 cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg gctttgatct
     5581 cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc accacggcaa
     5641 aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat
     5701 tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc ttcgaactat
     5761 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
     5821 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
     5881 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
     5941 cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
     6001 catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
     6061 cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta ttcgctaggt
     6121 taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta agcaacatac
     6181 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
     6241 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
     6301 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
     6361 ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc agttcagtta
     6421 acgctgtgta atagttttta tatttttcat caatattacg actggtagcg accatttcag
     6481 gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc aatgttttcc
     6541 tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa tccatcatca
     6601 tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc caggttgacg
     6661 tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc tggctatggt
     6721 gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat acccccagca
     6781 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
     6841 agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca cccgccgcga
     6901 aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga ggaaatcggg
     6961 agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg ctgttcagca
     7021 gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt ccagtgagat
     7081 attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc gccgtcaatg
     7141 aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg agattcagga
     7201 cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta attcgtaagt
     7261 caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt
     7321 tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg atatcaatac
     7381 cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc tcgctggcca
     7441 gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc aggcggcggt
     7501 gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc
     7561 cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc gatagttact
     7621 ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca gcaaggcgta
     7681 gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg ttttcgcgcc
     7741 cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc gcacttccag
     7801 tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca gtgattccat
     7861 aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca tgccgtcgag
     7921 gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct gcttcgactg
     7981 gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca tgccgacgcc
     8041 gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa atatcagcat
     8101 cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc
     8161 cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc
     8221 gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg cggcgagccg
     8281 tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg tcagcgggtc
     8341 gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca gcgtcagttc
     8401 tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc gactaaaaac
     8461 atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac gggcgttacg
     8521 ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca gttcgctgga
     8581 acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa ttaattgatc
     8641 aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg ttttttcccg
     8701 ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg gggtggatat
     8761 ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca caaaacagag
     8821 taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga gcgaatccgc
     8881 ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca gcaggtcgac
     8941 ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc gactctgctc
     9001 atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg gcgtttcgcc
     9061 tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc tggcggcatc
     9121 cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa
     9181 aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt catcgagcag
     9241 gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc
     9301 tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg gcgcttccgg
     9361 ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt catcaaaaaa
     9421 tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg gctgatggca
     9481 ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt actggctcat
     9541 tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca
     9601 ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg
     9661 ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc agttcgcggc
     9721 gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc tcaccgctgg
     9781 cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata ccgttcagta
     9841 caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca ctgccggttc
     9901 taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc tggaccagat
     9961 cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc aactggtcga
    10021 gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga tcttcgatgt
    10081 tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg ccgggaattg
    10141 ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt ggagtccgga
    10201 agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc cacatcacca
    10261 gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac gatccatgtg
    10321 ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
    10381 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
    10441 cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
    10501 gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc actgcatcat
    10561 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa taaatccgta
    10621 agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg cacccagctc
    10681 ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag ccgcgacaat
    10741 accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga cttctgcctc
    10801 gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg tgttcatatg
    10861 accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc tatccgcgag
    10921 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cacgttccag
    10981 cgaaaacatc tccatctgcc gcgatttcgc catcaa
//
LOCUS       DAJVYM010000124        11000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVYM010000124 DAJVYM010000000
VERSION     DAJVYM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.125"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..277)
                     /locus_tag="OQS60_003061"
     CDS             complement(<1..277)
                     /locus_tag="OQS60_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388754.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="OQS60_003062"
     CDS             complement(444..1184)
                     /locus_tag="OQS60_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS2388755.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OQS60_003063"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OQS60_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS2388756.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OQS60_003064"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OQS60_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS2388757.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OQS60_003065"
     CDS             complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OQS60_003065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS2388758.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OQS60_003066"
     CDS             complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OQS60_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS2388759.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OQS60_003067"
     CDS             complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OQS60_003067"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS2388760.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7167..7598
                     /locus_tag="OQS60_003068"
     CDS             7167..7598
                     /locus_tag="OQS60_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2388761.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7621..8199
                     /locus_tag="OQS60_003069"
     CDS             7621..8199
                     /locus_tag="OQS60_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS2388762.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8200..8907
                     /locus_tag="OQS60_003070"
     CDS             8200..8907
                     /locus_tag="OQS60_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS2388763.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8895..9572
                     /locus_tag="OQS60_003071"
     CDS             8895..9572
                     /locus_tag="OQS60_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS2388764.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9566..10243
                     /locus_tag="OQS60_003072"
     CDS             9566..10243
                     /locus_tag="OQS60_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS2388765.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10215..10928)
                     /locus_tag="OQS60_003073"
     CDS             complement(10215..10928)
                     /locus_tag="OQS60_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS2388766.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10925..>11000)
                     /locus_tag="OQS60_003074"
     CDS             complement(10925..>11000)
                     /locus_tag="OQS60_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2388767.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2919 c         2740 g         2714 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3061 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3121 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3181 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3241 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3301 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3361 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat
     3421 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3481 tatcgatgtt catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3541 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg
     3601 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3661 aaacaccgtg tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca
     3721 gttcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3781 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3841 tgccgatttt ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3901 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt
     3961 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     4021 cagtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4081 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4141 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4201 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4261 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4321 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4381 catcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4441 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4501 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4561 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4621 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4681 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4741 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4801 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4861 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4921 gccgatgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4981 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     5041 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5101 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5161 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5221 gctgccaaca gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa
     5281 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5341 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5401 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5461 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5521 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt
     5581 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5641 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5701 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5761 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5821 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5881 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5941 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     6001 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6061 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6121 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6181 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6241 catggcggag tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa
     6301 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6361 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6421 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6481 ggagccatat acgccggtgc agtcgaggac tacatcaaca ccaagttcac gccaggggag
     6541 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6601 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6661 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6721 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6781 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6841 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6901 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6961 aatgattgtc cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc
     7021 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7081 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7141 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg
     7201 cacgctttta tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7261 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7321 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag
     7381 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7441 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7501 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7561 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7621 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7681 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7741 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7801 accaatttac tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg
     7861 atggtcatcg gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca
     7921 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7981 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     8041 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg
     8101 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8161 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8221 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg
     8281 atctatagcg cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcgggca
     8341 aaatatgtcg cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8401 tggctaacag aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt
     8461 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8521 cccgtgcaat gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8581 ctgtttgccg gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8641 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8701 atgcccgcgt taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8761 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8821 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa
     8881 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8941 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     9001 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9061 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9121 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9181 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9241 aaacagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9301 gccatcgtca cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg
     9361 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc
     9421 aggccagcga aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga
     9481 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9541 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc
     9601 tagcgcagca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9661 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9721 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9781 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9841 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9901 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9961 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
    10021 actctctccg ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10081 gagcgtgttt gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat
    10141 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10201 tgtaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10261 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10321 taaaaggctt ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc
    10381 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10441 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10501 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10561 caatcccctt ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct
    10621 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10681 ccatccatcg ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc
    10741 cagaaggtgg ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10801 cgccgctgcg gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg
    10861 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10921 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10981 ccattccgct aaaacgcgcc
//
LOCUS       DAJVYM010000125        10990 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVYM010000125 DAJVYM010000000
VERSION     DAJVYM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.126"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1224
                     /gene="narU"
                     /locus_tag="OQS60_003075"
     CDS             <1..1224
                     /gene="narU"
                     /locus_tag="OQS60_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS2388768.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1306..5046
                     /gene="narZ"
                     /locus_tag="OQS60_003076"
     CDS             1306..5046
                     /gene="narZ"
                     /locus_tag="OQS60_003076"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCS2388769.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5043..6587
                     /gene="narH"
                     /locus_tag="OQS60_003077"
     CDS             5043..6587
                     /gene="narH"
                     /locus_tag="OQS60_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS2388770.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6587..7282
                     /gene="narW"
                     /locus_tag="OQS60_003078"
     CDS             6587..7282
                     /gene="narW"
                     /locus_tag="OQS60_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS2388771.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7279..7959
                     /gene="narI"
                     /locus_tag="OQS60_003079"
     CDS             7279..7959
                     /gene="narI"
                     /locus_tag="OQS60_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS2388772.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8038..8931
                     /locus_tag="OQS60_003080"
     CDS             8038..8931
                     /locus_tag="OQS60_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCS2388773.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="OQS60_003081"
     CDS             complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="OQS60_003081"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCS2388774.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10045..10614
                     /locus_tag="OQS60_003082"
     CDS             10045..10614
                     /locus_tag="OQS60_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCS2388775.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="OQS60_003083"
     CDS             complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="OQS60_003083"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCS2388776.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
BASE COUNT         2555 a         2866 c         2956 g         2613 t
ORIGIN      
        1 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
       61 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      121 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      181 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      241 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      301 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      361 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      421 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      481 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      541 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      601 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      661 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      721 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      781 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      841 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      901 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
      961 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1021 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1081 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1141 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1201 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1261 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1321 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1381 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1441 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1501 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1561 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1621 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1681 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1741 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1801 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1861 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1921 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     1981 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2041 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2101 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2161 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2221 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2281 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2341 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2401 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2461 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2521 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2581 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2641 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2701 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2761 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2821 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2881 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     2941 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3001 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3061 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3121 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3181 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3241 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3301 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3361 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3421 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3481 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3541 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3601 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3661 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3721 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3781 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3841 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3901 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     3961 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4021 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4081 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4141 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4201 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4261 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4321 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4381 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4441 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4501 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4561 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4621 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4681 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4741 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4801 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4861 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4921 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     4981 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5041 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5101 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5161 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5221 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5281 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5341 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5401 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5461 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5521 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5581 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5641 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5701 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5761 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5821 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5881 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     5941 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6001 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6061 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6121 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6181 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6241 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6301 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6361 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6421 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6481 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6541 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6601 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6661 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6721 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6781 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6841 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6901 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     6961 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7021 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7081 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7141 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7201 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7261 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7321 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7381 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7441 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7501 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7561 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7621 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7681 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7741 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7801 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7861 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7921 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     7981 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8041 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8101 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8161 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8221 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8281 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8341 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8401 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8461 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8521 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8581 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8641 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8701 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8761 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8821 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8881 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     8941 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9001 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9061 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9121 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9181 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9241 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9301 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9361 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9421 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9481 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9541 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9601 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9661 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9721 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9781 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9841 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9901 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
     9961 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10021 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10081 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10141 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10201 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10261 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10321 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10381 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10441 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10501 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10561 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10621 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10681 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10741 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10801 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10861 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10921 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    10981 gtagtttctg
//
LOCUS       DAJVYM010000126        10929 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVYM010000126 DAJVYM010000000
VERSION     DAJVYM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.127"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..312)
                     /locus_tag="OQS60_003084"
     CDS             complement(<1..312)
                     /locus_tag="OQS60_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCS2388777.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQS60_003085"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQS60_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS2388778.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OQS60_003086"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OQS60_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS2388779.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OQS60_003087"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OQS60_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS2388780.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OQS60_003088"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OQS60_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS2388781.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OQS60_003089"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OQS60_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS2388782.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OQS60_003090"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OQS60_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS2388783.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVGFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OQS60_003091"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OQS60_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS2388784.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="OQS60_003092"
     CDS             complement(8292..9365)
                     /locus_tag="OQS60_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2388785.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10929)
                     /locus_tag="OQS60_003093"
     CDS             complement(9376..>10929)
                     /locus_tag="OQS60_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2388786.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2831 c         2479 g         2768 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac ccaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttac
//
LOCUS       DAJVYM010000127        10897 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVYM010000127 DAJVYM010000000
VERSION     DAJVYM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.128"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            121..462
                     /locus_tag="OQS60_003094"
     CDS             121..462
                     /locus_tag="OQS60_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS2388787.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(453..1379)
                     /locus_tag="OQS60_003095"
     CDS             complement(453..1379)
                     /locus_tag="OQS60_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388788.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1469..2467
                     /locus_tag="OQS60_003096"
     CDS             1469..2467
                     /locus_tag="OQS60_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS2388789.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2464..2682)
                     /locus_tag="OQS60_003097"
     CDS             complement(2464..2682)
                     /locus_tag="OQS60_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS2388790.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2684..4699)
                     /gene="ligA"
                     /locus_tag="OQS60_003098"
     CDS             complement(2684..4699)
                     /gene="ligA"
                     /locus_tag="OQS60_003098"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS2388791.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4770..5756)
                     /gene="zipA"
                     /locus_tag="OQS60_003099"
     CDS             complement(4770..5756)
                     /gene="zipA"
                     /locus_tag="OQS60_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS2388792.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5986..6747
                     /gene="cysZ"
                     /locus_tag="OQS60_003100"
     CDS             5986..6747
                     /gene="cysZ"
                     /locus_tag="OQS60_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS2388793.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6932..7903
                     /gene="cysK"
                     /locus_tag="OQS60_003101"
     CDS             6932..7903
                     /gene="cysK"
                     /locus_tag="OQS60_003101"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS2388794.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8287..8544
                     /gene="ptsH"
                     /locus_tag="OQS60_003102"
     CDS             8287..8544
                     /gene="ptsH"
                     /locus_tag="OQS60_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS2388795.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8589..10316
                     /gene="ptsI"
                     /locus_tag="OQS60_003103"
     CDS             8589..10316
                     /gene="ptsI"
                     /locus_tag="OQS60_003103"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS2388796.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10357..10866
                     /gene="crr"
                     /locus_tag="OQS60_003104"
     CDS             10357..10866
                     /gene="crr"
                     /locus_tag="OQS60_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS2388797.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2652 a         2807 c         2702 g         2736 t
ORIGIN      
        1 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt
       61 tcttctcaag ttccttgttc ttctgccgat attacctcgt acagtgacga ggactgaaca
      121 atggccacta tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca
      181 tcacaaggta gcgatgttgc ttcaaaaatc gcgcggataa gtcagcaaat catcaaactc
      241 actcaacaaa ttaaagagat tgtagacact agcggcagtg ctgaagacaa acaaaaacag
      301 gccgaattga ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaga
      361 cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta
      421 aatcctgttg aaaatacgac tcatatcgat atctatatct gatacaacgg agccccttcc
      481 cgaccagcca ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg
      541 gtggaaagtg aacggttctt cctgcgcacc agcatcaact ggcgttcaac aaccggcgta
      601 atgcgcttta ccaccagtgg gctaccttca ggcaatggta gcgccagtgc gggaagaata
      661 ctgatgccga tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc
      721 acaatattcg cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct
      781 gacgcgtaat cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct
      841 tccacggcca aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcgct
      901 tcacattgca gatcgcccac tgggcctgga tcgatgacga tgcaaaaatc gacatctccc
      961 tggcgaatac tttccatcac ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct
     1021 ggatagcggc gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg
     1081 ctggcagcga cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac
     1141 gtgctgttaa gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct
     1201 gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc
     1261 tccttcacac tgtgacttac tgccgactgg ctcaagccga tacgctctcc tgcacgacta
     1321 aaacttttct cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc
     1381 tgttttcttc atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca
     1441 atagaaccat cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac
     1501 cttaaccctg atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt
     1561 ccccttcttt gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc
     1621 gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt
     1681 aatgtgcagc accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc
     1741 ggtaaatgtc gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac
     1801 cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg
     1861 ttctgcgtca gcatccagcc tgctggggat tttcctttca ccattgctag ttggtctggt
     1921 gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact
     1981 gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg
     2041 caataaaaaa tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc
     2101 gttcagcgaa gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt
     2161 tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc
     2221 acgccgactg ggcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa
     2281 gagtctggca aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat
     2341 gatggtgctg cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc
     2401 gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa
     2461 agcttaaagc ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag
     2521 cagcgtgatt tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa
     2581 ccacagcaga tactcctccg gcaggtcgat taagcgacgc cctttgtatt tgccaaacgg
     2641 cattatcgta ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac
     2701 gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag
     2761 atcctgcagc ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt
     2821 tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc
     2881 ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga
     2941 tcggcgcagg ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt
     3001 cttcggcaaa gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt
     3061 gcagctcttc aatcgaagcg gcttccagca cttccagcgt gccgaaatat gccgccagac
     3121 ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg
     3181 tggtttcttt cgctttttcc agcgcgttaa ccacgttttg tgccgatttt ggccccatac
     3241 gctccagtcc ggtcagtttg cctgcggtga gtttgaacag atctgccgga gtgtggacat
     3301 attctttttc aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac
     3361 ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg
     3421 tacagcgggc aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat
     3481 gcgtcgggaa tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca
     3541 cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa
     3601 gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact caccagcacg ccagcaacat
     3661 ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga
     3721 cgtcacgcac aaaggtcatc tgctcctgcg ccgggaattt aaacgctacc gcccagcgcg
     3781 gggcacgcgc gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca
     3841 cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca
     3901 gcacttcttc cgccgattca caaagcgtta cccgatcgct gaccggcaac ccccactttt
     3961 taaattgcag taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa
     4021 cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca
     4081 gtgaaccagc tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat
     4141 cttcgttaat cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac
     4201 gcgccgggat attctctccg tgcagcttca gcggaatggc gcgaatagta cgcacattag
     4261 acgtgatatc ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat
     4321 tttcatacag aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt
     4381 tctcgttgtt tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat
     4441 caaaaacgtt atccagtgac agcattggta cttcatggcg tatctggctg aaagccgcca
     4501 gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg
     4561 tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg
     4621 catccatcac atgataaaga tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt
     4681 gttgttcgat tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt
     4741 cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg
     4801 atgtcctggt actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg
     4861 acaccgccca cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc
     4921 agctcgtcac cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc
     4981 ttcatttcag gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg
     5041 ccgctgccat ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc
     5101 gcttgttgaa tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg
     5161 acgttcatga taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc
     5221 gctacaggct caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc
     5281 ggttgcggtg ctgaatgcac aggctgcggc gctggcgcga cctgtggcga aactggctgc
     5341 tgcaacggct gttcaggctg cggctgatag gcaggctgct gcatcggctg cgctggacgc
     5401 ggagcatgtt gcggcggtac ctgcgcttca ggcggctgct ggatcggttg acgcggctgc
     5461 gcagacgcat aaggcggttg gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc
     5521 tgagcgttag ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca
     5581 tcttcgacat cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc
     5641 cgatcgcgga acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa
     5701 gcgattatgg cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata
     5761 ttctctgttg ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc
     5821 cgattacctc aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt
     5881 gctttttgct gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat
     5941 acgcagaatt ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc
     6001 acatctgccc cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa
     6061 cctgggattc ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc
     6121 gcattctggt ggctctttac acagctcgat gtctggatcc cgactctcat gagttacgtt
     6181 ccggactggc tacaatggct gagttatctg ttgtggcctc tggctgtcat ctctgtgctg
     6241 ttagtgtttg gctatttctt ctccacgatt gctaactgga ttgccgctcc gtttaacggt
     6301 ttattggctg aacaactgga agcacgattg actggcgcta caccgccaga taccgggatt
     6361 ttcggtatca tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgcctgg
     6421 tatctgccgc gcgcaattgt attactaatt ctttacttca tccctggtat tgggcaaacc
     6481 gtcgcgccgg tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgcgat
     6541 taccccttcg ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc
     6601 aaaatcacca atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt
     6661 aatctgttca tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat
     6721 cgcgataaac acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt
     6781 gaaacagggg tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg
     6841 tatatagata tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg
     6901 tatcccaatt tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct
     6961 gactatcggt cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc
     7021 gaaggtggaa tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat
     7081 ttgggatgcc gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccgaccag
     7141 cggtaatacc gggattgcac tggcctatgt agctgccgct cgcggttaca aactcaccct
     7201 gaccatgcca gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa
     7261 cctggtgctg acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat
     7321 tgtcgccagc aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc
     7381 tgaaattcac gaaaaaacca ccggtccgga gatctgggaa gataccgacg gtcaggttga
     7441 tgtatttatt gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa
     7501 aggcaccaaa ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt
     7561 tatcgcccag gcgctggcag gtgaagagat taaacctggc ccgcataaaa ttcagggtat
     7621 tggcgctggt tttatcccgg ctaacctcga tctcaagctg gtcgataaag tcattggcat
     7681 caccaatgaa gaagccattt ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc
     7741 aggtatctct tctggagcag ctgttgccgc ggcgttgaaa ctacaagaag atgaaagctt
     7801 taccaataag aatattgtgg ttattctacc atcatcgggt gagcgttatt taagcaccgc
     7861 attgtttgcc gatctcttca ctgagaaaga attgcaacag taatgccagc ttgttaaaaa
     7921 tgcgtaaaaa agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc
     7981 ctggcattga ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg
     8041 aaattaatcg ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt
     8101 cctttaacgg cataatgttt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa
     8161 tggactgtat tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg
     8221 ctaaagtcga accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga
     8281 aatacaatgt tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct
     8341 gctgcccagt ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac
     8401 ggcaaaagcg ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt
     8461 accgttgtga ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt
     8521 aaactgatgg cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag
     8581 gtagggttat gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc
     8641 ttctgaaaga agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac caggttgatc
     8701 aggaagttga acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca
     8761 aaacgaaagc tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta
     8821 tgctgctcga agatgaggag ctggagcagg aaatcatagc cctgattaaa gataaacaca
     8881 tgacagctga cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc
     8941 tggatgatga atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc
     9001 tgcgcaacat cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc
     9061 tggttgccgc tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg
     9121 gtttcatcac cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg
     9181 aactgcctgc tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc
     9241 tgattctgga tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata
     9301 aaatgcgcgc tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag
     9361 atctgccagc tattacgctg gacggtcacc aggtagaagt atgcgctaac attggtacgg
     9421 ttcgtgacgt tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg
     9481 agttcctgtt catggaccgc gacgcgctgc ccactgaaga agaacagttt gctgcttaca
     9541 aagcagtggc tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg
     9601 gcgacaaaga gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc
     9661 gcgctatccg tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc
     9721 tgcgtgcctc ggctttcggt aaattgcgca ttatgttccc gatgatcatc tctgttgaag
     9781 aagtgcgtgc actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta
     9841 aagcgtttga cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa
     9901 ttgcacgtca tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc
     9961 agtacactct ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt
    10021 caccgtccgt gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat
    10081 ggactggcat gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga
    10141 tgggtctgga cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc
    10201 gtaacacgaa cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa
    10261 cggacgagtt aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca
    10321 cgagatgcgg cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa
    10381 tctctggttt ccgacgacaa gaaggatacc ggaactattg agatcattgc tccgctctct
    10441 ggcgagatcg tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt
    10501 gatggtattg ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt
    10561 ggtaaaatct ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg
    10621 ttcgtccact tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct
    10681 gaagaaggtc agcgcgtgaa agttggcgat actgtcattg aatttgatct gccgctgctg
    10741 gaagagaaag ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa
    10801 gaactgatca aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag
    10861 aagtaattct tgccgcagtg aaaaatggcg cccatcg
//
LOCUS       DAJVYM010000128        10734 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVYM010000128 DAJVYM010000000
VERSION     DAJVYM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.129"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            61..783
                     /locus_tag="OQS60_003105"
                     /pseudo
     CDS             61..783
                     /locus_tag="OQS60_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            793..1272
                     /locus_tag="OQS60_003106"
     CDS             793..1272
                     /locus_tag="OQS60_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388798.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1409..2194)
                     /locus_tag="OQS60_003107"
     CDS             complement(1409..2194)
                     /locus_tag="OQS60_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388799.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2523..2599)
                     /locus_tag="OQS60_003108"
     tRNA            complement(2523..2599)
                     /locus_tag="OQS60_003108"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2563..2565),aa:Asp,seq:gtc)
     gene            complement(2732..3463)
                     /gene="dnaQ"
                     /locus_tag="OQS60_003109"
     CDS             complement(2732..3463)
                     /gene="dnaQ"
                     /locus_tag="OQS60_003109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS2388800.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3528..3995
                     /gene="rnhA"
                     /locus_tag="OQS60_003110"
     CDS             3528..3995
                     /gene="rnhA"
                     /locus_tag="OQS60_003110"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS2388801.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3992..4714)
                     /locus_tag="OQS60_003111"
     CDS             complement(3992..4714)
                     /locus_tag="OQS60_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS2388802.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4748..5503
                     /gene="gloB"
                     /locus_tag="OQS60_003112"
     CDS             4748..5503
                     /gene="gloB"
                     /locus_tag="OQS60_003112"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS2388803.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5575..6933
                     /gene="mltD"
                     /locus_tag="OQS60_003113"
     CDS             5575..6933
                     /gene="mltD"
                     /locus_tag="OQS60_003113"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS2388804.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6982..7578)
                     /locus_tag="OQS60_003114"
                     /pseudo
     CDS             complement(6982..7578)
                     /locus_tag="OQS60_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(7609..8409)
                     /locus_tag="OQS60_003115"
     CDS             complement(7609..8409)
                     /locus_tag="OQS60_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS2388805.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8650..9564
                     /locus_tag="OQS60_003116"
     CDS             8650..9564
                     /locus_tag="OQS60_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388806.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9561..10364)
                     /gene="dkgB"
                     /locus_tag="OQS60_003117"
     CDS             complement(9561..10364)
                     /gene="dkgB"
                     /locus_tag="OQS60_003117"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS2388807.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10527..10603)
                     /locus_tag="OQS60_003118"
     tRNA            complement(10527..10603)
                     /locus_tag="OQS60_003118"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10567..10569),aa:Asp,seq:gtc)
BASE COUNT         2983 a         2448 c         2426 g         2877 t
ORIGIN      
        1 accgatctcg tatgccgtct tctgcttgaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
       61 aaaaaaatac tcagcccaat aacatcattt gattacattc attttatcac tacacatcct
      121 tcaggtataa aagatacact cgcctggtta gttaacgcga gtaaattaat gactgagttt
      181 gatgacaatg ggaaaattat atttaacatt aatgccttaa agtatacaaa agcaagttac
      241 tttgaaatac ttggagagaa atatataaaa atcacaacat ccagcccctg gcttttagaa
      301 aagctcggta aatatatttt ctcttcacga gcgcctcaag ttctggaact cgctattggc
      361 tggcgaggag ctttatctga atcaataaaa ggagttaagt tctgtatctg gttttcagta
      421 gcctggagaa ccatagaatt tattatgagt tcagagcgag atctggttaa ttttcttggc
      481 gatttttcaa tggatgtggc caaagcagtt atagccggtg gtgttgcaac cgctattgga
      541 agcctggctt cttttgcctg tgttagcttt ggctttccag taattcttgt cggaggagca
      601 attttactga cagggatagt gtgtacagtt gttttaaatg aaatcgatgc tcaatgccat
      661 ttatcagaaa aattaaaata tgcaattaga gatggactaa aacggcaaca ggaacttgat
      721 aaatggaaaa gggaaaacat gactccattt atgtatgttc ttaacactcc acccgtgata
      781 tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt ttctttcgat gttttttatg
      841 gccatattat ttttccctgc attcaatgct tctttgtttt ttaccggagt taaacctcta
      901 tattcaataa taaaatgctc cacggaaata ttttatgatt ggagaatgtt gattctgtgc
      961 tttggtttca tgtcgttttc ctttttaaat attcatgtaa ttttacttac gataataaaa
     1021 ttattcttaa tcaaaaaaac aaaggtagtc aatttcgcca cagacataac aatacagcta
     1081 actcttatat ttttactaat agctatcgta atagcacctt taatagctcc ttttgtcaca
     1141 ggatatgtca atgctaatta tcacccctgc ggtaataata cgggaatttt cccaggagca
     1201 atctatatta aaaatggcat gaaatgtaat aatgaatata tttctcgcaa ggaagattca
     1261 gcagttaaat aattaaacat tctaaaacaa acagttttta actccggatt ccatgtcctt
     1321 tcttgcactg tctttgtcaa gaaaaatttc ttatgcaaag aattttgtat tatttccgcc
     1381 agaaattcct ggcggcagat ataatttatt attgatagcg gttaaccaaa tcttttacca
     1441 tcgctttata ggtcttttgc ttaagttgca tgatttgctg ctctgtcata tcagtcgtac
     1501 tggcttcatt cttgtcgggc aatggtggaa gcactttata ttcataattc accggcgacc
     1561 aggcagccca ctcacctgta gctacatcca ccagcgtgaa tcgtaccaga tagcgcagtg
     1621 acatcgtgtc agtcaatttt tgatttttat aatctgacca aactattgat ttcaatccag
     1681 agtcatattt tcctgtctgc aacgtatcct ggaaaacaat aatcgctttc tgatgccctt
     1741 ttgcagccac aaaacgcagt gcctgcatcc agttcatatt ctcagcactg gcaacatcct
     1801 cgttctcatt tttatctttg tttttgttac aagtcagagg cttttgcctg tccgggatac
     1861 cagagaatgt ggaaacagta taatattttc gcatctcctc ctgcataatg gtttccggtg
     1921 cgcggttgcc ggattgaaca agtattacag gagaattaag gggaacgcgg aactcatctc
     1981 catcaagcgc agcttgaata tcctcttcgg atacggccag agtggtttca ttaccgaata
     2041 aatctttgtc tgtcagttgc tggggagtgt ttttattaat attactttgt gtgacttgag
     2101 cttttttctt cttttcatca atagaagatt cagagacaca tcctgcaagc agcgagaata
     2161 atacacatat acaacaaacc cttcttaaat tcataatcgg acactccctc gcctgtcata
     2221 atcatggtaa tgtttagcta acgattttta attatatact ccacttgaat cagaacaaac
     2281 aaaaataaca gtatatataa attcattaaa tcaatagaaa aatgaaaatc ataacattca
     2341 aatatattta aattgaatga tttgatagta tgattaaata ttaatgatat ggtctaccat
     2401 atgtgattaa cacatttaca gcataacaaa accatagacc gaagaaatag cgggatttaa
     2461 atgcaaaaaa ccgcaaaatt aaaaatcttg cggttctctg aactcatttt catgagtgaa
     2521 tttggcggaa cggacgggac tcgaacccgc gcccccctgc gtgacaggca ggtattctaa
     2581 ccgactgaac taccgctccg cgttgtgttc cgttgggaac gaggcgaata ttacggattg
     2641 cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca
     2701 agccgctatt tttagcgcct tttacaggta tttatgcccg ccagaggcag cttccgccct
     2761 tcttctccac cagatcaaga cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa
     2821 caacgcgtaa cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct
     2881 gctgtgtctc tccttccatc gcaaaagcca tcgacgtttg accaccggtc atcgccagat
     2941 aaacttccgc aaggatctgt gcatcgagta atgccccgtg tagcgttcgt ttactgttat
     3001 ctatttcgta gcgggcacat aacgcatcga ggctgttgcg cttaccagga aacattttcc
     3061 tcgccaccgc aaggctatcg gtgaccttac agaaggtatt ggtcttcgga atatcgcgct
     3121 taagcaacga aaactcgtag tccataaagc cgatatcgaa cgctgcgtta tggatcacca
     3181 actccgcgcc gcggatatag tccatgaact catcggctac ttcggcaaac gtgggcttat
     3241 cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc ttccggatcc accagccgat
     3301 cgggtttgag ataaacatgg aagttattgc ccgtcaggcg acggttcacc acttcaacgg
     3361 caccaatctc aatgatcttg tggccttcat agtgcgcacc aatctggttc ataccggtgg
     3421 tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc atttatgtca
     3481 gacttatcgt tttacagttc gattcaatta caggaagtct accagagatg cttaaacagg
     3541 tagaaatttt caccgatggt tcgtgtctgg gcaatccagg acctgggggt tacggcgcta
     3601 ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc accaccaaca
     3661 accgtatgga gttgatggcc gctattgtcg cgctggaggc gttaaaagaa cattgcgaag
     3721 tcattttgag taccgacagc cagtatgtcc gccagggtat cacccagtgg atccataact
     3781 ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc gatctctggc
     3841 aacgtcttga tgctgcccta gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg
     3901 ccggacaccc ggaaaacgaa cgctgtgatg aactggctcg tgctgcggca atgaatccca
     3961 cactggaaga tacgggctac caagtcgaag tttaagcttg tggtttacga cattgccggg
     4021 tggctccaac cgcctggcga attcgtggct tgtttttact ctgtttcatc gggtttagcg
     4081 ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat
     4141 gcgcattcaa tagttttcct ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt
     4201 gcagcacttc aaaattcaac aaagagagcc agtccagttg ccgcatcaga gtaaacatcc
     4261 ggctgttata gggcgaggtt tttcgcaata ccggcacaag tttgcgtaat cccattaaac
     4321 taatgggatt gaagccacta atcaccagcc agccatcatc aatcaatacc cgatcggttt
     4381 cacgcaataa acgatgcgga tcggtgcacc acggcaatgt atgtgccagt agacaaacat
     4441 caacggattt atcggcaaaa ggaagatgaa gtggatccgc ctgcacctgg acgggcattc
     4501 cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc
     4561 ttaaattgcc aatcttaagc agatgaaaac catacatttt agtgaaccac gggttgagct
     4621 ggcgctccag cgccttgcga taaagctctc cccagggcaa atcgccccag cgatcaggag
     4681 ccacgacagt ttgagggact cttgccggtt tcatcacaac cttccgtttc acactaagag
     4741 gtaatctatg aatcttaaca gtattcccgc ctttgatgac aattacatct gggttttgaa
     4801 tgatgaagca ggtcgctgcc tgattgtcga tcccggagac gcagagccag tattaaacgc
     4861 cattgccgcc aataactggc aaccggaggc catatttctc acccaccatc atcacgatca
     4921 cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt atggtccaca
     4981 agagacacaa gataagggaa caacacaggt agtcaaagat ggcgaaactg ccttcgtttt
     5041 ggggcatgaa tttagtgtaa ttgccacacc gggtcacact ttaggacata tctgttactt
     5101 cagtaaacct tatctatttt gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt
     5161 tgaagggaca gcatcacaaa tgtatcaatc acttaataag ttaagtgcgt tacctgacga
     5221 tacattggta tgttgtgctc atgaatatac gttatcaaat atgaagtttg ctttgagtat
     5281 tcttccgcac gatttgtcca taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa
     5341 aaatcaaata acactacccg taattctgaa aaatgagcgg caaattaatg tttttttaag
     5401 aacggaagat attgatttaa ttaatgtaat taatgaagaa acattattgc aacaacctga
     5461 agagcgtttt gcatggttaa ggtcaaagaa agataggttc tgataaaact ttcttgtcat
     5521 cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa ctattgacac acacatgaag
     5581 gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt
     5641 caacagcacg cacagagcct ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt
     5701 caggcacgat ggatggacga tgggacgtct atcgcgccag atggtgactt gtgggctttc
     5761 attggcgacg agctaaagat gggaattccg gaaaatgacc ggattcgcga acagaaacag
     5821 aaatatttac gcaataagag ctatctccac gatgtaactt tacgggcaga gccgtatatg
     5881 tactggatag cagggcaagt taaaaaacgt aacatgccta tggaactggt actactaccc
     5941 atagtggaga gcgcttttga tcctcacgca acgtctggcg ccaatgccgc aggcatctgg
     6001 cagatcattc cgagcacggg gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg
     6061 cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaata tgatgcagcg tctgaacaaa
     6121 atgtttgacg gcgactggct tctgaccgta gcggcttata acagcggcga aggtcgggtc
     6181 atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg gtcgttaccg
     6241 ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg cattgagtga tattctcaaa
     6301 aacagcaagc gttatggcgt acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt
     6361 gtgcacctga gtagcccggt tgaaatggcg aaggttgcag atatggcggg gatttccgtc
     6421 agcaagctga agacattcaa cgctggcgtg aaaggctcca cgctgggcgc aagtgggccg
     6481 cagtacgtga tggtgccaaa gaagcatgca gatcaactgc gtgaatctct cgcttcaggc
     6541 gaaattgctg ctgtacagtc gacgctggtt gccgacaata cgccgcttaa cagccgtgtt
     6601 tacaccgtac gctctggcga cacgctttca agcatcgctt cacgtctcgg cgtaagcacc
     6661 aaagatttgc agcagtggaa caaactgcgc ggatctaagc tgaagccagg ccaaagtttg
     6721 acgattggtg caggcagtag cgcacagcgg ttggcaaaca acagcgatag cattacgtat
     6781 cgtgtgcgca aaggcgattc gctttcaagc attgctaaac gccacggcgt gaacatcaaa
     6841 gatgtgatgc gctggaacag cgacactgcg aatctgcaac caggcgataa gctgacgttg
     6901 tttgtgaaaa acaacaacat gccagattcc tgacaaacca gataataaaa aaggcaccga
     6961 ttcccccggt gcctttttta tttatgccgc tttatgtgct tctaccatga tgatatcact
     7021 ggtgaaagag ccatcagtct gcaaggaaaa atacgttctc acctctgtcg atgcgctctg
     7081 ttggtaaatg cgaatagcgt ctactaacgc ttctggcgta cgcattctca cgacccatga
     7141 agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat tggcttcatt
     7201 gattaacgtc aaccacgcac cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc
     7261 ttctaccgcc tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta catccatcac
     7321 aatcagccta ccgccaggtt tcaatatcct attcacttct cgcagtgctg caccaacatc
     7381 atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttat cggcaaatgg
     7441 cagacttgcg gcatatccct ggcgggtggt gatatttttc agttgccggg cttcggcggc
     7501 ttgtgccacg acatccagca tgtgggcaga taagtcatac gccaccaccg cgctcacgtt
     7561 ttgcgcagcg ccaaacgttg gtctgccctg cggcagacct gacatacctt atttatcagg
     7621 cttgccggga ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac
     7681 agaagcttca ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc
     7741 ccgacggcgc tgatcatcgg taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg
     7801 atataacgcg ttcatccttc tacggctcca ggcattgaaa tctcccgcca taatgaccgg
     7861 gccgctgtgg tgagctatct gatcaccaat aggaagtaac tgcttactat agacatccac
     7921 gcccagactg aagttgacgg cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa
     7981 tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa
     8041 cgggcagcaa tacactggat gtgccgccga aagggtcatt acgccagaag gatgttgtgg
     8101 cagcacgaaa gcgggtacct gatcggcggc aagatagtta gcggtcgcaa actgtaccaa
     8161 ctctggcgtt gtttgcgctt cctgcaataa caccagatgt gcgtctttgc cgtaattctt
     8221 taataccgac aaccattcag cgcgttgctg tttgtatatg ttccacacca ggatccgaat
     8281 acgctcttcg gtacttaacg gttctccggg tggtaatgcc tggccgatgc tcgcaaaaga
     8341 ccccggcggt aagatcctct ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt
     8401 ttttcgcact tttgactttg aaacctctat tacgtcaacc cttccgcact cgggagggtt
     8461 ctactgttat agggatttta gctcactctt tcaacgagca attgtatatt gttatgtaag
     8521 caagtgctta caccggtcag aaatttgctc attattgaga acattaaatc ggatgacata
     8581 tcacgcatcc tgatggttta atgacaaata aattcaaaat atctcatcta ttttgcaaca
     8641 ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc
     8701 ggcagcttta gccgggcagc ggaacaatta gggcaagcaa actcagcggt aagccgggcg
     8761 gtgaaaaagc tggaaatgaa acttggcgtt agcctgctta atcggaccac gcggcaactt
     8821 agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg
     8881 gcagcggcag aatcagaaat tatggagacg cgtaatacac cgcgtggact gttacggatc
     8941 gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt taattaagcc tttccgtgaa
     9001 cgctatccgg aagtcacttt atcgctagtc tcctccgaaa cgattattaa tttgatcgaa
     9061 agaaaagtgg atgtcgcgat acgcgctggt acgttaacgg actccagctt acgtgccagg
     9121 ccgttattta acagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg
     9181 aagccagaaa cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct
     9241 tccctcaata cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt
     9301 ttgtcatcca atagtgggga aacactgaaa cagctttgcc tgagtggcaa cgggattgcg
     9361 tgtttgtccg actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg
     9421 gcagataaag tgttgccagt ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg
     9481 gtaagtacgc gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc
     9541 ggaggagctg tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg
     9601 cggtcgttgc aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt
     9661 tgtgccttaa gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa
     9721 ccttccccca tagcccacgc cagaatcact tgtgccggag tcgcattgtg tttagctgca
     9781 atacgagcaa taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg
     9841 atatgaatac cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag
     9901 agttcaatct ggttggtagc gatgttttca gcgccaacag cagcaatcgc tttttccatc
     9961 aacgggatcg tgaagttgga aataccgatc tcacgtgtca gaccttgttt tttggcctcc
    10021 agcagcgcct gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt
    10081 agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctcttttag actcgggatc
    10141 aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt
    10201 ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgca
    10261 gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg
    10321 tctttgagac ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa
    10381 tgctattttc gttaactgag gtgattatgg gcttcagact gttgcggaaa aagagcataa
    10441 aatgcagagg atttttgcga ttctggcaat aatagatata cagacgaaaa aaaacctcga
    10501 gcgttaactc gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc
    10561 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
    10621 ccaccggttt tatgaccggc ttactgctta atttgatgct tggcagttta tgaatcactt
    10681 cgggctgttg cttcgcaacg ttcaaaagct ttcgcttttg tcctactctc ttat
//
LOCUS       DAJVYM010000129        10700 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVYM010000129 DAJVYM010000000
VERSION     DAJVYM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.130"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQS60_003119"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQS60_003119"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS2388808.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQS60_003120"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQS60_003120"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS2388809.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OQS60_003121"
     CDS             complement(2696..3388)
                     /locus_tag="OQS60_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCS2388810.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OQS60_003122"
     CDS             3518..5278
                     /locus_tag="OQS60_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS2388811.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OQS60_003123"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OQS60_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS2388812.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OQS60_003124"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OQS60_003124"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS2388813.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OQS60_003125"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OQS60_003125"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS2388814.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9903..10079)
                     /locus_tag="OQS60_003126"
     CDS             complement(9903..10079)
                     /locus_tag="OQS60_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCS2388815.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10179..>10700
                     /locus_tag="OQS60_003127"
     CDS             10179..>10700
                     /locus_tag="OQS60_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388816.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYF"
BASE COUNT         2753 a         2677 c         2538 g         2732 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc
     5461 ccttttaatt aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat gtttactccg tatctgcata aaaaccatgc
     5581 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agccgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatccccg gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaat ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgca gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg
     9241 aaagaggtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgagg caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gagcttctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc
     9901 atctaccata tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg
     9961 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt
    10021 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
    10081 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac
    10141 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
    10201 ttgccacttt ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
    10261 gcttatcctc gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc
    10321 gcttattcaa tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg
    10381 aacgattgcg cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc
    10441 gtctgacacc aacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt
    10501 taatgccttt actggttggt ttcacacgtg aatatcctga tattaaagtc gagctcacaa
    10561 ctgacgactc tcttgtcgat atcgtccaac agggctttga cgctggcgtt cgacttagtg
    10621 gcatcgtcga aaaagatatg atcagcgtgg cgatcggccc accagtgaaa ttatgtgtag
    10681 cagcaacgcc agagtacttt
//
LOCUS       DAJVYM010000130        10687 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVYM010000130 DAJVYM010000000
VERSION     DAJVYM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.131"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..784)
                     /locus_tag="OQS60_003128"
     CDS             complement(<1..784)
                     /locus_tag="OQS60_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388817.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OQS60_003129"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OQS60_003129"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS2388818.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OQS60_003130"
     CDS             1860..2747
                     /locus_tag="OQS60_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS2388819.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OQS60_003131"
     CDS             2780..3790
                     /locus_tag="OQS60_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS2388820.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OQS60_003132"
     CDS             3860..5293
                     /locus_tag="OQS60_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2388821.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OQS60_003133"
     CDS             5392..6741
                     /locus_tag="OQS60_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS2388822.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OQS60_003134"
     CDS             6731..7618
                     /locus_tag="OQS60_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS2388823.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OQS60_003135"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OQS60_003135"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS2388824.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OQS60_003136"
     CDS             8352..9224
                     /locus_tag="OQS60_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS2388825.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OQS60_003137"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OQS60_003137"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS2388826.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="OQS60_003138"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="OQS60_003138"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS2388827.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2533 c         2854 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAJVYM010000131        10648 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVYM010000131 DAJVYM010000000
VERSION     DAJVYM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.132"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OQS60_003139"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OQS60_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS2388828.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OQS60_003140"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OQS60_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS2388829.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OQS60_003141"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OQS60_003141"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS2388830.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OQS60_003142"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OQS60_003142"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS2388831.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OQS60_003143"
     CDS             complement(5254..6294)
                     /locus_tag="OQS60_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2388832.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OQS60_003144"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OQS60_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS2388833.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OQS60_003145"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OQS60_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS2388834.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OQS60_003146"
     CDS             7229..7669
                     /locus_tag="OQS60_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS2388835.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OQS60_003147"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OQS60_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS2388836.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OQS60_003148"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OQS60_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS2388837.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10648
                     /gene="rsxC"
                     /locus_tag="OQS60_003149"
     CDS             8898..>10648
                     /gene="rsxC"
                     /locus_tag="OQS60_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS2388838.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQV"
BASE COUNT         2450 a         2751 c         2720 g         2727 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggt
//
LOCUS       DAJVYM010000132        10482 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVYM010000132 DAJVYM010000000
VERSION     DAJVYM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.133"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OQS60_003150"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OQS60_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS2388839.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OQS60_003151"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OQS60_003151"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS2388840.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OQS60_003152"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OQS60_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCS2388841.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OQS60_003153"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OQS60_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS2388842.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OQS60_003154"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OQS60_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OQS60_003155"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OQS60_003155"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS2388843.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OQS60_003156"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OQS60_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS2388844.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OQS60_003157"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OQS60_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS2388845.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OQS60_003158"
     CDS             complement(9457..10149)
                     /locus_tag="OQS60_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS2388846.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2564 a         2530 c         2773 g         2615 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acaccccgcg ccgctgagaa aaagcgaagc gg
//
LOCUS       DAJVYM010000133        10362 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVYM010000133 DAJVYM010000000
VERSION     DAJVYM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.134"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..154
                     /locus_tag="OQS60_003159"
     CDS             <1..154
                     /locus_tag="OQS60_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2388847.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OQS60_003160"
     CDS             258..1172
                     /locus_tag="OQS60_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2388848.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OQS60_003161"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OQS60_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS2388849.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OQS60_003162"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OQS60_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS2388850.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OQS60_003163"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OQS60_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS2388851.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OQS60_003164"
     CDS             5467..7149
                     /locus_tag="OQS60_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS2388852.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OQS60_003165"
     CDS             7201..8358
                     /locus_tag="OQS60_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS2388853.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OQS60_003166"
     CDS             complement(8324..8446)
                     /locus_tag="OQS60_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388854.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OQS60_003167"
     CDS             8655..10340
                     /locus_tag="OQS60_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCS2388855.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2207 c         2214 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg ta
//
LOCUS       DAJVYM010000134        10264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVYM010000134 DAJVYM010000000
VERSION     DAJVYM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.136"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..611
                     /locus_tag="OQS60_003168"
                     /pseudo
     CDS             1..611
                     /locus_tag="OQS60_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OQS60_003169"
     CDS             608..1222
                     /locus_tag="OQS60_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS2388856.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQS60_003170"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQS60_003170"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS2388857.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OQS60_003171"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OQS60_003171"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS2388858.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OQS60_003172"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OQS60_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS2388859.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OQS60_003173"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OQS60_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS2388860.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQS60_003174"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQS60_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS2388861.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="OQS60_003175"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="OQS60_003175"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS2388862.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="OQS60_003176"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="OQS60_003176"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS2388863.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="OQS60_003177"
     tRNA            9115..9190
                     /locus_tag="OQS60_003177"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="OQS60_003178"
     CDS             9796..9954
                     /locus_tag="OQS60_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388864.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2566 a         2462 c         2650 g         2586 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat taaaatataa acagataatt atgtatctta
    10201 ttgatttata aggttatttt taagtaaagg taaataaaaa ataaatagcc ataaatagct
    10261 ataa
//
LOCUS       DAJVYM010000135         9850 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVYM010000135 DAJVYM010000000
VERSION     DAJVYM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.137"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OQS60_003179"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OQS60_003179"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS2388865.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="OQS60_003180"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="OQS60_003180"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS2388866.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="OQS60_003181"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="OQS60_003181"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS2388867.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="OQS60_003182"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="OQS60_003182"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS2388868.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="OQS60_003183"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="OQS60_003183"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS2388869.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="OQS60_003184"
     CDS             5058..6191
                     /locus_tag="OQS60_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS2388870.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="OQS60_003185"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="OQS60_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS2388871.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OQS60_003186"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OQS60_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS2388872.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OQS60_003187"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OQS60_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS2388873.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OQS60_003188"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OQS60_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS2388874.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OQS60_003189"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OQS60_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS2388875.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="OQS60_003190"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="OQS60_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS2388876.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9850
                     /locus_tag="OQS60_003191"
     CDS             9762..>9850
                     /locus_tag="OQS60_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388877.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2503 c         2502 g         2328 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaatg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg
//
LOCUS       DAJVYM010000136         9819 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVYM010000136 DAJVYM010000000
VERSION     DAJVYM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.138"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OQS60_003192"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OQS60_003192"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS2388878.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OQS60_003193"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OQS60_003193"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS2388879.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OQS60_003194"
     CDS             1829..2101
                     /locus_tag="OQS60_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS2388880.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OQS60_003195"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OQS60_003195"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS2388881.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OQS60_003196"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OQS60_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS2388882.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OQS60_003197"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OQS60_003197"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS2388883.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OQS60_003198"
     CDS             6114..6665
                     /locus_tag="OQS60_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS2388884.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OQS60_003199"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OQS60_003199"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS2388885.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OQS60_003200"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OQS60_003200"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS2388886.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2450 c         2617 g         2318 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg
     1561 gctgtggttg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ccgatggcgt
     1921 tgcgttgacc gaggaacaaa aagcgaactg cctgcaactg gtgatgctgt ggcaagctcg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttaggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgcgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacatt taccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagcgaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg ggtgccagtc ctgactggcg
     2761 ataaccgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
     2821 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgaggggg cattacagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ccggttggcg
     3241 cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggca gtatacgcca tcgtgtcggt
     3661 gccgtgcagg atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcttctggcg tcatatcaga agaatccatc agcggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgacg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgccagc tctgctgctt tggcgaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatctg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtta gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatgtca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaaa tccaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacacaac cggcacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgacggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ttttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attatcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgctcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgctgc agccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 cagccatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gtggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagt
//
LOCUS       DAJVYM010000137         9574 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVYM010000137 DAJVYM010000000
VERSION     DAJVYM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.139"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..158)
                     /locus_tag="OQS60_003201"
     CDS             complement(<1..158)
                     /locus_tag="OQS60_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCS2388887.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPA"
     gene            complement(158..1084)
                     /gene="glsB"
                     /locus_tag="OQS60_003202"
     CDS             complement(158..1084)
                     /gene="glsB"
                     /locus_tag="OQS60_003202"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS2388888.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1148..2536)
                     /gene="sad"
                     /locus_tag="OQS60_003203"
     CDS             complement(1148..2536)
                     /gene="sad"
                     /locus_tag="OQS60_003203"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCS2388889.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2637..3518
                     /locus_tag="OQS60_003204"
     CDS             2637..3518
                     /locus_tag="OQS60_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2388890.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3595..4710
                     /gene="yneK"
                     /locus_tag="OQS60_003205"
     CDS             3595..4710
                     /gene="yneK"
                     /locus_tag="OQS60_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS2388891.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4860..6050
                     /gene="ydeA"
                     /locus_tag="OQS60_003206"
     CDS             4860..6050
                     /gene="ydeA"
                     /locus_tag="OQS60_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS2388892.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6075..6740)
                     /gene="marC"
                     /locus_tag="OQS60_003207"
     CDS             complement(6075..6740)
                     /gene="marC"
                     /locus_tag="OQS60_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS2388893.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6952..7386
                     /gene="marR"
                     /locus_tag="OQS60_003208"
     CDS             6952..7386
                     /gene="marR"
                     /locus_tag="OQS60_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS2388894.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7406..7789
                     /gene="marA"
                     /locus_tag="OQS60_003209"
     CDS             7406..7789
                     /gene="marA"
                     /locus_tag="OQS60_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS2388895.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7821..8039
                     /gene="marB"
                     /locus_tag="OQS60_003210"
     CDS             7821..8039
                     /gene="marB"
                     /locus_tag="OQS60_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS2388896.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8096..9535)
                     /locus_tag="OQS60_003211"
     CDS             complement(8096..9535)
                     /locus_tag="OQS60_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS2388897.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2467 a         2458 c         2162 g         2487 t
ORIGIN      
        1 gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct
       61 ttctccaggc aatactgacg ctctttcatg cccagacgaa agcgagagcg aaattttgaa
      121 cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgccctaact
      181 gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt
      241 ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa
      301 cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac
      361 cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat
      421 caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac
      481 agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat
      541 ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa
      601 attcggaacg cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca
      661 cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca
      721 ccagcgcacc agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca
      781 ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt
      841 cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa
      901 aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc
      961 ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac
     1021 caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg
     1081 ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata
     1141 tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga
     1201 aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt
     1261 cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc
     1321 tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag
     1381 ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca
     1441 tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt
     1501 agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg
     1561 ttttctctac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag
     1621 tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt
     1681 cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct
     1741 gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat
     1801 cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg
     1861 ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag
     1921 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     1981 atacgccttg cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca
     2041 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     2101 cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg
     2161 gtcgatactc aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg
     2221 ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg
     2281 cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg
     2341 cgcgcagagc cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc
     2401 gccagtcgcg aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag
     2461 cccacggcag cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg
     2521 ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt
     2581 tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg
     2641 atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg
     2701 cagcaaaagt gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag
     2761 aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc
     2821 ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg
     2881 tcgctggcga tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag
     2941 tgcgcattcc ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac
     3001 tttccaccgg cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg
     3061 catttattga tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg
     3121 agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg
     3181 gcagtaacat ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt
     3241 ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt
     3301 tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta
     3361 tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa
     3421 cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac
     3481 tggatgtgtc tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg
     3541 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
     3601 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
     3661 gcaccacagg taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt
     3721 gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac
     3781 cttgataact tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat
     3841 gaaaaggttc agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt
     3901 gaatttcagg tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga
     3961 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
     4021 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg
     4081 ttcaaccctg gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg
     4141 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
     4201 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
     4261 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
     4321 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
     4381 gaagtctgca tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac
     4441 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     4501 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat
     4561 atggcgcgca ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac
     4621 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc
     4681 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     4741 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac
     4801 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     4861 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     4921 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     4981 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     5041 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     5101 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     5161 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     5221 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     5281 taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg
     5341 ggcagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc
     5401 ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa
     5461 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     5521 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     5581 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     5641 gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga
     5701 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     5761 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     5821 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     5881 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     5941 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     6001 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     6061 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     6121 tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc
     6181 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     6241 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     6301 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt
     6361 ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     6421 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg
     6481 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     6541 tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     6601 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     6661 agcgcgacag ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     6721 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     6781 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     6841 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     6901 tgcctgggca atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     6961 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     7021 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     7081 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     7141 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     7201 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     7261 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     7321 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     7381 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     7441 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     7501 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     7561 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     7621 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     7681 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     7741 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg
     7801 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     7861 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     7921 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     7981 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     8041 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa
     8101 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
     8161 gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt
     8221 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta
     8281 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
     8341 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
     8401 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
     8461 gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
     8521 ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa
     8581 aagacatgta gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat
     8641 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
     8701 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg
     8761 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac
     8821 agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
     8881 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg
     8941 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
     9001 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
     9061 ttgatgcgaa gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg
     9121 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct
     9181 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
     9241 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
     9301 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
     9361 tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc ccgtgagcgc
     9421 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
     9481 actgatgcgc ggctaccgcg ccaccccata aaaaaccttt tttaaatcct gacatacaca
     9541 actccttaac taaaatagat taccaccaga cttc
//
LOCUS       DAJVYM010000138         9542 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVYM010000138 DAJVYM010000000
VERSION     DAJVYM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.140"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1321
                     /locus_tag="OQS60_003212"
     CDS             <1..1321
                     /locus_tag="OQS60_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2388898.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="OQS60_003213"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="OQS60_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCS2388899.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /locus_tag="OQS60_003214"
     CDS             2703..3482
                     /locus_tag="OQS60_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCS2388900.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="OQS60_003215"
     CDS             3479..6418
                     /locus_tag="OQS60_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCS2388901.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="OQS60_003216"
     CDS             6351..6665
                     /locus_tag="OQS60_003216"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS2388902.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="OQS60_003217"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="OQS60_003217"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="OQS60_003218"
     CDS             complement(7120..7608)
                     /locus_tag="OQS60_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS2388903.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="OQS60_003219"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="OQS60_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCS2388904.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAJVYM010000139         9433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVYM010000139 DAJVYM010000000
VERSION     DAJVYM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.141"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="OQS60_003220"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="OQS60_003220"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS2388905.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="OQS60_003221"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="OQS60_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS2388906.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="OQS60_003222"
                     /pseudo
     CDS             1394..1915
                     /locus_tag="OQS60_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OQS60_003223"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OQS60_003223"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS2388907.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="OQS60_003224"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="OQS60_003224"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS2388908.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="OQS60_003225"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="OQS60_003225"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS2388909.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="OQS60_003226"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="OQS60_003226"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS2388910.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="OQS60_003227"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="OQS60_003227"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS2388911.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="OQS60_003228"
     CDS             complement(6557..7450)
                     /locus_tag="OQS60_003228"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS2388912.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="OQS60_003229"
     CDS             complement(7447..8100)
                     /locus_tag="OQS60_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS2388913.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="OQS60_003230"
     CDS             complement(8111..9370)
                     /locus_tag="OQS60_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS2388914.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2160 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gcctgaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccaatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaacccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cctgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagg taatgactcc
     9421 aacttattga tag
//
LOCUS       DAJVYM010000140         9407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVYM010000140 DAJVYM010000000
VERSION     DAJVYM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.142"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            486..1157
                     /locus_tag="OQS60_003231"
     CDS             486..1157
                     /locus_tag="OQS60_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCS2388915.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OQS60_003232"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OQS60_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OQS60_003233"
     CDS             2363..2923
                     /locus_tag="OQS60_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS2388916.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OQS60_003234"
     CDS             2925..3698
                     /locus_tag="OQS60_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCS2388917.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OQS60_003235"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OQS60_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCS2388918.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OQS60_003236"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OQS60_003236"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCS2388919.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OQS60_003237"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OQS60_003237"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS2388920.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVLWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OQS60_003238"
     CDS             complement(6457..6996)
                     /locus_tag="OQS60_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCS2388921.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OQS60_003239"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OQS60_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCS2388922.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..>9407)
                     /gene="dmpG"
                     /locus_tag="OQS60_003240"
     CDS             complement(8596..>9407)
                     /gene="dmpG"
                     /locus_tag="OQS60_003240"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCS2388923.1"
                     /translation="WIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATH
                     CTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGG
                     AMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMG
                     AGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAG
                     VYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLRNNK"
BASE COUNT         2354 a         2448 c         2281 g         2324 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtact gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccatt
//
LOCUS       DAJVYM010000141         9212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVYM010000141 DAJVYM010000000
VERSION     DAJVYM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.143"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            73..732
                     /locus_tag="OQS60_003241"
     CDS             73..732
                     /locus_tag="OQS60_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS2388924.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="OQS60_003242"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="OQS60_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS2388925.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="OQS60_003243"
     CDS             complement(1860..2570)
                     /locus_tag="OQS60_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS2388926.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="OQS60_003244"
     CDS             3004..3294
                     /locus_tag="OQS60_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS2388927.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="OQS60_003245"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="OQS60_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS2388928.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OQS60_003246"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OQS60_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS2388929.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OQS60_003247"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OQS60_003247"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS2388930.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OQS60_003248"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OQS60_003248"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS2388931.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="OQS60_003249"
     CDS             complement(7538..7837)
                     /locus_tag="OQS60_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS2388932.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="OQS60_003250"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="OQS60_003250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS2388933.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="OQS60_003251"
     CDS             complement(9049..>9212)
                     /locus_tag="OQS60_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388934.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAJVYM010000142         8918 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVYM010000142 DAJVYM010000000
VERSION     DAJVYM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.144"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(38..400)
                     /locus_tag="OQS60_003252"
     CDS             complement(38..400)
                     /locus_tag="OQS60_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS2388935.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OQS60_003253"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OQS60_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS2388936.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OQS60_003254"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OQS60_003254"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS2388937.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OQS60_003255"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OQS60_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS2388938.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OQS60_003256"
     CDS             complement(4827..5036)
                     /locus_tag="OQS60_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388939.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OQS60_003257"
     CDS             5321..5683
                     /locus_tag="OQS60_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS2388940.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OQS60_003258"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OQS60_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..>8918
                     /locus_tag="OQS60_003259"
     CDS             6948..>8918
                     /locus_tag="OQS60_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS2388941.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT"
BASE COUNT         2447 a         2103 c         2204 g         2164 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcacc
//
LOCUS       DAJVYM010000143         8904 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVYM010000143 DAJVYM010000000
VERSION     DAJVYM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.145"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            225..2201
                     /locus_tag="OQS60_003260"
                     /pseudo
     CDS             225..2201
                     /locus_tag="OQS60_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2194..3330
                     /locus_tag="OQS60_003261"
     CDS             2194..3330
                     /locus_tag="OQS60_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS2388942.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3327..5327
                     /locus_tag="OQS60_003262"
     CDS             3327..5327
                     /locus_tag="OQS60_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCS2388943.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5449..5910
                     /locus_tag="OQS60_003263"
     CDS             5449..5910
                     /locus_tag="OQS60_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS2388944.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5951..6421)
                     /locus_tag="OQS60_003264"
     CDS             complement(5951..6421)
                     /locus_tag="OQS60_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS2388945.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6468..7187)
                     /gene="yehT"
                     /locus_tag="OQS60_003265"
     CDS             complement(6468..7187)
                     /gene="yehT"
                     /locus_tag="OQS60_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS2388946.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7184..8869)
                     /gene="btsS"
                     /locus_tag="OQS60_003266"
     CDS             complement(7184..8869)
                     /gene="btsS"
                     /locus_tag="OQS60_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS2388947.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT         2126 a         2379 c         2308 g         2091 t
ORIGIN      
        1 agcggccacg atacgtattg attgaaggcc cggctgattt taatgaccgg gtagacgaac
       61 tgtttttagc ccaccagctt ccggtagcta tttacagtta ttgccagtat caggacggtg
      121 cagcccccgg gcgtggtgcc tggacgccat ttgctgaatt ttcgccggag tggcaggcgc
      181 tacaagccgc acgtcgtatt caggcacaaa cttacttcat cgatttgcct tgctgggcgc
      241 agagtgaaga agtggacgat tcgcctgata cgcaagagga aagccaggcc ttgctgctgc
      301 gtgccacccg catggataac agcgacaccc tgtgggatca cttgttcgaa gatgaaagcc
      361 agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg ggcgattccc
      421 ccggcgatgc actcaatcgt cagcgcgaag cctttatggc ccgctggatt ggatgggcga
      481 tgcagcaaaa caatggcgac gtgttagtcg tctgcggtgg ctggcacgct cctgcactgg
      541 caaaaatgtg acgcgaatgc ccgcaggaca ttaacacgcc ggaattgccc tcgctggcag
      601 atgccgttac aggttgttat ctcaccccct acagtgaaaa gcgccttgat gtgctggcag
      661 gatacctttc cggtatgcct gccccggtct ggcaaagctg gtactggcag tggggcttac
      721 agcaggcggg agaacaactg ctaaaaacgg ttcttacccg tttgcgccag cacaaattgc
      781 ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcgctg gcacagttgc
      841 gcggacataa actaccgtta cgcactgact ggctggatgc catagcaggc tcgctgatta
      901 aagaagccct gaacgcgccg ttgccgtgga gctatcgcgg agttattcat cccgacaccg
      961 atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt aaacttgccc
     1021 cttctacgcc acaaccgcct ctgccaaaag atgtcacctg cgaactggaa cgtaccgcaa
     1081 tctctcttcc ggcggagctt accttaaatc gctttaaccc caatgggcta gcgcaaagtc
     1141 aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag cagggaagca
     1201 cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg cttagccagc
     1261 atgcggcatt gattgaggcc gcctgctttg gtgccacact ccaggaagcc gcacgccata
     1321 aattagaagc cgatatgctg gacgcgggtg gaatcggcag tatcaccaca tgtcttagcc
     1381 aggcggcgtt agcgggtctg gcgtccttca gtcaacaatt actggagcaa ctcacattat
     1441 taatcgccca ggaaaatcaa tttgccgaaa tgggccaggc gctggaagtg ctatatgcct
     1501 tatggcggct ggatgaaatt agcggtatgc aaggcgcgca gatattacaa acgacgttat
     1561 gcgcggctat cgatcgcacg ctgtggctgt gtgaatctaa cggcagacca gatgaaaagg
     1621 agtttcacgc tcacctgcat agctggcagg cgctttgcca tatcctgcgc gatctacata
     1681 gcggcgttaa tttacccggc gtttttcttt ctgcggcggt agccttactg gagcgccgca
     1741 gtcaggcgat tcatgtcccg gcgctggatc gcggcgcggc tcttggcgca ctgatgcgtc
     1801 tggaacatcc caacgccagt gccgaagcgg cgctgacgat gctggcgcag ttatccccgg
     1861 cacaatccgg cgaggcgctg cacggtttgc tggcgctcgc ccgtcatcaa ctggcctgcc
     1921 aaccagcatt tatcgccggt ttcagtagtc atttaaatca actgagtgac gacgatttta
     1981 ttaacgccct gcccgatttg cgcgcggcga tggcctggct accgccacga gaacgcggga
     2041 cactggcgca tcaggtgctt gagcattacc agcttgcgca gcttcctgtt tcagcgctgc
     2101 aaatgccgtt gcattgtcct ccgcaagcca ttgcacatca tcaacaactc gaacagcagg
     2161 cactggcatc gctgcaaaac tggggagttt tccatgtctg aactgaacga tcttctgacc
     2221 acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac gacgctttgt
     2281 gggctggatg acaacgcccg gcagatagac cacgcgctgg aatggctgta tgggcgcgat
     2341 cctgaacggc tccagcgtgg tgaacgctcc ggtggattag gtggctcaaa tctcaccact
     2401 cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga gcggctggaa
     2461 agcgatgccg tactgcgcta cggcattgaa gatgtggtga cgaatctcga catgctggaa
     2521 cgtatgcagc cttctgaaag cctgctacgc gccgttttgc acaccaaaca tctgatgaat
     2581 cccgaagtac tggctgccgc ccgccggata gtgcgccagg ttgttgaaga aattatggct
     2641 cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgccg tcgccgctca
     2701 tttattccac tggcgcgaaa ctttgatttc aaaagtactc tgcgcgctaa cctgcaacac
     2761 tggcacccgc aacacggcaa gttgtatatc gaatcccccc gctttaacag ccgcattaaa
     2821 cgccaaagcg aacaatggca actggtctta ctggttgatc aaagcggatc gatggtggac
     2881 tcggtgatcc actctgcggt gatggcggcc tgtttgtggc agttacccgg cattcgtacc
     2941 catctggtgg cgtttgacac aagcgtggtt gatctcacgg cagacgttgc cgatccggta
     3001 gagttattaa tgaaagtaca gttgggcggc gggaccaata tcgccagtgc cgtggagtat
     3061 ggtcggcaac ttattgaaca accagcaaaa agcgtcatta tcctcgtgag cgatttttac
     3121 gaagggggtt catcatcatt gctgacgcat caggtgaaaa agtgtgtcca gagcggcatc
     3181 aaagtgctgg gactggctgc gctcgatagc accgcaacac cttgctatga ccgcgatacg
     3241 gcccaggcgc tggttaatgt cggcgcacaa atagccgcca tgacgccggg cgagctggca
     3301 tcatggcttg cggagaatct tcagtcatga attcactacg tccggaatta ttagaactga
     3361 caccgcaggc cctgacggcg ttaagcaatg ccggttttgt taagcgcagt cttaaggaac
     3421 tggaaaatgg caacgtcccg gagatcagcc atgagaacga cgctttaatc gccaccttca
     3481 gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct cagtgcagtt
     3541 gcggggccaa cggtatgtgc cgtcatcgcg tgatgctggt gttaagttat caacgacttt
     3601 gtgccaccac tcagtctacg gaaaaagaag aagagtggga tccggcaatc tggctggaag
     3661 aactggctac ccttcccgat gctacccgca aacgcgcaca ggcgctggtc gctaaaggca
     3721 tcaccattga gttgttctgt gcgccgggtg aaattccctc tgcccgctta ccgatgagcg
     3781 atatgcgttt ttattcccgc agcagtattc gtttcgcccg ttgtgattgt attgaaagca
     3841 cactttgcga acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa gcgcagcaag
     3901 cggaattgac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca tctgacgatc
     3961 cgtttgccag cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta agccaggcat
     4021 tatggctgag cggcatcagc cagccgctta ttcactacga ggccgctttc agccgcgcgc
     4081 agcaagcagc ggaacgctgc agctggcgat gggtgagtga atcgctacgg caactgcgcg
     4141 ccagcgttga tgccttccac gcccgcgcca gccactatca tgccggagaa tgcttacgtc
     4201 agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg cgcgacagta
     4261 ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt gccggagaag
     4321 caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag gatattgagc
     4381 attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg cacctttcgc
     4441 gcagttggcc gcgaagtgaa caggaaaact caccggcagc tacgcgtcgg ctgtttagtt
     4501 ttcaggctgg cgcactagcg ggcgggcaaa ttgtttcaca agcagcaaaa cgcagtgccg
     4561 atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtgccg ctgtcgcctg
     4621 atgcctggca aatgttgagc gcgccgttac gccagccggg cattgtggct ttgcgggaat
     4681 atttacgcca gcgtcccccc gcctgcatac ggcctcttaa tcaggtcgat aacttattta
     4741 ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag acgctggatg
     4801 cgcaggtaat cagcggcgaa ggtgaagata atctcctgac gttatccctt ccggcatctg
     4861 cctgttctcc ttttgccgtt gaacgcatgg cggcgctttt gcaacaaaca gacgaccccg
     4921 tgagtctggt ttctggcttt gtcagctttg tagaggggca attgacactg gaaccacggg
     4981 tgatgatgac aaaaacccgc gcctgggcgc tggacgcaga aactgcgcct gtggcaccgc
     5041 taccttctgc cagcgttttg cctgtgccgt ctaccgctca tcagttgctg atgcgctgcc
     5101 aggcgttact tattcaactg ctccataacg gctggcgcta tcaggaacag agcgctatta
     5161 gtcaggcaga gttgctggcg aatgacctct ccgcggtcgg tttttatcgg ctggcacatg
     5221 tgttgggaca atttcgtaat acagaaagcg aggcacgggt agaagcaatg aataacggtg
     5281 ttttactttg cgaacaatta ttccccatgc ttcagcaaca aggatgaaat agtgcttact
     5341 aagagttcta ctccagttcc ggactgctca cgccacggta ttaggcatat cctatatagc
     5401 ccctggtgag agtcaccagt tccttgatta aataaaatgg agttttacat gaaggctttc
     5461 aataagctgt tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt
     5521 gacaaagaag aatcgaagaa attcagcgcc aatctgaacg gcactgaaat tgccattacc
     5581 tatgtctaca aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc
     5641 tccattggtg caaccaccaa agaagatgct gccaagacac ttgagccgtt aagcgccaaa
     5701 tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta cgcgcaggaa
     5761 aacgtgacta tcgatatgga aaaagtggat tttaaagccc tgcagggtat ttcaggaatc
     5821 aacgtttctg ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg
     5881 aaagccgctg gttttaaaga agtgaaataa tctgtcggcg gccatgtttc gcatggccgc
     5941 catacccgtc ttagcttttc ttcacatgct ggcgcgctgc cagtccacgc agaaaataac
     6001 gtaaaaattg atcgccgcat tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg
     6061 taatttccgg catcgaaacg cggaactgct gttcggtgag gatcgccaga atgtcatcgg
     6121 ttttcagcga aaacgcgatg cgtaattttt tcagcacgat gttgttatta atgcgacgtt
     6181 ccggctccag tgccggagca gactcatcct tgccgcgttt ttcataaatc aggccattga
     6241 ggaatgacga caaaacaatg tccggacaac gctgaaaacc ctcttcgtct tctttacgta
     6301 gccagacggc gatctgttcc gcggtggctt cgacattacc cagcgccaga atacgcacca
     6361 ggtcattatt attggctttc aaaatgtagc gcacgctgcg cagaatatcg ttacttagca
     6421 tgaggccttc aggtgttaat gaggcaaaaa gccattttag cagtctttta caggccaatc
     6481 gcctctttta agcttttcag ataacggcgg ctgaccggca cggttaagcc attacgcaaa
     6541 atcaactcgg cctggccgtt atcttccaga cgaatctcct gtaaatgcgc gaggttaacc
     6601 agatactgac gatggcagcg cagtagtggt gtacgacttt ccagggtacg taatgtcaat
     6661 tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga caccgctcat ccgactgctg
     6721 acaaatgcca catctttcat ttgcagcaaa taaatccgac tatgccccgt acaagggata
     6781 aatttcagcg cctgttgatt ttccggtaac agcgaaacat cctgcttgct gcgctcctga
     6841 cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa ttggcttcag cagataatca
     6901 aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa acgcagtgag aaaaacaata
     6961 tacgggcgat gttccgggtc aagcatcccc accatttcca gaccactgat gcgcggcatc
     7021 tggatatcga gaaacagcac atccgggcgc agtttatgca ccgcgccgat cccttccacg
     7081 gcgtttgaac actctccaac gatttcaata tcgctctgct cctgcaaaaa tacacgcagg
     7141 ttctcccgtg ctaacggttc atcatcgaca attaagactt taatcatgcc tcgtccctcc
     7201 atggtagtcg taacgttatt cgggtgtaac tatcaggctc acaggcgacg cttattccat
     7261 agtcatcgcc aaaccgttca cgtaaacgct tatccaccag attcatcccc agcccactgg
     7321 cattggttac cggttgatac aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc
     7381 cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag cagttgtgat gtcccatgtt
     7441 taatggcgtt ttccactatc ggttgcaggg taaacgcggg caattgctgc tgggataatt
     7501 cttgcggaat agcaatgttg acctgcaacc gcgactggaa gcgcgccttt tcaatttgca
     7561 gataagcgtt cacatgttca atttcgtcgg cgagagtaac aaactccgaa ggccgcttta
     7621 agtttttgcg gaaaaaagtg gaaagatact gcaccagctg gctggcctgt tcgctgtcgc
     7681 ggcggatcac cgctttaatg gtgttaagcg cattaaacaa aaaatgagga ttcacctggg
     7741 cgtgaagcag tttgatttct gactgggtga gcatcgcttt ttgccgctca tattgcccgg
     7801 caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac
     7861 tgaataaacg gtttttggct tcatacaatt tgatggtgcc catcacgcgc tgattttcgc
     7921 cacgcaacgg aattaccagc gtcgacccca gtttgcattg cggatgcaaa gagcaacgat
     7981 aaggtacttc gttgccatca gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt
     8041 aaggcgaaga aatcggtttg ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca
     8101 gcaatttctc tcgatcggta atcgcgactg caccaatatc cagctcctga tacagcacct
     8161 gagccacttt catgctgttc acttcgttaa acccctgtcg caaaatgcct tccgtcgagg
     8221 ctgccacttt cagcgcagtg gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt
     8281 tatcgagcaa tatacgcata aacagcgccg cgccgacggt attggtgacc atcattggcg
     8341 cagcaatatt actcaccaga cgcaccgcat cttcataagg tcgggcgatc gccaggatga
     8401 tcagcatttg caccatttca gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga
     8461 ctttatcagt gcgcccgcga cggatcagga tgctgtgtac caggccgccg agcaatcctt
     8521 caacgatggt tgagatcatg caacttagcg cggtcatgcc ccccatcgaa tatcgatgta
     8581 agccgccggt cagaccaacc agcccaccga cgaccggacc gccgagtaag ccgcccatta
     8641 ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc
     8701 ccatgatgca gaagatggaa aagacgatgt agcagagaaa tttatgcggc agacgaaccg
     8761 tgacctgcat taacggtatg aataatggcg ttttactcat taaccacgca atgactaaaa
     8821 aaacgcacat ctgctgaagc agcagcaaca ccagattaaa atcgtacata cccgcaaacc
     8881 acacttccct ttaaaacgcg taac
//
LOCUS       DAJVYM010000144         8841 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVYM010000144 DAJVYM010000000
VERSION     DAJVYM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.146"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(149..1231)
                     /locus_tag="OQS60_003267"
     CDS             complement(149..1231)
                     /locus_tag="OQS60_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCS2388948.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="OQS60_003268"
     CDS             complement(1228..3024)
                     /locus_tag="OQS60_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS2388949.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="OQS60_003269"
     CDS             complement(3275..3631)
                     /locus_tag="OQS60_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388950.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="OQS60_003270"
     CDS             complement(3689..4111)
                     /locus_tag="OQS60_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS2388951.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="OQS60_003271"
     CDS             complement(4127..4852)
                     /locus_tag="OQS60_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCS2388952.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="OQS60_003272"
     CDS             complement(4874..5620)
                     /locus_tag="OQS60_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2388953.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6703)
                     /locus_tag="OQS60_003273"
     CDS             complement(5627..6703)
                     /locus_tag="OQS60_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS2388954.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKDTDKED
                     QNTMVHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSL
                     QLQQPEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSA
                     WKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVS
                     GTNNQRCVNHISEPDTEIPPGFRG"
     gene            complement(6775..7200)
                     /locus_tag="OQS60_003274"
     CDS             complement(6775..7200)
                     /locus_tag="OQS60_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS2388955.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7197..7460)
                     /locus_tag="OQS60_003275"
     CDS             complement(7197..7460)
                     /locus_tag="OQS60_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2388956.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7572..8072
                     /locus_tag="OQS60_003276"
     CDS             7572..8072
                     /locus_tag="OQS60_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2388957.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8092..8286
                     /locus_tag="OQS60_003277"
     CDS             8092..8286
                     /locus_tag="OQS60_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS2388958.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8286..8576
                     /locus_tag="OQS60_003278"
     CDS             8286..8576
                     /locus_tag="OQS60_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS2388959.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
BASE COUNT         2256 a         2346 c         1857 g         2382 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctttatct gtatctgtcg tgactcgtcg tgacatgtgc gtgacatttc
     6301 gtgacgcgcc gtgacaatcg ccattttgtt cccgctttct ttccctctct cgctgcgccc
     6361 tcttgcgctc tgcaggagat tttgcggttt gcgaaatatt gccgttgtcc tctttaagca
     6421 cctggcgttt ttcccatcca gtgattaaat caccatcaag tacccgcccc tgcatcgtct
     6481 gcaaaattga atcaattacc tcttctgtca cgtcgagcgc acttgccaaa tcttctgtcg
     6541 tgacatcaat gtgacctcgc gtgacatttc gtgacgcgct caccaggagg tggatataca
     6601 ctgccatcac tgttgcaatt ggctgccctg acaccctggc aattgttcgc cacttagggt
     6661 catttggcat gtcatgccat aatctgagcc aggcgttagc catactcacc tcttctgata
     6721 ccgaatcttt ttactcacga gttgccggga gcgattcgat atggctattg tcagtcaatg
     6781 tactgccaca gcatttcctg ccgggccacc acggttcatc tgattgaaac cggcgattgc
     6841 cactgcgaca aaatcatcag cgtctctcac cagtcgttca tgcgtctcca ccagttcccg
     6901 aaaataagct gaactgtggc tgcgcattcg agccaccagc ggaggtggca ttgccctttc
     6961 gatcgcaggt aacaacgcct gaattttttc aacagcgtca ggggtgtctt tatccagcca
     7021 acggaaaatc ttttgggtgt tgagataaag cgtgttgtgc ttgctgtcgt tatgcagaac
     7081 cggaaacgtc attcccagtt cgaaataaac cctggttatt tcagctgccg gaactttttc
     7141 accgtccgga tgcgcccagg ctttcatcgc catgcggatg tgttcatgct tgattttcat
     7201 gaatcaaccc ccgccgatgg ttgtgtgtta gcctgaaact cagcaggcaa gccgtcagtt
     7261 gggttggggt aggttttact gtcaatctca tgtggggtca ccacccatcc cgttgcttca
     7321 caccaacgta atattttttc tcctgtaagt tttgcacgcc ccgttataac gtggctgacc
     7381 atcccttggg atgcgccaac aatctcagca aatgaactct gggttatatg aaaatgatgc
     7441 aaatattctc caagattcat aatccgccct atgtgatgtg ttaccaatgc ataattaata
     7501 gcactgttat ttttaaaagt aaatagtgat actatttcaa agtgattagc aatcttatta
     7561 gaattggatg tatgaaaaga aaatcattgt cagacaccga cctcaacgcc gccagaagac
     7621 tgaaagaaat ctggatggca aagaaatctc agttgggatt gactcaggaa cgtgcggcgg
     7681 aaattatggg gttttcaaca caaggggcgg taagtcacta cctaaatggt caaaccccac
     7741 taaaccttga ggctgttcta aagtttgctg gcttactgaa agttccccct gagtcaatca
     7801 ggccagacat ggtagatttg ctgcaaattg caaggatgta ttgtcgagaa ccacagcaag
     7861 ataacatagt cgcattacca gcagacacag aacaaacaga ggatgaacta ccgtttgccg
     7921 tagagccgat ggaacgagat ttaatccata cgttcagagc ttttccgaaa gaagatcaag
     7981 aacacatgct tcaggagatg aaagataaaa aagaatcaat ggatcgtacg gttgcgcggt
     8041 ggttagctgc acaaaaaggc cgtcgcgcct aaatcagagg agatatgtaa aatgagtaca
     8101 gtcctttccc caatggtttc agaatttgag acagttgagc aagaaaatag ttacaacgaa
     8161 tggctgcggg ccaaagtagc agcaagcctc gcggatcccc gcccggcgat tccgcatgat
     8221 gaggtaatgg cggaaatgga acaaattatt gcccaaattg ctgctgcaac tcacagaggt
     8281 gaataatgtt acccgtttta tggctaccat ctgctcgtga tgatttgcgc cagatcgtag
     8341 cctacatagc taaagaaaat cccgcggcag cacgcagaat gaaaattcga attgaaacat
     8401 ccgttttgcc gctcacagag catccttacc tgtatcctcc aagcgagcga gttcttggtc
     8461 ttcgtgaaat tgtaactcat cctaactaca tcatccttta ccgagtaaca gcatcaagca
     8521 tagaagtagt aaatattgtg cattctcgaa gacagtatcc aggcaaaaac agttaattat
     8581 tcccgccgac agaaccacct tagggtggtt ttttcttgcc ccaacaaaaa acaatagcga
     8641 tgctatttac atcaaaaaaa ataatgatgc tattgtttca ggcgtcaacc caccccgccc
     8701 cacagaatgc agggcaatac ttcgagttac cagacagtgg tcaggggtta agtagccagc
     8761 ccgaggcgta agaacatgac ggcagggttc aatctttaac tatgcagcag gttttttgtt
     8821 ccgccacccc ggcgttaagg g
//
LOCUS       DAJVYM010000145         8840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVYM010000145 DAJVYM010000000
VERSION     DAJVYM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.147"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            814..1056
                     /locus_tag="OQS60_003279"
     CDS             814..1056
                     /locus_tag="OQS60_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388960.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OQS60_003280"
     CDS             complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OQS60_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS2388961.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OQS60_003281"
     CDS             complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OQS60_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS2388962.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OQS60_003282"
     CDS             complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OQS60_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS2388963.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNADLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OQS60_003283"
     CDS             complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OQS60_003283"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6322..6324),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS2388964.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMTEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7103..7936
                     /gene="fdhD"
                     /locus_tag="OQS60_003284"
     CDS             7103..7936
                     /gene="fdhD"
                     /locus_tag="OQS60_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS2388965.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8089..>8840
                     /locus_tag="OQS60_003285"
     CDS             8089..>8840
                     /locus_tag="OQS60_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS2388966.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2231 c         2265 g         2223 t
ORIGIN      
        1 tcatttacat cattccctgt ctatgccggt aatattgaat aagaactcta taaattttta
       61 ttcttcgttg gcaccatacg tgtcttgcta catcactgtt caggagcatt taaatattat
      121 ttgtagagct gagtggcaat tatagcagat aatgcttaac tcatcatctt agttcaggtt
      181 tgagcgattt ttgatagtca aaacctcctc tggtgccata cttttctcat aaatattttt
      241 aaaatctata gctcacaaac attatgcaaa gataatgggc tggcatcatt gtcagtatca
      301 ttcatttact gctctgtatg ttatgaatca ttatgttgta tgtttgctct taaaccgtgg
      361 gaaggaggct ataatggttg ccaatattaa tctcattaaa caagaaagtt attctgttgt
      421 taatttagaa aaacaacttt cccgaactgt taccaacaaa attataacga tgagcaagta
      481 aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc accttaaatc
      541 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
      601 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
      661 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gcctcgcaaa
      721 aataaaaaat gatgcgtaaa tgagctttct ttaaagattc ggcaagggta cttttctctc
      781 catagattca aaaatgatat tggcgagata tttatgaact ctctggcagg tatagatatg
      841 ggcagaattt tactcgattt atcgaatgag gtgattaagc aacttgatga tctcgaggtg
      901 cagcgtaatc ttcctcgtgc ggacctatta agggaagcag tagatcaata cctgataaat
      961 caatcgcaaa cagcaagaac cagtgttcct ggcatctggc aagggtgtga ggaagatggt
     1021 gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat gtcaatcaga
     1081 ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
     1141 aaagtcatgc aaattcaata tattgaagtt tattggtagg cctgataagc gcagcgcatc
     1201 aggcaatcat acgcttgtca tcagtgacat acccataacc ttatcgatgt gttggaggga
     1261 agaacgaggc aaacagacaa ttgcggcatg atcttgaatg ggtaacgcgg aatacgagag
     1321 atggtgtcgt cacaccctat gagctgtaat ccgggcgacc tgcgccaccc ggaaaacgtc
     1381 agagattact ctccttcacc cggaaacagg aacgggttaa tggaactacg ggcgtagcct
     1441 tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac ggcttcaact
     1501 ttcggttctt tttcctgata aagaatcttc aggtaagtgc cgcagtcatc gcagctttcg
     1561 gctttaatcg cggcctgttc gtcatccagc gaccagtaat gcagtttgcc gctctgctcg
     1621 cagttgctgc attttacgcg cactacgtgc cattcggttt cacacaggtt gcagtgcagg
     1681 taacgcagac cctgagtggt gccaatttgc accatgctgg acaccggcat agagccacat
     1741 accgggcaat attgacgttg ttcgccgtat tcagcgcggg ctttgccggg gatcagattg
     1801 gccatctgcg cccagtagag cgacagcgca gcccagataa acggcgcttt atcgctgctg
     1861 acggacgaga aatcagaggc aaacagcgcg ctggccatat cttccagctc ctgagtcgat
     1921 gccttctcca gattctcaat cactgccagc gccgggccgc tcatttcagg tttcagctca
     1981 gcaattagcg ccatcagcag cttttgccag tgcttatcac gcggcagaac gtgaatatcc
     2041 agcgggggct tgccctgtgc gctggcttct ttaatgcgcg cggtcagatc catctccagc
     2101 ggatggtcgt acagcaccac ttcctgggcg tgggcgataa gcgcagcaaa gcgcaggtaa
     2161 tcacccagcg gattattttc tgccagctcg cgcagacgct cggcgcggcg gttgtataaa
     2221 ttcttgagcc gagggaacaa taacggcgga atcatatccg ccgtacgttt ctcgctcgaa
     2281 cccagctcat cttgcgggat tatgcgaata ctcattcagc tttcttttcc gttgtcttgc
     2341 ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca ggcgctggta acccatcctt
     2401 ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc accatgataa
     2461 ccacaattaa cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg atcaccggga
     2521 ttgagaaagc aggcgcaaaa taaggacgcc agataatcac gccgctcacc agcagcagaa
     2581 ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc gaagttataa cgcccggtgt
     2641 cacctacttc ctcgttgacg acgatcttac gaatattctt cgcccaaaag atatcatccc
     2701 gattgattag gttgtgatgc cagtaacgga aaaacatgat gatgaacgag gcaaacataa
     2761 ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg atttgcatca
     2821 accagttgaa ggacgggaac aaaaagccca gcccgctcac cgccgccagg atgaagcaga
     2881 aggcggtgat ccagtggttg atacgttccg gcgcggtgta gcgcacgatg gtgtcacgtc
     2941 gtttcatttg cgctcctcgt ctttctcttc gtgcagatta ttctcttcct catccgcacg
     3001 gttcggaccg acacccacgt agtggaagat actggccgca aaggtagccg caaagccaac
     3061 agccgcgagc ggtttccaga tgcctttcca gaatttcacg gtttcgctga tttccgggtt
     3121 ctccggcaag ccatgataca gatttggctt gtcagcatgg tgcagcacgt acatgacgtg
     3181 tgtaccaccg acgcctgccg gatcgtacag atccgcattg tcgtaaccgc gggttttcag
     3241 ctcagcaacg cgctcgctcg ccagcgtttt catcgactct ttcgtaccga agtgaatcgc
     3301 gcccgttggg caggtcttca cgcaggctgg ttcttgccca accaccacgc ggtcaacgca
     3361 cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt cgaacggaca
     3421 gcccgcaatg caataaccgc agccaatgca ctgctcggac tggaagtcga cgataccgtt
     3481 ggcatactga ataattgcac cttccgccgg gcacgctttc aggcagcctg gatcggaaca
     3541 gtgcatacag ccgtctttgc ggatcagcca ttccagtttg tcgttctgct ccacttccga
     3601 gaagcgcatc accgtccacg atttggcgct taaatcattg gggttgtcgt acaccccaat
     3661 gttattgccg acggtatcgc gaatgtcgtt ccactctgaa cacgccacct gacaggcttt
     3721 acagccgata caggtggtga cgtcgatgag tttcgccact tcttcctgga agtcccgcgc
     3781 ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga atgatatctt gcgattgata
     3841 agccatatgt cgtctccgtt acaccttttc cacattcaca aggaaggact taaactccgg
     3901 cgtctgcgtg ttcgcatcac caacgaatgg cgttaacgta ttggcaataa agcctttttt
     3961 agctacgcct tcatagcccc agtgaatcgg aataccgatg gtatcgatat ctttgccgtt
     4021 cgctttcagc gtgcgaatac gtttggtcac caccgctttg gctttgatat agccacggtt
     4081 ggaggagact ttcacggtat cgccctgggc aatgccaagt ttattcgcca gcgactcccc
     4141 gatttccaca aactgctctg gttgcaaaat cgcgttcaac agcgcgtgtt tggtccagta
     4201 gtggaagtgc tcggtcagac gataggtggt tccgacatac gggaacttat cggctttacc
     4261 caatgcttcg gcgtcgtctt taaagattcg cgcagccggg ttcgagataa cgtttggatg
     4321 cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag tgttccggga acggaccttc
     4381 cgtcatctta tcgagggcaa acagacgccc catgccttcc tgctgcatga taaacggccc
     4441 gacgccgctg cccggaggcg ctgcgctgta gtccggaata tcccagccgg tccacttagt
     4501 gccgtcccat ttcagcaact gacgcttcgg atcccacggg ttaccctgcg gatctgcgga
     4561 ggcgcggtta tacagaatgc ggcggttaag cggccatgcc catgcccagc ccagcgtgtt
     4621 accgaggcca gacggatcgg cgttatcacg acgcgccatc tggttgcctt ccggcgtcca
     4681 gctaccggcg aaaatccagc agccacagga ggttgtaccg tcatcgcgca attgggcgaa
     4741 cgagctaagt tgttggcctt tcttgacgat aaccgcaccg gttgccggat cggtaatatc
     4801 ggccagcgcc ttaccgttgc tctccatcgc cacttcttcc gacttcggct catgcggaat
     4861 ggcgtagttc caggtcatgt tcagcacctg atccgggttt gcgccaccct gttcggcata
     4921 catcttgcgc aagcgcagga agataccgga gaggatctcg ccatcagtca gcgcaatccc
     4981 tggggcgtcc gcacctttcc agtgccactg caaccagcga cctgagttaa cgattgaacc
     5041 gttctcttct gcgaagcagg tcgatggcag acggaacact tcggtctgga tcttcgacga
     5101 gtcaacttcg ttcagctcac cgtggttctg ccagaagtta gaggtttcag tgttcagcgg
     5161 gtcgatggtg acgaggaact tcagtttcga cagacaaccg atcactttgt ttttgttcgg
     5221 gaatgaggca acagggttaa agccctggca gatatagcca ttgaccttgc cctctttcat
     5281 catctcgaag tattgcagga cgtcgtagcc tttatcccac ttcggcaacc agtcaaagcc
     5341 ccagctattt tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag agacgaagaa
     5401 tttcgggtag ttgccccagt agtttacctg gccttccagc agcggttttg gcgtgttggc
     5461 ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc agcgtcatgt aacctggcag
     5521 gctctgcgac agcagcccca ggtccgtcag cccctgaata ttggagtgac cgcgcagggc
     5581 gttaacgccg ccgcctgcca tccccatatt gccgagcagc agctggatca tcgccatcgt
     5641 acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg agggcataca ggaacgaggc
     5701 agttttatcg tgagcactgg tttctgcgat gtattcgcag actttcagga acgcgtcttt
     5761 tggcgtacca cagatgtttt caaccacatc tggcgtgtag cgggaaacgt gctgtttcag
     5821 caagttccac acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga agccgttttc
     5881 gtccagttcg taagtccagg aggatttatc gtacttgcgt ttttccgcgt catagccggt
     5941 gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc aggctggcgt tggtataggc
     6001 ttcggtgtat tcgcggttga atttttcatt gttcagcagg tacagcaata cgcctgacag
     6061 gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag tcagccaccg ccgccgtacg
     6121 cgtaaagcga ggatcgatca caatcagctt cgcgccattg tgaattttgg cttccatcgc
     6181 ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg cccatcacca cgacgaggtt
     6241 ggcgttcttg atgtcgaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag
     6301 acttgctacc gttggtccgt gtcagacacg cgcctggttg tcgaccgcga gcatacccag
     6361 cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg aagcacacag
     6421 catcccggtg gagagccagc ggttaacagt cacgccttcg gcgttttgcg caatgtagtt
     6481 agcatcgcgg tcttctttca tcagtttggc gatgcgatca aacgcctctt cccaactgat
     6541 ttgctgccat ttatcagaac ctggcgcacg gtattccgga aacttcagac ggctttcgga
     6601 gtggatgaaa tccaccaggc cagcgccttt cggacaaagt gcaccgcggt tgaccgggtg
     6661 atccggatcg ccttcgatat ggaagataga tgctttggcg ttttttgctc cgtcaccgag
     6721 gctgtacatc aacagcccac agcctacgga acaataggtg caggtattac gggtttcgcg
     6781 ggtgcgcagc agtttatact gccgggtttc cgcgagtgct acgctgggtg caaaacccag
     6841 tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc ttaaagaact gccttctgct
     6901 gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc ccatttacat tcgcttgctg
     6961 cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg gccgcaaagg tccagaattg
     7021 gctcaatttc cctccatcca ggaagggttt gtaacagaat accataatgt tggtgtgtgt
     7081 gttcttatct ggttaagaga aagtgaaaaa aacacagcga aaagaaatcg aaaatgtgac
     7141 aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt gatgatttac aacacccacg
     7201 gttagatgag gtcgcggaag aagttcccgt tgcgctggtc tacaacggca tttcgcatgt
     7261 ggtgatgatg gcgtcgccca aagaccttga gtactttgcg ctcggttttt cgctttccga
     7321 ggggataata gaaagtccgc gcgatatctt cggcatggat gtcgttcctt cctgtaatgg
     7381 tcttgaagta caaattgagc tttccagccg ccgctttatg gggttgaagg agcgccgccg
     7441 ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg gagcaactta atgacatcgg
     7501 aaaaccggtg cagccgctac cgttcaccca gacgtttgat ctcaacaaac tggatgatgc
     7561 attacgtcat ctcaacgatt ttcagccagt ggggcaactg actggttgta ctcacgccgc
     7621 tgcctggatg ttgccatctg gcgaactggt cggcgggcat gaagacgtgg gtcgccatgt
     7681 ggcgctggac aaactgttag gccgccggtc acaagaaggg gaaagctggc agcaaggtgc
     7741 ggtactggtt tccagtcgtg ccagttatga aatggtgcaa aagtcggcga tgtgcggcgt
     7801 agagattttg tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg ctgagcgctg
     7861 taatctgaca ctggtaggtt tttgtaaacc gggtcgggca acggtttata cccatccgca
     7921 gcgtttgagc aattaattaa aataagtcct gcgatatata ttaaattgca ggacttattc
     7981 atttcgtgaa ttttattatt ttatttataa ataaaacgcg tgtagtgacg ttttcttttt
     8041 tcatttatta tgttcactct cttatctata tatttataag gcaattaaat gaaaaggaat
     8101 ttattatctt ccgctattat agtcgccatc atggccctcg gtctgacggg ttgtgatgat
     8161 aaaaaagccg aaacagaaac gcccccgcct gccaatagtc aacctgccgc accagctcct
     8221 gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat
     8281 gaacaagcgg ttttcgacga aaaaatggat gtctatatca agtgctacaa caagttacag
     8341 atccctgtac agaacagtct ggcgcgttat gctgactggc tgaaagattt taaacagggg
     8401 cctaccggta aagagagtac tgtttatggc atctacggca ttagtgaatc caacctcgct
     8461 gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca acctattgat
     8521 ggcgtggcgg tgagttatat tgatgctgcc gtggcgttgg gtaataccat taacgaaatg
     8581 gataaatatt acacccagga aaattataaa gacgatgcgt ttgcgaaagg gaaaacgctg
     8641 caccagacat tcttaaaaaa tctggaatcc tttgaacctg tagcagaatc ttatcatgcg
     8701 gcgattcagg aaattaatga taagcgccag cttgcggaac tgaaaaatat tgaagaaaga
     8761 gaagggaaaa cattccacta ctactctctg gcagtcatga tttcagcgaa acaaattaat
     8821 aacctgattt cgcaagagaa
//
LOCUS       DAJVYM010000146         8810 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVYM010000146 DAJVYM010000000
VERSION     DAJVYM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.148"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..766)
                     /locus_tag="OQS60_003286"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="OQS60_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="OQS60_003287"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="OQS60_003287"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS2388967.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="OQS60_003288"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="OQS60_003288"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS2388968.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OQS60_003289"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OQS60_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS2388969.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OQS60_003290"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OQS60_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS2388970.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="OQS60_003291"
     CDS             complement(4189..4593)
                     /locus_tag="OQS60_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS2388971.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="OQS60_003292"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="OQS60_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS2388972.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="OQS60_003293"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="OQS60_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS2388973.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OQS60_003294"
     CDS             complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OQS60_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS2388974.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2333 a         2187 c         2259 g         2031 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga ctttccgtgt
//
LOCUS       DAJVYM010000147         8633 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVYM010000147 DAJVYM010000000
VERSION     DAJVYM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.149"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OQS60_003295"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OQS60_003295"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS2388975.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OQS60_003296"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OQS60_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS2388976.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="OQS60_003297"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="OQS60_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS2388977.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OQS60_003298"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OQS60_003298"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS2388978.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OQS60_003299"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OQS60_003299"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS2388979.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /locus_tag="OQS60_003300"
     CDS             complement(7825..8136)
                     /locus_tag="OQS60_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2388980.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OQS60_003301"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OQS60_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS2388981.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAJVYM010000148         8547 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVYM010000148 DAJVYM010000000
VERSION     DAJVYM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.150"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..1128
                     /gene="rtcR"
                     /locus_tag="OQS60_003302"
                     /pseudo
     CDS             1..1128
                     /gene="rtcR"
                     /locus_tag="OQS60_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1110..1868)
                     /locus_tag="OQS60_003303"
     CDS             complement(1110..1868)
                     /locus_tag="OQS60_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS2388982.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(1885..2715)
                     /gene="glpG"
                     /locus_tag="OQS60_003304"
     CDS             complement(1885..2715)
                     /gene="glpG"
                     /locus_tag="OQS60_003304"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS2388983.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(2760..3086)
                     /gene="glpE"
                     /locus_tag="OQS60_003305"
     CDS             complement(2760..3086)
                     /gene="glpE"
                     /locus_tag="OQS60_003305"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS2388984.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3276..4781
                     /gene="glpD"
                     /locus_tag="OQS60_003306"
     CDS             3276..4781
                     /gene="glpD"
                     /locus_tag="OQS60_003306"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS2388985.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(4833..5438)
                     /locus_tag="OQS60_003307"
     CDS             complement(4833..5438)
                     /locus_tag="OQS60_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388986.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5441..6208)
                     /locus_tag="OQS60_003308"
     CDS             complement(5441..6208)
                     /locus_tag="OQS60_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388987.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6211..6921)
                     /locus_tag="OQS60_003309"
     CDS             complement(6211..6921)
                     /locus_tag="OQS60_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2388988.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(6937..8442)
                     /locus_tag="OQS60_003310"
     CDS             complement(6937..8442)
                     /locus_tag="OQS60_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS2388989.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2208 a         2208 c         2108 g         2023 t
ORIGIN      
        1 gatctcgatt taagccgtta taacgccatc gccagccgct ttgccgagga acgccagcaa
       61 acgcttgatt ttcttaagtc cggcattgcc acgcgtaacc cccacttcaa ccgcatgatt
      121 gagcagatcg aaaaagtggc gatcaaatcc cgcgcgccga ttctgcttaa cggtccaacc
      181 ggcgcgggca agtcatttct ggcgcgacgc atcttcgagt taaaacaggc gcggcatcag
      241 tttagcggcg cgtttgtgga agtgaactgc gccaccctgc gcggcgatac cgccatgtcg
      301 acgctgtttg gtcatgtaaa aggcgcgttt accggggcgc ggtaatctcg tgaaggttta
      361 ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga ttggcgaact gggcgcagac
      421 gaacaggcaa tgctgctaaa agccattgaa gagaaaacct tttacccgtt tggcagcgat
      481 cgccaggtga gcagtgattt tcagcttatc gccggaacgg tgcgcgattt gcgccagctg
      541 gttgccgaag gcaaatttcg cgaagatctg tacgcgcgga tcaatctctg gaccttcacc
      601 ctgccgggtc tacgccagcg ccaggaagat attgaaccga accttgatta tgaagtggag
      661 cgccacgcca cactcactgg cgacagcgtg cgttttaaca ccgaagcgcg gcgcgcctgg
      721 ttggcttttg cgacctctcc ccaggcaaca tggcgcggta actttcgcga actttctgcc
      781 agcgtcacgc ggatggccac ctttgccact agcggacgca tcactctgga cgtggttgaa
      841 gatgagataa accgtctgcg ctataactgg caggagagcc gcccctccgc gcttacggcg
      901 ttgctgggcg cagaggcaga aaacatcaat ctcttcgacc gtatgcaact ggaacacgtt
      961 atcgctatct gccgctaggc aaagtcgctt tctgcagccg gacgacagct ttttgatgtt
     1021 tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc tacgcaaata cctggcgcgt
     1081 tttggtctga cgtgggaagc cgtgcaggat cagcacagct ccagttgaat atgatggtcc
     1141 gtcagcacct gcattacgct tactggcggc ggggcgtcgg tgtagacggc atctaccatg
     1201 ctgatgctgc ccatattgac catcgcgtta cggccaaatt tcgagtgatc gacaaccagc
     1261 ataacgtggc gcgagttctc aataatggcg cgcttggtgc gaacttcgtg gtaatcgaac
     1321 tccagcagcg agccgtcgct atcgatgccg cttatcccca gaatgccgaa atcaaggcgg
     1381 aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc cgccatcgcg gctgcgtaat
     1441 tcgccaccgg cgagaataat gcggaaatct tcttttacca tcaacgtgtt agcaacattg
     1501 agattgttgg tgacaatacg caaattgctg tgattaagca gcgcgtgcgc caccgcttcc
     1561 ggcgtggtgc cgatatcgat aaacagtgtc gagccgttgg ggatttgctc cgccactttg
     1621 cgggcaatcc tctctttttc ttcggtctgg gtggctttgc gatcgtgcca cggcgtgtta
     1681 accgaactgg aaggcagcgc cgcaccgcca tggtgacgca ggatcaggtt ttgctccgcc
     1741 agctcattga ggtcgcggcg aatagtctgc gggctgacgg agaaatgctc taccagctct
     1801 tcagtactga cataaccctg ctgtttaacc agttcgataa taccgttgtg acgttgtgtt
     1861 tgtttcattt ataaatccct ggaattattt tcgttttcgc gcattgagcg aatcaacaaa
     1921 agccatcgct aaacccacgg ctaacccggc gatgtgtgct ccgttcgcca tcgacatccc
     1981 aaacaaatca aaccatccgg cgacaatcca gatcagcgca aagataatta acccacgttg
     2041 caggtaaatg ccactttgcg gatcgcgttc accacgtagc cagacgtagc ccatcagcgc
     2101 atacaccacg ccagaaagcc cgccaaacca cggcccgctg aatttttgct gcacatagcc
     2161 gcttaacagg gcgctaatga gagtaataac aattagctta ccgctaccga ggcgtttttc
     2221 caccgcaccg ccgagatacc accaccagag caggttaaag aggatatgca tcagcgagaa
     2281 gtgcattaac gcgtgggtga agtaacgcca gaactcaaat ttcagtgctg gatcgaatgg
     2341 ccaggccagc cataacatca cttcctgatc gccgagaatt tgcatggcaa taaacaccac
     2401 cacgcaggcg atcatcatca cccaggtcac cggacctgcg cgttcacgca aggcggcaaa
     2461 gaaaggataa cggcgataat gcaggccact gccggtatgg cctgcctgcc agctggccgc
     2521 cagataacgc ggatctgccg ggttttcgag aaaacgcgcc agctccgccc gtacgcgctc
     2581 ggcctgggac tcatccgcca gccagacatc gctttggtta tgttgttgaa tcgtgaggat
     2641 aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc acgcgggggt tagcaaaaga
     2701 ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa aaggggacag tataaagcgt
     2761 tacgcgccgt acgccacctc tgcgggaaac tgacgttgcc aggcttcaaa gccgccgtca
     2821 atgctataga ccacatcgta gccctgttgc agcagatact gcgccgcgcc tttgctgcta
     2881 ttgccgtgat aacacatcac catcaccgga gtgtcaaagt cgttatcacg cataaacgcg
     2941 cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat gccccatagc gaaactctgt
     3001 ggatcgcgaa tatcgaccag caccgcctct ttttcctgca acttctggtg cgcgtcggca
     3061 acgttaatac attcgaactg atccatgcgt ctctctttct ttacaaacaa gtgggcaaat
     3121 ttaccgcaca gtttacgtcg aagcggcaga taaacgccat aatgttatac atatcactct
     3181 aaaatgtttt ttcaatgtta cctaaagcgc gattctttgc taatatgttc gataacgaac
     3241 atttatgagc tttaacgaaa gtgaatgagg gcagcatgga aaccaaagat ctgattgtga
     3301 taggtggcgg catcaatggt gctggtatcg cggcagacgc cgctggacgc ggtttatccg
     3361 tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc ttccgccagt tcaaaactca
     3421 ttcacggtgg cctgcgctac cttgagcact atgaattccg cctggtcagc gaggcgctgg
     3481 ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc cttcccgatg cgttttcgcc
     3541 tgccacatcg tccgcatctg cgcccggcgt ggatgattcg cattggtctg tttatgtacg
     3601 atcatctggg taaacgcacc agcctgccgg gatcaactgg tttgcgtttt ggcgcaaatt
     3661 cagtgttaaa accggaaatt aagcgcggat tcgaatattc tgactgttgg gtagacgacg
     3721 cccgtctggt actcgccaac gcccagatgg tggtgcgtaa aggcggcgaa gtgcttactc
     3781 ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg gattgtggaa gcggaagata
     3841 tcgataccgg caaaaaatat agctggcaag cgcgcggctt ggttaacgcc accggcccgt
     3901 gggtgaaaca gttcttcgac gacaggatgc atctgccttc gccttatggc attcgcctga
     3961 tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca aaagcaggcc tacattctgc
     4021 aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat ggacgagttt tccatcatcg
     4081 gcaccaccga tgtggaatac aaaggcgatc cgaaagcggt gaagattgaa gagagtgaaa
     4141 tcaattacct gctgaaagtg tataacacgc actttaaaaa gcagttaagc cgtgacgata
     4201 tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga tgagtccgac tcgccgcagg
     4261 ctattacccg tgattacacc cttgatatcc atgatgaaaa cggcaaagca ccgctgctgt
     4321 cggtattcgg cggtaagttg accacctacc gcaaactggc ggaacatgcg ctggaaaaac
     4381 tgacgccgta ttatcagggc attggcccgg catggacgaa agagagcgtg ctgccgggtg
     4441 gtgccattga aggcgaccgc gacgattacg cagctcgtct gcgccgtcgc tatccattcc
     4501 tgactgaatc gctggcgcgt cattacgcac gcacttacgg cagcaacagc gagctgctgc
     4561 tcggcaatgc gggggcgata agcgacctcg gggaggattt cggtcatgag ttctacgaag
     4621 cggaactgaa atacctggtg gatcacgaat gggtccgccg caccgacgat gccctttggc
     4681 gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca atctcgtgtg agtcagtggc
     4741 tggtggaata tacgcagcag aagttgtcgc tggcgtcgta aggatgatgc ccggttgaag
     4801 tgcaaccggg ctatttggat tccttagact attcattgta aataattgaa taacggcccc
     4861 tgagctaaaa tcgccaaact taagcacgtt acccaatcgc cccacttctt cctctggttt
     4921 ctattttttc tgcaccaatg gcaataatat cccaatgcaa aaccaagctg tgcactaatc
     4981 gggccgacaa aatcagtcca aggctgcggc caaaaagggg aaaaattcag gggaggccag
     5041 acaaacgtca gaaagaaatt tcggatattt tccagcaacg ctgatttctc tggagaaata
     5101 taaagaatga aattcgagag aattaaccag aaatagaccg gcgaaaagac cagccagcaa
     5161 tgcagtctgt tttttaattc aggaggcgtt ctgcgggaga attgccaacc gatgccagcc
     5221 attacactga aaaaaagtgc atattcaacc attaactcaa gttgtaattc ctgaagagaa
     5281 atacgcacaa ccaggagcgg aaaggagaat attatttctg ccagaaaagt taacgtgaat
     5341 ataagcacgg ctaagacaat aaccgtcggc cataccaata atggcacgat aacaaaaccg
     5401 agaattacag ggggaatata aagccggaat ttaatcattt ttattttcct ggtgattcgg
     5461 atgatgcgtc atacatcgcg cataaacact catcaattat ccagtcatct tcccagacct
     5521 gatattctgt cattggttcg ggctgtttta gcgggttctc aatcgcttgc cgcgctcttt
     5581 ctttcagtaa aggatgatta tttctagccc aggcattgat ttgggtggta atggcgttat
     5641 cgatatcgtc ctggttatct ctgatataat cctcacaata tctgttcaat aaacaggcaa
     5701 gagaaacggt cgaccaaaag ccgggaactg aattaatggc atccagcaat ggcgcaagag
     5761 cgatatcggc acggaaaaac atttccagca tatactgtaa ttcgacagct tcatcgccgg
     5821 atacccaatc agtcatatac tggcaggcaa atcgctgtaa aattgcccgt tcatccgcac
     5881 gccagttttc tttgttggca aggttgatac ggcgcagaga aaaaactacg tgaatattgt
     5941 caaaatgccc ctgtgcaaca aattccagta aacgcggcag aaaatagcgg acctcatcgc
     6001 tattttgcgg atcaggccct gggctactat tccaggcatt gattaaggaa tacgggatcg
     6061 ctcgtaacga tgtactccgt aatgcctttt gttcctgctg actcaggcaa tattcacaac
     6121 aaaccacaaa tttctgcggc acccgataac gacgaaaggt gtgataaatc tgctcaatta
     6181 atgcctgcat ggcggggctg ggttgcatag ttaccccctc gcccgctgat cgcgtgtaaa
     6241 cggttgatag acaaaacgag cattcgccgc gttcaggcga ataagggcgc tacgcgaaag
     6301 ggaaaagctg gggcgaatgc aactattgca gtctcgacag ctttcctctg cgcaattttc
     6361 cactgagtaa tgacgcgtgt agagtttcag ttcggcctgc ttatcgcgaa atccgcgtaa
     6421 actgttattg ttatgaaatt tccaggcatc tttaaagacc tgaaagatat agacatcctc
     6481 aatccaggag tcacattttt taagcgggcg ctttgctatt atcacccgct catccggatt
     6541 tagcatcgcc gtaaattgta cggtgacgtc atttgtcgtg ttcaggtcac attgaacatc
     6601 tggtacatca ctgatatagg ggtcaaatcc aaaatcagca ccacaggcac aaagacggct
     6661 aatggtggcg gtatcgaggg aaaatcccgg ccataccttg ctggaggtta agccaatatt
     6721 taatgtgaca tcatatcgaa gcaatccctc aagaccgtca gcaccgacga acttttcccc
     6781 ttcatccaag gcctggttaa taagttgctg aacaatgcct tcatcccagt atgccgattt
     6841 gcagttggca atcgtaatat ccacgcctgt gaagggatca gtaccggcaa ggcttgcaaa
     6901 taatgtatta actaattcca tttttattat ccagctttaa ggtagcgtct tatacatcgg
     6961 gacataacct acatcttcca ccaaactctg tccctgcggc gttaaaaacc actccaacaa
     7021 tttttgtgtc tctgacgtcg tattttcccg cgttaccata aaggcatcga cgatataggg
     7081 atatttgccg ttgcgaatgt tttcggcagt cggtgcaata ccgttaatcg ctagcagttt
     7141 tatattttta tcggcgttca tttgtgttgc gtaataacgg aaggtgtaac ctattgcgtt
     7201 gtttgtatta cggtattcgg caacgacttt aatcattccc tccatcatac ttgccacttc
     7261 tgtttcctgc ggcgagatca tgcggacatt tttcatgacc tgggattgca tcaccgtctg
     7321 gctaccagag tcttccgggc gttgccaggt ctggatctcc tgatcgttac cgccaacagt
     7381 acgccagttg gtaattgcac cactgaagat gtcacgcacc tgttgttcag tcagggaatt
     7441 aaccggatta tccgcattga cgatgaaaac aaacgcttca cgggcaaaag gcgtgtacag
     7501 caaagtaacg cccgattcct cagcgcgttt tttctgccca ccggaaggtt gcgccacaaa
     7561 aataatatca gcatcacctt taacaatcct gttatacgca tcgggggtac gagagctctc
     7621 cagatattcc ctggtgtgaa aatcttccgg tataacgctt aaagcataaa atgcagaggc
     7681 ataaattggg tacgccgccg tagcaccatc gagtcgcggc cagttttgcg taaactgaat
     7741 ttgcggttta ccacgcagtg gtgtcagttg attattgagt ttatcgggtc gccaggccca
     7801 ggtatcaata ttttcgcgca caccgacacc gggattgagc tgtttatata aataaccctg
     7861 ataaccagcg acaaaaccaa gcatgagaag gataaacagg agggaattac ggcaacgtag
     7921 cccggatgta ccggttaaag gatgtctggt tggccggccg taccccagcg aaaaactgat
     7981 atgcgcgcca acgggtataa tgactatcca ccagtactca cctccgacta ctgagactaa
     8041 caaaacgccc agccagggga gcataagatc agccaaccaa cccgatccca tctggtgaaa
     8101 ttgataatct gccagatgca gcgcgattat ccagccaacc aggattaata aaccgggtac
     8161 taaaaaaaga cattgtcgcc agacggctat ttctcttttt tctggtgcac tatcgcctgc
     8221 ccatactcgc cccgagaaaa atagcattaa tgcgcccagc gccagcataa aaatgttgag
     8281 cagaaggtaa cttgttggcg tgataaggat attacaaact tcaataatac cggcaagtcc
     8341 gacacccagc atagcaacaa ccgccgccaa aaattgcgtc agtatgggga tgccgaaaaa
     8401 agtcattacc agcgaggaca aaatccattt tctgttttgc attattcttt ccattctttt
     8461 tgaatggtga aattatactc cccgagtccc cttgcccctt ctggacactt ttccgaaatg
     8521 atggcggaaa aaaacgggac cctttgg
//
LOCUS       DAJVYM010000149         8473 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVYM010000149 DAJVYM010000000
VERSION     DAJVYM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.151"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(270..1043)
                     /locus_tag="OQS60_003311"
     CDS             complement(270..1043)
                     /locus_tag="OQS60_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS2388990.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="OQS60_003312"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="OQS60_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCS2388991.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="OQS60_003313"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="OQS60_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCS2388992.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="OQS60_003314"
     CDS             1926..2666
                     /locus_tag="OQS60_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS2388993.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="OQS60_003315"
     CDS             complement(2637..3404)
                     /locus_tag="OQS60_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS2388994.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OQS60_003316"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OQS60_003316"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS2388995.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="OQS60_003317"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="OQS60_003317"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS2388996.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OQS60_003318"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OQS60_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS2388997.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="OQS60_003319"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="OQS60_003319"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS2388998.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8473)
                     /locus_tag="OQS60_003320"
     CDS             complement(8354..>8473)
                     /locus_tag="OQS60_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2388999.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2045 a         2123 c         2229 g         2076 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agc
//
LOCUS       DAJVYM010000150         8287 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVYM010000150 DAJVYM010000000
VERSION     DAJVYM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.152"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            237..758
                     /gene="fecI"
                     /locus_tag="OQS60_003321"
     CDS             237..758
                     /gene="fecI"
                     /locus_tag="OQS60_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS2389000.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            755..1708
                     /gene="fecR"
                     /locus_tag="OQS60_003322"
     CDS             755..1708
                     /gene="fecR"
                     /locus_tag="OQS60_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS2389001.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1795..4119
                     /gene="fecA"
                     /locus_tag="OQS60_003323"
     CDS             1795..4119
                     /gene="fecA"
                     /locus_tag="OQS60_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS2389002.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4164..5066
                     /gene="fecB"
                     /locus_tag="OQS60_003324"
     CDS             4164..5066
                     /gene="fecB"
                     /locus_tag="OQS60_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS2389003.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5063..6061
                     /gene="fecC"
                     /locus_tag="OQS60_003325"
     CDS             5063..6061
                     /gene="fecC"
                     /locus_tag="OQS60_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS2389004.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6058..7014
                     /gene="fecD"
                     /locus_tag="OQS60_003326"
     CDS             6058..7014
                     /gene="fecD"
                     /locus_tag="OQS60_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS2389005.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7015..7782
                     /gene="fecE"
                     /locus_tag="OQS60_003327"
     CDS             7015..7782
                     /gene="fecE"
                     /locus_tag="OQS60_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS2389006.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1801 a         2375 c         2313 g         1798 t
ORIGIN      
        1 gaaatcccta tcctttaggg cagtgaggat gtcaataata gcagaccaca tcatgagtca
       61 ttctgaccga cacatgccag aagcagaggc tccatcccat tttataccta ccttataaca
      121 cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc aattgtaatg
      181 ataactattc tcatattaat atggctacgt gataattaac ttttgatgca ctccgcatgt
      241 ctgaccgcgc cactaccaca gcttccttaa cattcgagtc gctttatggc acacatcacg
      301 gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca gatgacattg
      361 cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc cgcgatcctc
      421 gctccttcct ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc cgaaacgcgc
      481 tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg ccttcacctg
      541 aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg gacgggctaa
      601 acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca tacagcgaga
      661 ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg aaagccgtcg
      721 agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa ccgattcccg
      781 ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtg ctaagcggcg agcgcgtcag
      841 cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata accagtgggc
      901 ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg acgttgccag
      961 ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat tactgctgtt
     1021 gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag gtctgcgggc
     1081 agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg gctccctgct
     1141 cacgctgaat acccaaagcg cggcggatat gcgttttgat gcgcatcagc gcaccgtccg
     1201 gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac gccccttccg
     1261 tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg tccgccagca
     1321 ggataatttc acgcagcttg acgtgcagca gcacgccgtg gaagtgcttc tcgccagtgc
     1381 ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg cctctgagtt
     1441 tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca tcctgagctt
     1501 cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca acggcgtgct
     1561 gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc tgaaaaatac
     1621 cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt ctattacgcg
     1681 gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat tgtccttttt
     1741 acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga aggtatgacg
     1801 ccgttacgcg tttttcgtaa aacaacacct ttggttaacg ccattcgcct gagcctgctg
     1861 ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc accgggatcg
     1921 ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc ggttgacgcc
     1981 agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt cgagagcggc
     2041 ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa taacagctgg
     2101 acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg cgactggctg
     2161 ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt gatccgccgt
     2221 gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg catccctggc
     2281 gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa ctttggcatc
     2341 cggggcctga acccgcgcct cgccagccgc tcgaccgtcc tgatggacgg catccccgtc
     2401 cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct cggcaacatg
     2461 aatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca gagcgtgggc
     2521 ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga ggcgggcgtg
     2581 gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca caacctgatg
     2641 gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg cacgcgcggc
     2701 agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa aagcaaatat
     2761 gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg tgaagccgac
     2821 atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc cacccgcccg
     2881 tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt ccagccagac
     2941 agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag cggctacctg
     3001 gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg tattgagcca
     3061 cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt gggctatcgc
     3121 tatgtgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag cgggcagttg
     3181 ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc gcacgcctgg
     3241 tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat gcgtttcgaa
     3301 catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt gagctataac
     3361 gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa tctttatgca
     3421 aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc tgtgcaaagc
     3481 ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta cgacgacggc
     3541 gcgctgacgg cggaaatggg gctgttcctg attaacttta acaatcagta cgactccaac
     3601 cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct ggaaacgcag
     3661 gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat ctacgccagc
     3721 tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa tctggtacca
     3781 ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa ctggatgttc
     3841 aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac ggtgaaagag
     3901 agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc acgcgtggtg
     3961 tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa aaacatcttc
     4021 gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta tgcaggccag
     4081 ccgcgcacgc tgtatatgca gggggcgttg aagttctgat gtgttaacgc ccggcttgcc
     4141 gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt gcaggcctgc
     4201 tgctggtgat cagccacgcc tttgccgcca cggttcagga cgaacacggc acgtttacac
     4261 tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat gcgctggccg
     4321 ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc atcctgcccg
     4381 aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag ccgagcctgg
     4441 aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc catgcggggg
     4501 tttacatcgc cttgcagcaa atcgcgtcgg tactgctgct taagtcccgc aacgaaacct
     4561 acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa aagcgagaga
     4621 tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc cagcttccca
     4681 aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg catactcagg
     4741 agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct gcgatggcgg
     4801 gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct gcctggctgc
     4861 tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat ccgctctggc
     4921 agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac acctgggcgc
     4981 ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa atcttccacc
     5041 atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc tgtgggggct
     5101 tcccgttgca gcacttatta ttattttctg gctgagtctg ttttgctact cggccattcc
     5161 tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa cgctaccaga
     5221 agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga tcggcgcaag
     5281 cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg cctctccttc
     5341 actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg cgctgagtcc
     5401 gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg gcgtgagctg
     5461 gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa acaaactgat
     5521 cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca ccctgctgct
     5581 ggccgaagat catgcttacg gcatctttta ctggctggct ggcggagtgt cccacgcccg
     5641 ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg tcgtgttgct
     5701 gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata cgctgggagt
     5761 gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg ttggtgcgtg
     5821 cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc tggcgcgctt
     5881 ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg gggccacgct
     5941 gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc tgcccgcagg
     6001 cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga ggcgaggatg
     6061 aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct gttatcactc
     6121 catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca ggccggacgc
     6181 gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc actgtttgtc
     6241 ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa ccctctggca
     6301 tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg ggctctactt
     6361 cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg cggcatggcg
     6421 gggttgatat tgctgaagat gctggcaaag acccaccagc cgatgaagct ggcactcacc
     6481 ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct ctcgcgcccg
     6541 caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg tgactggagc
     6601 tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct gagtttttgc
     6661 cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg tgtgtcggtg
     6721 ccctataccc gattctgggc tttgttacta gctgtcgcca tgacatctac cggcgtggcc
     6781 gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg tagcatcacc
     6841 ggtggacgtc accgcagact gctgcctgtt tcggccctga caggtgcgtt gctgttggtg
     6901 gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt tggcgtgctg
     6961 accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg ataaatgact
     7021 ttacgaactg aaaatctgac ggtcaattac ggaacagaca aggtacttaa cgacgtttca
     7081 ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg cgggaaatcg
     7141 acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt atttctcggc
     7201 gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc gctgctgcct
     7261 cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta tggtcgtaat
     7321 ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt taatgtcgcc
     7381 atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct ttccggcggt
     7441 cagcgccagc gcgcatttct ggcaatggtc ctggcccaga atacgcccgt tgtattactt
     7501 gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg gttgatgggc
     7561 gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa tcaggctagc
     7621 cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca aggcacacca
     7681 gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc ggaaatacac
     7741 cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca ggccgtaaga
     7801 accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag actggggctg
     7861 aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg ctttaggctg
     7921 acaggttcat aatcaacgca aaccagagct gtacaggctt gggcgcggct ttcaaaccag
     7981 tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta taacccttac
     8041 ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat gactaagttt
     8101 agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac attcacggag
     8161 ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag acgccaacgt
     8221 aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc gatttttcta
     8281 aaaagtg
//
LOCUS       DAJVYM010000151         8282 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVYM010000151 DAJVYM010000000
VERSION     DAJVYM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.153"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(277..1113)
                     /locus_tag="OQS60_003328"
     CDS             complement(277..1113)
                     /locus_tag="OQS60_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS2389007.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1113..1916)
                     /locus_tag="OQS60_003329"
                     /note="aph(3'')-Ib"
     CDS             complement(1113..1916)
                     /locus_tag="OQS60_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS2389008.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1977..2792)
                     /gene="sul2"
                     /locus_tag="OQS60_003330"
     CDS             complement(1977..2792)
                     /gene="sul2"
                     /locus_tag="OQS60_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS2389009.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3981..4130
                     /locus_tag="OQS60_003331"
     CDS             3981..4130
                     /locus_tag="OQS60_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389010.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4897..5337)
                     /locus_tag="OQS60_003332"
     CDS             complement(4897..5337)
                     /locus_tag="OQS60_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS2389011.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5555..5944
                     /locus_tag="OQS60_003333"
     CDS             5555..5944
                     /locus_tag="OQS60_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389012.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            5988..6176
                     /locus_tag="OQS60_003334"
                     /pseudo
     CDS             5988..6176
                     /locus_tag="OQS60_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6143..7342)
                     /gene="tet(A)"
                     /locus_tag="OQS60_003335"
     CDS             complement(6143..7342)
                     /gene="tet(A)"
                     /locus_tag="OQS60_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS2389013.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7448..8098
                     /gene="tetR(A)"
                     /locus_tag="OQS60_003336"
     CDS             7448..8098
                     /gene="tetR(A)"
                     /locus_tag="OQS60_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS2389014.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT         1880 a         2281 c         2286 g         1835 t
ORIGIN      
        1 agcgtggact caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg
       61 ggcaccgcag ggaaattctc gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc
      121 ctgcgccctt ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca ggggtcgttt
      181 gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc ggtgagattg
      241 atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct gtcgcacctg
      301 cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg cgttccaagc
      361 tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat cgacgtccag
      421 cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac aaaggtcgtc
      481 tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac cgaccagacc
      541 gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat gatgcagatc
      601 gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta taatcgccgc
      661 gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct gaaacaaagc
      721 tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag atgcggcata
      781 cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac
      841 gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt tctcacgacc
      901 gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc cgcgcagttc
      961 atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa
     1021 tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg
     1081 ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg gtccaatcgc
     1141 agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt gaataggaca
     1201 gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt agcaatcatg
     1261 agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat cagacccgtg
     1321 cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc atggcaaaca
     1381 accatatcgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa
     1441 agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg
     1501 cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaacggacat
     1561 tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg
     1621 agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag
     1681 catgcaccct cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct
     1741 ttgagccaaa tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg
     1801 attttcgcga aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct
     1861 ctgacaggca accagtcaga atgcgattca ccaaaaaaaa tattagttcg attcaatgga
     1921 ggttccttca gttttctgat gaagcgcgaa tatagagaaa tatcccgaat gtgcagttaa
     1981 cgaattcttg cggtttcttt cagcgccgcc aataccgcca gcccgtcgcg caaggggcgc
     2041 ggctcgtgtg tgcggatgaa gtcagctcca cctgcggcgg cggcaagctc tgcagcgagt
     2101 gtcgcggccc cgacatcccc cggaccacgg cctgtgagcg cgcgcagaaa ggatttgcgc
     2161 gaaacagaca gaagcaccgg caaatcgaag cgcagccgca attcatcgaa ccgcgccagc
     2221 accgagagcg aggtttcggg agcagccccc agaaaaaacc ccatgccggg atcaaggaca
     2281 aggcggttgc gtttgatacc ggcacccgtc agcgccgcga tgcgcgcgtc aaagaacgcc
     2341 gcaatgtgat ccatgatgtc gccagcgggt gcctcgcgcc gatctgcctg cccgtcttgc
     2401 accgaatgca taacgacgag tttggcagat gatttcgcca attgcggata gaacgcagcg
     2461 tctggaaaac cgcgaatatc attgagatag gccacaccac gcgacaaggc ataggcttgc
     2521 gtcgcgggtt gataactgtc gagcgagacg ggaatgccat ctgccttgag cgcgtccagc
     2581 accggcgcga tacgcgcgat ttctgtgtcg gacgaaacag gcgcggcgtc gggattgctg
     2641 gatgccggac cgaggtcgat cacatctgcc ccctcggcca tcagcttacg cgcctgcgca
     2701 atggctgcgt ctggcgccag ataccggcct ccatcggaga aactgtccga ggttatgttg
     2761 acgatgccga aaatgatgag cgatttattc atgggggctt ctataataat aataatcgag
     2821 catgagtctc atacggatgc tcgggtcgaa agggaatccc caggcgagta acctgtttgc
     2881 ggtgatccgt ttcatgatga tgtatctgta gcattttgga aataaatata tcatctctca
     2941 ataagtagat atatttatct tttttgcttc tttggtgctg caagtcacca atacgaggga
     3001 aacctgttac ggagcggttt aaccgtaatc aacagtgact ggtgtcatgt tcggaagatt
     3061 aattgatagt aatctacaaa ggatcttctt gagatccttt tcaatgaatc gtaatctctt
     3121 gctctgtaaa cgaaaaaacc gcctggggag gcggtttgtt cgaaggttaa gtcagttggg
     3181 gaactgctta acccggtaac agggttcacg agaccgccat taccaaaact gttctttcag
     3241 tgtagccgca gtcaggcctc tacttcaaga cctcttaatc tatcttctcg tgcactcctg
     3301 ttactaatgg ctgctgccag tggcgttttg tcgtgtcttt ccgggttgga ctcaagttga
     3361 tagttaccgg acaaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     3421 ttggagcgaa ctgccttcac ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat
     3481 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta
     3541 ccagggggaa acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     3601 cgtcagattt tgcgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgtg
     3661 gccttatctc ttctttgcta agtgtcttcc tggtatcttc caggaaattt ccgtccccgt
     3721 ccgtaagccg gtaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     3781 gaagcggaat atctcctgta tcgcatattc tgctgacgcg ccggtgctgc cttttttctc
     3841 ctgccacatg aagcacttca ctgattttcg caatcgtggc aacatagtaa gccagtatac
     3901 actccgctag cgctacatga ctggttcagg gctgcgcccc gaaacccgct aacaacctct
     3961 cagctactgg atatcacccc atgccatctg aatatgatga acaactggat tcgactgata
     4021 cgcagcttcc cggcgaatat gacctttttg atttaatggc gcaggttact gaagagaacc
     4081 tgcatgcccc tgtggatttt gggtcgcctg tcggccggga agcgttatag ctttttcgct
     4141 tgcccgcgta cgtggtaata ctccggtatt accttttatt tctttatttt catgtaatta
     4201 cagtggattt ttaatacccg ggtattactc gtgaaaacga aatattcctt ttttttcttt
     4261 taattcatgc agttgcgatg aaaatcgcgg ctgcgttagg tgtatgacca ttttgaggtt
     4321 gaaaaataat caaccatggc gggtgattta cgcaggttaa ggggagatcg tgacaaaaaa
     4381 agcgttcctc actgtatcgt tcatgcctgc atgaacgggt tattcttcct gactacagaa
     4441 ttacatcgct ccttcagttc atacatcact tttacatcac ttatacatca cttttacatc
     4501 acttatacat cacttttaca tcattccttc agctcatacg tcactttcat cctctgtgcc
     4561 atttgtacat cacccccttc ctgaacgtgc tgacagcggg ttttccggct gcgccggcaa
     4621 cgcttcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc
     4681 cggaaacagg acttaccccg gctgaatggt gtgaatttcc gcgctatgca cttgtgcgca
     4741 tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg
     4801 ttgcctgttt taccggtttc ccgtcagaaa cgccctgagg gcctctcagg cggtgctgat
     4861 aacagatgtt atggtaaatc agagtcacct gcactgttac tctgaaccgg agcgaaacag
     4921 ccgcgaccag aatccccgct ttttttcctg tgccagcgcg tcagccagac ggctaatttc
     4981 ggcctgtagt tgttccctct cctcatttcg caggcggttt tctgcttccc gcgccatctt
     5041 gtcttcaagc attagggtaa cagtctgctg cagagcgtcc agtttctgct gaatgacgtc
     5101 tagctgagca ttttccgcct ggctaacgac agatttggag tgtccctcct tgtgcctttt
     5161 tggtgtctca gaagtgcgca gctcgccata ggcccgaatc agttcactgg tctcgaactc
     5221 ccggcgatca ttttttcctg tgcgatagct gacaaggcct ttagccatgt cacgatataa
     5281 ttgcgagcgc gactttcctg ttaatttcag tgtctcttga actgtgtgcc atgccataaa
     5341 agtgacagtg tctcctgtgt gtaccgtatt gtaacagcag aacatattac tgttttaaca
     5401 caaaaggtct gaggctgccc gtgaggcgtg aaaaatacac aaaaaacctg ttgacggtgt
     5461 ggttttgacg ggcgtagtat ttcgaatact gtatataaac acattgttat aaatacagtt
     5521 tttgctgttt ttaaaagtca aagaggataa ttttatgagt acagagttaa atagcggtac
     5581 tgtcggtggt tttggtcaca tgggaccaaa tgacccctac aatgatggtc atagcggctc
     5641 tggaagttcg ggaggtagtt ttaacggtgg tggtaatggt ggccatgata atagtagtgg
     5701 ttcatcttat actgcatcct ttggtctcat cactggccga cacccgataa ctgtcccagg
     5761 tacgaagaat attaattctg tcgatctttc tgctgtcatt aataacatta tacatcatga
     5821 aggtgcacgc aataacatgt acctagatac tgcgaacaat gtaagagtgg gagtgacggg
     5881 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
     5941 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
     6001 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
     6061 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
     6121 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaaagcc
     6181 cgcgacgcag cgccggcagg cagagcaagt agagggcagc gcctgcaatc catgcccacc
     6241 cgttccacgt tgttatagaa gccgcataga tcgccgtgaa gaggaggggt ccgacgatcg
     6301 aggtcaggct ggtgagcgcc gccagtgagc cttgcagctg cccctgacgt tcctcatcca
     6361 cctgcctgga caacattgct tgcagcgccg gcattccgat gccacccgaa gcaagcagga
     6421 ccatgatcgg gaacgccatc catccccgtg tcgcgaaggc aagcaggatg tagcctgtgc
     6481 cgtcggcaat cattccgagc atgagtgccc gcctttcgcc gagccgggcg gctacagggc
     6541 cggtgatcat tgcctgggcg agtgaatgca gaatgccaaa tgcggcaagc gaaatgccga
     6601 tcgtggtcgc gtcccagtga aagcgatcct cgccgaaaat gacccaaagc gcggccggca
     6661 cctgtccgac aagttgcatg atgaagaaga ccgccatcag ggcggcgacg acggtcatgc
     6721 cccgggccca ccggaacgaa gcgagcgggt tgagagcctc ccggcgtaac ggccggcgtt
     6781 cgcctttgtg cgactccggc aaaaggaaac agcccgtcag gaaattgagg ccgttcaagg
     6841 ctgccgcggc gaagaacgga gcgtgggggg agaaaccgcc catcagccca ccgagcacag
     6901 gtcccgcgac catcccgaac ccgaaacagg cgctcatgaa gccgaagtgc cgcgcgcgct
     6961 catcgccatc agtgatatcg gcaatataag cgccggctac cgccccagtc gccccggtga
     7021 tgccggccac gatccgcccg atatagagaa cccaaaggaa aggcgccgtc gccatgatgg
     7081 cgtagtcgac agcagcgccg gccagcgaga cgagcaagac cggccgccgc ccgaaacgat
     7141 ccgacagcgc gcccagcaca ggtgcgcagg caaattgcat caacgcatac agcgccagca
     7201 gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca
     7261 gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga
     7321 tcaggggtct gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa
     7381 cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt
     7441 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa
     7501 cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca
     7561 gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc
     7621 catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt
     7681 tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg
     7741 catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg
     7801 cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag
     7861 ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg
     7921 cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt
     7981 cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt
     8041 ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc
     8101 aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct
     8161 ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc
     8221 cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcctgc
     8281 tg
//
LOCUS       DAJVYM010000152         8204 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVYM010000152 DAJVYM010000000
VERSION     DAJVYM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.154"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OQS60_003337"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OQS60_003337"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCS2389015.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OQS60_003338"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OQS60_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCS2389016.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OQS60_003339"
     CDS             2028..2546
                     /locus_tag="OQS60_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS2389017.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OQS60_003340"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OQS60_003340"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS2389018.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OQS60_003341"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OQS60_003341"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS2389019.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OQS60_003342"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OQS60_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS2389020.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OQS60_003343"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OQS60_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS2389021.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="OQS60_003344"
     CDS             complement(6710..8170)
                     /locus_tag="OQS60_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS2389022.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2170 a         2055 c         1868 g         2111 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tata
//
LOCUS       DAJVYM010000153         8158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVYM010000153 DAJVYM010000000
VERSION     DAJVYM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.155"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..450)
                     /locus_tag="OQS60_003345"
     CDS             complement(<1..450)
                     /locus_tag="OQS60_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS2389023.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
     gene            complement(567..1613)
                     /gene="ansB"
                     /locus_tag="OQS60_003346"
     CDS             complement(567..1613)
                     /gene="ansB"
                     /locus_tag="OQS60_003346"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS2389024.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1789..2508)
                     /locus_tag="OQS60_003347"
     CDS             complement(1789..2508)
                     /locus_tag="OQS60_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS2389025.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2692..3018)
                     /locus_tag="OQS60_003348"
     CDS             complement(2692..3018)
                     /locus_tag="OQS60_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS2389026.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="OQS60_003349"
     CDS             complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="OQS60_003349"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS2389027.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3898..4950
                     /gene="mutY"
                     /locus_tag="OQS60_003350"
     CDS             3898..4950
                     /gene="mutY"
                     /locus_tag="OQS60_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS2389028.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSCWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4978..5253
                     /locus_tag="OQS60_003351"
     CDS             4978..5253
                     /locus_tag="OQS60_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS2389029.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5318..6397
                     /gene="mltC"
                     /locus_tag="OQS60_003352"
     CDS             5318..6397
                     /gene="mltC"
                     /locus_tag="OQS60_003352"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS2389030.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6599..7855
                     /gene="nupG"
                     /locus_tag="OQS60_003353"
     CDS             6599..7855
                     /gene="nupG"
                     /locus_tag="OQS60_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS2389031.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7904..>8158)
                     /locus_tag="OQS60_003354"
     CDS             complement(7904..>8158)
                     /locus_tag="OQS60_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS2389032.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2160 c         1986 g         2089 t
ORIGIN      
        1 ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga tatcgtcgac
       61 cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagttcaccg accagccaga
      121 cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt accggtttat ctttcgtcca
      181 ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga gatcgtcact
      241 ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat caagattttt
      301 taaggtgata gcatcaagct tgagaatgcc gtgatactgc tttttgaatt gatcttttaa
      361 gatattttca gttggcggtt cgccatctgc ccaggcatta ccagtcatca acataagcag
      421 tgccgtgcca acaatccatt gctttttcac ccgaaaaacc tcacgcggaa taatttattc
      481 cggtgattat cattagggta atgaaaattg gcaatggacg taagtctgaa gtgaaaaagc
      541 cccggcacga taccggggcg aggtgattag tactgattga agatctgctg gatctgctgc
      601 ggatctttgg tttgcgtcag agccagctgc agcaggacgc gcgctttttg cgggttcagc
      661 gtgccagagg cgacgaagcc gtatttcgca tcatccactt cagcatcctg agtggtagca
      721 cccgtcggta cgcgggaaga acgcactact gcagtgccgt ttttcgcggc ggttgccagg
      781 gtgtcgaaca cggatttata caggttacca ttacccacac cagcgctaac gatgccatca
      841 tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta gttataaacg
      901 atgccgactt tcggcagctc attcagctta gagacatcga atggcgtatc gctggtgtgc
      961 ttacgtgccg gggtacgttg gtagtcaatc ttaccgttgt gaatgtatcc cagaggaccg
     1021 tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac atcgcgaccg
     1081 tccagtacgg tgtcgttcat caccaccagc acgccacgat tagcggaggc tttatcagct
     1141 gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcatgga cgtggacggg
     1201 cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc gaggaagtaa
     1261 gcggtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt tttatcgcag
     1321 tcggtgttaa tttttttcgc cagtgtcagc cagacattat cgttcatgtc ctgggagccg
     1381 atattcacta cctgctcgcc tttaacgttc gcaatgtctt ttagttgcgg caccgcatta
     1441 accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc ggagtcacca
     1501 ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc caatgctgca
     1561 ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc catttcattc
     1621 ctccagttac gtgaacgcta cgcattatcc cttagctctg tatgggaaat ttgacgttaa
     1681 acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg taacaaaacg
     1741 aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt attttaaatt
     1801 cccgaccagg gctttgcggc tatcttccag agtcacaacg cggctacaaa catctttgcc
     1861 aaactgctgg aaatcttttt cctgcttttt ccactctgtt tggattgagg attgcagccc
     1921 ccccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca ccgctttcgc
     1981 gcccatttca ttaatgctgt cctgtaaaat tccacccatt gcctgattca ctaattgctg
     2041 gccttcggcg cgaacctgat caatggcttt atagtgaaac gtcaggccat cgctgcgcgt
     2101 ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca gacggctgcg
     2161 cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa tacgggcttt
     2221 ttcgacgcgg cttttcgcgc cttcatcaat ccacggcagc gtgctgcgta gttcagcctg
     2281 ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac cgttatacat
     2341 cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct gcacggtttg
     2401 cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg cgtgagcggt
     2461 cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag tcttccctca
     2521 agaaaaaatc aggccagcat ttgctggccc cagattgata acaaagtacg cgttttccat
     2581 gccggatgcg gcgtaaacac cttatcccgc ctactcgatc gtgcatcttt ccaggcctga
     2641 taagcgtagc gcatcaggca gttttgcatt tgtcatcgcc cgtatgcttt cttagtccca
     2701 ccaaacgtcg aaaagttcgc tggtgcgtac ctcatccagt ttgcgctctt ccagccactt
     2761 acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc agatcagacc
     2821 ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga taacctcgtt
     2881 aataaaatca tcaacggttt tatcaatctg ttcttccgat gtaccttccg ggaatcgcca
     2941 tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac gcagacgacg
     3001 gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac tccgtgacca
     3061 agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg cggcacgtaa
     3121 tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc aagcatatgt
     3181 tccgcataag gttcccagtc ggtcgccata tggaatacac cccccagctg cagtttgctt
     3241 tttaccagtt cggcaaacgg cacctgtacg atacggcgtt tattatggcg cgctttgtgc
     3301 cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat cattttatgc
     3361 agcacttcaa ccgcatcgtg acacatcacg cgcaggttgc ttaaaccttc ttcatgcgca
     3421 gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa gtcctgctca
     3481 gggcgatctt tagccattgc caccagcgac gcccccatgc caaaaccaat ctcaagcgtc
     3541 accggcgctt cacggccaaa aagcgcgggg aaatccagca tatcttcgct gaactcaacg
     3601 cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag tcgcccctgg
     3661 cggcgcacaa aactacggat acggcgcagt gggcggccgt tttcatcaaa ttccggtgaa
     3721 atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa acgggcatta
     3781 tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa gttccggttt
     3841 acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc ggtgaccatg
     3901 caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg gcgaaaaacg
     3961 ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt gatgttgcaa
     4021 caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg cttcccgacg
     4081 gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac cgggcttggc
     4141 tattacgccc gcgcgcgcaa tctgcataaa gcggcacaac aagtggcgac cttacacggc
     4201 ggtaaattcc cggaaacctt tgaagaagtc gcggcgttac cgggcgtcgg gcgttccacc
     4261 gcaggcgcga ttctctcgct ttctctgggt aagcactttc cgattctcga cggtaacgtc
     4321 aaacgcgtgc tggcgcgctg ctatgctgta agctgctggc ctgggaaaaa agaggtcgag
     4381 aataaattat ggagtttgag cgagcaggtg acgcccgcgg tcggcgtgga acggtttaat
     4441 caggcgatga tggatttggg cgcgatgatt tgtacgcgct cgaagccgaa atgttcgctc
     4501 tgtccgctac aaaacggatg tattgccgcc gccaataata gctggtcgct ttatccgggc
     4561 aaaaaaacga aacagacgct gccggagcgc accggctact ttttgctgtt acagcacgaa
     4621 gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcggttt atactgtttc
     4681 ccgcagtttg ccgacgaaga aagtttgcgg cagtggctgg cgcaacggca gattgctgcc
     4741 gataacctga cgcaactgac cgcgtttcgg cataccttca gccatttcca cttagatatt
     4801 gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg caatgcgctc
     4861 tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga gcgtttgtta
     4921 cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg atgatttatg
     4981 agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga ttttcagctg
     5041 taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc ctgggcgcag
     5101 tggcagcaca agcaaaccat gctgattaat gaaaagaaac tcaacatgat gaatgccgag
     5161 caccgcaagc tgcttgagca ggagatggtc aacttcctgt tcgagggtaa agaggtgcat
     5221 atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac gcctccggca
     5281 acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc
     5341 tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga
     5401 agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg cccacaatat
     5461 tgagaacatc tggggattca aagaggtggt gatcgccggt cctaaggact acgtgaaata
     5521 caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga
     5581 aactatcgcc gggacagaac ctgccgcgca tttgcgccgg gcaattatca aaacgttgct
     5641 gatgggtgac gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc
     5701 gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga
     5761 aggtcgcgca agcaacttcg cggattatct gctgaaaaac cgtctgcaaa gccgcagtaa
     5821 cgggctgcgt atcatctaca gcgtcaccat taacatggta ccgaaccacc ttgataaacg
     5881 tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggcg ttgatgagtc
     5941 gctgattctg gcaattatgc agaccgaatc ttcctttaac ccgtatgcgg tcagccgttc
     6001 cgatgcgctg ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc
     6061 gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga
     6121 taccggcacc gcgtatctgg cgatgctgaa caatgtttat ctcgacggaa ttgataaccc
     6181 aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg
     6241 agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga cgccgggcga
     6301 tgtttatcag acgctgacga cccgccatcc ctctgcggaa tctcgccgtt atctttataa
     6361 agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cattaaccgc ccctgacgat
     6421 gctcaggggc aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc
     6481 aattatttgc cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc ccatcatctc
     6541 gcacggtcaa atgtgctttt tcaaacactc atccgcatca caatgtgagg aaattaacat
     6601 gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg
     6661 gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg
     6721 cgcagtttat agctcactgg gtatcgccgc ggtatttatg cctgcgctgc tggggattgt
     6781 ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat
     6841 cacgctgttc atggcggcac aggtcacgac gccggaagcg atgttccttg tgatattgat
     6901 taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct
     6961 gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat
     7021 cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca
     7081 gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca
     7141 tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc
     7201 attcgcgctg tttaaaaaca agcgtatggc aatcttcttc atcttctcaa tgctgctggg
     7261 cgcggaactg cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga
     7321 tccgatgttt gccagcagct tcatcgtgca gcatgcgtca atcatcatgt cgatttcgca
     7381 gatctctgaa accctgttca ttctgaccat cccgttcttc ttaagccgct acggtattaa
     7441 gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg
     7501 cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt acggttgcgc
     7561 attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat
     7621 tcgcgccagt gcacaaggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg
     7681 cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta ccgactggca
     7741 gaccgtatgg ttgattttcg ctggttattc cgtggttctg gccttcgcgt tcatggcgat
     7801 gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg
     7861 caaagaaaaa cgggtcgcca gaaggtgacc cgttttttta ttcttacttc aacacataac
     7921 cgtacaatcg tttcatgcca tccgcatcgg tttcgctata gacaccttgc aactccggcg
     7981 aaaatcccgg caacaaattc acgccttctt ccagcgcaag gaaataacgt tgaaccgccc
     8041 caccccagac ttccccggga accacgcaaa gcacgccagg tggataaggc aacgcccctt
     8101 ctgccgcaat tcgcccttcg gcatcacgaa tccgcaccaa ctccacttca ccgcgaat
//
LOCUS       DAJVYM010000154         8120 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVYM010000154 DAJVYM010000000
VERSION     DAJVYM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.156"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..326)
                     /locus_tag="OQS60_003355"
     CDS             complement(<1..326)
                     /locus_tag="OQS60_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389033.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALL"
     gene            433..1326
                     /locus_tag="OQS60_003356"
     CDS             433..1326
                     /locus_tag="OQS60_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2389034.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            1461..2681
                     /gene="mlc"
                     /locus_tag="OQS60_003357"
                     /gene_synonym="dgsA"
     CDS             1461..2681
                     /gene="mlc"
                     /locus_tag="OQS60_003357"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS2389035.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2806..3501
                     /gene="bioD"
                     /locus_tag="OQS60_003358"
     CDS             2806..3501
                     /gene="bioD"
                     /locus_tag="OQS60_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS2389036.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3454..4746)
                     /gene="clcB"
                     /locus_tag="OQS60_003359"
     CDS             complement(3454..4746)
                     /gene="clcB"
                     /locus_tag="OQS60_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS2389037.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4905..5519)
                     /gene="dmsD"
                     /locus_tag="OQS60_003360"
     CDS             complement(4905..5519)
                     /gene="dmsD"
                     /locus_tag="OQS60_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS2389038.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5562..6416)
                     /locus_tag="OQS60_003361"
     CDS             complement(5562..6416)
                     /locus_tag="OQS60_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS2389039.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(6418..7035)
                     /gene="dmsB"
                     /locus_tag="OQS60_003362"
     CDS             complement(6418..7035)
                     /gene="dmsB"
                     /locus_tag="OQS60_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS2389040.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(7046..>8120)
                     /locus_tag="OQS60_003363"
     CDS             complement(7046..>8120)
                     /locus_tag="OQS60_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS2389041.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2067 a         2058 c         2123 g         1872 t
ORIGIN      
        1 agtagcgccg tgaccatcac tggtttgcga ccaatggcat cggatagggg gccagtaaac
       61 agcaaaccaa tagccaacat cgccgtggaa atggacagtg aaatactact gttcgcgggg
      121 gttaagccaa actcctgcga aagcaccgga aggataggct gcacacaata gaggagtgca
      181 aaggttgcca gtccggcaga gaacagcgcc agggtgacgc gcataaattg cggcgtaccg
      241 cgtttaataa attgatttgg ctgagaaatg ctttgcttgt cagtgtcgct tgccggagcg
      301 ccatcaacag ttgtagtacg gctcacttga aatccttgct aaatatgcct gtggatcagg
      361 cttatacata ggttaggaaa atcgaattgt tctgtctaat atattaataa tctcaaataa
      421 gatgttttaa atatgaatat tgaacttcgt catctgcgtt actttgttgc tgttgcggaa
      481 gagctgcatt tcgggcgcgc cgctgcccgc ctgaatattt cgcaaccacc gctaagtcag
      541 cagattcagg cactggagca acaaattggt gcccgactgc tggcacgaac taatcgcagt
      601 gtattgctga cggcagcagg aaaacagttt cttgcagata gtcggcaaat cctgtctatg
      661 gtagatgacg ctgccgctcg cgctgaaaga ctgcatcagg gtgaagcggg ggagttgcgc
      721 attggtttta cttcgtccgc tccttttatt cgggcggtgt ccgatacgtt atcgctgttt
      781 cgccgtgatt atcccgatgt ccatttacaa acccgcgaaa tgaacactcg tgagcaaatc
      841 gctccgctca ttgaaggaac gctggatatg ggattgctgc gtaacacagc gttaccggag
      901 acgcttgaac acgcagtcat cgtccatgaa ccgcttatgg cgatgatccc gcacgatcat
      961 cccctggcaa ataacccgaa tgtaacgctg gctgaactgg cgaaagaacc cttcgtcttt
     1021 tttgatccgc acgtcgggac agggctgtat gacgatattc tcgggctgat gcgacgttac
     1081 catttgacgc ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt
     1141 tccgccggtc tgggtgtttc aattttgcct gcttcattta aacgtgttca gctcaacgaa
     1201 atgcgctggg tgccgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg
     1261 aaacatcatg aacaaagtcc agcttcgcgt aactttcgta ttcatctgct gaatgctctc
     1321 aggtgaggga aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt
     1381 aaaaatttga cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc
     1441 gaaaatatag ggagtatgcg gtggttgctg aaaaccagct tgggcacatt gatcaaataa
     1501 agcagaccaa cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta
     1561 tcgatctttc ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga
     1621 tgctcgaagc acacctggtg caagagctgg aaatcaaaga agcggggaac cgtggccgtc
     1681 cggcggtggg gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc
     1741 gcggggagat tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc
     1801 aggaactggc gttaaaagat gacttgccat tgctggatcg tatcatttcc catatcgatc
     1861 agttttttat ccgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc
     1921 cgggaattat tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa
     1981 aagagatgcc gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc
     2041 atgatatcag cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg
     2101 atgtgattca ggtggttatc gatcacaacg tgggggcggg cgtcattacc gatggtcatc
     2161 tgctacacgc aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccatatg
     2221 gtaaacgctg ttattgcggg aaccacggct gcctcgaaac catcgccagt gtggacagta
     2281 ttcttgagct ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catgggcaac
     2341 cgttaaccgt ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca
     2401 tcattaccgg ggtgggcgcg catgtcgggc gcattcttgc catcatggtg aatttattta
     2461 acccacaaaa aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttccctg
     2521 tcatctcgga cagcatacgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg
     2581 agagtactca gttttctaac cagggcacga tggcaggcgc tgcgctggta aaagacgcga
     2641 tgtataacgg ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac
     2701 caaaatttgc gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat
     2761 tattttccta gtttatattt gtgaagcata acggtggagt tagtgatgct ggagcgtttc
     2821 tttattaccg gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa
     2881 gcgttagcct cccagggaaa aacagttgcg ggatataaac ccgtagcgaa ggggagcaaa
     2941 gagacacccg aagggctgcg taataaagat gccctggtgt tgcagagtgt ttcaaccatc
     3001 gaactgcctt atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg
     3061 cacagttgcc caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa
     3121 gtcgatcatg tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt
     3181 ccactctctg aatgggtggt gcaggaacag ttgccggtgt tgatggttgt cggtattcag
     3241 gaaggttgca ttaaccatgc actgctaaca gctcaggcga ttgccaacga cgggctgccg
     3301 ctcattggct gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaagtcatt
     3361 gatgtgctgg gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc
     3421 gccgaacagc gagaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg
     3481 gtagatagag tcacggtgta acgtccgcga aattaccgac gcaatgacgc aggcaatcaa
     3541 taaaccgggg agtagctgat actccccggt catttcacat atcatcaacg tcgacataat
     3601 cggcgcgtgc gtggttgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt
     3661 aatttcttcg ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc
     3721 aagaccgata aatagcgtcg gtgtaaagac accaccgggt gcgccggaac cgctactcgc
     3781 cagcacggca cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc
     3841 ggttaaaaag gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga
     3901 aagcagaccc acgatcaacc cgcctagtgc cagttgccag ggcggcgcaa gtttgagact
     3961 cacaaatcca cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc
     4021 cagcacacct gtactgataa tcagcgcata gtcactagcc tgaaccgtta ctgagagttg
     4081 tacgctgtag agtaacgcgt cgctatgatt aataagattg ctaaccagca acgccacgac
     4141 ggcggaaata atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc
     4201 tataaataaa ctgccagcaa gcggggcgcg ataggccgca gccattcccg ccgcggcccc
     4261 acaggcgatc cataatttcc attcctggcg tggcgtaaaa cgttgggcaa aacaggaggc
     4321 ggcaagggca gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac
     4381 taccagcagc gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt
     4441 ttgcaacgct tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca
     4501 gcccatcagc aacaaacccg ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca
     4561 gggggaaagg tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca
     4621 ctccagtagc agcatcgcat gacgaaaccc ggcaacggca aaggccgcga gaataccgac
     4681 gactgttgcg ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg
     4741 atgcatgagc agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccaa
     4801 ccgcaaaagc agtgcgtgct ttaagagata aaaaaacgtg acattgtaac ttattattgc
     4861 cgaatcgcgc gtaatcgtca ccatccggca atattacggt gatcctatcg aaagagtggt
     4921 ttaaccgcga caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt
     4981 tcacccagtg cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt
     5041 gttgaccacg gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc
     5101 tccgccaacc acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc
     5161 ttcatttcaa actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg
     5221 ccaaacagca cagattcgcg atccagccag accgatcccc acggcggaga cggcagtgcc
     5281 cacgggccga caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga
     5341 aaagccgtca ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg
     5401 tcactggtga gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac
     5461 agcgcgccca acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt
     5521 cctaacatcc tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt
     5581 cctactgtca tgtgcaggcc ataaaagagc acacgcccga tcatctcacc aacaagtatg
     5641 aggattagcc ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa
     5701 agccagcatc caagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa
     5761 tctggtacca gtgcgctggc ttgttgcacg gatgagtgaa tagacgccag agacaaacct
     5821 tgcagcacaa tcaccccggc acaagcgatc agcgcgagaa cgctaatgat ggcaaatggt
     5881 gtggtattaa atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc
     5941 aacaataccg tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacagtg
     6001 tcgatttgat acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca
     6061 agtaaccaga gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat
     6121 agtccgccaa cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct
     6181 ccgattcgat tcagcgagtt aaacgcccgc agtggcgatc ccaggtgcat gacagaggcc
     6241 ataaacccga tgcccatcaa cagccagagg aaaaacatgc cacggacaat acgctgacgg
     6301 tctgcgtcat ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg
     6361 cattgaccca atacggtaaa gataaccagt ggccactcat gccatccatt tcccatttta
     6421 cacttcctcc ggattagcca gataaccagt ggtatcgctg gtcgggcggc tgttggcgtt
     6481 aggtttgata acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc
     6541 cagagtgccg tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg
     6601 gcaggattcg acacatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca
     6661 cttcgtcatg tgcccttttt cagcattgta ctgtggcgcg ccgtacggac aagccatgtg
     6721 gcagtagcgg cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt
     6781 atgcattgcg ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga
     6841 gatggagaga taataagcga acacgttctg gtgccagacg ccattatctt cctgccagtc
     6901 gccgccagca tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc
     6961 tttgcacgcc agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc
     7021 atattgtgtg gtcacgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg
     7081 acgggtttcc ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc
     7141 cgtgatcgac ccggtcacca aacatatccg ctttaagcca agcaccttgc ccgatggcgg
     7201 taacgccagg cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt
     7261 taaatacccg cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atggggttga
     7321 tccacacctc ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg
     7381 tacgcgcttt gtaatggaag cccgttaact gcagtggata ggttttccgc aggggatcgt
     7441 cccagccatc aaaacctggg gtatacgcag gaaggggatg aataacttca tcttttttca
     7501 attcccaggt atccgcaatc ttcgccagtc gttcagaata aatttcgatt ttccccgaag
     7561 gtgttttcaa cgggtttgcc tgtggatctt cacggaatgc gcggaaagcg acgtagtgtt
     7621 cttccgggca ttttttctta aagatcccgg tcgttttcat ctcctcgtag tcgggcatct
     7681 cagggttacg ttccttcgtt ttcgcatgga gatatttgat ccattcatgc tgactgcgac
     7741 cttcagtaaa ggtttgataa acgtctggtc ctaagcgttt ggcgacttca ctcagcatcc
     7801 agtagatggg tttgcgttca aattttgctg aggttgcggg ttgggcgagg atcacatagc
     7861 ccatattccc tgcagattca tgagagataa ggtcttcttg ctctgttggc atcaggtcgg
     7921 gcaacaggat atcgcaatac ttagccgagg ccgtcatgaa gtggtcaatg ccaacaatca
     7981 tctcgcactt gctgtcatcc tgaagcacct catgggtgtg attgatgtcg ccatgttgat
     8041 tgatcaatgt gttactggcg tagcaccata aaaacttgat ggggacatcc agtttttctt
     8101 ttccacgaac accatcacgg
//
LOCUS       DAJVYM010000155         8093 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVYM010000155 DAJVYM010000000
VERSION     DAJVYM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.157"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            297..1568
                     /locus_tag="OQS60_003364"
     CDS             297..1568
                     /locus_tag="OQS60_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS2389042.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1598..2602)
                     /gene="dppF"
                     /locus_tag="OQS60_003365"
     CDS             complement(1598..2602)
                     /gene="dppF"
                     /locus_tag="OQS60_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS2389043.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2599..3582)
                     /gene="dppD"
                     /locus_tag="OQS60_003366"
     CDS             complement(2599..3582)
                     /gene="dppD"
                     /locus_tag="OQS60_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS2389044.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3593..4495)
                     /gene="dppC"
                     /locus_tag="OQS60_003367"
     CDS             complement(3593..4495)
                     /gene="dppC"
                     /locus_tag="OQS60_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS2389045.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4505..5524)
                     /gene="dppB"
                     /locus_tag="OQS60_003368"
     CDS             complement(4505..5524)
                     /gene="dppB"
                     /locus_tag="OQS60_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS2389046.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5832..7439)
                     /gene="dppA"
                     /locus_tag="OQS60_003369"
     CDS             complement(5832..7439)
                     /gene="dppA"
                     /locus_tag="OQS60_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS2389047.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7791..>8093)
                     /locus_tag="OQS60_003370"
     CDS             complement(7791..>8093)
                     /locus_tag="OQS60_003370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389048.1"
                     /translation="VGLIRRASVASGNGTAFIVGCGATALFDQHTTHFPANFAPHISL
                     SINGFVTETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1869 a         2189 c         2049 g         1986 t
ORIGIN      
        1 cggcttgccg ctgattttac gattgctttt tactgtcagc acccggcaat cttcctcatt
       61 gcgcgatgcc ttccagctta atttctttat ttgcaatagt tgcagttcat atccgggctg
      121 actccagact ggtagtgaaa tgcgcaattt tgtagaattg cgcctcctcg cgacctcccc
      181 agtgaatctg ttgcctcatt ttttacatac tgcgtattcg acttctccac ctgttgcgca
      241 agagaaactg ggtttattca tttttgcgag gccgacttct ttctggacag gactttatgc
      301 agcacaacac actatcgaaa cacaatcaga aattgccgtt tacacgctac gacttcggct
      361 gggttttatt atgcataggc atggcgattg gtgccggaac cgtgctgatg ccagtacaaa
      421 ttggcttgaa gggaatttgg gtatttatta ccgcagcgat cattgcttat cctgccacct
      481 gggtagtgca ggacatttat ttaaaaaccc tttctgaaag cgattcctgt aatgactaca
      541 ccgatattat cagtcattac ctggggaaga actggggaat tttcctcggg gttatctact
      601 ttttgatgat tatccacggg atttttatct actctctctc cgtggttttc gacagcgcct
      661 cgtacctgaa aaccttcggt ttaaccgatg ccgatctttc acaatctcta ctttataaag
      721 tcgctatttt cgccgtactg gtagcgattg cgtctggtgg tgaacgatta ctgtttaaga
      781 tttccgggcc aatggtggtg gtcaaagtag ggattattgt cgtgttcggt tttgcgatga
      841 tcccgcactg gaatttcgcc aatataaccg ccttcccgca agcctccgtc tttttccgcg
      901 atgtcttgct taccattcca ttttgcttct tttctgcagt atttattcag gtacttaacc
      961 caatgaatat tgcctatcgt aaacgggaag cggataaagt actggcaacc cggctcgcgc
     1021 tgcgtaccca ccgaattagt tatatcacgc tcatcgcggt gatcctgttt tttgcctttt
     1081 cgtttacctt ctcaattagc cacgaagaag ccgtttctgc ctttgaacaa aatatctcag
     1141 cactggcgct ggccgcgcag gtgatccctg ggcatatcat tcatatcacc tctacggtgc
     1201 ttaatatctt tgccgtactg accgcattct ttggcattta tctcggtttc cacgaggcca
     1261 ttaaaggcat tattctcaat ctgttaagcc gaattattga taccaagaaa attaactcac
     1321 gcgtgctgac tctggcgatc tgcgctttta tcgtcattac gttgacgatt tgggtttcgt
     1381 ttcgtgtatc ggtgctggtg ttctttcagt tgggaagccc gttatatggt attgtgtcgt
     1441 gcctcattcc gtttttcctg atctataaag tcgcacaact ggaaaaactt cgcggattta
     1501 aagcctggct gattctgctg tacggcattt tgctatgctt gtcgccactg ttgaagctga
     1561 ttgagtaaac cggagcgcat ggccccggtt ttgtgagtta acgctgcgga ttttcatcct
     1621 gatcaacagc aaaacaagct accagttgac cgccgtagtc ttttagctgc ggctgcaact
     1681 gggtgcaggg gccgaagcgc cgacgacagc gggcgttgaa ggcgcaaccc ggcggtggat
     1741 tcagtgggct tggtagttca ccgctgagct tgatgcgctc gcggcgatcg tccgggttca
     1801 ggcgcggcgt cgcggaaagt agcgcctgag tgtacggatg gcgcgggtta ttgaagattt
     1861 ggtctttcgt tcccttctcc acgcagcggc ccaggtacat caccatcact tcatcagcaa
     1921 tgtgctccac caccgacagg tcgtgggaga taaagacata agacagcccc aactcctgct
     1981 gcaaatccat catcagattc agcacctgcg cgcgcactga aacatccagc gcggaaaccg
     2041 gttcatcggc aatcaccaca tccgggtcga gcatcagacc acgggcgatg gcgatacgct
     2101 gacgctgacc gccggagaac atatgcggat agcggtcata gtgctcggtt ttcaggccga
     2161 ctttcgccat catcgacagg gctttttccc gacgctgttc tttgcttaag ctggtgttga
     2221 tcagcagcgg ctcttcaaga atttgcccga cttttttacg cggattcagc gaaccgtaag
     2281 ggttctggaa gacgatctgg attttctgcc gacgcagctt ctgcgcctgc ggatcgtgct
     2341 taagcagatc ctgcccctgg taatacagct cgccaccggt gggcgtttca atcatcgtca
     2401 gcaaccgacc gagggtcgat ttaccgcagc cagattcgcc cactactgcc agcgttttgc
     2461 cacgttcaag gttaaacgaa acgccatcca gcgctttaac cagacgttcc ggcgcgaaca
     2521 tgcctttctt caccggataa tgttttttca ggtcgatagc ctgcaacagc ggttgttgca
     2581 gggtggcctc ttgcgtactc atagtgtcgg cctcccggca tcatcaagtg ggtaatggca
     2641 tttggactgg cgcccgtcag cgagcatatt cagcgccggt tcttcagcgc gacatctgtc
     2701 agtggcatag gggcagcgcg ggttaagcag gcagccgttc gggcggtcgt acttgccggg
     2761 aacgacacct ggcaacgacg ccagacgttc tttgtcctga gcaaattctg gcagcgcacg
     2821 cagcaatgcc tgagtatacg ggtgacgcgg cgcatggaag atggcgtgcg catcgcccgt
     2881 ttccaccacc tggcctgcat acatcacaat gattttatgc gcagcttccg ccaccagcgc
     2941 cagatcatgg gtaattaaca ccagcgccat gttctctttc tgctgtagct ccagcaacag
     3001 ttcgatgatt tgcgcctgaa tggtcacgtc cagcgcagtg gtcggctcat cggcaatcag
     3061 cagttttggc cgacaggcaa tcgccatggc gatcatcacg cgctggctca tgccgccgga
     3121 aagctgatgc gggtaaacat ccagacgcga tgccggatcg ggaataccga cctgattcag
     3181 caggtcaatc gcccgctgac ggcgagtgct cttattaccg ccctgatgca ccttaatcgc
     3241 ttccatgatc tggaaaccca cggtgtagca cgggttaagg ctggtcatcg ggtcctggaa
     3301 gatcatcgcc acttcggcac ccaccaggtt gcggcgctct ttttctgata tacgctgcaa
     3361 atcctgaccg ttaaacacca gcttttccgc cattacgcgg cccggataat caatcagccc
     3421 cataatcgcc agtgaactga ccgacttacc ggagccagac tcacccacaa tcccgaccac
     3481 ttcgccctgt tttacgctgt agctgatgcg gtctacggcg cggaacggtg cgctttcgtc
     3541 gccgaaatgc accgataatt tatctacatt taataacgcc atctcgaacc tcttactgct
     3601 tcagtttggg atcgagcgcg tcacgcagac cgtcacccat caggttaaat gccagcaccg
     3661 tcagcaggat cgccagaccc gggaaggtca cgacccacca ggcgctttgc gcgaactgca
     3721 acacgtcgga gagcatggtg ccccactctg gtgttggcgg ctgcgctccc atgccgagga
     3781 agccaagggc ggccatatcg agaatggcgt tagagaaacc gagcgacgcc tgaacaatca
     3841 gcggcgccag gcagttcggg aagatgttaa taaacatctg acgcatcgcc ccggcacctg
     3901 ccacgcgaga cgcggtaacg taatcgcgat tcacctccac cagcacggcg gcgcgggtta
     3961 agcgcacata gtgcggcaag gcaacgaagg tcagtgccag cgcggcgtta ccaatcgacg
     4021 ggccgaaaat cgccaccagc accagtgcca acagcagact tggcagcgcc agcatgatat
     4081 cgaccacgcg catgatgatg ttatcgacca ggccgccgaa gtaaccggcg atcaggccga
     4141 gaataacgcc cataatcagc gataacacca ccaccagaca gccaaccagc agcgacaagc
     4201 gcgcgccgta catcagccgc gacagcacat cacggcctac gtcatcggtg cccagcaagt
     4261 gcgccatgct gccgccttcc tgccaggctg gcggggcgag cagcgcatcg cggaactgat
     4321 ccgccgggtt ataaggcgca atccagttgg caaagatcgt gataaacagc acgatgacga
     4381 tgtaaaccag cccgacaacc gcgcctttgt tgcgtttaaa atagtgccag aactcctgta
     4441 acggggtcat cggcaccggt gcgctaatca ctttattttc agtaacctgt gacatgatgt
     4501 ccccttactt cttatgacga atacgcgggt tcaccacgcc gtacagcaga tcgaccagca
     4561 ggttgacgag gataatcatc gtcgccacca gcaatacgcc gccctgcact accggatagt
     4621 cgcggcgttg cagtgcgtca atcaaccagc gtcccagacc gggccacgag aagatggttt
     4681 cggtcagaat cgcccccgcc agcaatgttc ccacctgcag gccgataacg gtcactaccg
     4741 gcagcatcgc gttacgcagc gcatggacga taatcacccg catgcgggtc aaccctttgg
     4801 cgcgcgcggt gcggatgtaa tcttcaccca gcacttccag catcgaagag cgcgtcatac
     4861 gaacgataac cgccagcggg atagtaccca gcacaatagc cggtaagatc atatgggcaa
     4921 cggcgtcgat aaagttgcca tcttcacccc agatggcggt gtcgatcagc ataaaaccgg
     4981 ttaacggatt ggagtcatcg aggaacacca tatcgctcac gcgaccggag acgggcgtca
     5041 ggttccagtg caccgaaacc agcatgatca gcatcatgcc ccaccagaag atcggcatcg
     5101 agtagccggt cagcgccagg ccaactgcag tgtgatcgaa aatggaaccg cgtttaaccg
     5161 cagccagcac accaaccgga ataccgactg ccgtagcaaa aatcatcgcg cagacgccaa
     5221 gttccagcgt ggcctgaaag cgcggcacga actcttccca tactggaatt cggcttttca
     5281 ttgaaatgcc tagatcgccg tgcattacgc cccaaatgta atggagatac tgctgccaca
     5341 tcggtttatc taagccgagt tcagccagca gctgcgcgtg acgctcaggg gagatcccgc
     5401 gttcgcctgc catgatcatc accggatcgc ccgggatcat gtggacaaag gcaaatgtga
     5461 gaagggtaat accgataaac gtggggatga cgagtcccaa acgtcggaga ataaactgca
     5521 acataacccg gattctctat agtgacccgc agcctggaac tgcgtgtctg tattgctcac
     5581 aaatcaattc ccgcccttat cggacaggga atgaagtatt gctgccggat ggcgctgcac
     5641 gcagaggcac atcgccaccg ggtatacaga ttgttgacaa agtgcgcttt gtttatgccg
     5701 gatgcggcgt taacgcctta tccggcctac aaaatcgtgc aaattcaata cattgcagaa
     5761 ttttcgtagg cctgataagc ggagcacatc aggcagtttt gcctttgcca tcagtcttgt
     5821 atggctttta attattcgat agagacgttt tcgaagtgat gtttgcctaa tggatcaacc
     5881 acatagcctt tgacttcttt gcgtaccggt tcaaacacgg tggagtgagc gatgatcagt
     5941 gccggagtct gatcgtgcat caccacctgc gcttgcttgt acagttcaac gcgtttgtta
     6001 tggtcgtcgg tagcacgcgc cggttgaatc agatcttcaa acggtttgta gcaccatttt
     6061 gagtagttag aaccttgttc agaggcggcg cagctgaaca gggtggcgaa gaagttatcc
     6121 gggtccccgt tatcgccagt ccagcccatc attaccgtct gatgttcgcc atctttcgca
     6181 cgcttgaggt actcacccca ttcgtaggtg acgattttgg cctgcacgcc cactttcgcc
     6241 cagtctgcct gaatcatctc cgccatgcgg cgagcgttcg ggttatacgg acgttgtacc
     6301 ggcatcgccc acaggtcgat ggagaaacct ttttccagac ccgcttcttt cagcaaggct
     6361 ttcgcttttt caggatcgta ggtgtaatcc tgaacgtcgt cgttatagcc ccacatggtt
     6421 ggcgggatca ggtttttcgc tgatacaccc gcgccctgat aaaccgcttt gatgatcgcg
     6481 tctttgttca ccgcgtaggt cagtgcctgg cgaactttca cgtcatccag cggttttttc
     6541 tgcacgttat aggagaggta gccgacgttc agccccggca tttccatcag gttgatggat
     6601 ttatcctgct tcatgcgagc gatgtctgcc gggttcgggt acggcatcac ctggcattca
     6661 ttcttctgca atttcgcgta acgcacggaa gcatcagggg taatagagaa aaccagcgta
     6721 tcgatctgcg gtttggtgcc ccagtagcca tcaaacgctt tgtagcggat acgggaatct
     6781 ttttggtact gctgtaactg gaacggaccg gttccgattg ggttgaggtc cagtttttcc
     6841 ggtgtaccgg ctttcatcat cgcatcagca tattcttttg acagaataga ggcgaagtcc
     6901 attgccagat cagcgaggaa cggcgcttcc gggcgagtca gtacgaactg aactgtgttg
     6961 tcgtccacct ttttcacttc actgatcagc tccggcaagc ccatgccttc gaagtattcg
     7021 tagctgccgc cagagacttt atggtacggg ttttgcgcgt ttttctgacg gtcgaacgag
     7081 aacaccacgt catcggcgtt cagttcacgc gtcggtttga attctttatt gtcgtgccac
     7141 ttcacacctt tacgcagatg gaaggtatag gttttaccgt cttcgctgac ttcccacttt
     7201 tcagcgaggc ccgggatcac ttcagtggtg ccgattttaa attcaaccag gcggttataa
     7261 agcggtacgg aagaggcgtc ataggtggtg ccggaggtaa acagctgcgg gttaaaccct
     7321 tccggagatc cttctgagca ataaaccaga gttttagcct gaacacttgc tgcgacggtc
     7381 atagccacca ggctgagacc aagcttcagc atccctgact ttttcaagga aatacgcatt
     7441 attctgctcc aattgtgatg tttgttgttt taaccctttg cagtgggttg tcgctgcctg
     7501 accttttttg ttttttgccc ggtcgggtca acgttatgag gtggggatgc cgtactaatt
     7561 aacatcagtg taacagtgcg gatcctccat aaaatgcccc tcgtgaccta caatctgtca
     7621 acagaatgtg aaaacgtcaa tacagccaac cgggatttac accaacggtg agaatccaca
     7681 aacaaagatt aaaaaaactt caaacagcta tttgcagcag agaaatttgt gctactccaa
     7741 cataaccaga acaatcagct taatatttag caacatttgg tgattaagtt ttatgcattt
     7801 aatgaaaaaa atctgaggaa aaggtggata tctgagcgat taataccatg aacgctaaaa
     7861 cgcacagcgg aaaatgccag cgccagccat aagtaacgca aaaaaagatt taagcaaata
     7921 taaaaaaaga caatggtttc tgtcacaaat ccgttaatgg atagtgagat atggggcgca
     7981 aagttggcag gaaaatgtgt ggtgtgttgg tcgaataagg cggtcgcgcc gcatccgaca
     8041 ataaatgcgg tgccgttgcc tgatgcgacg ctggcgcgtc ttatcaggcc tac
//
LOCUS       DAJVYM010000156         8068 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVYM010000156 DAJVYM010000000
VERSION     DAJVYM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.158"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..278
                     /locus_tag="OQS60_003371"
     CDS             <1..278
                     /locus_tag="OQS60_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS2389049.1"
                     /translation="SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTR
                     FGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
     gene            582..827
                     /locus_tag="OQS60_003372"
     CDS             582..827
                     /locus_tag="OQS60_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389050.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            824..1246
                     /locus_tag="OQS60_003373"
     CDS             824..1246
                     /locus_tag="OQS60_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS2389051.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1906..3773
                     /locus_tag="OQS60_003374"
                     /pseudo
     CDS             1906..3773
                     /locus_tag="OQS60_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            4032..4313
                     /locus_tag="OQS60_003375"
     CDS             4032..4313
                     /locus_tag="OQS60_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389052.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4953..5273
                     /gene="clpS"
                     /locus_tag="OQS60_003376"
     CDS             4953..5273
                     /gene="clpS"
                     /locus_tag="OQS60_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS2389053.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5304..7580
                     /gene="clpA"
                     /locus_tag="OQS60_003377"
     CDS             5304..7580
                     /gene="clpA"
                     /locus_tag="OQS60_003377"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS2389054.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7986..8067)
                     /locus_tag="OQS60_003378"
     tRNA            complement(7986..8067)
                     /locus_tag="OQS60_003378"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8037..8039),aa:Ser,seq:gga)
BASE COUNT         2216 a         1773 c         2201 g         1878 t
ORIGIN      
        1 cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga aattattccg ttcagtccgc
       61 gcaggtatct ttatcacgcc tatctggcat atatgagggc acacggattc ggtaaacctg
      121 taacactgac gcgcttcggt aaagatatgc cgggggcaat ggcggaatat ggcagggagt
      181 atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt gaccctgaca gaggattcag
      241 aagactggat gccatcatgt gcaataggtc acaaatgacg atggaaaaaa ttaaacttat
      301 ggaataactg ttcaccactg ttcaccctgc aataaatgtc ttttatatca gtatattata
      361 gggtgaacag ttatttatga actgttcacc aaactattca ctgttcacct ttttgattct
      421 ttattgagct ttaagggtga acagtggtga acagttggtg aatagttttt gtgaaactgt
      481 taacccatta acatcatgaa taaaaaggga aaattgcaaa aggtgaacag gtgaagggtt
      541 aaaatgcaaa aattttattt tattgctgtg aggtaaagcc tgtgacaacg aagcacgcca
      601 aaaaaccaca atctcacgcc cttgatttga cagaacactg gctgagggtg gcgataaaaa
      661 tcatcgaccg caacgccggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat
      721 tcatgaccac gacggcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg
      781 aacaggtgac aaccatcaac gttaaaaccg gagagcagac agcatgacag cacagatagc
      841 ggcttacggg cggctggtgg ctgacccgca gttaaagacc accagcaagg gtacgcaaat
      901 gacgatggcg agtatggcgg ttcccctgcc gtgcagccag gcagatgacg gaacggcgac
      961 gatatggtta tccgtcctgg cgtttggcag acaggctgac gcactggcaa agcaccagaa
     1021 aggcgagctg gtgagcgtgg cgggtaacat gcaggtaagc cagtggacag gccagaacgg
     1081 agaaacgcgg cagggctggc aggttatcgc agacagtgta atcagtgcca gaacggcgcg
     1141 accgggcggt aaaaaaggcc aacaggggca ggccactgat gcactgaaca gggccaaaca
     1201 acaggcgggg aatgatgatc cgtacgggga taacataccg ttttaagcaa cgagtgacag
     1261 aagccggagc aatccggctt ttttgtaggt actcctggtg ggggtggcct gtccacgggg
     1321 cgggcggttc gcggaaaaag gcgtattttt tgatttttat gtcaccatca ccaccagttt
     1381 aagttattga tatatggcaa aataaaaatt tttagtgtcg aattaggttg ttttttgttc
     1441 gacgccggaa cgttcccgaa agcatttaca aaaaacaggc gcaaaaaaaa gcgccccgat
     1501 ggtgccaccg gaggcgcttt gtcagcgaca aaggatactg ccactaccga aatgatgagt
     1561 cttaattgtg ctggcgtggc aataattagt caattctttt gctggtatga caaaaatcag
     1621 aaaacacata atctgatttt tagatagaaa atgatttatt taccagtttt atcgatcaat
     1681 aatgatgccc gttaatcata acggaggcag acttatgcca gagaacaaca gcagaaaacc
     1741 ggataaaagt gcaacagtgc acatagacgc cggaactatg gagaagctcg aacgctatca
     1801 gcagttcatt aaaagtcatc accctggaat gccagtaccc acgaaagggc agattgcgag
     1861 aagcgcggtt gaatactggt acagggcaac gttaggagcc tggctatgaa aacatggttt
     1921 tccattaagg ctatggctga tgttgtacat gtacgcattt atgacgagat cggcgggtac
     1981 ggtgtaaaag catcggcact tactgacgag atcaacgcgt gcggtaatgt gcctgaaatc
     2041 catcttcgca tccattcacc tggtggcgac atctttgaag ggctggctat ctataacgcc
     2101 ctgaaaaatc atccggcaaa gaaaattgta cacattgaag gcatggcggc ttctatggcc
     2161 tcgtttattg ccatgtgtgg cgatcacatc gttatgcctg aaaacgcgat gatgatgata
     2221 catgcccccc gtggtgttac tgccggagtg tcgggcgacg ttcgccgctt tgctgacctg
     2281 atggacaagc tgggcgacac gatggcggaa acctacgccg gaagaacggg caggagcaaa
     2341 caggaaatca ccgccatgat ggaggcggaa acctggatgg atggcaatga gtgtaaggct
     2401 aatggcttcg cagatgaggt tatacccgcg attacagcaa tggccagaat tgaatcaaaa
     2461 cgaatcggag atttttcgaa tatgccggaa aatattaaaa gcatgatcag ccagaaaact
     2521 ggcagcggag aacaggaacg actcaacggg atacgtgaat tgtttggcac gttcaatgga
     2581 agatataacg acctggctat aagttgtctt gctgattcag gctgtagcgt tgaggatgca
     2641 cgcgaacgcc ttttactcac tatgggtaaa gaatcgacac cgacaaacaa aaccaccccc
     2701 gcaaatcttt actacgcgta cacggataac ggcaacataa ccggcgatgc aatgcgtcag
     2761 gggcttaatg cgcgtctggg acatgaacag gcagaacgcg gcaaccctta cgccatgatg
     2821 agccttttcg atatggcaca ggcatcatta acccatcgcg gcataagcac aggcagctac
     2881 agcacacgct cgcagatagt aaacgcggca ttcacccaca gcagcagcga ttttacttat
     2941 atccttgctg gtggtgctga aaaatccgtg aacacagcgg cgaaacgttc cgccagtgga
     3001 cgaaaaaagg ttcattgtca aacttccatg aggcacgtcg tgttggcctg aatggcttct
     3061 ctacattaaa caaagtgccg gaaggcgcgg aatataaata catcaccacc agtgaccgtg
     3121 gcgaaccaat cgcgctggca acttacggaa atattttcag cattacccga caggcgatta
     3181 ttaacgatga cctgaatcag ttaacagtta tcccacaggc aatggggcga gcagccgcca
     3241 gaacggtggg taatctggtt catctggtgc tgacaggaaa cacaaagctt tctgacggta
     3301 taccgctgtt cagcaaaaaa cacggaaatg atattgataa cggagtcagc actacagggc
     3361 ttggcattgc acgccgtgca atgcgcacac agagggatga aaacggtgag gtgctgaata
     3421 ttgtgccaaa attccttctt gttcctgcgg ctctggaaga cagagcgttg caaatggtta
     3481 actcaaccac gcctttcggt gccgatacaa acagtggttc atataacccg taccacaaac
     3541 aactggatat tattgtcgat ccccgccttg atgaagtcag tgataagcaa tggtacatgc
     3601 tttcagcaca gggaacggac acaattgagg tggcttatct tgacggtaat gacgagcctt
     3661 accttgagca gcaggagggc tttattgttg acggtgtggc ctggaaggtc cgcattgatg
     3721 ctggcgttgc tgctctggat tatcgcggaa tggtcagagc gatttcttca tgagaacaag
     3781 gcggcagcag ccgccttttt tgcgggtcct cctggtgggg ggggcctgtc cacggggcgg
     3841 gtcggcgcgg aaaaaagcgc atttttgtgg ttttatcgtc atcatcatca tgtgtgtaac
     3901 ctgttgtttt taatgtagtt gatgcaaaaa agatgatgat tgtggttaat ttttgttcga
     3961 catcttttag cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg
     4021 atgggggcac aatgacagaa gccgaaatac tgggattaat ccgccgcgtc gccggaatca
     4081 gccagcaggt tgacgaacag gccatgcaac cggacagcgt gaccgctgaa aattatgcgc
     4141 gtgtggtgga tgaggtaatg cgccgtgacg gtattgagct taacggcgtg gatatgcgca
     4201 acatacgaac cagagttctt gagttgctgg cataccgtcg ccgttctcaa caacggaggg
     4261 agagcgcgaa aaatacttac cagtggagga agccggagca cttacggcgg taactggttg
     4321 atattaccga aagtgcaaaa atgcacttag caactggtgg cgagttgcag atctgcaact
     4381 cgactatgaa actacggaaa ctacccgtag tttgggtagt aagagtaaca cccagatttt
     4441 ggggcttact cgcgataccc aaataaaggg tatcggtgga agaaatatcg tttctcatat
     4501 gtgagtaccg agggcggaat tccgccttcg gttacttatt gtgcgcatgc acagggaggg
     4561 gcgggtcaaa tctctgtacc ctgacgtctt ccggactgcc agccccatcg attttttata
     4621 cccgcgaaaa atgaaaaacg cttcacgctg gtgggcctga tgccgataac tggtttcatg
     4681 actgatagcc ggaaaatgat aacgattaaa gcgtgttgat ctttgttcgt ggttgttcga
     4741 tattgttcgt ggaacggtgt agttaatggt gtagttaatt ttactgtttt tgacgagctt
     4801 attgtttgca actcaatgaa aaataaaggc taataagcca aatgctataa atgatagtta
     4861 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
     4921 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
     4981 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
     5041 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
     5101 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
     5161 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
     5221 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
     5281 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
     5341 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     5401 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     5461 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     5521 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     5581 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     5641 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     5701 atgtggtgaa ttttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     5761 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     5821 cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga
     5881 aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     5941 tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc
     6001 agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     6061 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     6121 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     6181 cgggggcagc gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca
     6241 gcggtaaaat tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     6301 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcaatcgaag
     6361 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     6421 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatc aatgatcgtc
     6481 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     6541 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     6601 ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg
     6661 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     6721 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     6781 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     6841 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     6901 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     6961 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccggacg
     7021 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     7081 aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg
     7141 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     7201 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc
     7261 tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     7321 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     7381 aaggttacga ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga
     7441 aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     7501 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     7561 aggcggaagc agcgcattaa tctaattgtt aggtaggttg gtgaagtccg taatctcgaa
     7621 agaggttacg gactttttgt ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     7681 aggtaatttg ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca
     7741 ggaaaatgag atggttttgc tttattaaca acggactaaa cgtgtagtat ttgagttcac
     7801 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
     7861 tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac
     7921 tgccgtacag aaagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
     7981 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
     8041 ggcgtgctcc ttcagccact cggacacc
//
LOCUS       DAJVYM010000157         8053 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVYM010000157 DAJVYM010000000
VERSION     DAJVYM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.159"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            154..2172
                     /gene="fadH"
                     /locus_tag="OQS60_003379"
     CDS             154..2172
                     /gene="fadH"
                     /locus_tag="OQS60_003379"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS2389055.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(2217..2633)
                     /gene="higA"
                     /locus_tag="OQS60_003380"
     CDS             complement(2217..2633)
                     /gene="higA"
                     /locus_tag="OQS60_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS2389056.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(2630..2944)
                     /gene="higB"
                     /locus_tag="OQS60_003381"
     CDS             complement(2630..2944)
                     /gene="higB"
                     /locus_tag="OQS60_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS2389057.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(3229..4365)
                     /gene="rlmG"
                     /locus_tag="OQS60_003382"
     CDS             complement(3229..4365)
                     /gene="rlmG"
                     /locus_tag="OQS60_003382"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS2389058.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            4450..4953
                     /locus_tag="OQS60_003383"
     CDS             4450..4953
                     /locus_tag="OQS60_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS2389059.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            5030..5713
                     /locus_tag="OQS60_003384"
     CDS             5030..5713
                     /locus_tag="OQS60_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS2389060.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            5792..6778
                     /locus_tag="OQS60_003385"
     CDS             5792..6778
                     /locus_tag="OQS60_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2389061.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            7061..8026
                     /gene="alx"
                     /locus_tag="OQS60_003386"
     CDS             7061..8026
                     /gene="alx"
                     /locus_tag="OQS60_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS2389062.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         1866 a         2066 c         2160 g         1961 t
ORIGIN      
        1 acacggcaag cgtcgcatcc ggcgttacca ccgggcgtat tctttttgaa tcccatcaca
       61 aaccccgcac tccccttttc ccttttctcc atcgacggct aaattagaac tcatccgacc
      121 acataacaat tattttacat actggacaat tttatgagct atccgtcgct gttcgccccg
      181 ctggatttag gttttaccac gttaaaaaac cgcgtgttga tgggctcaat gcacaccggg
      241 ctggaggaat acccggacgg tgccgagcgg ctggcagcgt tttatgccga acgcgcccgt
      301 cacggcgtgg cgctgattgt cagcggcggc attgcaccag atttaacagg cgttggcatg
      361 gaaggtggcg caatgctcaa cgacgccagc cagatcccac accatcgcac cattaccgaa
      421 gcggtacatc aggaaggcgg caaaatagcc ctgcaaattt tgcataccgg gcgctacagc
      481 taccaaccgc atctggtcgc tccctccgca ttgcaggccc ccatcaaccg tttcgttcct
      541 cacgaactca cccatgaaga gatcctgcaa ctgatcgacg atttcgccca ctgcgcgcaa
      601 ctggcacggg aggcaggata cgacggtgta gaggtaatgg ggtccgaagg gtatttgatc
      661 aacgaatttc tgacgctgcg caccaatcag cgtagtgacc agtggggcgg cgattaccgc
      721 aaccggatgc gatttgccgt agaagtagtg cgtgcggtgc gcgaacgcgt cggcaacgac
      781 ttcattatta tctaccgact gtcgatgctc gacctggtcg aagacggcgg gacttttgcc
      841 gaaacggtag agctggcgca ggccattgaa gcggcgggcg cgaccattat caacaccggc
      901 attggctggc atgaagcacg tattccgacc attgccacgc ccgtgccgcg cggcgcattt
      961 agctgggtca cgcgcaaatt gaaaggccac gtctcgctgc cgctggtaac caccaaccgg
     1021 attaacgatc cgcaggttgc cgacgatatt ctctcgcgcg gcgatgccga tatggtatcg
     1081 atggcacgac cgtttcttgc tgatgcggaa ctgctgtcaa aagcgcaatc ggggcgagcc
     1141 gatgagatca acacctgcat tggctgcaat caggcctgcc tcgatcagat cttcgttggc
     1201 aaagtcacct cgtgcctggt gaatcctcgc gcctgccacg aaaccaaaat gccaatcctt
     1261 cctgccgtgc agaaaaaaaa tctggcggtg gtcggtgcgg gacctgctgg gctggcgttt
     1321 gccattaacg cggcggcgcg tgggcatcag gtaacattgt ttgatgccca cagcgagatc
     1381 ggcgggcagt ttaatatcgc caaacagatc cccggcaaag aggagtttta cgaaacgctg
     1441 cgctattacc accggatgat cgaagtgacg ggcgtgacgc tgaaactcaa tcacaccgtg
     1501 acggcggatc agttacaggc gttcgatgaa acgatcctcg ccagtgggat cgtgccgcgc
     1561 gttccgccta tcgacgggat cgatcatccg aaggtattga gttatctcga tgtactgcgc
     1621 gacaaagcgc cggtaggcaa caaggttgcc atcatcggtt gtggcgggat tggttttgat
     1681 acggcgatgt atttaagtca gccgggcgaa tccaccagcc agaatatcgc caggttctgt
     1741 aatgagtggg ggatcgacag cagcctacaa caggctggtg gcttaagccc gcagggaatg
     1801 cagatcccgc gtagcccacg gcaaattgtg atgctccagc gcaaagccag caaaccggga
     1861 caggggttag gcaaaaccac cggctggatc catcgcacca ccctgctctc gcggggtgtg
     1921 aaaatgatcc caggcgtaag ttatcagaag attgacgatg acgggctgca tgtggtgatc
     1981 aacggcgaaa cgcaggtatt agcagtggac aatgtggtga tctgcgcagg gcaagagcca
     2041 aaccgcgcgc tggcgcaacc gctgattgat agcgggaaaa ctgtgcattt aattggcggc
     2101 tgcgatgtgg ctatggagct ggacgcacga cgggcaattg cccagggaac acggctggcg
     2161 ctggagattt aaatcgttat tttaagcgcc ggatgcaacg catccggcac gtcgcgttaa
     2221 tcaataaaca aggcgggaga aatgccgaat cgcgttgcca attttttagc gtgttccagc
     2281 gttaatttcc tcttcccgct caaaacgcgt gacaccatag atttactgcc aatttccggc
     2341 agatcggcaa gggttaaacc atattgatcc ataagggtac gaatcacggc tataccgcca
     2401 ggcatggctt gagccatggc attaaattcc gcaaattcgg gcgctgattc ttcccacgcg
     2461 gttattttgg cacacaccag atccagcaag gggttttcag gatcgttgag cagcagatga
     2521 tctaccagtt ccagcgcctg ggtgtattgt tcctcgttct gaatacccgc cagaaaaggt
     2581 gccaccgcag ttagcttttc tcctgcttgc aagatgtcgg caatcgcaat cattttttcc
     2641 ccttagtacg atgaacagcg gtaaagaaat cgtattcttt atgcgtcata acttcacgta
     2701 tgtagcactt ttgcgattca aaaaagacca ttgctacaac acgtaattca ttgcccccaa
     2761 cattgaaaac ataatgctta tccagatatt tgaagttatc cagagatggg aatactgctt
     2821 ttaatgactc aggttttttg aaatatccct tagcaatcgt gttccccaga gccaccaact
     2881 ccgttttatg ttgcgggtat ttttccgcag catctttcaa tgctttttga gttatcaggt
     2941 gcattcttca tcacgtccgt tgacaaattg gcaatatgat aacatccgtt gacagattgg
     3001 caacggatga atttatctgt gtgacggaag aataaacgta gaaaaacgct aaacaagaga
     3061 atgtagagga tggtgagagg gattttttgt tttatggaag cgcgattcca gaactgggca
     3121 gacattgccg gatgcgacgc ttgccgcgtc ttatccggcc tgggagtaac gtgctgaacg
     3181 taggccggat aaggcgttaa cgccgcatcc ggcaggtaac tcccaatctt accgacgacg
     3241 ccccagcttc actgctttca gcaccacaaa tttattattc gtggcgatgg tggtgcagtt
     3301 gccgaaaatc ttcttcagtt tatggaagta atccaggtga cggttggcaa caatatacag
     3361 ctcgccgttg attttcaggc agcggcgggc atggtggaac atctcccagg cgacgttatc
     3421 ggtcagcgca tgttgttggt gaaacggcgg gttgcagagc acagcattaa agcggaaagg
     3481 ctccacgccg gagagcgcgt tgttgatcat aaactcgctg cgatctaacg cctctggcat
     3541 gttggtttca acgttcaaac ggctggaagc aaccgccatc ggcgattcat cgacaaacac
     3601 cactttcgcc tgagggtttt tatcaagcag cgtcagacca ataacgccat taccgcaacc
     3661 gagatcgaca atctcacctt cgagattctc tggcagatgt tgcataaaga agcgtgcgcc
     3721 aatatccagc ccggtgcggg agaagacatt cgcatggttg tggatagtcc agtcagtacc
     3781 ttccagcttc cagctaacgg tctgcggcgc atcggccagc ggcggttcat tgaaagtgca
     3841 attaatcagg cgcgcttttt tccatgccag cgtagtggtg gtcgggccga gcactttctc
     3901 gaacagctcc agcgtggaag tgtgaatgtc acgggctttg gcaccggcaa taatacgtgt
     3961 atccgacgtg accactttgc gcagcgcacg cagttgctgt tccagcaatg ccagtgtttt
     4021 cggcactttg atcagcacca cacccggctg ttgcgggtag tcggcggtgc tgtcgagaaa
     4081 cttcacgctc gactcatcaa tcccgttgag gcgtaaattc tcgcgcgtcg ccagttcact
     4141 gatgtatgag tcgccaatgc tgtacggctt atgttccgcc agcgcgcagc ttaacgcacc
     4201 aaaggcatca ttcaggatca acaccgggcc gcggatttct gtgtcgtcca actgttgcag
     4261 caaatattca tccgccgctt cccacgcctg tagcgggtta acgtcatccg tcgccggaaa
     4321 acgttgtagt gtcagtgaac ggaaaccgtt gtctaagtgg ctcatcggcc ctcctgaatg
     4381 ataaaatttc gacgttgccc ctgaaaaggg tgcgtgagta tacccttttt ctctttttcg
     4441 tgggcggtta tgagcaatct tacttatctt cagggttatc ccgagcagct actttcccag
     4501 gtgcgaacgc tgattaacga acagcgtctg ggcgatgtgc tggcaaaacg ctatccggga
     4561 acacacgact acgccaccga taaagccctc tggcaatata cccaggatct aaaaaatcag
     4621 tttctgcgca atgccccgcc gatcactaaa gtgatgtatg acaataagat ccacgtactg
     4681 aaaaacgcgc tcggattaca taccgctgtt tctcgtgtgc agggcggcaa gctgaaagca
     4741 aaagcagaga tccgcgtcgc tactgtgttt cgcaacgcgc cggaaccgtt tttacgcatg
     4801 atcgtggtgc acgaactggc gcacctgaag gagaaagagc ataacaaagc gttttaccag
     4861 ttgtgctgcc atatggagcc gcagtaccac cagcttgagt tcgacacccg gctgtggcta
     4921 acgcagttgt cgcttgggca ggacaaaatc tgaaaatgat gtcaggcgtt atcttttctt
     4981 tctttagcgc ctaaaatcga cctcccccct ttcgttaagg ataacgacca tgcagccccg
     5041 ccttcttctc cgtatttgtt tttctcgccg gactctaaag attacctgct tactgttact
     5101 tgtggcagga gcgaccattt ttatcgccga tcgcgtaatg gtcaacgcca gcaagcagct
     5161 gacctggagc gatgtcaacg cagttccggc gcgtaacgtg ggtttattgc tgggggcgag
     5221 gccgggtaat cgctacttta cccgacgtat tgataccgca gcagcgttgt accacgtcgg
     5281 aaaagtgaaa tggctgttag tcagtggcga taacggacgt aaaaattacg atgaagcctc
     5341 aggtatgcag caggctttga tcgctaaagg cgtaccggcg aaagtgatct tctgcgacta
     5401 tgccggattc tcaacgcttg attcagtggt gcgtgccaaa aaggtgtttg gggaaaacca
     5461 tatcaccatt atctcgcaag aatttcataa ccaacgcgcc atctggctgg caaaacagta
     5521 cggcatcgac gctatcgggt ttaacgcgcc agaccttaat atgaagcacg gtttttatac
     5581 tcaactgcgg gagaaactcg ctcgtgtcag cgcggtgatt gacgccaaaa tcctccatcg
     5641 ccagccgaaa tatctcgggc catcggtcat gattgggccg tttagtgagc atggctgtcc
     5701 ggcgaaagaa taatgcgtat ctgcgcacgt cgaagatgaa aaaggcgtgc tacattgacg
     5761 acagaatccc tttatggagt atccacgcgt tatgatacgt ttcgctgtga ttggtacgaa
     5821 ctggatcact cgccagttcg tcgaggccgc ccatgagagc ggtaaataca agttaaccgc
     5881 cgtatattcc cgcagccttg aacaggccca gcacttcgcc aatgattttt ctgtcgagca
     5941 tctgtttacc tcgctggaag cgatggcgga aagcgatgcc attgacgcgg tgtatattgc
     6001 cagcccgaat gccctgcact tttcccagac acaacttttc cttagccata aaattcatgt
     6061 gatttgcgag aaaccactgg cgtcgaatct ggcggaagtg gatgccgcca ttgcctgtgc
     6121 gcgggaaaat caggtggtgc tgtttgaggc atttaaaacc gccagcctgc cgaactttca
     6181 tttgctgcgc caggcgctgc cgaaagtcgg caaattgcgt aaagtctttt tcaactattg
     6241 ccagtattcc tcgcggtatc aacgttacct ggatggtgag aatcccaaca cctttaatcc
     6301 ggcattctct aacggttcaa ttatggatat cggcttttac tgcctggcgt cggcggtggc
     6361 attattcggt gaaccgaaaa gcgtgcaggc aaccgccagt ttgttggcaa gcggcgtaga
     6421 tgcccacggc gtggtggtga tggattacgg tgatttcagc gttacgttgc agcactcaaa
     6481 ggtcagtgat tctgtcctgg aaagcgagat tcagggcgaa gcgggatcgc tggtgattga
     6541 aaaactgtct gaatgccaga aagtgtgctt cgtgccgcgt ggcagccaga tgcaggatct
     6601 cacccagccg cagcatatta ataccatgct ctacgaagca gagctgtttg ctaccctggt
     6661 ggatgagcat ctggtggatc atccagggct ggcggtcagt cgcatcaccg ccaaactgct
     6721 gactgagatc cgccgccaga ctggggtgat ttttccggca gatagcgtaa aactataatt
     6781 gccaaagtaa aacagtgtaa aaggtatgta acagaccatt gactggctga atggtctgtc
     6841 atactttgtt acctgcaaag gggagtaact tcattgccgg tcgatcgtca ttacgatgtg
     6901 tgaaaaacca catccggtca ccgggcaacc cgaaaggaat acgcagacgt attccttttt
     6961 tgttgtaagt gagaccttgc cggaaggcga ggtctatgca taaaaagcag cggctgacgt
     7021 cttccgacgt tggccgtttt tttatgtgta aggaacttct atgaatactg tcggcacgcc
     7081 gttgctatgg ggcggattcg ctgttgttgt cgccattatg ctggctatcg acctgttgtt
     7141 gcaggggcgt cgtggggcac atgccatgac catgaaacag gctgcggcct ggtcgctggt
     7201 ctgggtgacg ctgtcgttac tgtttaacgc cgctttctgg tggtatctgg tgcaaactga
     7261 aggtcgcgcc gttgccgatc cacaggcact ggcctttctc acgggttatc tgattgagaa
     7321 atcgctggcg gtcgataacg tctttgtctg gctgatgttg ttcagctatt tctctgttcc
     7381 ggcggcatta caacgccgcg tgctggtgta tggcgtgctc ggggcgattg ttctgcgtac
     7441 catcatgatc ttcactggca gctggctaat ttcgcagttc gactggatcc tgtatatctt
     7501 cggtgccttc ctgctgttta ccggcgtgaa gatggcgctc gcccatgaag atgaatcagg
     7561 cattggcgac aagccgctgg tgcgctggct acgcggtcat ttgcgcatga ccgacaccat
     7621 cgacaacgag catttctttg tgcgtaagaa tggattgctg tacgccacac cgctgatgct
     7681 ggtactgatt ctggtggaat tgagcgacgt gattttcgcc gtggatagca ttccggctat
     7741 cttcgccgtg accactgacc cgttcattgt gctgacctca aacctgtttg cgatcctcgg
     7801 cctgcgtgcg atgtatttcc tgctggcggg cgtagcagag cgtttctcga tgctcaaata
     7861 tggcctggcg gtgattctgg tgtttatcgg tatcaagatg ctgattgtcg acttctacca
     7921 tattccaatc gccgtctcgc tgggcgtggt gtttggcatt ctggtgatga cgtttattat
     7981 caacgcctgg gtgaattatc ggcatgataa gcagcgggga gaatgattcg ctgtatatta
     8041 agccattcct aga
//
LOCUS       DAJVYM010000158         7967 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVYM010000158 DAJVYM010000000
VERSION     DAJVYM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.160"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..631
                     /locus_tag="OQS60_003387"
     CDS             <1..631
                     /locus_tag="OQS60_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS2389063.1"
                     /translation="PAAQQGLPAHGFARNLPWTLKSHNEDADGVALTFELTQSEETKK
                     FCPHDFTLLAHFRVGKTCEIDLESHGEFETTSALHTYFNVGDIAKVSVSGLGDRFIDK
                     VNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVVGWNPG
                     PALSISMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(682..1536)
                     /gene="yeaE"
                     /locus_tag="OQS60_003388"
     CDS             complement(682..1536)
                     /gene="yeaE"
                     /locus_tag="OQS60_003388"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS2389064.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1626..2372)
                     /gene="mipA"
                     /locus_tag="OQS60_003389"
     CDS             complement(1626..2372)
                     /gene="mipA"
                     /locus_tag="OQS60_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS2389065.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2808..4742
                     /gene="yeaG"
                     /locus_tag="OQS60_003390"
     CDS             2808..4742
                     /gene="yeaG"
                     /locus_tag="OQS60_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS2389066.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4855..6138
                     /locus_tag="OQS60_003391"
     CDS             4855..6138
                     /locus_tag="OQS60_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCS2389067.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6285..7760
                     /locus_tag="OQS60_003392"
     CDS             6285..7760
                     /locus_tag="OQS60_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS2389068.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2122 a         1904 c         1842 g         2099 t
ORIGIN      
        1 tccggcggca caacaaggtc tgcctgcgca cggttttgcc cgcaacctgc cgtggacgct
       61 gaaatcacat aatgaagatg ctgatggcgt agcgctgact tttgaattga cgcaaagcga
      121 agagacgaaa aaattctgtc cgcacgactt tacgctgtta gcgcatttcc gcgtgggtaa
      181 aacctgtgaa atcgatcttg aatcacatgg cgaatttgaa accacctctg ccctgcatac
      241 ctactttaac gtgggtgata tcgctaaggt aagcgtcagt gggctgggcg atcgcttcat
      301 tgataaagtg aatgacgcga aagaagatgt actgaccgac ggtattcaga ccttccctga
      361 ccgtaccgat cgcgtgtatc tgaatccgca agattgcagc gtgattaatg atgaagcgct
      421 gaatcgtatt atcgccgtag gccaccagca tcatctgaac gttgtaggct ggaacccggg
      481 accggcgctt tcaattagca tgggcgatat gccggatgat ggctacaaaa catttgtttg
      541 tgtagaaacg gcttacgctt cagaaacgca aaaagtcacc aaagagaaac ctgcacatct
      601 ggcgcaatcc attcgcgttg cgaaacgtta atttgcgtta atgttgtgtg ccgggtgcga
      661 tgcatccggc acgtatcaac atcacaccat atccagcgca gtttttcctt ttggtgccgg
      721 atacgcttta tccagcatgg ttaattccgc tgacgaaagt tcgacctcaa gcacagccgc
      781 attctgttga acatgggcaa tcgtggccgc ttttggaatc gccatcacac cctgatggct
      841 gattacccac gccaacaata cttgtgccga gctgatattg tgagcatgtg caatttcgtt
      901 gactaccgcg ttttttaaca gtccattgcg caaccgcccg gcctgggcta atggactgta
      961 agccatcacc ggcatctgct gttgctggca ccaggggagt agatcgtact caatccctcg
     1021 tgaaccgaga tggtaaagca cctgattagt ggcacactga tttccccccg gcagctgcca
     1081 gagttcctgc atatcagcat aatcaaggtt agaaacgccc cagcggcgga tttttccctg
     1141 ggcgatcaat ttttccatcg ctgcaacagt ctcttcaaaa gcgaaactgc cagacctgtg
     1201 taataagtag agatcgagat aatcagtatt gagacggcgt aaactggctt cgcatgcatt
     1261 tatcgctttt tgcccgccag cattccacgg atagacttta gagacgagaa agacgttatc
     1321 acgcagaccg gttaatgctt ccccaaccac cttttcggca ccgccatcgg catacatttc
     1381 ggcggtatca atgagggtta aaccgagctc aatgcccgcg cgtagtgcag caacttctgt
     1441 tttgcgctga ctggcatctt cgcccatata ccatgttccc tgccctatgg ctggcagtga
     1501 gacatcgcca ctaaattgaa tcattttttg ttgcattgtt tcctcccagg tattgcacca
     1561 ccgtaatgca aaacagggcg tgacgccctg tttttatgca caaaatgccc tggaaagatg
     1621 cattatcaga atttgtaggt gatcccggta gaaatcaggc cagtccagga tttatccacc
     1681 atcgggctgt cagtaacttc atcagacaga cgggtatagc gcgcggtacc gtaaacactc
     1741 cagtcgccga ggaagttgta gctggcgctc agctccaggt aagggctcca gctgtcgttc
     1801 gggttatagc cacgcagacc gctgcgagcg gactctttgc gcgatacgcc ataatagtat
     1861 tcgttctggt tttcgctgtt ccactgcaca ccgatacccg gggtcactgt caggccgccg
     1921 ttggtgtaac gatacaacca ggccatatcc cagacaatgc cgttgctgtt atccagggta
     1981 tcgccagtca gggtggtacg caggtaaccg tactgggtaa agtgagcata agacagacca
     2041 gccatcatgg tgctcttacg gtcatccagg tgacgcattt ggtgatcgcc actgtctttc
     2101 gctttgaagt aaagcggcga ccaataagcg gtaattgaaa gtttgtccgt tgcgtcattc
     2161 cacaggtagt aaccaccacc taagccacgg aaccagaagt tatcgccttc atagttgatt
     2221 accggtactg ggtaaacatc ggtatcgtaa tctttatatg ggtgttcaac gacaccgacg
     2281 cctgcgccca gggaaaattt accttcagcg tgcgctacgc ctgcagacgt tgcgataagc
     2341 actccaagtg ccagaagttt gagtttggtc acaattaatc attccttaaa caaatgttta
     2401 gcgggcgaca aagtttaccc gtcaatacat cgaaacccaa cctttttacg ttttcatttt
     2461 ttaaagtaac tgtttaattt tcctgacgcg gatgacaccg cgcttacagc caaatgaatt
     2521 tagcgttact ggcgagcctt gtctttacat taattatgca aaatttatgg atgagttgtt
     2581 gatatgccat tgaaattaag aaagccgtgc aggcaagttt tccatttgcc atctacgctt
     2641 aattttgaag gtgtatcacc gggcacgttg ttctcatcgt cgataaaatg gcatgagagt
     2701 tgctgtgttt tagcaagaga cgtcgttcag tttacctctt ccgggagcct ctactattca
     2761 tatgaacggc tcttaacctg tgctaaaaaa cgaaaggacg gcataccatg aatatattcg
     2821 atcactatcg ccagcgatat gaagctgcca aggacgaaga gttcacactg caggagtttc
     2881 ttaccacttg tcggcaagat cgcagtgctt atgccaacgc ggctgagcgg ctattgatgg
     2941 ctatcggtga gcctgtcatg gtcgatacag cccaggaacc cagactttct cgactctttt
     3001 ctaaccgggt cattgcacgt tatccggcat ttgaagagtt ttacggcatg gaagacgcga
     3061 ttgaacagat tgtctcttat ctgaaacacg cggctcaggg gctggaagag aagaaacaaa
     3121 tcctgtatct gctggggcct gtgggtgggg gtaaatcatc gcttgctgag cgactgaaat
     3181 cattaatgca gctcgtaccg atttatgtat tgagcgccaa cggtgagcgt agcccggtca
     3241 acgatcatcc gttctgtctt ttcaatccgc aggaagatgc gcagattctg gaaaaagagt
     3301 atggcattcc tcgccgctat ctcggcacca tcatgtcgcc gtgggcggca aaacgcctgc
     3361 atgaatttgg tggcgatatc actaagttcc gggtagtgaa ggtctggccg tcaattctgc
     3421 aacaaattgc tatcgccaaa acagaacccg gtgatgagaa caaccaggac atctccgcgc
     3481 tggtagggaa agtcgatatt cgtaaactcg aacactacgc gcagaatgac ccggacgcct
     3541 acggctattc cggtgcgctg tgccgcgcca atcaggggat catggaattt gtcgagatgt
     3601 ttaaagcacc gattaaagtg ctgcatccgc tgttaaccgc tactcaggaa ggtaactaca
     3661 acgggacgga aggtatctcc gccctgccgt tcaacgggat tattctcgcc cactcgaacg
     3721 aatccgaatg ggtcactttc cgtaataaca aaaacaacga agccttcctc gaccgtgttt
     3781 acatcgtgaa ggtgccgtat tgcttacgca tttccgaaga gatcaaaatc tacgagaaat
     3841 tgcttaatca cagtgaattg actcacgccc catgcgcccc tggcacgctg gaaacactgt
     3901 cacgtttttc cattctttcg cgcctgaaag agccagaaaa ctccagcatt tattcaaaga
     3961 tgcgggttta tgatggcgaa agtctgaaag acaccgatcc caaagccaag tcgtatcagg
     4021 aatatcgtga ctacgccggt gtcgatgaag ggatgaaagg tctgtcgacg cgttttgcgt
     4081 ttaagatcct ctcccgcgtg ttcaacttcg atcatgtaga agtggcggca aacccggtcc
     4141 atctgttcta cgtcctagaa cagcagatcg agcgcgagca gttcccgcaa gagcaggcag
     4201 aacgctatct ggagttcctg aaaggttatc tgatcccgaa atatgccgag tttatcggca
     4261 aagagatcca gacggcctac cttgaatcct attccgaata tgggcaaaac attttcgacc
     4321 gttatgttac ctacgcggat ttctggattc aggatcagga gtatcgcgat ccggataccg
     4381 ggcagctgtt tgaccgcgag tctcttaacg ccgagctgga gaaaatcgag aaaccggcgg
     4441 ggatcagtaa tccaaaagat ttccgcaacg agattgttaa cttcgtactg cgcgccagag
     4501 cgaataacag cggacgcaat ccgaactgga ccagctatga aaaactgcgc acggttatcg
     4561 agaagaaaat gttctccaat accgaggagc tgttgccggt tatctcgttt aacgccaaaa
     4621 cgtcaaccga cgagcagaag aaacacgacg actttgtcga ccgtatgatg gaaaaaggct
     4681 acacccgtaa acaggtgcgt ttactgtgcg aatggtattt gcgcgtacgt aaatcgtctt
     4741 aacaaccctg gcccggtaca catgctaccg ggcctataac gacagcgaac cgtgggccag
     4801 agaaatggca acacaggcgt agcatacagt tggcaaatgt agtacggggg gcatatgacc
     4861 gggtttattg accggcgtct gaacggcaaa aacaaaagca tggtgaatcg ccagcgtttt
     4921 ttacgccgtt ataaagcgca aattaaacag tcgatctccg aggccattaa taagcgttcg
     4981 gtgactgacg tcgacagcgg cgaatccgta tccattccca cggaagatat tagcgaaccg
     5041 atgtttcatc aggggcgtgg cggcctgcgc caccgcgtac atccgggcaa tgaccatttc
     5101 gtccagaacg accgaattga acgtccccag ggtggcggcg gaggttccgg cagtggtcag
     5161 ggccaggcca gccaggatgg tgaaggtcag gatgaatttg tctttcagat ttcgaaagat
     5221 gagtatcttg atctgctctt tgaagatttg gccttaccga atctgaaaca aaaccaacaa
     5281 cgccagctga ccgaatataa aacgcatcgg gcgggttata ccgctaacgg cgttccagcc
     5341 aatatcagcg ttgtgcgttc cttgcagaac tcactggcgc gacgcacagc catgacggcg
     5401 ggcaagcgac gggaacttca tgcgctggaa gagaatttgg ccatcatcag caacagtgaa
     5461 cctgcgcaac tgctggaaga ggaacgtctg cgcaaagaaa ttgcagaatt acgtgccaaa
     5521 attgaacgcg tcccttttat tgacaccttc gatttacgtt acaagaacta cgagaagcgg
     5581 cccgatccct ccagccaggc agtgatgttt tgcctgatgg acgtttccgg ttcaatggat
     5641 caatccacta aagatatggc taagcgtttt tatattctgc tgtatctgtt cctcagcaga
     5701 acgtataaga acgtggaagt cgtatacatc cgccatcata cccaggcgaa agaagtcgat
     5761 gaacatgagt ttttctactc gcaggaaaca ggcggcacca ttgtttccag cgccctgaaa
     5821 ctgatggatg aggtagtgaa agagcgttat aacccggcac agtggaatat ttacgctgca
     5881 caagcatcgg acggcgataa ctgggccgat gactctccgc tttgccatga aatcctggcg
     5941 aaaaaattat tacctgttgt tcgttattac agctatatcg aaattacccg tcgtgcacat
     6001 cagacattgt ggcgagaata tgagcatctg caatctactt tcgacaactt tgcgatgcag
     6061 cacatccgcg accaggatga tatttatccg gtgttccgtg aactgtttca taaacaaaat
     6121 gcaacagcta aagactaaaa ctatcagcca ggtcattatc gcctggctga tttttagctt
     6181 actgtaaatt atctcattta ttacatacat tagcttacaa tcgctttaaa tatgacagca
     6241 taacctttac ataatttagt tccagaaaac aatcattcgg aaaaatgatt cagtcaacac
     6301 gtatttccat ggggttattc tttaaatatt ttttatcgtt aacgaaaatt gatcctggtc
     6361 aaaactatat atctctgcca tcaataaaat ccagcactca cattgctctc ctttttatgg
     6421 tttctatggg tacacaaaaa ttaaaagctc aaagcttttt tattttcagt ttattgctga
     6481 cgttaatttt attttgcatt actaccttat ataacgaaaa cacaaatgta aaactcatcc
     6541 cacagatgaa ttacctgatg gttgttgtgg ctttgttttt ccttaacgcc gtcatttttc
     6601 ttttcatgtt aatgaaatat ttcactaaca aacaaatttt accaacactc attttaagcc
     6661 ttgcattttt aagtggcctt atctatttag ttgaaaccat tgtaattatc cataaaccaa
     6721 ttaacggcag tacactgatc cagacaaagt cgaatgatgt ttctattttc tatattttcc
     6781 gccaactcag ttttatttgt ttaacctcgc tggcactctt ttgttatgga aaagacaaca
     6841 tccttgacaa caataagaaa aaaacgggaa tcctgttgct ggcgctgatt ccttttttag
     6901 tttttcccct tctggcacac aatctgagca gttataacgc tgactattct ttgtatgtcg
     6961 tcgactactg tccggacaac catactgcga cctggggaat caactataca aaaatattgg
     7021 tttgtctgtg ggcattttta ctgttcttta ttatcatgcg tacacgatta gccagcgaac
     7081 tatggccgtt aatagcatta ttatgtctgg catcgctatg ctgcaactta cttctactga
     7141 ctctggatga gtataattac accatctggt atatcagtcg cgggattgaa gtttccagta
     7201 aactgtttgt tgtgtctttt ctgatttata acatttttca gaaactgcaa ctctccagca
     7261 aactggcagt tcatgatgtg ctgaccaata tttataatcg gcgctacttt ttcaacagcg
     7321 tagagtcatt attgtcgaga cctgttgtta aggacttctg tgtcatgctg gttgatatta
     7381 atcagttcaa acgcatcaat gcccaatggg gacatcgtgt gggtgataaa gtgctggttt
     7441 caattgtcga tattatccag caaagcatcc gccccgatga tattttagcg cgactggagg
     7501 gtgaggtgtt tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg
     7561 aacgtatgcg taaaaatgtc gaactcctga ccggctttag taacagatat gatgttcctg
     7621 aacaaatgac catcagtatt ggcacggttt tttcaacggg tgacacgcgt aatatctcgc
     7681 ttgtcatgac ggaagcagat aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag
     7741 tgattattca tcatatttaa gtgcaaaaat attcagagcc atgctttttg cgtggctttt
     7801 gcatacaatt tattactatt acccctaaat ttcccctccc cagcggcgca gagatgagta
     7861 taattagcgc ccctgtgcca ggccgcaatc gaactttatc tggttttctc gtttcactaa
     7921 ccgaaggagt gccatttatc atgaaattgc accatagaat gctccgg
//
LOCUS       DAJVYM010000159         7957 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVYM010000159 DAJVYM010000000
VERSION     DAJVYM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.161"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1556
                     /locus_tag="OQS60_003393"
     CDS             <1..1556
                     /locus_tag="OQS60_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS2389069.1"
                     /translation="SSLLDALTLLLSPESDLYHFERDDFWFPPGDINGREHHLHIILT
                     FRESLPGRHRVRRYRPLEACWTPCTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHP
                     IDVEDINDQARHLVRLMPVLRLRDARFMRRIRNGTVPNVPNMEVTARQLDFLARELSS
                     HPQNLSDGQIRQGLSAMVQLLEHYFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRM
                     IDRPGGRSYRVILLGLFATLLQAKGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLL
                     NLLPLQRIVTTNSGELLSLTPVEHVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIR
                     FNRPSSLFARCWLLVEGETETWVINELARQCGHHFDAEGIKVIEFAQSGLKPLVKFAR
                     RMGIEWHVLVDGDEAGKKYAATVRSLLNNDREAEREHLTALPALDMEHFMYRQGFSDV
                     FHRVAQIPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRV
                     LWLARGRAD"
     gene            complement(1553..2509)
                     /locus_tag="OQS60_003394"
     CDS             complement(1553..2509)
                     /locus_tag="OQS60_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS2389070.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2660..3775
                     /gene="macA"
                     /locus_tag="OQS60_003395"
     CDS             2660..3775
                     /gene="macA"
                     /locus_tag="OQS60_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS2389071.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3772..5718
                     /gene="macB"
                     /locus_tag="OQS60_003396"
     CDS             3772..5718
                     /gene="macB"
                     /locus_tag="OQS60_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS2389072.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5791..6015)
                     /gene="cspD"
                     /locus_tag="OQS60_003397"
     CDS             complement(5791..6015)
                     /gene="cspD"
                     /locus_tag="OQS60_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS2389073.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6420..7658
                     /locus_tag="OQS60_003398"
     CDS             6420..7658
                     /locus_tag="OQS60_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS2389074.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         1958 a         1898 c         2146 g         1955 t
ORIGIN      
        1 aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat ctctaccatt
       61 ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa catcatctgc
      121 atattatttt gaccttccgc gaatcgctgc caggccgaca tcgggttcgc cgttatcggc
      181 cgctggaagc gtgctggacg ccatgcaccg atggctatca ccgtattttt tatcgtctgg
      241 aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc gataaagacg
      301 gacatccgat tgatgtcgag gatattaacg atcaggcacg ccatctggtg cgtttaatgc
      361 cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa
      421 atgtccctaa tatggaagtc accgcgcgcc agctcgattt cctcgcccgt gagttatcct
      481 cacatccgca aaatctctct gatgggcaga ttcgtcaggg actttccgca atggtacagc
      541 tgcttgagca ttatttctct gagcaggggg ccggacaggc gcgatatcgt ttaatgcggc
      601 ggcgagccag caatgagcaa cgaagctggc gctatctgga tatcatcaac cggatgattg
      661 accgacctgg tgggcgctcg tatcgggtta ttttgctcgg cctgtttgct actttgttgc
      721 aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg atcgaagatc
      781 cagaaacccg tttacacccc attatgcttt cagttgcctg gcatctgttg aatcttctgc
      841 cattgcagcg cattgtcacc accaactcgg gtgagttgct ttcgttaacg ccggtagagc
      901 atgtttgccg actggtacgt gagtcctcgc gcgttgccgc ctggcgtctg gggccgagtg
      961 gcttgagtac cgaagatagc cgacgcatat cctttcacat tcgttttaac cgtccgtcat
     1021 cgctgtttgc acgctgctgg ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg
     1081 aactggcgcg tcagtgcgga catcattttg atgccgaagg gatcaaggtc attgagtttg
     1141 cccagtccgg gctaaagcca ctggttaaat ttgcccgccg aatggggatt gaatggcatg
     1201 tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc agcctgttga
     1261 ataacgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg gatatggaac
     1321 attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa
     1381 atgtaccgat gaatctacgc aaaattatct cgaaagcgat ccatcgctct tccaaacccg
     1441 atcttgccat tgaagtggca atggaggcag gacgtcgtgg tgtggactcc gtaccgacgc
     1501 tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc
     1561 gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt
     1621 cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt gctggaaggc
     1681 ggtaatggcg ttcagcatca catacgccgc caaccgattc ccagaaagcg ttgtaatcag
     1741 cgtggatctt gccttcttta tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc
     1801 tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggcc gcttccatca
     1861 cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg
     1921 gaatttccca ttttgcgcct tgcagtccgc caataaacat cgttcttttc ccctgatatt
     1981 cacacagggt aaacgtgatc tctgccagag gaataccttc gctgttgcga aacaggattg
     2041 tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagcgtg aactgctcgc
     2101 cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat aagctgaatt
     2161 cttccgccga catacatccg cggagtaacg cataatggta acgtaacgcc tccagcaatt
     2221 gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc agtcgcggtt
     2281 ggcgcgttaa caacacattg agattaggcc agtgggaaag ttcgttcatc cactcaacgc
     2341 ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc
     2401 agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac aggctgctga
     2461 gagattcaga tggcgtaaag gtccgttcag ttagctgcga catattcatg aaatcaatgg
     2521 ttatacatga cgtcgatttc accattgcgt atcttaacca aacatcaata gtgtgattac
     2581 taacgtaaat tttatggttt tgttgatatt tcgttgaagt taatgacccg gattggcata
     2641 tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc
     2701 gctggtgata gtcatcgccg gactgattac gttatggaga attcttaacg cacccgtgcc
     2761 gacttatcag acactgattg tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg
     2821 aaagctggac gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac
     2881 gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg tcattgatcc
     2941 tgaacaggct gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca
     3001 gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc gtcagcaacg
     3061 tctggcacaa acgcaggcgg tttcacagca ggatctcgac accgccgcga cggagatggc
     3121 tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc aggcttctct
     3181 cgatacggct aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg ccggggaagt
     3241 cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag caccgaacat
     3301 tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg aagcggatgt
     3361 aatccacctg aagccggggc aaaaagcctg gtttacggta cttggcgatc cactgacgcg
     3421 ctacgagggg caaatcaagg atgtactacc gacgccggaa aaggttaacg acgctatttt
     3481 ctattacgcc cgttttgaag tccccaaccc caatggtttg ctgcggctgg atatgactgc
     3541 gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt
     3601 aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg gcgaaacacg
     3661 cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg tcaaagggct
     3721 ggaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac aatgacgcct
     3781 ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtaatgagca ggttgaggtg
     3841 ctgaagggca tcagcctcga tatttatgcg ggtgagatgg tcgcgattgt tggcgcttcg
     3901 ggttccggta aatcgaccct gatgaatatt ctcggctgcc tggataaggc caccagcggc
     3961 acctatcgcg tagccggtca ggatgttgcc acgctggacg ccgatgcgct ggcgcaactg
     4021 cgccgcgagc atttcggctt tattttccag cgttaccatt tgctttcgca tttaaccgcc
     4081 gagcagaacg ttgaagtacc cgccgtctat gctggtcttg agcggaaaca gcgactgctt
     4141 cgtgcccagg agttgctgca acggctgggg ctggaagacc gtacagagta ttatccggca
     4201 cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc gggcattgat gaacggcggt
     4261 caggtaattc ttgccgatga accaaccggc gcactggaca gccattctgg cgaagaggtg
     4321 atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt cacccacgat
     4381 ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc
     4441 aatcctcccg ccattgaaaa agtgaatgtt gctggcggga cggagcctgt tgtcaataca
     4501 gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg
     4561 gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat tatcggtatt
     4621 gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca aacaaatggt gctggcggat
     4681 attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt tggcgatgac
     4741 gatccgcaat atcagcaggc gctgaagtac gacgacttaa tcgccatcca aaaacaaccg
     4801 tgggtcgcct cagccacacc tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt
     4861 gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc
     4921 ttcagtgaag gaaacacctt taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt
     4981 ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt tggcgaggtg
     5041 attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa acagtcgatg
     5101 tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt
     5161 atgggccagt cgtggcttaa ctccattact gtcagggtga aagaaggatt tgacagcgcc
     5221 gaggcggaac agcaactcac gcgtttactt tcactgcgcc acggtaagaa ggatttcttt
     5281 acctggaaca tggacggcgt cttgaaaact gttgaaaaga ccacacgtac tttacaactg
     5341 tttctgacgc tggtggcggt gatttcgctg gtggtgggtg gtattggtgt aatgaatatt
     5401 atgctggtgt cagtgaccga gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg
     5461 cgagcaagcg atgttttgca acagttcctg atcgaagccg tactggtttg cctggtcggt
     5521 ggcgcgttgg gaataactct gtcactgtta attgctttca ccttgcagct tttcttaccc
     5581 ggctgggaga ttggtttttc accgttggca ctgctgctgg cgtttctctg ctcgacggcc
     5641 accgggattt tatttggatg gttacccgca cgaaatgcgg cacgactgga tccagtagat
     5701 gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcgggct ggcattttgc
     5761 ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg
     5821 cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg
     5881 tccagctttt agcgttctat aaccatccat ctgaatggtg gaataatgag cgaaaatatc
     5941 ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac
     6001 agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa
     6061 ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa
     6121 ttagggcgag ccgtcaagca tttgacaggg gccagggggc aggtatgaat caaaaatttg
     6181 aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat
     6241 agttatttat catctagtgt tcgcgggtgt tcgtggtagt gttttgtgtg cattcatcgc
     6301 tatgattata aaggaaaatt ttgttttcct tgttcgctct tgttcgtcac ggtttgccct
     6361 tgtacgatgc acggtgtagt caatggtgta gttaattttg atgaccggaa acgcgaaaca
     6421 tgagcagcgc aattcataga cttagcgaca ctcttttacg aaaattaagc ggatcaccaa
     6481 ccacaaaaaa cgcctttttt aatgacggtg gcaatcttag cgtaagacat tccaccagcg
     6541 gcctgttaac ctggtatttc acttacaggg ccggaactgg caggcaggta tcaccggaac
     6601 gcctgagact gggcaactat cccgatctga gcctgaaagc agccagggaa aaagcggcac
     6661 agtgtcgcgc ctggctggct gaggggaaaa atccgcgcta tgagcttaat cgtgctgtac
     6721 aggatgcgtt agcccccgtg acagttaagg aagcgctcac ttactggctt gaatcgtatg
     6781 caaaggaaaa gcgcactgac tacgaatcac tgaaaagccg gatcaataaa cacataatca
     6841 gccagattgg cgctttgccg ttggaaaaat gcgagctacg gcactggttg gcatgttttg
     6901 accagatggc aaagcgaagc ccagtatctg ccggattttt gctacaggta tgcaaacagg
     6961 cgcttaagta ctgccgaaaa cgacgatacg caatcagcaa cgttcttgat gatatggttg
     7021 tcggtgatgt gggaaaaaaa gcagaaataa gcgagcgcgt actaacaaac aaggagcttg
     7081 gggaattact ccgcgccctg gatgaaaaaa tttacccacc gtactacagc gccctgattc
     7141 gcctcctgat tgtcttcgga tgccgtgcca ctgagctaag acgctctgag gtccaggaat
     7201 gggattttaa agaaatgctc tggaccgtgc cgaaagaaca cagcaaaacg aaggtagcaa
     7261 tattcaggcc aataccggaa gcgatcttgc cgttcgtaac gaagctggtg gagcagaaca
     7321 ggcacacggg attattgctc ggagaactga aagggcaatc atccgtatca gagtacggaa
     7381 gaacggcaca cagacgcatt aatcaagccc cctggacgtt gcacgacatc cggcacacgt
     7441 ttacaacgat gctgaacgat ttaggcgtgg atcctcatgt agtggagcag ctaaccgcgc
     7501 accagttgcc aggagtgcaa cgagtctata atcactcccg ttatctcgat gcaaaacgtg
     7561 acgctcttaa tctatgggtt gagcgtctcg agcttctcca gaacaatgat gaaaaaatcg
     7621 ttgttatgac cccgcgaatt tactcccaaa attcttgaca aattacggct gtttttcttg
     7681 cgaaaaatgg ccctctaaaa taaatcgtcg aatatcggca aatatcgacg atttatatgt
     7741 gtcaactatc gaaaaaaatt gaaatgtctt cttctgagtt cttattctct gtttcttgat
     7801 tattccaata cctgatggtt tatctatttg tttttaatga ggtaattcag gtgtcatgcc
     7861 aaatggcgac attttttgtt gcgtcgctta aaaactcgtg acacgatttt aaacactaac
     7921 gagcttctac gaaccttagc gaacaaagcc tttagca
//
LOCUS       DAJVYM010000160         7944 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVYM010000160 DAJVYM010000000
VERSION     DAJVYM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.162"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            111..443
                     /locus_tag="OQS60_003399"
     CDS             111..443
                     /locus_tag="OQS60_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS2389075.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="OQS60_003400"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="OQS60_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS2389076.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="OQS60_003401"
     CDS             complement(2281..2598)
                     /locus_tag="OQS60_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389077.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="OQS60_003402"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="OQS60_003402"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS2389078.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="OQS60_003403"
     CDS             complement(3985..4758)
                     /locus_tag="OQS60_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2389079.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OQS60_003404"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OQS60_003404"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS2389080.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="OQS60_003405"
     CDS             complement(5952..7307)
                     /locus_tag="OQS60_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS2389081.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OQS60_003406"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OQS60_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS2389082.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OQS60_003407"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OQS60_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS2389083.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAJVYM010000161         7936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVYM010000161 DAJVYM010000000
VERSION     DAJVYM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.163"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQS60_003408"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQS60_003408"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS2389084.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="OQS60_003409"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="OQS60_003409"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS2389085.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="OQS60_003410"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="OQS60_003410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS2389086.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="OQS60_003411"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="OQS60_003411"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS2389087.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="OQS60_003412"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="OQS60_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS2389088.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="OQS60_003413"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="OQS60_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS2389089.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="OQS60_003414"
     CDS             5724..5885
                     /locus_tag="OQS60_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS2389090.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="OQS60_003415"
     CDS             6007..7080
                     /locus_tag="OQS60_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS2389091.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="OQS60_003416"
     CDS             7077..7859
                     /locus_tag="OQS60_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS2389092.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1895 a         2047 c         2152 g         1842 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc atatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gttttgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc
     2581 ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggatgc cggtaagact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggctcct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 agcgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt gatcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc ggtctggatc
     7381 tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtatacac gacaaactgg
     7501 cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagc
     7861 ctacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctac
//
LOCUS       DAJVYM010000162         7910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVYM010000162 DAJVYM010000000
VERSION     DAJVYM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.164"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OQS60_003417"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OQS60_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS2389093.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OQS60_003418"
     CDS             complement(2362..2802)
                     /locus_tag="OQS60_003418"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2389094.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQS60_003419"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQS60_003419"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS2389095.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OQS60_003420"
     CDS             3520..4218
                     /locus_tag="OQS60_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2389096.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OQS60_003421"
     CDS             4447..5655
                     /locus_tag="OQS60_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389097.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OQS60_003422"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OQS60_003422"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS2389098.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OQS60_003423"
     tRNA            7762..7838
                     /locus_tag="OQS60_003423"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
BASE COUNT         1814 a         1943 c         2099 g         2054 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc
//
LOCUS       DAJVYM010000163         7873 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVYM010000163 DAJVYM010000000
VERSION     DAJVYM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.165"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..564)
                     /locus_tag="OQS60_003424"
     CDS             complement(<1..564)
                     /locus_tag="OQS60_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS2389099.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQ"
     gene            complement(596..1984)
                     /locus_tag="OQS60_003425"
     CDS             complement(596..1984)
                     /locus_tag="OQS60_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS2389100.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRS"
     gene            complement(1995..2714)
                     /locus_tag="OQS60_003426"
     CDS             complement(1995..2714)
                     /locus_tag="OQS60_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS2389101.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3242..4096)
                     /gene="rclR"
                     /locus_tag="OQS60_003427"
     CDS             complement(3242..4096)
                     /gene="rclR"
                     /locus_tag="OQS60_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCS2389102.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4322..5647
                     /locus_tag="OQS60_003428"
     CDS             4322..5647
                     /locus_tag="OQS60_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCS2389103.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5756..5992
                     /locus_tag="OQS60_003429"
     CDS             5756..5992
                     /locus_tag="OQS60_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2389104.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6004..6597
                     /gene="rclC"
                     /locus_tag="OQS60_003430"
     CDS             6004..6597
                     /gene="rclC"
                     /locus_tag="OQS60_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCS2389105.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6757..7626)
                     /locus_tag="OQS60_003431"
     CDS             complement(6757..7626)
                     /locus_tag="OQS60_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS2389106.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2134 a         1918 c         1687 g         2134 t
ORIGIN      
        1 ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca cgcggcagga ttgtgctttt
       61 acgcaggata aaaagagaag attccgggag caggctcaat gaacgcccgc gctcgacggc
      121 ggaaaaaaga accacgcctc ccgattcggt taaaccatat tcagcataca cgacacccac
      181 tttagcctgc tctgcctgcg agatattctc ggcacctttc gccggatccc aaacaacggc
      241 attgcattcc tgctgcaaac gttcactaat ccccaattcc tccagcctcg tgtcaccgct
      301 aatcacgacc gactgatctc ccagctcttt acacagacgt attgcagctt ctgccgcctt
      361 cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg ctggcaaact gaataaacgc
      421 gtcacagcgc tgctgttggt taagttgggt aagccgctcg ttagcatagt tgttaagcgg
      481 cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc agtgcctgag caacgttatt
      541 caaaaattcg ctccgattat ccattctttt tctcctgcgc ctgatgtttc ttaaactaac
      601 tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc ttccatccag tcgctaatcg
      661 cgccaaattt gagtggtgtt ttgccgccat tgataaacca gcttgccgca tgagcgccgg
      721 ccatcatccc gactttccac aatcctggat gactattggc ataagcgaac atttttatcg
      781 cccgttgctc tgcttttgcg gtgatccctt tttcagccat cacccgacga tgacgcaaaa
      841 tcagttttga cagcggaata cgcaccggac acacgctgtc acaagcagtg cataaagagc
      901 aggcgtaggg taaatcttta aaatctttat agccgccaag tagcggagaa atcaccgcac
      961 caattggccc tggataaata gagccatatc catgaccgcc aatatggcga tatgccggac
     1021 aagtattcat acaagccccg cagcgaatac agcgcagcac atcccgaaat tcagaggcca
     1081 gcacctcaga acgcccgtta tcgacaataa ccagatgaaa ctcttcagga ccatcaacgt
     1141 gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc cgtcaaacgt gcaccaacgg
     1201 cactgcgcgc cagcatggtg atcaatacat ctacctcggc aaacgtgggg gcaatacgct
     1261 ccattcccat cacagcaata tgcgttttag gcagcgtggt acacattcgc gcattacctt
     1321 cattggtcac caggcatacc gaacctgtct ctgccaccgc gaaattacag ccggtaatac
     1381 ctatttcagc actgaggaaa tcttcgcgga ttttttgccg gatgaataag gtcatcgctt
     1441 caggcgtttc cggcccctca tagcccagac gttcgtgtag cactcgacgg atctgatggc
     1501 gatctttatg aattgccggg accacaacat gagatggcgg atcttgatcc agctggagaa
     1561 tatattcacc cagatcggtt tcaatcacct gaatgccagc atcctgcaac acatgattga
     1621 caccaatctc ttcggtcacc atcgatttag atttcaccac cttccgggca tttttgcgtt
     1681 gggcaacctg taaaatgtag cgggtagcgt cttctttggt ttttgcaaac cagacgtgac
     1741 cgccgttttg cgtcactttt tctgagagct ggtacagata agcgtcgaga ttactcagaa
     1801 catgatcacg tatctgggcg gcccgatcgc gccactcctc ccagtgcccc aattcatcga
     1861 ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt tgccaccgct ttgcgcatga
     1921 tcggatcttc aatttgctga cggatgcgtg tcttaaaatc tgtattactg gttttgatcg
     1981 acatctttat atcctcagcg gctcatcaac acttcagcaa tatgcatcac tttgactttc
     2041 tgcccttccc gttgtaatcg cccactgatg ttcagcaggc aactcacgtc agcaccaatt
     2101 aaatactcag ggcggacttc catcaggtgc acaacctttt ctttcaccat ctcgccggat
     2161 atttcggcca ttttgaccga gaacgtgccg ccaaatccgc agcaggtatc ctgttcagca
     2221 aaggtcaaca gctccagtcc acgcacattt ttcagcagcg taagtggctc gtccttcact
     2281 cccagcttac gggccaggct acaagatggg tgatacacag ctctcccttg caaactggca
     2341 cctacatcga ctacccctaa tttattaaca ataaaagagg tgagatcctg catacgcgcg
     2401 gcaacctttt cggcacgtga tgcccattca ggttcatccg ccagatacgt cgggtaactt
     2461 tttacggcat aggtgcaaga gccagccggt gaaataatgg gatcgtcgtt atcctccagt
     2521 gcggcgatca gatttttcat ccctggaatc gcttctttga tataaccgct attgatcgca
     2581 ggctgaccgc agcatccctg tttctccggg aaatttacgc gacagccgag tttttccagt
     2641 agcagcacgg agtctcgtgc cattcttgat ttcagggcgt caccaataca ggtgacaaag
     2701 aaattgacat tcacaactaa tactccatta cttcatgccc atttatgccc gatggttccc
     2761 ctccttccgg gggatcaatc atcaataaat atggcgttct tttttaattg gctagcaaca
     2821 taccacccct catttgtgtg acaaatgata atctttcatc caaacttcaa atgcaataac
     2881 taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgtt catttttggc
     2941 ataagaaatc catttaaaaa ggctacatcc cttataccta ttcagagcta cttattcagg
     3001 ggttattgag atatacactg atggtgtaca aaaaaatcac cgacaataat cacgtatcaa
     3061 gtaatagtcg gggttttatt taattgaagc aatttagttt gcatttttat ttaaaaactg
     3121 agctagagtc catcagaaaa aaatatataa agcccaaacc accgttggaa atagaaatag
     3181 ttgaaaggct cacaactccc ggaatgcgga ttatctttgc cctgatttct ggcggtttta
     3241 ttcagggtgc aagctgtctg accctttccc gatattctcc cggagtacaa ccaaactcgc
     3301 ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc tactgactca gcgatcacca
     3361 caacagggag catttcccgg gaaaacatct gggccgctat ttgtagacgc aactttgtta
     3421 atacagccag cggcgtggtt ccggaaacat cacggaaaag ctgggcaaaa cttgcccgag
     3481 acatgtgggc gatgctggcc agcgattcga cggtccaggc gtgtcctggc atttccagca
     3541 tttgctgtat taccgcacca agacgtggat gcagaagcaa actgagaatg tttttctctg
     3601 tattaacctg tgcaatccac tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc
     3661 tacacagtgc atccactccc ggcatcgccg atctggattc ctgttgtaaa aacgggatcg
     3721 cctccctcag ccagttatat tccacgctgt ggttaaccgg tgctaaaaac agcgtttccg
     3781 gcaaagacgt taaaaaataa cgcgccgaat gttgcaaccg aagagtgccg cagacaatac
     3841 aggtcgattc attatcgaca tgactcaggc gatgagctga attttgtggt agcaggacca
     3901 catttcccgg gcgtaatgta aaaatctctc ccgtcggcat ttccagcttc gccgctcctt
     3961 gcgttaacgc atgccaacga ataaccgata attccccggc accatgttga agctgccagt
     4021 cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac
     4081 ggctaagggc atccatatca gactcctgag tggtttttga ttacaaacac caacaatctt
     4141 agtaaataac taatctaata ccatcaaatg gttaatcatg atttttgttt tgtgattctg
     4201 attaagaaaa agaaaatacc agacgataag acaaaatatc aagcgttgtg aagaaatggt
     4261 atttgctctt ttgcgtctat agtcatgatg tcaaatgaac gtgtttcgac aggaaatcat
     4321 catgaataaa tatcaggcag ttattattgg ttttggcaag gctggaaaaa cattagccgt
     4381 cacgctggca aaagcaggtt ggcgagtggc tctcatcgaa caatcaaatg caatgtatgg
     4441 cgggacctgt attaatatcg gctgcatccc aaccaaaaca ttggttcatg acgcacagca
     4501 gcacacagat tttgtccgtg ccatacagcg taaaaatgaa gtggttaatt ttttacgtaa
     4561 taagaatttt cataatcttg cggatatgcc caatatcgac gtgatcgacg gccaggcgga
     4621 gtttatcaat aatcatagcc tgcgtgttca tcggcctgag ggaaatctgg aaattcatgg
     4681 cgagaaaatt tttattaata ccggtgcaca aaccgtggtt ccgccaattc ctggaattac
     4741 caccacgcct ggtgtatatg acagcaccgg attacttaat ctaaaagaat tgcctgggca
     4801 tttaggtatt ttgggcggcg gatatattgg cgttgagttc gcctctatgt tcgctaattt
     4861 tggcagcaag gtaaccattt tagaagcagc ttcgctgttt ttgcctcggg aagatcggga
     4921 tattgctgat aatatcgcga cgattttacg cgatcagggc gtcgatatta tcctcaatgc
     4981 ccatgtggag cgaatcagtc accatgaaaa tcaagtgcaa gtgcatagcg agcacgccca
     5041 actggcggtg gatgcactgt taatagcttc cggtcgtcaa ccggctaccg cttcgttaca
     5101 tccagaaaat gccggtatcg cagtaaacga gcgcggggca attgtcgttg acaagcaatt
     5161 acataccacc gcagacaata tttgggcgat gggagatgtt accggcgggc tgcaatttac
     5221 ttatatttca ctggatgatt accgcattgt gcgtgatgag ttgctgggag aaggcagacg
     5281 tagtactgat gatcggaaaa atgtgcctta ttccgtattc atgacaccgc cactgtccag
     5341 ggttggtatg acagaagaac aggccagaga gagtggtgct gatattcagg tggtgacatt
     5401 gcctgtagct gcaattccgc gtgccagagt gatgaatgat actcgtgggg tattaaaagc
     5461 gattgttgat aataaaaccc aacgtatatt aggggcatca ctgctgtgtg ttgactccca
     5521 cgagatgatc aatatagtga aaatggtgat ggatgccggg ctgccttata gcatattacg
     5581 cgatcagata tttactcatc cgtcgatgag cgaatcactc aatgatctat tttcattagt
     5641 caaataaact caaaatcaga cgccagaaca aatattctgg cgtctcagag aaaagaatct
     5701 tattaattct ctgttactct ataacttctt aatcacttca ttgatggtat tttatatgtt
     5761 taaaaaatct gttttatttg caacactatt atctggcgtt atggaatttt ccaccaatgc
     5821 agatgataaa ataattctga aacatatcag cgtctcgtca gtatcagcat caccgacagt
     5881 tctggaggat accattgctg atatagccag aaaatataat gcttcatcct ggaaagtcac
     5941 atcgatgcga attgataata attcaaccgc aacagcagta ttgtataaat aaggatgttc
     6001 acaatggaaa aatacctgca cctgttaagt cggggagata aaattggcct gtcattgatt
     6061 cgtctgagta ttgcgattgt ttttatgtgg attgggttat taaagtttgt cccttacgag
     6121 gcagacagca ttacaccatt cgtcgcaaac agtccactaa tgtcgttctt ttatgaacac
     6181 ccggaagact ataaacagta tctgactcac gaaggcgaat acaaaccaga agcaagggca
     6241 tggcaatcgg ccaataatac ctatggtttt tccaacggtc ttggcgtcgt ggaggtgatt
     6301 attgctctgc tggttttggc taatcctgtc aatcgctggt taggtttatt gggaggactg
     6361 atggcatata ccacgccgct ggtaacactc tcatttttaa tcaccacccc ggaggcatgg
     6421 gtacccgcat tgggtgacgc tcatcatggt ttcccttatt tatccggtgc tggtcgcttg
     6481 gtattgaaag atactctgat gctggcaggt gctgtcatga taatggcaga ttcggcgcgg
     6541 gaaattctta aacaacgcag taatgaatcc agttcaacgt taaaaactga atattgataa
     6601 tccgaacact gtctgttgtt cactttttca ggcgtcaggc cccctttctg atgatgaagc
     6661 ctgacgccat attaccggag atatgacaga gtgaagatca atatcactcc cctgttaaaa
     6721 gattcttgtc gtctcaggta aaatatatga ttaatatcaa tcatgtattt ttacagcaag
     6781 acaaccacga acaaattcag gattaaaatg ttttaccgac tccccaacat aacccaaatc
     6841 aagcgaacta atttgtgcca tttctttctc tgtcagtgag aaatcccaga tagcaaaatt
     6901 ttcttcgata cgctgttgat gtgtcgattt cggaataacg accactcccc gttgaatatt
     6961 ccaacgcaga atgacttgag aaatcgattt ttgatgggca tctgcaatac tctgaagcag
     7021 attattttca aagggtttat gtcgtccacc acctaacgga gcccatgctt caggctgcac
     7081 gttataatac ttcatggttg ccagcgcctc tggttgggca aaatagggat gcaactcgac
     7141 ctggttgacc atcggtttaa ctcttaccgt ttcacaaaag ttcgccagaa catgaggata
     7201 gaaattggaa accccaattg cttttaattt gccctcttca taggcatctt cgagtgcacg
     7261 ccacacactg aaataatcgc gcatagcctg gtgcaataaa tagaggtcga agtactctag
     7321 tcccgatttt tttaatgatg cttcaatacc tgctgctgcc gtatcctgat tcaacatatc
     7381 ctgcccccac aatttagacg ttataaataa ctcatctcgg gtacataaac cttcagatat
     7441 agcttcacga acggcttcac cgacggcatc ttcattaccg tataccgctg cagtgtcgat
     7501 gagtcgataa cccgtgcgga tagcgttcaa cactgactgt ttgcacacgt ttttatcggt
     7561 aacctgaaaa acaccaaatc ccatcatcgg cattttcaga ttgttactta atacggaaaa
     7621 ttccactgtc agtccctcca ttaggcatga acaatgagtc tacgttaaaa cataacctca
     7681 aattagtatg tggattttga tatcacttat gcaaaaaatt cattaataat gtaggactga
     7741 aacctctcta ttttcgggga caacgaagca gacgctacca gtgcttttgc cttcgacctt
     7801 gctgtttttg atacacttag ggccttgggc ataacgaaaa tgtgcgatat cacgaagaat
     7861 taatggagaa tgt
//
LOCUS       DAJVYM010000164         7825 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVYM010000164 DAJVYM010000000
VERSION     DAJVYM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.166"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OQS60_003432"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OQS60_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS2389107.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OQS60_003433"
     CDS             complement(2975..3697)
                     /locus_tag="OQS60_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS2389108.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OQS60_003434"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OQS60_003434"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS2389109.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>7825
                     /locus_tag="OQS60_003435"
     CDS             6679..>7825
                     /locus_tag="OQS60_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCS2389110.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEG"
BASE COUNT         1885 a         1935 c         2234 g         1771 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtg
//
LOCUS       DAJVYM010000165         7818 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVYM010000165 DAJVYM010000000
VERSION     DAJVYM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.167"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..921
                     /locus_tag="OQS60_003436"
     CDS             <1..921
                     /locus_tag="OQS60_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2389111.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="OQS60_003437"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="OQS60_003437"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS2389112.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="OQS60_003438"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="OQS60_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS2389113.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="OQS60_003439"
     CDS             3882..4277
                     /locus_tag="OQS60_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS2389114.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="OQS60_003440"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="OQS60_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS2389115.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="yraP"
                     /locus_tag="OQS60_003441"
     CDS             4897..5472
                     /gene="yraP"
                     /locus_tag="OQS60_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS2389116.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="OQS60_003442"
     CDS             complement(5586..6626)
                     /locus_tag="OQS60_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2389117.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="OQS60_003443"
     CDS             complement(6699..7334)
                     /locus_tag="OQS60_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS2389118.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..>7818
                     /gene="yhbO"
                     /locus_tag="OQS60_003444"
     CDS             7462..>7818
                     /gene="yhbO"
                     /locus_tag="OQS60_003444"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS2389119.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGR"
BASE COUNT         1945 a         2134 c         2015 g         1724 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgc
//
LOCUS       DAJVYM010000166         7802 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVYM010000166 DAJVYM010000000
VERSION     DAJVYM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.168"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1340
                     /gene="yjeM"
                     /locus_tag="OQS60_003445"
     CDS             <1..1340
                     /gene="yjeM"
                     /locus_tag="OQS60_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCS2389120.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1491..4814)
                     /gene="mscM"
                     /locus_tag="OQS60_003446"
     CDS             complement(1491..4814)
                     /gene="mscM"
                     /locus_tag="OQS60_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS2389121.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4836..5804)
                     /gene="psd"
                     /locus_tag="OQS60_003447"
     CDS             complement(4836..5804)
                     /gene="psd"
                     /locus_tag="OQS60_003447"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS2389122.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5901..6953)
                     /gene="rsgA"
                     /locus_tag="OQS60_003448"
     CDS             complement(5901..6953)
                     /gene="rsgA"
                     /locus_tag="OQS60_003448"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS2389123.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7048..7593
                     /gene="orn"
                     /locus_tag="OQS60_003449"
     CDS             7048..7593
                     /gene="orn"
                     /locus_tag="OQS60_003449"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS2389124.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1782 a         2028 c         2096 g         1896 t
ORIGIN      
        1 taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggtggtatc tattcctgga
       61 tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg ttttcctctt
      121 atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca acattcctct
      181 atggtagcga catgacccag cactggcgta ttgccggact ggagcctacg caggtggttg
      241 gtctgctggc agtagcatgg atgattctgg tcaccgtcgt tgcttcaaag gggattaata
      301 aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat ttagtattgc
      361 tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag gatattaatt
      421 tccttgcatc accgaacccg ggttatcagt ccggtctggc aatgctatcg tttgtggtat
      481 ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat aaaacggaaa
      541 atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt tcaatcggtt
      601 attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta ttaagtaatg
      661 gttccgttaa ccttggcaat attacctatg tgctgatgaa gagcctcggg gtgacgctgg
      721 gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg tttgcgcgta
      781 ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt ctttacgctt tgctattcac
      841 cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg atgacgcgcc
      901 tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgcgggttg gttactgtct
      961 tcatcctgct ggtttcgttt ggtggcggta ccgcatccgc gttctttaac aagctgacgc
     1021 tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct ttcccattct
     1081 ttaaagcacg tcaggatctc gacagaccat ttgtgatttt caaaacgcgt atgtcggcaa
     1141 tgattgcgat ggtggttgtc gtactggtgg tgacatttgc gaacgtcttc accattattc
     1201 agcctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc ggccctgtct
     1261 tcttctcgct gttagcgatg gcgatttacc agaactattg cagtcgcatg gcgaataaac
     1321 ctgagttagc tctcgactga tcatatgaaa tcctgcaact catcgtctct ccggcgatga
     1381 gttgtcagga agtaatgcgt atggatgata ctctccaggg ccggtgataa ctgaagatga
     1441 tgttaagctg ctaaatgagc cacgttgaca tcatttggca gcttcatgca ttacaaactt
     1501 cccgcctgac gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg
     1561 ctttccagac gcatctggaa gggcgggaat ggcatatcga taccgtgggc atggaagcca
     1621 gccagaatca gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggca
     1681 taaatacgca gctcgaaaat ctgaatccct tgttgcagat ccaccaggaa gacttccggt
     1741 gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact
     1801 tcttcactgt tggcatcagc aggggccgga atcgtcaaca ctacgcgcgt gaccgagtca
     1861 gagagcgacc agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct
     1921 ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggtg
     1981 agatcgcgaa ttgtcaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcaga
     2041 ccagagataa agttggcgaa aatttcctgc aaaccaaaac cgagaccaac accgagcgcg
     2101 gcgaccagcc actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg
     2161 ccaatcagca tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt
     2221 aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc
     2281 gtggtgatga taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga
     2341 ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg
     2401 aaagcggaat ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa
     2461 attgagcgca ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca
     2521 tcgacttcaa ttgctccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgca
     2581 cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcggcgc
     2641 tggatcagca tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca
     2701 accgaggttt caagcctcgc taacagcgcc tgtgccgttg ccagataacc cacagccgac
     2761 gccagaatgg caaccaacgg cgcgccaatc atcatgttcc acagcatatg gttggtaatg
     2821 ttgtcgccgc tgccttcttt gttgagatac agcggaatcc cggccttttt caggctgagg
     2881 gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa gccttcccag cgaaccggag
     2941 aattcacggt cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc
     3001 ccgatgctca tcaggtagta gcgcatccca cgagatacgc gttcgcgcgg ccagccaaaa
     3061 tgagcgataa acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc
     3121 agcggcacgg tggccgttac accatcgcca atcgcgaccg ccagcggata aggccacgcc
     3181 tcgcgcaagc cgtaacccag cgtcatccac agcaccggta acggtgacgc gacgagaatc
     3241 gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccaactttc
     3301 gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc
     3361 agaatcaacg cgccaaacag cggcaaaatt gtctctttgc tggtcagcat catcacactg
     3421 gctttgccca actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt
     3481 tccagcggcc aggcgatggt catcggacgc acgtcagagg tccagaacag atagcggtgc
     3541 gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc cgttggagac tttcagcttg
     3601 gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga
     3661 gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc
     3721 tgatggattt gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc
     3781 acacgcaact gcgccatttc ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt
     3841 gccacctgcg ctcgcaacgc ttcaccgagc agattggacg atcccagcca ttgcgactgt
     3901 tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt
     3961 tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg
     4021 ttaattttga attgcgcgac gatatctttc ggcaaatcgg cactgttttc tgccagtaat
     4081 tcggtacttt ccagcgcccg ctccgcctca agctgacgtt ggctattcaa ctgattacgt
     4141 aaggcctgca aatacgcatc cagttgctgg ctctcttttt ccgcaagctc tgagcgtaag
     4201 cgcgctaatt cttggcggtt attggcagac agctgcgcca gctccagttc atcaacgagc
     4261 gccttaagac gtgcagagtc agactgcaac gcgaaatttt gtgcctgatt gagcggagta
     4321 ttgccggtaa gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt
     4381 tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc
     4441 tgggcctgac ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc
     4501 gcgtcggtag acattcccgg cgacacgctg cgcggcttgt cacgcatgtt gtttaattgt
     4561 gcgcgcagag tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg
     4621 cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg cagactgaag cgcctctacg
     4681 acttccggct gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg
     4741 ctatcggggg ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg
     4801 ataatcaggc gcacgtcagc gtttcctttg atggattaga cctggtcttt tttgtcgtca
     4861 accaatgggc tggcgtcgtg ttctgcttcg atctcttcag caggaagcgg ggcaggttca
     4921 gcgtctggcg taacaaaggt ttcggtagat actgccagcg gctggcaaat tttcgtgaca
     4981 gacaggcttt ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg
     5041 gtggaaccga gtttaaagcg acccatttcc tggcctttca gcagtgccac agaaccgtcg
     5101 ttttccccgg caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg
     5161 cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt
     5221 gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc
     5281 acgttctgag ccgtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca
     5341 cgcagaatac cgttgcacgg catgtgtacg cggtggtagt cacgcgggga gaggtaagtg
     5401 gtcacaaacg taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct
     5461 tcgaggctgt agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac
     5521 tggctgataa cgccatcggc aggcatgacc agtacattcg gatcggtatc gattgggcgt
     5581 acttcgtcac gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc
     5641 ggcttttgcg cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt
     5701 tttgtcaacc atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt
     5761 ttcggcagaa tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca
     5821 ttgttttgtc gttcctgatt cggcctacat gccggatcct gaaaaaaagg ggacgattct
     5881 aacgacggtt agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc
     5941 tgcgccatgc tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct
     6001 tcctcaaccg cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat
     6061 ttacacagac ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc
     6121 aggtgccaga ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg
     6181 aagtgataca gccgagcggc ggtcgtggtg tgctgaccaa gccccgagtt gtcagagaca
     6241 tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt tcagcaggct ggatttgccg
     6301 acgccagact gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt
     6361 agcccatcct gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata
     6421 tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc
     6481 acaataatcg gctcaatctg caaggtttcg caggccacta ggtaacggtc gataatattg
     6541 agcgacagct ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata
     6601 ggttttacgc cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct
     6661 tccacgatcc ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg
     6721 cggtcgccgg ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca
     6781 tcggcggatt ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc
     6841 tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg
     6901 tgattggcgt tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtact
     6961 gactcctggt cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat
     7021 agtcactgtg aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc
     7081 gatcttgaga tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg
     7141 gtgaccgatg ccaatctgaa tattctggca gaagggccga ccattgcagt acatcagtcc
     7201 gatgaacagc tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg
     7261 gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa
     7321 ttcttaaaac agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggacag
     7381 gaccgtcgtt tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat
     7441 ctcgatgtca gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt
     7501 tttaccaagc aggggacgca tcaggcaatg gatgatatcc gtgaatcggt ggcggagctg
     7561 gcttactacc gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg
     7621 aatccacttg cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc
     7681 acttgagata aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg
     7741 tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag
     7801 ta
//
LOCUS       DAJVYM010000167         7715 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVYM010000167 DAJVYM010000000
VERSION     DAJVYM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.169"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..643
                     /locus_tag="OQS60_003450"
     CDS             <1..643
                     /locus_tag="OQS60_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2389125.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="OQS60_003451"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="OQS60_003451"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS2389126.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="OQS60_003452"
     CDS             2474..2986
                     /locus_tag="OQS60_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS2389127.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="OQS60_003453"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="OQS60_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS2389128.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="OQS60_003454"
     CDS             5579..5782
                     /locus_tag="OQS60_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS2389129.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="OQS60_003455"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="OQS60_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS2389130.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="OQS60_003456"
     CDS             complement(6827..>7715)
                     /locus_tag="OQS60_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS2389131.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1784 a         2101 c         2021 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAJVYM010000168         7704 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVYM010000168 DAJVYM010000000
VERSION     DAJVYM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.170"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            75..1463
                     /locus_tag="OQS60_003457"
     CDS             75..1463
                     /locus_tag="OQS60_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS2389132.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="OQS60_003458"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="OQS60_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS2389133.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="OQS60_003459"
     CDS             complement(2010..2114)
                     /locus_tag="OQS60_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389134.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="OQS60_003460"
     CDS             2284..2730
                     /locus_tag="OQS60_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS2389135.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OQS60_003461"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OQS60_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS2389136.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="OQS60_003462"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="OQS60_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS2389137.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="OQS60_003463"
     CDS             4841..5188
                     /locus_tag="OQS60_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS2389138.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OQS60_003464"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OQS60_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS2389139.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="OQS60_003465"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="OQS60_003465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCS2389140.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="OQS60_003466"
     CDS             6705..6803
                     /locus_tag="OQS60_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS2389141.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="OQS60_003467"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="OQS60_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS2389142.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="OQS60_003468"
     CDS             complement(6939..7187)
                     /locus_tag="OQS60_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS2389143.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="OQS60_003469"
     CDS             complement(7335..7517)
                     /locus_tag="OQS60_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCS2389144.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..>7704)
                     /gene="yeaR"
                     /locus_tag="OQS60_003470"
     CDS             complement(7521..>7704)
                     /gene="yeaR"
                     /locus_tag="OQS60_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCS2389145.1"
                     /translation="SAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAPE
                     VLMEGAQQRKVIHNGK"
BASE COUNT         1898 a         1884 c         1836 g         2086 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc ataaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacact atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actg
//
LOCUS       DAJVYM010000169         7637 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVYM010000169 DAJVYM010000000
VERSION     DAJVYM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.171"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            353..1306
                     /locus_tag="OQS60_003471"
     CDS             353..1306
                     /locus_tag="OQS60_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS2389146.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1306..1602
                     /locus_tag="OQS60_003472"
     CDS             1306..1602
                     /locus_tag="OQS60_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389147.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            1926..2216
                     /locus_tag="OQS60_003473"
     CDS             1926..2216
                     /locus_tag="OQS60_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389148.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2437..2652
                     /locus_tag="OQS60_003474"
     CDS             2437..2652
                     /locus_tag="OQS60_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389149.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            2799..3104
                     /locus_tag="OQS60_003475"
     CDS             2799..3104
                     /locus_tag="OQS60_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389150.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3569..4279
                     /locus_tag="OQS60_003476"
     CDS             3569..4279
                     /locus_tag="OQS60_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2389151.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4304..4510
                     /locus_tag="OQS60_003477"
     CDS             4304..4510
                     /locus_tag="OQS60_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389152.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4609..6789
                     /locus_tag="OQS60_003478"
     CDS             4609..6789
                     /locus_tag="OQS60_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS2389153.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            6946..7534
                     /locus_tag="OQS60_003479"
                     /pseudo
     CDS             6946..7534
                     /locus_tag="OQS60_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2524 a         1347 c         1639 g         2127 t
ORIGIN      
        1 aggtgattca gatattaaga agtatccctg tgaacaataa atgaccaatc agtaattatt
       61 ctgtgaaata attgttgcaa tttataatca catctgttat agtttttata tggggtgata
      121 aaagaactca gaacattaag aggttacaac gttgggaggc gtcagggtat tgacagattt
      181 tcacggcctc ttattcaatc aaggcttttc cttttgctgt taatactcgc gtaatcgctc
      241 aggcttctga gttcctttat cagtccatct tctaattaat tcactccctc tggggttggt
      301 cactatacgt ggtcagccct ttctttgttt tatttttaaa aaggaaaata taatggaaaa
      361 attccacatg tttgatgacc gtatttatca gatgactaat ggcgttaaag ctgccgaccg
      421 tattatttac actgtcatgt tgaatgaaca aaagttttat cacgataagg ggcaatatta
      481 taccccttca tatgctcatc ttggtcagta tgctggaatc acaagaacat ccgtgattgc
      541 atgtatgaaa caacttgagg cacatggttt aatcaagaaa actaacgttg caagcaatca
      601 gtgtgttatt aaagtttatt cacttgatga agtaccggag gttttaacag agcttacagc
      661 taaggaagcc agaaaggcaa agctggagtc taaagttact cagtcagtca agaaagatga
      721 cccactgagt caaaattctt tacccactga gtcaaaattt tttactcagt cagtcaaaat
      781 ctttgacacg tataataaca gtaataacaa gaaagaactt attaataaca ataaagaaga
      841 tgactttgaa gaagaatatt ttaagaaggg agcttcgctc tgtgattcac atgaatgtga
      901 ttctcttggt attgaagagg ctattaaaga gactgttaaa acatctgact tagatgacct
      961 tgatagtgaa gctgctaaag aggtcgttaa aaaggaagat aaaatctctg atgtgattgc
     1021 tagtagtgac cataaaaaca cacagtgccc cgccactgat gatgaattac cggatgttga
     1081 ccttgatgat gaagaagacc ctgaaaagga attgaaacgt gaacagcgta aagcagaaat
     1141 taaagaaaga ttcagaaatg cgaagctaaa gaacaaggtt agtgacctcg atgactttga
     1201 tgatagtgac gatgatttta cttttacacc aaaaaagaaa actcacaccg agccaaagaa
     1261 agaaaaagtt aaagtcagac gtgtttatgg tggaggaatg ttttaatgaa aacttataat
     1321 ggcgagagaa aataccgtga ctatttactc agacgttaca gcatttctcg tgaaggttac
     1381 ttgatgaaag atactcacgg ggaagtttac cggattcgac cgaagaaaga agggagggat
     1441 tattatttcg tcgatgatgt gacagattta aagattgacg ctttgaagtt tgcggtgctg
     1501 tatcattacg atttatggga ttcagtccac cagctacgat taaaagacgg tgaccccggt
     1561 aatctgaggg ccaccaatat tatcaaggga aagtgccggt aaggccacca gctatttatt
     1621 tactgaaatc tctatctcaa cacgaagaag tttaagtcga attgtaagct ttgtcttttt
     1681 tgtaatgact cgattcataa cgcaaaacac caatagcaca ataaataccg caaagcccaa
     1741 cgagctaaaa cgatgagatt caataattgc tgtgaaaaaa tcatgcatta attttccttt
     1801 tgtgaaagga aacagcacct gtatcagccc ctacaaccat agtatcatgt aaatgaggta
     1861 ctgacaatat gttaaataga tgttaagatt gtagtatgag taagagattt aaggagattt
     1921 ttacaatgac tcacacagtt acgcgccttt tgccagaggc atttgaacga ctggcagcaa
     1981 tcaaagccaa atatcccgat atgaagcaca cagcgcttgt tggggagatt ttaaaagagt
     2041 cggtaaacct tagccctttc gataacgtta agggcgtcgc agagggttct aaggccatca
     2101 gaatcacaac gggccaactg gaactactca aagcacagtc cgcagtagca ggttgctcaa
     2161 tgattcaggt gttaagcgct aaggtccatc aactggcgga tagattggag gtttaatgtg
     2221 ttataggttc caatattcca cacttggccg ccacctaatc agacagaatt tagcagatgc
     2281 tgaatttgaa gatgtgaccc tcaaagcaaa caaacgatta aaaccctctc aaatccacca
     2341 gcttaaacac gaattaaagc cactggtaat actcgactat aacgaccctg aacagcttga
     2401 gcagggaatt aagcttattt ctgaatgggg tgcttgatga aaactatcac agtacaactt
     2461 cagactaata aagcatttcg ttattttgaa aacttgcttg agttatacga aggatgggga
     2521 agtatccacg gcaaagatga tatctatctt cacttgtcag cccctaacta ttctctgaag
     2581 actccggtta agcaaagctg gttaaaagac tacggtcacc agatggggct attagtctca
     2641 gacttgagct aaaaacagca aaacatatct ctcaggcttt accagacgca ccagaaacgt
     2701 ttttaagccc ctaatcaggg taacttaggg aatcgcctta agtggtctaa aaaaggggtt
     2761 aaaacgtctt agaatgcgat acaggaagga ttttaaaaat gaattaccca atgcaaatta
     2821 tggttgacca agaaaccatg aattggttga ttgacctgaa caggtcgggc tttaatatct
     2881 atttcatgca ttgttttgaa gcgtcaacgt atattgacaa gcatataatc aacgaaatta
     2941 aacttggtga ctatgttcac aagctggaag taacagaccc atacaataag cgctttatca
     3001 ttgaagagtg caatcgactt gatttaagcc ctgagcaatt attgaagctg aatgtattct
     3061 tgacctatca gaatgaatta aacccttaca gcgaggtgat gtaatgacac tcgttattct
     3121 ctggatgact ttagggttat ttgttggttg ctgggtaggt aatacggtaa ataagaattg
     3181 atgttaccaa tctgttaaaa agctgttgac aaataacctt aagcatgaga tgatggtaat
     3241 agggaagagc aactcttcct gaattaaact attaatcatt ctacatcaat gatagttatt
     3301 ctgaaaggtg caatgctttt cgctacatga cctttcaggt tagtctgaga ggtcatctcg
     3361 aaaagtactc ttgttcattc gtttgaatcc tttagatgtt ttaatttctt caccttatga
     3421 atggcccttt ataagaaatt atctagggct attcagcttt taaaaagcat acttggcaaa
     3481 ccgcctttta agtctcttct aagtttagat aggctgtcag gtgtgctttt taaaaactga
     3541 aacacttccg cttagaagag gaaaagcaat gacaaatgat atttacatta atggcaaagt
     3601 gattgatagc ttcagtgact ggtcgccaca gactgaacat ccagctatca ctattcagcg
     3661 taaagagcat gacaagcgta ttgagcttga gcaatcgcta aagatgtcac caaaaacaac
     3721 catctttgtg tctcctgagc ctcaagagat gcctgatatt tgcaagagtg aagcattact
     3781 tgagcttgaa aagaaatatt accaactgct gaaagaacag cgccttagat tagatgacgc
     3841 ttacagcaaa gttaccttga tgcaatcagc tattgagccg tcagaatttg agattcagga
     3901 tgaattgtca cagaaaccat ttgtctatta ccaatacgaa gataatgatg gttttggtac
     3961 ttttcaagaa aacgtcgcag ccgtgattag tagtcttcct gctggacatc gaattgtgaa
     4021 gatggttaag gcatcaagag gtgctggtca gtttatttac atgactaata agagcgatga
     4081 agaactcgct gaaattgcta gacagaacat cttggcatca cgtaacaagc aacttgataa
     4141 tgttaagttg tatctctcaa gagagcttca ggctatgaaa gagcttgtga aagaatatga
     4201 agatgctaag aagattgcta tgcaggctga tattgagcag cttacgaaga tttctagcaa
     4261 atatgcaaag gctatttaat taataattaa aaggtggtgg tgaatgattg atttaacaga
     4321 acgctatgag cttattaaag caattagtga acgattgaaa gaacaaccca acaaaccaaa
     4381 acgaatcaaa tcattcacct cctctaagca aaagaataga atcaggaaaa tagctaagtg
     4441 gtgtcttgat aaattaccaa gtgattgtca gattgttggt gaagatggta attattatct
     4501 ggttaggtaa caagattggt tgggtgaaaa tctacgggag agattatcga gtgatatcgg
     4561 tagtctttcc ctttttcgta tatgaacact taaagaggtg aaacatgaat gagcaaattc
     4621 acagttgata gttttatcgt tgaacttagt ttcagtgaaa acgttattaa gggcttgcaa
     4681 agagttgaaa aggcagcttt gcagtcagca caaagaattg aacgcaattt gaatagagcg
     4741 tttaaggtcg ataccaagca acttgacagc aatctgacaa gttctttagg gcagcttgaa
     4801 aggaagttta ataagacctt tgacaagatt gagcagagag caagaagcac aaaagcattt
     4861 cagtttgcca ccagatttaa tgacactgtt aatcctccaa aacaatcaag gcaaccacga
     4921 attagcggaa acagagcaat cacagcagcg catagcgtga atatgtctaa gcttggtgat
     4981 attaatccgc tgatggctaa aatgtttaaa gcacagtatt acagcttaag cggtaaggct
     5041 ggtaatattg gaagtgaaaa gtttaacgct gaacttgcaa aattaaatca atctcttcgt
     5101 gaaacgttga ataaactcag gtcacagaca aagaccgcca gcattaacaa tacaagcgac
     5161 gcatttaata atcttgcaag caatgcgatt aaattatctg gtgcatttta ctctgtgatg
     5221 ggtgctctta acgcttacaa ggccattatg aatgcaggtc ttaaacgaga ttccgcacag
     5281 agagccgcta agtttgtact tggtgataaa gcatctgaag ctgaggtatt tattcgaaat
     5341 ttagcagaca agacaggtct taatatctct gaagggctat caagctacgc taagtttgca
     5401 gcaggtgctc agggttctat gtcacaggaa cagacacagg agctatttgg taatgcaacg
     5461 gctatgagtc gcttaatggg gctttctaac gatgagctaa acggcatttt gagagcattt
     5521 gagcagatgg cgagcaaggg caagattcag gctgaggaat tacgtgggca gttaggtgac
     5581 cgtatggctg gtgcatttaa gctattcgct gaagcattgg gtatgactgc tacagagctt
     5641 gatgcagcca tgaaaaacgg gaaggtactt tccgctgata ctctccctaa agtcgctaaa
     5701 cagatgggct tgatgattga taaagcgggt ggctgggctg aagtcactaa gagcactcag
     5761 acagctttag gtaagttagc aaacaattgg gatgatacga tggtgaaaat cttctcaggt
     5821 tcacaggatg agctaaacgg cttcttatcc agcctgagta acctgcttag tgaaatgggg
     5881 atgagttctt ctatcgctgg tgatgctatc ggtggactta tcgacatgct taaagctggt
     5941 gtagatgata ttcgcgtatt caataacacc attgaaggct ggattttaca gactaagaag
     6001 ttctattaca gccttgatga cactaaacga aaactacttg atgaagtcgg tgatggtttt
     6061 atcaacttcg tcaaaggtct tgctatagcg ttatctgcaa agacgctatt cagtgctact
     6121 acaggggtta tgaacctgac aagagcaatc acaacgttag gtacaagagc taatcaggtg
     6181 gctggtcagg tagctactgg taaaggtggt ggaaaactga aaggtgtagc tggtggtgta
     6241 ggtagtgctc ttgcaattgg ttatgctgat gacggttatg aaacagcgtt agcattagct
     6301 tcaatgattc cacaaatcag aggcgtcact cttggtttat atgcattgaa gaaagcactg
     6361 gattttatga atcaggaagt agtcaaaaac gctaatatgc atccgtctgg ggtaggtgtg
     6421 ggtagtgact ttaatcccgt gtattctggt gaccctaata aaccgaatat gattaacgaa
     6481 ggatggagta gaatttttag ttcaatgggt gaattgttta acaatgccac aatgaatatt
     6541 gcaagattca atcatccaga gttaaacaac attaagcaag attcagcatt aacgagtgca
     6601 gatattcagg gtctgaggga tgaaattagt gctttgagta agcgtattca ggagccagtg
     6661 aaagtttcac ttggtggtca agttgtcgtt cgaccagatg aaccatcttt ctttactttc
     6721 aatcaatcag ctaatgacca gttcacgcag gatatgctct taagttcaag ctatccagag
     6781 gaagaatgat ttaacggtgt tctacgtgaa taaagcaaag ttaataccca caccaaaaat
     6841 tatttttcac gtagctcacg cttaacagag aaaatacata tatatcaaag gcttggttaa
     6901 aaatgattaa aatacactaa gcctttgaaa tcaaaggaga atttaatgat ttacactaat
     6961 cagcttcgtt acagtggcaa agatggcatc tatttccact taagagacaa tgtagatgcc
     7021 ttcttaacct tatcagcaac tgaaaacatg gaatttgata gccctatgca ggtgactaca
     7081 cagaacatgc aattatgaag gaagcttatt attaactcgt cagggtcaat tagtagaaaa
     7141 cttcattgac actctggaaa actggcgtga ccagaagcag attatttctg ttatctgtaa
     7201 agatggaatt aaaattgacg attccattat taccaacttt aaagcctcta aagatgttac
     7261 tatctctaac ggtcttcgta tccagctaac ttttcaggaa attaacttta aagcaatcgt
     7321 aggtcaaact gatatttcag cagccactgg caaaacggct accacaaatg acggtggtgt
     7381 gaccagtaag aagaatgcag gtaacgtgac cactgaacgc aataacgcgc ctatgctatc
     7441 gtgcaaagag ttaatgaata ttagcgatgt atccggttac gctccagcta tcagaaatga
     7501 tattctcgat gctcagatta aatgtaataa ggcttggaag ctggactatt cagacttaac
     7561 agaagctgaa aaaaccaacg tcaccaatgc taatagtaac gctggtcgag tagcttcaaa
     7621 gcttaaaaag tactcag
//
LOCUS       DAJVYM010000170         7623 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVYM010000170 DAJVYM010000000
VERSION     DAJVYM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.172"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..96
                     /locus_tag="OQS60_003480"
     CDS             <1..96
                     /locus_tag="OQS60_003480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2389154.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OQS60_003481"
     CDS             complement(152..487)
                     /locus_tag="OQS60_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS2389155.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OQS60_003482"
     CDS             complement(491..835)
                     /locus_tag="OQS60_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS2389156.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OQS60_003483"
     CDS             complement(837..1010)
                     /locus_tag="OQS60_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389157.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OQS60_003484"
     CDS             1111..1296
                     /locus_tag="OQS60_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389158.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OQS60_003485"
     CDS             1257..1376
                     /locus_tag="OQS60_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS2389159.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OQS60_003486"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OQS60_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS2389160.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OQS60_003487"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OQS60_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS2389161.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OQS60_003488"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OQS60_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS2389162.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OQS60_003489"
     CDS             4176..4544
                     /locus_tag="OQS60_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCS2389163.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OQS60_003490"
     CDS             complement(4652..5053)
                     /locus_tag="OQS60_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS2389164.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OQS60_003491"
     CDS             5295..5588
                     /locus_tag="OQS60_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS2389165.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OQS60_003492"
     CDS             5660..6319
                     /locus_tag="OQS60_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS2389166.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OQS60_003493"
     CDS             6411..6857
                     /locus_tag="OQS60_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS2389167.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OQS60_003494"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OQS60_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS2389168.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAJVYM010000171         7603 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVYM010000171 DAJVYM010000000
VERSION     DAJVYM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.173"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(73..2451)
                     /gene="ppsA"
                     /locus_tag="OQS60_003495"
     CDS             complement(73..2451)
                     /gene="ppsA"
                     /locus_tag="OQS60_003495"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS2389169.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            2784..3617
                     /gene="ppsR"
                     /locus_tag="OQS60_003496"
     CDS             2784..3617
                     /gene="ppsR"
                     /locus_tag="OQS60_003496"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS2389170.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3774..4820
                     /gene="aroH"
                     /locus_tag="OQS60_003497"
     CDS             3774..4820
                     /gene="aroH"
                     /locus_tag="OQS60_003497"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS2389171.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            4952..5143
                     /gene="hemP"
                     /locus_tag="OQS60_003498"
     CDS             4952..5143
                     /gene="hemP"
                     /locus_tag="OQS60_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS2389172.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5147..6583)
                     /locus_tag="OQS60_003499"
     CDS             complement(5147..6583)
                     /locus_tag="OQS60_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS2389173.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6646..7359)
                     /gene="ydiV"
                     /locus_tag="OQS60_003500"
     CDS             complement(6646..7359)
                     /gene="ydiV"
                     /locus_tag="OQS60_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS2389174.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1928 a         2022 c         1805 g         1848 t
ORIGIN      
        1 tgaagagatt gaataagttt catcttcggg gatcacataa ccccggcgac tgaacgccgc
       61 cggggattta ttttatttct tcagttcagc caagcttaac caggtttgca ccacggtgtc
      121 cgggttcaga gacaggctat cgatcccctc ttccatcaac catgcggcaa agtcttcgtg
      181 gtcggacgga ccctgaccgc aaatcccgac atatttgccc tgtttcttcg cggcacggat
      241 agccatcgac agcagtgctt tcaccgcatc gttgcgctca tcgaacaatt cagacaccac
      301 gccggagtca cggtccagac cgagcgccag ctgcgtcata tcgtttgagc caattgagaa
      361 gccgtcgaaa tattcgagga actgctcggc cagcaaggcg ttggacggga tttcacacat
      421 catgatgatt ttcagcccgt tctcaccacg cttcagcccc tgacgcgcca gttcctcaac
      481 caccgctttc gcctgatcta cggtacgcac gaacgggatc atgatctcaa cgttggtcag
      541 ccccatgtcg ttgcgcacac gtttcactgc ttcgcactcc agcgcgaagc agtcgcggaa
      601 gctgtcggaa acatagcgtc ccgcgccacg gaagccgagc atcgggttct cttcatctgg
      661 ctcgtaacgc tcaccaccga ccaggttggc atattcgttc gatttaaaat cagagagacg
      721 gacaatgacg cgcttcggat aaaacgcggc acccagcgtc gcgatccctt cagtcagacg
      781 accaacgtaa aattcacgcg gagaatcaaa acctttcatc atctcgcgga tttcattttg
      841 caactgcggt tcctgatcgt caaactcaag cagtgcgcgt gggtggacgc caatcatacg
      901 gttgatgata aattccagac gcgcaaggcc cacgccttcg ttcggcaggc aggcgaagtc
      961 gaaagcacgg tccgggttac cgacgttcat catcactttc aacggcagat ccggcatcat
     1021 ttctacgctg gagcttttca cgctaaattc cagcaactcc gcatagacgt aaccggtatc
     1081 accttcggca caagaaacag tgacgttctc accgtctttc atccgttctg ttgcatcacc
     1141 acagcccact accgccggaa tgcccagttc acgagcgatg atcgccgcgt gacaggtacg
     1201 accgccacgg ttggtgacga tggcagatgc tttcttcatg atcggttccc agtccgggtc
     1261 ggtcatgtca gtgaccagca cgtcaccagg ttcgatgcgg ttcatttcgc tgatctcatg
     1321 gatgactttc accggacccg cgccgatgcg atgaccgata gcacggcctt cggcgataat
     1381 cttaccctgt gaatgcagcg tataacgctc catgacctga ccgcgtgagc gcacggtttc
     1441 cggacgcgcc tgcacaatga acagtttgcc agtgtggcca tctttcgccc actcaatatc
     1501 catcgggcga ccgtagtgtt tctcaatttg tacggcctgt tttgccagtt cctgcacttc
     1561 ttcgttggtc agcgagaaga tgtcacgctg ttcctgcggt acgtcttcga ttttaacctg
     1621 cttgccgtgc tcctgggtcg gcgcgtaaac catgcggatt tttttcgacc ccatggtgcg
     1681 acgcacgata gccgggcgat tcgccgccag tgtcggttta tggacgtaaa actcatccgg
     1741 gttaaccgca ccctgcacga ccatttcccc aaggccccat gcggaagtga taaacaccac
     1801 ctggtcaaag ccggattcgg tatcaatgga gaacatcacg ccagaagatg cgaggtcgga
     1861 gcgcaccatc cgctgaacac cggcagagag cgccacgcca cggtgatcgt aaccctggtg
     1921 cacacgataa gagatggcgc gatcgttaaa cagagaagca aatacatgct tcactgccac
     1981 gagaacggcg tcaaaaccct gaacgttgag gaaggtttcc tgctgaccgg caaaagaagc
     2041 gtccggcata tcttctgcgg tggcggagga gcgcaccgca aaagaggcgt tttcgtcatc
     2101 ggcggaaagc tgtgcatagg cttcgcggat ggcgttttcc agctcaggct ggaagggagt
     2161 gtcgataatc cactggcgga tttgcgcgcc cgctttcgca agctgagtaa catcgtcaat
     2221 atccgtttta tccagcagtt cataaatgcg ctggtttacg ccgctttggt ccagaaactg
     2281 gttaaacgcg tcggcggttg tggcgaaacc attcggaacg gaaacaccca ttccggaaag
     2341 gttagtaatc atttcaccca gggaggcatt tttgccccca accctgtcta catcattcat
     2401 gccgagttgg ttataccaaa gcaccagcgg tgacgagcca ttgttggaca tcgaacaatc
     2461 cttttgtgat aaatgaacgg tttgagaaac acatttctgc gcatttatct ttgcatattt
     2521 aaccggatga aaaaaacggt gaatcgttca agcaaatata tttttttact ttttaagact
     2581 gatcccagcg ttgcgcaaat ctgctctctt cgacaatttc cacataaacc atcggtataa
     2641 acgaaaccat aaaaatgaaa tgctgttttc ataaaaaata aaattgaagg ttcattttat
     2701 aaaccagaca taacgtccta cgcttctgtg cgtttttaat ttatgctttc atagaattat
     2761 gtctgcatca cgggaagaac aaaatggata atgctgttga tcgccacgtt ttttatattt
     2821 ctgatggtac ggcaataact gcggaggtat taggacacgc agtaatgtca caatttcccg
     2881 tcactatcag cagcattacg ctgccgtttg tcgaaaatga gagccgtgca cgggcagtga
     2941 aggatcagat tgacgcaatt tatcaccaga caggtgtgcg cccgctggtc ttctactcca
     3001 tcgtgttgcc ggagattcgc gccatcatct tgcaaagtga aggcttttgc caggatatcg
     3061 ttcaggcgct ggttgccccg ctacaacaag agatgaaact ggatccaacg ccgattgctc
     3121 atcgtaccca tggccttaac cctaataatc tcaataaata tgatgcgcgc attgcggcga
     3181 ttgattacac cctcgcccac gatgacggca tttcgttgcg caatctggat caggctcagg
     3241 tgatcctgct cggtgtttct cgctgtggta aaacccccac cagtctttat ctggcaatgc
     3301 aatttggtat ccgcgcggca aactacccct ttattgccga cgatatggat aatctggtgc
     3361 tacccgcgtc gctcaaaccg cttcagcata aattgttcgg cctgactatc gacccggaac
     3421 gtctggcggc gattcgcgag gaacgtcggg agaacagtcg ctatgcctcg cttcgtcagt
     3481 gcaggatgga agtcgcggaa gtggaagcgt tgtaccgtaa aaatcagatc ccgtggatta
     3541 acagtaccaa ttattcggta gaagagattg ccaccaagat cctcgatatc atgggcctta
     3601 gtcgccgaat gtactagaga actagtgcat tagcttattt ttttgttatg atgctaacca
     3661 cccggcgagg tgtgacacac ctcgcacttg aaatcagcag cgattggttt atcgtgatgc
     3721 gcatcacttc ccggcagtcc tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca
     3781 gaactgacga actccgtact gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc
     3841 tacggtatcc cgtaacgcct ggcgccgcca cccatgtcac cgactcccgc cgcagaattg
     3901 aaaaaatact caatggtgaa gataagcgac tgttggtcat tattggcccc tgctcgatcc
     3961 acgatctcac cgctgcaatg gagtacgccc cccgtctgca gtcgctgcgc aaccagtacc
     4021 agtcacggct ggaaatcgta atgcgcacct attttgaaaa accacgaact gttgtcggct
     4081 ggaaaggact aatctccgat ccagatttaa acggcagcta tcgggtaaat cacggtctgg
     4141 agctggcgcg caaattactt ttacaggtaa atgagctggg cgtcccaacc gcgactgagt
     4201 tcctcgatat ggtgaccggt cagtttattg ctgatttaat cagttggggc gcgattggcg
     4261 cacgtactac cgaaagtcag atccaccgtg aaatggcttc ggcactctcc tgtccggtag
     4321 gttttaaaaa tggtaccgat ggcaatacgc ggattgctgt ggatgctatc cgcgcagccc
     4381 gcgccagcca tatgttcctc tcgccagaca aaaatggtca gatgaccatc tatcagacca
     4441 gcggcaaccc gtatggccac attattatgc gtggcggcaa aaaaccgaat tatcatgccg
     4501 atgatatcgc cgcagcctgc gatacgctgc acgagtttga tttacctgaa catctggtgg
     4561 tggatttcag ccacggtaac tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg
     4621 atatttgtca gcaaatccgc aatggctcta cggcgattgc tggaattatg gcggaaagtt
     4681 tcctgctcga aggaacgcaa aaaatcgtcg gcagtcagcc gctcacttac ggtcaatcca
     4741 ttaccgaccc gtgtctgggc tgggaagata ccgaacgcct ggtcgaaaaa ctcgcctctg
     4801 cggtagatac ccgcttctga atgcgtgccc attcctgacg gaatgggcat ttctgcgcaa
     4861 cttgttgtct tctcaacaaa ttactgcttg ctctggcctg ccataatatt gataataaga
     4921 atcattgtta tatcaattat tattaatctt tatgcgttat acggatagca gaaaactcac
     4981 gcctgaaacg gacgccaatc acaaggccgc ttccccgcag cccatccggc gaattaccag
     5041 ccagacactg ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct
     5101 gctccgtaaa actcaggctg gcaagctgct gttaactaag tagcctttaa ctcgagcagc
     5161 tgacttccag ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctatcgc
     5221 tgaaaggatt tcgcaacgcc ccatgcaggc ggtgcaattc cgtcatatca cccttttctg
     5281 ccgcttcaat cgcccgttgc gccaaccagt tgcgcaatac cagagcgggg ttaacgcttt
     5341 gcatcaactg ctgacgctca ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac
     5401 gggcaaacca gccatcaaat gccgcacgat caataaactc atcacgtagc ggtgacgccg
     5461 tgctgtgctg ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc
     5521 gcgccatcag actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtgatga
     5581 agcccagttt ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt
     5641 ccagggcctc attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct
     5701 gtaaattcca caacgcgaca gcaggttgat tatcaaaact ataacgccct tgatgatccg
     5761 agtgattaca aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa
     5821 gcgtcagtcc cagcagcgac atgttgtcgg tattcatcac cccatgtgca aagccgaccg
     5881 tctgccattg ggcaattaac gacgcggtac gtgcgacaac atcgctaaac cagagacggt
     5941 atttgtcctc atcatcttca agatgtgacc agtaatgacg aatagcaaag tcagctaact
     6001 gacgaacctt ttccgactcg cggcggtagt aaaaatgttc gaaatgacca aagcgcagat
     6061 gacttggtgc cacacgcatc agcatcgcgc ctggctccgc cgtttcccga tacactggcg
     6121 aatcgctggt gacgatactt aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct
     6181 cactggcgag actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg
     6241 aataaggcgt caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagca
     6301 gttgttcgcc gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact
     6361 gatgaccact gtaaacctgc gccagtggtg acataccagg cagtagggct tcgccgcccc
     6421 agacacctgc gccattttta aacagcgacg atggaatact cagcgtgtta gccagttcgg
     6481 tattatgcca aatcagccgg gcattattta aaggcgtagg ggaaagtgct gtataggttt
     6541 ctggcaattc atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat
     6601 agtgtagacg gttactctcg tcttaaacac cagcaaacag aagggttatc gctgaaccaa
     6661 cgtcgttatc tgatttatgg gaacggcagg ccacaggcat ccctggagag catggaaatc
     6721 gaagggggta atctgtgcca atatttctgc cgtatcaatg cctcccgcaa tgatgcaatt
     6781 gcaacaaggg gaaatttgcg cctggatcgc acgaataaac ggttcgaagg agcggtgcgt
     6841 gatttgttgc tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac
     6901 cgctttcatt gtgctattgc ctgctcccaa attacctaac acaaggggat acacttgcga
     6961 taaggataaa agaccgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat
     7021 caacagttca ataaacggat attttaatag ttcgcctgcg taattatccc tctctaataa
     7081 taatgtagcc acttgtggcg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa
     7141 atgttggcat gattttagta attccaactg ctctgaaaat aattgccaat gttgctcctc
     7201 ggtaagttgc gctataaccc gactcgtcgg gatacgcacc gtgccatctt cactggagaa
     7261 atgggtaatt aactcaacgc cgacaaggac ttgctggtta tctcttatcg gcaggaaata
     7321 gcaatcagaa tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg
     7381 atgtttttca gtatccggct aaaagggatg aggccataag acataacaat tacagaagga
     7441 gtaactttca tttgttccat gttaaccact ttttcagggg tccttttttt agattatcct
     7501 gattataaac gaataatcca tttacggaat ttttgtctgc aaaatactac tgtattcagg
     7561 agtaaatgga cggcgatgta tcatcgccgt ccatttttcg aca
//
LOCUS       DAJVYM010000172         7520 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVYM010000172 DAJVYM010000000
VERSION     DAJVYM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.174"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(937..1149)
                     /locus_tag="OQS60_003501"
     CDS             complement(937..1149)
                     /locus_tag="OQS60_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCS2389175.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1225..1842)
                     /locus_tag="OQS60_003502"
     CDS             complement(1225..1842)
                     /locus_tag="OQS60_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS2389176.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2109..3608
                     /gene="ansP"
                     /locus_tag="OQS60_003503"
     CDS             2109..3608
                     /gene="ansP"
                     /locus_tag="OQS60_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS2389177.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3723..4784)
                     /locus_tag="OQS60_003504"
     CDS             complement(3723..4784)
                     /locus_tag="OQS60_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS2389178.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5026..7128
                     /locus_tag="OQS60_003505"
     CDS             5026..7128
                     /locus_tag="OQS60_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS2389179.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7164..>7520)
                     /gene="mcbR"
                     /locus_tag="OQS60_003506"
     CDS             complement(7164..>7520)
                     /gene="mcbR"
                     /locus_tag="OQS60_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS2389180.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1925 a         1740 c         1812 g         2043 t
ORIGIN      
        1 gagagatcga gggaaaagag cgaagagagt gactggttca aatgaaagga aaccaccgca
       61 aaagcatccg gtggcaggcc gtccacttcc agtgtgaaac gtaatccggt tgacatagca
      121 acctccgtga aaatgaataa taatccgcac atattgcatg tacaacatgt cgggcggatg
      181 agataagcga gtcgacatct ggcgttaccg gggaatatca gaaaaatgtc atggttatgt
      241 gatgatttag tctgggattt tacttcagag aaacgggtgt ctgtttagtc aacaaaataa
      301 tcacaaacgc aacggatggg ttctcgacgt tattcacgac aggctcgtac attacagcga
      361 gtaataaaaa acctgatatg caaagtcaaa ccaatattca taatgtcttt ttattcacaa
      421 caataaataa ttcaataaca atcattgaaa tgaatgatgg tttgtataag gaatatacgc
      481 taccgcccac cctgataaca tgatctgcac attgccatct taacgtcgtt aaacactggc
      541 ttaatagtgg atatttcaaa ttaggaattt attaaattca cctgatgaat ataataagca
      601 aatagcatag aaaataatgg caactccccc cgagaggacc atttccaaag cactgcacca
      661 ccacctggtg cccggatgat cttgcttcag tggtaagagt acctgattca ttgttcccaa
      721 aatgatgttt tccccggccc cgccaataat gtgcagctct cacgtgataa tctattgatt
      781 aggttatatt aagaatcaat atttaaaata catccatttt cttgaataaa tcaacatatt
      841 tatttatcaa ttgctgtttt agctctgact catcaagctc tccatattta tcctatagat
      901 aattgcgaat aaatacagag aacgccattt atccttttag catacctgcc ggatattttt
      961 atttgcatta atattaattg catctccaac tccgagcata tccatcatta aaaacttttt
     1021 gagattatag aatacctgtg ataacaagtg aaacatctgc acaatgtgaa tagttagttt
     1081 cttttcagag gaaatgaaat agacaagctg actgaagggg agagtgatat aaatcaaaaa
     1141 acataacatg aatttaatta attacgccca tcattgtgga taaagttact gaataataaa
     1201 taacataatc ctctggtatc ccactcaaat aatttcattc ctttttaata cttcttgtaa
     1261 tttcggtaac tgacatacag catcagcaat cgcactgata ttcgtggcat tatcctgaaa
     1321 ccattcatgc ccagaccccc atgtgcgcat agtgcaaaga taacagtcca ccagcgttag
     1381 ttgctcacca aacgcataag gttcagcggt caattgtgaa ttcagccaga tataaagcga
     1441 tttacgatat tcaataacat tcttctttaa ctgttcaggt gcgtcaggag cccaacgctc
     1501 agggtaatcg gcgaaagtga atgttggata gacattagca accagccaga ccaatagccg
     1561 ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca aggtccgggc gacgatcgag
     1621 aaccatcaat gcaatcgccg ctgtctccgt cataatctcg tcattttcca gcgccagtgt
     1681 cggaatctga cacaacgggt tcagggtttt caataactcg cgtgaagttc cctcgtggtc
     1741 aaaaccactg acatcaacga actgataagg aatatcagcc agtgttagca tcagctcact
     1801 gattgtagag ccccatccgg gtacgccata caccttaatc atatgcccct cacaatcttt
     1861 taaagattaa gtgtaggaca gtacacaggt taaccagcaa aagtatttgt cattttttcg
     1921 tcagttgtat gaatcccctt caggattgta aggggatgtt ttgcatcgta tatcgataac
     1981 gggtaggatg ctgcgccgat agttttatac ccataaagaa taatggtgat aactatcatc
     2041 gccaggatga ataaacattg ttcatggcaa cttatatgac tttttcatta aagcaatcag
     2101 ggagagcaat gagtaaacac gacaccgaca cttcagatca acacgccgcg aaacgccgct
     2161 ggcttaatgc ccacgaagag gggtatcaca aagcgatggg caatcgccag gtacagatga
     2221 tcgccattgg cggcgcgatt ggcaccggct tgtttttagg tgcaggagcc cgactgcaaa
     2281 tggcgggtcc agcactggca ctggtttatt taatttgtgg cttgttttcg ttttttattc
     2341 tgcgtgcatt gggtgagctg gtgctacacc gcccttccag tggcagtttt gtctcttatg
     2401 cccgtgagtt tttgggtgag aaagccgctt atgttgctgg ctggatgtac ttcatcaact
     2461 gggcgatgac ggggattgtt gatattaccg ccgtcgctct gtatatgcat tactggggtg
     2521 cgtttggcgg cgtgccgcag tgggtctttg cgctcgctgc acttaccatc gttggcacca
     2581 tgaatatgat cggtgtgaaa tggtttgcgg agatggagtt ctggtttgcg cttattaaag
     2641 tgctcgccat tgtgaccttt ttggtcgtgg gtacagtgtt cctcggtagt ggtcagccgc
     2701 tggatggcaa caccactggc tttcatttaa tcaccgataa tggcggcttc ttcccccacg
     2761 gtttgctgcc tgcgctggtg ttgattcagg gcgtagtgtt tgcttttgcc tccattgaaa
     2821 tggtgggtac agctgccgga gaatgtaaag atccgcagac catggtgcct aaagccatta
     2881 acagtgtgat ttggcgtatt ggcctgtttt acgtcggctc cgtggtgttg ctggttatgt
     2941 tattgccgtg gagcgcgtat caggcggggc aaagtccgtt cgtgacgttt ttctctaaac
     3001 tgggtgtgcc atatatcggc agcattatga acattgtggt gctgaccgct gccctctcca
     3061 gcctgaactc aggtctgtac tgcaccgggc gtattctgcg ctcgatggcg atgggcggtt
     3121 ccgcaccgag ttttatggcg aaaatgagtc gtcagcatgt gccgtatgcc gggattctgg
     3181 cgacactggt tgtgtatgtc gtcggcgtat tcctcaacta tctggtgccg tcgcgcgtat
     3241 ttgagattgt gttgaacttc gcgtcgctgg gaatcatcgc ttcatgggcg tttatcatcg
     3301 tgtgccagat gcgcctgcgt aaagcgatta aagaaggcaa agcagcggat gtcagtttta
     3361 aactgcctgg cgcgcccttc acttcctggc tgacattact gtttttactg agtgtccttg
     3421 tgctgatggc gttcgattac ccgaacggga cttatactat cgcggcgctg ccgattatcg
     3481 gtattctgct ggttataggc tggtttggtg tgcgcaaacg cgttgctgaa attcacagca
     3541 ctgcgccagt cgtcgaggaa gatgaagaaa aacaggaaat tgtgtttaag cctgaaacgg
     3601 cgagctaatt gcaaaattgc ccggctacgt gccgggcaaa tttagattac tgacgaaggt
     3661 gtgccgcatg cggcgtgata aaagtcaggc aagtctctcc gttttcaccc tcgccatatc
     3721 tattacagcg caatacgaat cacatcgtct ggctgggtag cttcctgctg tttagtggat
     3781 ttttgtttca cactgacata cagcgttttg ccatcggcag acagcgccag gctgtttgga
     3841 tgagtcggcg tatcgaacgt tttcactact ttatagcttt tcgcgtcaat cacactgact
     3901 ttacctgcct gacgatgcgt tacgtaggct tcattacgcg ctgggttaaa cagcacagcc
     3961 agtgattccg gtgctgcaac cttcgccaga atattgccat tacgggtatc gaccactaac
     4021 acttctgcgg ctttagaatc ggtgataaat gcacgctgcc tagcggtatc aaggctaatg
     4081 ttgataaaga agtgctcttt gccgtcatcc agcagctttt tacggctgag gattttattg
     4141 tcggcggtgt cgatggtaat caattcgccg tcagcgttag tggtgtaaag acgtttgcct
     4201 ttgctatcaa gcgccagacc ggtactcatt ttaccggtgt tctggatggc ggttttcagt
     4261 ttgatattct cgccatcaac gacccaaatc acgctctctt taccaatacc actgatgtaa
     4321 acggtgttcg tggcatcgtc agctaccagc tcacgcggtt gcagcgggcg cacctcttcc
     4381 gtacgcttac gatcatccag caccagacgg cctttcacct cgcccgtttt ggcatctatc
     4441 gccgtgaccg cgctgtttac agtgttacca aaccacagtg tctgagtcgt gttattgatg
     4501 gtgacaccaa acggcttgag atcgttatgg atcgcctgcg tcacttccag agtgaccgga
     4561 tcaagacgat aaaccacgcc gcctttatcc agtttgcggc tttgcgaagt ggcgagccac
     4621 agcgcgtttt cttgctggct ataagccatt tcgtaggcac ctttacctac cgctttacgc
     4681 agcatttctt ctgcggcctg cgtactgaat gatgaagcaa caagcaatga acctaacagt
     4741 aatgaaccac gcaggcgcga tgaaaacaga tgacgtaaat gcatgacgac tccctttgat
     4801 gaaccgatat ttttgctgct tcacaatgct tcgctaagcg taatcatcgt tacgctcttg
     4861 ttataaatgg gaatggtaat cattattttc tcaaatgtgg agcatttctt gccgttacga
     4921 ctacaatttt ctcaattact taacgtatcg tgccgtttcg ttttccatga atttacaatt
     4981 ttattaccat aacggcgttt tcccattctt aacctggctt ttttcatgaa gattttttcc
     5041 gtccgacaga ccgttttgcc cgcactactt gccctttccc ccgttgtttt tgccgctgat
     5101 gaacagacca tgattgtcag tgccgcaccg caggtggttt cagaactgga taccccagca
     5161 gcagtaagcg tggtggatgg cgaggagatg cgcctggcaa caccgcgcat taacttgtcc
     5221 gaatcactga ccgacgtgcc tggtttgcag gtacaaaacc ggcagaacta tgcgcaagat
     5281 ttacagctgt cgattcgcgg atttggctcc cgctccactt acggtattcg cggtattcgc
     5341 ctgtatgtgg acggtattcc cgccaccatg cccgacgggc aagggcaaac atccaacatc
     5401 gatttaagca gtgtgcaaaa tgtggaagtg ctgcgtggcc ccttctctgc cctgtatggc
     5461 aacgcgtctg gtggggtaat gaatgtcacc acccagaccg gacaacagcc accaaccatt
     5521 gaagccagta gttactacgg cagttttggc agctggcgct atgggctgaa agcaacgggc
     5581 gcaacgggag acggcacaca gcctggcgat gtcgattaca ccgtctcaac cacgcgtttt
     5641 acgacccacg gctatcgtga ccatagtggc gcacagaaaa atttagccaa tgccaaactg
     5701 ggcgtacgca ttgatgaagc cagcaaatta agtctgattt tcaatagtgt ggatatcaaa
     5761 gcagatgacc cgggtgggct aaccaaagca gaatggaagg ctaatccaca acaagcgcct
     5821 cgtgcagaac agtacgacac gcgaaaaacc atcaagcaaa ctcaggctgg gttgcgctat
     5881 gagcgtagcc tgagcgcgca agatgatatg agtgtgatga tgtatgccgg agagcgagaa
     5941 acgacccagt accagtcaat acccatggca ccacaactta acccgtcaca tgcgggcggc
     6001 gtgattaccc tgcaacgcca ttaccagggg atagacagcc gctggacaca ccgtggtgaa
     6061 ctgggcgttc cggtcacgtt cactaccggc ctgaactacg aaaacatgag tgaaaaccgc
     6121 aagggctaca ataacttccg cctgaatagc ggcatgccgg agtacgggca aaaaggtgag
     6181 ttgcgtcgcg acgaacgcaa tctgatgtgg aacatcgatc cctatttaca gacgcagtgg
     6241 cagctgagcg aaaaactgtc gctggatgct ggcgtgcgct acagctccgt gtggtttgat
     6301 tccaacgacc attacgttac tccgggtaac ggcgatgaca gcggtgatgc cagttatcat
     6361 aaatggctac ctgccggttc gttaaaatat gcaatgaccg atgcctggaa tatctatctg
     6421 gcagccgggc gaggttttga aacgccgacg attaatgagc tgtcttatcg tgctgatggg
     6481 caaagcggta tgaactttgg tttaaaacca tccaccaacg atacaattga gatcggcagt
     6541 aaaacgcgta ttggtgatgg gctgcttagt ctcgcattgt ttcagaccga cactgatgat
     6601 gaaattgttg tcgatagcag tagcggtggg cgtacgactt acaaaaatgc cggaaagacc
     6661 cgtcgtcaag gcgctgaact ggcatgggat caacgtttcg caggagattt tcgcgtaaac
     6721 gcgtcctgga cctggcttga tgcgacctat cgcagcaatg tttgcaatga acaggattgt
     6781 aacggtaatc ggatgccagg gatcgcccgt aatatgggct ttgcgtcgat aggttatgta
     6841 ccggaagatg gttggtatgc aggcacggaa gcgcgttata tgggcgatat tatggcagat
     6901 gatgaaaata cggcaaaagc gccgtcttat actctcgtcg gcttattcac cgggtataaa
     6961 tacaattacc acaatttaac tgtggattta tttggtcgtg tcgataattt attcgataaa
     7021 gaatacgttg gttctgtcat tgtcaatgag tcaaacgggc gatattacga accttcgccc
     7081 ggacgaaatt atggtgtcgg catgaatatt gcgtggagat ttgagtaatt accttgatgc
     7141 ccggtatttg ccgggcattt actttaacga ttgtattgct ggtataaaat agcaatattt
     7201 tgttgcataa tttcagcaag acaatgtctg catccctctt tgtcttttgc ttttaacgca
     7261 gcgagtaaca gatgatagtt ttctatatgc tcccgtaatt ccgctggatt aatcgcttca
     7321 tagagataat gtaaaccagg ccccatcctg acccacagtt gctcaatcat ctcacacagg
     7381 atgggcatat ttgagcgatg ataaatcgct aagcgaaata gcctgtttac attaatgatt
     7441 tgttccatgt cgcccttttc ttgcgcctgc tgtaatttct cgagcaattc ctggagttcc
     7501 gtcaggtctt gcggggtgag
//
LOCUS       DAJVYM010000173         7121 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVYM010000173 DAJVYM010000000
VERSION     DAJVYM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.175"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            4..435
                     /locus_tag="OQS60_003507"
     CDS             4..435
                     /locus_tag="OQS60_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS2389181.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OQS60_003508"
     CDS             518..1420
                     /locus_tag="OQS60_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS2389182.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OQS60_003509"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OQS60_003509"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS2389183.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OQS60_003510"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OQS60_003510"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS2389184.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OQS60_003511"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OQS60_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS2389185.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OQS60_003512"
     CDS             complement(5330..6037)
                     /locus_tag="OQS60_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS2389186.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OQS60_003513"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OQS60_003513"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS2389187.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OQS60_003514"
     CDS             complement(6895..7098)
                     /locus_tag="OQS60_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2389188.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1991 c         1853 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAJVYM010000174         6835 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVYM010000174 DAJVYM010000000
VERSION     DAJVYM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.176"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(119..883)
                     /gene="nfeF"
                     /locus_tag="OQS60_003515"
     CDS             complement(119..883)
                     /gene="nfeF"
                     /locus_tag="OQS60_003515"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS2389189.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1171..1794
                     /gene="nfeR"
                     /locus_tag="OQS60_003516"
                     /gene_synonym="yqjI"
     CDS             1171..1794
                     /gene="nfeR"
                     /locus_tag="OQS60_003516"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS2389190.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(1901..3421)
                     /gene="aer"
                     /locus_tag="OQS60_003517"
     CDS             complement(1901..3421)
                     /gene="aer"
                     /locus_tag="OQS60_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS2389191.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            3839..5218
                     /gene="ygjG"
                     /locus_tag="OQS60_003518"
     CDS             3839..5218
                     /gene="ygjG"
                     /locus_tag="OQS60_003518"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS2389192.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5260..5592)
                     /locus_tag="OQS60_003519"
     CDS             complement(5260..5592)
                     /locus_tag="OQS60_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS2389193.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            5811..6794
                     /gene="ebgR"
                     /locus_tag="OQS60_003520"
     CDS             5811..6794
                     /gene="ebgR"
                     /locus_tag="OQS60_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS2389194.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1664 a         1843 c         1723 g         1605 t
ORIGIN      
        1 tcagtggtta gagcaggcga ctcataatcg cttggtcgct ggttcaagtc cagcaggggc
       61 caccagatat agcaaaggct gacgagaaat cgtcagcctt tttcttttta tatatcagtt
      121 actttgcgtg ccagtaagcc gctgcacgta cccgctgtgg gtcatactgt tccgcttcaa
      181 agcggcggct taaattctta acgactttac cttcgccggt tatccagatg aagtaatcat
      241 cggcagggat ttgcatctgc gccagacgcg catctaccgc ctgctcatca tgtgccagcc
      301 attcgatatt aaaaccatca aggtgcgcga gataatcctg acaggcgtta tcccgcacgc
      361 taaccagcgc actaacttgc ggtttaacgg caagtttgct caacgtttcc aagcggcggc
      421 gcagtgcggg cattccggat tcatcgcaga catacagctg atacgcgtaa tcttccggca
      481 ccaccagcga accgcgcgga cctgcaaccg taagtttatc gcccggttgc gcctgcatcg
      541 cccagccgct ggcgacccca ccgtcgtgaa taaagaaatc gatcgccagt tcatggcgta
      601 gttcgtcata cagcggcgta tagtcacgcg acggtgggcg tggtccttcc ggccagacga
      661 tgccctcttc cgttaccgtt ggcggcacaa agtgagcgtc aggttgagga aagaagagtt
      721 tgctgtgatc gtcaaagccg cgcgatgtaa aaccgtccag cgcctcgccg ccgaggacaa
      781 tgcgctgaaa accggcgctg atgcgctcaa cgcgtaacac agtcagttca cggaagcgca
      841 gatcattgcg aacgcgctgc gggtagcggg gggagttatt cattgttatc gccttcgtga
      901 tggtaatcag atatatctaa ataattttcg taaatgataa tgattgttaa tcatgataaa
      961 tgcaagcgat ttgtagaact gatatgtcta tagtctgata agacgaaccg cctcttctca
     1021 ggcatcatta ctcaacgccg gatgcggcgt gaacgcctta tccggcctac gtgtgagatg
     1081 agtccacatt taaaaatcat ttttattctt gcaagagtgc tcatatcaga tataaattag
     1141 atatatctaa tcaagcaaaa ggaggctgat atgagccatc atcacgaagg gtgttgtaaa
     1201 catgaaggcc agccacgcca tgagggctgc tgcaaaggtg agaagtcaga acacgagcac
     1261 tgcggacacg gtcaccagca tgaacacggt caatgctgcg gtggtcgcca cagtcgcggc
     1321 ggcggtcgtc ggcaacgttt ctttggtcac ggtgaattgc gtctggtgat tctggatatt
     1381 ctctcgcgcg atgacagcca cggttacgaa ttgattaaag cgattgagaa tctaacccag
     1441 gggaattaca ccccaagccc gggcgtcatc tacccaacgc tggattttct gcaggagcag
     1501 tcgctgatta ccatccgcga agaggaaggt ggtaagaagc agattgcgct gaccgaacaa
     1561 ggcgcgcagt ggctggaaga aaaccgcgaa caggtggaga tgattgaaga acgcatcaaa
     1621 gcgcgttgcg ttggtgctgc gctgcgccag aacccgcaaa tgaagcgggc gctggataat
     1681 tttaaagcgg tgctggattt acgcgtcaac cagagcgata tcactgatgc acaaataaaa
     1741 aagatcattg cggtgatcga ccacgccgct tttgatatta cccaactgga ttaatcaccg
     1801 attgccggat gcggcgtacc ctacacaccc cgcaggcctg ataagatgcg ccagcatcgc
     1861 atcaggcatt gtgctccaac cgccggatcc ggcataccga ttaatgcagc accgtcaccg
     1921 cgtcttccag tcggctggcg cggtgtttca ccatcgccga cacctgcgca ctctcttcca
     1981 ccagctcagc atttttctgg gtgatcaggt taagctcatc cactgcacgg gtcaggctgg
     2041 aaagcccatc ggcctgttcc agcgttgaat ggctaatctg ggcgatcaac tgggtgacgt
     2101 ttttcacctg tgccacaata tcttccatcg tccgtccggc ggcgtgtacc tgctgcgaac
     2161 cggattgcac cttatcagca ctggcatcaa tcagcttgcg aatatcgttg gcagcattag
     2221 cgctgcggct ggctaaatga cgcacttccc ctgccaccac tgcaaaacct ttgccctgtt
     2281 cgccggcacg cgccgcttcc accgccgcat tcagggccag aatattggtc tgaaacgcaa
     2341 tatcgttaat cagcgaagta atggtgccaa tgcgctgggt actgtcggcg atatcgtcca
     2401 ttgtcttgat caccgtggtc atcgcctccc caccctgcac tgccgcatta ctggcggtga
     2461 tagaaagttt atcggcagcc gacgccgtgg cagagttctg tttcaccgac gccgccattt
     2521 ggttcatggt cgccaccgtt tgctgaacgt tatcaactgt ctgctgggta tgttcgttca
     2581 gttcatcggt gcctttcgcc agcgtctcac tgccatttct gacactggac acctggcttg
     2641 agacatcgtt aattagccaa cggcacatca ggccaagctg ccctaccgcg cgtaatgtca
     2701 gccccagctc atcgctgcga ttcaggtgct caacactatt acgctctccg gtcgccacct
     2761 tcagtgcctg acgggcgaca ttttctatcg ggcgcacaat ctgccattca aaacaagcgc
     2821 ttgccaacaa taccactaac acacagagga aatacgtcac caccggggca gcaacaaacc
     2881 aaagcatggc cgccagcaag ataaacatca gggtcatcac tccacgcgcc cgccagcgaa
     2941 gcggtaatga aggcagttta cccagccagc ctttacgcac caccaggcct ttatgaatac
     3001 gcttactggt acgtccggcg ttcagcgctt tgtacagcgg ctccaccgcc gcaatctctt
     3061 catccgtcgc ccgggtacga atcgacatat agccactgat ttttccctcg cgcaccatcg
     3121 gtaccgcatt ggcccgcacc caataatggt cgccattttt gcggcgattt ttcacgatgc
     3181 cgctccaggg ctcccctttt ttcagggtga accacatatc cgcaaacgcc gcttttggca
     3241 tatccgggtg acgcaccatg ttgtgcggct gcccttgtaa ctcttgcaag gtaaagccgc
     3301 tcacctgcac aaaagtgtca ttagcatgag tgatatagct ttgcagatcg gtagtggaca
     3361 tcagagtggt atcgtccgcc agcggggtat tttgctgggt gacatacgga tgagaagaca
     3421 tgttcgcgtc ctgtgcaggt tatatggttg ttaacttctt gtcagagttt atgtcggccc
     3481 cgctgcggtt atctttaacc gattaatttg atttagatcg caatttgcga ttaaagcaca
     3541 aatctaattc tttgatttaa aatactttca ctctgttact atacgaaaac gttaattatc
     3601 ttgcccaaaa atcaggcaat tattgccctg aaaacgtgca tttgcgcagc aatcatcaaa
     3661 tccatacccg acaaaaaccg tgcaaaataa caacaaatgt taacagatag cattaaatat
     3721 tgcacaaatg ataaccgaat ttgtgtttat cccgattttc gcgatcgcag ccggagtggc
     3781 gcaatccctg caatacttaa atcggtatca tgtgatacgc gagcctccgg agcatatttt
     3841 gaacaggtta ccttcgagcg catcggcttt agcgtgcagc gcccacgccc tgaatctcat
     3901 tgagaagcga acgctggatc atgaggagat gaaagcactt aaccgagagg tgattgaata
     3961 cttcaaagag catgtcaatc cggggttttt agagtatcgc aaatctgtta ccgccggcgg
     4021 ggattacgga gccgtagagt ggcaagcggg aagtttaaat acgcttgtcg acacccaggg
     4081 acaggagttt atcgactgcc tgggaggttt tggaattttc aacgtggggc accgtaatcc
     4141 agttgtggtt tccgccgtac agaatcaact tgcgaaacaa ccgctgcaca gccaggagct
     4201 gctcgatccg ttacgggcga tgttggcgaa aacccttgct gcgctaacgc ccggtaaact
     4261 gaaatacagc ttcttctgta atagcggcac cgagtccgtt gaagcagcgc tgaagctggc
     4321 gaaagcttac cagtcaccgc gcggcaagtt tacttttatt gccaccagcg gcgcgttcca
     4381 cggtaaatca cttggcgcgc tgtcggccac ggcgaaatcg accttccgca aaccgtttat
     4441 gcagttactg ccgggcttcc gtcatgtgcc gtttggcaat atcgaagcca tgcgcacggc
     4501 tcttaacgag tgcaaaaaaa ccggtgatga tgtggctgcg gtgatcctcg aaccgattca
     4561 gggtgaaggt ggcgtaattc tgccgccgcc gggctatctc accgccgtac gtaagctatg
     4621 cgatgagttc ggcgcactga tgatcctcga tgaagtacaa acgggcatgg ggcgcacggg
     4681 caagatgttc gcctgcgagc atgagaacgt acagccggat atcctctgcc ttgccaaagc
     4741 gctcggcggc ggcgtgatgc cgattggcgc gaccatcgcc actgaagagg tgttttcagt
     4801 tctgttcgac aacccattcc tgcataccac cacctttggc ggcaacccgc tggcctgtgc
     4861 ggcggcgctg gcgaccatca atgtgttgct ggagcagaac ttaccggctc aggctgatca
     4921 aaaaggcgat atgttgctgg acggtttccg tcaactggcg cgggaatatc ccgatctggt
     4981 acaggaagcg cgtggtaaag ggatgttgat ggcgattgag tttgttgata acgaaatcgg
     5041 ctataacttt gccagcgaga tgttccgcca gcgcgtactg gtggccggaa cgctcaataa
     5101 cgccaaaacg atccgcattg aaccgccact gacactgacc attgaacagt gtgaactggt
     5161 gatcaaagcg gcgcgtaagg cgctggcggc catgcgagta agtgtcgaag aagcgtaata
     5221 tcaaatcgga tggcgatacg acgtcgccat ccgatctttt tatacgacgc gtacgcccgc
     5281 aggcatcatc cgctccggcg ttaacagcac gctttcactg ccgtcatcgg tttccgcaca
     5341 caacagcatg cattccgacg tttcaccacg cattttcgct ttttgcagat tgcataagac
     5401 caccaccgtt tttcccatca gctcctcttc gctgtagtac ggcaccaggc tggtcacggt
     5461 ttgcagtgtt ttttccccca catcaacctg tacgatgtac agcttgtcgg cgttttcatg
     5521 gcgtttcact tccacaatct ttccgacgcg catttccaga cgtgcaaaat cagcgtaagc
     5581 cacggtttcc attgcttcct cccttcaagt aaaattttac taaactatag caaaattttt
     5641 tctcaatcct gtaggctaaa aatggagaat gcaggcgtga tcacattcgt aagccgctgt
     5701 gttaccgtta cagcgtcaaa gaaacgcgct ttatttactg aaaacaggtg acccgataag
     5761 cacttcctct acaatggggg cgcacatcag ggaaagtaaa aaaggtaaac atggcaacat
     5821 taaaagacat cgcaatcgaa gctggcgtat ccctggcgac agtatccagg gtcttaaatg
     5881 acgatccgac attgaatgtg aaagaagaga cgaaacatcg cattctcgag atcgccgaaa
     5941 agctggagta caagaccagt agcgcccgta aactacagac aggcgcagtc aaccaacacc
     6001 atattctggc tatctacagc taccagcagg agctggagat caacgatcct tactatctgg
     6061 cgatccgcca cggcattgaa actcagtgcg aaaagctggc gatcgaactg accaactgtt
     6121 atgaacacaa cggattacca gacattaaaa acgtcaccgg tattttaatt gtcggcaaac
     6181 ccacgcccgc cctgcgcgcc gctgccagcg cgttgaccga caatatctgt tttatcgact
     6241 ttcacgaacc cggcagcggt tacgatgcgg tggatatcga tctggcacgc atcagtaaag
     6301 aaatcatcga cttctatatc aaccagggcg ttaatcgtat tggttttatt ggcggtgaag
     6361 atgagcctgg caaggcggat attcgtgagg tcgcctttgc ggaatatggc cgactgaaac
     6421 aagtggtacg cgaagaggat atctggcgcg gcggtttttc cagttcgtcg ggttatgaac
     6481 tggcaaaaca aatgctggcg cgggaagact atccgaaggc actgtttgtt gcttccgatt
     6541 ccattgctat cggcgtactg cgggcaattc atgaacgtgg cctgaacatc ccacaggata
     6601 tttcgctttt cagcgttaac gatatcccca ccgcgcgatt tacctttccg ccgctctcca
     6661 ccgtgcgcat ccattccgag atgatgggaa gtcagggcgt taacctggtg tatgaaaaag
     6721 cccgcgatgg tcgcgcgctg ccgctgctag tcttcgttcc cagcaaatta aaactgcgcg
     6781 gcacgacccg ttaaatcccc ttacacactg tccggcaatc atttttgccg gacag
//
LOCUS       DAJVYM010000175         6788 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVYM010000175 DAJVYM010000000
VERSION     DAJVYM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.177"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..302
                     /locus_tag="OQS60_003521"
     CDS             <1..302
                     /locus_tag="OQS60_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS2389195.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="OQS60_003522"
     CDS             complement(391..903)
                     /locus_tag="OQS60_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS2389196.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="OQS60_003523"
     CDS             1098..1862
                     /locus_tag="OQS60_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389197.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="OQS60_003524"
     CDS             1813..3216
                     /locus_tag="OQS60_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS2389198.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="OQS60_003525"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="OQS60_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="OQS60_003526"
     CDS             4573..5316
                     /locus_tag="OQS60_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS2389199.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="OQS60_003527"
     CDS             5320..6540
                     /locus_tag="OQS60_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS2389200.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..>6788
                     /locus_tag="OQS60_003528"
     CDS             6544..>6788
                     /locus_tag="OQS60_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389201.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVR"
BASE COUNT         1800 a         1622 c         1857 g         1509 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgcca
//
LOCUS       DAJVYM010000176         6774 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVYM010000176 DAJVYM010000000
VERSION     DAJVYM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.178"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(281..424)
                     /locus_tag="OQS60_003529"
     CDS             complement(281..424)
                     /locus_tag="OQS60_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCS2389202.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(522..773)
                     /locus_tag="OQS60_003530"
     CDS             complement(522..773)
                     /locus_tag="OQS60_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HCS2389203.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(841..2511)
                     /locus_tag="OQS60_003531"
     CDS             complement(841..2511)
                     /locus_tag="OQS60_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HCS2389204.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2744..4963)
                     /locus_tag="OQS60_003532"
     CDS             complement(2744..4963)
                     /locus_tag="OQS60_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS2389205.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGIPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSNDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGVFCPVLVLPSPDYSHLMVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            5142..5417
                     /locus_tag="OQS60_003533"
     CDS             5142..5417
                     /locus_tag="OQS60_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCS2389206.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
     gene            complement(5633..6526)
                     /locus_tag="OQS60_003534"
     CDS             complement(5633..6526)
                     /locus_tag="OQS60_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS2389207.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
BASE COUNT         1594 a         1879 c         1562 g         1739 t
ORIGIN      
        1 aaaaaaaaca aaaagacaag gaaacaccag ggtaaacata aaaaatgcgt cagaatcgtt
       61 tttagcgcgt tgcataacta tgcacgaaag cgatgcacga tcggtttcgt gcgttctgaa
      121 caccgaacgg ctcaaaacga caagttttct ccccctggat ggaaagttct gcacggggat
      181 tttagatccc atctcccaca tggaattttt gtgacacttc aaaaaaaagc gcgtcagaat
      241 cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg
      301 tcagttcagc tgaggatgac ggggatacag tcccgccctg aacatcgcgg atatagtttg
      361 cctgacatgc agcaagaata atggctgcaa gaagcaataa aataatccat gttatttttt
      421 tcatactgtt ccttgtatat atggactcag gcattcatcc ataaaaacct gaaatccaaa
      481 ggttgaaatc atccatgaaa cataaaattc tataacacta tttacatctt taacgtgata
      541 atgaaagcag tgtatccatt ctcaatatca tctttaatat tttcaatagc caatctatta
      601 ttttcaggga tttttgattt gtattcctcc ggcatatcat atgtccatga tgctgaacta
      661 tctatcctga atccaggatt cagccaatgc ccaccatcaa tgcccaaaat ccataagcct
      721 tttttcgctg cttcattaca aacttctatt gcagcttttg gtgataactt catagaagca
      781 tttcctttta attcaaaaaa ggcatcacca ctatcatata acaccgtatg ttcaaataat
      841 ttattggtca tttttatttc cccattgaat tctcgaatcg tccacccaac ccggatctgt
      901 cttatcacta atctgagtaa cctcaccagt acgatcattg acaacaacat atttaccagg
      961 agaaccatac actgttgctg gatcatttcg acctaaatag tctggaggtg ttttcttcgg
     1021 actacgctta tcaaaagatg taccagttgc accgttagat acggtatttt tgatatcatc
     1081 ctctgtccat cctctttctg gcatttgtcc cctaattttc tgatctattt ttagacctgg
     1141 tatctttcct ttattttttg ctaactcatc atttagtttg ccttcagcat ctcttttatt
     1201 atcctctttc ttcttcctgc tttccatcgc agtacttaat gcagcatcag catctgcctt
     1261 ctcttttgca gcagcatcaa atgcagcctg cttattattt acatccgtct gcgcccgctg
     1321 agctttaagc ccggccattt gccacattct gtgaccgcca gccattgggt catgtgcaaa
     1381 tcgttcaaat tgttttattt cagctattgc atcagcaaga gttttattcg ctgcatcaag
     1441 ttcgctttta cgcgaattat aaacctgaac agctttagcc tgtcgctcct gatttctggc
     1501 aacatcttca tttgcctgat tcagctctgc acgcgcgcgt tcataatttc gctcagccgc
     1561 ttcaaccgga tgcgtagcat cccattcctg ctgacggcga ttttcttcat cctgacgttg
     1621 ttttacctgg tcagggctaa gaacatcact cactgaaaca tatacggcat tatgaccgct
     1681 gtccttcggg aatcgaataa ctgcatccct ggtattaccc ccctgagtaa atcctgccgg
     1741 gcgaacatcc ttatcagtat tatttgtaac acctgggctt aatgtctgta ccgctggcgt
     1801 actgttatta actgaaatat tcagaacagg tgcacctgga attgatgccg taaaaacacc
     1861 tggacgttcg gtaggttttg catcaaccac cggaacactc atcggaacac ctgaaacaac
     1921 cgaaatattc tgtcgttcgt cttttacatc atcaacaaca cgaacattta cgtttactgt
     1981 tgccttatcg agaggtaatg aactgacagg tgattcagta atatcatctg cgggtaatga
     2041 cgtcacaatc tttgacatca tattggggtc atctttcgct atttgtgatg gcaatacacc
     2101 atataaagcc accccccaaa gaccaaattt aaacggtcct ttcagggcag ccataatatc
     2161 agcaatagct gccgataatg ctcccgctga aatactgacc gccagaccgc cagctcctgg
     2221 agtggaaagt gccggaaaac caaatgccac tggcgcagct actgctgaca gattaccgcc
     2281 tgttcccgag ccaccaccgg aattaccatt ccccccgcca ttaccatgac cggaaccacc
     2341 cccccagtga atgccgctac cggaaccacc accccacggg ttattttccg aactccatcc
     2401 ggagccatca gaagcaccac cacctacacc aagcccggtc gggccaccat taatgttacc
     2461 acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg ccaccgctca taaaaactcc
     2521 ctcttttaag cgttaaaaca attaattaca tgattaatac tgtacataaa accactggtt
     2581 ttatgtacag atcaaaaagt acccgtcatc gttttttctg tcaagttcag gtggaaaatt
     2641 ttttagaaat caggagggat aacacttaga aaacgcagag cgggccacaa aaaattttgt
     2701 ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg atatcacata ctcatgcccc
     2761 ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc gtcccgcttc cactcatgcc
     2821 aggtctgatt aaaaccgagt tgacggaaga gataatccgg catctcttcc ggtttgtgga
     2881 cagcagccaa aagcctgtca tgatggcctt tgagccggaa ctgcatcagg tgcggttccg
     2941 gtgggtacgg agtatcctca tcatggttta tagcttctcc gtaacctgcc aggcataccc
     3001 cgttctccgt gatggtgctg accatcagat gactgtaatc cggcgacgga agcacaagaa
     3061 cggggcaaaa cactccttcc cggcgaacaa acccgacaag catttcctcc ggccctttgc
     3121 acaacaacag attcagcgtc ctgctgtttc cctctctcga aaatacaaca ggcatgagtt
     3181 gttccggtac agcatcagcg gtgatatcca cccggtgcgc tgacggtagc ggagaggcta
     3241 cgggcctgcg gtcgttgctg aaataaaaat tatccggcca gtgatgcggc agcgccggaa
     3301 aaccgtaaga gggccacccc tggcgcatct gttccagtac atgccggacc gtcgcaccgg
     3361 aatacattct cacacgctca ggccgcgcca tcgtctcatg agattctggt attccctcgc
     3421 gtatatccga gaccatcaca gccccgcctg gcgacaggta aacattcacc gggtcatgaa
     3481 cactctgggg gtaatgcgcc agctcatcac cccataccgt gaattctccg gctctgttac
     3541 tcaacatcat gtagcgggtt atcctgcccg ttcccggctc tctggcacgc cccagctccg
     3601 taataacccc gactaatgcc gtttcacggc tcagacggtg gttatcatga cgtttcagcc
     3661 gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc ggcttcctcc ctgcgccgct
     3721 ccgcttcccg tgctgacagt ttttctttca gctctgccat tcccggcgca atccacgtat
     3781 tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc ccgttctttc acggccctgt
     3841 aggtgcggcg aagagattca atctgtttcc cgagtttcca gaaacggagt tgtcttttgc
     3901 ggtattccgg ttcagcccat gtcatcaggg gaatctctga cagaagggac gcgacagggc
     3961 gtgctttttt gagtccggtc atctcctttt cgagaatggc ctgctctttc agcagcgcat
     4021 tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc caggaactcc ggggtcatct
     4081 cccacgcgtt cccgcattcc tgcggcagcg tggcaatgaa ttcccgcagg gtatccgctg
     4141 aggcattcgc agcgggaatg gtcaggttaa gggcttccgc tgcacgacgt gcagcatcgc
     4201 tgagttcccg cttacgctgc gcttcctgaa ccgtcctgag agcaagctgc gtgcgggaaa
     4261 cctgtatttc caggttaagg ctgttaatca gacggttctg ctcaccgcgt tctgtttcca
     4321 ttccccgttt ttccatcgca caggcagcct ttccgaggtg aaggcccggc gctttctcca
     4381 gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg gtagccggcg ttcgcaagcg
     4441 ctctgttcgc atggtcagcc catgatgcgc gccacgtctc ggcgagtgcc cggtcgttcc
     4501 agtccctgac ctttcctgcg aatcccgttt ccccgacagc tctcgtggtc agcatgatgt
     4561 gcgcatgggg attggtccgg tccatgtgat ggaacgccac atcggcaatc atgccccgac
     4621 tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg tgcggcctcg cgggaaatct
     4681 cacggggaat ggcaagttca atctcccttg ccagctggga gttccggcgc tgttcggctt
     4741 tctcgaccgc attccagaga acggaacggt caccacacca ggacggtgca ttcgcagggg
     4801 tgagaatgac cgcatcatca acgccgcgtt tacgggtgta atcgtggatt aatccggtac
     4861 ggtcatcggg tatgacagag ccggaacggt aggcagcaga agcaacagca ctgtagccgt
     4921 tttttcgcga aatgattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     4981 tttgccgttt tcttttagcg tcgcagaccg cacacaatcg aaacgaagtg gagattgtat
     5041 aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc agttttgcaa ctaccagtga
     5101 cgcgctaaaa tgcgattttt caccgcgcac ggagtcacga aatgagcaga acactcgaac
     5161 agaagattgc cgaagctgag gccagattac agcgcctgaa ggcgaagagc cggagcctgg
     5221 acacggcgca aaaagtcatt gtgggggctg ccatgctggc gagggtacgg aggccggaag
     5281 aagcgcaact gcgtgcgttc ctgctccagt ttctgcgaaa ggaagtcaca agacaggcgg
     5341 atgtgaaccg actacaaccg ctgattaacg agctggagat gctacccaga ccacccgcga
     5401 aaccgcagaa ccactaagcc ccggcgcgta agtgcccatg caccaccgga cgcctttgcg
     5461 cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagcg ctaccacctc
     5521 aatcatgcgg aaagccacgc cattacgggg cagaacggag aaaatcgcaa gaaagatcac
     5581 tgctcaaaaa gacgccaaaa gggcgctgtt atctgataag gcttatctgg tctcattttg
     5641 cgagatctgg atcacatttt aattttcggt agtacgtcgc tcgactgatc cctaatgcct
     5701 cccacggctt cagcgaacgt gctgatgtag gaactgctgc gcgcttagat ttagttccgc
     5761 ccttgcgacc tcgagcggcc tgtacagcgg aaaatccctc tggtgagaat ttcctgtgtg
     5821 tatatttcgc aatgctcttg ccaatagccc gacattcagc cggagaaagc ggaacgggaa
     5881 gcgatgcgtt gtacatttcg acacgctgga tcacggcatc aagccattgt gagaatacag
     5941 gccagccctg acgaatcgct ctgtaggccc atttacggac cttttcgaac agatggtagt
     6001 ttcgccccag cccgtaattt ttatcgacgc tacggcgcgc tgaggcgctc aaatcgagat
     6061 aatcagccag ttcgtcgaga gtgtagggtt cctcgcgcca ttccacttcc tgccattcag
     6121 ggtggcacgg atttttgcag atcaggccgc tgtaattcac atccgcgccc agtttttcac
     6181 acaacgcacg ctcaatagcg gcagcgtatc tgagcgccga agccgatgca tccggcgcag
     6241 ttctcacagg aagggcgagc gcgtagagca gatgcgcgtg cccgttacgg ggatttttta
     6301 cggtgatgtt cggggccgga caattccggt cgctccagtc gattgatgcc cccgtccggt
     6361 ccacgtcaaa gggaagccag tacagtgcat gtggctggtt ctgctggatg aaacgggcaa
     6421 gaatggcaat gtttttcggc agaatgcgaa cgccgtacgc gaaatcgttc gtacagtacg
     6481 gtttgtgcgg taatttttcc ctgaagcgtt gaagtacggc gctcatgtga tacgatttct
     6541 ctcggtacac agtttaacta accgcgcttt cccatcggga aacgcggaac ccggcgccct
     6601 gtaatttcgt gatcaatctt ggcggtgaga gcacgattta cggggcgttt gtttttcagt
     6661 cgcttacact acacccaacg gcaaatcctg tcgatccttc ccgaaaaccc gatccattta
     6721 ccataactcc cgttgtacgt accccttaaa acaccctcaa tcgctttctg acgc
//
LOCUS       DAJVYM010000177         6725 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVYM010000177 DAJVYM010000000
VERSION     DAJVYM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.179"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            406..1281
                     /locus_tag="OQS60_003535"
     CDS             406..1281
                     /locus_tag="OQS60_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS2389208.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1289..2065
                     /locus_tag="OQS60_003536"
     CDS             1289..2065
                     /locus_tag="OQS60_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS2389209.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2234..4495
                     /locus_tag="OQS60_003537"
     CDS             2234..4495
                     /locus_tag="OQS60_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS2389210.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4564..5739
                     /gene="senB"
                     /locus_tag="OQS60_003538"
     CDS             4564..5739
                     /gene="senB"
                     /locus_tag="OQS60_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS2389211.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6265..>6725)
                     /locus_tag="OQS60_003539"
     CDS             complement(6265..>6725)
                     /locus_tag="OQS60_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCS2389212.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         1902 a         1558 c         1543 g         1722 t
ORIGIN      
        1 tttcgcacag tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct
       61 caccccccga taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga
      121 catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg
      181 atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat
      241 agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat
      301 tctttttgtt ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat
      361 gttttgtcaa taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc
      421 cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc
      481 ataaagcagg atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg
      541 cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat
      601 cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt
      661 caaaatttgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag
      721 caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat
      781 agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac
      841 atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag
      901 cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct
      961 gtggtacaag aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc
     1021 cagcaactga cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag
     1081 cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc
     1141 atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg
     1201 cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg
     1261 ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt
     1321 aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt
     1381 tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca
     1441 atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca
     1501 acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt
     1561 cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga
     1621 aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac
     1681 agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc
     1741 ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa
     1801 aggccaccta aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg
     1861 cacagccgtc gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga
     1921 aatctcttca ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca
     1981 accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc
     2041 gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagctca tttaactgaa
     2101 atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca
     2161 aaaagacagt agaacataat taataaacaa atagttatac ttatctttat taatattata
     2221 cccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag
     2281 ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc
     2341 agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga
     2401 gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac
     2461 agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt
     2521 gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca
     2581 gagtttttac ggaacctcta cctccggaac aacgacgcat ggttcaacta acaatatggc
     2641 tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag
     2701 tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga
     2761 cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa
     2821 tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac
     2881 gggaagcatt ggcgttatgg ctgctgtgag cggcagttct gtgtactcta atttctcaaa
     2941 tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa
     3001 atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc
     3061 tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa
     3121 atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg
     3181 cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa
     3241 tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgacct
     3301 ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc
     3361 ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg
     3421 ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc
     3481 agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc
     3541 acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa
     3601 cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag
     3661 caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc
     3721 catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga
     3781 taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag
     3841 caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg
     3901 cagtatgcct gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa
     3961 tatgtacatc tacgttaact cggcaagcga cgttatcgca aagggcttcg aactggagat
     4021 ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca
     4081 gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa
     4141 atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat
     4201 cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac
     4261 cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac
     4321 ttacgagtac aaccgcaacc taacactgaa actttcggta caaaacctga tgaacagaga
     4381 ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg agacccaccc
     4441 agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca
     4501 atttccgcta ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga
     4561 aaaatgaata ttttcacttt atccaaagca ccgctatacc tgttaatttc actattttta
     4621 cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa
     4681 acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt
     4741 aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg
     4801 tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca
     4861 ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg
     4921 tccctgcgta tcagtatgtt tgacacccca accggcaaac tggtaagaac cagcgacagg
     4981 ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcattttcg ccatatggtt
     5041 tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag
     5101 ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc
     5161 gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag
     5221 aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg
     5281 ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg
     5341 gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt
     5401 acaaaagatg gactctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac
     5461 acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc
     5521 aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa
     5581 tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac
     5641 attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct
     5701 aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cggggggaaa aaacatggga
     5761 taaaaactcc gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg
     5821 ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca
     5881 cagcattgta cgatcaactg atttgtgcca ataattaaat gaaatcacag ctatgcctgt
     5941 tcatggggtt ctaggccgga accccagatt tttccgattg tttgttttct aactgattat
     6001 tcagtagttt tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac
     6061 tatgataaaa gtcaaagtag gggtgagttt ggatatggaa attattgtac tataaggaga
     6121 gcatgaaaac ccctggggca gtcactgaat gagggaccag cagcggtaaa ataaccagca
     6181 taatttctgc atggtcgatt tttataccgt gacaggctgt tttttatact tggggtcagt
     6241 ttggatatcg aaaattattg tacgttaagg ttgttttttg accttgtcga tcttaactga
     6301 tctcaaacga cgaaacttac ctgaagtcgg tatccggctg tttttccaga tataatcgcc
     6361 tgtcagattg atatgttccc aacctaatgg tgataaatgc gataatagtt gagcatttat
     6421 tttcatgccc ttacgcttca gagcatcaac cgctctttcc atgtaaactg tgttccataa
     6481 ggaaatagcc gccgtcaata aagtcaatcc actggcgcga taactttgat tttccggttt
     6541 ccgatctcta atctccccca atcgatgcat gaatacagca cgggccagag cattacgggc
     6601 ttctcctttg ttcagcccgg cctggacacg tcgccgcaat gccggatccc gaaaccagtc
     6661 cagcataaac agtgttcgtt ctatacggcc aatctctctc agagctttag ccaacccatt
     6721 ttgtt
//
LOCUS       DAJVYM010000178         6647 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVYM010000178 DAJVYM010000000
VERSION     DAJVYM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.180"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..737)
                     /locus_tag="OQS60_003540"
     CDS             complement(<1..737)
                     /locus_tag="OQS60_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS2389213.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="OQS60_003541"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="OQS60_003541"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS2389214.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="OQS60_003542"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="OQS60_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS2389215.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="OQS60_003543"
     CDS             4397..4678
                     /locus_tag="OQS60_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS2389216.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4921..5463
                     /locus_tag="OQS60_003544"
     CDS             4921..5463
                     /locus_tag="OQS60_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2389217.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5543..6217
                     /locus_tag="OQS60_003545"
     CDS             5543..6217
                     /locus_tag="OQS60_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2389218.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6233..>6647
                     /locus_tag="OQS60_003546"
     CDS             6233..>6647
                     /locus_tag="OQS60_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2389219.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1755 a         1507 c         1594 g         1791 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 agatgcgata aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat
     4801 ttgtttcaaa taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat
     4861 ttgtttccaa gagatacaga ctcttaacaa ccaaaaaaaa attttattaa ggaacatatt
     4921 atgaaacgtt caattattgc tgccgctgtc ttttcttctt tttttatgag cgctggagta
     4981 tttgctgcag acgttgatac cggaacatta actattaagg ggaatattgc agaatctccg
     5041 tgtaaattcg aagcgggtgg tgattcagta agtattaata tgccgactgt accaaccact
     5101 gtctttgaag gtaaagctaa atattctacc tatgatgatg cagtcggtgt aaccagcagc
     5161 atgttaaaaa ttagctgccc gaaagaagtt gctggtgtaa aactctcgtt gattaccaac
     5221 gataaaataa ccggtaacga taaggcgata gccagtagca acgataccgt gggttactat
     5281 ctctatttag gtgataacag cgatgtcctg gatgtttctg caccttttaa cattgagagt
     5341 tataaaacag cggaaggtca atatgctatt ccgtttaaag caaaatacct gaaactgaca
     5401 gataactcag tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat
     5461 taataaataa aattgtaagt ttctccaggt aataaacatg gccgctatcc catggcggcc
     5521 ttttaattta agaggcatta aaatgaaagg attattatct ttactcattt tttctatggt
     5581 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
     5641 taaagaagtg atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg
     5701 gattgatgat ggcgatacgt cattaccacc agaaaaaatt caggttcctt tcatgttaac
     5761 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
     5821 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc
     5881 aaatagtcca gagcaagaag gtaagaatgc actgaagttt gcgatgcaaa acagaattaa
     5941 gttgttttac cgaccagcgg gtattgctcc ggtaaataaa gcgacattta aaaaattgct
     6001 ggtaaatcgc agtggcaatg gtttggtgat aaaaaatgac tcagctaatt gggtgacgat
     6061 ttcggatgtc aaagctaata atgtcaaagt caattatgaa actattatga ttgccccctt
     6121 agaaagtcag agtgttaatg tcaaaagtaa taatgcaaat aactggcatc tgaccattat
     6181 cgatgaccat ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     6241 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     6301 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     6361 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     6421 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     6481 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     6541 atgtttaaca tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt
     6601 tcgtctcgat ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaa
//
LOCUS       DAJVYM010000179         6587 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVYM010000179 DAJVYM010000000
VERSION     DAJVYM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.181"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..353)
                     /gene="rnpB"
                     /locus_tag="OQS60_003547"
     ncRNA           complement(<1..353)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQS60_003547"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(386..1531)
                     /gene="garK"
                     /locus_tag="OQS60_003548"
     CDS             complement(386..1531)
                     /gene="garK"
                     /locus_tag="OQS60_003548"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS2389220.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1628..2518)
                     /gene="garR"
                     /locus_tag="OQS60_003549"
     CDS             complement(1628..2518)
                     /gene="garR"
                     /locus_tag="OQS60_003549"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS2389221.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2548..3318)
                     /gene="garL"
                     /locus_tag="OQS60_003550"
     CDS             complement(2548..3318)
                     /gene="garL"
                     /locus_tag="OQS60_003550"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS2389222.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3334..4668)
                     /gene="garP"
                     /locus_tag="OQS60_003551"
     CDS             complement(3334..4668)
                     /gene="garP"
                     /locus_tag="OQS60_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS2389223.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5043..>6587
                     /gene="garD"
                     /locus_tag="OQS60_003552"
     CDS             5043..>6587
                     /gene="garD"
                     /locus_tag="OQS60_003552"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS2389224.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLA"
BASE COUNT         1606 a         1811 c         1634 g         1536 t
ORIGIN      
        1 gttctgtcgt ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta
       61 cccgggttca gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt
      121 ttaccgtgcc acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct
      181 tacctgatcc cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt
      241 ttccccccca ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctccg
      301 cccgtctccc ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc
      361 agtatagagg gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg
      421 cggcgatatt acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca
      481 acgtacctat gctggtcaat acgctgaaga ccgcatcgat gccatgctga tgtacaacgc
      541 caacatcatc ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca
      601 cgtttgcaac gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac
      661 cggtgatcac cagcgtacaa tcgtgaatat gttcttccag attcagcgcc gtagtgacga
      721 tttcaatacc acttttcagt tccgcaccaa gaaacgccat cagcgccgcg cccataccgc
      781 ccgcagctcc tgcgccgggg acatctttca catcaacatg cagcgctttt ttaatgacat
      841 cggcatagtg agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt
      901 gtgggccaaa gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag
      961 cgacgcgaat gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca
     1021 gagtattaag actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg
     1081 cccccagcgc ctgtaccatg cctgcgccgc catcatttgt agcgctgccg ccaatgccga
     1141 taataatgtt tgttgcactg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg
     1201 aagtggttac gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg
     1261 ccatttcaat aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct
     1321 cgcccagcgg ccctgtaacc caggcgtgac gttcagcccc ctgggtggct gcaatcatcg
     1381 cttccaccgt tccttcacca ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa
     1441 aaatttcccg aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt
     1501 tataagagtc tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga
     1561 gggcgtttct cttattcggc ctagattcca ggcccggatt gcaatacacc atccgggcac
     1621 gacgtcatta acgagtaact tcgactttcg ccagtttttc gtagtagcac gccagggcgc
     1681 tatgatccgc cgttcctaaa ccatctgcac gcagtgcctg catcatctcc ataaccgcag
     1741 ctgtgagcag cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc gccagatcct
     1801 taatatgcag atcaatgcgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt
     1861 tggcatccag cacggtactg cccgccagcc caccgcgaat tgcctgataa accaggtccg
     1921 ggttaacgcc cgctttggtt gccagcgtta acgcttctga catcgcagca atattcagcg
     1981 ccacaatgac ctgatttgcc agtttggtga cgttacctgc accgatttcc ccggtatgca
     2041 ccacggaacc cgccatcgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc
     2101 cgcccaccat cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag
     2161 catccagcat atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg
     2221 gtgcgataga actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat
     2281 tctcacccag cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt
     2341 cgcactgttc agcgatcgct ttagccgtag acgctgtttc tgcacctgca gcaatcacgt
     2401 cagcaatagc ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt
     2461 ttttactcat tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat
     2521 caaccatctc tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttctgagt
     2581 ggcagagcgg aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc cccattccag
     2641 ataacgacgc gcatcggctt cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct
     2701 ggcacggtta aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg cattgccgag
     2761 atggcccaat gccgcagcca gatcgctggg gccgacgaag ataccgtcta caccttcggt
     2821 agcggcaata gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt
     2881 aatgttcttg ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggtgcggtg
     2941 agaaacggag acgccgcgaa tgccttccgg cgggtaacga gttgatgcca ccgcctgctc
     3001 tgcttcctct tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag
     3061 acgcttaata attaccggct cgttggtcgg cactcgcact actggcgcgc tggcgctgcc
     3121 tttcagggcc attaactgcg gaataaacgt ggaaatatcg tttggcgcat gttcgccatc
     3181 cagcaccagc cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag
     3241 tgctgaccag caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg
     3301 gaaaacatcg ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat
     3361 gtcgccaact acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac
     3421 cagtgctgca ttgaaggagt gcagttcact taccaggtag ccaatcacca gtggagtgac
     3481 aatggaggca acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt
     3541 cggcgcggtg tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc
     3601 cagcgccatc agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt
     3661 ggaaaccagc aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc
     3721 gcgtttgatc agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag
     3781 tgccggaatc gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag
     3841 ataaatcggg aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa
     3901 aaatacgccc aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc
     3961 gcttgctgcc gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga
     4021 gataaacttc agctcttccg cagacatacg tgggtgatct gtcgggttat gaatcaactt
     4081 gatccacagc gccgtcagca caaaaccaat cccccccata acggtgaaga cgtgctccca
     4141 gccccaagcg aaagtcagcc agccaagcag cggcgaaaag agcgccagcg agaaatattg
     4201 cgccgagtta aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac
     4261 aatacgggcg ttcgccggga atgatggcgc ttccgagaac cccagcataa agcgcataaa
     4321 gaacatggag atccctgccc aggccagcgg gaacatatca acaaagcctt gcaggaaggt
     4381 gaacagcgac cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag
     4441 caaccagccg ccggggattt gcatcagcaa gtaggcccag ccaaaagcag agaagatgta
     4501 acccatcgaa accgcactta actgcaactc ttttgccact tcggtaccag caatagacag
     4561 cgttgcacga tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata
     4621 gctggtatgc acgcctttct tttttacgtc aacggtgtcc agaatcattt tatttacctc
     4681 gggtacttat gctgattttt attattatgg ggaaggtgtt atttatgagt tttatttatg
     4741 ccgttacgac aatgaactcg ggaattagta taagcagcgg gagaataata atcattgtgc
     4801 aaatgctaat ttaatcagta ctatttaaat attattttga gcatatgcac ataaggttgc
     4861 gcgctaaagc acagatttgc gctttacctt accgggcggc actgcaatcc ctgaaatgat
     4921 tgacattgat cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaagtc
     4981 atttattttt aaatgagacc agatcctcat tttaataacc cctggctgga gaatattgca
     5041 caatggccaa catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg
     5101 acacagataa tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc
     5161 cggatgggct ggaattaatt gaacatactc cccaggggca taaagtcgca ttgctggaca
     5221 ttccagctaa tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa
     5281 tcccacgcgg aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac
     5341 acacgctgcc actggcaacc aaagtcccgg cccccttacc gccgctggaa ggatacacct
     5401 ttgagggcta tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca
     5461 ccagcgtcca ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc
     5521 tgctaccgaa atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggctgtg
     5581 gcgtggcgat taacgcaccg gcggcagttg tgcctattcg taccatccac aatatttcgc
     5641 tgaaccctaa ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc
     5701 ctgagcgcct gctggtcggc acggatgatg tgcaagctat tccagtagaa agcgccagca
     5761 ttgtcagttt gcaggatgaa aagcatgtcg gctttcagtc catggtcgag gatattttgc
     5821 aggtcgccga acgccatcta caaaaactga atcaacggca gcgagaaacc tgcccggctt
     5881 cagaactggt cgttggtgtg cagtgcggtg gcagcgatgc gttttctggt gtaacggcaa
     5941 acccggcggt tggctatgcg tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt
     6001 cagaagtaac ggaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag
     6061 aggtcggcaa acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa
     6121 aaaccgaccg cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtgg
     6181 tggagaaggc actcggctcc attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt
     6241 cgcccggtca acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt
     6301 ttgtctgtgg cacgcaacag gtggcttcgg gtatcaccgt acaagtgttt acgaccggcc
     6361 gtggtacgcc gtacggcctg atggcggtac ccgtaattaa aatggcaacc cgcaccgagc
     6421 tggcgaaccg ctggtttgat ttaatggata ttaacgcggg cactatcgct accggcgaag
     6481 agagcattga agaggtgggc tggaagttgt tccactttat tctcgacgtc gccagcggga
     6541 agaagaaaac cttctcggat caatgggggc tgcataacca gctggcg
//
LOCUS       DAJVYM010000180         6532 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVYM010000180 DAJVYM010000000
VERSION     DAJVYM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.182"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..906)
                     /locus_tag="OQS60_003553"
     CDS             complement(<1..906)
                     /locus_tag="OQS60_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCS2389225.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="OQS60_003554"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="OQS60_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS2389226.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="OQS60_003555"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="OQS60_003555"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS2389227.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="OQS60_003556"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="OQS60_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS2389228.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OQS60_003557"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OQS60_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS2389229.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6532
                     /locus_tag="OQS60_003558"
                     /pseudo
     CDS             6387..6532
                     /locus_tag="OQS60_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga ca
//
LOCUS       DAJVYM010000181         6305 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVYM010000181 DAJVYM010000000
VERSION     DAJVYM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.183"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="OQS60_003559"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="OQS60_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS2389230.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="OQS60_003560"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="OQS60_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="OQS60_003561"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="OQS60_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="OQS60_003562"
     CDS             complement(5959..>6305)
                     /locus_tag="OQS60_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2389231.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1571 a         1408 c         1412 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga tagcatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAJVYM010000182         6253 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVYM010000182 DAJVYM010000000
VERSION     DAJVYM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.184"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            122..952
                     /locus_tag="OQS60_003563"
     CDS             122..952
                     /locus_tag="OQS60_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCS2389232.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OQS60_003564"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OQS60_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS2389233.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="OQS60_003565"
     CDS             complement(2081..3541)
                     /locus_tag="OQS60_003565"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS2389234.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OQS60_003566"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OQS60_003566"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS2389235.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6253
                     /gene="gntP"
                     /locus_tag="OQS60_003567"
     CDS             5146..>6253
                     /gene="gntP"
                     /locus_tag="OQS60_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS2389236.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIIS"
BASE COUNT         1480 a         1618 c         1587 g         1568 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtg
//
LOCUS       DAJVYM010000183         6158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVYM010000183 DAJVYM010000000
VERSION     DAJVYM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.185"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            357..982
                     /gene="gstB"
                     /locus_tag="OQS60_003568"
                     /pseudo
     CDS             357..982
                     /gene="gstB"
                     /locus_tag="OQS60_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(979..2094)
                     /gene="yliI"
                     /locus_tag="OQS60_003569"
     CDS             complement(979..2094)
                     /gene="yliI"
                     /locus_tag="OQS60_003569"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS2389237.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2205..2588)
                     /gene="bssR"
                     /locus_tag="OQS60_003570"
     CDS             complement(2205..2588)
                     /gene="bssR"
                     /locus_tag="OQS60_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS2389238.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2801..4126
                     /gene="rimO"
                     /locus_tag="OQS60_003571"
     CDS             2801..4126
                     /gene="rimO"
                     /locus_tag="OQS60_003571"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS2389239.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4173..5494)
                     /locus_tag="OQS60_003572"
                     /pseudo
     CDS             complement(4173..5494)
                     /locus_tag="OQS60_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5502..>6158)
                     /locus_tag="OQS60_003573"
     CDS             complement(5502..>6158)
                     /locus_tag="OQS60_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS2389240.1"
                     /translation="CRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHF
                     SQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINE
                     HFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVR
                     IIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE
                     "
BASE COUNT         1632 a         1542 c         1424 g         1560 t
ORIGIN      
        1 acgctcggcg cttcaacggt ttgttccgcc gcgaatgccg ttggggcaaa aaggaataaa
       61 aatgcagaac ccgctgcaag accacgaagg agagaggagt attgcgtcat aaatgccacc
      121 cgagtatcca ttcaaaaaaa atacgttata tcgccgtatg ataagttcgg tgattaataa
      181 cgcacacaca aacttaaaga aaccgtaagt tggtaaagtt tttaaagttt atgcaatatc
      241 ctgccgcttc gctgcttctg gtgtcatttt tttgccagct gttgcttaaa agttaacttt
      301 acttcaccat cggagtcctg ttcagcctgt ggctgcctgt cggaaaaggg gtgagtatga
      361 ttacgctgtg gggtcggaat aattcaacta acgtaaaaaa gtattgctga cgctcgaaga
      421 actggaacta ccttatgagc aaattctcgc gggccgtgag tttgggataa atcacgatgc
      481 tgattttctg gcgatgaacc ctaacgggct ggtgccgttg ttgcgtgacg acgaaagtga
      541 tctcattctt tgggaatcaa acgccattgt ccgctatctg gcggcacagt acgggcaaaa
      601 acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg gaaaaatgga tggactgggc
      661 aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg ggattagtca gaacaccacc
      721 ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa gagtgcgacg ccctgtttgc
      781 cctgctcgat gcggaactgg caaaagtaaa atggttctct ggcgacgagt ttggtgtggg
      841 cgatatcgct atcgcaccgt tcatctacaa tttgtttaac gttggcctga cctggacacc
      901 gcgtccaaat ctgcaacgct ggtatcagca actcactgaa cgccccgcag tgcgcaaagt
      961 ggtgatgatt cccgttagct aattgcgtgg gctaacttta agtaattccc cactggactc
     1021 gtcggtgaga acgtataaat aaccgtcggg tccagtgcga acatcacgaa ttcgctgccc
     1081 tctgtccgtt aaaatacggc catcttctgt cactttgtcg ccgttgacgc tcatcacaat
     1141 gacatcttta tctttcagcg cgccaataaa taatttttgc tgccactggg ggaatttatc
     1201 gctgttatag aaggccatgc cgctcacagc gggcgaatct ttccagtaaa aaacaggttg
     1261 ctcggtcccg gcgacgatct cccctttcgc ttccggtatc ttaaagcctg aatagttgat
     1321 tccccaggtt gccagcggcc agccgtagtg tttgcctttt tgcgggatat taatttcatc
     1381 accaccgcgc gggccatgtt cattcagcca cagtgcatta ctccacggat tcatcgccat
     1441 tccttgcgga ttacgaatgc cataagacca gatctcggcg cgcgcaccgg attcctttat
     1501 aaaaggatta tcatccggga tttcgccctg gtcggtcaga cgcaccagtt tgccctgtaa
     1561 tttatccaga tcctgcgccg tcgggcgctg attgttttcg cccagagcaa taaaaagata
     1621 acctttaccg tcgaatacca gccgcccgcc aaaatggttg ccggtagaca gttttggcat
     1681 ctggcggaag acggtgcgga agtcggtcac ttttgagaga tcatcactta agcggccata
     1741 acccacagca gttccggctt tgccatcatc gccaacttcg gaataactta accagatgcg
     1801 gcgagactga gcaaaatcag gcgctaaaac cacgtccagc aggccgccct gcccgtgcgc
     1861 ccaaacgtcc ggaactccgg aaagcggcgc agataatcct tttcctgctt gccagtgacg
     1921 caactcgccg ccgcgcagag tgattaacat accgtgatta tcgggtaaaa aggccagtgc
     1981 ccagggatgg tcgagtttgt cttgcagtac ttcgacattt accgttgcag gagccgccca
     2041 gagagcggaa gaaagacaaa taaggggcac aaggaaaaag gattgtcgat gcataacgcc
     2101 tccggattga ctctggctta aagcgtagtc agtggaggag ataacaaatt catttttaca
     2161 aaaacttaaa catgaagggg gagacgcttt ctccccctta gttttcaggc cttctcaagc
     2221 atggcgtgct tctgcaggct ctggatactc agcgttaagc tcatcagaca attttcaagc
     2281 ttatcggcgt tgacggtaat aacagtcggg caatcatggt gcccactcat caaacatact
     2341 gcggctgtcg ctaatgcttc ttcagcatga tgaagagcac tccactcttc ctgatccaga
     2401 tgaagattca accgcagcga tttatcgtgc agttcgcgat tcagtttaaa aaagttatct
     2461 cgtagatgat tgctttcgct gacggacatg tatccttttg ccttcctcag ttgcacgtat
     2521 gcagcgaggt caacgcgtgc gtcgatcaac cggttcagca gatcgattcg ctgtctgtca
     2581 acgaacatac ttcgatcctc ctctttccag ccttgttgcg attaaatagt atggctattt
     2641 tttgagcaaa atgctgtcca ggatcaattc ttcacgccaa attaacaaaa tttacatttc
     2701 cttcgcctcc gccagcgaat gagattgcga tacaaatttt atccagaact ctgtagaatc
     2761 cctgccctga cctttcctgt aactgaatat tttttaagct atgagcaaag taactcccca
     2821 gccgaaaatc ggctttgttt cccttggctg tccgaaaaac cttgtcgatt cagagcgtat
     2881 tctcaccgaa ctacgcactg aaggttatga cgtggtaccg agctatgacg atgcggacat
     2941 ggtgatcgtc aacacctgcg gctttattga cagcgcggta caagaatcac tggaagccat
     3001 tggtgaagcg ttgaatgaaa acggcaaggt aattgtgacc ggttgtctgg gggcaaaaga
     3061 agatcagatc cgcgaagtcc acccgaaagt gctggaaatc accgggcctc atagctatga
     3121 gcaggttctg gagcacgttc atcactacgt gccaaaaccg aaacacaacc cattcctgag
     3181 cctggtgcca gaacaaggtg tgaaactgac accgcgtcat tatgcctatc tgaaaatttc
     3241 ggaaggctgt aatcaccgct gcaccttctg cattattccg tctatgcgcg gcgacctggt
     3301 gagccgtccg attggcgaag tgttaagtga agcgaaacgt ctggtagatg cgggcgttaa
     3361 agagattctg gtgatctcgc aggatacttc cgcctatggc gttgatgtta aacatcgtac
     3421 tggcttccac aacggcgagc cggtaaaaac cagcatggtc agcctgtgcg aacagttatc
     3481 gaaactgggg atctggacac gtctgcacta cgtttaccct tatccgcatg tggacgacgt
     3541 catcccactg atggcagaag gcaaaatcct gccgtatctg gacattccgt tgcagcacgc
     3601 cagcccgcgc attctcaaac tgatgaagcg tccgggttct gtagatcgcc aactggcgcg
     3661 catcaaacag tggcgcgaaa tctgcccgga actgacccta cgctcaacct ttattgtcgg
     3721 cttccctagc gagacggaag aagatttcca gatgctactc gacttcctga aagaagcgcg
     3781 tctggatcgc gttggctgct ttaaatacag cccggttgaa ggtgcagacg ccaatgccct
     3841 gcctgaccag gttccggaag aagtgaaaga agaacgctgg aaccgtttca tgcagctgca
     3901 gcagcagatt tccgccgagc gcctgcaaga gaaagtgggc cgtgaaattc tggtgattat
     3961 cgacgaagtg gacgaagaag gcgcgattgg tcgcagcatg gcagatgcac cggaaatcga
     4021 cggcgcggtt tatctcaatg gtgaaaccaa cgttaagccg ggtgatatcc tgcgtgtgaa
     4081 agtcgagcac gccgatgagt acgatttgtg gggtagccgg gtttaattaa ttcaggccgg
     4141 gcaaaatcat ttgtccgccc tgcgaataat gattactgcc agctaatctg agccctgccg
     4201 tttttcttac tcttatacag ccgctcatcc gccagcattt tcaagcgttc gacggtggta
     4261 cattcatttg aatgcgctac accgatactg gtacttaaat taatgcttcc cacctggaaa
     4321 tgaacgattt cttttagaat cgatagcaac tcttccagcg ttccacccgt ataaatcacc
     4381 gcaaattcgt caccgccgaa gcggtagatt ttacctttat ctaccgggat ctgtgattta
     4441 aggattttca ccactttcat caacacttca tcgcccagca aatgaccgaa ggtgtcgtta
     4501 atgtttttga atttatcgac gtcaaacagg cacaaataac cgcctttttc cggcagcgct
     4561 tttaaatctt cgtcaaagga ttttctgccc attgctttgg tcaggtcgtc caggaacgcc
     4621 gagttataca tactgtagag ttttttgaca tcggcagccg aaaagaaaag cccgccatcc
     4681 agtacgcgtt caaaatggtt attaatgatg gcttctttct ccacaatacc acgattaatc
     4741 actctgcgta ccagatagag gcaactggta cccgatatca ctgccaccaa tgcggcaaag
     4801 aaaataatca atgtacagac ccgcaaataa acatcttttt tatacgcaaa tacgtacaat
     4861 ccggtcggca aataggcgaa gtgctccatc gcttccgaaa cggtgaagat ggcgttttta
     4921 gcattatatt catcaccggt ttaccgttct taaatagaga gaacatcagg ttctcgctgt
     4981 ccatcgccag cgattttgac agtaaagaaa aacgctccag accaagcaaa aatatcagac
     5041 tacgattgtg agttaccgga tagaggtaat agacgaaggt tttaccgtca aaagagataa
     5101 accctgtatt tttgacatgt cgatacttca caacctgcat aatatctgca caataatttt
     5161 tctcgaactc ctttacctga atacattcgt cactggaaat cagataagga ccgacatcgt
     5221 acaaataaga gggggaaacc gagtcggcgt aagctgtgcg caaaccaacc aacagctgtt
     5281 cctccgtggg atttagcgtg ttgataagat caattgccac gctacgccga tattcagcaa
     5341 tatttgccac cgcaaaagca gtatcaataa cggactgttt caggctatac acccgttcct
     5401 ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat catgataaaa atcagcagac
     5461 tgactgtaag tacgaactta ttgattctgg acatacgtaa actactcttt tactaatttt
     5521 ccacttttat cccaagcgga gaatggcatg ggtttctggt acagataccc ctgagcctga
     5581 acacatccta atttaattaa ttcgcgcgca acgtcttcgc tctctacgcc ttccgcaatc
     5641 accgggatcc ggttatagcg tgccagatcg aaaataatgc gcacaatttt tcccgcagta
     5701 ccgaaaatat cgccggtaaa ttgcgagtcc acctttatgc tgtctggatt aatttcataa
     5761 aaacgtttca ggtttgacaa accggtgcca aagtcatcaa tcgatatccg taccccttgt
     5821 ttacgcagtt gttcaatatt gagataaaaa tgttcattaa ttcgctcgat cacggtttca
     5881 gtgatctcaa cacagaggct gttcgccttc aggccatttt ctttcatgat ggtggtaaaa
     5941 cgctcatgaa agtgcggttc gtttaactgc cagggcgaaa cattaatatg cagtaagaaa
     6001 tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt cacccattgc cgttttaata
     6061 atctgataac ccagctcatt gataaacccg atatcttcag caatggtaat gaacttcagc
     6121 ggtgaaatga tcccccgctc cgcagaaacc caacggca
//
LOCUS       DAJVYM010000184         6096 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVYM010000184 DAJVYM010000000
VERSION     DAJVYM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.186"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(107..4518)
                     /locus_tag="OQS60_003574"
                     /pseudo
     CDS             complement(107..4518)
                     /locus_tag="OQS60_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
     gene            complement(4538..4999)
                     /locus_tag="OQS60_003575"
     CDS             complement(4538..4999)
                     /locus_tag="OQS60_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCS2389241.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..>6096)
                     /gene="tssI"
                     /locus_tag="OQS60_003576"
     CDS             complement(5027..>6096)
                     /gene="tssI"
                     /locus_tag="OQS60_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2389242.1"
                     /translation="GWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASE
                     LHGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEI
                     FCDEHGRVRVKFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNG
                     DPDQPIIMGRTYHQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIH
                     AQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSE
                     DVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGE
                     STVIRLSKDGITIVGGSVFIN"
BASE COUNT         1066 a         1923 c         1664 g         1443 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cttggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggggcggttt tcctggtggt
     5581 aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtct acaatcactt
     5641 cctgacccac acgcggtatc gccaggtggc caaaaccggt gcctgcccac gcctgtgcca
     5701 cacggatcca gcacgaactg tcttggtctg ccgggttata acggtcccag ttgaacttca
     5761 cccgcacgcg accatgttcg tcgcagaaga tttcctcgcc tgccggtcct gtcacgacag
     5821 cgctctgcgg gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg
     5881 ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg
     5941 cctgtggctg ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg
     6001 cctgcggatg ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca
     6061 ttccccgcgc ggtttctgca ttgtttcgcc agccgt
//
LOCUS       DAJVYM010000185         5974 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVYM010000185 DAJVYM010000000
VERSION     DAJVYM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.187"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..202)
                     /locus_tag="OQS60_003577"
     CDS             complement(<1..202)
                     /locus_tag="OQS60_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS2389243.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
     gene            718..1287
                     /locus_tag="OQS60_003578"
     CDS             718..1287
                     /locus_tag="OQS60_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389244.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2014..2187
                     /locus_tag="OQS60_003579"
     CDS             2014..2187
                     /locus_tag="OQS60_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS2389245.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2445..3773)
                     /gene="rutG"
                     /locus_tag="OQS60_003580"
     CDS             complement(2445..3773)
                     /gene="rutG"
                     /locus_tag="OQS60_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS2389246.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3794..4288)
                     /gene="rutF"
                     /locus_tag="OQS60_003581"
     CDS             complement(3794..4288)
                     /gene="rutF"
                     /locus_tag="OQS60_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS2389247.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4299..4889)
                     /locus_tag="OQS60_003582"
     CDS             complement(4299..4889)
                     /locus_tag="OQS60_003582"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS2389248.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4899..5699)
                     /gene="rutD"
                     /locus_tag="OQS60_003583"
     CDS             complement(4899..5699)
                     /gene="rutD"
                     /locus_tag="OQS60_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS2389249.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5707..>5974)
                     /gene="rutC"
                     /locus_tag="OQS60_003584"
     CDS             complement(5707..>5974)
                     /gene="rutC"
                     /locus_tag="OQS60_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS2389250.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1606 a         1537 c         1446 g         1385 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
      301 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
      361 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc ccctcttcaa
      421 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
      481 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      541 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      601 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      661 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      721 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      781 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      841 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      901 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      961 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     1021 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     1081 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     1141 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     1201 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     1261 agccgtcact ggacaaagac actgtaaaaa aacttagtag catcttcata gcctccttca
     1321 tggggacttt tttgtgtctg taacatatgg tgaccaaaca tctatatatc gttatatagc
     1381 cctgtatata gcactatata tagtgtctgg agagggacga ctaacaccct gcatagttcc
     1441 ctgaaaaggg gcttcacaga ttctctatat atttcaataa gttaggataa aaagggacta
     1501 taccgatact gcatagaatc tgtgtaggat tgcatagtca taggggttgg aacaagccca
     1561 actacacctg ataaagcctg ttattaacag gtagttgaat gtacgtgttt agctatgtaa
     1621 aagcaccaga actttagcca tttctaacca ctcctcgtgt tatctctatt ccgtagcgat
     1681 tcgctaccac ttatttaaag ataagacgtc cttttcagag tgcaaatttc acaaccactt
     1741 atttgattta taacaacttt cacaagcacg taattttgtc gcaaaatgac acatttttat
     1801 ctcatcgcgt ttttttaatc ataagagcgg cttatggata attattggag atgatatcta
     1861 ttctcgctaa gaagctgttg caggatatta ccaaacgcgg gtctacccgc gtcagttcac
     1921 taagcttagt cccacgtagc gaaaatatgg cagccgccat acgccgcgtt aattctatgc
     1981 aatatgatgt ctatacccag acggaggtca gtaatggcaa accatcgagg cggttccggc
     2041 aattttgcag aagaccgcga aagagcatca gaagcaggta aaaaaggtgg acagcacagc
     2101 gggggtaatt tcaagaatga cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag
     2161 agcagtcacg gcaaaagcga caactagccg ggctaatcaa tgacgaatgc atttttgtct
     2221 gtagctcgtc aaaaagccat caccgccggt tacccggtgg ttgatactga tgacaaatgt
     2281 aagcttgcct gatgcgcgat gcttatcagg cctaccagaa gattgcaata tattgaattt
     2341 gcactgtttt gtaggccgga taaggcgttt acgccgcatc cggcatgaac aatgcgtacg
     2401 ttgtcaacaa tctgcaccgc cgggtaaccc cggcggtttt ctgtttatgg ctcctgatga
     2461 acaacttctg gcggtggaac gtcaaccaac cttctactta acaacgcatt gagtaaaatc
     2521 gcgccaaagg tggctgtacc gatccctccc aacgtaaaac cgcccagcgt gagagcaaaa
     2581 tcacccgcgc ccagcactaa ggttactgcg accataatca aattaccgtt ctggcttaaa
     2641 tcgacacggt tttgtaccca gatccttgcg cctgcgacgg caatcagccc gaacacaaca
     2701 attgatgcac caccaatcac cgcggccgga atggtatgga tcagcgcgcc aaatttcggc
     2761 gaaaagccca acagcatggc gatgacggca gcagcaacaa acaccagcgt cgagtagact
     2821 tttgtcacgg ccatcacacc gatattttca gcataggtgg tgacgccgct accgccgaca
     2881 gagccggaaa gcatcgttgc cagaccatca ccgacgaatg ctcgccccat atacgggtcc
     2941 atattgcgtc cggtcatccc ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga
     3001 atcaccgcca cgggcgcaat cagcatcatt gcctgactat taaaagcagg agtggaaaaa
     3061 tgtggcaaac cgaaccaggc agcatggctg acgagagtaa aatcgacggc ttttcccagc
     3121 cctaaaacgt tggtcatcac gccatacagc agacaggcga caattaaacc tacgagaatc
     3181 aataaccgct ggatcatgcc acgggtaaac accgccacca gcccaataca cagcaccgtc
     3241 attaccgcca tccagctatc aaaggctgaa gccgatacac ttttcactgc gataggcgct
     3301 aagttcaggc caatcgccat caccaccgca cccgtcacca ccggcggcat tagtcgttca
     3361 atccagcgtg taccaatttt catcaccacc aggccaatga cggtataaac cagcccacag
     3421 gcgataatcc cgcccagtgc gatgctgata ttcgggttaa tgccctgacc gttaaagccc
     3481 gtcgcggcaa tcaccacgcc gacaaaagcc gcgctggagc cgagataact ggggacgcgc
     3541 ccgccggtaa taaagaaaaa cagtaacgtg ccgatccccg acattaaaat ggaaagattg
     3601 ggatccagcc ccatcagaat cggcattaac accgtcgcgc caaacatcgc caccgcgtgt
     3661 tgaacgccca ttactgccgt ctgagcaaac ggcaatcgtt catccggcgc gaccacgccg
     3721 ctctctgtag aggtcgattt taactgccag tgaggaaaac cgaacattgc catcagctgt
     3781 ctccttaagg aggttaacaa gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca
     3841 ccagcccgta gggtgtggtg tgacgatgaa tcgcttcgat ggcgcaaaac agaatgtcgt
     3901 gggtgccgac gctcaccacc tggctgatgc ggcagtcaaa cgaaaccaga gcctcttcca
     3961 gttgcgggca tccggtcaca cccgtctgcc agcgggcggc ggcaaagcgg tgttccatgg
     4021 gcgttttgcc gccaaaaaga tttgaaagcg gctcctgccc ggcgctcagt gtatttacac
     4081 acagcgttcg attttcattg aataccggcc agacggacgc cccacgattc aggcacacca
     4141 gtaatgtggg cggcgtgtcg gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc
     4201 cggatggacc gtccgtggtg atgatattga ccgccgcgcc catacaggac atcgcatcgc
     4261 gaaaggtttg ttgatcaact atgttcatag tttgctcctt acaacagccc gcaggcttct
     4321 tcaaaggaca gacgtggcag gcgcgcaaaa agtttgctgc tatcgccata gccgatatta
     4381 atcagcagat tgctcttcag cgtgctgccc gcaaaaaagg cgtcgtcaac gtgttgacgg
     4441 tcaaaacccg acatcgggcc ggtatccagt cccagcgcac ggcaagcgac gatcagatag
     4501 gccgcctgca tggagctgtt gcgaaacgct gtttcttcgg caagttgtgg gctggaggta
     4561 aaccaactgc gggcatcacc gtggggaaac agtagtggta accgttcata aaattcactg
     4621 tcccaggcaa cgatagcggt gacgggcgcg gtcagggttt tttgtagatt gccgctggaa
     4681 agcgccgggt gcagacgttc ttttccttct acagtgcgga taaacacgat ccgtgccgga
     4741 gaacagttag ctgatgtcgg cccccatttt atcggggcat aaagctcccg taacgtctca
     4801 tcgctgacgg gtgtctcccg ccagccgttg tgagtgcggg catcggtgaa cagggtgcta
     4861 agcgcacctg ggctaacggc ttcgttcata gcaattcctt acagggcggc ttcacggtga
     4921 tgtaacaggc tggcaagccc gttgagtaac agagcattaa acgtttcggg gtcggtcacg
     4981 ttgcaggcgt gtccgccata gcgcatcacc attttctggc tatcgggcag ggcggcatga
     5041 agctcactgg aacatgctga tggcaccagc agatcatcac tggcgcagat gatttgcacc
     5101 gggcagcgga tgcgatccgc atggtgacta aagtcagcgc gtttgagggc gttaagtcga
     5161 cgcagtaaat tatttttgcc ctgaaaatgc gccagtgcca gcgcgtcttc tgcctcaagg
     5221 cgaggtgcgc gggccgccat ccagtcggcg ggatagagga acaacggctg cgcttccacc
     5281 catgcctgcg cgccgccgct atacagtaat cgttcgcgaa cctgaaaaca gcggcgcgta
     5341 tgggcgttta ttcgtagcca gccgttaacg ctgatcagca cagttaccga cgcgggataa
     5401 tccagcgcca gctgcattcc caccagcgca ccgagcgcat ggccgaccac cgcgtaatgc
     5461 tcaatccctg cggctaccag cgcctgatgc agttccgctg ccatctgggc gatactgtaa
     5521 tcttctgcca gcgtgtcggg attattgccg gtgccgcgct ggtcgtaaca gactacctga
     5581 tactcctgct ccagcaccgc cagttgcggt aaccagtaac tgccgctacc gccaagaccc
     5641 gaaatcaaca ccactacggg cgcatcagca taagggggag gtgagagtga aagtttcatc
     5701 gcggcctcac ttggcgatat gcgcaattgt ggcgatttcc accagcgcgt caggttttac
     5761 cagtccgcac tgaatgcaga atcgcgccgg tttatcaccc ggaaaaaact cggcgtagat
     5821 ttcgttaatc gcggcgtaat ttttccagtc ggtaataaag atgctgttga aggtcacatc
     5881 cgccatcgtg ccacccgccg tctcgatcac cttgcggata gtttccagaa cgtggcgggt
     5941 ttgcgccttt gggtcatcgg caaacagcac gtta
//
LOCUS       DAJVYM010000186         5931 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVYM010000186 DAJVYM010000000
VERSION     DAJVYM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.188"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            292..368
                     /locus_tag="OQS60_003585"
     tRNA            292..368
                     /locus_tag="OQS60_003585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="OQS60_003586"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="OQS60_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS2389251.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="OQS60_003587"
     CDS             complement(1357..2247)
                     /locus_tag="OQS60_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS2389252.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OQS60_003588"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OQS60_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCS2389253.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="OQS60_003589"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="OQS60_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HCS2389254.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1347 a         1559 c         1550 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAJVYM010000187         5914 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVYM010000187 DAJVYM010000000
VERSION     DAJVYM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.189"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(42..1046)
                     /gene="cra"
                     /locus_tag="OQS60_003590"
     CDS             complement(42..1046)
                     /gene="cra"
                     /locus_tag="OQS60_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS2389255.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1128..1214)
                     /locus_tag="OQS60_003591"
     CDS             complement(1128..1214)
                     /locus_tag="OQS60_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS2389256.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1226..1717)
                     /gene="ilvN"
                     /locus_tag="OQS60_003592"
     CDS             complement(1226..1717)
                     /gene="ilvN"
                     /locus_tag="OQS60_003592"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS2389257.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1720..3444)
                     /gene="ilvI"
                     /locus_tag="OQS60_003593"
     CDS             complement(1720..3444)
                     /gene="ilvI"
                     /locus_tag="OQS60_003593"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS2389258.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3563..3607
                     /locus_tag="OQS60_003594"
     CDS             3563..3607
                     /locus_tag="OQS60_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389259.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3762..4706)
                     /gene="leuO"
                     /locus_tag="OQS60_003595"
     CDS             complement(3762..4706)
                     /gene="leuO"
                     /locus_tag="OQS60_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS2389260.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5366..5452
                     /gene="leuL"
                     /locus_tag="OQS60_003596"
     CDS             5366..5452
                     /gene="leuL"
                     /locus_tag="OQS60_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS2389261.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5545..>5914
                     /locus_tag="OQS60_003597"
     CDS             5545..>5914
                     /locus_tag="OQS60_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS2389262.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAI"
BASE COUNT         1600 a         1529 c         1322 g         1463 t
ORIGIN      
        1 gccagaggga aattcacctg gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca
       61 ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg
      121 tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc
      181 actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt
      241 gccaggtcag aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac
      301 aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg
      361 aataactggg cagccacctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc
      421 ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc
      481 ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc
      541 atttcggcat catcctgatc ggcaccaacc acgctggtga agtgttcacg atcgagggcg
      601 cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca
      661 ggaggcaacg acgtcgaaac aataatggca tcgacctgac gctgtaaaag gtgctcaata
      721 cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa ttagcagttg ataaccccgt
      781 tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg
      841 gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc
      901 gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt gtcgctcaca
      961 cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc
     1021 agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc
     1081 cattgtactg gtatgggtta aattcattaa catctaagcg cataaaatta actgcggcaa
     1141 ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc
     1201 tgcaaattgc gcattcagat cattctcaac gcattatttt atcgccgcgc gaaagtccga
     1261 ccacaccaga acgagcaact tccacaattt tcgccacctc gcgaatcgat gctaaaaatg
     1321 catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggt gtgacatcga
     1381 taatttgccc acggaatatt tccgtattac gtttcacttc gtcacgcccg taaccgctgg
     1441 cctgaatttt caccagcatg atttcccgct caacatgcgc gccctgcccc aactcactca
     1501 cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat ctgctcaagt actttttcat
     1561 cgcccacggt ctggatggtc atacgcgata atgtcggatc atcggttgac gcaacggtca
     1621 ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg
     1681 attcattttc gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct
     1741 taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcgctgcc
     1801 atcgacggta acatcaacaa acaccaggcg attattgtgc acctgttcca gcgcctcgct
     1861 aagtttgctt tccagctcat gcggatgaga aatctggatc ccgacgtgac cataggcttc
     1921 cgccagacgg acgaaatcgg gtagcgattg catataagat tgtgaatgac ggccggaata
     1981 gatcatgtcc tgccactgct tcaccatccc cagatagcgg ttattgagat tcaccaccag
     2041 tacgggcaac tcgtattgca acgcggtaga cagttcctgg atgttcatct gaatactgcc
     2101 gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc attttgacgc ccagtgccgc
     2161 aggtaaacca aaacccatcg tgccgaggcc accggaattg atccagcgac gcggtttgtc
     2221 gaatggataa taaagtgcag caaacatctg gtgctgcccg acatcggacg tcacgtaagc
     2281 gtctcccttc gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact
     2341 gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatttgct gccaccagtc
     2401 gcggatctca tccagtggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc
     2461 gaggacctgg cgagcatccc ccacaatcgg gatatccgca gtcacggttt tagaaatgga
     2521 agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt
     2581 cgtcgttcgg tcatcaaatc gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt
     2641 catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc
     2701 cggaaacgct cccagcccca tcaatgagga aacaacgggc agattcaacg cctccaccgt
     2761 ttctttcaac tgctgatggc agcccgccgt gattgcccca ccgcctacgt agacaaccgg
     2821 tttttttgcc gctaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt
     2881 agtgggattg taagaacgca tactgaccga ctccggccag acatagggta atttgttcgc
     2941 cggattaaga atatctttcg gtaaatcaac gactactggt ccaggacgac cacttgccgc
     3001 cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact
     3061 gtgtttaacc actggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc
     3121 tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc
     3181 ggtggcgatg ccagtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc
     3241 gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg
     3301 aactaaaaca tgatcgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc
     3361 gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat
     3421 ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa
     3481 acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa acgcagggta
     3541 ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggatt
     3601 aaattgataa taaatcggct gaatcccaca acttacagca atttctctat ttttttaatt
     3661 aatcctaaaa tagagagccc acacattcag aataaaattc tacattcaat aaaaaataca
     3721 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
     3781 actaattgct cttccatcca ctgatggcct ttatcgcgcc cggcagcttc atgccaggag
     3841 agataacagg ttctgctatt ttgttttaac ggcagcggta atacctgtaa ttctaaggat
     3901 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
     3961 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
     4021 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
     4081 tcgttataaa catcatgttt cagtaacggg cccttaattg ttggatgatt tttgctggct
     4141 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
     4201 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
     4261 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
     4321 tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
     4381 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
     4441 actgagccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
     4501 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
     4561 gacataccca gaacatgagc ggcacgggta atgttttgct cctgcatcac ggcatcgaaa
     4621 acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc cgccgtctct
     4681 gaatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
     4741 cttattggaa tgcagagaaa taatcctgaa gaatatgatt tatcatgcat ataaaataag
     4801 aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac gggttctgat aaaaacagaa
     4861 tccattgccg tggaatgagt catttaccac agcataagaa tccataatgt ttttgttgcg
     4921 aaaacaatct aatcataact accgcgaata ctcaatcatc tacaaaatgg attaaatgtg
     4981 cttatttaat aattaattta tatatttaat gcattaattc ttaacattaa ttgatcaata
     5041 atattcacca aatcaatatc aaaaaaaatc gcaaaacata taattcaata caaatcatca
     5101 ggataggttt tgcaacgcgt gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa
     5161 tttatgctga aatatgtcaa ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg
     5221 cttactttaa ccaccgcagc acaattagct aattttacgg atgcagaact cacgctggcg
     5281 ggacgttttt attgcgtcag ggttgacatc cgtttttgta tccagtaact ctaaaagcat
     5341 atcgcattca tctggagctg atttaatgac tcacatcgtt cgctttatcg gtctactact
     5401 actaaacgca tcttctttgc gcggtagacg agtgagcggc atccagcatt aagccagcac
     5461 gcagtcaaac aaaaaacccg cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct
     5521 ctaaaagaga caaggaccta aaccatgagc cagcaagtca ttattttcga taccacattg
     5581 cgcgacggtg aacaggcgtt acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg
     5641 ctggcccttg agcgtatggg tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg
     5701 ggcgattttg aatcggtgca aaccatcgcc cgccaggtta aaaacagccg cgtatgtgcg
     5761 ttagctcgct gcgtggaaaa agatatcgac gtggcggccg aatccctgaa agtcgccgaa
     5821 gccttccgta ttcatacctt tattgctacc tccccaatgc acatcgccac caagctgcgc
     5881 agcaccctgg acgaggtgat cgaacgcgct atct
//
LOCUS       DAJVYM010000188         5864 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVYM010000188 DAJVYM010000000
VERSION     DAJVYM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.190"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            80..430
                     /gene="traA"
                     /locus_tag="OQS60_003598"
                     /pseudo
     CDS             80..430
                     /gene="traA"
                     /locus_tag="OQS60_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            445..756
                     /gene="traL"
                     /locus_tag="OQS60_003599"
     CDS             445..756
                     /gene="traL"
                     /locus_tag="OQS60_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCS2389263.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            778..960
                     /locus_tag="OQS60_003600"
                     /pseudo
     CDS             778..960
                     /locus_tag="OQS60_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            962..2212
                     /locus_tag="OQS60_003601"
                     /pseudo
     CDS             962..2212
                     /locus_tag="OQS60_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2209..2595
                     /gene="trbI"
                     /locus_tag="OQS60_003602"
     CDS             2209..2595
                     /gene="trbI"
                     /locus_tag="OQS60_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCS2389264.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2592..2903
                     /locus_tag="OQS60_003603"
                     /pseudo
     CDS             2592..2903
                     /locus_tag="OQS60_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            2898..3896
                     /locus_tag="OQS60_003604"
                     /pseudo
     CDS             2898..3896
                     /locus_tag="OQS60_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3916..4663
                     /gene="traX"
                     /locus_tag="OQS60_003605"
                     /pseudo
     CDS             3916..4663
                     /gene="traX"
                     /locus_tag="OQS60_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4718..5278
                     /locus_tag="OQS60_003606"
     CDS             4718..5278
                     /locus_tag="OQS60_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS2389265.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5408..5620
                     /locus_tag="OQS60_003607"
     CDS             5408..5620
                     /locus_tag="OQS60_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS2389266.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1379 a         1430 c         1787 g         1268 t
ORIGIN      
        1 tgcttccatc ggaaacgatg atctggcagc ctgagttcac agataaaaca ctctccagga
       61 aacccggggc ggttcagcgt taagtgttca gggtacttct gcgcccgtca aaaagaaatc
      121 gtttttttct aaattcactc gtctgaatat gcttcgcctg gttcgtgcag tgatcccggt
      181 tgctgttctg atgatgttgt tccctgagtt agctatggct gctggtaaag gcacggacct
      241 gatggctaaa ggtcaggaaa cggttaaggc gaccttcggt aaggactcca gtatcgttaa
      301 atgggttgtt ctggctgaag ttctggtcgg tgcagtcatg tacatgatga ccaaaaacgt
      361 caagttcctg gtcggctttg ccatactctc tgtatttatt gcagtaggta tgtctgttgc
      421 cggattctga caggaaataa aacgatgttg ggagacgaga ataaacttaa gaaatatcgt
      481 ttcccggaaa cactgaccaa ccagagccgc tggtttggcc tgccactgga tgaactgatc
      541 cccgcagcaa tctgtgttgg ctggggtatc acaacatcga aatatctgtt cggtattggt
      601 gcagcggttc tggtttattt cgggattaaa aaactgaaaa aagggcgggg cagttcctgg
      661 ttacgtgacc tgatttactg gtatatgcca acagccctgc tgcgcggtat ttttcataat
      721 gttcccgatt cgtgtttccg gcagtggatt aaatagaact gataccagga ttgttatatg
      781 gaacacggtg cccgtttaag taccagtcgt gtaatggcca tcgcctttat atttatgtca
      841 gtgcttattg ttctcagcct ctctgttaac ctcattcagg gggtgaataa ctaccgtctt
      901 cagaatgagc aacgcactgc cgtgacccca atgggattta atgtgccctt tgccgtgtca
      961 cacctaccgt aaccagctgg cgtttatcga tattttcttc cggggaatga ataacaccaa
     1021 ctacaacatg gcggtctgtg gcacctccgg ggccggtaaa accgggctga tacagccact
     1081 tatccgcagc gtgctggact ccgggggctt tgccgtggtg ttcgacatgg gggatggcta
     1141 caagtccctg tgtgagaaca tgggtggcgt atatctggac ggtgaaaccc tgcgttttaa
     1201 cccgtttgcg aacatcaccg atatcgacca gtcagcggag cgtgtccgtg accagttgtc
     1261 agtgatggcc agccccaacg gtaacctgga tgaagtgcat gaaggtctgc tgctgcaggc
     1321 ggtcagggcc tcctggctgg ccaaagagaa cagggcacgt attgatgacg tggtggattt
     1381 cctgaaaaac gccagtgaca gcgagcagta tgccgggtca ccgactatcc gcagccgtct
     1441 ggacgaaatg attgtgctgc ttgaccagta cactgccaac ggcacttacg gccagtattt
     1501 taactctgat gagccgtccc tgcgggatga cgcaaaaatg gtggtgctgg agctgggcgg
     1561 actggaagac cgtccgtcac tgctggttgc ggtgatgttc tccctgatta tctacatcga
     1621 gaacaggatg taccgcacgc cgcgtaacct caagaaactg aacgtcattg atgaaggctg
     1681 gcgtctgctg gacttcaaaa atcacaaggt cggtgagttt attgagaaag gttaccgtac
     1741 cgcccgtcgt cataccggtg cttatatcac catcacacag aacatcgtcg actttgactc
     1801 tgacaaggcc tccagtgcgg cccgtgcggc atggggtaac tcctcctaca aaattatcct
     1861 caaacagagt gcgaaggagt tcgcaaaata caaccagctg tatccggacc agttccagcc
     1921 actgcagcgc gacatgattg gtaagtttgg tgcggccaaa gaccagtggt tcagttcctt
     1981 cctgctgcag gtggaaaacc attcctcctg gcaccgcctg tttgtggacc cgttaagccg
     2041 cgccatgtac agctctgacg gcccggattt tgagtttgtg cagcagaaac gtaaagaggg
     2101 gctgagtatt catgaggcgg tgtggcagct ggcgtggaag aagtcagggc cggagatggc
     2161 ttcgctggaa gcctggctgg aagaacatga gaaatacagg agtgttgcat gaccacaacg
     2221 caaaaaacga ctgatgttac agccccccga cgcagccact ggtggtggac ggtgccggga
     2281 tgcctggcaa tggtgttact gaacgcagcc gtcagttacg gcattgtcag actgaacgca
     2341 ccgatgactg ccgccttcaa catgaaacag actgtggatg cattttttga cagcgccagt
     2401 cagaaacaac tgtcagaagc acagtcaaaa gccctttcag cgcgttttaa cacggcactg
     2461 gaagccagcc tgcaggcgtg gcagcagaaa caccacgcgg tcattctggt gtcgcctgcc
     2521 gtggtacagg gggctccgga tatcacccgg gaaatccagc aggatattgc ccggcgaatg
     2581 agagcggaac catgagatgc cgggggctga ttgccctgct gatatggggg cagagtgtgg
     2641 ccgccgccga tcttggcacc tggggtgatc tgtggccggt aaaggagccg gacatgctga
     2701 cggtgatcat gcagcgcctg acggcactgg agcagtccgg tgagatgggc cggaaaatgg
     2761 atgcctttaa ggagcgggtg atccgcaaca gtctgcggcc ccctgccgtg cccggtatcg
     2821 gacgcacgga gaaatacagc agccggttgt ttgacccgtc cgtcagactg gctgcggata
     2881 tccgggataa cgaagggcgg gtggccggct ttgagtcagc ctacgtggcc ctgtcgcgta
     2941 tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat gccatcaaca
     3001 atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac cgggaggtca
     3061 tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg gcaggccgtg
     3121 ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt attgctccgg
     3181 gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac ggcaagtccg
     3241 ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg ggattcagcg
     3301 gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag ggcagccgta
     3361 acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc cgtgataatc
     3421 ctgacagtgg tgtggtggta agcattgccg gtgaaggccg tccgtggaat cccggtgcca
     3481 tcaccgatgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg ggagccggaa
     3541 atggcgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag gccatccgcc
     3601 gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac aaacctgacc
     3661 tgccggacgg caaaacagag ctggctgtca gggaaattgc cgggcaggag cgtgaccggg
     3721 ctgccataac tgaacgggaa gccgcgctgc cggagagtgt gctgcgtgaa tcacaacggg
     3781 agcgggaagc ggtccgggag gttgcccggg aaaatctgct gcaggagcgt ctgcagcaga
     3841 tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga gactgataca
     3901 ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga ttcactggct
     3961 gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca gcgggatatc
     4021 atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat attccagctg
     4081 aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc cctggtgtgg
     4141 gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg gctgtgggga
     4201 tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccctggta tgaggggaat
     4261 atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac gcgcagtggg
     4321 tggcgtacag cagcggccat tcttctgatg gccctgttgg gggcctttgt ccggcaccag
     4381 ttacggcatt gccgggctgc tgatgctggc agtcagccac cgactgtacc gggcggaaga
     4441 cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg cgcttaacct
     4501 tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga ccgttggtct
     4561 ggtgtcgtgt gcaggaaaat cattaccccg tttctggccc ggggattttt tcccggtatt
     4621 ctacgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg
     4681 accgtaacag gatgattcgt cagaggcaga acacagcatg acagagcaga agcgaccggt
     4741 actgacactg aagcggaaaa cggaagggga gacgcctgtc cgcagccgga aaaccatcat
     4801 caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg agaaagcagc
     4861 ccgggaagca gagctggcgg caaaaaaagc gcaggccaga caggcactgt ccatttatct
     4921 gaacctgccc acgctggatg aggccgtgaa taccctgaaa ccctggtggc cgggattatt
     4981 tgacggtgac accccccggc ttctggcctg cggtatccgg gacgtgttac tggaagacgt
     5041 ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga aggccatcac
     5101 ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg
     5161 gtatgtgacg gagcatattt ctcaggagga agaagcgtat gcggcagcgc gtctggataa
     5221 aatccgccgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg agaaataaaa
     5281 aaaagcctcc atcagagagg aggcagggaa ataaggctta aaaggaaata acttccgctt
     5341 ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac taaaggggga
     5401 caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc gttaccagcg
     5461 tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag cggcagaagc
     5521 atggcgacgg gctgcctgtg ttgcgcccag gacagactgg cagcagtttg cccgaaaaag
     5581 ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc agccttcatt
     5641 actccaggag tcagaaggct gaaaggagtt tatgagattt gcttttttta atatccggtc
     5701 gggcagtatg accggacagt tctgagtagc acagagtttt ttccgtatta agtcgtgatt
     5761 ttatattttg tgattaattt cacaaaataa ggtgttattc agtgtgtgct gcaatattca
     5821 ggatgcctga aatacagatg ctatttatct gatatggaga ataa
//
LOCUS       DAJVYM010000189         5806 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVYM010000189 DAJVYM010000000
VERSION     DAJVYM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.191"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(84..755)
                     /locus_tag="OQS60_003608"
     CDS             complement(84..755)
                     /locus_tag="OQS60_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS2389267.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(862..1095)
                     /gene="yedF"
                     /locus_tag="OQS60_003609"
     CDS             complement(862..1095)
                     /gene="yedF"
                     /locus_tag="OQS60_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS2389268.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1092..2297)
                     /gene="yedE"
                     /locus_tag="OQS60_003610"
     CDS             complement(1092..2297)
                     /gene="yedE"
                     /locus_tag="OQS60_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS2389269.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2484..2897
                     /locus_tag="OQS60_003611"
     CDS             2484..2897
                     /locus_tag="OQS60_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2389270.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(2931..4418)
                     /gene="amyA"
                     /locus_tag="OQS60_003612"
     CDS             complement(2931..4418)
                     /gene="amyA"
                     /locus_tag="OQS60_003612"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS2389271.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4496..4861)
                     /gene="fliT"
                     /locus_tag="OQS60_003613"
     CDS             complement(4496..4861)
                     /gene="fliT"
                     /locus_tag="OQS60_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS2389272.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(4861..5271)
                     /gene="fliS"
                     /locus_tag="OQS60_003614"
     CDS             complement(4861..5271)
                     /gene="fliS"
                     /locus_tag="OQS60_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS2389273.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5286..>5806)
                     /gene="fliD"
                     /locus_tag="OQS60_003615"
     CDS             complement(5286..>5806)
                     /gene="fliD"
                     /locus_tag="OQS60_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS2389274.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1425 a         1531 c         1428 g         1422 t
ORIGIN      
        1 cgttgaagca ggaacccacc gaaatgattc aggcgacagc ctgaacgtag cagggatcct
       61 cgtccttcag ggcgtggagg atgtcaacaa acaggttgaa ttaactccgc gccctggttt
      121 ttgacattac ctaccgcgcg cgataccggg tgccaggtga actggtttgc tggcacacag
      181 ccgttggtag caatttctga tgcttcttta ccgccaatct cttgccgcat ccattcctgt
      241 gcagcttctg gcgacagaac cagcgggcgg cggtcatgaa tgtctaccag accctggtct
      301 gctgcagcag tgacaattaa aaatccttcg gcttcgtcac cacgctcaaa tggtgtgcta
      361 cctatcgcgg ccataaaaat aggttgtcca tcagcacgat agataaaaaa aggctgtttt
      421 ttgtcgcctt cttttttcca ctcaaaccag ccatcggcaa aacagattgc ccgaccatgt
      481 tgccagagcg gtttaaacat gcgactggtt gcggcagttt ctacgcgggc gttaatcagc
      541 ggcggtttat cccaccatcc gggagcatat ccccagaaaa ccggatccag atgaaggtgt
      601 tcatcacgtt cactgagcag aagaactttg gttcccggcg cgacgttgta tctgccaatg
      661 ggttcgggat cgtagggaat atcgcgttca atatcttccg cgagaagcgc aaggtaatct
      721 tcacgcgttt gggattgggc aaagcgtcca cacatagaaa cctcctgaga tcatactgaa
      781 aagaatagga gagggggcgg gtaaaatcta tgtgtcagag gcaacaatgt tcccgctgtg
      841 ggccacagcg ggaatgaggg attacttctg aattaaataa cgaatggtcg gcccgtcttg
      901 ctgaatatcc agcaccgtat agccgtgatt acgcgcatcc agtggaatat tattgatcga
      961 ctgtggacag tcgctcacca cttccaggat ttcccctttt tttaactgcg gcatcgcctc
     1021 aagggttgcg actgccggat aagggcaggg ttcacccacc atatcaaggc ggtaatcagg
     1081 aacgatattt ttcatgcgat ctccttagca gtctgcggtg ccgcacggcg gaagaagcgt
     1141 ttttcccagc cgataatcaa catcagcgca gtaaacaaca gcaaatatgt caccagcagg
     1201 ccacccatcg ggccaaaggt tttcagcagg ttgattttgt cccagtcggt ggccagcgcc
     1261 ggagcgaaat catcccagta atacgccaga atcgtcgagc cgatcacatt gcccagaccg
     1321 acccaccagt agtgcacctg gccttctacc gcgcggtaca tccagccggt ttcgcagccg
     1381 cctgccagca cgatgccaaa accaaacagt aaaccaccaa ttaccgcgtt tggtcccgcc
     1441 cacatgattt tgggttcaac gcctaactgt acgtaactga agatcccgat ggcactcacc
     1501 gccataccga taatgattgc tttcgccata tgggtacgtc cggtgatcca catatcgcgg
     1561 aacgccgaag taaagcagat ttgcgcacgt tcaatcagta aaccaaagcc gacgccaaac
     1621 agcattgcca gccccagttt gggttggttc atcgccgtga gcagcgccca gcccagcatg
     1681 ccgaaaaaga ccagcatccc gagacgaaaa cgacgccgcg cctgatccgg tttttgcgtc
     1741 agcggtgagg cggcagaaac tttctgcatt ttcacgggaa tacggaagat gggcagcagg
     1801 gtaaagcgcg cgccaaacca tgaaccaatg gcagtggcga tggcaaagaa ccaggcatgc
     1861 agcgagaact gaggaatgcc ggtaaagaac gccgccaggt tacagcccat tgccagacgc
     1921 gcgccaaaac cggcgataat gccgccaata atggcctgca taatgcggat acggctgcgc
     1981 ggcatgcgca gtttgacatt gttggcccac agcgctgcgg caaagcagcc gccaaacatg
     2041 ccgaggatca tcatcccgtc gatgcgggtt aatggcgatc cttccagatg gataatttta
     2101 aaataacccc actcttcagc atggacgcca aacagctgca ggagctgacc gccccaacgg
     2161 gtaaattcac ccgtgacagc ccaaaaggtg ccagtaatgc caaaatagta agtggagaga
     2221 atacccgccg cgatgaccgc agggatgggt gcccagaatt taatcaacca ggcgtgtttg
     2281 aattgctgcc atgacatgaa tgtagcctct gaactcagaa ggtttgaaac aagaatcgcg
     2341 aatcatacat catgcaaatt tattttcggg gtaataattc acaaaaataa tagttagatc
     2401 aaatttctct ctgaacctgg aaaggctggc gacatcaggg ctttaatgcc acaatcctct
     2461 tttcttctca tgcaggacga atgatgaaaa aactcgcaat tgcaggtgcg ctcatgttgc
     2521 tggcgggctg tgccgaagtc gaaaactata acaatgtcgt gaaaaccccc gcgccggact
     2581 ggctggcggg ctactggcag accaaagggc cgcaacgtgc gctggtcagt ccggaagaga
     2641 taggcagcct gattgtcact aaagagggcg ataccctgga ttgccgtcag tggcagcggg
     2701 tgattgccgt gccgggcaag ttgacattga tgtctgatga tctcactaac gttaccgtaa
     2761 aacgtgagct gtacgaggtt gagcgtgacg gtaacaccat tgagtacgat ggtatgacga
     2821 tggagcgtgt ggatcgcccg acggcagagt gtgccgccgc gctggataaa gcgccgttac
     2881 cgacgccact gccgtaatga aatctccccg gcttgccgcc ggggatgaat ttaaatcacc
     2941 tcttcgataa cccacacgct gacgctgccg ccgttgcaaa agaaggtcgc ttcgccgttt
     3001 tcgtcggtca ctacgctctc ttgccgattc cccagaaaat cacgccaggt tttattgccg
     3061 taattctctc ccagatgaat ggttttttcg ccatcatccc cgttcgacat gaccaccacg
     3121 cagccgggat attcgtcagt gccactgcgg ctaaaggcaa tgcagttcgg atggtcgaaa
     3181 aataacgtct gtacaccgtg ggcgaaacgc tgacgggcga gaattaactc atcaagctgt
     3241 tcgattattg gcatatctat cggataggtt tgcccgtcac caccgacatc ttcgtaatgc
     3301 gcaccgtaga ggtccggata gaacaccgaa ggaacgccat tttcccgcaa caaaattaga
     3361 gcatacgcca gcggtttaaa ccacggttcg accggcgctt cgagggcttg caacggttgg
     3421 gtgtcgtgat tggctacgag ggttacggcg tggaaaggat cggcttccac taatgtaccc
     3481 gtgaaaatct gcgtcatgtc gtagtcgcgt cccatgcgcg atgcttcatg gaatttcatc
     3541 tgcagcggcg catcgaacag catggttttg ccttccacct gatcaatata cgtttgcagt
     3601 ttatcgactt catgcgacca gtactctgcc acaataaaca gcggctttgg cgcaacttcc
     3661 tgtacgtgtt cgatccactc tttataaaac cacgccggaa tatgtttaac cgcatcaaga
     3721 cgaaaaccgt cgcattgtgt ttgttccatc acccagcgcg cccagtattt aatctcttcc
     3781 gtcacggcat gattgcggaa atcgatattc tcacccatca gataatcgaa attaccgagt
     3841 tcatcatcaa cctgatcgtt ccagccttcg ccggtgtagt cgttaacaat tttaaatatg
     3901 ccatcttcat caggattttc gatatggtcg ataccgctaa aacatttgaa gtcccagata
     3961 aactgcgagt attgcccggc acgggcgggg aaggtgtaac gcgtccagcc ttcgcactca
     4021 atgatttctt catcaatttg cgtacggtca tcagcattta cacgctgcac gcgaatagct
     4081 tctttttcat ccgcccccat tttgtggttg actaccacgt ccagtagcac cgcaatgtca
     4141 ttacgtttca gagcgtcaat ggcggccagc agttgtgctt tatcgccata tttagtaggg
     4201 atgctgcctt tctgatcaaa ctcacctaaa tcaaataaat cataggagtc gtagccgacc
     4261 gaatacccgc ccgatgcgcc tttataggcg ggcggcaacc agaccatatt gataccaata
     4321 tcattaaaac cgtcggcgcg ctcggccagt tcaggccaga gcttaccgcc ttccgggtaa
     4381 taccagtgaa aacattgtaa cagcgtggga ttacgcattc ttcgactcca ttcaagggga
     4441 acattagaag cgtagccgta atcggattat tcgcgagcca tcggctcgtt aagattcaaa
     4501 agaggttatc ctgcggagcc agcacaaagc cgccctgatc tccataggca cttaacactg
     4561 atttttgcac cgatgactga ccgaccagtt tcgccagttc atccatacga atctgtaata
     4621 actgctttac cttgctttcg ttgtcgagaa tcaggcgcag catcgggcgg agctgctcct
     4681 gcatcgtggt ggacggggca acctcttcag tcagatgtgc aatctcctgc accgcattca
     4741 catacgccat ttcgctggcg atgagttcgt cccattgttc ttccgttgcc aggcgtaaca
     4801 tgagctggct tttatcgacg agttgttgcc aggcgaaata caaatgcggt gcatggttca
     4861 tcagactggg tcctgaatca aagaagggga gagtaaagac tctttccagg catcggcaat
     4921 attgcgcatt aatgcttcca cttcttcgac tgcggagaca tcgttgcgta aattggcttg
     4981 cagcaagcgc ctgaccatat agctataaag agcaatcaag ttttgggtta gttcgtcttt
     5041 gctctcttca tcaagactca cccgcagccc gttctcaatg atgttgatcg cttttgacaa
     5101 agagacgcct ttgccttgct gattgttgtc ctgcataaac aggcgcgctc taaccagtgc
     5161 gctcagcact ccatcaaata gcatggtgac cagctgttgc tggctggcgc tcattacggc
     5221 gctttcgacg ccaatttgtg cataggcttt tgagccgctc gcggtataca tgctgacctc
     5281 cgtgatcagg aattactcat tgcagtaaat tgttggctga gataggtact ggtgttattc
     5341 agtttgctca tcatggtatc taattgcgta aactgtgcag tgtaacgcgc cacggtgtca
     5401 tcaatgctgg cactgacgga tagatactgt ttggtcagtt ttttcagtgt ggcgttaata
     5461 ctgtcctgtg cgctatcaat aatgccatca tcggcaaggt aaccttttac ctcggtggca
     5521 attttggtgg taattcctgt ttctttacca tcgcctacca gcaattcacg tacggatgct
     5581 gtattttcat tcaggacttt tttgagttta tcatcatcaa ttttcagctt acccgtcgtg
     5641 ccgtcctgct taatcccgat ctcgtttaat gttttgaagg cactgtcgct ggcaccgttt
     5701 gcaaattgtg cgcggatgcc gctttgaatt gtacgtacaa cgctgtctcc gagaagtgca
     5761 ccattatttt tatcttgtgc ttcagcacct gggtctactt ctttat
//
LOCUS       DAJVYM010000190         5771 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVYM010000190 DAJVYM010000000
VERSION     DAJVYM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.192"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1416)
                     /locus_tag="OQS60_003616"
     CDS             complement(<1..1416)
                     /locus_tag="OQS60_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS2389275.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNL"
     gene            complement(1557..2897)
                     /gene="gntU"
                     /locus_tag="OQS60_003617"
     CDS             complement(1557..2897)
                     /gene="gntU"
                     /locus_tag="OQS60_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS2389276.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(2901..3428)
                     /gene="gntK"
                     /locus_tag="OQS60_003618"
     CDS             complement(2901..3428)
                     /gene="gntK"
                     /locus_tag="OQS60_003618"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS2389277.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3567..4562)
                     /gene="gntR"
                     /locus_tag="OQS60_003619"
     CDS             complement(3567..4562)
                     /gene="gntR"
                     /locus_tag="OQS60_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS2389278.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(4786..5481)
                     /gene="yhhW"
                     /locus_tag="OQS60_003620"
     CDS             complement(4786..5481)
                     /gene="yhhW"
                     /locus_tag="OQS60_003620"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS2389279.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5604..>5771)
                     /locus_tag="OQS60_003621"
     CDS             complement(5604..>5771)
                     /locus_tag="OQS60_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2389280.1"
                     /translation="MKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
BASE COUNT         1474 a         1584 c         1383 g         1330 t
ORIGIN      
        1 gagatttaat ggcaggtcag cgtgtttaat aaataaattt gccgcatgga gtaagccgct
       61 atcaatatcg ggcgacaaca acggatgctg aagacaactg ctacatttac cacaacgttc
      121 ggcacttggc tcatcgagtg cgcggcgcag aaattgcatt ttgcactcct tggtcgtgat
      181 atagctttca acatctgcca gctcattttt tctctgattc atcaaatggt taattcgttc
      241 atggtcagga gagaaactga cgatagttct gcgccataat ttctcggtat acaccacagg
      301 cgatggattt tcagctacca ataatttcag tgctttttct atttgcccat aacggagatt
      361 cgtccgttgt tcaatgcctc gtagggtaag accgtcattc tcgctaagta cgttgagtat
      421 ttcatgaatt tgcgcctccg caggaaaggc gctttcacgg aagaatttat gaatagcgcg
      481 gtcttcacca ccacaaagca atatgccaac tgcggaatct atagcacgcc cggcacgccc
      541 tacctgttgg tagtaaccga caatagaccc cggcatttga taatgaatga cgaaacctaa
      601 atcaggttta tcaaacccca tacctaatgc cgtcgtcgcg accagcactt tgattttatt
      661 tgccagcagc gcctgctcca gatattccct ggcagtatta gaatcttccg cgccttcaca
      721 agtcacgccg ctgtagtaag caaatgcact gataccattt gtccttaacc actcggcgac
      781 aagttcggca tcgcgagtag tcagggtata aacaattccc gatttggaaa actgagggat
      841 aaccgttgct aaccatgcca gacgggatga ctgttcacct aagactaagg catcaagcgc
      901 caaactttcg cgagccagcg ttccacgctg aatcacaatg tcacccaatt gctgacggat
      961 atcctcaacg acacgattat tcgctgtcgc ggttgtaccc agaacagggg tattcgcagt
     1021 ttcaagaaaa tcctgattcg ccaaccgttc aggtgaaatc agcagacaat ctacaccacc
     1081 ccgcagcaac ttatcgctaa tgcgctgcca ttcttccctg tttgtagaat tcaacgtttc
     1141 agccgtaata cctaaccgtt cagcggcggc aacctggtta cgcatcaggg caagcaaagg
     1201 agagatgata attgtcgggc cagcgccgcg atcgcggaag attttactgg cgatgaagta
     1261 cacggcactt ttcccccagc cagtgcgttg gacaaccagc agttttcgcc gctgattaac
     1321 caccgcatct atagcttccc attgcccttc cctgaaagta gcgctggtgt tacttaacat
     1381 ccgctgtaac agcaattcag ctccgtgttt ctccatcatc ttctccctcg tccgttaacg
     1441 cagtgattag aacggataat gttccggtct ttttgcgaga gagatcaaaa agatgaagac
     1501 aggagataag agaaatgaat tactgcgagc aagctcgcag taatttgtcg cagtaattaa
     1561 ctcaacagct ggaacgcaat catcccaaca atggcaccaa cagtgccgag gatggtttcc
     1621 atcatggtcc aggttttcag cgtttcggct tcggtcgcgc cggtaaattt accgaacaac
     1681 cagaaaccag cgtcgttaac gtggctgaca acaatcgaac caccggcgat acaaatcgac
     1741 agcgccgcca tttgcgcacc ggaattgttc agttgttcaa tgaccggcat caccagcccc
     1801 accgccgtta aacaggcaac ggtggcagac ccctgaatga tgcgcactgc agctgccagc
     1861 acgaagcagg tgatagcaat cggcaggccc atgccggtta acgcttcgcc cagtgccgga
     1921 cctacgccag agtcaaccag cacctgtttg aacacgccgc ctgcaccaat caccagcaga
     1981 atgatccccg ccggttgcag cgcgtgaccg caaatctcca tcactttgtc ttttggcatg
     2041 ccctgacgca ttgccaggcc ataaatcgct accagacaag caaccagaat cgcggtaaac
     2101 ggatgaccaa taaactcgaa ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg
     2161 gcaatggttt tcagccccac cagcaccagc ggcaacagga tcagcgacag gctgaatccg
     2221 aaagatggca ttttaccttc gccgagatgc ggctcgttga tgtcgtcagg aatatgcagc
     2281 tcaacgtaac ggctgatgaa gttaccccac agcggcccgg caataatcat tcccggaatt
     2341 gccgcacaca ggccaatcag gatcatccag ccaaaatcgg cgttcatctg cgatgccagc
     2401 agcattggcg ctggccctgg caccaggaac gccgccgctg ccgccacgcc tgcgaataat
     2461 gggattatca gcttcaccag gttcgtatcg gtgtggcgcg ccattgagaa agcaacgcta
     2521 atcagcagaa caatcgccac ttcaaagaac agcggtagcg cacagaccag ccccgcaagg
     2581 ccgatggcat aatgcgcgcg gctgtgaccg aaggatttga gcattttgac ggcaatctga
     2641 tcgactgcgc cggtttcatg taagatcttg ccaaacatag ctcccagggc gacaaccacc
     2701 gccaggaagc cgagggtgcc tcccatccct ttttccatcg tcgctgcgat tttatcgagc
     2761 ggcataccgg aaaaaaggcc agcccccatg gacaccacca tcaaagccag gaaagcgtgc
     2821 atacgcgcct tcatgactaa aaacagcagc agtaaaacag accctactgc tgttaaaaca
     2881 agcgttaatg tagtcactac ttatttgcct tttttaataa cctcaatggt gcttgccaca
     2941 acaccttcca gcggttgatc gatatccacc accagtacat cggtttcgtc cgcacccggc
     3001 tcctgcagcg tttcaaactg cgtcaccaac atttgggttt taaagaaatg gcctttgcgc
     3061 gctttcaggc ggctttcaat cacgtcaaaa tcgcctttca ggtagatgaa agagagattc
     3121 ggattacctt cacgcagcaa gtcgcgatag tgttttttca atgcagaaca gacgatcagt
     3181 gacactttat tagtgcgctg catagcaaac gcggcgtcgt tcagcgcctg caaccagggt
     3241 ttgcgatcgt cgtcattcag cggttcgcca gacgccattt tttcgatatt gcgccgtgga
     3301 tggaggaaat cgccatcaag aaacgcggca tgaagttgat gcgccacttc actggcgacc
     3361 gcagatttgc cgctgcccga tacgcccatc aagacgtaaa tgtggtgatc atggttagtc
     3421 gtgctcaaag tggtgccccc acaatacaag aattaaaaat gttacgggta actgttatcg
     3481 gtaacattgt ccagccggac aatatcagaa gcaatatcca tccgtgcctt aagtgtataa
     3541 gtgtgagcta cttcaaattt gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct
     3601 aaatctaaca ttttcggtgt cacagattcg ccacgaatac gcgccagcag gcgttcagcg
     3661 ccaatactgc ccatccgctc acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc
     3721 tggccaatgt catgaccgtg gaaaccggca atcgccatat cgtcaggaac cttcaatccc
     3781 agacgctgac attcaaacgc cgcgccgacc gccaggtcat cgttggtaca gaacacgcca
     3841 tccagctgcg gatattcccg ccgcgcctgg cgaatcagtt caataccgga agagtaagaa
     3901 gaagattgct caaccatcac gctatacggc accagacctg catccagcat cgcctgttcg
     3961 tatcccttct gtttgatgat agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg
     4021 caatgcccgc gagcaataat ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa
     4081 ccgacggcga tatcgaggca cggcgactgg ctgtccatga gttccaccac cgggatcccc
     4141 gccacttcaa tcatctttaa ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg
     4201 tcgatattcc aggagagcat ggattcgaga cgttcttgct ccatttccgg tttataaccg
     4261 tagtgagcca gcatggtctg ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat
     4321 acttccgcga aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg
     4381 gcgttagaga ggatatcggg cgcacgattg ggaatatagc ccagttcatc aagagcggcg
     4441 gcaatcttgc cgcgtagagc gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc
     4501 gtcattttgg tcacgcctac acggtcagcc acatcctgaa gtacgggtct tttctttttc
     4561 atcgtcctga aggtacaaaa gagatagatt ctcttagttt aacacggacg catcagaacc
     4621 ttcccgggga aaacagtgct tgtttacaat tattagatat acactaaatc aattgcatcg
     4681 cattgtgcta acgacgcggt aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag
     4741 cgaggaagcc tgcaaaaagg caggcttcaa agatgccgtt gagttttaaa ccggcggcag
     4801 atcgaacagt aacacttcgc tgtcgctatc cgcatggatg gagattgcct gctcatccca
     4861 gattgccaga ccatcgctgg tcgaggcttt cacgccgtta atggtgacat tgcctttcac
     4921 cacctggatc cagacgcggc gttcagcggc aatctgatgc accgactgct catctttcag
     4981 caacgcccag cggtacagtt ccatatcctg atgcactttc aatgaaccat ctcgcgcatc
     5041 cggcgagagc accagctgtt tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg
     5101 cgtaataccg ttttcttcag gcatgatcca gatctgatac agatgcagac gctcggtgct
     5161 gcttgggttg tactctgagt gacgaatacc cgtacccgca ctcataatct ggaactcacc
     5221 cgccggaacc tgctctttat tgcccatgct gtcctgatgc tcaacagtac cttccagcac
     5281 gtaggtcaaa atttccatat ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat
     5341 cacgtcgtcg ttaatcacgc gcagcgcgga gaagcccata aagttcggat cgtaatagtt
     5401 ggcaaaagag aaagtatgcc aggagtccag ccagccatga tttgcatgac cacgttcatt
     5461 tgctttgcgt aagtagatca tttgtattcg ccccatgaat gatttcgatg ggcttagtgt
     5521 ggacccgatc tgcctgggat gatagagggt gaaaattgac ccctctgttc aaaaaaattg
     5581 aactattcga ggggccatca aacttacttc gcgagagtca ctgtggaggg agaggcttgc
     5641 tcaaatccgc gttcaaggat ttccagattg gtaagaactt cagattcctt gacgtaattt
     5701 ggcgcaccgt gggtgatggt ttgatacaac gcatcataaa cgcgcccgta atcgcccatc
     5761 tccggcttca t
//
LOCUS       DAJVYM010000191         5768 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVYM010000191 DAJVYM010000000
VERSION     DAJVYM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.193"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..321
                     /locus_tag="OQS60_003622"
     CDS             <1..321
                     /locus_tag="OQS60_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2389281.1"
                     /translation="TIIHTDLSAPWRLKDISSRLYLSESLLKMKLKEEGVSFSKIILD
                     ERMQMAEYLLSTRCYPISKVAKVCGYASVSYFTYVFRRYFGVSPSQYSQRSSESKILT
                     HQGI"
     gene            complement(363..815)
                     /locus_tag="OQS60_003623"
                     /pseudo
     CDS             complement(363..815)
                     /locus_tag="OQS60_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            879..1025
                     /locus_tag="OQS60_003624"
                     /pseudo
     CDS             879..1025
                     /locus_tag="OQS60_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1025..2182)
                     /gene="intS"
                     /locus_tag="OQS60_003625"
     CDS             complement(1025..2182)
                     /gene="intS"
                     /locus_tag="OQS60_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS2389282.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2344..2418)
                     /locus_tag="OQS60_003626"
     tRNA            complement(2344..2418)
                     /locus_tag="OQS60_003626"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2383..2385),aa:Arg,seq:cct)
     gene            complement(2494..3426)
                     /locus_tag="OQS60_003627"
     CDS             complement(2494..3426)
                     /locus_tag="OQS60_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS2389283.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3720..4475
                     /gene="mlaA"
                     /locus_tag="OQS60_003628"
     CDS             3720..4475
                     /gene="mlaA"
                     /locus_tag="OQS60_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS2389284.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4657..4992)
                     /locus_tag="OQS60_003629"
     CDS             complement(4657..4992)
                     /locus_tag="OQS60_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389285.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(4950..5342)
                     /locus_tag="OQS60_003630"
     CDS             complement(4950..5342)
                     /locus_tag="OQS60_003630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389286.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5349..5725)
                     /locus_tag="OQS60_003631"
                     /pseudo
     CDS             complement(5349..5725)
                     /locus_tag="OQS60_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1426 a         1314 c         1279 g         1749 t
ORIGIN      
        1 acgattattc atacggattt atctgcacca tggcgtctta aggatatatc atcaagatta
       61 tatctcagcg aaagtttact aaagatgaaa ttgaaagaag agggggtatc attcagtaag
      121 atcatacttg atgagaggat gcaaatggct gaatatttac tcagcactcg ttgttatcct
      181 attagtaaag tagctaaggt ctgtggttat gccagtgtct catactttac ttatgtattt
      241 agacgttatt ttggtgtttc tccaagtcaa tactctcaga ggagttcaga aagtaaaatt
      301 cttactcacc agggaatctg atcattgttc ttgccccctt atttccagac agggggtgta
      361 tcttaagtta acgttacccg ctgacgtcga tattctcgcg gagagcgata acccagcgca
      421 ctgtgcggat ggttttcatt gtaatgttcg atcgccactg caagattatg caatgccgtt
      481 cttacattcg gtttcggcat gaacgcgatg tagtcttcct tcatcgtttt cacgaacctt
      541 tctgccattc cattactctg aggactgctg attgccgttg tgcagggctc cagattcaac
      601 tctctggcga actgccgtgt ttcatgcgct ctatacgctg aaccgttatc tgtcagccac
      661 tggatggact gttccggtac tttatcgcca aagcgttttt ctatcgctcc taacatgaca
      721 tcctgcactg tcgctttatc gtaacctccc gtgcttgcgg cccagtctat ggcttcacga
      781 tcgcaacagt ccagggcgaa cgtaacacgc agttttgaat cgccacgggt ttaacagaca
      841 cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag cgttatcccg
      901 aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct gttgccagcg
      961 ttgcaacacg tctcgatatc actacccaca gcctttatgc ctggataaag aagtacggtc
     1021 cggattactc cactttttca tcaagccaat ccgcccacca ctgcatcatt tctctgcgct
     1081 tatcgagata ctgagcatgg ttgtaaatac cgcgcacaga tccgccgttg gcatgtgcca
     1141 gctgcacttc aatagcgtca gcaggccatt cgtgctcgtt cataatcgtg ctgaattcat
     1201 gcctgaatcc gtgaccgctt tccagacctt catagccgat ttgtttgatc acaagcaata
     1261 ccgcgttctc gcagattggc ttcttcttat cgttgcgccc ggcaaaaaca aactctgaga
     1321 ctggtttggt gattgagctt agcgtagtga gaagttcaac cacctggtct gacatcggga
     1381 ccacatgaat tttgcgtccc ttcatcacac tggcgtcgat ggtgataatc ctgttttcaa
     1441 aatcgacgtt cttccatagc atggaacgaa gctcttttgt tcttagggct gtgtagcgta
     1501 aaactttggt cgcaatgagc gatacgatac ttcctgaaaa tgttgccagt gctttgttga
     1561 atgcaggcat ctggtctgct ggaaggaacg ggaagttctt cttgcggtat cctttcatgg
     1621 cgtctgcaag gtcaggtgcc gggttatatt tagcccttcc ggtgacaata gcgtaacgaa
     1681 aaacctcgcc gcatcttctg cgggctttgt tggctcgctc cattgcaccg cgatcttcaa
     1741 atctgcggat tacttccagc agttgcatcg gctcaatatc ctgaatctca agaccgccga
     1801 tgatgggtaa aatgtcgtca tcaaacattt tggcaagttc agttgcatag cctactgacc
     1861 agacttgctt cttgtgctcg taccattcct tgtaaatcgc actaaatgaa ttgttgttag
     1921 acgaagcctt tttcgccttt accggaacga tgccaaccga gatgtctttc ctcgcagtcc
     1981 atgccttatc ccttgcttcc tgcaaagtca taagcggata ttttccgacg gtcaggattt
     2041 tctccttacc gtcaatcttg tagcgaagct gccatacctt tttcccggat acaggaacat
     2101 aaaggtacag gccattacca tcgagaaggc ggtatggttt ttctttcggc tttgctgctt
     2161 caatctgctt aacggtgagc atgggtaaaa atccggtggg taaaattatt ttatccactt
     2221 tttacccgtc atggagtgcg gctgtcaacg atctgacgcg aaccatgacg aaccgtgaat
     2281 atacggaagg cttgatattc aggggatttt gcggactggt acggatggga gcgaactgat
     2341 aaatggtgtc ccctgcagga atcgaacctg caattagccc ttaggagggg ctcgttatat
     2401 ccatttaact aagaggacaa tgcggcatga gtatacccgc taatggagtg cggggtaagt
     2461 acgctgccgc tcgattgctt aaaccctcgc catttatgcc gggtttttat catttttctt
     2521 aatgttttcc gcacgttctg ctttttggcg tgcttctgct ttacgcttgt tgctcatgtc
     2581 gttacgaatc tgtgcatgac tcattaacgc gaagataaag gtgccgccgc agatgttccc
     2641 cgctaaagta ggtagtgcga agggccagat gaaatcgctc cagtgcagcg taccgttaaa
     2701 caccagatag aggatttcaa cagaaccgac cacgatatgg gtggtgtcac ctagggcaat
     2761 aagccaggtc atcaatataa tcaccacaat ctttgccgca cctgctgcag gaaacatcca
     2821 taccatagtg gcgatcagcc agccggaaat gatcgcgttg gcaaacatct cgctgggggt
     2881 gttcttcatc acatccatgc cgattttgac aaatgcatcg cgagtttctt cattgaagat
     2941 aggcatatat tcaaatgccc acgcagcaat accagtcccg agaatattac ccagcagcac
     3001 gactccccat aaccgcataa gtaagccaac gttgctcatt gtcggttttt gcatgacggg
     3061 tagtaccgca gtcacggtgt tttcggtaaa taattgctgg cgggccatta tgacgataat
     3121 aaaaccaaag gtataaccga gattctccag taagaagctg cccggcacac cttccagttc
     3181 gacatgaaat atcccttttg ccagtaacga agcgcccatc gacagacccg ccgcaatggc
     3241 tgaccacagt agcgccattg cgtcgcgttc cagctctttt tcaccatcct ggcggatatg
     3301 ctcatgaatt gccatcgccc gggaggggag tcggtcttca tctatttcta tttttttgcc
     3361 gcgctctttt tcttcgctct caacttcaat ttcgtcgctg tgttgatcaa ttttgtcgtt
     3421 gtccatggtc tcttctcttg aattagcacg tatagctaaa gcgtagcggc ttttttgctc
     3481 gcaactggcg ggagttactc tgaaaatgta gaaaaggctg cgtttgcctt tttctgtttc
     3541 tatagaatca agtagcctac agggcggcga ttaccaggct atgatcaaat cagcaaatca
     3601 gggcgtctgg acatcagttg acgtgctgtt acaatcgccc acacctaaac aggcggatac
     3661 ggtatcgttc cgtcatggat ggcaaactgc ataagccata aaaaaacagg gagacattta
     3721 tgaagcttcg cctgtcggcg cttgctctgg gaactacgct tctggtgggg tgtgcgagtt
     3781 ccggtacaga tcagcaaggg cgttctgacc cgttagaagg gttcaaccgc accatgtaca
     3841 acttcaactt caacgtatta gacccgtata ttgttcgacc ggtcgctgtc gcctggcgtg
     3901 attatgttcc gcaaccggcg cgtaatggtt tgagcaactt tactggcaac cttgaagaac
     3961 ctgcggtgat ggttaactac ttcttgcagg gcgaccctta tcaggggatg gtccacttta
     4021 cccgcttttt cctgaacacc attttgggga tgggcggctt tattgatgtt gcagggatgg
     4081 cgaacccgaa actgcaacgg actgaacctc accgcttcgg tagtacgctt ggtcattatg
     4141 gcgtgggtta tgggccttac gttcagttac cgttctacgg tagcttcacg ctgcgtgatg
     4201 acggtggtga tatggcggat ggtctttacc cggttctttc ctggctgacc tggccgatgt
     4261 ctgtgggtaa atggacgctt gaagggatcg aaacccgcgc tcagctgctg gattccgatg
     4321 gtctgctgcg tcagtcgtcc gatccttata ttatggtgcg cgaagcgtac ttccagcgtc
     4381 atgatttcat cgctaatggc ggcgaactca aaccgcagga aaatccgaac gcacaagcga
     4441 ttcaggatga tttaaaagat attgattctg aataagaaac aaataaaaaa ggtgagtcgc
     4501 aaaactcacc ttttttgttg ttatccctca attaccacaa gtaaaaggag ataaaccgac
     4561 aatgacccgg acgtaatgat gtaaaaatac tgatgttcgt cactgactat ttccgatatt
     4621 caggcgttga aaaatcaaaa aactggaaaa ataattttaa atatcatcaa aattaacacc
     4681 tggcgcagat agttgattaa tttcattttg atatttcgcc tggcgatttt gaagtctttt
     4741 ttcaacgtta tcttcgagat ccttaaagtc tttgtattta ttttcaggat ggtcaacgtc
     4801 aactttgtta gcgaataaat tagaaattat ttcttttaca ttgtcgcgtc taaattttgc
     4861 atcgtccact tgaaaattat atcttattgc tcgacctaaa cgtttaaaaa cttcgttagg
     4921 ttctctcagt ctttccccgt tgagctcgtt tacaacctga tgatcggaac ctgttgcaaa
     4981 tgattctgcc atgatagctt tactgctttc aaaccacgct gaagcattgc tgaagcattc
     5041 ctggcttttt cactacaata ttgggtgaga ctatcaccat taatacgaat tcctctttga
     5101 atgtggtagt cgtcctgatc gtagtaaccg agagataagt aatcatttcc gtttttattt
     5161 ttaattacaa tagcgagaga gttgcctttt ttatctttac cgtaccaggt aaataagtcc
     5221 gttggttcgc tattcccgct ctcatcaaat tctgttaccg tatgtctgag tttgtgtgat
     5281 atcttatcga agataaaacc aggatctcca tcatatattt cttttaatgc gctgaagatc
     5341 atttccttgt tattgatatc atgagtcttc tcaactatgt tgtcattaat gatagtacgg
     5401 acgagttctt tgtctttata gttcgcaggt aattctattg gttcttggtt gtcgatacca
     5461 atagtaatat ggtctgtatc attggagcca atatcaatat aagtaactga ggaattatta
     5521 atattaaatc ttaaggatcg tcgtttactc gtatggcgtt ttttataagc tcaagtagat
     5581 gtttaataca ggtgcttatt ccttccttac cattaaaaag gtttttgagc tcttgtacta
     5641 atccattaac ttcatcattg ttattttcat taatggattc tgggtaagtt gcgctggtat
     5701 tgttgattga aatagatggt agcatgattt tattccattt tgattgattt gttacaaata
     5761 tatattac
//
LOCUS       DAJVYM010000192         5614 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVYM010000192 DAJVYM010000000
VERSION     DAJVYM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.194"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="OQS60_003632"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="OQS60_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS2389287.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="OQS60_003633"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="OQS60_003633"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS2389288.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="OQS60_003634"
     CDS             2475..4124
                     /locus_tag="OQS60_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS2389289.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="OQS60_003635"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="OQS60_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAJVYM010000193         5579 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVYM010000193 DAJVYM010000000
VERSION     DAJVYM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.195"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..378
                     /locus_tag="OQS60_003636"
     CDS             <1..378
                     /locus_tag="OQS60_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389290.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
     gene            complement(411..1931)
                     /locus_tag="OQS60_003637"
     CDS             complement(411..1931)
                     /locus_tag="OQS60_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389291.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2022..2741
                     /gene="tonB"
                     /locus_tag="OQS60_003638"
     CDS             2022..2741
                     /gene="tonB"
                     /locus_tag="OQS60_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS2389292.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(2781..3179)
                     /gene="yciA"
                     /locus_tag="OQS60_003639"
     CDS             complement(2781..3179)
                     /gene="yciA"
                     /locus_tag="OQS60_003639"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS2389293.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3284..3823)
                     /locus_tag="OQS60_003640"
     CDS             complement(3284..3823)
                     /locus_tag="OQS60_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS2389294.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3853..4596)
                     /locus_tag="OQS60_003641"
     CDS             complement(3853..4596)
                     /locus_tag="OQS60_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS2389295.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4624..4713)
                     /locus_tag="OQS60_003642"
                     /pseudo
     CDS             complement(4624..4713)
                     /locus_tag="OQS60_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4953..>5579
                     /gene="ompW"
                     /locus_tag="OQS60_003643"
     CDS             4953..>5579
                     /gene="ompW"
                     /locus_tag="OQS60_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS2389296.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTIANYKLGGAQQHDSVRLDPWVFMFSAG"
BASE COUNT         1522 a         1334 c         1317 g         1406 t
ORIGIN      
        1 gctaacggtc ttcgtattca gatgaccttc caaggtgtag agtttcgttc aatcgtaggt
       61 cagactgatg tttcggctgc tactggaaag accgctacca cgaatgatgg cggtgctacc
      121 agtaaaaaga acaccggaaa cactacaacg agccttggaa gccctatgct gacctgtaaa
      181 gagctttttg gctattcggc aagtgagtta tcagatgaag cgctaaaagc ccgtgtaacg
      241 tgctctaaga gcgttagcgt taaaaatggt gagagcacct tctcagatgc cgctaatgag
      301 caagcttcaa aggtgcttaa gccgggtaat gctctgcgaa agtactctgt gaaccctaac
      361 aagaagggca cttattgaga cataaaaagg ggcttcatag cccctgaaaa ttacagttga
      421 taagcgtcaa tatccagtag ctctttatca gcaatttcaa agattggtga cccatcattt
      481 agcggtaacg ggtttgtagg tatggtgatt tcagtgtcgt tctgtagcgt tacagttacc
      541 gaatccttac ccaatgtaag cccttttaga tgcttggaca aaatcaactg acactcgaca
      601 cgacccgctt tagtcataag gtcaatatct ttaagctggg gtagttcctt atcagtgagt
      661 gaagccttac gcctcatgtt atcaatcgtg gtctgtagtt ctgtgcgctt ctggttcagt
      721 tccttaagct gaatttttag ttcatcaata tcatccatct caagaagtag ggcggtagct
      781 ttcttgattc tggctgtgac aatttcaacc tcatgctgta acgctgcaat ctttctctct
      841 gattctccat cattccccac gtcaatcatc cccagaaaat tatcaatcat ccatcggtca
      901 aacggtttac gcttcaacgg tggaacatca cagcgaccgt ctaagtggtt attgcacctg
      961 tagaccccag catacttatt ctttgttccg gtaggatgga cactagcccc acactgacac
     1021 ctaaacagac ctttgaagat gttaatcgtt agtgggttgt cacttgcagg tgtacggcct
     1081 ttacgattct tgtcgagaat ctcttgagct ttattgaacg ttggtatatc tacaacacct
     1141 tcgtagtagt tcggtatagc ggggcggtca ttacgttgtg atgctggtaa cgtgcctatt
     1201 gttgtctctg accttagaat ggtgcgtaca gagtgaacag agaacccatt agcatcttta
     1261 cggcgactaa acgactttat accgttgctt tgaaggtgac gtgcaatgtt atgcagactt
     1321 tcaccatcta gcagcagttt gaatatcaat ctcacagtct cagcgtgttc attgagttta
     1381 aaagtctcag ggtcaatcca tccaggaacc atctttgtaa gacgttgttc accagcaagg
     1441 gcaagttttg cacgttgccc ccatttctga gaaacacgga aagacttctg cttagatttc
     1501 tgatgcgcta tgtaggcttt gacacgctgg atgatgctta tgccaaggtc atcggctaaa
     1561 gcatcctttg tgtagataag gccatcttgc aatgtgtgaa tctctatccc gttgcctaag
     1621 atgcggttca tcaaggcttc tgtttcaaga tgctctaatc ggcctatacg gtcgatactc
     1681 tcaactagca gaattgtacc ttctggaaac tccccagcct caatacgggc taacatgtca
     1741 ccaaactgac cagtaaggtg tttacctttg taaccactca acccttcatc actgaaagac
     1801 gctactattt gagcatcgcc attcttcttc agccactgct tgaacaggtc gttttgtcgt
     1861 ctggttgaat ctgcaccttc agcgccttgt atggctgatg agaatctcaa gtatgttatt
     1921 acggttctca cggtgataaa ctcccatgca attgattatc atttaaagat gaacttataa
     1981 acgttatggc taatgctaca atgccgaata gctacaatac aatgaccctt gatttacctc
     2041 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcgggtc
     2101 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcag ccaatttctg
     2161 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
     2221 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
     2281 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
     2341 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
     2401 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
     2461 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     2521 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     2581 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     2641 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     2701 atatcctgtt taaaattaac ggcaccaccg aaattcagta agtagaaagt caaaagcctc
     2761 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     2821 accgcgacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     2881 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     2941 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     3001 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     3061 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     3121 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     3181 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     3241 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     3301 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     3361 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     3421 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     3481 ccggttgcgg cagcgtgagt tctttaccca gcatccgctg aattagcggc tttttcatca
     3541 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     3601 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     3661 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     3721 tcgccgtggc gacgatcagc gccgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     3781 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     3841 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     3901 ggctgaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     3961 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     4021 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     4081 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     4141 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     4201 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     4261 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     4321 accgattaat cctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgttgttc
     4381 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     4441 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     4501 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     4561 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     4621 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     4681 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaacc
     4741 gcggcaagtg gcgaacgagt taatatttgc gtagcgaaaa tatctaaaaa ttgatttaaa
     4801 tcacgttaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaagggtgaa
     4861 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     4921 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     4981 cagtaacaac tcttctctct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     5041 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     5101 gtggattcag cgtgaccaat aacacgcaac tgggcctgac gtttacttat atggcgaccg
     5161 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     5221 cgaccggcga tattgcaacc gttcatcatc tgccgccaac actgatggcg cagtggtatt
     5281 ttggtgatgc cggcagcaaa ttccgtcctt atgttggggc aggtattaac tacaccacct
     5341 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     5401 aagattactg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     5461 tggttaacat gtcagtgtgg tacatggata tcgataccat cgccaattat aaactgggcg
     5521 gtgcacagca acatgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcagga
//
LOCUS       DAJVYM010000194         5442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVYM010000194 DAJVYM010000000
VERSION     DAJVYM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.196"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..855
                     /locus_tag="OQS60_003644"
     CDS             <1..855
                     /locus_tag="OQS60_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2389297.1"
                     /translation="FGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPG
                     TKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAET
                     IGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVG
                     GIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEH
                     LELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQ
                     DTPDMREY"
     gene            923..4465
                     /locus_tag="OQS60_003645"
                     /pseudo
     CDS             923..4465
                     /locus_tag="OQS60_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1331 a         1347 c         1726 g         1038 t
ORIGIN      
        1 tttggcaacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
       61 gacccggacc agccgattat tatggggcgt acctaccacc aggaaaaccg cacacccggc
      121 agcctgccgg ggacgaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      181 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
      241 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgct
      301 gaaaccattg gcaacaatca gatgattgcg gttaccaaca atcagataga gacggtgggc
      361 gttaaccaga tagagacggt gggcagtaac cagataatta aagtgggttc tgttcaggtg
      421 gaaacgattg gacttgttcg tgcgctgacg gtgggcgtgg cgtatcagac gacggtaggt
      481 ggcattatga acacctcggt ggcactgatg cagtcctcgc agatcggttt gcataaatcg
      541 ttgagggtcg ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt
      601 aaaacgaaaa aggatgatac cgggcagacc gcgatttact ccgccggtga gcatctggag
      661 ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gacaaatttt tctcaacggc
      721 acaaaaattc atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg
      781 aactgtgcag cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat
      841 atgagagagt actgatcccg tacctgttcg gtattcagta acaaaccagc attaaaaata
      901 aacagaagaa ggaatttgaa tcatgagcgg aaaaccagcg gcgcgtcagg gagatatgac
      961 tcagtatggc ggtcccattg tccagggttc ggcaggtgta agaattggcg cgcccaccgg
     1021 cgtggcgtgc tcggtgtgtc cgggcgggat gacttcgggc aacccggtaa atccgctgct
     1081 gggggcgaag gtgctgcccg gcgagacgga ccttgcgctg cccggcccgc tgccgttcat
     1141 tctctcccgc acctacagca gctaccggac gaagacgcct gcaccggtgg gcgttttcgg
     1201 ccccggctgg aaagcgcctt ctgatatccg cttacagcta cgtgatgacg gactgatact
     1261 caacgacaac ggcgggcgga gcattcactt tgagccgctg ctgccggggg aggcggtgta
     1321 cagccgcagt gagtcaatgt ggctggtgcg cggtggtaag gcagcacagc cggacggcca
     1381 tacgctggcg cggctgtggg gggcgctgcg ccggatatcc ggttaagccc gcatctttac
     1441 ctggcgacca acagcgcaca ggggccgtgg tggatactgg ggggggctga gcgggtgccg
     1501 ggtgctgagg acgtactgcc agcgccgctg ccgccgtacc gggtgcttac cgggatggcg
     1561 gacgctgacg taccggcgtg aggccgccgg tgacctggcc ggggaaatca ccgacgtgac
     1621 ggacggtgcc gggcgggagt tccgtctggt gctgaccacg caggcgcagc gtgcggaaga
     1681 ggcccgcacc tcttcgctat cttcttctga cagttcccgc cctctctcag cctcagcgtt
     1741 ccccgacaca ctgcccggta ccgaatacgg ccccgacagg ggtatccgcc tttcggcggt
     1801 gtggttgatg cacgacccgg catacccgga gagcctgccc gctgcgccac tggtgcggta
     1861 cacgtatacg gaagccggtg aactgctggc ggtatatgac cgcagcaata cgcaggtgcg
     1921 cgctttcacg tatgacgcgc agcacccggg ccggatggtg gcgcaccgtt acgcgggaag
     1981 gccggagatg cgctaccgct acgacgatac ggggcgggtg gtggagcaac tgaacccggc
     2041 agggttaagc taccgctatc tttatgagca ggaccgcatc accgtcaccg acagcctgaa
     2101 ccggcgtgag gtgctgcata cagaaggcgg ggccgggctg aaacgggtgg tgaaaaaaga
     2161 actggcggac ggcagcgtca cgcgcagcgg gtatgacgcg gcaggaaggc tcacggcgca
     2221 gacggacgcg gcgggacggc ggacagagta cggtctgaat gtggtgtccg gcgatatcac
     2281 ggacatcacc acaccggacg ggcgggagac gaaattttac tataacgacg ggaaccagct
     2341 gacggcggtg gtgtacccgg acgggctgga gagccgccgg gaatatgatg aaccgggcag
     2401 gctggtatcg gagacatcgc gcagcgggga gacagtacgc taccgctacg atgacgcgca
     2461 cagtgagtta ccggcgacga caacggatgc gacgggcagc acccggcaga tgacgtggag
     2521 ccgctacggg cagttgctgg cgttcactga ctgctcgggc taccagaccc gctatgaata
     2581 cgaccgcttc ggccagatga cggcggttca ccgcgaggaa ggtatcagcc tttaccgcca
     2641 ctatgacaac cgtggccggt taacctcggt gaaagacgca cagggccgtg aaacgcagta
     2701 tgagtacaac gccgcaggcg acctgactgc cgttatcacc ccggacggca accggagcga
     2761 gacacagtac gatgcgtggg gaaaggcggt cagcaccacg cagggcgggc tgacgcgcag
     2821 tatggagtac gatgctgccg gacgtgtcat cagtctgact aacgagaacg gcagtcacag
     2881 cgacttcagc tacgatgcgc tggaccggct ggtacagcag ggcggctttg acgggcggac
     2941 gcaacgttat cattatgacc tgaccggaaa actcacgcag agtgaggatg aggggcttgt
     3001 caccctctgg cactacgatg cgtcggaccg catcacgcac cggacggtga acggcgaccc
     3061 ggcagagcag tggcagtatg acggccacgg ctggctgaga gaaatcagcc acctgagcga
     3121 aggccaccgt gtcgctgtcc actacggcta cgacgataag ggccgcctga cgggcgaacg
     3181 gcagacggtg gagaacccgg agacggggga actgctgtgg cagcatgaga cgaaacacgc
     3241 ctacaacgaa caggggctgg caaaccgcgt cacgccggac agcctgccgc cggtggagtg
     3301 gctgacctac ggcagcggtt acctggcggg catgaagctg ggcgggacgc cgctggtcga
     3361 gtatacgcgg gacaggctgc accgtgagac ggtgcgcacc ctcgcaccgg acctggacat
     3421 ccgcatcccg tatgccaccg acccggcggg aaaccgcctg ccggacccgg agctacaccc
     3481 ggacagcacg ctcacggcgt ggccggataa ccgcatcgca gaggatgcgc actatgttta
     3541 ccactatgat gaatacggca gactgacgga gaagacggac cgcatcccga cgggtgtgat
     3601 acggacggac gacgagcgga cccaccacta cgacagccag caccgcctgg tgttccacac
     3661 gcggatacag catggcgagc cactggtcga gagccgctac ctctacgacc cgctgggacg
     3721 gcgaatggcg aaacgggtat ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc
     3781 gcgtaaaccg gaggagacat ggtacggctg ggacggcgac aggctgacga cggtacagac
     3841 cgacaccaca cgtatccaga cggtatacga gccgggaagc ttcacgccgc tcatccgggt
     3901 cgagacagag aacggcgagc gggagaaagc gcagcgccgc agcctggcgg agaagctgca
     3961 gcaggaaggg agcgaggacg ggcacggcgt ggtatttccg gctgaactgg tgcggctgct
     4021 ggacagactg gaggaagaaa tccgggcaga ccgcgtgagc agtgaaagcc gggtgtggct
     4081 tgcgcagtgc gggctgacgg tggagcaact ggccagacag gtggagccgg aatacacacc
     4141 agtgcgaaac gttcatcttt accactgcga ccatcggggc cagccgctgg cgctcatcag
     4201 cgaagacggc gatacggtgt ggagcggggc gtatgatgag tggggcaacc agctaaatga
     4261 ggagaacccg catcatgtgt atcagccata ccgtctgccg gggcagcagt atgatgagga
     4321 gtcggggctg tactacaatc gtcaccggta ctatgatccg ttgcagagac ggtacatcac
     4381 ccaggacccc attgggctga aaggtgggtg gaatctgtat cagtatccgc tgaatccggt
     4441 gagcaggata gatccgttgg ggttattctc atgtgagagt tgtagtcatt ttatagatga
     4501 tataaataat aaactggata accttccgtt taacccgaca aaaatgttta cagctagttt
     4561 aaatactgct aattcagcaa ggctgtatgc aagtggcatg acaaaattcg gtataagtgt
     4621 tgctgccgat ggaacgatca ttgctgcccc agtcggtgca gcttctatgg ctttaggggc
     4681 gtggaatata aaatctgcga cagctgcata taacagagca aatttacaat tatcagaatc
     4741 acttcaggaa aatggtagag ataggtgttg gagaaattta ttgggtattt taccttatgg
     4801 tgagcagttt gatgaccctg gtgaaccatc aatattagac gtttatggtg aaaagattga
     4861 gaatatcaaa gattctccaa tgaagttttt tgaagagctt gggacgctgg ggttgtaatg
     4921 tgtattttta atgatatttt tatgtttgtg tttattgtta gtagtatact tgtaaccgtg
     4981 cattttgtgt attctttatt ttttaaaagt ggaaaggtaa aagataaggt aaaaaaatgg
     5041 attaaagtaa tgtgtgattt attttggggg ataggatagt ggtgtgatat ggataaatat
     5101 aaattacagt ggctggcata tttcgacctg acgccggagt agataaaaaa acagacagga
     5161 ttggagtata gcaagcgtct gacaatctct cgggacagta gtatgatgaa gaggcggggt
     5221 tgtactataa ccgtaaccgg tactacgatc cgctaaaggg caagtatatg actcagggaa
     5281 agatcatcag aagttcactt tttgtactaa ataattcgca ttttatgttt aaaaattgag
     5341 atattcctca ttacctgaag ctgttttttt attgcttata cataatcaaa tactccttac
     5401 ataattaagg agaacaaaat ggaaattaaa aaattgatgg aa
//
LOCUS       DAJVYM010000195         5420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVYM010000195 DAJVYM010000000
VERSION     DAJVYM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.197"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OQS60_003646"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OQS60_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS2389298.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OQS60_003647"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OQS60_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS2389299.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OQS60_003648"
     CDS             2408..3157
                     /locus_tag="OQS60_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS2389300.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OQS60_003649"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OQS60_003649"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQS60_003650"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQS60_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS2389301.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..>5420
                     /locus_tag="OQS60_003651"
     CDS             5286..>5420
                     /locus_tag="OQS60_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS2389302.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT         1336 a         1404 c         1382 g         1298 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact agcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt
//
LOCUS       DAJVYM010000196         5336 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVYM010000196 DAJVYM010000000
VERSION     DAJVYM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.198"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1893
                     /locus_tag="OQS60_003652"
     CDS             <1..1893
                     /locus_tag="OQS60_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS2389303.1"
                     /translation="RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSA
                     FNQGSSGSTYYSPDFKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVT
                     GRAQYKNVIDTALSKFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGL
                     AWDMDTQLAWAKLRTTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQ
                     AVDNYTFKTRLDWQKFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNH
                     TIYHKGKGSLGIDNYTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIF
                     ADQTSMITAGYNRYYGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMG
                     LQQKIGKNVIARANYVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFEL
                     AEPLHIRQVDINPQIVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVAD
                     FNNPLKISLNMDFTHQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDE
                     KLDSSLTWDTRLSWTPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWL
                     DVSMKF"
     gene            1950..4733
                     /locus_tag="OQS60_003653"
     CDS             1950..4733
                     /locus_tag="OQS60_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS2389304.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            5095..>5336
                     /locus_tag="OQS60_003654"
     CDS             5095..>5336
                     /locus_tag="OQS60_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2389305.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         1583 a         1199 c         1197 g         1357 t
ORIGIN      
        1 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
       61 gtgaaattgg gttatcgcac tacgcgttcg gactggttaa catcgcatat cgatgagaat
      121 aacaagagcg catttaatca aggctcttca ggaagtactt attactcccc agattttaaa
      181 aagaactttt ataccttgtc gtttaatcag gaactcgctg ataacttcgg cgttaccgcc
      241 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
      301 gtcaccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
      361 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
      421 tataatacca gcaccttccc gcagtctgat cgcgaaatgg gtaataaatc ctatggtctg
      481 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
      541 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
      601 tatggtgata ttacagggcg ttgtacctgt ggcggattag gacacatttc ccaggctgta
      661 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
      721 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
      781 cagtctgaat cctatgtgat taatgctgcc ggaaagaaaa ctaaccatac catttaccat
      841 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga gcacattagc
      901 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
      961 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     1021 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     1081 atccgcaata gctggacgga atcggtatca ggtaataaaa ctctgacgcg ttatcaggat
     1141 ttgaaaacgc cttataacga tgaactggca atgggattgc agcagaaaat cggtaagaac
     1201 gttattgcgc gtgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     1261 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     1321 acgcattcgt tcagcctcag ttttgaactg gccgaacccc tgcatatccg ccaagtagat
     1381 attaacccac aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     1441 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     1501 tacgatagtg ttccagtggc agattttaat aacccattaa agatctcctt aaacatggat
     1561 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     1621 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     1681 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     1741 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     1801 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     1861 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     1921 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     1981 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag gatgaaaagt
     2041 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcaaccaa
     2101 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     2161 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa acatggccgg
     2221 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg tttcggtcgc gatgttaatg
     2281 cctataccag ctatgacgaa acgctgtatc aggtgagtct gccgactacg cagaaacaaa
     2341 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaatac
     2401 tcgaagtaga cgctgaacgc ggcgtaatta ctgaggaatg gcgtgcccat caggatgcga
     2461 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc aaatacccgt aatttagacc
     2521 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     2581 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     2641 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct aacaaagcag
     2701 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     2761 ataaagaaaa ccgagtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     2821 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     2881 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     2941 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     3001 ataatatgca ggatgctgca aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     3061 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     3121 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     3181 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     3241 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     3301 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcttg
     3361 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     3421 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     3481 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccagg gttattctgg
     3541 caaaatccgc gggtgaagag caaaagctac aaattactgc cgtatctaat aaaggcgatt
     3601 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gtaagcggaa
     3661 gcggcgttgg tgagctctcc tcttccagcc tgaaacgctg gagtgcggaa aattcggtaa
     3721 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     3781 accctgaacc tggttttcag ttgattaacc agcgaatcac ccacagcacg attaacgaca
     3841 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     3901 cagcggagaa attcgcccag cagatgtatg agacgcgcta tgctgatgac cgcacgaaat
     3961 tactgcaaga aaatcagatt gtacagttta ctgccgcaga tgcgctggct gccgatcgcc
     4021 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc tcagaagaca
     4081 aactcgtggc gttaattacg cgttacttag gatcaatcaa acactctgat tcgccattag
     4141 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     4201 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     4261 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     4321 atattcgtga acaggcatct ggagcataca gcgtttcttc tcgcctctcg gttgatcctc
     4381 aggccaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     4441 tgttaacgtt agcgaatgaa gtgatggtta agcgtctggc taaagggatc agtgagcaag
     4501 aactgaatga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     4561 aacaattagc gaacactatt gtaaatagtc ttattcaata tgacgatcct gcagcatgga
     4621 ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact gccgttaaac
     4681 aatatctttc tcatccggta aatacttata ctggagtatt attgccaaaa taataacagc
     4741 cccgtcaaca catcgttggc ggggatttta gcaatattcg ccatttttat gtaataattt
     4801 tataaatgcg ttcaaaataa taaccaggta ctaatagtga tattttaagg tctgattttt
     4861 acgtgaaaat tcaggaggca cagaatgcgc ataaaaataa cagcataaaa caccttacca
     4921 ccacccaaga atttcatatt atattgtttt tcaatgaaaa aatattattc tcgtaatatc
     4981 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
     5041 ttatcgataa atcctacttt tttaatgcta tccaatcatt ttaaggagtt taaaatggat
     5101 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
     5161 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
     5221 ttccagatta tcaatgacga attatatctt gatggcaacg ctcgtcagaa cctggccact
     5281 ttctgccaga cctgggacga cgaaaatgtc cacaaattga tggatttatc cattaa
//
LOCUS       DAJVYM010000197         5308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVYM010000197 DAJVYM010000000
VERSION     DAJVYM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.199"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            268..615
                     /locus_tag="OQS60_003655"
     CDS             268..615
                     /locus_tag="OQS60_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS2389306.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            668..1063
                     /locus_tag="OQS60_003656"
     CDS             668..1063
                     /locus_tag="OQS60_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS2389307.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1104..1847
                     /gene="cmoA"
                     /locus_tag="OQS60_003657"
     CDS             1104..1847
                     /gene="cmoA"
                     /locus_tag="OQS60_003657"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS2389308.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1844..2815
                     /gene="cmoB"
                     /locus_tag="OQS60_003658"
     CDS             1844..2815
                     /gene="cmoB"
                     /locus_tag="OQS60_003658"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS2389309.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2980..>5308)
                     /gene="torZ"
                     /locus_tag="OQS60_003659"
     CDS             complement(2980..>5308)
                     /gene="torZ"
                     /locus_tag="OQS60_003659"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS2389310.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1205 a         1374 c         1287 g         1442 t
ORIGIN      
        1 cagtttaccc tgaaaagaga cttcacagat tctttatata tttcaataag ttatgctaaa
       61 aagagactat accgataccg catagaattt gtgtaggatt gcatagatac ttggaaaaac
      121 accgttaaat tgcatagtca ttcacctgat attgtcctgt ttttacaggt agttgattcg
      181 gatgttctag gaatcatcct catagttgat tcggttagtt gggcttgtac caacccctaa
      241 tcaacctcat agtgtattta gaccttgatg attttagaca gccgccctgt tcatgcagca
      301 cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat
      361 gctgtactga cggcgacaaa tccgctgatt cgttttatcg gtagtgatga tatgacgcaa
      421 aaccgggaat tatttcaggt ctggttacag aaattagcgc agtggcatca gaccactacg
      481 ccttatcttt ttttacatac gccagacatt gcccaggccc cggaactggt acataccctg
      541 tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa
      601 tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg
      661 agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc
      721 ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag
      781 cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat
      841 tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg
      901 catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg
      961 ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa
     1021 tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt
     1081 tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg
     1141 ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc
     1201 agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct
     1261 tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct
     1321 cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg
     1381 cgatgattga acgctgcagt cgtcatattg acgcctataa agcccctacg ccagtagacg
     1441 tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt
     1501 ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag
     1561 gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag
     1621 ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac
     1681 tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa
     1741 cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct
     1801 ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa
     1861 cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca
     1921 gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacgcggt
     1981 ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga
     2041 aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct
     2101 gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg
     2161 ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat
     2221 tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca
     2281 cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa
     2341 actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc
     2401 actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct
     2461 ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac
     2521 gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat
     2581 gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg
     2641 ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac
     2701 tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac
     2761 agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc
     2821 tggtattact gccggatgcg gcgtgaatgc cttatccggc ctacaaaccc ttgctaattc
     2881 aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt
     2941 gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt
     3001 atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga
     3061 gttaccggca caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc
     3121 actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac
     3181 cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt
     3241 ccagacacgc accagatcgc cattcgcaat actaaatcga gcagcatctt cggtgtgaat
     3301 agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg
     3361 actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt
     3421 acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa
     3481 tttttccagc gttttggagt aaatctcaat tttgccactt ggcgtaccca gcgcattttt
     3541 caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg
     3601 catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg
     3661 cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt
     3721 accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac
     3781 atcaaaatcg tttcgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg
     3841 ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg
     3901 taatacgata tctgcgtgtt tagcagctgc ggtccagtag cattcagaaa cgacgatcat
     3961 ctccggtttc tgccatgctt taatcagacg attagtatcc tggtgatggg taaagttacc
     4021 gccacccgcc caccagatca ttttgatatt cggatacgtt tgctctttac cgttatgctg
     4081 atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccctcat
     4141 tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag
     4201 cacgccgcca acacgtgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc
     4261 gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt
     4321 ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc
     4381 cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc
     4441 ccaggccgcg ctcttcggcg tattatcgct cttacctctc agatactctt caaactgcgg
     4501 atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt
     4561 atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc
     4621 attatcgcca aagaagtcga tggtttcgga gcggatagga tcaatggcaa tcactggttt
     4681 gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc
     4741 aatttttaag gtattcagcg ggttcatccc ccacagcacc acaacctggc tgttttccag
     4801 aatcagcggc caggaggtct gctgttcata cacctctaca gaaccgacca catgcggcat
     4861 gatcacctgt gccgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag
     4921 gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc
     4981 gtatgatccg gcaaaaatag ccgatggtcc gttagctttg cgaatgcgat cgtgttgctc
     5041 atgaatcagt tttaatgctt gttcccagct cacctgtaca taggtatctt cgccacgacc
     5101 tttcgtcggt tgcagtggat tatcgagata gctttttctc accatcggat gctgaatacg
     5161 cgtcgtggtg tgtacctgat ccgccgccgt agactgtaag gaattcggta tggttttcgc
     5221 cagcgcgcct gttgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca
     5281 acgttccgcg gtgaggattt taccgtcc
//
LOCUS       DAJVYM010000198         5219 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVYM010000198 DAJVYM010000000
VERSION     DAJVYM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.200"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(291..575)
                     /locus_tag="OQS60_003660"
     CDS             complement(291..575)
                     /locus_tag="OQS60_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389311.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="OQS60_003661"
     CDS             complement(593..790)
                     /locus_tag="OQS60_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389312.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="OQS60_003662"
     CDS             1002..1298
                     /locus_tag="OQS60_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389313.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="OQS60_003663"
     CDS             complement(1843..2076)
                     /locus_tag="OQS60_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS2389314.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="OQS60_003664"
     CDS             complement(2083..2613)
                     /locus_tag="OQS60_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS2389315.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2591..3349)
                     /locus_tag="OQS60_003665"
                     /pseudo
     CDS             complement(2591..3349)
                     /locus_tag="OQS60_003665"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="OQS60_003666"
     CDS             complement(3291..3614)
                     /locus_tag="OQS60_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS2389316.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="OQS60_003667"
     CDS             3679..3870
                     /locus_tag="OQS60_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS2389317.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1374 a         1425 c         1114 g         1306 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaatactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctaa cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcacgtcagc gggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg atggtatgaa aagtgcgcca taccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca gccgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ttaccgttta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcct ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAJVYM010000199         5114 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVYM010000199 DAJVYM010000000
VERSION     DAJVYM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.201"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..379
                     /locus_tag="OQS60_003668"
     CDS             <1..379
                     /locus_tag="OQS60_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389318.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OQS60_003669"
     CDS             complement(575..2125)
                     /locus_tag="OQS60_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS2389319.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OQS60_003670"
     CDS             2297..2743
                     /locus_tag="OQS60_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCS2389320.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OQS60_003671"
     CDS             complement(2937..3872)
                     /locus_tag="OQS60_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS2389321.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OQS60_003672"
     CDS             4384..>5114
                     /locus_tag="OQS60_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389322.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJVYM010000200         5089 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVYM010000200 DAJVYM010000000
VERSION     DAJVYM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.202"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..343)
                     /gene="bamD"
                     /locus_tag="OQS60_003673"
     CDS             complement(<1..343)
                     /gene="bamD"
                     /locus_tag="OQS60_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS2389323.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGL"
     gene            478..1458
                     /gene="rluD"
                     /locus_tag="OQS60_003674"
     CDS             478..1458
                     /gene="rluD"
                     /locus_tag="OQS60_003674"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS2389324.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1455..2186
                     /gene="pgeF"
                     /locus_tag="OQS60_003675"
     CDS             1455..2186
                     /gene="pgeF"
                     /locus_tag="OQS60_003675"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS2389325.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2316..4889
                     /gene="clpB"
                     /locus_tag="OQS60_003676"
     CDS             2316..4889
                     /gene="clpB"
                     /locus_tag="OQS60_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS2389326.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1270 a         1213 c         1390 g         1216 t
ORIGIN      
        1 tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta aggcgaataa
       61 aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag gcgtagatga
      121 gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct aacgcttcca
      181 gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca gtcgcgtaaa
      241 tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc gccaaaaaca
      301 ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt tgactttcct
      361 caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc tacaatagca
      421 cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc agtatatatg
      481 gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca acgcttagat
      541 caggctttgg ccgaaatgtt cccggattat tcacgttcgc ggataaaaga atggatcctc
      601 gaccagcgcg tgctggttaa cggcaaagtt tgtgataagc cgaaagaaaa agtattgggt
      661 ggcgagcagg ttgccatcaa cgctgagatt gaagaagaag cgcgttttga accgcaggat
      721 atcccgctgg atatcgtcta tgaagatgaa gatatcattg tcattaataa accgcgcgac
      781 ctagtagtac atcctggcgc gggtaacccg gatggcacgg tactgaatgc gttgcttcat
      841 tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct ggataaagac
      901 accaccggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt agtcgaatct
      961 ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg cgattggtca tatgaccgct
     1021 ggcggtacag tagatgaacc catcagccgc cacccgacca aacgtactca catggcggtg
     1081 catccgatgg gcaaaccggc ggtgactcac tatcgcatca tggaacactt ccgtgtgcac
     1141 acgcgtctgc ggttgcgtct ggaaactgga cgtacgcacc agatccgcgt gcatatggcc
     1201 catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg tccgccaaaa
     1261 ggtgcttcgg aagcatttat ctccacgctg cgtaagtttg accgccaggc gcttcatgca
     1321 accatgctgc gtctttatca cccgatctct ggcatcgaaa tggaatggca tgcgcctatt
     1381 ccacaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga gcataaggat
     1441 gaagtggact ggttatgagt aagctgattg tcccgcagtg gccactgcca aaaggtgttg
     1501 cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgac tcactcaacc
     1561 tcggtgccca ttgtggcgat aacccggatc acgttgagga gaatcgtaag cgactttttg
     1621 ctgcgggcaa tttgccttct aaaccggtct ggcttgagca ggtacacggc aaagatgtgc
     1681 ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat agcaatacgc
     1741 ctggcacggt ttgcgcagtg atgactgccg actgcctccc tgtgctgttt tgcaatcgag
     1801 cgggaacgga agtcgccgcc gttcatgctg gctggcgtgg actgtgcgca ggcgtgctgg
     1861 aagagacggt ttcctgtttt gctgataaac ctgaaaacat tctcgcctgg ttagggccgg
     1921 caattggtcc acgcgcgttc gaagtggggg cggaggttcg cgaggcgttt atggcagtag
     1981 acgctaaagc aagtgcagct ttcattcagc atggtgataa gtatctggcg gatatttatc
     2041 agcttgcccg gcagcgtctg gcgaacgtgg gtgttgagca aattttcggc ggcgaccgtt
     2101 gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc accggtcgta
     2161 tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt acgcgtgatt
     2221 ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc tcatttaatc
     2281 tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt cttactaata
     2341 aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac aaccaattca
     2401 tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg gttagtcctt
     2461 tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat caggcattaa
     2521 atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag gatctggtgc
     2581 gcgttcttaa tctttgcgac aagctggcgc aaaaacgagg tgataacttt atctcgtcag
     2641 aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc ctgaaagcag
     2701 caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga ggtgaaagcg
     2761 tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc atcgacctta
     2821 ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa gaaattcgcc
     2881 gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt ggtgaacccg
     2941 gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac ggcgaagtgc
     3001 cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg gtggctgggg
     3061 cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat cttgccaaac
     3121 aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc gcgggtaaag
     3181 ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt ggtgaattgc
     3241 actgcgtagg tgccacgacg cttgacgaat atcgccagta cattgaaaaa gatgctgcgc
     3301 tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat accattgcga
     3361 ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt actgacccgg
     3421 caattgttgc agcggcgacg ttgtctcatc gctacattgc tgaccgtcag ctgccggata
     3481 aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt gactcaaaac
     3541 cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa caacaggcgt
     3601 taatgaaaga gtctgatgaa gccagtaaaa aacgcttgga tatgctcaac gaagaactga
     3661 gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag aaggcatcgc
     3721 tttctggtac gcagaccatt aaagcggaac tggaacaggc gaaaatcgct attgaacagg
     3781 ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa atcccggaac
     3841 tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt ctgttgcgta
     3901 ataaagtgac cgacgccgaa attgctgaag tgctggcgcg ttggacgggg attccggttt
     3961 ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa ctgcaccatc
     4021 gcgtcattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgc cgtagccgtg
     4081 cggggttggc ggatccaaat cgcccgattg gttcattcct gttcctcggc ccaacgggtg
     4141 tggggaaaac tgagctttgt aaggcgctgg cgaactttat gtttgatagc gacgaggcga
     4201 tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt ctggttggtg
     4261 cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct gtgcgtcgtc
     4321 gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat gtcttcaaca
     4381 ttctgttgca ggtactggat gatggtcgtc tgactgacgg gcaggggaga acggtcgact
     4441 tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt caggaacgct
     4501 tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta agccataact
     4561 tccgtccgga attcattaac cgtattgatg aagtggtggt cttccatccg ctgggtgaac
     4621 agcacattgc ctcgattgcg cagattcagt tgaaacgtct gtacaaacgt ctggaagaac
     4681 gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag aacggttacg
     4741 atccagtcta tggtgcacgt cctttgaaac gtgctatcca gcagcagatc gaaaacccgc
     4801 tggcacagca aatactgtct ggtgaattgg ttccgggtaa agtgattcgc ctggaagtta
     4861 atgaagaccg gattgtcgct gtccagtaat gataaaacga gcccttcggg gctcgttttt
     4921 gtctataagt tagacggaaa agactatatt taagatgttt tgcctgaaaa atgagcgaac
     4981 gataaagttt ttatattttt cgcttgtcag gccggaataa ctccctataa tgcgccacca
     5041 ctgacacgga acaacggcaa acacgccgcc gggtcggcgg ggttctcct
//
LOCUS       DAJVYM010000201         4925 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVYM010000201 DAJVYM010000000
VERSION     DAJVYM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.204"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..929)
                     /gene="tuf"
                     /locus_tag="OQS60_003677"
     CDS             complement(<1..929)
                     /gene="tuf"
                     /locus_tag="OQS60_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS2389327.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEV"
     gene            complement(1044..1119)
                     /locus_tag="OQS60_003678"
     tRNA            complement(1044..1119)
                     /locus_tag="OQS60_003678"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1084..1086),aa:Thr,seq:ggt)
     gene            complement(1126..1200)
                     /locus_tag="OQS60_003679"
     tRNA            complement(1126..1200)
                     /locus_tag="OQS60_003679"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1165..1167),aa:Gly,seq:tcc)
     gene            complement(1317..1401)
                     /locus_tag="OQS60_003680"
     tRNA            complement(1317..1401)
                     /locus_tag="OQS60_003680"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1365..1367),aa:Tyr,seq:gta)
     gene            complement(1410..1485)
                     /locus_tag="OQS60_003681"
     tRNA            complement(1410..1485)
                     /locus_tag="OQS60_003681"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:Thr,seq:tgt)
     gene            1847..2797
                     /gene="coaA"
                     /locus_tag="OQS60_003682"
                     /gene_synonym="panK"
     CDS             1847..2797
                     /gene="coaA"
                     /locus_tag="OQS60_003682"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS2389328.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2826..3791)
                     /gene="birA"
                     /locus_tag="OQS60_003683"
     CDS             complement(2826..3791)
                     /gene="birA"
                     /locus_tag="OQS60_003683"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS2389329.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3788..4816)
                     /gene="murB"
                     /locus_tag="OQS60_003684"
     CDS             complement(3788..4816)
                     /gene="murB"
                     /locus_tag="OQS60_003684"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS2389330.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1249 a         1257 c         1117 g         1302 t
ORIGIN      
        1 tacacttcag attcgaactt ggtgtgcggc ttgatggtgc ccggcttagc cagtacctga
       61 ccacgttcga tttcttcacg tttgatacca cgcagcagaa cacctacgtt ctcaccagca
      121 cggccttcgt ccagcagttt gcggaacatt tcaacgccag tacaggtaga cttctgagtc
      181 tctttgatac caacgatttc aacttcttca ccaactttga tgataccgcg ttctacacga
      241 ccggtaacaa cggtaccacg accggagatg gagaatacgt cttcgatcgg cagcaggaac
      301 ggcttgtcaa tcgcacgctc tggttccgga atgtaagaat ccaggaagcc agccagttcc
      361 aggattttcg cttcccactc tgcgtcgcct tccagcgctt tcagagcaga accacgaacg
      421 atcggagtgt cgtcgcccgg gaagtcgtac tgagacagaa gttcacgaac ttccatttca
      481 accagttcca gcagctcttc gtcatcaacc atgtcgcatt tgttcaggaa cacgatgatg
      541 tacggaacgc ctacctgacg acccagcagg atgtgctcac gagtctgcgg catcgggccg
      601 tcagtcgcag caactaccag gatcgcgccg tccatctgag cagcaccggt gatcatgttt
      661 ttaacatagt cggcgtgccc cgggcagtct acgtgtgcgt agtgacgggt cggagtgtcg
      721 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
      781 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      841 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      901 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      961 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
     1021 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
     1081 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
     1141 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
     1201 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
     1261 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
     1321 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
     1381 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
     1441 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
     1501 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
     1561 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
     1621 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
     1681 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1741 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1801 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1861 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1921 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1981 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     2041 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     2101 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     2161 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     2221 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     2281 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     2341 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     2401 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     2461 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     2521 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     2581 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     2641 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     2701 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2761 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2821 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2881 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2941 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     3001 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     3061 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     3121 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     3181 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     3241 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     3301 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     3361 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     3421 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     3481 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     3541 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     3601 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     3661 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3721 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3781 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3841 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3901 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3961 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     4021 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     4081 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     4141 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     4201 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     4261 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     4321 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     4381 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     4441 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     4501 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     4561 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     4621 ggttgatgat caccgtgcca cgatagtctt ccagaaaaag tacattactt ccttcaccca
     4681 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4741 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4801 ttaaggagtg gttcatagct gctttcctga tgcaaaaaca aggctagttt accgtatctg
     4861 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4921 ccgtc
//
LOCUS       DAJVYM010000202         4921 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVYM010000202 DAJVYM010000000
VERSION     DAJVYM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.205"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1194)
                     /gene="dtpA"
                     /locus_tag="OQS60_003685"
     CDS             complement(<1..1194)
                     /gene="dtpA"
                     /locus_tag="OQS60_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS2389331.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELM"
     gene            complement(1805..2440)
                     /gene="nth"
                     /locus_tag="OQS60_003686"
     CDS             complement(1805..2440)
                     /gene="nth"
                     /locus_tag="OQS60_003686"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS2389332.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWFILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2440..3135)
                     /gene="rsxE"
                     /locus_tag="OQS60_003687"
     CDS             complement(2440..3135)
                     /gene="rsxE"
                     /locus_tag="OQS60_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS2389333.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3139..3759)
                     /gene="rsxG"
                     /locus_tag="OQS60_003688"
     CDS             complement(3139..3759)
                     /gene="rsxG"
                     /locus_tag="OQS60_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS2389334.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(3763..4821)
                     /gene="rsxD"
                     /locus_tag="OQS60_003689"
     CDS             complement(3763..4821)
                     /gene="rsxD"
                     /locus_tag="OQS60_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS2389335.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         1299 a         1248 c         1208 g         1166 t
ORIGIN      
        1 catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta cagacacgat
       61 accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat
      121 caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct tgttatagat
      181 agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct gatactgctc
      241 aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa agaagttcag
      301 tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca tcaggatgaa
      361 ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt taccgaagat
      421 aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag
      481 cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca gcaggttacg
      541 gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc gttggcagaa
      601 ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca
      661 gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc cgatgttgac
      721 ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct catagcatgt
      781 agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat
      841 acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc
      901 cagcacaata gcaccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca
      961 gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga
     1021 atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc
     1081 gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg
     1141 tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac
     1201 ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata
     1261 cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca
     1321 gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg
     1381 gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt tcactgcact
     1441 gagaggggcg tgatggctta acggcgatat gttctgctat ttagcgtttt tactagtgag
     1501 atagtccagt ttctgaaaaa tagccagtgt aatgttttgt aggtcaatat ttgttgcgtg
     1561 tgtaaattta aattatttta acctgattct tgcaatgttt ttacattgcg acagcatcgt
     1621 gatcttgatc acgaatgaat agaaaatttc gctattaaaa aaacaattaa cctgcatttt
     1681 tggttatccg caaacggatt acctgatgat aacgctaagc aatggcatca ttcacgttaa
     1741 aaagatgttt taaaagagga taaagaaagg ttatcaatgg ggtaatcggt gttacccctt
     1801 ttcttcagat gtcaactttc tctttgtatt cacaaagatc ttcaataata caagagccac
     1861 agcggggctt gcgggcaatg caggtataac gcccgtgcag gataaaccaa tggtggcagt
     1921 cgactttaaa ctctgccgga accactttca gtagcttttc ttctacctgt tcgacgtttt
     1981 tccccggcgc aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag
     2041 tcggccagcc gaatgcagtg tttaatacga cgttggctgt tttacgacct acgccgggca
     2101 gggcttcaag cgcagcacga tcttccggaa cctcgccatt atgctgctcc agtaagatac
     2161 ggcaggtttt gatgatattt tctgctttgc tgttataaag cccaatcgtt ttgatatagg
     2221 ttttaacccc ttcaacgccc agttcaagca tcgctgcagg cgtattcgcc accgggtaaa
     2281 gtttcgccgt cgccttatta acactgacat cggtcgcctg agcggaaagc agtacggcaa
     2341 tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga ttgttctcac
     2401 gcaggcgagt gaggatctcc aggcgttttg ctttgttcat cagacattcc ctgtttcacc
     2461 gtttggcaat gcacgttcgg cagctgcttc agtacggcgc tttttcattc tttcatcaat
     2521 caggtatttt cctgccagca tcagtcccag gccaataaat gcacctggtg gcagcatcgc
     2581 cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg cccagctacc
     2641 taacagcgca tctgcaccgt caaacaatgt gccattgccg ataatttcgc gtagtgaacc
     2701 cagcacgaac atggcgcagg ttgcgcccat accaattgaa aagccgtcca gtgccgaaag
     2761 cgccggacct tttttggcgg cgaaggcttc agcgcggccc acaacgatac agttagtgac
     2821 aatcagcgga ataaaaatcc ctaatgattg atacaggcca aaggcgtagg cgttgatcag
     2881 catctgtaca gcgctgacca ccgaggcgat gatcatcacg taaatgggaa tgcggatctc
     2941 ggctggcgtc cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt
     3001 agtcgcaagt cctaaaccca gagcgttagt ggcagtggac gtgaccgcca acagaggaca
     3061 aaggccgagc aactggacca gcgcagagtt gtttttccac aacccctgaa caataacgtc
     3121 tttaatttcg ctcacgcttt attctccaca ggcaggaagt tgagaaagtt gtgccggtaa
     3181 cgtctgagcg tacaatccgg cgcgttttac cgcattaacc accgcgcggg gagtaatcgt
     3241 cgcgccggtg aactggtcga aatcaccacc atctttcttc accgcccagt gcgcatcatc
     3301 cgcaccactg atttttttac ccgcaaaatg ggtgatccag tcagaaaggc gcagttcgat
     3361 tttatcgcca agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc
     3421 gttaaaatcg gctcccacca gcagctgaat cgcaccggaa tagccatctg gcgcggttgc
     3481 ttccagaacg gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc
     3541 tttacctaac tctggcgcag ttaccagata gcaactctgt gccagcgtat tgttatagcg
     3601 ttcggctggc agcacctgat caaataatgc cttttgttgc agactggcct gttcagcaat
     3661 cgtcgttttg gtcatctggt tgatggccgc agttaaccct gttgaacccg ctgcaaatag
     3721 cgctaacgta atgccgtgtt ttcggatagt tttcagcatg gtttaccctt tgcgatggcc
     3781 gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg ccagcaggac
     3841 ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg atcaaccaga ctaataagcc
     3901 cgcaagcgcg ccgaaaataa gacgaccacg attggtcgta gaagcggtaa ccgggtcagt
     3961 caaaataaag aatgcgccga gcatggtcgc accagacagc agatgaattt gcggtgctgc
     4021 cagtgtttct ggtgagaaca accagcccaa cgttgcgcat aacgccagcg ttactaagaa
     4081 gctgagggga atatgccagc gaatcgcttt ctgccatagc aaccacaggc cgccagccag
     4141 ccaggcgaga tttacccatt gccaaccagc gcccgccaga ataccgctgt agatcggata
     4201 ttgcataatc tgttcaaccg aatgaccggc acggacagag gttttaaatg tatccagcgg
     4261 tgtcgcctga ctaatgccat caatacctaa gcgtagtgtg ttcatatcac caccactggc
     4321 ggtatgcccg ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat
     4381 ttcatgtggc ggtaaccagc tggtcatctg caccgggaag gagatcagta agaccacata
     4441 accaatcatt gccggattaa acgggttttg ccccagaccg ccatacaact gtttagcgat
     4501 gatcaccgca aacaccgtac ccagcacgac catccaccat ggcgcgaggg ggggaatact
     4561 taccgccagc aataagcctg tcagcaatgc tgagttatct ttcaacgttg cggctaccga
     4621 ctgcttgcgt agtttgagta cgagagcttc ggctaacaga gtactaaccg atgccaacag
     4681 gatctgaacg agagtacccc aaccaaaaaa ccacagttgc gctgcgattc ctggcacggc
     4741 tgcgagcaac accaacagca taatgcgcga tgtctggcgc tggttatggg tataagggga
     4801 gctagctatt ctgaatacca tttagtcctc gtttacaacc ttctgctggg cggctttttt
     4861 ggcctgagcg cgggcaatag ccgcggcaac tgccgctttg cgcggatcaa cctgttcttc
     4921 t
//
LOCUS       DAJVYM010000203         4803 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVYM010000203 DAJVYM010000000
VERSION     DAJVYM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.206"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(279..1322)
                     /locus_tag="OQS60_003690"
     CDS             complement(279..1322)
                     /locus_tag="OQS60_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS2389336.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="OQS60_003691"
     CDS             complement(1734..2918)
                     /locus_tag="OQS60_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2389337.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="OQS60_003692"
     tRNA            complement(3218..3312)
                     /locus_tag="OQS60_003692"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4803
                     /locus_tag="OQS60_003693"
     CDS             3605..>4803
                     /locus_tag="OQS60_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS2389338.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1055 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgc
//
LOCUS       DAJVYM010000204         4798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVYM010000204 DAJVYM010000000
VERSION     DAJVYM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.207"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(74..517)
                     /locus_tag="OQS60_003694"
     CDS             complement(74..517)
                     /locus_tag="OQS60_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS2389339.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            674..1603
                     /gene="metA"
                     /locus_tag="OQS60_003695"
     CDS             674..1603
                     /gene="metA"
                     /locus_tag="OQS60_003695"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS2389340.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1872..3473
                     /gene="aceB"
                     /locus_tag="OQS60_003696"
                     /pseudo
     CDS             1872..3473
                     /gene="aceB"
                     /locus_tag="OQS60_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3503..4794
                     /gene="aceA"
                     /locus_tag="OQS60_003697"
                     /pseudo
     CDS             3503..4794
                     /gene="aceA"
                     /locus_tag="OQS60_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1197 a         1261 c         1216 g         1124 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc gggtgcgacg ctggcgcgtc ttctccagcc tgggcaggca
       61 cggcaggggc agcttattcc tccacatacg ccagattcag caacggatac ggtttcccca
      121 aatcgtccac ctcagagcgt cccgtaacct taaaacccac cttcttatag aacccaaccg
      181 cctgctcatt ttgctcatta acgttggttg tcagttccgg tgccatcgag agcgcatgct
      241 ccaccagcat ccgacccacg ccgcagccgc acacatcagg atcgataaac agcgcatcca
      301 tatgctgccc acttaacaac ataaacccaa ccggctgatc ccgttcatta accgcgaccc
      361 acaacggcgc ttccggcagg aaggaacgaa ctagcacctc cagctcggcc cgatactctg
      421 ctgataaaaa atcgtgtgtg gcatcgacag aacgacacca aatcgcaacg agttcctccc
      481 cttcctcatg ccgtgagcgg cgaatactaa taaccatttt ctctcctttt cgtcattctt
      541 atattctaac gtagcctttt ccttgaaact ttctcacctt caacatgcag gctcgacatt
      601 ggcaaatttt ctggttatct tcagctatct ggatgtctaa acgtataagc gtatgtagtg
      661 aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta cccgccgtca atttcttgcg
      721 tgaagaaaac gtctttgtga tgacaacttc tcgtgcgtct ggtcaggaaa ttcgtccact
      781 taaggttctg atccttaacc tgatgccgaa gaagattgaa actgaaaatc agtttctgcg
      841 cctgctttca aactcacctt tgcaggtcga tattcagctg ttgcgcatcg attcccgtga
      901 atcgcgcaac acgcccgcag agcatctgaa caacttctac tgtaactttg aagatattca
      961 ggaacaaaac tttgacggtc tgatagttac cggcgcgccg ctgggcctgg tggagtttaa
     1021 tgatgtcgct tactggccgc agatcaaaca ggtgctggag tggtcgaaag atcacgtcac
     1081 ctcgacgctg tttgtctgct gggcggtaca ggccgcgctc aatatcctct acggcattcc
     1141 taagcaaact cgcaccgaca aactctctgg cgtttacgag catcatattc tccatcctca
     1201 tgcgcttctg acgcgtggct ttgatgattc attcctggca ccgcattcgc gctatgctga
     1261 ctttccggca gcgttgattc gtgattacac cgatctggaa attctggcag agacggaaga
     1321 aggggatgca tatctgtttg ccagtaaaga taagcgcatt gcctttgtga cgggccatcc
     1381 cgaatatgat gcgcaaacgc tggcgcagga atatttccgc gatgtggaag ccggactaga
     1441 cccggatgta ccgtataact atttcccgca caatgatccg caaaacaaac cgcgcgcgag
     1501 ctggcgtagt cacggtaatt tgctgtttac caactggctc aactattacg tctaccagat
     1561 cacgccatac gatctacggc acatgaatcc aacgctggat taatcttctg tgatagtcga
     1621 tcgttaagcg attcagcacc ttacctcagg caccttcggg tgcctttttt atttccgaaa
     1681 cgcacctcag caagtgaata aattttattc atattgttat caataagtta tcaagtattt
     1741 ttaattaaaa tggaaattgt ttttgatttt gcattttaaa tgagtagtct tagttgtgct
     1801 gaacgaaaag cgcacaacga tccttcgttc acagtgggga agttttcgga tccatgacga
     1861 ggagctgcac gatgactgaa caggcaacaa caaccgatga actggctttc acaaggccgt
     1921 atggcgagca ggagaagcaa attcttactg ccgaagcggt agaatttctg actgagctgg
     1981 tgacgcattt tacgccacaa cgcaataaac ttctggcagc gcgcattcag cagcagcaag
     2041 atattgataa cggaacgttg cctgatttta tttcggaaac agcttccatt cgcgatgctg
     2101 attggaaaat tcgcgggatt cctgcggact tagaagaccg ccgcgtagag ataactggcc
     2161 cggtagagcg caagatggtg atcaacgcgc tcaacgccaa tgtgaaagtc tttatggccg
     2221 atttcgaaga ttcactggcc ccggactgga acaaagtgat cgacgggcaa attaacctgc
     2281 gtgatgcagt taacggcacc atcagctata ccaatgaagc aggcaaaatt tatcagctca
     2341 agcccaatcc agcggttttg atttgtcggg tacgcggtct acacttgccg gaaaaacatg
     2401 tcacctggcg tggtgaggca atccccggta gcctgtttga ttttgcgctc tatttcttcc
     2461 acaactatca ggcactgttg gcaaagggca gcggtcccta tttctatctg ccgaaaaccc
     2521 agtcctggca ggaagcggcc tggtggagcg aagtcttcag ctatgcagaa gatcgcttta
     2581 atctgccgcg cggcgccatc aaggcgacgt tgctgattga aacgctgccc gccgtgttcc
     2641 agatggacga aatccttcac gcgctgcgcg accatattgt tggtctgaac tgcggtcgct
     2701 gggattacat cttcagctat atcaaaacgt tgaaaaacta tcccgatcgt gtcctgccag
     2761 acagacaggc cgtgacgatg gataaaccat tcctgaatgc ttactcacgc ctgctgatta
     2821 aaacctgcca taaacgcggt gcttttgcga tgggcggcat ggcagcgttt attccgagca
     2881 aagatgaaga gcgtaataac caggtgctca acaaagtaaa agcggataaa gcgctggaag
     2941 tcaataacgg tcacgatggc acatggatcg ctcacccagg ccttgcggac acggcaatgg
     3001 cggtattcaa cgacattctc ggctcccgta aaaatcagct tgaagtattg cgcgaacaag
     3061 acgcgccgat tactgccgat cagctgctgg caccttgtga cggtgaacgc accgaagaag
     3121 gtatgcgcgc caacattcgc gtggctgtgc agtacatcga agcgtggatc tccggcaacg
     3181 gctgcgtgcc gatttatggc ctgatggaag atgcggcgac ggctgaaatt tcccgtacct
     3241 cgatctggca gtggatccat catcaaaaaa cgttgagcaa tggcaaaccg gtgaccaaag
     3301 ccttgttccg ctagatgctg ggcgaagaga tgaaagtcat tgccagcgaa ctgggcgaag
     3361 aacgtttctc ccagggccgt tttgacgatg ccgcacgctt gatggaacag atcaccactt
     3421 ccgatgagtt aattgatttc ctgaccctgc caggctaccg cctgttagcg taaaccacca
     3481 cataactatg gagcatctgc acatgaaaac ccgtacacaa caaattgaag aattacagaa
     3541 agagtggact caaccgcgtt gggaaggcat tactcgccca tacagtgcgg aagatgtggt
     3601 gaaattacgc ggttcagtca atcctgaatg cacgctggcg caactgggcg cagcgaaaat
     3661 gtggcgtctg ctgcacggtg agtcgaaaaa aggctacatc aacagcctcg gtgcactgac
     3721 tggcggtcag gcgctgcaac aggcgaaagc gggtattgaa gcagtctatc tgtcgggatg
     3781 gcaggtagcg gcggacgcta acctggcggc cagcatgtat ccggatcagt cgctctatcc
     3841 ggcaaactcg gtgccagctg tggtggagcg gatcaacaac accttccgtc gtgccgatca
     3901 gatccaatgg tccgcgggca ttgagccggg cgatccgcgc tatgtcgatt acttcctgcc
     3961 gatcgttgcc gatgcggaag ccggttttgg cggtgtcctg aatgcctttg aactgatgaa
     4021 agcgatgatt gaagccggtg cagcggcagt tcacttcgaa gatcagctgg cgtcagtgaa
     4081 gaaatgcggt cacatgggcg gcaaagtttt ggtgccaact caagaagcta ttcagaaact
     4141 ggtcgcggcg cgtctggcag ctgacgtgac gggcgttcca accctactgg ttgctcgtac
     4201 cgatgccgat gcagcggatc tgatcacctc cgattgcgac ccgtatgaca gcgaatttat
     4261 taccggcgag cgtaccagtg aaggcttctt ccgtactcat gcgggcattg agcaagcgat
     4321 cagccgtggc ctggcgtatg cgccatatgc tgacctggtc tggtgtgaaa cctccacgcc
     4381 ggatctggaa ctggcgcgtc gctttgcaca agctatccac gcgaaatatc cgggcaaact
     4441 gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa aacctcgacg acaaaactat
     4501 tgccagcttc cagcagcagc tgtcggatat gggctacaag ttccagttca tcaccctggc
     4561 aggtatccac agcatgtggt tcaacatgtt tgacctggca aacgcctatg cctagggcga
     4621 gggtatgaag cactacgttg agaaagtgca gcagccggaa tttgccgcta taccttcgta
     4681 tctcaccagc aggaagtggg tacaggttac ttcgataaag tgacgactat tattcagggc
     4741 ggcacgtctt cagtcaccgc gctgaccggc tccactgaag aatcgcagtt ctaagcaa
//
LOCUS       DAJVYM010000205         4677 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVYM010000205 DAJVYM010000000
VERSION     DAJVYM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.208"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1046)
                     /locus_tag="OQS60_003698"
     CDS             complement(<1..1046)
                     /locus_tag="OQS60_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS2389341.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OQS60_003699"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OQS60_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OQS60_003700"
     CDS             complement(2433..3281)
                     /locus_tag="OQS60_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS2389342.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OQS60_003701"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OQS60_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS2389343.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..>4677
                     /gene="tadA"
                     /locus_tag="OQS60_003702"
     CDS             4150..>4677
                     /gene="tadA"
                     /locus_tag="OQS60_003702"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS2389344.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPS"
BASE COUNT         1218 a          992 c         1159 g         1308 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcg
//
LOCUS       DAJVYM010000206         4614 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVYM010000206 DAJVYM010000000
VERSION     DAJVYM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.209"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..1133)
                     /locus_tag="OQS60_003703"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="OQS60_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="OQS60_003704"
     CDS             complement(1133..1315)
                     /locus_tag="OQS60_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389345.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="OQS60_003705"
     CDS             complement(1327..3060)
                     /locus_tag="OQS60_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS2389346.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="OQS60_003706"
     CDS             complement(3057..3551)
                     /locus_tag="OQS60_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS2389347.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="OQS60_003707"
     CDS             complement(3673..4023)
                     /locus_tag="OQS60_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS2389348.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="OQS60_003708"
     CDS             complement(4066..4530)
                     /locus_tag="OQS60_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS2389349.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..>4614)
                     /locus_tag="OQS60_003709"
     CDS             complement(4527..>4614)
                     /locus_tag="OQS60_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS2389350.1"
                     /translation="WTYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT          997 a         1369 c         1156 g         1092 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccag
//
LOCUS       DAJVYM010000207         4595 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVYM010000207 DAJVYM010000000
VERSION     DAJVYM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.210"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(98..856)
                     /locus_tag="OQS60_003710"
     CDS             complement(98..856)
                     /locus_tag="OQS60_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS2389351.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(864..1967)
                     /locus_tag="OQS60_003711"
     CDS             complement(864..1967)
                     /locus_tag="OQS60_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS2389352.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1977..3158)
                     /locus_tag="OQS60_003712"
                     /pseudo
     CDS             complement(1977..3158)
                     /locus_tag="OQS60_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3226..4251)
                     /locus_tag="OQS60_003713"
     CDS             complement(3226..4251)
                     /locus_tag="OQS60_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2389353.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1269 a         1191 c         1085 g         1050 t
ORIGIN      
        1 gggtctcccc atgcgagagt agggaactgc cagacatcaa atcaagcgaa aggccatccg
       61 aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc tgcgataaaa
      121 atgccttcgt acgctctgat ttaggatgcg caaaaaattc atcaggtgca gcttgctcca
      181 ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca aaccccatct
      241 catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc gtatccagca
      301 cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac ataattttcg
      361 gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca gaaatttgtc
      421 cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc atcgccagag
      481 cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta cagttctgta
      541 gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact tcctgcctga
      601 cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctactacg atccgtccct
      661 gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg gaaccagaag
      721 ggccacacag aacgatccgt tctcccagtt gcacggttaa atttatattt ttcaaaacat
      781 ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta gcaggttgca
      841 gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa acgtttttcc
      901 agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag tgcggcgaaa
      961 acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg ctgaacgcta
     1021 ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa gagtgcgatg
     1081 atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat aaccagcccc
     1141 tgagttttcc agtaacccaa tgccagtgac tctgccgctt catattgccc tttaggcagc
     1201 gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag gatcacgcca
     1261 accagcgcgc ggatcagttt gtcgatattg gtgccttctg ccataaacaa aggcagcatg
     1321 accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc gataaaaatg
     1381 accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag taagatcccc
     1441 cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt cagcccgccc
     1501 cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag ccaccagaca
     1561 atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg gagtattttc
     1621 cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat tcgccagcgt
     1681 tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat gaagacccaa
     1741 caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc ctgcaaaaat
     1801 gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc aatagtcagc
     1861 aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc gcggcttgag
     1921 ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat gactccttag
     1981 cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat agtcaggctg
     2041 ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc cgtctggttc
     2101 agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc ggcaagagag
     2161 gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac tcgcagtgcc
     2221 tggggaataa tgacctagcg tagcgtaacc gggttgggta atcccagtga ccgagccgct
     2281 tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc gataaatgca
     2341 gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa aaccatcccg
     2401 ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc gccaaaaagc
     2461 cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca ggtgcggcgc
     2521 agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac agaaaggact
     2581 atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg cggtgacgga
     2641 atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc ctggcgtggt
     2701 ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag cgggggaata
     2761 ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt atccgaaagt
     2821 ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa tgcagaaacc
     2881 agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc ctgctggtaa
     2941 tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc aaaacctgaa
     3001 gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc aacaatgccg
     3061 acaacagcaa caacggcaag gatctggaat aaccaggcac gaaccgtagg gttggcaaaa
     3121 gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga aagcataacg
     3181 ctgaagagag cgccgccgta gcgacgctcg ttacctcaca ccgacttaac gcacgggcgg
     3241 cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa ttttcagcgg
     3301 gctttctgaa cctacgttac gctcgaaaat ttccgagtag ttacccacct gtttgatgat
     3361 gttataggcc catttattat ccagcttcag atccttgccg taatcgcctt ctttacccag
     3421 cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt tctgggaatt
     3481 gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa tcgagaacca
     3541 ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa cctccggtaa
     3601 gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt gcgactgatc
     3661 cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt cagagcgatc
     3721 gaaagtcacc ggtgtgtact tcatattgtt tgccttgaaa tagtcggcga cgttgagttc
     3781 ggtatctgta cccgcctgaa tacagacggt agcgccatcc agttctttcg cgctttttag
     3841 ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa tcagtgacgc cagtaaatgc
     3901 cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga gcaaatccac
     3961 ctcccctgac tgtaaagcgg tgaagcgttc ttttgcagtg agcggggtat atttcacttt
     4021 cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatctacat caatacctga
     4081 aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga tcccgcattg
     4141 cacaaaacct tttttctgta cggcatcaag cgtcgcgcca gcatgcgcct gatttgcaac
     4201 ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca taatgcttcc
     4261 tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct gtctgtggcc
     4321 atcctcttat aagcaaaagt agtgccatct tttgcgcacg atttcgcgtc gcaataagct
     4381 gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat gaaacatgcg
     4441 ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg attgttagcg
     4501 tgagtatatt tctggtggga ggtgtgaaag ttaaattgat gcgttatttc gaaagcagcg
     4561 aaataacgcg atataacatg ctaaggtaat gactc
//
LOCUS       DAJVYM010000208         4552 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVYM010000208 DAJVYM010000000
VERSION     DAJVYM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.211"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..206
                     /locus_tag="OQS60_003714"
     CDS             <1..206
                     /locus_tag="OQS60_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCS2389354.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
     gene            345..1295
                     /locus_tag="OQS60_003715"
     CDS             345..1295
                     /locus_tag="OQS60_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS2389355.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            1522..2970
                     /gene="uxaB"
                     /locus_tag="OQS60_003716"
     CDS             1522..2970
                     /gene="uxaB"
                     /locus_tag="OQS60_003716"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS2389356.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3142..3450)
                     /locus_tag="OQS60_003717"
     CDS             complement(3142..3450)
                     /locus_tag="OQS60_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2389357.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(3444..>4552)
                     /gene="lsrA"
                     /locus_tag="OQS60_003718"
     CDS             complement(3444..>4552)
                     /gene="lsrA"
                     /locus_tag="OQS60_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCS2389358.1"
                     /translation="ADRQMVEILRGLMRDSRILILDEPTASLTPAETERLFRRLQELL
                     ATGVGIVFISHKLPEIRQIADRISVMRDGTIALSGKTSELSTDDIIQAITPVVREKSL
                     SASQKLWLELPGNRPQHAAGTSVLTLENLTGEGFRNVSLTLNAGEILGLAGLVGAGRT
                     ELAETLYGLRTLRGGRIMLNGKEINKLSTGERLLRGLVYLPEDRQSSGLNLDASLAWN
                     VCALTHNLRGFWAKTAKDNATLERYRRALNIKFNQPEQAARTLSGGNQQKILIAKCLE
                     ASPQALIVDEPTRGVDVSARNDIYQLLRSIAAQNVAVLLISSDLEEIELMADRVYVMH
                     QGEITHSALTGRDINVETIMRVAFGDSQRQEASC"
BASE COUNT         1089 a         1104 c         1174 g         1185 t
ORIGIN      
        1 cggcattacc ggctaatgac ggtaagcaaa cccccatgcg cgggcatccg gtgtttatcg
       61 ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat aacattccgc
      121 agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc tatcactggc
      181 tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag cattcccagg
      241 gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa tttacttatt
      301 acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat gtacacccca
      361 tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc gctatttttg
      421 cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac gaactttctc
      481 ttttctggcc gctgaatggc gtaatggcgg gggggtttgc ccgctatgtc tggcttaatc
      541 gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc ataaccaccg
      601 aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt gttaccgtcg
      661 ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca gtaagtgcct
      721 tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc ggggcgattg
      781 gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg ttcagtgagc
      841 aattctcaac gggcgtgttg atcgtgcctc tgacggtcat gctgcttgat atcgactatt
      901 tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta agcgtgtttg
      961 cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg ggcggcgaag
     1021 aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg gcggaaaaaa
     1081 tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt tatctcacgg
     1141 taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat gattttaata
     1201 aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg cgcaaccgta
     1261 ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc cgcgcgattt
     1321 gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct cgattaaacc
     1381 atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag ttgatcaaaa
     1441 caggggttcg gcgtattaaa gtttgctaac ctgtcgtacc aattaaaaca ggttgtatga
     1501 ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc cggtgcacag
     1561 tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt tgttgactgg
     1621 caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt tgttcgtccg
     1681 attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtatac caccattatc
     1741 cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg ctcggttaat
     1801 cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca caacccggaa
     1861 atgcgctttg ttttctctaa caccaccgaa gcggggatca gctaccatgc gggcgataaa
     1921 ttcgatgacg cgccagcgat aagctatccg gcaaaactga cccgtctgct gttcgaacgc
     1981 tttagccatt tcaacggtgc gctggataaa ggttggatca tcatcccgtg tgagttgatc
     2041 gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga gtgggcactg
     2101 ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac gctggtggac
     2161 cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga actgggttat
     2221 cacgatggtt tcctcgacac cgctgaacac ttttacctgt ttgtgattca ggggccgaaa
     2281 tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat tgttgacgat
     2341 attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac cgcgctggtg
     2401 ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga tgctgaaatt
     2461 tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga tttgcctcgt
     2521 gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc gtataagcat
     2581 cagctgctgt ctatcgcgct caacggtatg accaagttcc gcacccgcat cctgccgcag
     2641 ctgctggcag ggcagaaggc aaacggcaca cttccggcgc gccttacttt cgcattagcg
     2701 gcattgattg cgttctatcg tggtgagcgt aacggagaaa catatccggt acaggacgat
     2761 gcacactggc tggaacgtta tcagcaactc tggagccagc atcgtgacca tgttatcggt
     2821 acccaggaac tggtagcgat tgtgttggca gaaaaagacc actgggagca ggacctgacg
     2881 caagtgcctg gtctggtcga gcaggttgct aatgacctgg atgcgattct tgaaaaaggg
     2941 atgcgcgaag ctgtacgtcc gttgtgctaa taacaccgtt acttctgaac ccggttttga
     3001 ccgggtttct tatttcacta tattagttac aacatcctat gtgcctttat cattaacaga
     3061 tgccacatgc agcgcctgtt aatgttgtaa gcttgtaggt aatttgtaag tagcgtcttg
     3121 agaatgtgat cgtaatcacg tttaataggg cgactttttc acgcctgaag ataaccatga
     3181 ttgttcgtcc acaacaacac tggctgcgcc gtatttttgt ctggcacggc tcagtattat
     3241 ccaagatatc atcgcgctta ctactttgcg cataagcatt accagcgtcg cgcccattgc
     3301 cagcaggatc aggatttgcg cgctgctata aaccatggtc agcgtttgca cacttaaata
     3361 ctggcggtcg agaaaaccgg gtaatacaaa cagcagcacc accgccagca gtgccgtgat
     3421 ttcacggttg ttctgaataa acttcagcat gacgcctcct gacgctgact atcgccgaag
     3481 gcaacgcgca taatagtctc gacattaata tcgcgcccgg tcagtgcaga gtgggtaatt
     3541 tcgccctgat gcatcacata cacacgatct gccatcagtt cgatctcttc cagatcagag
     3601 gagataagca gcactgccac attctgtgcg gcgatgctgc gcaacagctg gtagatatca
     3661 ttacgggccg agacatccac gccgcgcgtc ggctcatcga caatcaatgc ttgcggcgaa
     3721 gcttccaggc atttggcaat caggattttt tgctggttgc caccggataa tgtccgtgca
     3781 gcttgttccg gttggttaaa tttaatattc agcgcccgac gataacgttc cagggtggca
     3841 ttatctttcg cggttttcgc ccagaatcca cgaaggttat gagtaagggc gcagacgttc
     3901 caggccagcg aagcatcgag attcagtccg gatgactggc gatcttccgg cagataaacc
     3961 agaccgcgca gtaaacgttc tccagtggat aatttattga tctctttacc attcagcata
     4021 atgcgtccgc cacgcaaagt acgcagacca tagagcgtct cggccagttc tgtgcgtccg
     4081 gcccccacca gcccggccag acccagaatt tctccggcat tgagcgtcag gctgacattc
     4141 ctgaaacctt cgccggtcag attttctagt gtcagcaccg acgttccggc ggcatgttgt
     4201 gggcggttgc caggtaactc cagccataat ttttggctgg cagagagcga tttttcccgt
     4261 accactggcg tgatggcctg aataatgtca tcggtagaca gttcgctggt tttgccgctt
     4321 aaggcgatgg ttccgtcgcg catcacgcta attcgatcgg caatctggcg aatttccggc
     4381 agcttatgcg agataaaaac aatacccacg ccagtagcaa gcagctcttg caagcgacga
     4441 aacaagcgtt cggtttccgc aggggtaagc gaggcggtag gttcatcgag gatcagaatc
     4501 cgcgagtcgc gcatcaggcc gcggaggatt tccaccattt ggcgatcggc ga
//
LOCUS       DAJVYM010000209         4404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVYM010000209 DAJVYM010000000
VERSION     DAJVYM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.212"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(139..3654)
                     /locus_tag="OQS60_003719"
     CDS             complement(139..3654)
                     /locus_tag="OQS60_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2389359.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OQS60_003720"
     CDS             complement(4106..>4404)
                     /locus_tag="OQS60_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS2389360.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJVYM010000210         4403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVYM010000210 DAJVYM010000000
VERSION     DAJVYM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.213"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..99)
                     /locus_tag="OQS60_003721"
     CDS             complement(<1..99)
                     /locus_tag="OQS60_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCS2389361.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="OQS60_003722"
     CDS             complement(124..1224)
                     /locus_tag="OQS60_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS2389362.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="OQS60_003723"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="OQS60_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS2389363.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="OQS60_003724"
     CDS             complement(2372..2938)
                     /locus_tag="OQS60_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS2389364.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..>4403
                     /gene="argS"
                     /locus_tag="OQS60_003725"
     CDS             3154..>4403
                     /gene="argS"
                     /locus_tag="OQS60_003725"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS2389365.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPAD"
BASE COUNT         1121 a         1128 c         1036 g         1118 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cga
//
LOCUS       DAJVYM010000211         4386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVYM010000211 DAJVYM010000000
VERSION     DAJVYM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.214"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1161
                     /gene="leuA"
                     /locus_tag="OQS60_003726"
     CDS             <1..1161
                     /gene="leuA"
                     /locus_tag="OQS60_003726"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS2389366.1"
                     /translation="FSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEFA
                     GIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNC
                     SLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSSAFAHSS
                     GIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDN
                     LYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLA
                     CGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYN
                     GRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQHNENNKETV"
     gene            1161..2252
                     /gene="leuB"
                     /locus_tag="OQS60_003727"
     CDS             1161..2252
                     /gene="leuB"
                     /locus_tag="OQS60_003727"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS2389367.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            2255..3655
                     /gene="leuC"
                     /locus_tag="OQS60_003728"
     CDS             2255..3655
                     /gene="leuC"
                     /locus_tag="OQS60_003728"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS2389368.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            3666..4271
                     /gene="leuD"
                     /locus_tag="OQS60_003729"
     CDS             3666..4271
                     /gene="leuD"
                     /locus_tag="OQS60_003729"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS2389369.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         1098 a         1180 c         1192 g          916 t
ORIGIN      
        1 ttttcttgcg aagatgccgg gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg
       61 gcgattaatg ccggtgccac caccatcaac attccggaca ccgtgggcta caccatgccg
      121 tttgagttcg ccggaatcat cagcggcctg tatgaacgcg tacctaacat cgacaaagcc
      181 attatttccg tacataccca cgacgattta ggcctggcgg tcggcaactc actggcggcg
      241 gtacatgccg gtgcacgcca ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt
      301 aactgttcgc tggaagaagt catcatggcg attaaagttc gtaaggatat tctcaacgtc
      361 cacaccgcca ttaatcacca ggagatatgg cgcaccagcc agttagttag ccagatttgt
      421 aatatgccga tcccggcaaa caaagccatt gttggcagca gcgcattcgc tcactcctcc
      481 ggtatccacc aggatggcgt gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa
      541 tctattggtc tgaaccaaat ccagctgaat ctgacctctc gttcggggcg tgcggcggtg
      601 aaacatcgca tggatgagat ggggtataaa gaaagtgaat ataatttaga caatttgtac
      661 gatgctttcc tgaagctggc ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg
      721 ctggccttca tcggtaagca gcaagaagag ccggagcatt tccgtctgga ttacttcagc
      781 gtgcagtctg gctctaacga tatcgccacc gccgccgtca aactggcctg tggcgaagaa
      841 gtcaaagcag aagccgccaa cggtaacggt ccggtcgatg ccgtctatca ggcaattaac
      901 cgcatcactg actataacgt cgaactggtg aaatacagcc tgaccgccaa aggtcacggt
      961 aaagatgcgc tgggtcaggt ggatattgtc gccaactaca acggtcgccg cttccacggc
     1021 gtcggcctgg ccaccgatat tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac
     1081 aatatctggc gtgccgcaga agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa
     1141 aacaacaagg aaaccgtgtg atgtcgaaga attaccatat tgccgtattg ccgggggacg
     1201 gtattggtcc ggaagtgatg acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct
     1261 ttgcgatgcg catcaccacc agccattacg atgtaggcgg cgcagccatt gataaccacg
     1321 ggcaaccact gccgcctgcg acggttgaag gttgtgagca agccgatgcc gtgctgtttg
     1381 gctcggtagg cggcccgaag tgggaacatt taccaccaga ccagcaacca gaacgcggcg
     1441 cgctgctgcc tctgcgtaag cgcttcaaat tattcagcaa cctgcgcccg gcaaaactgt
     1501 atcaggggct ggaagcattc tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca
     1561 tcctgtgcgt gcgcgaactg accggcggca tctatttcgg tcagccaaaa ggccgcgaag
     1621 gtagcggaca atatgaaaaa gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc
     1681 gtattgcccg catcgcgttt gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg
     1741 acaaagctaa cgtgctgcaa tcctctattt tatggcgtga aatcgtcaac gagatcgcca
     1801 cggaataccc ggatgtcgaa ctggcgcata tgtacatcga caacgccacc atgcagctga
     1861 ttaaagatcc atcacagttt gacgttctgc tgtgctccaa cctgtttggc gacattctgt
     1921 ctgacgagtg cgcaatgatc actggctcga tggggatgtt gccttccgcc agcctgaacg
     1981 agcaaggttt tggtctgtat gaaccggcag gcggctcagc accagatatc gcaggcaaaa
     2041 acatcgccaa cccgattgcg caaattctgt cgctggcact gctgctgcgc tacagcctgg
     2101 atgccgatga tgcggcttgc gccattgaac gcgccattaa ccgcgcatta gaagaaggca
     2161 ttcgcaccgg ggatttagcc cgtggcgctg ccgccgttag taccgatgaa atgggcgata
     2221 tcattgcccg ctatgtggca gaaggggtgt aatcatggct aaaacgttat acgaaaaatt
     2281 attcgacgct cacgtagtgt acgaagccga aaacgaaacc ccgctgttat atatcgaccg
     2341 ccacctggtg catgaagtga cctcaccgca ggcgttcaat ggtctgcgcg cccacggtcg
     2401 cccggtacgt cagccgggca aaaccttcgc cactatggat cacaacgtct ctacccagac
     2461 gaaagacatt aatgcctgtg gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa
     2521 ctgcaaagaa tttggcgtcg aactgtatga cctgaatcac ccgtatcagg ggatcgtcca
     2581 cgtaatgggg ccggaacagg gcgtcacctt gccggggatg accattgtct gcggcgactc
     2641 gcataccgcc acccacggcg cgtttggcgc actggccttt ggtatcggca cttccgaagt
     2701 tgaacacgta ctggcaacgc aaaccctgaa acagggccgc gcaaaaacca tgaaaattga
     2761 agtccagggc aaagccgcgc cgggcattac cgcaaaagat atcgtgctgg caattatcgg
     2821 taaaaccggt agcgcaggcg gcaccgggca tgtggtggag ttttgcggcg aagcaatccg
     2881 tgatttaagc atggaaggtc gtatgaccct gtgcaatatg gcaatcgaaa tgggcgcaaa
     2941 aaccggtctg gttgcaccgg acgaaaccac ctttaactat gtcaaaggcc gtttgcatgc
     3001 gccgaaaggc aaagatttcg acgacgccgt tgcctactgg aaaaccctgc aaaccgacga
     3061 aggcgcaact ttcgataccg ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac
     3121 ctggggcacc aatccaggcc aggtgatttc cgtgaacgac aatattcccg atccggcttc
     3181 gtttgccgat ccggttaaac gcgcgtcggc agaaaaagcg ctggcctata tggggctgaa
     3241 accgggcatt ccgctgaccg aagtggctat cgacaaagtg tttatcggtt cctgtaccaa
     3301 ctcgcgcatt gaagatttac gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc
     3361 aggcgtgcag gcactggtgg ttcccggctc tggcccggta aaagcccagg cggaagcgga
     3421 aggtctggat aaaatcttta ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat
     3481 gtgtctggcg atgaacaacg accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa
     3541 ccgtaacttt gaaggccgcc aggggcgcgg cgggcgcacg catctggtca gcccggcaat
     3601 ggctgccgct gctgctgtga ccggacattt cgccgacatt cgcaacatta aataaggagc
     3661 acaccatggc agagaaattt atcaaacaca caggcctggt ggttccgctg gatgccgcca
     3721 atgtcgatac cgatgcaatc atcccgaaac agtttttgca gaaagtgacc cgtacgggtt
     3781 ttggcgcgca tctgtttaac gactggcgtt ttctcgatga aaaaggccaa cagccaaacc
     3841 cggacttcgt gctgaacttc ccgcagtatc agggcgcttc cattttgctg gcacgagaaa
     3901 acttcggctg tggctcctcg cgtgagcacg cgccctgggc attgaccgac tacggcttta
     3961 aagtggtgat tgcgccgagt tttgctgaca tcttctacgg caatagcttt aacaaccagc
     4021 tgctgccggt gaaattaagc gatgcagaag tggacgaact gtttgcgctg gtgaaagcca
     4081 atccggggat ccatttcgac gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa
     4141 cctatcgctt taccatcgat gccttccgcc gccactgcat gatgaacggt ctggacagta
     4201 ttgggcttac cttgcagcac gacgacgcca ttgccgctta tgaagcaaaa caacctgcgt
     4261 ttatgaatta atccccttgc ccggtcaaat gaccgggctt tccgctatcg tccacgtcat
     4321 caaacgtctt taacctttgc ggttaaaaat aatgcgacaa cagaaataac cgcaattacc
     4381 cagtag
//
LOCUS       DAJVYM010000212         4325 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVYM010000212 DAJVYM010000000
VERSION     DAJVYM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.215"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..579)
                     /locus_tag="OQS60_003730"
     CDS             complement(<1..579)
                     /locus_tag="OQS60_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2389370.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OQS60_003731"
     CDS             complement(642..1091)
                     /locus_tag="OQS60_003731"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS2389371.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OQS60_003732"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OQS60_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1090 c         1167 g         1041 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactg
//
LOCUS       DAJVYM010000213         4323 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVYM010000213 DAJVYM010000000
VERSION     DAJVYM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.216"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            387..1469
                     /locus_tag="OQS60_003733"
     CDS             387..1469
                     /locus_tag="OQS60_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS2389372.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OQS60_003734"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OQS60_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCS2389373.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="OQS60_003735"
     CDS             complement(2127..2267)
                     /locus_tag="OQS60_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCS2389374.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OQS60_003736"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OQS60_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCS2389375.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="OQS60_003737"
     CDS             complement(2623..2913)
                     /locus_tag="OQS60_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS2389376.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="OQS60_003738"
     CDS             complement(2906..3076)
                     /locus_tag="OQS60_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389377.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="OQS60_003739"
     CDS             complement(3076..3531)
                     /locus_tag="OQS60_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCS2389378.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="OQS60_003740"
     CDS             3796..4173
                     /locus_tag="OQS60_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389379.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1232 a          976 c          929 g         1186 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggt
//
LOCUS       DAJVYM010000214         4314 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVYM010000214 DAJVYM010000000
VERSION     DAJVYM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.217"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1871)
                     /gene="speC"
                     /locus_tag="OQS60_003741"
     CDS             complement(<1..1871)
                     /gene="speC"
                     /locus_tag="OQS60_003741"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS2389380.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            2273..2980
                     /locus_tag="OQS60_003742"
     CDS             2273..2980
                     /locus_tag="OQS60_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS2389381.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3086..3161
                     /locus_tag="OQS60_003743"
     tRNA            3086..3161
                     /locus_tag="OQS60_003743"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3119..3121,aa:Phe,seq:gaa)
     gene            3359..>4314
                     /locus_tag="OQS60_003744"
     CDS             3359..>4314
                     /locus_tag="OQS60_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS2389382.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1115 a         1142 c         1039 g         1018 t
ORIGIN      
        1 tgcgcatcct gggggttcat caccactgac gggaaactct gctggcggaa catcgctttt
       61 tgtaggtctt tgacgtcgaa actgacatac agatcgtgca tctcctgaca caactggcgc
      121 agggtgtagt cgcgatagcg caccggatat ttgttataaa cgctcggcaa cacctcagcc
      181 agcggcgagt catcctcaat atgctgttca aattgcgcca gcatcgccac cagttgtgcc
      241 agcttctcgt ggctttccgc cggagttaat aagaacagaa tggagttgag atcgcacttc
      301 tccggcacaa tgccgttctc acgcagatag tgcgccagaa tcgtcgccgg aacgccaaag
      361 tcgctatatt cgccggtttc ggcattgata cctggtgtag tgagtaacag cttgcacgga
      421 tcaacaaaat actgatccgc ggcatatcct tcaaagccgt gccacttcgc ccccggctca
      481 aaactgaaaa aacggcggtc gctggctaac acagatgtcg gataatcctg ccacaatttg
      541 ccatcaacaa cgggcgggat aaacgggcgg aacagcttac agcgcgcaag aatagccttg
      601 cgcgactcaa tacccaactc aacgcactca gcccacagcc gacgcccact ctccccttca
      661 tgaattttgg cgttaacatc cagtgcagca aacagcggat agaaagggct ggtagaagca
      721 tggagcataa aggcgttatt caaccgctta tgcgggcaaa aacgcgcctg tccgcggata
      781 tggttatctt ttttatggat ctgcgacgtc tgtgagaatc ccgcctgctg tttgtgcacc
      841 gactgagtca caaagatccc cggatcgttt tcgttaagtt ctaatagcag cggcgagcta
      901 tccgccatca tcgggataaa ctgttcgtaa ccgacccacg cggaatcaaa cagaatgtaa
      961 tcacacagat gcccaacggt atcgatcacc tgacgggcgt tatagacagt tccgtcatag
     1021 gttcccagct gaataatcgc caggcgaaac gggcgcggca gctcggcttt ttctggcgca
     1081 acgtcgcgaa tttgctggcg cagatactct tcattaaaac agtgcgcatc aataccgcca
     1141 atgaaaccaa acgggttgcg tgaagcttcc agatagaccg acgtcgcccc cgcctgaatc
     1201 agcgcgccgt gatgattcga cttatggttg ttacggtcga agagcaccag atcgccacgc
     1261 gttaacagcg cattcgtcac cactttattc gctgccgatg tgccgttcag cacaaaatag
     1321 gttttatcgg catgaaagac tttggctgcg aatttctgcg catccttcgc cgatccttca
     1381 tgaataagca gatcgcccaa ttttacgtca gcgttacaca tatcggcgcg aaagacgttc
     1441 tcaccaaaga aatcgtaaaa atggcgtccg gcaggatgct ttttaaaaaa cgcaccatgt
     1501 tgatgtccag ggcaagcaaa ggtgctgttg cccatctcaa cgtactgcgt cagcgtgtca
     1561 taaaacggtg gcagcaaatt ctcttcatac tgacaggctg cggattccag ctccagccac
     1621 tgctgctcgt tgccgttgat taccgccgta acgcccgcag gtaattcaac agcatgttcg
     1681 gaatacaaaa acaccggtag atgaaaaccg gtgcgcttaa gcaacgcaag aatgccactg
     1741 cgactatccg cagcggtaat gacgactgcc gcgacatccg taaaatcagt atctcccaac
     1801 gccaccacgc ggcgatgagt agaaagtcgg gataccagtt cactactggc ggcaatattc
     1861 attgatttca taagcgcaaa acccgtttcg gggaagtaag aataccggac aaagtgtaaa
     1921 accctgcccc atgagatatg ggtcaaactg gtcaccagct ccgacgaccg ccagacatca
     1981 gtaaaagcag aaacgctctg attttactgt tgtcctgcag tgaacgtgcg ttaacttcac
     2041 cgcatgagca gtaacataga aagggaaacg tttcgcgcga aacggcgata agcgagagaa
     2101 tgtaaggaga tggcgtgcat cgggcaaact ccgtacagag aggagaaaat tcgcgcaatc
     2161 atggcacctt tcgctaaggc gtgcaagcca ggactttgcg accagacaag ccatcaacaa
     2221 ggcaaaaagt tcataataag aaaatcaaac aatacagcta acaggaactc ttgtggtcat
     2281 cggccctttt atcaacgcta gtgcagtctt actgggtggc gttctcggcg cactgctcag
     2341 ccaacgctta ccggaacgta tccgcgtctc catgacatct atttttggtc tggcatcgct
     2401 ggggattggt attttactgg ttgtgaaatg cgccaacctt ccagcgatgg ttttagctac
     2461 gctacttggc gctctaattg gcgaaatttg tttgctggag aaaggtgtca atacagcagt
     2521 cgccaaagca caaaatctgt ttcgtcactc acgtaagaag ccagcgcatg aatcttttat
     2581 tcagaattat gtcgcgatta ttgtcctgtt ttgcgccagc ggcaccggga tcttcggggc
     2641 gatgaacgaa gggatgaccg gcgatccgag tattttaatc gccaagtcat ttcttgattt
     2701 ctttacggcg atgatcttcg cctgctcgct gggtattgcg gtgtcggtaa ttagtatccc
     2761 attactgatc atccagctaa cgctagcgtg ggctgccgcg ctgatattac cgctgaccac
     2821 accgtcgatg atggcagact tcagcgccgt aggcggttta ttgctgctgg caaccggatt
     2881 acgcatctgt ggcattaaaa tgttcccggt ggtcaacatg cttccagcac tccttctggc
     2941 aatgccgctt tccgccgcct ggaccgcctg gtttgcctga caatgcgtgc aatagcggca
     3001 aagtgatgat agattgtgca gtctgcagta aattgaagaa atttgattga cgagacgagg
     3061 cgaatcaggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc aggggattga
     3121 aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat caacggacct
     3181 ccacggaggt ccgtttttcg tttcagaacg ccacgattta agcgttctgc ctacaaatca
     3241 attctaccga actcaaccag attcccccca catcaacccc attgtgtggg tataattgcg
     3301 ggcatacccc agttcgacag aatttgtacc ctctttaacg cattcagaag gctgaactat
     3361 ggcactgact gacgcaaaaa tccgggctgc aaagcccact gacaaggctt ataaactcac
     3421 tgacggggct ggcatgttcc tgctggtaca tcctaatggc tcccgttact ggcgtctccg
     3481 ttatcgtatt ctgggtaagg agaagactct ggcacttggt gtgtatccag aagtttctct
     3541 ctccgaagct cgtacaaaac gggatgaggc ccgaaaactg atttcggagg gggttgaccc
     3601 ttgcgaacag aaaagagcta aaaaagtagt ccctgattta cagctctctt ttgaacatat
     3661 tgcgcgacgc tggcatgcca gtaataaaca atgggcacaa tcacacagca ataaagtact
     3721 caaaagcctt gagactcacg ttttcccctt tatcggcaac cgggatatca caacactcaa
     3781 taccccggat ctgcttatcc ctgttcgtgc tgcagaagct aaacaaattt atgaaatcgc
     3841 cagtcgtctg cagcaaagaa tatctgccgt aatgcgttat gccgtacagt ctggcatcat
     3901 cagatataat cctgctctgg atatggctgg cgcattgact acggtaaaac gccagcatcg
     3961 ccccgctctt gatctttctc gcctgcctga acttttgtcg cgtattggca gttacaaagg
     4021 gcaacctgtc acccagcttg ccgttatgct gaatttactg gtttttattc gttccagtga
     4081 actcagatac gcccgctggt ctgaaattga tattgacaat gccatgtgga ctattccagc
     4141 cgaacgcgaa cccctgccag gcgtaaaatt ctcacaccgg ggctccaaga tgcgaacacc
     4201 acatcttgtg ccactcagca aacaggctgt agccatactg acagaactac agacatgggc
     4261 tggtgaaaat ggtctgatct ttacgggagc acatgacccg cgtaaaccaa tcag
//
LOCUS       DAJVYM010000215         4262 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVYM010000215 DAJVYM010000000
VERSION     DAJVYM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.218"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            288..896
                     /locus_tag="OQS60_003745"
     CDS             288..896
                     /locus_tag="OQS60_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS2389383.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="OQS60_003746"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="OQS60_003746"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS2389384.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..4226
                     /gene="selB"
                     /locus_tag="OQS60_003747"
     CDS             2382..4226
                     /gene="selB"
                     /locus_tag="OQS60_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS2389385.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          964 a         1078 c         1241 g          979 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgctggttt gattcgccga
      481 tcatcgctga atatattgag ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cgctggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgct gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg acagccagac gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat
     1381 cgcgatcggg cgctggcgca actgctgtgc cgtattacgg gggcggaaga tgcctgtatc
     1441 gtcaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtagtat ctcgcggcga attggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattatcgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agcttctccg gcgacaagtt gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ctccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccgatgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggtgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct agttttatcg acgtacccgg tcatgaaaag tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gcgtgatggc gcagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac
     2761 gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttaccaggac
     2881 gtgaacacgc cagccaacat agtgtccgcc tcgcgatcga ccgcgcattt accgtaaaag
     2941 gtgccggtct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc
     3061 caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt atttccggaa aaataaggaa atgattaatt aatttttaaa ataaattaat
     4261 ac
//
LOCUS       DAJVYM010000216         4243 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVYM010000216 DAJVYM010000000
VERSION     DAJVYM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.219"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(7..1068)
                     /locus_tag="OQS60_003748"
     CDS             complement(7..1068)
                     /locus_tag="OQS60_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS2389386.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="OQS60_003749"
     CDS             complement(1065..2462)
                     /locus_tag="OQS60_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS2389387.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="OQS60_003750"
     CDS             2617..3615
                     /locus_tag="OQS60_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2389388.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4243)
                     /gene="ompG"
                     /locus_tag="OQS60_003751"
     CDS             complement(3726..>4243)
                     /gene="ompG"
                     /locus_tag="OQS60_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCS2389389.1"
                     /translation="QRWKIAPDWDVKLTDDLRFNGWLSMYKFANDLNTTGYADTRVET
                     ETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTR
                     IGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKF
                     HYAGVGVNYSF"
BASE COUNT         1031 a         1157 c         1047 g         1008 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gca
//
LOCUS       DAJVYM010000217         4199 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVYM010000217 DAJVYM010000000
VERSION     DAJVYM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.220"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1361)
                     /locus_tag="OQS60_003752"
     CDS             complement(<1..1361)
                     /locus_tag="OQS60_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS2389390.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="OQS60_003753"
     CDS             complement(1426..1545)
                     /locus_tag="OQS60_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS2389391.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="OQS60_003754"
     CDS             complement(1700..1918)
                     /locus_tag="OQS60_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389392.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="OQS60_003755"
     CDS             complement(2272..3750)
                     /locus_tag="OQS60_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCS2389393.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1185 a          919 c          739 g         1356 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtga
//
LOCUS       DAJVYM010000218         4098 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVYM010000218 DAJVYM010000000
VERSION     DAJVYM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.221"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..2074
                     /gene="ybiO"
                     /locus_tag="OQS60_003756"
     CDS             <1..2074
                     /gene="ybiO"
                     /locus_tag="OQS60_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS2389394.1"
                     /translation="LADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTV
                     LQKVTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYW
                     LIRLCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDN
                     LNAGSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLS
                     WLSSLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLN
                     FAEHSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIG
                     IAAFVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLL
                     SAWGLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPL
                     PSARTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLV
                     KDIITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTF
                     ANFVRGIGSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGL
                     SNTAFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEP
                     LPPGEPTL"
     gene            complement(2071..2997)
                     /gene="rlmF"
                     /locus_tag="OQS60_003757"
     CDS             complement(2071..2997)
                     /gene="rlmF"
                     /locus_tag="OQS60_003757"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS2389395.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3273..3533
                     /locus_tag="OQS60_003758"
     CDS             3273..3533
                     /locus_tag="OQS60_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2389396.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            3798..>4098
                     /locus_tag="OQS60_003759"
     CDS             3798..>4098
                     /locus_tag="OQS60_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS2389397.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT          952 a         1073 c         1035 g         1038 t
ORIGIN      
        1 actggcggat gtgctggata acgacacctc gcgtaaagaa ttgatcgacc agttgcgtac
       61 tgtcgccgcg acgccccctg cggaaccggt cccgaagatc gtgccgccga cgctggttga
      121 agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc cattatggtg aagccctttc
      181 cgcccgcttc gggcaacttt atcgcaatat caccggctcc ccgcataagc catttaatcc
      241 acaaaccttc agcaatgcgc tgagccattt ttcaatgtta gcggtattag tgtttggttt
      301 ttactggctg attcgcctgt gcgcactgcc gctgtatcgc aaaatgggtc agtgggcgag
      361 gcaaaaaaat cgtgagcgca gcaactggtt gcagcttccg gcgatgatta tcggggcgtt
      421 tattatcgac ctgctgttac tggccctgac attatttgtc ggccaggtat taagcgacaa
      481 cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc
      541 cctgattgaa tttttcaaag ccgtactacg cctgattttt tgcccaaacg tggcggagct
      601 gcgcccgttc acgattcagg acgagagcgc ccgttactgg agtcgtcgcc tgagctggtt
      661 aagcagtttg ataggttatg gcctgattgt ggccgtgccg attatctcta atcaggtgaa
      721 tgtacagata ggtgcgctgg cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt
      781 gtacctgatc tttcgtaata aaaaagagat tacccagcat ttgctcaact ttgcggagca
      841 ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg ctggtgtggc actggctggc
      901 aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc gatccgggca acagcctgaa
      961 atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc
     1021 cggtatgttt tcccgctggc tggcaaaaac catcactctc tcgccacata ctcagcgtaa
     1081 ctatccagag ttgcaaaaac ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg
     1141 tattctgacc gtctgcgtgg cggtaatgtt gctgttgagc gcatggggat tgttcgattt
     1201 ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta gatatcctga tccgtatcgc
     1261 acttattctt tttttctcgg cggttggctg gacggtgctc gccagtttga tcgaaaaccg
     1321 gctggcttcg gatattcatg gccgcccgct acccagcgcc cgtacgcgta ccctgctgac
     1381 gctgttccgt aacgcgctgg cggtgattat cagtaccatc accatcatga ttgtgttgtc
     1441 ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc ggggcattag gtctggctat
     1501 ctcgtttggt tcgcaaacgc tggtgaaaga tatcattacc ggggtgttta ttcagtttga
     1561 aaacggcatg aacactggag atttggtgac tatcgggccg ttgaccggca cagtggaacg
     1621 gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg
     1681 gtcttcgata accacctttg ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta
     1741 cgatgttgat cgccatgaag atgctgataa agccaatcag gcactgaaag atgcggtagc
     1801 ggaattaatg gaaaacgaag aaattcgcgg gctgattatt ggtgaaccga attttgccgg
     1861 gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt tcgttcacca cgctgccact
     1921 caaacagtgg acggtacgct ttgccctcga cagccaggtg aaaaaacatt tcgacctggc
     1981 gggcgttcgc gcgccagtgc agacttatca ggtgctgcct actccgggcg cgaccccggc
     2041 tgaaccgtta ccgccggggg agccaacgct ttaacgctgg cgattgacaa aacggcggcg
     2101 ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc tgcttttgcc cctgggccat
     2161 ctcttttttt accaccttca ccgcgcccac gtcggtcaga gcacgataca acggcggtaa
     2221 gttttcacca cgagatacca gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct
     2281 ctcttcaatc atctttttga taaaggcgac ttcaccacct tcacaccaca actcctgttg
     2341 ctgaccacca aagttcagtg catcgtcttt gttcagcccc aggttacggc gtttacgctc
     2401 actacctgcc cgcgctgcag cggcggaatc gtggaatggc gggttacaca aggtcgcgtc
     2461 gtattgctcg tttttatgga tgatgccgtt aaaaattgcc ccactctctt tttgccgacg
     2521 cagacgaatg gcgcggttaa gccccggatt agcactgata atcgcctgcg cactgcttaa
     2581 cgcctggctg ctggtttcac taccggtaaa acgccagcca tattcatgta cgccaatcag
     2641 cggataaata cagttcgcac caacgccgat atccagaatg ctggcattcg ctggaattgt
     2701 tccgcttgct tcagccagta aatcggcaag gtgatgaata taatccgccc ggcccggtac
     2761 tggtgggcag agaaaaccgt cggggatatc ccagttcgct acggcgtaaa aatgggccag
     2821 caacgcctta ttgagcgcct tcaccgccag cggattggca aagtctacgc tttgctcccc
     2881 ggcgggtgta agcgtgagga attgcctgag ttcaggattg acctgacaaa gcgtggcgag
     2941 atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc ggtttctggg cggacatagc
     3001 attctccttt aagcagcggc gtaagatacc cgcataacgc gacagggtaa atatctggct
     3061 gcgtttatca ttccccaaaa ccttaacatg gctatggttt atggcaattc atcataaaaa
     3121 tcagcattat gcatgtcatt aatttgtctt attttgtaaa aatatatcgt tcaaaaaata
     3181 agcacatctc tgtcaatttt ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag
     3241 gcactacccg ttgtatttat gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg
     3301 ccaccgtcct gagcggaatc tctctcacgg cttatgccgc gcaaccgatg agtaacctgg
     3361 acagcggtca gctacggccc gccggtaccg tttcggcaac tggcgcatca aacctaagcg
     3421 atctggagga taaactggca gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta
     3481 attccgcagg cggaaatgac cagatgttcg gtactgcaac catctacaaa taaccccacc
     3541 aacgtaactg cattcgcctc tggatgcagt tttcgcctgt tttgtaatca aaaaacatca
     3601 aatatgaact caatgtaaat aaatgtattt ctttttcgcg caatgggtga tagaaaatcg
     3661 cgccaagtga taatgcttat caaaattatt atcacttgca tgaacactat cacgggatta
     3721 acagtggcat cgcatccgca gagatgcttt ctcgtggcag tgaaaatttc aacatataag
     3781 aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa tttcattcgc
     3841 tcacgttctt tgccggtctt tgtattggca tcacgcctgt ggctcaggca ctcgccgccg
     3901 aagggcaaac taacgcggat gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg
     3961 cgccacaaca atctgccgat ccgaaattct cgcgtccggt agcggatact acccgcacga
     4021 tgacggtgat ttctgaacaa gtgattaaag atcagggcgc aaccaacctt accgacgcgc
     4081 tcaaaaacgc ccccggcg
//
LOCUS       DAJVYM010000219         4089 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVYM010000219 DAJVYM010000000
VERSION     DAJVYM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.222"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="OQS60_003760"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="OQS60_003760"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS2389398.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="OQS60_003761"
     CDS             932..1384
                     /locus_tag="OQS60_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS2389399.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="OQS60_003762"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="OQS60_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="OQS60_003763"
     CDS             complement(2816..3109)
                     /locus_tag="OQS60_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389400.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>4089
                     /gene="nlpA"
                     /locus_tag="OQS60_003764"
     CDS             3331..>4089
                     /gene="nlpA"
                     /locus_tag="OQS60_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCS2389401.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          901 a          907 c         1076 g         1205 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcgct gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat
     3841 ataaccgata atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc
     3901 gtactggatg atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca
     3961 gggctttctc cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat
     4021 attttggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt tctgcaatct
     4081 tatcaatca
//
LOCUS       DAJVYM010000220         4076 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVYM010000220 DAJVYM010000000
VERSION     DAJVYM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.223"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..784
                     /locus_tag="OQS60_003765"
     CDS             <1..784
                     /locus_tag="OQS60_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2389402.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="OQS60_003766"
                     /pseudo
     CDS             797..1757
                     /locus_tag="OQS60_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OQS60_003767"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OQS60_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS2389403.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OQS60_003768"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OQS60_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS2389404.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAJVYM010000221         4059 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVYM010000221 DAJVYM010000000
VERSION     DAJVYM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.224"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            35..1181
                     /locus_tag="OQS60_003769"
                     /pseudo
     CDS             35..1181
                     /locus_tag="OQS60_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1475..2716
                     /gene="agp"
                     /locus_tag="OQS60_003770"
     CDS             1475..2716
                     /gene="agp"
                     /locus_tag="OQS60_003770"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS2389405.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2754..2981)
                     /locus_tag="OQS60_003771"
     CDS             complement(2754..2981)
                     /locus_tag="OQS60_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS2389406.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="OQS60_003772"
     CDS             complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="OQS60_003772"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS2389407.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3659..>4059
                     /locus_tag="OQS60_003773"
     CDS             3659..>4059
                     /locus_tag="OQS60_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389408.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         1145 a          865 c          954 g         1095 t
ORIGIN      
        1 cgtcattatt tattaaattt tatagacgct atatatgggt agtaatatac atggaattag
       61 ttgcactgca aataattatt tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa
      121 aaatcaaaca tattcaatca cgctttttga aaacacactg gtgtgtttta tgcggttata
      181 caatgaactc agacgtaaag taaatgaaga ggatactcca tgtctggaat gtgaatcact
      241 agaaaaagaa tttgaggaaa tgcagaatga taatgatcta tcattattta tgagaacatt
      301 gcgtactaat gatacacaaa tttattcagg ggtttcagga ggtattacat atactataca
      361 atatgttcaa aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat
      421 cacagatttt aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc
      481 gtgtgatgat attttttctg agtattgttt agatgatgaa aatatgagta tccagccaga
      541 acagataaat acgccgggaa tatctgattt agatactggt tttaaaagaa catgagtatt
      601 catgggcgct gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg
      661 ttgataaaca aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg
      721 taaagacgtt atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt
      781 ttttatcatg gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca
      841 aaaaatgcat actgcattta cccgaattta tatttaatga taacaaggca cgccttgttt
      901 tagacaccga cccagagcag aaggttaatg aggtgaaaaa tttcctcact gctctttcag
      961 atagcatttg ctcattattt attgtttaca gcaatgtggc atcaatctcc ttagggaatg
     1021 aatccttttc aacagatgac gaccttgagt atgggtattt aataaacact ggcaatcatt
     1081 atgacgttta cctccctcct gaactttttg ctcaggctta cgagttaaac aataaggaaa
     1141 ggaatgcgca aattgatttt ttaactcgtt atgcaattta atgacaaagg catgtgctaa
     1201 aaaccattgt tattagcctc acttctctta ttattggtac tattgcctct gtattagtaa
     1261 cgccgctgat attctgttta tccacaggtg caattatcag cggcgtacgc gaggcagggg
     1321 ctaatcaggc atagtttgcg tcaaaccttg cctgtttttg aagatgtata tagaaaaaca
     1381 ggcgttcaac aagccatttt gcgaacctgt tcccggaaaa aagtcatatt tctgtcacac
     1441 tctttaatga ttgataacaa aagaggtgcc aggaatgaac aaaacgctaa tcgccgcaac
     1501 tgtggcaggg atagttttac tcgcttcaaa tgctcaggca caaaccgtac cggaaggcta
     1561 tcagctacag caagtgctca tgatgagccg ccataactta cgtgcgccgc tggcgaacaa
     1621 tggcagtgtg ctggagcagt cgacgccgaa taaatggcca gaatgggacg tccccggtgg
     1681 gcaactcacc accaaaggtg gcgtgctcga agtgtatatg ggccattaca tgcgtgaatg
     1741 gctggcagag caggggatgg tgaaatcggg ggaatgcccg ccgccggaca ccgtttatgc
     1801 ctatgccaat agtctgcaac gtaccgttgc gaccgcacag ttctttatta ccggcgcatt
     1861 cccggggtgt gatattcctg tgcatcacca ggaaaaaatg ggcactatgg acccaacctt
     1921 taacccggtg atcaccgatg attccgccgc attcagtgaa caggcggtgg cggcaatgga
     1981 gaaagagctc agcaaactcc agcttaccga cagctaccag ctactggaaa aaatcgttaa
     2041 ctataaagat tcccctgcct gtaaagagaa acaacagtgt tcgctggtgg atggcaaaaa
     2101 tacctttagc gccaagtatc aacaagaacc aggtgtttcc gggccgctga aagtcggcaa
     2161 ctcgctggta gatgcgttta ctttgcaata ttacgaaggt tttccgatgg atcaggtggc
     2221 ctggggagaa atcaaatctg accagcagtg gaaggtgttg tcgaagctta aaaacggcta
     2281 ccaggacagc ctgtttacct caccggaagt ggcgcgcaat gttgcgaaac cgctggtcag
     2341 ttatatcgac aaagctctgg tcaccgatcg caccagcgca ccgaaaatta cagtgttggt
     2401 tgggcacgac tccaacattg cctctctgtt aacggcgctg gatttcaaac cgtatcagtt
     2461 gcatgaccag aacgaacgca cgccgattgg cggcaaaatc gttttccagc gttggcatga
     2521 cagcaaagcc aatcgcgatt tgatgaaaat tgaatatgtg tatcagagtg cggaacagtt
     2581 acgtaatgcc gatgcgttaa ccctgcaggc acctgctcag cgtgtgacgc tggaattaag
     2641 cggttgcccg atagatgcca acggtttctg cccgatggat aaatttgata gcgtgttgaa
     2701 tgaagcggtg aaataacaga aaactccccc gcgagaagcg ggggagtcgc tggttaaacg
     2761 tttttacgtt cgatggtctg ttcgccccaa aaaagcgaat ctttatcggc cttagcaaag
     2821 gctttgacta acacttcatc gctaccttct tcccaaatct tttctgccat tttttcgtcg
     2881 tacccggcga cttcgaaaat ggcctcggct atttccggcg acgtattgcg cagagatgcc
     2941 cattcaccga cgtgatgagc tttcgcttct tgagttggca tgcgtatcct cctgttgaag
     3001 attagccgtt aagtttaact gccagacctg cgacatattc cccttgataa cgagcaatag
     3061 acagttcttc ctggcttggc tggcgtgagc cgtcaccgcc tgcgatggtg gttgcgccgt
     3121 acggcgtacc gccgcgaacc tgtgaaacgt caaataattc ctgcgctgcg tagccaatgg
     3181 ggacaatcac catgccgtga tgcgcaaggg tcgtccaggt tgaagtaata gtttgttcct
     3241 gaccgccgcc agtaccggtg gaactaaaga cgctcgccag ttttccgtat agtgcgccgg
     3301 aagcccacag gccgcccgtc tggtcgagga aggtacgcat ttgaccggac atgttgccaa
     3361 agcgggtagg tgtaccaaaa ataatggcgt cgtaatcggc cagttcttgc ggggttgcaa
     3421 ccggtgcagt ttgcgtttta ccgcctgctt tttcaaataa ttgcggcggc atggtttccg
     3481 gtacacgctt aacgacaact tctgcgccat cgactttgct tgcaccctca gcgactgcgc
     3541 gtgccatcgt ttcaatatgt ccgtacatgg aataataaag cactagtatt cttgccttaa
     3601 cactcatatc attgttctcc tgttcaatga accacttaga taagcatagt ccgaagatat
     3661 gaagctgcta gtgacataca tcaggtggag tactaaagag caagactcag gagactcctt
     3721 aagacggcaa acaaacctca ttgatgcttt ctactctaag cacaaaaacg attattacct
     3781 tcttcctgct cacagatatg ttgataaggg taaatctggt tttcaccagc aacataagaa
     3841 tcaaggttct gacttcagac gtatgtttga aaatgttatg tctggggcta ttccagaagg
     3901 ttccttgatt gttgtagaga actttgaccg cttcagtagg gctgatatcg acacagctat
     3961 agatgatgtc aggcagatac tgaggaaggg ggtctctata ctgactctgg gtgatggtga
     4021 actctacgac aagtcagcct taacagaccc tgtgaagtt
//
LOCUS       DAJVYM010000222         4058 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVYM010000222 DAJVYM010000000
VERSION     DAJVYM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.225"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            334..1223
                     /locus_tag="OQS60_003774"
                     /pseudo
     CDS             334..1223
                     /locus_tag="OQS60_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1223..1513
                     /locus_tag="OQS60_003775"
     CDS             1223..1513
                     /locus_tag="OQS60_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389409.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1834..2124
                     /locus_tag="OQS60_003776"
     CDS             1834..2124
                     /locus_tag="OQS60_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389410.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2124..2543
                     /locus_tag="OQS60_003777"
     CDS             2124..2543
                     /locus_tag="OQS60_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389411.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            2711..3034
                     /locus_tag="OQS60_003778"
     CDS             2711..3034
                     /locus_tag="OQS60_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389412.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3464..>4058
                     /locus_tag="OQS60_003779"
     CDS             3464..>4058
                     /locus_tag="OQS60_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389413.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1349 a          717 c          838 g         1154 t
ORIGIN      
        1 aggtgattca gatgttaaga agtatccctg tgaacaataa atgaccaatc agtaatcatt
       61 ctgtgaaata attgttgcaa tttataatcg catctgttat agtttctata tgggctgata
      121 aaaaagttca tgtcttgaag tggtaacact tggggaggtg tcagatggtt ggcgtatatt
      181 aacctctcat tcagggtata cctcagcgtt ttaatcatcg tactgtttca ggcatggact
      241 tctttatcag tccatcaatc ccccagatat tttaatcaca caggctaatc ggttttatcc
      301 ggttggcctt ttcacgtatg aggtaattta tgaatgtcta aatataaccc aaatcagttt
      361 tataaatttg atgtgcgaat ctctcaactt tcagaagaaa ttaacgcagc agcaagattg
      421 atttatatgg tcatggtcca tagctgtgac ttctatgaag agaaatacaa atcatattca
      481 ccaagtattg accatctcgc ttatatggct ggtgtatcaa agagaacagc aatcagagcc
      541 attgagacac ttgagaaaca cggtttaatt caggtgcaaa agaatcaggg gcgtcaaagc
      601 acttatcgtg tattcacact ggatgataag ccggaattac ttgtaagacc tacatcacag
      661 gaagttgcag cctcagtaag acaaaatgac accactacca gtgacaaaat gtcatggggg
      721 tagtgacaaa atgtcacccg tgttagagtt tagataagtt attagataaa acattagata
      781 ataatattac agataagaat aaagagactg gacttaatga tgatattaaa gaagatgact
      841 ttctttcttt ggatgaatta aatgattttc tctctatgaa tgaatctgac cttgaagtaa
      901 gctctaaaaa gacttctaaa caagacagag aaatcactga tgtgagtgtt gatagtgacc
      961 ataaaaacac acagtgcccc gccactgatg atgagttgga ccccttcgat gatgaagagg
     1021 atgaacgtga aaaagccatt aagagggcag agaagaaaaa gagattcgag cttgcaagac
     1081 aaaagaacaa agtatcgggt ttagacccat tcgatgacag tgaagatgat tattatcttt
     1141 caccgaaaaa gaaacaaact caggatgaca agaagcaaaa aattaaaccg aagatgtatt
     1201 ttgccggtgg aggaattaag taatgaaaga atatcacggt gaaaagagat acaaagatta
     1261 cctgttaaag cgttacagca tttctcgtga aggtcatctt atgaaggata ctcacgggga
     1321 agtttaccgg attagaccaa agaaagaagg gaggggttat ttcttttacg atggtgtgac
     1381 agatttaaag attgacgctt tgaggtttgc agtgctgtat cattttgata tctggaattc
     1441 aatccaccag ctacgattaa aagacggtga ccccagcaac ttaaaggcca ccaatattat
     1501 cacaaaacgt tagtttgttt taccatcatc aatcgtaatt gtgatttcat atttgaaaag
     1561 cttaatgctg aaattgaatc gtgtacttct ggcgataatc atttttaaaa gctgatacac
     1621 aagaagcacc agaaacacaa agcaacctaa gcttgcgaat ctgtgagatt cagcaattgt
     1681 ttcaagaaag cccattaatt ccccttattt atcaaaggga atgacctgta tcagccccct
     1741 acaaccatat taacatgtaa atgagttgtt gacaatatgt tataaatatg ttaaagttag
     1801 ttataagtca aagattttaa gaaggaaaat ttaatgagac acacagttac acgccttttg
     1861 ccagaggcat attccagact ggcagcaatc aaagacaaat acccagccat gagccacaca
     1921 gcacttgttg gtgagatttt gaaggaacac atagccctta caccgtttga cgctatcaag
     1981 ggcgttgcag agggttctaa ggccatcaga atcacaacgg gccaactgga actactcaaa
     2041 gcacagtccg cagtggcagg ttgctcaatg attcaggtgt tgagtgccaa ggttcatcaa
     2101 ctggcggata agctgggggt ttaatgctat gcacatttga ttatcatgcg ttttatcgcc
     2161 atagaggtca ccttgtccgt gagaataacg gaacatggaa agatttaact cgtaatgctg
     2221 aacccttcac acaccagcaa ttaaaatccc gcttctccag atggaagaat gcaccagcag
     2281 atactggtga ttttgagatt atccatacct tcgattatgc ctcagaagat gaccttcacg
     2341 atgcaattaa gctgattgaa gacttcaata cctcagaaga tgtggatggt gacaaatata
     2401 aatcccgtgg taaccgctat aaccgtgatg atatcactca ggccataaat gctggtcaat
     2461 ctcagcgata cattacagaa aaatatggta tttcacggag acagtatttc tacattaagc
     2521 cattgattaa taaagacaat taaaaataac catctaagaa acggtagaat gcactttaca
     2581 ggcagagttg caccttaatt tgataaatta ccttaccagc cagtgcaact ccctctgtaa
     2641 cccgcatggt tactggcctc agaatggcta ttttaagagg tctgaggtgt gtttcatagg
     2701 agatttaaaa atgaataatt acccaatgca aattatggtt gataacgaca ctgctatgat
     2761 ggtgcaaagc ttcattgatg caggtatcgc aattaatttt gataaattac taaagctgat
     2821 ggctgataat gtagaaaaca tctctgactt tattcagtcg gtggagttta acgagcctcg
     2881 aatgatgcta ccaattacag acagcaacat gaaaagattg gttatcgagc aaacgaacat
     2941 atacagtatt tccccagagc aatttttgaa aggtgctgtg acaatcctgt acagtgataa
     3001 tattctggtg acggattctg tgagggttca ctaatgggtt taataattgc ttggacgttg
     3061 cttggcttat ttgttggttg tattattaac aatgcggtaa ataagaattg atgttatcaa
     3121 cctgttaaaa agctgttgac aaataacctt aagcatggca taattttaat tatgggagag
     3181 caattctctc ttatttttct ttcaataatc cgatggatgg atattctgag ttggaaaggt
     3241 gcaatgcttt tcgtcatggc ctttcaggtt agtctgagag gctatctcaa aaagtactct
     3301 tgttcattca ttattgaatc ctttgccctt cacagaaatg tttagggctt tttcagtttt
     3361 taaaagcaca cttggcaggt tcttgttcag tcagactctt cacctctgat tgctgagtgt
     3421 gcttctataa attgaaataa caaataaact aaggggtgaa cgaatgatta gaaatgaaca
     3481 tgaaatgctt atcgaacaca gaccaagtgc acttgatatt cacgaattaa ctttgcgtga
     3541 atcttttgat agagcttggc gggaatcaca cgaagtaaat aaaggtccat atattccacc
     3601 agaaccactc gagattccaa gagttgaaat tgatttttct atgaacgaac ggtgtgaact
     3661 tgatttgaag ttgaagctac aacgccgata tttccaagct attaaagact ctcaagaagc
     3721 aatgagaagt gtttacgaga aaattcagtc caagcttgaa gacgaaattg acgaaatggt
     3781 tatttcgatg gagcttcaaa ccaattctta tgcttatttc tctcgactag accgtgacgg
     3841 ttgggggtat gaccctgtag agattggtaa agtgattgca gatttaccag aaggtcaaag
     3901 agttgttcgt acagaaagag ctgtcagagc accacatcga ctgtctctaa ttgttgctga
     3961 taaatcacaa gctgagcttg aagaaattgc caaagagaaa ctgatgaaag caagagaaaa
     4021 tgagattaat ttgctgaaat caagactggc taagctga
//
LOCUS       DAJVYM010000223         4043 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVYM010000223 DAJVYM010000000
VERSION     DAJVYM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.226"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1599
                     /locus_tag="OQS60_003780"
     CDS             <1..1599
                     /locus_tag="OQS60_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS2389414.1"
                     /translation="MEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLA
                     LHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILR
                     EIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCG
                     NDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIR
                     HTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFD
                     VTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDEL
                     IPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIK
                     QIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSN
                     VINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYME
                     SLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(1833..3116)
                     /locus_tag="OQS60_003781"
     CDS             complement(1833..3116)
                     /locus_tag="OQS60_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS2389415.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3251..3943)
                     /locus_tag="OQS60_003782"
     CDS             complement(3251..3943)
                     /locus_tag="OQS60_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2389416.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT          962 a         1012 c         1041 g         1028 t
ORIGIN      
        1 atggagttcg ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac
       61 gtgatgacca cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat
      121 aactggctgg cgctgcacgg tcgcggccag gattactgcc agcttaaccc tcaaccgatg
      181 gcgtactacg atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg
      241 ccgttccacc cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt
      301 ctgcgtgaga ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg
      361 ctggataaag tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct
      421 ggcggtaact acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc
      481 aatgacacct tctcgctggc agtttacccg tcatcacagc cggtgtttat ggatctcgcc
      541 aaaaaaggtg tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcgttctgc
      601 ggcccatgct ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc
      661 acgcgtaact tcccgaaccg cgaaggctct aagccagcta atgggcagat gtcagcggtg
      721 gcgttgatgg acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct
      781 gccagcgaac ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat
      841 aaaaaccgtg tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacggg
      901 ccgaacatta aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg
      961 tgctcgaaga tcctcgacga agtgaccacc accgacgaac tgattccttc cggtgaaacc
     1021 tcttcttatc gttcaaatcc gattggtctg gcggagttta ccctgtctcg ccgtgatccc
     1081 ggttatgttg gcagaagtaa agcgactgct gagctggaaa atcagcgtct ggcggggaat
     1141 gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga ttgctggtca ggagcatatt
     1201 gatccgctgc aaactgaaat tggcagcatg gtctatgcgg tgaaaccagg cgatggttct
     1261 gcgcgtgaac aggcggcgag ctgccagcgt gtgattggcg gtctggcgaa tattgccgag
     1321 gagtacgcga ctaaacgcta ccgttctaac gtcatcaact gggggatgtt acctctgcag
     1381 atggcggaag tgccaacctt tgaagtgggg gattacattt acatccctgg cattaaagcg
     1441 gcgctggata atccgggtac gacgtttaaa ggttatgtga tccatgaaga tgcgccggta
     1501 acggaaatta cgctctatat ggaaagtctg actgctgaag agcgcgagat tatcaaggcg
     1561 ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa aagcgccatg tgaatgtagg
     1621 tcgcattcgg cacttattgt cggatgcgat gcttgcgcat cttatccgac ctacgaatcg
     1681 catcgaatct gtaggccaga taaggcattt acgcagcatc cggcacttat tgtcggatgc
     1741 gatgcttgcg catcttatcc gacctacaaa tcgcatcgaa ccgtaggccg gataaggcgt
     1801 ttacgccgca tccggcaaat agttaattgc tcttacttct tcgcctctgc aaccacttta
     1861 ctacccacgc cgcggttatt gtattcccac atgcggttgt agttagtgtc attcagattg
     1921 cgctgtattt cgtcgttatc atctacgctg ccggtattac ccgcaaacgg acgattagag
     1981 atcaccgcat cggcccacgg tttagccgtg ttaaaacctt cgttgatggc gctatcacgg
     2041 atcaccacct gaccgttggt attggcatca acatccagcg agcggcccag ttgcgccaca
     2101 ccatcaccga aagcattgaa acggctgttt acggcgagga aaccgtagta catgttggac
     2161 agcgtagccg gtgcaaacac atacgcttct tgctgagtac gtgagttcac cacgcggaat
     2221 tcggtgttat cgaacaccac tgcgccgcga ccagaaacga tatccacatc cccttcaatg
     2281 tagctgttgg tcaccagcgt acgcggctga cgattcgttt ccagacggtt ctgcacaccg
     2341 ctgttggtga caaagaaggt gttctgacga ccgagaatgt taacgttgtt aatctgtacc
     2401 tggtcaccat cagtacgcag tgccaccgcc ggatggttac ctgcatctac gctatcgccc
     2461 agcgtgtttt cgatggtcag attttgcagt tgcaggccat tgttttgtga ccagaagacc
     2521 gcagagcaga gaacaccgat actgtcgctg cgtttgctct ggcagctatc gtacatatac
     2581 cacgctggtt tacctggcat atatttgccg cgcgggttga cgtcgtgacg ccagtcggca
     2641 gggctcatgc caccatcaag ggaaagccca atcttcacat caatcggttt ttcacctgta
     2701 ccgtacagag taattccacc cggagcggca gggacatata ccgttccctg atactcacca
     2761 ggcatcacgg caatatactg gcgcttgttg gtacgcttga taattgccgc atctaccgcc
     2821 gcctgaatcg tggtatgcgt tacaccttga gtgcccgccg ggccgacaac aaagtcaggt
     2881 tgcgcaggca gggtaatcgg ggaaggattc cacgctgcag cacctggtgt cagggatgca
     2941 aaatagtgtt gagcatcgaa attctgcgct tcttttgccg acagaatcgg gcgagaagag
     3001 gtaccaggcg cggtttgatc agaaggacgt tgatcgggcg gggttgagct acaggcggtc
     3061 agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa ctgaaaatgt gttcacaggt
     3121 tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc ctgctttttt atactaagtt
     3181 ggcattataa aaaagcatcg cttatcaatt tgttgcaacg aacaggtcat tatcagtcaa
     3241 aataaaatca ttatttgatt tcaattttgt cccactccct gcctctgtca tcacgatact
     3301 gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg agcaaactta tcgcttatct
     3361 gcttctcata gagtcttgca gacaaactgc gcaactcgtg aaaggtaggt ggatcccctt
     3421 cgaaggaaag acctgatgct tttcgtgcgc gcataaaata ccttgatact gtgccggatg
     3481 aaagcggttc acgacgagta gatgcaatta tggtttctcc gccaagaatc tctttgcatt
     3541 tatcaagtgt ttccttcatt gatatcccga gagcatcaac atgcaatgtt gtagggatgg
     3601 caatttttac gcctgttttg ctttgctcga cataaagata tccatctacg atatcagacc
     3661 acttcatttc gcataaatca ccaactcgtt gcccggtaac aacagccagt tccattgcaa
     3721 gtctgagcca acatggtgat gattctgctg tttgataaat tttcaggtat tcgtcagccg
     3781 taagtcttga tctccttacc tctgattttg ctgcgcgagt ggcagcgacc ggttttgttg
     3841 ttatatggcc ttcagctatt gcctctcgga atgcatcgct cagtgttgat ctgattaact
     3901 tggctgatgc cgccttgccc tcgtctatgt atccattgag cattgccgca atttcttttg
     3961 tggtgatgtc ttcaagtgga gcatcaggca gccccctcct tattgcttta attttgctca
     4021 tgtaatttat gagtgtcttc tgc
//
LOCUS       DAJVYM010000224         3988 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVYM010000224 DAJVYM010000000
VERSION     DAJVYM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.227"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(86..2437)
                     /gene="ygjK"
                     /locus_tag="OQS60_003783"
     CDS             complement(86..2437)
                     /gene="ygjK"
                     /locus_tag="OQS60_003783"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS2389417.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(2454..3531)
                     /gene="ygjJ"
                     /locus_tag="OQS60_003784"
                     /pseudo
     CDS             complement(2454..3531)
                     /gene="ygjJ"
                     /locus_tag="OQS60_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(3665..>3988)
                     /locus_tag="OQS60_003785"
     CDS             complement(3665..>3988)
                     /locus_tag="OQS60_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389418.1"
                     /translation="AASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLI
                     AIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATP
                     ASNVD"
BASE COUNT          892 a         1069 c         1031 g          996 t
ORIGIN      
        1 acgcttgccg cgttttatcc ggcctggggg attgcacatg tagggcggat aaggcgttca
       61 cgccgcatcc gccagccgtt gcaaattact gcttacggaa aaaatcgtta tacaacatat
      121 acaaatgagc ggcactccag gagaaatttg gtgcgccttg ctgtgcgcca gtcagcgggt
      181 tgtaattctc ctgaattggg ccatcggcgg ttaatccttt ggcgtgccgg aagaacgtat
      241 ccgccagctt cagggcatca tcacgataac cgtaacgctc catccctttc agaccaaacc
      301 agaactgatc cacccatacg cgcccacgcc agtagatatc agcgccaaaa gccggattgg
      361 ttaacgccgc cgttcccagc gggacaaagg tgttgaactc tttaggatcg agcatcacct
      421 tcaccaccgc gtcggcgttg gcctgcgttg ccgcaccgtt aaacagcggc aaccagcctt
      481 ccggcccttt accgcgctca acaatcggtt tgcccgcgca gccgttcgcc agtggtttat
      541 cttcaatacg tacgtcatag tagaactggg tagtcgggtc gaacatacag gtgttgatgt
      601 agtccgcgag ctgctgtgcc aactggcgat agcgtttggc ctcttctggc ttaccgagga
      661 tggttgccat ctccgccaga taatggttat cgctgtacat atagctggcc tgatccaccg
      721 actcctgcaa tagcgagtag cccagcaacg ttccgtcctg actgcggttt tcggcgaatt
      781 tcaccgtcca gtcgctacgt ttgccgccat tagcgacata tttatccagc tgttctttgt
      841 cgataaaccc aaagacggcg gcatcatcac gccccgattc ccacgatgca gcaacctgtg
      901 ccggaatttc cagactgtcg tacaggcctt tctccaccac gcgggcgtag ttgttcagcc
      961 cagactgcgt ctcttcttta tcgccttttt tcaccgtaaa cagcatctcg ccgctctcag
     1021 tgttgtgggc tttgtcgcgg gtcgcgccat attccggcac gccgttgccg ttgtgatcgc
     1081 ggttacgtaa ccaccagtcg tgataggcca ccagtttcgg gtacatctct gccaaccagg
     1141 ctttatcctg ggtgacgttg tacacttcca tcaccgacca ggcggcaagg ctgggtttgg
     1201 tattacgttc gttccagttg ccgccatcgc caccacgctc ggggctaagg ttccacgcta
     1261 tcaggtcggg gacaaagccc acatcctgcg gacgcacgct atcgccaggc tggatctgcc
     1321 aggagaagac cgcgcggata ttctctttgg cgatgtccgg attgaaatgc gccatcgcaa
     1381 acgcctgctt ccaggtatcc cacggccagg tctgattgcc ggagaaccag cgcccggtca
     1441 ccgacggtgt aacggtgtta aatttcaccg caccgcccgg tgagagccag ttaccgttga
     1501 gcgtttcgat cgctttcacc gcgacgcgcg tctgttccgg cgtcgcatcc ggattggtta
     1561 accctttctt caggtattct tcccagcgtt gctgcgaggc ggtgagataa aacgccggac
     1621 gcgccagaat atcgcggatc tgcatttgct ctttgctaac ttcctgagcg gtcagcagat
     1681 gggaataggt ggtgtagagc gtggtcgaac cgttgatatg cgccttactg gtaaagcgat
     1741 taccgttgat ttcagtctgc accggcaggg atttatgcac ctgatattcc gattcgccgg
     1801 aggtcagcag atcccaggtg gcgcgcactt tgccaaaggt gactttcagg ccatcacggg
     1861 tggcgctgat tttgcgctga tagtcagggt attcgccagc aatggtttta tcggaaagcg
     1921 gtttcccttc tttcgcttcc agtttttcca gcagttcgcc gtcccacacc agatccagcg
     1981 gtttattgct ggtgattttg gtttccagta gtgacgtgcg cggcgtggcg aagcgcagag
     2041 tcatttcgac ttgcacatct tttgctgtca gtttttgcac cagcgcaccg ggaatactgt
     2101 atgcctccag cgtaaagtcg actttcttgc cgtcctgcca gacggtcagg cggtcgaaat
     2161 tgctggccat aaagttgatg tactcttccg tcagcagcgc aacgcccgga aagccgccca
     2221 tggtgttagg gccgtctggc aacagatgac catgccaggc tccgagatcg aaaaacggat
     2281 taaaacgctg gtgatcgtcg taatcgtaat ccttcatgta ctgcggcgcg ccagtacggt
     2341 taatcacgtt tttataattg tcggcgttag cggcatgggc ggaaaaactt atcagcagag
     2401 cgcaggttac tggcgttaaa atagttttta ttttcatgaa attacagtcc ttattaccag
     2461 tagaaatatt gcatcaggaa gatttggtcg ctgggatcgt gagaggcatt tttcatgtag
     2521 aagcggctat caaaagccgt ggcgaaacgt ccgccgccgt attcgtacca gataccaaac
     2581 tgcatgaaag aggtggtttc atcgtcagcg tggtcagggc gatgtttggc gtaggtgtaa
     2641 gcggtggaga gataaacgcc ctttaacgct tcatacatgg cgctcaccat catcccactt
     2701 tcacttcccg gtacgtccag cccgtcggcg cgtccggtat ggtgccaggc gcgagcggca
     2761 aagttaggag ttagcaggcc cgtgagatag agctgtcctg tgccgtcggt gatttccaga
     2821 ccgttcatcc aggtgatgcc ttcggcaagg tcatactgga tgtaaccgtt catcattgcc
     2881 ggataggtgt atttgtcgtc atagcgatcg tatttgccaa aatgtagcca ggctttgctt
     2941 tcatcatgat gtccggcagg agtcgcggtg acgctataac gaaagttgcc gcttaagttc
     3001 tgtaccttca gcgcagtcat aatgtcgcgg gtattgggaa tgacgtagcc cagatcgggg
     3061 gtgaaatcgc cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg
     3121 atttcggtgc catcattggt acgataaccg ccatagaaac ggttaattcc gccctcgaaa
     3181 ccaccccagc tatggtctgg cacccaggca ttgccgtcgt ggtcggcctg cacggtccag
     3241 ccttcgccgt aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata
     3301 taggtaccgt cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaccc
     3361 cctaatccct aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcggg
     3421 tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta
     3481 gcaaaacggc agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgttaat
     3541 ccatgagcta aagaaaaagt acagcctttt atcgttgtaa ttataaaaat ataaaggcag
     3601 aaactatttt ccctctcctc atccaggaag agggaaaatg cctgctcatt ttttattatt
     3661 ctgcttaatc aacattgctg gctggcgtcg cttcaatatg gcgctcttca gccgttaatc
     3721 cctttatata tttactgtat ttccaccacg caaagccaag gaagataaca ataccgccga
     3781 cgttataaaa aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc
     3841 ctacggcaaa tatcgcaatc agcattgaaa caacgataat accggtgcgg cgggagccca
     3901 tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa gccagcatga
     3961 tgaataacgg cggaagcatg gacgctgc
//
LOCUS       DAJVYM010000225         3975 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVYM010000225 DAJVYM010000000
VERSION     DAJVYM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.228"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            168..503
                     /locus_tag="OQS60_003786"
     CDS             168..503
                     /locus_tag="OQS60_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2389419.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="OQS60_003787"
     CDS             496..978
                     /locus_tag="OQS60_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389420.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="OQS60_003788"
     CDS             complement(1012..1092)
                     /locus_tag="OQS60_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389421.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="OQS60_003789"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="OQS60_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="OQS60_003790"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="OQS60_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="OQS60_003791"
     CDS             2697..3188
                     /locus_tag="OQS60_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS2389422.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..>3975
                     /gene="ygeH"
                     /locus_tag="OQS60_003792"
     CDS             3523..>3975
                     /gene="ygeH"
                     /locus_tag="OQS60_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCS2389423.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT         1489 a          635 c          639 g         1212 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tcgat
//
LOCUS       DAJVYM010000226         3909 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVYM010000226 DAJVYM010000000
VERSION     DAJVYM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.229"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            24..590
                     /locus_tag="OQS60_003793"
     CDS             24..590
                     /locus_tag="OQS60_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS2389424.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="OQS60_003794"
     CDS             594..1169
                     /locus_tag="OQS60_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS2389425.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="OQS60_003795"
     CDS             complement(1211..2263)
                     /locus_tag="OQS60_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS2389426.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="OQS60_003796"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="OQS60_003796"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS2389427.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="OQS60_003797"
     CDS             3580..3777
                     /locus_tag="OQS60_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS2389428.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          987 c          951 g         1000 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctgat
//
LOCUS       DAJVYM010000227         3885 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVYM010000227 DAJVYM010000000
VERSION     DAJVYM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.230"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OQS60_003798"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OQS60_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OQS60_003799"
     CDS             1909..3261
                     /locus_tag="OQS60_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCS2389429.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OQS60_003800"
     CDS             3285..3767
                     /locus_tag="OQS60_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS2389430.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         1042 a          997 c         1051 g          795 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggg
//
LOCUS       DAJVYM010000228         3865 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVYM010000228 DAJVYM010000000
VERSION     DAJVYM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.231"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            63..1226
                     /locus_tag="OQS60_003801"
     CDS             63..1226
                     /locus_tag="OQS60_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS2389431.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OQS60_003802"
     CDS             complement(1686..2123)
                     /locus_tag="OQS60_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389432.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3865)
                     /locus_tag="OQS60_003803"
     CDS             complement(2212..>3865)
                     /locus_tag="OQS60_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCS2389433.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     LASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNL
                     LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKN
                     NPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLN
                     AFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          920 a          912 c          948 g         1085 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaattg ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc tccaccgtca ggccgcacga tgccagccag cggcggcttt cctcactcac
     3061 ccggtcagcc aggatttcac tttccagccg gtcgagcatc tgcaccagca ccggcgggaa
     3121 caccacactg ccaccgtctt cgccgccgga ctgctgaagg gcatccgcca ggctgcggcg
     3181 ctgcgttttc gccagctcac cggtggcagt ttcgaccctg atgagtggcg tgaagctccc
     3241 cggctgataa atcgtctgga tgcgggttct gtcgttctgt attgtggtca gccggtcgcc
     3301 gtcccagccg taccaggtca cttgcggttt ccgtgacagc gacatccagc ccgtcaggtc
     3361 ccgttcacgc cgccataccc gttttgccac cctgcggccc agcgggtcgt aaagatagcg
     3421 actttcgacc agcggctctg catattgtgt ccgcgtgtag tgcaccagcc ggtgctgact
     3481 gtcgtaatgg taccggtggg tgcgctcatc atccgtgcgg ataacccctt ccgggatgag
     3541 gtcggttttc tctgtcagtc tgccgtgatg gtcataccgg taaagatagt gcgcgtcacg
     3601 ggcgatacgg ttatccggcc acatgctgag ggtgctgtcc gggtgcagct ccgggtcggg
     3661 caggcggttc cccgccgggt ctgtggcata cgggatgcgg atatccagat tcgctgcggt
     3721 ggtgtgaacg ccggtcagcc tgccggtggt gctgtagctg taactccggg tctggcgcgg
     3781 gctgctgatg cggatgagtt cgccgttgtc gttccaggtg taatcgcggt cagacagcag
     3841 gctgttcagg tgctggctct gtaac
//
LOCUS       DAJVYM010000229         3862 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVYM010000229 DAJVYM010000000
VERSION     DAJVYM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.232"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..143)
                     /locus_tag="OQS60_003804"
     CDS             complement(<1..143)
                     /locus_tag="OQS60_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2389434.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IIN"
     gene            complement(354..1751)
                     /gene="ccp"
                     /locus_tag="OQS60_003805"
     CDS             complement(354..1751)
                     /gene="ccp"
                     /locus_tag="OQS60_003805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS2389435.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2156..3805
                     /locus_tag="OQS60_003806"
     CDS             2156..3805
                     /locus_tag="OQS60_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS2389436.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1006 a          979 c          905 g          972 t
ORIGIN      
        1 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
       61 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
      121 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
      181 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
      241 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
      301 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
      361 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
      421 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
      481 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
      541 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtaacatt catacgacca
      601 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagccccaa cggttcaaag
      661 gaacgtccac cgagaataat accaccatga caagttgcac atttattatc tttaaataat
      721 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
      781 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
      841 tcgccactga aaccttgcgg atagacgccg aggaactgcg ctttaagctg cggatctttt
      901 tccagcttag caataatttc gtcccaagat ttcgacgcca tttcaatcgg gttcaacggc
      961 ggtccaccag cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1021 aatactgagt taaataccgt cggcgcgtta atcggcccaa ctgcgccacc aacaccaatc
     1081 gatgtttttc tgccatcgac gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg
     1141 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     1201 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     1261 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     1321 cgctcttcat cactcacctt acccgcccag tgaagcgcgg tataacgcgt tggcggcata
     1381 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     1441 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     1501 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     1561 gtgtggcaat agtcgcatcc tttttcgcgg agaaaaccta aaaccttatt attttcactg
     1621 acagcggatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     1681 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     1741 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     1801 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagtgaga tacgcttttt
     1861 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     1921 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     1981 aaaaacaaca cgaaatccgt atttttataa attccatgga tattagtgcg ttatattttt
     2041 tgacaggtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     2101 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     2161 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaagtcg atctctgcta
     2221 tgagctggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     2281 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     2341 gttcgagcat gttcagtcgg cgaaaatttt cccggacagt aaaacctttc ccgactgcgc
     2401 acctaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     2461 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     2521 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     2581 aacccgcgaa ccacaggatc acattccgtg gtcttctcta ctggcgctgc cgcagtcata
     2641 tattgtccca ggcggccgtt ttagcgaaac ctactattgg gactcctatt tcaccatgct
     2701 ggggctggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     2761 gatgatcgaa aactatggtc acatccccaa cggcaaccgc acctattatt tgagccgatc
     2821 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     2881 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     2941 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3001 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     3061 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggggcggc
     3121 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     3181 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     3241 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     3301 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     3361 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     3421 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     3481 gttactgaca cctggcggga ttctggcaag cgagtacgaa accggtgaac agtgggataa
     3541 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     3601 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     3661 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     3721 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     3781 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     3841 gtaaaattat cctcttcagg ag
//
LOCUS       DAJVYM010000230         3845 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVYM010000230 DAJVYM010000000
VERSION     DAJVYM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.233"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            242..583
                     /gene="raiA"
                     /locus_tag="OQS60_003807"
     CDS             242..583
                     /gene="raiA"
                     /locus_tag="OQS60_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS2389437.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELVNKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    686..811
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            687..734
                     /gene="pheL"
                     /locus_tag="OQS60_003808"
     CDS             687..734
                     /gene="pheL"
                     /locus_tag="OQS60_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCS2389438.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OQS60_003809"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OQS60_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS2389439.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQS60_003810"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQS60_003810"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS2389440.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..>3845)
                     /gene="aroF"
                     /locus_tag="OQS60_003811"
     CDS             complement(3169..>3845)
                     /gene="aroF"
                     /locus_tag="OQS60_003811"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS2389441.1"
                     /translation="ELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREM
                     ASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVIL
                     RGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKD
                     GNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQ
                     LTARVA"
BASE COUNT         1038 a         1016 c          878 g          913 t
ORIGIN      
        1 ttgagctgcc gtttttttat tctgttagtt gtgaaactga agcgatttag tcactatcga
       61 tctcatcaaa tatggctcgc tttgagatat tcctcaagta aaaaaacacc tcttcctgcg
      121 atttctcaca aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt cccatcacac
      181 attttgacat caggaacggt atgctgaatt caccaagacg ggaagacaag aggtaaaatt
      241 tatgacaatg aacattacca gcaaacaaat ggaaattact ccggccatcc gccaacatgt
      301 cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc cacatatcat
      361 tctgtccaaa gagccacaag ggtttgttgc tgacgccaca atcaatacac ctaacggcgt
      421 tctggttgcc agtggtaaac atgaagatat gtacactgca attaacgaat tggtcaacaa
      481 gctggaacgg cagctcaata aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc
      541 ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgg
      601 atcgccaacg cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg
      661 tactaaagtc acttaaggaa acaaacatga aacacacacc gtttttcttc gcattctttt
      721 ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat
      781 aaggcctccc aaaccggggg gcctttttta ttgataacaa aaaaggcaac actatgacat
      841 cggaaaatcc attactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cattactggc agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgagatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc agcaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cacgactgat ttatccacca
     1441 ttaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atcctcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcgaatc agcaacaaaa cttcacccga tttgtggtgt
     1681 tggcacgtaa agccattaac atgtctgacc aggttccggc gaaaacgacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgctggttg aagcgttgct ggtgctgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatatttt
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaattac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcggttgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat aatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag atgcagccca taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcag ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatgacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaa
//
LOCUS       DAJVYM010000231         3756 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVYM010000231 DAJVYM010000000
VERSION     DAJVYM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.235"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            115..528
                     /locus_tag="OQS60_003812"
     CDS             115..528
                     /locus_tag="OQS60_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS2389442.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OQS60_003813"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OQS60_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS2389443.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OQS60_003814"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OQS60_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="OQS60_003815"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="OQS60_003815"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS2389444.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OQS60_003816"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OQS60_003816"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS2389445.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OQS60_003817"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OQS60_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS2389446.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
BASE COUNT          994 a          953 c          944 g          865 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaaga
//
LOCUS       DAJVYM010000232         3734 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVYM010000232 DAJVYM010000000
VERSION     DAJVYM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.236"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..181)
                     /locus_tag="OQS60_003818"
     CDS             complement(<1..181)
                     /locus_tag="OQS60_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCS2389447.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="OQS60_003819"
     CDS             425..682
                     /locus_tag="OQS60_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCS2389448.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OQS60_003820"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OQS60_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS2389449.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OQS60_003821"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OQS60_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS2389450.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OQS60_003822"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OQS60_003822"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS2389451.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="OQS60_003823"
     CDS             complement(3307..>3734)
                     /locus_tag="OQS60_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS2389452.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAJVYM010000233         3572 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVYM010000233 DAJVYM010000000
VERSION     DAJVYM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.238"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..302
                     /locus_tag="OQS60_003824"
     CDS             <1..302
                     /locus_tag="OQS60_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389453.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            391..2259
                     /locus_tag="OQS60_003825"
     CDS             391..2259
                     /locus_tag="OQS60_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCS2389454.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2296..>3572
                     /locus_tag="OQS60_003826"
     CDS             2296..>3572
                     /locus_tag="OQS60_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS2389455.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDT"
BASE COUNT         1132 a          653 c          689 g         1098 t
ORIGIN      
        1 cacgtgtaga actgtattac cgctttaacc atgaagaaga atattaccct tctcaccgtt
       61 ttatgcttgt tgttgatatg aagacagtaa tggttttttc acaacgagct gaatcactgg
      121 ggttatactc gaataatcgc ttgcttatca atataattgc cgaacatatc catagtgata
      181 tatatctaac caagtatcag caacaatatc cagatgccaa tgcattaata aatactttgc
      241 atgaatctgg caatgatatg gtgcaggatg aaaaacattt gagtgatagc aaaaaccgtt
      301 aatattttcc tttcattgtg atcgtctgta ggcgatcaca atctatttgt cttttataac
      361 gtctaaactt ttttatgcaa aggtgtattt atgatttttt ttaacgatat aaagcccact
      421 ggttggttaa agaataagat ggaatggtat atgaacggtc atataggaga actggataaa
      481 ttagttcccc atcttattac ggagcataaa atatatggaa gagatcgtat aactccacgc
      541 actgtatctg ctgaagatgg cgttattaaa aaacagaata atcccgatga taacatgatg
      601 caatattatt ggtgggatag tgaaactcaa tcgaattgga aggatggata ttgccgcagt
      661 gcctacttgt tagataataa agaatggcag gaaaaagcga gcgatttatg tttggaactt
      721 atcgatacgc aagatgatga tggctacatt ggaatttatg attcatcatt aaaatttaac
      781 tgtaaagcag aaaacggaga gttctgggca caagctactg ccttgcgttt tgtgcttgga
      841 tgttatgagt ccacccgtaa tgagagttta ttaacttctc ttacccaagc cgcacaatgc
      901 attatggctg gctaccctaa agaaacgtca catcctttcg ttgtggaaca gggttttgct
      961 ggacatagcc acggattaac aataacagat gttttctacc gcctttatga aataaccaat
     1021 aacattgaat atctctcata ttgcttgtgg ctgtatgaag attattccgc aggatgcgtg
     1081 tcggagcaag atctgcaata taaaaatgtc atagaccctg gttaccttct taaaggtcat
     1141 ggcgtacata cgtatgagca tattagggca ttagccatcg cagcgagcca acggccgcag
     1201 tatcaaactg cattagatat ttttttatcc cagttgaaat attatcttac accatctggc
     1261 gctcccattg gtgatgagtg gattacagga cgcacagcta atgccactta tacaggctat
     1321 gaatattgtt ctgttcatga actatttagc acttatgtta tgttaataaa actgaccact
     1381 aatattggtc tggccgatga tgctgagtgg ctattctata atgctagttt tggcatgcag
     1441 catcccatac ttcacgcgat aatgtactgt aagactgata attgttatga agcaaatgaa
     1501 aggaaacatc ctgacagtaa tcagtttaat accagatata aatactcccc tactcatgag
     1561 gaggctgctg tctgctgcgt accgaataca gcaagaacca taccggcttt tgtcgaaaat
     1621 atgtttcaag aaaataacaa tggttttact gcattatttt atggcccgtg tgagttttat
     1681 ggcacattta ataatgttgc aattaaaatt acacaattaa ctgaataccc tcatgattta
     1741 agcgtaaaat gtctcattga gcctgagtca tctgttcgtt ttgctctcag tttccgttac
     1801 cccggctggg caaaaatgat gattattaat ggtgttactt ttactaaaaa tgatactcaa
     1861 aactcactta ttattttaga tagaacatgg caacatcatg atgtgattga cattaaattt
     1921 atagctgata tacagtttaa tactgactta tgtggtgaca cttatatttc cagaggtccc
     1981 gttttatatg caattgagct tgagagcgat attttaatca aaaaaaacct gctgaacggg
     2041 aaatattttg actcaggata cataccctgt agtagagctg atgaatctat ccagttctct
     2101 tatgctgata tggaatcttt ttcatattca acctcaccag agggcaaaca atacattgaa
     2161 ggctgttttt atttaaacaa gactaaaata gtcagaaaac ttataccatt tggtcaaacc
     2221 attcttcgca aagtaacttt ctccaatata gagagttaat attttcgagt gttataatta
     2281 attaaaggga tttctatgta ccttactgat aatattatca aactgtcgaa taaaataggt
     2341 cagcgtgttt atactgttgt ctctgaaatg tcgattgaag catggataac aaaagaaccg
     2401 cagccttttg tttatcgccg tagcggcgct taccagaaac tcaccattgg ctcagactgg
     2461 ggtcaactgt ttgactgtgc ctggatgcga gtttatggta aacgtccagc agataaattt
     2521 agacacaaac tcgtcgctct cgttgatatc ggtggcgaag gtcttattgt ggataagaat
     2581 ggttcaccca tctgcggcat cacgaataaa gcatcatctt atggcgtacc gccagataaa
     2641 ccaggtaaat gggtggttga cctttctctc gttagtgaaa atgacgaggt tgagttttgg
     2701 attgatgctg cctgcaacga tttatttggc tatgttacta atggagggat tattactgat
     2761 gttcatatcg cgacacgtaa tcaactactg aaatcccttt attatgatat tgaggtgctt
     2821 ttcgactgga ttaatgatgg tcagaagttt gaatccatac atcctaaagg tatcacccca
     2881 gaacaattta ttgccaaaag aagtgagcgt actgacgaaa tcataagtac tctcgaatat
     2941 attgataata cgttaattac ctttagcaat gaagaaataa ttaaatgtca aattgcaatc
     3001 caatccataa tcaataaaaa caataatcct tcagagttca ggattatggc tactggtcat
     3061 gcccatttag atatagcgtg gatgtggcct ttaagagaag gacgcagaaa agcaataaga
     3121 acatttgcca ccgcccttgc aaacattgaa aaatatcctg attacatttt tggcgctagt
     3181 cagtaccaac tttttcactg gataaaaaaa gattatccct atttcttcga aaagttaaaa
     3241 aaacaaatcg ccgcaggccg ctttgagtta caaggttgct tctgggtcga atgcgattta
     3301 aatctggtaa gtggtgaatc gctgataagg caaatcatgc atgggacacg attcacactg
     3361 cagaattttt caaaaaagat aaattatgtc tggcaaccgg atgtctttgg attccccgca
     3421 acgcttccac aaattctcaa aaaatcaggc attaactata ttgcatcgca aaagctctca
     3481 caaaacaaga ttaataaatt taataattac cttttccgct ggcagggcct ggatggctca
     3541 gaaattctga tgcataactt tcctgaagac ac
//
LOCUS       DAJVYM010000234         3570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVYM010000234 DAJVYM010000000
VERSION     DAJVYM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.239"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQS60_003827"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQS60_003827"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS2389456.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQS60_003828"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQS60_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS2389457.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQS60_003829"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQS60_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS2389458.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          914 a          897 c          910 g          849 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agctaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccgatgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgtggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aataaacgta
     1861 aggtgtcgcg aatgcgtcgc aaaaagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa
//
LOCUS       DAJVYM010000235         3551 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVYM010000235 DAJVYM010000000
VERSION     DAJVYM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.240"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..324
                     /locus_tag="OQS60_003830"
     CDS             <1..324
                     /locus_tag="OQS60_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389459.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="OQS60_003831"
     CDS             551..1816
                     /locus_tag="OQS60_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389460.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="OQS60_003832"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="OQS60_003832"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS2389461.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="OQS60_003833"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="OQS60_003833"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS2389462.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT          909 a          792 c          858 g          992 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata a
//
LOCUS       DAJVYM010000236         3544 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVYM010000236 DAJVYM010000000
VERSION     DAJVYM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.241"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..141)
                     /locus_tag="OQS60_003834"
     CDS             complement(<1..141)
                     /locus_tag="OQS60_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389463.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(187..528)
                     /locus_tag="OQS60_003835"
     CDS             complement(187..528)
                     /locus_tag="OQS60_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389464.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(697..888)
                     /locus_tag="OQS60_003836"
     CDS             complement(697..888)
                     /locus_tag="OQS60_003836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389465.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(821..2152)
                     /gene="xylB"
                     /locus_tag="OQS60_003837"
     CDS             complement(821..2152)
                     /gene="xylB"
                     /locus_tag="OQS60_003837"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS2389466.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2224..>3544)
                     /gene="xylA"
                     /locus_tag="OQS60_003838"
     CDS             complement(2224..>3544)
                     /gene="xylA"
                     /locus_tag="OQS60_003838"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS2389467.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          923 a          983 c          783 g          855 t
ORIGIN      
        1 tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata gccctaacag
       61 ataggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg ccggtgaata
      121 ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt aagagcttta
      181 tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa aaaaggcgat
      241 ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga taaacaagag
      301 accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat ccagttgccc
      361 gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta acacgccaag
      421 aaaatagcct tttccactta gcaatgggca tgagatccat agcccaacca gataaaccag
      481 cgccccgaca acaaataata gttttgccac agtttttgag gttttcataa cgatctccat
      541 atctaccagc gatacattac gagtaaccaa cgaaagacaa aactgaagaa tgccattaac
      601 aaatgatttt cagaataaat tcatactaaa tattaattaa ttagtgagat atatagatgt
      661 gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg gcagaagttg
      721 ctgatagagg cgacggaacg tttcacgtcg tggctgataa gcggcataac gctgcgcatc
      781 tggtagatgc gactgttcta acggtagctg cggcaacaac tcaatgagcg atttctctgg
      841 attcgccgca atctgcgcca gccttgctgc gcccagtgct ggccccacat cccccccccg
      901 tacggtaatc gagcttctga ccgctgatat ccgccagcat ctgacgccag tactcactac
      961 gcgccccgcc accaatcaac gtaacacttt gcggtttaat gccgcaggca tgcacgacat
     1021 ccatgccatc tgccagcgca taacccacgc cttccagcac tgctcgcgcc agttcattgg
     1081 ggccatgttg atgagtcaaa ccaaagaaaa cccccttcgc ctggggatta ttgtgtggtg
     1141 tacgctcgcc ggaaagataa ggcagaaacc aaactggctc gacactttca tcagcctgtt
     1201 gagctgcagc gattaaagct gggacattgc acaggccggt taatttcgcg gcccaatcca
     1261 gacacgatgc tgcactcagc attacagaca ttaaatgcca acgctgcggt agcgcatggc
     1321 aaaagctgtg tacagcgctt tctggcttgc ttaagaaccc ttcgctgaca gcaaaataga
     1381 cccccgacgt ccccagcgat aacattgcct gattagcatc aaccattccc acaccaactg
     1441 caccagctgc attgtcgcca ccgcctgcga caactggcac cgtcgccata ccccacgctt
     1501 tcgcaacttc aggtaacaaa gcaccagtaa tttcgctgcc ttcgtataat gcgggcatct
     1561 ggtcacgaga taagtcgcaa gcctgcagca tgacgtcact ccagtcacgc tttgcgacat
     1621 ccagccacat ggtgccagct gcgtcagaca tatcgctggc aaactccccc gtcatacgca
     1681 gacgcaagta atctttcggt aataatactt tgtcgatttg acggaatatc tccggctcat
     1741 gccgctgaac ccatagcaat ttaggcgcag taaatccggg catcatcagg ttgccggtaa
     1801 tcactcgtga ttgcggaact ctcgcttcca gcaaagtgca ctcttgcgca cagcgcccgt
     1861 cgttccacaa aatggcaggg cgcaataccc gttgttgagc atccagtaag gttgctccat
     1921 gcatctggcc ggcaataccc aatgctttaa cgtcctgcag agaatgctga tcgcccagag
     1981 ctttcattgc gcgatcagtt gcctgccacc actgttccgg gtcttgttcc gaccagagtg
     2041 gatgcgggcg cgaaacggtc agcttttccg tttgcgaatc aaccacctca ccctgctcgt
     2101 tgagcaaaat aacttttacg cccgaggtgc caagatctat cccgatatac atatcgatcg
     2161 ttccttaaaa aaatgcccgg tatcgctacc gataaccggg ccaacggact gcacagttag
     2221 ccgttatttg tcgaacagat aatgatttac cagattttcc agttgctcct ggcgaccact
     2281 ctgatgcacc ggagacaaat tatgttcctg agcatatttg gctaaatctg ccagtgacat
     2341 ttggcctttc aggatttgct ggcccaattc gctattccag ccggaataac gctgcgcgat
     2401 gcgtttatcc agctcgccat cttcaatcat gcgcgctgca attttcagcg ccagtgccat
     2461 cgtatccatc gcgccgatat gaccgtaaaa cagatcatat ttatcagtac tttgacgacg
     2521 tactttggca tcgaagttca gaccaccggt ggtgaaaccg cctgctttga gaatttcata
     2581 catcaccagc gcattctctt ccacactgtt cgggaactgg tcggtgtccc agcccagttg
     2641 cgcatcgcca cggttggcgt cgacagaacc gaacaggcca agcgcaatgg cggtggctat
     2701 ttcatgatgg aaagagtgac ctgccagcgt cgcgtggtta gcttcaatgt tcagtttaat
     2761 ctctttttcc agaccaaact gtttcaggaa gccatagacc gtcgcggcat cgtaatcata
     2821 ttgatgttta gtcggttctt gcggtttcgg ttcgataagc aacgtgccct ggaagccgat
     2881 tttatgttta tgctcaacca ccatctgcat aaagcggccc agttgttcac gctcctgacg
     2941 caagtcggta tttaacagcg tttcgtaacc ttcacgacca ccccacagga catagttttc
     3001 accgcccaat ttatgggttg cttccatcgc tgtaacaact tgcgttgccg cccagctgaa
     3061 gacttcagga tctgggttcg tcgccgcacc cgcgccatag cgagggtttg taaagcagtt
     3121 agcggttccc cacagcagct tcacgccgct ctcttcttgc ttggctgcca ggacatcaac
     3181 catttgcgca aaattattga tgtactcttt taacgacgcg ccctcagggg aaacatccac
     3241 atcgtggaag caataaaatg gcacatgtaa cttgtggaaa aactcaaatg cgacatctgc
     3301 tttacgcttc gccaacgcca gtgcctcacc aggctgctgc cacggacgat taaacgcccc
     3361 cacaccaaac atgtccgccc cgttccagca gaaggtgtgc cagtagcagg cggcaaaacg
     3421 caagtgctct tccatacgct tacccaacac cagttcgtcg ggattgtagt gacggaatgc
     3481 taacgggttt gaggattttg agccttcata acgaacgcga tcgagctggt caaaataggc
     3541 ttgc
//
LOCUS       DAJVYM010000237         3534 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVYM010000237 DAJVYM010000000
VERSION     DAJVYM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.242"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            65..472
                     /gene="nudG"
                     /locus_tag="OQS60_003839"
     CDS             65..472
                     /gene="nudG"
                     /locus_tag="OQS60_003839"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS2389468.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(438..710)
                     /locus_tag="OQS60_003840"
     CDS             complement(438..710)
                     /locus_tag="OQS60_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS2389469.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            946..2289
                     /gene="gdhA"
                     /locus_tag="OQS60_003841"
     CDS             946..2289
                     /gene="gdhA"
                     /locus_tag="OQS60_003841"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS2389470.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2406..3445)
                     /locus_tag="OQS60_003842"
                     /pseudo
     CDS             complement(2406..3445)
                     /locus_tag="OQS60_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          907 a          842 c          847 g          938 t
ORIGIN      
        1 ctttctgatt gagcaacagt gcggatatta tggcattttt cgcttatctg cccgtgtgta
       61 atttatgaaa atgattgaag ttgttgccgc catcattgaa cgtgatggca aaattttact
      121 cgcgcaacgc cccgcccaga gcgatcaggc gggattatgg gagtttgccg atggtaaagt
      181 cgagctggat gaaagccagc agcaggcact ggtgcgtgag ttaaacgaag aattgggcat
      241 cgaagcaact gtgggtgagt atgttgccag ccatcagcga gaagtttcgg gccggattat
      301 ccatcttcat gcctggcacg tacccgactt ccacgggacg ttacaggcac atgaacatca
      361 ggcgctggtc tggtgctcac ctgaagaggc gctgcaatat ccgctggccc ctgctgacat
      421 tccactatta gaggcgttta tggctttacg cgccgccaga gcagcggatt agtgctaagg
      481 gttttgtcat cccgctggca ttgcagcagt attccttcgg ctttaattac cgccccttca
      541 gaataatttt gatcctgata aacgcagcac tgagtacagg gctgcgctga ctgcccgcct
      601 gaactgaaca cttctggcgg tacgtttacc tccacgtccg gacgataatg cgggttagcc
      661 agtgcaatta atggaaatgc taatactacg gcgaacaatg ctcgactcac agggaactcc
      721 ttaacgttat tgtctctgca actgataacg gtagccgggt ggcaaaactt tagcgtctga
      781 gttatcgcat ttggttatga gattactctc gttattaatt tgctttcccg ggtcattttt
      841 ttcttgctta ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc acatgacata
      901 aacaacataa gcacaatcgt attaatatat aagggtttta tatctatgga tcagacatat
      961 tctctggagt cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg
     1021 caagccgttc gtgaagtaat gaccacactc tggccttttc ttgaacaaaa tccaaaatat
     1081 cgccagatgt cattactgga gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg
     1141 gtatgggttg atgatcgcaa ccagatacag gtcaaccgtg catggcgtgt gcagttcagc
     1201 tctgccatcg gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa cctttccatt
     1261 ctcaaattcc tcggctttga acaaaccttc aaaaatgccc tgactaccct gccgatgggc
     1321 ggtggtaaag gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga agtgatgcgt
     1381 ttttgccagg cgctgatgac cgagctttat cgtcatctgg gcgcggatac cgacgttccg
     1441 gcaggtgata tcggcgttgg tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag
     1501 ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg cggtagtctt
     1561 attcgcccgg aagctaccgg ctacggtctg gtttatttca cagaagcaat gttaaaacgc
     1621 cacggtatgg gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag
     1681 tacgctatcg aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc agactccagc
     1741 ggcactgtag ttgatgaaag cggattcacg aaagagaaac tggcacgtct tatcgaaatc
     1801 aaagccagcc gcgatggtcg agtggcagat tacgccaaag aatttggtct ggtctatctc
     1861 gaaggccaac agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc cacccagaac
     1921 gaactggatg ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc cgtcgctgaa
     1981 ggggcaaata tgccgaccac catcgaagcg actgaactgt tccagcaggc aggcgtactg
     2041 tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa cgtcgggcct ggaaatggca
     2101 caaaacgctg cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt gcatcacatc
     2161 atgctggata tccaccatgc ctgtgttgag catggtggtg aaggtgagca aaccaactac
     2221 gtgcagggcg cgaacattgc cggttttgtg aaggttgccg atgcgatgct ggcgcagggt
     2281 gtgatttaag ttgtaaatgc ctgatggcgc tacgcttatc aggcctacaa atgggcacaa
     2341 ttcattgcag ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg caaaatttca
     2401 ggcgtttatg agtatttaac ggatgatgct ccccagggaa catttcttat gggccaacgg
     2461 catttcttac tgtagtgctc ccaaaattgc ttgtcgtaac gataacacgc ttcaagttca
     2521 gcatccgtta actttctgcg atagcagcag atatgccagt aaagaaaccc cattagacta
     2581 tttttttgat aatcttcttc gctttcgaac aactcgtgcg cctttcgaga agcaagcatt
     2641 atataatgcc aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt gcttgatatc
     2701 gaggtcatcc atgataattc cgccgcccat attagcttcg ccgaggattt accggagcga
     2761 ttattagcgc aatcagagat atagtctgag ggaaaaacag caaatttatt caacaaggcg
     2821 ataacctgct ctggggcttc ctccatgttt gctttaaagg tattggctcc atggtcgcca
     2881 gaaagaaaat gctccattag gcacaataac tttcgctatc ttcgataccc cattgattct
     2941 ctaaagactc gcgtctttta ctgatgatat cgatcgagtc aaaaggaagc acatgatatt
     3001 ggaaggtatc tttaccaggt tcaggctttc gcggccagaa ctctagcgtt tcagaccatt
     3061 gcttatgata gaatcgataa ggtgcgatca attgtagcgc ctgtaacttc tcgatactga
     3121 gcggctcaat acctttagcc tgataataat gcagttgttc tttttttgct ttaaaaccgg
     3181 cccgaacaat aagccccatc ataattaata gataaagaaa agagcatccc gcggtaatca
     3241 ggcctctttc attcaaaccg ttggatgtta tcgctgcgaa cacaaacatt acagcgacaa
     3301 cacatgttaa ataaaacccc cacttacaaa gcagcatggc cttattttct ttaatcatcc
     3361 gttcaaaatt actattaaat atttcccagc cattaaaaga atacttctcg ctcccaggat
     3421 ggttttgtaa taaaactttt ttcatcacat tccctgttat tacatacttt agaggcgata
     3481 attttatcca tgcaaaaaaa atatccaacg aattttcttg atccgctgga tatt
//
LOCUS       DAJVYM010000238         3534 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVYM010000238 DAJVYM010000000
VERSION     DAJVYM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.243"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..191
                     /locus_tag="OQS60_003843"
     CDS             <1..191
                     /locus_tag="OQS60_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="regulator of carnitine metabolism; induces the
                     caiTABCDE and fixABCX operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HCS2389471.1"
                     /translation="GCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVRKTPRIPAVPLT
                     ELNREQKWQMMLSKSMRR"
     gene            complement(400..990)
                     /gene="caiE"
                     /locus_tag="OQS60_003844"
     CDS             complement(400..990)
                     /gene="caiE"
                     /locus_tag="OQS60_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS2389472.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(996..1781)
                     /gene="caiD"
                     /locus_tag="OQS60_003845"
     CDS             complement(996..1781)
                     /gene="caiD"
                     /locus_tag="OQS60_003845"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS2389473.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(1890..3443)
                     /gene="caiC"
                     /locus_tag="OQS60_003846"
     CDS             complement(1890..3443)
                     /gene="caiC"
                     /locus_tag="OQS60_003846"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS2389474.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          811 a          993 c          869 g          861 t
ORIGIN      
        1 ggggatgcca gtgccagcga ctggtaaaag tggtcgatat cgatgagcaa atttacgcgc
       61 gcctgcgcaa taacagccgg gataaattag tcggcgtaag aaagacgccg cgtattcctg
      121 ccgttccgct cacggaactt aaccgcgagc agaagtggca gatgatgttg tcaaagagta
      181 tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg ttgctggatg caatgctggc
      241 gcatcttatc cagcctacgg ttgctctcca gccgggttgg ataaggcgtt tatgccgcat
      301 ccgacagcta tcgccggatg cgatgctggc gcatcttatc cggcctacgg ttgctctcca
      361 gcagagttgg ataaggcgtt tacgccgcat ccggcaagat caccgtttag gcgtcacatc
      421 cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc ctcagtggct gtgtttcatg
      481 taacgatgca tgacagcgcc cgacaagatc ctgatactct ttggtattca accgtttcca
      541 gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct ggcgtaccca tcaacaactg
      601 gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg gcggcaacaa tgctctcttc
      661 gccaatgacc gcgccatcca taatcacgct gttcatcccg accaatgcat cgcgaccaat
      721 cacacaacca tgcaggatcg ctccgtgccc gatatggccg ttttccccaa cgatggtgtc
      781 agtgtcgcag tagccatgca taatgcagcc atcctgaata ttggctcccg cttgcacgat
      841 caaccgcccg tagtcaccac gcagtgaggc gagtgggccg atgtagacac cggctcccac
      901 aatcacatcg ccaatcaaga cggcactggg atggacaaac gccgtcgggt gaaccaccgg
      961 aattaacccc tcaaaggcgt aatagctcac ggttgttaac gtcctttcca caccggatcg
     1021 cgcttctcgg caaacgccag cggcccttca atggcatcct ccgaatgcag aaccgacgga
     1081 tagtgtttca acacgccgct gcgaatatag cgatacgctt cttctaccgg catttcgctg
     1141 gtggtgcgga agatctcttt cagcgccgca atcgccagcg gggcgctgtt aaccagctgc
     1201 tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc taaccacgcg gttgactatc
     1261 ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg tcatcaccat ttcattgacg
     1321 atggcaggcg gcaggatctt cggtagacgc agcacgccgc cgctgtcagg aacgatgccc
     1381 agcttggctt ccggcagggc gaagctggcg ttatcggcac aaacaataaa atctgccgcc
     1441 agcgccagtt cgaaaccgcc gtcaaaggca tagccgttca cagctgcgat aaccggtttg
     1501 tcgagattga aaatttcggt taatcccgca aatccacccg ggccaaagtc agcatccggt
     1561 gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg aaaagaactt ctctccggca
     1621 ccggtaataa tggcgacacg taattgcgga tcgtcacgga aatttagaaa tacttcgccc
     1681 atttcaaagc tggtttttgc atcaatagca ttcgcttttg gacgatcaag ggtaatttcc
     1741 agaattgatc cattgcgggt cagatgtaat gattcactca ttccttttct ccatttttgc
     1801 tttttcaggg acgacaacat ccctgcaaaa aatgcatatt gttttatagc gtgattatta
     1861 gttggcaggg tagttccctg ctgtttcatt tatttcagat tctttctaat tattttcccc
     1921 gagcaattac gtggcagatc ttttctgatc tccagataag agggcacttt aaatttcgcc
     1981 atattttgtt cgcagaagcg gaaaaattcc tcttcgctca atgtttcacc ttcattcagc
     2041 accacaaatg ctttgatggc ttcatcgcga atcgaatctt taatacccac aaccacgata
     2101 tcctgaattt ttggatgggt ggcgatgata ttttccagct ccacgcagga gacattctcg
     2161 ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat aaaaaaagcc ctcttcgtcg
     2221 cagtatccgg tatcgccggt atgtagccag ccgtcggctt ccagcacttt cgcagtggct
     2281 ttcgggttga gaaaatactc tttgaagatg gttttccctg gtacgccttt aatacagatt
     2341 tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt cgcggatctc cgcttcgtag
     2401 caaaaccccg cccgaccaat cgacggccag cgtcgtttat cgccagggcg atcgccgata
     2461 atgcccacaa tggtttccgt catcccataa gacgtcagca agcgaacgcc gaagcgttca
     2521 caaaacgcat ctttttcctg ctccgacaag ttgagataaa acatcacttc ccgcaggcgg
     2581 tgttgccgat cgttcgctga aggcggctgc gccatcaagg tacggatcat catcggaata
     2641 cattcggtaa cggtggcgcg gtacttctgt acctgtcccc agaaggcgcg ggcgctgtat
     2701 ttctcgacca gcacaaaggt ggccccggca gaaaacgccg ccatcgccgc agtacactgg
     2761 caatcgatat gaaacgcagg cattaccgtc aggtagacgt catcgtcacg cagtgcacac
     2821 tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat gggtaatcac cacacctttc
     2881 ggtcgggagg tggtgccgga ggtgaagaga atttccgccg tatcgtcagt cgatagcggc
     2941 ggtgcatagc acaaggtggc aggttgttga tttttcagtt gagtaaacga actcacgcca
     3001 tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc gcaattgagt ggcatcttcc
     3061 tgctgaatct gttgatacat aggatagaat tgcgcactgg tcaccagcag gcacgcctgg
     3121 ctattttgca ggatccacgc gctttcctcg cgcaacaggc gggcgttaat cggcaccata
     3181 atcgcgccaa tttttgccag cccgaaccag caaaagataa attccgggca gttgtcgaga
     3241 tgtagagcaa ccttgtcgcc tttgcgaatc cccagcgtat aaaacaggtt tgtcgtgcgg
     3301 ttaatctcct gatttaactc aagataacta taccggttaa cgactccgcc gctggattca
     3361 caaatcagcg ccgttttatg accgtaaacg tccgccagat cgtcccacat ttgacgtaga
     3421 tgttgtccgc caatgatatc cattgcacct ctatccattt ttgttcgttt gttattgggc
     3481 gggcgctagt caggcaagcc gactgacgcc acgcgtttag tcctcaactt tggc
//
LOCUS       DAJVYM010000239         3507 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVYM010000239 DAJVYM010000000
VERSION     DAJVYM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.244"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1523)
                     /locus_tag="OQS60_003847"
     CDS             complement(<1..1523)
                     /locus_tag="OQS60_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS2389475.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYL"
     gene            complement(1520..1819)
                     /locus_tag="OQS60_003848"
     CDS             complement(1520..1819)
                     /locus_tag="OQS60_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS2389476.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1826..2146)
                     /locus_tag="OQS60_003849"
     CDS             complement(1826..2146)
                     /locus_tag="OQS60_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389477.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2139..2336)
                     /locus_tag="OQS60_003850"
     CDS             complement(2139..2336)
                     /locus_tag="OQS60_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS2389478.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2390..2842
                     /locus_tag="OQS60_003851"
     CDS             2390..2842
                     /locus_tag="OQS60_003851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389479.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2900..3076
                     /locus_tag="OQS60_003852"
     CDS             2900..3076
                     /locus_tag="OQS60_003852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389480.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3188..3397)
                     /locus_tag="OQS60_003853"
     CDS             complement(3188..3397)
                     /locus_tag="OQS60_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS2389481.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          881 a          939 c          744 g          943 t
ORIGIN      
        1 atgagatagc cgcagaagtc caccagcgaa tcgccctcgc gttttatcac cagagcttct
       61 tctgatttct gctgctcata cagcaggcgt ttagcttcgt cctggtcaga aaaacgagta
      121 agcaggtggc gaatcacaac cgccagctct ccttctattt tttctgccag catggggtcg
      181 cgttcgttct ccggtacaac ttccgaaaaa ttgaatatca cccgacgacg tgagatcccc
      241 ccgctgcggt cactgaatga catggcgtta ttgttaaccg ccagcactac tgccggaata
      301 cgcgttgagt agggggcttt gtgtttcggg tcaattgcca ccttgtcacc gcctgtaatg
      361 gccttaatcc ctgccccatc accagcgtag cgggtcatat ccggcatgat aatcagcgaa
      421 aagccaacca ctaacgcgcg ttcccttgca tcttccagcg ccttcatgct tgccgatact
      481 gtgttggcct tacccgccag catggtgcaa atctccgcca tcacgctttt accacttccc
      541 cctggacctg ttacctcaat gaataactgc cagtcgtacc ggttcgccag caccatgaat
      601 aatacagcca gtacgcgatc cgccttgcgg tcattctcag ccaccgaacg gcgtaaccac
      661 ttccagaaat tcggcgcatg tgttgccagc gtttccccct ctgctggtgg gctgaaaggt
      721 aattcactgg caattaacaa ccagtcgttt ttgttatgct cccgaaaatt acctgttctg
      781 gtatcaaata ccccgttact gaatccaatc aggttacggg ctgtattccc cattacaggc
      841 aaacttaact tcatggtatc gaccgccgat ttaatggcgt tctgcgaata gctgatctcc
      901 gcatcaatga aaatctgtgc catagctcgc tgtaattctt tatcctgtac tggctcccat
      961 acaacgccgt tgtaatggtg aacagtgtca gagtcagcat gaatcgccag ttcaccgcca
     1021 taatgtgcca ggagaacttc gccgcgttga cttgctccca tctggttaag cgccagtgat
     1081 gaagcgttat cgtcttttac ccgctctttt ttctttacag gcagttcaac tacctttttc
     1141 ttttccgcct gctctgcccg ttcacgttcc agatattcgc gccagttctc ccgtttctgg
     1201 ctgtgcattc cttcagggta ataatcagca tccctgacac ctgccgctgc cagtttctgc
     1261 ccgatggtat taacaagccc cggacgcaat aaccccgcct ggtagagacg cacccgatag
     1321 cgtccgtccg gtacgatttg cagcttatcc agttcggcaa gttgttgttc tccaagccag
     1381 acaggaggca cgctgtcgcc agccagtcgc ccgtcctgtt cctgccactg cttcgcatgt
     1441 gcccacgcat cattacccgc aaaaatgatg acttccgtca ttttgtcacg cggctggtgt
     1501 tttaaatttg gcgctttttt catttctgct ctctccacgc ggcaatcatg tttttcagtt
     1561 cctgtagttt tttatcaaca tccatacatg acacatggtt atttctggaa agcgggattt
     1621 cccgcctgaa tctgctaata aagatctcca cgttcagcga actatgaaat gaatagccat
     1681 cacgaataaa atacacacgg tcaaacatca gttcttttac cgttactctg ttaccgttct
     1741 tatccagata aatagcgccg gggataattt tggggtgtgc ataaccgctg gcagtcaagc
     1801 cagataaata cgttctcatg attatttatc tcagatttga atcagtattc gctttctcta
     1861 tggcatttaa tgcatctgtg gcattttcaa tggtgcaccg taacgaaata tcaaactgcc
     1921 caagcattgc cagtaacaag ccaatattac ccatgtcaat gcgcatagcc ttttcgtcat
     1981 attcctcatt ttctgacgca tgccacatca ggctaccaat tgacgcaaca gccattgata
     2041 tattgtcagt agccccatcc gcagcggaat aaaccttttt agcaatatca tgctcacagt
     2101 taaaatgcgg attaatcagg tactggtaat tggtcatgtc aggcatggca cacctcctga
     2161 cgaatacggg cggcgaatac agcaacacaa cctgacgggc aacggttacg cgcttcgcgt
     2221 tccgtccagg cggttacgtg gatgatttga gattctccgg cactcagggc cagaaaacgc
     2281 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
     2341 gctcctcgtt tacgttgttg gttagaagcc ctgcgagtgg taacgacact tgcggggctt
     2401 cgtcgtttca attgctgaat tgcatgtatc agcacttgtg gtattcacat tacatttagg
     2461 tgaataccat ttcaagtctt ttttggtatt cacttttgta ttacactgca tcccgttatt
     2521 aatgggaggt gcaaacatgt ccaggagttc tgttaacaat aagtcacagc aactgaatgc
     2581 cagatttcca catgaagtag tgagtggcat tgaggcatcc ctacagccag gggaaactaa
     2641 agcgaatttt atagttacgg ctgtacgcgg tgagatcgcc aggcgccagg cagaaggaag
     2701 tggagaaaat ccccttgtgt cttcactgga tgccctagct aatgtcgaac aaatcggcat
     2761 caaggcagcg gaggaaatcg ggcagcttat caccgttgca cgcgaagaac tccagcgccg
     2821 caaggccaaa gaatcagaat aatcactatc agcgccgtgg tgtgaggaac tccggcgcat
     2881 tgctttacag gcgcacacaa tgaccaacga agaatcaacc aaaaaaacat cacctgcacg
     2941 aaaaagacga cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc
     3001 ccttgagaag taccttaaag aatactcatc aaaggagaga gcttcgcagt tatggcaacg
     3061 cactgaaaca gactgatagc attgcccacc agcctgatag cggctatcat tgacatgctt
     3121 atgtttagtg tttccaaact ggcgaccgcc ccaggtcgcc tttgttttat gtgtcatatg
     3181 ctccccttta cactgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa
     3241 cctcggacag cagccaccct acagaacgac cgcccagatt aagacgtgac ggaaagcgcc
     3301 cttttttctc cagttcgtaa cgtgtagtgc ggcacactcc agttaactta cgacattcat
     3361 cctcacggat tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac
     3421 gtatataaac ttttctctta gctgtagata tatgagatcg gatattactt agattctttt
     3481 tcacctctta aattaaaaac acaacca
//
LOCUS       DAJVYM010000240         3492 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVYM010000240 DAJVYM010000000
VERSION     DAJVYM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.245"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..604
                     /gene="dcuC"
                     /locus_tag="OQS60_003854"
     CDS             <1..604
                     /gene="dcuC"
                     /locus_tag="OQS60_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS2389482.1"
                     /translation="GKWGPQLHIITILVICMLIASILEFLRSFNTQKVFSGLEVAYRG
                     MADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFGSASIILMLVLVILTMLAAV
                     TTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASNLGRTLSPVSGVVVAVAGMA
                     KISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTGK"
     gene            complement(645..1325)
                     /gene="dpiA"
                     /locus_tag="OQS60_003855"
                     /gene_synonym="citB"
     CDS             complement(645..1325)
                     /gene="dpiA"
                     /locus_tag="OQS60_003855"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS2389483.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(1294..2952)
                     /gene="dpiB"
                     /locus_tag="OQS60_003856"
     CDS             complement(1294..2952)
                     /gene="dpiB"
                     /locus_tag="OQS60_003856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCS2389484.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            3332..>3492
                     /locus_tag="OQS60_003857"
     CDS             3332..>3492
                     /locus_tag="OQS60_003857"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS2389485.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGG"
BASE COUNT          897 a          893 c          787 g          915 t
ORIGIN      
        1 cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc
       61 ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt
      121 ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg
      181 ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac
      241 ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga caatgctggc
      301 ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa
      361 actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc
      421 aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg ccgggatggc
      481 gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg ttggtttggt
      541 gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacaggcaa
      601 gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat
      661 atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg
      721 gcgcaatatt caagataacg cctggcagtg gtgcagctga tggtcagtgc ctgcgccacc
      781 gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc
      841 aaggggtcaa tgccggtcgg tagctcgtcc ttaggttcac cgcgagcata agcattaaac
      901 atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg
      961 cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat gagataatca
     1021 aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc agtggtaaac
     1081 accacgtcgc cgggataatg cgcctgcacc agttcatgca gtaaattaat ccctctaccg
     1141 tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat catcattcgg
     1201 gcctgcgcca gatttcccgc cagtaatatc tgactgaatc cgggaatgtg acgaatatat
     1261 tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt taatggagct
     1321 gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg gatcattatc
     1381 ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt acaacccaat
     1441 gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata ttttgtctct
     1501 tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat catcgccttc
     1561 atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc tggcttcgaa
     1621 ggcgttatca agtaaattgc caacaatggc ggcaaactcg gtgctatcca gtcctggcgg
     1681 cagttgcgaa agctggctac cggggacgat gatcattttt agccccagtt cgcgggcgcg
     1741 ctgcacttta ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct cacgcaggct
     1801 gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca cgcgatcata
     1861 ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgtgatgct cgtgtcgcaa
     1921 tgtacgaagg ctctcgacgt attgttttat ttgcgtcagt tgcgcattga gggtggaaat
     1981 ttcgtcttta ctacgaaagc tgataatggc ccccagcaaa tcatcacctg aacgaatagc
     2041 ttcccggttg gcaataacgc tcagaccatt aaagttcgcc accacatcct gacgtttttc
     2101 atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg gtttacccaa
     2161 ccattgccgt ccgggagagc tcagccccag catctttctt gcgttacgat tgatggcggt
     2221 aatgtaacca tgcggatcca ccgcaatcag cccttcataa accgaactaa acagcgcctc
     2281 ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcattt gtcgacggat
     2341 atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga caaacacgcc
     2401 tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca ggtagccaat
     2461 cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg cgcgcatcgc
     2521 catccccatt gcccctttcc cggtaatgaa gtagctctcc cctttctcca gcgcgccctg
     2581 tttggtgaac tgcataggat aaccaatttt ctccggatta agatggtaaa ggcggatcga
     2641 gtgccggtcg ccaatcacca cataatcaaa atcggtatct ctttgtaatt tgttagcgat
     2701 ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag atgacactgt cattggaggc
     2761 aataattttc gcctgattca ttgccatgtc gcgtacatga agcgttaaat agtcctcaaa
     2821 actggccgta aaatattgcg ccagggctgc aatgacaaat attgagaaca ccagaatcag
     2881 caaaaagata cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt tattctcgtt
     2941 aagctgcaac attgttttcc ttgcgggttg tcccgagcct ctttgacgga tgtaattaaa
     3001 atgttgaatc aaaatacata gatattaatt atgatcgtgg caaattttat tcattaaatc
     3061 aattaaatca cttaaacctg caggtcattt cgtaattaca ttaattaaaa aacttaaaac
     3121 cattaaataa ataaaaaacc actaactcta tgtgaaataa atcaaaattt cacgctgcaa
     3181 tactccttag aatgtatagc gaaaggagaa aaagatatac ctcgatcacc cccctttctc
     3241 ccaagtgaaa ataaaaggtt atcaacttgc aacattgaac aacattcgtt gcaaatcgat
     3301 aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt tcacccgcgt
     3361 aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt
     3421 gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa agcttatcgc
     3481 gtgcggtgga at
//
LOCUS       DAJVYM010000241         3375 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVYM010000241 DAJVYM010000000
VERSION     DAJVYM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.246"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            6..326
                     /locus_tag="OQS60_003858"
     CDS             6..326
                     /locus_tag="OQS60_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCS2389486.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OQS60_003859"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OQS60_003859"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS2389487.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OQS60_003860"
     CDS             2213..2929
                     /locus_tag="OQS60_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2389488.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          933 a          738 c          737 g          967 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaact
//
LOCUS       DAJVYM010000242         3286 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVYM010000242 DAJVYM010000000
VERSION     DAJVYM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.247"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..230)
                     /locus_tag="OQS60_003861"
     CDS             complement(<1..230)
                     /locus_tag="OQS60_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389489.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            404..1117
                     /locus_tag="OQS60_003862"
     CDS             404..1117
                     /locus_tag="OQS60_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS2389490.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1149..1907
                     /locus_tag="OQS60_003863"
     CDS             1149..1907
                     /locus_tag="OQS60_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389491.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1953..>3286
                     /locus_tag="OQS60_003864"
     CDS             1953..>3286
                     /locus_tag="OQS60_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS2389492.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          751 a          790 c          854 g          891 t
ORIGIN      
        1 aatttttttg ctgttttcac ctgatttcgt tcaattgttt tatgtaagga ttttcccatc
       61 aacggtaatt ttgtcactgc acgaccgaca tttccggcct gttttcctaa atgaactctt
      121 tcggcagcac catatttttc gacaacggta ataagatgtt cacacaccgt tgacttgcct
      181 gaaccatcgc taccaataac ggcaattaat ggaggtgtta ttgactgcat gaatatatcc
      241 tttaatttaa acatccatta aaaatattta tttggttaat atgtttttat gaaagcgtaa
      301 ttcaggtcaa tgtcacaatt aaccattgtc acaataaggt tgaacggata ttctgagcac
      361 ggaccacgta atattcaata cattatttac tgccgggttt ttcgtgaatc aacgtaatat
      421 gtcaataatt aattccacgc cagtgcgtgt tattgccatt gtaggatgtg atggttcagg
      481 taaatcgccc ctcacggcaa gcctggtaaa tgaactggca gcaagaatgc caacagaaca
      541 catttatctc gggcaatcgt ccgggctaat tggcgaatgg atttcacagc tccctgttat
      601 tggcgcacct tttgggcgtt atctgcgaag taaagcggca catgtgcacg aaaaaccctc
      661 aacaccgcct ggcaatatta ctgctctggt tatctatctg ctttcctgct ggcgggcgta
      721 caagtttcgc aaaatgttgt gtaaaagcca gcaaggcttt ctgctcatca ccgaccgcta
      781 cccgcaggtt gaagtgccgg gttttcgctt tgatggcccg caattggcaa aaaccacggg
      841 cggtaacggt tggataaaaa tgttaaggca gcgcgagctg aagctgtacc aatggatggc
      901 atcttatttg cccgtattgt tgattcgtct tggcattgat gaacaaaccg cgtttgcgcg
      961 taaacctgac caccaactgg cagcgttaca ggaaaaaatc gccgttacgc cgcaactgac
     1021 tttcaacggc gccaggatcc ttgaactgga tggtcggcaa ccagccgatg aaatcctgca
     1081 agcgtcacta cgtgcaattc acgccgcgct ttcctgatca ccaaaatgga taggggatga
     1141 tttaacgaat ggatgcacta caaacacaaa ctgttaatag tacaaccgcg ccgcagccca
     1201 gctacattcc ggggctaatt gcggtggtcg ggtgcgatgg caccggtaaa tccacgctga
     1261 ccaccgatct ggtgaaatca ttgcaacagc actggcaaac cgagcggcgc tatctggggc
     1321 tgctctcggg cgaagacggc gacaaaatca aacgattacc gttggttggc gtctggctgg
     1381 aacggcgact ggcggccaaa tcctcgaaaa cccaaagcat gaaaaccaaa tctccggcgc
     1441 tatgggcggc ggtgattatg tactgcttat cgctgcgaag aatggcgaat ctacgcaagg
     1501 ttcagcgact ggcgcaaagt ggcgttctgg tggtcagcga tcgcttcccg caggctgaaa
     1561 tttcgggctt ttattacgac ggaccgggga ttggcgtcga acgtgcgacc gggaaaatca
     1621 gcatgtttct ggcgcagcgc gaacggcgtt tataccaaca aatggcgcaa tatcgcccgg
     1681 aattaattat tcgcctgggc attgatattg agactgccat ctcccgcaag cctgaccatg
     1741 actatgccga gctgcaggac aaaatcggtg tcatgtcgaa gattggctat aacggcacaa
     1801 aaattcttga gattgattcc agagcgccct acagcgaagt gctggaacag gcacagaagg
     1861 cggtttctct ggttgccatc gtttctgacc gccgaagttt aacttaaggc tggcgggata
     1921 ctttttacta tcaacgataa ggtcaggctg aattggcggg ttttaacatc aaacattggt
     1981 ttgcagatgg cgcgtttcgc accattattc gcaatagcgc ctggttaggc tccagtaatg
     2041 tcgtgagcgc cttgctgggg ctgttggcgc tctcgtgtgc cggtaaaggg atgacgcccg
     2101 ccatgtttgg cgtactggtg attgtgcaat cgtacgccaa gtcgatcagc gattttatta
     2161 agtttcagac atggcagctg gtggttcagt acggaacgcc agccttgacc aacaataatc
     2221 cgcagcaatt tcgcaatgtc gtctcatttt ccttctcgct ggatatcgtc agcggcgcgg
     2281 tggcgattgt cggcggcatt gccttactgc cattcctttc ccattcatta ggtctggatg
     2341 accaaagctt ttggctggcg gcgctgtatt gcacgctcat tccttcaatg gcttcctcca
     2401 cgccgaccgg cattctgcgt gcggtagatc gcttcgattt aattgctgta cagcaggcga
     2461 cgaaaccttt tctgcgcgca gcggggagcg tcgtagcctg gtattttgac tttggttttg
     2521 cgggttttgt tattgcctgg tacgtgtcga atctggttgg cggcaccatg tactggtggt
     2581 ttgcagcgcg cgaattacgc cgcagaaata tccataatgc cttcaaattg aatctgtttg
     2641 agtctgcccg acacatcaaa ggcgcgtgga gttttgtctg gtcaaccaac attgcccact
     2701 ccatctggtc ggcgcgtaac tcgtgcagca cagtgctggt ggggatcgtg ttaggacccg
     2761 ctgccgccgg gttatttaaa atcgccatga cattctttga cgccgccgga acgccagcgg
     2821 gtttgctggg taaaagtttt tacccagagg tgatgcgttt agatccgcgc accaccagac
     2881 cgtggttgtt aggtgtgaag tccggtttac tggcgggcgg aatcggtatt ctggtggcgc
     2941 tcgcggtgtt gatcgccggc aagccgctca tttcgctggt gtttggcgtt aagtatctcg
     3001 aagcgtatga cctgattcag gtgatgctgg gcgcaattgt gatttcgatg ctgggtttcc
     3061 cgcaggagtc attgctattg atggcgggaa aacagcgcgc ttttctcgtg gcgcaaacca
     3121 tcgcctcaat cggctacatc gtattgctgt tcatgttctg tcatctgttt ggtgtgctgg
     3181 gagcagcgtt tgcttacttc ggtgggcaat gtctggatgt ggtgctctcg ttaattccaa
     3241 ctttaaaggc attttttcag cgtcattcct tgctttataa cgcagc
//
LOCUS       DAJVYM010000243         3254 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVYM010000243 DAJVYM010000000
VERSION     DAJVYM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.248"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(108..635)
                     /gene="mobB"
                     /locus_tag="OQS60_003865"
     CDS             complement(108..635)
                     /gene="mobB"
                     /locus_tag="OQS60_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS2389493.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(617..1201)
                     /gene="mobA"
                     /locus_tag="OQS60_003866"
     CDS             complement(617..1201)
                     /gene="mobA"
                     /locus_tag="OQS60_003866"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS2389494.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1271..1540
                     /locus_tag="OQS60_003867"
     CDS             1271..1540
                     /locus_tag="OQS60_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS2389495.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1617..2603
                     /gene="srkA"
                     /locus_tag="OQS60_003868"
     CDS             1617..2603
                     /gene="srkA"
                     /locus_tag="OQS60_003868"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS2389496.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2620..3246
                     /gene="dsbA"
                     /locus_tag="OQS60_003869"
     CDS             2620..3246
                     /gene="dsbA"
                     /locus_tag="OQS60_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS2389497.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          834 a          784 c          790 g          846 t
ORIGIN      
        1 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcatt ggcgcagaaa
       61 aaaatgccgg atgcgacact ggcgcgtctt atccagccta ctcttgctta tccgttctgc
      121 ttttgcatcc actccaccac aaaatcagcc agcccctcaa catcattaat atccagtaat
      181 gcgacatcaa gattaagcgg cacatcactg gctacagcaa taacatgcct gtctatcact
      241 aattcttcag gtcgatgtcc ggctccatcg cgaaacagca caatctttgc gatctcttca
      301 tgcttaaacc cttcgaccag aatcaaatcc agctttgagg tatccattcg acttgcgaga
      361 aactgtagat ccagctcttc ttcgtctggt ttttccgtca tcaaggccca tcgttgctgg
      421 ctggcaacga tggtttgtgc cgcgccagcc ttgcgcagct catagctatc tttgcctggc
      481 ttatcaacat ccatatcatg gtgcgtatgc ttaatcagcc ctggacggat ccctctggcg
      541 cataatgccg ggatcagttt tttcaaaagc gtagtttttc cggtaccact ccacgcggca
      601 aaggcgagta acggtatcat cgtttttcct gccatcgggc aagctcctct ggcgtattca
      661 cgttaacaaa tgcatcttta tgatcgctga aatcaaccgc atggccgcca gccagacgca
      721 taaatgccat tacccggcgt tctcctgctt gcagatattc tagtaataaa ggctcaatag
      781 cacggtttac cagagcaata gtcgggtgat cgcgttcacc gtcatggacc cacacgacag
      841 gcgcatcttt gcgctgatga ttaagccggg aagctaaaac atggggaatg taaggcgtat
      901 cgcacggaca aaacaaaaac cactcaccag cttcctgctg cattactgaa agcattcctg
      961 ccagagggcc tgggtaatcc gccagtgaat cttcaatcac tttcagaccg cttgcctgat
     1021 agatttcctg atgacgattg gcattaacca cgacgtgaga gagctgcgtc ataagcgcgt
     1081 cagcgacatg ttgccataat ggtttgccgt ttaattcaag caatccttta tctacgccgc
     1141 ccattcgtct ggctttaccg cctgccagca caacgcctgt tatcgtcgtc atcagattca
     1201 ccgatatcgc ctcttttatt gtgggattga ccctgctaac gtgtctgtct caagagtaag
     1261 gagcatcact atgaaatgta aacgtctgaa tgaagttatt gaactcctcc agccagcctg
     1321 gcaaaaagag ccagacctta acctgttgca atttttgcag aaactggcga aagagtcagg
     1381 ttttgacggc gaactggcgg atttgacgga tgacattctg atctatcacc tgaaaatgcg
     1441 tgattccgcg aaagatgcgg tgatcccggg tttgcagaaa gattatgaag aagacttcaa
     1501 aacggcgctg ttacgcgctc gcggcgtaat taaagagtaa aagcttgtaa gcggcgccac
     1561 caaaatcatc gtgaaatgat atccttcatc attcgtaatg ttttccggat gatgggatga
     1621 acaacagcgc ttttactttc cagacactac acccggatac catcatggac gctctgtttg
     1681 agcaagggat ccgggtggat tccggtctta ccccgcttaa cagctatgaa aaccgtgtct
     1741 atcaatttca ggacgaagag cgtcgacgtt ttgtcgtcaa attttatcgc cctgaacgtt
     1801 ggacagccga tcaaatcctc gaagaacatc aatttgcgtt gcagctggta aatgatgaag
     1861 ttccggtcgc agcacctgtg gcctttaacg gtcagacttt attgaatcat cagggatttt
     1921 atttcgctgt ttttccaagc gtcggtggtc gccagttcga agctgataat atcgatcaga
     1981 cggaagcggt tgggcgttat ttagggcgta tgcaccagac ggggcacaaa cagcttttta
     2041 tccatcgccc gaccatcggt ctgaatgaat atctcattga gccacgcaag ctgtttgagg
     2101 acgctacact gataccttcc gggttgaaag cggcattcct gaaagcgaca gatgagctga
     2161 tcgccgccgt tacagcacac tggcgggaag atttcaccgt tctgcggcta catggagact
     2221 gccacgccgg gaatattctc tggcgcgatg gtccaatgtt tgttgatctg gatgatgcac
     2281 gtaatggtcc agctattcag gatttgtgga tgttgctcaa tggcgataaa gccgagcagc
     2341 ggatgcaact ggaaactatt attgaagctt atgaagaatt tggcgagttc gacaccgctg
     2401 aaatcggact gattgaacct ttacgcgcca tgcgtttggt ttattatctt gcctggctaa
     2461 tgcggcgttg ggctgatccc gcgttcccga aaaatttccc gtggttaacc ggggaagatt
     2521 actggctgcg acaaacggcg acttttatag aacaggcaaa agttctacaa gaaccccctt
     2581 tacaattaac acctatgtat taatcggaga gagtagatca tgaaaaagat ttggctggcg
     2641 ctggctggtt tagttttagc gtttagcgca tcggcggcgc agtatgaaga tggtaaacag
     2701 tacactaccc tggaaaaacc ggtagctggc gcgccgcaag tgctggagtt tttctctttc
     2761 ttctgtccgc actgctatca gtttgaagaa gttctgcata tttctgataa tgtgaagaaa
     2821 aaactgccgg aaggcgtgaa gatgactaaa taccacgtca acttcatggg tggtgacctg
     2881 ggcaaagatc tgactcaggc atgggctgtg gcgatggcgc tgggcgtgga agacaaagtc
     2941 actgttccgc tgtttgaagg cgtacagaaa acccagacca ttcgttcagc atctgatatc
     3001 cgcgatgtat ttatcaacgc aggtattaaa ggtgaagagt acgacgcggc gtggaacagc
     3061 ttcgtggtca aatctctggt cgctcagcag gaaaaagctg cagctgacgt gcaattgcgt
     3121 ggcgttccgg cgatgtttgt taacggtaaa tatcagctga atccgcaggg tatggatacc
     3181 agcaatatgg atgtttttgt tcagcagtat gctgatacag tgaaatatct gtccgagaaa
     3241 aaataattca atgt
//
LOCUS       DAJVYM010000244         3233 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVYM010000244 DAJVYM010000000
VERSION     DAJVYM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.249"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1437)
                     /locus_tag="OQS60_003870"
     CDS             complement(<1..1437)
                     /locus_tag="OQS60_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS2389498.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYI"
     gene            complement(1522..1857)
                     /locus_tag="OQS60_003871"
     CDS             complement(1522..1857)
                     /locus_tag="OQS60_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS2389499.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(1857..2213)
                     /locus_tag="OQS60_003872"
     CDS             complement(1857..2213)
                     /locus_tag="OQS60_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS2389500.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2213..>3233)
                     /locus_tag="OQS60_003873"
     CDS             complement(2213..>3233)
                     /locus_tag="OQS60_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS2389501.1"
                     /translation="AGVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVG
                     AAGSGAPDLTAAVAAMGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHG
                     YTAKLGTLSELVGAGDMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARP
                     TQTGELVGMLPAPKGKRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVA
                     DNSYLDSETLHTSAYVLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLA
                     TYRQMERAGIVENYDLFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQY
                     SEESA"
BASE COUNT          728 a          976 c          724 g          805 t
ORIGIN      
        1 aatatacagg gcagaaaccg ttgctattgt ttttactgcc tgttcccatc cccccattgc
       61 tctgacaacc tgatcaacct cctgccagac ttttttcaca accggaccga ccgtttccca
      121 gttatcaatt atgagatatg caccaccgac gagaagcgca attaatccct ttgccggcgt
      181 catattcatc acgctaccca tgatttttgt tatgcgtgtc aatgtgccaa tcgccacgcc
      241 catcgtcagt aatgccgcac cagttttcgc gatggttttt acgacctcag gattttctct
      301 gacaaacgtg cgcacttctt ccagaaaagg cttcatctct tttatgcctt cattgagtga
      361 aggcaggaag gtttccccca gcgtggaaga aatcgcattg atctggttct gcagcagcaa
      421 caactgattt tctgtcgtcg cggcacgggc ggcatattct ttctgcattg agccgccata
      481 ctgctgcgca tccgccacac gattaaagtt ggttcgcaac aaatccagat tcgtgagcag
      541 cggcgcaatg gcccccaggg attccttccc gaacagcgct ttcagcacag cagcctgttt
      601 ttctttcggc acttttgcca gagaatccag tacgtgcagc atggccccgc gggcgtcttt
      661 ctgcatatcc gccgccagtt ttttcgggct aatgcgcaac gagcggagca cttttttctg
      721 cgatttggtg gcagaatccc tcgctgtcag cgacaacata aaattcttta tccccgtcgc
      781 agcaatttct gactccaccc ccattccggc aatggttgcc cccattgcgg caatctctcc
      841 ggaggccaca cccgcaacac tgcctaaagg gccaatacgg gtcacaacat cagaaatctt
      901 tttcgcactt gcaggaccgg tattaccaag atagttaatc ttatccgcaa gccctgccac
      961 ctctccctgt gtcagtttaa aggcagtgcg ccactgcgcc atcatctgac cggattcttc
     1021 tgccgtggtg tcaaaggcca cgcccatctt cacggcatcg tcagtaaact gcatcagctc
     1081 gtcacgggcg ataccagcct gaccacccgc cgccacaatt tcggcgatac cttccgccga
     1141 catgggcagt tctgttgaca ggtcgcgcac ctgctccgtc attgccttaa acgcttccgg
     1201 cgtatccaga ccatccacca ctttccggac atccgccatt tttgattcaa gggcaatggc
     1261 agatttgacc gggagcgcca gcgcccccag tactgcggtt ccggcacccg ctgcgcccag
     1321 taacaggctg gcaaactcct ttttaaaacc tttcagctgg cgctgcattc ccttcagcgg
     1381 tgccgatacc ttatccacgg cagtgatgat cgccttcagc tgaaaactgt cagccatgct
     1441 tcatctcctc gttaatgcgg acggcctcgg cttccagttc tgtaaactgc gaaacagccc
     1501 cccgtcgcag ctccagtgga ttcagtttcc agaaccaggc aacattgtag agtcgtttcc
     1561 ggaggtgctt cccgtctccg actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa
     1621 aatatccagt tttgccatct gcgccgccga tgaacgcggg atccccgcca gcaacgggat
     1681 atatttcagt gccacctggc tgtccagttt aatactgcct tcaccggaaa taatgaaagg
     1741 aaaacccagc gcctcgattt cgtcatacgt gggctcgcgc aactccagca catgcagcat
     1801 ttcgttatgc gctgtcaccg gtttttttaa aactatttct gtaacgttca ttgccattac
     1861 tgataccctc cctcttcgcc gtggaattca agatctgccg tgccttcttc ggcattatga
     1921 ttagcctctc cgtgcagcca tgccgccgaa agcacataca ccatgccgtt tgccagctca
     1981 gcggtaatcg tcatctggtc tgaggtggta attttgtcga ccggaaagtt tttgggcact
     2041 ttaaacgtgc ccttaacgta aggtgaccgc caggtctcct tgtaatccac atcccctgcc
     2101 atgccgacga tgtcatcccg gacattggtg ttcatcggca cttcaatgcc acctgtcagc
     2161 gataactgct gaccatccac tttaaaaaaa caggttccgg cgatctttgc cattatgctg
     2221 actcctctga atactgaaga cggaactggt taaccaccgc aaagacacgt aactgattaa
     2281 cataatccgg cgggaacagc gtattcagtc ggttcgggtt atctgcatca cgctcaacta
     2341 tcagatactg tttaaacaga tcgtaatttt ccacaatacc ggcacgctcc atctgacgat
     2401 atgtcgccag aagttcccct ttgataacgg caggagtgac aatcgcctgc cccggaccaa
     2461 aacgggtacc atcatttgcc agcttgtgac gtccgtactt gcttgtgatg accgatttca
     2521 gtttgcgaag aacatatgca ctggtatgca gagtttcact gtccagatag ctgttgtctg
     2581 ccacgccata cgcattcttt ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc
     2641 cttccacata agccgtcgcc acgccgtgag ataaaagggt ctgctgctct gtcatgatga
     2701 atcgcttacc tttcggtgcc ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg
     2761 caggatcatt ccggataaat accgcttcac gggcaaggcg actggcaacc agttcatcga
     2821 caggcgactg ggtttctttt tcgtaaccgg caagcgtgat atgttgctga ttatgcatat
     2881 ctccggcacc aaccagctct gacagcgttc ccagttttgc ggtatagcca tgcccgtata
     2941 actggcgcgc ataactccag cgaccgctgc tgtcattcat ttcggtcatc atcatattga
     3001 tggaggcggc atcgttgaac ggcagaccga taaagtcgaa tgcctcatcg cccatagcag
     3061 caacagcggc ggtaagatca ggcgctccgc taccggcagc accgacttcc gtcaccaccc
     3121 gaagccctgc tggcagaatc tcaccaccac cagaaccata ataattcaga cagaccggta
     3181 actcattacc atacagcccc ttatgacggg cagtcagcgt caccacacct gca
//
LOCUS       DAJVYM010000245         3203 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVYM010000245 DAJVYM010000000
VERSION     DAJVYM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.250"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..513)
                     /locus_tag="OQS60_003874"
     CDS             complement(<1..513)
                     /locus_tag="OQS60_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS2389502.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OQS60_003875"
     CDS             complement(759..1211)
                     /locus_tag="OQS60_003875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2389503.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQS60_003876"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQS60_003876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS2389504.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQS60_003877"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQS60_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS2389505.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3100..3203
                     /locus_tag="OQS60_003878"
                     /pseudo
     CDS             3100..3203
                     /locus_tag="OQS60_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          765 a          829 c          794 g          815 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cga
//
LOCUS       DAJVYM010000246         3152 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVYM010000246 DAJVYM010000000
VERSION     DAJVYM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.251"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..261)
                     /locus_tag="OQS60_003879"
     CDS             complement(<1..261)
                     /locus_tag="OQS60_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCS2389506.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSL"
     gene            381..839
                     /locus_tag="OQS60_003880"
     CDS             381..839
                     /locus_tag="OQS60_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS2389507.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1022..1546
                     /gene="aroL"
                     /locus_tag="OQS60_003881"
     CDS             1022..1546
                     /gene="aroL"
                     /locus_tag="OQS60_003881"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS2389508.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            1596..1787
                     /gene="yaiA"
                     /locus_tag="OQS60_003882"
     CDS             1596..1787
                     /gene="yaiA"
                     /locus_tag="OQS60_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS2389509.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2045..2722
                     /locus_tag="OQS60_003883"
     CDS             2045..2722
                     /locus_tag="OQS60_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS2389510.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            2794..3078
                     /gene="ppnP"
                     /locus_tag="OQS60_003884"
     CDS             2794..3078
                     /gene="ppnP"
                     /locus_tag="OQS60_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS2389511.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          811 a          719 c          814 g          808 t
ORIGIN      
        1 caggctggag gtgatttcgc taagcacttt aatcatgatg ccaggtttaa cggcggcaaa
       61 aatgatgtcg gcgatttgcg ccacttcttg cgccgattct gcggcgttga tgccgaactg
      121 gtcatgcagg gcggcgactt tatccgggga gggggtgtat acccagattt gccctggaag
      181 cacctgaccg ctggcaatca gaccgccgag aatggctttt cccatattgc cgcagccaat
      241 aaaaccgatt ttcttttcca ttgcctcact cctgccgtga aattcattgt tttgataatc
      301 gctggcagaa gcataaacag aactatgccg gaaggcaaaa gcgcgacaca atagaggatt
      361 acccaacaaa ggatgacttt atgacaattt gggtggatgc cgacgcgtgt cccaatgtaa
      421 ttaaagagat tttgtatcgc gcggcggaac gtatgcagat gccgctggta ctggtagcaa
      481 accagagttt acgcgtgccg ccatcgcgat ttattcgtac gctgcgcgtc gcggcaggtt
      541 tcgacgttgc cgataacgaa attgtccggc agtgtgaagc gggcgatttg gtgatcaccg
      601 cagatatacc tttggctgct gaagccatcg agaaaggcgc agcggcgctt aatccgcgcg
      661 gcgaacgtta cacgccagcg accattcgtg agcgcctgac gatgcgcgat tttatggata
      721 ccttacgtgc cagtgggatc cagaccggcg gaccagatag cctttcacaa cgtgaccgcc
      781 aggcctttgc cgcggagctg gagaagtggt ggctggaagt gcaacgtagt cgtggctaaa
      841 tgtaatttat tatttacact tcattcttga atatttattg gtatagtaag ggatgtattg
      901 agattttcac tttaagtgga attttttctt tacaatcgaa attgtactag tttgatggta
      961 tgatcgctat tctcatgaca ccggctttcg ccgcattgcg acctattggg gaaaacccac
     1021 gatgacacaa cctctttttc tgatcgggcc tcggggctgt ggtaaaacaa cggtcggaat
     1081 ggcccttgcc gattcgctta accgtcggtt tgtcgatacc gatcagtggt tgcaatcaca
     1141 gctcaatatg acggtcgcgg agatcgtcga aagggaagag tgggcgggat ttcgcgccag
     1201 agaaacggcg gcgctggaag cggtaactgc gccatccacc gttatcgcta caggcggcgg
     1261 cattattctg acggaattta atcgtcactt catgcaaaat aacgggatcg tggtttattt
     1321 gtgtgcgcca gtatcagtcc tggttaaccg actgcaagct gcaccggaag aagatttacg
     1381 gccaacctta acgggaaaac cgctgagcga agaagttcag gaagtgctgg aagaacgcga
     1441 tgcgctatat cgcgaagttg cgcatattat catcgacgca acaaacgaac ccagccaggt
     1501 gatttctgaa attcgtagcg ccctggcaca gacgatcaat tgttgatttt agagcgccta
     1561 tacttaacgt tcatcccgtg aaataaggaa gaacgatgcc aacgaaaccg ccttatcctc
     1621 gtgaagcata tatagtgacg attgaaaaag gaaagccagg acagacggta acctggtacc
     1681 aactcagagc cgatcatcct aaaccagact cgttgatcag tgaacatccg acggctcagg
     1741 aagcgatgga tgcgaaaaaa cgctatgagg accctgacaa agagtgaccg catcagactg
     1801 ctcggaaggg attctgagtg ccactacaag ggatctgcgt cacatttttc ataattcatg
     1861 tttttctaat aattagaata ttaaacaata acaatccatt actggaatca tttggaatct
     1921 ttacattatg ccgtgcacgt ctgctgctac gctttttgtc atttgtagca caagtaagtg
     1981 tcagcagtgg tgctccacac ttgcccggta attaacgacg aaagaaaagt aaggtggatg
     2041 aacaatgagt gcgtcgttgg cgatcctgac catcggcatt gtacctatgc aggaggtttt
     2101 gccgctcctg acggaataca ttgacgaaga taatatttcc catcatagcc tgctggggaa
     2161 gttaagtcgt gaagaagtga tggcggagta cgcgccagaa gcaggcgaag acaccattct
     2221 cacattatta aatgacaacc agctggccca tgtttcgcgt cgtaaagtgg agcgagacct
     2281 gcaaggtgtg gttgaagtgc tcgataatca gggttatgac gtcattttat taatgagtac
     2341 agcaaacatt agtagtatga ctgcgcgtaa tacgatcttt cttgagccgt cgcgaatatt
     2401 gcctccactg gtttcctcta ttgttgaaga tcatcaggtg ggggttatcg ttccggttga
     2461 ggagatgctg accgttcagg cgcaaaaatg gcaaattttg cagaaatcgc cggtattttc
     2521 attgggtaac cccattcatg attcagaaca aaaaatcatt gatgccggga aagaattact
     2581 ggcaaaaggg gctgatgtca tcatgctgga ttgtttagga tttcaccaac gtcatcgcga
     2641 tttactgcaa aaacagctcg atgttcctgt cttgctgtct aacgtattga ttgcacggct
     2701 ggctgcggaa ttactggtgt aattttgcgt gacagccagc gcctctggcc cctatagtga
     2761 agtagatgtt caactaccag acagggccag tttatgcttc aaagtaatga gtacttttcc
     2821 ggcaaagtga aatcaatcgg cttttccagc agcagcactg gtcgcgccag cgtgggtgtt
     2881 atggttgaag gcgaatacac cttcagcacc gctgagccgg aagagatgac ggtaatcagt
     2941 ggcgcgctga atgtgttact gcctgacgcg accgactggc aggtgtatga agccggttcg
     3001 gtgtttaatg ttcccggtca cagtgagttt catctgcaag ttgccgaacc cacctcttat
     3061 ctgtgccgct atctgtaatt cctcgccttc cccttgaacg ggagggcatt tttctgaaat
     3121 atcctctctt taacccataa taatatttcc tt
//
LOCUS       DAJVYM010000247         3141 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVYM010000247 DAJVYM010000000
VERSION     DAJVYM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.252"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..957
                     /locus_tag="OQS60_003885"
     CDS             <1..957
                     /locus_tag="OQS60_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCS2389512.1"
                     /translation="YEPLLQSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHF
                     EYGTARAWGSFGYAIGAFFAGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVA
                     ADAGGVKKEDFIAVFKDRNFWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTR
                     LYGYLNSFQVVLEALCMAIIPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISL
                     VKLLHAIEVPLCVISVFKYSVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDH
                     AGYQTVFFAISGIVCLMLLFGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1486..1686)
                     /gene="torI"
                     /locus_tag="OQS60_003886"
     CDS             complement(1486..1686)
                     /gene="torI"
                     /locus_tag="OQS60_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCS2389513.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1809..2153)
                     /locus_tag="OQS60_003887"
     CDS             complement(1809..2153)
                     /locus_tag="OQS60_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389514.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2395..2997
                     /locus_tag="OQS60_003888"
     CDS             2395..2997
                     /locus_tag="OQS60_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389515.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          779 a          634 c          717 g         1011 t
ORIGIN      
        1 tatgaaccgt tactgcaaag caatttttct gtaggtctaa ttctgggagc gctctttttt
       61 ggcctggggt atctggcggg atgcggtttg cttgacagct tcaccgaaaa aatggcgcga
      121 aattttcatt tcgaatatgg aacagcgcgc gcctggggat cttttggcta tgctattggc
      181 gcgttctttg ccggcatatt ttttagtatc agtccccata tcaacttctg gttggtctcg
      241 ctatttggcg ctgtatttat gatgatcaac atgtgtttta aagataagga tcaccagtgc
      301 gtagcggcgg atgcgggagg ggtaaaaaaa gaggatttta tcgcagtttt caaggatcga
      361 aacttctggg ttttcgtcat atttattgtg gggacgtggt ctttctataa catttttgat
      421 caacaacttt ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt aggaacgcgc
      481 ctgtatggtt atctcaactc attccaggtg gtactcgaag cgctatgcat ggcgattatt
      541 cctttctttg tgaatcgggt agggccaaaa aatgcattac ttatcggtgt tgtgattatg
      601 gcgttgcgta tcctttcctg cgcgctgttc gttaacccct ggattatttc attagtgaag
      661 ctgttacatg ccattgaggt tccactttgt gtcatatccg tcttcaaata cagcgtggca
      721 aattttgata agcgcctgtc gtcgacgatc tttctgattg gttttcaaat tgccagttcg
      781 cttgggattg tgctgctttc aacgccgact gggatactct ttgaccacgc aggctaccag
      841 acagttttct tcgcaatttc gggtattgtc tgcctaatgt tgctatttgg cattttcttc
      901 ctgagtaaaa aacgcgagca aatagttatg gaaacgcctg taccttcagc aatatagacg
      961 taaacttttc cggttgttgt cgatatctcc ctatccctca accggaaaat aataatacta
     1021 aagtgcttag cccggctaat aatcacctaa tccaaacgcc tcattcatgt tctggtacag
     1081 tcgctcaaat gtacttcaga tgcgcggttc gctgatttcc aggacattgt cgtcattcag
     1141 cgacctgtcc cgtgtatcac ggtcctgcga attcatcaag gaatgcattg cggagtgaag
     1201 tatcgagtca cgccatattt cgctatcagg attctgtgtg atggttacat cgcccggccc
     1261 agggctgttt agtcatcagc gctttctgac agtgctgaga tttcaacctg ttgcagtaaa
     1321 aatgagtaga tataaggcaa gtgtgctgcc aaacccatct tttacggggt gaaggtagat
     1381 ttcgtttgaa gggtatctgg tgtcccctgc agacatctac ttgaggcggc aggggattga
     1441 ttggaatgct gttttttaga tttgaaaaat attttacccg ctattttacc cattggcgcg
     1501 gcttaagagc ttatttttga attcacaatg gtcacgatat aaccatcttg ctcgtccgtg
     1561 gataactttg gctttaggca ggtcgccgga cttaatccgg tcatagatga aggtcttacc
     1621 gaagccagta tcagccatga tgaatttcaa atcaaccagt gaatcaggtt gtagttcgtg
     1681 ttgcatgagt gctatctccg aatatggaat cgaacctgca aatcaggcaa taaaaagccg
     1741 cattgatgca gcaatggtag gtctggatat cttgagaaat gaacaggcct catcgagtgt
     1801 gaggcgggtt agtccttgcg tagctcgctg attcttctgt aagtctctgg tgctttgttc
     1861 ccgtacgtct tcatttcaga cttcaacaga gcaacgagtg aatcccattc gttgaggatt
     1921 cctttgaatg ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc
     1981 tgctcaacaa gtgcaacgca tcgccgaaag tctgctgcgt catggagagc gccgaagtga
     2041 tgaccataga tattcttttt caggccacat gcgattgagg caagagttgc gctactgatg
     2101 ccgacatcgc cagttgattg ccatttcaaa accttcatag ccaaatctga catttcttgt
     2161 ctccataaaa caaaactcgc cgtagcgagc tcagataaaa gaaatccccg tcagtgcgag
     2221 gatgctgttc attgctgcta tacacttttt tgctctcaac gtaagcggta gcccattctg
     2281 ttgggttggt cagttgcttt taggaaatgc tctttacccc ttaaacgtcg gctgaaagag
     2341 ctaaaatcca tgcaaaaaat ttacgcaatt ttgtgtatta ttgtgcagta agtaatgagc
     2401 tattttctgt gcaaaaaatg gatgataaat ttgtccgggg caggaaaaat tttatgggcg
     2461 ctaaacatga aaaaagattc gtatccttat ttgatttgca tgacagtttc agggctgatc
     2521 tttattttcc ttttcttctg gtggcgggca gatatctaca gggtcacgtt tcttaatcag
     2581 agtatatccc actattacat tctgtttagc atgggaatag cttttctgtt atctctgttt
     2641 tgggttaaga aggggatagt aaaacaaagc ggctggaaga gtctgtcagc ataccttaag
     2701 gtttatgcag ggatatgcat attttctgga ttttttctga ttatacccct tacaacacta
     2761 acttattttt tgcctggaga gacatcgtct tatgttgcac cgtatcggta tacttccggt
     2821 agttcaaaaa gttgttctgg agctgaggtg gatgaccccg atctacatga gaatattcgc
     2881 atttgctatc cgtatggcaa ttatgagtac gataatatta tctatgttga aaagaaaatt
     2941 aatacattag gtgcggtagt gacatatgca cagaccgcgc gtgatgatac tgaatgagat
     3001 agtatatagc gggcaagttt tagttaattt atcgaggtaa tataatttac ctcgactcgt
     3061 ttattctggt atgaatattt cgctttacga tcgattttta tctgatgata tcatgcggtt
     3121 ttcatatact gacttactgt c
//
LOCUS       DAJVYM010000248         3109 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVYM010000248 DAJVYM010000000
VERSION     DAJVYM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.253"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..1255)
                     /locus_tag="OQS60_003889"
     CDS             complement(<1..1255)
                     /locus_tag="OQS60_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS2389516.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="OQS60_003890"
     CDS             complement(1412..1621)
                     /locus_tag="OQS60_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389517.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="OQS60_003891"
     CDS             complement(1644..2354)
                     /locus_tag="OQS60_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2389518.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..>3109)
                     /locus_tag="OQS60_003892"
     CDS             complement(2870..>3109)
                     /locus_tag="OQS60_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389519.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          912 a          654 c          506 g         1037 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaaaaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccat atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccattcat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcctttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtc
//
LOCUS       DAJVYM010000249         3046 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVYM010000249 DAJVYM010000000
VERSION     DAJVYM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.254"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..173
                     /locus_tag="OQS60_003893"
     CDS             <1..173
                     /locus_tag="OQS60_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389520.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="OQS60_003894"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="OQS60_003894"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS2389521.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="OQS60_003895"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="OQS60_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="OQS60_003896"
     CDS             2191..>3046
                     /locus_tag="OQS60_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCS2389522.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          711 c          836 g          803 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAJVYM010000250         2994 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVYM010000250 DAJVYM010000000
VERSION     DAJVYM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.255"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            18..1076
                     /locus_tag="OQS60_003897"
     CDS             18..1076
                     /locus_tag="OQS60_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCS2389523.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OQS60_003898"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OQS60_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS2389524.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="OQS60_003899"
     CDS             2805..>2994
                     /locus_tag="OQS60_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCS2389525.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAJVYM010000251         2966 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVYM010000251 DAJVYM010000000
VERSION     DAJVYM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.256"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..216
                     /locus_tag="OQS60_003900"
     CDS             <1..216
                     /locus_tag="OQS60_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCS2389526.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="OQS60_003901"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="OQS60_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="OQS60_003902"
     CDS             complement(415..597)
                     /locus_tag="OQS60_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389527.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="OQS60_003903"
                     /pseudo
     CDS             696..1586
                     /locus_tag="OQS60_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="OQS60_003904"
     CDS             1589..2122
                     /locus_tag="OQS60_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2389528.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="OQS60_003905"
     CDS             complement(2151..2678)
                     /locus_tag="OQS60_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2389529.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="OQS60_003906"
     CDS             complement(2680..>2966)
                     /locus_tag="OQS60_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2389530.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAJVYM010000252         2942 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVYM010000252 DAJVYM010000000
VERSION     DAJVYM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.257"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..731
                     /gene="nagA"
                     /locus_tag="OQS60_003907"
     CDS             <1..731
                     /gene="nagA"
                     /locus_tag="OQS60_003907"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS2389531.1"
                     /translation="GTHNPNFVRKPDAALVDFLCENADVITKVTLAPEMVPAEVISKL
                     ANAGIVVSAGHSNATLKEAKAGFRAGITFATHLYNAMPYITGREPGLAGAILDEADIY
                     CGIIADGLHVDYANIRNAKRLKGDKLCLVTDATAPAGANIEQFIFAGKTIYYRNGLCV
                     DENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRMATLYPARAIGVEKRLGTLAAGKVA
                     NLTSFTPDFKITKTIVNGNEVVTQ"
     gene            740..1960
                     /gene="nagC"
                     /locus_tag="OQS60_003908"
                     /gene_synonym="nagR"
     CDS             740..1960
                     /gene="nagC"
                     /locus_tag="OQS60_003908"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS2389532.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            2008..2760
                     /gene="nagD"
                     /locus_tag="OQS60_003909"
     CDS             2008..2760
                     /gene="nagD"
                     /locus_tag="OQS60_003909"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS2389533.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT          697 a          767 c          778 g          700 t
ORIGIN      
        1 aaggcaccca taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt
       61 gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc ggaaatggtt cctgcggaag
      121 tcatcagcaa actggcaaat gccgggattg tggtttctgc cggtcactcc aacgcgacgt
      181 tgaaagaagc gaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg
      241 cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg
      301 acatttattg cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgtaacg
      361 ctaaacgcct taaaggcgac aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg
      421 ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg
      481 tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc
      541 tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg
      601 cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc cgcaggtaaa gtagccaacc
      661 tgacctcatt cacacctgat tttaaaatca ccaagaccat cgttaacggt aacgaggtcg
      721 taactcaata agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct
      781 cgtaaaacag cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc
      841 gcggattcag attgccgagc aaagccagct tgcccccgcc agcgtaacca aaattacgcg
      901 tcagcttatc gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg
      961 ccgcgctatc tccatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg
     1021 tcgtcacgac gccaccatca ccctgtttga tctcagcagc aaagtgctgg cagaagaaca
     1081 ttacccgctg ccggaacgta cccagcaaac gctggaacat gccctgttga atgccattgc
     1141 tcagtttatt gatagctacc agcgcaaact gcgcgagctg atcgcgattt cggtgatcct
     1201 gccagggctt gttgacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga
     1261 aaactggggg ctggtagaag ctctggaaga acgttttaaa gtgacctgtt tcgttggtca
     1321 cgatatccgt agtctggcgc tggcggagca ctacttcggt gcaagtcagg attgcgaaga
     1381 ctccattctg gtgcgtgtcc atcgcggaac cggggccggg attatctcta acgggcgcat
     1441 ttttattggc cgcaacggta acgtcggtga aattggccat attcaggtcg aaccgctggg
     1501 tgaacgctgc cactgcggca actttggctg cctggaaact atcgccgcca acgctgccat
     1561 tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgcgtgc cgctggacga
     1621 ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagtctgg cgtcggaagt
     1681 aattgagtat gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa
     1741 tccgcaaaaa attgttattg ccggtgaaat caccgaagcc gataaagtgc tgctccctgc
     1801 tattgaaagc tgcattaata cccaggcgct gaaggcgttt cgtactaatc tgccggtggt
     1861 acgttctgag ctggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat
     1921 gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct
     1981 tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac
     2041 ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt
     2101 atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac cgggcaagat
     2161 ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt ttatacctct
     2221 gcgatggcga ccgccgattt cctgcgtcgc caggaaggca agaaagcata tgtggtgggc
     2281 gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct
     2341 gatttcgtga ttgttggcga aacgcgttcc tacaactggg acatgatgca taaagcggcc
     2401 tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacaccca cgggcgcggt
     2461 ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg
     2521 ttctatgttg gtaagcccag cccgtggatc atccgcgcag cattaaacaa aatgcaggcg
     2581 cattcggaag aaacggtgat tgtcggcgat aacttgcgca ccgacattct ggcgggcttc
     2641 caggcaggtc tggaaacgat tctggtgctt tctggtgttt catcgctcga cgatatcgac
     2701 agtatgccgt tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga cgttatctga
     2761 ataccaccca cgtctgcgga cgtggttggc tctgatgaca aacgccaaac tgcctgatgc
     2821 gctacgctta tcaggcctac gcagctcctg caatatattg aattttcatg cttttgtagg
     2881 ccggataagg cgttcacgcc gcatccggca tgaacaaatc gcactttatc agcaatttgg
     2941 cc
//
LOCUS       DAJVYM010000253         2893 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVYM010000253 DAJVYM010000000
VERSION     DAJVYM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.258"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..493
                     /locus_tag="OQS60_003910"
     CDS             <1..493
                     /locus_tag="OQS60_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS2389534.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="OQS60_003911"
     CDS             493..1083
                     /locus_tag="OQS60_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS2389535.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="OQS60_003912"
     CDS             1070..2110
                     /locus_tag="OQS60_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2389536.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="OQS60_003913"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="OQS60_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2893
                     /locus_tag="OQS60_003914"
     CDS             2682..>2893
                     /locus_tag="OQS60_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS2389537.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          752 a          633 c          824 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattaccccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAJVYM010000254         2840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVYM010000254 DAJVYM010000000
VERSION     DAJVYM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.259"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1120..1455)
                     /locus_tag="OQS60_003915"
     CDS             complement(1120..1455)
                     /locus_tag="OQS60_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCS2389538.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1584..1931
                     /locus_tag="OQS60_003916"
     CDS             1584..1931
                     /locus_tag="OQS60_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389539.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1951..2541)
                     /locus_tag="OQS60_003917"
     CDS             complement(1951..2541)
                     /locus_tag="OQS60_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389540.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(2538..2798)
                     /locus_tag="OQS60_003918"
     CDS             complement(2538..2798)
                     /locus_tag="OQS60_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389541.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          796 a          671 c          589 g          784 t
ORIGIN      
        1 agtcacataa accaccgatt gagtgacagt tactgaaaca actatttcga cacaaaatct
       61 cgtctattca acatcatgca aatttgtcag tgactgaatt tagtgacaga accctctgga
      121 attctgccgt gtaaccactt atatgcagac ctgccccgtt gctctgggtt ttcaggctgc
      181 gatgagtgat gagcgatgat ttccatacag atgatatatc gactggaatt gattgcctgc
      241 aattagattt agttttattg tgtggatcgt tttagttagt ggggatgaat ttccgataaa
      301 ttaaccaatg gaaaggagat gatatgaata aaaagataac tggattatta ataattagtc
      361 tggcgtcaat tttcttaatt gattgcaccc agtgccatta acatctatct acacaaaaat
      421 agaggaaaac aattttgata tatcaaataa ttacaagcag ctccattctt tttattgttt
      481 gggggattac atctataata attagaacaa agaattattt ttctaagaag aaaacagacc
      541 ttaatataag cttttttata gccttcccta tttctataac aatcctttta ttttgccgag
      601 gtatattata aatataacat aatacatcta tatggggtgt acatatcccc acataaaaac
      661 acatactctc attaacacgg gaagaaacta tgttttccaa ttctcactac atgtacataa
      721 tcgttcttgt actatggttt agtattctca agtacttaca gagaaagaat agcatattac
      781 atcctttatt ttcactaatc ctgacaccac taaccatttt aatcattaga cttatgatag
      841 atttaacata caaccaataa attataaaca aaataaaaat agggaacatt atatgcttta
      901 taaaattttc acattaaaca caaagataaa aacaactatc attacaatat gtgcattatc
      961 atataatctt acagcgcaag cttccccgtc atacagacct cgtgatcgag ttaataagac
     1021 tatgatagaa ctacaaacta acggagtaga acggcaactt ttagataatt cttgtggcat
     1081 aagttctggg gtatttaaat aaaatataag acaggctgtc tatcttacag acagcctttt
     1141 tataataaca gaaagaatat atatcatact taacggaaga gataatatga aaacagtacc
     1201 cgtataaaca gcaagcaatc ccatttttcc aggtgtatca gcaaaaaaaa cgctgttcca
     1261 gaaatcaggc cgggtaaatt tcagagctgt atcttcaata taccattttg caaccggata
     1321 caaaaccatt ccgcagagag aaatacacca gaacagtaat ctgtatttaa aatcataatc
     1381 ccatgacata tacagcatat atcctcccgt cacccatccc caccacatat tattaaaata
     1441 atattttctg ttcatctgcc agttttcctt tcttttgcac ttttgtcagt gtactgatgc
     1501 atgacaacat aaccacagca tgataataat aatcaataac aataagctgt gtcacgttta
     1561 catcatggaa cggaggtgct cctgtgactt ttgagcagaa aaaagcccgg gccattgccc
     1621 tgatggacag caaaaagatg tggcggagca attatgcccc tcctcttctg cgtattctct
     1681 ggcggctggg tatccggctt ccgccattac cctttatgcc gttctggcag gtcacggtgc
     1741 tgacgggggg cttatggggc atttcctggg gatgtgcaat gtggtttatc tactgggggc
     1801 cgtcaggaat ggtggcaggt gaggccatta ttataagtat cacggggggt tttttgtccg
     1861 gactgctcat ggcctctttc cactggtggc gcaggaaagt taaccgactg ccaccatggg
     1921 atgatgtgta aaccgtgcac aggcgtgttc ttacggagcg gcactttcat ttatctgtac
     1981 gcgaacctcc attcctgcat cgtgacaggc cggcagccac tgcgccactt ccagcggatc
     2041 gccctcccgg cgtaccaccc agccttcttt attccataac tgcagacagg tgctgccgtc
     2101 gagacgcacc acaaaatccc cacggcaggc ctgatacgtt atgccgttca gctccaccgc
     2161 cagatcagtg gcagaagtgc cgcctgcggg cagacctgct gccgggacca gcggcagtac
     2221 gacgacttct gccgcacgct gttctgcccg gatgcggtcc tgttctgcca gcagcagagg
     2281 ctgtgccacg gcccggccgg cgtccgtcag ctccactgcc agctgcaggt tcggagcgcg
     2341 cagggtacgc agccagccgg catcctccag acgacggcag gaggcgcgca ggtttggccc
     2401 gtagaccggg gcttccccgc tttgctccag cacccgttct atgtcccgcg tcagcacggg
     2461 tcctggccgt ttttcatcaa gggcagccag cacaatcagc actctccgct gcagtggcga
     2521 cggtcggcgg ctctgagtca tttccagtca tcctccggcc agatgagctt tctgaggcgc
     2581 tgatggtcgg cgtcaatgat ctcctgtctc tttatggcat tcattgaggt atagccattt
     2641 atggccagtt catgccagaa gatcagcacg gctccggctt tggctgactc gcagagataa
     2701 gttacgccgt ggctgtccag attgcattcc cggatgcgtt tttccgcaaa ccgggtcagt
     2761 tgttcatagc tgagtgaaaa cgtcatttcc tgatccatcc tgcacgttcc atctgataat
     2821 gttctgacag gtaaaaatca
//
LOCUS       DAJVYM010000255         2816 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVYM010000255 DAJVYM010000000
VERSION     DAJVYM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.260"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..991
                     /locus_tag="OQS60_003919"
                     /pseudo
     CDS             1..991
                     /locus_tag="OQS60_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1007..1756
                     /locus_tag="OQS60_003920"
     CDS             1007..1756
                     /locus_tag="OQS60_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2389542.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1758..>2816
                     /locus_tag="OQS60_003921"
     CDS             1758..>2816
                     /locus_tag="OQS60_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389543.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          886 a          566 c          585 g          779 t
ORIGIN      
        1 taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc agcagtaatg
       61 tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa aacgtctccg
      121 cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa ctgtacctaa
      181 atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa cgaaatcagg
      241 ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat aattcctgga
      301 gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca
      361 gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca cagcgagaca
      421 gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt cacggagccg
      481 ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca gatggtgcag
      541 aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc ggagttattc
      601 catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat cttccagaaa
      661 atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct attggctacg
      721 ccaaacttga taccccaagg gctatcaaat catgggggtt attcgcctgg cagatggtag
      781 tcacccacca ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt
      841 tgctgatggc ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg
      901 ctggggtgat aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac
      961 ggttatttta ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa
     1021 cacaaagaat ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg
     1081 tgccatcatc ccggaccgta cccgtatcat aatgaatgag tcagataaag gggaagcatt
     1141 aaaacttact aatcaaagca aaaatctacc ctatctggcg caaacatgga tagaagatac
     1201 gaaaggcaat aaatcacgtg actttattgt cacggttccc cctatggtac gtttaaatcc
     1261 cagtgagcaa attcaaataa gaatgataag tcaggaaaaa attgctcaac tgcctaatga
     1321 cagagaaacg ctcttctatt ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa
     1381 tgtgatgcaa gtcactatgc agcacgcatt gaaactattc tggcgaccaa aagctattga
     1441 attagaagat gatggggtaa tgtcttacga aaaagttgaa atcatccgta gaaatgatgg
     1501 ttcgatccgc tttaacaata agatgcctta tcacgttacg ctgggttata tcggaacaaa
     1561 cggaatcaca atgttgccac aaacgcaaag tttaatggta accccttata gccatgcaga
     1621 tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt tatatcaatg attttggtgg
     1681 attaagtttt tatgaaataa actgcccgac ggttaataac tcttgtaatg tttccgtagc
     1741 caaacgagat aaataatatg cgctacttat taattgtaat tatatttatt ataggtttta
     1801 gtgcattacc tgtatgggca atggactgct atgctgaaca tgaaggggga aataccgtag
     1861 tcattggtta cgtaccaaga atttctatcc ccagtaatgg taaaaaaggt gataagatct
     1921 ggcaaagcag tgagtatttt atgaatgtct tctgtaataa tgcattacct gccccatcac
     1981 ctggagaaga atacccatca gcctggacta atataatgat gtttttacca ggcggtcagg
     2041 acttttataa tcagaactcc tatatttttg gcgtaacata caatggagtt gattatgatt
     2101 cgactgcccc taatgctatc gcagcaccag aatgcattga tataaaagga gcgggtacct
     2161 ttaataatca ctataaaaac cccgctgtct gtagtggagg tcctgagcct caattatcag
     2221 taacctttcc agctcgcgta caactctata ttaaattagc aaaaaatgcc aatagagtta
     2281 ataaaaacct ggtgttacct gatgaatata tcgctctaga gtttaaaggt atgagtggcg
     2341 caggagctat agaagtagac aaaaatttga ccttcaggat tcgtggatta aataatattc
     2401 atgtccttga ctgctttgtt aatgtcgcac tggaaccagc tgatggcgtt gttgactttg
     2461 gtaaaataaa ttcccgaaca attaaaaata ccagcgttag tgagacgttt agtgtagtca
     2521 tgacaaaaga tcctggtgcg gcctgtactg agcaattcaa tattttaggg agctttttca
     2581 ctacggatat tttgagtgat tatagccatc tggatatggg taatggactg ctattgaaga
     2641 tatttcataa agatggaaca gcaacggaat ttaaccgctt ctcacaattt gcttcttttt
     2701 catcgtctag tgcaccttcg gtcaccgcac cattcagggc agaacttagt gcgaacccgg
     2761 cagagactgt tgttgaggga ccgtttagta aagacgtaat cctcaaaatc acctat
//
LOCUS       DAJVYM010000256         2806 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVYM010000256 DAJVYM010000000
VERSION     DAJVYM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.261"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            148..1047
                     /gene="pgrR"
                     /locus_tag="OQS60_003922"
     CDS             148..1047
                     /gene="pgrR"
                     /locus_tag="OQS60_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS2389544.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1384..>2806
                     /gene="mppA"
                     /locus_tag="OQS60_003923"
     CDS             1384..>2806
                     /gene="mppA"
                     /locus_tag="OQS60_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS2389545.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQAS"
BASE COUNT          756 a          605 c          674 g          771 t
ORIGIN      
        1 tattcatcat tccgttcctc atggagttgt cggttcgttt taacggttgg tgatatcact
       61 atagatattg atcattaagt tgattagacc caaaattatg attagaccta tcatttaaat
      121 gattaataga ttgcatggag atactgaatg aagcgtgaag aaatcgctga tctgatggcg
      181 tttgtcgtcg ttgcagagga gcgtagcttc actcgtgcag cagcccgcct gagcatggcg
      241 cagtcagctt taagccagat agtgcgtcgt atagaagaac gattgggatt gcggcttctg
      301 acgcgaacca cgcgcagcgt tgttccaact gaagcgggcg agcatctttt gtctgttctt
      361 ggccctatgt tgcatgacat agattcagcc ctggcatccc tgagcgagct gcagaaccgc
      421 ccatccggga caatacgtat tactactgta gaacatgcag ccaaaacgat attgttacca
      481 gcaatgcgca cattcctgaa atcgcatcct gaaattgata ttcagctcac cattgattat
      541 ggtttgaccg atgtcgtttc tgaacgtttt gatgcaggcg tccgtctggg tggggagatg
      601 gataaagata tgatcgccat tcgaatcggg ccagatatac caatggctat tgttggctca
      661 ccggattatt tttctcgccg aagtgttcca acgtcagtgt cacaattaat agatcatcag
      721 gcaattaatt tgtatcttcc cacatcgggt acagcaaatc gctggagatt aatacgcggt
      781 ggacgtgaag ttcgtgttcg catggaaggt cagcttttac tgaatacgat agacctgatc
      841 attgatgctg caattgatgg gcatggattg gcgtatctac cttatgatca ggttgagcgg
      901 gctattaaag aaaaaaaact gatacgtgtt ttggataaat tcacaccaga tttacccggt
      961 tatcacctgt actatccaca ccgtcgacat gctggctcgg cattctcatt atttatagat
     1021 aggctgaagt ataaaggtgc tgtttagcac tacttgctga tacattaatt taatttttct
     1081 cttaacgtat tctcagttcc tttcaacgtt ttggttattt tttattcttc gtacaatggc
     1141 gacagatgct gattatgata ccgaaaactg gtttgaacgt gcgaagcctg aacgtagtgt
     1201 tcggtgttct atgtgcttta ccgcattttg tcgtctatta tttacactaa atctgattta
     1261 atatattgat acataaaaca ttttatgctc cctgtgtcac ttttttgatg gaagttgttt
     1321 gcatttcttt aaggcgaaac aaataattac gcatcaattt taatgtcggt tagagggaaa
     1381 cttatgaagc actctgtttc agtcacgtgt tgtgcgctgt tggtcagcag catttctctt
     1441 tcgtatgctg cagaagttcc gagcggcaca gtactggcag agaagcagga gctggtgcgc
     1501 cacattaaag atgagcctgc gtcgctggat cccgctaaag ccgtgggcct gccagagatt
     1561 caggtcattc gcgatctgtt tgaaggactg gtgaatcaga acgagaaagg ggagattgtc
     1621 cccggcgttg cgactcagtg gaaaagtaat gacaaccgta tctggacttt taccctgcgc
     1681 gataacgcaa aatgggcgga tggcacaccg gtaacggcgc aagattttgt ctacagctgg
     1741 cagcgtctgg tggacccgaa aacgctatcg ccatttgcat ggtttgccgc gctggcggga
     1801 atcaacaacg cacaggcgat tattgatggt aaagctacgc cagaccagct tggcgtcacc
     1861 gcagttgatg cccatacttt gaaaattcag cttgataaac cgttgccgtg gtttgtgaat
     1921 ttaaccgcta actttgcctt cttcccggtg caaaaagcca acgtagaaag cggtaaagag
     1981 tggacgaaac ccggaaatct gatcggcaat ggcgcttatg ttcttaaaga tcgcgtagtc
     2041 aatgaaaaac tggtcgtggt accgaatacc cattattggg ataacgccaa aacggtactg
     2101 caaaaagtga ccttcctgcc aattaatcag gaatccgcag ccactaagcg ttacctcgcg
     2161 ggggatattg atatcaccga atccttcccg aaaaatatgt atcagaagct gttgaaggat
     2221 attccggggc aggtttatac gccgccgcag ctcgggacct attattatgc gtttaacacg
     2281 caaaaagggc cgacggcaga tcagcgcgtt cgtctggcat taagtatgac gatagatcgc
     2341 cgcctgatga ccgaaaaagt attagggacg ggcgaaaagc cagcgtggca ttttacacca
     2401 gatgttaccg ccggatttac gccggaacct tcgccgtttg aacaaatgag tcaggaagaa
     2461 ctgaatgcgc aggcaaaaac tttgttgagc gcagctggtt atggtccgca aaaaccgctg
     2521 aagctgacgc ttttgtataa cacttcagaa aaccatcaaa aaattgcgat tgctgtagca
     2581 tcgatgtgga aaaagaacct tggcgtagat gttaaattgc aaaatcagga atggaaaacc
     2641 tatatcgata gccgtaacac cggcaatttt gatgttatcc gcgcctcatg ggtgggggat
     2701 tataatgaac cctccacttt cctgacatta ttaacgtcaa cgcattcagg aaatatttca
     2761 cgctttaaca atccggcata cgacaaagtt ctggcccagg catcga
//
LOCUS       DAJVYM010000257         2784 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVYM010000257 DAJVYM010000000
VERSION     DAJVYM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.262"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..552)
                     /gene="fliA"
                     /locus_tag="OQS60_003924"
     CDS             complement(<1..552)
                     /gene="fliA"
                     /locus_tag="OQS60_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS2389546.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVME"
     gene            complement(717..2291)
                     /locus_tag="OQS60_003925"
     CDS             complement(717..2291)
                     /locus_tag="OQS60_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS2389547.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            2546..2784
                     /locus_tag="OQS60_003926"
                     /pseudo
     CDS             2546..2784
                     /locus_tag="OQS60_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          716 a          659 c          595 g          814 t
ORIGIN      
        1 ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcgggt tttctcgctg
       61 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcgcgccact catcgtagga
      121 gaagagctgg ctgttattgg tgtcgagcaa catttggcga taatcggcaa tatcgatccc
      181 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
      241 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
      301 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
      361 tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc ccgcctgtag
      421 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
      481 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
      541 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
      601 tgcaggtttt ttaatcggac gattagtggg tgaaatgagg ggttatttag gggttaccgg
      661 taaattgcgg acagaaaaaa ccccgccggt ggcggggttt gagcgataag tgtaaattaa
      721 cgcagcagag acagtacgtt ctgcgtggtc tggttagcct gtgccagaac agaggtaccc
      781 gcttgttgca ggatctgcgc acgagacatg ttagacactt cggttgcgta gtcagcatct
      841 tctatacggc tacgggcaga agacaggtcg tttacggtgt tgccaaggtt ggtgatggca
      901 gagtcgaaac ggttttgtac tgcacccaaa gaactgcgca acttatcaac ttttgccaaa
      961 gcttcgtcta atttggataa tggatttgtg gttgctaagg ctttcaaatc tgaggcactc
     1021 agttcagcac ctttggttgc cgtcttattc gtgatattgg ctgtataaac cttatcatct
     1081 tcacgcataa ttgcctgtgc agaaccatca ttttttactg cccattcaac tgccttacca
     1141 ttaggatcag taataacaga cgtgttacct gtagcggcat ctacatttac tgcaatgtag
     1201 gtgccgtctg atgttttagc agcaaatgaa ttaggtacag gattcccttg ggtatcttgt
     1261 acttggtgga catctaggtt ttttccccca aaataaacag acattggtgc atcattataa
     1321 gctaatgctt tacccatttt acccgcagct aaatcaactg atgtggcaga accattatct
     1381 tcaacaaatg cagttacacg accaccaaag tttgctaact gaacatcctt accacccacc
     1441 tgaacttttt cataacctgt agagttttgt ttcaataatg taaaaccgtt cgtgttggta
     1501 ttattatctt gaccaacaaa ataagtctgc ccttccacct gtacttgata aacttttgtt
     1561 cctgcagtac cggtcgcatc agttgtatcc tttgtatctt gtacattacc ggtaccaaaa
     1621 taagtttcaa catcagcatt tttataattg ctaccgaatg ctttagcacc tcccacacta
     1681 ccatcagttg catttacctt agttaagtca ggagcggctt cgctcttaag tgcaaccgca
     1741 gaggacattg gaacttttcc tagtgctacg ctaaagttat caagccccaa ggttttagca
     1801 tcaattttat caagggcaat ctcaatggtt tcaccatcgt tcgcacctac ttgaattttc
     1861 atagtctgat cagaagccag aacttttaca ccattaaatt gtgtctggtt agaaacacga
     1921 tcaatttcac taaggcgttg tgtaatttca tcctgaattg aattcaggtc ggagtcagag
     1981 tttgtaccat tagtggcctg tactgacaat tcacgaatac gctgtaagtt attgttgatt
     2041 tcagaaagcg caccttcagt ggtctgcgca acagaaatac catcattcgc gttacgggaa
     2101 gcctgagtca gacctttaat atttgctgta aaacggttag caatcgcctg acctgctgcg
     2161 tcatctttag cactgttaat acgcaggcca gaagagagac gttcaatggc ggagctcaga
     2221 gaagactgag atttgttcag gttattctgg gtcaacagcg acaggctgtt tgtattaatg
     2281 acttgtgcca tgatcttttc cttatcaatt acaacttgat gttattgggc tgttgcccac
     2341 ggtttctcac cgtacccctt gtatcggcac ctgaatttcg aactttagaa aattttagtt
     2401 tctctcctgt tttttcttaa cggctgaaat atccgtaata tacaactcta ctctgcgcat
     2461 catcttcgtg aatcaactat taaactttgc gcacaaattg ccgataaccc gcttatctac
     2521 tgttttcaat caaaaggaat taggtatggc ttcatttaca tctcttggcg ttggttcgaa
     2581 tctgccgctg gatacattgc taaacaacct gaccatcgct gaaaaaaaac gcttaaaccc
     2641 aattactcag caacagagtg acaataccgc acgtctgaca gcctatggca cattaaaaat
     2701 gtgcattaga aaaattccag actgcaaata ctgcgttaaa taaagccgat ctgttcaaaa
     2761 gcactaccgt taccagtagc aatg
//
LOCUS       DAJVYM010000258         2762 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVYM010000258 DAJVYM010000000
VERSION     DAJVYM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.263"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(24..296)
                     /locus_tag="OQS60_003927"
     CDS             complement(24..296)
                     /locus_tag="OQS60_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS2389548.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(299..577)
                     /locus_tag="OQS60_003928"
     CDS             complement(299..577)
                     /locus_tag="OQS60_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389549.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(578..1135)
                     /locus_tag="OQS60_003929"
     CDS             complement(578..1135)
                     /locus_tag="OQS60_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389550.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          778 a          544 c          518 g          922 t
ORIGIN      
        1 atattcgaat ttaatacata acattaaatc tccaggagta aattgtttgg gtggcaattt
       61 cttttattac catccctgaa gataatcttt ttagttgcct gaacttcatg atgcaaaaac
      121 cagatgacat acttcagata ataatcacga gtgccataac gaatcattgg attaccgtaa
      181 gcatcatctc ttgtcttaac ctctccacca tcaatccatt caagcattcc tgtctcataa
      241 ttaatcatga agtaagttgc aaagaaccgt ttaatatcat ctcgccattg cttcataatc
      301 agacctcctg agacagtttg tgaattttga tacttagtaa ccttgctaaa tccagtccaa
      361 aagcatttaa ggatttcaga ctatcaaggt catcttgctt aatcctgata tggtgtcttg
      421 cagcctctaa gcgactatta agcaaatcct gttcgttaat atcacggtta attgcaagct
      481 tcatcactgc tgagtgtggc agacctgtaa tgtttttgat tcggttcagt tcatcaaagg
      541 ctgtctttgt aactctggtt aatgtgtgtt tattcattta aaaatcctct gtaactaaat
      601 aggggtcaaa ctcgaaatct ggttgctcgt tttccattgg ctcaatattc ccaacggcaa
      661 tatgatgttc aacaatgcca attaatttcg cgcacatcac agcaagcaaa gcgcaatatt
      721 tatcgcttgg taattcgtaa ctaagtctgg ttgcctgtcg ctgaagatga tttgtcacgg
      781 tggatgcgtt aatttcgtca agattcttta gcagataaaa tagttttttg ttgtatttcg
      841 ggtggccttc acttctacag aacccaataa aaacatcatc aagcttgcct ggtaactgct
      901 cgtaactgtc aaagttagcc agataatttt taatagtatt ctttgttgtt ttgcgtactg
      961 gcataaatct gtctcgtggc gtaatgctac gaatgaccgc ttcaatgttc ttacgttcgg
     1021 atttgctaaa ggttgggact tcgtagctgg tggggttgtt tgtgtaagcg taggagttgt
     1081 gcttgttgcg ttctcttgat gcctgaagca tcgctctaaa ttctgaatac tgcattcatg
     1141 ttctgactct cataagatgt tattattctg ttaacctata tacaatatta gcattttcaa
     1201 agactaatgt caagtatttt taacattttc gtaacatctg tggtataatg ggtttatggg
     1261 gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt gcacacagac
     1321 ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat aaggtcaggt
     1381 actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg gtaaatctcc
     1441 ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg attgcaggcc
     1501 tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc ggtcgtacat
     1561 ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct tcaaaacccc
     1621 tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt ttgtgctatc
     1681 tattaaaaaa atagcttgac agaaccggaa agccgtatct atcaagcatt cacctctctg
     1741 acagtaattt ctaaaagttc ttatttttca ataagttgta attagccata tatagagaac
     1801 taaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatacattt tattgattta
     1861 ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt acttaacaga
     1921 cttttaagag actttgaaac agcctgttta acaggtcact acctctggtc ttatcgttcc
     1981 cttctttcta ggtggttatc tgttaagagt ttttcagttt ttattcttaa gataattgtt
     2041 aagatgatac tctcccttcg ggttatatcg tatctcttct cccgttaaat atcccttcac
     2101 agtcagacta aataacacct gtgtagttct tggtagaatc tttcaaggta acttagctct
     2161 gagtttatga agttgaccta tgagatagac tctaccgaat ctgttagtca attgttgctt
     2221 aggtgctgct aaggcttact tagtggaaag agaggggata tgtgaccata ggggaacccc
     2281 tcctgttaaa agactactta gaagtctgag gttttagtaa aatctgtgaa tatggcttct
     2341 cagacgctct gtaacgcatt ctaagcgatt ttaccccttt attttaaggt ggttagtaca
     2401 tccctatagg ttacccctaa aaatggggtt aaaactcgct acagagcttt ctatgagatt
     2461 agcttctaat cgaatcaact atgaggtttg gtaggattgt gcaaaatctg tacagtaact
     2521 tttcagaatc tgaaaatttt tataaaaaat gactagccct ctttgaatct catcttcccc
     2581 gaatcttcaa aaatatctga cagatgtctg tagctgctaa gtacccgtca caccagctat
     2641 gtgaccccct tggcaaaaaa gccaaatatc agctacatcc atgtaactat tcagtaactt
     2701 atttagcaag tctgtaataa gttgctctag agattcctaa ctcttcctga atctgcaacc
     2761 tc
//
LOCUS       DAJVYM010000259         2722 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVYM010000259 DAJVYM010000000
VERSION     DAJVYM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.264"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            407..1123
                     /gene="nanC"
                     /locus_tag="OQS60_003930"
     CDS             407..1123
                     /gene="nanC"
                     /locus_tag="OQS60_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS2389551.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1143..2249
                     /gene="nanM"
                     /locus_tag="OQS60_003931"
     CDS             1143..2249
                     /gene="nanM"
                     /locus_tag="OQS60_003931"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS2389552.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2314..>2722
                     /locus_tag="OQS60_003932"
     CDS             2314..>2722
                     /locus_tag="OQS60_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS2389553.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSER"
BASE COUNT          830 a          554 c          618 g          720 t
ORIGIN      
        1 gtcaatgaga cagggcatct cgcaatctat ggcaaacatc acttcagttc tttctcatcg
       61 ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa tgagatcaac agacactcta
      121 ttttatgact ctgggtaaaa tggattgagt aagtgatata gcttacgaac attcaaatca
      181 attaaacatc agaagagatt ttatactcag gtatttaatc tgattctctg tttatttaaa
      241 taatgtggaa agagaatttt cacaggagac cttatacaaa aatataaaat aagctacccg
      301 ttgccaaaga cactataagc ctggcaaaaa atattacaca acataaatgc taattgttta
      361 tgcgggcttt gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa
      421 aatactttct ggcgtattat tactgtgctt ttcgtcacca ttaatttctc aggctgcgac
      481 actggacgta cgtggtggat atcgtagcgg aagccatgcc tatgagactc gactcaaagt
      541 cagtgaggga tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat
      601 tcatgataat aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc
      661 gattaaactt gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa
      721 cggcacacgc tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt
      781 tggcattcgc tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat
      841 gtctcgtgat aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt
      901 caccttcgca tggcaaacga cgctatacag caaacagaat gattatcgct atgcaaacca
      961 caagaaatgg gcgacggaaa atgcctttgt tctacaatac catatgacgc ccgatattac
     1021 gccatacata gaatatgact accttgaccg ccagggtgtt tacaacggca gagataattt
     1081 atcggaaaac agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca
     1141 caatgaataa aactataatg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt
     1201 ctgtattacc ggaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca
     1261 ctgtctacat tggtttaggt agcgcaggta cagcatggta caagctagat acacaggcca
     1321 aagataaaaa atggactgcg ttagctgcat tccctggtgg accgagagat caagcaacct
     1381 ctgcatttat tgatggcaat ctatatgtgt ttggcggcat tggcaaaaac agcgagggat
     1441 tgactcaggt atttaatgac gtacacaaat acaaccccaa aaccaatagc tgggttaaat
     1501 tgatgtcgct cgcgccgatg ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg
     1561 cttatgttac tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg
     1621 aggctggaaa agattcaacc gctatagata aaatcaacgc ccactatttt gacaaaaaag
     1681 cagaagatta tttcttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga
     1741 gttacgctgg cgaatcgccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg
     1801 ataaaacctg gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg
     1861 aacttgattt caccggtaat aatttaaaat ggaataagct tgatcccgtc tcatcaccag
     1921 atggcgtcgc tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg
     1981 ccggattcaa aggttcacga gaaaattacc agaacggtaa gaactatgcg catgaaggcc
     2041 tgaaaaaatc atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg
     2101 gtgaattatc gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtttattaa
     2161 ttattggcgg tgaaactgca ggcggcaaag cggtgacgga ttcagtttta atttctgtga
     2221 aggataataa agtcacagta caaaactaac gcttcagggc cccggtaaag gggccctgct
     2281 atcaatttgt catttgtatt acgcgaaaat tatatgaacg caataatatc gcccgattat
     2341 tactatgttc ttaccgttgc tggtcagtct aatgccatgg cgtatggcga aggactgcca
     2401 ttaccggaca gggaagatgc gcctcattcc agaattaaac aattagcgag atttgcgcat
     2461 acgcatcccg gaggcccgcc atgtcacttt aacgacatta ttccactgac tcactgccca
     2521 cacgatgttc aggatatgca gggttatcac catcctctgg caacgaatca tcaaacacag
     2581 tacggcaccg ttggccaggc actgcatatt gcacggaaat tactgccctt cattcctaat
     2641 aatgcaggga ttcttatcgt tccgtgttgc cgtggcggat cggcttttac cgcgggcagc
     2701 gaagggacat attcagaacg gc
//
LOCUS       DAJVYM010000260         2720 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVYM010000260 DAJVYM010000000
VERSION     DAJVYM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.265"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            46..621
                     /locus_tag="OQS60_003933"
     CDS             46..621
                     /locus_tag="OQS60_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389554.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
     gene            952..1998
                     /gene="mdtG"
                     /locus_tag="OQS60_003934"
                     /pseudo
     CDS             952..1998
                     /gene="mdtG"
                     /locus_tag="OQS60_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            2081..2455
                     /locus_tag="OQS60_003935"
     CDS             2081..2455
                     /locus_tag="OQS60_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCS2389555.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(2456..2683)
                     /locus_tag="OQS60_003936"
     CDS             complement(2456..2683)
                     /locus_tag="OQS60_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS2389556.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
BASE COUNT          726 a          636 c          662 g          696 t
ORIGIN      
        1 agacaagaca aactaaccgt ttaatgacta cccagaggga tatctatgtc acgaaccttt
       61 gcttactgcc gtgtatcagt agagacttcc accacagaga accagattca agccattaag
      121 aaggctggct atgatattcc taagtcacga gttatagagg aggttgtctc tggttcagtg
      181 tcagctatgg atagggacgg atttgcgaat ctggtcaacc ataagctaga agaaggtgac
      241 acccttgttg ttcttaaact tgaccgtctg ggaagggata acatcgacct acagaataca
      301 gtgacccttc ttaatgataa gggtgtgaag ctcatctgtt tagatttacc cgttaaagac
      361 ctttcaagct cagagggtaa gttggttcta cagctattta gtacctttgc agagtttgaa
      421 agaaaccgta tcagagagcg cactaaacag ggtctagagc gtgctagagt tcaaggtaag
      481 gtacttggaa gacctatagc gagtgacaca actagagcgg tcttagagta caaggaaagg
      541 ggactatctc agtctcaggt agctaaggtt cttaacctct ctgtaaggac tgttagcaga
      601 cactggacca agaccctgta agacccttaa tagactacac agcctccttt atagggggct
      661 tttttgtacc tgtgacatag ctatcaaggc gctatgtaga ggtctttata gcgttgtata
      721 ggccagtata cagcactttt taggaatgga gagggaaacg actagacata acgctaagtt
      781 atctattcag actgcacaag aatctttatt gatttcaata agttacctta atagtatcta
      841 ttcatctcta taccaaatct acacagtctt ctaccaataa tactgtataa acatacagca
      901 ataacctgta taggctcact tatcaggtag tgcattaaga tagcatatat gagcattaca
      961 tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccga
     1021 aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt gatggggctg
     1081 gcacaaaata tctggcagtt tttgatcctg cgggcgcttc ttgggttact tggcggattt
     1141 gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag cggctgggcg
     1201 ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc tcggcccaat ggctggcggc
     1261 ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata
     1321 ctctgctttt tcgtcaccct gttttgcata agagaaaaat tccagccgat cagcaaaaaa
     1381 gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact ggtactcagc
     1441 ctgtttgtca ctacgttaat catccaggtg gcgacgggct caattgcccc cattctgacg
     1501 ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc
     1561 gcctcggtgc caggcgtggc ggctctgcta agtgcaccac gactcggcaa acttggcgat
     1621 cgaatcggac ccgaaaagat cctgattacg gcgctgatct tttctgtact gctgttgatc
     1681 ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt gctcggtgcc
     1741 gccgatggtg cactactccc cgccgtacag acactgttag tttataactc gagcaaccaa
     1801 atcgccggac gtatcttcag ctataaccaa tcgtttcgtg atattggcaa cgttaccgga
     1861 ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt tctcgtcacc
     1921 gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata
     1981 ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga atcagctatc
     2041 cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct
     2101 tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc
     2161 cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat
     2221 catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacctatgct
     2281 tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag agatccgcca
     2341 ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc agcttgagcc
     2401 accgctggat accgaggaag gacacgctgc agctgatgag tgggatgaac gttaatcact
     2461 catacgggcc atgatgagtg tcgatcggca gcagtaaagt atcaaaaacc agggagaatg
     2521 gcagatcaag gattgtgaca taacgccaag cggagtcacg cacatcccat tgcacaccgg
     2581 gataatattg gttgccgtgc ccctgccccg gtatagtgcg gctaatgatg ctgccacagc
     2641 cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac ctcttccgta
     2701 aaaaaatacc ggcataacgc
//
LOCUS       DAJVYM010000261         2596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVYM010000261 DAJVYM010000000
VERSION     DAJVYM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.266"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..992
                     /gene="tyrR"
                     /locus_tag="OQS60_003937"
     CDS             <1..992
                     /gene="tyrR"
                     /locus_tag="OQS60_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS2389557.1"
                     /translation="TIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVEQAQKLAMLSA
                     PLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELFGHAPEGKKGF
                     FEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVRVICVTQKNLV
                     ELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQGVQRPKLAAD
                     LNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATVAVGEDAMEGS
                     LDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQKKNEE"
     gene            complement(1036..1542)
                     /gene="tpx"
                     /locus_tag="OQS60_003938"
     CDS             complement(1036..1542)
                     /gene="tpx"
                     /locus_tag="OQS60_003938"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS2389558.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            1661..>2596
                     /gene="ycjG"
                     /locus_tag="OQS60_003939"
     CDS             1661..>2596
                     /gene="ycjG"
                     /locus_tag="OQS60_003939"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS2389559.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPAL"
BASE COUNT          629 a          618 c          750 g          599 t
ORIGIN      
        1 caacgattcg tatgggccgc cagttgcaaa atgtcgccgc ccaggacgtc agcgccttca
       61 gtcaaattgt cgccgtcagc ccgaaaatga agcatgttgt cgaacaggcg cagaaactgg
      121 cgatgctaag cgcgccgctg ctgattacgg gtgacacagg tacaggtaaa gatctctttg
      181 cctacgcctg ccatcaggca agccccagag cgggcaaacc ttacctggcg ctgaactgtg
      241 cgtctatacc ggaagatgcg gtcgagagtg aactgtttgg tcatgctccg gaagggaaga
      301 aaggattctt tgagcaggcg aacggtggtt cggtgctgtt ggatgaaata ggggaaatgt
      361 caccacggat gcaggcgaaa ttactgcgtt tccttaatga tggcactttc cgtcgggttg
      421 gcgaagacca tgaggtgcat gtcgatgtgc gggtgatttg cgttacgcag aagaatctgg
      481 tcgaactggt gcaaaaaggc atgttccgtg aagatctcta ttatcgtctg aacgtgttga
      541 cgctcaatct gccgccgcta cgtgactgtc cgcaggacat catgccgtta actgagctgt
      601 tcgtcgcccg ctttgccgac gagcagggcg tgcagcgtcc gaaactggcc gctgacctga
      661 atactgtact tacgcgttat gcgtggccgg gaaatgtgcg gcagttaaag aacgctatct
      721 atcgcgcact gacacaactg gacggttatg agctgcgtcc acaggatatt ttgttgccgg
      781 attatgacgc cgcaacggta gccgtgggcg aagatgcgat ggaaggttcg ctggacgaaa
      841 tcaccagccg ttttgaacgc tcggtattaa cccagcttta tcgcaattat cccagcacgc
      901 gcaaactggc aaaacgtctc ggcgtttcac ataccgcgat tgccaataag ttgcgggaat
      961 atggtctgag tcagaagaag aacgaagagt aagcgcgaat atgcctgatg gtgcaacacc
     1021 atcaggcata ttaacttatg ctttcagtac agccagagct gcttcgtaat ccggctcggt
     1081 ggtgatttca tccaccagct ggctgaaaat cacattgtca ttttcgtcaa taaccacaac
     1141 ggcacgcgct gccagacctt tcagtgggcc atcagcaatt gccacaccgt aagcttgcag
     1201 aaattcagcg ttacggaaag tggagagggt gataacgttg ttcagacctt ctgcgccgca
     1261 gaaacgagac tgggcgaacg gcagatcggc agagatacac agcacaacgg tgttgtcgat
     1321 ctcagttgcc agttggttaa acttgcgtac tgatgcggcg caaacatcgg tatcaatact
     1381 cgggaaaatg ttcagcactt tgcgtttacc cgcaaactga ccgagggtga cgtcagacag
     1441 atcttttgcc acgagagtaa aagtctgcgc tttgctaccc gcctgcggga tggaattggc
     1501 gactgtaacc gggttgccct ggaaatgaac ggtttgtgac atgattatct ttcctgttta
     1561 catatagtta acgtcacacc tagtttatgc taactgtcaa taacacagca aacgctattt
     1621 gcgcttaatc cgcagaccac cgcgacaaca aggagtaaag atgagaaccg ttaaggtatt
     1681 tgaggaagcc tggcccttac ataccccgtt tgtgattgcc cggggaagtc gcagtgaagc
     1741 gcgcgtggtg gtggttgaac tggaagaaga gggtattaaa ggcaccggcg aatgcacgcc
     1801 gtatccgcgt tatggagaaa gtgatgcctc ggtaatggcg caaattatga gcgtcgtgtc
     1861 gcaattagag aaagggctga cacgggagga gttgcaaaaa attctccctg ccggcgcagc
     1921 acgtaatgcg ctggattgtg cattgtggga tctggccgcg cgaaaacagc agcaatcgct
     1981 ggctgatttg atcggcataa cgcttcccga gacagttatc actgcacaga cggtagtcat
     2041 cggtacgcct gatcagatgg ccaatagtgc atcaacactc tggcaggcag gcgcgaaatt
     2101 actgaaagtg aagctggata accatcttat tagtgagcgg atggtggcaa ttcgcacagc
     2161 tgtgcccgat gcgacgctga tcgttgatgc aaatgaatcc tggcgtgcag aaggggtggc
     2221 ggcgcgttgc cagctattgg cggatttagg cgttgcgatg cttgaacaac cccttcctgc
     2281 gcaggatgat gcggcgctgg agaattttat tcatccgttg ccgatttgtg ctgatgagag
     2341 ttgtcataca cgtagcaatc tgaaggcgct gaaagggcgc tatgagatgg ttaacattaa
     2401 gctcgataaa atcggtggtc tgacggaagc gctggcgctg gcgactgaag cgcgtgcaca
     2461 aggtttcagt ctgatgctgg gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt
     2521 accgctggtg ccgcaggtca gtttcgccga tcttgacgga ccgacctggc tggcggtaga
     2581 tgtggaaccg gcgctt
//
LOCUS       DAJVYM010000262         2572 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVYM010000262 DAJVYM010000000
VERSION     DAJVYM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.267"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..392
                     /locus_tag="OQS60_003940"
     CDS             <1..392
                     /locus_tag="OQS60_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCS2389560.1"
                     /translation="CIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGL
                     GISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWF
                     FLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            389..868
                     /locus_tag="OQS60_003941"
     CDS             389..868
                     /locus_tag="OQS60_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCS2389561.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(922..1872)
                     /gene="corA"
                     /locus_tag="OQS60_003942"
     CDS             complement(922..1872)
                     /gene="corA"
                     /locus_tag="OQS60_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS2389562.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2242..>2572
                     /locus_tag="OQS60_003943"
     CDS             2242..>2572
                     /locus_tag="OQS60_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389563.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          750 a          589 c          559 g          674 t
ORIGIN      
        1 aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct cttatcctgc
       61 aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac ttcgaggaca
      121 gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata tctcttttaa
      181 gttttcaaaa atacgctata ggatggagtg caacaattca ttttgtagca caggaccact
      241 tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga ttttttcgca
      301 tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc accaatttta
      361 acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa ttatattatg
      421 tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac agattttgta
      481 tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc catggactga
      541 cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc aacatcccct
      601 accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct tcactgacta
      661 taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa
      721 tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt atcgtaacac
      781 tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata aaatagagcg
      841 agttttcaat ccgcatgact ttaaataaga caatttttag aaataagccg gataaaaaaa
      901 tagctttatc cggcactcat attacagcca gttcttgcgc ttaaagtaca gatacggtgc
      961 caggcccgcg aggatcataa agataatcgc gcctgggtag ccgaaactcc acttcagttc
     1021 tggcataaac tcaaagttca tcccatagct ggaagcaacg agcgtcggcg gcaggaatac
     1081 cacggatacc accgagaaga ttttgatgat gcggttctgc tcgatgttga taaaacccat
     1141 cgccgcctgc atcaggaagt tcaccttctg gaacagggat tcgttatgcg gcagcaggga
     1201 ttcgatatct cgcaggattt cacgcgcctg ctccagttgc ccacccggta aacgcgcttt
     1261 acgcaccagg aagttgagcg cgcgctgggt atccatcaga cacaggcgaa ctttccagcc
     1321 gatatcttcc agttccgcca gagtggagag cgcctcgtcg tactcatcgc cctgatgccc
     1381 ttccataatc acccggctca actgctccag gtcgctatag atattttcaa tttcatctgc
     1441 caactgttcg attttggttt cgaacagatc cagcagcagc tcgtaggcgt taccgtctac
     1501 catcgactgg ctacgggcac gcatacgata cagacgaaaa gcgggcagtt cacgctcacg
     1561 cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac cggcgtgatc
     1621 ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa
     1681 acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct ggccaagttc
     1741 agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt
     1801 tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt ccagttgaaa
     1861 tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac
     1921 agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg gaaaaattga
     1981 ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag ttgatcctgg
     2041 atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt
     2101 gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca gaacaccaca
     2161 gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca ggataaaaaa
     2221 atccaacatc caggacctat aatggcgcat cggctactca ttggtaaagg aatgatcacc
     2281 ttgaatctca aacggatttt tctcgccctc accttacttc ccttatttgc tgttgccgct
     2341 gatgattgcg cactctcaga tccgatgctg accgtacagg cgtataccgt taatcctcaa
     2401 acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg aactttacat
     2461 gctctgctgg tggatatgaa tagtcagggt caggtgcaga tggcgatgaa cggcggcatc
     2521 tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca ga
//
LOCUS       DAJVYM010000263         2568 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVYM010000263 DAJVYM010000000
VERSION     DAJVYM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.268"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            186..1139
                     /locus_tag="OQS60_003944"
     CDS             186..1139
                     /locus_tag="OQS60_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2389564.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1180..2175)
                     /gene="pgl"
                     /locus_tag="OQS60_003945"
     CDS             complement(1180..2175)
                     /gene="pgl"
                     /locus_tag="OQS60_003945"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS2389565.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2435..2566)
                     /locus_tag="OQS60_003946"
     CDS             complement(2435..2566)
                     /locus_tag="OQS60_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS2389566.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
BASE COUNT          711 a          631 c          577 g          649 t
ORIGIN      
        1 ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat taagggcaaa
       61 gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt taagttggct
      121 aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca ttttccggag
      181 taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca gagtccagtt
      241 cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg cgaagaataa
      301 aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc aaggttacgt
      361 taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag aaattcgatg
      421 aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg acgttagcgt
      481 gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt aatgagctgt
      541 atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg gaatcggtgc
      601 tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca tccattgatt
      661 ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat ccattggcca
      721 aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc gtacgctcct
      781 ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg aagctggact
      841 ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg ggactgggga
      901 tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt attggtattc
      961 cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa gatgccgtgc
     1021 tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact gacgataaag
     1081 acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag ataggttaat
     1141 acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt taaccaccac
     1201 ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc cctgctcgcc
     1261 aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcgacaatca gatacttgcc
     1321 gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc cttctttact
     1381 caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg tacggtcgca
     1441 ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac aggtgtcgga
     1501 gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt gtggatcttt
     1561 cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat attgttcgtt
     1621 tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt ccgcagggtc
     1681 ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat cctgctttaa
     1741 tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc aaccgtccag
     1801 cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg ttacgctcac
     1861 gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg tggaaatatg
     1921 cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat cgtccggggc
     1981 aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat aacgtttgtc
     2041 cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg tcagcgtcag
     2101 tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc tggcgatata
     2161 aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt catacacata
     2221 acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat atcgcacatc
     2281 aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat attagtcacc
     2341 gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta tgactctccc
     2401 cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact tctggccaac
     2461 ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta cgttgtagat
     2521 cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatgg
//
LOCUS       DAJVYM010000264         2551 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVYM010000264 DAJVYM010000000
VERSION     DAJVYM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.269"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1489
                     /gene="helD"
                     /locus_tag="OQS60_003947"
     CDS             <1..1489
                     /gene="helD"
                     /locus_tag="OQS60_003947"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS2389567.1"
                     /translation="RQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWL
                     LARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVP
                     IVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQWSVPEGNFWDDEKLQ
                     RRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWKGALKAEN
                     AVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAV
                     GDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRIGKVANRFIQQNPGQL
                     KKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILILARYHHMRPASLEKAA
                     TRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPD
                     AEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP"
     gene            complement(1521..1979)
                     /gene="mgsA"
                     /locus_tag="OQS60_003948"
     CDS             complement(1521..1979)
                     /gene="mgsA"
                     /locus_tag="OQS60_003948"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS2389568.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(2075..>2551)
                     /locus_tag="OQS60_003949"
     CDS             complement(2075..>2551)
                     /locus_tag="OQS60_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS2389569.1"
                     /translation="VEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLENERE
                     ANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASLPQFA
                     TMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQPSS"
BASE COUNT          634 a          613 c          708 g          596 t
ORIGIN      
        1 ccgccaggtc gagtcttcac cgctgaatcc ggcgcaggcc cgggcagtcg ttaatggcga
       61 gcattctctg ttagtgctgg caggtgcagg aagcggaaaa acgtcggtgc tggtggcccg
      121 tgcaggctgg ttgctggcgc gtggtgaagc gtcccctgag caaattttat tgctggcgtt
      181 tggtcgcaaa gccgctgaag agatggacga gcggattcgc gaacggctac ataccgaaga
      241 cattaccgca cgcacgtttc atgcgctggc gctgcatatt attcagcagg gcagcaaaaa
      301 agttccgata gtcagcaaac tggaaaatga taccgctgcc cgtcatgaac tctttattgc
      361 tgagtggcgc aagcaatgca gcgaaaagaa agcgcaggcg aagggctggc ggcaatggct
      421 gacggaagaa atgcagtggt cagtgccaga aggtaacttc tgggatgatg aaaaattaca
      481 gcgtcgcctt gcctctcgcc tcgatcgctg ggtaagtctg atgcggatgc acggtggtgc
      541 acaggcagaa atgattgcca gtgcacccga agagattcgc gatctgttca gtaaacgtat
      601 caagttgatg gccccgttat taaaagcctg gaaaggtgcg ctgaaggcag aaaacgctgt
      661 cgatttttcg ggccttattc atcaggcgat tgtgattctg gagaaaggtc gctttatcag
      721 cccgtggaag catattctgg ttgatgaatt tcaggatatc tcgccgcagc gggcagcgtt
      781 gttagcggca ttacgcaagc aaaacagtca gacgacgttg ttcgctgttg gtgatgattg
      841 gcaggcgatt taccgattca gcggtgcgca aatgtcgctc accaccgctt tccatgaaaa
      901 ctttggtgaa ggcgaacgtt gtgatttaga cacgacttac cgttttaaca gtcgtatcgg
      961 taaggtggca aaccggttta ttcagcagaa cccaggccag ctgaaaaagc cgctaaacag
     1021 cttaaccaat ggagacaaaa aagccgtcac gttattggat gagagtcaac ttgacgcttt
     1081 gctggataag ctctctggtt atgccaaacc ggaagagcgc attctgatcc tggcgcgtta
     1141 ccatcacatg aggcctgcca gcctggaaaa agcggcaaca cgctggccga agttgcaaat
     1201 cgactttatg accattcatg ccagcaaagg gcaacaggcg gattacgtca tcatcgttgg
     1261 cttgcaggag ggaagtgatg gttttccggc tgcggcgcgg gagtcgatta tggaagaggc
     1321 gctactgcca ccggttgagg atttcccgga cgctgaagaa cggcggttaa tgtacgtggc
     1381 gctgacccgg gcacgccatc gggtatgggc actgtttaac aaagagaatc cctctccctt
     1441 tgtggaaata ctgaaaaatc tggatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg
     1501 cgtttgccac ctgtgcaata ttacttcaga cggtccgcga gataacgctg ataatcgggg
     1561 attagaatat cgaccgcgtc gttgaaatgc ggcgactgga ttatgaagtc tgccgttgcc
     1621 acgttggtgg cgaccggaat gttccatacc gtcgccagac gcagcaaggc tttcacgtca
     1681 ggatcgtgcg gcacggcatt tagtggatcc cagaagaaaa tcaatacatc aattttccct
     1741 tctgagatca atgcgccaac ctgctggtca ccccccattg ggccactcaa catcgcgttg
     1801 acgttcatgc cggtcgcgcg ggaaattaag ttaccggtag tgcctgttgc atacagtacg
     1861 tgttgttcca gtaacggttg atgccgttcc acccagctca tcagcatttg tttgcagtga
     1921 tcgtgtgcca ccagcgcaat atgtttccgc gcaggtaaag tgcgagtcgt cagttccata
     1981 atgtacatcc gtagttaact ttcctacaga ttactgtaag cacttatcgc tgcaagataa
     2041 agaccgaaaa agcctgcgca caggcacaaa aatctcagga agatggttgt ttttccgccc
     2101 actgcaggaa agtatttcgc gtttgtgggt cagccagttt aaaccaatac ttcagccgtt
     2161 gttctgtgag cacctgagac tgcggtggaa tagcatccag ctcggaaaca cccgaaagca
     2221 gcgtactgtc atctgccatc atcgtggcaa attgcggtag tgaagcgcgt ttcgcggatt
     2281 tgttatagcg ttcaacctcg acctcataat caatcgtttt tgccgttgag gtaatggcga
     2341 gaatatccag ctttaccgga atcggcgtcg catcgccatc caacaattca aggcgcggcg
     2401 cggcatcaaa atggttagct tcccgctcat tctccaggcg aggcaggcga aaattgacct
     2461 ggttgatgag ctgggtatta aaactgacca ccagcggtgg ggagatatac agccgttctt
     2521 cactattgga aagatgaatc gtcttctcaa c
//
LOCUS       DAJVYM010000265         2425 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVYM010000265 DAJVYM010000000
VERSION     DAJVYM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.270"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..506
                     /gene="mhpC"
                     /locus_tag="OQS60_003950"
     CDS             <1..506
                     /gene="mhpC"
                     /locus_tag="OQS60_003950"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCS2389570.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
     gene            516..1325
                     /gene="mhpD"
                     /locus_tag="OQS60_003951"
     CDS             516..1325
                     /gene="mhpD"
                     /locus_tag="OQS60_003951"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCS2389571.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            1322..2272
                     /gene="mhpF"
                     /locus_tag="OQS60_003952"
     CDS             1322..2272
                     /gene="mhpF"
                     /locus_tag="OQS60_003952"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCS2389572.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            2269..>2425
                     /locus_tag="OQS60_003953"
     CDS             2269..>2425
                     /locus_tag="OQS60_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCS2389573.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGL"
BASE COUNT          547 a          616 c          717 g          545 t
ORIGIN      
        1 tggcgttcac ccttaactgg ccggagcgcg tcggcaaact ggtgctgatg ggcggcggta
       61 cgggcggcat gagtttgttt acgccgatgc caaccgaagg tattaagcga ctgaatcagc
      121 tttatcgtca gccgactatc gaaaacctga agctgatgat ggatatcttc gttttcgata
      181 ccagcgattt gaccgacgcc ctgtttgaag cgcgcctgaa taatatgctg tcgcgccgcg
      241 atcacctgga aaacttcgtt aagagcctgg aagctaatcc gaaacagttc ccggattttg
      301 gcccacgtct ggcggaaatc aaagcgcaaa ccctgattgt ctgggggcgc aacgaccgct
      361 ttgtgccgat ggatgcgggt ctgcgtctgc tgtccggcat tgccggttct gaactgcata
      421 tcttccgcga ctgtggtcac tgggcgcagt gggaacatgc cgacgctttc aatcaattgg
      481 tgctgaattt cctcgcacgc ccttaaggaa tggtcatgac gaagcatact cttgagcaac
      541 tggcggcgga tttacgccgc gccgcagagc agggcgaagc gattgcgccg ctgcgcgatc
      601 tgattggtat cgatcacgct gaagcggcct acgccattca gcacataaat gtgcaatatg
      661 acgttgtgca ggggcgtcgc gtggtagggc gtaaagtggg cctgacgcat ccgaaagtgc
      721 aacaacaact gggcgttgat caaccggatt ttgggacgtt atttgccgac atgtgttatg
      781 gcgataacga aatcattcct ttttcccgtg ttctgcaacc ccgcattgaa gcggagatcg
      841 cactggtgtt gaaccgcgat ttacccgcaa ctgatatcac cttcgacgaa ctgtataacg
      901 ccattgaatg ggtacttccg gcgctggaag tggtggggag ccgcattcgc gactggtcga
      961 ttcagtttgt cgataccgtg gcagataacg cttcctgcgg ggtgtatgtc atcggcggtc
     1021 cggcgcaacg tccggcgggg ttagacctga aaaactgcgc catgaagatg acgcgtaata
     1081 acgaagaggt ttccagcggg cgcggcagcg aatgcctggg acatccgctt aatgccgccg
     1141 tctggctggc acgcaaaatg gccagtctgg gtgaaccgct gcgcgccgga gatatcattc
     1201 ttaccggggc attaggcccg atggtggcgg tgaatgcggg cgatcgtttt gaagcccata
     1261 ttgaaggcat aggttcagtt gctgcgacat tttcaagcgc agccccaaaa ggaagtctgt
     1321 catgagtaag cgtaaagtcg ccattatcgg ttctggcaac attggcaccg atctgatgat
     1381 taaaattttg cgtcacggtc agcatctgga gatggcggtg atggtcggca ttgatcctca
     1441 gtccgacggt ctggcgcgtg ccagacgtat gggcgtcgcc accacccatg aaggggtgat
     1501 cgggctgatg aacatgcctg aatttgctga tatcgacatt gtatttgatg cgaccagcgc
     1561 gggcgcgcat gtgaaaaacg atgccgcttt acgcgaagcg aaaccggata ttcgcttaat
     1621 tgacctgacg cctgctgcca tcggccctta ctgcgtgccg gtggttaacc tcgaggcgaa
     1681 cgtcgatcaa ctgaacgtca acatggtcac ctgcggcggc caggccacca ttccaatggt
     1741 ggcggcagtt tcacgcgtgg cgcgtgttca ttacgccgaa attatcgctt ctatcgccag
     1801 taaatctgcc ggacctggca cgcgtgccaa tatcgatgaa tttacggaaa ccacttcccg
     1861 agccattgaa gtggtgggcg gcgcggcaaa agggaaggcg attattgtgc ttaacccagc
     1921 agagccaccg ttgatgatgc gtgacacggt gtatgtattg agcgacgaag cttcacaaga
     1981 tgatatcgaa gcctcaatca atgaaatggc tgaggcggtg caggcttacg taccgggtta
     2041 tcgcctgaaa cagcgcgtgc agtttgaagt tatcccgcag gataaaccgg tcaatttacc
     2101 gggcgtgggg caattctccg gactgaaaac agcggtctgg ctggaagtcg aaggcgcagc
     2161 gcattatctg cctgcctatg cgggcaacct cgatattatg acttccagtg cgctggcgac
     2221 agcggaaaaa atggcccagt cactggcgcg caaggcagga gaagcggcat gaacggtaaa
     2281 aaactttata tctcggacgt cacattgcgt gacggtatgc acgccattcg tcatcagtat
     2341 tcgctggaaa acgttcgcca gattgccaaa gcactggacg atgcccgcgt ggattcgatt
     2401 gaagtggccc acggcgacgg tttgc
//
LOCUS       DAJVYM010000266         2417 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVYM010000266 DAJVYM010000000
VERSION     DAJVYM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.271"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..160)
                     /locus_tag="OQS60_003954"
                     /pseudo
     CDS             complement(1..160)
                     /locus_tag="OQS60_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(162..2417)
                     /locus_tag="OQS60_003955"
                     /pseudo
     CDS             complement(162..2417)
                     /locus_tag="OQS60_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          570 a          683 c          515 g          649 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttaccg
     1621 gaggcatttc ctccgataaa tccatagccc gttgaattaa gggtggcacc attactgaca
     1681 tttacgatac cgctaccaaa attaccgaca ttgataccca cagtatcgaa cgatgatgtc
     1741 gcatccatca ggttcagtgt gccgataccg cttgctttat cgcccaaaaa agtaatattt
     1801 tgcgatacaa acttaccgcc atcgctaata ttgaggtttc cgacgcccgc aacaccaatg
     1861 gtggtataga cattcttatt gacctcccac atcccccccg tggtgatgct gacattcccg
     1921 acgccgtttt ctccataacc gagtgagctt cctccgttgc tgacaacaag cccctcatta
     1981 ctgatattca tttcgccgtg gccgtcataa ccaagattag ttccgagatt agtgataacg
     2041 gagttcttat ccttaacagt aatgttgcct gtgcctgttt ctttcatccc cgcagtgata
     2101 attcccgaat ctactttacc ttcacgcgag acattaagtt caccgtcacc agcatcaccg
     2161 atatagatat agcggaaagc ttcgcctgtt ccgaggaaac tccaatgccc tttatcggtc
     2221 acgttgacga caccgtggga accgtcctga acgcccacca atgaatattc tttgttatta
     2281 atcagaccat tattggaaat attgactgtg ccattaccga aatacccatt atagagtctg
     2341 cgtaccgtga tgacagagtc ttgatcctgc acattcaggg ttccgatacc ggtggcattg
     2401 ccaccgataa tcgtatt
//
LOCUS       DAJVYM010000267         2415 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVYM010000267 DAJVYM010000000
VERSION     DAJVYM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.272"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(53..469)
                     /gene="rraB"
                     /locus_tag="OQS60_003956"
     CDS             complement(53..469)
                     /gene="rraB"
                     /locus_tag="OQS60_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS2389574.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            631..1635
                     /gene="argF"
                     /locus_tag="OQS60_003957"
     CDS             631..1635
                     /gene="argF"
                     /locus_tag="OQS60_003957"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS2389575.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(1707..>2415)
                     /locus_tag="OQS60_003958"
     CDS             complement(1707..>2415)
                     /locus_tag="OQS60_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2389576.1"
                     /translation="YINDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKD
                     FSCDQSKIKIAETLFFNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRL
                     FDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVY
                     GDEEKTKEIVNKLPGYVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKL
                     NPPEDLRIAIKKLGWNN"
BASE COUNT          680 a          558 c          481 g          696 t
ORIGIN      
        1 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
       61 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      121 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      181 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      241 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      301 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      361 ggtgttcgat ggtgtacaac gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      421 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      481 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
      541 ataaagattt atgctttaga cttgcaaatg aataatcatc catataaatt gaattttaat
      601 tcattaaggc gttagccaca ggagggacct atgtccgggt tttatcataa gcatttcctg
      661 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
      721 aaagccgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
      781 atcttcgaaa aagactcgac ccgtacccga tgctctttcg aagttgccgc atatgaccag
      841 ggggctcgtg ttacttatct cggcccaagc ggcagccaaa ttggtcataa agagtcgatt
      901 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
      961 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1021 aatgagtttc accccacgca gctgctggcg gatctcctca ccatgcagga gcatttgccc
     1081 ggcaaaacgt tcaacgaaat gacgctagtc tatgcaggtg acgcacgtaa caacatgggc
     1141 aattcgatgc tcgaagctgc agcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1201 gcatgctggc cggaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1261 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1321 accgatgtat gggtgtcgat gggtgaagca aaagagaaat gggctgaacg cattgcattg
     1381 ctgcgtgatt atcaagtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1441 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1501 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga atctgccgcc
     1561 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     1621 acgctcagta aataattgat atcgctataa agatgccggg aaatacaaaa acatcccggc
     1681 gtcacattat tttatgcagt gatagttcaa ttattccacc ccaatttttt tattgctata
     1741 cgtaaatctt ccggaggatt taattttcct aactgttctt cattaatata tccatatata
     1801 ttgattttgc cattccggag gattttcatc attcttgttc taatttttaa ttcattaccc
     1861 atcttttctt caaaaatctt aacgacataa cccggaagtt tatttacaat ttcttttgtt
     1921 ttttcttcat caccataaac atatcctaac tcattaagat acagctgttc gccctcataa
     1981 cacttaagta attgatagaa gtaccactta gcaaaatcag gttgcttcat gaaaaaatcc
     2041 ttcaaaaaaa tatcatttac taaaatgctg acaggcactt cattaaatag taacttaaca
     2101 tcatcaaata accttagtat taagcgtgat ttttcttgat tgcttaagct aaaatcattg
     2161 acatatgcaa ccaacttatc ccaaatcaat tcctgtaatt tacggctatt tttatagggt
     2221 tctttatcga gattgaaaaa taaggtctca gctattttaa ttttagattg atcgcagcta
     2281 aaatctttag attgatcgca gctaaaatca atatgattta tcacggagca tgcatacttt
     2341 atcttttcag aatcaccaaa atattgcatt cttttattaa aacaccaatc tagtattctt
     2401 acatcattaa tatat
//
LOCUS       DAJVYM010000268         2318 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVYM010000268 DAJVYM010000000
VERSION     DAJVYM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.273"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..735
                     /locus_tag="OQS60_003959"
     CDS             <1..735
                     /locus_tag="OQS60_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCS2389577.1"
                     /translation="VILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGIT
                     LDPGLAPRAVWPTDSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQ
                     AVVAVGGSTERQSDIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQE
                     LRRTADDRLFFQLTSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASR
                     ETDILDRNAISHPGFVPVRALRVPVR"
     gene            732..1814
                     /locus_tag="OQS60_003960"
     CDS             732..1814
                     /locus_tag="OQS60_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS2389578.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            1792..>2318
                     /locus_tag="OQS60_003961"
     CDS             1792..>2318
                     /locus_tag="OQS60_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS2389579.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQP"
BASE COUNT          535 a          579 c          692 g          512 t
ORIGIN      
        1 gtcattctga tgccgccgtc ggttgccagt gattttgtgc atacgttgca ggatacctgg
       61 cgggccggaa cccggctggt ggataacaca caggatctgg tgcagtccat aacgacaatg
      121 tccggcatta cgctggaccc aggactggca ccgcgtgcgg tgtggcccac agattccgca
      181 tcggttatca ggcaaaaaca gcagacaaac cttgtggctg ctgtcatccg gacgacggca
      241 atcagcgaag ccacaagggc ggtcacttca ctgccacaac ccggaagcct ggtgaaaaat
      301 cagcaggcgg ttgtggctgt tggtggttcc actgaacgtc agtccgatat tattcacgtt
      361 tctcatccgg cacttgacag cgtggcagcc agcacaggac aggatgagag agcgcaacca
      421 cccacgcggg aaaatctcac catcatacgc aaatcgctga acgcggcgat tgaacaggag
      481 ctcaggcgta cggcggatga caggctgttt tttcagctga cgtcattacg tacagaactg
      541 aaccgggata ttcaggcgcg tctggttcag acggaggaaa ccgcagagcg aacgccagcg
      601 gaagttctgc ctgcgcttgt tctggctgcg tcatggtacg acgatgcgtc ccgtgaaact
      661 gatatcctgg atcgaaatgc catctctcat ccgggctttg ttccggtcag ggcattaagg
      721 gtacccgtca gatgaatgat accgttttac ttcgggtttc cggtcgcgaa tggggcggct
      781 ggacatccgt ccgtatcagt gcgggcatta accgtattgc ccgggatttt aatgttgcca
      841 ttaccacccg ctggcccgga agccgggatt atcaaccccg gataaaaaat ggtgagctgg
      901 ttgaagtgct tatcggggat gagcctgtgc tcaccggata tgtggaggca ctaccgcttc
      961 gttatgacgc cagcagcgtc agcatgggaa ttgtcgggcg aagcaaaaca gccgatctgg
     1021 ttgactgctc tgctttgcca ctccagcaga gcggaaaaaa cctgctcaga atagtcagtg
     1081 aactggctgc gccatttggc atcaccgttg ttgatgctgg cgtgccgcag acagcggtga
     1141 ttgatgccca gccggaacac ggcgaaaccg ttgccgattg tcttaaccgg ttactggggc
     1201 aggttcagac gctggcttat gacgatgaat gcgggcgact ggttctggga aaacccggaa
     1261 caggtaaagc ggcgacggcg ctggtgctgg tgctgggaga gaatattctt tcctgtgaca
     1321 cggaaagaag catcagagag cggttttctg aatatcaggt cagtgggcag cgcccgggca
     1381 acgacgatga ttttggtgag gccaccattg ccgcaatacg tcagaccatt caggacagtg
     1441 gcgtgacccg ttatcgccct ttgttgattc agcagtcagg cacagcaacg acagcaacct
     1501 gtaaggcccg ttgtgaattt gaagcgcgcc aacgggctgc gcttacccgt gagacaacat
     1561 atacggttca gggctggcgg cagggcagtg gcgcgttatg gcgtccgggg ttatctgtca
     1621 tcgttttcga cccgctgaat aattttgata atgatgaact ggtgatcgca gaagttacct
     1681 ataaccagga cgaccggggc acgacgactg aattacgggt tggcccggca gatgcttatc
     1741 tccccgagcc tgttaccgcc aggaagaaaa aaatgttgag gaggatttct gatgaacggg
     1801 ttttctcttc gtaatctgat ttcccgggct gtcatcacgg cggtggattc cgccagaaag
     1861 tgtcagtctg tagggttgaa aatgatagcc ggagatcaga aacaacacgt tgagcacctt
     1921 gaaccttatg gttttacatc tgccgcacag aacggtgctg agggcgttgc tttatttccg
     1981 gcgggcgatc gttctcatgg tgtggttgtg gtcgtggctg acagacgtta ccggctgaaa
     2041 ggactgaaac gtggggaagt ggcgctttat gacgatctgg gacagtccgt tgtcctgacc
     2101 cgttccggta ttgtggtgga cggggccggg aaggccataa tttttaaaaa cgcgcctaaa
     2161 gcgcgctttg aaatgccagt cgaatccacc gccgatatta ctgacaattg cgacagtggc
     2221 ggactcagca tgcagcaaat gcggcaggcc tacaatgccc acaaacatac cgaaaatggt
     2281 gatggtggcg ggatcactga cacgccggat caaccgat
//
LOCUS       DAJVYM010000269         2295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVYM010000269 DAJVYM010000000
VERSION     DAJVYM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.274"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..264
                     /locus_tag="OQS60_003962"
     CDS             <1..264
                     /locus_tag="OQS60_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2389580.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            434..712
                     /locus_tag="OQS60_003963"
     CDS             434..712
                     /locus_tag="OQS60_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCS2389581.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            712..1089
                     /locus_tag="OQS60_003964"
     CDS             712..1089
                     /locus_tag="OQS60_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389582.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1558..>2295
                     /locus_tag="OQS60_003965"
     CDS             1558..>2295
                     /locus_tag="OQS60_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2389583.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDE"
BASE COUNT          758 a          357 c          549 g          631 t
ORIGIN      
        1 gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg
       61 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa
      121 ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa ggtattcaag
      181 gcagggttaa gacgtaagat gcgaaaagca gccatggata gaaactatct cgcgtcagtc
      241 cttgcgggga gcgggctttc gtaatctttc cctagggagt cagccgaatg ttgggaagga
      301 tttgacggat gaagagaaga actgcttggc cggcggcagt acgggaagcc cgggaggatg
      361 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      421 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      481 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      541 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      601 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      661 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      721 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      781 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      841 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      901 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      961 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
     1021 ttaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1081 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1141 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatag tgacgtgtgg
     1201 caatggctat tagatgttat ggtgtgatgg gctttggtga tttaagagct aagttagatt
     1261 gccttatgaa aaagtatggt tatatattta atgatgtgta tcatatattt aaataaaaaa
     1321 cggttctctc ttgtcaagat agtaatagga tagactattg tgttgataaa tataattaag
     1381 agtctacttt gttgatttta gaagactttg gagcagggaa agatcatcgg aagtacactt
     1441 tttgtactaa ataattcgcg ctaaatgttc aataattgag atattcctta ttacctaaaa
     1501 ctatttttca tagcttatat atgatcaaat actccttgca taaataagga gaacaaaatg
     1561 gaacttaaaa aactgatgga acatatttct attatccccg attacagaca agcctggaaa
     1621 gtagaacata aattgtcaga tattctactg ttgactattt gtgccgtcat ttctggtgca
     1681 gagggttggg aagatataga ggattttggt gaaacacatc tcgatttttt gaagcaatat
     1741 ggtgattttg aaaatggtat tcctgttcac gatactattg ccagagttgt atcctgtatc
     1801 agtcctgcaa aatttcacga gtgctttatt aactggatgc gtgactgcca ttcttcagat
     1861 gataaagacg tcattgcaat tgatggaaaa acgctccggc actcttatga caagagtcgc
     1921 cgcaagggag cgattcatgt cattagtgcg ttctcaacaa tgcacagtct ggttctcggg
     1981 cagattaaga cggatgagaa atccaatgag attacagcca ttcctgaact tcttaacatg
     2041 ctggatatta aaggaaaaat catcacaact gatgcgatgg gatgccagaa agatattgca
     2101 gagaagatac aaaaacaggg cggtgattat ttattcgcgg taaaaggaaa ccaggggcgg
     2161 cttaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc agagcatgac
     2221 agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa tccgtcttca tattgtttgc
     2281 gatgtccctg atgaa
//
LOCUS       DAJVYM010000270         2286 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVYM010000270 DAJVYM010000000
VERSION     DAJVYM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.275"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="OQS60_003966"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="OQS60_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCS2389584.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="OQS60_003967"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="OQS60_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCS2389585.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="OQS60_003968"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="OQS60_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS2389586.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..>2286
                     /gene="cas6e"
                     /locus_tag="OQS60_003969"
     CDS             2131..>2286
                     /gene="cas6e"
                     /locus_tag="OQS60_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS2389587.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
BASE COUNT          569 a          570 c          613 g          534 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcgggaa
     2281 gaactc
//
LOCUS       DAJVYM010000271         2278 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVYM010000271 DAJVYM010000000
VERSION     DAJVYM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.276"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..2103
                     /locus_tag="OQS60_003970"
     CDS             <1..2103
                     /locus_tag="OQS60_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2389588.1"
                     /translation="IIPGTAAEFDFNHQRLNLSIPQIALYRDARGYVSPSRWDDGIPT
                     LFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTRNDQTSSWNTISS
                     YLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQRGFAPTVRGIAN
                     SSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESDGTQRRFIQPYSS
                     LPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFTLYGGLLGSEDYY
                     ALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDIPETNTNIAVSYY
                     RYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTIFDGVSLYASGSQQDYWGNNDKN
                     RNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSLNLSIPLERWLPRSRVSYQMTSQ
                     KDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGN
                     DSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAV
                     VPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVIVSD
                     RNGKPLPFGALASNDETGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFS
                     TPDSEPTTSVLQGTAQCH"
     gene            2114..>2278
                     /locus_tag="OQS60_003971"
     CDS             2114..>2278
                     /locus_tag="OQS60_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2389589.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          626 a          554 c          536 g          562 t
ORIGIN      
        1 atcattcccg gtacagcagc tgaatttgat ttcaatcatc agcgacttaa tttgagcatt
       61 ccccaaattg cactgtaccg tgatgcaaga ggttacgtct ccccttctcg ttgggacgat
      121 ggtataccaa cgctgtttac caactactcg tttacaggtt ctgataaccg ttaccgccag
      181 ggcaatcgta gccaacgaca gtacctgaat atgcaaaatg gtgctaattt tggcccctgg
      241 cgattacgca actattccac atggacacgc aacgatcaga catcaagctg gaataccatc
      301 agtagttatt tacaacgtga tatcaaggca ttgaagtctc agttgcttct gggagaaagc
      361 gccaccagcg gcagtatttt ttccagctac acctttactg gcgttcaact cgcttccgac
      421 gataatatgt tgccaaacag ccagcgcgga tttgccccaa cggtacgcgg tatcgcaaac
      481 agtagtgcaa tcgtgactat caggcaaaat ggttatgtga tctatcaaag caacgtgcca
      541 gcgggtgcct ttgaaattaa cgatctctac ccctcttcca acagcggcga tttagaagtc
      601 acgattgaag aaagtgacgg tacgcaacgt cgctttatcc agccttattc ttcattaccc
      661 atgatgcagc gacctgggca tctaaaatat agcgcgaccg ctggacgcta tcgcgctgat
      721 gcaaacagtg atagcaaaga acccgaattt gctgaagcca cggcaatata tggtttgaat
      781 aatactttta cgctgtatgg tggcctgctc ggttctgaag attattatgc gctggggatc
      841 ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga tggatatcaa cagagctgac
      901 acccaattcg ataaccagca ctcttttcat ggctatcaat ggcgtacgca gtacatcaaa
      961 gatatcccgg aaaccaacac caatatcgct gtcagctact atcgctatac caacgatggc
     1021 tattttagtt ttgatgaagc caatacccgt aattggaact ataacagtcg ccaaaaaagt
     1081 gaaattcaat tcaacatcag ccagacaata tttgatgggg taagtctgta tgcctccggt
     1141 tcgcagcaag actattgggg caataacgat aaaaacagga atatctcagt tggggtttcc
     1201 ggtcagcaat ggggaattgg ttacagcctg aattatcaat acagtcgcta cacggatcaa
     1261 aataatgacc gcgcactctc tttgaatctc agtattccgt tagaacgctg gttaccgcgt
     1321 agccgggttt cctatcagat gaccagccag aaagatcgcc caacccaaca tgaaatgcgt
     1381 cttaatggct cactgctgga tgatggtcgc ctgagctata gcctggaaca aagtctggat
     1441 gacgataaca accataacag tagcctgaac gccagttacc gttcacctta tggcaccttc
     1501 agtgccggat acagctacgg taatgacagc agccaataca attacggcgt taccggcggc
     1561 gtggttatcc atactcatgg cgtgacgctc tcgcaatatc tgggcaacgc ttttgcgctt
     1621 atcgatgcta atggggcttc tggcgtgagg atacaaaact atccggggat tgctactgat
     1681 ccctttggct atgcagtggt tccttatctc acgacttacc aggaaaaccg tctctcggta
     1741 gatactacgc agctgcccga taacgtcgat cttgagcaaa caacacagtt tgtggtgccc
     1801 aacagaggtg caatggtagc ggcgcgtttc aacgccaata tcggttatcg cgtacttgtt
     1861 atagtcagcg atcgcaacgg taaaccgttg ccctttggcg ctcttgccag caacgatgag
     1921 acggggcaac aaagtatcgt cgatgagggc ggcatactat atctctctgg gatatcgagt
     1981 aaatcacaaa gctggactgt acgctgggga aatcaggcag atcaacaatg tcagtttgct
     2041 tttagtacac cggattcaga accaacaacc tctgtattac aaggcacggc gcagtgccat
     2101 taaggataaa aaaatgaaaa gagcgcctct tataacagga cttttgttga tatccacatc
     2161 ctgcgcttat gcctcctcag gagggtgtgg agctgacagc actagcggtg cgacaaatta
     2221 cagcagtgtg gttgatgatg ttacggtgaa ccagacagat aacgtgacag gacgggag
//
LOCUS       DAJVYM010000272         2258 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVYM010000272 DAJVYM010000000
VERSION     DAJVYM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.277"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1549
                     /locus_tag="OQS60_003972"
     CDS             <1..1549
                     /locus_tag="OQS60_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCS2389590.1"
                     /translation="VASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVCIGIFKY
                     NLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRNQLLASP
                     SVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSLETLTRT
                     ITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVISDGNGVL
                     LPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVLGNDPAY
                     GMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQLHNRLS
                     SMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKDHILLAA
                     DVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDSVPVDPNML
                     NQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEVPDELEL
                     KIMGALM"
     gene            complement(1672..2169)
                     /gene="rayT"
                     /locus_tag="OQS60_003973"
     CDS             complement(1672..2169)
                     /gene="rayT"
                     /locus_tag="OQS60_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS2389591.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
BASE COUNT          532 a          599 c          634 g          493 t
ORIGIN      
        1 tgttgccagc gaggccgatt tctacggcgc gatggacggg gcatcgaaat ttgtgcgcgg
       61 ggatgctatc gccgggatga tgattctggc gatcaacctg atcggcggcg tctgtatcgg
      121 gatcttcaaa tacaacctga gcgccgacgc cgccttccag cagtatgtgc tgatgaccat
      181 cggcgacggg ctggtggcgc aaatcccttc cctgctactc tccaccgcgg cggcgattat
      241 cgtcacccgc gtcagcgaca acggcgatat cgcccatgac gtgcgtaacc agttgctggc
      301 aagcccatcg gtgctctaca ccgctaccgg aattatgttc gtactggcgg tagtgccggg
      361 aatgccacat ctgccgtttt tgctgttcag cgccctgctt ggctttaccg gctggcggat
      421 gagcaaacgc ccgcaggcgg cggaagcgga agagaaaagt ctcgaaacgc tgacccgcac
      481 catcaccgaa accagcgagc aacaggtcag ttgggaaacc attccgctga tcgagcccat
      541 cagcttaagc ctcggctaca agctggtggc gctggtggac aaagcccagg gcaacccgct
      601 cacccagcgt attcgcggcg tgcgacaggt gatatccgac ggcaacggcg tgctgctgcc
      661 ggagatccgc attcgggaaa acttccgcct taagcccagc cagtacgcta ttttcatcaa
      721 cggcattaag gctgatgaag cggatattcc ggcggataaa ctgatggcgc tgccctccag
      781 cgaaacctac ggcgagatag acggcgtgct ggggaacgac ccggcgtacg ggatgccggt
      841 gacctggatc cagcctgcgc agaaagcgaa ggcgctgaat atggggtatc aggtgatcga
      901 cagcgccagc gtgattgcta cccatgtaaa taagattgtg cgcagctata ttcctgattt
      961 atttaactat gacgatatta cgcagttgca taaccgtttg tcgtcgatgg caccgcgcct
     1021 ggcggaggat ttaagcgcgg tgctcaatta cagccagttg ctgaaagtgt accgggcgct
     1081 gctgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc gccaccgtgc tggtcgccag
     1141 tagcgcggtg accaaagatc atattctgct ggcagccgat gtgcgcctgg cgctgcggcg
     1201 cagcattacc catccgttcg ttcgcaagca ggagctgacg gtgtataccc tgaataatga
     1261 gctggaaaat ctgctgacta acctagtgaa tcaggcgcaa cagggcggta aagtgatgct
     1321 cgacagcgtg ccagtggacc cgaatatgct caaccagttc cagagcacga tgccacaggt
     1381 gaaagagcag atgaaagcgg cggggaaaga cccggtgctg ctggtgccac cgcagctgcg
     1441 ccctttgctg gcgcgttatg caaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa
     1501 cgaagtgccg gacgagctgg agttgaagat tatgggggcg ttgatgtaag tatccggcat
     1561 tggtgcacaa ctgccggatg cggcgtaaac gccttatccg gcctaccgtt caggcacaag
     1621 tttgtaggca tgataagacg cgccagcgtc gcatcaggca tctgcgcata attataaaat
     1681 gatacgctcg ccagcattta tatccgttac gtcccccgcc caatcgatgg gatataaccc
     1741 tcgcgcgaca tcgcgatgga acgttgagaa tggccaatca ctcacttgct ttacccaacc
     1801 atgctttact ggatttatat aaatataatc aacatgatgc ctataatctt tggtattgcg
     1861 gatggcgtgc tcccaaaaac gtggttgcca gatatttttc aatccacaag catgggtaaa
     1921 ttgcttttta atttcccgcc agcgagagga aaaatcatca tcgccttcag gtaatgtcca
     1981 tatgcaatgc atatgctctg gcaaaacgac ccaggcgttg atttcaaaag gcctgtctcg
     2041 cttaacttta ataatggcgt tacggagcat ctggtactgg gtggtcaaga gttggcttcg
     2101 acgattgcgt aaattcaccg tgaaaaacca ggttcccccc ttgatgtaat aacgacgata
     2161 ttcagacatg gcgactcctt tttctcaacg gtaagaagaa aaggaaaggt cggcaatgag
     2221 gtgatggaac agggcaagat tacgaaagcc cgctcccc
//
LOCUS       DAJVYM010000273         2252 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVYM010000273 DAJVYM010000000
VERSION     DAJVYM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.278"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(161..1804)
                     /gene="yeeO"
                     /locus_tag="OQS60_003974"
     CDS             complement(161..1804)
                     /gene="yeeO"
                     /locus_tag="OQS60_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS2389592.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1809..1884
                     /locus_tag="OQS60_003975"
     tRNA            1809..1884
                     /locus_tag="OQS60_003975"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1842..1844,aa:Asn,seq:gtt)
     gene            complement(1922..>2252)
                     /gene="cbl"
                     /locus_tag="OQS60_003976"
     CDS             complement(1922..>2252)
                     /gene="cbl"
                     /locus_tag="OQS60_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="LysR-type transcriptional regulator similar to
                     CysB; contains helix-turn-helix (HTH) motif; in
                     Escherichia coli this protein regulates two operons
                     consisting of ABC transporters that are part of the cys
                     regulon; regulated by CysB; DNA-binding transcriptional
                     activator for the ssuEADCB and tauABCD operons; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS2389593.1"
                     /translation="DDAFARKGLLADIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQ
                     EEENLIRLDTRHLFDANTVWLGLKRGQLQRNYVWRFLELCNAGLSVEDIKRQVMENSE
                     EEIDYQI"
BASE COUNT          683 a          611 c          462 g          496 t
ORIGIN      
        1 actcgaacca gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga
       61 atcgtgagaa cgaggcgaat attagcgatg cccacccaca atgtcaaagc ctgtttttta
      121 aatttgaaat cgtttgctga aataatctgc attttgtcgt ttattccgac acaactggct
      181 ttttttcaca cttttgcggt tcggatcgag ggtatttcca cagccaacgt ccagtaacca
      241 ttcgccagta aaacagcacg gcccgcacag cccagtcggc aaacattccc atccagacac
      301 caaccacacc ccagccaagc atgatgccca gcacataacc gacgacaacc cggcaacccc
      361 acatgctcaa catagaaacc cacatggcgt aacgggcatc gcgagcacct ttaaatccag
      421 cgggtagcac ccatgaggcg gaccaaatag gcataaataa agcatttagc caaatcagaa
      481 tcacaacgac atgtttaacc tgtggatcct gggtgtaaaa cgatgccata accccggcaa
      541 agggagccgt tagccaggcg atggccgtta atccaagagt ggaaagccag aacacatgcc
      601 gcaactgaat ctctgcttgc gctatctgcc ctacccccaa ccttcggcct gtaatgatcg
      661 tagaagcaga gccgagcgca cttccgggta agttgataag agccgcaatt gaaaacgcga
      721 taaaatttcc ggcaataaca ctggtcccca tcccggcaac gaacatttgg gttaataacc
      781 gaccactggt aaataacact gattcgacac tcgcgggaat accaatcccc atgacttccc
      841 agataatgct aaaattcagc ggtttaaaat agctctttaa cgaaatcctt agcgcaggat
      901 taaaaccaat cgccagcacc cacaaaattg caactgcgcc aatataacga gaaatggtta
      961 aacccagccc tgccccgaca aatcccagtc ccggccagga gaaaaggccg taaatcaata
     1021 tgccgctaat aataatatta agaatattca ggctaccgtt aatcaatagc ggtattttcg
     1081 tattccctgc accacgaagt gccccgctac caataagagt gatggcagct gctggataac
     1141 tgagtaccgt cagctccaga taagtcaacg ccagtgcttt aacttctgtc gtggcatcac
     1201 ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat aagcgttgcc aacagtacgg
     1261 caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt cgtcgatccc
     1321 gcttaccgag actaaatgcc acaacgacag tagtaccaag atcgatagca gcaaaaaaag
     1381 ccataatgac catattgaag ctgtccgcca atcccacgcc ggccatcgca tcttttccca
     1441 gccagctgac cagaaaagtg ctcagaaccc ccatcaacag gacacaggca ttctccatga
     1501 agataggaac agcaagcggg gttatctcgc gccagaacaa cactttgtag ctcttgcgtt
     1561 tagcgtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata ttcaaagccg
     1621 accttaattg cagaaagtga aaccacattt caaataatga gggagaatca gcaaagctgc
     1681 aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga aacggcaaac
     1741 agttggggaa tttaaaaatc gggtttgaca aaagattttt cgccgttaag atgtgcctca
     1801 acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg
     1861 gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag cgggcttttt
     1921 gctatatctg ataatcaatt tcctcttcac tgttttccat cacctgccgc ttgatatcct
     1981 caactgacag tcctgcatta caaagttcca gaaagcgcca gacatagtta cgctgaagtt
     2041 gtcctcgctt cagtcccaac cagacagtat tagcatcgaa aagatgccgc gtatccagac
     2101 ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat ccgatcccaa
     2161 gcccaagagc aacataggtt ttaatgacat cagaatcctg cgcacttaat acaatatctg
     2221 ccagcaaacc tttgcgggca aatgcgtcat ca
//
LOCUS       DAJVYM010000274         2234 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVYM010000274 DAJVYM010000000
VERSION     DAJVYM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.279"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(183..257)
                     /locus_tag="OQS60_003977"
     tRNA            complement(183..257)
                     /locus_tag="OQS60_003977"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Gly,seq:tcc)
     gene            complement(261..336)
                     /locus_tag="OQS60_003978"
     tRNA            complement(261..336)
                     /locus_tag="OQS60_003978"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Thr,seq:tgt)
     gene            complement(339..415)
                     /locus_tag="OQS60_003979"
     tRNA            complement(339..415)
                     /locus_tag="OQS60_003979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Arg,seq:tct)
     gene            complement(417..493)
                     /locus_tag="OQS60_003980"
     tRNA            complement(417..493)
                     /locus_tag="OQS60_003980"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Ile,seq:cat)
     gene            complement(683..1246)
                     /locus_tag="OQS60_003981"
     CDS             complement(683..1246)
                     /locus_tag="OQS60_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS2389594.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1350..1568)
                     /locus_tag="OQS60_003982"
     CDS             complement(1350..1568)
                     /locus_tag="OQS60_003982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389595.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1727..2113)
                     /locus_tag="OQS60_003983"
     CDS             complement(1727..2113)
                     /locus_tag="OQS60_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2389596.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
BASE COUNT          576 a          595 c          498 g          565 t
ORIGIN      
        1 atcatcagtc ccctgccaga ggttcactac gtaacctgac cgacaatgca ccattctggt
       61 gctgaaaaaa gagcactgga actgcaacaa aacaacatca cagaacagga gatggtaagg
      121 agtcagacat tcccacacaa tattgtgtca gtgcaaataa caaccttcgt ctcaaatctc
      181 gctggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg aggtaatagc
      241 cattatacga tgcccgcata tggtgccgac taccggaatc gaactggtga cctactgatt
      301 acaagtcagt tgctctgcct gctgagctaa gtcggcgctg gcccaccacc gaggactcga
      361 acctcgcacc gtcaacttag aaggttgatg ctctatccgg atgagctagt ggtggttggt
      421 ggcccttgct ggacttgaac cagcgacctg gcgattatga gtcgctcgct ctgaccaact
      481 gagctaaagg gccggaggca gaataataac catatgtcat cacatctgca aactcatctg
      541 accaccagca cgtttaacgt cctgtgccgt ttttcaggta taaaaaaacc cgctcatcgg
      601 cgggtttaag ctgtgtggcg tagtaaccac tcttaacagg atattcaact ttttacgatc
      661 gtaaagtttt cggaaaaaat ttttaaaacc gcatcaaccg ctccaccagt tgctctttac
      721 gggcaacgat ccacccgtgt tgctccagat aaaatttaaa ccgttccaga gtacatacca
      781 tcgcatcggc gggtaccttt tccgtgaact caacctgacc gtgtttatcg aagtggctca
      841 gtaatgcgca tccatcattt tcgatagatg tgttttgtgc cgctggtggc tgtttctggt
      901 tgaaataaca gtcttccagt ttttcgaaca cttcccacgc ctgatcggtt tcgagcattt
      961 tggcgtgacg ggctgcgcca cgttctgtcc agaggatgag ggagcgaacg ttacgggcaa
     1021 ttttcacaga aactcttaaa gagttttcgt tcttcaactc tcgtaacgca tcaccttcta
     1081 tcaggaaaaa atgtttacct tctataaacc gttctttgtt tcgattgaaa ttgtttgtca
     1141 aacgctggcg ctctgttccg tacaaatcag ccagcagctc tgttgtcatg acaggaagat
     1201 ggttatgcgt aagtgacggg agtttttcaa tagacgtgcg attcatagac atgtcctttc
     1261 gaatattttg atttacccct tttgagaggg tgaccgggcg ctcaaaaccg tcgaaagtcg
     1321 gcgggcgtat ttccattgct ggtattgtat tagccgcaca cccggtcata aaccaagaat
     1381 tctggacaca aaaaaaccac atggctatcg ggtgtggata ccgctttcga ggtgttttga
     1441 gcaccgcggg gaaatgtaaa ctgcaaacct gaaactgtca acaccgtcag gttccaatca
     1501 tcgggtggtg agacgcacag ggttggaact accgggaaac cgaccggcga gcttttcagc
     1561 tcccccatgc gccccaccat aattcagatg tgcgcgtgca tacgacaata aaaaacacgc
     1621 tcgcggcgtg tgtctgtcgc ggtctctatc cggggttcca atcccgacgc cagattttgc
     1681 tggcgcgtga ggaatatagc cccggacaat gtgtctggtc aagcacctac ataattcgtt
     1741 ctacgtatct gtccatctcc agccggatat caagcatcat caacatgcca tcaataaccc
     1801 cttccgcttt ctgcaggcgc ttgccaatac aggtatccga acacccatgt ttccgtgcca
     1861 gactcataaa agtcattcca cctacgtaat aatccaccag caaatcgtgc aaatcctgat
     1921 ttttcttgtt caaccgggcc atacagccac aaattatcat tgcatcatca tcagaacact
     1981 gagggcgtga tttcactttc gacggaatta accctttaaa accagcagcg atcgacgccc
     2041 aggatacatc ttcatgagtg tttgcagccc aagcccccca ccgttccata acctgctgaa
     2101 tatcacgcgc catcgttatc acctgtgatt tcataaatct tcacgcccag tcgcccacca
     2161 ggaacgagct gaccgcgcac aatattgatt tcatcaaact gctcgtcgtc tatgagtagt
     2221 ccggcatgcg tcag
//
LOCUS       DAJVYM010000275         2191 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVYM010000275 DAJVYM010000000
VERSION     DAJVYM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.280"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..74)
                     /locus_tag="OQS60_003984"
     CDS             complement(<1..74)
                     /locus_tag="OQS60_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCS2389597.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTS"
     gene            complement(71..451)
                     /locus_tag="OQS60_003985"
     CDS             complement(71..451)
                     /locus_tag="OQS60_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS2389598.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(452..1438)
                     /locus_tag="OQS60_003986"
     CDS             complement(452..1438)
                     /locus_tag="OQS60_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS2389599.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(1512..1784)
                     /locus_tag="OQS60_003987"
     CDS             complement(1512..1784)
                     /locus_tag="OQS60_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389600.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            complement(2088..>2191)
                     /gene="hokD"
                     /locus_tag="OQS60_003988"
     CDS             complement(2088..>2191)
                     /gene="hokD"
                     /locus_tag="OQS60_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS2389601.1"
                     /translation="IVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          503 a          593 c          536 g          559 t
ORIGIN      
        1 gacgttgagg ccgatgcatc agtcaggccg ggggattttg gggagtagta tttcggtaaa
       61 tcttccagtt tcattttttg acctgccctt caagcattat gggataaatc ttcaccccca
      121 gacgtccacc agatactggc tgagcacgaa cgatattgat ttcatcaaac tgctcatcgt
      181 ccattagcaa ccccgcatgc gtcagcgcat ccagcggcgc tttcagaata ttgtccaggt
      241 cacggcggcg cttatccggt ggttctgcaa taatttttat tgccaacctt ccggacaggc
      301 ttaatttcag ccgctgctgg cgaacaataa gcgccactgc ccggcgataa cgctccccgg
      361 cttttgatac aaaatatgtg ctgccacgac gacgccagta agtgttcacc gtcggcgggt
      421 aaggtaaaac aaattctatg cgctcggtca tttatgcttt ccacttcaga acacccgaat
      481 ttctcgcgtg cattaaaaaa cgaatcagca acaacagctg actgccgtgt ttctcttcaa
      541 aatcttttac tccggcatgt agttcgttac ggcatttacg gcataacgga ataacaaaca
      601 aatcatcagc ctttgttccc atccctccca gtccatgacc aatgatgtga tgcggatcat
      661 ctgcctgatt gccacacgtc atgcatttct gcgtttttac ccagcgcgtg tatacaggca
      721 tctcttcccg ttgtgatttc tggcgctgga gatactgagc cggtgactcc ggatcaacgg
      781 caatgctgac caccgtcttt tcctgtggtg ggttctgttg ctggtggacg tgaggcagta
      841 acgcaatatt ttttgtgcgc tgcttcagta tgctggtggc tgtctgctct cccggtatga
      901 tgtcgctttc actgtacaca gagcgaattt tttccgcacg caaccccagc gaacgacgta
      961 ataccgcttc cggtagcgcg tccgccacct gattgcggac cgcccaccag gataattcag
     1021 ccagagataa ttcacgctcc tgcgtaccgc ttattgcgtg accgatgacg tcaatcatcc
     1081 atgctgacag gttttgatga gcaagttgct cgagtgattc ggatgtctgg tcacgcagct
     1141 ggttgtcgca gtgccagcac aacaccattg cgccggtacc ataacggtga atgacggttt
     1201 cgctgtgatg ataatcgccg tgtggccact ggcaggattt aatatggcgc aacagccagt
     1261 cagacaatgc accagcacca ccagcagcac gaatcacccg tgcgttactg aaaaacggca
     1321 gcaatgtttt gtcttccacc agcggctggc gagcggcagg aacgaccccg gacggcagat
     1381 tacgcatgct ttttggttcc ggctccacca gtattctgcc gttatggaat gctgacattg
     1441 attcacggcc tggcttaacg ataaccagac cgagttccgg taccagaaca ggtcgaagta
     1501 atacccgcac attacctcca gatccgttgc tggaatgtgc gggacggacg cgttggccgt
     1561 tcggagtaag ggagcctgac ggagattatc cagtgacggt agtcgaggct aagggctttt
     1621 ttaacctcgc atccgcgcct gcggtaacac tgaatgagcc attcggcctg ttcttcagtg
     1681 catgggggat gctggtacca atcagattta aatgcgtgag agcgccgccc gtgcctgctg
     1741 gcaaagacgg ctgaattatc agaattgtgt agtctggaag gcagtggctg acaatagcgc
     1801 cattctcaac gcgcacagta tagaggccat cttcactaaa aatttcacgt aattcttcga
     1861 ttttcatcaa cagaatcctt ccagataaat agcactcccc tgttcggggt ccatccctct
     1921 tctccctgcg cgctacttaa gtatttttga ttctattccg gcaccatcta aaaattcaaa
     1981 cgcgttgaaa ataaaaacaa aaacccgccg aagcgggtta agtgtgggtg cattgaggat
     2041 gcctgaccca tcagaggtgg cgagggattt ctcccccgcc tggtctctta ctcctcaggt
     2101 tcgtaagctg tgaagacagc gacctccgtc tggccggttc gaattcgtac ctcgcagagg
     2161 tctttcctcg ttaccagtgc cgtcactatg a
//
LOCUS       DAJVYM010000276         2094 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVYM010000276 DAJVYM010000000
VERSION     DAJVYM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.281"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(132..1151)
                     /locus_tag="OQS60_003989"
     CDS             complement(132..1151)
                     /locus_tag="OQS60_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS2389602.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            1297..2094
                     /locus_tag="OQS60_003990"
                     /pseudo
     CDS             1297..2094
                     /locus_tag="OQS60_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          557 a          476 c          424 g          637 t
ORIGIN      
        1 ttcaagccca aaatactgtt caggtttagt gagtcacggt cttacctgtc tttgtcatca
       61 ctctggccgc catagcaacc tttcagcatt cacccttttg ttaagagttc attgtttaat
      121 ggtatcagcg attaatatgg tctgatactg acgtggttcc tgtcatactt acggtcatgg
      181 gcggttatcg cccatactgt tcgtgccagc ttgtttgcca tggcgacgat tgccacactg
      241 gctggacgac gttttttcag ttcggttatc cacgggccag gctctttagc tactaatgcc
      301 accgctcttg caccgtggat aaataatgtc ctgagataag tgtcaccacg tttgcttatc
      361 cccagcagac gtactttccc tccggagcca gtttgttttg gaaccagacc aacatatgcg
      421 gcgaactctc gccccgattt aaacgctgat gcttccccca tggtggccac cgctgccgtc
      481 gcaataagtg gtccaacgcc aggaatatcc agcaaccgct tacaggtttc attctgcctc
      541 gccacgctgg taagctgttt ttcaatatcc tctatcagtg agtccagctc attcagtcgg
      601 ttgtactggt tttccagaac cataatgaga tacggtggca gtctctcttt catccgttcc
      661 agtgcttcgg ggaactcccg ctccatcgct gcccggcctt tgtggatggt ttcaccaaac
      721 tccagtaacg tcccgtgcag ggcattaatt tgtgcggtcc ggaacttcac cagttgcatg
      781 cgggtacggt gcagaaccag taccgactgc tgttcttctg tttttacggc gatttcttta
      841 cccggctgct gaaccgccat ccagatagcc cgggcatcca tcacatcatt cttattgccc
      901 atgacgaatg ccttaacgaa gcgggcctgc aacaaccgga ctttatgacc aagttttgtc
      961 agttcccgtg cccagtgctg agaacctcca caggcctcca taccaatcag gcatggctca
     1021 cggttgccga agaacgtcag aaaatcctgt ctacgcaact gtttatcaac cacttcacct
     1081 gtgtgctcat tgatgaagtg aatctgaatg acatgttttg cgatatcaac gccaaccggt
     1141 gtatatttca tttgtggagt ctccagtctg agggagcttt atgcatcccg tattgggcac
     1201 tatgatgccg gaaatctgcg aggctccaca cctgccagtt tcaccctttt aaccatcata
     1261 ctgctccgtg tttaattgtg tgggatgcgt tcattacatt ttcgcgaatg tcagattaga
     1321 aattcaaact tcgataattg ttatctttac gcttcgcact tcaccagagc agagtttctg
     1381 tctgccaaag aaatatcatt tattaaatcg aatttgacag ctgttatgtt tgatcatgtg
     1441 cgaatgtcga cagggaattt taaagattgc attacagaac aattggaatt aactattgat
     1501 tattcagata tatttgggaa tgaagatctc gatggttata tcaataacat tataaaaatg
     1561 attgatacat tgccagataa tgcaatgata ttgaaatccg ttctggcagt aaaactggtg
     1621 atgcaattaa aaattctcaa tattgttaat aaaaacttta ttgagaatat gaaaaaaaca
     1681 tttagccatt gtccttatat aaaagatcca attatacgta gttatataca ttctggggaa
     1741 gatgacaagt tcgataattt tatgcgtcaa catcgattca gtaaggtgaa tttcgatacc
     1801 caacagatga tccattttat taacagattt aatatgaata aagggctgat tgataaaaat
     1861 aacaattttt ttatccaact tatcgatcag gccttacgat caacggatga tatgatcaaa
     1921 gcaaatgcct ggtatcttta taaagagtgg attcgtagtg atgatgtttc acctatattt
     1981 atagaaactg aagaaaagtt aagaaccttt aacactaata aattaacacg aaacgataat
     2041 atatttatcc tgttctcctc tgtcgatgat gggcctgtta tggtggtaag ctcc
//
LOCUS       DAJVYM010000277         2089 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVYM010000277 DAJVYM010000000
VERSION     DAJVYM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.282"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(60..1025)
                     /locus_tag="OQS60_003991"
     CDS             complement(60..1025)
                     /locus_tag="OQS60_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCS2389603.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OQS60_003992"
     CDS             complement(1879..>2089)
                     /locus_tag="OQS60_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389604.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          578 a          550 c          435 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAJVYM010000278         2083 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVYM010000278 DAJVYM010000000
VERSION     DAJVYM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.283"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..113)
                     /locus_tag="OQS60_003993"
     CDS             complement(<1..113)
                     /locus_tag="OQS60_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS2389605.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVA"
     gene            374..709
                     /gene="iraM"
                     /locus_tag="OQS60_003994"
     CDS             374..709
                     /gene="iraM"
                     /locus_tag="OQS60_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS2389606.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(990..1121)
                     /locus_tag="OQS60_003995"
     CDS             complement(990..1121)
                     /locus_tag="OQS60_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCS2389607.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1621..1697)
                     /locus_tag="OQS60_003996"
     tRNA            complement(1621..1697)
                     /locus_tag="OQS60_003996"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1661..1663),aa:Arg,seq:tct)
     gene            complement(1798..1873)
                     /locus_tag="OQS60_003997"
     tRNA            complement(1798..1873)
                     /locus_tag="OQS60_003997"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1838..1840),aa:Ile,seq:cat)
     gene            complement(1976..>2083)
                     /locus_tag="OQS60_003998"
     CDS             complement(1976..>2083)
                     /locus_tag="OQS60_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2389608.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          674 a          522 c          366 g          521 t
ORIGIN      
        1 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
       61 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      121 tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      181 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      241 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      301 ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      361 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      421 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
      481 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
      541 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga attcaatcaa
      601 aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
      661 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
      721 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
      781 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
      841 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
      901 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
      961 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1021 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac tgacattatc
     1081 cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga
     1141 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1201 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1261 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1321 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1381 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1441 ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg tataacaaac
     1501 actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt ttcaacaata
     1561 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     1621 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     1681 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gcccccgcac
     1741 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     1801 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     1861 gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca ataaaaaacc
     1921 cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc catagttatg
     1981 acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac tgccgctcag
     2041 cccatgcttc ttcaatatca aaccgagtca ccagcgcatc ata
//
LOCUS       DAJVYM010000279         2042 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVYM010000279 DAJVYM010000000
VERSION     DAJVYM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.284"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            12..521
                     /locus_tag="OQS60_003999"
     CDS             12..521
                     /locus_tag="OQS60_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS2389609.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            518..1936
                     /gene="phrB"
                     /locus_tag="OQS60_004000"
     CDS             518..1936
                     /gene="phrB"
                     /locus_tag="OQS60_004000"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS2389610.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          484 a          503 c          566 g          489 t
ORIGIN      
        1 caggagcagt aatgaatcta caacgatttg atgacagcac cctaatccgt atctttgccc
       61 ttcatgagtt acatcgactg aaagaacatg gcttaacgcg cggggcgctt ctcgattatc
      121 acagccgcta taaactcgtc tttctggcgc attctcagcc ggagtaccgc aaacttggcc
      181 cgttcgtggc tgatattcac cagtggcaaa atctggatga ctattacaac cagtaccgcc
      241 aacgcgtagt tgttttgctt tctcaccccg ccaacccgcg cgatcacacc aatgttttga
      301 tgcacgttca gggttatttt cgcccgcata ttgattccac agaacgccag cagctggctg
      361 cgcttatcga cagttatcgc cgtggcgagc aaccacttct tgcgccgctg atgcgtatca
      421 aacactatat ggcgctttat catgacgcct ggctttcagg gcagcgttat ttcgaacttt
      481 ggccgcgtgt gattaacttg cgccattcag gagttttatg actacccatc tggtctggtt
      541 tcgccaagat ttacgtctgc acgataatct cgcactggct gccgcctgcc gcaattcgtc
      601 tgcacgcgtg ctggcgttgt atatcgctac accacgccag tgggcgacgc ataacatgtc
      661 gccgcgtcag gctgaactta tcaatgctca actgaatggg ctacaaatag cgcttgcgga
      721 aaaaggtatt cctttattgt tccgtgaagt ggatgacttt gtcgccagtg tcgaaatagt
      781 taaacaggtg tgcgcggaaa acagcgttac ccacctgttt tataactatc agtatgaagt
      841 gaatgagcgg gcgcgggatg tggaagttga aagagcgctg cgtaacgtgg tgtgtgaagg
      901 atttgatgac agcgtgatcc tgccgcctgg cgcggtgatg accggtaatc acgagatgta
      961 caaagtcttt acgcctttta agaatgcctg gctgaaacgg ctgcgggaag ggatgccgga
     1021 gtgcgtcgct gcgccaaaag ttcgtagtag cggatcgata gagccctcgc catccattac
     1081 gctgaattat cctcgtcagt ctttcgatac tgcgcatttt ccggtggaag aaaaagcggc
     1141 gattgcgcaa ttacgccagt tttgccagaa cggtgccgga gaatatgagc aacaacgaga
     1201 ttttccggca gtggaaggca ccagccgttt gtcggccagc ctggcaacgg gcgggttatc
     1261 gcctcgccag tgcttgcatc gcttgttggc tgaacagccg caggcgctgg acggtggggt
     1321 cggtagtgtc tggcttaatg agctgatctg gcgcgagttt taccgtcacc tgataacgta
     1381 tcacccctcg ttgtgtaaac atcgtccaat tattgcctgg acggatcgtg tacagtggca
     1441 gagcaatccc gcacatttac aggcctggca ggaaggcaaa acgggatacc cgattgttga
     1501 tgccgctatg cgtcagctta acagcactgg ctggatgcat aacaggctac ggatgattac
     1561 agccagtttt ctggtgaaag atttattgat cgactggcgc gaaggcgagc gatatttcat
     1621 gtcgcagctg attgatggtg atttggcagc caataacggt ggctggcagt gggccgcttc
     1681 aaccggaacc gatgcagcgc cgtattttcg tattttcaac ccgacaaccc agggcgagaa
     1741 atttgatctc gaaggtgagt ttatccgcca gtggctaccg gaactacgca atgtaccggg
     1801 gaaatcggtg catgagccgt ggaagtgggc gcagaaagca ggtgtgaagc tggattatcc
     1861 gcaaccgata gtcgagcaca aagaggcgag agtacaaacg ttggcagcgt atgaggcggc
     1921 gcggaagggg aaataacaga cgcgtcaggc aatcgagccc agatgccgga tgcggcgtga
     1981 acgccttatc cagcctacaa atttgtgcaa attcaataaa ttgcaggaaa tacgtaggcc
     2041 tg
//
LOCUS       DAJVYM010000280         2013 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVYM010000280 DAJVYM010000000
VERSION     DAJVYM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.285"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(37..273)
                     /locus_tag="OQS60_004001"
     CDS             complement(37..273)
                     /locus_tag="OQS60_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS2389611.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="OQS60_004002"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="OQS60_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS2389612.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="OQS60_004003"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="OQS60_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS2389613.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..>2013)
                     /locus_tag="OQS60_004004"
     CDS             complement(1875..>2013)
                     /locus_tag="OQS60_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCS2389614.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          527 a          530 c          439 g          517 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAJVYM010000281         1965 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVYM010000281 DAJVYM010000000
VERSION     DAJVYM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.286"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..444
                     /locus_tag="OQS60_004005"
     CDS             <1..444
                     /locus_tag="OQS60_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS2389615.1"
                     /translation="YADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKA
                     EINEEQLAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSG
                     FYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM"
     gene            621..983
                     /locus_tag="OQS60_004006"
                     /pseudo
     CDS             621..983
                     /locus_tag="OQS60_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(987..1965)
                     /locus_tag="OQS60_004007"
                     /pseudo
     CDS             complement(987..1965)
                     /locus_tag="OQS60_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          551 a          510 c          465 g          439 t
ORIGIN      
        1 tatgcggatc tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg
       61 gcgtttgagg gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg
      121 ttccgtaagg cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa
      181 gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt
      241 gcccgtgaag gcacgccgca tgtgatgtgt gcttacctgt acgatctggc tggtctgttc
      301 tctggcttct acgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc
      361 cgtctgaaac tggcacaact gacagcgaag acgctaaagc tgggtctgga tacgctgggt
      421 attgagacag tagagcgtat gtaatcgatt tttcgtgaga gtaaagcctg atcagggtta
      481 cctgatcagg cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt
      541 tctgactgtt ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt
      601 tgaatttaag gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt
      661 gccaggctta cgcagaagat acggttatag aaaacgacca tgacatttca gccatcaaag
      721 atggttggca aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc
      781 gacaagaata caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt
      841 atatagaaca agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc
      901 ttaaaaaatc acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt
      961 caaatgagga cgttcaggat ttaatgtaca tcgttccacg tcgcttcgac aacggtatta
     1021 ttctctgcca tttttaccgg tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc
     1081 caccaggcca gcccgagcaa tccggcagtg aacagcaaaa ataccaggtt cggcattcca
     1141 ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca acataacgct tgggttactg
     1201 aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg
     1261 acccccgcgg cggtaaaaat caccagcgcc ggaatttgtg ccaccagacc atcgccaatg
     1321 gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg cagcacgccg
     1381 accagcaacc cgccgacaac gttaatgacc atgatgagga tcccggcgat ggcatcgccg
     1441 cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg agtcacttcg
     1501 gagcggcgtt ttttcgcctc atcttcacca atcaatccag cgttaaggtc ggcgtcaatc
     1561 gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc tgcgatacgc
     1621 ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacaaa caccacgata
     1681 ccgatagcga aattgccacc aacgaggaag tgaccgaacg cttcgaccac cttccctgcc
     1741 gccgccgcgc cggtatgccc ttccatcaaa atgatacggg ttgaagccac gttaagtgcc
     1801 agacgcaaca acgtagtaaa caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc
     1861 tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa ggtaaacaac
     1921 aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaag
//
LOCUS       DAJVYM010000282         1919 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVYM010000282 DAJVYM010000000
VERSION     DAJVYM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.287"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..106
                     /locus_tag="OQS60_004008"
     CDS             <1..106
                     /locus_tag="OQS60_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS2389616.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
     gene            103..1494
                     /locus_tag="OQS60_004009"
     CDS             103..1494
                     /locus_tag="OQS60_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS2389617.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            1491..1748
                     /locus_tag="OQS60_004010"
     CDS             1491..1748
                     /locus_tag="OQS60_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389618.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            1748..>1919
                     /locus_tag="OQS60_004011"
     CDS             1748..>1919
                     /locus_tag="OQS60_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389619.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIK"
BASE COUNT          498 a          436 c          560 g          425 t
ORIGIN      
        1 gttcatcagc ccccgcattg ccgacaggat cacagacggg gggcgcaact ggttgtcgtt
       61 caactggccc agctaccgtt cttgtatcag aggtgtggct gcatgacaac accagtctgg
      121 cgtaatgatg acctggaagg cgctgtcatt ggcgcattct ttctgcgtgg ggcagatcat
      181 gaagtgatgg atattctgat cacactaccg gcggacattt tttctgtacg agcgtatcgg
      241 gatatctaca caggcatctg cagacaggcc cgtgtttcag gagtgattga ccccgtgctg
      301 ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg taaaacctgg
      361 gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc actgcgcgat
      421 gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac ctgtgaagca
      481 gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac aggaaagggc
      541 gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg tgttgaatgc
      601 cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga actggacgca
      661 aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc ttcgatgggg
      721 aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca tggtgtgctt
      781 ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc tgcggctgga
      841 gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga ctgggcgcgt
      901 ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga ccaggcgaac
      961 ctgaccattg atgagatatg cgcaacaacg aaacaccatc tgattaaaca tccggaaacg
     1021 gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg gcgtcatgac
     1081 cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg tggttttccg
     1141 ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa acgtcccatg
     1201 aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat tctgatgctt
     1261 tacagggatg aagtgtacaa cccggataca caggctacag gcatagcaga aattaatatc
     1321 acgaaacaac gtaacggttc tctggggacg atttaccggc gtttttataa cggacatttt
     1381 ctgcctgtgg accaggaaag cgcacaggtt ctttccaccc caatgcggca gccccagccg
     1441 cgcagataca gcaatacacg taccgacagc agtaagatgg agcgtttctt ttgaacaacc
     1501 agacaatgac ttttacctct gaacaattgc gtaaacacgc gcaggaaatg ttgcgacagg
     1561 cggaacaact ggaaaaaaca ggtgtaacaa aagatgccat tcgtcgggat atggtgccag
     1621 cgctcaggga actgatgcag gcgaaacatc gcgcacaaaa agcggtggat gagctcgttg
     1681 attgtgtggc agagctggaa accaaagttg gaaagtttga aaaaatggtg caggaggtgc
     1741 tgcgctgatg cgccatgagt ttattttacc ttatccgccg acggtgaata cttactggcg
     1801 acgtcgtggc agcacatatt ttgtatcaaa agttggtgag cgttatcgcc gtgatgtgac
     1861 gctaattgtt cgccagcagc ggctgaaatt aaacctgtcc ggaaggctgg cgataaaga
//
LOCUS       DAJVYM010000283         1870 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVYM010000283 DAJVYM010000000
VERSION     DAJVYM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.288"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OQS60_004012"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OQS60_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCS2389620.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1870
                     /gene="istB"
                     /locus_tag="OQS60_004013"
     CDS             1253..>1870
                     /gene="istB"
                     /locus_tag="OQS60_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCS2389621.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGE"
BASE COUNT          473 a          462 c          533 g          402 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa
//
LOCUS       DAJVYM010000284         1818 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVYM010000284 DAJVYM010000000
VERSION     DAJVYM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.289"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="OQS60_004014"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="OQS60_004014"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS2389622.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="OQS60_004015"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="OQS60_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1818)
                     /locus_tag="OQS60_004016"
     CDS             complement(1450..>1818)
                     /locus_tag="OQS60_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS2389623.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAJVYM010000285         1772 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVYM010000285 DAJVYM010000000
VERSION     DAJVYM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.290"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(423..680)
                     /locus_tag="OQS60_004017"
     CDS             complement(423..680)
                     /locus_tag="OQS60_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389624.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OQS60_004018"
     CDS             complement(677..1174)
                     /locus_tag="OQS60_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS2389625.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..>1772)
                     /locus_tag="OQS60_004019"
     CDS             complement(1376..>1772)
                     /locus_tag="OQS60_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS2389626.1"
                     /translation="AVNHYRDNAIAYKEQRDNKASELEKANATIADMRKRQRDVAELD
                     ARYIKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGMGDGESPGLTADAEL
                     NYYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          393 a          463 c          408 g          508 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag cc
//
LOCUS       DAJVYM010000286         1729 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVYM010000286 DAJVYM010000000
VERSION     DAJVYM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.291"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(24..1082)
                     /locus_tag="OQS60_004020"
     CDS             complement(24..1082)
                     /locus_tag="OQS60_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCS2389627.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1190..1430)
                     /locus_tag="OQS60_004021"
                     /pseudo
     CDS             complement(1190..1430)
                     /locus_tag="OQS60_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1655..>1729)
                     /locus_tag="OQS60_004022"
     CDS             complement(1655..>1729)
                     /locus_tag="OQS60_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2389628.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          412 a          507 c          379 g          431 t
ORIGIN      
        1 gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg tacttcccga
       61 accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg cccgagcgcc
      121 agtgccgctt tcactgtcga acccaacccc atgaaaaaat ctgcaatcag gtcacccgga
      181 cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca cggatgtttc
      241 ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac ctgcaccgtc
      301 acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata ctgccggttc
      361 agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc acgccgatgc
      421 ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct ttcattcggc
      481 aactgccact gaccggcact gaaccagtgc gacaccatgt ttttctttcc tgtggcatct
      541 gcaatctgtt ttgccgttat ccccagggca gctcgcgcat cacgaaagta agaaatcagc
      601 ggggccatca catgctgttt cagtgcactg cccttcgccg catacccggc atctttcgga
      661 cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc
      721 cgcaggcttt ccttgttgca tccgttccag cgtccggacg gcttcgccca gataatatgg
      781 ttcagcacac tgaagcgttc acgcatcatg atttcgatat cagatgccag gcgatgacca
      841 cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc cagacactgg
      901 tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc ctcaggcttc
      961 actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg taacgaccgg
     1021 ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac agtattaagc
     1081 atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag cagtgggcct
     1141 gaggtttgct tgtgaaccca acgcatgagc agatggctgg tgggtgcccc taacacccac
     1201 cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg
     1261 aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg gcagcgttcg
     1321 cgtgaaaggt aagtattctg cgctatctcc ccgactgtcg ccggttcggt aacgcttaat
     1381 tcattaaaca ccactctggc ggtttctgtc atatcctgct gttttagcat gtcttttcct
     1441 tttccggtta acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagaca
     1501 ggtattcacc gccaccagcg cgtttactgt actgacgcga tttcagtcat aaaaaacccg
     1561 ctcggcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca tcgttatgac
     1621 gaatttacca cagattccgg aaaaatcaac cttgttacct agttaccttt tttaactgcc
     1681 gctcagccca tgcttcttca atatcaaacc gggtcaccag cgcatcata
//
LOCUS       DAJVYM010000287         1705 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVYM010000287 DAJVYM010000000
VERSION     DAJVYM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.292"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            288..1340
                     /locus_tag="OQS60_004023"
     CDS             288..1340
                     /locus_tag="OQS60_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCS2389629.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          549 a          314 c          369 g          473 t
ORIGIN      
        1 taattagtat ggaaaaggta actcacattg ccgtcgatct ataaacgaac cttatgagac
       61 tacctcgaca gagcgcatct gcaaatgatt ttatggctga ttagcggcaa catgaaaaat
      121 tcgatggaag atgaaagcgc aggagttgct ggtgttgttt aaggattaag cagaggaccc
      181 acaggttaga gatatgaaac ataaaagtcc gcatatgcac aaaatggcac cctgatgagt
      241 ctgatagact gcattgcatt ataaccagta gggaggcgag gttaggtatg tttttagcat
      301 cattgttgag acgtattgca tttagttact acgattataa agcttataat ttcaatattg
      361 aaaaaacaga ctttgttgtc atccatattc ccgatcagat tggcgatgct atggccatct
      421 ttcctgttat tcgggcgctt gaattgcata aaattaagca tcttttaatt gtaacgtcga
      481 caattaattt agaagtcttt aatgcgctta aacttgaaca gactaaatta acattagtca
      541 caatgactat gcaggatcac gcaacattaa aagaaataaa agatttagca aagaacataa
      601 cacagcaata cggtacgccg gatctttgta ttgaggggat gcgtaaaaag aacctgaaaa
      661 cgatgttatt tatcagtcag ttgaaagcaa aaacgaattt tcaggttgtt ggtataacca
      721 tgaattgcta ctcccctttg tgcaagaacg cgtcccgtat ggatcagaaa ctccgggctc
      781 ccgtacctat gacatgggca tttatgatgc gtgaggcggg ttttccagca gtcaggccaa
      841 tatatgaatt gccactaagt gaggatgtac tcgatgaggt gcgcgaggaa atgcgatcgt
      901 taggatctta cattgcgttc aatttagaag gtagctcgca ggaacgtaca ttttcattat
      961 cgattgcaga aaatctaata gcaaaaattc aaagtgaaac agatatacca atagtgatcg
     1021 ttcatggacc caaaggtgaa gataaagcca gggcattagt ggattgttat aataatgtct
     1081 accgtttatc cttaccaccc tcgattaaac gttcagcagc aatcataaaa gatgcttata
     1141 tcgcaataac tcctgacacc tcaatattac atatggcaag tgcctataat acccctgttg
     1201 ttgcaattta tgctgattac aaaacgcgat ggcccgcaat ggccgatgtc tcggagtcag
     1261 tcgtcgttgg gcaaaaaatt gacaatataa gtctggatga attcgcaaag gcattaaaaa
     1321 gtgttttggc gagaatatga gacagtactt atgcatagta atgccgatct gttatgaacg
     1381 gtggtgtcat caatactctg aggctggtca atcatcacag aaaaccagcc tttgaatcaa
     1441 agcagtactc accggatacg atcatagtca catcgaacgc aattctggcg gtgccgcatt
     1501 tttatttatt cattttctgt gagagaaggg caagctctat agagggaatg taaaaaagag
     1561 agctccgaat ggagcactct ttaagaaatt ataacggaaa atcagaggtt tttataacat
     1621 gtcgcataag caaacccaag cttttcaaat acgctttgat cacctgttgc cagggaaact
     1681 acgtattgtt cgcttgagtt tgcgg
//
LOCUS       DAJVYM010000288         1672 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVYM010000288 DAJVYM010000000
VERSION     DAJVYM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.293"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1488
                     /locus_tag="OQS60_004024"
     rRNA            <1..1488
                     /locus_tag="OQS60_004024"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1583..>1672
                     /gene="rrf"
                     /locus_tag="OQS60_004025"
     rRNA            1583..>1672
                     /gene="rrf"
                     /locus_tag="OQS60_004025"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          420 a          378 c          523 g          351 t
ORIGIN      
        1 cgaagggggg acggagaagg ctatgttggc cgggcgacgg ttgtcccggt ttaagcgtgt
       61 aggctggttt tccaggcaaa tccggaaaat caaggctgag gcgtgatgac gaggcactac
      121 ggtgctgaag caacaaatgc cctgcttcca ggaaaagcct ctaagcatca ggtaacatca
      181 aatcgtaccc caaaccgaca caggtggtca ggtagagaat accaaggcgc ttgagagaac
      241 tcgggtgaag gaactaggca aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt
      301 aggtgaagtc ccttgcggat ggagctgaaa tcagtcgaag ataccagctg gctgcaactg
      361 tttattaaaa acacagcact gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc
      421 ccggtgccgg aaggttaatt gatggggtca gcgcaagcga agctcttgat cgaagccccg
      481 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
      541 ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac tcagtgaaat
      601 tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc gtgaaccttt
      661 actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga ggctttgaag
      721 tgtggacgcc agtctgcatg gagccggcct tgaaatacca ccctttaatg tttgatgttc
      781 taacgtggac ccgtgatccg ggttgcggac agtgtctggt gggtagtttg actggggcgg
      841 tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc ggacatcagg
      901 aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga gcaggtgcga
      961 aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa cggataaaag
     1021 gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc ggtgtttggc
     1081 acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt atggctgttc
     1141 gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc ggtccctatc
     1201 tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac cggagtggac
     1261 gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa atgcggaaga
     1321 gataagtgct gaaagcatct aagcacgaaa cttgccccga gatgagttct ccctgaccct
     1381 ttaagggtcc tgaaggaacg ttgaagacga cgacgttgat aggccgggtg tgtaagcgca
     1441 gcgatgcgtt gagctaaccg gtactaatga accgtgaggc ttaaccttac aacgccgaag
     1501 atgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     1561 cggtctgata aaacagaatt tgcctggcgg ccgtagcgcg gtggtcccac ctgaccccat
     1621 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cc
//
LOCUS       DAJVYM010000289         1668 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVYM010000289 DAJVYM010000000
VERSION     DAJVYM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.294"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(95..409)
                     /locus_tag="OQS60_004026"
     CDS             complement(95..409)
                     /locus_tag="OQS60_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCS2389630.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(414..629)
                     /locus_tag="OQS60_004027"
     CDS             complement(414..629)
                     /locus_tag="OQS60_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS2389631.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(780..>1668)
                     /locus_tag="OQS60_004028"
     CDS             complement(780..>1668)
                     /locus_tag="OQS60_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HCS2389632.1"
                     /translation="NTPTNNPSEDPDIAGSGYYGSASRTNKNWVSSNRPTHFSSWARR
                     GIIPDRMATAILNVAGRTLAFISGKAPEIKPSPGGDTPSGPSEDASIRTISLLPTAGD
                     AAAQGWSIKNGGVQLSDGVFKITKQSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGA
                     LTNNQFGLGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLG
                     EFGNYSNDWLTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLNALTLTDITKNA
                     AYGVEIESLVLEINAPASS"
BASE COUNT          393 a          464 c          388 g          423 t
ORIGIN      
        1 aacagcagct gacaggccgt atttgatttt tgcgctcatg gatatttatc aggatgctac
       61 caatgaaaga tactggaaag ccaactgcaa aaagctaaca acccgtaatc gagttatcag
      121 aactgttaat ttttatggta taccgcgcct ctgaacaggg gcgcgtttct ggcaacagct
      181 cgtccccttc acataacccg gcagcaacat ccaggaagac ctgtctgatg ctccttctgg
      241 ctgctgcctc ataaaactcc agcgcggcac cttcaacacg gtccagcgag atgtccaggt
      301 caaaaatttc accgtcaaag cgttttttgt cccgtaacgc taaagttacc gtaactttat
      361 tctcaaaatt gcggatccct ttcacaatca gttcatagtt ttgagtcatt gaattactct
      421 ccccgtgcag ccttacgacg gtcctctctg attttgaaat acaggttagt cagatatgtc
      481 agcagcccaa acagcagact ccccagcacg cctattgccg cccactgaga cggggaaacc
      541 ctgtccagca actgcaggaa ccagtagccc gttcccaccg ctgacgtggt gtatgacaca
      601 cctgttgtga ttttttccat ctggtacata ccccgtctcc cgttatccgg aagctgacaa
      661 caataaaaaa agccaccagt taagtactga tggctctgat aactcatgca ggcatctcag
      721 acgacccact gacactaccg gtgagtttaa cgataccttc catttggctg gctcactttt
      781 tatgatgatg ccggtgcatt tatctccagc accagacttt ctatctcaac gccatacgct
      841 gcatttttgg taatatccgt cagcgtcagc gcattcagcc ccagtgtcag actgtctttt
      901 atgacctgga atgccgggcc agccactcca ttcagtttcg gagtaaccgt ggcactgccg
      961 gcggtgaaca ccagctccag cgtcagccag tcgttactgt aattcccgaa ctcgcccaac
     1021 tttgtgtttc ctgctttctt gtgatgcatc agattcagtt tgccgtctgt ggtctgggtg
     1081 aagaacgaca tcaggaacgg gttaccagtc ccggtcatcg ccacgacgtc aggtaacgct
     1141 acatcggtat acagataaat tcccagaccg aactggttgt tggtcagtgc gcctgacagt
     1201 cgaaacttac agctcagtct gccaccccgt gtcagcaggg agactgcgtc atccaccggg
     1261 cgcgtcaggg accaggcttt attgctctgc ttggtgatct taaatacacc atctgacaac
     1321 tgaactccgc cattcttaat gctccagccc tgcgcagcag cgtctccggc tgtcggcaac
     1381 agggagattg tgcgtatgga tgcatcttca gacggccccg atggcgtgtc gccgccgggc
     1441 gagggtttga tttccggtgc cttaccacta atgaaggcta aggtgcgacc ggctacgttc
     1501 agaatagcag ttgccatacg atcgggaata atgccacgac gcgcccatga gctgaaatgc
     1561 gtcgggcgat ttgatgatac ccagtttttg ttcgttcggg atgccgaacc gtaataacca
     1621 gacccggcaa tatcaggatc ttctgacggg ttgtttgtcg gtgtatta
//
LOCUS       DAJVYM010000290         1636 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVYM010000290 DAJVYM010000000
VERSION     DAJVYM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.295"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(165..878)
                     /gene="bdcA"
                     /locus_tag="OQS60_004029"
     CDS             complement(165..878)
                     /gene="bdcA"
                     /locus_tag="OQS60_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2389633.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            949..1542
                     /locus_tag="OQS60_004030"
     CDS             949..1542
                     /locus_tag="OQS60_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS2389634.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
BASE COUNT          411 a          410 c          416 g          399 t
ORIGIN      
        1 ggcctgcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat
       61 gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac
      121 ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
      181 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
      241 accataccag cgacctcttc cggttgccca tgtcttttga tagccataaa actatgcaac
      301 atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg
      361 acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gctcgcgcgc catgccttgc
      421 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
      481 acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc ttcaacagag
      541 gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcattcaat
      601 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
      661 ctcttacgaa cgacatcaat gacagcgtct ctgtcagcac tatctgtgaa tactgctgtc
      721 gctccagtct tttgtgccag gtgttcagcg gcatctttcg accccgcata ggtgaatcgt
      781 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgatacc acgactgcca
      841 ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg
      901 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
      961 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
     1021 gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat
     1081 tatcttggta ttaacccccc gagcctctac gcggcttttg gcagtaaggc tgggttattt
     1141 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
     1201 gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgcagatat
     1261 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca
     1321 caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacggccat ttatgactat
     1381 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
     1441 tcagggcttt ctgcgaaggc tcgggagggg cactccatcg aacaactctg tgcaacagct
     1501 gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa
     1561 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgca ttaaggctag
     1621 cggaattgat catctt
//
LOCUS       DAJVYM010000291         1579 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVYM010000291 DAJVYM010000000
VERSION     DAJVYM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.296"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..165
                     /locus_tag="OQS60_004031"
     CDS             <1..165
                     /locus_tag="OQS60_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2389635.1"
                     /translation="SEYIAPLQDAVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPD
                     IEWPANPVRE"
     gene            280..450
                     /locus_tag="OQS60_004032"
     CDS             280..450
                     /locus_tag="OQS60_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389636.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(577..1134)
                     /locus_tag="OQS60_004033"
     CDS             complement(577..1134)
                     /locus_tag="OQS60_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS2389637.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1131..1406)
                     /locus_tag="OQS60_004034"
     CDS             complement(1131..1406)
                     /locus_tag="OQS60_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS2389638.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
BASE COUNT          441 a          328 c          388 g          422 t
ORIGIN      
        1 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
       61 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      121 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      181 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      241 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      301 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      361 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      421 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      481 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      541 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      601 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      661 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      721 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      781 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      841 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      901 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      961 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1021 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1081 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1141 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1201 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1261 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1321 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1381 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1441 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1501 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1561 tcagatacaa tgtcttatt
//
LOCUS       DAJVYM010000292         1544 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVYM010000292 DAJVYM010000000
VERSION     DAJVYM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.297"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..301)
                     /gene="maoP"
                     /locus_tag="OQS60_004035"
     CDS             complement(<1..301)
                     /gene="maoP"
                     /locus_tag="OQS60_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS2389639.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQ"
     gene            420..1259
                     /gene="hdfR"
                     /locus_tag="OQS60_004036"
     CDS             420..1259
                     /gene="hdfR"
                     /locus_tag="OQS60_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS2389640.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1355..1430)
                     /locus_tag="OQS60_004037"
     tRNA            complement(1355..1430)
                     /locus_tag="OQS60_004037"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1395..1397),aa:Trp,seq:cca)
     gene            complement(1439..1515)
                     /locus_tag="OQS60_004038"
     tRNA            complement(1439..1515)
                     /locus_tag="OQS60_004038"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1479..1481),aa:Asp,seq:gtc)
BASE COUNT          403 a          374 c          376 g          391 t
ORIGIN      
        1 cctgcggttt accgccggaa agggtgtgaa aacgttttgg acgcttaata cgcgtcatat
       61 acttggacca cacgcgttct gcttctgtca ctggctcacg ttcgccacgg catactgcta
      121 cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg
      181 cataaccatg acgctcaagc agttgtgcct ctttgatggt gaaatcacca tgacgagaga
      241 atccacgtgg ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca
      301 tcctgcacgc tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt
      361 gacagagtgt aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg
      421 tggatacgga attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag
      481 cggctgaatc gctctatctg acccagtcag cagtgagctt tcgaatcagg caactggaaa
      541 atcaactggg tgtgaacctt ttcacccgcc acagaaacaa tatccgttta accgctgccg
      601 gtgaaaaact actgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgcaagg
      661 aggtggcgca tacctcacga cataacgagt tttctatcgg tgccagcgcc tcgttgtggg
      721 aatgtatgct taatcagtgg ttgggacgct tgtatcaaaa tcaggatgcc catacaggct
      781 tacagttcga agcgcgaatt gcccaacggc agtctctggt aaaacagctg catgaacgcc
      841 agcttgatct tcttattacc actgaagcgc ccaaaatgga cgaatttagt agtcagttgc
      901 tggggtattt cactttagcg ctttatacca gtgccccttc taaactaaag ggagatctta
      961 actatctgcg actggagtgg gggccagatt ttcaacagca tgaggcaggt ttgatcggtg
     1021 ctgacgaagt tcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga
     1081 tgcttaatgg ttgcacctgg ctacccgtta gctgggcgcg taaaaaaggc ggcctgcata
     1141 ccgttgtcga tagcacaaca ctttcacggc cgctttatgc catatggctg caaaatagcg
     1201 ataaaaatgc gttgattcgc gatcttttga aaattaacgt gctggatgaa gtgtattaat
     1261 ctgaatggct ggcaaggatg ccggtagaag gatttacttc ggagaggatt atttcagata
     1321 aaaaaaatcc ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc
     1381 ccaacacccg gttttggaga ccggtgctct accaattgaa ctacgcccct aattagggtg
     1441 gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga
     1501 ctgaactacc gctccaccga attcttttac aaccaccggt ttta
//
LOCUS       DAJVYM010000293         1537 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVYM010000293 DAJVYM010000000
VERSION     DAJVYM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.298"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..960
                     /locus_tag="OQS60_004039"
     CDS             <1..960
                     /locus_tag="OQS60_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2389641.1"
                     /translation="VTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQ
                     LEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLR
                     LPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLK
                     FHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIYW
                     NSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEAR
                     VITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            972..1421
                     /locus_tag="OQS60_004040"
     CDS             972..1421
                     /locus_tag="OQS60_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389642.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          472 a          239 c          424 g          402 t
ORIGIN      
        1 gtgacgctac cggcggagct gtcggtgatg ctggggaggc tggagcggga actgcgcgcg
       61 ggagcggtga gtgcggagag tgaggcatgg cttgcgcagt gcgggctgac ggcggagcag
      121 atggccgcgc agctggaggc ggaatacatc ccggagagga aacttcatct ttaccactgc
      181 gaccaccggg gactgccgct ggcgctcatc agcccggaag gggaaacggc gtggcagggg
      241 gagtatgacg aatggggaaa cctgctgggc gaaaccagcg cgcagcacct ccagcagtca
      301 ctccgtctgc cggggcagca gtatgatgag gagtcggggc tgtactacaa ccgcaaccgg
      361 tactatgacc cgttgcaggg aaggtatatc acccaggatc cgatagggct gagaggtgaa
      421 tggaatctgt ataaatatcc attaaaccct gttcggttta tagattcttt gggtttaaaa
      481 ttccatgtta acggtgatcc aagtgatttt aatcaggctg ttgagtattt aaaacaagat
      541 tcacaaatga aagaaactat agatttttta tcaagctctg aagaaactat aaatatagag
      601 tatattgaag gaaccaatgt tagatttaat tcgaataata tgacgatata ttggaatagt
      661 agagcatctt tattttgttc aactgaatta aactcgaaat cacaatcgcc tgcattaggt
      721 ttgggacatg agtttactca tgcgcagtat tgccttcttg ataaggaaaa tttcatggct
      781 cttttaagcc gaacagataa gaaatatgag aataaagaag aggcaagggt tattacaata
      841 attgaatcgc gtgcggcgaa aactttaggt gagtgtacca gaggagctca tagtggactg
      901 cctttttatc gggtggatgg accgttgcag acgatgaaaa taacgggtac gccagaatag
      961 tgaggttttt tatgatgaaa tatttgatgg ttttattgtc tctattttct ggttcggtat
     1021 tggggatggg gcgagtgaat gaattgtgtg gaatagatag cgtgaaaacg atagagataa
     1081 ttaatcttcc atcgtatgtt accacgttag ttccgcttag caaggaaggt cttaatgaaa
     1141 tatacagata taaagtggtg gtgaatgaaa ttagagatct ttatgctggt aagataattg
     1201 atttattgca aatgaagtat ttcagaaaag aaaaatataa caatataaga tgggggggta
     1261 gtatcatttc gaaaggtaat aataaatgtg aaatttattt tgatgctttt ggtgaatgtg
     1321 gaagtgtgaa tggcattaac gtgtgttttg aaaaaaatga aatgataggt tggattaaaa
     1381 aagaaatccc tttgctgtcg caaaaaatag gtgggcttta aatggtaggg caatattcaa
     1441 agacagtata ggtaactatt tgtatggcca tgtaaggtaa aagaaaagga ataaaccatg
     1501 agcggaaaac cggcggcgcg gcagggcgac atgaccc
//
LOCUS       DAJVYM010000294         1497 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVYM010000294 DAJVYM010000000
VERSION     DAJVYM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.299"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..1481
                     /locus_tag="OQS60_004041"
     CDS             <1..1481
                     /locus_tag="OQS60_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS2389643.1"
                     /translation="ALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSRRIA
                     QMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPN
                     CGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGP
                     GLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYV
                     LTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIH
                     PQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEAL
                     KRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA
                     YALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLI
                     GTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          335 a          422 c          468 g          272 t
ORIGIN      
        1 aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa atagaccatc
       61 tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc gaaaaagtct
      121 cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag agcgatacgc
      181 tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc cgcacccgta
      241 agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg gcgccgtgct
      301 gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa cagctggagt
      361 tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc tgtactcagt
      421 gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt atcgccggac
      481 cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg ctgtatcgcc
      541 agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg tcgggctggg
      601 tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc tatgtgctga
      661 ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca ggcaataaga
      721 aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc gggtcaacgc
      781 tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat ccgcagaccc
      841 atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc aacgagctgt
      901 accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt aaaatccacg
      961 atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg atcggcgaac
     1021 tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt gccgaacgtc
     1081 agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa aagatgaaaa
     1141 ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata cgccctgaac cagtggccgg
     1201 cgctgacgta ctatgcagat gatggctggg ctgaggcgga caataacatc gctgaaaatg
     1261 cgttgcggat ggtcagtctg ggccgcaaaa actacctgtt cttcggttcg gatcatggag
     1321 gagagcgggg agcgctgctg tacagcctga tcgggacgtg caaactgaac ggagtggagc
     1381 cagaaagcta cctccgctat gtccttgacg tcatagccga ctggccgata aaccgggtcg
     1441 gcgaactgct cccctggcgc gtagcactgc cgactgaata acacattccc gtcaata
//
LOCUS       DAJVYM010000295         1476 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVYM010000295 DAJVYM010000000
VERSION     DAJVYM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.300"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>1476
                     /gene="fimD"
                     /locus_tag="OQS60_004042"
     CDS             <1..>1476
                     /gene="fimD"
                     /locus_tag="OQS60_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
                     /protein_id="HCS2389644.1"
                     /translation="TAGDFRSGNSQQSSPFFFQGTALGGLPQEFTAYGGTQLSANYTA
                     FLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMYTFGTNFQLMGYR
                     YSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSRKDRLQLNISQSL
                     NDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSLSFSWNESVGIPDNEHIVG
                     LNVSVPFNVLTKRRYTRENALDRAYASLNANRNSNGQNSWLAGVGGTLLEGHNLSYHV
                     SQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNWQLSGGVVGHENGITLSQP
                     LGDTNVLIKALGAGGVRIENQTGILTDWRGYAVMPYATVYRYNRIALDTNTMGNSIDV
                     EKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGSLVRENSTGITSMVGDD
                     GQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVT"
BASE COUNT          361 a          346 c          397 g          372 t
ORIGIN      
        1 acggcgggcg attttcgtag cggtaatagt cagcaatcat cacctttctt ttttcagggc
       61 acggcactcg gtggtttacc acaggaattt actgcctacg gcgggacgca attatctgcc
      121 aattacaccg cctttttatt agggctgggg cgcaacctcg ggaactgggg cgcagtgtcg
      181 ctggatgtga cccatgcgcg cagccagtta gccgacgaca gtcgtcatga gggggattcc
      241 attcgcttcc tctatgcgaa atcgatgtac accttcggca ccaattttca gttaatgggt
      301 taccgctatt cgacacaagg tttttatacc cttgatgatg ttgcgtatcg tcgaatggag
      361 gggtacgaat atgattacga ttacgacggt gagcatcgcg atgaaccgat aatcgtgaat
      421 taccacaatt tacgctttag ccgcaaagac cgtttgcagt taaatatttc acaatcactt
      481 aatgactttg gctcgcttta tatctctggt acccatcaaa aatactggaa tacttcggat
      541 tcagatacct ggtatcaggt ggggtatacc agcagctggg ttggcatcag ttattcactc
      601 tcattttcgt ggaatgaatc tgtagggatc cccgataacg aacatattgt cggacttaat
      661 gtttcagtgc ctttcaatgt tttgaccaaa cgtcgctaca cccgggaaaa tgcgctcgac
      721 cgcgcttatg cctccttgaa cgctaaccgt aacagcaacg ggcaaaatag ctggctggca
      781 ggcgttggtg ggaccttact ggaaggccac aacctgagtt atcacgtaag ccagggcgat
      841 acctcgaata atgggtacac gggcagcgcc acggcaaact ggcaggccac ttacggtacg
      901 ctgggggtcg ggtataacta cgaccgcgat caacatgacg ttaactggca gctgtctggc
      961 ggtgtggtcg ggcatgaaaa tggcataaca ctgagccagc ctttagggga taccaatgtt
     1021 ttgattaaag cgcttggcgc aggcggtgta cgcattgaaa atcaaactgg cattttaacc
     1081 gactggcgcg gctatgcggt gatgccgtat gccacggttt atcggtataa ccgtatcgcg
     1141 cttgatacca atacgatggg gaattccatc gatgttgaaa aaaatattag cagcgttgtg
     1201 ccgacgcaag gcgcgttggt tcgtgccaat tttgataccc gcataggcgt gcgggcgctc
     1261 attaccgtta cccagggcgg aaaaccggtg ccgtttggat cactggtacg ggaaaacagt
     1321 accggaataa ccagtatggt gggtgatgac gggcaagttt atttaagtgg tgcgccattg
     1381 tctggtgaat tactggttca gtggggagac ggcgcgaact cacgctgcat tgcgcactat
     1441 gtattgccaa agcaaagctt acagcaagcc gtcact
//
LOCUS       DAJVYM010000296         1430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVYM010000296 DAJVYM010000000
VERSION     DAJVYM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.301"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            13..438
                     /locus_tag="OQS60_004043"
     CDS             13..438
                     /locus_tag="OQS60_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2389645.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1430
                     /locus_tag="OQS60_004044"
     CDS             463..>1430
                     /locus_tag="OQS60_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2389646.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          372 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc
     1141 tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg
     1201 gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat
     1261 atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac
     1321 gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt
     1381 gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga
//
LOCUS       DAJVYM010000297         1422 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVYM010000297 DAJVYM010000000
VERSION     DAJVYM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.302"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..231
                     /gene="hipB"
                     /locus_tag="OQS60_004045"
     CDS             <1..231
                     /gene="hipB"
                     /locus_tag="OQS60_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCS2389647.1"
                     /translation="LANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTT
                     FFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            231..1422
                     /gene="hipA"
                     /locus_tag="OQS60_004046"
                     /pseudo
     CDS             231..1422
                     /gene="hipA"
                     /locus_tag="OQS60_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
BASE COUNT          404 a          316 c          367 g          335 t
ORIGIN      
        1 ttggcgaatg caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg
       61 aaaaaaattg gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc
      121 acgctgacga ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac
      181 gcgaaaaatg cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac
      241 ttgtcacttg gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc
      301 acacctttaa gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt
      361 cgctgccatt gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc
      421 tgttacccga tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca
      481 gacaaccgtt tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa
      541 tacccgaaga cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca
      601 gacttgaaga agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag
      661 aaaatgactt tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag
      721 gcaatgactg gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga
      781 ttggcgaaat caggcagtcc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt
      841 attactgtct gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta
      901 aagcgggaag ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc
      961 gaacggtttt acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat
     1021 cggtgaaata tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg
     1081 ggtccagcga ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt
     1141 tgattggcgc aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca
     1201 gttatcgact cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg
     1261 gaataacatc agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac
     1321 ggcaatcgat aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc
     1381 ggaagtgcag atgcatgaaa tcctaagtga ctttgccaga at
//
LOCUS       DAJVYM010000298         1403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVYM010000298 DAJVYM010000000
VERSION     DAJVYM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.303"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>1403
                     /gene="istA"
                     /locus_tag="OQS60_004047"
     CDS             <1..>1403
                     /gene="istA"
                     /locus_tag="OQS60_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCS2389648.1"
                     /translation="CIGLTLHISASTVFEVLARFKASSLSWPLPADISHDTLEKLIFP
                     PKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSHFCRCYRKWKK
                     TRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTYVEACEG
                     QDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVINDSYQALAEHYGTVI
                     IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARLRELLTDMNNRPMKG
                     YGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYSVPHELVGQ
                     RLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWTPERLIEQG
                     NRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYGPERLEKACYVALHYNAPDR
                     RFIDNLLRHHRDNVELP"
BASE COUNT          344 a          402 c          366 g          291 t
ORIGIN      
        1 gttgtatcgg actgacgtta cacatcagcg cctcaaccgt ttttgaggtt cttgcccgct
       61 tcaaagcctc ttctctgtca tggccattac ctgcggatat atcccatgac acgctggaga
      121 aactcatttt cccgcccaaa gacacctctg cgtcagagct tgttatgcct gacatgctgt
      181 acttcgatac ggaaatgaga aaacctggcg tgacgcgcca actgttgtgg atggaataca
      241 aggcacaggc gggtgacaaa gcgatgggat actcacactt ctgccgttgc taccggaagt
      301 ggaaaaaaac gcgacgcctc tccatgcgac aggagcatcg tgctggcgaa aagctcttta
      361 tcgacttctg tggtcctacg gtccctgtta ttaatccgga caccggagag atacgccgcg
      421 tggcaatctt cgtggctgta atgggggcgt ccaactatac ctatgttgaa gcgtgcgaag
      481 gtcaggacat gatgtcatgg ttaaatgccc acagccgttg cctgaccttc ctgggcggcg
      541 taccgaagct gctcatcccc gataacctca gaagcgcagt gaaaaaagcc gatcgctatg
      601 agcctgtcat caacgacagt tatcaggcgc tggctgagca ctatggcacg gttattatcc
      661 cggcccgccc gcgcaaacct aaagataaac caaaagcaga aaacggcgtc ctcatcgtcg
      721 agcgatggct tcttgcccgc atccgtaatg aaaccttcca cacgctcagg gcgctgaatg
      781 cacgccttcg ggagttgctg acggacatga ataaccgacc aatgaaggga tacggtaatc
      841 aaacccgcgc agaacgcttc cgcatgctgg atgcaccagc actctctccg ctgcctctgg
      901 agccttatga atacacggaa tacaaagccg tgaaggttgg ccctgactac cacgttgaat
      961 atgcccgtca ctggtactcc gttccacacg aactggtcgg tcagcgtctg tcactgaagg
     1021 tggggcagtc ggtggtacag ctctggcaca aggggcagtg tgtggcgcaa catccgcgca
     1081 gcacacatga atataaacac acaaccaatc cgcttcacat gccagaacgc caccggcggc
     1141 atgggacatg gacaccggaa cgccttatcg agcagggaaa ccgtacaggt ccgtccacag
     1201 gccgggtggt tgaatccatg ctgaaagcca aaccccatcc ggagctggct taccgtgcag
     1261 tcctgggtct gctggcactt cagaaaaaat acggcccgga acggctggaa aaggcctgtt
     1321 acgttgccct gcactataac gccccggaca gacgctttat cgacaatctg ctgcgtcatc
     1381 atcgggataa cgttgagctt ccg
//
LOCUS       DAJVYM010000299         1341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVYM010000299 DAJVYM010000000
VERSION     DAJVYM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.304"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            24..506
                     /gene="smpB"
                     /locus_tag="OQS60_004048"
     CDS             24..506
                     /gene="smpB"
                     /locus_tag="OQS60_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS2389649.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            721..1083
                     /gene="ssrA"
                     /locus_tag="OQS60_004049"
     tmRNA           721..1083
                     /gene="ssrA"
                     /locus_tag="OQS60_004049"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          395 a          345 c          308 g          293 t
ORIGIN      
        1 attaccgatg attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga
       61 ccatcgcgct taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg
      121 gacttgccct gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg
      181 acagctacgt ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa
      241 tggccgtggc ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca
      301 accagcgcga actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg
      361 cgctctccct gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta
      421 aaaaacagca cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac
      481 gtatcatgaa aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg
      541 cgcctccctc tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg
      601 ttgccatccc attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg
      661 ataacgccat tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt
      721 ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt
      781 ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt
      841 aataacctgc ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag
      901 gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat
      961 caggctagtt tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact
     1021 aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca
     1081 ccacttttga caggactgca accggacaga gactataaaa acagtcactt acggacactg
     1141 accagacagt aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt
     1201 caaaaaagcc tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt
     1261 taacacgatg cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc
     1321 cagaccactc agctcctctt t
//
LOCUS       DAJVYM010000300         1290 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVYM010000300 DAJVYM010000000
VERSION     DAJVYM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.305"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..209)
                     /locus_tag="OQS60_004050"
     CDS             complement(<1..209)
                     /locus_tag="OQS60_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS2389650.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSK"
     gene            complement(335..913)
                     /gene="flhC"
                     /locus_tag="OQS60_004051"
     CDS             complement(335..913)
                     /gene="flhC"
                     /locus_tag="OQS60_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS2389651.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(916..1266)
                     /gene="flhD"
                     /locus_tag="OQS60_004052"
     CDS             complement(916..1266)
                     /gene="flhD"
                     /locus_tag="OQS60_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS2389652.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT          335 a          321 c          306 g          328 t
ORIGIN      
        1 tatttggagc gacgaaacag caacggcagc gccttcagcg tgcctttaat cgctttgcca
       61 ttattgccga cgataaacga cccaatccct gcaccggcaa taatcaccag ttcagcgggt
      121 tgatagagtg ctccaaggct tccaccggtc atcaaataac cgccgaaaac tgtaccgaga
      181 acaaccaggt aacctaataa gataagcacg acatcatcct tccactgttg accatgacag
      241 gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa aaaagcagcg gtacgtcgtt
      301 accgctactg gaatgttgcg cctcaccgta tcagttaaac agcctgtact ctctgttcat
      361 ccagcagttg tgggataata tcggcaggat tctgggaaag tttacgtctt tttactgccc
      421 gggatggcgg ttgacataag ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa
      481 taaaattgcc gccgcagcag ttgcagctgg aaagttgcag taatccactt tcaacaaacc
      541 gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc ttcttctgct tgtgggcact
      601 gttcaaggta taaacggtag gctttgatca ccgcatcgac gccattacac aaaccggttt
      661 tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg aacgttttgt tcccaggtca
      721 taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt
      781 tataaagttt tatcaggcgt ccgcgactta actgtgtttc gctttccagc atctgcaaac
      841 gagcgcccag ggtaatcaat tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc
      901 ttttttcact catgatcagg cccttttctt gcgcagcgct tcttcaggct gattaacatc
      961 attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg
     1021 ggaatcttgc gtcaactgag taatcgtctg gtggctgtca aaacggaagt gacaaaccag
     1081 ttgattggtt tctgccagct taaccatttg cggaagagtc agtgccgcta acgttgtcgc
     1141 catttcttca tttatgccga gacgaaacat agcggacgct ttgtcctgaa caatcaaacg
     1201 ctgtgcaagt agtaaatatg acaagttgat gtcataaatg tgtttcagca actcggaggt
     1261 atgcattatt cccacccaga ataaccaact
//
LOCUS       DAJVYM010000301         1264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVYM010000301 DAJVYM010000000
VERSION     DAJVYM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.306"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(23..>1264)
                     /locus_tag="OQS60_004053"
     CDS             complement(23..>1264)
                     /locus_tag="OQS60_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2389653.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          334 a          298 c          227 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtca ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAJVYM010000302         1264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVYM010000302 DAJVYM010000000
VERSION     DAJVYM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.307"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            109..>1264
                     /locus_tag="OQS60_004054"
     CDS             join(109..358,358..>1264)
                     /locus_tag="OQS60_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS2389654.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          359 a          310 c          345 g          250 t
ORIGIN      
        1 ccctgccaga ggttcactac gtaacctgac cgacaatgaa gtggcacact gaatttggcc
       61 acctgaacag aggtgatatg ctcacctcag aacaacacag gtgctccaat gaaaaaaaga
      121 aattttagcg cagagtttaa acgcgaatcc gctcaactgg ttgttgacca gaaatacacg
      181 gtggcagatg ccgccaaagc tatggatgtt ggcctttcca caatgacaag atgggtcaaa
      241 caactgcgtg atgagcgtca gggcaaaaca ccaaaagcct ctccgataac accagaacaa
      301 atcgaaatac gtaagctgag gaaaaagcta caacgcattg aaatggagaa tgaaatatta
      361 aaaaaggcta ccgcgctctt gatgtcagac tccctgaaca gttctcgata atcgggaaac
      421 tcagagcgca ttatcctgtg gtcacactct gccatgtgtt cggggttcat cgcagcagct
      481 acagatactg gaaaaaccgt cctgaaaaac cagacggcag acgggctgta ttacgcagtc
      541 aggtacttga gctacatggc atcagccacg gttcggccgg agcaagaagc atcgccacaa
      601 tggcaacccg gagaggctac cagatgggac gctggcttgc tggcaggctc atgaaagagc
      661 tggggctggt cagctgtcag cagccgactc accggtataa acgtggtggt catgaacatg
      721 ttgctatccc taactacctt gaaaggcagt tcgccgtgac cgagccaaat caggtgtggt
      781 gcggtgatgt gacctatatc tggacgggta agcgctgggc gtacctcgcc gttgttctcg
      841 acctgttcgc aagaaaacca gtgggctgtg ccatgtcgtt ctcgccggac agcaggctca
      901 ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg gtgatgttcc
      961 acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg tggcgatacc
     1021 agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca atggaacgct
     1081 tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg
     1141 cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga ccgcacgaat
     1201 ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac tctaactcgg
     1261 tggc
//
LOCUS       DAJVYM010000303         1262 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVYM010000303 DAJVYM010000000
VERSION     DAJVYM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.308"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            304..759
                     /gene="csgB"
                     /locus_tag="OQS60_004055"
     CDS             304..759
                     /gene="csgB"
                     /locus_tag="OQS60_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS2389655.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            800..1252
                     /gene="csgA"
                     /locus_tag="OQS60_004056"
     CDS             800..1252
                     /gene="csgA"
                     /locus_tag="OQS60_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCS2389656.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          382 a          245 c          303 g          332 t
ORIGIN      
        1 aagacatatc gcagcaatca gcgatgggca agaagaatga ctgtctggtg atttttgata
       61 gcggaaacgg agatttaaaa gaaaacaaaa tattttttgc gtagataaca gcgtatttac
      121 gtggctttta atactttggt atgaacaaaa aaagaaaaat acaacgcgcg ggtgagttat
      181 taaaaatatt tccgcagaca tactttccat cgtaacgcag cgttaacaaa atacaggttg
      241 cgttaacaac caagttgaaa tgatttaatt tcttaaatgt acgaccaggt ccagggtgac
      301 aacatgaaaa acaaattgtt atttatgatg ttaacaatac tgggtgcgcc tgggattgca
      361 gccgcagcag gttatgattt agctaattca gaatataact tcgcggtaaa tgaattgagt
      421 aagtcttcat ttaatcaggc agccataatt ggtcaagctg ggactaataa tagtactcag
      481 ttacggcagg gaggctcaaa acttttggcg gttgttgcgc aagaaggtag tagcaaccgg
      541 gcaaagattg accagacagg agattataac cttgcatata ttgatcaggc gggcagtgcc
      601 aacgatgcca gtatttcgca aggtgcttat ggtaatactg cgatgattat ccagaaaggt
      661 tctggtaata aagcaaatat tacacagtat ggtactcaaa aaacggcaat tgtagtgcag
      721 agacagtcgc aaatggctat tcgcgtgaca caacgttaat ttccattcga cttttaaatc
      781 aatccgatgg gggttttaca tgaaactttt aaaagtagca gcaattgcag caatcgtatt
      841 ctccggtagc gctctggcag gtgttgttcc tcagtacggc ggcggtaacc acggtggtgg
      901 cggtaataat agcggcccaa attctgagct gaacatttac cagtacggtg gcggtaactc
      961 tgcacttgtt ctgcaaactg atgcccgtaa ctctgacttg actattaccc agcatggcgg
     1021 cggtaatggt gcagatgttg gtcagggctc ggatgacagc tcaatcgatc tgacccaacg
     1081 tggcttcggt aacagcgcta ctcttgatca gtggaacggc aaaaattctg aaatgacggt
     1141 taaacagttc ggtggtggca acggtgctgc agttgaccag actgcatcta actcctccgt
     1201 caacgtgact caggttggct ttggtaacaa cgcgaccgct catcagtact aatacatcat
     1261 tt
//
LOCUS       DAJVYM010000304         1255 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVYM010000304 DAJVYM010000000
VERSION     DAJVYM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.309"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..580)
                     /locus_tag="OQS60_004057"
     CDS             complement(<1..580)
                     /locus_tag="OQS60_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2389657.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALD"
     gene            673..1056
                     /gene="crcB"
                     /locus_tag="OQS60_004058"
     CDS             673..1056
                     /gene="crcB"
                     /locus_tag="OQS60_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS2389658.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1110..>1255)
                     /gene="cspE"
                     /locus_tag="OQS60_004059"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1110..>1255)
                     /gene="cspE"
                     /locus_tag="OQS60_004059"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS2389659.1"
                     /translation="ADGSKDVFVHFSAIQTNGFKTLAEGQRVEFEITNGAKGPSAANV
                     IAL"
BASE COUNT          303 a          326 c          306 g          320 t
ORIGIN      
        1 tatccagcgc acgagcggca agcaacgttg accagtgatg ctctttgagc ggcccgcgaa
       61 cccaggcggc aggaagtacc aggatttcag ctccctgtaa tgcctgtgcc agcgccagct
      121 ctggaaagcg taagtcataa caggtcatca gaccgacctt catcccctcc acctcgagta
      181 acggagcgat ttcattacca gcatcaacac ggcgtgattc ctgaatggca aatgcatcat
      241 agagatgcaa tttggcataa cgggcgacga tgtttcctgc ctgtagcgcc accagcatat
      301 tccatgcgcg ccccggcgtt gaaggaacat gaatcgtcag aattgtcgtc atcatgttac
      361 gtttactttc tcgccgtaaa cgtccgagga attcgccttc cagcagctgt gctgatttaa
      421 ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc ttccggcagg acgaacagcg
      481 atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca aatctcagcg ttcttttccc
      541 acacagatgt aacagcaaac tgtcctgcgg caaccagcat tgccacctcc tgtcagagtc
      601 gtaaaattcg ccaacgacga ttattcggcg ttacacttat cactcataca aatcaaatag
      661 caggattttg cagtgttaca acttctttta gcagttttta ttggcggtgg tacgggaagc
      721 gtggcgagat ggctgttaag tatgcgattt aacccgctgc atcaggcgat tccgttgggg
      781 acgctggcag caaacctgat tggggcattc atcataggaa tgggattcgc ctggttcagc
      841 aggatgacga acattgatcc agtgtggaaa gtattaatca ccaccggatt ttgtggcggt
      901 ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt tgttacaaga gggccgcttt
      961 ggctgggcgt tactgaacgt tttcgtcaac cttctggggt cttttgccat gaccgcactg
     1021 gctttctggc tgttttcggc ctcaaccgca cactaaagac caaaaaaaac ccgctgatta
     1081 agcgggtttt gaattcttgc tgacgtatct tacagagcga ttacgtttgc agcagaaggg
     1141 cctttggcac cgttagtgat ttcgaactct acgcgctgac cttcagcaag agttttaaaa
     1201 ccattagtct gtattgcaga gaagtgtacg aacacgtctt tgctgccatc agccg
//
LOCUS       DAJVYM010000305         1217 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVYM010000305 DAJVYM010000000
VERSION     DAJVYM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.310"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..93)
                     /locus_tag="OQS60_004060"
     CDS             complement(<1..93)
                     /locus_tag="OQS60_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389660.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            232..903
                     /gene="queE"
                     /locus_tag="OQS60_004061"
     CDS             232..903
                     /gene="queE"
                     /locus_tag="OQS60_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS2389661.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          342 a          236 c          271 g          368 t
ORIGIN      
        1 gttatcatta aaaactaata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg
       61 attaaatcca ttttctttat tttcttctga cataatacgt tactcccttt aattattaac
      121 gatatgtttc gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg
      181 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      241 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      301 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      361 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      421 agtgataagt ggggggcggc gagcagtgaa gacttgttgg ctgtcattag tcgccaggga
      481 tacaccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctacca
      541 ctgactgatc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat
      601 gaggtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      661 ggctatgacg tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg
      721 cgcgtacgcg atattgaagc actggatgaa ctattggcaa cgctgaccga tgataaaccg
      781 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa
      841 acctgcattg cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      901 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta
      961 tattgtttgt cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat
     1021 tctctacaaa ttctgtggca ttaatttttc gctggagtga aaattattgc ggtaaagttt
     1081 ggtagatttt aatttgtata gagttatttt aaatatttac ctttttaatc aatgaattaa
     1141 gtactcttta acataatgga tgtgttgttt gtgtgatact gtaaagttgg tagattgtga
     1201 ctggcttaaa aaatcat
//
LOCUS       DAJVYM010000306         1211 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVYM010000306 DAJVYM010000000
VERSION     DAJVYM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.311"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..680)
                     /locus_tag="OQS60_004062"
     CDS             complement(<1..680)
                     /locus_tag="OQS60_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2389662.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="OQS60_004063"
     CDS             complement(996..>1211)
                     /locus_tag="OQS60_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCS2389663.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAJVYM010000307         1162 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVYM010000307 DAJVYM010000000
VERSION     DAJVYM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.312"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..568)
                     /gene="rutB"
                     /locus_tag="OQS60_004064"
     CDS             complement(<1..568)
                     /gene="rutB"
                     /locus_tag="OQS60_004064"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS2389664.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATH"
     gene            complement(568..>1162)
                     /locus_tag="OQS60_004065"
     CDS             complement(568..>1162)
                     /locus_tag="OQS60_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS2389665.1"
                     /translation="FTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
                     VNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEAL
                     SWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEG
                     VLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          252 a          327 c          323 g          260 t
ORIGIN      
        1 ggtgtgttgc gtcttccagc accacgccga aatactccag aaaaaagcca tcgcgtagcg
       61 tcgattcgac gcagacgttg gtagcgatac cggtgaaaac cagatggcgt attccacggc
      121 tgcgcaaaat gctgtccagc ggcgtattga agaaaccgct gtagcgcggc ttcggcagca
      181 caatatcgcc aggctgcggc accagttcat ccaccagttg ataatcccag gagcctttcg
      241 ccagcaattt cccctgcagc tgcggctgct tacgcatggt tttcagggcg ttcgatttat
      301 gaaaattcgg tgagccgggt ccgccagctt cgacatactg ttcatcccag ccattttgaa
      361 accagatgat cagcatccct gccgctcgcg ctgcggtcac ggcggtttga atgttggcaa
      421 tgaccgggcg agtggttgag acatcaaacc cggcgagatc taagtagccg cctggcgtgg
      481 cataagcgtt ttgcatatcc accacgatca gcgcactttg ctgcggatcg aaggtaatgg
      541 cttccggtcg agcggttaag gtcgtcatca tgccacctcc tgagtcagcg cagggagatg
      601 ggcgcggcac tgcatcagtg gttgaatgcg ctcgccgaag gtttcgattc ccgacagaaa
      661 atcatcgaag gtcaacagca cgccttcggc accaggcacg cttgcgactt catctaacat
      721 gcgcgcgaca ctggcgtaag aaccgactaa tgtccccata ttgatgttta ccgccgaagt
      781 gggatcggcc atctgacgga cgttggtgtc agtaccggag cgggtatctt tctgactttg
      841 ttcggttagc cagcttaacg cctcttcatc cgcgcccgct ttgtagtgtt cccatttggc
      901 gcgagcggca tcgtcggttt catcggcaat caccataaac aacacataag agccaacgtc
      961 gcgcccggtt tgctctgcgg cctgtttcat ccgcacagcg gtcggggcga aagccgtggg
     1021 tgtatttacg cctttgccga aacagaagtt gaaatcggca tactgagcgg agaacgccat
     1081 gccagcgtcg ctttgcccgg cgcagatcac tttcatgggg acactcggtt gcggactgac
     1141 gcgacaatca ttcatggtga aa
//
LOCUS       DAJVYM010000308         1158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVYM010000308 DAJVYM010000000
VERSION     DAJVYM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.313"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(50..193)
                     /locus_tag="OQS60_004066"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OQS60_004066"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..916)
                     /locus_tag="OQS60_004067"
     CDS             complement(197..916)
                     /locus_tag="OQS60_004067"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS2389666.1"
                     /translation="MTIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
BASE COUNT          308 a          244 c          227 g          379 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggtt
//
LOCUS       DAJVYM010000309         1158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVYM010000309 DAJVYM010000000
VERSION     DAJVYM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.314"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            84..830
                     /locus_tag="OQS60_004068"
     CDS             84..830
                     /locus_tag="OQS60_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2389667.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            845..>1158
                     /locus_tag="OQS60_004069"
     CDS             845..>1158
                     /locus_tag="OQS60_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS2389668.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIR"
BASE COUNT          287 a          267 c          358 g          246 t
ORIGIN      
        1 ggcctgagac gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc
       61 caggcttcag ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca
      121 tccgccgact gaccccgcca catgtaaccg ccccattcag aacggtagcg gagtggcgcg
      181 agtggcaact tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt
      241 tgcgggtgga aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt
      301 ttgcgaatta ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca
      361 tcgccgatga actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctggtaccg
      421 ggaagaatca cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga
      481 ttgtggttac cgtggctgat gttatgagcg ccctgcacgc cagctatgac gacgggcagt
      541 caggcgaaaa atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg
      601 gcattcagcg cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga
      661 cagcgtcgat gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat
      721 tgctcggcga gcgggtgatg gatcgcatgg tcatgaacgg cgggcgctgg gtgaatttta
      781 actgggagag ctggcgtccg aatgttagcc attcgagggt tgttaagtag tttcaggagg
      841 atttatggcg aaacctttta ctcccgaaca gcgggaagaa ctgaagacgc gaattgtgga
      901 actcgtgcat caggacggtc gggtcacgat tcggcagttg tcagatgaaa caggtatcag
      961 tcgtgcgtct gtcggtcgct tatgcataga actggtcgca agtggtgatg tatataattc
     1021 tggctacggc ttattcccgt ctgaacaggc tcgcaaggac tggcaaagcg cccgcaaaaa
     1081 actctcgaga gtaaaggtga ggaaaccggt tgttgttgat ccggacctta tctggtcatt
     1141 acctgacgga gaaatacg
//
LOCUS       DAJVYM010000310         1127 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVYM010000310 DAJVYM010000000
VERSION     DAJVYM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.315"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..778
                     /locus_tag="OQS60_004070"
     CDS             <1..778
                     /locus_tag="OQS60_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389669.1"
                     /translation="FSMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALAS
                     ALMFWKILPESRHFRPTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYI
                     GYRLMLSPWHVSQAVVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIA
                     GTLGGVFWHNYGWNGVGAFIALMLVIALLVGTRLHRRLHA"
BASE COUNT          232 a          280 c          280 g          335 t
ORIGIN      
        1 cttttcgatg gggttgtata tcagcggcaa ctcaattggc ggcatgagcg gacgcttaat
       61 tagtggtgtc ttcacggact ttttcaactg gcgaattgct ctggcggcaa tcggttgttt
      121 cgcgctagcc tcggcgttga tgttctggaa aatccttccg gaatcacgcc attttcgccc
      181 gacttcgctg cgccctaaga cgttatttat caactttcgt ctgcactggc gtgaccgggg
      241 attaccgtta ttgttcgcag aaggcttttt gctgatgggg tcgttcgtca cgctgtttaa
      301 ttacatcggc tatcggttga tgctttcccc ctggcatgtc agtcaggccg tggttggctt
      361 attatcgctg gcttatttga ccggtacatg gagctcaccc aaagccggaa ccatgaccac
      421 ccgctacggg cgcggtccgg tgatgttgtt ttcgacgggg gttatgctgt ttggtttact
      481 gatgacctta ttcagctcgc tgtggctgat ctttgccgga atgttactct tctcagcagg
      541 attcttcgca gcccactcag ttgccagcag ctggattggc ccccgcgcaa aacgcgctaa
      601 aggccaggcc tcctcgctgt atctgttcag ttactatctg gggtcgagta ttgccgggac
      661 gctgggtggt gttttctggc ataactatgg ctggaacggc gtcggcgcat ttattgctct
      721 gatgctggtc attgctctgc tggtcgggac gcgtttgcat cgtcgtctgc acgcctgaaa
      781 aataagcccg gactgcggta actacacgtc cggacttatt gcttgctcaa accaacgtta
      841 atagccatcc taaaatagac gaagcgccag ccaattcccg cagcgcgtct agcgtcatca
      901 ggattataag tacccaaata aacggattca ttttgctgtg tgtcatttat tactgatgcg
      961 cagttattct accgctttgt aagtagtaaa atagttaacc cgatcaagac tactattatt
     1021 ggtagctaaa tttcccttaa gtcacaatac gttattatca atgctgtaat ttattcagcg
     1081 tttgtacata tcgttacacg ctgaaaccaa ccactcacgg aagtctg
//
LOCUS       DAJVYM010000311         1124 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVYM010000311 DAJVYM010000000
VERSION     DAJVYM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.316"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..873
                     /locus_tag="OQS60_004071"
     CDS             <1..873
                     /locus_tag="OQS60_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2389670.1"
                     /translation="IPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQM
                     TIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQ
                     AITVTNNQIQNIGVNQIQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQT
                     TVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSA
                     GEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPV
                     PKDLPPGMPDMRQF"
     gene            949..>1124
                     /locus_tag="OQS60_004072"
     CDS             949..>1124
                     /locus_tag="OQS60_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCS2389671.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVL"
BASE COUNT          306 a          248 c          348 g          222 t
ORIGIN      
        1 ataccgcgcg tgggtcagga ggtcattgtg gacttcctca acggcgatcc ggaccagccg
       61 attattatgg ggcgtaccta ccaccaggaa aaccgcacac ccggtagcct gccggggacg
      121 aagacgcaga tgaccattcg ttcgaaaacc tataagggca gcgggtttaa tgaactgaag
      181 tttgacgatg cgacagggaa agaacaggtc tacatccacg tgcagaagaa catgaacacc
      241 gaggtgctga ataaccgcac cacggatgtg ataaacaacc atgccgaaaa aataggtaac
      301 aaccaggcga tcaccgttac caataaccag atccagaaca ttggcgttaa tcagatacag
      361 acggttggtg tcaatctggt ggaaacggtg ggcagtaacc agattatcaa agtgggatca
      421 aaccaggttg aaaaggtggg gatcattcgt gcgctgacgg tgggtgtggc ttaccagacg
      481 acggtaggcg gcattatgaa tacctcggtg gcgttgttgc agtcctcaca ggtagggctg
      541 cataaatcac tgatggtggg aatgggctac agcgtcaatg tagggaataa cgtcaccttc
      601 tcggtgggca agacgatgaa ggaaaacacc ggacaaacag cagtttattc tgccggtgag
      661 catcttgaac tctgctgtgg taaggcaagg ctggtgctga cgaaggacgg aagcatattt
      721 ctcaacggta cgcacattca tctggaaggg gagtcggatg tgaacggtga tgcgccagtg
      781 attaactgga actgtggtgc cacacaacct gtaccggatg cgcctgtgcc gaaagattta
      841 ccccccggaa tgccggatat gcggcaattt tgagtcaggt gcaaaaaatt aaaacggaac
      901 tggtgcagtg accagatgaa ccatattaaa agcagaagga attgattcat gggcggcgga
      961 aaaccggcgg cgcggcaggg cgacatgacc cgcaagggac tggatattgt gcagggttca
     1021 gcgggtgtgc tgataggtgc gccgacggga gtggcctgtt cggtgtgtcc gggagggatt
     1081 acctatgcta acccggtgaa cccggtgctg ggtgcgaagg tgct
//
LOCUS       DAJVYM010000312         1119 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVYM010000312 DAJVYM010000000
VERSION     DAJVYM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.317"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..347)
                     /locus_tag="OQS60_004073"
     CDS             complement(<1..347)
                     /locus_tag="OQS60_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2389672.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKG"
     gene            681..>1119
                     /gene="yhhY"
                     /locus_tag="OQS60_004074"
     CDS             681..>1119
                     /gene="yhhY"
                     /locus_tag="OQS60_004074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2389673.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT          295 a          268 c          289 g          267 t
ORIGIN      
        1 cctttgcttt tcgccagtgc aaacagctct ttcgcctgcg caagtgtcgg agtgaacggt
       61 ttttcgacca gcacattttt cccggcttcc agcgcgcgct tcgcgtactc gaagtggctg
      121 tccgcatggg tgcagacaac aaccaactta acatcgggat cgtttagcac ttcgtcgaga
      181 tcgctggtga aatggatatg agagtaaatg ggagcctgtt cttccggctt cgcatggcga
      241 cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa gtatatacgg cagatggtaa
      301 cgggtggtgc ttttgccgaa gccaataaag gcgcagttga tgaccatgat ttcgtccttt
      361 ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg agtgcgcata
      421 acgaacacaa gcactcccgt ggataaattg agaacgaaag atcaaaaaaa aagccagcac
      481 ccggctggct aagtaatact ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc
      541 gagggtcttc ctgatcgcga gacaataata atcattctca ttcgcacttg tccaacactt
      601 tttgcaaaaa aaatgcattt gactcgcatt tgaaagtcaa tgatgttgaa agggacattt
      661 accccaaaga ggacaaagga atgagtgaga tagtaatacg ccacgcagaa acacgggatt
      721 acgaggccat caggcagatt cacgcccagc cggaggtgta ttgcaacaca ctacaggtgc
      781 ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc atcaagcaac
      841 tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg caacagcacc
      901 cacgccgcag tcatgttgcc gattttggta tctgtgtcga ctctcgttgg aagaaccgcg
      961 gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg ttgcgggtag
     1021 atcgcattga actaaccgtg tttgtcgata acgccccggc aattaaggtc tataaaaaat
     1081 acggctttga aattgaaggg actggtaaga agtacgcat
//
LOCUS       DAJVYM010000313         1117 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVYM010000313 DAJVYM010000000
VERSION     DAJVYM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.318"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            84..1115
                     /locus_tag="OQS60_004075"
     CDS             84..1115
                     /locus_tag="OQS60_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS2389674.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAJVYM010000314         1116 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVYM010000314 DAJVYM010000000
VERSION     DAJVYM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.319"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..934
                     /locus_tag="OQS60_004076"
                     /pseudo
     CDS             1..934
                     /locus_tag="OQS60_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          307 a          216 c          335 g          258 t
ORIGIN      
        1 gtgacgcttc cggcggagct ggcggtgatg ctggggaggc tggagcggga actgcgtgcg
       61 ggtgcggtga gtgcggagag tgaggcatgg cttgcgcagt gcgggctgac ggtggagcag
      121 atggaaagcc agatggaggc ggaatacatc ccggagcgga ggctgcatct ttaccactgt
      181 gaccaccggg gactgccgca ggcgctcatc agcccggaag gggaaacggc gtggcgcggg
      241 gagtatgacg agtgggggaa ccagcttaat gaggagaacc cgcatcacct gtaccagccg
      301 taccgtttgc cggggcagca gtatgatgag gagtcgggac tgtattacaa ccgtcaccgg
      361 tactacgatc cgttgcaggg gcggtatatc acccaggatc cgatagggtt gaagggggga
      421 ataaatctat acacgtatcc actggcacca atacgttaca ccactggggt tagaacgagt
      481 tataagtgta tatgggcctc ccgctccaga ccgggcaggt gctgaaactc ctcttgttct
      541 tactgatatg actggaggag tgactattta ttatgatcct gaaacaggtg attcaatgac
      601 ttttgatagt agcaatagaa ttgatcgtcg ttctcaacgt ggtgctggtg atccatatac
      661 aggtgaagtc gttggttgtg agacaaatgg atcaggaata tcggcagcat atggcacaac
      721 gaagatctat actacagata ctagagcaag atggctacat ggtgggggat catcgttgcg
      781 cgatccttat gctcctcgac aaggctggaa accaactatg ggatgtaccc gtgcacagaa
      841 tgaagatgta gatgagttgt gtaagaaagt gacaagttgg atgtattcgc atcctggaga
      901 gagaattaga tatgaacgtt ttaagacacg ttaatgcgat taacatggga gtgtgcattc
      961 taattttggt tattggatgt gcaaatgcta aagttcttaa acgatgtata ttacccgctc
     1021 ctgatggtgg cgtagaaaaa ctatcaaaac ccaacttaca tggagtaatt aaaaaagttg
     1081 atttgaacac aaatgaagcg gaaattaaaa taaaag
//
LOCUS       DAJVYM010000315         1109 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVYM010000315 DAJVYM010000000
VERSION     DAJVYM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.320"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            759..1055
                     /locus_tag="OQS60_004077"
     CDS             759..1055
                     /locus_tag="OQS60_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS2389675.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          330 a          213 c          236 g          330 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggaagtcct tttaagactg gttatgaagg agctatgagg
      121 ttgattaggg gttggtacaa gcccaactaa ccgaatcaac tatgaagatt ttaggagggt
      181 aactgtaacc atcactttct aactttcacg taggaaattg ttaagtggtt tgatagttac
      241 accatgtgac caagcatcta tatatcgtta tatagccctg tatatagcac tatatgtagt
      301 gtcgagagag gaacgactaa cacgatacag tttaccctga aaagagactt cacagattct
      361 ttatatattt caataagtta gggtaaaaag ggactatacc gagactgtat agaatctgtg
      421 tagaattgca tagatacttg gaaatctact attaaattgc atagggactc acctgatatt
      481 gtcctgtttt taacaggtct cctagaaaac cgaatagact tcattcaagg gtcattgaag
      541 tcataatcat ttcagtagaa aaaccaagtc tcgattttaa atgcaaatag caatcatatt
      601 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
      661 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
      721 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
      781 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
      841 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
      901 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
      961 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
     1021 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     1081 ggtttttgca accgtgttct tgtttggcc
//
LOCUS       DAJVYM010000316         1107 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVYM010000316 DAJVYM010000000
VERSION     DAJVYM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.321"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..322)
                     /locus_tag="OQS60_004078"
     CDS             complement(<1..322)
                     /locus_tag="OQS60_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389676.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGM"
     gene            complement(422..778)
                     /locus_tag="OQS60_004079"
     CDS             complement(422..778)
                     /locus_tag="OQS60_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS2389677.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(914..1021)
                     /gene="rprA"
                     /locus_tag="OQS60_004080"
     ncRNA           complement(914..1021)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQS60_004080"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          346 a          226 c          251 g          284 t
ORIGIN      
        1 ccattcccgc cagaaagcca aaaacataag cgataatgat gttattggtc tgcaggatgc
       61 caaaaaagaa ggccatatag cagcacatcc cgagcatgat aaaagggcgg cgaccaaagc
      121 gatcggataa taatcctgca aaaagcaaga cacttaatcg accaatgccc agcgatgaga
      181 taactatcga gacacccgcg gcattagtct gccaaagtgt ctccagcgag gccatattca
      241 ggctcatcaa aatgacgccc ataccatgca ccaggtaatt aaaatacaac ccaagtgcgg
      301 tagggtaata gggatttttc ataggttcta cttagcaaag gtgctttaaa aagtgtagcg
      361 atgttgtgtc agtatccatt cattattgtt gtaacaacga caattccttt ctgtggaaaa
      421 attaacaaag ccgctccaga tcgtgtgcat ataattcagc ggctaccgat tgatagatct
      481 tccagtcaat aaaattgcca tcagggtaga cttgttgaaa cgcggttaag tcggggaaaa
      541 aacgcatggt gttgttgccg atgcggttat ttattttctc tatgatggca tgaagatgtt
      601 tttttcgttg ctgacgcata gccaacagtt gctcgactac acaatcagga atggcgcatt
      661 tgccattttc ccactgacgc cagctttcac tgtcaccggt ctgggcaatc caggtcgcgc
      721 attcatcaat agtcatggca aaaatatggc ggagtgcctg gagttcataa gcgttcatcg
      781 tgtaatggct gcatgaaata aacaataaaa tataaataac attatcagcc tgctgacggc
      841 ttgaagagag tcacagtatc ttgtgcaaca ttattggaaa gagtgagggg cgaggtagcg
      901 aagcggaaaa atgttaaaaa aaagcccatc gtgggagatg ggcaaagact acacacagca
      961 attcgttgtt tcactcaggg gatttccatg cttataaatc aatatgttga tttataaccg
     1021 tgagctaata gtaggcatac ggactttttg cgtcgatcag attcgtctca ttagtttaaa
     1081 aactgtaaag aatttgcgac aggtact
//
LOCUS       DAJVYM010000317         1106 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVYM010000317 DAJVYM010000000
VERSION     DAJVYM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.322"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            3..983
                     /locus_tag="OQS60_004081"
     CDS             3..983
                     /locus_tag="OQS60_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCS2389678.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1106
                     /locus_tag="OQS60_004082"
     CDS             998..>1106
                     /locus_tag="OQS60_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS2389679.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITA"
BASE COUNT          283 a          255 c          294 g          274 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccg
//
LOCUS       DAJVYM010000318         1097 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVYM010000318 DAJVYM010000000
VERSION     DAJVYM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.323"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>1097
                     /locus_tag="OQS60_004083"
     CDS             <1..>1097
                     /locus_tag="OQS60_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2389680.1"
                     /translation="LTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGWLTE
                     ISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAWNTSGQ
                     LRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIP
                     YATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIR
                     MHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWM
                     SLSRKPEVTWYGWDGDRLTTIQTGTTRIQTV"
BASE COUNT          247 a          318 c          381 g          151 t
ORIGIN      
        1 actcacgcag agtgaagatg aggggcttat caccctctgg cattacgatg cgtcggaccg
       61 tatcacccgg cgcacggtga acggtgaacc ggcggagcag tggcagtatg acgaccacgg
      121 ctggctgaca gaaatcagcc acctgagtga aggccaccgt gtggcggtcc actacggcta
      181 tgacgataaa ggccgtctga cgggcgaacg gcagacggtg gagaccccgg agacggggga
      241 gatgctgtgg gagcatgaga cggggcacgc gtacagcgaa caggggctgg cgacccgtca
      301 ggagccggac ggcctgccgc cggtggagtg gctgacgtat ggcagcggtt atcttgcggg
      361 gatgaagctg ggcggaacac cgctggtcga gtacacgcgg gaccggctgc accgtgagac
      421 ggcccgcagc ttcggcgggg aggcatatga acttgccacc gcctggaata ccagcggcca
      481 gctccggagc aggcacctga accttccgca gcttgaccgt gactacgact ggaacgacaa
      541 cggacagcta atccgcatca gtggcccgca ggagagccgg gagtaccgtt acagcgacac
      601 ggggaggctg acgggcgtcc acaccacggc agcgaacctg gatatcgata tcccgtatgc
      661 aacagacccg gcaggaaatc ggctgccgga cccggaactg cacccggaca gcaccctcac
      721 ggcgtggccg gataaccgca tcgcggaaga tgcgcactat gtctatcgct acgatgaata
      781 cggcaggctg gcggagaaga cggaccgtat cccggaaggg gttatccgga tgcacgacga
      841 gcgcacccac cactatcact acgacagcca gcaccgcctg gtgttctaca cgcggataca
      901 gcacggcgaa ccacaggtgg agagccggta cctctatgac ccgctgggcc gccggacggg
      961 aaaacgggta tggcggcggg agcgtgacct gacggggtgg atgtcgctgt cgcgtaaacc
     1021 ggaggtgaca tggtacgggt gggacggtga caggctgacg acgatacaga ccggcaccac
     1081 gcgtatccag acggtat
//
LOCUS       DAJVYM010000319         1088 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVYM010000319 DAJVYM010000000
VERSION     DAJVYM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.324"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="OQS60_004084"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="OQS60_004084"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS2389681.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAJVYM010000320         1085 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVYM010000320 DAJVYM010000000
VERSION     DAJVYM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.325"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..643)
                     /locus_tag="OQS60_004085"
     CDS             complement(<1..643)
                     /locus_tag="OQS60_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2389682.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="OQS60_004086"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="OQS60_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS2389683.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAJVYM010000321         1070 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVYM010000321 DAJVYM010000000
VERSION     DAJVYM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.326"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..483
                     /gene="yieH"
                     /locus_tag="OQS60_004087"
     CDS             <1..483
                     /gene="yieH"
                     /locus_tag="OQS60_004087"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS2389684.1"
                     /translation="HGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            550..1023
                     /locus_tag="OQS60_004088"
     CDS             550..1023
                     /locus_tag="OQS60_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCS2389685.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT          256 a          239 c          262 g          313 t
ORIGIN      
        1 catggtgtta cgttagcgaa aacagaatct gaacacgttt accgtgcaga agtcgctcgg
       61 ctgttcgatt cagaactgga agccatcgaa ggggctgaag cgctcctgtc agcgatcact
      121 gcgccaatgt gtgtggtatc taacggccca aataacaaaa tgcagcattc tatgggcaag
      181 ctgaatatgt tgcactactt cccggataaa ctgttcagcg gctacgatat tcagcgctgg
      241 aagccagacc cggcgttaat gttccatgcg gcaaaagcga tgaatgtaaa tgtagaaaac
      301 tgcattctgg ttgatgactc agttgccggt gcgcaatctg gtatcgacgc aggtatggaa
      361 gtgttctact tctgcgccga cccgcacaat aagccgatcg ttcacccgaa agtcaccacc
      421 tttacccatc tttcgcagtt accggaactg tggaaagcgc gtggttggga tattacggca
      481 tagttcttca cactcccttc acttaccccg cttaaattgg cgctcaaagg taagtaaagg
      541 gagtttgata tgtctgtttc acgtcgggta atacatcacg gactttattt tgcggtttta
      601 ggaccgttaa ttggtgttct gtttcttgtc ctctacatat tcttcgcaaa agaaccgctg
      661 gttcttttgg taataataca ggtacttcca ttgtttctct tattgtcgat tactacggga
      721 gctattcctg cattgttaac cggcgtgatg gtcgcctgtc tgccggaaaa gatcgggtca
      781 cagaaaaatt atcgttgtct ggctggtggc ataggtggcg tcgttatcac cgagatctat
      841 tgtgcagtta ttgtacatat taagggcatg gcttcctcgg agttgtttga aaacattctt
      901 tctggtgaca gtctcgttgt ccgcatcatt cctgcattgc tggcaggtgt ggtgatgagc
      961 agaatcatta cccgtctgcc cggattggat atttcatgtc ctgaaacaga ctctttaagt
     1021 taagcgggat actttatctt tgggctactc aaaagcagac aggatgttac
//
LOCUS       DAJVYM010000322         1064 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVYM010000322 DAJVYM010000000
VERSION     DAJVYM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.327"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..594
                     /locus_tag="OQS60_004089"
     CDS             <1..594
                     /locus_tag="OQS60_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2389686.1"
                     /translation="RIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWE
                     NNVKSIDALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRA
                     QYPTAPGYATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQ
                     DKRYHIVEGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            594..>1064
                     /locus_tag="OQS60_004090"
     CDS             594..>1064
                     /locus_tag="OQS60_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2389687.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          279 a          231 c          287 g          267 t
ORIGIN      
        1 cgaattggtg cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag
       61 gatcgccgca tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgt
      121 aatggttggg aaaacaatgt gaaatctatt gatgccctgc catacgttga agcgggttca
      181 aacgcccgaa ccagcgatat tagcagcgga gaatatgccg ttatgccact ggcaccaatg
      241 aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacccct
      301 cgcgcgcagt atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt
      361 acctttgatg cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg
      421 ggcagccagg cagggagcaa atggatgagt gacgacctgt acgatcgagc atcaagccaa
      481 gataaacgct atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc
      541 tatgtggctg aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac
      601 atattcaaat ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac
      661 caaattttga tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt
      721 gtaaggagca gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatctcg
      781 ttctcgctta tgacgccagt ttccagggag aatctggtgg ggagcctcgc tggatagaag
      841 atccgacaca gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat
      901 atgttaatgc tgaacgtatt ggcgtgctgg gagtgtgtgg gggtggtggc tatgccatta
      961 atgccgcgct gacagaaaaa cgaattaaag cagtagtgag tattacgggt gttaatatcg
     1021 ggcgtctttt tcgggaaggt ttcagcaatt acgatccgat tggc
//
LOCUS       DAJVYM010000323         1051 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVYM010000323 DAJVYM010000000
VERSION     DAJVYM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.328"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..437
                     /gene="yadL"
                     /locus_tag="OQS60_004091"
     CDS             <1..437
                     /gene="yadL"
                     /locus_tag="OQS60_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCS2389688.1"
                     /translation="ISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTK
                     RATCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTIS
                     NAANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            464..>1051
                     /locus_tag="OQS60_004092"
     CDS             464..>1051
                     /locus_tag="OQS60_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2389689.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVY"
BASE COUNT          318 a          229 c          225 g          279 t
ORIGIN      
        1 acatttctgt tgttgacttt ggcgatgtat atatttctga aatcaatgcc aagaccaaag
       61 ttaaaacatt caaactcaaa ttcaaagact gtgcgggtat ccccaataaa aaagcacaaa
      121 taaaattaac caagcgagcc acatgcgaag gtactgctaa tgacggtgcg gggctcgcaa
      181 atggatccac agccgcagat aaagcaagtg ccgtcgccgt tgaagtctgg agcaccgaaa
      241 ctccggcaac agggagcgca acacaattta gttgtgtgac accagcatct caagaggtca
      301 ctatctccaa tgcagccaac gcggttgttt attatccgat gagtgctcga ctggtcgtcg
      361 aaaaaaataa aaccgtaagc aatgtcactg cgggtaaatt ttctgcacct gccacattta
      421 cagtaaccta taactaacag actgacagat ccaggaacta tatatgcatc ccactcaacg
      481 taagctgatg aagagaataa ttctgtttct gtcactacag tttcccatta cctatccagc
      541 catcgctggg caggatattg accttgttgc caatgtaaaa aacagcactt gcaaaagcgg
      601 aatcagtaac cagggtaata ttgatcttgg cgtcgttggg gtgggatatt tttcagataa
      661 tgttactcct gaaagctatc aaccaggtgg aaaagagttc actgtcacag tatcagactg
      721 tgcattacag ggaactggcg atgtgcttaa tcagttacat attgatttta gagcccttag
      781 cggtgtcatg gctgcaggtt caaggcaaat atttgccaat gaagtcgcaa ccggggctaa
      841 aaatgtcggt gtcgttctct tttccattca ggacccgacc aatatattca atgttattag
      901 ttcagcagga aattctcgtt ccgtgtatcc agtaatgact tctgccctgc ataattcttc
      961 atggaaattt tatgccagaa tgcaaaaaat cgatcccgcg ctggatgtca tatctggcca
     1021 agtaatgagt catatattag ttgatgtgta c
//
LOCUS       DAJVYM010000324         1013 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVYM010000324 DAJVYM010000000
VERSION     DAJVYM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.329"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            261..>1013
                     /locus_tag="OQS60_004093"
     CDS             261..>1013
                     /locus_tag="OQS60_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389690.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGANFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDT"
BASE COUNT          384 a          190 c          153 g          286 t
ORIGIN      
        1 cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt gatagttgtt
       61 aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat taccgggtgg
      121 agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta catttattgt
      181 gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta gcataaaaca
      241 aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata aatacatatc
      301 acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca gacacaccga
      361 agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc tctggagaag
      421 gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt aatgcgtgcc
      481 acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa cttgtaagtc
      541 ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca caatcaatac
      601 ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt cgaatcattg
      661 ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta gaagagggaa
      721 tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt gctgaacaac
      781 gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa atgacgaagt
      841 tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga gccaacttta
      901 ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg tcaaggtttt
      961 atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac act
//
LOCUS       DAJVYM010000325         1013 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVYM010000325 DAJVYM010000000
VERSION     DAJVYM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.330"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..853
                     /gene="ynaI"
                     /locus_tag="OQS60_004094"
     CDS             <1..853
                     /gene="ynaI"
                     /locus_tag="OQS60_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS2389691.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT          266 a          228 c          223 g          296 t
ORIGIN      
        1 tattaattat gttatagaaa actataaact caaatttatc accccagacg ttatcgattt
       61 tatctgtacg tccctgattg cggttattct gacgattaag ctatttttgc tgattaatca
      121 gtttgaaaaa cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg
      181 cattatcaaa atcaccatta ttgtggtgct tgttctgctt tatggcgaac atttcggcat
      241 gagcctttct ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg
      301 taaagatatt ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag
      361 tattggcgac tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg
      421 ctggcgaatt accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct
      481 gttttcgtcg atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac
      541 cattggttta cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga
      601 gatgctgaaa aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca
      661 gtttgccgac tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc
      721 tgaatggctt gctgcacagc aagacgttta tttgaagatt atcgatattg tgcagtcaca
      781 cggcgcggac tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga
      841 acaggggcgc taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc
      901 acatcaatgc ttcacaatat acatagtccg gctgtacgcc acatcttccg ggttattaat
      961 gggataacct ttcagccacg gcttgattaa tcgtccattg gtatattgat aaa
//
LOCUS       DAJVYM010000326         1003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVYM010000326 DAJVYM010000000
VERSION     DAJVYM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.331"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>1003
                     /locus_tag="OQS60_004095"
     CDS             <1..>1003
                     /locus_tag="OQS60_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCS2389692.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYWS
                     "
BASE COUNT          294 a          222 c          253 g          234 t
ORIGIN      
        1 cagtggtaac ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac
       61 attaacgtca ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg
      121 agcgatgaca ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt
      181 agatataacg ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg
      241 gtcatcattg aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat
      301 atcaggcaat ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt
      361 gccctatgtg caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc
      421 cactgttaca ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca
      481 tcaagctggt ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg
      541 tacagtatca gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggggttcac
      601 cggggcgtat tatcagttga ataatgacaa ctttgcccca ggaaaaacgg cggctgatta
      661 tgagttttca agctctgcct cctgggtcga tgttgatgct accggtaaag tgacatttaa
      721 aaatgtcggc agcaattcgg aaaggattac ggcgacacca aaatcaggag gccctagcta
      781 tgtatacgaa atccgtgtga agagttggtg ggtgaacgcc ggcgaggctt tcatgatata
      841 cagccttgct gaaaattttt gcagcagcaa tggctacacg ctccccagag caaactattt
      901 aaaccacagt agttcccgag gcatcgggtc actgtacagt gaatggggag atatggggca
      961 ttacacgact gaagctggtt ttcaatcaaa tatgtattgg tca
//
LOCUS       DAJVYM010000327          990 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVYM010000327 DAJVYM010000000
VERSION     DAJVYM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.332"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..615
                     /locus_tag="OQS60_004096"
                     /pseudo
     CDS             1..615
                     /locus_tag="OQS60_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          369 a          182 c          167 g          272 t
ORIGIN      
        1 acatgttcca ggttaatttt gttagttcaa cacaaaacgc cacaatctcc tgggattatt
       61 tactaaaaat aacgcctgtt ttaataagca ttagcgatat gtattctgaa gaaaaaatca
      121 agtttgtcga aagttgttta aatgagcttg gagacattac cgaagaacaa ttaaaaatta
      181 tgagatttgc aattataaaa tctataccaa gggcaactct tacagataaa ttagaaaatg
      241 aattaacaaa agaaatatat aaaagctcat cgaaaatcaa caattgcttg aacagaatta
      301 aattaccaga gatgaaagaa ttctcatcag aaaaaaatat atgattacat cgatataatc
      361 attgaagatt atgaaaatat caaagaaaat gcttatctgg tcgatccaaa aattaattac
      421 actatagatt taaacataga agactctagc tcagaggaat tcctttttga taatagcctc
      481 gagaaagacg aaaattcttc ggacaattgc tttgaggtcg aaaaatataa cgcatatgaa
      541 gcatataact cagagaatct atattttacc agagaggaat atacgtatga ttacgacctt
      601 ttaaatgcaa tatagacata ataacttctt acaaaatcta taattaatac cgtagagatt
      661 cattcatctt accctgtgca ccaaaagcaa aaactcactg acgcgggttt gaaactgatg
      721 gcaaccgcaa aaatgcctga tgcgctacgc ttatcaggcc tacgccatct ctacaatata
      781 ttgaatttgc gtgcttttgt aggcaggata aggcgttcac gcagcatccg gcatgaataa
      841 agcgcactca acaatctgaa acccgccgga acggtttatt acgtacatca ggtgaaactg
      901 accgataagc cgctttcttt tgggtatagt gtcgtggaca gtcattcatc tttctgcccc
      961 tccaaaagca aaaacccgcc gaagcgggtt
//
LOCUS       DAJVYM010000328          983 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVYM010000328 DAJVYM010000000
VERSION     DAJVYM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.333"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..304)
                     /locus_tag="OQS60_004097"
     CDS             complement(<1..304)
                     /locus_tag="OQS60_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS2389693.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(343..630)
                     /locus_tag="OQS60_004098"
     CDS             complement(343..630)
                     /locus_tag="OQS60_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389694.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(880..>983)
                     /locus_tag="OQS60_004099"
     CDS             complement(880..>983)
                     /locus_tag="OQS60_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS2389695.1"
                     /translation="IVTALVTRKDLCEVRIRTGQTEVAVFVDYESRK"
BASE COUNT          257 a          262 c          222 g          242 t
ORIGIN      
        1 gctctgcggc ttcgctcaga gctggcgtat gttcccggcc tactgattca cacaggtaat
       61 caacgaaacc ggacaccagt tttcgttcgc gaggcgtgat cgccccaccg accggagtcc
      121 agtaatcgaa acccagttgc aggagtttga aaaaacgctt gtggaatgcg tagttacaca
      181 cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaga aaatcgcaac
      241 tctccggcgt cgccgggaga agtaatccgg aagaggtttg tttgaccagt tgtatatgtg
      301 ccatcggctt tctccggtgg cacggtgtta cacagcagga gttcaatcct gctcaagatt
      361 gtagatgagt ttattcttca gcaaaagcag aaaaccagcc ttaaaaccaa tctctttcaa
      421 aacccgtaat gatgtgacaa attcgtcctc acgcaaaata aaaccgtccg tcagaagtcc
      481 attacaaaaa taaaataaca cagcaccgct cttcctttgt tgagattgca aacatctaat
      541 gcggcaatgg ctgacaatcg ctccattctc aacgcgcaca gcatagaggc cattttcact
      601 aaaaatttca cgcaattctt cgattttcat cttcagaatc cttccagata aatagctctc
      661 ccctgttcgg ggtccatccc tcttctccct gcgcgctact taagtgagtc gattctatct
      721 gcgaaggtgc gcgaatcaaa ttcaccggaa ataaacaaca aaaaacccgc cgaaacgctg
      781 agagatcaca gcgatgggcg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga
      841 ggtgggcggg gatgggatca gctccccgcc cggtcactct tacttcctgg attcgtagtc
      901 tacgaagaca gcgacctccg tctggccggt tcggattcgt acctcacaga ggtctttcct
      961 cgttaccagt gccgtcacta tga
//
LOCUS       DAJVYM010000329          983 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVYM010000329 DAJVYM010000000
VERSION     DAJVYM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.334"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(220..983)
                     /locus_tag="OQS60_004100"
                     /pseudo
     CDS             complement(220..983)
                     /locus_tag="OQS60_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          318 a          138 c          190 g          337 t
ORIGIN      
        1 ttgaaagaat agataagaaa gcgtaaccgc gggggcagaa tggggattaa gtagccagat
       61 atgtgttact aaatgtaact aacaagctaa ccttcaaacg gggttaatct ttgaacgtat
      121 gtcatgtttc aacccttcag atcgtgaatc taaagggtta catattaact atattatcaa
      181 caagaccaga cacttataga attagtgatg atttattcac taaaaaggcc atgaactgga
      241 ggaaaacgat ctgtccagaa ggtctatcgg ttcctctctt attgcctgat tgtaataatc
      301 gttattacag taagtattgt ttatttcatc taaggtcatg tttttaactt ttatattcct
      361 ctggtttaca acttcctctg tcttgtaagt gtgtatatta tatggggtat atgctatttt
      421 taataggata gtgaaacaat tcgttatcca catctattcc gtcagagtga acttcaaatg
      481 caccaggcga aagaattgcc aggccagtat taagcttaag tgttggggaa aacaaattaa
      541 ataacatgag ctgcatagta gggcaattgt tcttcccaga aacatttaaa aaatttaagt
      601 ttttgctctg ttttattatg ggaaataaag ttccaatatc ggatgtatta ttagtatcaa
      661 tgtgggttat tttattgttg tccagtctta aacttttaat agatggaaga caagtcagga
      721 aatcaagatt tatatttttc aactggttgt tagcagcgtt taatgtctga acattgcaga
      781 agttgctata gaatatttta atttcagaaa gcaggttttt gttaataagt aacgtattca
      841 agttgggaaa taagacattg tgtaaacttg ttagtttatt atgggataga tccagcagta
      901 agagttaatt tttattacct gcaacaatat cagataattg attatggctg agattcaggt
      961 attgcagaca ttcgcaagat tct
//
LOCUS       DAJVYM010000330          979 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVYM010000330 DAJVYM010000000
VERSION     DAJVYM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.335"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            533..>979
                     /locus_tag="OQS60_004101"
     CDS             533..>979
                     /locus_tag="OQS60_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS2389696.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQ"
BASE COUNT          229 a          251 c          264 g          235 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacag
//
LOCUS       DAJVYM010000331          957 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVYM010000331 DAJVYM010000000
VERSION     DAJVYM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.336"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..614)
                     /locus_tag="OQS60_004102"
     CDS             complement(<1..614)
                     /locus_tag="OQS60_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCS2389697.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNS"
     gene            complement(661..867)
                     /locus_tag="OQS60_004103"
     CDS             complement(661..867)
                     /locus_tag="OQS60_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389698.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT          270 a          252 c          192 g          243 t
ORIGIN      
        1 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
       61 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
      121 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
      181 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
      241 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
      301 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
      361 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
      421 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
      481 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
      541 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
      601 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
      661 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
      721 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
      781 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
      841 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
      901 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgct
//
LOCUS       DAJVYM010000332          947 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVYM010000332 DAJVYM010000000
VERSION     DAJVYM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.337"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(14..>947)
                     /gene="flhB"
                     /locus_tag="OQS60_004104"
     CDS             complement(14..>947)
                     /gene="flhB"
                     /locus_tag="OQS60_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCS2389699.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT          220 a          287 c          238 g          202 t
ORIGIN      
        1 catcgcggcc agattactca tgggtcggtt tctcgttaat aaaatccagg gcttccggca
       61 ccggaagatg agtaggttgt acagggcgct gtccaccagc cagacgccag cgtttcagtt
      121 gccagaccca ggccagcact tccgccaccg cggcgtacag ttgacccggg atttgttgac
      181 caatctccgc atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt
      241 tattttcagc gccaatttca cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca
      301 ctttcggtgc gctcattttg ttttcgtcat actgcaacgc taccgaatag tgggtcgggt
      361 tattgacaat gacatcagct ttcggcacat cggccatcat ccgacgccgt gcagcagctc
      421 gctgcatctg acggatccgc cctttaacat gagggtcacc ttcgctttgt ttgaactcat
      481 cacgaatatc ctgccgcgac atacgcagct ttttcaggtg gctgaagatt tggaaaaaga
      541 cgtcaaatcc caccattgga atgacaccaa gcaccaccag cagtgcgcat agcccaacca
      601 gatccatcgc attacccatg gcggtagtcg gagactcggc cattaagcgc atcatctgcg
      661 gccagtgatg ccagagataa aaccccgtca cgctgccaac caggatggtt ttcaaaattg
      721 ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg tttaatgccc ggtagcgggt
      781 tgagtttgga aaacttcggc tgcaaggatt tgccgctaaa taccagcccc cccagcatga
      841 ccggagaaat gagcgccacc agcaccacgc cgctaatcag cggcagcagc gccagcatgg
      901 cttctctgat cagcagaata atctgcccga ggatcagatt cgggtca
//
LOCUS       DAJVYM010000333          945 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVYM010000333 DAJVYM010000000
VERSION     DAJVYM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.338"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>945)
                     /gene="cadA"
                     /locus_tag="OQS60_004105"
     CDS             complement(<1..>945)
                     /gene="cadA"
                     /locus_tag="OQS60_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCS2389700.1"
                     /translation="HRALERLNFQIVYPNDRDDLLKLIENNARLCGVIFDWDKYNLEL
                     CEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISFFEYALGAAEDIANKIKQTTDEY
                     INTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQKSPVGSLFYDFFGPNTMKSDISI
                     SVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTNGTSTANKIVGMYSAPAGSTILI
                     DRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIPQSEFQHATIAKRVKETPNATWP
                     VHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWVPYT"
BASE COUNT          252 a          201 c          236 g          256 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg tagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatga
//
LOCUS       DAJVYM010000334          942 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVYM010000334 DAJVYM010000000
VERSION     DAJVYM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.339"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            192..>942
                     /locus_tag="OQS60_004106"
     CDS             192..>942
                     /locus_tag="OQS60_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCS2389701.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQ"
BASE COUNT          207 a          291 c          246 g          198 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      781 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      841 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      901 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gc
//
LOCUS       DAJVYM010000335          934 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVYM010000335 DAJVYM010000000
VERSION     DAJVYM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.340"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(195..>934)
                     /locus_tag="OQS60_004107"
     CDS             complement(195..>934)
                     /locus_tag="OQS60_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2389702.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          280 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga ccaa
//
LOCUS       DAJVYM010000336          923 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVYM010000336 DAJVYM010000000
VERSION     DAJVYM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.341"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..229
                     /gene="cysM"
                     /locus_tag="OQS60_004108"
     CDS             <1..229
                     /gene="cysM"
                     /locus_tag="OQS60_004108"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="catalyzes the formation of cysteine from
                     3-O-acetyl-L-serine and hydrogen sulfide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS2389703.1"
                     /translation="VLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPG
                     AVVVAIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(232..600)
                     /locus_tag="OQS60_004109"
     CDS             complement(232..600)
                     /locus_tag="OQS60_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS2389704.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            705..>923
                     /gene="pdxK"
                     /locus_tag="OQS60_004110"
     CDS             705..>923
                     /gene="pdxK"
                     /locus_tag="OQS60_004110"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="catalyzes the formation of pyridoxal 5'-phosphate
                     from pyridoxal; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS2389705.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWF"
BASE COUNT          219 a          199 c          263 g          242 t
ORIGIN      
        1 ggtgctggat attcatcagc gcgatgcaga aaacaccatg cgcgaactgg cagtgcggga
       61 aggaatattc tgtggcgtca gctccggcgg cgcggttgcc ggagcactgc gggtggcaaa
      121 agctaaccct ggtgcggtgg tggtagcgat catctgcgat cgtggcgatc gctacctttc
      181 caccggggtg tttggggaag agcattttag tcagggggcg gggatttaag attattgcgc
      241 aggcaccgtt ttatccgtct gcgcaattaa ggcatgtaaa aaaggctgtg cgttctcatc
      301 gctcttaccg gggatcttca caatatacgg cttcccggta atcgatgacg acgaagcaac
      361 cttatcaata aactgctcgg cagtgtcaat gcggttacgg gtattgccga gcttcagacg
      421 cagatgagaa accgcttcat cgcaggtatg ttcatcaccg ttgcgcacaa atatcaaatc
      481 ctttttctgt gctaatccct ccagcatggc gttgatgcgg gcttcttcat gaacagttaa
      541 cttcgcgtaa gcggggagtg tcattagtag tgtaatgacc agacaaatga ttttcttcat
      601 tgaattacct tatccttgtt ttgtgatagc cgatttgact gccagaatca ggtaaagttt
      661 cggttggaat acgatgagga tcatcggcaa ggagaaaaaa ttaaatgagt agtttgttgt
      721 tgtttaacga taagagtagg gcgctgcagg cggatatcgt cgccgtgcag tcgcaggtag
      781 tgtacggcag cgtaggcaac agcattgcgg tgcctgctat aaaacagaac ggcttgaatg
      841 tctttgccgt gccgacggta ttgctgagca atacgccgca ttatgacact ttctacggtg
      901 gtgcgattcc ggacgaatgg ttt
//
LOCUS       DAJVYM010000337          916 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVYM010000337 DAJVYM010000000
VERSION     DAJVYM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.342"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..541
                     /locus_tag="OQS60_004111"
     CDS             <1..541
                     /locus_tag="OQS60_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS2389706.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
     gene            complement(597..>916)
                     /locus_tag="OQS60_004112"
     CDS             complement(597..>916)
                     /locus_tag="OQS60_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2389707.1"
                     /translation="YSLEKQRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLL
                     CDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHI
                     LAA"
BASE COUNT          268 a          198 c          213 g          237 t
ORIGIN      
        1 gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag cgaaggaata
       61 tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta cttgtcgaaa
      121 agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg ttacccgttc
      181 tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc tcttcgccac
      241 caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc
      301 cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat
      361 tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa
      421 accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc
      481 aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta
      541 acgctgctca gcccccggct aaggcaaaac ttgccctggc gggtggcgat aataatctaa
      601 gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc
      661 gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca
      721 agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca
      781 catagtaggt cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact
      841 aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aacgcgttgt
      901 ttttccagcg aatatg
//
LOCUS       DAJVYM010000338          909 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVYM010000338 DAJVYM010000000
VERSION     DAJVYM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.343"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..294)
                     /locus_tag="OQS60_004113"
     CDS             complement(<1..294)
                     /locus_tag="OQS60_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2389708.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(342..902)
                     /locus_tag="OQS60_004114"
     CDS             complement(342..902)
                     /locus_tag="OQS60_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2389709.1"
                     /translation="MIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTADQ
                     IVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGT
                     PLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIA
                     RYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          258 a          214 c          188 g          249 t
ORIGIN      
        1 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
       61 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
      121 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
      181 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
      241 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      301 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      361 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
      421 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
      481 ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
      541 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
      601 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
      661 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
      721 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
      781 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
      841 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
      901 atattacgc
//
LOCUS       DAJVYM010000339          908 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVYM010000339 DAJVYM010000000
VERSION     DAJVYM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.344"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..348)
                     /gene="aroF"
                     /locus_tag="OQS60_004115"
     CDS             complement(<1..348)
                     /gene="aroF"
                     /locus_tag="OQS60_004115"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS2389710.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDG"
     gene            557..>908
                     /locus_tag="OQS60_004116"
     CDS             557..>908
                     /locus_tag="OQS60_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS2389711.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT          259 a          199 c          209 g          241 t
ORIGIN      
        1 gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc
       61 aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa
      121 tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac
      181 cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat
      241 ctgggcttct tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa
      301 aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct
      361 gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa
      421 gtaaagtttt caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt
      481 ttgatgctta tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt
      541 gaaaaaggag aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag
      601 cggatgtcag attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga
      661 tgtcggtatg gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga
      721 ttcacaggcg gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg
      781 ccagaccgat tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa
      841 caatgttgaa agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc
      901 cagcacaa
//
LOCUS       DAJVYM010000340          908 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVYM010000340 DAJVYM010000000
VERSION     DAJVYM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.345"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            5..424
                     /locus_tag="OQS60_004117"
     CDS             5..424
                     /locus_tag="OQS60_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS2389712.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            424..>908
                     /gene="umuC"
                     /locus_tag="OQS60_004118"
     CDS             424..>908
                     /gene="umuC"
                     /locus_tag="OQS60_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS2389713.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
BASE COUNT          209 a          198 c          249 g          252 t
ORIGIN      
        1 tattatgttg tttatcaagc ctgcggatct ccgcgaaatt gtgacttttc cgctatttag
       61 cgatcttgtt cagtgtggct ttccttcacc ggcagcagat tacgtcgaac agcgcatcga
      121 tctgaatcaa ctgttgatcc agcatcccag cgcgacttac ttcgtcaaag caagtggtga
      181 ttctatgatt gatggtggaa ttagtgacgg tgatttactg attgtcgata gcgctattac
      241 cgccagccat ggtgatattg tcatcgctgc tgttgacggc gagtttacgg tgaaaaaatt
      301 gcaactacgc ccgacggtac agcttattcc catgaacagt gcgtactcgc ccattaccat
      361 cagtagcgaa gatacgctgg atgtctttgg tgtggtgatc cacgtcgtta aggcgatgcg
      421 ctgatgtttg ctctctgtga tgtaaacgcg ttttatgcca gctgtgagac ggtgtttcgc
      481 cctgatttat ggggtaaacc ggtggttgtg ctatcgaata atgacggttg cgttatcgcc
      541 cgaaacgctg aggcaaaggc gctgggtgtt aaaatgggcg atccctggtt caaacaaaaa
      601 gatctgtttc gtcgctgtgg tgtggtttgc tttagcagca attatgagct ttacgcagac
      661 atgagcaatc gggtgatgtc gacgctggaa gaactatcgc cccgcgtcga gatttacagt
      721 attgatgagg cattctgcga tctgacaggt gtgcgtaatt gtcgcgatct gactgatttt
      781 ggcagagaaa ttcgcgcaac ggtgctacaa cgtacccatc ttactgttgg tatggggatc
      841 gcccagacca aaacgctggc taagcttgcc aatcatgcgg caaaaaaatg gcagcggcag
      901 acgggggg
//
LOCUS       DAJVYM010000341          886 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVYM010000341 DAJVYM010000000
VERSION     DAJVYM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.346"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(68..733)
                     /locus_tag="OQS60_004119"
     CDS             complement(68..733)
                     /locus_tag="OQS60_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS2389714.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          227 a          221 c          201 g          237 t
ORIGIN      
        1 tcaagcaccc gcagtcattc tacttacctc gccaatatga aatcaatcag aaaggtgccc
       61 cataaaatca ctccttctct tctttaccgt agtggagttg accaattttg ataagagggc
      121 gtccctgaga tttgcggtgt agattggtat cgcgcagaga atacacacag ccacaatatt
      181 cctgctgata gaatttttcg cgcttgctga tttcaatcat acgggacgag ccgccctgct
      241 tgcgccagtt ataatcccag tacaccatgc ccggataatg cgcgacggct cgccgcccac
      301 agtcgttaac ctgctgcata tttttccagc gtgaaatgcc cagtgaactg ctgatcacac
      361 tgaaaccatt ttcagcggcg tacagcgctg tccgctcaaa acgcatgtca aaacacatgg
      421 tgcaacggat ccctcgttcg ggctcccatt ccattccttt ggctcgttca aaccagttgt
      481 ctgtgtcgta atcagcatca ataaacggca cgccgtgttg ttcagcaaag cgaatattct
      541 catccttacg aattaaatac tctttctgag gatgaatgtt cgggttgtag aaaaagatgg
      601 tgtagtcgat tcccgaggcc tgaagcgcct ccatcacctc accggaacat ggagcacagc
      661 aagagtgcag tagtagtttg tttgccccgt ttgggagctc caatttgggc cgtttgaaat
      721 cagcaactgt cataaatatg tttattgggg tcataaaaat atcaaagagt gtagcattag
      781 aacggggcta tcggaaacag atgtgtgact gctccccgcc ctttcgggtg gtctcctgat
      841 gatttgaggg tgcagaaatc cctccggtta aggattaaat ttttaa
//
LOCUS       DAJVYM010000342          873 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVYM010000342 DAJVYM010000000
VERSION     DAJVYM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.347"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..646
                     /gene="fimH"
                     /locus_tag="OQS60_004120"
     CDS             <1..646
                     /gene="fimH"
                     /locus_tag="OQS60_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCS2389715.1"
                     /translation="QNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQ
                     VMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAG
                     PGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQND
                     TPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            657..>873
                     /locus_tag="OQS60_004121"
     CDS             657..>873
                     /locus_tag="OQS60_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389716.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVP"
BASE COUNT          206 a          191 c          230 g          246 t
ORIGIN      
        1 gcaaaattat attcggatgg gaacagatcc taacgtttcg caaaaccttc cgttcggggt
       61 gatggattct cgtttgatat ttcgtttaaa ggttattcgt ccctttatta acatggtgga
      121 gatccccaga caggtgatgt ttaccgtgta tgtgacatca acgccttacg atccgttggt
      181 tacacctgtt tataccatta gttttggtgg ccgggttgaa gtgccgcaaa actgcgaatt
      241 aaatgccggg cagattgttg aatttgattt tggtgatatc ggcgcatcgt tatttagtgc
      301 ggcagggccg gggaatcgac cagctggcgt catgccgcaa accaagagca ttgcgatcaa
      361 atgtacgaat gttgctgcgc aggcttattt aacaatgcgt ctggaagcca gtgccgtttc
      421 tggtcaggcg atggtgtcgg acaatcagga tttaggtttt attgtcgccg atcagaacga
      481 tacgccgatc acgcctaacg atctcaatag cgttattcct ttccgtctgg atgcagctgc
      541 ggcagccaat gtcacacttc gcgcctggcc tatcagtatt accggtcaaa aaccgaccga
      601 agggccgttt agcgcgctgg ggtatttacg cgtcgattat caatgaggta cggagaatga
      661 gaagagtact ctttagctgt ttctgcgggt tactgtggag ttccagtgga tgggcagctg
      721 accctttggg aacgattaat atcaatttgc acggtaacgt tgttgatttc tcctgtaccg
      781 taaacactgc ggatattgat aagacggtag atttaggcag atggcctacg acacaattac
      841 tgaacgctgg cgataccacg gcactcgtcc ctt
//
LOCUS       DAJVYM010000343          846 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVYM010000343 DAJVYM010000000
VERSION     DAJVYM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.348"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..196
                     /locus_tag="OQS60_004122"
     CDS             <1..196
                     /locus_tag="OQS60_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389717.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="OQS60_004123"
     CDS             207..374
                     /locus_tag="OQS60_004123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389718.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            482..715
                     /locus_tag="OQS60_004124"
     CDS             482..715
                     /locus_tag="OQS60_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389719.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
BASE COUNT          247 a          160 c          236 g          203 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttga
//
LOCUS       DAJVYM010000344          832 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVYM010000344 DAJVYM010000000
VERSION     DAJVYM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.349"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>832
                     /locus_tag="OQS60_004125"
     CDS             <1..>832
                     /locus_tag="OQS60_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCS2389720.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     R"
BASE COUNT          184 a          220 c          226 g          202 t
ORIGIN      
        1 tccgctattg ccagtacacc tcagtcgggt tgccaatctc gaatttaatc agttcccccc
       61 taccgcactg tatcacccac aggcacgcaa tttcctgtta acggaagcac tgcgcggcga
      121 aggcgcttat ctcaagcgcc cggatggcac gcgttttatg cccgattttg atgagcgcgg
      181 cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac catgaaatga aacgcctcgg
      241 cgcagattgt atgttcctcg acatcagcca taagcccgcc gattttattc gccagcattt
      301 cccgatgatt tatgaaaaat tgctcgggct ggggattgat ctcacacaag aaccggtacc
      361 gattgtgcct gctgcacact atacctgcgg tggtgtaatg gttgatgatc atgggcgtac
      421 ggacgtcgag ggcttgtatg ccattggcga ggtgagttat accggcttac acggcgctaa
      481 ccgcatggcc tcgaattcat tgctggagtg tctggtctat ggctggtcgg cggcagaaga
      541 tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg ttaccaccgt gggatgaaag
      601 ccgcgttgag aaccctgacg aacgggtagt aattcagcat aactggcacg agctacggct
      661 gtttatgtgg gattacgtcg gcattgtgcg cacaacaaaa cgtcttgagc gcgccctgcg
      721 gcgtatcaat atgttgcaac aggaaataga cgaatattac gcccacttcc gcgtgtcgaa
      781 taatttgctg gagctgcgta atctggtaca ggttgccgag ttgattgttc gc
//
LOCUS       DAJVYM010000345          830 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVYM010000345 DAJVYM010000000
VERSION     DAJVYM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.350"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(25..315)
                     /locus_tag="OQS60_004126"
     CDS             complement(25..315)
                     /locus_tag="OQS60_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS2389721.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="OQS60_004127"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="OQS60_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS2389722.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
BASE COUNT          218 a          229 c          171 g          212 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt
//
LOCUS       DAJVYM010000346          825 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVYM010000346 DAJVYM010000000
VERSION     DAJVYM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.351"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..516)
                     /locus_tag="OQS60_004128"
     CDS             complement(<1..516)
                     /locus_tag="OQS60_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS2389723.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          267 a          173 c          152 g          233 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagc
//
LOCUS       DAJVYM010000347          814 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVYM010000347 DAJVYM010000000
VERSION     DAJVYM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.352"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            229..>814
                     /locus_tag="OQS60_004129"
     CDS             229..>814
                     /locus_tag="OQS60_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2389724.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          226 a          180 c          174 g          234 t
ORIGIN      
        1 cccaccggca gaagcagaaa aagcttatta tgcttccatc ggaaatgatg atctggcagc
       61 ctgagttcac agataaaaca ctctccagga aacccggggc ggttcagttc acaaaaacac
      121 attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca ttagcttctt
      181 cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt
      241 tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc
      301 agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg cattccagca
      361 atctgtctgt gttactctca gatatccctg gcgggtattg tccgttccga tattgcctat
      421 cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc caatgatatt
      481 ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc aatggccgat
      541 ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta tatcgtcagc
      601 gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa tacatattcc
      661 cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct gaataaactg
      721 gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa tgcttataaa
      781 tacactgaac gttacaccgc tttttatcgg gtgg
//
LOCUS       DAJVYM010000348          756 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVYM010000348 DAJVYM010000000
VERSION     DAJVYM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.353"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..715)
                     /locus_tag="OQS60_004130"
                     /pseudo
     CDS             complement(1..715)
                     /locus_tag="OQS60_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
BASE COUNT          230 a          163 c          129 g          234 t
ORIGIN      
        1 cttggcctgc gccataggct ttaaatgaat acagataatt tgatgagctg gataatttga
       61 tctgttgagt tactgttgga tcaaagtttg tatctgtata gaattcaatt gtaagggtct
      121 gataaatacc acctacagcc cagtatttac gggtgctatt cgcattattg caactccaat
      181 ataacacgtc ttcgcttgga ttataccacg aaaaatattg ttctgcagag tcaccaatgt
      241 atatcccagg tgaactaacg ttagttacgg taccgtaagc tgaccacatg tttgaaatta
      301 ataatgtgta atacaaacca ggaactgatg tattaaataa tttatgggag ccatacattt
      361 tacctgagtc aaccattgca ttttcaatac gcattgcgct accacttgtg ctatcacttc
      421 tgttgcaata aagaaaacca ccaccagaag cagtatctaa ctgactctac tgtaatttta
      481 cacctgtagg tccaagctgc gtcggtttgt agaaaactaa attatttgta cttgttgcat
      541 atctgaatat gacagtagca tcgccattcg gccccgtttc tctccatttt tgatctgacg
      601 gaccaacgcc gtaactattc ccaacaatat atcctacgtt atcatcagtg ccagcactcg
      661 cggtataaca actcaagcac agaattaaaa aaaaatgtct gaagaatttc ttcattatca
      721 ctccaccatc tactgctgta acagtcgcag ttattt
//
LOCUS       DAJVYM010000349          752 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVYM010000349 DAJVYM010000000
VERSION     DAJVYM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.354"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..594)
                     /locus_tag="OQS60_004131"
     CDS             complement(<1..594)
                     /locus_tag="OQS60_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2389725.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVAR"
     gene            complement(634..>752)
                     /locus_tag="OQS60_004132"
     CDS             complement(634..>752)
                     /locus_tag="OQS60_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS2389726.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          177 a          198 c          183 g          194 t
ORIGIN      
        1 tctcgcgact aacgcttcat ttacatcaaa ttccagtagg gtgccgtcat gttcaatggc
       61 acctatactg gtgattaaat aatctgcgcg gaagccttca ataaaatcca cggctcctgg
      121 gccgataatc ccgccgttat gagcgcgtaa agtgcctccc ggcaccatca cttcaatatc
      181 ctgattctta taaagaatct gtgccacacg taggctgtta gtgataatgc gtaaatcacg
      241 ccggttgagt aatgccctgg caacggcttc tacggttgta ccgatggtga taaagacggt
      301 acagcgttcg ggaaggtaat cggcgactgc ctcagcgatc gcccgtttct cggcggtgag
      361 cgaaagttcc cgttgctcaa aagcggtatt catgacgctg gagacgcgcc cggcccctcc
      421 atgatgacgg gtgatcaagc cctgctcact gagttttcgg atatcccggc gcacggtttg
      481 cgttgaaaca tccagcagtt gtgccagttc ttcaatattc atataaccgc gttctgcgat
      541 tagatgaacg agttggtcat ggcgtggatt acctgtcact tcgatgatac tcatgaatac
      601 tcccttgccc ctcctcggta gatgtttacc attttacgca taaagtgaca aaaatgattc
      661 ggtttgctca cgattaggaa tgccggcccg accgccaggc tgggtacatt tcatcgcggc
      721 aaccgcactg gcaaaacgaa tagcctcttt tg
//
LOCUS       DAJVYM010000350          742 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVYM010000350 DAJVYM010000000
VERSION     DAJVYM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.355"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..659
                     /gene="baeR"
                     /locus_tag="OQS60_004133"
     CDS             <1..659
                     /gene="baeR"
                     /locus_tag="OQS60_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS2389727.1"
                     /translation="GQLLIDYLRAASYAPTLISHGDQVLPYVRQTPPDLILLDLMLPG
                     TDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEIGADDYICKPYSPREVVARVKTI
                     LRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLDLTPAEFRLLKTLSHEPGKVFSR
                     EQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAEQSFIRAVYGVGYRWEADACRIV
                     "
BASE COUNT          169 a          205 c          196 g          172 t
ORIGIN      
        1 tggggcagtt gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc
       61 acggcgatca ggtactgccg tatgtgcgcc agacaccacc ggatctgatc ctgttggatc
      121 tgatgctccc tggcaccgat ggcttgacgc tgtgccggga aattcgtcgt ttttctgaca
      181 ttcctatcgt gatggtgacg gcaaaaatcg aagagatcga ccgcctgctc ggactggaga
      241 ttggcgcaga tgactacatc tgcaagccgt acagcccacg ggaagtggta gcgcgcgtca
      301 aaaccatttt gcgccgttgc aaaccgcagc gcgaattgca gcaacaggat gccgaaagcc
      361 cgttgattat cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg cttgacctga
      421 cgcctgcgga atttcgtctg ttgaaaacgc tctctcatga accaggaaaa gtgttctccc
      481 gcgagcaatt gctcaatcat ctttatgacg actaccgcgt agtaaccgac cgcaccattg
      541 acagccacat taaaaacctg cgccgcaagc tggaatctct cgacgccgaa cagtcattta
      601 tccgcgccgt ttacggcgtt ggttaccgct gggaagccga cgcctgccgc atcgtttagt
      661 tttagcgaca tgactttgtt agccggagat ggcgttcaga ccagatccgg caacattatc
      721 ccacgcatgg tcagcaaact ga
//
LOCUS       DAJVYM010000351          738 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVYM010000351 DAJVYM010000000
VERSION     DAJVYM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.356"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          219 a          128 c          131 g          260 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaaaaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattatt
//
LOCUS       DAJVYM010000352          738 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVYM010000352 DAJVYM010000000
VERSION     DAJVYM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.357"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..461
                     /locus_tag="OQS60_004134"
     CDS             <1..461
                     /locus_tag="OQS60_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS2389728.1"
                     /translation="KQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSL
                     GIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELT
                     SRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          248 a          139 c          152 g          199 t
ORIGIN      
        1 tgaagcaggc catgcaggga caggacgttg tgtatgccaa ccttacgggg gaagatttag
       61 atattcaggc aaattcagtg atcgctgcga tgaaagcctg tgatgttaaa cgtctgatct
      121 ttgttctttc cctcggtatt tatgatgaag ttccaggaaa atttggcgaa tggaataatg
      181 cagttattgg tgagccccta aaaccattcc gcagggctgc ggatgcaatt gaggcttctg
      241 gactggaata tacaattctt cgccctgcct ggctgactga tgaagatatt attgattatg
      301 agcttacctc acgcaatgaa ccattcaaag gtacgatcgt atcacgtaag agtgtcgcag
      361 cattaatcac agatatcatc gataaacctg agaaacacat tggtgagaat ataggtatca
      421 atcaacccgt gactgatggt gacaagccat tattcatgta aaccaattaa atagttacca
      481 tcgtaatgta ctatgtgcag tacgatttat taaacttatt aaggtgaact gatcaatccg
      541 atgcgattaa catgtgtaga accagtaaga ataaaatacg ccatgtatat aacattacga
      601 cttacatcta agtcagcata attctaatat ctgcacatca ataaaaatgc aaacaacttc
      661 cggcaaaaaa agtgatacaa gatcgatgac atagcgttga agaaaaggat ttgataatcc
      721 gcaaaatttg cacaacac
//
LOCUS       DAJVYM010000353          737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVYM010000353 DAJVYM010000000
VERSION     DAJVYM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.358"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>737
                     /locus_tag="OQS60_004135"
     CDS             <1..>737
                     /locus_tag="OQS60_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS2389729.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFS"
BASE COUNT          178 a          184 c          226 g          149 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctc
//
LOCUS       DAJVYM010000354          732 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVYM010000354 DAJVYM010000000
VERSION     DAJVYM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.359"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..479)
                     /locus_tag="OQS60_004136"
     CDS             complement(<1..479)
                     /locus_tag="OQS60_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS2389730.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGC"
BASE COUNT          212 a          161 c          164 g          195 t
ORIGIN      
        1 tcgcagcctt ccagggcggg tacgcaagag ctgacctgta agtactggtt ttgccttgcc
       61 tgttcggcac agcgggcaaa ccaggcgaat ccggcttctc ccatcacgtt gacgatttca
      121 tgagggtcgc aaataacggt cgtcaggccg cgcggcaggg tagcggtttc aaaagtgacc
      181 ggcgtcatca tgctggattc aatatgcagg tgagcatcaa taaaccctgg caccgccgtt
      241 gcgccgcgag catcaatccg ctgcaaagcc ggagcatcag cgtattctgc gccaacaccg
      301 gcaatgtaac gtcctttaat cacaattggg ccggaaattt ctccgccatt gatcaggtcg
      361 agaatagaga cattatcaat aatataatcg gcaacagcgt cgccacggga aacggctaac
      421 aattcctggt attcagcccg gctaatgtga tgaaatttat ggttaataga attattcata
      481 tttttctcat tttgatttta tcaatttgat tattatttat cataaagaaa cgtcgcgaga
      541 ttattaaaga atatccatta atgtgcaatt gaaatgtgat aattatcact gaaccgttgt
      601 tgtcatttgt tttacaaaag caagggatta aatctcctcc aatggacaaa aaaatgaata
      661 atgacaatac cgattacgtg agtaatgaat cagggacgct ttcgcgatta tttaaactac
      721 ctcagcatgg ga
//
LOCUS       DAJVYM010000355          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVYM010000355 DAJVYM010000000
VERSION     DAJVYM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.360"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            161..679
                     /locus_tag="OQS60_004137"
     CDS             161..679
                     /locus_tag="OQS60_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS2389731.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT          208 a          161 c          144 g          213 t
ORIGIN      
        1 gattaaatta attaaaaccc cacaaataaa ttaaacataa gattttactc attgtgtttt
       61 atttctcaca ttgatgacga tcgcatgaaa aaatgatatt ttaatgagca ttctgaaaaa
      121 atatcgatgc atttcgagcg aagatggtgg ggatccctga atgcgttttt ctcaccgact
      181 gttcttactt ctgattctcc tgctgacggg agcaccaata ctcgctcagg agccttctga
      241 cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc agttacaccc gctggcctgc
      301 gctatcaggg ccaccaaagt tatgtatctt ttcatcttca cgttttagta ccgccctaca
      361 agagaatgcc gcgacatctt tgccttatct gcctgtcatc attcacactc aacaagaggc
      421 gatgatttca ggctgcaatg gtttttattt tggcaacgag tcaccgacat tccagatgga
      481 attaaccgaa caatatccgt caaaggcgtt attattaatt gccgaacaaa ataccgagtg
      541 cattattggt agcgcctttt gtctgatcat ccacaataat gacgtcagat ttgccgtaaa
      601 cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa tgctcgcacg
      661 gaagaaaaat gatggataac gataattctc ttaataagcg ccccacgttt aaaagagcat
      721 tacgca
//
LOCUS       DAJVYM010000356          721 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVYM010000356 DAJVYM010000000
VERSION     DAJVYM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.361"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>721)
                     /locus_tag="OQS60_004138"
     CDS             complement(<1..>721)
                     /locus_tag="OQS60_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS2389732.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDL"
BASE COUNT          188 a          193 c          153 g          187 t
ORIGIN      
        1 aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc aatatatccc
       61 gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt ccagtcccat
      121 tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc tggataagca
      181 ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt gatggtcagt
      241 gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat ctgatgagtc
      301 caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc gtaagccgtt
      361 tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac agtaaaggtc
      421 tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt cacccactta
      481 ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa aatatcgaca
      541 tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc aaaaaccagc
      601 ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg cagaccaata
      661 ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat gattgctttc
      721 g
//
LOCUS       DAJVYM010000357          715 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVYM010000357 DAJVYM010000000
VERSION     DAJVYM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.362"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>715
                     /locus_tag="OQS60_004139"
     rRNA            <1..>715
                     /locus_tag="OQS60_004139"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          203 a          155 c          220 g          137 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accat
//
LOCUS       DAJVYM010000358          713 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVYM010000358 DAJVYM010000000
VERSION     DAJVYM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.363"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          245 a          109 c          148 g          211 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc tgt
//
LOCUS       DAJVYM010000359          704 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVYM010000359 DAJVYM010000000
VERSION     DAJVYM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.364"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..190
                     /locus_tag="OQS60_004140"
     CDS             <1..190
                     /locus_tag="OQS60_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS2389733.1"
                     /translation="VREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTN
                     NYRIALRKYQHYQRQTCT"
     gene            323..>704
                     /locus_tag="OQS60_004141"
     CDS             323..>704
                     /locus_tag="OQS60_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS2389734.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTIS"
BASE COUNT          187 a          158 c          177 g          182 t
ORIGIN      
        1 ggtgcgtgaa tatgtcgttc gtctgcgccg tttgggtaat catctacacg agcaaaatat
       61 ttccctcgat cagctgcagg acggtttcct tgatgaaatc ctcgccccgt ggctgcccac
      121 caccagcacc aacaattacc gcattgcgtt acggaagtat caacactatc agcgtcaaac
      181 gtgtacctga cttgtgcaga aatcctccag tctgcctgct tctgatatat attaaataag
      241 aataagatgt agcggagttg tttttgtgtt tacaaacaat ggctctacac tgcaaacaga
      301 cataacaaca ttcggggtga atatgaaatt agcacatctg ggacgtcagg cattgatggg
      361 tgtgatggcc gtggcgctgg ttgcgggtat gagcgttaaa agttttgcag atgaaggtct
      421 gcttaataaa gtcaaagagc gcggcacgct gctggtaggg ctggaaggaa cttatccgcc
      481 gttcagtttt cagggggatg acggcaaatt aaccggtttt gaagtggaat ttgcccaaca
      541 gctggcaaaa catcttggcg ttgaggcgtc actaaaaccg accaaatggg acggtatgct
      601 ggcgtcgctg gactctaaac gcattgatgt ggtgattaac caggtcacca tttctgacga
      661 gcgcaagaaa aaatacgatt tttctacccc ttacaccatt tccg
//
LOCUS       DAJVYM010000360          701 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVYM010000360 DAJVYM010000000
VERSION     DAJVYM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.365"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>701
                     /gene="dcuC"
                     /locus_tag="OQS60_004142"
     CDS             <1..>701
                     /gene="dcuC"
                     /locus_tag="OQS60_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS2389735.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAP"
BASE COUNT          143 a          174 c          181 g          203 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc t
//
LOCUS       DAJVYM010000361          698 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVYM010000361 DAJVYM010000000
VERSION     DAJVYM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.366"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..520
                     /locus_tag="OQS60_004143"
     CDS             <1..520
                     /locus_tag="OQS60_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS2389736.1"
                     /translation="DLVSRTRAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNH
                     MVEAFRRDLKQYHSQLNNITDAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIF
                     VDFQQQGERGLTNAPDEDPDDLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDR
                     FVEAILQFWRER"
BASE COUNT          208 a          152 c          166 g          172 t
ORIGIN      
        1 ggatttagtc agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg
       61 cgtatgctgg atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca
      121 acactttaat catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct
      181 taataatatt actgacgcgc catggttttg cggcgatacc acctggtact ggaaagaaaa
      241 tttcccgcat tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat
      301 tattttcgtc gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga
      361 cccggacgat ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac
      421 gacggcactg cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag
      481 atttgtagaa gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg
      541 ggatgtcctt gttcgttctc agagtagaca acaaaaaaca aataagatca aggtaatatc
      601 tcttcacttg cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg
      661 aaaaacgcgc accgcataaa ccgttgttat tgcttaca
//
LOCUS       DAJVYM010000362          674 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVYM010000362 DAJVYM010000000
VERSION     DAJVYM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.367"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(1..674)
                     /locus_tag="OQS60_004144"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="OQS60_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935603.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          162 a          156 c          186 g          170 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgtt
//
LOCUS       DAJVYM010000363          674 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVYM010000363 DAJVYM010000000
VERSION     DAJVYM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.368"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OQS60_004145"
     CDS             <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OQS60_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS2389737.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTDEVLALRLSENGSQLHHS"
BASE COUNT          163 a          185 c          195 g          131 t
ORIGIN      
        1 accggcgctc tgctctccct gggcagggaa atgttccgcc tcgaaattct ggaggacatt
       61 gcccgggata aagtcagaac tctccatttt gtggatgaga tagaagtcta cctggccttc
      121 cagaccatgc tcgcagagaa acttcagctc tccactgccg tgaaggaaat gcgtttctat
      181 ggcgtgtcgg gagtgacagc aaatgacctc cgcactgccg aagccatggt cagaagccgt
      241 gaagagaatg aatttacgga ctggttctcc ctctggggac catggcatgc tgtactgaag
      301 cgtacggaag ctgaccgctg ggcgctggca gaagagcaga aatatgagat gctggagaat
      361 gagtaccctc agagggtggc tgaccggctg aaagcatcag gtctgagcgg tgatgcggat
      421 gcggagaggg aagccggtgc acaggtgatg cgtgagactg aacagcagat ttaccgtcag
      481 ctgactgacg aggtactggc cctgcgattg tctgaaaacg gctcacaact gcaccattca
      541 taatcacatc gcataaacca cagaccggac tgactccgga aaaacagagg cccgcccccg
      601 ggcctccccg gattcatccg tttccctgtt cagcctgaca gcacgccccc gcggccggat
      661 gacagactcc gctt
//
LOCUS       DAJVYM010000364          674 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVYM010000364 DAJVYM010000000
VERSION     DAJVYM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.369"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>674
                     /locus_tag="OQS60_004146"
     CDS             <1..>674
                     /locus_tag="OQS60_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2389738.1"
                     /translation="GKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQN
                     RAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQ
                     SCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLA
                     RLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLK
                     VSNMSK"
BASE COUNT          201 a          165 c          168 g          140 t
ORIGIN      
        1 cgggaaaatg gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc aatggcttaa
       61 agatgccccg tcacagccat tgcaacagtc actgaaagac cttgagcggg cttacaaaaa
      121 cttcttccag aatcgggctg cttttccccg attcaaaaag cggggacaga atgatgtatt
      181 ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact
      241 gggctggatg cgctaccgga acagccgtca ggtcacgggt gttgtgaaaa atgtcactgt
      301 cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatcaactcc
      361 ggttcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcgccacgct
      421 gtcagatggc acagtctttg agcctgtaaa cagttttcag aaaaaccaga agaagctggc
      481 gagacttcag cgacagttaa gccgcaaggt caaattcagc aacaactggc agaagcagaa
      541 acgcaaaata cagcgactgc attcctgtat cgcaaatatc cgcagggact accttcacaa
      601 agtcacaacg accgtcagca aaaaccacgc aatgatagtc attgaggatt tgaaggtcag
      661 caacatgtca aagt
//
LOCUS       DAJVYM010000365          673 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVYM010000365 DAJVYM010000000
VERSION     DAJVYM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.370"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..560
                     /locus_tag="OQS60_004147"
     CDS             <1..560
                     /locus_tag="OQS60_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS2389739.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          240 a          118 c          164 g          151 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atc
//
LOCUS       DAJVYM010000366          672 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVYM010000366 DAJVYM010000000
VERSION     DAJVYM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.371"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..184
                     /locus_tag="OQS60_004148"
     CDS             <1..184
                     /locus_tag="OQS60_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389740.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>672
                     /locus_tag="OQS60_004149"
     CDS             194..>672
                     /locus_tag="OQS60_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS2389741.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVQDTNNQRNYMFE
                     RLDLGVGETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLV
                     "
BASE COUNT          168 a          156 c          175 g          173 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgcaggaca cgaataatca
      481 acgcaattat atgtttgagc ggcttgatct tggcgttggc gaaacagttt acggtctggg
      541 agagcgcttt actgccctgg tgcgcaatgg ccagacggta gagacctgga accgggacgg
      601 cggcacaagt actgaacagg cgtataaaaa tatcccgttc tacatgacta accgtggtta
      661 tggggtactg gt
//
LOCUS       DAJVYM010000367          669 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVYM010000367 DAJVYM010000000
VERSION     DAJVYM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.372"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(146..>669)
                     /gene="carB"
                     /locus_tag="OQS60_004150"
     CDS             complement(146..>669)
                     /gene="carB"
                     /locus_tag="OQS60_004150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HCS2389742.1"
                     /translation="PGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKHGR
                     ALLSVREGDKERVVDLAAKLLKQGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQ
                     DRIKNGEYTYIINTTSGRRAIEDSRVIRRSALQYKVHYDTTLNGGFATAMALNADATE
                     KVISVQEMHAQIK"
BASE COUNT          149 a          188 c          166 g          166 t
ORIGIN      
        1 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttaattaat
       61 gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcaa attagcggat
      121 gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc tgcaccgaaa
      181 ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
      241 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
      301 tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc ttgatgcggt
      361 cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta atgcccgctt
      421 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
      481 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc aacgcacgac
      541 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg
      601 tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac agcgggtcaa
      661 cacccggga
//
LOCUS       DAJVYM010000368          662 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVYM010000368 DAJVYM010000000
VERSION     DAJVYM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.373"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>662
                     /locus_tag="OQS60_004151"
     CDS             <1..>662
                     /locus_tag="OQS60_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2389743.1"
                     /translation="APDGDIIRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT
                     DCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGD
                     LTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYD
                     VLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAER
                     WQ"
BASE COUNT          164 a          199 c          191 g          108 t
ORIGIN      
        1 gcccctgacg gcgatatcat ccgctaccgt tatgataatc cacacagtga cttaccctgc
       61 gcaacggaag atgccaccgg cagccggaaa accatgacgt ggagccgtta cggtcagttg
      121 ctgagcttca ccgactgttc cggttatgta acccgttatg accatgaccg cttcgggcag
      181 atgacggcgg tgcaccgcga ggaagggctg agtcagtacc gcgcatacga cagccgtgga
      241 cagttaattg ccgtgaaaga cacgcagggc catgaaacgc ggtatgaata caacgccgcc
      301 ggtgacctga ccaccgtcat tgccccggac ggcagcagaa acgggacaca gtacgatgcg
      361 tggggaaaag ctatctgtac cacgcagggc ggtctgacgc gcagtatgga atacgatgct
      421 gccggacggg tcatccgcct gaccagtgaa aacggcagcc acaccacctt ccgttacgat
      481 gtactcgacc tgctgataca ggaaaccggc tttgacggcc gcacacagcg ttatcaccac
      541 gacctgaccg gcaaacttat ccgcagcgag gatgaggggc tggtcaccca ctggcactat
      601 gacgaagcag accgcctcac gcaccgcacc gtgaatggcg aaaccgcaga gcggtggcag
      661 ta
//
LOCUS       DAJVYM010000369          659 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVYM010000369 DAJVYM010000000
VERSION     DAJVYM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.374"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(67..>659)
                     /locus_tag="OQS60_004152"
     CDS             complement(67..>659)
                     /locus_tag="OQS60_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCS2389744.1"
                     /translation="KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETIN
                     KLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHL
                     GDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVG
                     KQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT          132 a          179 c          188 g          160 t
ORIGIN      
        1 aaaaggtggc gtttgtgcag agggactagt gccgggtgcg gcgtaacgcc tgatccggcc
       61 taccgattaa tcgtccagga agctacgcaa cacttcagaa cggctcgggt gacgcagttt
      121 gcgcagcgcc ttcgcttcga tctgacggat acgttcgcgg gtaacgtcga actgtttacc
      181 cacttcttcc agcgtgtggt cggtgttcat atcgataccg aaacgcatac gcagaacttt
      241 cgcttcacgc gcggtcaggc cagccagcac gtcgtgcgtt gccgcacgca ggctttcggt
      301 ggtcgcagaa tccagcggca gctcgagggt ggtatcctcg atgaaatccc ccagatgcga
      361 atcttcatca tcaccgatcg gcgtttccat ggagattggc tctttggcga tcttcagcac
      421 tttgcggatc ttgtcttccg gcatcagcat acgttcagcc agttcttccg gcgtcggttc
      481 acggcccatc tcttgcagca tctggcgaga aatacggttg agtttgttga tagtctcaat
      541 catatgcacc ggaatacgga tggtgcgcgc ctgatccgcg atagaacggg tgatcgcctg
      601 acggatccac caggttgcgt aggtggagaa cttgtaacca cggcggtatt cgaatttat
//
LOCUS       DAJVYM010000370          644 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVYM010000370 DAJVYM010000000
VERSION     DAJVYM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.375"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            123..629
                     /gene="mug"
                     /locus_tag="OQS60_004153"
     CDS             123..629
                     /gene="mug"
                     /locus_tag="OQS60_004153"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS2389745.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          161 a          143 c          181 g          159 t
ORIGIN      
        1 ggtagcggat tataaagtaa ctccgtgtcg caatccagcc attaccgtgc gcctgctgtt
       61 tttataaaca atgtattttc aatccgttat acttttcctg gtgatgttag aaaggagtaa
      121 atatggttga ggatattttg gctccagggt tacgggtcgt attttgcggt atcaaccctg
      181 ggctttcatc cgccgggact ggttttccct ttgctcatcc agcaaatcgc ttctggaagg
      241 tgatatatca ggccgggttt accgaccgtc agttgaagcc gcaggaggca cagcatctgc
      301 tggattatcg ttgtggcgtc accaaactgg tagaccgtcc aacggtgcaa gccaatgaag
      361 tttcaaagca ggagctgcac gcaggtgggc gtaagctgat tgaaaaaatt gaagattatc
      421 agccgcaggc gttggcgatt ctgggcaaac aagcatatga acagggattc agccagcgcg
      481 gtgcacagtg ggggaaacaa acgctcacca ttggttcgac gcagatttgg gtgctgccaa
      541 atcccagcgg tttaagtcgc gtttcactag agaaactggt tgaagcgtat cgcgagctgg
      601 accaggcgct ggtagtgcgt gggcgataaa aaacgccacc gaaa
//
LOCUS       DAJVYM010000371          637 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVYM010000371 DAJVYM010000000
VERSION     DAJVYM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.376"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(518..>637)
                     /locus_tag="OQS60_004154"
     CDS             complement(518..>637)
                     /locus_tag="OQS60_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2389746.1"
                     /translation="RPVKASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          198 a          130 c           97 g          212 t
ORIGIN      
        1 aatcgtggta acagcgtgtc atgctcagtt gcagcaccat ctagactgaa aataaattca
       61 cggtgtaagt tcttaaataa caaaaatgga gttctccagt aatcagaacc cccatgatgc
      121 ttcccgatta aaatatgctc aattgacata agaaataaaa atttttattg caatcactct
      181 tgacaaggtt tactctcttt taagagatgc ttaacaggtt ttctaaacgg atatcctttc
      241 tgggatatct atcttttatc ttaaaagttt ttacagatac tttaaagata tctgtatata
      301 ttctgtacag taacttttca gagtctgaaa atttttataa aaaatgacta gccctctttg
      361 aatctcatct tccccgaatc ttcgaaaata tctgtctagt tgattcggta gaatctacct
      421 catgatgtat acgtgtttag tacccgtcac accagctatg tgaccccctt ggcaaaaaag
      481 ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt ctgtaataag
      541 ttgctctaga gattcctagc tcttcctgaa tctgcaacct cgttttacct tgtgaaataa
      601 gcatctgtac agcctctgaa gaagctttta caggtct
//
LOCUS       DAJVYM010000372          632 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVYM010000372 DAJVYM010000000
VERSION     DAJVYM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.377"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(163..>632)
                     /locus_tag="OQS60_004155"
     CDS             complement(163..>632)
                     /locus_tag="OQS60_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS2389747.1"
                     /translation="NQWTTAAGKTGLKLKGLGKISLIGGLLELGMMAQEFEKAHPWLV
                     KNFVADALNSGFGLNDKFDEWGKQFHDFVYDMTGWQMPRGDGYLSPDKRYTPNVSLER
                     NQLLSLASSPATRSELKVTFDNAPPGMRVIDLPKTGDPFMKITHDVGYSPFKR"
BASE COUNT          173 a          161 c          118 g          180 t
ORIGIN      
        1 ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca gcaagaggtt
       61 aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt gttagcatgt
      121 tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt taaatggaga
      181 atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca aatcgataac
      241 gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg ttgccggtga
      301 agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt agcgtttatc
      361 gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat agacaaaatc
      421 atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac tgttcagtgc
      481 atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct gcgccatcat
      541 gcccagttca agcaatccac caatcagact gatctttccg aggcccttta atttcagccc
      601 tgtttttcct gccgccgttg tccattgatt ct
//
LOCUS       DAJVYM010000373          623 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVYM010000373 DAJVYM010000000
VERSION     DAJVYM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.378"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(95..442)
                     /gene="tnpB"
                     /locus_tag="OQS60_004156"
     CDS             complement(95..442)
                     /gene="tnpB"
                     /locus_tag="OQS60_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS2389748.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          134 a          169 c          179 g          141 t
ORIGIN      
        1 gcgctgtttc agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac
       61 cgggcttatt ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca
      121 gccgcttagg ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgtgtaa
      181 ggaacacttt gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg
      241 tcaggaggca cagtccgtca ctggtggacc acagcagttt aacctgactg ccgctgcggc
      301 cccggaaaat gaaaacatgg ccggacatgg gatcgtcttt cagcgccgtc tgtactttcg
      361 cagccaggcc gttgaagcca tttctcatat cggtgatacc ggcaaccagc caaattttga
      421 tcccggaagg taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc
      481 gctgacattg ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact
      541 gagggttttc gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac
      601 aggaagtagc tgggggctct ctg
//
LOCUS       DAJVYM010000374          623 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVYM010000374 DAJVYM010000000
VERSION     DAJVYM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.379"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>623)
                     /locus_tag="OQS60_004157"
     CDS             complement(<1..>623)
                     /locus_tag="OQS60_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2389749.1"
                     /translation="LPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDRQGRLAEETSR
                     NGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTDCSGYTTRYEYDRYGQ
                     QIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTAIVAPDGSRSEIQY
                     DAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLT"
BASE COUNT          105 a          205 c          168 g          145 t
ORIGIN      
        1 gtcagcctgt cgaccgggtc ataccggaac gtggactggc tgccgttctc gttggtcagc
       61 acagtgatgc gcccggcggc gtcatacccc atgcttcgcg tcagaccgcc ctgcgtggtg
      121 ctgacggcct ttccccacgc atcatactgt atctcactgc ggctgccgtc cggggcaacg
      181 atagcggtga ggtctcctgc ggcgctgtac tcataacggg tttcacgccc ctgcgcatcc
      241 ctctgactga ccagttgacc gcgcgggtta taactgctgt aagtgctgat gccttcttcc
      301 cggtgaacgg cgatttgttg accgtaccgg tcatactcat accgcgtcgt gtaccccgag
      361 cagtccgtaa acgcctgaag ctgaccgtac cggtttcgcg ttattctccg cctcaccccg
      421 gttccgtctt ccaccgcaca gggcaggcca ctgcgggaag aatcataaaa ccagcgcgta
      481 atgttaccgt tgcgcgaggt ctcttccgcc agcctgccct gacggtcata tttccggctg
      541 ctgcgtaaac catccggata tgtcactgac gtcagttgca actggttgtt gtaaccatac
      601 cgtaccgtcc tgccgtccgg cag
//
LOCUS       DAJVYM010000375          610 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVYM010000375 DAJVYM010000000
VERSION     DAJVYM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.380"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..134)
                     /locus_tag="OQS60_004158"
     CDS             complement(<1..134)
                     /locus_tag="OQS60_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HCS2389750.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     T"
     gene            complement(152..>610)
                     /locus_tag="OQS60_004159"
     CDS             complement(152..>610)
                     /locus_tag="OQS60_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2389751.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
BASE COUNT          148 a          145 c          137 g          180 t
ORIGIN      
        1 gttggatagg gtttgtggat ttttgctggc tgacgggcaa ataaagtttg tttaatggtt
       61 tgtttatctg ccagttgatt aatcacatgg cgggctatct gaccgccagc accaagaatc
      121 agtacatttt tcataatatt tctcatgaaa cttatcccag atgggtgttg aaaaaaggaa
      181 taactttttc cagcgcctcc tgaactgctt ccggtttgtc atacagttca taatgtgaga
      241 aatcgggtaa tgaaactaac tgacgatcct gcgaaaccat cgcccggcca tagacttcca
      301 gtccatcacg ataagcaccg aaggcaccga ctttttcacc aactactacc atcaccggct
      361 gagtcagtaa cacttcagta aatgcgaagg catcccaggc cagcgttttc tgtgcatgtg
      421 agaacaacat ttttgtcgca cctcccggtt gttgcccgcg tggtgtttta tagtagtcag
      481 ttgcttcata aacatcgcgt tcggtcagtc catgggcttt tgctgcatcc aggctggcgg
      541 gaagtaattc gttgatctgt aattctccac cacgagcctc tttggttcgt tgcgacgcca
      601 ttgcattcaa
//
LOCUS       DAJVYM010000376          607 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVYM010000376 DAJVYM010000000
VERSION     DAJVYM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.381"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..209)
                     /locus_tag="OQS60_004160"
     CDS             complement(<1..209)
                     /locus_tag="OQS60_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS2389752.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEA"
     gene            complement(209..424)
                     /gene="essD"
                     /locus_tag="OQS60_004161"
     CDS             complement(209..424)
                     /gene="essD"
                     /locus_tag="OQS60_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCS2389753.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          171 a          158 c          127 g          151 t
ORIGIN      
        1 tctgcttcgg tatacgtttt accgggaatg atgtcttttc ctgtatgccc gtaacataca
       61 gtccatacac caactatgtc tttgtaagga ttatgtctca caccttccag accatcgtta
      121 ccacctgggc cagtgattaa cacagatgct atagcaatag ccccgccacc aatagcagca
      181 gcaacagctt ttcgtaatga tggaggcatt atccacctct cgcagccttg cgcttatctt
      241 ctttaatctt gaaataaagg tttgtcaggt acgtcagcag gccaaatacc aggctaccca
      301 gcactccaat tgccgcccac tgtgagggcg tgactttatc tagcagctgt aaaaaccagt
      361 acccggcact acctgctgag gtgccatagg cgacacccgt tgttaactta tccatggatt
      421 tcataacccc acctcgcaga caaagcgggt gtaaattgag ggaatactac gaaacgtaac
      481 agactcggag tcagtgaata actcaggtat tgggttatca gctaatatcg agactcaaaa
      541 aatggaaaaa cccgctcgac ggcgggttta agctgtgtga cgaagtaacc actcttaaca
      601 gcataac
//
LOCUS       DAJVYM010000377          604 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVYM010000377 DAJVYM010000000
VERSION     DAJVYM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.382"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>604)
                     /locus_tag="OQS60_004162"
     CDS             complement(<1..>604)
                     /locus_tag="OQS60_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389754.1"
                     /translation="PLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDIRLQIRDEGLI
                     LNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQVLPEDVRLSP
                     HVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDG
                     AGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQV"
BASE COUNT          115 a          206 c          189 g           94 t
ORIGIN      
        1 acctgagaag aggaaacaga acggggactg gccggggaag ataaggaaga ggcgcgctgt
       61 ttacgggacg cttccgcccg ctgcgcctgc gtgctcagcg ccatgtgaaa acggcggccc
      121 gcgccgtccg tcacccccgt caccgcgccc gccacgtcac cctccgccgc ccggtggaaa
      181 gtcagtgagc gcccgaagcc atccaccacg accgtcagta cccggtatgc gggcggctcc
      241 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      301 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc ttagcggctg cgaactgtgc
      361 tgcgccgcca ctccgccccg cgccagccag agtgactcgc tgcggctgta gcttatctcg
      421 ccgggaaaca gcggctcaaa gtgaatgctc ctgccgccgc tgtcgttgag tatcaggcct
      481 tcatcgcgta tctgtaagcg gatatcgaag ggcgctttcc agccgggacc aaacaccccc
      541 accggcgcgg gtgttctggt ccggtagctg ctgtaggcgc ggaaaagaat aaacggcagc
      601 gggc
//
LOCUS       DAJVYM010000378          597 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVYM010000378 DAJVYM010000000
VERSION     DAJVYM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.383"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..500
                     /gene="proC"
                     /locus_tag="OQS60_004163"
     CDS             <1..500
                     /gene="proC"
                     /locus_tag="OQS60_004163"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS2389755.1"
                     /translation="LARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVL
                     NIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQ
                     AVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSE
                     KLSKS"
BASE COUNT          135 a          163 c          174 g          125 t
ORIGIN      
        1 agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg aacactcccg
       61 cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc cccgaagata
      121 ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt gctgagccga
      181 tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt atgtttatcg
      241 aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg tataaatttg
      301 ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa catccggggg
      361 cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta cgcgtactgg
      421 aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag
      481 aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt cggtgcggtt
      541 acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc actcacg
//
LOCUS       DAJVYM010000379          596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVYM010000379 DAJVYM010000000
VERSION     DAJVYM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.384"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            20..430
                     /locus_tag="OQS60_004164"
     CDS             20..430
                     /locus_tag="OQS60_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCS2389756.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          198 a          118 c          109 g          171 t
ORIGIN      
        1 tcacatggaa ggtactacaa tggctcaggt tgccattttt aaacaaatat tcgataaagt
       61 gcgaaataat ttaaactatc actggtttta ttctgaacta aaacgtcaca atgtctcaca
      121 ttacatttac tatttagcca cagagaatat tcatcttgtt cttgaaaacg ataatacggt
      181 tttaataaaa ggacagggta aggttgtaaa tgtaagattt tcaaaaaata aatgccttat
      241 agaagccacc ttaaaaggat tcaaatcagg agagttatca ttttacgaat acaggaaaaa
      301 tcttgctaca gcaggggttt tcagatggat tacaaatatc cacgaaaaca aaaggtatta
      361 ctataccttt gataattcat tactctttac tgagaacata cagaacacta cacaaatatt
      421 tccgcactaa atcataacgt ccggtttctt ccgtgccaga accggactcg ctggcatgat
      481 gaaatatgtg tacccggtaa ccccggtgtg catcgttttt gattattccc gcacactcgc
      541 gcagaaggag ttccccgtcg ggctacggtc tctgttaata cgggaatacg gcgacg
//
LOCUS       DAJVYM010000380          589 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVYM010000380 DAJVYM010000000
VERSION     DAJVYM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.385"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>589
                     /locus_tag="OQS60_004165"
     CDS             <1..>589
                     /locus_tag="OQS60_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCS2389757.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     IMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          139 a          140 c          186 g          124 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagt
//
LOCUS       DAJVYM010000381          588 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVYM010000381 DAJVYM010000000
VERSION     DAJVYM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.386"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..588
                     /locus_tag="OQS60_004166"
                     /pseudo
     CDS             1..588
                     /locus_tag="OQS60_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    98..214
                     /locus_tag="OQS60_004166"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          136 a          144 c          182 g          126 t
ORIGIN      
        1 atggagggct caccaccgct gaacgtcagc gtctgaaaga gctggagcgt gagaatcgtg
       61 aactgcgctg cagtaacgat atccttcgcc aggcttccgc ttattttgcg aaggcggagt
      121 tcgaccgcct ctggaaaaaa taatgccact gctggataag ctgcgtgggc agtacggggt
      181 cggaccggta tgcagtgaac tgcatattgc cccgtcaacg tattaccact gtcagcaaca
      241 ggacatcatc ctgataaacg cagtgcccgt gcgcagcacg atgactggct gaagagagag
      301 atacagcgcg tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgccag
      361 ttgttacggg aaggaatcag ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg
      421 ggacttgtcg gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg gaaaaccgtt
      481 gccgcaggtg accgcgtaaa ccgtcagttc gtggcagaac gtcctgacca gctgtgggtg
      541 gctgatttta cttacgtcag cacatggcag ggcttcgtct atgtggcg
//
LOCUS       DAJVYM010000382          586 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVYM010000382 DAJVYM010000000
VERSION     DAJVYM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.387"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>586)
                     /locus_tag="OQS60_004167"
     CDS             complement(<1..>586)
                     /locus_tag="OQS60_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCS2389758.1"
                     /translation="SIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDG
                     ILIIVAWSDRTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALN
                     NQLTSQHQYPTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVC
                     ISAIYLLDLFLFSDKIFSIAVFSFFFTFTIFMVFT"
BASE COUNT          201 a          104 c          117 g          164 t
ORIGIN      
        1 gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat ggaaaaaatt
       61 ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac tccactttta
      121 catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa catcactgca
      181 aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc actttctgaa
      241 ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt tatagctagc
      301 tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct acggatgata
      361 tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg gatgcggaca
      421 gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc atctcctaaa
      481 cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt ggttgtggga
      541 acaattaata cagcaagttg cacttgggta ttattgaaaa tagatt
//
LOCUS       DAJVYM010000383          585 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVYM010000383 DAJVYM010000000
VERSION     DAJVYM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.388"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..218)
                     /locus_tag="OQS60_004168"
     CDS             complement(<1..218)
                     /locus_tag="OQS60_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCS2389759.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWL"
     gene            complement(211..555)
                     /locus_tag="OQS60_004169"
     CDS             complement(211..555)
                     /locus_tag="OQS60_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS2389760.1"
                     /translation="MIISLFTWRRAGRDDAPRIFGWWGDTWPAVQNDRTGSRLYLLRR
                     SKLTNKTPQLARDYVREALAWMVEDGVASRLDINAVRTGTDSLALAITIYQRDGNIHN
                     IIFDDIWSELNG"
BASE COUNT          161 a          170 c          135 g          119 t
ORIGIN      
        1 tacagccagt cttcatcaca cacgtccggt aacagatttc tggcaagata atcgatatac
       61 ccgtacaacg tgtgtacggc tgcagcctgt acacgggcat acacttccgc atccatacga
      121 cgaagcagcg tatcctgctc aaagcgggtt aataaatcgc tccggatcat agaaataagt
      181 tgcgggaggc cggggcgata aaactgactg tcagccattc agttcactcc agatatcatc
      241 aaaaataatg ttgtgaatat tgccgtcacg ctggtaaatg gtaatggcaa gtgccagcga
      301 gtctgtcccg gtccggacag cgttaatatc aagacgggaa gcaacaccat cctccaccat
      361 ccacgccagc gcctcacgga cataatctct ggcaagctgc ggggttttat ttgtcagctt
      421 gctgcgtcgc aacagataca aacgcgaccc cgtgcgatca ttctgaaccg caggccaggt
      481 atccccccac catccaaata tccgtggtgc atcatcacgc ccggcacgcc gccatgtaaa
      541 aagcgaaata atcacagcac gcgtcagaag gtcgatcgaa gcccc
//
LOCUS       DAJVYM010000384          578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVYM010000384 DAJVYM010000000
VERSION     DAJVYM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.389"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          173 a          139 c          119 g          147 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcctg atctctgtcc cggtatttta gcccgaggta
      301 tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca ttcgctaaca
      361 gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg ttcataagcg
      421 tattatcagg attgtctgga aaattcccga tactttggac tattactcct gaacaattcg
      481 aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag gtaagcgtaa
      541 accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAJVYM010000385          578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVYM010000385 DAJVYM010000000
VERSION     DAJVYM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.390"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..408)
                     /gene="rtcR"
                     /locus_tag="OQS60_004170"
     CDS             complement(<1..408)
                     /gene="rtcR"
                     /locus_tag="OQS60_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS2389761.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARL"
BASE COUNT          162 a          139 c          133 g          144 t
ORIGIN      
        1 cagccgggcg ggcaggtaac gcgcttccgc cagcagaaac cagcaaatct gcgcgacgtg
       61 ggtgccggtg gtgatgtgaa ttaaatagtc ttctttttct ggctgaaact cgtaaccacg
      121 ggcgaaatca tgcaggcagg cgtaaacctc ttcgaaatcc cacgggttat gcagttcaat
      181 ctcaacgccc acgacttctg tttctggcga aacgctggcg atatcgcgtt taagcgtttc
      241 gaatagcgag cgcgagcgag tgtcgtgcaa caattccagc cgatcgatga ctaacgattc
      301 ttgctggcat aaacaaagcg tcggacgcca tttcgaccag cgctgactgc cgcgtccggc
      361 ataatccagc acggtaccga caaagccaaa agccactgtt ttacgcatct tagatatcct
      421 tataaaagac gatatttcat tatcggtgaa ttggttttgt ggaaaatctc cgtttacgga
      481 cgtaaagatt taacctattt aaaaacaatg aattaaaaaa ttaatctgct tatttaattt
      541 tctggcacga cggttgcaat tatcacgaca gcaaacaa
//
LOCUS       DAJVYM010000386          578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVYM010000386 DAJVYM010000000
VERSION     DAJVYM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.391"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          157 a          113 c           92 g          216 t
ORIGIN      
        1 tgttatttat acttatcggg tatcagtttt gataggggta tcagacttga taggggtaac
       61 agaattctgt gggggtagtt cactatgacc tacccctagt tcactgtgag ctaccgttac
      121 atccaatcat cttccgatgt aacgtatcgt tactaccgat gtgtaatatc gttacattca
      181 ataattagtc tatgtaatat atcgttacta ccaactttct tacagagagc atttcagata
      241 aatgaatgta acctctcgtt acatccattt gtcacatagc actctgtgta atgtctttct
      301 atgtgtgtgc cataatcccc ctatattccc tgtgtaaatc atagaataat gctctctatt
      361 ccctgtgtaa ttccctacta aaaaatccaa tgaaaagtag cttaaaagtt caattcttac
      421 tttcttcact tttacatcac tttcgcaaag ccttgtgtaa tgcggcttac agagcaccat
      481 cagaaattgt tggctaaaaa ttgttctgtt tgtttctgaa cgagtttctc gtttgtgcaa
      541 attttaaaat ttattttgtt tttatagcct tatttcgt
//
LOCUS       DAJVYM010000387          571 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVYM010000387 DAJVYM010000000
VERSION     DAJVYM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.392"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..67
                     /locus_tag="OQS60_004171"
     CDS             <1..67
                     /locus_tag="OQS60_004171"
                     /inference="COORDINATES: protein motif:HMM:NF018812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS2389762.1"
                     /translation="TKSKTFKTESGARCWLARNTD"
     gene            67..444
                     /locus_tag="OQS60_004172"
     CDS             67..444
                     /locus_tag="OQS60_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389763.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT          164 a          125 c          133 g          149 t
ORIGIN      
        1 taccaaaagc aaaacgttta aaaccgaatc tggcgcgcgt tgctggttag ccagaaacac
       61 tgactgatga ggctgacgat ggaatttaaa gatttaccgt ctgatgtaca gaaaacagca
      121 gctcatacat tgcattctgt gctgcgagaa atcgggaaag atattgcaag cgagccagca
      181 aaagatctgg cccggaaaat caagaccgct ttcgttgagc tttataatgt tggcactgac
      241 tctgaaactg tcgagaccaa gaccgtaagt tcaccaatat tctcacttga gccaagcgta
      301 ttatcaggtg aaatatgcac cgagatttcc agcgaactgc ttccggtaat acgagaagca
      361 attcgccgtc gcggtttgga tggaagttat gatcatgacg ttctgcaagt tctcaggaca
      421 atggtgactt cactggggat ttgatccctg cgtcctctaa gcttttgata tagcggtcct
      481 ccaagaagcg gtaaattttg tcgaaatcat cttttccctc tggtttattc agaggattac
      541 aggacccgta agtagcggca tacatttcaa c
//
LOCUS       DAJVYM010000388          570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVYM010000388 DAJVYM010000000
VERSION     DAJVYM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.393"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            166..231
                     /locus_tag="OQS60_004173"
                     /pseudo
     CDS             166..231
                     /locus_tag="OQS60_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            459..>570
                     /locus_tag="OQS60_004174"
     CDS             459..>570
                     /locus_tag="OQS60_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS2389764.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDD"
BASE COUNT          187 a          120 c          125 g          138 t
ORIGIN      
        1 ttctttgaac tcttgcagaa aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg
       61 caaaaataaa atcaccctat agatgcacaa aaaacgggca aaactacctg gttcgcaaaa
      121 ctgcgtctaa agttaaaccg ggacctcgcg agcaagggtg agacgatggc gctttacaca
      181 attggtgaag tggcgttgct ttgtgatatt aaccctgtca cgttacgcgc gtgacgcaac
      241 atttttaaaa atcgacgaag atcgtccagg agcgccgttt ttcaagggtt ggatagatga
      301 ttcgtttacc atgtcgacga acttcgacga atttcgccga taaaaagtgt cccatacatg
      361 ccccattgat gccccataaa caatatcagg aattgacact ggttatccat acagataaaa
      421 ataacacctg tacataacca cagttacagg agggtgttat gcgtgttgag atctgtatcg
      481 ctaaagaaaa aatcactaaa atgccaaacg gtgctgtgga tgcgttaaag gaagaattaa
      541 cccgacgcat cagtaaacgt tatgacgatg
//
LOCUS       DAJVYM010000389          559 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVYM010000389 DAJVYM010000000
VERSION     DAJVYM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.394"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>559
                     /locus_tag="OQS60_004175"
     CDS             <1..>559
                     /locus_tag="OQS60_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS2389765.1"
                     /translation="NHVQIRQNQPHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNP
                     VNGHAHLLYALNIAVRTAPDSSVKALKYAAAIERSLCEKLCADVNYSGLICKNPFHLE
                     WQVMEWREEAYTLDELADYLDLSASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGW
                     PEFSQWLDAVIQRVEMYNASLPVPLS"
BASE COUNT          128 a          147 c          159 g          125 t
ORIGIN      
        1 gaaatcatgt ccagatccgg cagaaccagc ctcatgcgca gttctggctg gtttttgacg
       61 ttgatcgcga gggggcagcg attgactgga gcgaccggaa cgctcccgcg ccgaacatca
      121 ccgtaaaaaa tcctgtgaac ggtcatgctc acctgctcta cgcgctcaat atcgccgtga
      181 gaaccgcgcc ggacagctcg gttaaggcgc tgaaatacgc cgctgcgatt gaacgttcgc
      241 tgtgtgagaa actttgtgct gatgtgaact acagcggact gatttgtaaa aatccgttcc
      301 acctggaatg gcaggtgatg gagtggcgag aggaagccta taccctcgat gaactggctg
      361 attatctcga tttgagcgcc tcagcgcgcc gtagcatcga taaacattac gggatggggc
      421 gaaactgcca cctgttcgaa atgacgcgca aatgggctta cagggcgatt cgtcagggct
      481 ggcccgaatt ctcacagtgg cttgatgccg tgattcagcg tgtcgaaatg tacaacgcat
      541 cgcttcccgt tccgctttc
//
LOCUS       DAJVYM010000390          555 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVYM010000390 DAJVYM010000000
VERSION     DAJVYM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.395"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OQS60_004176"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OQS60_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS2389766.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..>555)
                     /locus_tag="OQS60_004177"
     CDS             complement(390..>555)
                     /locus_tag="OQS60_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2389767.1"
                     /translation="IGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPVLN
                     NRRFAPPQLL"
BASE COUNT          147 a          154 c          124 g          130 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgata
//
LOCUS       DAJVYM010000391          554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVYM010000391 DAJVYM010000000
VERSION     DAJVYM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.396"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          177 a          115 c           92 g          170 t
ORIGIN      
        1 acgttcaaac atctgtttag aaatgccaaa ttgttgatct aacaaataaa cctgtataaa
       61 cagaacacca tttaatctat tcccctgtgt aacccaccac atcacacaat aaaaacaaaa
      121 aactttaatt atatttcata gcacacaaaa gtcaatagaa gccctttcgt gctcctacgt
      181 aaaaaatcca tcacttttcc actgatacat caattcaagg cgttatatgt gttccttagc
      241 acatataaca tttattatgt taaatggatt ttggcctata ttttgacctg cacccgcata
      301 aacactggtt tcatcgctta cagcccttgt gagacgtggt gctgtttcta gcatctgatc
      361 cgacataaaa ccgatcattc tcgtgtttag tgaaagttta gtgagagaca ttacagaaag
      421 gctttctgtg taatgattta ataggtaaaa acgatgttga ttacgcaaga aaattccttg
      481 ctgatatttg gggattctgg attttccaga tcggggggcg ctgaaaatcg cgttaagcac
      541 ctctcgcaat ctag
//
LOCUS       DAJVYM010000392          554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVYM010000392 DAJVYM010000000
VERSION     DAJVYM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.397"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>554
                     /locus_tag="OQS60_004178"
     CDS             <1..>554
                     /locus_tag="OQS60_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2389768.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGN"
BASE COUNT          153 a          119 c          140 g          142 t
ORIGIN      
        1 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
       61 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
      121 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
      181 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
      241 gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa tactggctat
      301 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgaatca
      361 ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg tcaagtgtct
      421 ctcagcaccg acggtggttt tgtattgcat agcggcggcc tgaccttcag taatgacagt
      481 tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg agcgcgaata
      541 aattatggca acag
//
LOCUS       DAJVYM010000393          547 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVYM010000393 DAJVYM010000000
VERSION     DAJVYM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.398"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..547
                     /locus_tag="OQS60_004179"
                     /pseudo
     CDS             1..547
                     /locus_tag="OQS60_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          194 a          120 c           83 g          150 t
ORIGIN      
        1 tccgacttat taaaaatgcc tgtttaaaat tatataattt agcacatacc gcaaccaact
       61 cccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa aagctattct
      121 ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct gaaaaacagt
      181 cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatggt acaaacttct
      241 gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag aagatcgccg
      301 ctaatgcaaa aaatactcaa gtcaattatc ctactggtct ctacgtcccc tactccacca
      361 gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca tggccgaatg
      421 aagtccagtt attccccata aatactggcg gacactggat attagtttcg ctacagaaaa
      481 tagtaaataa aaaaataata aactacaaat aaaatgcgtc atattcaact cattgcgtgc
      541 actaggc
//
LOCUS       DAJVYM010000394          543 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVYM010000394 DAJVYM010000000
VERSION     DAJVYM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.399"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..83
                     /locus_tag="OQS60_004180"
     CDS             <1..83
                     /locus_tag="OQS60_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS2389769.1"
                     /translation="PEGGDLIALAPGESTTSEMSLRVEWL"
     gene            complement(131..>543)
                     /locus_tag="OQS60_004181"
     CDS             complement(131..>543)
                     /locus_tag="OQS60_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS2389770.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT          166 a          145 c          130 g          102 t
ORIGIN      
        1 gcccggaagg gggtgatctc attgcgctag caccagggga atcaacaact tcagagatgt
       61 cgttgcgggt agagtggctg taatttatta aatgccggat aaggcgttca cgccgcatcc
      121 ggcatctttc ctaacgccga tcgagagaaa ttgcccccgg cccggtaatt gccaatagca
      181 agaacgcgcc agcgatactg acattcttcc agaaattaat catatttggc ccaaccgcat
      241 cgccggtcat atcccagtaa tggtgaccaa tcaccgccgt acccagcgtg tagaaaataa
      301 acagcaccgc cagcggacgg gtgaaaaagc caagcacgat taatatcgcg gcgggcactt
      361 ccataactac cgcaataatc gctgccagca tcggcattgg cgcgcccaac gaggccatat
      421 attggaccgt accgtcaaag cccatcattt tgggaaaacc aaaaataatg aaaattaaga
      481 ccacggcgat acgggcaatt aataacaaaa cggggcgggc agctccaaaa tcaaaataac
      541 gta
//
LOCUS       DAJVYM010000395          541 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVYM010000395 DAJVYM010000000
VERSION     DAJVYM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.400"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>541
                     /gene="gspA"
                     /locus_tag="OQS60_004182"
     CDS             <1..>541
                     /gene="gspA"
                     /locus_tag="OQS60_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCS2389771.1"
                     /translation="RVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLL
                     HALHRTDSRCPLIIIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQ
                     DIELVSTGRLTEHELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETI
                     RLLQQAEQTSVFTAKQWRMI"
BASE COUNT          142 a          134 c          132 g          133 t
ORIGIN      
        1 ccgtgtgatt actttaagcc tcgttgtttc ctggcaaggt aaggccgcat ggatcgttac
       61 cgacgataac gcggctgaac agggctgccg tgacagcgcc tggacgcgag atgagatggc
      121 ggggcaatta ctgcatgcgc ttcatcgaac tgatagtcgc tgccctctta ttattattga
      181 aaatgctcac ctgaatcacc gccgcatact tgatgatctc caacgcgcga tatctcttat
      241 tcctgacggt caatttttgc tgatcggcag acccgatcga aaagtcgaac gtgattttaa
      301 aaaacaggac attgaacttg tctcgacagg acgtctgacg gagcacgaac ttaaggcaag
      361 catccttgaa ggtcagaata ttgaccaacc cgatctcctt ttaacctcca gagttctgaa
      421 acggatagct ttattatgtc ggggcgatcg cagaaagctg gcgcttgccg gcgaaacaat
      481 tcgcttatta cagcaagccg agcagaccag cgttttcact gcaaaacaat ggcgaatgat
      541 t
//
LOCUS       DAJVYM010000396          538 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVYM010000396 DAJVYM010000000
VERSION     DAJVYM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.401"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            192..425
                     /locus_tag="OQS60_004183"
     CDS             192..425
                     /locus_tag="OQS60_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS2389772.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(458..>538)
                     /locus_tag="OQS60_004184"
     CDS             complement(458..>538)
                     /locus_tag="OQS60_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="receptor for colicin S4; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS2389773.1"
                     /translation="KSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT          166 a          114 c          129 g          129 t
ORIGIN      
        1 actgcacaat aaaattaatc cgtataagga aaactgatat ggcaactttg acaaaaaaag
       61 aacgggcatg gttgaacgaa ttacaggaag ttcttgatcg ctgtcctgtg gtgtcatcct
      121 gccgcgattg cgttcttagt ccgtggctat tgcaccacca tcacgcgaaa gggacagcta
      181 agcgcggcgg gatggttaag ccagcaacat taaccgttgc atttaaaaaa gcccgggatt
      241 ctgtggatta caactggcgt gctaatggca ccccaccctc tttccatgag cagagatctt
      301 tatcagagcg attgttcaga gagcaggggg ttgataccaa aattttgcta ggccattcga
      361 atcaaaaaat gaccgatatt tacaacgatg tacgcggtaa ggagtggaaa aaactggtca
      421 tttgataacc agttttgcag aggttttgca gagaaaatta aaagcggtac ccggcagaga
      481 acataaacac ccacggatcc agtcgtaccg agtcttttac ggtagtaaca ccagattt
//
LOCUS       DAJVYM010000397          532 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVYM010000397 DAJVYM010000000
VERSION     DAJVYM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.402"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            87..>532
                     /gene="rlmB"
                     /locus_tag="OQS60_004185"
     CDS             87..>532
                     /gene="rlmB"
                     /locus_tag="OQS60_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS2389774.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESV"
BASE COUNT          136 a          143 c          144 g          109 t
ORIGIN      
        1 aatcacgcaa attcaatata ttgcagagat catgtaggcc ggataaacgt agtgcatcag
       61 gcaaaacgta aacaacgagt acattaatga gcgaaatgat ttacggcatc cacgcagtgc
      121 aggccctgct ggagcgcgcc cctgaacgtt ttcaggaagt ctttatttta aaaggccgtg
      181 aagataaacg tctgttaccg ctgattcacg cccttgagtc tcagggcgtg gttatccagt
      241 tggcaaaccg ccaatatctc gacgagaaaa gcgacggtgc cgtgcatcag ggcattatcg
      301 cccgcgtgaa gccaggacgt cagtatcagg aaaacgatct gccggatctg atcgcttcgc
      361 tcgatcaacc gttcctgctg atcctcgacg gtgtaaccga tccgcacaac ctcggcgcgt
      421 gcctgcgtag cgcggacgct gctggcgttc acgcggtgat tgtgccgaaa gatcgctccg
      481 cacagctcaa cgctacggcg aaaaaagtag cctgcggcgc ggcagaaagc gt
//
LOCUS       DAJVYM010000398          525 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVYM010000398 DAJVYM010000000
VERSION     DAJVYM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.403"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            268..369
                     /locus_tag="OQS60_004186"
                     /pseudo
     CDS             268..369
                     /locus_tag="OQS60_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          137 a           91 c          132 g          165 t
ORIGIN      
        1 tttccggaga acgtggtgac ggcagttgaa accaatgtgt tgaggttgca tggacggtta
       61 agccgagaat acggtagtaa gtgagatatg gggtttggtc gttgttaatg agcaatcatg
      121 accggccaac gactggattc tcccttggtt tatgtttgtg cgcataatgt atattatgtt
      181 aaatcatgtc tgagtgatgc gaagtgcttt tctggtagtc gttattcgtt cagattaggt
      241 gtccttgtga tttaaagatg ttatcgtggc acgaatatac tgccattcca ggctcgttat
      301 attgctctcg ggcagtccgt agctggtact gcaaacgctg atgcgacctt caaagttcaa
      361 tatctataat tcaatcttaa ggtggctata cgtcatctga aattgaaaca gggtgtgtgt
      421 ggcatggcta cgcacacccg agcaattcac caatgtgaat ctcattatta atgaggagtg
      481 gtttgtattt ggagcgctat gaaaaatcac ataatatcta aacta
//
LOCUS       DAJVYM010000399          524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVYM010000399 DAJVYM010000000
VERSION     DAJVYM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.404"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>524)
                     /locus_tag="OQS60_004187"
     CDS             complement(<1..>524)
                     /locus_tag="OQS60_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389775.1"
                     /translation="PRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAA
                     GIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYL
                     VSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFI
                     SFIALFTVCLHPTF"
BASE COUNT          179 a           96 c          131 g          118 t
ORIGIN      
        1 aaatgtggga tgcaggcaga cggtaaacag agcaataaat gagataaagg tgtacagcat
       61 cagtaatgtt gttgggcgaa cggtattacg aatgagtgga gcggtaataa atacacaaag
      121 cagcgaaccc acggtgtaga tactgagtag tttgatcgac atagtgtatg acatgcctgc
      181 aacaaattgt ccgtactgtg ccagccactg gctaaccaga taaaacgttg ccattgagat
      241 atagccatat aaggtataac tggctaaatc gataattgaa cagcgatgtt ccgtagagct
      301 ggtggttttc tttatgacag gtaagcgacg acccggatgg ggtgggaacg tacaacggta
      361 taaaaacaga gcgttaataa acataatgcc tgcagcaatc ataaaggacc aaccgaacca
      421 cagttcagcc cacactaaca ggctaatgat tagcggtaat aaaaattgtc cgctggaaac
      481 aaatgcttta attaaaatat tggctgtccc aggtgagcgt ggaa
//
LOCUS       DAJVYM010000400          521 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVYM010000400 DAJVYM010000000
VERSION     DAJVYM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.405"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>521
                     /locus_tag="OQS60_004188"
     CDS             <1..>521
                     /locus_tag="OQS60_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389776.1"
                     /translation="PVLSAQEAVNYIPDEATLCVLGAGGGILEATTLITALADKYKQT
                     QTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRISELAEQNKIIAYN
                     YPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKEDLIKLVEFDNKEY
                     LYYKAIAPDIAFI"
BASE COUNT          146 a          123 c          124 g          128 t
ORIGIN      
        1 gccggtcctg tcggcacagg aagcggtgaa ttatattccc gacgaagcaa cactttgtgt
       61 gttaggcgct ggcggcggta ttctggaagc caccacgtta attacagctc ttgctgataa
      121 atataaacag actcaaacgc cacgtaattt atcgattatt agtccgacag ggcttggcga
      181 tcgcgccgac cgtggtatta gtcctctggc gcaagaaggt ctggtgaaat gggcattatg
      241 tggtcactgg ggacaatcgc cgcgtatttc tgaactcgca gaacaaaata aaattattgc
      301 ttataactat ccacaaggtg tacttacaca aaccttacgc gccgccgcag cccaccagcc
      361 tggtattatt agtgatattg gcatcgggac atttgtcgat ccacgccagc aaggcggcaa
      421 actgaatgaa gtcacgaaag aagacctgat taaactggtc gagtttgata acaaagaata
      481 tctctattac aaagcgattg cgccagatat cgccttcatt c
//
LOCUS       DAJVYM010000401          520 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVYM010000401 DAJVYM010000000
VERSION     DAJVYM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.406"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>520
                     /locus_tag="OQS60_004189"
     CDS             <1..>520
                     /locus_tag="OQS60_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS2389777.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJVYM010000402          514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVYM010000402 DAJVYM010000000
VERSION     DAJVYM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.407"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          137 a          110 c          129 g          138 t
ORIGIN      
        1 cgttttatca aaaatataag agacaaattc ttctttagtc atatagattt ccttcttggt
       61 tattcgggat aagaaggata ccacctcgcc tgacgtggtt aaaagcaggc acacaacgcg
      121 aaatgacgcg gcggtaagta tggcggagtt attccttccc cgttgaggac accgggttgt
      181 caggttgacc atacgcttaa gtgacaacca cgccacaaca ctccatgttg attggtacct
      241 ttggcggcat cagtttcatt gctggctgat gtccgccctt tttaaagtga attttgtgat
      301 gcggtgaatg cggctcagcg cacgcggcac agttaaaaag cgattagttc ccccgtatcg
      361 ggtggttatg ggtttccctg tatccggcgt taattgttaa ctggttaacg tcacctggag
      421 gcaccaggca ccgcatcaac aaagttcact tcggtgatga aaggaaagag aaaatgttga
      481 atgtagctat tgaaaaccag aacgggtgga atta
//
LOCUS       DAJVYM010000403          511 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVYM010000403 DAJVYM010000000
VERSION     DAJVYM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.408"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>511)
                     /locus_tag="OQS60_004190"
     CDS             complement(<1..>511)
                     /locus_tag="OQS60_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS2389778.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVA"
BASE COUNT          137 a          140 c          119 g          115 t
ORIGIN      
        1 gccacatcct gtggccccac atacggcgca ggttttccgg tcagatgcac cgcaaccacg
       61 cccggatagt cgatatccca ggtgtcatta agcagctgtt ttaccaactc accgccaccc
      121 tcaccgactg ccattgtccc taatgcaccg taacgggtgt ggctgtctga cccgaggatc
      181 attttgcccc cgcctgccat catctcacgc atatattgat ggatgaccgc aatatgcgga
      241 ggcacaaaaa taccgccata acgctgagct gccgataaac caaaaacatg gtcatcaccg
      301 ttaatggtgc cgccgacggc gcagagtgag ttatggcagt tggtcagcac atacggcagc
      361 gggaaacgtt ccattccgga cgctttagcg gtctgtacaa taccaacaaa ggtaatatcg
      421 tgagaggcta atgaatcaaa cttaatttta agtttatcca tatttccgga cgtattatga
      481 gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAJVYM010000404          507 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVYM010000404 DAJVYM010000000
VERSION     DAJVYM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.409"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            223..>507
                     /locus_tag="OQS60_004191"
     CDS             223..>507
                     /locus_tag="OQS60_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2389779.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLD"
BASE COUNT          173 a           96 c          108 g          130 t
ORIGIN      
        1 cttaaagatt ttaacgatcg cttattgtaa aaataatagc aaagaaaatt gtaagataca
       61 gaccgtaaat aaaaacatct tccttggtca ggaaagaaat cttgaaagca tatctggcta
      121 cgacaaaatc gttgtcaaat ataataactg gatcaccaaa gataatggcg agtttgaact
      181 ggcggtccat taattatcat tgtaataaac gaaggaatag taatgaaaaa ggtatttgca
      241 aaatctcttc tggtcgcagc gatgttttct gttgcaggct ccgcattggc agtacaaaag
      301 gatattactg taacggccaa cgttgatgcc gctctggata tgacacagac cgataacacc
      361 gcgttgccga aagcagtaga aatgcaatat ctgccggggc agggtctgca atcttaccag
      421 ttgatgacca aaatctggtc taacgacgtt accaaagatg taaaaatgca gttagtttcc
      481 ccggcgcaac tggttcaaag cctggat
//
LOCUS       DAJVYM010000405          495 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVYM010000405 DAJVYM010000000
VERSION     DAJVYM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.410"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>495)
                     /gene="fimZ"
                     /locus_tag="OQS60_004192"
     CDS             complement(<1..>495)
                     /gene="fimZ"
                     /locus_tag="OQS60_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCS2389780.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAK"
BASE COUNT          151 a           91 c           94 g          159 t
ORIGIN      
        1 tttggcgtag tcgataagct ctacaattga atgcaaacct agtttgccat aaatattaga
       61 tttatgcgca ctaactgttt tattgctaag taataactta tcggcaattt ctttattaga
      121 taatccgcta accagataac gtaatatggt cacttcacga ttagatagca cagtgaccgt
      181 tgaactattc gtactacatt tattgctttt tatatagttg agcgtttcgc taggaaaaaa
      241 cgtgtatccg gagaggatca tctgaacggg atgaaaaata tcattctgat cattgcattt
      301 actgacaaaa ccgttagcac cagcttgtat cgctctgcca gcataaaagc attctgattt
      361 cgatgataaa aataacactt tcactgtgct ctggatttgt ttgatccttt tcaggaaggt
      421 aaaaccgtct gttccgggca agtctatatc cataatgatt aaatcaacag gacgggttcg
      481 gagataatcg atggt
//
LOCUS       DAJVYM010000406          491 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVYM010000406 DAJVYM010000000
VERSION     DAJVYM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.411"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            30..>491
                     /gene="mpaA"
                     /locus_tag="OQS60_004193"
     CDS             30..>491
                     /gene="mpaA"
                     /locus_tag="OQS60_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS2389781.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVV"
BASE COUNT          105 a          137 c          136 g          113 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt c
//
LOCUS       DAJVYM010000407          490 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVYM010000407 DAJVYM010000000
VERSION     DAJVYM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.412"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>490
                     /locus_tag="OQS60_004194"
     CDS             <1..>490
                     /locus_tag="OQS60_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2389782.1"
                     /translation="APDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVT
                     DCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHEMRYEYNAAGD
                     LTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYD
                     VLD"
BASE COUNT          119 a          143 c          143 g           85 t
ORIGIN      
        1 gcccctgacg gcgatatcac ccgctaccgt tatgataatc cacacagtga cttaccctgc
       61 gcaacggaag atgccaccgg cagccggaaa accatgacat ggagccgtta cggtcagttg
      121 ctgaccgtca ctgactgttc cggttatgta acccgttatg accatgaccg ttttggtcag
      181 gtgacggcgg tgcaccgcga ggaagggctg agccagtacc gcgcatacga cagccgtgga
      241 cagttaattg cggtgaaaga cacgcagggc catgaaatgc ggtatgaata caacgccgcc
      301 ggtgacctga ccgccgtcat tgccccggac ggcagcagaa acgggacaca gtacgatgcg
      361 tggggaaaag ccatctgtac cacgcagggc gggctgacgc gcagtatgga atacgatgct
      421 gccggacggg tcatccgtct gaccagtgaa aacggcagcc acaccacctt ccgttacgat
      481 gtactcgacc
//
LOCUS       DAJVYM010000408          483 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVYM010000408 DAJVYM010000000
VERSION     DAJVYM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.413"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OQS60_004195"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OQS60_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS2389783.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..>483)
                     /gene="fliA"
                     /locus_tag="OQS60_004196"
     CDS             complement(367..>483)
                     /gene="fliA"
                     /locus_tag="OQS60_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this sigma factor directs
                     late flagellar biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS2389784.1"
                     /translation="QEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKLGKL"
BASE COUNT          122 a          120 c          102 g          139 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctg
//
LOCUS       DAJVYM010000409          482 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVYM010000409 DAJVYM010000000
VERSION     DAJVYM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.414"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>482
                     /locus_tag="OQS60_004197"
     CDS             <1..>482
                     /locus_tag="OQS60_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCS2389785.1"
                     /translation="AAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQT
                     ISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTS
                     HWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGYDWTGMSYQ
                     "
BASE COUNT          115 a          118 c          136 g          113 t
ORIGIN      
        1 gcggcgcaac atcctgccag cttagtcagc gtgcgtccta atggtctgga agacaccgcg
       61 cagtttaaac tggaagttga ccaggaaaag gcgcaggcat taggtgtttc actttctgac
      121 atcaatcaga ccatttcaac ggcgctgggt gggacttacg ttaacgactt catcgaccgt
      181 ggtcgcgtga aaaaggtgta tgttcaggcg gatgccaaat tccgtatgct gccagaagat
      241 gtcgataaac tttatgtccg cagcgccaac ggcgaaatgg tgccgttctc tgcctttacc
      301 acttcacatt gggtgtatgg ctctccgcga ctggaacgct acaacggtct gccgtcaatg
      361 gagattcagg gggaagccgc gccaggaacc agttccggcg atgccatggc attgatggaa
      421 aaccttgcgt caaaattacc tgcgggcatt ggttatgact ggacaggtat gtcgtatcag
      481 ga
//
LOCUS       DAJVYM010000410          482 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVYM010000410 DAJVYM010000000
VERSION     DAJVYM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.415"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..115)
                     /locus_tag="OQS60_004198"
     CDS             complement(<1..115)
                     /locus_tag="OQS60_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS2389786.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..>482)
                     /gene="tppB"
                     /locus_tag="OQS60_004199"
                     /gene_synonym="ydgR"
     CDS             complement(220..>482)
                     /gene="tppB"
                     /locus_tag="OQS60_004199"
                     /gene_synonym="ydgR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="mutations in this gene confer resistance to the
                     toxic peptide alafosfalin in Salmonella typhimurium;
                     member of proton-dependent oligopeptide transport (POT)
                     system family; in Escherichia coli this gene is regulated
                     by OmpR although not via osmoregulation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide/tripeptide permease"
                     /protein_id="HCS2389787.1"
                     /translation="LMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDPLMSLEVYG
                     RVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT          133 a          121 c          113 g          115 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 ac
//
LOCUS       DAJVYM010000411          480 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVYM010000411 DAJVYM010000000
VERSION     DAJVYM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.416"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>480
                     /locus_tag="OQS60_004200"
     CDS             <1..>480
                     /locus_tag="OQS60_004200"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS2389788.1"
                     /translation="DSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSNFGIPASI
                     VAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRVKNMLP
                     SLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAFQKELH
                     "
BASE COUNT          129 a          125 c          113 g          113 t
ORIGIN      
        1 gacagcacct ggcacggctt caaaaacatc gataacgagc acatgtatct tgacccgatc
       61 aaagtcaccc tgctgactcc ggggatggaa aaagacggca ccatgagcaa ctttggtatt
      121 ccggccagca tcgtggcgaa atacctcgac gaacatggca tcgttgttga gaaaaccggt
      181 ccgtataacc tgctgttcct gttcagcatc ggtatcgata agaccaaagc actgagcctg
      241 ctgcgtgctc tgactgactt caaacgtgcg ttcgacctga acctgcgtgt gaaaaacatg
      301 ctgccgtctc tgtatcgtga agatcctgaa ttctatgaaa acatgcgtat tcaggaactg
      361 gctcagaata tccacaaact gattgttcac cacaatctgc cggatctgat gtatcgcgca
      421 tttgaagtgc tgccgactat ggtaatgact ccgtatgctg cgttccagaa agagctgcac
//
LOCUS       DAJVYM010000412          479 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVYM010000412 DAJVYM010000000
VERSION     DAJVYM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.417"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>479
                     /locus_tag="OQS60_004201"
     CDS             <1..>479
                     /locus_tag="OQS60_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2389789.1"
                     /translation="NRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVAETKPGLP
                     QDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGDLKAIVKT
                     SHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGVLEYVND"
BASE COUNT          124 a          108 c          128 g          119 t
ORIGIN      
        1 aatcgtcgct ttgattcctg cttcctgacg gcgaaaaaag cgattgaaag cggtaagctg
       61 ggagagattg ttgaagtgga aagccatttt gactattacc gcccggtggc agaaaccaaa
      121 cctgggttgc cgcaggatgg cgcgttctat ggccttggtg tgcatacgat ggaccagatt
      181 atttctctgt tcggtcgccc ggatcacgtc gcttatgaca tctgcagcct gcgtaataaa
      241 gccaatcctg acgacacctt tgaagcgcaa ctgttttatg gcgacctgaa agccatcgtc
      301 aaaaccagcc atctggtgaa aatcgattat ccgaaattta tcgttcacgg taagaaaggt
      361 tcgtttatta aatacggtat cgaccagcag gaaaccagcc tgaaggctaa tattatgccg
      421 ggcgaaccgg gattcgcagc ggatgattcg gtcggtgtgc tggagtatgt caatgacga
//
LOCUS       DAJVYM010000413          476 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVYM010000413 DAJVYM010000000
VERSION     DAJVYM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.418"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>476
                     /locus_tag="OQS60_004202"
     rRNA            <1..>476
                     /locus_tag="OQS60_004202"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJVYM010000414          474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVYM010000414 DAJVYM010000000
VERSION     DAJVYM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.419"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>474
                     /locus_tag="OQS60_004203"
     CDS             <1..>474
                     /locus_tag="OQS60_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS2389790.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIER"
BASE COUNT          159 a          104 c          106 g          105 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgtt
//
LOCUS       DAJVYM010000415          471 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVYM010000415 DAJVYM010000000
VERSION     DAJVYM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.420"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            54..130
                     /locus_tag="OQS60_004204"
     tRNA            54..130
                     /locus_tag="OQS60_004204"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OQS60_004205"
     tRNA            173..248
                     /locus_tag="OQS60_004205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          131 a           99 c          117 g          124 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag t
//
LOCUS       DAJVYM010000416          470 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVYM010000416 DAJVYM010000000
VERSION     DAJVYM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.421"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            87..449
                     /locus_tag="OQS60_004206"
     CDS             87..449
                     /locus_tag="OQS60_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS2389791.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          110 c          138 g          108 t
ORIGIN      
        1 cggtggtgat gtctatctca caccgatgaa catgaccacg aaaccctccg atggcagtaa
       61 ggccggtaag cagaaggata acgccaatgc agacgaaaca acgtcttgat gtaccgctga
      121 gtctgaaatc tgtcagtgac tccggtgagt ttgaagggta tggctccgtc tttggtgtaa
      181 aggacagcca cgatgatgtg gtgatgtccg gggcatttgc tgcttccctg cgggcgtgga
      241 gtgacagaaa agcgttacct gcgctgctct ggcagcaccg catggatgaa cccatcggtg
      301 tttacaccga aatgaaggaa gacgatgtcg ggctttacgt caggggacgg ttgcttattg
      361 atgatgatcc cctcgcaaaa cgtgcacatg cacacatgaa ggccggttcg ttaaccggcc
      421 tttctattgg gtacgtcctg aaagactgag gtagcctgag tttaacggac
//
LOCUS       DAJVYM010000417          469 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVYM010000417 DAJVYM010000000
VERSION     DAJVYM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.422"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..355)
                     /locus_tag="OQS60_004207"
     CDS             complement(<1..355)
                     /locus_tag="OQS60_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2389792.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFREL"
BASE COUNT          128 a          117 c           99 g          125 t
ORIGIN      
        1 aaagttcgcg aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg
       61 ttgcaatagt gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat
      121 tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca
      181 tgcccagcag tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt
      241 cccgaatatg acggctgacg ccggactgtg aggtaaaaag catattcgca acctctgtca
      301 ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa
      361 actccgggca gttcagattt cccgttattg ttaaagtcta atgcccggca taacaaataa
      421 taaaaacccg catcttattc catcccgata taacgcttag ctcaccaat
//
LOCUS       DAJVYM010000418          467 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVYM010000418 DAJVYM010000000
VERSION     DAJVYM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.423"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(16..456)
                     /locus_tag="OQS60_004208"
     CDS             complement(16..456)
                     /locus_tag="OQS60_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCS2389793.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTPAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLIWKS"
BASE COUNT          123 a          119 c           91 g          134 t
ORIGIN      
        1 gtcgatacgc gctaatcagc ttttccatat cagcatgatc gaagcaaaga gttgcccctc
       61 taactcttct gaaaaggtca ctcccggata aatctccatt tcattgagtc gcttaatgga
      121 aaactgttta cgcagctcac ttcgatccag tgattccagt cggtcgattg tcgcggctaa
      181 ctcctgattc caggtcaatc ccaggaagcc atctatgcca tcttcgaggc tgtgttcacc
      241 aagcacggca cgactcagcg gatcattctt cgcgggatca aaagcagggg tgcctgtcag
      301 cccaaagtgt aaaaaatccc agcgtttatc aatatccagt cggcgtaatc ctgaaagcgt
      361 gtcatgtata ttatccatca aaggtttttc agtagactca agcagttggt taatcttttc
      421 tgagtcaatt tctgcaaaat aaccaatcat tcccatttat ttttcct
//
LOCUS       DAJVYM010000419          461 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVYM010000419 DAJVYM010000000
VERSION     DAJVYM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.424"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(91..>461)
                     /locus_tag="OQS60_004209"
     CDS             complement(91..>461)
                     /locus_tag="OQS60_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS2389794.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          135 a          124 c           81 g          121 t
ORIGIN      
        1 ccgctgccgc agaattagcg ctcggttaag ctgaagaaat aaggccggat gtatgaatta
       61 cgctccggcc tgttctcatt atttaaataa ctacgaaaaa tcaccccaca gtctcagctt
      121 gccgcagtgg agacagcgga agagataacc ctgacaatca ccaccctttt gcaggcattt
      181 agctagatca gaatttctaa taccgaaatt ctcacaatct tcttcaaggt tggcaaattc
      241 atcgaggcga tcttttatat cattccagcc cacatagccg ataaaagcac agaaatcgcc
      301 acaatgcgca agccagaatt cctgttgcca tccatgataa cctggcgtgc gttcaaccaa
      361 ctctttcagc atttcatcag gatatgtgtt cttaataccg gcaaattcgt cctcttcatc
      421 atactcaaat tcaacacctt ctatgctggc atcatcctga a
//
LOCUS       DAJVYM010000420          461 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVYM010000420 DAJVYM010000000
VERSION     DAJVYM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.425"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>461
                     /locus_tag="OQS60_004210"
     CDS             <1..>461
                     /locus_tag="OQS60_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2389795.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAJVYM010000421          457 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVYM010000421 DAJVYM010000000
VERSION     DAJVYM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.426"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..97
                     /locus_tag="OQS60_004211"
     CDS             <1..97
                     /locus_tag="OQS60_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase ynjE"
                     /protein_id="HCS2389796.1"
                     /translation="NVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(106..>457)
                     /locus_tag="OQS60_004212"
     CDS             complement(106..>457)
                     /locus_tag="OQS60_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS2389797.1"
                     /translation="LFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAA
                     KHQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR
                     VWSGYLTLKSLQRQ"
BASE COUNT          141 a          129 c          108 g           79 t
ORIGIN      
        1 gaatgtttcc gtgtatgacg gcggctggta cgaatggagc agcgatccaa aaaatccggt
       61 agcaaccggt gaacgcggcc cggacagtag caaataacat tgcaattact gacgctggag
      121 cgacttcagc gtcagatacc cactccacac ccgcgtaaag gtcgtcatcc agcacaacgc
      181 accgaagatc cacgcaaacc acgcaaacca tgcagggaat aaacatccca gcacaaacag
      241 taagatcgtt tctgtgcctt cggttaatcc ccccagatag taaaacgact tatgtgcata
      301 accggggtta tcaatctgat gttttgctgc cagtgcagca aaggcgagaa aactgctgcc
      361 cgtgccgata aacgcaaaca acaaccagcc gcctgccagc gcattttgct ccggtgcagc
      421 aagaataaag ccaaacggca ccagcgcgta aaagaga
//
LOCUS       DAJVYM010000422          457 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVYM010000422 DAJVYM010000000
VERSION     DAJVYM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.427"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..437)
                     /locus_tag="OQS60_004213"
     CDS             complement(<1..437)
                     /locus_tag="OQS60_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS2389798.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLL"
BASE COUNT          114 a          111 c          106 g          126 t
ORIGIN      
        1 tccagtaagc tgacatcaag ataatacccc tgtgacatcc cgctaatatt ggttgcacta
       61 ctggcatcgg actcattcgc tgggttcagg ttattagttg cactaatacc gtcaatcaaa
      121 taggcattct ggtagggcga cgcaccgtga atagagattt tctcagggcg aatatctccc
      181 tggttcaacg aggtactttg cgttgaatcc atgcgtaccg cagggttggt tctcagcaga
      241 tcgctgatat tgccattccc ggtcggtaaa cgctcgatgc tttcactggt gtaatgcgtg
      301 ttgccgttta cgggtacagg gacaggagca tagaccgtca tctcttcaaa aaaatgcatg
      361 tacatgtttt tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat gacgccagga
      421 ataagaactc gcttcattta tttatatctc cgtacag
//
LOCUS       DAJVYM010000423          456 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVYM010000423 DAJVYM010000000
VERSION     DAJVYM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.428"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>456
                     /locus_tag="OQS60_004214"
     CDS             <1..>456
                     /locus_tag="OQS60_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS2389799.1"
                     /translation="VESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTT
                     SFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIR
                     RLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          105 a          120 c          119 g          112 t
ORIGIN      
        1 cgttgagagt gaatatctcg aatactttgt tatcgacggc ccgacgccga aagcggtact
       61 tgatcgttat acccgcttta ctggtcgtcc ggcgctgccg cccgcgtggt ccttcggcct
      121 gtggctaacc acttcattta ccaccaacta cgacgaagcg acggtaaaca gctttatcga
      181 tggtatggcg gaacgcaatc tgccgctgca tgttttccac tttgactgtt tctggatgaa
      241 agccttccag tggtgcgatt ttgagtggga cccgctgact ttccctgacc cggaagggat
      301 gatccgccgc ctgaaagcga aagggctgaa aatctgcgtc tggattaacc cctatatcgg
      361 tcaaaaatcc cccgtcttta aagagttaca agagaaaggc tatttactca aacgcccgga
      421 cggttcgcta tggcagtggg ataaatggca gccagg
//
LOCUS       DAJVYM010000424          454 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVYM010000424 DAJVYM010000000
VERSION     DAJVYM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.429"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..386
                     /locus_tag="OQS60_004215"
     CDS             <1..386
                     /locus_tag="OQS60_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS2389800.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           95 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agca
//
LOCUS       DAJVYM010000425          450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVYM010000425 DAJVYM010000000
VERSION     DAJVYM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.430"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            28..>450
                     /locus_tag="OQS60_004216"
     CDS             28..>450
                     /locus_tag="OQS60_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2389801.1"
                     /translation="MRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYAL"
BASE COUNT          105 a          104 c          128 g          113 t
ORIGIN      
        1 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga taatcctgct
       61 atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga taaaggctac
      121 accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc tggccatgca
      181 tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc gccattaacc
      241 gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc tattcgcggt
      301 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
      361 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
      421 ttgggctttg agcatatcga ctatgcgctt
//
LOCUS       DAJVYM010000426          449 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVYM010000426 DAJVYM010000000
VERSION     DAJVYM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.431"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          133 a          103 c           91 g          122 t
ORIGIN      
        1 tcggaaagaa aaaaggccat cagcaaaact ctgatggcct gaatcacctt taccaatatt
       61 gtatgaaaaa acacgcacga ctaattgaca ataattttca tttccattaa aaaagatcgc
      121 gtagcattct taattcatga ggaactttac ccgccagcaa tctgagtagc gaaagctgtt
      181 catccccaat gtttttgctg gcgggtcctt ttttctacgg tcctcctccc cggagggggg
      241 catttcatta tttttctaat cgttactggt gagaaggcgg aagaaaaagc cagttcttcg
      301 cccctaaata acagcatgat tccagtccat gcaaaatctg atttccagct cacacatacc
      361 gagcgctgta caaaaaaccg ccagccaagg cagttaagag tgtgttcccg gggtttgctt
      421 agaatatttt gtaagttgtc cgaagagat
//
LOCUS       DAJVYM010000427          438 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVYM010000427 DAJVYM010000000
VERSION     DAJVYM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.432"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            6..221
                     /locus_tag="OQS60_004217"
     CDS             6..221
                     /locus_tag="OQS60_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS2389802.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            221..>438
                     /locus_tag="OQS60_004218"
     CDS             221..>438
                     /locus_tag="OQS60_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS2389803.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKA"
BASE COUNT          116 a           88 c          124 g          110 t
ORIGIN      
        1 gggatatgta tcagatggaa aaaatatcaa caggcattgc ctacggcacc tccgccggca
       61 gtgccggcta ctggtttttg cagtggttgg atcaggtcag tccatcacag tgggctgcga
      121 ttggtgtgct gggaagtctg cttctggggc ttctgactta tctgacaaat ctgtatttca
      181 aaataagaga agacaagcgt aaggctgcga gaggtgaata atgtcgccat cattacgcaa
      241 ggctgttgca gctgctattg gtggtggggc tgttgctata gcatctgtgt taatcactgg
      301 cccaggtggt aacgatggtc tggaaggtgt cagctacata ccatacaaag atatcattgg
      361 tgtatggact gtatgtcacg gacacaccgg aaaagacatc atgctcggta aaacgtatac
      421 cgaagcagaa tgcaaagc
//
LOCUS       DAJVYM010000428          438 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVYM010000428 DAJVYM010000000
VERSION     DAJVYM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.433"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>438
                     /locus_tag="OQS60_004219"
     CDS             <1..>438
                     /locus_tag="OQS60_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389804.1"
                     /translation="AGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQ
                     DLLFRTKAALQKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAA
                     QCHGGSPASVPWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQ"
BASE COUNT          104 a          103 c          139 g           92 t
ORIGIN      
        1 tgcggggatc ctgctggtac catgctgtcg tggtggttcg gcattcaccc agggcacgga
       61 ggggacattc agcgagtcca cgggggccag tcaggattcg gctcgctggg gagtgggtaa
      121 gccgttatat caggatctgc ttttccgcac gaaggcagca ttgcagaaaa acccgaaaaa
      181 cgttttgctg gcgatatgct ggatgcaggg ggaattcgat atgacgaatg ccagttacgc
      241 ccagcagcca gcagcatttc ttgcaatggt acagcagttc cgtgctgacc ttgccgggct
      301 ggcggcgcag tgtcacggtg gaagtccggc atcagtcccc tggatttgtg gcgacacgac
      361 atacgcgtgg aaacaagaac acggtacgca atatgaagtg gtatatggtg catataaagg
      421 taaagaatcc cagcagat
//
LOCUS       DAJVYM010000429          437 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJVYM010000429 DAJVYM010000000
VERSION     DAJVYM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.434"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>437)
                     /locus_tag="OQS60_004220"
     CDS             complement(<1..>437)
                     /locus_tag="OQS60_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2389805.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTW"
BASE COUNT           73 a          140 c          122 g          102 t
ORIGIN      
        1 ccatgttctg tctgccggga ttaccgcaaa atggttctcc agcgtggtgc ctgaacccct
       61 gcgtcccggt accgcctgcg gctgttcgcc gtgaagatcg ctcgccacca cctgccattc
      121 ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg gccatatctc
      181 cggcgaacgg cttgttccgc gcgccacttc tgcgttgttt cgccagccgt ccatctgcca
      241 gcgggcaaag ttctgcccgt gggcaccctt gaaacgtccg gggtagtcat acacttcata
      301 ctgcgtgcgc tggtaatcct ggtactggcc ttcctgatca aaacgccctg gccagccggg
      361 acgtttaaag gtgtagtctt tggtcactac ggaagaagga cggatttgtg cgctgtagcg
      421 aaactggctg atgcaga
//
LOCUS       DAJVYM010000430          425 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJVYM010000430 DAJVYM010000000
VERSION     DAJVYM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.435"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..254
                     /locus_tag="OQS60_004221"
                     /pseudo
     CDS             1..254
                     /locus_tag="OQS60_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           68 c           59 g          121 t
ORIGIN      
        1 tttacgagca aaagcggaaa ttgcttcgac aataattaaa tttttagagg ccaggataac
       61 caatctttca tacaatgaca tattaaaata tcagcaagaa ttccaaaaac aatgttataa
      121 gcaagttaaa gcctttacga cactctctcg atacaataaa attcaaacat gggcagaaat
      181 gtctgaatat cagttcgaag tatttcaata cgagacgctt aaccccaaaa aaatgtcaca
      241 cacgccttat ttaaagaggc cacttcctaa tgaaaaagat ataaattatg gggtggaaat
      301 agaaatacca tctggcaaaa gaattcgctt atcaaatcat tatcagaata taataccata
      361 atattgatta ttagcacttt ataattattg aatgaaaata gattttatgt aattttaaaa
      421 caaag
//
LOCUS       DAJVYM010000431          424 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJVYM010000431 DAJVYM010000000
VERSION     DAJVYM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.436"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            159..362
                     /locus_tag="OQS60_004222"
                     /pseudo
     CDS             159..362
                     /locus_tag="OQS60_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          110 a          103 c           96 g          115 t
ORIGIN      
        1 ggaacaaatt gccgcccact gtgagtcata ttcatcctga ctttccagaa ccatacgaat
       61 cgcccgctga cggacttcgg gggaaaaacg agtattttta gtcatcctgt ttacctcttt
      121 ctcagggagt ttagtctcca ggatttccgg ggcggttcag acatctagaa tttactgtca
      181 gcaccggcgt taaagtttac ttctgcgatc ctcagagtcc ttggcagcgg ggaacaaatg
      241 agaatacaaa tgggctaatt cggcagtact ttcctaaaaa gacatgtctt gcccaatata
      301 ctcaacatcg agtaggcagc ctgacggctg cggcttgtca tggtctgcaa ttaccgttat
      361 aaaaacaggc aatgtcattg tctttcaggt ggttatatgg cccgttcagc taaaccccgt
      421 aaac
//
LOCUS       DAJVYM010000432          420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJVYM010000432 DAJVYM010000000
VERSION     DAJVYM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.437"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(63..410)
                     /locus_tag="OQS60_004223"
     CDS             complement(63..410)
                     /locus_tag="OQS60_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCS2389806.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          126 a           98 c          112 g           84 t
ORIGIN      
        1 aaaaaggccc ctcccaacac atgggacaaa atgaaaggag gagcctcgga aaatactttt
       61 aattaatgtg cgttagacgc ggtgtggtta tccattgagt ggctctggcg ctcgtgcatt
      121 ttgcgagcgt agaacatcag gataatcagg ctgacgatga aggtacctgc cagctcgaag
      181 gagcttgcgc ccatcagcgc gatgaagcag aacacacaac ccagtacaga gcagatcagg
      241 ctgacaaagt tgcggatgtt aacgccttca aaacgaatca ggtcaacgca agagtagaag
      301 tacggcagca tagtcagcag tactgcgata ccggtcagtt caccgaacag gtcagatgct
      361 ttaccaccgg cagagttcat cagagtgatg aggatcatca gggcagtcat tttcactgca
//
LOCUS       DAJVYM010000433          416 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJVYM010000433 DAJVYM010000000
VERSION     DAJVYM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.438"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            30..206
                     /locus_tag="OQS60_004224"
     CDS             30..206
                     /locus_tag="OQS60_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389807.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          150 a           46 c           99 g          121 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcag
//
LOCUS       DAJVYM010000434          409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJVYM010000434 DAJVYM010000000
VERSION     DAJVYM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.439"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..163)
                     /locus_tag="OQS60_004225"
     CDS             complement(<1..163)
                     /locus_tag="OQS60_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCS2389808.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLG"
BASE COUNT          100 a          118 c           66 g          125 t
ORIGIN      
        1 cacccagcac cgggttcacc gggttagcat aggtaatccc tcccggacac accgaacagg
       61 ccactcccgt cggcgcacct atcagcacac ccgctgaacc ctgcacaata tccagtccct
      121 tgcgggtcat gtcgccctgc cgcgccgccg gttttccgct catggtttat tcctcttcat
      181 ctgaattgat ttacattagg ttggttattt tctcataccc acaactatat tttctctctg
      241 aatttattac gtgcaagaca atcgcagcac tctaactaac ttcgatgcaa tttgatgttc
      301 agaaaaatcc ctcattagat tattatcatg aaatacagct aatcatcttt agcttatata
      361 tttctcaatg tattcttttg aaaggatccg tgccctttca aactcttca
//
LOCUS       DAJVYM010000435          408 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJVYM010000435 DAJVYM010000000
VERSION     DAJVYM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.440"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          128 a           72 c           65 g          143 t
ORIGIN      
        1 aagtataaag ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag
       61 tcagacgata atttatcgat aatactggtc ggttttacat aaatcgacca gagaataaga
      121 tattactgtt gatgcttctg ctaattcatt atttatattt ataatttcaa ttttatctat
      181 aaaactaact atataaataa acataaccat gccgttgtgt tcgccctttg attagcaact
      241 ctggttttct ttcttagctg aacaatctgg ttatagcgtg cggactgacc gttaattttc
      301 tccttcttac tggataacac accatgagct gctaccttta aaacacctga atgtcaacag
      361 gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaat
//
LOCUS       DAJVYM010000436          407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJVYM010000436 DAJVYM010000000
VERSION     DAJVYM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.441"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          166 a           58 c           53 g          130 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atttttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacggg
//
LOCUS       DAJVYM010000437          406 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJVYM010000437 DAJVYM010000000
VERSION     DAJVYM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.442"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(4..>406)
                     /locus_tag="OQS60_004226"
     CDS             complement(4..>406)
                     /locus_tag="OQS60_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS2389809.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           85 a          110 c          136 g           75 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttct
//
LOCUS       DAJVYM010000438          406 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJVYM010000438 DAJVYM010000000
VERSION     DAJVYM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.443"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          134 a           75 c           89 g          108 t
ORIGIN      
        1 ctgctcatca aagaaggtgg tttcggtgat gcttgcaatc caaaagatat cttcattcat
       61 gccaccagcc caaacgcaac actaaagaca gcagttacag cggaatggtt tgaacgaaat
      121 aaagcggaat taggttattg gtagtccgag actttaagag aaattagaaa aacagcttat
      181 cagacgctct caatcgcatt ctaacgcaaa aaactatctt attttagggt gggtaatacc
      241 actgtacagg ctacccctga aaaagactgt ttagctcgtt acagagcgat tgaggaggtc
      301 tggtaaaaag gagattttaa aaatgaacga atatgaatta cagattttca ttgatagcga
      361 cactgctatg atggtgcagg ctttcgctga ttccggtgta tcaatt
//
LOCUS       DAJVYM010000439          403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJVYM010000439 DAJVYM010000000
VERSION     DAJVYM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.444"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(181..333)
                     /gene="hokE"
                     /locus_tag="OQS60_004227"
     CDS             complement(181..333)
                     /gene="hokE"
                     /locus_tag="OQS60_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS2389810.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT          108 a          113 c           87 g           95 t
ORIGIN      
        1 caaaatatct gtttatttat acagtaagtc ctgtttcgga cttctgttgg caacactcgt
       61 tattgctcgt aaaacgaaat cgcccgcagc catcactgac cgcgggcgaa agtgtaaagc
      121 aggtgcccta ccatcctgac ctgacaaccg gatatgcggg gattactccc cgcacaacgg
      181 ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt ccttcaccgt
      241 aaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac
      301 tatgactgcc gcaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg
      361 gactaccatc caaattgttc gggtttggag tggcagcccc agg
//
LOCUS       DAJVYM010000440          400 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJVYM010000440 DAJVYM010000000
VERSION     DAJVYM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.445"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..384
                     /locus_tag="OQS60_004228"
     CDS             <1..384
                     /locus_tag="OQS60_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS2389811.1"
                     /translation="TPNIFELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGN
                     EENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGL
                     RVLEVMRYTQQHESDELILPPLAEA"
BASE COUNT          103 a           89 c          118 g           90 t
ORIGIN      
        1 acccccaata tctttgagct ggaaatcctg accggtaaaa attgccgcga tctcgacagt
       61 gccattgctg ccgcaaaaag tctgctttca gacacattaa aatgggtggt gattaccagc
      121 gcctccggta atgaagaaaa tcaggagatg caggtggtgg tggtcagtgc cgacagcgtg
      181 aatgtcattt cccattcacg ggtaaaaacc gacctgaaag ggactggcga cctgttttgt
      241 gctcagctta tcagtggttt gctgaaaggg aaggcgttaa acgatgcggt gcaccgtgcg
      301 gggttgcgcg tactggaagt gatgcgctat acccagcagc atgagagcga tgaattgatt
      361 ttgccgccgc tggcggaagc ataaaaaatg gcgccgatgg
//
LOCUS       DAJVYM010000441          400 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJVYM010000441 DAJVYM010000000
VERSION     DAJVYM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.446"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            30..>400
                     /locus_tag="OQS60_004229"
     CDS             30..>400
                     /locus_tag="OQS60_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2389812.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPAL"
BASE COUNT          136 a           95 c           83 g           86 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct
//
LOCUS       DAJVYM010000442          395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJVYM010000442 DAJVYM010000000
VERSION     DAJVYM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.447"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            229..336
                     /locus_tag="OQS60_004230"
     CDS             229..336
                     /locus_tag="OQS60_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS2389813.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           99 a           97 c          119 g           80 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggatcaggcc gcagg
//
LOCUS       DAJVYM010000443          394 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJVYM010000443 DAJVYM010000000
VERSION     DAJVYM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.448"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..366
                     /locus_tag="OQS60_004231"
     CDS             <1..366
                     /locus_tag="OQS60_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS2389814.1"
                     /translation="DLKGKKVGVGLGTNYEEWLRQNVQGVDVRTYDDDPTKYQDLRVG
                     RIDAILVDRLAALDLVKKTNDTLAVTGEAFSRQESGVALRKGNEDLLKAVNDAIAEMQ
                     KDGTLQALSEKWFGADVTK"
BASE COUNT          111 a           80 c          117 g           86 t
ORIGIN      
        1 gatctgaaag gcaaaaaagt gggtgtcggt ctgggcacca actatgaaga gtggctgcgg
       61 cagaatgttc agggtgtcga tgtgcgtacc tatgatgatg acccgaccaa atatcaggat
      121 ctgcgcgtag gccgtatcga tgcgatcctc gttgaccgtc tggcggcgct ggatctggtg
      181 aagaaaacca acgatacgct ggcagtaacc ggtgaagcat tttcccgtca ggagtctggc
      241 gtggcgctgc gtaaaggaaa tgaagatctg ctgaaagcag tgaatgatgc aattgcggaa
      301 atgcaaaaag atggcactct gcaagccctt tccgaaaaat ggtttggtgc tgatgtgacc
      361 aaataatcag cataatgaca aaaaagggcg cttt
//
LOCUS       DAJVYM010000444          391 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJVYM010000444 DAJVYM010000000
VERSION     DAJVYM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.449"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>391)
                     /locus_tag="OQS60_004232"
     CDS             complement(<1..>391)
                     /locus_tag="OQS60_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS2389815.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAA"
BASE COUNT           71 a          134 c          116 g           70 t
ORIGIN      
        1 ggcggcttcg cggtggaacg tctgtgtgcg cccgaagcgg tccaccagcc cggtcagtac
       61 ccggtacggc ggcagcggcg caggcagcac ctcatccgct tccggcaccc gctcacacca
      121 gccgagcagc caccacggcc cctgcggact gtttgtcgcc agataacgat gcggacttaa
      181 gcggagttct tccggcagcg cctgccagag tgcggccagc cggtgacctt catccagttt
      241 cgccacgccg ccgcgcacca gccagagtga ttcgctgcgg ctgtaaccgt cctcaccggg
      301 aaacaggtgc tcaaaataca ggcttctgcc gccgttatca ctgagtatca gtgtgttatc
      361 gcgcagctgt aagcggatat ccgcaggcat t
//
LOCUS       DAJVYM010000445          390 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJVYM010000445 DAJVYM010000000
VERSION     DAJVYM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.450"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>390)
                     /locus_tag="OQS60_004233"
     CDS             complement(<1..>390)
                     /locus_tag="OQS60_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS2389816.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          120 a           78 c           82 g          110 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt
//
LOCUS       DAJVYM010000446          389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJVYM010000446 DAJVYM010000000
VERSION     DAJVYM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.451"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            251..>389
                     /locus_tag="OQS60_004234"
     CDS             251..>389
                     /locus_tag="OQS60_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS2389817.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAJVYM010000447          386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJVYM010000447 DAJVYM010000000
VERSION     DAJVYM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.452"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>386
                     /locus_tag="OQS60_004235"
     CDS             <1..>386
                     /locus_tag="OQS60_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389818.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAJVYM010000448          386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJVYM010000448 DAJVYM010000000
VERSION     DAJVYM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.453"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>386
                     /locus_tag="OQS60_004236"
     CDS             <1..>386
                     /locus_tag="OQS60_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS2389819.1"
                     /translation="GIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLN
                     QTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALF
                     MALRSQRPPAGLIHHSDRGSQYCAYD"
BASE COUNT           97 a          104 c          105 g           80 t
ORIGIN      
        1 tggcatcatc gttggtcgtg accgactggc acgtcttcgt aaggagctaa ggctacgctg
       61 taagcagaaa cgcaagttca gagcgactac gaactcgaac cacaatctgc cagttgcgcc
      121 aaatctgctg aaccagacgt tcgctcctac agcaccaaat caggtctggg tggcggacct
      181 gacgtatgtt gccacacagg agggatggtt gtacctcgct ggcatcaaag atgtttatac
      241 gtgcgaaatt gtcggctacg ccatgggaga gcgcatgaca aaagagctga caggtaaagc
      301 cctgtttatg gcgctcagga gccagcgccc acctgccggg ctaatccacc actctgatcg
      361 aggttcacag tactgcgcat acgatt
//
LOCUS       DAJVYM010000449          384 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJVYM010000449 DAJVYM010000000
VERSION     DAJVYM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.454"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            53..>384
                     /gene="yhaV"
                     /locus_tag="OQS60_004237"
     CDS             53..>384
                     /gene="yhaV"
                     /locus_tag="OQS60_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCS2389820.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          114 a           71 c           97 g          102 t
ORIGIN      
        1 ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa taatggattt
       61 tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg aaacctacga
      121 cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt atcagagaaa
      181 agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata tcacggtcaa
      241 tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg
      301 gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata gtgaaaaaga
      361 gaaagtcatc attctgggat ggat
//
LOCUS       DAJVYM010000450          382 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJVYM010000450 DAJVYM010000000
VERSION     DAJVYM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.455"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>382)
                     /locus_tag="OQS60_004238"
     CDS             complement(<1..>382)
                     /locus_tag="OQS60_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389821.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQF"
BASE COUNT          126 a          101 c           76 g           79 t
ORIGIN      
        1 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
       61 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
      121 ctgcacggag taagcgatac tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
      181 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
      241 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
      301 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
      361 tgatttaact aagcgtggga ag
//
LOCUS       DAJVYM010000451          382 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJVYM010000451 DAJVYM010000000
VERSION     DAJVYM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.456"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..382
                     /locus_tag="OQS60_004239"
                     /pseudo
     CDS             1..382
                     /locus_tag="OQS60_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          113 a           84 c           98 g           87 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cg
//
LOCUS       DAJVYM010000452          380 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJVYM010000452 DAJVYM010000000
VERSION     DAJVYM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.457"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            73..380
                     /locus_tag="OQS60_004240"
                     /pseudo
     CDS             73..380
                     /locus_tag="OQS60_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          103 a           90 c           92 g           95 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa
//
LOCUS       DAJVYM010000453          376 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJVYM010000453 DAJVYM010000000
VERSION     DAJVYM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.458"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>376
                     /locus_tag="OQS60_004241"
     CDS             <1..>376
                     /locus_tag="OQS60_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS2389822.1"
                     /translation="AEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
                     IQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFS
                     RRYDYLTEYVQVLRDLWGTGKSD"
BASE COUNT           72 a          107 c          104 g           93 t
ORIGIN      
        1 agcggagcat taccatttcg atttcgccct gtcgatgatc aaactgcgtg gctttggcgg
       61 caaaactgag ttctgggatc acaaccttga gtcgttcacc ttgatggcgg gtctggcagc
      121 cgtgacctcg cgcattcaga tttacgccac tgctgccacc ttaacgttac ctccggcaat
      181 cgtcgcccgt atggccgcaa ccatcgactc catctctggc gggcgttttg gcgtcaacct
      241 cgtgactggc tggcaaaagc ccgtgtatga gcagatgggt atctggcctg gcgatgacta
      301 tttctcccgt cgttacgact atctcaccga atatgttcag gtgctgcgcg acctgtgggg
      361 cacggggaaa agcgat
//
LOCUS       DAJVYM010000454          374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJVYM010000454 DAJVYM010000000
VERSION     DAJVYM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.459"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>374)
                     /locus_tag="OQS60_004242"
     CDS             complement(<1..>374)
                     /locus_tag="OQS60_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389823.1"
                     /translation="QIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVTTQAMQ
                     SGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQIITI
                     VCRDGIAITDSIITQFKASKEHT"
BASE COUNT          107 a           81 c           69 g          117 t
ORIGIN      
        1 gtatgctctt tactcgcttt aaactgagta atgatactat cagtgatagc tattccatct
       61 ctacaaacaa tagtgatgat ttgcttctgg tctcgccatt gttcaaccgt agaaataaag
      121 gtttctacaa gttgcccctg tctggttaat agaactgaac ccgtcgaatc aaccactaca
      181 acaccgctaa tagagatggt cttaggtgct ctctgaacat tatcagtgat agtttgcccc
      241 gattgcatag cctgagttgt caccatcatc ggtgagtcat agtccatctg ttcagtagct
      301 gttaaggcaa gaaaagattc cacattatct ctaaggtgaa aatagatacc atctttacct
      361 gagtatctaa tttg
//
LOCUS       DAJVYM010000455          360 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJVYM010000455 DAJVYM010000000
VERSION     DAJVYM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.460"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(10..165)
                     /locus_tag="OQS60_004243"
     CDS             complement(10..165)
                     /locus_tag="OQS60_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS2389824.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT           90 a           69 c           89 g          112 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
//
LOCUS       DAJVYM010000456          357 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJVYM010000456 DAJVYM010000000
VERSION     DAJVYM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.461"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..332)
                     /locus_tag="OQS60_004244"
     CDS             complement(<1..332)
                     /locus_tag="OQS60_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS2389825.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVR"
BASE COUNT           98 a           89 c          104 g           66 t
ORIGIN      
        1 cggacagcct cacttcccag gcgctggcgg gcctgaataa cggcactggg ggcaacgaag
       61 gggcgattgc ccggcagcat gatgtccagg cgattcacaa tctggtgaag aggttcttta
      121 cgctcaagcg ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg
      181 cgtagcgtta cagtacctga ttcggcaagg caacgagaga tgagttcggg gtcgaggtaa
      241 tcccccagag aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc
      301 tgtccaatgt gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacaca
//
LOCUS       DAJVYM010000457          356 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJVYM010000457 DAJVYM010000000
VERSION     DAJVYM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.462"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT          137 a           63 c           35 g          121 t
ORIGIN      
        1 agttaacatg tataccttta tgtcctcact gtcatatatt tccataataa ttaaccacca
       61 aatatccatt taatgaataa atacagtgcc ataaaggtcc aagtaataag cacacattta
      121 gcctgaaaca ttatcattca tgtcaccaaa tcgtttattt agtgagataa aactagcata
      181 ttcgtaagat aaaaaatgca catggcaatc aagaaatgat attattactt ttttgatgta
      241 aaactattta gttttaaaat tcattttttc aacatataga gcattcattt tatgaatgca
      301 aaaaaacata cctgacacac aaattactct cctctgtaat attttctcat taacac
//
LOCUS       DAJVYM010000458          356 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJVYM010000458 DAJVYM010000000
VERSION     DAJVYM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.463"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>356)
                     /locus_tag="OQS60_004245"
     CDS             complement(<1..>356)
                     /locus_tag="OQS60_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2389826.1"
                     /translation="TMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLP
                     ELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITYIPENILSLDPTCT
                     IILEDNPLSSRIRESLS"
BASE COUNT           93 a           66 c           95 g          102 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggt
//
LOCUS       DAJVYM010000459          354 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJVYM010000459 DAJVYM010000000
VERSION     DAJVYM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.464"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>354
                     /locus_tag="OQS60_004246"
     CDS             <1..>354
                     /locus_tag="OQS60_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389827.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAID"
BASE COUNT           95 a           84 c           94 g           81 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gacc
//
LOCUS       DAJVYM010000460          354 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJVYM010000460 DAJVYM010000000
VERSION     DAJVYM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.465"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            16..>354
                     /locus_tag="OQS60_004247"
     CDS             16..>354
                     /locus_tag="OQS60_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2389828.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEES"
BASE COUNT           85 a           81 c          113 g           75 t
ORIGIN      
        1 taaggctaaa aaatcatgac gataagtttt aatactgttc cgtcgaatac gctggtgccg
       61 ttgttctacg ccgagatgaa caattctgcg gcaaatacag cggtgaccag cgcgcctgca
      121 ttactgatcg ggcatgccag caacgatgca gccattgagg ttaacagcct ggtgctgatg
      181 ccatcggcag attatgcccg ccagatttgt ggggcgggga gccagctggc gcgtatggtg
      241 gaagtctacc gtcagacaga tcctttcggt gaactgtatg ttattgcagt accggaagcc
      301 agaggggcgg cggcgacggt cagggtgacg gttgccggag aagcagagga aagc
//
LOCUS       DAJVYM010000461          352 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJVYM010000461 DAJVYM010000000
VERSION     DAJVYM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.466"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>352
                     /locus_tag="OQS60_004248"
     CDS             <1..>352
                     /locus_tag="OQS60_004248"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS2389829.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVW"
BASE COUNT          100 a           83 c           87 g           82 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg ca
//
LOCUS       DAJVYM010000462          352 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJVYM010000462 DAJVYM010000000
VERSION     DAJVYM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.467"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            119..>352
                     /locus_tag="OQS60_004249"
     CDS             119..>352
                     /locus_tag="OQS60_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389830.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEK"
BASE COUNT          112 a           81 c           92 g           67 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aa
//
LOCUS       DAJVYM010000463          351 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJVYM010000463 DAJVYM010000000
VERSION     DAJVYM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.468"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            286..>351
                     /locus_tag="OQS60_004250"
     CDS             286..>351
                     /locus_tag="OQS60_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCS2389831.1"
                     /translation="MTEIVADKTVEVVKNAIETADG"
BASE COUNT          127 a           61 c           65 g           98 t
ORIGIN      
        1 tcgctgcata gactgctgat ttttcgttct cttatcctgt gctcacgtgg ccttctggcg
       61 acgacgctca tccagcagaa atgaaaaata tcacccggca aaaaaataga atagaagcat
      121 tcgccataat gacattaaac attgtttgat atttatcata ttaatagaaa taaagacatt
      181 gacgcatccc gcccggctaa ctatgaatta gatgaagtaa aatttattaa tagttgtaaa
      241 acaggagttt cattacaatt tatatattta aagaggcgaa tgattatgac tgaaatcgtt
      301 gcagataaaa cggtagaagt agttaaaaac gcaatcgaaa ccgcagatgg a
//
LOCUS       DAJVYM010000464          342 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJVYM010000464 DAJVYM010000000
VERSION     DAJVYM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.469"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>342)
                     /locus_tag="OQS60_004251"
     CDS             complement(<1..>342)
                     /locus_tag="OQS60_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389832.1"
                     /translation="QYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRL
                     PYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASD
                     YLPQTNWSTTQ"
BASE COUNT           77 a           72 c           80 g          113 t
ORIGIN      
        1 attgagttgt tgaccaattt gtctgtggca ggtaatcact tgctgtcttt gtctccatcc
       61 ctggatactg gggatcatcc tgcggcatac aagtcagatt gggttttacc cacttcctgc
      121 aatacagtgt gtaatcgtaa gggcttaatg ctctgaagaa tgttttgaaa acattaggcg
      181 cactactggc ctggtcaata ggatatggca gtcttaattg tttcgctatg tctggcaact
      241 tacttggatc gggcgtaggc ggtttgctgg gtttcgctcc atatccgcca ttatttaagt
      301 gtccatattt agatgccagc cccattgaat ctatatactg aa
//
LOCUS       DAJVYM010000465          342 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJVYM010000465 DAJVYM010000000
VERSION     DAJVYM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.470"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..203
                     /locus_tag="OQS60_004252"
                     /pseudo
     CDS             1..203
                     /locus_tag="OQS60_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            complement(185..342)
                     /locus_tag="OQS60_004253"
                     /pseudo
     CDS             complement(185..342)
                     /locus_tag="OQS60_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="regulates genes involved in the biosynthesis and
                     transport of aromatic amino acids; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
BASE COUNT           81 a           85 c           89 g           87 t
ORIGIN      
        1 gccgtattgc gggtgttacc gatgtgcgta ctgtcccgtg gatgccttcc gaacgtgagc
       61 atctggcgtt gagcgcgtta ctggaggcgt tgcctgaacc tgtgctctct gtcgatatga
      121 aaagcaaagt ggatatggcg aacccggcga gctgtcagct ttttgggcaa aaattggatc
      181 gcctgcgcaa ccataccgcc gcaccggtca ggacgtgttg atcattttca tcctgaagat
      241 aaacaggcgt aatctccatc aggaaattct gcccattaat aacgacatgc tcgttatgcg
      301 aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa gc
//
LOCUS       DAJVYM010000466          341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJVYM010000466 DAJVYM010000000
VERSION     DAJVYM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.471"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..187
                     /locus_tag="OQS60_004254"
     CDS             <1..187
                     /locus_tag="OQS60_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS2389833.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT          102 a           79 c           89 g           71 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg a
//
LOCUS       DAJVYM010000467          334 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJVYM010000467 DAJVYM010000000
VERSION     DAJVYM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.472"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>334
                     /locus_tag="OQS60_004255"
     CDS             <1..>334
                     /locus_tag="OQS60_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS2389834.1"
                     /translation="EKAHELYDSIYGGKPAPDVINTLHKMAESGDIDAQSLLGWEYYQ
                     PRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYAKAYALFNQAAQH
                     GVNLAWSRL"
BASE COUNT           93 a           65 c           97 g           79 t
ORIGIN      
        1 cgagaaagcg catgaattgt atgacagcat atacggtggt aaacctgctc ccgatgttat
       61 aaatacactg cataaaatgg ctgaatcggg agatatcgac gcgcagagcc tgttaggctg
      121 ggaatattat cagcctcgtt atgataccaa acctgatgtt caggaagcga ttaaatggtt
      181 cgagttagcg gctaaacaag gtgacagaga agctccgtta gcgctggggg gtatctacta
      241 cgacggtgag caggtgcggg tggattatgc caaagcgtat gcactattta atcaggccgc
      301 gcagcacggt gtgaatttag catggtccag gttg
//
LOCUS       DAJVYM010000468          334 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJVYM010000468 DAJVYM010000000
VERSION     DAJVYM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.473"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(30..279)
                     /locus_tag="OQS60_004256"
                     /pseudo
     CDS             complement(30..279)
                     /locus_tag="OQS60_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          112 a           64 c           58 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta aagc
//
LOCUS       DAJVYM010000469          332 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJVYM010000469 DAJVYM010000000
VERSION     DAJVYM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.474"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..321
                     /locus_tag="OQS60_004257"
     CDS             <1..321
                     /locus_tag="OQS60_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389835.1"
                     /translation="VLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQVRCKI
                     CGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARIRRATK
                     SRTE"
BASE COUNT          104 a           62 c           92 g           74 t
ORIGIN      
        1 gttttatttc cggtaaaaga ggatgaccct cgtaatcagg ttaattttct ttatgagcca
       61 tcggaaagac catattgcca tcacgcctct gtacgggttg acgaaaaaga gcgtcaggtc
      121 cgctgtaaaa tctgcggtgc ggttgtggag ccgtttgact ggatgctctc tgtggcaaaa
      181 agagaaacca gactggcaga tgatgtaagg ctattgcgcc aggaggaaca ggaaaggcgg
      241 agaaatatag aaaagctgat acagattgag cgtaacgcga aagcgcggat acgcagggcg
      301 acaaaatcca gaactgaata attaaattta gc
//
LOCUS       DAJVYM010000470          332 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJVYM010000470 DAJVYM010000000
VERSION     DAJVYM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.475"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..300
                     /gene="bamD"
                     /locus_tag="OQS60_004258"
     CDS             <1..300
                     /gene="bamD"
                     /locus_tag="OQS60_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS2389836.1"
                     /translation="FSKLVRGYPNSQYTTDATKRLVFLKDRLAKYEYSVAEYYTERGA
                     WVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAANSSNT"
BASE COUNT          100 a           86 c           81 g           65 t
ORIGIN      
        1 ttttccaaac tggtgcgcgg ctatccgaac agtcagtaca ctaccgatgc caccaaacgt
       61 ctggtattcc tgaaagatcg tctggcgaaa tatgaatact ccgttgccga gtactataca
      121 gaacgtggtg catgggttgc cgtcgttaac cgcgtagaag gcatgttgcg cgactacccg
      181 gatacccagg ctacgcgtga tgcgctgcca ctgatggaaa atgcataccg tcagatgcag
      241 atgaatgcgc aagctgaaaa agtagcgaaa atcatcgccg caaacagcag caatacataa
      301 cagaaacctg aaacacaaaa cggcagccct tg
//
LOCUS       DAJVYM010000471          327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJVYM010000471 DAJVYM010000000
VERSION     DAJVYM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.476"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT           94 a           85 c           61 g           87 t
ORIGIN      
        1 cagagattcg aactctggaa ccctttcggg tcgccggttt tcaagaccgg tgccttcaac
       61 cgctcggcca cacttccgga atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc
      121 cctaatttgt ttgtttgcct gaaaaacagc caaaagtgca ttgatagcgt gaaataacag
      181 cagattgatc atttcatcac catgaattcc ttctctttta ctcgtttagc aaccggctaa
      241 acatccccac cgcccggcaa aaagaaaaat aggtccattt ttatcgctaa aagataaatc
      301 cacacagttt gtattgtttt gtgcaaa
//
LOCUS       DAJVYM010000472          323 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJVYM010000472 DAJVYM010000000
VERSION     DAJVYM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.477"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            252..>323
                     /locus_tag="OQS60_004259"
     CDS             252..>323
                     /locus_tag="OQS60_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HCS2389837.1"
                     /translation="MSGGDMILERVEIVGFRGINRLSL"
BASE COUNT           85 a           54 c           71 g          113 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttg
//
LOCUS       DAJVYM010000473          320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJVYM010000473 DAJVYM010000000
VERSION     DAJVYM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.478"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>320
                     /locus_tag="OQS60_004260"
     CDS             <1..>320
                     /locus_tag="OQS60_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecQ"
                     /protein_id="HCS2389838.1"
                     /translation="GNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFSDELVEAC
                     AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKVVDNPPQK
                     MQQN"
BASE COUNT           79 a           74 c           96 g           71 t
ORIGIN      
        1 agggaacttg cctgcgggtt tacaaggatc tacggggcga gtcctttctc gttggggaga
       61 ttccgggtgg ggaaagcagg tagcacagga gaaaaaaacg gggcgattca gtgatgagct
      121 ggtggaggca tgtgcggaaa tggttcgcca acgctggaat ccgcatcctg aaccaacctg
      181 ggtatgctgc gttccttcat taaagcacct cgacctggtt cctgattttg cccggcgact
      241 ggcggcgaaa cttggcttac cttttattga tgccattgaa aaagtcgtgg acaatccacc
      301 gcagaaaatg cagcaaaacc
//
LOCUS       DAJVYM010000474          320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.479, whole genome shotgun sequence.
ACCESSION   DAJVYM010000474 DAJVYM010000000
VERSION     DAJVYM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.479"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(<1..>320)
                     /locus_tag="OQS60_004261"
     CDS             complement(<1..>320)
                     /locus_tag="OQS60_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCS2389839.1"
                     /translation="PGGAPANVAVGIARLGGISGFIGRVGDDPFGALMQRTLLTEGVD
                     ITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCWRHGEWLHLCSI
                     ALSA"
BASE COUNT           83 a           95 c           71 g           71 t
ORIGIN      
        1 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
       61 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      121 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      181 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
      241 tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc caccgcaacg
      301 ttagctggcg cgccgccagg
//
LOCUS       DAJVYM010000475          313 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.481, whole genome shotgun sequence.
ACCESSION   DAJVYM010000475 DAJVYM010000000
VERSION     DAJVYM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.481"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>313
                     /locus_tag="OQS60_004262"
     CDS             <1..>313
                     /locus_tag="OQS60_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389840.1"
                     /translation="DLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPL
                     QGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELF
                     LV"
BASE COUNT           89 a           67 c           70 g           87 t
ORIGIN      
        1 cgatctctat ttacttctgt gtcgggaaaa tgtacagaca tttacccgta aaatagcgat
       61 ggtaaacgat tttgacagca gctttggcta ccgcaatctg cgcatccata agtctgccat
      121 ttgtgaacct ttgcaggggg aaaacgcctg gagttacgca gaaaaactga aactggcgat
      181 ttccagtatc cgtgaccata tgttctccga gtttattttc tgtgatgacg cgaaactcaa
      241 cgaaatagaa gagaatatct ggattgcgcg taatattcgc catgcaatgg aaattggcga
      301 actattcctc gtc
//
LOCUS       DAJVYM010000476          313 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJVYM010000476 DAJVYM010000000
VERSION     DAJVYM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.482"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(76..>313)
                     /locus_tag="OQS60_004263"
     CDS             complement(76..>313)
                     /locus_tag="OQS60_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS2389841.1"
                     /translation="EENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREH
                     CDELISIPMAGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT           84 a           93 c           61 g           75 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcc
//
LOCUS       DAJVYM010000477          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.483, whole genome shotgun sequence.
ACCESSION   DAJVYM010000477 DAJVYM010000000
VERSION     DAJVYM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.483"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>308
                     /locus_tag="OQS60_004264"
     CDS             <1..>308
                     /locus_tag="OQS60_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2389842.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJVYM010000478          304 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.484, whole genome shotgun sequence.
ACCESSION   DAJVYM010000478 DAJVYM010000000
VERSION     DAJVYM010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.484"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            77..>304
                     /locus_tag="OQS60_004265"
     CDS             77..>304
                     /locus_tag="OQS60_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS2389843.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT           98 a           68 c           71 g           67 t
ORIGIN      
        1 gctgattatt aaaaatcaac cacacccgcc agtattctgt atatttactg gcggtcatat
       61 cgtgagaggt atggcaatga atcttgtgac actcaaaacg tggggaaaac tcagatatcc
      121 ggataaccca ccatcaatat caacgctgag acgatgggca aggaatggaa acatttatcc
      181 tgcacctgaa ctacacggga ggagttacag ggtggttccg gaggctttct atatcaaccc
      241 aaataaggtt gataccgata taacacacca tcagcctaat gggcgacaag ggagagacag
      301 tccg
//
LOCUS       DAJVYM010000479          303 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.485, whole genome shotgun sequence.
ACCESSION   DAJVYM010000479 DAJVYM010000000
VERSION     DAJVYM010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.485"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
BASE COUNT           90 a           53 c           69 g           91 t
ORIGIN      
        1 atcattattt acgtatcagg aagagttaag ggtacaaggg gtactcgatg gcggtctata
       61 tttcatgact aacgccattt caaggaagaa gtgcggtggg tggtttcgaa tgagggctgg
      121 tgttttggta cgtggcacgc cactgcaatg tatttatcca tcagtactca ttcaaaagta
      181 cctggaaaaa atgcctgcta tgccaagcgg gcaaattgct tacatgtgga attaagtaat
      241 taacgataaa gccagagtat tatcatttat tatacccctt catttaatca ggttaattac
      301 tca
//
LOCUS       DAJVYM010000480          302 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.486, whole genome shotgun sequence.
ACCESSION   DAJVYM010000480 DAJVYM010000000
VERSION     DAJVYM010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.486"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            complement(61..297)
                     /locus_tag="OQS60_004266"
     CDS             complement(61..297)
                     /locus_tag="OQS60_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCS2389844.1"
                     /translation="MVFGEKLANILYEANSQFFYERNVIEEAVNALFCEREIINNKNI
                     IKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT           97 a           53 c           42 g          110 t
ORIGIN      
        1 gggactatat atttcgtttt cagataagac tccttgagag attatctttt atgtaaaatc
       61 tcaaatatca cctgatgtaa tgtcaatgat aatattaagt gcgctctgaa caacatcttt
      121 cttggtgtga ttaacatctg acaaaaagaa catcagcttc tttattatgt tcttgttgtt
      181 tattatttca cgctcacaga ataaagcgtt gactgcctct tctatgacat ttctctcgta
      241 aaaaaattga gagttagcct catataaaat attggccaat ttttcaccaa aaaccatatt
      301 at
//
LOCUS       DAJVYM010000481          301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.487, whole genome shotgun sequence.
ACCESSION   DAJVYM010000481 DAJVYM010000000
VERSION     DAJVYM010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.487"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>301
                     /locus_tag="OQS60_004267"
     CDS             <1..>301
                     /locus_tag="OQS60_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2389845.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAJVYM010000482          301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.488, whole genome shotgun sequence.
ACCESSION   DAJVYM010000482 DAJVYM010000000
VERSION     DAJVYM010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.488"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>301
                     /locus_tag="OQS60_004268"
     CDS             <1..>301
                     /locus_tag="OQS60_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2389846.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAJVYM010000483          299 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.489, whole genome shotgun sequence.
ACCESSION   DAJVYM010000483 DAJVYM010000000
VERSION     DAJVYM010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.489"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            19..>299
                     /gene="fimH"
                     /locus_tag="OQS60_004269"
     CDS             19..>299
                     /gene="fimH"
                     /locus_tag="OQS60_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCS2389847.1"
                     /translation="MAMACLCLANISWATVCANSTGVAEDEHYDLSNVFNSTNNQPGQ
                     IVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLN"
BASE COUNT           90 a           54 c           62 g           93 t
ORIGIN      
        1 ataatctgca gattattatt ggcaatggca tgtttgtgtc tggcaaacat atcctgggct
       61 actgtttgtg caaatagtac tggcgtagca gaagatgaac actatgatct ctcaaatgtc
      121 tttaatagca ccaataacca gccagggcag attgttgttt taccggaaaa atccggctgg
      181 gtaggtgtct cagcaatttg tccactcggc acgctggtga attatacata ccgtagttat
      241 gtcactaact ttattgttca ggaaactatc gataattata aatatatgca attaaatga
//
LOCUS       DAJVYM010000484          299 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.denovo.490, whole genome shotgun sequence.
ACCESSION   DAJVYM010000484 DAJVYM010000000
VERSION     DAJVYM010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.denovo.490"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..>299
                     /locus_tag="OQS60_004270"
     CDS             <1..>299
                     /locus_tag="OQS60_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2389848.1"
                     /translation="LGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGW
                     WAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVGLLLS"
BASE COUNT           57 a           66 c           90 g           86 t
ORIGIN      
        1 cgctcggcgg tgtcattttc ggtcactttg gcgaccgact gggacgtaag cgcatgttaa
       61 tgctgaccgt ctggatgatg ggcatcgcga cagccttgat tggtattctt ccttcattct
      121 cgaccattgg gtggtgggca cctattttac tggtgacact gcgtgtcatt cagggatttg
      181 ccgtcggcgg cgaatgggga ggcgcggcgt tgctttccgt tgaaagtgca ccgaaaaata
      241 aaaaagcctt ttacagtagc ggtgtacaag ttggctacgg tgtaggttta ctgctttca
//
LOCUS       DAJVYM010000485        16616 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.guided.087, whole genome shotgun sequence.
ACCESSION   DAJVYM010000485 DAJVYM010000000
VERSION     DAJVYM010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.guided.087"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            562..801
                     /locus_tag="OQS60_004271"
     CDS             562..801
                     /locus_tag="OQS60_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS2389849.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(989..1966)
                     /gene="intI2"
                     /locus_tag="OQS60_004272"
                     /pseudo
     CDS             complement(989..1966)
                     /gene="intI2"
                     /locus_tag="OQS60_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2298..2771
                     /locus_tag="OQS60_004273"
     CDS             2298..2771
                     /locus_tag="OQS60_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS2389850.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2866..3390
                     /gene="sat2"
                     /locus_tag="OQS60_004274"
     CDS             2866..3390
                     /gene="sat2"
                     /locus_tag="OQS60_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS2389851.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3448..4236
                     /gene="aadA1"
                     /locus_tag="OQS60_004275"
     CDS             3448..4236
                     /gene="aadA1"
                     /locus_tag="OQS60_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS2389852.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4312..4809
                     /locus_tag="OQS60_004276"
     CDS             4312..4809
                     /locus_tag="OQS60_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389853.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4870..5241
                     /locus_tag="OQS60_004277"
     CDS             4870..5241
                     /locus_tag="OQS60_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389854.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5651..6028
                     /locus_tag="OQS60_004278"
     CDS             5651..6028
                     /locus_tag="OQS60_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389855.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            6051..6203
                     /locus_tag="OQS60_004279"
                     /pseudo
     CDS             6051..6203
                     /locus_tag="OQS60_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6259..7875)
                     /locus_tag="OQS60_004280"
     CDS             complement(6259..7875)
                     /locus_tag="OQS60_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS2389856.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7876..9402)
                     /locus_tag="OQS60_004281"
     CDS             complement(7876..9402)
                     /locus_tag="OQS60_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS2389857.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(9405..11072)
                     /locus_tag="OQS60_004282"
     CDS             complement(9405..11072)
                     /locus_tag="OQS60_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS2389858.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(11069..13177)
                     /locus_tag="OQS60_004283"
     CDS             complement(11069..13177)
                     /locus_tag="OQS60_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS2389859.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(13164..13985)
                     /locus_tag="OQS60_004284"
     CDS             complement(13164..13985)
                     /locus_tag="OQS60_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS2389860.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(14144..15973)
                     /gene="glmS"
                     /locus_tag="OQS60_004285"
     CDS             complement(14144..15973)
                     /gene="glmS"
                     /locus_tag="OQS60_004285"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS2389861.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(16135..>16616)
                     /gene="glmU"
                     /locus_tag="OQS60_004286"
     CDS             complement(16135..>16616)
                     /gene="glmU"
                     /locus_tag="OQS60_004286"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS2389862.1"
                     /translation="IKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRPGAELLEG
                     AHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANKFKTIIGD
                     DVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWRRPVKKK"
BASE COUNT         4477 a         4035 c         3583 g         4521 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc cttgtgggcg
       61 gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt atttagggaa
      121 gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa aattaaaaat
      181 gacaaaatag tttggaacta gatttcactt atctggttaa ctaatctagc taacctgccg
      241 ctcagcttag tacgatccta tatccaactg atcataatcc tttcatcagt atcccaaatt
      301 aaatccccct cctagactgc ctcaccaaca ctttacagtg atcccgtatt tccggacagg
      361 tgattgattt ttttcgcttg tacaggtgtt aagccacagt tatcttgatg tggacattat
      421 gcattgtaat agcccattaa catgcgctaa gagcataaat taatatacag taacaagcat
      481 tacgaggtgc taatgtttgg aaagcagcct cttccaacta caataataag ttaactatcc
      541 tccacccaca aataattaca aatgacccct ttccgctata actccgatct caccagcggc
      601 tcgctacaaa cccgagagtg tcgcattatt actggcttgt tactccaaga gcttgatgaa
      661 gctgcctggg ataaagccat gtataaggaa aatgtgcttc agaaacgcac gcaatctacg
      721 gtaagacgta tttcttcagc gcttagaaaa cgcctagaac acctaagctc tgacttttgg
      781 gcttttgcgt ttttatgtta ggcattgtct ggcaactgga ggaagtgtaa tgaccatgaa
      841 tggtcactgt gattgacagt atcttgctta aacttatctg aacaatggta gaactggctc
      901 atatttatcc cctctatgat tgtttatctt tttcaccata aaaagtatca ggggctactc
      961 agggctacat aaagatattt atctactttt actgattgat aagtagcatc agtccatccg
     1021 caggactggt ggtgccggca aaatgctgac ccaacacatg cgtatagatt tgcgtggtct
     1081 taacatcgtt atgccctaag agttcttgca cagtgcgaat atcacgcccc gcttgtaata
     1141 gatgcgtagc aaacgagtga cgaaatgtat gacaagtgac acgcttgcta acgatgcctg
     1201 ctttttgtac ggctgccttc aatgcctttc gcgcaacgga gtcatgcaga tgatggcggc
     1261 ataatttgcc gttatacggg tggttgcaga gcgtgctgga gggaaagaca aacatccacg
     1321 ccgcttgtcg ataagcagaa gggtatttgt gatctaaagc aaaaggcagc gatggcccta
     1381 cgccttgtaa gttgtcgtct tgctgaataa gccgcgcttg ctcaatgagt tattttattg
     1441 ctgggattag gcgcgtgggc agtaggctgt ttctgctttt cccaccctta ccgtcatgca
     1501 cagtgatgca gccattatca aaatcaaaat ctttaacccg caaacgcaag cattcattaa
     1561 tgcgcaaacc tgcaccatac agcagcgtaa aaataacttg gttgcgagta tccataacct
     1621 gcaaaatgcg ttgcacttca tttgcagaga taacagaggg tagccgtcta ggcttgcttg
     1681 cagggatata atcaatatcg cccaacggct gttgtaaaaa cctgttgtac aaaaaagcta
     1741 gggcatttaa agcgattttc tgcgtgttta tggctacatg tctgctgttt gctaagctgg
     1801 ataaaaacag cctgacctct tcactgccca tggtctgagg atgacgtttt ttgtgaaaca
     1861 gaataaaacg cttaatccag tgcaggtaag ttttttcagt tttcagcgca taaccttttt
     1921 gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt agacataaaa cgctccttgt
     1981 cttgcaactg tctgcctata cagcctattc tagctgggat ttaaaaaagt gcctgttttt
     2041 tacgcctaga gatgcttgtt taccggtaga gttttaattt aatgctaaat aaattaaaat
     2101 gttatgagtt ctttgggtga gataatgtgc atcgtgcaag caggatagac ggcatgcacg
     2161 atttgtaata acagagtgtc ttgtattttt aaagaaagtc tatttaatac aagtgattat
     2221 attaattaac ggtaagcatc agcgggtgac aaaacgagca tgcttactaa taaaatgtta
     2281 acctctgagg aagaattgtg aaactatcac taatggtagc tatatcgaag aatggagtta
     2341 tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga acagctcctg tttaaagcta
     2401 ttacctataa ccaatggctg ttggttggac gcaagacttt tgaatcaatg ggagcattac
     2461 ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac atctgacaat gagaacgtat
     2521 tgatctttcc atcaattaaa gatgctttaa ccaacctaaa gaaaataacg gatcatgtca
     2581 ttgtttcagg tggtggggag atatacaaaa gcctgatcga tcaagtagat acactacata
     2641 tatctacaat agacatcgag ccggaaggtg atgtttactt tcctgaaatc cccagcaatt
     2701 ttaggccagt ttttacccaa gacttcgcct ctaacataaa ttatagttac caaatctggc
     2761 aaaagggtta acaagtggca gcaacggatt cgcaaacctg tcacgccttt tgtaccaaaa
     2821 gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc gtcatatgaa gatttcggtg
     2881 atccctgagc aggtggcgga aacattggat gctgagaacc atttcattgt tcgtgaagtg
     2941 ttcgatgtgc acctatccga ccaaggcttt gaactatcta ccagaagtgt gagcccctac
     3001 cggaaggatt acatctcgga tgatgactct gatgaagact ctgcttgcta tggcgcattc
     3061 atcgaccaag agcttgtcgg gaagattgaa ctcaactcaa catggaacga tctagcctct
     3121 atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc
     3181 gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt agagacacaa
     3241 acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct cggcggcatt
     3301 gacctgttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat gtactggtac
     3361 tggttctcgg gagcacagga tgacgcctaa caattcattc aagccgacac cgcttcgcgg
     3421 cgcggcttaa ttcaggagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     3481 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     3541 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     3601 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     3661 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     3721 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     3781 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     3841 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     3901 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     3961 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     4021 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     4081 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag gcaggcttat
     4141 cttggacaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     4201 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     4261 cgccgcttcg cggcgcggct taactcaagc gttagagagc tggggaagac tatgcgcgat
     4321 ctgttgaagg tggttctaag cctcgtactt gcgatggcat cggggcaggc acttgctgac
     4381 ctgccaattg ttttagtgga tgaagctcgt cttccctatg actactcccc atccaactac
     4441 gacatttctc caagcaacta cgacaactcc ataagcaatt acgacaatag tccatcaaat
     4501 tacgacaact ctgagagcaa ctacgataat agttcatcca attacgacaa tagtcgcaac
     4561 ggaaatcgta ggcttatata tagcgcaaat gggtctcgca ctttcgccgg ctactacgtc
     4621 attgccaaca atgggacaac gaacttcttt tccacatctg gcaaaaggat gttctacacc
     4681 ccaaaagggg ggcgcggcgt ctatggcggc aaagatggga gcttctgcgg ggcattggtc
     4741 gtcataaatg gccaattttc gcttgccctg acagataacg gcctgaagat catgtatcta
     4801 agcaactagc ctgctctcta ataaaatgtt aggcctcaac atctagtcgc aagctgaggg
     4861 gaaccactag tgtcatacga acctccaaga gacggttaca caaacgggta cattgttgat
     4921 gtcatgtatg acaatcgccc aagtaagtat ccagctgtgt tcagaacgta cgtccgaatt
     4981 ccagactatg tcgaactaga taacgcggag gagctggccg acgcaatcat tcgagaggtt
     5041 gagttaaagg aggcatggct cacaagggtc ttggggaact cagccgatcc tgacgcttct
     5101 gtgggcaagt gggaccggcg cctagcgcag tgcggtggta ttgatgagat gtcaagtggg
     5161 gtgcaggcaa catacttcag tccaaacggt cagctggtcg gcttagtggc atacattgat
     5221 gccgaatgga tggatgaata gctctgacgt tacgattcag gtgagacaac cagatgggca
     5281 ttgcgtacaa gctcagttgc caccgatgtg gctacgagtc tgatcttctt tacctaggcc
     5341 aaggtatggc gatgcttcct gagcaaatag tcggaacgtg cacatgctgc gacaatctaa
     5401 cgacgatttc tataaatgaa agctacggag tatgctcact ttgtggggct aaggatcgtg
     5461 tgatatttac aagctatcag catgaggcaa ggccaagaaa tccctgctat ctagagagaa
     5521 gtttcaaata tcaatgccca agatgccatg ccttcagtat ggagcctccg gatctaccgg
     5581 agatgctttg ggactaatgc tccgctttgc ctcgggtatc cgttcgacct atgtttaaga
     5641 ggaaacgcac gtgtctgccg ccaaagctct tgcctatgca ctttgtgaca agttggaaag
     5701 caaaaaagtt gatgaagtcg tacgaaatct tgtttctagc ggctggaata ttagggaagt
     5761 ggcctgggaa gcttcgtcct ttgatcatga aatgccctac tttgacacgg ggcgggactt
     5821 cgatattgga tttcctgaca cgctgtcatc caagattgag tttttgaccg cgaatggatc
     5881 aatcgtccaa gcacgtgctc gcctgcactt caagcaggct ctaatttttg ccaaggcgtc
     5941 caaacatgtc gccgcgctca agaactcatt ggattactac tatggatcgt ctgtaatccc
     6001 ccctcaaaac cacggaggct atttgtagaa aattccatta atatgaactc aaggtgttcc
     6061 ctacggacga atcagcgatg aaggtggtgt acctggctat ccaggcggcg accaagaaat
     6121 ggacgatgcc gatccgcaac tggaagccgg ccatgaaccg ttttatgatc gaatttggtg
     6181 accgtttaaa tggccacctt taactgaccg gcaaaagcac ttacacaaaa gggtttacag
     6241 gctctttcag tcattccttt aatgcgtaaa ttgctctcta accactctct cagcccaccc
     6301 ttccatatcc tctctcgata cttgcagctc ccccaacccc tttggatgat tcacccctct
     6361 atgcccgtct tgtccatata attgatcaaa caaactattt ggccaattca atgagctctt
     6421 caccactcca cgccgtatct tttcaaaatc attccgccat gtttcgctat caacgccact
     6481 caacactttg gtagaaagca ttttcacccc atccgatgca tccacctcta gcaatacaaa
     6541 ttcactgcca ttacgttgca ccccaacggc tttaatcact cttggtgagc catctttaca
     6601 cagatgtaat cggctacgcc caacttttgg caaaaccaag gtttcttcaa aaagcacccg
     6661 acatgcaaat ttagttttta gaattgaaag tagctcatca aaagcagcaa atcgattagc
     6721 aaaaatagag ttgtaattgg ttgcatcctg cttcccaccc acatctgccg ctgctaggac
     6781 accacctaag tgtggctcat cagtactaac caaattacta ttttcacttg ttgttgcttc
     6841 ttctttttct aaaatagttt ggctactttt tcttgccctg cgagattttt caattcgact
     6901 aggtctacta aaacttatcc atgtcggctc agcctctatg actcgtgtgt ctgtgtctat
     6961 attggctgtt tcctcatcgt ccaactgtag atgctcatca tcaacaactg actctgttga
     7021 aaccgctatg tgctgcgtac taccatcacc tgcctccttt tcctgaaaag aggcatggct
     7081 aatagctgtt gtgctaggaa gcatagcgtt gatttctaaa cctactattt cctcaactaa
     7141 ataatcctta tcctcgttag aagaacgtcc ttttacatgt aatttccaac cctgcatggg
     7201 cggagggtca aagctaaagc accagctttc aacgccgttc ttaatttctc tattttgttg
     7261 atagtgccta aaaatacttt catacgaatc cataacatct tgatccgaaa acaaccaaac
     7321 caaaagctgc actacggccg actgctctaa cgccccttta ggaaacgatg aactgggtaa
     7381 gatccttatc tctaaatgat ctcgctcaac ctcatactga acgtcgaact cttgctgtaa
     7441 agcggtactg ctcaaacagc ttcgacagaa ataggagtta attaaaaata atgaccgagc
     7501 tagctctaac tgtgggatgt gataacgagt cttgctatat ggtgtttcag cttcgtaaat
     7561 aaatgaactc tgcgctccat ccttgttgcg tacggaagca aaggacgaga gattaggaaa
     7621 atcgattatt ttggctcgtt gaagatccga tgcttcaaac tcaatcacac gatccgcctt
     7681 atttatttgc tttgttgtac tgttaaccac cttagctcga cttaataaag gcaaatgagt
     7741 aaatcgagtc catttccgtt cttgcattgg attaaaccaa acaaaaatac gccactcctt
     7801 atgacccgag ttacggaata aatgaccaat atgtacaacc tgcacattgt cattaaatgt
     7861 agctagccta accactcact cttccccata aaccatttcc aagaatcttt gtgcttcctc
     7921 agttatccgc tctttagata acgttgcact tcttaataat cgccagcgcc taagctcaac
     7981 atcttcctgt ttaagcttaa taaaagcttg gctaatccgt ctaatttgat aatcttccac
     8041 actctctgag taacgcttta agcaaagcgc taccaaaggc agtttctgta gattttttgc
     8101 aagagaggtt ccgttaggag tttgctttaa cagccagctc gatgtcgctc ttgggtgatc
     8161 aaggctacta tctagacgct ttatgattct taatagttgt cgtacagcaa ttcgatctct
     8221 ttggttccaa tcaactctag gggcgggtgc cagacgctct tgttgatgct gttgattcca
     8281 gtgaactagc caatccctgt catgtcggta aagccaagcg tatagcaccc caccctctaa
     8341 agactgtctt gccgccttaa ttccttggta tttatgcact agttgctgcc agtctttatg
     8401 cttaacagat aaatcttcag agttaggttg cacagactgg ctaacaggtc ttgtcgttat
     8461 agagtgctca gtaagagcac ttgcctgctg tagcgcttct ataaccgtta gttttggcaa
     8521 taaggcttgc cacacaatac tatgctgtaa ataactaaag gcttttctat gcttacggaa
     8581 tatacttttc agccaacacg tgtccttgtt ctctgccaac tttaaatcca gtttctctag
     8641 tgcctcatca ctaaaagtct gcctcactct ctccgccacc aagtcatgac gaatgtgctt
     8701 gcttttggtt agccctagat cctgcgctaa gcgctgataa aacagcgtcc actgctcaag
     8761 gcttggggaa agctcttgcg ctcgtggagc atctaacaga ggggctatat aagcagctag
     8821 tgctgttaat tgagataggg agtctttggg gtagtctgaa agcagctcag tatgacccaa
     8881 agcccaaaat tgatgtcggt gatcatctac agctctatca aagaagacta aagcaccgtg
     8941 ttttggacaa tatggcaaag cgggcaaata ccaatctcgt tgccaaaagg cttccccata
     9001 cctatttagc tgaagagcaa cgcaatcagg gcagtatcta aagcggttat cgctcttaac
     9061 tctagaagca gcgactccta gcattaaatg caccgcacct tgcgcttggt actccattaa
     9121 ccgaatagct tcgtctcggc gctccttgcc tacaaacgga gcatataaag ggaataaggt
     9181 atgctcataa ataagctgct gaacagcgta acgtcctgtt tgatgtagat gtcttgctat
     9241 cacacctaaa tgcgagggca gacctaaggt agcgaccacc ttgcggttgc catacacctc
     9301 atccaacagc tgcttaggac taacaatccc ttgataaacg cctgcccgtg caatagtgct
     9361 ataaatcagc tcattcgagt acggaacagg aaagtttctc atggctaacc tgcttgttta
     9421 aataagctcg ccatatccac tattacccct ttccctttta accgttcatg catggttttt
     9481 tcaggttggc gttgtgaata gatataacgt aaatccgtat caggcaagct atcccagtcg
     9541 ctgggcttga ctacctttat agccgaaacc tttttactct tggagggctt ttctagttct
     9601 gacactaccg tttcgccttc catcaaccac tgcaaaacaa gaggcagtaa cttttgtctt
     9661 gtcatcgttg gattctggct aaacgctttt ttaatagtgg gaattaacag gcttgaatcg
     9721 taatcctctt tcagcatcag atataaatga cgctgatctt cggtatctaa ctcttgaagg
     9781 gctttttctt ctggtgtttg ttcttgtatc gctgcgatat ctagctgaag ttggattaac
     9841 cgtttatcaa tctcgggaac gactagatca gaataacgag caatgcgttc tgggataccc
     9901 gagcgtaatg cctctagcat ggggtgcaca ggctttaact catcttgata cacttgccgc
     9961 aataaaccag cggtaatacg ctcattgcct aaagctagcg cacggagctg agcgagtaca
    10021 aaaagtttta ctacaatgtc catcactcct tggcttagct cataccacac atcacggacc
    10081 tcatccgata acagcgcatc tttgcgttgt aaaagctgta attgccaaag attatccgta
    10141 aaagcgatcc actcttgatt gggctttcca cgttgcgttt gttgtatagg atcccagaat
    10201 atagctccaa accctgcccc tctacgtgca gaccgcaaat cagcctcaaa aatctctcgt
    10261 gctttagggg taccaatcaa catcactggt acgccaataa tattcaccat cgtcacaaaa
    10321 aagttcagca tctcttgaga tccacccgaa cgagagcggc ttaaatgctg aatttcatca
    10381 ataaccaaca accctaaagc atgtgcattg gctatttgcg acatcaaagc caacatggtt
    10441 tctataccat gacgttttaa gccataacga cgctcatagt tcgagcccaa ggctcgatcc
    10501 aacgctctga aaaaattcaa gcagatttct tttagcgaac cattatgcga gcagtctatt
    10561 ttcaaataca ccacctgctc tacattgagt tcacgatggt aaatcacctg aggatacgtg
    10621 gctagaatac gatgaagaga ggtcgtcttc ccactaccag aacaaccaat taacaataag
    10681 ctttgtgccg tagatcgtgc ctcctcaaag cgaaatgtct ccaactctcc cgtttgaaca
    10741 cgctcataac cattttgtaa atgcttttgt aaatctcctg ttttaggatt tctgcctacg
    10801 tagccacctc gaatcatgac cgaaatacgc tcacttagta gcaaatgcgt acctaatggc
    10861 tgaaaatagt catctggaat acgacaaatg gtatgagctc tgataacacg ggacttttgc
    10921 aagtcagagg aagtaagctg taaagaggat ttcagtgatg cagcactatt cactgactct
    10981 tgtaatggtg gtaaggcctc gataaaagga ttatcacgat aagcctctac ccccgtatca
    11041 cgataaactg cttgaatccg ggtagcactc atgactcatc cttttctggt ggatcctgaa
    11101 ataattcagg cacgtatgtg ggtaggctgt aatcttcttg atcatccgct tccactgcgt
    11161 tgaaaggaat aactttagcc tcatcacctg aagagcttgg cttcaaatgc tccgcacgtt
    11221 ttttacgctc cgaggtcacg gcttcttttt tattagtttt aatctgctta atgcgtgttg
    11281 atttgggctc agtagtactg ggcgttaact tattcgcttt ctgaatggtt tgctgaataa
    11341 acgcctcaag ctccctgcgt ttagttaact catcctgctt cgcattggct ttattgtgtt
    11401 tttcttgtgc ttgtatatcc caaacctccc aaaatgagag acctttaaac tgccgactac
    11461 gttccgtcag attacagcgc caaaatacac ggctgccaac ttgcggaaac aaataaatcg
    11521 tatcaaccag cactgggtca taagccgctt ctaaatgttg aggtctagct atatcagtgc
    11581 tccgctgcaa ccaaccctca cgcagaatct ctgaccccga gtaatacaaa ccccacaaat
    11641 taacgccaaa tgaagaaata gagacctttc ggcgaggcag taacgctact cgcaactgct
    11701 cttgctccac agcccttaaa ctacctgtac gatgctgcat accccattgc catagctgga
    11761 caggaataga cggtaaatct gtaggaaaat cagcatctcg atcgtattta tccatcacca
    11821 gatggttatt tctgaataag atcgtacgca aaataatttg tgtgaactca aataccgaca
    11881 gagatgcatc taacctatag tctgtttcac catggctttt gatccgactg ccctctacaa
    11941 tgccaggtgc aaaggactta aactcggctt gtagtgttct aaaagtgctt tccactatgc
    12001 ctttagcatc gccacgtcta ggtggagcac tttccactcg cacattaaaa ctagaaacta
    12061 aggcttcgac ctgatgactc attaattcgc cacggtccgc tagcaacaca tctggcaaac
    12121 ctacacacgg ccagtctgag ctactaatct caatatcatg ctgggcacaa atggccgttt
    12181 tgtcagagca agcatttaca aaagcctgca tcgccaccac ataagacgga ttttcaaagc
    12241 cgatataaaa gcccgtgatc atccgactaa acacatcaat cacaatgtaa agcgttggtc
    12301 ttcctatgat tttttggcga tcatgatgat ccactaaata aatatcagca atcgtggcat
    12361 caatctcata acgactccca gggcctaacg cctgagaagt ggctgtacta cttaagggtc
    12421 gtacgtcttt tttatatacc cctgctttaa ctctagactt taagcgctga gctttagggt
    12481 attctcgatc ataaaaataa cgaaactgac gtagtgttgg gtaatcctct tggggaatgc
    12541 gaggaaaata ctgagcaaac aagtccacaa atcgtctata ggcaacggtg gtctttgtac
    12601 ctttttgatt taacaggtgc ttttctatgg tcaacctaaa aaggcgttca atctcgggcg
    12661 ttaccttggt tccttcaccc ttaccatatt ctctggctcg gccaatcttt gctgttcctg
    12721 tcgctgaacg tctttcccct ggtgcaccgc tgtttttgta gtcaggaatt aatgcattag
    12781 gcgtttgacc acgctgccag taacggcgta acaacttata aactgtagcc ttagtaacct
    12841 tatgttcttg gaccacatgc tcaacgagtt cgcttctgac tttagggtcg aaacgatcct
    12901 tactattaat aataggaaga atttttcgat aatcctcctc gcgcttctgg aagctgacag
    12961 aatctacaga cggctcttct aaatcaagat gtacataagg gtcatcagtt ctaactaagc
    13021 ggccttcatc taaatactgc attagcaaca actcagccct agcttgtgga actcctttat
    13081 cagcgcttat ttgcatccag acaacttggc catcctctat agccaaaatg cgatacggat
    13141 cattatcaaa tagcacaacc tcattaattt gccacatagc gcaactcctc catattcact
    13201 acttggctaa tacagagatc tgcacactta tttgccctga aagacttata aatattgaac
    13261 ttaataaaac catttgcggt taaggctcgt atctcactca atgttttgcc taactccaaa
    13321 tcataagcaa tatcaacctg cttacagaca ttgataatgt tttcatctcc tttttcttgc
    13381 aggatatggg ccaatgggga tagttgtgct aaaagctccg cagaaacttc ttctgttttc
    13441 actgaataaa gccattcaat attttctttt actacgggat ttatttcttt atcagtaaaa
    13501 atgaaccaag gaatttgctt ttgctgccaa tagcgacgct ctagttctag tttttctaag
    13561 gtacgctcgt cttgtaaggc tgctgcaggt ttgacttgaa tagcaaactg ctcaaaagga
    13621 ccatctttgc agtccactaa aaaatcagta gacataacct gatctacacc acgaataaca
    13681 ggatgcttaa taccactatc tattgcaatc tgcctggtat cactaggtaa taaggggaac
    13741 tgctcgcgta tatctagcac gctgctctcc cactcaagac tgagaaaaac agcaagctct
    13801 aagtcagata gcaaatgatg gactcgtccc gtcttatgag aataaatacg gtgggaacga
    13861 cctgaagaag gaacttcttg tactgttagc catggaatat agtctttacc atgcccttgg
    13921 ccacgcccct ctttaatacg acgggcaatt tgcacttcag aaaatgaaga gtttgcttta
    13981 gccataacaa aagtccagta tgctttttca cagcataact ggactgattt cagtttacaa
    14041 ctattctgtc tagtttaaga ctttattgtc atagtttaga tctattttgt tcagtttaag
    14101 actttattgt ccgcccacag ccctttttta tctatgatag tgattactca accgtaaccg
    14161 attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acatggtaag
    14221 ccagcagctg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca
    14281 tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat
    14341 acaattggcc gccacgcgcg cgaacttctt caatgttgga tttcagtttt tccagcaatt
    14401 cgttgttcgg tgcaacgacg ataaccggca tatcggcatc aataagcgcc agcggaccgt
    14461 gtttcagttc accagcagcg taggcttcag cgtgaatgta ggagatctct ttcagcttca
    14521 atgcgccttc cagtgcgatt gggtactgat cgccacggcc caggaacagt gcgtgatgtt
    14581 tgtcagagaa atcttctgcc agagcttcaa tgcgtttgtc ctgagatagc atctgctcaa
    14641 tacggctcgg cagcgcctgc agaccatgca caatgtcatg ttcaatggag gcatccagac
    14701 ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactga gtggtgaatg
    14761 ctttagtgga cgccacgccg atttccgtac ccgcgttggt catcagcgcc agatcggatt
    14821 cgcgcaccag agaagaaccc ggaacgttac agattgccag cgaaccaagg taacccagct
    14881 ctttcgacag acgcaggccc gccagggtat ccgcggtttc gccagactgt gacaaggtga
    14941 tcatcaggct gttacgacgc acggcagatt tacgatagcg gaattcagag gcgatttcga
    15001 cgtcgcacgg aatacctgcc agcgattcaa accagtagcg ggaaaccata ccggagttat
    15061 aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt
    15121 tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt
    15181 ttatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat
    15241 cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt
    15301 tatcgaagat gtttaccgaa cggcgagtga tttccgcaat atcgccctct tcaaggaaga
    15361 taaagcgacg ggtcaccggc aacagcgcca gctggtcaga agcgataaag ttttcgccca
    15421 tacccaggcc aatcaccagc ggactaccag aacgtgccgc cagcagggta tccgggtgac
    15481 gggagtccat gatcactgta ccgtacgcac cgcgcagctg cgggatagca cgcagaacag
    15541 cctcacgcag agtcccgcct tgtttcagct cccagttcac cagatgggca atcacttcgg
    15601 tgtcggtttc agaaacgaag gtatagccac gcgcttttaa ctcttcacgc agcggttcat
    15661 ggttttcgat gatgccgtta tgcaccacca caatgtgttc agaaacatgc ggatgcgcgt
    15721 tagcttctga aggttcaccg tgggtcgccc agcgagtgtg agcaataccg gtgccgccat
    15781 ccagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc
    15841 gggtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt
    15901 ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa
    15961 caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg
    16021 ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg
    16081 ggagggagat tatgttatct cctcatccca gatgaccgga ttacccggcc agaattactt
    16141 tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa
    16201 tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc
    16261 tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat
    16321 ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac
    16381 gttatcgcca atttccgcat cgcccaggta agtcagatgc ccgactttcg agcctttacc
    16441 cagacgcgct tttttcatct caacgaagtt accaacgtgt gcaccttcca gcaactcagc
    16501 accaggacgc aaacgagcaa acgggccaat agtacaggcc gctgccagat tcgcgtcttc
    16561 cacaacggta tacggactga tttcgcaatc atcgccaatc acgctgtttt taatca
//
LOCUS       DAJVYM010000486        10330 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.guided.135, whole genome shotgun sequence.
ACCESSION   DAJVYM010000486 DAJVYM010000000
VERSION     DAJVYM010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.guided.135"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            1..652
                     /locus_tag="OQS60_004287"
                     /pseudo
     CDS             1..652
                     /locus_tag="OQS60_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="OQS60_004288"
     CDS             649..1263
                     /locus_tag="OQS60_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS2389863.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OQS60_004289"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OQS60_004289"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS2389864.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="OQS60_004290"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="OQS60_004290"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS2389865.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="OQS60_004291"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="OQS60_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS2389866.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="OQS60_004292"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="OQS60_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS2389867.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="OQS60_004293"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="OQS60_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS2389868.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="OQS60_004294"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="OQS60_004294"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS2389869.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="OQS60_004295"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="OQS60_004295"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS2389870.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="OQS60_004296"
     tRNA            9156..9231
                     /locus_tag="OQS60_004296"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9837..9995
                     /locus_tag="OQS60_004297"
     CDS             9837..9995
                     /locus_tag="OQS60_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389871.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2589 a         2477 c         2661 g         2603 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatagctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9061 aagtagccat ttgattcata aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attaagaaaa
     9241 ttaataactt agcttaactt ctctaagaaa ttgtatcttc tgttatcact tacaaacgcc
     9301 tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg cgagtagttc ccaggctcaa
     9361 cctcgtaaca agttcctttg aagctggaag acgtgccacc gtctctaaac cttctaccaa
     9421 aactcttagc aagatgttcc acacctggcg cggtgtccaa tgatgctaca cgtcgtgaga
     9481 cgttgcagag gataaacagg ctaaatgatg ggaagagttt taagtgcaat agtggattaa
     9541 agcccacctc ttcaaaacct aacttgatcg ggaaagaatc aagctctagc acggtttcgt
     9601 ccaccggagt acaacgggtg cctgataaga gaagaactgc taagacttca tagcttttgt
     9661 gaaaaagccc tttttagtgg atacgcctac ggcgcttatt ttttggtata accatcaaag
     9721 gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg ttttatgcgt
     9781 agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg cacacgatga
     9841 aaaacattgc agcaatcaaa cgtaataacc gcaagattca cgctcgtaag ttcctgtcta
     9901 cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaacgaagaa agaaaactcc
     9961 ttagtgagtt gaaatggctt agagaggatt tctgaaaatc tccagaacgc aacaggacgc
    10021 attctaacgc tgttttcatt actcggtata gggataggtg atgtgtgttt aatgcgtctg
    10081 tgcgcttgtt ttgtgcctta ttttgcattt tcataatgcg gaagagaaca cggaaaacct
    10141 taaaattaat tattggggta tttaaggcta ttttcaggtg atttaagggt attaaacttg
    10201 atttaattga ttaaaatata aacagataat tatgtatctt attgatttat aaggttattt
    10261 ttaagtaaag gtaaataaaa aataaatagc cataaatagc tataaataca tcttaatacc
    10321 tatatccctt
//
LOCUS       DAJVYM010000487         5023 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.guided.203, whole genome shotgun sequence.
ACCESSION   DAJVYM010000487 DAJVYM010000000
VERSION     DAJVYM010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.guided.203"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..219
                     /locus_tag="OQS60_004298"
     CDS             <1..219
                     /locus_tag="OQS60_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HCS2389872.1"
                     /translation="FVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDEK
                     MGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            230..589
                     /gene="frdD"
                     /locus_tag="OQS60_004299"
     CDS             230..589
                     /gene="frdD"
                     /locus_tag="OQS60_004299"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS2389873.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            652..1785
                     /locus_tag="OQS60_004300"
     CDS             652..1785
                     /locus_tag="OQS60_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS2389874.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1874..2407
                     /gene="blc"
                     /locus_tag="OQS60_004301"
     CDS             1874..2407
                     /gene="blc"
                     /locus_tag="OQS60_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS2389875.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2404..2721)
                     /gene="sugE"
                     /locus_tag="OQS60_004302"
     CDS             complement(2404..2721)
                     /gene="sugE"
                     /locus_tag="OQS60_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS2389876.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2896..3042)
                     /gene="ecnB"
                     /locus_tag="OQS60_004303"
     CDS             complement(2896..3042)
                     /gene="ecnB"
                     /locus_tag="OQS60_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS2389877.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3153..3278)
                     /gene="ecnA"
                     /locus_tag="OQS60_004304"
     CDS             complement(3153..3278)
                     /gene="ecnA"
                     /locus_tag="OQS60_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS2389878.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3330..3896)
                     /gene="efp"
                     /locus_tag="OQS60_004305"
     CDS             complement(3330..3896)
                     /gene="efp"
                     /locus_tag="OQS60_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS2389879.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3938..4966
                     /gene="epmB"
                     /locus_tag="OQS60_004306"
     CDS             3938..4966
                     /gene="epmB"
                     /locus_tag="OQS60_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS2389880.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1253 a         1280 c         1276 g         1214 t
ORIGIN      
        1 ttcgtcgact ttttacaaaa cccggttatc gtgatcatta acctgatcac tctggcggca
       61 gctctgctgc acaccaaaac ctggtttgaa ctggcaccga aagcggccaa tatcattgta
      121 aaagacgaaa aaatgggacc agagccaatt atcaaaagtc tctgggcggt aactgtggtt
      181 gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc
      241 aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg
      301 gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct
      361 gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg
      421 tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca
      481 ccacgcgatg cacgatctga aaattcacgt gcctgcgggc aaatgggttt tctacggtct
      541 ggctgctatc ctgacagttg tcacgctgat tggtatcgtt acaatctaac gtatcgccaa
      601 tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct tatgttcaaa
      661 acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc ccctcaacaa
      721 atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt
      781 atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg ctatgcggac
      841 atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa
      901 acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat
      961 cccgcaacaa aatactggcc tgaacttact gctaaacagt ggaatgggat cacactatta
     1021 catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc
     1081 tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggctcc aggaacacaa
     1141 cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt
     1201 ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccatacg
     1261 tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg cgaaggtaag
     1321 gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt
     1381 gaagatatgg cccgctgggt gcaaagcaat ttaacccccc ttgatatcaa tgagaaaacg
     1441 cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag
     1501 ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat taacggcagt
     1561 gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc aactcctgca
     1621 gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg
     1681 tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta tcccaatcca
     1741 gcgagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt catcgggtcc
     1801 gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag
     1861 taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt
     1921 cgttgcctgc agttctccta cgccgccgcg tggcgtgaca gtagtgaata atttcgacgc
     1981 taaacgctat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg
     2041 actggaaaaa gtcaccgcaa catacagtct gcgtgatgac ggcggcctga atgtcatcaa
     2101 taaaggctat aaccctgaca gagaaatgtg gcagcagagt gaagggaaag cgtactttac
     2161 cggcgcacca acacgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta
     2221 taacgtcatt gcacttgatc gggaataccg ccatgcgctg gtttgcggcc cggaccgcga
     2281 ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac aggagatgct
     2341 ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac agcagcccgg
     2401 tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc
     2461 caggcgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg ccgcgccgac
     2521 tgcgccaata cccgtccaca cggcataagc cgtccctacc ggtaacgatt tcatcgccca
     2581 ggcaagtagc gccatactga ctatcatcgc cgtcacagta ataacacttg gcgtcaaacg
     2641 actaaagccg tgggtatatt tcaggccaac ggcccatacc acttccagca gaccagcaat
     2701 aactaagata atccaggaca tatcaggctc cggagaattg gggccatccc cggtgaaaag
     2761 aggcgtttgc aggtcgtcct gcaaagctga tggttgaacg gatattttgc caggtggcag
     2821 gggaatttca actattacct ttgtgactat cgcaaaaaaa caaacggcac gacataactg
     2881 tcgtaccgtt attgcttatt gctgcgcttt cgttgctgca ccagaaatcg cgttaccgcc
     2941 atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag
     3001 caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt ccttttaggt
     3061 aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg
     3121 aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcggga gatggagttg
     3181 ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt gagcagcgtg
     3241 ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg
     3301 tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag
     3361 cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg
     3421 ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga
     3481 tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca
     3541 ttccacagag ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt
     3601 gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca
     3661 ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca
     3721 gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga
     3781 gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg
     3841 ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg
     3901 ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa
     3961 taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctggtga
     4021 acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac gcagcgccaa
     4081 aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc
     4141 ggacgatcct attttgcgtc aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg
     4201 attctccacc gacccactgg aagaacagca cagcgtagtg cctggtttgt tgcataaata
     4261 ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt
     4321 ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc agactgcact
     4381 tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct
     4441 gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat
     4501 caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc
     4561 gctggttgaa cgctttgccc gttctacgct gcaaatcttg ctggtgaatc acatcaatca
     4621 tgccaatgag gtagatgaaa cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt
     4681 caccctgctt aaccagagcg ttctgttacg tggtgtgaac gataacgcac aaacgttggc
     4741 aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc atgtgctaga
     4801 taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc agattatgcg
     4861 tgagttgctg acattggtgt cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg
     4921 cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg
     4981 aagttaaaca ttagcctgat gcgctacgct tatcaggcct aca
//
LOCUS       DAJVYM010000488         3830 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.guided.234, whole genome shotgun sequence.
ACCESSION   DAJVYM010000488 DAJVYM010000000
VERSION     DAJVYM010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.guided.234"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            <1..184
                     /locus_tag="OQS60_004307"
     CDS             <1..184
                     /locus_tag="OQS60_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCS2389881.1"
                     /translation="TRATAHERTPQQERQKAAREAERGREAWTLGQGMKKPVAGCYGR
                     LTRWKGGGDVVYMALL"
     gene            complement(216..866)
                     /gene="tetR(A)"
                     /locus_tag="OQS60_004308"
     CDS             complement(216..866)
                     /gene="tetR(A)"
                     /locus_tag="OQS60_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS2389882.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            972..2171
                     /gene="tet(A)"
                     /locus_tag="OQS60_004309"
     CDS             972..2171
                     /gene="tet(A)"
                     /locus_tag="OQS60_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS2389883.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2138..2326)
                     /locus_tag="OQS60_004310"
                     /pseudo
     CDS             complement(2138..2326)
                     /locus_tag="OQS60_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2370..2759)
                     /locus_tag="OQS60_004311"
     CDS             complement(2370..2759)
                     /locus_tag="OQS60_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2389884.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2977..3417
                     /locus_tag="OQS60_004312"
     CDS             2977..3417
                     /locus_tag="OQS60_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS2389885.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          808 a         1097 c         1084 g          841 t
ORIGIN      
        1 gacccgcgcc acggcccacg agcggacgcc gcagcaggag cgccagaagg ccgccagaga
       61 ggccgagcgc ggccgtgagg cttggacgct agggcagggc atgaaaaagc ccgtagcggg
      121 ctgctacggg cgtctgacgc ggtggaaagg gggaggggat gttgtctaca tggctctgct
      181 gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt tctcggtcct
      241 tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc
      301 tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc
      361 ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc
      421 tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg
      481 tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc
      541 tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc
      601 gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc
      661 ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc
      721 cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt
      781 ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc
      841 actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa cataatatgt
      901 ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc
      961 ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact gtcgcgctcg
     1021 acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc
     1081 actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttgatgcaat
     1141 ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccggtcttgc
     1201 tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg cctttccttt
     1261 gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg
     1321 gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc ggcttcatga
     1381 gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg atgggcggtt
     1441 tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg
     1501 gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc
     1561 tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg
     1621 cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg gtcattttcg
     1681 gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc gcatttggca
     1741 ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa
     1801 ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg cttgccttcg
     1861 cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa
     1921 tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc
     1981 aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg
     2041 cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc
     2101 tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagaaattct tcaaattccc
     2161 gttgcacata gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt
     2221 aatactcgtc aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac
     2281 cagtatcccg atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg
     2341 caatactgtg tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa
     2401 atgcctgccg ttgctagaca ttgccagcca gtgcccgtca ctcccactct tacattgttc
     2461 gcagtatcta ggtacatgtt attgcgtgca ccttcatgat gtataatgtt attaatgaca
     2521 gcagaaagat cgacagaatt aatattcttc gtacctggga cagttatcgg gtgtcggcca
     2581 gtgatgagac caaaggatgc agtataagat gaaccactac tattatcatg gccaccatta
     2641 ccaccaccgt taaaactacc tcccgaactt ccagagccgc tatgaccatc attgtagggg
     2701 tcatttggtc ccatgtgacc aaaaccaccg acagtaccgc tatttaactc tgtactcata
     2761 aaattatcct ctttgacttt taaaaacagc aaaaactgta tttataacaa tgtgtttata
     2821 tacagtattc gaaatactac gcccgtcaaa accacaccgt caacaggttt tttgtgtatt
     2881 tttcacgcct cacgggcagc ctcagacctt ttgtgttaaa acagtaatat gttctgctgt
     2941 tacaatacgg tacacacagg agacactgtc acttttatgg catggcacac agttcaagag
     3001 acactgaaat taacaggaaa gtcgcgctcg caattatatc gtgacatggc taaaggcctt
     3061 gtcagctatc gcacaggaaa aaatgatcgc cgggagttcg agaccagtga actgattcgg
     3121 gcctatggcg agctgcgcac ttctgagaca ccaaaaaggc acaaggaggg acactccaaa
     3181 tctgtcgtta gccaggcgga aaatgctcag ctagacgtca ttcagcagaa actggacgct
     3241 ctgcagcaga ctgttaccct aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc
     3301 ctgcgaaatg aggagaggga acaactacag gccgaaatta gccgtctggc tgacgcgctg
     3361 gcacaggaaa aaaagcgggg attctggtcg cggctgtttc gctccggttc agagtaacag
     3421 tgcaggtgac tctgatttac cataacatct gttatcagca ccgcctgaga ggccctcagg
     3481 gcgtttctga cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac
     3541 gcgcaggctc tgtttttacg gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt
     3601 cacaccattc agccggggta agtcctgttt ccggcgttct tcccatccga aaacggtgaa
     3661 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg
     3721 tcagcacgtt caggaagggg gtgatgtaca aatggcacag aggatgaaag tgacgtatga
     3781 gctgaaggaa tgatgtaaaa gtgatgtata agtgatgtaa aagtgatgta
//
LOCUS       DAJVYM010000489         3628 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 100339
            SAMEA8899769-rid15856023.guided.237, whole genome shotgun sequence.
ACCESSION   DAJVYM010000489 DAJVYM010000000
VERSION     DAJVYM010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899769
            Sequence Read Archive: ERR6005440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478354.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478354.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 04:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,315
            CDSs (total)                      :: 4,241
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 112 of 234
            Pseudo Genes (incomplete)         :: 58 of 234
            Pseudo Genes (internal stop)      :: 87 of 234
            Pseudo Genes (multiple problems)  :: 23 of 234
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899769-rid15856023.guided.237"
                     /serovar="S. sonnei"
                     /isolate="100339"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Gambia"
                     /collection_date="2008-05-07"
     gene            816..1631
                     /gene="sul2"
                     /locus_tag="OQS60_004313"
     CDS             816..1631
                     /gene="sul2"
                     /locus_tag="OQS60_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS2389886.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1692..2495
                     /locus_tag="OQS60_004314"
                     /note="aph(3'')-Ib"
     CDS             1692..2495
                     /locus_tag="OQS60_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS2389887.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2495..3331
                     /locus_tag="OQS60_004315"
     CDS             2495..3331
                     /locus_tag="OQS60_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS2389888.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          778 a         1010 c          994 g          846 t
ORIGIN      
        1 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
       61 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      121 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      181 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      241 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      301 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      361 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      421 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      481 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      541 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      601 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      661 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      721 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      781 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
      841 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
      901 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
      961 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1021 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1081 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1141 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1201 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1261 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1321 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1381 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1441 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1501 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1561 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1621 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1681 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1741 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1801 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     1861 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     1921 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     1981 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2041 ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2101 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2161 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2221 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2281 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2341 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2401 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2461 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2521 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2581 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2641 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2701 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2761 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     2821 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     2881 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     2941 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3001 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3061 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3121 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3181 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3241 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3301 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3361 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3421 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3481 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3541 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3601 ccacgctaga tctatctcat ctgcgcaa
//
